id	detectionCriteria	pcrPlateID	decimalLatitude	geodeticDatum	materialSampleID	pcrProbeSeq	pcrProbeRepoter	ampVisMethod	basisOfRecord	quantificationCycle	coordinateUncertaintyInMetres	targetTaxonomicAssay	sampleMaterialProcess	pcrInhibitionScaleFactor	pcrProbeQuencher	associatedReferences	parentEventID	eventDate	pcrPrimerREV	biome	scientificName	pcrEfficiency	pcrAmpliconSize	catalogueNumber	sourceMaterialID	pcrInhibitionCheck01	occurrenceID	samplingProtocol	sampleCollectionMethod	eventID	eventTypeHierarchy	sampleName	assayType	occurrenceStatus	eventType	assayName	materialEntityID	pcrTargetGene	pcrCycles	eventHierarchy	coordinatePrecision	pcrInhibitionCheck	country	fieldNumber	pcrPrimerFWD	decimalLongitude
BA77683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05372	-34.83695	WGS84	BA77683	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.09000015	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_2	3/05/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA77683_Sspa_Oph2007_COI	A-BS-D2	1	BA77683_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA77683	Sample	BA77683	targeted	Present	Sample	Sspa_Oph2007_COI	A-BS-D2	COI	45	BA77683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A-BS-D2	GAAGGCCTTCCGATGATC	138.50729
BA83927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05434	-34.81176	WGS84	BA83927	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.725	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83927_Cmae_Bot2011_COI	A44	1	BA83927_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83927	Sample	BA83927	targeted	Present	Sample	Cmae_Bot2011_COI	A44	COI	45	BA83927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A44	ATGAACAGTCTATCCTCCTTTAG	138.52601
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83962_Cjap_Sim2018_COI	C4	1	BA83962_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Cjap_Sim2018_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83962_Esin_And2018_Cyb	C4	1	BA83962_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Esin_And2018_Cyb	C4	Cytb	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	ACCCCTCCTCATATCCAACCA	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83962_Mgig_Bot2012_COI	C4	1	BA83962_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Mgig_Bot2012_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	TCTTATTCGTTGGAGACTTTATAACCCT	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA83962_Mjap_Gib2022_COI	C4	1	BA83962_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Mjap_Gib2022_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	AATTTAATTGTGACTAGGCATGGTCTG	145.77379
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83963_Cmae_Bot2011_COI	C5	1	BA83963_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Cmae_Bot2011_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	ATGAACAGTCTATCCTCCTTTAG	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83963_Hsan_Wil2021_COI	C5	1	BA83963_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Hsan_Wil2021_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	CCTGGGCCGGTATAGTAGGT	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83963_Mros_Gib2022_COI	C5	1	BA83963_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Mros_Gib2022_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83963_Msal_Bot2012_COI	C5	1	BA83963_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Msal_Bot2012_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	GYTAGTTCCRATGATGTTAGCTG	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83963_Pvir_Dia2013_COI	C5	1	BA83963_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Pvir_Dia2013_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	CTTAGTGGCATTAATTCGDAATCC	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83963_Rhar_Sim2018_COI	C5	1	BA83963_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Rhar_Sim2018_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	CTGCTGCTATTGCTCATG	145.79636
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83965_Aamu_Bax2006_COI	C10	1	BA83965_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Aamu_Bax2006_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	GCACAACCGGGATCTTTACTTC	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83965_Pcan_Bot2011_IGS	C10	1	BA83965_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Pcan_Bot2011_IGS	C10	IGS	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	CGTAATCCTCAGTACTGGCTA	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83965_Pper_Dia2013_COI	C10	1	BA83965_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Pper_Dia2013_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	CTTAGTGGCATTAATTCGDAATCC	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83965_Sspa_Oph2007_COI	C10	1	BA83965_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Sspa_Oph2007_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	GAAGGCCTTCCGATGATC	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83965_Upin_Bot2011_COI	C10	1	BA83965_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Upin_Bot2011_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	CTTTAATTACAGCGTTTTTATTGTTGT	145.78334
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83966_Cjap_Sim2018_COI	C11	1	BA83966_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Cjap_Sim2018_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83966_Esin_And2018_Cyb	C11	1	BA83966_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Esin_And2018_Cyb	C11	Cytb	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	ACCCCTCCTCATATCCAACCA	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83966_Mgig_Bot2012_COI	C11	1	BA83966_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Mgig_Bot2012_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	TCTTATTCGTTGGAGACTTTATAACCCT	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA83966_Mjap_Gib2022_COI	C11	1	BA83966_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Mjap_Gib2022_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	AATTTAATTGTGACTAGGCATGGTCTG	145.7845
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83967_Cmae_Bot2011_COI	C12	1	BA83967_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Cmae_Bot2011_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	ATGAACAGTCTATCCTCCTTTAG	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83967_Hsan_Wil2021_COI	C12	1	BA83967_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Hsan_Wil2021_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	CCTGGGCCGGTATAGTAGGT	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83967_Mros_Gib2022_COI	C12	1	BA83967_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Mros_Gib2022_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83967_Msal_Bot2012_COI	C12	1	BA83967_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Msal_Bot2012_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	GYTAGTTCCRATGATGTTAGCTG	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83967_Pvir_Dia2013_COI	C12	1	BA83967_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Pvir_Dia2013_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	CTTAGTGGCATTAATTCGDAATCC	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83967_Rhar_Sim2018_COI	C12	1	BA83967_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Rhar_Sim2018_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	CTGCTGCTATTGCTCATG	145.78334
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83969_Aamu_Bax2006_COI	C17	1	BA83969_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Aamu_Bax2006_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	GCACAACCGGGATCTTTACTTC	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83969_Pcan_Bot2011_IGS	C17	1	BA83969_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Pcan_Bot2011_IGS	C17	IGS	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	CGTAATCCTCAGTACTGGCTA	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83969_Pper_Dia2013_COI	C17	1	BA83969_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Pper_Dia2013_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	CTTAGTGGCATTAATTCGDAATCC	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83969_Sspa_Oph2007_COI	C17	1	BA83969_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Sspa_Oph2007_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	GAAGGCCTTCCGATGATC	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83969_Upin_Bot2011_COI	C17	1	BA83969_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Upin_Bot2011_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	CTTTAATTACAGCGTTTTTATTGTTGT	145.78604
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83970_Cmae_Bot2011_COI	C18	1	BA83970_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Cmae_Bot2011_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	ATGAACAGTCTATCCTCCTTTAG	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83970_Hsan_Wil2021_COI	C18	1	BA83970_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Hsan_Wil2021_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	CCTGGGCCGGTATAGTAGGT	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83970_Mros_Gib2022_COI	C18	1	BA83970_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Mros_Gib2022_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83970_Msal_Bot2012_COI	C18	1	BA83970_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Msal_Bot2012_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	GYTAGTTCCRATGATGTTAGCTG	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83970_Pvir_Dia2013_COI	C18	1	BA83970_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Pvir_Dia2013_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	CTTAGTGGCATTAATTCGDAATCC	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83970_Rhar_Sim2018_COI	C18	1	BA83970_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Rhar_Sim2018_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	CTGCTGCTATTGCTCATG	145.78312
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83972_Aamu_Bax2006_COI	C23	1	BA83972_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Aamu_Bax2006_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	GCACAACCGGGATCTTTACTTC	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83972_Pcan_Bot2011_IGS	C23	1	BA83972_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Pcan_Bot2011_IGS	C23	IGS	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	CGTAATCCTCAGTACTGGCTA	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83972_Pper_Dia2013_COI	C23	1	BA83972_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Pper_Dia2013_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	CTTAGTGGCATTAATTCGDAATCC	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83972_Sspa_Oph2007_COI	C23	1	BA83972_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Sspa_Oph2007_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	GAAGGCCTTCCGATGATC	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83972_Upin_Bot2011_COI	C23	1	BA83972_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Upin_Bot2011_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	CTTTAATTACAGCGTTTTTATTGTTGT	145.78649
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83973_Cjap_Sim2018_COI	C24	1	BA83973_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Cjap_Sim2018_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83973_Esin_And2018_Cyb	C24	1	BA83973_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Esin_And2018_Cyb	C24	Cytb	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	ACCCCTCCTCATATCCAACCA	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83973_Mgig_Bot2012_COI	C24	1	BA83973_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Mgig_Bot2012_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	TCTTATTCGTTGGAGACTTTATAACCCT	145.78334
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83974_Cmae_Bot2011_COI	C28	1	BA83974_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Cmae_Bot2011_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	ATGAACAGTCTATCCTCCTTTAG	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83974_Hsan_Wil2021_COI	C28	1	BA83974_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Hsan_Wil2021_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	CCTGGGCCGGTATAGTAGGT	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83974_Mros_Gib2022_COI	C28	1	BA83974_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Mros_Gib2022_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83974_Msal_Bot2012_COI	C28	1	BA83974_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Msal_Bot2012_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	GYTAGTTCCRATGATGTTAGCTG	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83974_Pvir_Dia2013_COI	C28	1	BA83974_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Pvir_Dia2013_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	CTTAGTGGCATTAATTCGDAATCC	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83974_Rhar_Sim2018_COI	C28	1	BA83974_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Rhar_Sim2018_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	CTGCTGCTATTGCTCATG	145.77386
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83976_Aamu_Bax2006_COI	C30	1	BA83976_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Aamu_Bax2006_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	GCACAACCGGGATCTTTACTTC	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83976_Pcan_Bot2011_IGS	C30	1	BA83976_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Pcan_Bot2011_IGS	C30	IGS	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	CGTAATCCTCAGTACTGGCTA	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83976_Pper_Dia2013_COI	C30	1	BA83976_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Pper_Dia2013_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	CTTAGTGGCATTAATTCGDAATCC	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83976_Sspa_Oph2007_COI	C30	1	BA83976_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Sspa_Oph2007_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	GAAGGCCTTCCGATGATC	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83976_Upin_Bot2011_COI	C30	1	BA83976_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Upin_Bot2011_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	CTTTAATTACAGCGTTTTTATTGTTGT	145.7845
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83977_Cjap_Sim2018_COI	A85	1	BA83977_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Cjap_Sim2018_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83977_Esin_And2018_Cyb	A85	1	BA83977_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Esin_And2018_Cyb	A85	Cytb	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	ACCCCTCCTCATATCCAACCA	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83977_Mgig_Bot2012_COI	A85	1	BA83977_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Mgig_Bot2012_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	TCTTATTCGTTGGAGACTTTATAACCCT	138.52559
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83978_Cmae_Bot2011_COI	A86	1	BA83978_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Cmae_Bot2011_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	ATGAACAGTCTATCCTCCTTTAG	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83978_Hsan_Wil2021_COI	A86	1	BA83978_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Hsan_Wil2021_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	CCTGGGCCGGTATAGTAGGT	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83978_Mros_Gib2022_COI	A86	1	BA83978_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Mros_Gib2022_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83978_Msal_Bot2012_COI	A86	1	BA83978_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Msal_Bot2012_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	GYTAGTTCCRATGATGTTAGCTG	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83978_Pvir_Dia2013_COI	A86	1	BA83978_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Pvir_Dia2013_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	CTTAGTGGCATTAATTCGDAATCC	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83978_Rhar_Sim2018_COI	A86	1	BA83978_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Rhar_Sim2018_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	CTGCTGCTATTGCTCATG	138.5217
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83980_Cjap_Sim2018_COI	A88	1	BA83980_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Cjap_Sim2018_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83980_Esin_And2018_Cyb	A88	1	BA83980_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Esin_And2018_Cyb	A88	Cytb	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	ACCCCTCCTCATATCCAACCA	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83980_Mgig_Bot2012_COI	A88	1	BA83980_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Mgig_Bot2012_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	TCTTATTCGTTGGAGACTTTATAACCCT	138.51964
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83981_Cmae_Bot2011_COI	A89	1	BA83981_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Cmae_Bot2011_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	ATGAACAGTCTATCCTCCTTTAG	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83981_Hsan_Wil2021_COI	A89	1	BA83981_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Hsan_Wil2021_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	CCTGGGCCGGTATAGTAGGT	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83981_Mros_Gib2022_COI	A89	1	BA83981_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Mros_Gib2022_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83981_Msal_Bot2012_COI	A89	1	BA83981_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Msal_Bot2012_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	GYTAGTTCCRATGATGTTAGCTG	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83981_Pvir_Dia2013_COI	A89	1	BA83981_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Pvir_Dia2013_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	CTTAGTGGCATTAATTCGDAATCC	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83981_Rhar_Sim2018_COI	A89	1	BA83981_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Rhar_Sim2018_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	CTGCTGCTATTGCTCATG	138.52362
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83983_Aamu_Bax2006_COI	A91	1	BA83983_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Aamu_Bax2006_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	GCACAACCGGGATCTTTACTTC	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83983_Pcan_Bot2011_IGS	A91	1	BA83983_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Pcan_Bot2011_IGS	A91	IGS	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	CGTAATCCTCAGTACTGGCTA	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83983_Pper_Dia2013_COI	A91	1	BA83983_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Pper_Dia2013_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	CTTAGTGGCATTAATTCGDAATCC	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83983_Sspa_Oph2007_COI	A91	1	BA83983_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Sspa_Oph2007_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	GAAGGCCTTCCGATGATC	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83983_Upin_Bot2011_COI	A91	1	BA83983_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Upin_Bot2011_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	CTTTAATTACAGCGTTTTTATTGTTGT	138.52058
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83984_Cjap_Sim2018_COI	A92	1	BA83984_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Cjap_Sim2018_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83984_Esin_And2018_Cyb	A92	1	BA83984_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Esin_And2018_Cyb	A92	Cytb	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	ACCCCTCCTCATATCCAACCA	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83984_Mgig_Bot2012_COI	A92	1	BA83984_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Mgig_Bot2012_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	TCTTATTCGTTGGAGACTTTATAACCCT	138.51769
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.412	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83985_Cmae_Bot2011_COI	A93	1	BA83985_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Present	Sample	Cmae_Bot2011_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	ATGAACAGTCTATCCTCCTTTAG	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83985_Hsan_Wil2021_COI	A93	1	BA83985_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Hsan_Wil2021_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	CCTGGGCCGGTATAGTAGGT	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83985_Mros_Gib2022_COI	A93	1	BA83985_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Mros_Gib2022_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83985_Msal_Bot2012_COI	A93	1	BA83985_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Msal_Bot2012_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	GYTAGTTCCRATGATGTTAGCTG	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83985_Pvir_Dia2013_COI	A93	1	BA83985_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Pvir_Dia2013_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	CTTAGTGGCATTAATTCGDAATCC	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83985_Rhar_Sim2018_COI	A93	1	BA83985_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Rhar_Sim2018_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	CTGCTGCTATTGCTCATG	138.52075
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83987_Aamu_Bax2006_COI	A95	1	BA83987_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Aamu_Bax2006_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	GCACAACCGGGATCTTTACTTC	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83987_Pcan_Bot2011_IGS	A95	1	BA83987_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Pcan_Bot2011_IGS	A95	IGS	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	CGTAATCCTCAGTACTGGCTA	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83987_Pper_Dia2013_COI	A95	1	BA83987_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Pper_Dia2013_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	CTTAGTGGCATTAATTCGDAATCC	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83987_Sspa_Oph2007_COI	A95	1	BA83987_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Sspa_Oph2007_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	GAAGGCCTTCCGATGATC	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83987_Upin_Bot2011_COI	A95	1	BA83987_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Upin_Bot2011_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	CTTTAATTACAGCGTTTTTATTGTTGT	138.52086
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83988_Cjap_Sim2018_COI	A96	1	BA83988_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Cjap_Sim2018_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83988_Esin_And2018_Cyb	A96	1	BA83988_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Esin_And2018_Cyb	A96	Cytb	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	ACCCCTCCTCATATCCAACCA	138.51953
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA87563_Hsan_Wil2021_COI	D4	1	BA87563_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Hsan_Wil2021_COI	D4	COI	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	CCTGGGCCGGTATAGTAGGT	130.84495
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA87563_Mros_Gib2022_COI	D4	1	BA87563_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Mros_Gib2022_COI	D4	COI	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84495
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA87563_Pvir_Dia2013_COI	D4	1	BA87563_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Pvir_Dia2013_COI	D4	COI	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	CTTAGTGGCATTAATTCGDAATCC	130.84495
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA87563_Rhar_Sim2018_COI	D4	1	BA87563_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Rhar_Sim2018_COI	D4	COI	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	CTGCTGCTATTGCTCATG	130.84495
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA87565_Pper_Dia2013_COI	D6	1	BA87565_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Pper_Dia2013_COI	D6	COI	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	CTTAGTGGCATTAATTCGDAATCC	130.87387
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA87566_Cjap_Sim2018_COI	D10	1	BA87566_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Cjap_Sim2018_COI	D10	COI	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87402
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA87566_Esin_And2018_Cyb	D10	1	BA87566_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Esin_And2018_Cyb	D10	Cytb	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	ACCCCTCCTCATATCCAACCA	130.87402
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA87566_Mjap_Gib2022_COI	D10	1	BA87566_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Mjap_Gib2022_COI	D10	COI	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	AATTTAATTGTGACTAGGCATGGTCTG	130.87402
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA87567_Hsan_Wil2021_COI	D11	1	BA87567_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Hsan_Wil2021_COI	D11	COI	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	CCTGGGCCGGTATAGTAGGT	130.87453
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA87567_Mros_Gib2022_COI	D11	1	BA87567_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Mros_Gib2022_COI	D11	COI	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87453
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA87567_Pvir_Dia2013_COI	D11	1	BA87567_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Pvir_Dia2013_COI	D11	COI	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	CTTAGTGGCATTAATTCGDAATCC	130.87453
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA87567_Rhar_Sim2018_COI	D11	1	BA87567_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Rhar_Sim2018_COI	D11	COI	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	CTGCTGCTATTGCTCATG	130.87453
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA87569_Pper_Dia2013_COI	D22	1	BA87569_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Pper_Dia2013_COI	D22	COI	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	CTTAGTGGCATTAATTCGDAATCC	130.82694
BA92914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77559	WGS84	BA92914	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.273	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92914_Cmae_Bot2011_COI	NP17	1	BA92914_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92914	Sample	BA92914	targeted	Present	Sample	Cmae_Bot2011_COI	NP17	COI	45	BA92914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP17	ATGAACAGTCTATCCTCCTTTAG	138.48343
BA92916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77468	WGS84	BA92916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.20358658	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92916_Sspa_Oph2007_COI	NP3	1	BA92916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92916	Sample	BA92916	targeted	Present	Sample	Sspa_Oph2007_COI	NP3	COI	45	BA92916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP3	GAAGGCCTTCCGATGATC	138.48385
BA92925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77622	WGS84	BA92925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.69	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92925_Cmae_Bot2011_COI	NP4	1	BA92925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92925	Sample	BA92925	targeted	Present	Sample	Cmae_Bot2011_COI	NP4	COI	45	BA92925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP4	ATGAACAGTCTATCCTCCTTTAG	138.48279
BA92934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.7746	WGS84	BA92934	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.42347717	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92934_Sspa_Oph2007_COI	NP10	1	BA92934_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92934	Sample	BA92934	targeted	Present	Sample	Sspa_Oph2007_COI	NP10	COI	45	BA92934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP10	GAAGGCCTTCCGATGATC	138.48406
BA92936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77468	WGS84	BA92936	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.626	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92936_Cmae_Bot2011_COI	NP11	1	BA92936_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92936	Sample	BA92936	targeted	Present	Sample	Cmae_Bot2011_COI	NP11	COI	45	BA92936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP11	ATGAACAGTCTATCCTCCTTTAG	138.484
BA92949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77618	WGS84	BA92949	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.78789139	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92949_Sspa_Oph2007_COI	NP15	1	BA92949_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92949	Sample	BA92949	targeted	Present	Sample	Sspa_Oph2007_COI	NP15	COI	45	BA92949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP15	GAAGGCCTTCCGATGATC	138.48305
BA99030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84208	WGS84	BA99030	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.08	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99030_Aamu_Bax2006_COI	M18	1	BA99030_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99030	Sample	BA99030	targeted	Present	Sample	Aamu_Bax2006_COI	M18	COI	45	BA99030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M18	GCACAACCGGGATCTTTACTTC	144.92133
BA99034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.85072	WGS84	BA99034	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.511	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99034_Aamu_Bax2006_COI	M4	1	BA99034_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99034	Sample	BA99034	targeted	Present	Sample	Aamu_Bax2006_COI	M4	COI	45	BA99034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M4	GCACAACCGGGATCTTTACTTC	144.90769
BA99034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.85072	WGS84	BA99034	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.09700012	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA99034_Sspa_Oph2007_COI	M4	1	BA99034_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99034	Sample	BA99034	targeted	Present	Sample	Sspa_Oph2007_COI	M4	COI	45	BA99034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M4	GAAGGCCTTCCGATGATC	144.90769
BA99034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.85072	WGS84	BA99034	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.573	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA99034_Upin_Bot2011_COI	M4	1	BA99034_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99034	Sample	BA99034	targeted	Present	Sample	Upin_Bot2011_COI	M4	COI	45	BA99034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M4	CTTTAATTACAGCGTTTTTATTGTTGT	144.90769
BA99036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.81933	WGS84	BA99036	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.237	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA99036_Cmae_Bot2011_COI	M6	1	BA99036_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99036	Sample	BA99036	targeted	Present	Sample	Cmae_Bot2011_COI	M6	COI	45	BA99036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M6	ATGAACAGTCTATCCTCCTTTAG	144.91226
BA99038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.8415	WGS84	BA99038	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.051	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99038_Aamu_Bax2006_COI	M11	1	BA99038_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99038	Sample	BA99038	targeted	Present	Sample	Aamu_Bax2006_COI	M11	COI	45	BA99038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M11	GCACAACCGGGATCTTTACTTC	144.92958
BA99041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.8459	WGS84	BA99041	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.755	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99041_Aamu_Bax2006_COI	M23	1	BA99041_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99041	Sample	BA99041	targeted	Present	Sample	Aamu_Bax2006_COI	M23	COI	45	BA99041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M23	GCACAACCGGGATCTTTACTTC	144.92684
BB10182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81876	WGS84	BB10182	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.722	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10182_Cmae_Bot2011_COI	AS1	1	BB10182_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10182	Sample	BB10182	targeted	Present	Sample	Cmae_Bot2011_COI	AS1	COI	45	BB10182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS1	ATGAACAGTCTATCCTCCTTTAG	138.51161
BB10186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81637	WGS84	BB10186	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.213	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10186_Cmae_Bot2011_COI	AS5	1	BB10186_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10186	Sample	BB10186	targeted	Present	Sample	Cmae_Bot2011_COI	AS5	COI	45	BB10186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS5	ATGAACAGTCTATCCTCCTTTAG	138.5126
BB10193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.80906	WGS84	BB10193	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.95	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10193_Cmae_Bot2011_COI	AS12	1	BB10193_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10193	Sample	BB10193	targeted	Present	Sample	Cmae_Bot2011_COI	AS12	COI	45	BB10193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS12	ATGAACAGTCTATCCTCCTTTAG	138.5159
BB10197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81002	WGS84	BB10197	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.269	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10197_Cmae_Bot2011_COI	AS16	1	BB10197_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10197	Sample	BB10197	targeted	Present	Sample	Cmae_Bot2011_COI	AS16	COI	45	BB10197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS16	ATGAACAGTCTATCCTCCTTTAG	138.51966
BB10201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.8295	WGS84	BB10201	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.557	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10201_Cmae_Bot2011_COI	AS20	1	BB10201_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10201	Sample	BB10201	targeted	Present	Sample	Cmae_Bot2011_COI	AS20	COI	45	BB10201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS20	ATGAACAGTCTATCCTCCTTTAG	138.50707
BB10205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.8106	WGS84	BB10205	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.501	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10205_Cmae_Bot2011_COI	AS24	1	BB10205_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10205	Sample	BB10205	targeted	Present	Sample	Cmae_Bot2011_COI	AS24	COI	45	BB10205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS24	ATGAACAGTCTATCCTCCTTTAG	138.51439
BB12679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88398	WGS84	BB12679	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.199	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12679_Mgig_Bot2012_COI	H37	1	BB12679_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12679	Sample	BB12679	targeted	Present	Sample	Mgig_Bot2012_COI	H37	COI	45	BB12679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H37	TCTTATTCGTTGGAGACTTTATAACCCT	147.33726
BB12681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88622	WGS84	BB12681	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.698	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB12681_Aamu_Bax2006_COI	H39	1	BB12681_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12681	Sample	BB12681	targeted	Present	Sample	Aamu_Bax2006_COI	H39	COI	45	BB12681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H39	GCACAACCGGGATCTTTACTTC	147.33941
BB12682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.84406	WGS84	BB12682	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.785	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12682_Mgig_Bot2012_COI	H40	1	BB12682_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12682	Sample	BB12682	targeted	Present	Sample	Mgig_Bot2012_COI	H40	COI	45	BB12682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H40	TCTTATTCGTTGGAGACTTTATAACCCT	147.33059
BB12683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87437	WGS84	BB12683	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.264	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12683_Cmae_Bot2011_COI	H41	1	BB12683_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12683	Sample	BB12683	targeted	Present	Sample	Cmae_Bot2011_COI	H41	COI	45	BB12683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H41	ATGAACAGTCTATCCTCCTTTAG	147.33773
BB12685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88086	WGS84	BB12685	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.298	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB12685_Upin_Bot2011_COI	H43	1	BB12685_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12685	Sample	BB12685	targeted	Present	Sample	Upin_Bot2011_COI	H43	COI	45	BB12685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H43	CTTTAATTACAGCGTTTTTATTGTTGT	147.34152
BB12686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88485	WGS84	BB12686	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	21.253	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12686_Mgig_Bot2012_COI	H44	1	BB12686_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12686	Sample	BB12686	targeted	Present	Sample	Mgig_Bot2012_COI	H44	COI	45	BB12686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H44	TCTTATTCGTTGGAGACTTTATAACCCT	147.33488
BB12687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88542	WGS84	BB12687	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.344	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12687_Cmae_Bot2011_COI	H45	1	BB12687_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12687	Sample	BB12687	targeted	Present	Sample	Cmae_Bot2011_COI	H45	COI	45	BB12687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H45	ATGAACAGTCTATCCTCCTTTAG	147.33407
BB12690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.83967	WGS84	BB12690	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.169	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12690_Cmae_Bot2011_COI	H48	1	BB12690_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12690	Sample	BB12690	targeted	Present	Sample	Cmae_Bot2011_COI	H48	COI	45	BB12690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H48	ATGAACAGTCTATCCTCCTTTAG	147.34096
BB12693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.82529	WGS84	BB12693	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.361	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12693_Mgig_Bot2012_COI	H51	1	BB12693_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12693	Sample	BB12693	targeted	Present	Sample	Mgig_Bot2012_COI	H51	COI	45	BB12693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H51	TCTTATTCGTTGGAGACTTTATAACCCT	147.30513
BB12694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87855	WGS84	BB12694	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.215	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12694_Cmae_Bot2011_COI	H52	1	BB12694_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12694	Sample	BB12694	targeted	Present	Sample	Cmae_Bot2011_COI	H52	COI	45	BB12694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H52	ATGAACAGTCTATCCTCCTTTAG	147.34145
BB17782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84053	WGS84	BB17782	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.028	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17782_Mgig_Bot2012_COI	S39	1	BB17782_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17782	Sample	BB17782	targeted	Present	Sample	Mgig_Bot2012_COI	S39	COI	45	BB17782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S39	TCTTATTCGTTGGAGACTTTATAACCCT	151.25412
BB17786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84465	WGS84	BB17786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.017	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17786_Mgig_Bot2012_COI	S43	1	BB17786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17786	Sample	BB17786	targeted	Present	Sample	Mgig_Bot2012_COI	S43	COI	45	BB17786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S43	TCTTATTCGTTGGAGACTTTATAACCCT	151.278
BB17793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84793	WGS84	BB17793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.198	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17793_Mgig_Bot2012_COI	S50	1	BB17793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17793	Sample	BB17793	targeted	Present	Sample	Mgig_Bot2012_COI	S50	COI	45	BB17793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S50	TCTTATTCGTTGGAGACTTTATAACCCT	151.2731
BB17797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84405	WGS84	BB17797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.592	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17797_Mgig_Bot2012_COI	S54	1	BB17797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17797	Sample	BB17797	targeted	Present	Sample	Mgig_Bot2012_COI	S54	COI	45	BB17797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S54	TCTTATTCGTTGGAGACTTTATAACCCT	151.27853
BB32902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01817	WGS84	BB32902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32902_Cmae_Bot2011_COI	PS-A01	1	BB32902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32902	Sample	BB32902	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A01	COI	45	BB32902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A01	ATGAACAGTCTATCCTCCTTTAG	137.60078
BB32902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01817	WGS84	BB32902	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32902_Msal_Bot2012_COI	PS-A01	1	BB32902_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32902	Sample	BB32902	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A01	COI	45	BB32902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A01	GYTAGTTCCRATGATGTTAGCTG	137.60078
BB32904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01922	WGS84	BB32904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32904_Aamu_Bax2006_COI	PS-A03	1	BB32904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32904	Sample	BB32904	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A03	COI	45	BB32904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A03	GCACAACCGGGATCTTTACTTC	137.59862
BB32904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01922	WGS84	BB32904	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32904_Pcan_Bot2011_IGS	PS-A03	1	BB32904_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32904	Sample	BB32904	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A03	IGS	45	BB32904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A03	CGTAATCCTCAGTACTGGCTA	137.59862
BB32904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01922	WGS84	BB32904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.12700081	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32904_Sspa_Oph2007_COI	PS-A03	1	BB32904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32904	Sample	BB32904	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A03	COI	45	BB32904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A03	GAAGGCCTTCCGATGATC	137.59862
BB32904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01922	WGS84	BB32904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32904_Upin_Bot2011_COI	PS-A03	1	BB32904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32904	Sample	BB32904	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A03	COI	45	BB32904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A03	CTTTAATTACAGCGTTTTTATTGTTGT	137.59862
BB32905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02878	WGS84	BB32905	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32905_Mgig_Bot2012_COI	PS-A04	1	BB32905_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32905	Sample	BB32905	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A04	COI	45	BB32905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A04	TCTTATTCGTTGGAGACTTTATAACCCT	137.60044
BB32906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03257	WGS84	BB32906	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32906_Cmae_Bot2011_COI	PS-A05	1	BB32906_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32906	Sample	BB32906	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A05	COI	45	BB32906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A05	ATGAACAGTCTATCCTCCTTTAG	137.60012
BB32906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03257	WGS84	BB32906	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32906_Msal_Bot2012_COI	PS-A05	1	BB32906_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32906	Sample	BB32906	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A05	COI	45	BB32906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A05	GYTAGTTCCRATGATGTTAGCTG	137.60012
BB32908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03939	WGS84	BB32908	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32908_Aamu_Bax2006_COI	PS-A07	1	BB32908_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32908	Sample	BB32908	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A07	COI	45	BB32908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A07	GCACAACCGGGATCTTTACTTC	137.59628
BB32908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03939	WGS84	BB32908	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32908_Pcan_Bot2011_IGS	PS-A07	1	BB32908_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32908	Sample	BB32908	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A07	IGS	45	BB32908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A07	CGTAATCCTCAGTACTGGCTA	137.59628
BB32908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03939	WGS84	BB32908	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.63600159	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32908_Sspa_Oph2007_COI	PS-A07	1	BB32908_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32908	Sample	BB32908	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A07	COI	45	BB32908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A07	GAAGGCCTTCCGATGATC	137.59628
BB32908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03939	WGS84	BB32908	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32908_Upin_Bot2011_COI	PS-A07	1	BB32908_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32908	Sample	BB32908	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A07	COI	45	BB32908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A07	CTTTAATTACAGCGTTTTTATTGTTGT	137.59628
BB32909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04109	WGS84	BB32909	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32909_Mgig_Bot2012_COI	PS-A08	1	BB32909_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32909	Sample	BB32909	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A08	COI	45	BB32909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A08	TCTTATTCGTTGGAGACTTTATAACCCT	137.59478
BB32911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03909	WGS84	BB32911	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32911_Aamu_Bax2006_COI	PS-A10	1	BB32911_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32911	Sample	BB32911	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A10	COI	45	BB32911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A10	GCACAACCGGGATCTTTACTTC	137.59297
BB32911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03909	WGS84	BB32911	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32911_Pcan_Bot2011_IGS	PS-A10	1	BB32911_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32911	Sample	BB32911	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A10	IGS	45	BB32911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A10	CGTAATCCTCAGTACTGGCTA	137.59297
BB32911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03909	WGS84	BB32911	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.44599915	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32911_Sspa_Oph2007_COI	PS-A10	1	BB32911_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32911	Sample	BB32911	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A10	COI	45	BB32911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A10	GAAGGCCTTCCGATGATC	137.59297
BB32911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03909	WGS84	BB32911	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32911_Upin_Bot2011_COI	PS-A10	1	BB32911_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32911	Sample	BB32911	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A10	COI	45	BB32911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A10	CTTTAATTACAGCGTTTTTATTGTTGT	137.59297
BB32912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01736	WGS84	BB32912	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32912_Mgig_Bot2012_COI	PS-A11	1	BB32912_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32912	Sample	BB32912	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A11	COI	45	BB32912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A11	TCTTATTCGTTGGAGACTTTATAACCCT	137.60109
BB32913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01847	WGS84	BB32913	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32913_Cmae_Bot2011_COI	PS-A12	1	BB32913_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32913	Sample	BB32913	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A12	COI	45	BB32913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A12	ATGAACAGTCTATCCTCCTTTAG	137.59956
BB32913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01847	WGS84	BB32913	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32913_Msal_Bot2012_COI	PS-A12	1	BB32913_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32913	Sample	BB32913	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A12	COI	45	BB32913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A12	GYTAGTTCCRATGATGTTAGCTG	137.59956
BB32915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01772	WGS84	BB32915	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32915_Aamu_Bax2006_COI	PS-A14	1	BB32915_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32915	Sample	BB32915	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A14	COI	45	BB32915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A14	GCACAACCGGGATCTTTACTTC	137.59601
BB32915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01772	WGS84	BB32915	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32915_Pcan_Bot2011_IGS	PS-A14	1	BB32915_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32915	Sample	BB32915	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A14	IGS	45	BB32915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A14	CGTAATCCTCAGTACTGGCTA	137.59601
BB32915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01772	WGS84	BB32915	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.62599945	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32915_Sspa_Oph2007_COI	PS-A14	1	BB32915_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32915	Sample	BB32915	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A14	COI	45	BB32915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A14	GAAGGCCTTCCGATGATC	137.59601
BB32915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01772	WGS84	BB32915	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32915_Upin_Bot2011_COI	PS-A14	1	BB32915_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32915	Sample	BB32915	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A14	COI	45	BB32915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A14	CTTTAATTACAGCGTTTTTATTGTTGT	137.59601
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.01845	WGS84	BB32916	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32916_Asen_Wil2025_COI	PS-A15	1	BB32916_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	GTTGTTGTTACAACACATGCTCTTTTG	137.59697
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01845	WGS84	BB32916	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32916_Mgig_Bot2012_COI	PS-A15	1	BB32916_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	TCTTATTCGTTGGAGACTTTATAACCCT	137.59697
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01982	WGS84	BB32917	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32917_Cmae_Bot2011_COI	PS-A16	1	BB32917_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	ATGAACAGTCTATCCTCCTTTAG	137.59981
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01982	WGS84	BB32917	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32917_Msal_Bot2012_COI	PS-A16	1	BB32917_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	GYTAGTTCCRATGATGTTAGCTG	137.59981
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02985	WGS84	BB32919	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32919_Aamu_Bax2006_COI	PS-A18	1	BB32919_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	GCACAACCGGGATCTTTACTTC	137.60018
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02985	WGS84	BB32919	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32919_Pcan_Bot2011_IGS	PS-A18	1	BB32919_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A18	IGS	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	CGTAATCCTCAGTACTGGCTA	137.60018
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02985	WGS84	BB32919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.09899902	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32919_Sspa_Oph2007_COI	PS-A18	1	BB32919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	GAAGGCCTTCCGATGATC	137.60018
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02985	WGS84	BB32919	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32919_Upin_Bot2011_COI	PS-A18	1	BB32919_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	CTTTAATTACAGCGTTTTTATTGTTGT	137.60018
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.02985	WGS84	BB32919	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32919_Vgib_Wil2025_COI	PS-A18	1	BB32919_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	CCCCTCTTTACAAGTTCTTTAGGGT	137.60018
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03113	WGS84	BB32920	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32920_Cmae_Bot2011_COI	PS-A19	1	BB32920_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	ATGAACAGTCTATCCTCCTTTAG	137.60008
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03113	WGS84	BB32920	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32920_Msal_Bot2012_COI	PS-A19	1	BB32920_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	GYTAGTTCCRATGATGTTAGCTG	137.60008
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03692	WGS84	BB32922	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32922_Aamu_Bax2006_COI	PS-A21	1	BB32922_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	GCACAACCGGGATCTTTACTTC	137.59936
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03692	WGS84	BB32922	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32922_Pcan_Bot2011_IGS	PS-A21	1	BB32922_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A21	IGS	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	CGTAATCCTCAGTACTGGCTA	137.59936
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03692	WGS84	BB32922	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.61400032	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32922_Sspa_Oph2007_COI	PS-A21	1	BB32922_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	GAAGGCCTTCCGATGATC	137.59936
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03692	WGS84	BB32922	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32922_Upin_Bot2011_COI	PS-A21	1	BB32922_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	CTTTAATTACAGCGTTTTTATTGTTGT	137.59936
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03692	WGS84	BB32922	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32922_Vgib_Wil2025_COI	PS-A21	1	BB32922_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	CCCCTCTTTACAAGTTCTTTAGGGT	137.59936
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03696	WGS84	BB32923	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32923_Asen_Wil2025_COI	PS-A22	1	BB32923_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	GTTGTTGTTACAACACATGCTCTTTTG	137.59708
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03696	WGS84	BB32923	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32923_Mgig_Bot2012_COI	PS-A22	1	BB32923_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	TCTTATTCGTTGGAGACTTTATAACCCT	137.59708
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03825	WGS84	BB32924	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32924_Cmae_Bot2011_COI	PS-A23	1	BB32924_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	ATGAACAGTCTATCCTCCTTTAG	137.59652
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03825	WGS84	BB32924	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32924_Msal_Bot2012_COI	PS-A23	1	BB32924_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	GYTAGTTCCRATGATGTTAGCTG	137.59652
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.0418	WGS84	BB32926	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32926_Aamu_Bax2006_COI	PS-A25	1	BB32926_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	GCACAACCGGGATCTTTACTTC	137.59386
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.0418	WGS84	BB32926	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32926_Pcan_Bot2011_IGS	PS-A25	1	BB32926_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A25	IGS	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	CGTAATCCTCAGTACTGGCTA	137.59386
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.0418	WGS84	BB32926	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.66399956	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32926_Sspa_Oph2007_COI	PS-A25	1	BB32926_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	GAAGGCCTTCCGATGATC	137.59386
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.0418	WGS84	BB32926	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32926_Upin_Bot2011_COI	PS-A25	1	BB32926_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	CTTTAATTACAGCGTTTTTATTGTTGT	137.59386
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.0418	WGS84	BB32926	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32926_Vgib_Wil2025_COI	PS-A25	1	BB32926_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	CCCCTCTTTACAAGTTCTTTAGGGT	137.59386
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04086	WGS84	BB32927	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32927_Asen_Wil2025_COI	PS-A26	1	BB32927_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	GTTGTTGTTACAACACATGCTCTTTTG	137.59588
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04086	WGS84	BB32927	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32927_Mgig_Bot2012_COI	PS-A26	1	BB32927_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	TCTTATTCGTTGGAGACTTTATAACCCT	137.59588
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03928	WGS84	BB32928	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32928_Cmae_Bot2011_COI	PS-A27	1	BB32928_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	ATGAACAGTCTATCCTCCTTTAG	137.59475
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03928	WGS84	BB32928	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32928_Msal_Bot2012_COI	PS-A27	1	BB32928_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	GYTAGTTCCRATGATGTTAGCTG	137.59475
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03918	WGS84	BB32930	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32930_Asen_Wil2025_COI	PS-A29	1	BB32930_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	GTTGTTGTTACAACACATGCTCTTTTG	137.59354
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03918	WGS84	BB32930	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32930_Mgig_Bot2012_COI	PS-A29	1	BB32930_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	TCTTATTCGTTGGAGACTTTATAACCCT	137.59354
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04053	WGS84	BB32931	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32931_Cmae_Bot2011_COI	PS-A30	1	BB32931_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	ATGAACAGTCTATCCTCCTTTAG	137.59253
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04053	WGS84	BB32931	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32931_Msal_Bot2012_COI	PS-A30	1	BB32931_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	GYTAGTTCCRATGATGTTAGCTG	137.59253
BB32937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71952	WGS84	BB32937	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32937_Aamu_Bax2006_COI	PS-B01	1	BB32937_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32937	Sample	BB32937	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B01	COI	45	BB32937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B01	GCACAACCGGGATCTTTACTTC	135.86182
BB32937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71952	WGS84	BB32937	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32937_Pcan_Bot2011_IGS	PS-B01	1	BB32937_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32937	Sample	BB32937	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B01	IGS	45	BB32937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B01	CGTAATCCTCAGTACTGGCTA	135.86182
BB32937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71952	WGS84	BB32937	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32937_Sspa_Oph2007_COI	PS-B01	1	BB32937_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32937	Sample	BB32937	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B01	COI	45	BB32937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B01	GAAGGCCTTCCGATGATC	135.86182
BB32937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71952	WGS84	BB32937	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32937_Upin_Bot2011_COI	PS-B01	1	BB32937_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32937	Sample	BB32937	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B01	COI	45	BB32937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B01	CTTTAATTACAGCGTTTTTATTGTTGT	135.86182
BB32938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72023	WGS84	BB32938	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32938_Mgig_Bot2012_COI	PS-B02	1	BB32938_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32938	Sample	BB32938	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B02	COI	45	BB32938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B02	TCTTATTCGTTGGAGACTTTATAACCCT	135.86471
BB32939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71791	WGS84	BB32939	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32939_Cmae_Bot2011_COI	PS-B03	1	BB32939_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32939	Sample	BB32939	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B03	COI	45	BB32939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B03	ATGAACAGTCTATCCTCCTTTAG	135.86262
BB32939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71791	WGS84	BB32939	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32939_Msal_Bot2012_COI	PS-B03	1	BB32939_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32939	Sample	BB32939	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B03	COI	45	BB32939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B03	GYTAGTTCCRATGATGTTAGCTG	135.86262
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71539	WGS84	BB32940	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32940_Aamu_Bax2006_COI	PS-B04	1	BB32940_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	GCACAACCGGGATCTTTACTTC	135.86716
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71539	WGS84	BB32940	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32940_Pcan_Bot2011_IGS	PS-B04	1	BB32940_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B04	IGS	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	CGTAATCCTCAGTACTGGCTA	135.86716
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71539	WGS84	BB32940	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32940_Sspa_Oph2007_COI	PS-B04	1	BB32940_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	GAAGGCCTTCCGATGATC	135.86716
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71539	WGS84	BB32940	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32940_Upin_Bot2011_COI	PS-B04	1	BB32940_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	CTTTAATTACAGCGTTTTTATTGTTGT	135.86716
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71539	WGS84	BB32940	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32940_Vgib_Wil2025_COI	PS-B04	1	BB32940_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	CCCCTCTTTACAAGTTCTTTAGGGT	135.86716
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71512	WGS84	BB32941	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32941_Asen_Wil2025_COI	PS-B05	1	BB32941_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	GTTGTTGTTACAACACATGCTCTTTTG	135.87171
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71512	WGS84	BB32941	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32941_Mgig_Bot2012_COI	PS-B05	1	BB32941_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	TCTTATTCGTTGGAGACTTTATAACCCT	135.87171
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71875	WGS84	BB32942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32942_Cmae_Bot2011_COI	PS-B06	1	BB32942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	ATGAACAGTCTATCCTCCTTTAG	135.87153
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71875	WGS84	BB32942	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32942_Msal_Bot2012_COI	PS-B06	1	BB32942_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	GYTAGTTCCRATGATGTTAGCTG	135.87153
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71838	WGS84	BB32944	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32944_Aamu_Bax2006_COI	PS-B08	1	BB32944_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	GCACAACCGGGATCTTTACTTC	135.87715
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71838	WGS84	BB32944	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32944_Pcan_Bot2011_IGS	PS-B08	1	BB32944_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B08	IGS	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	CGTAATCCTCAGTACTGGCTA	135.87715
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71838	WGS84	BB32944	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32944_Sspa_Oph2007_COI	PS-B08	1	BB32944_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	GAAGGCCTTCCGATGATC	135.87715
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71838	WGS84	BB32944	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32944_Upin_Bot2011_COI	PS-B08	1	BB32944_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	CTTTAATTACAGCGTTTTTATTGTTGT	135.87715
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71838	WGS84	BB32944	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32944_Vgib_Wil2025_COI	PS-B08	1	BB32944_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	CCCCTCTTTACAAGTTCTTTAGGGT	135.87715
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.72015	WGS84	BB32945	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32945_Asen_Wil2025_COI	PS-B09	1	BB32945_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	GTTGTTGTTACAACACATGCTCTTTTG	135.8806
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72015	WGS84	BB32945	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32945_Mgig_Bot2012_COI	PS-B09	1	BB32945_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	TCTTATTCGTTGGAGACTTTATAACCCT	135.8806
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72483	WGS84	BB32946	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32946_Cmae_Bot2011_COI	PS-B10	1	BB32946_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	ATGAACAGTCTATCCTCCTTTAG	135.88569
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72483	WGS84	BB32946	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32946_Msal_Bot2012_COI	PS-B10	1	BB32946_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	GYTAGTTCCRATGATGTTAGCTG	135.88569
BB32948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72021	WGS84	BB32948	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32948_Aamu_Bax2006_COI	PS-B12	1	BB32948_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32948	Sample	BB32948	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B12	COI	45	BB32948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B12	GCACAACCGGGATCTTTACTTC	135.8631
BB32948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72021	WGS84	BB32948	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32948_Pcan_Bot2011_IGS	PS-B12	1	BB32948_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32948	Sample	BB32948	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B12	IGS	45	BB32948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B12	CGTAATCCTCAGTACTGGCTA	135.8631
BB32948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72021	WGS84	BB32948	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.51499939	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32948_Sspa_Oph2007_COI	PS-B12	1	BB32948_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32948	Sample	BB32948	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B12	COI	45	BB32948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B12	GAAGGCCTTCCGATGATC	135.8631
BB32948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72021	WGS84	BB32948	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32948_Upin_Bot2011_COI	PS-B12	1	BB32948_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32948	Sample	BB32948	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B12	COI	45	BB32948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B12	CTTTAATTACAGCGTTTTTATTGTTGT	135.8631
BB32949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72033	WGS84	BB32949	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32949_Mgig_Bot2012_COI	PS-B13	1	BB32949_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32949	Sample	BB32949	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B13	COI	45	BB32949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B13	TCTTATTCGTTGGAGACTTTATAACCCT	135.86433
BB32951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71958	WGS84	BB32951	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32951_Aamu_Bax2006_COI	PS-B15	1	BB32951_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32951	Sample	BB32951	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B15	COI	45	BB32951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B15	GCACAACCGGGATCTTTACTTC	135.86192
BB32951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71958	WGS84	BB32951	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32951_Pcan_Bot2011_IGS	PS-B15	1	BB32951_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32951	Sample	BB32951	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B15	IGS	45	BB32951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B15	CGTAATCCTCAGTACTGGCTA	135.86192
BB32951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71958	WGS84	BB32951	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.95299911	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32951_Sspa_Oph2007_COI	PS-B15	1	BB32951_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32951	Sample	BB32951	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B15	COI	45	BB32951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B15	GAAGGCCTTCCGATGATC	135.86192
BB32951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71958	WGS84	BB32951	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32951_Upin_Bot2011_COI	PS-B15	1	BB32951_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32951	Sample	BB32951	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B15	COI	45	BB32951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B15	CTTTAATTACAGCGTTTTTATTGTTGT	135.86192
BB32952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71716	WGS84	BB32952	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32952_Mgig_Bot2012_COI	PS-B16	1	BB32952_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32952	Sample	BB32952	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B16	COI	45	BB32952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B16	TCTTATTCGTTGGAGACTTTATAACCCT	135.8641
BB32953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7164	WGS84	BB32953	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32953_Cmae_Bot2011_COI	PS-B17	1	BB32953_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32953	Sample	BB32953	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B17	COI	45	BB32953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B17	ATGAACAGTCTATCCTCCTTTAG	135.86547
BB32953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7164	WGS84	BB32953	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32953_Msal_Bot2012_COI	PS-B17	1	BB32953_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32953	Sample	BB32953	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B17	COI	45	BB32953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B17	GYTAGTTCCRATGATGTTAGCTG	135.86547
BB32955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71395	WGS84	BB32955	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32955_Aamu_Bax2006_COI	PS-B19	1	BB32955_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32955	Sample	BB32955	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B19	COI	45	BB32955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B19	GCACAACCGGGATCTTTACTTC	135.87171
BB32955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71395	WGS84	BB32955	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32955_Pcan_Bot2011_IGS	PS-B19	1	BB32955_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32955	Sample	BB32955	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B19	IGS	45	BB32955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B19	CGTAATCCTCAGTACTGGCTA	135.87171
BB32955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71395	WGS84	BB32955	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.63299942	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32955_Sspa_Oph2007_COI	PS-B19	1	BB32955_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32955	Sample	BB32955	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B19	COI	45	BB32955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B19	GAAGGCCTTCCGATGATC	135.87171
BB32955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71395	WGS84	BB32955	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32955_Upin_Bot2011_COI	PS-B19	1	BB32955_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32955	Sample	BB32955	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B19	COI	45	BB32955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B19	CTTTAATTACAGCGTTTTTATTGTTGT	135.87171
BB32956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71632	WGS84	BB32956	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32956_Mgig_Bot2012_COI	PS-B20	1	BB32956_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32956	Sample	BB32956	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B20	COI	45	BB32956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B20	TCTTATTCGTTGGAGACTTTATAACCCT	135.87171
BB32957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7175	WGS84	BB32957	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32957_Cmae_Bot2011_COI	PS-B21	1	BB32957_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32957	Sample	BB32957	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B21	COI	45	BB32957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B21	ATGAACAGTCTATCCTCCTTTAG	135.87182
BB32957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7175	WGS84	BB32957	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32957_Msal_Bot2012_COI	PS-B21	1	BB32957_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32957	Sample	BB32957	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B21	COI	45	BB32957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B21	GYTAGTTCCRATGATGTTAGCTG	135.87182
BB32959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72081	WGS84	BB32959	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32959_Aamu_Bax2006_COI	PS-B23	1	BB32959_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32959	Sample	BB32959	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B23	COI	45	BB32959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B23	GCACAACCGGGATCTTTACTTC	135.87177
BB32959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72081	WGS84	BB32959	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32959_Pcan_Bot2011_IGS	PS-B23	1	BB32959_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32959	Sample	BB32959	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B23	IGS	45	BB32959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B23	CGTAATCCTCAGTACTGGCTA	135.87177
BB32959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72081	WGS84	BB32959	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32959_Sspa_Oph2007_COI	PS-B23	1	BB32959_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32959	Sample	BB32959	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B23	COI	45	BB32959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B23	GAAGGCCTTCCGATGATC	135.87177
BB32959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72081	WGS84	BB32959	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32959_Upin_Bot2011_COI	PS-B23	1	BB32959_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32959	Sample	BB32959	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B23	COI	45	BB32959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B23	CTTTAATTACAGCGTTTTTATTGTTGT	135.87177
BB32960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71918	WGS84	BB32960	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32960_Cmae_Bot2011_COI	PS-B24	1	BB32960_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32960	Sample	BB32960	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B24	COI	45	BB32960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B24	ATGAACAGTCTATCCTCCTTTAG	135.87416
BB32960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71918	WGS84	BB32960	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32960_Msal_Bot2012_COI	PS-B24	1	BB32960_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32960	Sample	BB32960	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B24	COI	45	BB32960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B24	GYTAGTTCCRATGATGTTAGCTG	135.87416
BB32962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71858	WGS84	BB32962	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32962_Aamu_Bax2006_COI	PS-B26	1	BB32962_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32962	Sample	BB32962	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B26	COI	45	BB32962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B26	GCACAACCGGGATCTTTACTTC	135.87847
BB32962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71858	WGS84	BB32962	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32962_Pcan_Bot2011_IGS	PS-B26	1	BB32962_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32962	Sample	BB32962	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B26	IGS	45	BB32962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B26	CGTAATCCTCAGTACTGGCTA	135.87847
BB32962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71858	WGS84	BB32962	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.56100082	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32962_Sspa_Oph2007_COI	PS-B26	1	BB32962_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32962	Sample	BB32962	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B26	COI	45	BB32962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B26	GAAGGCCTTCCGATGATC	135.87847
BB32962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71858	WGS84	BB32962	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32962_Upin_Bot2011_COI	PS-B26	1	BB32962_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32962	Sample	BB32962	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B26	COI	45	BB32962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B26	CTTTAATTACAGCGTTTTTATTGTTGT	135.87847
BB32963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71932	WGS84	BB32963	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32963_Mgig_Bot2012_COI	PS-B27	1	BB32963_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32963	Sample	BB32963	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B27	COI	45	BB32963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B27	TCTTATTCGTTGGAGACTTTATAACCCT	135.87964
BB32964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.723	WGS84	BB32964	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32964_Cmae_Bot2011_COI	PS-B28	1	BB32964_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32964	Sample	BB32964	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B28	COI	45	BB32964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B28	ATGAACAGTCTATCCTCCTTTAG	135.88382
BB32964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.723	WGS84	BB32964	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32964_Msal_Bot2012_COI	PS-B28	1	BB32964_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32964	Sample	BB32964	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B28	COI	45	BB32964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B28	GYTAGTTCCRATGATGTTAGCTG	135.88382
BB32966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72586	WGS84	BB32966	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32966_Aamu_Bax2006_COI	PS-B30	1	BB32966_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32966	Sample	BB32966	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B30	COI	45	BB32966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B30	GCACAACCGGGATCTTTACTTC	135.88634
BB32966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72586	WGS84	BB32966	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32966_Pcan_Bot2011_IGS	PS-B30	1	BB32966_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32966	Sample	BB32966	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B30	IGS	45	BB32966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B30	CGTAATCCTCAGTACTGGCTA	135.88634
BB32966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72586	WGS84	BB32966	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32966_Sspa_Oph2007_COI	PS-B30	1	BB32966_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32966	Sample	BB32966	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B30	COI	45	BB32966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B30	GAAGGCCTTCCGATGATC	135.88634
BB32966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72586	WGS84	BB32966	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32966_Upin_Bot2011_COI	PS-B30	1	BB32966_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32966	Sample	BB32966	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B30	COI	45	BB32966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B30	CTTTAATTACAGCGTTTTTATTGTTGT	135.88634
BB32973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93135	WGS84	BB32973	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32973_Aamu_Bax2006_COI	PS-C02	1	BB32973_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32973	Sample	BB32973	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C02	COI	45	BB32973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C02	GCACAACCGGGATCTTTACTTC	137.60781
BB32973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93135	WGS84	BB32973	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32973_Pcan_Bot2011_IGS	PS-C02	1	BB32973_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32973	Sample	BB32973	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C02	IGS	45	BB32973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C02	CGTAATCCTCAGTACTGGCTA	137.60781
BB32973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93135	WGS84	BB32973	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32973_Sspa_Oph2007_COI	PS-C02	1	BB32973_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32973	Sample	BB32973	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C02	COI	45	BB32973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C02	GAAGGCCTTCCGATGATC	137.60781
BB32973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93135	WGS84	BB32973	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32973_Upin_Bot2011_COI	PS-C02	1	BB32973_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32973	Sample	BB32973	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C02	COI	45	BB32973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C02	CTTTAATTACAGCGTTTTTATTGTTGT	137.60781
BB32974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92647	WGS84	BB32974	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32974_Mgig_Bot2012_COI	PS-C03	1	BB32974_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32974	Sample	BB32974	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C03	COI	45	BB32974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C03	TCTTATTCGTTGGAGACTTTATAACCCT	137.61272
BB32975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92373	WGS84	BB32975	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32975_Cmae_Bot2011_COI	PS-C04	1	BB32975_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32975	Sample	BB32975	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C04	COI	45	BB32975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C04	ATGAACAGTCTATCCTCCTTTAG	137.61315
BB32975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92373	WGS84	BB32975	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32975_Msal_Bot2012_COI	PS-C04	1	BB32975_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32975	Sample	BB32975	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C04	COI	45	BB32975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C04	GYTAGTTCCRATGATGTTAGCTG	137.61315
BB32977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92558	WGS84	BB32977	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32977_Aamu_Bax2006_COI	PS-C06	1	BB32977_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32977	Sample	BB32977	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C06	COI	45	BB32977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C06	GCACAACCGGGATCTTTACTTC	137.62398
BB32977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92558	WGS84	BB32977	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32977_Pcan_Bot2011_IGS	PS-C06	1	BB32977_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32977	Sample	BB32977	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C06	IGS	45	BB32977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C06	CGTAATCCTCAGTACTGGCTA	137.62398
BB32977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92558	WGS84	BB32977	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32977_Sspa_Oph2007_COI	PS-C06	1	BB32977_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32977	Sample	BB32977	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C06	COI	45	BB32977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C06	GAAGGCCTTCCGATGATC	137.62398
BB32977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92558	WGS84	BB32977	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32977_Upin_Bot2011_COI	PS-C06	1	BB32977_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32977	Sample	BB32977	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C06	COI	45	BB32977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C06	CTTTAATTACAGCGTTTTTATTGTTGT	137.62398
BB32978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92124	WGS84	BB32978	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32978_Mgig_Bot2012_COI	PS-C07	1	BB32978_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32978	Sample	BB32978	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C07	COI	45	BB32978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C07	TCTTATTCGTTGGAGACTTTATAACCCT	137.62582
BB32979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92229	WGS84	BB32979	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32979_Cmae_Bot2011_COI	PS-C08	1	BB32979_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32979	Sample	BB32979	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C08	COI	45	BB32979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C08	ATGAACAGTCTATCCTCCTTTAG	137.62767
BB32979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92229	WGS84	BB32979	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32979_Msal_Bot2012_COI	PS-C08	1	BB32979_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32979	Sample	BB32979	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C08	COI	45	BB32979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C08	GYTAGTTCCRATGATGTTAGCTG	137.62767
BB32980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92255	WGS84	BB32980	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32980_Aamu_Bax2006_COI	PS-C09	1	BB32980_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32980	Sample	BB32980	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C09	COI	45	BB32980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C09	GCACAACCGGGATCTTTACTTC	137.63506
BB32980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92255	WGS84	BB32980	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32980_Pcan_Bot2011_IGS	PS-C09	1	BB32980_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32980	Sample	BB32980	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C09	IGS	45	BB32980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C09	CGTAATCCTCAGTACTGGCTA	137.63506
BB32980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92255	WGS84	BB32980	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32980_Sspa_Oph2007_COI	PS-C09	1	BB32980_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32980	Sample	BB32980	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C09	COI	45	BB32980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C09	GAAGGCCTTCCGATGATC	137.63506
BB32980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92255	WGS84	BB32980	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32980_Upin_Bot2011_COI	PS-C09	1	BB32980_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32980	Sample	BB32980	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C09	COI	45	BB32980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C09	CTTTAATTACAGCGTTTTTATTGTTGT	137.63506
BB32981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92123	WGS84	BB32981	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32981_Mgig_Bot2012_COI	PS-C10	1	BB32981_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32981	Sample	BB32981	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C10	COI	45	BB32981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C10	TCTTATTCGTTGGAGACTTTATAACCCT	137.63952
BB32982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92987	WGS84	BB32982	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32982_Cmae_Bot2011_COI	PS-C11	1	BB32982_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32982	Sample	BB32982	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C11	COI	45	BB32982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C11	ATGAACAGTCTATCCTCCTTTAG	137.61215
BB32982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92987	WGS84	BB32982	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32982_Msal_Bot2012_COI	PS-C11	1	BB32982_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32982	Sample	BB32982	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C11	COI	45	BB32982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C11	GYTAGTTCCRATGATGTTAGCTG	137.61215
BB32984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93119	WGS84	BB32984	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32984_Aamu_Bax2006_COI	PS-C13	1	BB32984_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32984	Sample	BB32984	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C13	COI	45	BB32984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C13	GCACAACCGGGATCTTTACTTC	137.609
BB32984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93119	WGS84	BB32984	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32984_Pcan_Bot2011_IGS	PS-C13	1	BB32984_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32984	Sample	BB32984	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C13	IGS	45	BB32984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C13	CGTAATCCTCAGTACTGGCTA	137.609
BB32984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93119	WGS84	BB32984	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32984_Sspa_Oph2007_COI	PS-C13	1	BB32984_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32984	Sample	BB32984	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C13	COI	45	BB32984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C13	GAAGGCCTTCCGATGATC	137.609
BB32984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93119	WGS84	BB32984	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32984_Upin_Bot2011_COI	PS-C13	1	BB32984_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32984	Sample	BB32984	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C13	COI	45	BB32984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C13	CTTTAATTACAGCGTTTTTATTGTTGT	137.609
BB32985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93162	WGS84	BB32985	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32985_Mgig_Bot2012_COI	PS-C14	1	BB32985_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32985	Sample	BB32985	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C14	COI	45	BB32985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C14	TCTTATTCGTTGGAGACTTTATAACCCT	137.60666
BB32986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92686	WGS84	BB32986	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32986_Cmae_Bot2011_COI	PS-C15	1	BB32986_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32986	Sample	BB32986	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C15	COI	45	BB32986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C15	ATGAACAGTCTATCCTCCTTTAG	137.61371
BB32986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92686	WGS84	BB32986	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32986_Msal_Bot2012_COI	PS-C15	1	BB32986_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32986	Sample	BB32986	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C15	COI	45	BB32986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C15	GYTAGTTCCRATGATGTTAGCTG	137.61371
BB32988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92372	WGS84	BB32988	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32988_Aamu_Bax2006_COI	PS-C17	1	BB32988_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32988	Sample	BB32988	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C17	COI	45	BB32988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C17	GCACAACCGGGATCTTTACTTC	137.61164
BB32988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92372	WGS84	BB32988	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32988_Pcan_Bot2011_IGS	PS-C17	1	BB32988_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32988	Sample	BB32988	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C17	IGS	45	BB32988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C17	CGTAATCCTCAGTACTGGCTA	137.61164
BB32988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92372	WGS84	BB32988	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32988_Sspa_Oph2007_COI	PS-C17	1	BB32988_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32988	Sample	BB32988	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C17	COI	45	BB32988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C17	GAAGGCCTTCCGATGATC	137.61164
BB32988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92372	WGS84	BB32988	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32988_Upin_Bot2011_COI	PS-C17	1	BB32988_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32988	Sample	BB32988	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C17	COI	45	BB32988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C17	CTTTAATTACAGCGTTTTTATTGTTGT	137.61164
BB32989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92406	WGS84	BB32989	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32989_Mgig_Bot2012_COI	PS-C18	1	BB32989_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32989	Sample	BB32989	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C18	COI	45	BB32989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C18	TCTTATTCGTTGGAGACTTTATAACCCT	137.61464
BB32991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.9266	WGS84	BB32991	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32991_Aamu_Bax2006_COI	PS-C20	1	BB32991_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32991	Sample	BB32991	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C20	COI	45	BB32991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C20	GCACAACCGGGATCTTTACTTC	137.62115
BB32991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.9266	WGS84	BB32991	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32991_Pcan_Bot2011_IGS	PS-C20	1	BB32991_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32991	Sample	BB32991	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C20	IGS	45	BB32991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C20	CGTAATCCTCAGTACTGGCTA	137.62115
BB32991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.9266	WGS84	BB32991	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32991_Sspa_Oph2007_COI	PS-C20	1	BB32991_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32991	Sample	BB32991	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C20	COI	45	BB32991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C20	GAAGGCCTTCCGATGATC	137.62115
BB32991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.9266	WGS84	BB32991	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32991_Upin_Bot2011_COI	PS-C20	1	BB32991_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32991	Sample	BB32991	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C20	COI	45	BB32991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C20	CTTTAATTACAGCGTTTTTATTGTTGT	137.62115
BB32992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92572	WGS84	BB32992	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32992_Mgig_Bot2012_COI	PS-C21	1	BB32992_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32992	Sample	BB32992	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C21	COI	45	BB32992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C21	TCTTATTCGTTGGAGACTTTATAACCCT	137.62211
BB32993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92419	WGS84	BB32993	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32993_Cmae_Bot2011_COI	PS-C22	1	BB32993_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32993	Sample	BB32993	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C22	COI	45	BB32993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C22	ATGAACAGTCTATCCTCCTTTAG	137.62419
BB32993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92419	WGS84	BB32993	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32993_Msal_Bot2012_COI	PS-C22	1	BB32993_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32993	Sample	BB32993	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C22	COI	45	BB32993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C22	GYTAGTTCCRATGATGTTAGCTG	137.62419
BB32995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92047	WGS84	BB32995	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32995_Aamu_Bax2006_COI	PS-C24	1	BB32995_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32995	Sample	BB32995	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C24	COI	45	BB32995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C24	GCACAACCGGGATCTTTACTTC	137.62665
BB32995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92047	WGS84	BB32995	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32995_Pcan_Bot2011_IGS	PS-C24	1	BB32995_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32995	Sample	BB32995	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C24	IGS	45	BB32995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C24	CGTAATCCTCAGTACTGGCTA	137.62665
BB32995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92047	WGS84	BB32995	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32995_Sspa_Oph2007_COI	PS-C24	1	BB32995_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32995	Sample	BB32995	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C24	COI	45	BB32995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C24	GAAGGCCTTCCGATGATC	137.62665
BB32995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92047	WGS84	BB32995	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32995_Upin_Bot2011_COI	PS-C24	1	BB32995_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32995	Sample	BB32995	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C24	COI	45	BB32995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C24	CTTTAATTACAGCGTTTTTATTGTTGT	137.62665
BB32996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92203	WGS84	BB32996	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32996_Mgig_Bot2012_COI	PS-C25	1	BB32996_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32996	Sample	BB32996	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C25	COI	45	BB32996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C25	TCTTATTCGTTGGAGACTTTATAACCCT	137.62572
BB32997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92254	WGS84	BB32997	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32997_Cmae_Bot2011_COI	PS-C26	1	BB32997_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32997	Sample	BB32997	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C26	COI	45	BB32997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C26	ATGAACAGTCTATCCTCCTTTAG	137.62948
BB32997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92254	WGS84	BB32997	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32997_Msal_Bot2012_COI	PS-C26	1	BB32997_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32997	Sample	BB32997	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C26	COI	45	BB32997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C26	GYTAGTTCCRATGATGTTAGCTG	137.62948
BB32999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92169	WGS84	BB32999	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32999_Aamu_Bax2006_COI	PS-C28	1	BB32999_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32999	Sample	BB32999	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C28	COI	45	BB32999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C28	GCACAACCGGGATCTTTACTTC	137.63669
BB32999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92169	WGS84	BB32999	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32999_Pcan_Bot2011_IGS	PS-C28	1	BB32999_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32999	Sample	BB32999	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C28	IGS	45	BB32999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C28	CGTAATCCTCAGTACTGGCTA	137.63669
BB32999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92169	WGS84	BB32999	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32999_Sspa_Oph2007_COI	PS-C28	1	BB32999_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32999	Sample	BB32999	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C28	COI	45	BB32999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C28	GAAGGCCTTCCGATGATC	137.63669
BB32999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92169	WGS84	BB32999	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32999_Upin_Bot2011_COI	PS-C28	1	BB32999_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32999	Sample	BB32999	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C28	COI	45	BB32999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C28	CTTTAATTACAGCGTTTTTATTGTTGT	137.63669
BB33000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92206	WGS84	BB33000	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB33000_Cmae_Bot2011_COI	PS-C29	1	BB33000_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33000	Sample	BB33000	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C29	COI	45	BB33000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C29	ATGAACAGTCTATCCTCCTTTAG	137.63842
BB33000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92206	WGS84	BB33000	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB33000_Msal_Bot2012_COI	PS-C29	1	BB33000_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33000	Sample	BB33000	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C29	COI	45	BB33000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C29	GYTAGTTCCRATGATGTTAGCTG	137.63842
BB46880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.83416	WGS84	BB46880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.191	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46880_Aamu_Bax2006_COI	M39	1	BB46880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46880	Sample	BB46880	targeted	Present	Sample	Aamu_Bax2006_COI	M39	COI	45	BB46880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M39	GCACAACCGGGATCTTTACTTC	144.89803
BB46880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.83416	WGS84	BB46880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.298824	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46880_Sspa_Oph2007_COI	M39	1	BB46880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46880	Sample	BB46880	targeted	Present	Sample	Sspa_Oph2007_COI	M39	COI	45	BB46880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M39	GAAGGCCTTCCGATGATC	144.89803
BB46880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.83416	WGS84	BB46880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.967	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46880_Upin_Bot2011_COI	M39	1	BB46880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46880	Sample	BB46880	targeted	Present	Sample	Upin_Bot2011_COI	M39	COI	45	BB46880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M39	CTTTAATTACAGCGTTTTTATTGTTGT	144.89803
BB46884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84511	WGS84	BB46884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.056	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46884_Aamu_Bax2006_COI	M43	1	BB46884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46884	Sample	BB46884	targeted	Present	Sample	Aamu_Bax2006_COI	M43	COI	45	BB46884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M43	GCACAACCGGGATCTTTACTTC	144.93021
BB46884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84511	WGS84	BB46884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.961	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46884_Upin_Bot2011_COI	M43	1	BB46884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46884	Sample	BB46884	targeted	Present	Sample	Upin_Bot2011_COI	M43	COI	45	BB46884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M43	CTTTAATTACAGCGTTTTTATTGTTGT	144.93021
BB46886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84803	WGS84	BB46886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	26.569	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB46886_Cmae_Bot2011_COI	M45	1	BB46886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46886	Sample	BB46886	targeted	Present	Sample	Cmae_Bot2011_COI	M45	COI	45	BB46886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M45	ATGAACAGTCTATCCTCCTTTAG	144.90501
BB46888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84458	WGS84	BB46888	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.498	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46888_Aamu_Bax2006_COI	M47	1	BB46888_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46888	Sample	BB46888	targeted	Present	Sample	Aamu_Bax2006_COI	M47	COI	45	BB46888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M47	GCACAACCGGGATCTTTACTTC	144.91595
BB46888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84458	WGS84	BB46888	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.516422	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46888_Sspa_Oph2007_COI	M47	1	BB46888_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46888	Sample	BB46888	targeted	Present	Sample	Sspa_Oph2007_COI	M47	COI	45	BB46888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M47	GAAGGCCTTCCGATGATC	144.91595
BB46888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84458	WGS84	BB46888	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.65	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46888_Upin_Bot2011_COI	M47	1	BB46888_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46888	Sample	BB46888	targeted	Present	Sample	Upin_Bot2011_COI	M47	COI	45	BB46888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M47	CTTTAATTACAGCGTTTTTATTGTTGT	144.91595
BB46891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84211	WGS84	BB46891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.301	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46891_Aamu_Bax2006_COI	M50	1	BB46891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46891	Sample	BB46891	targeted	Present	Sample	Aamu_Bax2006_COI	M50	COI	45	BB46891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M50	GCACAACCGGGATCTTTACTTC	144.92977
BB46891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84211	WGS84	BB46891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.434475	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46891_Sspa_Oph2007_COI	M50	1	BB46891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46891	Sample	BB46891	targeted	Present	Sample	Sspa_Oph2007_COI	M50	COI	45	BB46891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M50	GAAGGCCTTCCGATGATC	144.92977
BB46891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84211	WGS84	BB46891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.058	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46891_Upin_Bot2011_COI	M50	1	BB46891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46891	Sample	BB46891	targeted	Present	Sample	Upin_Bot2011_COI	M50	COI	45	BB46891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M50	CTTTAATTACAGCGTTTTTATTGTTGT	144.92977
BB46895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84225	WGS84	BB46895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.534	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46895_Upin_Bot2011_COI	M54	1	BB46895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46895	Sample	BB46895	targeted	Present	Sample	Upin_Bot2011_COI	M54	COI	45	BB46895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M54	CTTTAATTACAGCGTTTTTATTGTTGT	144.92739
BB46897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84311	WGS84	BB46897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.944	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB46897_Cmae_Bot2011_COI	M56	1	BB46897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46897	Sample	BB46897	targeted	Present	Sample	Cmae_Bot2011_COI	M56	COI	45	BB46897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M56	ATGAACAGTCTATCCTCCTTTAG	144.92538
BB57686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77915	WGS84	BB57686	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57686_Aamu_Bax2006_COI	S1-04	1	BB57686_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57686	Sample	BB57686	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-04	COI	45	BB57686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-04	GCACAACCGGGATCTTTACTTC	138.51673
BB57686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77915	WGS84	BB57686	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57686_Pcan_Bot2011_IGS	S1-04	1	BB57686_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57686	Sample	BB57686	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-04	IGS	45	BB57686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-04	CGTAATCCTCAGTACTGGCTA	138.51673
BB57686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77915	WGS84	BB57686	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57686_Sspa_Oph2007_COI	S1-04	1	BB57686_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57686	Sample	BB57686	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-04	COI	45	BB57686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-04	GAAGGCCTTCCGATGATC	138.51673
BB57686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77915	WGS84	BB57686	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57686_Upin_Bot2011_COI	S1-04	1	BB57686_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57686	Sample	BB57686	targeted	Absent	Sample	Upin_Bot2011_COI	S1-04	COI	45	BB57686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.51673
BB57687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84686	WGS84	BB57687	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57687_Cjap_Sim2018_COI	S1-07	1	BB57687_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57687	Sample	BB57687	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-07	COI	45	BB57687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49747
BB57687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84686	WGS84	BB57687	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.322	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57687_Mgig_Bot2012_COI	S1-07	1	BB57687_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57687	Sample	BB57687	targeted	Present	Sample	Mgig_Bot2012_COI	S1-07	COI	45	BB57687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.49747
BB57688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8455	WGS84	BB57688	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57688_Cmae_Bot2011_COI	S1-09	1	BB57688_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57688	Sample	BB57688	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-09	COI	45	BB57688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-09	ATGAACAGTCTATCCTCCTTTAG	138.49684
BB57690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8365	WGS84	BB57690	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57690_Cjap_Sim2018_COI	S1-14	1	BB57690_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57690	Sample	BB57690	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-14	COI	45	BB57690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50875
BB57690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.8365	WGS84	BB57690	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57690_Mgig_Bot2012_COI	S1-14	1	BB57690_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57690	Sample	BB57690	targeted	Absent	Sample	Mgig_Bot2012_COI	S1-14	COI	45	BB57690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.50875
BB57691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80941	WGS84	BB57691	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57691_Cmae_Bot2011_COI	S1-17	1	BB57691_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57691	Sample	BB57691	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-17	COI	45	BB57691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-17	ATGAACAGTCTATCCTCCTTTAG	138.52052
BB57693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77643	WGS84	BB57693	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57693_Aamu_Bax2006_COI	S1-01	1	BB57693_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57693	Sample	BB57693	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-01	COI	45	BB57693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-01	GCACAACCGGGATCTTTACTTC	138.51619
BB57693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77643	WGS84	BB57693	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57693_Pcan_Bot2011_IGS	S1-01	1	BB57693_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57693	Sample	BB57693	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-01	IGS	45	BB57693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-01	CGTAATCCTCAGTACTGGCTA	138.51619
BB57693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77643	WGS84	BB57693	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57693_Sspa_Oph2007_COI	S1-01	1	BB57693_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57693	Sample	BB57693	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-01	COI	45	BB57693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-01	GAAGGCCTTCCGATGATC	138.51619
BB57694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7783	WGS84	BB57694	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57694_Cjap_Sim2018_COI	S1-03	1	BB57694_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57694	Sample	BB57694	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-03	COI	45	BB57694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51642
BB57694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.7783	WGS84	BB57694	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.274	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57694_Mgig_Bot2012_COI	S1-03	1	BB57694_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57694	Sample	BB57694	targeted	Present	Sample	Mgig_Bot2012_COI	S1-03	COI	45	BB57694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.51642
BB57695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78088	WGS84	BB57695	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57695_Cmae_Bot2011_COI	S1-05	1	BB57695_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57695	Sample	BB57695	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-05	COI	45	BB57695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-05	ATGAACAGTCTATCCTCCTTTAG	138.51745
BB57697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84714	WGS84	BB57697	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57697_Aamu_Bax2006_COI	S1-08	1	BB57697_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57697	Sample	BB57697	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-08	COI	45	BB57697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-08	GCACAACCGGGATCTTTACTTC	138.49732
BB57697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84714	WGS84	BB57697	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57697_Pcan_Bot2011_IGS	S1-08	1	BB57697_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57697	Sample	BB57697	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-08	IGS	45	BB57697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-08	CGTAATCCTCAGTACTGGCTA	138.49732
BB57697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84714	WGS84	BB57697	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57697_Sspa_Oph2007_COI	S1-08	1	BB57697_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57697	Sample	BB57697	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-08	COI	45	BB57697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-08	GAAGGCCTTCCGATGATC	138.49732
BB57697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84714	WGS84	BB57697	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57697_Upin_Bot2011_COI	S1-08	1	BB57697_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57697	Sample	BB57697	targeted	Absent	Sample	Upin_Bot2011_COI	S1-08	COI	45	BB57697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.49732
BB57698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84408	WGS84	BB57698	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57698_Cjap_Sim2018_COI	S1-10	1	BB57698_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57698	Sample	BB57698	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-10	COI	45	BB57698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49724
BB57698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84408	WGS84	BB57698	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.784	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57698_Mgig_Bot2012_COI	S1-10	1	BB57698_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57698	Sample	BB57698	targeted	Present	Sample	Mgig_Bot2012_COI	S1-10	COI	45	BB57698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.49724
BB57699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83652	WGS84	BB57699	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57699_Cmae_Bot2011_COI	S1-11	1	BB57699_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57699	Sample	BB57699	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-11	COI	45	BB57699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-11	ATGAACAGTCTATCCTCCTTTAG	138.50885
BB57701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB57701	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57701_Aamu_Bax2006_COI	S1-15	1	BB57701_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57701	Sample	BB57701	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-15	COI	45	BB57701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-15	GCACAACCGGGATCTTTACTTC	138.50926
BB57701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB57701	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57701_Pcan_Bot2011_IGS	S1-15	1	BB57701_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57701	Sample	BB57701	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-15	IGS	45	BB57701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-15	CGTAATCCTCAGTACTGGCTA	138.50926
BB57701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB57701	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57701_Sspa_Oph2007_COI	S1-15	1	BB57701_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57701	Sample	BB57701	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-15	COI	45	BB57701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-15	GAAGGCCTTCCGATGATC	138.50926
BB57701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB57701	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57701_Upin_Bot2011_COI	S1-15	1	BB57701_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57701	Sample	BB57701	targeted	Absent	Sample	Upin_Bot2011_COI	S1-15	COI	45	BB57701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-15	CTTTAATTACAGCGTTTTTATTGTTGT	138.50926
BB57702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80916	WGS84	BB57702	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57702_Cjap_Sim2018_COI	S1-16	1	BB57702_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57702	Sample	BB57702	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-16	COI	45	BB57702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51899
BB57702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80916	WGS84	BB57702	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.896	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57702_Mgig_Bot2012_COI	S1-16	1	BB57702_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57702	Sample	BB57702	targeted	Present	Sample	Mgig_Bot2012_COI	S1-16	COI	45	BB57702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.51899
BB57703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81017	WGS84	BB57703	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57703_Cmae_Bot2011_COI	S1-18	1	BB57703_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57703	Sample	BB57703	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-18	COI	45	BB57703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-18	ATGAACAGTCTATCCTCCTTTAG	138.52224
BB57705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87703	WGS84	BB57705	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57705_Aamu_Bax2006_COI	T2-13	1	BB57705_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57705	Sample	BB57705	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-13	COI	45	BB57705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-13	GCACAACCGGGATCTTTACTTC	147.34127
BB57705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87703	WGS84	BB57705	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57705_Pcan_Bot2011_IGS	T2-13	1	BB57705_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57705	Sample	BB57705	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-13	IGS	45	BB57705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-13	CGTAATCCTCAGTACTGGCTA	147.34127
BB57705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87703	WGS84	BB57705	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57705_Sspa_Oph2007_COI	T2-13	1	BB57705_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57705	Sample	BB57705	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-13	COI	45	BB57705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-13	GAAGGCCTTCCGATGATC	147.34127
BB57705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87703	WGS84	BB57705	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57705_Upin_Bot2011_COI	T2-13	1	BB57705_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57705	Sample	BB57705	targeted	Absent	Sample	Upin_Bot2011_COI	T2-13	COI	45	BB57705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.34127
BB57706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88618	WGS84	BB57706	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57706_Cjap_Sim2018_COI	T2-06	1	BB57706_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57706	Sample	BB57706	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-06	COI	45	BB57706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33863
BB57706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88618	WGS84	BB57706	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.817	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57706_Mgig_Bot2012_COI	T2-06	1	BB57706_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57706	Sample	BB57706	targeted	Present	Sample	Mgig_Bot2012_COI	T2-06	COI	45	BB57706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.33863
BB57707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88341	WGS84	BB57707	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.499	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57707_Cmae_Bot2011_COI	T2-14	1	BB57707_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57707	Sample	BB57707	targeted	Present	Sample	Cmae_Bot2011_COI	T2-14	COI	45	BB57707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-14	ATGAACAGTCTATCCTCCTTTAG	147.33708
BB57709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96028	WGS84	BB57709	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57709_Aamu_Bax2006_COI	T3-03	1	BB57709_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57709	Sample	BB57709	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-03	COI	45	BB57709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-03	GCACAACCGGGATCTTTACTTC	147.53093
BB57709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96028	WGS84	BB57709	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57709_Pcan_Bot2011_IGS	T3-03	1	BB57709_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57709	Sample	BB57709	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-03	IGS	45	BB57709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-03	CGTAATCCTCAGTACTGGCTA	147.53093
BB57709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96028	WGS84	BB57709	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57709_Sspa_Oph2007_COI	T3-03	1	BB57709_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57709	Sample	BB57709	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-03	COI	45	BB57709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-03	GAAGGCCTTCCGATGATC	147.53093
BB57709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96028	WGS84	BB57709	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57709_Upin_Bot2011_COI	T3-03	1	BB57709_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57709	Sample	BB57709	targeted	Absent	Sample	Upin_Bot2011_COI	T3-03	COI	45	BB57709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.53093
BB57710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85216	WGS84	BB57710	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57710_Cmae_Bot2011_COI	T2-08	1	BB57710_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57710	Sample	BB57710	targeted	Absent	Sample	Cmae_Bot2011_COI	T2-08	COI	45	BB57710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-08	ATGAACAGTCTATCCTCCTTTAG	147.87352
BB57712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82837	WGS84	BB57712	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57712_Aamu_Bax2006_COI	T2-01	1	BB57712_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57712	Sample	BB57712	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-01	COI	45	BB57712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-01	GCACAACCGGGATCTTTACTTC	147.30181
BB57712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82837	WGS84	BB57712	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57712_Pcan_Bot2011_IGS	T2-01	1	BB57712_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57712	Sample	BB57712	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-01	IGS	45	BB57712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-01	CGTAATCCTCAGTACTGGCTA	147.30181
BB57712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82837	WGS84	BB57712	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57712_Sspa_Oph2007_COI	T2-01	1	BB57712_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57712	Sample	BB57712	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-01	COI	45	BB57712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-01	GAAGGCCTTCCGATGATC	147.30181
BB57712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82837	WGS84	BB57712	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57712_Upin_Bot2011_COI	T2-01	1	BB57712_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57712	Sample	BB57712	targeted	Absent	Sample	Upin_Bot2011_COI	T2-01	COI	45	BB57712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.30181
BB57713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82503	WGS84	BB57713	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57713_Cjap_Sim2018_COI	T2-09	1	BB57713_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57713	Sample	BB57713	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-09	COI	45	BB57713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30534
BB57713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82503	WGS84	BB57713	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.657	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57713_Mgig_Bot2012_COI	T2-09	1	BB57713_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57713	Sample	BB57713	targeted	Present	Sample	Mgig_Bot2012_COI	T2-09	COI	45	BB57713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.30534
BB57714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87762	WGS84	BB57714	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.637	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57714_Cmae_Bot2011_COI	T2-15	1	BB57714_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57714	Sample	BB57714	targeted	Present	Sample	Cmae_Bot2011_COI	T2-15	COI	45	BB57714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-15	ATGAACAGTCTATCCTCCTTTAG	147.34147
BB57716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88465	WGS84	BB57716	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57716_Aamu_Bax2006_COI	T2-10	1	BB57716_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57716	Sample	BB57716	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-10	COI	45	BB57716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-10	GCACAACCGGGATCTTTACTTC	147.33621
BB57716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88465	WGS84	BB57716	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57716_Pcan_Bot2011_IGS	T2-10	1	BB57716_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57716	Sample	BB57716	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-10	IGS	45	BB57716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-10	CGTAATCCTCAGTACTGGCTA	147.33621
BB57716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88465	WGS84	BB57716	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57716_Sspa_Oph2007_COI	T2-10	1	BB57716_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57716	Sample	BB57716	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-10	COI	45	BB57716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-10	GAAGGCCTTCCGATGATC	147.33621
BB57716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88465	WGS84	BB57716	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57716_Upin_Bot2011_COI	T2-10	1	BB57716_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57716	Sample	BB57716	targeted	Absent	Sample	Upin_Bot2011_COI	T2-10	COI	45	BB57716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.33621
BB57717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88259	WGS84	BB57717	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57717_Cjap_Sim2018_COI	T2-16	1	BB57717_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57717	Sample	BB57717	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-16	COI	45	BB57717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33612
BB57717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88259	WGS84	BB57717	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.849	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57717_Mgig_Bot2012_COI	T2-16	1	BB57717_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57717	Sample	BB57717	targeted	Present	Sample	Mgig_Bot2012_COI	T2-16	COI	45	BB57717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.33612
BB57718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9601	WGS84	BB57718	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.159	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57718_Cmae_Bot2011_COI	T2-03	1	BB57718_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57718	Sample	BB57718	targeted	Present	Sample	Cmae_Bot2011_COI	T2-03	COI	45	BB57718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-03	ATGAACAGTCTATCCTCCTTTAG	147.53037
BB57720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84978	WGS84	BB57720	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57720_Cjap_Sim2018_COI	T3-06	1	BB57720_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57720	Sample	BB57720	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-06	COI	45	BB57720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.8733
BB57720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.84978	WGS84	BB57720	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.917	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	4/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57720_Mgig_Bot2012_COI	T3-06	1	BB57720_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57720	Sample	BB57720	targeted	Present	Sample	Mgig_Bot2012_COI	T3-06	COI	45	BB57720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.8733
BB57721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84817	WGS84	BB57721	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57721_Cmae_Bot2011_COI	T2-04	1	BB57721_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57721	Sample	BB57721	targeted	Absent	Sample	Cmae_Bot2011_COI	T2-04	COI	45	BB57721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-04	ATGAACAGTCTATCCTCCTTTAG	147.8728
BB57723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87738	WGS84	BB57723	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57723_Aamu_Bax2006_COI	T3-04	1	BB57723_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57723	Sample	BB57723	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-04	COI	45	BB57723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-04	GCACAACCGGGATCTTTACTTC	147.34147
BB57723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87738	WGS84	BB57723	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57723_Pcan_Bot2011_IGS	T3-04	1	BB57723_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57723	Sample	BB57723	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-04	IGS	45	BB57723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-04	CGTAATCCTCAGTACTGGCTA	147.34147
BB57723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87738	WGS84	BB57723	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57723_Sspa_Oph2007_COI	T3-04	1	BB57723_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57723	Sample	BB57723	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-04	COI	45	BB57723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-04	GAAGGCCTTCCGATGATC	147.34147
BB57723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87738	WGS84	BB57723	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57723_Upin_Bot2011_COI	T3-04	1	BB57723_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57723	Sample	BB57723	targeted	Absent	Sample	Upin_Bot2011_COI	T3-04	COI	45	BB57723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.34147
BB57724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84683	WGS84	BB57724	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57724_Cjap_Sim2018_COI	S2-01	1	BB57724_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57724	Sample	BB57724	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-01	COI	45	BB57724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49744
BB57724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84683	WGS84	BB57724	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57724_Mgig_Bot2012_COI	S2-01	1	BB57724_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57724	Sample	BB57724	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-01	COI	45	BB57724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.49744
BB57725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83693	WGS84	BB57725	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.854	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57725_Cmae_Bot2011_COI	S2-06	1	BB57725_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57725	Sample	BB57725	targeted	Present	Sample	Cmae_Bot2011_COI	S2-06	COI	45	BB57725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-06	ATGAACAGTCTATCCTCCTTTAG	138.50748
BB57727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83676	WGS84	BB57727	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57727_Aamu_Bax2006_COI	S2-09	1	BB57727_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57727	Sample	BB57727	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-09	COI	45	BB57727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-09	GCACAACCGGGATCTTTACTTC	138.50937
BB57727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83676	WGS84	BB57727	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57727_Pcan_Bot2011_IGS	S2-09	1	BB57727_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57727	Sample	BB57727	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-09	IGS	45	BB57727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-09	CGTAATCCTCAGTACTGGCTA	138.50937
BB57727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83676	WGS84	BB57727	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.198	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57727_Sspa_Oph2007_COI	S2-09	1	BB57727_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57727	Sample	BB57727	targeted	Present	Sample	Sspa_Oph2007_COI	S2-09	COI	45	BB57727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-09	GAAGGCCTTCCGATGATC	138.50937
BB57727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83676	WGS84	BB57727	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57727_Upin_Bot2011_COI	S2-09	1	BB57727_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57727	Sample	BB57727	targeted	Absent	Sample	Upin_Bot2011_COI	S2-09	COI	45	BB57727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.50937
BB57728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83658	WGS84	BB57728	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57728_Cjap_Sim2018_COI	S2-07	1	BB57728_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57728	Sample	BB57728	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-07	COI	45	BB57728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50823
BB57728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83658	WGS84	BB57728	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57728_Mgig_Bot2012_COI	S2-07	1	BB57728_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57728	Sample	BB57728	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-07	COI	45	BB57728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.50823
BB57729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84482	WGS84	BB57729	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57729_Cmae_Bot2011_COI	S2-04	1	BB57729_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57729	Sample	BB57729	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-04	COI	45	BB57729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-04	ATGAACAGTCTATCCTCCTTTAG	138.49676
BB57730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84664	WGS84	BB57730	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57730_Aamu_Bax2006_COI	S2-02	1	BB57730_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57730	Sample	BB57730	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-02	COI	45	BB57730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-02	GCACAACCGGGATCTTTACTTC	138.49722
BB57730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84664	WGS84	BB57730	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57730_Pcan_Bot2011_IGS	S2-02	1	BB57730_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57730	Sample	BB57730	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-02	IGS	45	BB57730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-02	CGTAATCCTCAGTACTGGCTA	138.49722
BB57730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84664	WGS84	BB57730	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.118	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57730_Sspa_Oph2007_COI	S2-02	1	BB57730_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57730	Sample	BB57730	targeted	Present	Sample	Sspa_Oph2007_COI	S2-02	COI	45	BB57730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-02	GAAGGCCTTCCGATGATC	138.49722
BB57730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84664	WGS84	BB57730	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57730_Upin_Bot2011_COI	S2-02	1	BB57730_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57730	Sample	BB57730	targeted	Absent	Sample	Upin_Bot2011_COI	S2-02	COI	45	BB57730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.49722
BB57731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82563	WGS84	BB57731	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57731_Cjap_Sim2018_COI	T3-09	1	BB57731_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57731	Sample	BB57731	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-09	COI	45	BB57731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30458
BB57731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82563	WGS84	BB57731	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.497	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57731_Mgig_Bot2012_COI	T3-09	1	BB57731_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57731	Sample	BB57731	targeted	Present	Sample	Mgig_Bot2012_COI	T3-09	COI	45	BB57731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.30458
BB57732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85213	WGS84	BB57732	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.433	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57732_Cmae_Bot2011_COI	T3-07	1	BB57732_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57732	Sample	BB57732	targeted	Present	Sample	Cmae_Bot2011_COI	T3-07	COI	45	BB57732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-07	ATGAACAGTCTATCCTCCTTTAG	147.87312
BB57734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85563	WGS84	BB57734	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57734_Aamu_Bax2006_COI	T3-12	1	BB57734_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57734	Sample	BB57734	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-12	COI	45	BB57734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-12	GCACAACCGGGATCTTTACTTC	147.87212
BB57734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85563	WGS84	BB57734	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57734_Pcan_Bot2011_IGS	T3-12	1	BB57734_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57734	Sample	BB57734	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-12	IGS	45	BB57734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-12	CGTAATCCTCAGTACTGGCTA	147.87212
BB57734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85563	WGS84	BB57734	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57734_Sspa_Oph2007_COI	T3-12	1	BB57734_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57734	Sample	BB57734	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-12	COI	45	BB57734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-12	GAAGGCCTTCCGATGATC	147.87212
BB57734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85563	WGS84	BB57734	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57734_Upin_Bot2011_COI	T3-12	1	BB57734_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57734	Sample	BB57734	targeted	Absent	Sample	Upin_Bot2011_COI	T3-12	COI	45	BB57734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.87212
BB57735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88567	WGS84	BB57735	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57735_Cjap_Sim2018_COI	T3-02	1	BB57735_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57735	Sample	BB57735	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-02	COI	45	BB57735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33476
BB57735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88567	WGS84	BB57735	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.344	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57735_Mgig_Bot2012_COI	T3-02	1	BB57735_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57735	Sample	BB57735	targeted	Present	Sample	Mgig_Bot2012_COI	T3-02	COI	45	BB57735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.33476
BB57736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82869	WGS84	BB57736	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.612	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57736_Cmae_Bot2011_COI	T3-01	1	BB57736_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57736	Sample	BB57736	targeted	Present	Sample	Cmae_Bot2011_COI	T3-01	COI	45	BB57736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-01	ATGAACAGTCTATCCTCCTTTAG	147.30215
BB57738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88476	WGS84	BB57738	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57738_Aamu_Bax2006_COI	T3-17	1	BB57738_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57738	Sample	BB57738	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-17	COI	45	BB57738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-17	GCACAACCGGGATCTTTACTTC	147.33551
BB57738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88476	WGS84	BB57738	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57738_Pcan_Bot2011_IGS	T3-17	1	BB57738_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57738	Sample	BB57738	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-17	IGS	45	BB57738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-17	CGTAATCCTCAGTACTGGCTA	147.33551
BB57738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88476	WGS84	BB57738	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57738_Sspa_Oph2007_COI	T3-17	1	BB57738_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57738	Sample	BB57738	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-17	COI	45	BB57738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-17	GAAGGCCTTCCGATGATC	147.33551
BB57738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88476	WGS84	BB57738	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.284	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57738_Upin_Bot2011_COI	T3-17	1	BB57738_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57738	Sample	BB57738	targeted	Present	Sample	Upin_Bot2011_COI	T3-17	COI	45	BB57738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-17	CTTTAATTACAGCGTTTTTATTGTTGT	147.33551
BB57741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88326	WGS84	BB57741	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57741_Aamu_Bax2006_COI	T3-16	1	BB57741_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57741	Sample	BB57741	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-16	COI	45	BB57741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-16	GCACAACCGGGATCTTTACTTC	147.33803
BB57741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88326	WGS84	BB57741	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57741_Pcan_Bot2011_IGS	T3-16	1	BB57741_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57741	Sample	BB57741	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-16	IGS	45	BB57741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-16	CGTAATCCTCAGTACTGGCTA	147.33803
BB57741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88326	WGS84	BB57741	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57741_Sspa_Oph2007_COI	T3-16	1	BB57741_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57741	Sample	BB57741	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-16	COI	45	BB57741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-16	GAAGGCCTTCCGATGATC	147.33803
BB57741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88326	WGS84	BB57741	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57741_Upin_Bot2011_COI	T3-16	1	BB57741_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57741	Sample	BB57741	targeted	Absent	Sample	Upin_Bot2011_COI	T3-16	COI	45	BB57741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.33803
BB57742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88955	WGS84	BB57742	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57742_Cjap_Sim2018_COI	T3-13	1	BB57742_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57742	Sample	BB57742	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-13	COI	45	BB57742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81318
BB57742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88955	WGS84	BB57742	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.484	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	4/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57742_Mgig_Bot2012_COI	T3-13	1	BB57742_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57742	Sample	BB57742	targeted	Present	Sample	Mgig_Bot2012_COI	T3-13	COI	45	BB57742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.81318
BB57743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88241	WGS84	BB57743	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57743_Cmae_Bot2011_COI	T3-15	1	BB57743_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57743	Sample	BB57743	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-15	COI	45	BB57743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-15	ATGAACAGTCTATCCTCCTTTAG	147.33604
BB73901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82045	WGS84	BB73901	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.789	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73901_Aamu_Bax2006_COI	MA51	1	BB73901_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73901	Sample	BB73901	targeted	Present	Sample	Aamu_Bax2006_COI	MA51	COI	45	BB73901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA51	GCACAACCGGGATCTTTACTTC	144.93041
BB73901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82045	WGS84	BB73901	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73901_Pcan_Bot2011_IGS	MA51	1	BB73901_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73901	Sample	BB73901	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA51	IGS	45	BB73901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA51	CGTAATCCTCAGTACTGGCTA	144.93041
BB73901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82045	WGS84	BB73901	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.134	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73901_Sspa_Oph2007_COI	MA51	1	BB73901_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73901	Sample	BB73901	targeted	Present	Sample	Sspa_Oph2007_COI	MA51	COI	45	BB73901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA51	GAAGGCCTTCCGATGATC	144.93041
BB73901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82045	WGS84	BB73901	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73901_Upin_Bot2011_COI	MA51	1	BB73901_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73901	Sample	BB73901	targeted	Absent	Sample	Upin_Bot2011_COI	MA51	COI	45	BB73901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA51	CTTTAATTACAGCGTTTTTATTGTTGT	144.93041
BB73902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82086	WGS84	BB73902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73902_Mgig_Bot2012_COI	MA49	1	BB73902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73902	Sample	BB73902	targeted	Absent	Sample	Mgig_Bot2012_COI	MA49	COI	45	BB73902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA49	TCTTATTCGTTGGAGACTTTATAACCCT	144.91451
BB73903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81657	WGS84	BB73903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73903_Cmae_Bot2011_COI	MA48	1	BB73903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73903	Sample	BB73903	targeted	Absent	Sample	Cmae_Bot2011_COI	MA48	COI	45	BB73903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA48	ATGAACAGTCTATCCTCCTTTAG	144.92576
BB73903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81657	WGS84	BB73903	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73903_Msal_Bot2012_COI	MA48	1	BB73903_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73903	Sample	BB73903	targeted	Absent	Sample	Msal_Bot2012_COI	MA48	COI	45	BB73903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA48	GYTAGTTCCRATGATGTTAGCTG	144.92576
BB73905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.35748	WGS84	BB73905	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73905_Aamu_Bax2006_COI	BA20	1	BB73905_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73905	Sample	BB73905	targeted	Absent	Sample	Aamu_Bax2006_COI	BA20	COI	45	BB73905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA20	GCACAACCGGGATCTTTACTTC	153.18045
BB73905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.35748	WGS84	BB73905	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73905_Pcan_Bot2011_IGS	BA20	1	BB73905_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73905	Sample	BB73905	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA20	IGS	45	BB73905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA20	CGTAATCCTCAGTACTGGCTA	153.18045
BB73905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.35748	WGS84	BB73905	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73905_Sspa_Oph2007_COI	BA20	1	BB73905_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73905	Sample	BB73905	targeted	Absent	Sample	Sspa_Oph2007_COI	BA20	COI	45	BB73905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA20	GAAGGCCTTCCGATGATC	153.18045
BB73905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.35748	WGS84	BB73905	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73905_Upin_Bot2011_COI	BA20	1	BB73905_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73905	Sample	BB73905	targeted	Absent	Sample	Upin_Bot2011_COI	BA20	COI	45	BB73905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA20	CTTTAATTACAGCGTTTTTATTGTTGT	153.18045
BB73906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82042	WGS84	BB73906	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73906_Mgig_Bot2012_COI	MA17	1	BB73906_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73906	Sample	BB73906	targeted	Absent	Sample	Mgig_Bot2012_COI	MA17	COI	45	BB73906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA17	TCTTATTCGTTGGAGACTTTATAACCCT	144.91668
BB73907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81685	WGS84	BB73907	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73907_Cmae_Bot2011_COI	MA7	1	BB73907_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73907	Sample	BB73907	targeted	Absent	Sample	Cmae_Bot2011_COI	MA7	COI	45	BB73907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA7	ATGAACAGTCTATCCTCCTTTAG	144.92339
BB73907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81685	WGS84	BB73907	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73907_Msal_Bot2012_COI	MA7	1	BB73907_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73907	Sample	BB73907	targeted	Absent	Sample	Msal_Bot2012_COI	MA7	COI	45	BB73907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA7	GYTAGTTCCRATGATGTTAGCTG	144.92339
BB73909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.4463	WGS84	BB73909	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73909_Aamu_Bax2006_COI	BA6	1	BB73909_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73909	Sample	BB73909	targeted	Absent	Sample	Aamu_Bax2006_COI	BA6	COI	45	BB73909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA6	GCACAACCGGGATCTTTACTTC	153.07695
BB73909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.4463	WGS84	BB73909	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73909_Pcan_Bot2011_IGS	BA6	1	BB73909_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73909	Sample	BB73909	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA6	IGS	45	BB73909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA6	CGTAATCCTCAGTACTGGCTA	153.07695
BB73909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.4463	WGS84	BB73909	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73909_Sspa_Oph2007_COI	BA6	1	BB73909_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73909	Sample	BB73909	targeted	Absent	Sample	Sspa_Oph2007_COI	BA6	COI	45	BB73909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA6	GAAGGCCTTCCGATGATC	153.07695
BB73909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.4463	WGS84	BB73909	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73909_Upin_Bot2011_COI	BA6	1	BB73909_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73909	Sample	BB73909	targeted	Absent	Sample	Upin_Bot2011_COI	BA6	COI	45	BB73909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA6	CTTTAATTACAGCGTTTTTATTGTTGT	153.07695
BB73910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44688	WGS84	BB73910	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73910_Cmae_Bot2011_COI	BA11	1	BB73910_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73910	Sample	BB73910	targeted	Absent	Sample	Cmae_Bot2011_COI	BA11	COI	45	BB73910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA11	ATGAACAGTCTATCCTCCTTTAG	153.07904
BB73910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44688	WGS84	BB73910	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73910_Msal_Bot2012_COI	BA11	1	BB73910_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73910	Sample	BB73910	targeted	Absent	Sample	Msal_Bot2012_COI	BA11	COI	45	BB73910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA11	GYTAGTTCCRATGATGTTAGCTG	153.07904
BB73912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81865	WGS84	BB73912	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.433	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73912_Aamu_Bax2006_COI	MA1	1	BB73912_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73912	Sample	BB73912	targeted	Present	Sample	Aamu_Bax2006_COI	MA1	COI	45	BB73912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA1	GCACAACCGGGATCTTTACTTC	144.9245
BB73912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81865	WGS84	BB73912	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73912_Pcan_Bot2011_IGS	MA1	1	BB73912_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73912	Sample	BB73912	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA1	IGS	45	BB73912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA1	CGTAATCCTCAGTACTGGCTA	144.9245
BB73912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81865	WGS84	BB73912	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.754	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73912_Sspa_Oph2007_COI	MA1	1	BB73912_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73912	Sample	BB73912	targeted	Present	Sample	Sspa_Oph2007_COI	MA1	COI	45	BB73912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA1	GAAGGCCTTCCGATGATC	144.9245
BB73912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81865	WGS84	BB73912	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73912_Upin_Bot2011_COI	MA1	1	BB73912_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73912	Sample	BB73912	targeted	Absent	Sample	Upin_Bot2011_COI	MA1	COI	45	BB73912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA1	CTTTAATTACAGCGTTTTTATTGTTGT	144.9245
BB73913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81128	WGS84	BB73913	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73913_Mgig_Bot2012_COI	GA110	1	BB73913_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73913	Sample	BB73913	targeted	Absent	Sample	Mgig_Bot2012_COI	GA110	COI	45	BB73913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA110	TCTTATTCGTTGGAGACTTTATAACCCT	144.91423
BB73914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44441	WGS84	BB73914	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73914_Cmae_Bot2011_COI	BA12	1	BB73914_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73914	Sample	BB73914	targeted	Absent	Sample	Cmae_Bot2011_COI	BA12	COI	45	BB73914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA12	ATGAACAGTCTATCCTCCTTTAG	153.07844
BB73914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44441	WGS84	BB73914	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73914_Msal_Bot2012_COI	BA12	1	BB73914_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73914	Sample	BB73914	targeted	Absent	Sample	Msal_Bot2012_COI	BA12	COI	45	BB73914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA12	GYTAGTTCCRATGATGTTAGCTG	153.07844
BB73916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82708	WGS84	BB73916	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.833	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73916_Aamu_Bax2006_COI	GA95	1	BB73916_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73916	Sample	BB73916	targeted	Present	Sample	Aamu_Bax2006_COI	GA95	COI	45	BB73916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA95	GCACAACCGGGATCTTTACTTC	144.89976
BB73916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82708	WGS84	BB73916	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73916_Pcan_Bot2011_IGS	GA95	1	BB73916_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73916	Sample	BB73916	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA95	IGS	45	BB73916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA95	CGTAATCCTCAGTACTGGCTA	144.89976
BB73916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82708	WGS84	BB73916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73916_Sspa_Oph2007_COI	GA95	1	BB73916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73916	Sample	BB73916	targeted	Absent	Sample	Sspa_Oph2007_COI	GA95	COI	45	BB73916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA95	GAAGGCCTTCCGATGATC	144.89976
BB73916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82708	WGS84	BB73916	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73916_Upin_Bot2011_COI	GA95	1	BB73916_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73916	Sample	BB73916	targeted	Absent	Sample	Upin_Bot2011_COI	GA95	COI	45	BB73916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA95	CTTTAATTACAGCGTTTTTATTGTTGT	144.89976
BB73917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81858	WGS84	BB73917	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73917_Mgig_Bot2012_COI	GA90	1	BB73917_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73917	Sample	BB73917	targeted	Absent	Sample	Mgig_Bot2012_COI	GA90	COI	45	BB73917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA90	TCTTATTCGTTGGAGACTTTATAACCCT	144.9152
BB73918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81632	WGS84	BB73918	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73918_Cmae_Bot2011_COI	GA85	1	BB73918_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73918	Sample	BB73918	targeted	Absent	Sample	Cmae_Bot2011_COI	GA85	COI	45	BB73918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA85	ATGAACAGTCTATCCTCCTTTAG	144.91413
BB73918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81632	WGS84	BB73918	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73918_Msal_Bot2012_COI	GA85	1	BB73918_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73918	Sample	BB73918	targeted	Absent	Sample	Msal_Bot2012_COI	GA85	COI	45	BB73918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA85	GYTAGTTCCRATGATGTTAGCTG	144.91413
BB73920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81899	WGS84	BB73920	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73920_Mgig_Bot2012_COI	GA75	1	BB73920_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73920	Sample	BB73920	targeted	Absent	Sample	Mgig_Bot2012_COI	GA75	COI	45	BB73920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA75	TCTTATTCGTTGGAGACTTTATAACCCT	144.91136
BB73921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82095	WGS84	BB73921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73921_Cmae_Bot2011_COI	GA70	1	BB73921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73921	Sample	BB73921	targeted	Absent	Sample	Cmae_Bot2011_COI	GA70	COI	45	BB73921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA70	ATGAACAGTCTATCCTCCTTTAG	144.90757
BB73921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82095	WGS84	BB73921	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73921_Msal_Bot2012_COI	GA70	1	BB73921_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73921	Sample	BB73921	targeted	Absent	Sample	Msal_Bot2012_COI	GA70	COI	45	BB73921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA70	GYTAGTTCCRATGATGTTAGCTG	144.90757
BB73923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44494	WGS84	BB73923	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73923_Aamu_Bax2006_COI	BA13	1	BB73923_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73923	Sample	BB73923	targeted	Absent	Sample	Aamu_Bax2006_COI	BA13	COI	45	BB73923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA13	GCACAACCGGGATCTTTACTTC	153.08106
BB73923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44494	WGS84	BB73923	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73923_Pcan_Bot2011_IGS	BA13	1	BB73923_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73923	Sample	BB73923	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA13	IGS	45	BB73923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA13	CGTAATCCTCAGTACTGGCTA	153.08106
BB73923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44494	WGS84	BB73923	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73923_Sspa_Oph2007_COI	BA13	1	BB73923_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73923	Sample	BB73923	targeted	Absent	Sample	Sspa_Oph2007_COI	BA13	COI	45	BB73923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA13	GAAGGCCTTCCGATGATC	153.08106
BB73923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44494	WGS84	BB73923	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73923_Upin_Bot2011_COI	BA13	1	BB73923_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73923	Sample	BB73923	targeted	Absent	Sample	Upin_Bot2011_COI	BA13	COI	45	BB73923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA13	CTTTAATTACAGCGTTTTTATTGTTGT	153.08106
BB73924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81631	WGS84	BB73924	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73924_Mgig_Bot2012_COI	GA50	1	BB73924_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73924	Sample	BB73924	targeted	Absent	Sample	Mgig_Bot2012_COI	GA50	COI	45	BB73924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA50	TCTTATTCGTTGGAGACTTTATAACCCT	144.91317
BB73925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81443	WGS84	BB73925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.004	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73925_Cmae_Bot2011_COI	GA45	1	BB73925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73925	Sample	BB73925	targeted	Present	Sample	Cmae_Bot2011_COI	GA45	COI	45	BB73925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA45	ATGAACAGTCTATCCTCCTTTAG	144.91251
BB73925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81443	WGS84	BB73925	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73925_Msal_Bot2012_COI	GA45	1	BB73925_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73925	Sample	BB73925	targeted	Absent	Sample	Msal_Bot2012_COI	GA45	COI	45	BB73925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA45	GYTAGTTCCRATGATGTTAGCTG	144.91251
BB73927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81859	WGS84	BB73927	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.235	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73927_Aamu_Bax2006_COI	GA35	1	BB73927_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73927	Sample	BB73927	targeted	Present	Sample	Aamu_Bax2006_COI	GA35	COI	45	BB73927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA35	GCACAACCGGGATCTTTACTTC	144.90601
BB73927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81859	WGS84	BB73927	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73927_Pcan_Bot2011_IGS	GA35	1	BB73927_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73927	Sample	BB73927	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA35	IGS	45	BB73927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA35	CGTAATCCTCAGTACTGGCTA	144.90601
BB73927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81859	WGS84	BB73927	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73927_Sspa_Oph2007_COI	GA35	1	BB73927_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73927	Sample	BB73927	targeted	Absent	Sample	Sspa_Oph2007_COI	GA35	COI	45	BB73927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA35	GAAGGCCTTCCGATGATC	144.90601
BB73927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81859	WGS84	BB73927	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73927_Upin_Bot2011_COI	GA35	1	BB73927_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73927	Sample	BB73927	targeted	Absent	Sample	Upin_Bot2011_COI	GA35	COI	45	BB73927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA35	CTTTAATTACAGCGTTTTTATTGTTGT	144.90601
BB73928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81867	WGS84	BB73928	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73928_Mgig_Bot2012_COI	GA30	1	BB73928_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73928	Sample	BB73928	targeted	Absent	Sample	Mgig_Bot2012_COI	GA30	COI	45	BB73928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA30	TCTTATTCGTTGGAGACTTTATAACCCT	144.91782
BB73929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81279	WGS84	BB73929	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73929_Cmae_Bot2011_COI	GA25	1	BB73929_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73929	Sample	BB73929	targeted	Absent	Sample	Cmae_Bot2011_COI	GA25	COI	45	BB73929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA25	ATGAACAGTCTATCCTCCTTTAG	144.91442
BB73929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81279	WGS84	BB73929	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73929_Msal_Bot2012_COI	GA25	1	BB73929_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73929	Sample	BB73929	targeted	Absent	Sample	Msal_Bot2012_COI	GA25	COI	45	BB73929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA25	GYTAGTTCCRATGATGTTAGCTG	144.91442
BB73930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37364	WGS84	BB73930	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73930_Aamu_Bax2006_COI	BA30	1	BB73930_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73930	Sample	BB73930	targeted	Absent	Sample	Aamu_Bax2006_COI	BA30	COI	45	BB73930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA30	GCACAACCGGGATCTTTACTTC	153.16922
BB73930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37364	WGS84	BB73930	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73930_Pcan_Bot2011_IGS	BA30	1	BB73930_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73930	Sample	BB73930	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA30	IGS	45	BB73930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA30	CGTAATCCTCAGTACTGGCTA	153.16922
BB73930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37364	WGS84	BB73930	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73930_Sspa_Oph2007_COI	BA30	1	BB73930_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73930	Sample	BB73930	targeted	Absent	Sample	Sspa_Oph2007_COI	BA30	COI	45	BB73930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA30	GAAGGCCTTCCGATGATC	153.16922
BB73930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37364	WGS84	BB73930	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73930_Upin_Bot2011_COI	BA30	1	BB73930_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73930	Sample	BB73930	targeted	Absent	Sample	Upin_Bot2011_COI	BA30	COI	45	BB73930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA30	CTTTAATTACAGCGTTTTTATTGTTGT	153.16922
BB73931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44806	WGS84	BB73931	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73931_Mgig_Bot2012_COI	BA17	1	BB73931_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73931	Sample	BB73931	targeted	Absent	Sample	Mgig_Bot2012_COI	BA17	COI	45	BB73931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA17	TCTTATTCGTTGGAGACTTTATAACCCT	153.094
BB73932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82371	WGS84	BB73932	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73932_Cmae_Bot2011_COI	GA10	1	BB73932_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73932	Sample	BB73932	targeted	Absent	Sample	Cmae_Bot2011_COI	GA10	COI	45	BB73932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA10	ATGAACAGTCTATCCTCCTTTAG	144.90329
BB73932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82371	WGS84	BB73932	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73932_Msal_Bot2012_COI	GA10	1	BB73932_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73932	Sample	BB73932	targeted	Absent	Sample	Msal_Bot2012_COI	GA10	COI	45	BB73932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA10	GYTAGTTCCRATGATGTTAGCTG	144.90329
BB73934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44723	WGS84	BB73934	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73934_Aamu_Bax2006_COI	BA15	1	BB73934_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73934	Sample	BB73934	targeted	Absent	Sample	Aamu_Bax2006_COI	BA15	COI	45	BB73934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA15	GCACAACCGGGATCTTTACTTC	153.09789
BB73934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44723	WGS84	BB73934	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73934_Pcan_Bot2011_IGS	BA15	1	BB73934_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73934	Sample	BB73934	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA15	IGS	45	BB73934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA15	CGTAATCCTCAGTACTGGCTA	153.09789
BB73934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44723	WGS84	BB73934	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73934_Sspa_Oph2007_COI	BA15	1	BB73934_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73934	Sample	BB73934	targeted	Absent	Sample	Sspa_Oph2007_COI	BA15	COI	45	BB73934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA15	GAAGGCCTTCCGATGATC	153.09789
BB73934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44723	WGS84	BB73934	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73934_Upin_Bot2011_COI	BA15	1	BB73934_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73934	Sample	BB73934	targeted	Absent	Sample	Upin_Bot2011_COI	BA15	COI	45	BB73934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA15	CTTTAATTACAGCGTTTTTATTGTTGT	153.09789
BB73935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85877	WGS84	BB73935	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73935_Mgig_Bot2012_COI	MA119	1	BB73935_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73935	Sample	BB73935	targeted	Absent	Sample	Mgig_Bot2012_COI	MA119	COI	45	BB73935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA119	TCTTATTCGTTGGAGACTTTATAACCCT	144.91141
BB73936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3596	WGS84	BB73936	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73936_Cmae_Bot2011_COI	BA22	1	BB73936_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73936	Sample	BB73936	targeted	Absent	Sample	Cmae_Bot2011_COI	BA22	COI	45	BB73936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA22	ATGAACAGTCTATCCTCCTTTAG	153.17773
BB73936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3596	WGS84	BB73936	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73936_Msal_Bot2012_COI	BA22	1	BB73936_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73936	Sample	BB73936	targeted	Absent	Sample	Msal_Bot2012_COI	BA22	COI	45	BB73936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA22	GYTAGTTCCRATGATGTTAGCTG	153.17773
BB73938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36254	WGS84	BB73938	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73938_Aamu_Bax2006_COI	BA24	1	BB73938_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73938	Sample	BB73938	targeted	Absent	Sample	Aamu_Bax2006_COI	BA24	COI	45	BB73938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA24	GCACAACCGGGATCTTTACTTC	153.17555
BB73938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36254	WGS84	BB73938	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73938_Pcan_Bot2011_IGS	BA24	1	BB73938_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73938	Sample	BB73938	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA24	IGS	45	BB73938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA24	CGTAATCCTCAGTACTGGCTA	153.17555
BB73938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36254	WGS84	BB73938	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73938_Sspa_Oph2007_COI	BA24	1	BB73938_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73938	Sample	BB73938	targeted	Absent	Sample	Sspa_Oph2007_COI	BA24	COI	45	BB73938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA24	GAAGGCCTTCCGATGATC	153.17555
BB73938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36254	WGS84	BB73938	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73938_Upin_Bot2011_COI	BA24	1	BB73938_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73938	Sample	BB73938	targeted	Absent	Sample	Upin_Bot2011_COI	BA24	COI	45	BB73938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA24	CTTTAATTACAGCGTTTTTATTGTTGT	153.17555
BB73939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36686	WGS84	BB73939	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73939_Mgig_Bot2012_COI	BA26	1	BB73939_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73939	Sample	BB73939	targeted	Absent	Sample	Mgig_Bot2012_COI	BA26	COI	45	BB73939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA26	TCTTATTCGTTGGAGACTTTATAACCCT	153.17334
BB73941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86508	WGS84	BB73941	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.397	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73941_Aamu_Bax2006_COI	MA81	1	BB73941_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73941	Sample	BB73941	targeted	Present	Sample	Aamu_Bax2006_COI	MA81	COI	45	BB73941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA81	GCACAACCGGGATCTTTACTTC	144.91763
BB73941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86508	WGS84	BB73941	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73941_Pcan_Bot2011_IGS	MA81	1	BB73941_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73941	Sample	BB73941	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA81	IGS	45	BB73941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA81	CGTAATCCTCAGTACTGGCTA	144.91763
BB73941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86508	WGS84	BB73941	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73941_Sspa_Oph2007_COI	MA81	1	BB73941_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73941	Sample	BB73941	targeted	Absent	Sample	Sspa_Oph2007_COI	MA81	COI	45	BB73941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA81	GAAGGCCTTCCGATGATC	144.91763
BB73941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86508	WGS84	BB73941	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.098	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73941_Upin_Bot2011_COI	MA81	1	BB73941_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73941	Sample	BB73941	targeted	Present	Sample	Upin_Bot2011_COI	MA81	COI	45	BB73941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA81	CTTTAATTACAGCGTTTTTATTGTTGT	144.91763
BB73942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86185	WGS84	BB73942	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73942_Mgig_Bot2012_COI	MA79	1	BB73942_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73942	Sample	BB73942	targeted	Absent	Sample	Mgig_Bot2012_COI	MA79	COI	45	BB73942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA79	TCTTATTCGTTGGAGACTTTATAACCCT	144.91458
BB73943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8606	WGS84	BB73943	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73943_Cmae_Bot2011_COI	MA78	1	BB73943_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73943	Sample	BB73943	targeted	Absent	Sample	Cmae_Bot2011_COI	MA78	COI	45	BB73943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA78	ATGAACAGTCTATCCTCCTTTAG	144.91438
BB73943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8606	WGS84	BB73943	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73943_Msal_Bot2012_COI	MA78	1	BB73943_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73943	Sample	BB73943	targeted	Absent	Sample	Msal_Bot2012_COI	MA78	COI	45	BB73943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA78	GYTAGTTCCRATGATGTTAGCTG	144.91438
BB73945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43165	WGS84	BB73945	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73945_Aamu_Bax2006_COI	BA65	1	BB73945_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73945	Sample	BB73945	targeted	Absent	Sample	Aamu_Bax2006_COI	BA65	COI	45	BB73945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA65	GCACAACCGGGATCTTTACTTC	153.12161
BB73945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43165	WGS84	BB73945	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73945_Pcan_Bot2011_IGS	BA65	1	BB73945_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73945	Sample	BB73945	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA65	IGS	45	BB73945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA65	CGTAATCCTCAGTACTGGCTA	153.12161
BB73945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43165	WGS84	BB73945	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73945_Sspa_Oph2007_COI	BA65	1	BB73945_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73945	Sample	BB73945	targeted	Absent	Sample	Sspa_Oph2007_COI	BA65	COI	45	BB73945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA65	GAAGGCCTTCCGATGATC	153.12161
BB73945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43165	WGS84	BB73945	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73945_Upin_Bot2011_COI	BA65	1	BB73945_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73945	Sample	BB73945	targeted	Absent	Sample	Upin_Bot2011_COI	BA65	COI	45	BB73945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA65	CTTTAATTACAGCGTTTTTATTGTTGT	153.12161
BB73946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37653	WGS84	BB73946	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73946_Mgig_Bot2012_COI	BA32	1	BB73946_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73946	Sample	BB73946	targeted	Absent	Sample	Mgig_Bot2012_COI	BA32	COI	45	BB73946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA32	TCTTATTCGTTGGAGACTTTATAACCCT	153.16689
BB73947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37905	WGS84	BB73947	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73947_Cmae_Bot2011_COI	BA34	1	BB73947_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73947	Sample	BB73947	targeted	Absent	Sample	Cmae_Bot2011_COI	BA34	COI	45	BB73947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA34	ATGAACAGTCTATCCTCCTTTAG	153.16432
BB73947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37905	WGS84	BB73947	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73947_Msal_Bot2012_COI	BA34	1	BB73947_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73947	Sample	BB73947	targeted	Absent	Sample	Msal_Bot2012_COI	BA34	COI	45	BB73947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA34	GYTAGTTCCRATGATGTTAGCTG	153.16432
BB73949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8579	WGS84	BB73949	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.028	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73949_Aamu_Bax2006_COI	MA43	1	BB73949_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73949	Sample	BB73949	targeted	Present	Sample	Aamu_Bax2006_COI	MA43	COI	45	BB73949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA43	GCACAACCGGGATCTTTACTTC	144.91104
BB73949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8579	WGS84	BB73949	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73949_Pcan_Bot2011_IGS	MA43	1	BB73949_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73949	Sample	BB73949	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA43	IGS	45	BB73949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA43	CGTAATCCTCAGTACTGGCTA	144.91104
BB73949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8579	WGS84	BB73949	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.635	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73949_Sspa_Oph2007_COI	MA43	1	BB73949_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73949	Sample	BB73949	targeted	Present	Sample	Sspa_Oph2007_COI	MA43	COI	45	BB73949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA43	GAAGGCCTTCCGATGATC	144.91104
BB73949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8579	WGS84	BB73949	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.104	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73949_Upin_Bot2011_COI	MA43	1	BB73949_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73949	Sample	BB73949	targeted	Present	Sample	Upin_Bot2011_COI	MA43	COI	45	BB73949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA43	CTTTAATTACAGCGTTTTTATTGTTGT	144.91104
BB73950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38365	WGS84	BB73950	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73950_Cmae_Bot2011_COI	BA38	1	BB73950_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73950	Sample	BB73950	targeted	Absent	Sample	Cmae_Bot2011_COI	BA38	COI	45	BB73950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA38	ATGAACAGTCTATCCTCCTTTAG	153.16045
BB73950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38365	WGS84	BB73950	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73950_Msal_Bot2012_COI	BA38	1	BB73950_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73950	Sample	BB73950	targeted	Absent	Sample	Msal_Bot2012_COI	BA38	COI	45	BB73950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA38	GYTAGTTCCRATGATGTTAGCTG	153.16045
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.272	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73952_Aamu_Bax2006_COI	MA18	1	BB73952_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Present	Sample	Aamu_Bax2006_COI	MA18	COI	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	GCACAACCGGGATCTTTACTTC	144.90781
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73952_Pcan_Bot2011_IGS	MA18	1	BB73952_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA18	IGS	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	CGTAATCCTCAGTACTGGCTA	144.90781
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.105	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73952_Sspa_Oph2007_COI	MA18	1	BB73952_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Present	Sample	Sspa_Oph2007_COI	MA18	COI	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	GAAGGCCTTCCGATGATC	144.90781
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.693	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73952_Upin_Bot2011_COI	MA18	1	BB73952_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Present	Sample	Upin_Bot2011_COI	MA18	COI	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	CTTTAATTACAGCGTTTTTATTGTTGT	144.90781
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73953_Mgig_Bot2012_COI	MA16	1	BB73953_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Absent	Sample	Mgig_Bot2012_COI	MA16	COI	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	TCTTATTCGTTGGAGACTTTATAACCCT	144.91541
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.032	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73954_Cmae_Bot2011_COI	MA14	1	BB73954_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Present	Sample	Cmae_Bot2011_COI	MA14	COI	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	ATGAACAGTCTATCCTCCTTTAG	144.91044
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73954_Hsan_Wil2021_COI	MA14	1	BB73954_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Absent	Sample	Hsan_Wil2021_COI	MA14	COI	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	CCTGGGCCGGTATAGTAGGT	144.91044
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73954_Msal_Bot2012_COI	MA14	1	BB73954_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Absent	Sample	Msal_Bot2012_COI	MA14	COI	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	GYTAGTTCCRATGATGTTAGCTG	144.91044
BB73956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38681	WGS84	BB73956	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73956_Aamu_Bax2006_COI	BA40	1	BB73956_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73956	Sample	BB73956	targeted	Absent	Sample	Aamu_Bax2006_COI	BA40	COI	45	BB73956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA40	GCACAACCGGGATCTTTACTTC	153.15913
BB73956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38681	WGS84	BB73956	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73956_Pcan_Bot2011_IGS	BA40	1	BB73956_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73956	Sample	BB73956	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA40	IGS	45	BB73956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA40	CGTAATCCTCAGTACTGGCTA	153.15913
BB73956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38681	WGS84	BB73956	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73956_Sspa_Oph2007_COI	BA40	1	BB73956_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73956	Sample	BB73956	targeted	Absent	Sample	Sspa_Oph2007_COI	BA40	COI	45	BB73956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA40	GAAGGCCTTCCGATGATC	153.15913
BB73956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38681	WGS84	BB73956	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73956_Upin_Bot2011_COI	BA40	1	BB73956_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73956	Sample	BB73956	targeted	Absent	Sample	Upin_Bot2011_COI	BA40	COI	45	BB73956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA40	CTTTAATTACAGCGTTTTTATTGTTGT	153.15913
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73957_Mgig_Bot2012_COI	MA11	1	BB73957_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Absent	Sample	Mgig_Bot2012_COI	MA11	COI	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	TCTTATTCGTTGGAGACTTTATAACCCT	144.91348
BB73958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38913	WGS84	BB73958	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73958_Cmae_Bot2011_COI	BA42	1	BB73958_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73958	Sample	BB73958	targeted	Absent	Sample	Cmae_Bot2011_COI	BA42	COI	45	BB73958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA42	ATGAACAGTCTATCCTCCTTTAG	153.15793
BB73958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38913	WGS84	BB73958	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73958_Msal_Bot2012_COI	BA42	1	BB73958_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73958	Sample	BB73958	targeted	Absent	Sample	Msal_Bot2012_COI	BA42	COI	45	BB73958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA42	GYTAGTTCCRATGATGTTAGCTG	153.15793
BB73960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.39185	WGS84	BB73960	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73960_Mgig_Bot2012_COI	BA43	1	BB73960_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73960	Sample	BB73960	targeted	Absent	Sample	Mgig_Bot2012_COI	BA43	COI	45	BB73960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA43	TCTTATTCGTTGGAGACTTTATAACCCT	153.15692
BB73961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44508	WGS84	BB73961	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73961_Cmae_Bot2011_COI	BA5	1	BB73961_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73961	Sample	BB73961	targeted	Absent	Sample	Cmae_Bot2011_COI	BA5	COI	45	BB73961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA5	ATGAACAGTCTATCCTCCTTTAG	153.07678
BB73961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44508	WGS84	BB73961	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73961_Msal_Bot2012_COI	BA5	1	BB73961_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73961	Sample	BB73961	targeted	Absent	Sample	Msal_Bot2012_COI	BA5	COI	45	BB73961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA5	GYTAGTTCCRATGATGTTAGCTG	153.07678
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.014	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73963_Aamu_Bax2006_COI	MA32	1	BB73963_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Present	Sample	Aamu_Bax2006_COI	MA32	COI	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	GCACAACCGGGATCTTTACTTC	144.90916
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73963_Pcan_Bot2011_IGS	MA32	1	BB73963_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA32	IGS	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	CGTAATCCTCAGTACTGGCTA	144.90916
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.676	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73963_Sspa_Oph2007_COI	MA32	1	BB73963_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Present	Sample	Sspa_Oph2007_COI	MA32	COI	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	GAAGGCCTTCCGATGATC	144.90916
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.823	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73963_Upin_Bot2011_COI	MA32	1	BB73963_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Present	Sample	Upin_Bot2011_COI	MA32	COI	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	CTTTAATTACAGCGTTTTTATTGTTGT	144.90916
BB73964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40078	WGS84	BB73964	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73964_Mgig_Bot2012_COI	BA44	1	BB73964_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73964	Sample	BB73964	targeted	Absent	Sample	Mgig_Bot2012_COI	BA44	COI	45	BB73964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA44	TCTTATTCGTTGGAGACTTTATAACCCT	153.16349
BB73965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40104	WGS84	BB73965	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73965_Cmae_Bot2011_COI	BA46	1	BB73965_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73965	Sample	BB73965	targeted	Absent	Sample	Cmae_Bot2011_COI	BA46	COI	45	BB73965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA46	ATGAACAGTCTATCCTCCTTTAG	153.16167
BB73965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40104	WGS84	BB73965	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73965_Msal_Bot2012_COI	BA46	1	BB73965_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73965	Sample	BB73965	targeted	Absent	Sample	Msal_Bot2012_COI	BA46	COI	45	BB73965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA46	GYTAGTTCCRATGATGTTAGCTG	153.16167
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.915	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73967_Aamu_Bax2006_COI	MA26	1	BB73967_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Present	Sample	Aamu_Bax2006_COI	MA26	COI	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	GCACAACCGGGATCTTTACTTC	144.90739
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73967_Pcan_Bot2011_IGS	MA26	1	BB73967_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA26	IGS	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	CGTAATCCTCAGTACTGGCTA	144.90739
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.98	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73967_Sspa_Oph2007_COI	MA26	1	BB73967_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Present	Sample	Sspa_Oph2007_COI	MA26	COI	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	GAAGGCCTTCCGATGATC	144.90739
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.127	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73967_Upin_Bot2011_COI	MA26	1	BB73967_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Present	Sample	Upin_Bot2011_COI	MA26	COI	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	CTTTAATTACAGCGTTTTTATTGTTGT	144.90739
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73968_Mgig_Bot2012_COI	MA24	1	BB73968_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Absent	Sample	Mgig_Bot2012_COI	MA24	COI	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	TCTTATTCGTTGGAGACTTTATAACCCT	144.90697
BB73969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40088	WGS84	BB73969	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73969_Cmae_Bot2011_COI	BA48	1	BB73969_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73969	Sample	BB73969	targeted	Absent	Sample	Cmae_Bot2011_COI	BA48	COI	45	BB73969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA48	ATGAACAGTCTATCCTCCTTTAG	153.15618
BB73969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40088	WGS84	BB73969	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73969_Msal_Bot2012_COI	BA48	1	BB73969_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73969	Sample	BB73969	targeted	Absent	Sample	Msal_Bot2012_COI	BA48	COI	45	BB73969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA48	GYTAGTTCCRATGATGTTAGCTG	153.15618
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.092	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73970_Aamu_Bax2006_COI	MA22	1	BB73970_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Present	Sample	Aamu_Bax2006_COI	MA22	COI	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	GCACAACCGGGATCTTTACTTC	144.90906
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73970_Pcan_Bot2011_IGS	MA22	1	BB73970_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA22	IGS	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	CGTAATCCTCAGTACTGGCTA	144.90906
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73970_Sspa_Oph2007_COI	MA22	1	BB73970_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Absent	Sample	Sspa_Oph2007_COI	MA22	COI	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	GAAGGCCTTCCGATGATC	144.90906
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.79	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73970_Upin_Bot2011_COI	MA22	1	BB73970_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Present	Sample	Upin_Bot2011_COI	MA22	COI	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	CTTTAATTACAGCGTTTTTATTGTTGT	144.90906
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73971_Mgig_Bot2012_COI	MA21	1	BB73971_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Absent	Sample	Mgig_Bot2012_COI	MA21	COI	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	TCTTATTCGTTGGAGACTTTATAACCCT	144.91224
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73972_Cmae_Bot2011_COI	MA126	1	BB73972_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Absent	Sample	Cmae_Bot2011_COI	MA126	COI	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	ATGAACAGTCTATCCTCCTTTAG	144.93196
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73972_Hsan_Wil2021_COI	MA126	1	BB73972_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Absent	Sample	Hsan_Wil2021_COI	MA126	COI	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	CCTGGGCCGGTATAGTAGGT	144.93196
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73972_Msal_Bot2012_COI	MA126	1	BB73972_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Absent	Sample	Msal_Bot2012_COI	MA126	COI	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	GYTAGTTCCRATGATGTTAGCTG	144.93196
BB73974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40781	WGS84	BB73974	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73974_Aamu_Bax2006_COI	BA52	1	BB73974_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73974	Sample	BB73974	targeted	Absent	Sample	Aamu_Bax2006_COI	BA52	COI	45	BB73974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA52	GCACAACCGGGATCTTTACTTC	153.1502
BB73974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40781	WGS84	BB73974	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73974_Pcan_Bot2011_IGS	BA52	1	BB73974_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73974	Sample	BB73974	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA52	IGS	45	BB73974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA52	CGTAATCCTCAGTACTGGCTA	153.1502
BB73974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40781	WGS84	BB73974	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73974_Sspa_Oph2007_COI	BA52	1	BB73974_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73974	Sample	BB73974	targeted	Absent	Sample	Sspa_Oph2007_COI	BA52	COI	45	BB73974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA52	GAAGGCCTTCCGATGATC	153.1502
BB73974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40781	WGS84	BB73974	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73974_Upin_Bot2011_COI	BA52	1	BB73974_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73974	Sample	BB73974	targeted	Absent	Sample	Upin_Bot2011_COI	BA52	COI	45	BB73974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA52	CTTTAATTACAGCGTTTTTATTGTTGT	153.1502
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73975_Mgig_Bot2012_COI	MA121	1	BB73975_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Absent	Sample	Mgig_Bot2012_COI	MA121	COI	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	TCTTATTCGTTGGAGACTTTATAACCCT	144.93272
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.932	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73976_Cmae_Bot2011_COI	MA117	1	BB73976_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Present	Sample	Cmae_Bot2011_COI	MA117	COI	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	ATGAACAGTCTATCCTCCTTTAG	144.92668
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73976_Hsan_Wil2021_COI	MA117	1	BB73976_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Absent	Sample	Hsan_Wil2021_COI	MA117	COI	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	CCTGGGCCGGTATAGTAGGT	144.92668
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73976_Msal_Bot2012_COI	MA117	1	BB73976_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Absent	Sample	Msal_Bot2012_COI	MA117	COI	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	GYTAGTTCCRATGATGTTAGCTG	144.92668
BB73978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.41523	WGS84	BB73978	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73978_Aamu_Bax2006_COI	BA53	1	BB73978_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73978	Sample	BB73978	targeted	Absent	Sample	Aamu_Bax2006_COI	BA53	COI	45	BB73978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA53	GCACAACCGGGATCTTTACTTC	153.14303
BB73978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.41523	WGS84	BB73978	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73978_Pcan_Bot2011_IGS	BA53	1	BB73978_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73978	Sample	BB73978	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA53	IGS	45	BB73978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA53	CGTAATCCTCAGTACTGGCTA	153.14303
BB73978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.41523	WGS84	BB73978	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73978_Sspa_Oph2007_COI	BA53	1	BB73978_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73978	Sample	BB73978	targeted	Absent	Sample	Sspa_Oph2007_COI	BA53	COI	45	BB73978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA53	GAAGGCCTTCCGATGATC	153.14303
BB73978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.41523	WGS84	BB73978	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73978_Upin_Bot2011_COI	BA53	1	BB73978_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73978	Sample	BB73978	targeted	Absent	Sample	Upin_Bot2011_COI	BA53	COI	45	BB73978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA53	CTTTAATTACAGCGTTTTTATTGTTGT	153.14303
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	40.547	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73979_Mgig_Bot2012_COI	MA113	1	BB73979_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Present	Sample	Mgig_Bot2012_COI	MA113	COI	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	TCTTATTCGTTGGAGACTTTATAACCCT	144.92943
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.258	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73981_Aamu_Bax2006_COI	MA108	1	BB73981_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Present	Sample	Aamu_Bax2006_COI	MA108	COI	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	GCACAACCGGGATCTTTACTTC	144.92753
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73981_Pcan_Bot2011_IGS	MA108	1	BB73981_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA108	IGS	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	CGTAATCCTCAGTACTGGCTA	144.92753
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73981_Sspa_Oph2007_COI	MA108	1	BB73981_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Absent	Sample	Sspa_Oph2007_COI	MA108	COI	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	GAAGGCCTTCCGATGATC	144.92753
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73981_Upin_Bot2011_COI	MA108	1	BB73981_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Absent	Sample	Upin_Bot2011_COI	MA108	COI	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	CTTTAATTACAGCGTTTTTATTGTTGT	144.92753
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73982_Mgig_Bot2012_COI	MA66	1	BB73982_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Absent	Sample	Mgig_Bot2012_COI	MA66	COI	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	TCTTATTCGTTGGAGACTTTATAACCCT	144.90195
BB73983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80862	WGS84	BB73983	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73983_Cmae_Bot2011_COI	GA12	1	BB73983_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73983	Sample	BB73983	targeted	Absent	Sample	Cmae_Bot2011_COI	GA12	COI	45	BB73983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA12	ATGAACAGTCTATCCTCCTTTAG	151.21623
BB73983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80862	WGS84	BB73983	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73983_Msal_Bot2012_COI	GA12	1	BB73983_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73983	Sample	BB73983	targeted	Absent	Sample	Msal_Bot2012_COI	GA12	COI	45	BB73983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA12	GYTAGTTCCRATGATGTTAGCTG	151.21623
BB73985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80663	WGS84	BB73985	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73985_Aamu_Bax2006_COI	GA9	1	BB73985_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73985	Sample	BB73985	targeted	Absent	Sample	Aamu_Bax2006_COI	GA9	COI	45	BB73985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA9	GCACAACCGGGATCTTTACTTC	151.21743
BB73985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80663	WGS84	BB73985	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73985_Pcan_Bot2011_IGS	GA9	1	BB73985_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73985	Sample	BB73985	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA9	IGS	45	BB73985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA9	CGTAATCCTCAGTACTGGCTA	151.21743
BB73985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80663	WGS84	BB73985	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73985_Sspa_Oph2007_COI	GA9	1	BB73985_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73985	Sample	BB73985	targeted	Absent	Sample	Sspa_Oph2007_COI	GA9	COI	45	BB73985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA9	GAAGGCCTTCCGATGATC	151.21743
BB73985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80663	WGS84	BB73985	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73985_Upin_Bot2011_COI	GA9	1	BB73985_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73985	Sample	BB73985	targeted	Absent	Sample	Upin_Bot2011_COI	GA9	COI	45	BB73985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA9	CTTTAATTACAGCGTTTTTATTGTTGT	151.21743
BB73986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42312	WGS84	BB73986	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73986_Mgig_Bot2012_COI	BA56	1	BB73986_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73986	Sample	BB73986	targeted	Absent	Sample	Mgig_Bot2012_COI	BA56	COI	45	BB73986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA56	TCTTATTCGTTGGAGACTTTATAACCCT	153.13199
BB73987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80807	WGS84	BB73987	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73987_Cmae_Bot2011_COI	GA7	1	BB73987_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73987	Sample	BB73987	targeted	Absent	Sample	Cmae_Bot2011_COI	GA7	COI	45	BB73987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA7	ATGAACAGTCTATCCTCCTTTAG	151.22001
BB73987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80807	WGS84	BB73987	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73987_Msal_Bot2012_COI	GA7	1	BB73987_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73987	Sample	BB73987	targeted	Absent	Sample	Msal_Bot2012_COI	GA7	COI	45	BB73987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA7	GYTAGTTCCRATGATGTTAGCTG	151.22001
BB73989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80729	WGS84	BB73989	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73989_Aamu_Bax2006_COI	GA4	1	BB73989_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73989	Sample	BB73989	targeted	Absent	Sample	Aamu_Bax2006_COI	GA4	COI	45	BB73989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA4	GCACAACCGGGATCTTTACTTC	151.21688
BB73989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80729	WGS84	BB73989	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73989_Pcan_Bot2011_IGS	GA4	1	BB73989_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73989	Sample	BB73989	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA4	IGS	45	BB73989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA4	CGTAATCCTCAGTACTGGCTA	151.21688
BB73989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80729	WGS84	BB73989	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73989_Sspa_Oph2007_COI	GA4	1	BB73989_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73989	Sample	BB73989	targeted	Absent	Sample	Sspa_Oph2007_COI	GA4	COI	45	BB73989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA4	GAAGGCCTTCCGATGATC	151.21688
BB73989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80729	WGS84	BB73989	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73989_Upin_Bot2011_COI	GA4	1	BB73989_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73989	Sample	BB73989	targeted	Absent	Sample	Upin_Bot2011_COI	GA4	COI	45	BB73989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA4	CTTTAATTACAGCGTTTTTATTGTTGT	151.21688
BB73990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42968	WGS84	BB73990	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73990_Cmae_Bot2011_COI	BA61	1	BB73990_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73990	Sample	BB73990	targeted	Absent	Sample	Cmae_Bot2011_COI	BA61	COI	45	BB73990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA61	ATGAACAGTCTATCCTCCTTTAG	153.12444
BB73990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42968	WGS84	BB73990	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73990_Msal_Bot2012_COI	BA61	1	BB73990_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73990	Sample	BB73990	targeted	Absent	Sample	Msal_Bot2012_COI	BA61	COI	45	BB73990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA61	GYTAGTTCCRATGATGTTAGCTG	153.12444
BB73992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42892	WGS84	BB73992	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73992_Aamu_Bax2006_COI	BA64	1	BB73992_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73992	Sample	BB73992	targeted	Absent	Sample	Aamu_Bax2006_COI	BA64	COI	45	BB73992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA64	GCACAACCGGGATCTTTACTTC	153.12154
BB73992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42892	WGS84	BB73992	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73992_Pcan_Bot2011_IGS	BA64	1	BB73992_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73992	Sample	BB73992	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA64	IGS	45	BB73992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA64	CGTAATCCTCAGTACTGGCTA	153.12154
BB73992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42892	WGS84	BB73992	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73992_Sspa_Oph2007_COI	BA64	1	BB73992_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73992	Sample	BB73992	targeted	Absent	Sample	Sspa_Oph2007_COI	BA64	COI	45	BB73992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA64	GAAGGCCTTCCGATGATC	153.12154
BB73992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42892	WGS84	BB73992	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73992_Upin_Bot2011_COI	BA64	1	BB73992_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73992	Sample	BB73992	targeted	Absent	Sample	Upin_Bot2011_COI	BA64	COI	45	BB73992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA64	CTTTAATTACAGCGTTTTTATTGTTGT	153.12154
BB73993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85375	WGS84	BB73993	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73993_Mgig_Bot2012_COI	MA35	1	BB73993_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73993	Sample	BB73993	targeted	Absent	Sample	Mgig_Bot2012_COI	MA35	COI	45	BB73993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA35	TCTTATTCGTTGGAGACTTTATAACCCT	151.30827
BB73994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85266	WGS84	BB73994	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73994_Cmae_Bot2011_COI	MA30	1	BB73994_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73994	Sample	BB73994	targeted	Absent	Sample	Cmae_Bot2011_COI	MA30	COI	45	BB73994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA30	ATGAACAGTCTATCCTCCTTTAG	151.31613
BB73994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85266	WGS84	BB73994	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73994_Msal_Bot2012_COI	MA30	1	BB73994_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73994	Sample	BB73994	targeted	Absent	Sample	Msal_Bot2012_COI	MA30	COI	45	BB73994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA30	GYTAGTTCCRATGATGTTAGCTG	151.31613
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.058	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73996_Aamu_Bax2006_COI	MA97	1	BB73996_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Present	Sample	Aamu_Bax2006_COI	MA97	COI	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	GCACAACCGGGATCTTTACTTC	144.93099
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73996_Pcan_Bot2011_IGS	MA97	1	BB73996_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA97	IGS	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	CGTAATCCTCAGTACTGGCTA	144.93099
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	42.842	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73996_Sspa_Oph2007_COI	MA97	1	BB73996_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Present	Sample	Sspa_Oph2007_COI	MA97	COI	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	GAAGGCCTTCCGATGATC	144.93099
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73996_Upin_Bot2011_COI	MA97	1	BB73996_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Absent	Sample	Upin_Bot2011_COI	MA97	COI	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	CTTTAATTACAGCGTTTTTATTGTTGT	144.93099
BB73997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85066	WGS84	BB73997	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73997_Mgig_Bot2012_COI	GA33	1	BB73997_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73997	Sample	BB73997	targeted	Absent	Sample	Mgig_Bot2012_COI	GA33	COI	45	BB73997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA33	TCTTATTCGTTGGAGACTTTATAACCCT	151.31174
BB73998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38217	WGS84	BB73998	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73998_Cmae_Bot2011_COI	BA37	1	BB73998_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73998	Sample	BB73998	targeted	Absent	Sample	Cmae_Bot2011_COI	BA37	COI	45	BB73998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA37	ATGAACAGTCTATCCTCCTTTAG	153.16148
BB73998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38217	WGS84	BB73998	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73998_Msal_Bot2012_COI	BA37	1	BB73998_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73998	Sample	BB73998	targeted	Absent	Sample	Msal_Bot2012_COI	BA37	COI	45	BB73998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA37	GYTAGTTCCRATGATGTTAGCTG	153.16148
BB74000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85211	WGS84	BB74000	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74000_Mgig_Bot2012_COI	GA29	1	BB74000_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74000	Sample	BB74000	targeted	Absent	Sample	Mgig_Bot2012_COI	GA29	COI	45	BB74000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA29	TCTTATTCGTTGGAGACTTTATAACCCT	151.31866
BB74001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88481	WGS84	BB74001	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74001_Cmae_Bot2011_COI	HA2	1	BB74001_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74001	Sample	BB74001	targeted	Absent	Sample	Cmae_Bot2011_COI	HA2	COI	45	BB74001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA2	ATGAACAGTCTATCCTCCTTTAG	147.3442
BB74001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88481	WGS84	BB74001	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74001_Msal_Bot2012_COI	HA2	1	BB74001_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74001	Sample	BB74001	targeted	Absent	Sample	Msal_Bot2012_COI	HA2	COI	45	BB74001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA2	GYTAGTTCCRATGATGTTAGCTG	147.3442
BB74003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8401	WGS84	BB74003	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.629	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74003_Aamu_Bax2006_COI	MA41	1	BB74003_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74003	Sample	BB74003	targeted	Present	Sample	Aamu_Bax2006_COI	MA41	COI	45	BB74003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA41	GCACAACCGGGATCTTTACTTC	144.90565
BB74003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8401	WGS84	BB74003	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74003_Pcan_Bot2011_IGS	MA41	1	BB74003_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74003	Sample	BB74003	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA41	IGS	45	BB74003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA41	CGTAATCCTCAGTACTGGCTA	144.90565
BB74003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8401	WGS84	BB74003	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	42.848	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74003_Sspa_Oph2007_COI	MA41	1	BB74003_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74003	Sample	BB74003	targeted	Present	Sample	Sspa_Oph2007_COI	MA41	COI	45	BB74003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA41	GAAGGCCTTCCGATGATC	144.90565
BB74003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8401	WGS84	BB74003	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.319	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74003_Upin_Bot2011_COI	MA41	1	BB74003_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74003	Sample	BB74003	targeted	Present	Sample	Upin_Bot2011_COI	MA41	COI	45	BB74003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA41	CTTTAATTACAGCGTTTTTATTGTTGT	144.90565
BB74004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85331	WGS84	BB74004	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74004_Mgig_Bot2012_COI	GA24	1	BB74004_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74004	Sample	BB74004	targeted	Absent	Sample	Mgig_Bot2012_COI	GA24	COI	45	BB74004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA24	TCTTATTCGTTGGAGACTTTATAACCCT	151.32146
BB74005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86492	WGS84	BB74005	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74005_Cmae_Bot2011_COI	MA77	1	BB74005_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74005	Sample	BB74005	targeted	Absent	Sample	Cmae_Bot2011_COI	MA77	COI	45	BB74005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA77	ATGAACAGTCTATCCTCCTTTAG	144.91599
BB74005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86492	WGS84	BB74005	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74005_Msal_Bot2012_COI	MA77	1	BB74005_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74005	Sample	BB74005	targeted	Absent	Sample	Msal_Bot2012_COI	MA77	COI	45	BB74005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA77	GYTAGTTCCRATGATGTTAGCTG	144.91599
BB74007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78588	WGS84	BB74007	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74007_Aamu_Bax2006_COI	MA60	1	BB74007_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74007	Sample	BB74007	targeted	Absent	Sample	Aamu_Bax2006_COI	MA60	COI	45	BB74007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA60	GCACAACCGGGATCTTTACTTC	151.17218
BB74007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78588	WGS84	BB74007	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74007_Pcan_Bot2011_IGS	MA60	1	BB74007_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74007	Sample	BB74007	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA60	IGS	45	BB74007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA60	CGTAATCCTCAGTACTGGCTA	151.17218
BB74007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78588	WGS84	BB74007	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74007_Sspa_Oph2007_COI	MA60	1	BB74007_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74007	Sample	BB74007	targeted	Absent	Sample	Sspa_Oph2007_COI	MA60	COI	45	BB74007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA60	GAAGGCCTTCCGATGATC	151.17218
BB74007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78588	WGS84	BB74007	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74007_Upin_Bot2011_COI	MA60	1	BB74007_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74007	Sample	BB74007	targeted	Absent	Sample	Upin_Bot2011_COI	MA60	COI	45	BB74007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA60	CTTTAATTACAGCGTTTTTATTGTTGT	151.17218
BB74008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78775	WGS84	BB74008	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74008_Mgig_Bot2012_COI	MA55	1	BB74008_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74008	Sample	BB74008	targeted	Absent	Sample	Mgig_Bot2012_COI	MA55	COI	45	BB74008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA55	TCTTATTCGTTGGAGACTTTATAACCCT	151.17457
BB74009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81714	WGS84	BB74009	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74009_Cmae_Bot2011_COI	MA88	1	BB74009_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74009	Sample	BB74009	targeted	Absent	Sample	Cmae_Bot2011_COI	MA88	COI	45	BB74009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA88	ATGAACAGTCTATCCTCCTTTAG	144.92188
BB74009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81714	WGS84	BB74009	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74009_Msal_Bot2012_COI	MA88	1	BB74009_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74009	Sample	BB74009	targeted	Absent	Sample	Msal_Bot2012_COI	MA88	COI	45	BB74009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA88	GYTAGTTCCRATGATGTTAGCTG	144.92188
BB74010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.794	WGS84	BB74010	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74010_Aamu_Bax2006_COI	MA45	1	BB74010_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74010	Sample	BB74010	targeted	Absent	Sample	Aamu_Bax2006_COI	MA45	COI	45	BB74010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA45	GCACAACCGGGATCTTTACTTC	151.1844
BB74010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.794	WGS84	BB74010	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74010_Pcan_Bot2011_IGS	MA45	1	BB74010_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74010	Sample	BB74010	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA45	IGS	45	BB74010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA45	CGTAATCCTCAGTACTGGCTA	151.1844
BB74010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.794	WGS84	BB74010	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74010_Sspa_Oph2007_COI	MA45	1	BB74010_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74010	Sample	BB74010	targeted	Absent	Sample	Sspa_Oph2007_COI	MA45	COI	45	BB74010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA45	GAAGGCCTTCCGATGATC	151.1844
BB74010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.794	WGS84	BB74010	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74010_Upin_Bot2011_COI	MA45	1	BB74010_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74010	Sample	BB74010	targeted	Absent	Sample	Upin_Bot2011_COI	MA45	COI	45	BB74010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA45	CTTTAATTACAGCGTTTTTATTGTTGT	151.1844
BB74011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78735	WGS84	BB74011	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74011_Mgig_Bot2012_COI	GA62	1	BB74011_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74011	Sample	BB74011	targeted	Absent	Sample	Mgig_Bot2012_COI	GA62	COI	45	BB74011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA62	TCTTATTCGTTGGAGACTTTATAACCCT	151.17788
BB74012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87588	WGS84	BB74012	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.758	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74012_Cmae_Bot2011_COI	HA6	1	BB74012_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74012	Sample	BB74012	targeted	Present	Sample	Cmae_Bot2011_COI	HA6	COI	45	BB74012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA6	ATGAACAGTCTATCCTCCTTTAG	147.34299
BB74012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87588	WGS84	BB74012	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74012_Msal_Bot2012_COI	HA6	1	BB74012_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74012	Sample	BB74012	targeted	Absent	Sample	Msal_Bot2012_COI	HA6	COI	45	BB74012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA6	GYTAGTTCCRATGATGTTAGCTG	147.34299
BB74014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78625	WGS84	BB74014	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74014_Aamu_Bax2006_COI	GA57	1	BB74014_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74014	Sample	BB74014	targeted	Absent	Sample	Aamu_Bax2006_COI	GA57	COI	45	BB74014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA57	GCACAACCGGGATCTTTACTTC	151.17631
BB74014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78625	WGS84	BB74014	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74014_Pcan_Bot2011_IGS	GA57	1	BB74014_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74014	Sample	BB74014	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA57	IGS	45	BB74014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA57	CGTAATCCTCAGTACTGGCTA	151.17631
BB74014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78625	WGS84	BB74014	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74014_Sspa_Oph2007_COI	GA57	1	BB74014_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74014	Sample	BB74014	targeted	Absent	Sample	Sspa_Oph2007_COI	GA57	COI	45	BB74014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA57	GAAGGCCTTCCGATGATC	151.17631
BB74014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78625	WGS84	BB74014	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74014_Upin_Bot2011_COI	GA57	1	BB74014_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74014	Sample	BB74014	targeted	Absent	Sample	Upin_Bot2011_COI	GA57	COI	45	BB74014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA57	CTTTAATTACAGCGTTTTTATTGTTGT	151.17631
BB74015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78668	WGS84	BB74015	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74015_Mgig_Bot2012_COI	GA56	1	BB74015_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74015	Sample	BB74015	targeted	Absent	Sample	Mgig_Bot2012_COI	GA56	COI	45	BB74015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA56	TCTTATTCGTTGGAGACTTTATAACCCT	151.17519
BB74016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88575	WGS84	BB74016	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.716	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74016_Cmae_Bot2011_COI	HA8	1	BB74016_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74016	Sample	BB74016	targeted	Present	Sample	Cmae_Bot2011_COI	HA8	COI	45	BB74016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA8	ATGAACAGTCTATCCTCCTTTAG	147.33759
BB74016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88575	WGS84	BB74016	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74016_Msal_Bot2012_COI	HA8	1	BB74016_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74016	Sample	BB74016	targeted	Absent	Sample	Msal_Bot2012_COI	HA8	COI	45	BB74016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA8	GYTAGTTCCRATGATGTTAGCTG	147.33759
BB74018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88512	WGS84	BB74018	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.317	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74018_Aamu_Bax2006_COI	HA9	1	BB74018_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74018	Sample	BB74018	targeted	Present	Sample	Aamu_Bax2006_COI	HA9	COI	45	BB74018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA9	GCACAACCGGGATCTTTACTTC	147.33365
BB74018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88512	WGS84	BB74018	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74018_Pcan_Bot2011_IGS	HA9	1	BB74018_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74018	Sample	BB74018	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA9	IGS	45	BB74018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA9	CGTAATCCTCAGTACTGGCTA	147.33365
BB74018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88512	WGS84	BB74018	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74018_Sspa_Oph2007_COI	HA9	1	BB74018_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74018	Sample	BB74018	targeted	Absent	Sample	Sspa_Oph2007_COI	HA9	COI	45	BB74018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA9	GAAGGCCTTCCGATGATC	147.33365
BB74018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88512	WGS84	BB74018	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74018_Upin_Bot2011_COI	HA9	1	BB74018_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74018	Sample	BB74018	targeted	Absent	Sample	Upin_Bot2011_COI	HA9	COI	45	BB74018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA9	CTTTAATTACAGCGTTTTTATTGTTGT	147.33365
BB74019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78956	WGS84	BB74019	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74019_Mgig_Bot2012_COI	GA51	1	BB74019_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74019	Sample	BB74019	targeted	Absent	Sample	Mgig_Bot2012_COI	GA51	COI	45	BB74019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA51	TCTTATTCGTTGGAGACTTTATAACCCT	151.17817
BB74021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7912	WGS84	BB74021	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74021_Aamu_Bax2006_COI	GA48	1	BB74021_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74021	Sample	BB74021	targeted	Absent	Sample	Aamu_Bax2006_COI	GA48	COI	45	BB74021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA48	GCACAACCGGGATCTTTACTTC	151.17933
BB74021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7912	WGS84	BB74021	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74021_Pcan_Bot2011_IGS	GA48	1	BB74021_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74021	Sample	BB74021	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA48	IGS	45	BB74021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA48	CGTAATCCTCAGTACTGGCTA	151.17933
BB74021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7912	WGS84	BB74021	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74021_Sspa_Oph2007_COI	GA48	1	BB74021_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74021	Sample	BB74021	targeted	Absent	Sample	Sspa_Oph2007_COI	GA48	COI	45	BB74021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA48	GAAGGCCTTCCGATGATC	151.17933
BB74021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7912	WGS84	BB74021	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74021_Upin_Bot2011_COI	GA48	1	BB74021_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74021	Sample	BB74021	targeted	Absent	Sample	Upin_Bot2011_COI	GA48	COI	45	BB74021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA48	CTTTAATTACAGCGTTTTTATTGTTGT	151.17933
BB74022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79159	WGS84	BB74022	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74022_Mgig_Bot2012_COI	GA47	1	BB74022_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74022	Sample	BB74022	targeted	Absent	Sample	Mgig_Bot2012_COI	GA47	COI	45	BB74022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA47	TCTTATTCGTTGGAGACTTTATAACCCT	151.1805
BB74023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88275	WGS84	BB74023	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74023_Cmae_Bot2011_COI	HA12	1	BB74023_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74023	Sample	BB74023	targeted	Absent	Sample	Cmae_Bot2011_COI	HA12	COI	45	BB74023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA12	ATGAACAGTCTATCCTCCTTTAG	147.33625
BB74023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88275	WGS84	BB74023	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74023_Msal_Bot2012_COI	HA12	1	BB74023_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74023	Sample	BB74023	targeted	Absent	Sample	Msal_Bot2012_COI	HA12	COI	45	BB74023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA12	GYTAGTTCCRATGATGTTAGCTG	147.33625
BB74025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77966	WGS84	BB74025	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74025_Aamu_Bax2006_COI	MA65	1	BB74025_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74025	Sample	BB74025	targeted	Absent	Sample	Aamu_Bax2006_COI	MA65	COI	45	BB74025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA65	GCACAACCGGGATCTTTACTTC	151.19784
BB74025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77966	WGS84	BB74025	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74025_Pcan_Bot2011_IGS	MA65	1	BB74025_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74025	Sample	BB74025	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA65	IGS	45	BB74025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA65	CGTAATCCTCAGTACTGGCTA	151.19784
BB74025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77966	WGS84	BB74025	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74025_Sspa_Oph2007_COI	MA65	1	BB74025_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74025	Sample	BB74025	targeted	Absent	Sample	Sspa_Oph2007_COI	MA65	COI	45	BB74025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA65	GAAGGCCTTCCGATGATC	151.19784
BB74025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77966	WGS84	BB74025	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74025_Upin_Bot2011_COI	MA65	1	BB74025_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74025	Sample	BB74025	targeted	Absent	Sample	Upin_Bot2011_COI	MA65	COI	45	BB74025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA65	CTTTAATTACAGCGTTTTTATTGTTGT	151.19784
BB74026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84997	WGS84	BB74026	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74026_Mgig_Bot2012_COI	HA14	1	BB74026_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74026	Sample	BB74026	targeted	Absent	Sample	Mgig_Bot2012_COI	HA14	COI	45	BB74026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA14	TCTTATTCGTTGGAGACTTTATAACCCT	147.33006
BB74027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78359	WGS84	BB74027	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74027_Cmae_Bot2011_COI	GA68	1	BB74027_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74027	Sample	BB74027	targeted	Absent	Sample	Cmae_Bot2011_COI	GA68	COI	45	BB74027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA68	ATGAACAGTCTATCCTCCTTTAG	151.20135
BB74027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78359	WGS84	BB74027	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74027_Msal_Bot2012_COI	GA68	1	BB74027_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74027	Sample	BB74027	targeted	Absent	Sample	Msal_Bot2012_COI	GA68	COI	45	BB74027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA68	GYTAGTTCCRATGATGTTAGCTG	151.20135
BB74029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84711	WGS84	BB74029	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.462	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74029_Aamu_Bax2006_COI	HA16	1	BB74029_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74029	Sample	BB74029	targeted	Present	Sample	Aamu_Bax2006_COI	HA16	COI	45	BB74029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA16	GCACAACCGGGATCTTTACTTC	147.32992
BB74029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84711	WGS84	BB74029	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74029_Pcan_Bot2011_IGS	HA16	1	BB74029_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74029	Sample	BB74029	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA16	IGS	45	BB74029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA16	CGTAATCCTCAGTACTGGCTA	147.32992
BB74029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84711	WGS84	BB74029	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74029_Sspa_Oph2007_COI	HA16	1	BB74029_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74029	Sample	BB74029	targeted	Absent	Sample	Sspa_Oph2007_COI	HA16	COI	45	BB74029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA16	GAAGGCCTTCCGATGATC	147.32992
BB74029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84711	WGS84	BB74029	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74029_Upin_Bot2011_COI	HA16	1	BB74029_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74029	Sample	BB74029	targeted	Absent	Sample	Upin_Bot2011_COI	HA16	COI	45	BB74029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA16	CTTTAATTACAGCGTTTTTATTGTTGT	147.32992
BB74030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84551	WGS84	BB74030	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.121	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74030_Cmae_Bot2011_COI	HA17	1	BB74030_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74030	Sample	BB74030	targeted	Present	Sample	Cmae_Bot2011_COI	HA17	COI	45	BB74030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA17	ATGAACAGTCTATCCTCCTTTAG	147.33086
BB74030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84551	WGS84	BB74030	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74030_Msal_Bot2012_COI	HA17	1	BB74030_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74030	Sample	BB74030	targeted	Absent	Sample	Msal_Bot2012_COI	HA17	COI	45	BB74030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA17	GYTAGTTCCRATGATGTTAGCTG	147.33086
BB74032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84416	WGS84	BB74032	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.37	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74032_Aamu_Bax2006_COI	HA18	1	BB74032_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74032	Sample	BB74032	targeted	Present	Sample	Aamu_Bax2006_COI	HA18	COI	45	BB74032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA18	GCACAACCGGGATCTTTACTTC	147.32978
BB74032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84416	WGS84	BB74032	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74032_Pcan_Bot2011_IGS	HA18	1	BB74032_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74032	Sample	BB74032	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA18	IGS	45	BB74032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA18	CGTAATCCTCAGTACTGGCTA	147.32978
BB74032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84416	WGS84	BB74032	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74032_Sspa_Oph2007_COI	HA18	1	BB74032_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74032	Sample	BB74032	targeted	Absent	Sample	Sspa_Oph2007_COI	HA18	COI	45	BB74032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA18	GAAGGCCTTCCGATGATC	147.32978
BB74032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84416	WGS84	BB74032	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74032_Upin_Bot2011_COI	HA18	1	BB74032_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74032	Sample	BB74032	targeted	Absent	Sample	Upin_Bot2011_COI	HA18	COI	45	BB74032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA18	CTTTAATTACAGCGTTTTTATTGTTGT	147.32978
BB74033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79513	WGS84	BB74033	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74033_Mgig_Bot2012_COI	GA111	1	BB74033_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74033	Sample	BB74033	targeted	Absent	Sample	Mgig_Bot2012_COI	GA111	COI	45	BB74033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA111	TCTTATTCGTTGGAGACTTTATAACCCT	151.21416
BB74034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8293	WGS84	BB74034	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.888	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74034_Cmae_Bot2011_COI	HA22	1	BB74034_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74034	Sample	BB74034	targeted	Present	Sample	Cmae_Bot2011_COI	HA22	COI	45	BB74034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA22	ATGAACAGTCTATCCTCCTTTAG	147.3183
BB74034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8293	WGS84	BB74034	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74034_Msal_Bot2012_COI	HA22	1	BB74034_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74034	Sample	BB74034	targeted	Absent	Sample	Msal_Bot2012_COI	HA22	COI	45	BB74034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA22	GYTAGTTCCRATGATGTTAGCTG	147.3183
BB74036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79117	WGS84	BB74036	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74036_Aamu_Bax2006_COI	GA71	1	BB74036_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74036	Sample	BB74036	targeted	Absent	Sample	Aamu_Bax2006_COI	GA71	COI	45	BB74036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA71	GCACAACCGGGATCTTTACTTC	151.21073
BB74036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79117	WGS84	BB74036	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74036_Pcan_Bot2011_IGS	GA71	1	BB74036_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74036	Sample	BB74036	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA71	IGS	45	BB74036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA71	CGTAATCCTCAGTACTGGCTA	151.21073
BB74036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79117	WGS84	BB74036	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74036_Sspa_Oph2007_COI	GA71	1	BB74036_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74036	Sample	BB74036	targeted	Absent	Sample	Sspa_Oph2007_COI	GA71	COI	45	BB74036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA71	GAAGGCCTTCCGATGATC	151.21073
BB74036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79117	WGS84	BB74036	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74036_Upin_Bot2011_COI	GA71	1	BB74036_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74036	Sample	BB74036	targeted	Absent	Sample	Upin_Bot2011_COI	GA71	COI	45	BB74036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA71	CTTTAATTACAGCGTTTTTATTGTTGT	151.21073
BB74037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82416	WGS84	BB74037	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74037_Mgig_Bot2012_COI	GA103	1	BB74037_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74037	Sample	BB74037	targeted	Absent	Sample	Mgig_Bot2012_COI	GA103	COI	45	BB74037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA103	TCTTATTCGTTGGAGACTTTATAACCCT	151.24835
BB74038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81951	WGS84	BB74038	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74038_Cmae_Bot2011_COI	MA105	1	BB74038_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74038	Sample	BB74038	targeted	Absent	Sample	Cmae_Bot2011_COI	MA105	COI	45	BB74038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA105	ATGAACAGTCTATCCTCCTTTAG	151.24366
BB74038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81951	WGS84	BB74038	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74038_Msal_Bot2012_COI	MA105	1	BB74038_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74038	Sample	BB74038	targeted	Absent	Sample	Msal_Bot2012_COI	MA105	COI	45	BB74038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA105	GYTAGTTCCRATGATGTTAGCTG	151.24366
BB74040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82597	WGS84	BB74040	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74040_Mgig_Bot2012_COI	MA95	1	BB74040_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74040	Sample	BB74040	targeted	Absent	Sample	Mgig_Bot2012_COI	MA95	COI	45	BB74040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA95	TCTTATTCGTTGGAGACTTTATAACCCT	151.24899
BB74041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82257	WGS84	BB74041	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74041_Cmae_Bot2011_COI	MA90	1	BB74041_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74041	Sample	BB74041	targeted	Absent	Sample	Cmae_Bot2011_COI	MA90	COI	45	BB74041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA90	ATGAACAGTCTATCCTCCTTTAG	151.24362
BB74041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82257	WGS84	BB74041	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74041_Msal_Bot2012_COI	MA90	1	BB74041_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74041	Sample	BB74041	targeted	Absent	Sample	Msal_Bot2012_COI	MA90	COI	45	BB74041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA90	GYTAGTTCCRATGATGTTAGCTG	151.24362
BB74043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82556	WGS84	BB74043	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.238	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74043_Aamu_Bax2006_COI	MA63	1	BB74043_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74043	Sample	BB74043	targeted	Present	Sample	Aamu_Bax2006_COI	MA63	COI	45	BB74043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA63	GCACAACCGGGATCTTTACTTC	144.90337
BB74043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82556	WGS84	BB74043	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74043_Pcan_Bot2011_IGS	MA63	1	BB74043_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74043	Sample	BB74043	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA63	IGS	45	BB74043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA63	CGTAATCCTCAGTACTGGCTA	144.90337
BB74043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82556	WGS84	BB74043	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74043_Sspa_Oph2007_COI	MA63	1	BB74043_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74043	Sample	BB74043	targeted	Absent	Sample	Sspa_Oph2007_COI	MA63	COI	45	BB74043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA63	GAAGGCCTTCCGATGATC	144.90337
BB74043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82556	WGS84	BB74043	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74043_Upin_Bot2011_COI	MA63	1	BB74043_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74043	Sample	BB74043	targeted	Absent	Sample	Upin_Bot2011_COI	MA63	COI	45	BB74043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA63	CTTTAATTACAGCGTTTTTATTGTTGT	144.90337
BB74044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88449	WGS84	BB74044	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74044_Mgig_Bot2012_COI	HA10	1	BB74044_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74044	Sample	BB74044	targeted	Absent	Sample	Mgig_Bot2012_COI	HA10	COI	45	BB74044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA10	TCTTATTCGTTGGAGACTTTATAACCCT	147.33547
BB74045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81955	WGS84	BB74045	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74045_Cmae_Bot2011_COI	MA91	1	BB74045_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74045	Sample	BB74045	targeted	Absent	Sample	Cmae_Bot2011_COI	MA91	COI	45	BB74045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA91	ATGAACAGTCTATCCTCCTTTAG	144.92944
BB74045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81955	WGS84	BB74045	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74045_Msal_Bot2012_COI	MA91	1	BB74045_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74045	Sample	BB74045	targeted	Absent	Sample	Msal_Bot2012_COI	MA91	COI	45	BB74045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA91	GYTAGTTCCRATGATGTTAGCTG	144.92944
BB74047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8179	WGS84	BB74047	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74047_Aamu_Bax2006_COI	GA106	1	BB74047_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74047	Sample	BB74047	targeted	Absent	Sample	Aamu_Bax2006_COI	GA106	COI	45	BB74047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA106	GCACAACCGGGATCTTTACTTC	151.24243
BB74047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8179	WGS84	BB74047	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74047_Pcan_Bot2011_IGS	GA106	1	BB74047_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74047	Sample	BB74047	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA106	IGS	45	BB74047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA106	CGTAATCCTCAGTACTGGCTA	151.24243
BB74047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8179	WGS84	BB74047	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74047_Sspa_Oph2007_COI	GA106	1	BB74047_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74047	Sample	BB74047	targeted	Absent	Sample	Sspa_Oph2007_COI	GA106	COI	45	BB74047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA106	GAAGGCCTTCCGATGATC	151.24243
BB74047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8179	WGS84	BB74047	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74047_Upin_Bot2011_COI	GA106	1	BB74047_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74047	Sample	BB74047	targeted	Absent	Sample	Upin_Bot2011_COI	GA106	COI	45	BB74047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA106	CTTTAATTACAGCGTTTTTATTGTTGT	151.24243
BB74048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82161	WGS84	BB74048	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74048_Mgig_Bot2012_COI	GA104	1	BB74048_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74048	Sample	BB74048	targeted	Absent	Sample	Mgig_Bot2012_COI	GA104	COI	45	BB74048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA104	TCTTATTCGTTGGAGACTTTATAACCCT	151.24523
BB74049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82484	WGS84	BB74049	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.511	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74049_Cmae_Bot2011_COI	MA62	1	BB74049_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74049	Sample	BB74049	targeted	Present	Sample	Cmae_Bot2011_COI	MA62	COI	45	BB74049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA62	ATGAACAGTCTATCCTCCTTTAG	144.90426
BB74049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82484	WGS84	BB74049	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74049_Msal_Bot2012_COI	MA62	1	BB74049_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74049	Sample	BB74049	targeted	Absent	Sample	Msal_Bot2012_COI	MA62	COI	45	BB74049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA62	GYTAGTTCCRATGATGTTAGCTG	144.90426
BB74050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81682	WGS84	BB74050	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74050_Aamu_Bax2006_COI	GA101	1	BB74050_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74050	Sample	BB74050	targeted	Absent	Sample	Aamu_Bax2006_COI	GA101	COI	45	BB74050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA101	GCACAACCGGGATCTTTACTTC	151.23872
BB74050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81682	WGS84	BB74050	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74050_Pcan_Bot2011_IGS	GA101	1	BB74050_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74050	Sample	BB74050	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA101	IGS	45	BB74050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA101	CGTAATCCTCAGTACTGGCTA	151.23872
BB74050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81682	WGS84	BB74050	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74050_Sspa_Oph2007_COI	GA101	1	BB74050_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74050	Sample	BB74050	targeted	Absent	Sample	Sspa_Oph2007_COI	GA101	COI	45	BB74050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA101	GAAGGCCTTCCGATGATC	151.23872
BB74050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81682	WGS84	BB74050	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74050_Upin_Bot2011_COI	GA101	1	BB74050_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74050	Sample	BB74050	targeted	Absent	Sample	Upin_Bot2011_COI	GA101	COI	45	BB74050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA101	CTTTAATTACAGCGTTTTTATTGTTGT	151.23872
BB74051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81866	WGS84	BB74051	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74051_Mgig_Bot2012_COI	GA99	1	BB74051_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74051	Sample	BB74051	targeted	Absent	Sample	Mgig_Bot2012_COI	GA99	COI	45	BB74051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA99	TCTTATTCGTTGGAGACTTTATAACCCT	151.23785
BB74052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84447	WGS84	BB74052	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74052_Cmae_Bot2011_COI	HA20	1	BB74052_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74052	Sample	BB74052	targeted	Absent	Sample	Cmae_Bot2011_COI	HA20	COI	45	BB74052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA20	ATGAACAGTCTATCCTCCTTTAG	147.32885
BB74052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84447	WGS84	BB74052	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74052_Msal_Bot2012_COI	HA20	1	BB74052_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74052	Sample	BB74052	targeted	Absent	Sample	Msal_Bot2012_COI	HA20	COI	45	BB74052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA20	GYTAGTTCCRATGATGTTAGCTG	147.32885
BB74054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82638	WGS84	BB74054	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74054_Aamu_Bax2006_COI	GA96	1	BB74054_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74054	Sample	BB74054	targeted	Absent	Sample	Aamu_Bax2006_COI	GA96	COI	45	BB74054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA96	GCACAACCGGGATCTTTACTTC	151.24668
BB74054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82638	WGS84	BB74054	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74054_Pcan_Bot2011_IGS	GA96	1	BB74054_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74054	Sample	BB74054	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA96	IGS	45	BB74054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA96	CGTAATCCTCAGTACTGGCTA	151.24668
BB74054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82638	WGS84	BB74054	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74054_Sspa_Oph2007_COI	GA96	1	BB74054_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74054	Sample	BB74054	targeted	Absent	Sample	Sspa_Oph2007_COI	GA96	COI	45	BB74054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA96	GAAGGCCTTCCGATGATC	151.24668
BB74054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82638	WGS84	BB74054	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74054_Upin_Bot2011_COI	GA96	1	BB74054_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74054	Sample	BB74054	targeted	Absent	Sample	Upin_Bot2011_COI	GA96	COI	45	BB74054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA96	CTTTAATTACAGCGTTTTTATTGTTGT	151.24668
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74055_Mgig_Bot2012_COI	MA118	1	BB74055_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Absent	Sample	Mgig_Bot2012_COI	MA118	COI	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	TCTTATTCGTTGGAGACTTTATAACCCT	144.92535
BB74056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82353	WGS84	BB74056	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74056_Cmae_Bot2011_COI	GA93	1	BB74056_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74056	Sample	BB74056	targeted	Absent	Sample	Cmae_Bot2011_COI	GA93	COI	45	BB74056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA93	ATGAACAGTCTATCCTCCTTTAG	151.24481
BB74056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82353	WGS84	BB74056	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74056_Msal_Bot2012_COI	GA93	1	BB74056_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74056	Sample	BB74056	targeted	Absent	Sample	Msal_Bot2012_COI	GA93	COI	45	BB74056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA93	GYTAGTTCCRATGATGTTAGCTG	151.24481
BB74058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82347	WGS84	BB74058	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74058_Aamu_Bax2006_COI	GA91	1	BB74058_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74058	Sample	BB74058	targeted	Absent	Sample	Aamu_Bax2006_COI	GA91	COI	45	BB74058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA91	GCACAACCGGGATCTTTACTTC	151.24428
BB74058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82347	WGS84	BB74058	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74058_Pcan_Bot2011_IGS	GA91	1	BB74058_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74058	Sample	BB74058	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA91	IGS	45	BB74058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA91	CGTAATCCTCAGTACTGGCTA	151.24428
BB74058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82347	WGS84	BB74058	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74058_Sspa_Oph2007_COI	GA91	1	BB74058_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74058	Sample	BB74058	targeted	Absent	Sample	Sspa_Oph2007_COI	GA91	COI	45	BB74058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA91	GAAGGCCTTCCGATGATC	151.24428
BB74058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82347	WGS84	BB74058	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74058_Upin_Bot2011_COI	GA91	1	BB74058_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74058	Sample	BB74058	targeted	Absent	Sample	Upin_Bot2011_COI	GA91	COI	45	BB74058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA91	CTTTAATTACAGCGTTTTTATTGTTGT	151.24428
BB74059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44339	WGS84	BB74059	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74059_Mgig_Bot2012_COI	BA1	1	BB74059_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74059	Sample	BB74059	targeted	Absent	Sample	Mgig_Bot2012_COI	BA1	COI	45	BB74059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA1	TCTTATTCGTTGGAGACTTTATAACCCT	153.07218
BB74061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82289	WGS84	BB74061	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74061_Aamu_Bax2006_COI	GA87	1	BB74061_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74061	Sample	BB74061	targeted	Absent	Sample	Aamu_Bax2006_COI	GA87	COI	45	BB74061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA87	GCACAACCGGGATCTTTACTTC	151.24174
BB74061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82289	WGS84	BB74061	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74061_Pcan_Bot2011_IGS	GA87	1	BB74061_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74061	Sample	BB74061	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA87	IGS	45	BB74061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA87	CGTAATCCTCAGTACTGGCTA	151.24174
BB74061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82289	WGS84	BB74061	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74061_Sspa_Oph2007_COI	GA87	1	BB74061_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74061	Sample	BB74061	targeted	Absent	Sample	Sspa_Oph2007_COI	GA87	COI	45	BB74061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA87	GAAGGCCTTCCGATGATC	151.24174
BB74061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82289	WGS84	BB74061	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74061_Upin_Bot2011_COI	GA87	1	BB74061_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74061	Sample	BB74061	targeted	Absent	Sample	Upin_Bot2011_COI	GA87	COI	45	BB74061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA87	CTTTAATTACAGCGTTTTTATTGTTGT	151.24174
BB74062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82186	WGS84	BB74062	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74062_Mgig_Bot2012_COI	GA86	1	BB74062_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74062	Sample	BB74062	targeted	Absent	Sample	Mgig_Bot2012_COI	GA86	COI	45	BB74062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA86	TCTTATTCGTTGGAGACTTTATAACCCT	151.24211
BB74063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8412	WGS84	BB74063	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74063_Cmae_Bot2011_COI	MA38	1	BB74063_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74063	Sample	BB74063	targeted	Absent	Sample	Cmae_Bot2011_COI	MA38	COI	45	BB74063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA38	ATGAACAGTCTATCCTCCTTTAG	144.90371
BB74063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8412	WGS84	BB74063	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74063_Msal_Bot2012_COI	MA38	1	BB74063_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74063	Sample	BB74063	targeted	Absent	Sample	Msal_Bot2012_COI	MA38	COI	45	BB74063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA38	GYTAGTTCCRATGATGTTAGCTG	144.90371
BB74065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82039	WGS84	BB74065	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74065_Aamu_Bax2006_COI	GA82	1	BB74065_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74065	Sample	BB74065	targeted	Absent	Sample	Aamu_Bax2006_COI	GA82	COI	45	BB74065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA82	GCACAACCGGGATCTTTACTTC	151.24193
BB74065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82039	WGS84	BB74065	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74065_Pcan_Bot2011_IGS	GA82	1	BB74065_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74065	Sample	BB74065	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA82	IGS	45	BB74065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA82	CGTAATCCTCAGTACTGGCTA	151.24193
BB74065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82039	WGS84	BB74065	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74065_Sspa_Oph2007_COI	GA82	1	BB74065_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74065	Sample	BB74065	targeted	Absent	Sample	Sspa_Oph2007_COI	GA82	COI	45	BB74065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA82	GAAGGCCTTCCGATGATC	151.24193
BB74065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82039	WGS84	BB74065	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74065_Upin_Bot2011_COI	GA82	1	BB74065_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74065	Sample	BB74065	targeted	Absent	Sample	Upin_Bot2011_COI	GA82	COI	45	BB74065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA82	CTTTAATTACAGCGTTTTTATTGTTGT	151.24193
BB74066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82005	WGS84	BB74066	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74066_Mgig_Bot2012_COI	GA81	1	BB74066_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74066	Sample	BB74066	targeted	Absent	Sample	Mgig_Bot2012_COI	GA81	COI	45	BB74066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA81	TCTTATTCGTTGGAGACTTTATAACCCT	151.24155
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74067_Cmae_Bot2011_COI	MA98	1	BB74067_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Absent	Sample	Cmae_Bot2011_COI	MA98	COI	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	ATGAACAGTCTATCCTCCTTTAG	144.92884
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74067_Hsan_Wil2021_COI	MA98	1	BB74067_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Absent	Sample	Hsan_Wil2021_COI	MA98	COI	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	CCTGGGCCGGTATAGTAGGT	144.92884
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74067_Msal_Bot2012_COI	MA98	1	BB74067_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Absent	Sample	Msal_Bot2012_COI	MA98	COI	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	GYTAGTTCCRATGATGTTAGCTG	144.92884
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.188	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74069_Aamu_Bax2006_COI	MA72	1	BB74069_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Present	Sample	Aamu_Bax2006_COI	MA72	COI	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	GCACAACCGGGATCTTTACTTC	144.90996
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74069_Pcan_Bot2011_IGS	MA72	1	BB74069_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA72	IGS	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	CGTAATCCTCAGTACTGGCTA	144.90996
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	42.411	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74069_Sspa_Oph2007_COI	MA72	1	BB74069_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Present	Sample	Sspa_Oph2007_COI	MA72	COI	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	GAAGGCCTTCCGATGATC	144.90996
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74069_Upin_Bot2011_COI	MA72	1	BB74069_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Absent	Sample	Upin_Bot2011_COI	MA72	COI	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	CTTTAATTACAGCGTTTTTATTGTTGT	144.90996
BB74070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82055	WGS84	BB74070	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74070_Cmae_Bot2011_COI	GA19	1	BB74070_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74070	Sample	BB74070	targeted	Absent	Sample	Cmae_Bot2011_COI	GA19	COI	45	BB74070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA19	ATGAACAGTCTATCCTCCTTTAG	151.23966
BB74070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82055	WGS84	BB74070	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74070_Msal_Bot2012_COI	GA19	1	BB74070_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74070	Sample	BB74070	targeted	Absent	Sample	Msal_Bot2012_COI	GA19	COI	45	BB74070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA19	GYTAGTTCCRATGATGTTAGCTG	151.23966
BB74072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81501	WGS84	BB74072	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74072_Aamu_Bax2006_COI	GA17	1	BB74072_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74072	Sample	BB74072	targeted	Absent	Sample	Aamu_Bax2006_COI	GA17	COI	45	BB74072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA17	GCACAACCGGGATCTTTACTTC	151.23683
BB74072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81501	WGS84	BB74072	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74072_Pcan_Bot2011_IGS	GA17	1	BB74072_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74072	Sample	BB74072	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA17	IGS	45	BB74072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA17	CGTAATCCTCAGTACTGGCTA	151.23683
BB74072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81501	WGS84	BB74072	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74072_Sspa_Oph2007_COI	GA17	1	BB74072_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74072	Sample	BB74072	targeted	Absent	Sample	Sspa_Oph2007_COI	GA17	COI	45	BB74072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA17	GAAGGCCTTCCGATGATC	151.23683
BB74072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81501	WGS84	BB74072	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74072_Upin_Bot2011_COI	GA17	1	BB74072_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74072	Sample	BB74072	targeted	Absent	Sample	Upin_Bot2011_COI	GA17	COI	45	BB74072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA17	CTTTAATTACAGCGTTTTTATTGTTGT	151.23683
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74073_Mgig_Bot2012_COI	MA54	1	BB74073_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Absent	Sample	Mgig_Bot2012_COI	MA54	COI	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	TCTTATTCGTTGGAGACTTTATAACCCT	144.92822
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74074_Cmae_Bot2011_COI	MA64	1	BB74074_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Absent	Sample	Cmae_Bot2011_COI	MA64	COI	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	ATGAACAGTCTATCCTCCTTTAG	144.92213
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74074_Hsan_Wil2021_COI	MA64	1	BB74074_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Absent	Sample	Hsan_Wil2021_COI	MA64	COI	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	CCTGGGCCGGTATAGTAGGT	144.92213
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74074_Msal_Bot2012_COI	MA64	1	BB74074_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Absent	Sample	Msal_Bot2012_COI	MA64	COI	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	GYTAGTTCCRATGATGTTAGCTG	144.92213
BB74076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83155	WGS84	BB74076	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74076_Aamu_Bax2006_COI	MA75	1	BB74076_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74076	Sample	BB74076	targeted	Absent	Sample	Aamu_Bax2006_COI	MA75	COI	45	BB74076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA75	GCACAACCGGGATCTTTACTTC	151.25742
BB74076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83155	WGS84	BB74076	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74076_Pcan_Bot2011_IGS	MA75	1	BB74076_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74076	Sample	BB74076	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA75	IGS	45	BB74076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA75	CGTAATCCTCAGTACTGGCTA	151.25742
BB74076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83155	WGS84	BB74076	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74076_Sspa_Oph2007_COI	MA75	1	BB74076_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74076	Sample	BB74076	targeted	Absent	Sample	Sspa_Oph2007_COI	MA75	COI	45	BB74076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA75	GAAGGCCTTCCGATGATC	151.25742
BB74076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83155	WGS84	BB74076	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74076_Upin_Bot2011_COI	MA75	1	BB74076_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74076	Sample	BB74076	targeted	Absent	Sample	Upin_Bot2011_COI	MA75	COI	45	BB74076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA75	CTTTAATTACAGCGTTTTTATTGTTGT	151.25742
BB74077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83422	WGS84	BB74077	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74077_Mgig_Bot2012_COI	MA40	1	BB74077_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74077	Sample	BB74077	targeted	Absent	Sample	Mgig_Bot2012_COI	MA40	COI	45	BB74077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA40	TCTTATTCGTTGGAGACTTTATAACCCT	151.27119
BB74078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83245	WGS84	BB74078	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74078_Cmae_Bot2011_COI	GA78	1	BB74078_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74078	Sample	BB74078	targeted	Absent	Sample	Cmae_Bot2011_COI	GA78	COI	45	BB74078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA78	ATGAACAGTCTATCCTCCTTTAG	151.26325
BB74078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83245	WGS84	BB74078	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74078_Msal_Bot2012_COI	GA78	1	BB74078_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74078	Sample	BB74078	targeted	Absent	Sample	Msal_Bot2012_COI	GA78	COI	45	BB74078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA78	GYTAGTTCCRATGATGTTAGCTG	151.26325
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.837	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74080_Mgig_Bot2012_COI	MA104	1	BB74080_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Present	Sample	Mgig_Bot2012_COI	MA104	COI	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	TCTTATTCGTTGGAGACTTTATAACCCT	144.90901
BB74081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83131	WGS84	BB74081	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74081_Cmae_Bot2011_COI	GA74	1	BB74081_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74081	Sample	BB74081	targeted	Absent	Sample	Cmae_Bot2011_COI	GA74	COI	45	BB74081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA74	ATGAACAGTCTATCCTCCTTTAG	151.2555
BB74081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83131	WGS84	BB74081	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74081_Msal_Bot2012_COI	GA74	1	BB74081_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74081	Sample	BB74081	targeted	Absent	Sample	Msal_Bot2012_COI	GA74	COI	45	BB74081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA74	GYTAGTTCCRATGATGTTAGCTG	151.2555
BB74083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83492	WGS84	BB74083	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74083_Aamu_Bax2006_COI	GA43	1	BB74083_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74083	Sample	BB74083	targeted	Absent	Sample	Aamu_Bax2006_COI	GA43	COI	45	BB74083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA43	GCACAACCGGGATCTTTACTTC	151.26834
BB74083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83492	WGS84	BB74083	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74083_Pcan_Bot2011_IGS	GA43	1	BB74083_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74083	Sample	BB74083	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA43	IGS	45	BB74083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA43	CGTAATCCTCAGTACTGGCTA	151.26834
BB74083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83492	WGS84	BB74083	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74083_Sspa_Oph2007_COI	GA43	1	BB74083_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74083	Sample	BB74083	targeted	Absent	Sample	Sspa_Oph2007_COI	GA43	COI	45	BB74083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA43	GAAGGCCTTCCGATGATC	151.26834
BB74083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83492	WGS84	BB74083	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74083_Upin_Bot2011_COI	GA43	1	BB74083_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74083	Sample	BB74083	targeted	Absent	Sample	Upin_Bot2011_COI	GA43	COI	45	BB74083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA43	CTTTAATTACAGCGTTTTTATTGTTGT	151.26834
BB74084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82352	WGS84	BB74084	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74084_Mgig_Bot2012_COI	MA107	1	BB74084_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74084	Sample	BB74084	targeted	Absent	Sample	Mgig_Bot2012_COI	MA107	COI	45	BB74084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA107	TCTTATTCGTTGGAGACTTTATAACCCT	144.90269
BB74085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83556	WGS84	BB74085	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74085_Cmae_Bot2011_COI	GA41	1	BB74085_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74085	Sample	BB74085	targeted	Absent	Sample	Cmae_Bot2011_COI	GA41	COI	45	BB74085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA41	ATGAACAGTCTATCCTCCTTTAG	151.27013
BB74085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83556	WGS84	BB74085	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74085_Msal_Bot2012_COI	GA41	1	BB74085_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74085	Sample	BB74085	targeted	Absent	Sample	Msal_Bot2012_COI	GA41	COI	45	BB74085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA41	GYTAGTTCCRATGATGTTAGCTG	151.27013
BB74087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8363	WGS84	BB74087	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74087_Aamu_Bax2006_COI	GA38	1	BB74087_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74087	Sample	BB74087	targeted	Absent	Sample	Aamu_Bax2006_COI	GA38	COI	45	BB74087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA38	GCACAACCGGGATCTTTACTTC	151.27496
BB74087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8363	WGS84	BB74087	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74087_Pcan_Bot2011_IGS	GA38	1	BB74087_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74087	Sample	BB74087	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA38	IGS	45	BB74087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA38	CGTAATCCTCAGTACTGGCTA	151.27496
BB74087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8363	WGS84	BB74087	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74087_Sspa_Oph2007_COI	GA38	1	BB74087_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74087	Sample	BB74087	targeted	Absent	Sample	Sspa_Oph2007_COI	GA38	COI	45	BB74087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA38	GAAGGCCTTCCGATGATC	151.27496
BB74087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8363	WGS84	BB74087	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74087_Upin_Bot2011_COI	GA38	1	BB74087_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74087	Sample	BB74087	targeted	Absent	Sample	Upin_Bot2011_COI	GA38	COI	45	BB74087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA38	CTTTAATTACAGCGTTTTTATTGTTGT	151.27496
BB74088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83619	WGS84	BB74088	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74088_Mgig_Bot2012_COI	GA37	1	BB74088_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74088	Sample	BB74088	targeted	Absent	Sample	Mgig_Bot2012_COI	GA37	COI	45	BB74088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA37	TCTTATTCGTTGGAGACTTTATAACCCT	151.2719
BB74089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44344	WGS84	BB74089	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74089_Cmae_Bot2011_COI	BA2	1	BB74089_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74089	Sample	BB74089	targeted	Absent	Sample	Cmae_Bot2011_COI	BA2	COI	45	BB74089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA2	ATGAACAGTCTATCCTCCTTTAG	153.07422
BB74089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44344	WGS84	BB74089	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74089_Msal_Bot2012_COI	BA2	1	BB74089_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74089	Sample	BB74089	targeted	Absent	Sample	Msal_Bot2012_COI	BA2	COI	45	BB74089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA2	GYTAGTTCCRATGATGTTAGCTG	153.07422
BB74187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85244	WGS84	BB74187	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74187_Cjap_Sim2018_COI	T1-01	1	BB74187_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74187	Sample	BB74187	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-01	COI	45	BB74187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.86938
BB74187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85244	WGS84	BB74187	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.932	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74187_Mgig_Bot2012_COI	T1-01	1	BB74187_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74187	Sample	BB74187	targeted	Present	Sample	Mgig_Bot2012_COI	T1-01	COI	45	BB74187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.86938
BB74188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85433	WGS84	BB74188	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74188_Cmae_Bot2011_COI	T1-09	1	BB74188_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74188	Sample	BB74188	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-09	COI	45	BB74188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-09	ATGAACAGTCTATCCTCCTTTAG	147.87329
BB74190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95973	WGS84	BB74190	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74190_Cjap_Sim2018_COI	T1-02	1	BB74190_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74190	Sample	BB74190	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-02	COI	45	BB74190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52492
BB74190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.95973	WGS84	BB74190	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.26	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74190_Mgig_Bot2012_COI	T1-02	1	BB74190_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74190	Sample	BB74190	targeted	Present	Sample	Mgig_Bot2012_COI	T1-02	COI	45	BB74190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.52492
BB74191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96918	WGS84	BB74191	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.073	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74191_Cmae_Bot2011_COI	T1-10	1	BB74191_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74191	Sample	BB74191	targeted	Present	Sample	Cmae_Bot2011_COI	T1-10	COI	45	BB74191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-10	ATGAACAGTCTATCCTCCTTTAG	147.51603
BB74193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88525	WGS84	BB74193	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74193_Aamu_Bax2006_COI	T1-03	1	BB74193_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74193	Sample	BB74193	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-03	COI	45	BB74193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-03	GCACAACCGGGATCTTTACTTC	147.3358
BB74193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88525	WGS84	BB74193	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74193_Pcan_Bot2011_IGS	T1-03	1	BB74193_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74193	Sample	BB74193	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-03	IGS	45	BB74193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-03	CGTAATCCTCAGTACTGGCTA	147.3358
BB74193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88525	WGS84	BB74193	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74193_Sspa_Oph2007_COI	T1-03	1	BB74193_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74193	Sample	BB74193	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-03	COI	45	BB74193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-03	GAAGGCCTTCCGATGATC	147.3358
BB74193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88525	WGS84	BB74193	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74193_Upin_Bot2011_COI	T1-03	1	BB74193_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74193	Sample	BB74193	targeted	Absent	Sample	Upin_Bot2011_COI	T1-03	COI	45	BB74193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.3358
BB74194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88441	WGS84	BB74194	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74194_Cjap_Sim2018_COI	T1-11	1	BB74194_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74194	Sample	BB74194	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-11	COI	45	BB74194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33665
BB74194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88441	WGS84	BB74194	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.237	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74194_Mgig_Bot2012_COI	T1-11	1	BB74194_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74194	Sample	BB74194	targeted	Present	Sample	Mgig_Bot2012_COI	T1-11	COI	45	BB74194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33665
BB74195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88393	WGS84	BB74195	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74195_Cmae_Bot2011_COI	T1-19	1	BB74195_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74195	Sample	BB74195	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-19	COI	45	BB74195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-19	ATGAACAGTCTATCCTCCTTTAG	147.33802
BB74197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82601	WGS84	BB74197	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74197_Aamu_Bax2006_COI	T1-12	1	BB74197_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74197	Sample	BB74197	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-12	COI	45	BB74197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-12	GCACAACCGGGATCTTTACTTC	147.30451
BB74197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82601	WGS84	BB74197	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74197_Pcan_Bot2011_IGS	T1-12	1	BB74197_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74197	Sample	BB74197	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-12	IGS	45	BB74197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-12	CGTAATCCTCAGTACTGGCTA	147.30451
BB74197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82601	WGS84	BB74197	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74197_Sspa_Oph2007_COI	T1-12	1	BB74197_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74197	Sample	BB74197	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-12	COI	45	BB74197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-12	GAAGGCCTTCCGATGATC	147.30451
BB74197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82601	WGS84	BB74197	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74197_Upin_Bot2011_COI	T1-12	1	BB74197_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74197	Sample	BB74197	targeted	Absent	Sample	Upin_Bot2011_COI	T1-12	COI	45	BB74197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.30451
BB74198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82426	WGS84	BB74198	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74198_Cjap_Sim2018_COI	T1-20	1	BB74198_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74198	Sample	BB74198	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-20	COI	45	BB74198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-20	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30787
BB74198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82426	WGS84	BB74198	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.899	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74198_Mgig_Bot2012_COI	T1-20	1	BB74198_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74198	Sample	BB74198	targeted	Present	Sample	Mgig_Bot2012_COI	T1-20	COI	45	BB74198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-20	TCTTATTCGTTGGAGACTTTATAACCCT	147.30787
BB74199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84579	WGS84	BB74199	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.309	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74199_Cmae_Bot2011_COI	S2-03	1	BB74199_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74199	Sample	BB74199	targeted	Present	Sample	Cmae_Bot2011_COI	S2-03	COI	45	BB74199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-03	ATGAACAGTCTATCCTCCTTTAG	138.49682
BB74201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83684	WGS84	BB74201	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74201_Aamu_Bax2006_COI	S2-08	1	BB74201_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74201	Sample	BB74201	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-08	COI	45	BB74201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-08	GCACAACCGGGATCTTTACTTC	138.50934
BB74201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83684	WGS84	BB74201	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74201_Pcan_Bot2011_IGS	S2-08	1	BB74201_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74201	Sample	BB74201	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-08	IGS	45	BB74201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-08	CGTAATCCTCAGTACTGGCTA	138.50934
BB74201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83684	WGS84	BB74201	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.553	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74201_Sspa_Oph2007_COI	S2-08	1	BB74201_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74201	Sample	BB74201	targeted	Present	Sample	Sspa_Oph2007_COI	S2-08	COI	45	BB74201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-08	GAAGGCCTTCCGATGATC	138.50934
BB74201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83684	WGS84	BB74201	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74201_Upin_Bot2011_COI	S2-08	1	BB74201_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74201	Sample	BB74201	targeted	Absent	Sample	Upin_Bot2011_COI	S2-08	COI	45	BB74201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.50934
BB74202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78745	WGS84	BB74202	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74202_Cjap_Sim2018_COI	S2-11	1	BB74202_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74202	Sample	BB74202	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-11	COI	45	BB74202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51416
BB74202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78745	WGS84	BB74202	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74202_Mgig_Bot2012_COI	S2-11	1	BB74202_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74202	Sample	BB74202	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-11	COI	45	BB74202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51416
BB74203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83626	WGS84	BB74203	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.544	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74203_Cmae_Bot2011_COI	S2-10	1	BB74203_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74203	Sample	BB74203	targeted	Present	Sample	Cmae_Bot2011_COI	S2-10	COI	45	BB74203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-10	ATGAACAGTCTATCCTCCTTTAG	138.50837
BB74205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80954	WGS84	BB74205	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74205_Aamu_Bax2006_COI	S2-19	1	BB74205_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74205	Sample	BB74205	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-19	COI	45	BB74205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-19	GCACAACCGGGATCTTTACTTC	138.51877
BB74205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80954	WGS84	BB74205	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74205_Pcan_Bot2011_IGS	S2-19	1	BB74205_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74205	Sample	BB74205	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-19	IGS	45	BB74205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-19	CGTAATCCTCAGTACTGGCTA	138.51877
BB74205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80954	WGS84	BB74205	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.377	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74205_Sspa_Oph2007_COI	S2-19	1	BB74205_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74205	Sample	BB74205	targeted	Present	Sample	Sspa_Oph2007_COI	S2-19	COI	45	BB74205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-19	GAAGGCCTTCCGATGATC	138.51877
BB74205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80954	WGS84	BB74205	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74205_Upin_Bot2011_COI	S2-19	1	BB74205_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74205	Sample	BB74205	targeted	Absent	Sample	Upin_Bot2011_COI	S2-19	COI	45	BB74205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.51877
BB74206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78616	WGS84	BB74206	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74206_Cjap_Sim2018_COI	S2-14	1	BB74206_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74206	Sample	BB74206	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-14	COI	45	BB74206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51483
BB74206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78616	WGS84	BB74206	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74206_Mgig_Bot2012_COI	S2-14	1	BB74206_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74206	Sample	BB74206	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-14	COI	45	BB74206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.51483
BB75217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80872	WGS84	BB75217	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75217_Aamu_Bax2006_COI	S2-16	1	BB75217_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75217	Sample	BB75217	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-16	COI	45	BB75217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-16	GCACAACCGGGATCTTTACTTC	138.51653
BB75217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80872	WGS84	BB75217	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75217_Pcan_Bot2011_IGS	S2-16	1	BB75217_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75217	Sample	BB75217	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-16	IGS	45	BB75217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-16	CGTAATCCTCAGTACTGGCTA	138.51653
BB75217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80872	WGS84	BB75217	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.046	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75217_Sspa_Oph2007_COI	S2-16	1	BB75217_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75217	Sample	BB75217	targeted	Present	Sample	Sspa_Oph2007_COI	S2-16	COI	45	BB75217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-16	GAAGGCCTTCCGATGATC	138.51653
BB75217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80872	WGS84	BB75217	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75217_Upin_Bot2011_COI	S2-16	1	BB75217_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75217	Sample	BB75217	targeted	Absent	Sample	Upin_Bot2011_COI	S2-16	COI	45	BB75217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.51653
BB75218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80908	WGS84	BB75218	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75218_Cjap_Sim2018_COI	S2-17	1	BB75218_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75218	Sample	BB75218	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-17	COI	45	BB75218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51763
BB75218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80908	WGS84	BB75218	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75218_Mgig_Bot2012_COI	S2-17	1	BB75218_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75218	Sample	BB75218	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-17	COI	45	BB75218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.51763
BB75219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80944	WGS84	BB75219	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75219_Cmae_Bot2011_COI	S2-18	1	BB75219_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75219	Sample	BB75219	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-18	COI	45	BB75219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-18	ATGAACAGTCTATCCTCCTTTAG	138.51875
BB75221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85207	WGS84	BB75221	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75221_Cjap_Sim2018_COI	T1-05	1	BB75221_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75221	Sample	BB75221	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-05	COI	45	BB75221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87165
BB75221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85207	WGS84	BB75221	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75221_Mgig_Bot2012_COI	T1-05	1	BB75221_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75221	Sample	BB75221	targeted	Absent	Sample	Mgig_Bot2012_COI	T1-05	COI	45	BB75221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.87165
BB75222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85712	WGS84	BB75222	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75222_Cmae_Bot2011_COI	T1-13	1	BB75222_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75222	Sample	BB75222	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-13	COI	45	BB75222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-13	ATGAACAGTCTATCCTCCTTTAG	147.87617
BB75225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88573	WGS84	BB75225	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75225_Cjap_Sim2018_COI	T1-07	1	BB75225_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75225	Sample	BB75225	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-07	COI	45	BB75225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33828
BB75225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88573	WGS84	BB75225	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75225_Mgig_Bot2012_COI	T1-07	1	BB75225_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75225	Sample	BB75225	targeted	Absent	Sample	Mgig_Bot2012_COI	T1-07	COI	45	BB75225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.33828
BB75226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87458	WGS84	BB75226	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.513	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75226_Cmae_Bot2011_COI	T1-15	1	BB75226_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75226	Sample	BB75226	targeted	Present	Sample	Cmae_Bot2011_COI	T1-15	COI	45	BB75226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-15	ATGAACAGTCTATCCTCCTTTAG	147.33635
BB75228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82735	WGS84	BB75228	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75228_Aamu_Bax2006_COI	T1-16	1	BB75228_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75228	Sample	BB75228	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-16	COI	45	BB75228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-16	GCACAACCGGGATCTTTACTTC	147.3021
BB75228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82735	WGS84	BB75228	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75228_Pcan_Bot2011_IGS	T1-16	1	BB75228_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75228	Sample	BB75228	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-16	IGS	45	BB75228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-16	CGTAATCCTCAGTACTGGCTA	147.3021
BB75228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82735	WGS84	BB75228	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75228_Sspa_Oph2007_COI	T1-16	1	BB75228_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75228	Sample	BB75228	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-16	COI	45	BB75228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-16	GAAGGCCTTCCGATGATC	147.3021
BB75228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82735	WGS84	BB75228	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75228_Upin_Bot2011_COI	T1-16	1	BB75228_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75228	Sample	BB75228	targeted	Absent	Sample	Upin_Bot2011_COI	T1-16	COI	45	BB75228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.3021
BB75229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82712	WGS84	BB75229	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75229_Cjap_Sim2018_COI	T4-02	1	BB75229_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75229	Sample	BB75229	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-02	COI	45	BB75229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3019
BB75229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82712	WGS84	BB75229	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75229_Mgig_Bot2012_COI	T4-02	1	BB75229_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75229	Sample	BB75229	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-02	COI	45	BB75229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.3019
BB75231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89813	WGS84	BB75231	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.16	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75231_Aamu_Bax2006_COI	T4-06	1	BB75231_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75231	Sample	BB75231	targeted	Present	Sample	Aamu_Bax2006_COI	T4-06	COI	45	BB75231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-06	GCACAACCGGGATCTTTACTTC	147.34208
BB75231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89813	WGS84	BB75231	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75231_Pcan_Bot2011_IGS	T4-06	1	BB75231_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75231	Sample	BB75231	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-06	IGS	45	BB75231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-06	CGTAATCCTCAGTACTGGCTA	147.34208
BB75231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89813	WGS84	BB75231	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75231_Sspa_Oph2007_COI	T4-06	1	BB75231_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75231	Sample	BB75231	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-06	COI	45	BB75231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-06	GAAGGCCTTCCGATGATC	147.34208
BB75231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89813	WGS84	BB75231	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75231_Upin_Bot2011_COI	T4-06	1	BB75231_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75231	Sample	BB75231	targeted	Absent	Sample	Upin_Bot2011_COI	T4-06	COI	45	BB75231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.34208
BB75232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89955	WGS84	BB75232	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75232_Cjap_Sim2018_COI	T4-08	1	BB75232_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75232	Sample	BB75232	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-08	COI	45	BB75232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33941
BB75232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.89955	WGS84	BB75232	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75232_Mgig_Bot2012_COI	T4-08	1	BB75232_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75232	Sample	BB75232	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-08	COI	45	BB75232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.33941
BB75233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84798	WGS84	BB75233	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75233_Cmae_Bot2011_COI	T4-10	1	BB75233_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75233	Sample	BB75233	targeted	Absent	Sample	Cmae_Bot2011_COI	T4-10	COI	45	BB75233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-10	ATGAACAGTCTATCCTCCTTTAG	147.87403
BB75235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95924	WGS84	BB75235	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.365	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75235_Aamu_Bax2006_COI	T4-14	1	BB75235_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75235	Sample	BB75235	targeted	Present	Sample	Aamu_Bax2006_COI	T4-14	COI	45	BB75235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-14	GCACAACCGGGATCTTTACTTC	147.52587
BB75235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95924	WGS84	BB75235	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75235_Pcan_Bot2011_IGS	T4-14	1	BB75235_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75235	Sample	BB75235	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-14	IGS	45	BB75235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-14	CGTAATCCTCAGTACTGGCTA	147.52587
BB75235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95924	WGS84	BB75235	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75235_Sspa_Oph2007_COI	T4-14	1	BB75235_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75235	Sample	BB75235	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-14	COI	45	BB75235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-14	GAAGGCCTTCCGATGATC	147.52587
BB75235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95924	WGS84	BB75235	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75235_Upin_Bot2011_COI	T4-14	1	BB75235_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75235	Sample	BB75235	targeted	Absent	Sample	Upin_Bot2011_COI	T4-14	COI	45	BB75235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.52587
BB75236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82928	WGS84	BB75236	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75236_Cjap_Sim2018_COI	T4-01	1	BB75236_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75236	Sample	BB75236	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-01	COI	45	BB75236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30265
BB75236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82928	WGS84	BB75236	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75236_Mgig_Bot2012_COI	T4-01	1	BB75236_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75236	Sample	BB75236	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-01	COI	45	BB75236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.30265
BB75237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82524	WGS84	BB75237	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.47	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75237_Cmae_Bot2011_COI	T4-03	1	BB75237_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75237	Sample	BB75237	targeted	Present	Sample	Cmae_Bot2011_COI	T4-03	COI	45	BB75237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-03	ATGAACAGTCTATCCTCCTTTAG	147.30752
BB75239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89951	WGS84	BB75239	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.865	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75239_Aamu_Bax2006_COI	T4-07	1	BB75239_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75239	Sample	BB75239	targeted	Present	Sample	Aamu_Bax2006_COI	T4-07	COI	45	BB75239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-07	GCACAACCGGGATCTTTACTTC	147.33637
BB75239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89951	WGS84	BB75239	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75239_Pcan_Bot2011_IGS	T4-07	1	BB75239_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75239	Sample	BB75239	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-07	IGS	45	BB75239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-07	CGTAATCCTCAGTACTGGCTA	147.33637
BB75239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89951	WGS84	BB75239	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75239_Sspa_Oph2007_COI	T4-07	1	BB75239_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75239	Sample	BB75239	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-07	COI	45	BB75239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-07	GAAGGCCTTCCGATGATC	147.33637
BB75239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89951	WGS84	BB75239	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75239_Upin_Bot2011_COI	T4-07	1	BB75239_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75239	Sample	BB75239	targeted	Absent	Sample	Upin_Bot2011_COI	T4-07	COI	45	BB75239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.33637
BB75240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88548	WGS84	BB75240	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75240_Cmae_Bot2011_COI	T4-09	1	BB75240_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75240	Sample	BB75240	targeted	Absent	Sample	Cmae_Bot2011_COI	T4-09	COI	45	BB75240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-09	ATGAACAGTCTATCCTCCTTTAG	147.33615
BB75242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96131	WGS84	BB75242	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.694	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75242_Aamu_Bax2006_COI	T4-13	1	BB75242_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75242	Sample	BB75242	targeted	Present	Sample	Aamu_Bax2006_COI	T4-13	COI	45	BB75242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-13	GCACAACCGGGATCTTTACTTC	147.53343
BB75242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96131	WGS84	BB75242	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75242_Pcan_Bot2011_IGS	T4-13	1	BB75242_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75242	Sample	BB75242	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-13	IGS	45	BB75242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-13	CGTAATCCTCAGTACTGGCTA	147.53343
BB75242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96131	WGS84	BB75242	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75242_Sspa_Oph2007_COI	T4-13	1	BB75242_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75242	Sample	BB75242	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-13	COI	45	BB75242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-13	GAAGGCCTTCCGATGATC	147.53343
BB75242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96131	WGS84	BB75242	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75242_Upin_Bot2011_COI	T4-13	1	BB75242_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75242	Sample	BB75242	targeted	Absent	Sample	Upin_Bot2011_COI	T4-13	COI	45	BB75242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.53343
BB75243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96162	WGS84	BB75243	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75243_Cjap_Sim2018_COI	T4-15	1	BB75243_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75243	Sample	BB75243	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-15	COI	45	BB75243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51952
BB75243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96162	WGS84	BB75243	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.486	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75243_Mgig_Bot2012_COI	T4-15	1	BB75243_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75243	Sample	BB75243	targeted	Present	Sample	Mgig_Bot2012_COI	T4-15	COI	45	BB75243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.51952
BB75247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81044	WGS84	BB75247	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75247_Cjap_Sim2018_COI	S1-20	1	BB75247_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75247	Sample	BB75247	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-20	COI	45	BB75247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-20	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52108
BB75247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.81044	WGS84	BB75247	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.758	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75247_Mgig_Bot2012_COI	S1-20	1	BB75247_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75247	Sample	BB75247	targeted	Present	Sample	Mgig_Bot2012_COI	S1-20	COI	45	BB75247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-20	TCTTATTCGTTGGAGACTTTATAACCCT	138.52108
BB75248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80931	WGS84	BB75248	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75248_Cmae_Bot2011_COI	S2-20	1	BB75248_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75248	Sample	BB75248	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-20	COI	45	BB75248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-20	ATGAACAGTCTATCCTCCTTTAG	138.5177
BB80076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.7824	WGS84	BB80076	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80076_Cjap_Sim2018_COI	S4-01	1	BB80076_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80076	Sample	BB80076	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-01	COI	45	BB80076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51709
BB80076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.7824	WGS84	BB80076	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.728	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80076_Mgig_Bot2012_COI	S4-01	1	BB80076_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80076	Sample	BB80076	targeted	Present	Sample	Mgig_Bot2012_COI	S4-01	COI	45	BB80076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.51709
BB80077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78295	WGS84	BB80077	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80077_Cmae_Bot2011_COI	S4-02	1	BB80077_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80077	Sample	BB80077	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-02	COI	45	BB80077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-02	ATGAACAGTCTATCCTCCTTTAG	138.51697
BB80082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84439	WGS84	BB80082	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80082_Aamu_Bax2006_COI	S4-07	1	BB80082_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80082	Sample	BB80082	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-07	COI	45	BB80082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-07	GCACAACCGGGATCTTTACTTC	138.49743
BB80082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84439	WGS84	BB80082	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80082_Pcan_Bot2011_IGS	S4-07	1	BB80082_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80082	Sample	BB80082	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-07	IGS	45	BB80082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-07	CGTAATCCTCAGTACTGGCTA	138.49743
BB80082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84439	WGS84	BB80082	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80082_Sspa_Oph2007_COI	S4-07	1	BB80082_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80082	Sample	BB80082	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-07	COI	45	BB80082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-07	GAAGGCCTTCCGATGATC	138.49743
BB80082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84439	WGS84	BB80082	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80082_Upin_Bot2011_COI	S4-07	1	BB80082_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80082	Sample	BB80082	targeted	Absent	Sample	Upin_Bot2011_COI	S4-07	COI	45	BB80082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.49743
BB80084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84516	WGS84	BB80084	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80084_Cmae_Bot2011_COI	S4-08	1	BB80084_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80084	Sample	BB80084	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-08	COI	45	BB80084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-08	ATGAACAGTCTATCCTCCTTTAG	138.49722
BB80087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83727	WGS84	BB80087	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80087_Cjap_Sim2018_COI	S4-11	1	BB80087_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80087	Sample	BB80087	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-11	COI	45	BB80087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50732
BB80087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83727	WGS84	BB80087	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.894	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80087_Mgig_Bot2012_COI	S4-11	1	BB80087_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80087	Sample	BB80087	targeted	Present	Sample	Mgig_Bot2012_COI	S4-11	COI	45	BB80087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.50732
BB80088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8367	WGS84	BB80088	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80088_Cmae_Bot2011_COI	S4-12	1	BB80088_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80088	Sample	BB80088	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-12	COI	45	BB80088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-12	ATGAACAGTCTATCCTCCTTTAG	138.50862
BB80106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78368	WGS84	BB80106	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80106_Cjap_Sim2018_COI	S3-20	1	BB80106_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80106	Sample	BB80106	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-20	COI	45	BB80106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-20	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5164
BB80106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78368	WGS84	BB80106	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.035	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80106_Mgig_Bot2012_COI	S3-20	1	BB80106_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80106	Sample	BB80106	targeted	Present	Sample	Mgig_Bot2012_COI	S3-20	COI	45	BB80106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-20	TCTTATTCGTTGGAGACTTTATAACCCT	138.5164
BB80107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78497	WGS84	BB80107	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.855	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80107_Cmae_Bot2011_COI	S3-19	1	BB80107_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80107	Sample	BB80107	targeted	Present	Sample	Cmae_Bot2011_COI	S3-19	COI	45	BB80107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-19	ATGAACAGTCTATCCTCCTTTAG	138.51619
BB80109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78732	WGS84	BB80109	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80109_Aamu_Bax2006_COI	S3-17	1	BB80109_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80109	Sample	BB80109	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-17	COI	45	BB80109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-17	GCACAACCGGGATCTTTACTTC	138.51553
BB80109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78732	WGS84	BB80109	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80109_Pcan_Bot2011_IGS	S3-17	1	BB80109_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80109	Sample	BB80109	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-17	IGS	45	BB80109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-17	CGTAATCCTCAGTACTGGCTA	138.51553
BB80109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78732	WGS84	BB80109	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80109_Sspa_Oph2007_COI	S3-17	1	BB80109_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80109	Sample	BB80109	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-17	COI	45	BB80109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-17	GAAGGCCTTCCGATGATC	138.51553
BB80109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78732	WGS84	BB80109	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80109_Upin_Bot2011_COI	S3-17	1	BB80109_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80109	Sample	BB80109	targeted	Absent	Sample	Upin_Bot2011_COI	S3-17	COI	45	BB80109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.51553
BB80110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78837	WGS84	BB80110	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80110_Cmae_Bot2011_COI	S3-16	1	BB80110_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80110	Sample	BB80110	targeted	Absent	Sample	Cmae_Bot2011_COI	S3-16	COI	45	BB80110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-16	ATGAACAGTCTATCCTCCTTTAG	138.51486
BB80112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81082	WGS84	BB80112	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80112_Aamu_Bax2006_COI	S3-14	1	BB80112_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80112	Sample	BB80112	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-14	COI	45	BB80112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-14	GCACAACCGGGATCTTTACTTC	138.52156
BB80112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81082	WGS84	BB80112	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80112_Pcan_Bot2011_IGS	S3-14	1	BB80112_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80112	Sample	BB80112	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-14	IGS	45	BB80112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-14	CGTAATCCTCAGTACTGGCTA	138.52156
BB80112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81082	WGS84	BB80112	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80112_Sspa_Oph2007_COI	S3-14	1	BB80112_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80112	Sample	BB80112	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-14	COI	45	BB80112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-14	GAAGGCCTTCCGATGATC	138.52156
BB80112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81082	WGS84	BB80112	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80112_Upin_Bot2011_COI	S3-14	1	BB80112_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80112	Sample	BB80112	targeted	Absent	Sample	Upin_Bot2011_COI	S3-14	COI	45	BB80112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.52156
BB80113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81015	WGS84	BB80113	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80113_Cjap_Sim2018_COI	S3-13	1	BB80113_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80113	Sample	BB80113	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-13	COI	45	BB80113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52006
BB80113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.81015	WGS84	BB80113	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.326	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80113_Mgig_Bot2012_COI	S3-13	1	BB80113_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80113	Sample	BB80113	targeted	Present	Sample	Mgig_Bot2012_COI	S3-13	COI	45	BB80113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.52006
BB80114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80965	WGS84	BB80114	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80114_Cmae_Bot2011_COI	S3-12	1	BB80114_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80114	Sample	BB80114	targeted	Absent	Sample	Cmae_Bot2011_COI	S3-12	COI	45	BB80114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-12	ATGAACAGTCTATCCTCCTTTAG	138.51882
BB80116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB80116	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80116_Aamu_Bax2006_COI	S3-08	1	BB80116_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80116	Sample	BB80116	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-08	COI	45	BB80116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-08	GCACAACCGGGATCTTTACTTC	138.50858
BB80116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB80116	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80116_Pcan_Bot2011_IGS	S3-08	1	BB80116_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80116	Sample	BB80116	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-08	IGS	45	BB80116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-08	CGTAATCCTCAGTACTGGCTA	138.50858
BB80116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB80116	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80116_Sspa_Oph2007_COI	S3-08	1	BB80116_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80116	Sample	BB80116	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-08	COI	45	BB80116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-08	GAAGGCCTTCCGATGATC	138.50858
BB80116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB80116	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80116_Upin_Bot2011_COI	S3-08	1	BB80116_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80116	Sample	BB80116	targeted	Absent	Sample	Upin_Bot2011_COI	S3-08	COI	45	BB80116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.50858
BB80117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83634	WGS84	BB80117	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80117_Cjap_Sim2018_COI	S3-09	1	BB80117_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80117	Sample	BB80117	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-09	COI	45	BB80117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5092
BB80117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83634	WGS84	BB80117	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.668	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80117_Mgig_Bot2012_COI	S3-09	1	BB80117_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80117	Sample	BB80117	targeted	Present	Sample	Mgig_Bot2012_COI	S3-09	COI	45	BB80117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.5092
BB80118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83573	WGS84	BB80118	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.704	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80118_Cmae_Bot2011_COI	S3-10	1	BB80118_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80118	Sample	BB80118	targeted	Present	Sample	Cmae_Bot2011_COI	S3-10	COI	45	BB80118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-10	ATGAACAGTCTATCCTCCTTTAG	138.50796
BB80120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83819	WGS84	BB80120	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80120_Cjap_Sim2018_COI	S3-06	1	BB80120_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80120	Sample	BB80120	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-06	COI	45	BB80120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50691
BB80120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83819	WGS84	BB80120	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.24	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80120_Mgig_Bot2012_COI	S3-06	1	BB80120_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80120	Sample	BB80120	targeted	Present	Sample	Mgig_Bot2012_COI	S3-06	COI	45	BB80120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.50691
BB80121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84305	WGS84	BB80121	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.489	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80121_Cmae_Bot2011_COI	S3-05	1	BB80121_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80121	Sample	BB80121	targeted	Present	Sample	Cmae_Bot2011_COI	S3-05	COI	45	BB80121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-05	ATGAACAGTCTATCCTCCTTTAG	138.49947
BB80123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84423	WGS84	BB80123	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80123_Aamu_Bax2006_COI	S3-03	1	BB80123_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80123	Sample	BB80123	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-03	COI	45	BB80123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-03	GCACAACCGGGATCTTTACTTC	138.49776
BB80123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84423	WGS84	BB80123	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80123_Pcan_Bot2011_IGS	S3-03	1	BB80123_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80123	Sample	BB80123	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-03	IGS	45	BB80123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-03	CGTAATCCTCAGTACTGGCTA	138.49776
BB80123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84423	WGS84	BB80123	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80123_Sspa_Oph2007_COI	S3-03	1	BB80123_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80123	Sample	BB80123	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-03	COI	45	BB80123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-03	GAAGGCCTTCCGATGATC	138.49776
BB80123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84423	WGS84	BB80123	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80123_Upin_Bot2011_COI	S3-03	1	BB80123_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80123	Sample	BB80123	targeted	Absent	Sample	Upin_Bot2011_COI	S3-03	COI	45	BB80123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.49776
BB80124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8452	WGS84	BB80124	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80124_Cjap_Sim2018_COI	S3-02	1	BB80124_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80124	Sample	BB80124	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-02	COI	45	BB80124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4972
BB80124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.8452	WGS84	BB80124	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.737	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80124_Mgig_Bot2012_COI	S3-02	1	BB80124_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80124	Sample	BB80124	targeted	Present	Sample	Mgig_Bot2012_COI	S3-02	COI	45	BB80124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.4972
BB80125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84665	WGS84	BB80125	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.436	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80125_Cmae_Bot2011_COI	S3-01	1	BB80125_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80125	Sample	BB80125	targeted	Present	Sample	Cmae_Bot2011_COI	S3-01	COI	45	BB80125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-01	ATGAACAGTCTATCCTCCTTTAG	138.49724
BB80145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38774	WGS84	BB80145	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80145_Aamu_Bax2006_COI	BB22	1	BB80145_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80145	Sample	BB80145	targeted	Absent	Sample	Aamu_Bax2006_COI	BB22	COI	45	BB80145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB22	GCACAACCGGGATCTTTACTTC	153.15869
BB80145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38774	WGS84	BB80145	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80145_Pcan_Bot2011_IGS	BB22	1	BB80145_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80145	Sample	BB80145	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB22	IGS	45	BB80145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB22	CGTAATCCTCAGTACTGGCTA	153.15869
BB80145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38774	WGS84	BB80145	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80145_Sspa_Oph2007_COI	BB22	1	BB80145_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80145	Sample	BB80145	targeted	Absent	Sample	Sspa_Oph2007_COI	BB22	COI	45	BB80145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB22	GAAGGCCTTCCGATGATC	153.15869
BB80145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38774	WGS84	BB80145	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80145_Upin_Bot2011_COI	BB22	1	BB80145_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80145	Sample	BB80145	targeted	Absent	Sample	Upin_Bot2011_COI	BB22	COI	45	BB80145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB22	CTTTAATTACAGCGTTTTTATTGTTGT	153.15869
BB80146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35841	WGS84	BB80146	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80146_Mgig_Bot2012_COI	BB2	1	BB80146_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80146	Sample	BB80146	targeted	Absent	Sample	Mgig_Bot2012_COI	BB2	COI	45	BB80146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB2	TCTTATTCGTTGGAGACTTTATAACCCT	153.17941
BB80147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38249	WGS84	BB80147	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80147_Cmae_Bot2011_COI	BB18	1	BB80147_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80147	Sample	BB80147	targeted	Absent	Sample	Cmae_Bot2011_COI	BB18	COI	45	BB80147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB18	ATGAACAGTCTATCCTCCTTTAG	153.16121
BB80147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38249	WGS84	BB80147	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80147_Msal_Bot2012_COI	BB18	1	BB80147_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80147	Sample	BB80147	targeted	Absent	Sample	Msal_Bot2012_COI	BB18	COI	45	BB80147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB18	GYTAGTTCCRATGATGTTAGCTG	153.16121
BB80149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.376	WGS84	BB80149	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80149_Aamu_Bax2006_COI	BB13	1	BB80149_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80149	Sample	BB80149	targeted	Absent	Sample	Aamu_Bax2006_COI	BB13	COI	45	BB80149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB13	GCACAACCGGGATCTTTACTTC	153.16704
BB80149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.376	WGS84	BB80149	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80149_Pcan_Bot2011_IGS	BB13	1	BB80149_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80149	Sample	BB80149	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB13	IGS	45	BB80149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB13	CGTAATCCTCAGTACTGGCTA	153.16704
BB80149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.376	WGS84	BB80149	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80149_Sspa_Oph2007_COI	BB13	1	BB80149_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80149	Sample	BB80149	targeted	Absent	Sample	Sspa_Oph2007_COI	BB13	COI	45	BB80149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB13	GAAGGCCTTCCGATGATC	153.16704
BB80149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.376	WGS84	BB80149	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80149_Upin_Bot2011_COI	BB13	1	BB80149_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80149	Sample	BB80149	targeted	Absent	Sample	Upin_Bot2011_COI	BB13	COI	45	BB80149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB13	CTTTAATTACAGCGTTTTTATTGTTGT	153.16704
BB80150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37228	WGS84	BB80150	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80150_Cmae_Bot2011_COI	BB11	1	BB80150_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80150	Sample	BB80150	targeted	Absent	Sample	Cmae_Bot2011_COI	BB11	COI	45	BB80150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB11	ATGAACAGTCTATCCTCCTTTAG	153.16969
BB80150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37228	WGS84	BB80150	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80150_Msal_Bot2012_COI	BB11	1	BB80150_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80150	Sample	BB80150	targeted	Absent	Sample	Msal_Bot2012_COI	BB11	COI	45	BB80150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB11	GYTAGTTCCRATGATGTTAGCTG	153.16969
BB80152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44756	WGS84	BB80152	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80152_Aamu_Bax2006_COI	BB64	1	BB80152_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80152	Sample	BB80152	targeted	Absent	Sample	Aamu_Bax2006_COI	BB64	COI	45	BB80152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB64	GCACAACCGGGATCTTTACTTC	153.09609
BB80152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44756	WGS84	BB80152	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80152_Pcan_Bot2011_IGS	BB64	1	BB80152_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80152	Sample	BB80152	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB64	IGS	45	BB80152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB64	CGTAATCCTCAGTACTGGCTA	153.09609
BB80152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44756	WGS84	BB80152	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80152_Sspa_Oph2007_COI	BB64	1	BB80152_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80152	Sample	BB80152	targeted	Absent	Sample	Sspa_Oph2007_COI	BB64	COI	45	BB80152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB64	GAAGGCCTTCCGATGATC	153.09609
BB80152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44756	WGS84	BB80152	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80152_Upin_Bot2011_COI	BB64	1	BB80152_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80152	Sample	BB80152	targeted	Absent	Sample	Upin_Bot2011_COI	BB64	COI	45	BB80152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB64	CTTTAATTACAGCGTTTTTATTGTTGT	153.09609
BB80153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44718	WGS84	BB80153	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80153_Mgig_Bot2012_COI	BB63	1	BB80153_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80153	Sample	BB80153	targeted	Absent	Sample	Mgig_Bot2012_COI	BB63	COI	45	BB80153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB63	TCTTATTCGTTGGAGACTTTATAACCCT	153.09844
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80154_Cmae_Bot2011_COI	MB90	1	BB80154_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Absent	Sample	Cmae_Bot2011_COI	MB90	COI	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	ATGAACAGTCTATCCTCCTTTAG	144.91468
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80154_Hsan_Wil2021_COI	MB90	1	BB80154_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Absent	Sample	Hsan_Wil2021_COI	MB90	COI	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	CCTGGGCCGGTATAGTAGGT	144.91468
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80154_Msal_Bot2012_COI	MB90	1	BB80154_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Absent	Sample	Msal_Bot2012_COI	MB90	COI	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	GYTAGTTCCRATGATGTTAGCTG	144.91468
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80156_Aamu_Bax2006_COI	MB80	1	BB80156_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Absent	Sample	Aamu_Bax2006_COI	MB80	COI	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	GCACAACCGGGATCTTTACTTC	144.92768
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80156_Pcan_Bot2011_IGS	MB80	1	BB80156_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB80	IGS	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	CGTAATCCTCAGTACTGGCTA	144.92768
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.66473007	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80156_Sspa_Oph2007_COI	MB80	1	BB80156_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Present	Sample	Sspa_Oph2007_COI	MB80	COI	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	GAAGGCCTTCCGATGATC	144.92768
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80156_Upin_Bot2011_COI	MB80	1	BB80156_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Absent	Sample	Upin_Bot2011_COI	MB80	COI	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	CTTTAATTACAGCGTTTTTATTGTTGT	144.92768
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80157_Mgig_Bot2012_COI	MB65	1	BB80157_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Mgig_Bot2012_COI	MB65	COI	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	TCTTATTCGTTGGAGACTTTATAACCCT	144.922
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80158_Cmae_Bot2011_COI	MB60	1	BB80158_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Absent	Sample	Cmae_Bot2011_COI	MB60	COI	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	ATGAACAGTCTATCCTCCTTTAG	144.90884
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80158_Hsan_Wil2021_COI	MB60	1	BB80158_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Absent	Sample	Hsan_Wil2021_COI	MB60	COI	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	CCTGGGCCGGTATAGTAGGT	144.90884
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80158_Msal_Bot2012_COI	MB60	1	BB80158_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Absent	Sample	Msal_Bot2012_COI	MB60	COI	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	GYTAGTTCCRATGATGTTAGCTG	144.90884
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.466	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80160_Mgig_Bot2012_COI	MB100	1	BB80160_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Present	Sample	Mgig_Bot2012_COI	MB100	COI	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	TCTTATTCGTTGGAGACTTTATAACCCT	144.91317
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80161_Cmae_Bot2011_COI	MB115	1	BB80161_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Absent	Sample	Cmae_Bot2011_COI	MB115	COI	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	ATGAACAGTCTATCCTCCTTTAG	144.90568
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80161_Hsan_Wil2021_COI	MB115	1	BB80161_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Absent	Sample	Hsan_Wil2021_COI	MB115	COI	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	CCTGGGCCGGTATAGTAGGT	144.90568
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80161_Msal_Bot2012_COI	MB115	1	BB80161_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Absent	Sample	Msal_Bot2012_COI	MB115	COI	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	GYTAGTTCCRATGATGTTAGCTG	144.90568
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80163_Aamu_Bax2006_COI	MB50	1	BB80163_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Absent	Sample	Aamu_Bax2006_COI	MB50	COI	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	GCACAACCGGGATCTTTACTTC	144.90737
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80163_Pcan_Bot2011_IGS	MB50	1	BB80163_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB50	IGS	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	CGTAATCCTCAGTACTGGCTA	144.90737
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.12760162	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80163_Sspa_Oph2007_COI	MB50	1	BB80163_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Present	Sample	Sspa_Oph2007_COI	MB50	COI	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	GAAGGCCTTCCGATGATC	144.90737
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	28.514	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80163_Upin_Bot2011_COI	MB50	1	BB80163_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Present	Sample	Upin_Bot2011_COI	MB50	COI	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	CTTTAATTACAGCGTTTTTATTGTTGT	144.90737
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80164_Mgig_Bot2012_COI	MB45	1	BB80164_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Absent	Sample	Mgig_Bot2012_COI	MB45	COI	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	TCTTATTCGTTGGAGACTTTATAACCCT	144.91008
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80165_Cmae_Bot2011_COI	MB40	1	BB80165_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Absent	Sample	Cmae_Bot2011_COI	MB40	COI	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	ATGAACAGTCTATCCTCCTTTAG	144.9135
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80165_Hsan_Wil2021_COI	MB40	1	BB80165_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Absent	Sample	Hsan_Wil2021_COI	MB40	COI	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	CCTGGGCCGGTATAGTAGGT	144.9135
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80165_Msal_Bot2012_COI	MB40	1	BB80165_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Absent	Sample	Msal_Bot2012_COI	MB40	COI	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	GYTAGTTCCRATGATGTTAGCTG	144.9135
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.389	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80167_Aamu_Bax2006_COI	MB30	1	BB80167_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Present	Sample	Aamu_Bax2006_COI	MB30	COI	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	GCACAACCGGGATCTTTACTTC	144.91604
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80167_Pcan_Bot2011_IGS	MB30	1	BB80167_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB30	IGS	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	CGTAATCCTCAGTACTGGCTA	144.91604
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.43805695	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80167_Sspa_Oph2007_COI	MB30	1	BB80167_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Present	Sample	Sspa_Oph2007_COI	MB30	COI	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	GAAGGCCTTCCGATGATC	144.91604
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.126	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80167_Upin_Bot2011_COI	MB30	1	BB80167_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Present	Sample	Upin_Bot2011_COI	MB30	COI	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	CTTTAATTACAGCGTTTTTATTGTTGT	144.91604
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80168_Mgig_Bot2012_COI	MB5	1	BB80168_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Absent	Sample	Mgig_Bot2012_COI	MB5	COI	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	TCTTATTCGTTGGAGACTTTATAACCCT	144.9048
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80169_Cmae_Bot2011_COI	MB15	1	BB80169_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Absent	Sample	Cmae_Bot2011_COI	MB15	COI	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	ATGAACAGTCTATCCTCCTTTAG	144.90877
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	38.478	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80169_Hsan_Wil2021_COI	MB15	1	BB80169_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Present	Sample	Hsan_Wil2021_COI	MB15	COI	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	CCTGGGCCGGTATAGTAGGT	144.90877
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80169_Msal_Bot2012_COI	MB15	1	BB80169_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Absent	Sample	Msal_Bot2012_COI	MB15	COI	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	GYTAGTTCCRATGATGTTAGCTG	144.90877
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.101	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80170_Aamu_Bax2006_COI	MB10	1	BB80170_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Present	Sample	Aamu_Bax2006_COI	MB10	COI	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	GCACAACCGGGATCTTTACTTC	144.90737
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80170_Pcan_Bot2011_IGS	MB10	1	BB80170_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB10	IGS	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	CGTAATCCTCAGTACTGGCTA	144.90737
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.52951813	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80170_Sspa_Oph2007_COI	MB10	1	BB80170_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Present	Sample	Sspa_Oph2007_COI	MB10	COI	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	GAAGGCCTTCCGATGATC	144.90737
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.008	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80170_Upin_Bot2011_COI	MB10	1	BB80170_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Present	Sample	Upin_Bot2011_COI	MB10	COI	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	CTTTAATTACAGCGTTTTTATTGTTGT	144.90737
BB80171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84287	WGS84	BB80171	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80171_Mgig_Bot2012_COI	MB20	1	BB80171_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80171	Sample	BB80171	targeted	Absent	Sample	Mgig_Bot2012_COI	MB20	COI	45	BB80171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB20	TCTTATTCGTTGGAGACTTTATAACCCT	144.92678
BB80178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81639	WGS84	BB80178	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80178_Aamu_Bax2006_COI	MB104	1	BB80178_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80178	Sample	BB80178	targeted	Absent	Sample	Aamu_Bax2006_COI	MB104	COI	45	BB80178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB104	GCACAACCGGGATCTTTACTTC	144.91286
BB80178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81639	WGS84	BB80178	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80178_Pcan_Bot2011_IGS	MB104	1	BB80178_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80178	Sample	BB80178	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB104	IGS	45	BB80178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB104	CGTAATCCTCAGTACTGGCTA	144.91286
BB80178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81639	WGS84	BB80178	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.95334244	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80178_Sspa_Oph2007_COI	MB104	1	BB80178_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80178	Sample	BB80178	targeted	Present	Sample	Sspa_Oph2007_COI	MB104	COI	45	BB80178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB104	GAAGGCCTTCCGATGATC	144.91286
BB80178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81639	WGS84	BB80178	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80178_Upin_Bot2011_COI	MB104	1	BB80178_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80178	Sample	BB80178	targeted	Absent	Sample	Upin_Bot2011_COI	MB104	COI	45	BB80178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB104	CTTTAATTACAGCGTTTTTATTGTTGT	144.91286
BB80179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81378	WGS84	BB80179	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.316	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80179_Mgig_Bot2012_COI	MB102	1	BB80179_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80179	Sample	BB80179	targeted	Present	Sample	Mgig_Bot2012_COI	MB102	COI	45	BB80179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB102	TCTTATTCGTTGGAGACTTTATAACCCT	144.91311
BB80181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.80956	WGS84	BB80181	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80181_Aamu_Bax2006_COI	MB96	1	BB80181_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80181	Sample	BB80181	targeted	Absent	Sample	Aamu_Bax2006_COI	MB96	COI	45	BB80181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB96	GCACAACCGGGATCTTTACTTC	144.91452
BB80181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.80956	WGS84	BB80181	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80181_Pcan_Bot2011_IGS	MB96	1	BB80181_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80181	Sample	BB80181	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB96	IGS	45	BB80181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB96	CGTAATCCTCAGTACTGGCTA	144.91452
BB80181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.80956	WGS84	BB80181	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.23277664	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80181_Sspa_Oph2007_COI	MB96	1	BB80181_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80181	Sample	BB80181	targeted	Present	Sample	Sspa_Oph2007_COI	MB96	COI	45	BB80181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB96	GAAGGCCTTCCGATGATC	144.91452
BB80181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.80956	WGS84	BB80181	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80181_Upin_Bot2011_COI	MB96	1	BB80181_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80181	Sample	BB80181	targeted	Absent	Sample	Upin_Bot2011_COI	MB96	COI	45	BB80181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB96	CTTTAATTACAGCGTTTTTATTGTTGT	144.91452
BB80182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81211	WGS84	BB80182	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.679	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80182_Mgig_Bot2012_COI	MB94	1	BB80182_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80182	Sample	BB80182	targeted	Present	Sample	Mgig_Bot2012_COI	MB94	COI	45	BB80182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB94	TCTTATTCGTTGGAGACTTTATAACCCT	144.91485
BB80183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81479	WGS84	BB80183	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80183_Cmae_Bot2011_COI	MB92	1	BB80183_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80183	Sample	BB80183	targeted	Absent	Sample	Cmae_Bot2011_COI	MB92	COI	45	BB80183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB92	ATGAACAGTCTATCCTCCTTTAG	144.91433
BB80183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81479	WGS84	BB80183	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80183_Msal_Bot2012_COI	MB92	1	BB80183_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80183	Sample	BB80183	targeted	Absent	Sample	Msal_Bot2012_COI	MB92	COI	45	BB80183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB92	GYTAGTTCCRATGATGTTAGCTG	144.91433
BB80185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81841	WGS84	BB80185	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80185_Aamu_Bax2006_COI	MB87	1	BB80185_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80185	Sample	BB80185	targeted	Absent	Sample	Aamu_Bax2006_COI	MB87	COI	45	BB80185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB87	GCACAACCGGGATCTTTACTTC	144.91788
BB80185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81841	WGS84	BB80185	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80185_Pcan_Bot2011_IGS	MB87	1	BB80185_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80185	Sample	BB80185	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB87	IGS	45	BB80185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB87	CGTAATCCTCAGTACTGGCTA	144.91788
BB80185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81841	WGS84	BB80185	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.28046036	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80185_Sspa_Oph2007_COI	MB87	1	BB80185_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80185	Sample	BB80185	targeted	Present	Sample	Sspa_Oph2007_COI	MB87	COI	45	BB80185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB87	GAAGGCCTTCCGATGATC	144.91788
BB80185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81841	WGS84	BB80185	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80185_Upin_Bot2011_COI	MB87	1	BB80185_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80185	Sample	BB80185	targeted	Absent	Sample	Upin_Bot2011_COI	MB87	COI	45	BB80185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB87	CTTTAATTACAGCGTTTTTATTGTTGT	144.91788
BB80186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81617	WGS84	BB80186	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80186_Mgig_Bot2012_COI	MB83	1	BB80186_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80186	Sample	BB80186	targeted	Absent	Sample	Mgig_Bot2012_COI	MB83	COI	45	BB80186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB83	TCTTATTCGTTGGAGACTTTATAACCCT	144.92378
BB80187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81514	WGS84	BB80187	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80187_Cmae_Bot2011_COI	MB81	1	BB80187_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80187	Sample	BB80187	targeted	Absent	Sample	Cmae_Bot2011_COI	MB81	COI	45	BB80187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB81	ATGAACAGTCTATCCTCCTTTAG	144.92658
BB80187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81514	WGS84	BB80187	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80187_Msal_Bot2012_COI	MB81	1	BB80187_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80187	Sample	BB80187	targeted	Absent	Sample	Msal_Bot2012_COI	MB81	COI	45	BB80187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB81	GYTAGTTCCRATGATGTTAGCTG	144.92658
BB80189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81818	WGS84	BB80189	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80189_Aamu_Bax2006_COI	MB76	1	BB80189_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80189	Sample	BB80189	targeted	Absent	Sample	Aamu_Bax2006_COI	MB76	COI	45	BB80189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB76	GCACAACCGGGATCTTTACTTC	144.92699
BB80189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81818	WGS84	BB80189	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80189_Pcan_Bot2011_IGS	MB76	1	BB80189_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80189	Sample	BB80189	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB76	IGS	45	BB80189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB76	CGTAATCCTCAGTACTGGCTA	144.92699
BB80189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81818	WGS84	BB80189	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.32675934	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80189_Sspa_Oph2007_COI	MB76	1	BB80189_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80189	Sample	BB80189	targeted	Present	Sample	Sspa_Oph2007_COI	MB76	COI	45	BB80189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB76	GAAGGCCTTCCGATGATC	144.92699
BB80189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81818	WGS84	BB80189	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80189_Upin_Bot2011_COI	MB76	1	BB80189_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80189	Sample	BB80189	targeted	Absent	Sample	Upin_Bot2011_COI	MB76	COI	45	BB80189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB76	CTTTAATTACAGCGTTTTTATTGTTGT	144.92699
BB80190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81812	WGS84	BB80190	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80190_Cmae_Bot2011_COI	MB73	1	BB80190_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80190	Sample	BB80190	targeted	Absent	Sample	Cmae_Bot2011_COI	MB73	COI	45	BB80190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB73	ATGAACAGTCTATCCTCCTTTAG	144.93004
BB80190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81812	WGS84	BB80190	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80190_Msal_Bot2012_COI	MB73	1	BB80190_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80190	Sample	BB80190	targeted	Absent	Sample	Msal_Bot2012_COI	MB73	COI	45	BB80190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB73	GYTAGTTCCRATGATGTTAGCTG	144.93004
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81889	WGS84	BB80192	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80192_Aamu_Bax2006_COI	MB71	1	BB80192_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Absent	Sample	Aamu_Bax2006_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	GCACAACCGGGATCTTTACTTC	144.93166
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81889	WGS84	BB80192	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80192_Pcan_Bot2011_IGS	MB71	1	BB80192_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB71	IGS	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	CGTAATCCTCAGTACTGGCTA	144.93166
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81889	WGS84	BB80192	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.55024338	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80192_Sspa_Oph2007_COI	MB71	1	BB80192_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Present	Sample	Sspa_Oph2007_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	GAAGGCCTTCCGATGATC	144.93166
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81889	WGS84	BB80192	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80192_Upin_Bot2011_COI	MB71	1	BB80192_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Absent	Sample	Upin_Bot2011_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	CTTTAATTACAGCGTTTTTATTGTTGT	144.93166
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81889	WGS84	BB80192	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80192_Vgib_Wil2025_COI	MB71	1	BB80192_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Absent	Sample	Vgib_Wil2025_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	CCCCTCTTTACAAGTTCTTTAGGGT	144.93166
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82058	WGS84	BB80193	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.223	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80193_Asen_Wil2025_COI	MB69	1	BB80193_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Present	Sample	Asen_Wil2025_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	GTTGTTGTTACAACACATGCTCTTTTG	144.93081
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82058	WGS84	BB80193	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	38.796	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80193_Mgig_Bot2012_COI	MB69	1	BB80193_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Present	Sample	Mgig_Bot2012_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	TCTTATTCGTTGGAGACTTTATAACCCT	144.93081
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82022	WGS84	BB80194	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80194_Cmae_Bot2011_COI	MB67	1	BB80194_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Cmae_Bot2011_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	ATGAACAGTCTATCCTCCTTTAG	144.92791
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82022	WGS84	BB80194	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80194_Msal_Bot2012_COI	MB67	1	BB80194_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Msal_Bot2012_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	GYTAGTTCCRATGATGTTAGCTG	144.92791
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81986	WGS84	BB80196	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80196_Aamu_Bax2006_COI	MB66	1	BB80196_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Aamu_Bax2006_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	GCACAACCGGGATCTTTACTTC	144.9239
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81986	WGS84	BB80196	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80196_Pcan_Bot2011_IGS	MB66	1	BB80196_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB66	IGS	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	CGTAATCCTCAGTACTGGCTA	144.9239
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81986	WGS84	BB80196	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80196_Sspa_Oph2007_COI	MB66	1	BB80196_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Sspa_Oph2007_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	GAAGGCCTTCCGATGATC	144.9239
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81986	WGS84	BB80196	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80196_Upin_Bot2011_COI	MB66	1	BB80196_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Upin_Bot2011_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	CTTTAATTACAGCGTTTTTATTGTTGT	144.9239
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81986	WGS84	BB80196	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80196_Vgib_Wil2025_COI	MB66	1	BB80196_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Vgib_Wil2025_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	CCCCTCTTTACAAGTTCTTTAGGGT	144.9239
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82016	WGS84	BB80197	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.875	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80197_Asen_Wil2025_COI	MB63	1	BB80197_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Present	Sample	Asen_Wil2025_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	GTTGTTGTTACAACACATGCTCTTTTG	144.91788
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82016	WGS84	BB80197	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.064	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80197_Mgig_Bot2012_COI	MB63	1	BB80197_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Present	Sample	Mgig_Bot2012_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	TCTTATTCGTTGGAGACTTTATAACCCT	144.91788
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82068	WGS84	BB80198	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80198_Cmae_Bot2011_COI	MB62	1	BB80198_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Absent	Sample	Cmae_Bot2011_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	ATGAACAGTCTATCCTCCTTTAG	144.91443
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82068	WGS84	BB80198	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80198_Msal_Bot2012_COI	MB62	1	BB80198_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Absent	Sample	Msal_Bot2012_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	GYTAGTTCCRATGATGTTAGCTG	144.91443
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82492	WGS84	BB80200	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80200_Aamu_Bax2006_COI	MB58	1	BB80200_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Absent	Sample	Aamu_Bax2006_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	GCACAACCGGGATCTTTACTTC	144.90413
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82492	WGS84	BB80200	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80200_Pcan_Bot2011_IGS	MB58	1	BB80200_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB58	IGS	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	CGTAATCCTCAGTACTGGCTA	144.90413
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82492	WGS84	BB80200	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.71141815	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80200_Sspa_Oph2007_COI	MB58	1	BB80200_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Present	Sample	Sspa_Oph2007_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	GAAGGCCTTCCGATGATC	144.90413
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82492	WGS84	BB80200	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80200_Upin_Bot2011_COI	MB58	1	BB80200_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Absent	Sample	Upin_Bot2011_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	CTTTAATTACAGCGTTTTTATTGTTGT	144.90413
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82492	WGS84	BB80200	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80200_Vgib_Wil2025_COI	MB58	1	BB80200_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Absent	Sample	Vgib_Wil2025_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	CCCCTCTTTACAAGTTCTTTAGGGT	144.90413
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82853	WGS84	BB80201	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.137	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80201_Asen_Wil2025_COI	MB56	1	BB80201_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Present	Sample	Asen_Wil2025_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	GTTGTTGTTACAACACATGCTCTTTTG	144.90063
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82853	WGS84	BB80201	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80201_Mgig_Bot2012_COI	MB56	1	BB80201_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Absent	Sample	Mgig_Bot2012_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	TCTTATTCGTTGGAGACTTTATAACCCT	144.90063
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82694	WGS84	BB80202	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80202_Cmae_Bot2011_COI	MB126	1	BB80202_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Absent	Sample	Cmae_Bot2011_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	ATGAACAGTCTATCCTCCTTTAG	144.89947
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82694	WGS84	BB80202	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80202_Msal_Bot2012_COI	MB126	1	BB80202_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Absent	Sample	Msal_Bot2012_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	GYTAGTTCCRATGATGTTAGCTG	144.89947
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82329	WGS84	BB80204	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80204_Aamu_Bax2006_COI	MB121	1	BB80204_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Absent	Sample	Aamu_Bax2006_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	GCACAACCGGGATCTTTACTTC	144.90234
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82329	WGS84	BB80204	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80204_Pcan_Bot2011_IGS	MB121	1	BB80204_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB121	IGS	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	CGTAATCCTCAGTACTGGCTA	144.90234
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82329	WGS84	BB80204	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.78598785	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80204_Sspa_Oph2007_COI	MB121	1	BB80204_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Present	Sample	Sspa_Oph2007_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	GAAGGCCTTCCGATGATC	144.90234
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82329	WGS84	BB80204	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80204_Upin_Bot2011_COI	MB121	1	BB80204_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Absent	Sample	Upin_Bot2011_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	CTTTAATTACAGCGTTTTTATTGTTGT	144.90234
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82329	WGS84	BB80204	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80204_Vgib_Wil2025_COI	MB121	1	BB80204_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Absent	Sample	Vgib_Wil2025_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	CCCCTCTTTACAAGTTCTTTAGGGT	144.90234
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82145	WGS84	BB80205	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.592	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80205_Asen_Wil2025_COI	MB119	1	BB80205_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Present	Sample	Asen_Wil2025_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	GTTGTTGTTACAACACATGCTCTTTTG	144.90501
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82145	WGS84	BB80205	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.459	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80205_Mgig_Bot2012_COI	MB119	1	BB80205_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Present	Sample	Mgig_Bot2012_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	TCTTATTCGTTGGAGACTTTATAACCCT	144.90501
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81765	WGS84	BB80206	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80206_Cmae_Bot2011_COI	MB116	1	BB80206_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Absent	Sample	Cmae_Bot2011_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	ATGAACAGTCTATCCTCCTTTAG	144.9054
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81765	WGS84	BB80206	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80206_Msal_Bot2012_COI	MB116	1	BB80206_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Absent	Sample	Msal_Bot2012_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	GYTAGTTCCRATGATGTTAGCTG	144.9054
BB80208	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81853	WGS84	BB80208	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80208_Aamu_Bax2006_COI	MB112	1	BB80208_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80208	Sample	BB80208	targeted	Absent	Sample	Aamu_Bax2006_COI	MB112	COI	45	BB80208	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB112	GCACAACCGGGATCTTTACTTC	144.90678
BB80208	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81853	WGS84	BB80208	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80208_Pcan_Bot2011_IGS	MB112	1	BB80208_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80208	Sample	BB80208	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB112	IGS	45	BB80208	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB112	CGTAATCCTCAGTACTGGCTA	144.90678
BB80208	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81853	WGS84	BB80208	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.99620056	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80208_Sspa_Oph2007_COI	MB112	1	BB80208_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80208	Sample	BB80208	targeted	Present	Sample	Sspa_Oph2007_COI	MB112	COI	45	BB80208	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB112	GAAGGCCTTCCGATGATC	144.90678
BB80208	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81853	WGS84	BB80208	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80208_Upin_Bot2011_COI	MB112	1	BB80208_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80208	Sample	BB80208	targeted	Absent	Sample	Upin_Bot2011_COI	MB112	COI	45	BB80208	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB112	CTTTAATTACAGCGTTTTTATTGTTGT	144.90678
BB80209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85495	WGS84	BB80209	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80209_Mgig_Bot2012_COI	MB54	1	BB80209_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80209	Sample	BB80209	targeted	Absent	Sample	Mgig_Bot2012_COI	MB54	COI	45	BB80209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB54	TCTTATTCGTTGGAGACTTTATAACCCT	144.90386
BB80211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.861	WGS84	BB80211	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.804	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80211_Aamu_Bax2006_COI	MB49	1	BB80211_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80211	Sample	BB80211	targeted	Present	Sample	Aamu_Bax2006_COI	MB49	COI	45	BB80211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB49	GCACAACCGGGATCTTTACTTC	144.90817
BB80211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.861	WGS84	BB80211	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80211_Pcan_Bot2011_IGS	MB49	1	BB80211_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80211	Sample	BB80211	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB49	IGS	45	BB80211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB49	CGTAATCCTCAGTACTGGCTA	144.90817
BB80211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.861	WGS84	BB80211	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.95139694	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80211_Sspa_Oph2007_COI	MB49	1	BB80211_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80211	Sample	BB80211	targeted	Present	Sample	Sspa_Oph2007_COI	MB49	COI	45	BB80211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB49	GAAGGCCTTCCGATGATC	144.90817
BB80211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.861	WGS84	BB80211	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.629	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80211_Upin_Bot2011_COI	MB49	1	BB80211_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80211	Sample	BB80211	targeted	Present	Sample	Upin_Bot2011_COI	MB49	COI	45	BB80211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB49	CTTTAATTACAGCGTTTTTATTGTTGT	144.90817
BB80212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86015	WGS84	BB80212	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.794	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80212_Mgig_Bot2012_COI	MB47	1	BB80212_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80212	Sample	BB80212	targeted	Present	Sample	Mgig_Bot2012_COI	MB47	COI	45	BB80212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB47	TCTTATTCGTTGGAGACTTTATAACCCT	144.90951
BB80213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86038	WGS84	BB80213	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80213_Cmae_Bot2011_COI	MB46	1	BB80213_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80213	Sample	BB80213	targeted	Absent	Sample	Cmae_Bot2011_COI	MB46	COI	45	BB80213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB46	ATGAACAGTCTATCCTCCTTTAG	144.91026
BB80213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86038	WGS84	BB80213	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80213_Msal_Bot2012_COI	MB46	1	BB80213_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80213	Sample	BB80213	targeted	Absent	Sample	Msal_Bot2012_COI	MB46	COI	45	BB80213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB46	GYTAGTTCCRATGATGTTAGCTG	144.91026
BB80215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86302	WGS84	BB80215	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.267	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80215_Aamu_Bax2006_COI	MB39	1	BB80215_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80215	Sample	BB80215	targeted	Present	Sample	Aamu_Bax2006_COI	MB39	COI	45	BB80215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB39	GCACAACCGGGATCTTTACTTC	144.9144
BB80215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86302	WGS84	BB80215	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80215_Pcan_Bot2011_IGS	MB39	1	BB80215_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80215	Sample	BB80215	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB39	IGS	45	BB80215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB39	CGTAATCCTCAGTACTGGCTA	144.9144
BB80215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86302	WGS84	BB80215	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	44.96103287	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80215_Sspa_Oph2007_COI	MB39	1	BB80215_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80215	Sample	BB80215	targeted	Present	Sample	Sspa_Oph2007_COI	MB39	COI	45	BB80215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB39	GAAGGCCTTCCGATGATC	144.9144
BB80215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86302	WGS84	BB80215	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.675	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80215_Upin_Bot2011_COI	MB39	1	BB80215_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80215	Sample	BB80215	targeted	Present	Sample	Upin_Bot2011_COI	MB39	COI	45	BB80215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB39	CTTTAATTACAGCGTTTTTATTGTTGT	144.9144
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80216_Mgig_Bot2012_COI	MB38	1	BB80216_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Absent	Sample	Mgig_Bot2012_COI	MB38	COI	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	TCTTATTCGTTGGAGACTTTATAACCCT	144.91498
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80217_Cmae_Bot2011_COI	MB33	1	BB80217_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Absent	Sample	Cmae_Bot2011_COI	MB33	COI	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	ATGAACAGTCTATCCTCCTTTAG	144.91683
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80217_Hsan_Wil2021_COI	MB33	1	BB80217_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Absent	Sample	Hsan_Wil2021_COI	MB33	COI	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	CCTGGGCCGGTATAGTAGGT	144.91683
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80217_Msal_Bot2012_COI	MB33	1	BB80217_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Absent	Sample	Msal_Bot2012_COI	MB33	COI	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	GYTAGTTCCRATGATGTTAGCTG	144.91683
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.112	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80219_Aamu_Bax2006_COI	MB2	1	BB80219_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Present	Sample	Aamu_Bax2006_COI	MB2	COI	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	GCACAACCGGGATCTTTACTTC	144.90381
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80219_Pcan_Bot2011_IGS	MB2	1	BB80219_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB2	IGS	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	CGTAATCCTCAGTACTGGCTA	144.90381
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.52187729	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80219_Sspa_Oph2007_COI	MB2	1	BB80219_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Present	Sample	Sspa_Oph2007_COI	MB2	COI	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	GAAGGCCTTCCGATGATC	144.90381
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.889	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80219_Upin_Bot2011_COI	MB2	1	BB80219_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Present	Sample	Upin_Bot2011_COI	MB2	COI	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	CTTTAATTACAGCGTTTTTATTGTTGT	144.90381
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80220_Cmae_Bot2011_COI	MB9	1	BB80220_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Absent	Sample	Cmae_Bot2011_COI	MB9	COI	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	ATGAACAGTCTATCCTCCTTTAG	144.90576
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80220_Hsan_Wil2021_COI	MB9	1	BB80220_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Absent	Sample	Hsan_Wil2021_COI	MB9	COI	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	CCTGGGCCGGTATAGTAGGT	144.90576
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80220_Msal_Bot2012_COI	MB9	1	BB80220_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Absent	Sample	Msal_Bot2012_COI	MB9	COI	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	GYTAGTTCCRATGATGTTAGCTG	144.90576
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.577	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80222_Aamu_Bax2006_COI	MB16	1	BB80222_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Present	Sample	Aamu_Bax2006_COI	MB16	COI	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	GCACAACCGGGATCTTTACTTC	144.91447
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80222_Pcan_Bot2011_IGS	MB16	1	BB80222_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB16	IGS	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	CGTAATCCTCAGTACTGGCTA	144.91447
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.14080048	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80222_Sspa_Oph2007_COI	MB16	1	BB80222_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Present	Sample	Sspa_Oph2007_COI	MB16	COI	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	GAAGGCCTTCCGATGATC	144.91447
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.368	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80222_Upin_Bot2011_COI	MB16	1	BB80222_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Present	Sample	Upin_Bot2011_COI	MB16	COI	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	CTTTAATTACAGCGTTTTTATTGTTGT	144.91447
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80223_Mgig_Bot2012_COI	MB13	1	BB80223_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Absent	Sample	Mgig_Bot2012_COI	MB13	COI	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	TCTTATTCGTTGGAGACTTTATAACCCT	144.91033
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80224_Cmae_Bot2011_COI	MB12	1	BB80224_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Absent	Sample	Cmae_Bot2011_COI	MB12	COI	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	ATGAACAGTCTATCCTCCTTTAG	144.90642
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80224_Hsan_Wil2021_COI	MB12	1	BB80224_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Absent	Sample	Hsan_Wil2021_COI	MB12	COI	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	CCTGGGCCGGTATAGTAGGT	144.90642
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80224_Msal_Bot2012_COI	MB12	1	BB80224_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Absent	Sample	Msal_Bot2012_COI	MB12	COI	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	GYTAGTTCCRATGATGTTAGCTG	144.90642
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.864	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80226_Aamu_Bax2006_COI	MB28	1	BB80226_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Present	Sample	Aamu_Bax2006_COI	MB28	COI	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	GCACAACCGGGATCTTTACTTC	144.9324
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80226_Pcan_Bot2011_IGS	MB28	1	BB80226_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB28	IGS	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	CGTAATCCTCAGTACTGGCTA	144.9324
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.97726822	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80226_Sspa_Oph2007_COI	MB28	1	BB80226_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Present	Sample	Sspa_Oph2007_COI	MB28	COI	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	GAAGGCCTTCCGATGATC	144.9324
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.272	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80226_Upin_Bot2011_COI	MB28	1	BB80226_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Present	Sample	Upin_Bot2011_COI	MB28	COI	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	CTTTAATTACAGCGTTTTTATTGTTGT	144.9324
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80227_Mgig_Bot2012_COI	MB26	1	BB80227_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Absent	Sample	Mgig_Bot2012_COI	MB26	COI	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	TCTTATTCGTTGGAGACTTTATAACCCT	144.92895
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80228_Cmae_Bot2011_COI	MB23	1	BB80228_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Absent	Sample	Cmae_Bot2011_COI	MB23	COI	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	ATGAACAGTCTATCCTCCTTTAG	144.92963
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.887	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80228_Hsan_Wil2021_COI	MB23	1	BB80228_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Present	Sample	Hsan_Wil2021_COI	MB23	COI	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	CCTGGGCCGGTATAGTAGGT	144.92963
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80228_Msal_Bot2012_COI	MB23	1	BB80228_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Absent	Sample	Msal_Bot2012_COI	MB23	COI	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	GYTAGTTCCRATGATGTTAGCTG	144.92963
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80230_Mgig_Bot2012_COI	MB18	1	BB80230_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Absent	Sample	Mgig_Bot2012_COI	MB18	COI	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	TCTTATTCGTTGGAGACTTTATAACCCT	144.92605
BB80231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84499	WGS84	BB80231	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.324	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80231_Cmae_Bot2011_COI	HB20	1	BB80231_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80231	Sample	BB80231	targeted	Present	Sample	Cmae_Bot2011_COI	HB20	COI	45	BB80231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB20	ATGAACAGTCTATCCTCCTTTAG	147.32948
BB80231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84499	WGS84	BB80231	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80231_Msal_Bot2012_COI	HB20	1	BB80231_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80231	Sample	BB80231	targeted	Absent	Sample	Msal_Bot2012_COI	HB20	COI	45	BB80231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB20	GYTAGTTCCRATGATGTTAGCTG	147.32948
BB80233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88499	WGS84	BB80233	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.456	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80233_Aamu_Bax2006_COI	HB5	1	BB80233_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80233	Sample	BB80233	targeted	Present	Sample	Aamu_Bax2006_COI	HB5	COI	45	BB80233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB5	GCACAACCGGGATCTTTACTTC	147.33665
BB80233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88499	WGS84	BB80233	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80233_Pcan_Bot2011_IGS	HB5	1	BB80233_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80233	Sample	BB80233	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB5	IGS	45	BB80233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB5	CGTAATCCTCAGTACTGGCTA	147.33665
BB80233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88499	WGS84	BB80233	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80233_Sspa_Oph2007_COI	HB5	1	BB80233_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80233	Sample	BB80233	targeted	Absent	Sample	Sspa_Oph2007_COI	HB5	COI	45	BB80233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB5	GAAGGCCTTCCGATGATC	147.33665
BB80233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88499	WGS84	BB80233	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80233_Upin_Bot2011_COI	HB5	1	BB80233_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80233	Sample	BB80233	targeted	Absent	Sample	Upin_Bot2011_COI	HB5	COI	45	BB80233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB5	CTTTAATTACAGCGTTTTTATTGTTGT	147.33665
BB80239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88538	WGS84	BB80239	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80239_Cmae_Bot2011_COI	HB9	1	BB80239_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80239	Sample	BB80239	targeted	Absent	Sample	Cmae_Bot2011_COI	HB9	COI	45	BB80239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB9	ATGAACAGTCTATCCTCCTTTAG	147.33553
BB80239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88538	WGS84	BB80239	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80239_Msal_Bot2012_COI	HB9	1	BB80239_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80239	Sample	BB80239	targeted	Absent	Sample	Msal_Bot2012_COI	HB9	COI	45	BB80239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB9	GYTAGTTCCRATGATGTTAGCTG	147.33553
BB80240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.885	WGS84	BB80240	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.792	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80240_Aamu_Bax2006_COI	HB8	1	BB80240_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80240	Sample	BB80240	targeted	Present	Sample	Aamu_Bax2006_COI	HB8	COI	45	BB80240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB8	GCACAACCGGGATCTTTACTTC	147.33265
BB80240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.885	WGS84	BB80240	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80240_Pcan_Bot2011_IGS	HB8	1	BB80240_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80240	Sample	BB80240	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB8	IGS	45	BB80240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB8	CGTAATCCTCAGTACTGGCTA	147.33265
BB80240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.885	WGS84	BB80240	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80240_Sspa_Oph2007_COI	HB8	1	BB80240_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80240	Sample	BB80240	targeted	Absent	Sample	Sspa_Oph2007_COI	HB8	COI	45	BB80240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB8	GAAGGCCTTCCGATGATC	147.33265
BB80240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.885	WGS84	BB80240	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80240_Upin_Bot2011_COI	HB8	1	BB80240_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80240	Sample	BB80240	targeted	Absent	Sample	Upin_Bot2011_COI	HB8	COI	45	BB80240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB8	CTTTAATTACAGCGTTTTTATTGTTGT	147.33265
BB80241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88368	WGS84	BB80241	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.73	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80241_Mgig_Bot2012_COI	HB7	1	BB80241_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80241	Sample	BB80241	targeted	Present	Sample	Mgig_Bot2012_COI	HB7	COI	45	BB80241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB7	TCTTATTCGTTGGAGACTTTATAACCCT	147.33443
BB80242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88453	WGS84	BB80242	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80242_Cmae_Bot2011_COI	HB6	1	BB80242_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80242	Sample	BB80242	targeted	Absent	Sample	Cmae_Bot2011_COI	HB6	COI	45	BB80242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB6	ATGAACAGTCTATCCTCCTTTAG	147.33358
BB80242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88453	WGS84	BB80242	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80242_Msal_Bot2012_COI	HB6	1	BB80242_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80242	Sample	BB80242	targeted	Absent	Sample	Msal_Bot2012_COI	HB6	COI	45	BB80242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB6	GYTAGTTCCRATGATGTTAGCTG	147.33358
BB80244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88586	WGS84	BB80244	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.203	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80244_Aamu_Bax2006_COI	HB1	1	BB80244_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80244	Sample	BB80244	targeted	Present	Sample	Aamu_Bax2006_COI	HB1	COI	45	BB80244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB1	GCACAACCGGGATCTTTACTTC	147.33887
BB80244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88586	WGS84	BB80244	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80244_Pcan_Bot2011_IGS	HB1	1	BB80244_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80244	Sample	BB80244	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB1	IGS	45	BB80244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB1	CGTAATCCTCAGTACTGGCTA	147.33887
BB80244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88586	WGS84	BB80244	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80244_Sspa_Oph2007_COI	HB1	1	BB80244_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80244	Sample	BB80244	targeted	Absent	Sample	Sspa_Oph2007_COI	HB1	COI	45	BB80244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB1	GAAGGCCTTCCGATGATC	147.33887
BB80244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88586	WGS84	BB80244	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80244_Upin_Bot2011_COI	HB1	1	BB80244_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80244	Sample	BB80244	targeted	Absent	Sample	Upin_Bot2011_COI	HB1	COI	45	BB80244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB1	CTTTAATTACAGCGTTTTTATTGTTGT	147.33887
BB80245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84503	WGS84	BB80245	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80245_Mgig_Bot2012_COI	HB21	1	BB80245_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80245	Sample	BB80245	targeted	Absent	Sample	Mgig_Bot2012_COI	HB21	COI	45	BB80245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB21	TCTTATTCGTTGGAGACTTTATAACCCT	147.32826
BB80246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84502	WGS84	BB80246	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.728	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80246_Cmae_Bot2011_COI	HB18	1	BB80246_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80246	Sample	BB80246	targeted	Present	Sample	Cmae_Bot2011_COI	HB18	COI	45	BB80246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB18	ATGAACAGTCTATCCTCCTTTAG	147.33073
BB80246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84502	WGS84	BB80246	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80246_Msal_Bot2012_COI	HB18	1	BB80246_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80246	Sample	BB80246	targeted	Absent	Sample	Msal_Bot2012_COI	HB18	COI	45	BB80246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB18	GYTAGTTCCRATGATGTTAGCTG	147.33073
BB80248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84752	WGS84	BB80248	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.17	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80248_Aamu_Bax2006_COI	HB14	1	BB80248_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80248	Sample	BB80248	targeted	Present	Sample	Aamu_Bax2006_COI	HB14	COI	45	BB80248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB14	GCACAACCGGGATCTTTACTTC	147.32763
BB80248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84752	WGS84	BB80248	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80248_Pcan_Bot2011_IGS	HB14	1	BB80248_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80248	Sample	BB80248	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB14	IGS	45	BB80248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB14	CGTAATCCTCAGTACTGGCTA	147.32763
BB80248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84752	WGS84	BB80248	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80248_Sspa_Oph2007_COI	HB14	1	BB80248_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80248	Sample	BB80248	targeted	Absent	Sample	Sspa_Oph2007_COI	HB14	COI	45	BB80248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB14	GAAGGCCTTCCGATGATC	147.32763
BB80248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84752	WGS84	BB80248	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80248_Upin_Bot2011_COI	HB14	1	BB80248_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80248	Sample	BB80248	targeted	Absent	Sample	Upin_Bot2011_COI	HB14	COI	45	BB80248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB14	CTTTAATTACAGCGTTTTTATTGTTGT	147.32763
BB80249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.83215	WGS84	BB80249	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80249_Mgig_Bot2012_COI	HB24	1	BB80249_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80249	Sample	BB80249	targeted	Absent	Sample	Mgig_Bot2012_COI	HB24	COI	45	BB80249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB24	TCTTATTCGTTGGAGACTTTATAACCCT	147.32207
BB80251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81897	WGS84	BB80251	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80251_Aamu_Bax2006_COI	GB110	1	BB80251_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80251	Sample	BB80251	targeted	Absent	Sample	Aamu_Bax2006_COI	GB110	COI	45	BB80251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB110	GCACAACCGGGATCTTTACTTC	151.23877
BB80251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81897	WGS84	BB80251	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80251_Pcan_Bot2011_IGS	GB110	1	BB80251_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80251	Sample	BB80251	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB110	IGS	45	BB80251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB110	CGTAATCCTCAGTACTGGCTA	151.23877
BB80251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81897	WGS84	BB80251	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80251_Sspa_Oph2007_COI	GB110	1	BB80251_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80251	Sample	BB80251	targeted	Absent	Sample	Sspa_Oph2007_COI	GB110	COI	45	BB80251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB110	GAAGGCCTTCCGATGATC	151.23877
BB80251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81897	WGS84	BB80251	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80251_Upin_Bot2011_COI	GB110	1	BB80251_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80251	Sample	BB80251	targeted	Absent	Sample	Upin_Bot2011_COI	GB110	COI	45	BB80251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB110	CTTTAATTACAGCGTTTTTATTGTTGT	151.23877
BB80252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79283	WGS84	BB80252	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80252_Mgig_Bot2012_COI	GB95	1	BB80252_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80252	Sample	BB80252	targeted	Absent	Sample	Mgig_Bot2012_COI	GB95	COI	45	BB80252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB95	TCTTATTCGTTGGAGACTTTATAACCCT	151.21146
BB80253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79251	WGS84	BB80253	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80253_Cmae_Bot2011_COI	GB60	1	BB80253_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80253	Sample	BB80253	targeted	Absent	Sample	Cmae_Bot2011_COI	GB60	COI	45	BB80253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB60	ATGAACAGTCTATCCTCCTTTAG	151.18214
BB80253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79251	WGS84	BB80253	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80253_Msal_Bot2012_COI	GB60	1	BB80253_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80253	Sample	BB80253	targeted	Absent	Sample	Msal_Bot2012_COI	GB60	COI	45	BB80253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB60	GYTAGTTCCRATGATGTTAGCTG	151.18214
BB80255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77796	WGS84	BB80255	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80255_Aamu_Bax2006_COI	GB85	1	BB80255_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80255	Sample	BB80255	targeted	Absent	Sample	Aamu_Bax2006_COI	GB85	COI	45	BB80255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB85	GCACAACCGGGATCTTTACTTC	151.19555
BB80255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77796	WGS84	BB80255	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80255_Pcan_Bot2011_IGS	GB85	1	BB80255_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80255	Sample	BB80255	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB85	IGS	45	BB80255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB85	CGTAATCCTCAGTACTGGCTA	151.19555
BB80255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77796	WGS84	BB80255	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80255_Sspa_Oph2007_COI	GB85	1	BB80255_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80255	Sample	BB80255	targeted	Absent	Sample	Sspa_Oph2007_COI	GB85	COI	45	BB80255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB85	GAAGGCCTTCCGATGATC	151.19555
BB80255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77796	WGS84	BB80255	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80255_Upin_Bot2011_COI	GB85	1	BB80255_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80255	Sample	BB80255	targeted	Absent	Sample	Upin_Bot2011_COI	GB85	COI	45	BB80255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB85	CTTTAATTACAGCGTTTTTATTGTTGT	151.19555
BB80256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81467	WGS84	BB80256	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80256_Mgig_Bot2012_COI	GB80	1	BB80256_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80256	Sample	BB80256	targeted	Absent	Sample	Mgig_Bot2012_COI	GB80	COI	45	BB80256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB80	TCTTATTCGTTGGAGACTTTATAACCCT	151.23642
BB80257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82328	WGS84	BB80257	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80257_Cmae_Bot2011_COI	GB70	1	BB80257_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80257	Sample	BB80257	targeted	Absent	Sample	Cmae_Bot2011_COI	GB70	COI	45	BB80257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB70	ATGAACAGTCTATCCTCCTTTAG	151.24379
BB80257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82328	WGS84	BB80257	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80257_Msal_Bot2012_COI	GB70	1	BB80257_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80257	Sample	BB80257	targeted	Absent	Sample	Msal_Bot2012_COI	GB70	COI	45	BB80257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB70	GYTAGTTCCRATGATGTTAGCTG	151.24379
BB80259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.7849	WGS84	BB80259	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80259_Aamu_Bax2006_COI	GB45	1	BB80259_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80259	Sample	BB80259	targeted	Absent	Sample	Aamu_Bax2006_COI	GB45	COI	45	BB80259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB45	GCACAACCGGGATCTTTACTTC	151.17149
BB80259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.7849	WGS84	BB80259	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80259_Pcan_Bot2011_IGS	GB45	1	BB80259_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80259	Sample	BB80259	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB45	IGS	45	BB80259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB45	CGTAATCCTCAGTACTGGCTA	151.17149
BB80259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.7849	WGS84	BB80259	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80259_Sspa_Oph2007_COI	GB45	1	BB80259_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80259	Sample	BB80259	targeted	Absent	Sample	Sspa_Oph2007_COI	GB45	COI	45	BB80259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB45	GAAGGCCTTCCGATGATC	151.17149
BB80259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.7849	WGS84	BB80259	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80259_Upin_Bot2011_COI	GB45	1	BB80259_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80259	Sample	BB80259	targeted	Absent	Sample	Upin_Bot2011_COI	GB45	COI	45	BB80259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB45	CTTTAATTACAGCGTTTTTATTGTTGT	151.17149
BB80260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80815	WGS84	BB80260	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80260_Cmae_Bot2011_COI	GB40	1	BB80260_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80260	Sample	BB80260	targeted	Absent	Sample	Cmae_Bot2011_COI	GB40	COI	45	BB80260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB40	ATGAACAGTCTATCCTCCTTTAG	151.21644
BB80260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80815	WGS84	BB80260	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80260_Msal_Bot2012_COI	GB40	1	BB80260_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80260	Sample	BB80260	targeted	Absent	Sample	Msal_Bot2012_COI	GB40	COI	45	BB80260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB40	GYTAGTTCCRATGATGTTAGCTG	151.21644
BB80262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83508	WGS84	BB80262	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80262_Aamu_Bax2006_COI	GB20	1	BB80262_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80262	Sample	BB80262	targeted	Absent	Sample	Aamu_Bax2006_COI	GB20	COI	45	BB80262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB20	GCACAACCGGGATCTTTACTTC	151.26929
BB80262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83508	WGS84	BB80262	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80262_Pcan_Bot2011_IGS	GB20	1	BB80262_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80262	Sample	BB80262	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB20	IGS	45	BB80262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB20	CGTAATCCTCAGTACTGGCTA	151.26929
BB80262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83508	WGS84	BB80262	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80262_Sspa_Oph2007_COI	GB20	1	BB80262_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80262	Sample	BB80262	targeted	Absent	Sample	Sspa_Oph2007_COI	GB20	COI	45	BB80262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB20	GAAGGCCTTCCGATGATC	151.26929
BB80262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83508	WGS84	BB80262	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80262_Upin_Bot2011_COI	GB20	1	BB80262_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80262	Sample	BB80262	targeted	Absent	Sample	Upin_Bot2011_COI	GB20	COI	45	BB80262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB20	CTTTAATTACAGCGTTTTTATTGTTGT	151.26929
BB80263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8365	WGS84	BB80263	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80263_Mgig_Bot2012_COI	GB15	1	BB80263_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80263	Sample	BB80263	targeted	Absent	Sample	Mgig_Bot2012_COI	GB15	COI	45	BB80263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB15	TCTTATTCGTTGGAGACTTTATAACCCT	151.2728
BB80264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85116	WGS84	BB80264	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80264_Cmae_Bot2011_COI	GB10	1	BB80264_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80264	Sample	BB80264	targeted	Absent	Sample	Cmae_Bot2011_COI	GB10	COI	45	BB80264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB10	ATGAACAGTCTATCCTCCTTTAG	151.31425
BB80264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85116	WGS84	BB80264	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80264_Msal_Bot2012_COI	GB10	1	BB80264_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80264	Sample	BB80264	targeted	Absent	Sample	Msal_Bot2012_COI	GB10	COI	45	BB80264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB10	GYTAGTTCCRATGATGTTAGCTG	151.31425
BB80271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80759	WGS84	BB80271	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80271_Cmae_Bot2011_COI	GB43	1	BB80271_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80271	Sample	BB80271	targeted	Absent	Sample	Cmae_Bot2011_COI	GB43	COI	45	BB80271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB43	ATGAACAGTCTATCCTCCTTTAG	151.22026
BB80271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80759	WGS84	BB80271	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80271_Msal_Bot2012_COI	GB43	1	BB80271_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80271	Sample	BB80271	targeted	Absent	Sample	Msal_Bot2012_COI	GB43	COI	45	BB80271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB43	GYTAGTTCCRATGATGTTAGCTG	151.22026
BB80273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80713	WGS84	BB80273	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80273_Aamu_Bax2006_COI	GB38	1	BB80273_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80273	Sample	BB80273	targeted	Absent	Sample	Aamu_Bax2006_COI	GB38	COI	45	BB80273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB38	GCACAACCGGGATCTTTACTTC	151.21483
BB80273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80713	WGS84	BB80273	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80273_Pcan_Bot2011_IGS	GB38	1	BB80273_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80273	Sample	BB80273	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB38	IGS	45	BB80273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB38	CGTAATCCTCAGTACTGGCTA	151.21483
BB80273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80713	WGS84	BB80273	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80273_Sspa_Oph2007_COI	GB38	1	BB80273_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80273	Sample	BB80273	targeted	Absent	Sample	Sspa_Oph2007_COI	GB38	COI	45	BB80273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB38	GAAGGCCTTCCGATGATC	151.21483
BB80273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80713	WGS84	BB80273	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80273_Upin_Bot2011_COI	GB38	1	BB80273_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80273	Sample	BB80273	targeted	Absent	Sample	Upin_Bot2011_COI	GB38	COI	45	BB80273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB38	CTTTAATTACAGCGTTTTTATTGTTGT	151.21483
BB80274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80665	WGS84	BB80274	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80274_Mgig_Bot2012_COI	GB37	1	BB80274_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80274	Sample	BB80274	targeted	Absent	Sample	Mgig_Bot2012_COI	GB37	COI	45	BB80274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB37	TCTTATTCGTTGGAGACTTTATAACCCT	151.2207
BB80275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79118	WGS84	BB80275	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80275_Cmae_Bot2011_COI	GB63	1	BB80275_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80275	Sample	BB80275	targeted	Absent	Sample	Cmae_Bot2011_COI	GB63	COI	45	BB80275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB63	ATGAACAGTCTATCCTCCTTTAG	151.17737
BB80275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79118	WGS84	BB80275	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80275_Msal_Bot2012_COI	GB63	1	BB80275_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80275	Sample	BB80275	targeted	Absent	Sample	Msal_Bot2012_COI	GB63	COI	45	BB80275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB63	GYTAGTTCCRATGATGTTAGCTG	151.17737
BB80277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78985	WGS84	BB80277	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80277_Aamu_Bax2006_COI	GB57	1	BB80277_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80277	Sample	BB80277	targeted	Absent	Sample	Aamu_Bax2006_COI	GB57	COI	45	BB80277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB57	GCACAACCGGGATCTTTACTTC	151.18016
BB80277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78985	WGS84	BB80277	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80277_Pcan_Bot2011_IGS	GB57	1	BB80277_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80277	Sample	BB80277	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB57	IGS	45	BB80277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB57	CGTAATCCTCAGTACTGGCTA	151.18016
BB80277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78985	WGS84	BB80277	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80277_Sspa_Oph2007_COI	GB57	1	BB80277_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80277	Sample	BB80277	targeted	Absent	Sample	Sspa_Oph2007_COI	GB57	COI	45	BB80277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB57	GAAGGCCTTCCGATGATC	151.18016
BB80277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78985	WGS84	BB80277	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80277_Upin_Bot2011_COI	GB57	1	BB80277_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80277	Sample	BB80277	targeted	Absent	Sample	Upin_Bot2011_COI	GB57	COI	45	BB80277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB57	CTTTAATTACAGCGTTTTTATTGTTGT	151.18016
BB80278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78795	WGS84	BB80278	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80278_Mgig_Bot2012_COI	GB56	1	BB80278_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80278	Sample	BB80278	targeted	Absent	Sample	Mgig_Bot2012_COI	GB56	COI	45	BB80278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB56	TCTTATTCGTTGGAGACTTTATAACCCT	151.17782
BB80279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78885	WGS84	BB80279	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80279_Cmae_Bot2011_COI	GB54	1	BB80279_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80279	Sample	BB80279	targeted	Absent	Sample	Cmae_Bot2011_COI	GB54	COI	45	BB80279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB54	ATGAACAGTCTATCCTCCTTTAG	151.17702
BB80279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78885	WGS84	BB80279	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80279_Msal_Bot2012_COI	GB54	1	BB80279_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80279	Sample	BB80279	targeted	Absent	Sample	Msal_Bot2012_COI	GB54	COI	45	BB80279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB54	GYTAGTTCCRATGATGTTAGCTG	151.17702
BB80280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78708	WGS84	BB80280	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80280_Aamu_Bax2006_COI	GB52	1	BB80280_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80280	Sample	BB80280	targeted	Absent	Sample	Aamu_Bax2006_COI	GB52	COI	45	BB80280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB52	GCACAACCGGGATCTTTACTTC	151.17666
BB80280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78708	WGS84	BB80280	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80280_Pcan_Bot2011_IGS	GB52	1	BB80280_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80280	Sample	BB80280	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB52	IGS	45	BB80280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB52	CGTAATCCTCAGTACTGGCTA	151.17666
BB80280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78708	WGS84	BB80280	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80280_Sspa_Oph2007_COI	GB52	1	BB80280_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80280	Sample	BB80280	targeted	Absent	Sample	Sspa_Oph2007_COI	GB52	COI	45	BB80280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB52	GAAGGCCTTCCGATGATC	151.17666
BB80280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78708	WGS84	BB80280	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80280_Upin_Bot2011_COI	GB52	1	BB80280_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80280	Sample	BB80280	targeted	Absent	Sample	Upin_Bot2011_COI	GB52	COI	45	BB80280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB52	CTTTAATTACAGCGTTTTTATTGTTGT	151.17666
BB80281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78569	WGS84	BB80281	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80281_Mgig_Bot2012_COI	GB48	1	BB80281_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80281	Sample	BB80281	targeted	Absent	Sample	Mgig_Bot2012_COI	GB48	COI	45	BB80281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB48	TCTTATTCGTTGGAGACTTTATAACCCT	151.17473
BB80282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80282	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80282_Cmae_Bot2011_COI	GB46	1	BB80282_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80282	Sample	BB80282	targeted	Absent	Sample	Cmae_Bot2011_COI	GB46	COI	45	BB80282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB46	ATGAACAGTCTATCCTCCTTTAG	151.17273
BB80282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80282	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80282_Msal_Bot2012_COI	GB46	1	BB80282_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80282	Sample	BB80282	targeted	Absent	Sample	Msal_Bot2012_COI	GB46	COI	45	BB80282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB46	GYTAGTTCCRATGATGTTAGCTG	151.17273
BB80284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78078	WGS84	BB80284	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80284_Aamu_Bax2006_COI	GB89	1	BB80284_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80284	Sample	BB80284	targeted	Absent	Sample	Aamu_Bax2006_COI	GB89	COI	45	BB80284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB89	GCACAACCGGGATCTTTACTTC	151.19976
BB80284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78078	WGS84	BB80284	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80284_Pcan_Bot2011_IGS	GB89	1	BB80284_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80284	Sample	BB80284	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB89	IGS	45	BB80284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB89	CGTAATCCTCAGTACTGGCTA	151.19976
BB80284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78078	WGS84	BB80284	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80284_Sspa_Oph2007_COI	GB89	1	BB80284_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80284	Sample	BB80284	targeted	Absent	Sample	Sspa_Oph2007_COI	GB89	COI	45	BB80284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB89	GAAGGCCTTCCGATGATC	151.19976
BB80284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78078	WGS84	BB80284	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80284_Upin_Bot2011_COI	GB89	1	BB80284_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80284	Sample	BB80284	targeted	Absent	Sample	Upin_Bot2011_COI	GB89	COI	45	BB80284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB89	CTTTAATTACAGCGTTTTTATTGTTGT	151.19976
BB80285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77979	WGS84	BB80285	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80285_Mgig_Bot2012_COI	GB87	1	BB80285_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80285	Sample	BB80285	targeted	Absent	Sample	Mgig_Bot2012_COI	GB87	COI	45	BB80285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB87	TCTTATTCGTTGGAGACTTTATAACCCT	151.19725
BB80286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81603	WGS84	BB80286	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80286_Cmae_Bot2011_COI	GB82	1	BB80286_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80286	Sample	BB80286	targeted	Absent	Sample	Cmae_Bot2011_COI	GB82	COI	45	BB80286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB82	ATGAACAGTCTATCCTCCTTTAG	151.2366
BB80286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81603	WGS84	BB80286	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80286_Msal_Bot2012_COI	GB82	1	BB80286_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80286	Sample	BB80286	targeted	Absent	Sample	Msal_Bot2012_COI	GB82	COI	45	BB80286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB82	GYTAGTTCCRATGATGTTAGCTG	151.2366
BB80288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82325	WGS84	BB80288	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80288_Aamu_Bax2006_COI	GB98	1	BB80288_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80288	Sample	BB80288	targeted	Absent	Sample	Aamu_Bax2006_COI	GB98	COI	45	BB80288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB98	GCACAACCGGGATCTTTACTTC	151.24116
BB80288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82325	WGS84	BB80288	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80288_Pcan_Bot2011_IGS	GB98	1	BB80288_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80288	Sample	BB80288	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB98	IGS	45	BB80288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB98	CGTAATCCTCAGTACTGGCTA	151.24116
BB80288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82325	WGS84	BB80288	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80288_Sspa_Oph2007_COI	GB98	1	BB80288_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80288	Sample	BB80288	targeted	Absent	Sample	Sspa_Oph2007_COI	GB98	COI	45	BB80288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB98	GAAGGCCTTCCGATGATC	151.24116
BB80288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82325	WGS84	BB80288	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80288_Upin_Bot2011_COI	GB98	1	BB80288_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80288	Sample	BB80288	targeted	Absent	Sample	Upin_Bot2011_COI	GB98	COI	45	BB80288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB98	CTTTAATTACAGCGTTTTTATTGTTGT	151.24116
BB80289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81519	WGS84	BB80289	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80289_Mgig_Bot2012_COI	GB79	1	BB80289_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80289	Sample	BB80289	targeted	Absent	Sample	Mgig_Bot2012_COI	GB79	COI	45	BB80289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB79	TCTTATTCGTTGGAGACTTTATAACCCT	151.23887
BB80291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82048	WGS84	BB80291	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80291_Aamu_Bax2006_COI	GB76	1	BB80291_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80291	Sample	BB80291	targeted	Absent	Sample	Aamu_Bax2006_COI	GB76	COI	45	BB80291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB76	GCACAACCGGGATCTTTACTTC	151.24452
BB80291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82048	WGS84	BB80291	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80291_Pcan_Bot2011_IGS	GB76	1	BB80291_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80291	Sample	BB80291	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB76	IGS	45	BB80291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB76	CGTAATCCTCAGTACTGGCTA	151.24452
BB80291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82048	WGS84	BB80291	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80291_Sspa_Oph2007_COI	GB76	1	BB80291_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80291	Sample	BB80291	targeted	Absent	Sample	Sspa_Oph2007_COI	GB76	COI	45	BB80291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB76	GAAGGCCTTCCGATGATC	151.24452
BB80291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82048	WGS84	BB80291	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80291_Upin_Bot2011_COI	GB76	1	BB80291_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80291	Sample	BB80291	targeted	Absent	Sample	Upin_Bot2011_COI	GB76	COI	45	BB80291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB76	CTTTAATTACAGCGTTTTTATTGTTGT	151.24452
BB80292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82116	WGS84	BB80292	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80292_Mgig_Bot2012_COI	GB74	1	BB80292_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80292	Sample	BB80292	targeted	Absent	Sample	Mgig_Bot2012_COI	GB74	COI	45	BB80292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB74	TCTTATTCGTTGGAGACTTTATAACCCT	151.24266
BB80293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82236	WGS84	BB80293	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80293_Cmae_Bot2011_COI	GB73	1	BB80293_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80293	Sample	BB80293	targeted	Absent	Sample	Cmae_Bot2011_COI	GB73	COI	45	BB80293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB73	ATGAACAGTCTATCCTCCTTTAG	151.24253
BB80293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82236	WGS84	BB80293	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80293_Msal_Bot2012_COI	GB73	1	BB80293_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80293	Sample	BB80293	targeted	Absent	Sample	Msal_Bot2012_COI	GB73	COI	45	BB80293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB73	GYTAGTTCCRATGATGTTAGCTG	151.24253
BB80295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8244	WGS84	BB80295	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80295_Aamu_Bax2006_COI	GB68	1	BB80295_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80295	Sample	BB80295	targeted	Absent	Sample	Aamu_Bax2006_COI	GB68	COI	45	BB80295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB68	GCACAACCGGGATCTTTACTTC	151.24366
BB80295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8244	WGS84	BB80295	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80295_Pcan_Bot2011_IGS	GB68	1	BB80295_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80295	Sample	BB80295	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB68	IGS	45	BB80295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB68	CGTAATCCTCAGTACTGGCTA	151.24366
BB80295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8244	WGS84	BB80295	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80295_Sspa_Oph2007_COI	GB68	1	BB80295_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80295	Sample	BB80295	targeted	Absent	Sample	Sspa_Oph2007_COI	GB68	COI	45	BB80295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB68	GAAGGCCTTCCGATGATC	151.24366
BB80295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8244	WGS84	BB80295	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80295_Upin_Bot2011_COI	GB68	1	BB80295_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80295	Sample	BB80295	targeted	Absent	Sample	Upin_Bot2011_COI	GB68	COI	45	BB80295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB68	CTTTAATTACAGCGTTTTTATTGTTGT	151.24366
BB80296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82437	WGS84	BB80296	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80296_Mgig_Bot2012_COI	GB66	1	BB80296_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80296	Sample	BB80296	targeted	Absent	Sample	Mgig_Bot2012_COI	GB66	COI	45	BB80296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB66	TCTTATTCGTTGGAGACTTTATAACCCT	151.24506
BB80297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82526	WGS84	BB80297	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80297_Cmae_Bot2011_COI	GB64	1	BB80297_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80297	Sample	BB80297	targeted	Absent	Sample	Cmae_Bot2011_COI	GB64	COI	45	BB80297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB64	ATGAACAGTCTATCCTCCTTTAG	151.24567
BB80297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82526	WGS84	BB80297	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80297_Msal_Bot2012_COI	GB64	1	BB80297_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80297	Sample	BB80297	targeted	Absent	Sample	Msal_Bot2012_COI	GB64	COI	45	BB80297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB64	GYTAGTTCCRATGATGTTAGCTG	151.24567
BB80299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82251	WGS84	BB80299	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80299_Aamu_Bax2006_COI	GB33	1	BB80299_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80299	Sample	BB80299	targeted	Absent	Sample	Aamu_Bax2006_COI	GB33	COI	45	BB80299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB33	GCACAACCGGGATCTTTACTTC	151.24644
BB80299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82251	WGS84	BB80299	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80299_Pcan_Bot2011_IGS	GB33	1	BB80299_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80299	Sample	BB80299	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB33	IGS	45	BB80299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB33	CGTAATCCTCAGTACTGGCTA	151.24644
BB80299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82251	WGS84	BB80299	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80299_Sspa_Oph2007_COI	GB33	1	BB80299_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80299	Sample	BB80299	targeted	Absent	Sample	Sspa_Oph2007_COI	GB33	COI	45	BB80299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB33	GAAGGCCTTCCGATGATC	151.24644
BB80299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82251	WGS84	BB80299	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80299_Upin_Bot2011_COI	GB33	1	BB80299_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80299	Sample	BB80299	targeted	Absent	Sample	Upin_Bot2011_COI	GB33	COI	45	BB80299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB33	CTTTAATTACAGCGTTTTTATTGTTGT	151.24644
BB80300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82617	WGS84	BB80300	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80300_Mgig_Bot2012_COI	GB31	1	BB80300_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80300	Sample	BB80300	targeted	Absent	Sample	Mgig_Bot2012_COI	GB31	COI	45	BB80300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB31	TCTTATTCGTTGGAGACTTTATAACCCT	151.24826
BB80301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82699	WGS84	BB80301	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80301_Cmae_Bot2011_COI	GB29	1	BB80301_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80301	Sample	BB80301	targeted	Absent	Sample	Cmae_Bot2011_COI	GB29	COI	45	BB80301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB29	ATGAACAGTCTATCCTCCTTTAG	151.24773
BB80301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82699	WGS84	BB80301	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80301_Msal_Bot2012_COI	GB29	1	BB80301_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80301	Sample	BB80301	targeted	Absent	Sample	Msal_Bot2012_COI	GB29	COI	45	BB80301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB29	GYTAGTTCCRATGATGTTAGCTG	151.24773
BB80303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82008	WGS84	BB80303	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80303_Aamu_Bax2006_COI	GB107	1	BB80303_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80303	Sample	BB80303	targeted	Absent	Sample	Aamu_Bax2006_COI	GB107	COI	45	BB80303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB107	GCACAACCGGGATCTTTACTTC	151.23915
BB80303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82008	WGS84	BB80303	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80303_Pcan_Bot2011_IGS	GB107	1	BB80303_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80303	Sample	BB80303	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB107	IGS	45	BB80303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB107	CGTAATCCTCAGTACTGGCTA	151.23915
BB80303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82008	WGS84	BB80303	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80303_Sspa_Oph2007_COI	GB107	1	BB80303_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80303	Sample	BB80303	targeted	Absent	Sample	Sspa_Oph2007_COI	GB107	COI	45	BB80303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB107	GAAGGCCTTCCGATGATC	151.23915
BB80303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82008	WGS84	BB80303	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80303_Upin_Bot2011_COI	GB107	1	BB80303_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80303	Sample	BB80303	targeted	Absent	Sample	Upin_Bot2011_COI	GB107	COI	45	BB80303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB107	CTTTAATTACAGCGTTTTTATTGTTGT	151.23915
BB80304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82071	WGS84	BB80304	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80304_Mgig_Bot2012_COI	GB104	1	BB80304_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80304	Sample	BB80304	targeted	Absent	Sample	Mgig_Bot2012_COI	GB104	COI	45	BB80304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB104	TCTTATTCGTTGGAGACTTTATAACCCT	151.2411
BB80305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81982	WGS84	BB80305	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80305_Cmae_Bot2011_COI	GB103	1	BB80305_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80305	Sample	BB80305	targeted	Absent	Sample	Cmae_Bot2011_COI	GB103	COI	45	BB80305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB103	ATGAACAGTCTATCCTCCTTTAG	151.24166
BB80305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81982	WGS84	BB80305	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80305_Msal_Bot2012_COI	GB103	1	BB80305_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80305	Sample	BB80305	targeted	Absent	Sample	Msal_Bot2012_COI	GB103	COI	45	BB80305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB103	GYTAGTTCCRATGATGTTAGCTG	151.24166
BB80307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79354	WGS84	BB80307	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80307_Aamu_Bax2006_COI	GB96	1	BB80307_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80307	Sample	BB80307	targeted	Absent	Sample	Aamu_Bax2006_COI	GB96	COI	45	BB80307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB96	GCACAACCGGGATCTTTACTTC	151.21271
BB80307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79354	WGS84	BB80307	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80307_Pcan_Bot2011_IGS	GB96	1	BB80307_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80307	Sample	BB80307	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB96	IGS	45	BB80307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB96	CGTAATCCTCAGTACTGGCTA	151.21271
BB80307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79354	WGS84	BB80307	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80307_Sspa_Oph2007_COI	GB96	1	BB80307_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80307	Sample	BB80307	targeted	Absent	Sample	Sspa_Oph2007_COI	GB96	COI	45	BB80307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB96	GAAGGCCTTCCGATGATC	151.21271
BB80307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79354	WGS84	BB80307	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80307_Upin_Bot2011_COI	GB96	1	BB80307_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80307	Sample	BB80307	targeted	Absent	Sample	Upin_Bot2011_COI	GB96	COI	45	BB80307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB96	CTTTAATTACAGCGTTTTTATTGTTGT	151.21271
BB80308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79074	WGS84	BB80308	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80308_Mgig_Bot2012_COI	GB93	1	BB80308_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80308	Sample	BB80308	targeted	Absent	Sample	Mgig_Bot2012_COI	GB93	COI	45	BB80308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB93	TCTTATTCGTTGGAGACTTTATAACCCT	151.21098
BB80309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83073	WGS84	BB80309	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80309_Cmae_Bot2011_COI	GB28	1	BB80309_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80309	Sample	BB80309	targeted	Absent	Sample	Cmae_Bot2011_COI	GB28	COI	45	BB80309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB28	ATGAACAGTCTATCCTCCTTTAG	151.25527
BB80309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83073	WGS84	BB80309	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80309_Msal_Bot2012_COI	GB28	1	BB80309_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80309	Sample	BB80309	targeted	Absent	Sample	Msal_Bot2012_COI	GB28	COI	45	BB80309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB28	GYTAGTTCCRATGATGTTAGCTG	151.25527
BB80310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83125	WGS84	BB80310	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80310_Aamu_Bax2006_COI	GB27	1	BB80310_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80310	Sample	BB80310	targeted	Absent	Sample	Aamu_Bax2006_COI	GB27	COI	45	BB80310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB27	GCACAACCGGGATCTTTACTTC	151.25757
BB80310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83125	WGS84	BB80310	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80310_Pcan_Bot2011_IGS	GB27	1	BB80310_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80310	Sample	BB80310	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB27	IGS	45	BB80310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB27	CGTAATCCTCAGTACTGGCTA	151.25757
BB80310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83125	WGS84	BB80310	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80310_Sspa_Oph2007_COI	GB27	1	BB80310_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80310	Sample	BB80310	targeted	Absent	Sample	Sspa_Oph2007_COI	GB27	COI	45	BB80310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB27	GAAGGCCTTCCGATGATC	151.25757
BB80310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83125	WGS84	BB80310	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80310_Upin_Bot2011_COI	GB27	1	BB80310_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80310	Sample	BB80310	targeted	Absent	Sample	Upin_Bot2011_COI	GB27	COI	45	BB80310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB27	CTTTAATTACAGCGTTTTTATTGTTGT	151.25757
BB80311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83146	WGS84	BB80311	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80311_Mgig_Bot2012_COI	GB26	1	BB80311_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80311	Sample	BB80311	targeted	Absent	Sample	Mgig_Bot2012_COI	GB26	COI	45	BB80311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB26	TCTTATTCGTTGGAGACTTTATAACCCT	151.2596
BB80312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83228	WGS84	BB80312	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80312_Cmae_Bot2011_COI	GB24	1	BB80312_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80312	Sample	BB80312	targeted	Absent	Sample	Cmae_Bot2011_COI	GB24	COI	45	BB80312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB24	ATGAACAGTCTATCCTCCTTTAG	151.26328
BB80312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83228	WGS84	BB80312	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80312_Msal_Bot2012_COI	GB24	1	BB80312_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80312	Sample	BB80312	targeted	Absent	Sample	Msal_Bot2012_COI	GB24	COI	45	BB80312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB24	GYTAGTTCCRATGATGTTAGCTG	151.26328
BB80314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83452	WGS84	BB80314	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80314_Aamu_Bax2006_COI	GB22	1	BB80314_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80314	Sample	BB80314	targeted	Absent	Sample	Aamu_Bax2006_COI	GB22	COI	45	BB80314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB22	GCACAACCGGGATCTTTACTTC	151.267
BB80314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83452	WGS84	BB80314	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80314_Pcan_Bot2011_IGS	GB22	1	BB80314_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80314	Sample	BB80314	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB22	IGS	45	BB80314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB22	CGTAATCCTCAGTACTGGCTA	151.267
BB80314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83452	WGS84	BB80314	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80314_Sspa_Oph2007_COI	GB22	1	BB80314_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80314	Sample	BB80314	targeted	Absent	Sample	Sspa_Oph2007_COI	GB22	COI	45	BB80314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB22	GAAGGCCTTCCGATGATC	151.267
BB80314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83452	WGS84	BB80314	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80314_Upin_Bot2011_COI	GB22	1	BB80314_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80314	Sample	BB80314	targeted	Absent	Sample	Upin_Bot2011_COI	GB22	COI	45	BB80314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB22	CTTTAATTACAGCGTTTTTATTGTTGT	151.267
BB80315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83503	WGS84	BB80315	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80315_Mgig_Bot2012_COI	GB18	1	BB80315_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80315	Sample	BB80315	targeted	Absent	Sample	Mgig_Bot2012_COI	GB18	COI	45	BB80315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB18	TCTTATTCGTTGGAGACTTTATAACCCT	151.27135
BB80316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85172	WGS84	BB80316	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80316_Cmae_Bot2011_COI	GB13	1	BB80316_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80316	Sample	BB80316	targeted	Absent	Sample	Cmae_Bot2011_COI	GB13	COI	45	BB80316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB13	ATGAACAGTCTATCCTCCTTTAG	151.30872
BB80316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85172	WGS84	BB80316	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80316_Msal_Bot2012_COI	GB13	1	BB80316_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80316	Sample	BB80316	targeted	Absent	Sample	Msal_Bot2012_COI	GB13	COI	45	BB80316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB13	GYTAGTTCCRATGATGTTAGCTG	151.30872
BB80318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85238	WGS84	BB80318	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80318_Aamu_Bax2006_COI	GB8	1	BB80318_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80318	Sample	BB80318	targeted	Absent	Sample	Aamu_Bax2006_COI	GB8	COI	45	BB80318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB8	GCACAACCGGGATCTTTACTTC	151.31641
BB80318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85238	WGS84	BB80318	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80318_Pcan_Bot2011_IGS	GB8	1	BB80318_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80318	Sample	BB80318	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB8	IGS	45	BB80318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB8	CGTAATCCTCAGTACTGGCTA	151.31641
BB80318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85238	WGS84	BB80318	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80318_Sspa_Oph2007_COI	GB8	1	BB80318_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80318	Sample	BB80318	targeted	Absent	Sample	Sspa_Oph2007_COI	GB8	COI	45	BB80318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB8	GAAGGCCTTCCGATGATC	151.31641
BB80318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85238	WGS84	BB80318	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80318_Upin_Bot2011_COI	GB8	1	BB80318_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80318	Sample	BB80318	targeted	Absent	Sample	Upin_Bot2011_COI	GB8	COI	45	BB80318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB8	CTTTAATTACAGCGTTTTTATTGTTGT	151.31641
BB80323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8559	WGS84	BB80323	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80323_Cmae_Bot2011_COI	GB2	1	BB80323_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80323	Sample	BB80323	targeted	Absent	Sample	Cmae_Bot2011_COI	GB2	COI	45	BB80323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB2	ATGAACAGTCTATCCTCCTTTAG	151.32319
BB80323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8559	WGS84	BB80323	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80323_Msal_Bot2012_COI	GB2	1	BB80323_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80323	Sample	BB80323	targeted	Absent	Sample	Msal_Bot2012_COI	GB2	COI	45	BB80323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB2	GYTAGTTCCRATGATGTTAGCTG	151.32319
BB80477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42858	WGS84	BB80477	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80477_Cmae_Bot2011_COI	BB45	1	BB80477_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80477	Sample	BB80477	targeted	Absent	Sample	Cmae_Bot2011_COI	BB45	COI	45	BB80477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB45	ATGAACAGTCTATCCTCCTTTAG	153.12175
BB80477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42858	WGS84	BB80477	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80477_Msal_Bot2012_COI	BB45	1	BB80477_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80477	Sample	BB80477	targeted	Absent	Sample	Msal_Bot2012_COI	BB45	COI	45	BB80477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB45	GYTAGTTCCRATGATGTTAGCTG	153.12175
BB80479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4006	WGS84	BB80479	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80479_Aamu_Bax2006_COI	BB25	1	BB80479_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80479	Sample	BB80479	targeted	Absent	Sample	Aamu_Bax2006_COI	BB25	COI	45	BB80479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB25	GCACAACCGGGATCTTTACTTC	153.16275
BB80479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4006	WGS84	BB80479	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80479_Pcan_Bot2011_IGS	BB25	1	BB80479_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80479	Sample	BB80479	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB25	IGS	45	BB80479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB25	CGTAATCCTCAGTACTGGCTA	153.16275
BB80479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4006	WGS84	BB80479	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80479_Sspa_Oph2007_COI	BB25	1	BB80479_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80479	Sample	BB80479	targeted	Absent	Sample	Sspa_Oph2007_COI	BB25	COI	45	BB80479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB25	GAAGGCCTTCCGATGATC	153.16275
BB80479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4006	WGS84	BB80479	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80479_Upin_Bot2011_COI	BB25	1	BB80479_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80479	Sample	BB80479	targeted	Absent	Sample	Upin_Bot2011_COI	BB25	COI	45	BB80479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB25	CTTTAATTACAGCGTTTTTATTGTTGT	153.16275
BB80480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44671	WGS84	BB80480	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80480_Cmae_Bot2011_COI	BB40	1	BB80480_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80480	Sample	BB80480	targeted	Absent	Sample	Cmae_Bot2011_COI	BB40	COI	45	BB80480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB40	ATGAACAGTCTATCCTCCTTTAG	153.07953
BB80480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44671	WGS84	BB80480	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80480_Msal_Bot2012_COI	BB40	1	BB80480_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80480	Sample	BB80480	targeted	Absent	Sample	Msal_Bot2012_COI	BB40	COI	45	BB80480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB40	GYTAGTTCCRATGATGTTAGCTG	153.07953
BB80482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37816	WGS84	BB80482	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80482_Aamu_Bax2006_COI	BB15	1	BB80482_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80482	Sample	BB80482	targeted	Absent	Sample	Aamu_Bax2006_COI	BB15	COI	45	BB80482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB15	GCACAACCGGGATCTTTACTTC	153.16523
BB80482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37816	WGS84	BB80482	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80482_Pcan_Bot2011_IGS	BB15	1	BB80482_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80482	Sample	BB80482	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB15	IGS	45	BB80482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB15	CGTAATCCTCAGTACTGGCTA	153.16523
BB80482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37816	WGS84	BB80482	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80482_Sspa_Oph2007_COI	BB15	1	BB80482_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80482	Sample	BB80482	targeted	Absent	Sample	Sspa_Oph2007_COI	BB15	COI	45	BB80482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB15	GAAGGCCTTCCGATGATC	153.16523
BB80482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37816	WGS84	BB80482	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80482_Upin_Bot2011_COI	BB15	1	BB80482_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80482	Sample	BB80482	targeted	Absent	Sample	Upin_Bot2011_COI	BB15	COI	45	BB80482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB15	CTTTAATTACAGCGTTTTTATTGTTGT	153.16523
BB80483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41377	WGS84	BB80483	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80483_Mgig_Bot2012_COI	BB57	1	BB80483_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80483	Sample	BB80483	targeted	Absent	Sample	Mgig_Bot2012_COI	BB57	COI	45	BB80483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB57	TCTTATTCGTTGGAGACTTTATAACCCT	153.14357
BB80484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41854	WGS84	BB80484	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80484_Cmae_Bot2011_COI	BB56	1	BB80484_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80484	Sample	BB80484	targeted	Absent	Sample	Cmae_Bot2011_COI	BB56	COI	45	BB80484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB56	ATGAACAGTCTATCCTCCTTTAG	153.13951
BB80484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41854	WGS84	BB80484	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80484_Msal_Bot2012_COI	BB56	1	BB80484_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80484	Sample	BB80484	targeted	Absent	Sample	Msal_Bot2012_COI	BB56	COI	45	BB80484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB56	GYTAGTTCCRATGATGTTAGCTG	153.13951
BB80486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42478	WGS84	BB80486	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80486_Aamu_Bax2006_COI	BB53	1	BB80486_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80486	Sample	BB80486	targeted	Absent	Sample	Aamu_Bax2006_COI	BB53	COI	45	BB80486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB53	GCACAACCGGGATCTTTACTTC	153.12951
BB80486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42478	WGS84	BB80486	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80486_Pcan_Bot2011_IGS	BB53	1	BB80486_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80486	Sample	BB80486	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB53	IGS	45	BB80486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB53	CGTAATCCTCAGTACTGGCTA	153.12951
BB80486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42478	WGS84	BB80486	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80486_Sspa_Oph2007_COI	BB53	1	BB80486_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80486	Sample	BB80486	targeted	Absent	Sample	Sspa_Oph2007_COI	BB53	COI	45	BB80486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB53	GAAGGCCTTCCGATGATC	153.12951
BB80486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42478	WGS84	BB80486	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80486_Upin_Bot2011_COI	BB53	1	BB80486_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80486	Sample	BB80486	targeted	Absent	Sample	Upin_Bot2011_COI	BB53	COI	45	BB80486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB53	CTTTAATTACAGCGTTTTTATTGTTGT	153.12951
BB80487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42763	WGS84	BB80487	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80487_Mgig_Bot2012_COI	BB52	1	BB80487_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80487	Sample	BB80487	targeted	Absent	Sample	Mgig_Bot2012_COI	BB52	COI	45	BB80487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB52	TCTTATTCGTTGGAGACTTTATAACCCT	153.12369
BB80488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4305	WGS84	BB80488	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80488_Cmae_Bot2011_COI	BB47	1	BB80488_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80488	Sample	BB80488	targeted	Absent	Sample	Cmae_Bot2011_COI	BB47	COI	45	BB80488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB47	ATGAACAGTCTATCCTCCTTTAG	153.12348
BB80488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4305	WGS84	BB80488	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80488_Msal_Bot2012_COI	BB47	1	BB80488_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80488	Sample	BB80488	targeted	Absent	Sample	Msal_Bot2012_COI	BB47	COI	45	BB80488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB47	GYTAGTTCCRATGATGTTAGCTG	153.12348
BB80490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40702	WGS84	BB80490	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80490_Mgig_Bot2012_COI	BB62	1	BB80490_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80490	Sample	BB80490	targeted	Absent	Sample	Mgig_Bot2012_COI	BB62	COI	45	BB80490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB62	TCTTATTCGTTGGAGACTTTATAACCCT	153.15063
BB80491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4011	WGS84	BB80491	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80491_Cmae_Bot2011_COI	BB58	1	BB80491_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80491	Sample	BB80491	targeted	Absent	Sample	Cmae_Bot2011_COI	BB58	COI	45	BB80491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB58	ATGAACAGTCTATCCTCCTTTAG	153.15819
BB80491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4011	WGS84	BB80491	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80491_Msal_Bot2012_COI	BB58	1	BB80491_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80491	Sample	BB80491	targeted	Absent	Sample	Msal_Bot2012_COI	BB58	COI	45	BB80491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB58	GYTAGTTCCRATGATGTTAGCTG	153.15819
BB80493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44583	WGS84	BB80493	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80493_Aamu_Bax2006_COI	BB42	1	BB80493_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80493	Sample	BB80493	targeted	Absent	Sample	Aamu_Bax2006_COI	BB42	COI	45	BB80493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB42	GCACAACCGGGATCTTTACTTC	153.08345
BB80493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44583	WGS84	BB80493	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80493_Pcan_Bot2011_IGS	BB42	1	BB80493_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80493	Sample	BB80493	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB42	IGS	45	BB80493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB42	CGTAATCCTCAGTACTGGCTA	153.08345
BB80493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44583	WGS84	BB80493	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80493_Sspa_Oph2007_COI	BB42	1	BB80493_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80493	Sample	BB80493	targeted	Absent	Sample	Sspa_Oph2007_COI	BB42	COI	45	BB80493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB42	GAAGGCCTTCCGATGATC	153.08345
BB80493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44583	WGS84	BB80493	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80493_Upin_Bot2011_COI	BB42	1	BB80493_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80493	Sample	BB80493	targeted	Absent	Sample	Upin_Bot2011_COI	BB42	COI	45	BB80493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB42	CTTTAATTACAGCGTTTTTATTGTTGT	153.08345
BB80494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44631	WGS84	BB80494	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80494_Mgig_Bot2012_COI	BB39	1	BB80494_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80494	Sample	BB80494	targeted	Absent	Sample	Mgig_Bot2012_COI	BB39	COI	45	BB80494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB39	TCTTATTCGTTGGAGACTTTATAACCCT	153.0775
BB80495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44492	WGS84	BB80495	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80495_Cmae_Bot2011_COI	BB37	1	BB80495_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80495	Sample	BB80495	targeted	Absent	Sample	Cmae_Bot2011_COI	BB37	COI	45	BB80495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB37	ATGAACAGTCTATCCTCCTTTAG	153.07984
BB80495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44492	WGS84	BB80495	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80495_Msal_Bot2012_COI	BB37	1	BB80495_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80495	Sample	BB80495	targeted	Absent	Sample	Msal_Bot2012_COI	BB37	COI	45	BB80495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB37	GYTAGTTCCRATGATGTTAGCTG	153.07984
BB80497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44567	WGS84	BB80497	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80497_Aamu_Bax2006_COI	BB34	1	BB80497_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80497	Sample	BB80497	targeted	Absent	Sample	Aamu_Bax2006_COI	BB34	COI	45	BB80497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB34	GCACAACCGGGATCTTTACTTC	153.07495
BB80497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44567	WGS84	BB80497	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80497_Pcan_Bot2011_IGS	BB34	1	BB80497_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80497	Sample	BB80497	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB34	IGS	45	BB80497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB34	CGTAATCCTCAGTACTGGCTA	153.07495
BB80497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44567	WGS84	BB80497	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80497_Sspa_Oph2007_COI	BB34	1	BB80497_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80497	Sample	BB80497	targeted	Absent	Sample	Sspa_Oph2007_COI	BB34	COI	45	BB80497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB34	GAAGGCCTTCCGATGATC	153.07495
BB80497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44567	WGS84	BB80497	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80497_Upin_Bot2011_COI	BB34	1	BB80497_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80497	Sample	BB80497	targeted	Absent	Sample	Upin_Bot2011_COI	BB34	COI	45	BB80497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB34	CTTTAATTACAGCGTTTTTATTGTTGT	153.07495
BB80498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44397	WGS84	BB80498	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80498_Mgig_Bot2012_COI	BB31	1	BB80498_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80498	Sample	BB80498	targeted	Absent	Sample	Mgig_Bot2012_COI	BB31	COI	45	BB80498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB31	TCTTATTCGTTGGAGACTTTATAACCCT	153.07629
BB80499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44256	WGS84	BB80499	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80499_Cmae_Bot2011_COI	BB29	1	BB80499_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80499	Sample	BB80499	targeted	Absent	Sample	Cmae_Bot2011_COI	BB29	COI	45	BB80499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB29	ATGAACAGTCTATCCTCCTTTAG	153.07224
BB80499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44256	WGS84	BB80499	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80499_Msal_Bot2012_COI	BB29	1	BB80499_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80499	Sample	BB80499	targeted	Absent	Sample	Msal_Bot2012_COI	BB29	COI	45	BB80499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB29	GYTAGTTCCRATGATGTTAGCTG	153.07224
BB80501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36806	WGS84	BB80501	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80501_Aamu_Bax2006_COI	BB8	1	BB80501_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80501	Sample	BB80501	targeted	Absent	Sample	Aamu_Bax2006_COI	BB8	COI	45	BB80501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB8	GCACAACCGGGATCTTTACTTC	153.17231
BB80501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36806	WGS84	BB80501	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80501_Pcan_Bot2011_IGS	BB8	1	BB80501_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80501	Sample	BB80501	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB8	IGS	45	BB80501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB8	CGTAATCCTCAGTACTGGCTA	153.17231
BB80501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36806	WGS84	BB80501	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80501_Sspa_Oph2007_COI	BB8	1	BB80501_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80501	Sample	BB80501	targeted	Absent	Sample	Sspa_Oph2007_COI	BB8	COI	45	BB80501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB8	GAAGGCCTTCCGATGATC	153.17231
BB80501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36806	WGS84	BB80501	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80501_Upin_Bot2011_COI	BB8	1	BB80501_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80501	Sample	BB80501	targeted	Absent	Sample	Upin_Bot2011_COI	BB8	COI	45	BB80501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB8	CTTTAATTACAGCGTTTTTATTGTTGT	153.17231
BB80502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36487	WGS84	BB80502	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80502_Mgig_Bot2012_COI	BB6	1	BB80502_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80502	Sample	BB80502	targeted	Absent	Sample	Mgig_Bot2012_COI	BB6	COI	45	BB80502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB6	TCTTATTCGTTGGAGACTTTATAACCCT	153.1748
BB80503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36134	WGS84	BB80503	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80503_Cmae_Bot2011_COI	BB4	1	BB80503_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80503	Sample	BB80503	targeted	Absent	Sample	Cmae_Bot2011_COI	BB4	COI	45	BB80503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB4	ATGAACAGTCTATCCTCCTTTAG	153.17733
BB80503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36134	WGS84	BB80503	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80503_Msal_Bot2012_COI	BB4	1	BB80503_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80503	Sample	BB80503	targeted	Absent	Sample	Msal_Bot2012_COI	BB4	COI	45	BB80503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB4	GYTAGTTCCRATGATGTTAGCTG	153.17733
BB87783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84762	WGS84	BB87783	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87783_Cjap_Sim2018_COI	T11-01	1	BB87783_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87783	Sample	BB87783	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-01	COI	45	BB87783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87521
BB87783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.84762	WGS84	BB87783	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.138	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	27/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87783_Mgig_Bot2012_COI	T11-01	1	BB87783_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87783	Sample	BB87783	targeted	Present	Sample	Mgig_Bot2012_COI	T11-01	COI	45	BB87783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.87521
BB87784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85098	WGS84	BB87784	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87784_Cmae_Bot2011_COI	T11-02	1	BB87784_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87784	Sample	BB87784	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-02	COI	45	BB87784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-02	ATGAACAGTCTATCCTCCTTTAG	147.87402
BB87786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88943	WGS84	BB87786	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87786_Aamu_Bax2006_COI	T11-04	1	BB87786_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87786	Sample	BB87786	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-04	COI	45	BB87786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-04	GCACAACCGGGATCTTTACTTC	147.81458
BB87786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88943	WGS84	BB87786	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87786_Pcan_Bot2011_IGS	T11-04	1	BB87786_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87786	Sample	BB87786	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-04	IGS	45	BB87786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-04	CGTAATCCTCAGTACTGGCTA	147.81458
BB87786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88943	WGS84	BB87786	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87786_Sspa_Oph2007_COI	T11-04	1	BB87786_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87786	Sample	BB87786	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-04	COI	45	BB87786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-04	GAAGGCCTTCCGATGATC	147.81458
BB87786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88943	WGS84	BB87786	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.109	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87786_Upin_Bot2011_COI	T11-04	1	BB87786_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87786	Sample	BB87786	targeted	Present	Sample	Upin_Bot2011_COI	T11-04	COI	45	BB87786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.81458
BB87787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96278	WGS84	BB87787	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87787_Cjap_Sim2018_COI	T11-05	1	BB87787_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87787	Sample	BB87787	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-05	COI	45	BB87787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53883
BB87787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96278	WGS84	BB87787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.224	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	29/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87787_Mgig_Bot2012_COI	T11-05	1	BB87787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87787	Sample	BB87787	targeted	Present	Sample	Mgig_Bot2012_COI	T11-05	COI	45	BB87787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.53883
BB87788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.95998	WGS84	BB87788	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87788_Cmae_Bot2011_COI	T11-06	1	BB87788_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87788	Sample	BB87788	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-06	COI	45	BB87788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-06	ATGAACAGTCTATCCTCCTTTAG	147.52818
BB87790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82515	WGS84	BB87790	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87790_Cjap_Sim2018_COI	T11-08	1	BB87790_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87790	Sample	BB87790	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-08	COI	45	BB87790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3078
BB87790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82515	WGS84	BB87790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.378	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87790_Mgig_Bot2012_COI	T11-08	1	BB87790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87790	Sample	BB87790	targeted	Present	Sample	Mgig_Bot2012_COI	T11-08	COI	45	BB87790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.3078
BB87791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8275	WGS84	BB87791	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87791_Cmae_Bot2011_COI	T11-09	1	BB87791_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87791	Sample	BB87791	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-09	COI	45	BB87791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-09	ATGAACAGTCTATCCTCCTTTAG	147.30215
BB87793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87793	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87793_Aamu_Bax2006_COI	T11-11	1	BB87793_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87793	Sample	BB87793	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-11	COI	45	BB87793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-11	GCACAACCGGGATCTTTACTTC	147.33688
BB87793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87793	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87793_Pcan_Bot2011_IGS	T11-11	1	BB87793_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87793	Sample	BB87793	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-11	IGS	45	BB87793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-11	CGTAATCCTCAGTACTGGCTA	147.33688
BB87793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87793	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87793_Sspa_Oph2007_COI	T11-11	1	BB87793_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87793	Sample	BB87793	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-11	COI	45	BB87793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-11	GAAGGCCTTCCGATGATC	147.33688
BB87793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87793	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87793_Upin_Bot2011_COI	T11-11	1	BB87793_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87793	Sample	BB87793	targeted	Absent	Sample	Upin_Bot2011_COI	T11-11	COI	45	BB87793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33688
BB87794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88265	WGS84	BB87794	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87794_Cjap_Sim2018_COI	T11-13	1	BB87794_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87794	Sample	BB87794	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-13	COI	45	BB87794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33628
BB87794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88265	WGS84	BB87794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87794_Mgig_Bot2012_COI	T11-13	1	BB87794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87794	Sample	BB87794	targeted	Absent	Sample	Mgig_Bot2012_COI	T11-13	COI	45	BB87794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.33628
BB87795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88453	WGS84	BB87795	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87795_Cmae_Bot2011_COI	T11-12	1	BB87795_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87795	Sample	BB87795	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-12	COI	45	BB87795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-12	ATGAACAGTCTATCCTCCTTTAG	147.33611
BB87797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87836	WGS84	BB87797	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87797_Aamu_Bax2006_COI	T11-15	1	BB87797_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87797	Sample	BB87797	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-15	COI	45	BB87797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-15	GCACAACCGGGATCTTTACTTC	147.34177
BB87797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87836	WGS84	BB87797	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87797_Pcan_Bot2011_IGS	T11-15	1	BB87797_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87797	Sample	BB87797	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-15	IGS	45	BB87797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-15	CGTAATCCTCAGTACTGGCTA	147.34177
BB87797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87836	WGS84	BB87797	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87797_Sspa_Oph2007_COI	T11-15	1	BB87797_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87797	Sample	BB87797	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-15	COI	45	BB87797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-15	GAAGGCCTTCCGATGATC	147.34177
BB87797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87836	WGS84	BB87797	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87797_Upin_Bot2011_COI	T11-15	1	BB87797_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87797	Sample	BB87797	targeted	Absent	Sample	Upin_Bot2011_COI	T11-15	COI	45	BB87797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.34177
BB87798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87305	WGS84	BB87798	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87798_Cjap_Sim2018_COI	T11-16	1	BB87798_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87798	Sample	BB87798	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-16	COI	45	BB87798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3363
BB87798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87305	WGS84	BB87798	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.711	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87798_Mgig_Bot2012_COI	T11-16	1	BB87798_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87798	Sample	BB87798	targeted	Present	Sample	Mgig_Bot2012_COI	T11-16	COI	45	BB87798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.3363
BB87799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84645	WGS84	BB87799	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.105	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87799_Cmae_Bot2011_COI	S7-02	1	BB87799_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87799	Sample	BB87799	targeted	Present	Sample	Cmae_Bot2011_COI	S7-02	COI	45	BB87799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-02	ATGAACAGTCTATCCTCCTTTAG	138.49705
BB87803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83649	WGS84	BB87803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.037	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87803_Cmae_Bot2011_COI	S7-06	1	BB87803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87803	Sample	BB87803	targeted	Present	Sample	Cmae_Bot2011_COI	S7-06	COI	45	BB87803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-06	ATGAACAGTCTATCCTCCTTTAG	138.50852
BB87805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87805_Aamu_Bax2006_COI	S7-08	1	BB87805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87805	Sample	BB87805	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-08	COI	45	BB87805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-08	GCACAACCGGGATCTTTACTTC	138.51031
BB87805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87805	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87805_Pcan_Bot2011_IGS	S7-08	1	BB87805_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87805	Sample	BB87805	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-08	IGS	45	BB87805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-08	CGTAATCCTCAGTACTGGCTA	138.51031
BB87805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.08	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87805_Sspa_Oph2007_COI	S7-08	1	BB87805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87805	Sample	BB87805	targeted	Present	Sample	Sspa_Oph2007_COI	S7-08	COI	45	BB87805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-08	GAAGGCCTTCCGATGATC	138.51031
BB87805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87805_Upin_Bot2011_COI	S7-08	1	BB87805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87805	Sample	BB87805	targeted	Absent	Sample	Upin_Bot2011_COI	S7-08	COI	45	BB87805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51031
BB87809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78326	WGS84	BB87809	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87809_Aamu_Bax2006_COI	S7-12	1	BB87809_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87809	Sample	BB87809	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-12	COI	45	BB87809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-12	GCACAACCGGGATCTTTACTTC	138.51601
BB87809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78326	WGS84	BB87809	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87809_Pcan_Bot2011_IGS	S7-12	1	BB87809_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87809	Sample	BB87809	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-12	IGS	45	BB87809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-12	CGTAATCCTCAGTACTGGCTA	138.51601
BB87809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78326	WGS84	BB87809	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87809_Sspa_Oph2007_COI	S7-12	1	BB87809_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87809	Sample	BB87809	targeted	Absent	Sample	Sspa_Oph2007_COI	S7-12	COI	45	BB87809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-12	GAAGGCCTTCCGATGATC	138.51601
BB87809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78326	WGS84	BB87809	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87809_Upin_Bot2011_COI	S7-12	1	BB87809_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87809	Sample	BB87809	targeted	Absent	Sample	Upin_Bot2011_COI	S7-12	COI	45	BB87809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.51601
BB87810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78245	WGS84	BB87810	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.146	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87810_Cmae_Bot2011_COI	S7-13	1	BB87810_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87810	Sample	BB87810	targeted	Present	Sample	Cmae_Bot2011_COI	S7-13	COI	45	BB87810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-13	ATGAACAGTCTATCCTCCTTTAG	138.51632
BB87813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77223	WGS84	BB87813	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87813_Cjap_Sim2018_COI	S7-16	1	BB87813_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87813	Sample	BB87813	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-16	COI	45	BB87813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48596
BB87813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77223	WGS84	BB87813	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.978	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87813_Mgig_Bot2012_COI	S7-16	1	BB87813_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87813	Sample	BB87813	targeted	Present	Sample	Mgig_Bot2012_COI	S7-16	COI	45	BB87813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.48596
BB87814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77378	WGS84	BB87814	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.284	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87814_Cmae_Bot2011_COI	S7-17	1	BB87814_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87814	Sample	BB87814	targeted	Present	Sample	Cmae_Bot2011_COI	S7-17	COI	45	BB87814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-17	ATGAACAGTCTATCCTCCTTTAG	138.4857
BB87818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80928	WGS84	BB87818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.16	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87818_Cmae_Bot2011_COI	S7-21	1	BB87818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87818	Sample	BB87818	targeted	Present	Sample	Cmae_Bot2011_COI	S7-21	COI	45	BB87818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-21	ATGAACAGTCTATCCTCCTTTAG	138.51791
BB87823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84719	WGS84	BB87823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87823_Aamu_Bax2006_COI	S7-01	1	BB87823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87823	Sample	BB87823	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-01	COI	45	BB87823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-01	GCACAACCGGGATCTTTACTTC	138.4975
BB87823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84719	WGS84	BB87823	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87823_Pcan_Bot2011_IGS	S7-01	1	BB87823_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87823	Sample	BB87823	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-01	IGS	45	BB87823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-01	CGTAATCCTCAGTACTGGCTA	138.4975
BB87823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84719	WGS84	BB87823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.726	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87823_Sspa_Oph2007_COI	S7-01	1	BB87823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87823	Sample	BB87823	targeted	Present	Sample	Sspa_Oph2007_COI	S7-01	COI	45	BB87823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-01	GAAGGCCTTCCGATGATC	138.4975
BB87823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84719	WGS84	BB87823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87823_Upin_Bot2011_COI	S7-01	1	BB87823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87823	Sample	BB87823	targeted	Absent	Sample	Upin_Bot2011_COI	S7-01	COI	45	BB87823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.4975
BB87824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84768	WGS84	BB87824	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87824_Cjap_Sim2018_COI	T12-01	1	BB87824_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87824	Sample	BB87824	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-01	COI	45	BB87824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.8754
BB87824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.84768	WGS84	BB87824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	28/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87824_Mgig_Bot2012_COI	T12-01	1	BB87824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87824	Sample	BB87824	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-01	COI	45	BB87824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.8754
BB87825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85088	WGS84	BB87825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87825_Cmae_Bot2011_COI	T12-02	1	BB87825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87825	Sample	BB87825	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-02	COI	45	BB87825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-02	ATGAACAGTCTATCCTCCTTTAG	147.87377
BB87827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88956	WGS84	BB87827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87827_Aamu_Bax2006_COI	T12-04	1	BB87827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87827	Sample	BB87827	targeted	Absent	Sample	Aamu_Bax2006_COI	T12-04	COI	45	BB87827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-04	GCACAACCGGGATCTTTACTTC	147.81287
BB87827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88956	WGS84	BB87827	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87827_Pcan_Bot2011_IGS	T12-04	1	BB87827_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87827	Sample	BB87827	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-04	IGS	45	BB87827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-04	CGTAATCCTCAGTACTGGCTA	147.81287
BB87827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88956	WGS84	BB87827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87827_Sspa_Oph2007_COI	T12-04	1	BB87827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87827	Sample	BB87827	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-04	COI	45	BB87827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-04	GAAGGCCTTCCGATGATC	147.81287
BB87827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88956	WGS84	BB87827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.107	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87827_Upin_Bot2011_COI	T12-04	1	BB87827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87827	Sample	BB87827	targeted	Present	Sample	Upin_Bot2011_COI	T12-04	COI	45	BB87827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.81287
BB87828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88174	WGS84	BB87828	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87828_Cjap_Sim2018_COI	T12-05	1	BB87828_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87828	Sample	BB87828	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-05	COI	45	BB87828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34223
BB87828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88174	WGS84	BB87828	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87828_Mgig_Bot2012_COI	T12-05	1	BB87828_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87828	Sample	BB87828	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-05	COI	45	BB87828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.34223
BB87829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87783	WGS84	BB87829	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87829_Cmae_Bot2011_COI	T12-06	1	BB87829_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87829	Sample	BB87829	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-06	COI	45	BB87829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-06	ATGAACAGTCTATCCTCCTTTAG	147.34193
BB87830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87348	WGS84	BB87830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.726	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87830_Aamu_Bax2006_COI	T12-07	1	BB87830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87830	Sample	BB87830	targeted	Present	Sample	Aamu_Bax2006_COI	T12-07	COI	45	BB87830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-07	GCACAACCGGGATCTTTACTTC	147.33661
BB87830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87348	WGS84	BB87830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87830_Pcan_Bot2011_IGS	T12-07	1	BB87830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87830	Sample	BB87830	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-07	IGS	45	BB87830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-07	CGTAATCCTCAGTACTGGCTA	147.33661
BB87830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87348	WGS84	BB87830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87830_Sspa_Oph2007_COI	T12-07	1	BB87830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87830	Sample	BB87830	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-07	COI	45	BB87830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-07	GAAGGCCTTCCGATGATC	147.33661
BB87830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87348	WGS84	BB87830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87830_Upin_Bot2011_COI	T12-07	1	BB87830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87830	Sample	BB87830	targeted	Absent	Sample	Upin_Bot2011_COI	T12-07	COI	45	BB87830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.33661
BB87831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82866	WGS84	BB87831	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87831_Cjap_Sim2018_COI	T12-08	1	BB87831_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87831	Sample	BB87831	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-08	COI	45	BB87831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30169
BB87831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82866	WGS84	BB87831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87831_Mgig_Bot2012_COI	T12-08	1	BB87831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87831	Sample	BB87831	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-08	COI	45	BB87831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.30169
BB87832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82729	WGS84	BB87832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87832_Cmae_Bot2011_COI	T12-09	1	BB87832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87832	Sample	BB87832	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-09	COI	45	BB87832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-09	ATGAACAGTCTATCCTCCTTTAG	147.30174
BB87834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88553	WGS84	BB87834	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.465	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87834_Aamu_Bax2006_COI	T12-11	1	BB87834_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87834	Sample	BB87834	targeted	Present	Sample	Aamu_Bax2006_COI	T12-11	COI	45	BB87834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-11	GCACAACCGGGATCTTTACTTC	147.33569
BB87834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88553	WGS84	BB87834	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87834_Pcan_Bot2011_IGS	T12-11	1	BB87834_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87834	Sample	BB87834	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-11	IGS	45	BB87834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-11	CGTAATCCTCAGTACTGGCTA	147.33569
BB87834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88553	WGS84	BB87834	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87834_Sspa_Oph2007_COI	T12-11	1	BB87834_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87834	Sample	BB87834	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-11	COI	45	BB87834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-11	GAAGGCCTTCCGATGATC	147.33569
BB87834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88553	WGS84	BB87834	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87834_Upin_Bot2011_COI	T12-11	1	BB87834_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87834	Sample	BB87834	targeted	Absent	Sample	Upin_Bot2011_COI	T12-11	COI	45	BB87834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33569
BB87835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88464	WGS84	BB87835	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87835_Cjap_Sim2018_COI	T12-12	1	BB87835_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87835	Sample	BB87835	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-12	COI	45	BB87835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33707
BB87835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88464	WGS84	BB87835	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87835_Mgig_Bot2012_COI	T12-12	1	BB87835_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87835	Sample	BB87835	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-12	COI	45	BB87835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.33707
BB87836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88287	WGS84	BB87836	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87836_Cmae_Bot2011_COI	T12-13	1	BB87836_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87836	Sample	BB87836	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-13	COI	45	BB87836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-13	ATGAACAGTCTATCCTCCTTTAG	147.33643
BB87839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83645	WGS84	BB87839	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87839_Cjap_Sim2018_COI	S8-24	1	BB87839_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87839	Sample	BB87839	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-24	COI	45	BB87839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-24	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50846
BB87839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83645	WGS84	BB87839	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.671	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87839_Mgig_Bot2012_COI	S8-24	1	BB87839_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87839	Sample	BB87839	targeted	Present	Sample	Mgig_Bot2012_COI	S8-24	COI	45	BB87839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-24	TCTTATTCGTTGGAGACTTTATAACCCT	138.50846
BB87841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96032	WGS84	BB87841	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.231	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87841_Aamu_Bax2006_COI	T13-02	1	BB87841_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87841	Sample	BB87841	targeted	Present	Sample	Aamu_Bax2006_COI	T13-02	COI	45	BB87841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-02	GCACAACCGGGATCTTTACTTC	147.53078
BB87841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96032	WGS84	BB87841	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87841_Pcan_Bot2011_IGS	T13-02	1	BB87841_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87841	Sample	BB87841	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-02	IGS	45	BB87841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-02	CGTAATCCTCAGTACTGGCTA	147.53078
BB87841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96032	WGS84	BB87841	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87841_Sspa_Oph2007_COI	T13-02	1	BB87841_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87841	Sample	BB87841	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-02	COI	45	BB87841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-02	GAAGGCCTTCCGATGATC	147.53078
BB87841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96032	WGS84	BB87841	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87841_Upin_Bot2011_COI	T13-02	1	BB87841_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87841	Sample	BB87841	targeted	Absent	Sample	Upin_Bot2011_COI	T13-02	COI	45	BB87841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.53078
BB87842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96236	WGS84	BB87842	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87842_Cjap_Sim2018_COI	T13-03	1	BB87842_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87842	Sample	BB87842	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-03	COI	45	BB87842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51954
BB87842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96236	WGS84	BB87842	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87842_Mgig_Bot2012_COI	T13-03	1	BB87842_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87842	Sample	BB87842	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-03	COI	45	BB87842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.51954
BB87843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84846	WGS84	BB87843	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87843_Cmae_Bot2011_COI	T13-04	1	BB87843_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87843	Sample	BB87843	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-04	COI	45	BB87843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-04	ATGAACAGTCTATCCTCCTTTAG	147.87427
BB87845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85552	WGS84	BB87845	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87845_Aamu_Bax2006_COI	T13-06	1	BB87845_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87845	Sample	BB87845	targeted	Absent	Sample	Aamu_Bax2006_COI	T13-06	COI	45	BB87845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-06	GCACAACCGGGATCTTTACTTC	147.87256
BB87845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85552	WGS84	BB87845	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87845_Pcan_Bot2011_IGS	T13-06	1	BB87845_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87845	Sample	BB87845	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-06	IGS	45	BB87845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-06	CGTAATCCTCAGTACTGGCTA	147.87256
BB87845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85552	WGS84	BB87845	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87845_Sspa_Oph2007_COI	T13-06	1	BB87845_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87845	Sample	BB87845	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-06	COI	45	BB87845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-06	GAAGGCCTTCCGATGATC	147.87256
BB87845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85552	WGS84	BB87845	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87845_Upin_Bot2011_COI	T13-06	1	BB87845_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87845	Sample	BB87845	targeted	Absent	Sample	Upin_Bot2011_COI	T13-06	COI	45	BB87845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.87256
BB87846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88938	WGS84	BB87846	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87846_Cjap_Sim2018_COI	T13-07	1	BB87846_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87846	Sample	BB87846	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-07	COI	45	BB87846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81479
BB87846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88938	WGS84	BB87846	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87846_Mgig_Bot2012_COI	T13-07	1	BB87846_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87846	Sample	BB87846	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-07	COI	45	BB87846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.81479
BB87847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8289	WGS84	BB87847	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87847_Cmae_Bot2011_COI	T13-08	1	BB87847_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87847	Sample	BB87847	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-08	COI	45	BB87847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-08	ATGAACAGTCTATCCTCCTTTAG	147.30228
BB87849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82561	WGS84	BB87849	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87849_Aamu_Bax2006_COI	T13-10	1	BB87849_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87849	Sample	BB87849	targeted	Absent	Sample	Aamu_Bax2006_COI	T13-10	COI	45	BB87849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-10	GCACAACCGGGATCTTTACTTC	147.30381
BB87849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82561	WGS84	BB87849	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87849_Pcan_Bot2011_IGS	T13-10	1	BB87849_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87849	Sample	BB87849	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-10	IGS	45	BB87849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-10	CGTAATCCTCAGTACTGGCTA	147.30381
BB87849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82561	WGS84	BB87849	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87849_Sspa_Oph2007_COI	T13-10	1	BB87849_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87849	Sample	BB87849	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-10	COI	45	BB87849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-10	GAAGGCCTTCCGATGATC	147.30381
BB87849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82561	WGS84	BB87849	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87849_Upin_Bot2011_COI	T13-10	1	BB87849_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87849	Sample	BB87849	targeted	Absent	Sample	Upin_Bot2011_COI	T13-10	COI	45	BB87849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.30381
BB87850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87321	WGS84	BB87850	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.539	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87850_Cmae_Bot2011_COI	T13-11	1	BB87850_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87850	Sample	BB87850	targeted	Present	Sample	Cmae_Bot2011_COI	T13-11	COI	45	BB87850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-11	ATGAACAGTCTATCCTCCTTTAG	147.33664
BB87852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87988	WGS84	BB87852	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.975	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87852_Aamu_Bax2006_COI	T13-13	1	BB87852_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87852	Sample	BB87852	targeted	Present	Sample	Aamu_Bax2006_COI	T13-13	COI	45	BB87852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-13	GCACAACCGGGATCTTTACTTC	147.3422
BB87852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87988	WGS84	BB87852	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87852_Pcan_Bot2011_IGS	T13-13	1	BB87852_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87852	Sample	BB87852	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-13	IGS	45	BB87852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-13	CGTAATCCTCAGTACTGGCTA	147.3422
BB87852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87988	WGS84	BB87852	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87852_Sspa_Oph2007_COI	T13-13	1	BB87852_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87852	Sample	BB87852	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-13	COI	45	BB87852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-13	GAAGGCCTTCCGATGATC	147.3422
BB87852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87988	WGS84	BB87852	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87852_Upin_Bot2011_COI	T13-13	1	BB87852_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87852	Sample	BB87852	targeted	Absent	Sample	Upin_Bot2011_COI	T13-13	COI	45	BB87852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.3422
BB87853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.86907	WGS84	BB87853	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87853_Cjap_Sim2018_COI	T13-14	1	BB87853_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87853	Sample	BB87853	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-14	COI	45	BB87853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33633
BB87853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.86907	WGS84	BB87853	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87853_Mgig_Bot2012_COI	T13-14	1	BB87853_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87853	Sample	BB87853	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-14	COI	45	BB87853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.33633
BB87854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88452	WGS84	BB87854	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87854_Cmae_Bot2011_COI	T13-15	1	BB87854_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87854	Sample	BB87854	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-15	COI	45	BB87854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-15	ATGAACAGTCTATCCTCCTTTAG	147.33658
BB87856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84432	WGS84	BB87856	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87856_Aamu_Bax2006_COI	S8-16	1	BB87856_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87856	Sample	BB87856	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-16	COI	45	BB87856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-16	GCACAACCGGGATCTTTACTTC	138.49778
BB87856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84432	WGS84	BB87856	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87856_Pcan_Bot2011_IGS	S8-16	1	BB87856_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87856	Sample	BB87856	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-16	IGS	45	BB87856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-16	CGTAATCCTCAGTACTGGCTA	138.49778
BB87856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84432	WGS84	BB87856	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.074	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87856_Sspa_Oph2007_COI	S8-16	1	BB87856_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87856	Sample	BB87856	targeted	Present	Sample	Sspa_Oph2007_COI	S8-16	COI	45	BB87856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-16	GAAGGCCTTCCGATGATC	138.49778
BB87856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84432	WGS84	BB87856	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87856_Upin_Bot2011_COI	S8-16	1	BB87856_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87856	Sample	BB87856	targeted	Absent	Sample	Upin_Bot2011_COI	S8-16	COI	45	BB87856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.49778
BB87860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77362	WGS84	BB87860	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87860_Cjap_Sim2018_COI	S8-12	1	BB87860_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87860	Sample	BB87860	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-12	COI	45	BB87860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48487
BB87860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77362	WGS84	BB87860	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87860_Mgig_Bot2012_COI	S8-12	1	BB87860_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87860	Sample	BB87860	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-12	COI	45	BB87860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.48487
BB87861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77196	WGS84	BB87861	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87861_Cmae_Bot2011_COI	S8-11	1	BB87861_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87861	Sample	BB87861	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-11	COI	45	BB87861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-11	ATGAACAGTCTATCCTCCTTTAG	138.48602
BB87863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83652	WGS84	BB87863	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87863_Aamu_Bax2006_COI	S8-21	1	BB87863_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87863	Sample	BB87863	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-21	COI	45	BB87863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-21	GCACAACCGGGATCTTTACTTC	138.50853
BB87863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83652	WGS84	BB87863	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87863_Pcan_Bot2011_IGS	S8-21	1	BB87863_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87863	Sample	BB87863	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-21	IGS	45	BB87863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-21	CGTAATCCTCAGTACTGGCTA	138.50853
BB87863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83652	WGS84	BB87863	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.5	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87863_Sspa_Oph2007_COI	S8-21	1	BB87863_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87863	Sample	BB87863	targeted	Present	Sample	Sspa_Oph2007_COI	S8-21	COI	45	BB87863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-21	GAAGGCCTTCCGATGATC	138.50853
BB87863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83652	WGS84	BB87863	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87863_Upin_Bot2011_COI	S8-21	1	BB87863_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87863	Sample	BB87863	targeted	Absent	Sample	Upin_Bot2011_COI	S8-21	COI	45	BB87863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-21	CTTTAATTACAGCGTTTTTATTGTTGT	138.50853
BB87864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78706	WGS84	BB87864	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87864_Cjap_Sim2018_COI	S8-08	1	BB87864_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87864	Sample	BB87864	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-08	COI	45	BB87864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51353
BB87864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78706	WGS84	BB87864	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.051	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87864_Mgig_Bot2012_COI	S8-08	1	BB87864_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87864	Sample	BB87864	targeted	Present	Sample	Mgig_Bot2012_COI	S8-08	COI	45	BB87864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51353
BB87865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78822	WGS84	BB87865	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87865_Cmae_Bot2011_COI	S8-07	1	BB87865_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87865	Sample	BB87865	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-07	COI	45	BB87865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-07	ATGAACAGTCTATCCTCCTTTAG	138.51314
BB87867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84712	WGS84	BB87867	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87867_Aamu_Bax2006_COI	S8-18	1	BB87867_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87867	Sample	BB87867	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-18	COI	45	BB87867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-18	GCACAACCGGGATCTTTACTTC	138.4976
BB87867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84712	WGS84	BB87867	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87867_Pcan_Bot2011_IGS	S8-18	1	BB87867_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87867	Sample	BB87867	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-18	IGS	45	BB87867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-18	CGTAATCCTCAGTACTGGCTA	138.4976
BB87867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84712	WGS84	BB87867	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.097	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87867_Sspa_Oph2007_COI	S8-18	1	BB87867_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87867	Sample	BB87867	targeted	Present	Sample	Sspa_Oph2007_COI	S8-18	COI	45	BB87867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-18	GAAGGCCTTCCGATGATC	138.4976
BB87867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84712	WGS84	BB87867	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87867_Upin_Bot2011_COI	S8-18	1	BB87867_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87867	Sample	BB87867	targeted	Absent	Sample	Upin_Bot2011_COI	S8-18	COI	45	BB87867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.4976
BB87868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87868	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87868_Cjap_Sim2018_COI	S8-17	1	BB87868_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87868	Sample	BB87868	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-17	COI	45	BB87868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49707
BB87868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84557	WGS84	BB87868	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.809	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87868_Mgig_Bot2012_COI	S8-17	1	BB87868_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87868	Sample	BB87868	targeted	Present	Sample	Mgig_Bot2012_COI	S8-17	COI	45	BB87868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.49707
BB87869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80964	WGS84	BB87869	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.049	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87869_Cmae_Bot2011_COI	S8-03	1	BB87869_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87869	Sample	BB87869	targeted	Present	Sample	Cmae_Bot2011_COI	S8-03	COI	45	BB87869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-03	ATGAACAGTCTATCCTCCTTTAG	138.52021
BB87870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80894	WGS84	BB87870	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87870_Aamu_Bax2006_COI	S8-02	1	BB87870_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87870	Sample	BB87870	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-02	COI	45	BB87870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-02	GCACAACCGGGATCTTTACTTC	138.51889
BB87870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80894	WGS84	BB87870	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87870_Pcan_Bot2011_IGS	S8-02	1	BB87870_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87870	Sample	BB87870	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-02	IGS	45	BB87870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-02	CGTAATCCTCAGTACTGGCTA	138.51889
BB87870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80894	WGS84	BB87870	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.213	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87870_Sspa_Oph2007_COI	S8-02	1	BB87870_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87870	Sample	BB87870	targeted	Present	Sample	Sspa_Oph2007_COI	S8-02	COI	45	BB87870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-02	GAAGGCCTTCCGATGATC	138.51889
BB87870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80894	WGS84	BB87870	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87870_Upin_Bot2011_COI	S8-02	1	BB87870_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87870	Sample	BB87870	targeted	Absent	Sample	Upin_Bot2011_COI	S8-02	COI	45	BB87870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.51889
BB87871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81008	WGS84	BB87871	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87871_Cjap_Sim2018_COI	S8-01	1	BB87871_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87871	Sample	BB87871	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-01	COI	45	BB87871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51996
BB87871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.81008	WGS84	BB87871	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87871_Mgig_Bot2012_COI	S8-01	1	BB87871_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87871	Sample	BB87871	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-01	COI	45	BB87871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.51996
BB87872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81034	WGS84	BB87872	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87872_Cmae_Bot2011_COI	S6-25	1	BB87872_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87872	Sample	BB87872	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-25	COI	45	BB87872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-25	ATGAACAGTCTATCCTCCTTTAG	138.52098
BB87875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8111	WGS84	BB87875	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87875_Cjap_Sim2018_COI	S6-22	1	BB87875_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87875	Sample	BB87875	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-22	COI	45	BB87875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-22	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52346
BB87875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.8111	WGS84	BB87875	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.071	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87875_Mgig_Bot2012_COI	S6-22	1	BB87875_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87875	Sample	BB87875	targeted	Present	Sample	Mgig_Bot2012_COI	S6-22	COI	45	BB87875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-22	TCTTATTCGTTGGAGACTTTATAACCCT	138.52346
BB87876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81155	WGS84	BB87876	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	41.511	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87876_Cmae_Bot2011_COI	S6-21	1	BB87876_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87876	Sample	BB87876	targeted	Present	Sample	Cmae_Bot2011_COI	S6-21	COI	45	BB87876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-21	ATGAACAGTCTATCCTCCTTTAG	138.52436
BB87879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77353	WGS84	BB87879	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87879_Cjap_Sim2018_COI	S6-18	1	BB87879_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87879	Sample	BB87879	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-18	COI	45	BB87879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48586
BB87879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77353	WGS84	BB87879	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87879_Mgig_Bot2012_COI	S6-18	1	BB87879_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87879	Sample	BB87879	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-18	COI	45	BB87879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.48586
BB87881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77307	WGS84	BB87881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87881_Aamu_Bax2006_COI	S6-16	1	BB87881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87881	Sample	BB87881	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-16	COI	45	BB87881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-16	GCACAACCGGGATCTTTACTTC	138.48439
BB87881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77307	WGS84	BB87881	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87881_Pcan_Bot2011_IGS	S6-16	1	BB87881_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87881	Sample	BB87881	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-16	IGS	45	BB87881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-16	CGTAATCCTCAGTACTGGCTA	138.48439
BB87881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77307	WGS84	BB87881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87881_Sspa_Oph2007_COI	S6-16	1	BB87881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87881	Sample	BB87881	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-16	COI	45	BB87881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-16	GAAGGCCTTCCGATGATC	138.48439
BB87881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77307	WGS84	BB87881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87881_Upin_Bot2011_COI	S6-16	1	BB87881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87881	Sample	BB87881	targeted	Absent	Sample	Upin_Bot2011_COI	S6-16	COI	45	BB87881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.48439
BB87885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78633	WGS84	BB87885	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87885_Aamu_Bax2006_COI	S6-12	1	BB87885_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87885	Sample	BB87885	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-12	COI	45	BB87885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-12	GCACAACCGGGATCTTTACTTC	138.51551
BB87885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78633	WGS84	BB87885	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87885_Pcan_Bot2011_IGS	S6-12	1	BB87885_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87885	Sample	BB87885	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-12	IGS	45	BB87885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-12	CGTAATCCTCAGTACTGGCTA	138.51551
BB87885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78633	WGS84	BB87885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87885_Sspa_Oph2007_COI	S6-12	1	BB87885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87885	Sample	BB87885	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-12	COI	45	BB87885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-12	GAAGGCCTTCCGATGATC	138.51551
BB87885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78633	WGS84	BB87885	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87885_Upin_Bot2011_COI	S6-12	1	BB87885_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87885	Sample	BB87885	targeted	Absent	Sample	Upin_Bot2011_COI	S6-12	COI	45	BB87885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.51551
BB87886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78716	WGS84	BB87886	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87886_Cjap_Sim2018_COI	S6-11	1	BB87886_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87886	Sample	BB87886	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-11	COI	45	BB87886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51506
BB87886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78716	WGS84	BB87886	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87886_Mgig_Bot2012_COI	S6-11	1	BB87886_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87886	Sample	BB87886	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-11	COI	45	BB87886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51506
BB87889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83736	WGS84	BB87889	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87889_Aamu_Bax2006_COI	S6-08	1	BB87889_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87889	Sample	BB87889	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-08	COI	45	BB87889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-08	GCACAACCGGGATCTTTACTTC	138.51038
BB87889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83736	WGS84	BB87889	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87889_Pcan_Bot2011_IGS	S6-08	1	BB87889_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87889	Sample	BB87889	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-08	IGS	45	BB87889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-08	CGTAATCCTCAGTACTGGCTA	138.51038
BB87889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83736	WGS84	BB87889	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.86	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87889_Sspa_Oph2007_COI	S6-08	1	BB87889_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87889	Sample	BB87889	targeted	Present	Sample	Sspa_Oph2007_COI	S6-08	COI	45	BB87889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-08	GAAGGCCTTCCGATGATC	138.51038
BB87889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83736	WGS84	BB87889	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87889_Upin_Bot2011_COI	S6-08	1	BB87889_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87889	Sample	BB87889	targeted	Absent	Sample	Upin_Bot2011_COI	S6-08	COI	45	BB87889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51038
BB87890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83688	WGS84	BB87890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87890_Cmae_Bot2011_COI	S6-07	1	BB87890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87890	Sample	BB87890	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-07	COI	45	BB87890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-07	ATGAACAGTCTATCCTCCTTTAG	138.50928
BB87894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84522	WGS84	BB87894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87894_Cmae_Bot2011_COI	S6-03	1	BB87894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87894	Sample	BB87894	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-03	COI	45	BB87894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-03	ATGAACAGTCTATCCTCCTTTAG	138.49694
BB87896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87896	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87896_Aamu_Bax2006_COI	S6-01	1	BB87896_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87896	Sample	BB87896	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-01	COI	45	BB87896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-01	GCACAACCGGGATCTTTACTTC	138.49739
BB87896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87896	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87896_Pcan_Bot2011_IGS	S6-01	1	BB87896_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87896	Sample	BB87896	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-01	IGS	45	BB87896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-01	CGTAATCCTCAGTACTGGCTA	138.49739
BB87896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87896_Sspa_Oph2007_COI	S6-01	1	BB87896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87896	Sample	BB87896	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-01	COI	45	BB87896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-01	GAAGGCCTTCCGATGATC	138.49739
BB87896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87896	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87896_Upin_Bot2011_COI	S6-01	1	BB87896_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87896	Sample	BB87896	targeted	Absent	Sample	Upin_Bot2011_COI	S6-01	COI	45	BB87896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.49739
BB87897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85592	WGS84	BB87897	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87897_Cjap_Sim2018_COI	T10-16	1	BB87897_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87897	Sample	BB87897	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-16	COI	45	BB87897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87241
BB87897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85592	WGS84	BB87897	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.642	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	29/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87897_Mgig_Bot2012_COI	T10-16	1	BB87897_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87897	Sample	BB87897	targeted	Present	Sample	Mgig_Bot2012_COI	T10-16	COI	45	BB87897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.87241
BB87898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85085	WGS84	BB87898	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.218	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87898_Cmae_Bot2011_COI	T10-15	1	BB87898_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87898	Sample	BB87898	targeted	Present	Sample	Cmae_Bot2011_COI	T10-15	COI	45	BB87898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-15	ATGAACAGTCTATCCTCCTTTAG	147.87391
BB87900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88961	WGS84	BB87900	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87900_Aamu_Bax2006_COI	T10-13	1	BB87900_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87900	Sample	BB87900	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-13	COI	45	BB87900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-13	GCACAACCGGGATCTTTACTTC	147.81247
BB87900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88961	WGS84	BB87900	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87900_Pcan_Bot2011_IGS	T10-13	1	BB87900_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87900	Sample	BB87900	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-13	IGS	45	BB87900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-13	CGTAATCCTCAGTACTGGCTA	147.81247
BB87900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88961	WGS84	BB87900	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87900_Sspa_Oph2007_COI	T10-13	1	BB87900_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87900	Sample	BB87900	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-13	COI	45	BB87900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-13	GAAGGCCTTCCGATGATC	147.81247
BB87900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88961	WGS84	BB87900	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87900_Upin_Bot2011_COI	T10-13	1	BB87900_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87900	Sample	BB87900	targeted	Absent	Sample	Upin_Bot2011_COI	T10-13	COI	45	BB87900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.81247
BB87901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88273	WGS84	BB87901	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87901_Cjap_Sim2018_COI	T10-12	1	BB87901_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87901	Sample	BB87901	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-12	COI	45	BB87901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33619
BB87901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88273	WGS84	BB87901	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.598	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87901_Mgig_Bot2012_COI	T10-12	1	BB87901_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87901	Sample	BB87901	targeted	Present	Sample	Mgig_Bot2012_COI	T10-12	COI	45	BB87901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.33619
BB87902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88419	WGS84	BB87902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87902_Cmae_Bot2011_COI	T10-11	1	BB87902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87902	Sample	BB87902	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-11	COI	45	BB87902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-11	ATGAACAGTCTATCCTCCTTTAG	147.33632
BB87904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87881	WGS84	BB87904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87904_Aamu_Bax2006_COI	T10-09	1	BB87904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87904	Sample	BB87904	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-09	COI	45	BB87904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-09	GCACAACCGGGATCTTTACTTC	147.34167
BB87904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87881	WGS84	BB87904	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87904_Pcan_Bot2011_IGS	T10-09	1	BB87904_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87904	Sample	BB87904	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-09	IGS	45	BB87904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-09	CGTAATCCTCAGTACTGGCTA	147.34167
BB87904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87881	WGS84	BB87904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87904_Sspa_Oph2007_COI	T10-09	1	BB87904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87904	Sample	BB87904	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-09	COI	45	BB87904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-09	GAAGGCCTTCCGATGATC	147.34167
BB87904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87881	WGS84	BB87904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87904_Upin_Bot2011_COI	T10-09	1	BB87904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87904	Sample	BB87904	targeted	Absent	Sample	Upin_Bot2011_COI	T10-09	COI	45	BB87904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.34167
BB87905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88078	WGS84	BB87905	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87905_Cjap_Sim2018_COI	T10-08	1	BB87905_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87905	Sample	BB87905	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-08	COI	45	BB87905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34172
BB87905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88078	WGS84	BB87905	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.69	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87905_Mgig_Bot2012_COI	T10-08	1	BB87905_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87905	Sample	BB87905	targeted	Present	Sample	Mgig_Bot2012_COI	T10-08	COI	45	BB87905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.34172
BB87906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8729	WGS84	BB87906	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87906_Cmae_Bot2011_COI	T10-07	1	BB87906_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87906	Sample	BB87906	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-07	COI	45	BB87906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-07	ATGAACAGTCTATCCTCCTTTAG	147.3363
BB87908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82753	WGS84	BB87908	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87908_Aamu_Bax2006_COI	T10-05	1	BB87908_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87908	Sample	BB87908	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-05	COI	45	BB87908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-05	GCACAACCGGGATCTTTACTTC	147.30173
BB87908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82753	WGS84	BB87908	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87908_Pcan_Bot2011_IGS	T10-05	1	BB87908_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87908	Sample	BB87908	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-05	IGS	45	BB87908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-05	CGTAATCCTCAGTACTGGCTA	147.30173
BB87908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82753	WGS84	BB87908	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87908_Sspa_Oph2007_COI	T10-05	1	BB87908_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87908	Sample	BB87908	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-05	COI	45	BB87908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-05	GAAGGCCTTCCGATGATC	147.30173
BB87908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82753	WGS84	BB87908	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87908_Upin_Bot2011_COI	T10-05	1	BB87908_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87908	Sample	BB87908	targeted	Absent	Sample	Upin_Bot2011_COI	T10-05	COI	45	BB87908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.30173
BB87909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82538	WGS84	BB87909	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87909_Cjap_Sim2018_COI	T10-04	1	BB87909_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87909	Sample	BB87909	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-04	COI	45	BB87909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30734
BB87909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82538	WGS84	BB87909	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.251	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87909_Mgig_Bot2012_COI	T10-04	1	BB87909_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87909	Sample	BB87909	targeted	Present	Sample	Mgig_Bot2012_COI	T10-04	COI	45	BB87909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.30734
BB87911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96019	WGS84	BB87911	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.041	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87911_Aamu_Bax2006_COI	T10-02	1	BB87911_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87911	Sample	BB87911	targeted	Present	Sample	Aamu_Bax2006_COI	T10-02	COI	45	BB87911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-02	GCACAACCGGGATCTTTACTTC	147.53113
BB87911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96019	WGS84	BB87911	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87911_Pcan_Bot2011_IGS	T10-02	1	BB87911_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87911	Sample	BB87911	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-02	IGS	45	BB87911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-02	CGTAATCCTCAGTACTGGCTA	147.53113
BB87911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96019	WGS84	BB87911	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87911_Sspa_Oph2007_COI	T10-02	1	BB87911_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87911	Sample	BB87911	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-02	COI	45	BB87911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-02	GAAGGCCTTCCGATGATC	147.53113
BB87911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96019	WGS84	BB87911	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87911_Upin_Bot2011_COI	T10-02	1	BB87911_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87911	Sample	BB87911	targeted	Absent	Sample	Upin_Bot2011_COI	T10-02	COI	45	BB87911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.53113
BB87912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96295	WGS84	BB87912	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87912_Cjap_Sim2018_COI	T10-01	1	BB87912_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87912	Sample	BB87912	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-01	COI	45	BB87912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53907
BB87912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96295	WGS84	BB87912	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.857	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87912_Mgig_Bot2012_COI	T10-01	1	BB87912_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87912	Sample	BB87912	targeted	Present	Sample	Mgig_Bot2012_COI	T10-01	COI	45	BB87912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.53907
BB87915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81003	WGS84	BB87915	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87915_Aamu_Bax2006_COI	S5-22	1	BB87915_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87915	Sample	BB87915	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-22	COI	45	BB87915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-22	GCACAACCGGGATCTTTACTTC	138.52013
BB87915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81003	WGS84	BB87915	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87915_Pcan_Bot2011_IGS	S5-22	1	BB87915_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87915	Sample	BB87915	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-22	IGS	45	BB87915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-22	CGTAATCCTCAGTACTGGCTA	138.52013
BB87915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81003	WGS84	BB87915	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.712	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87915_Sspa_Oph2007_COI	S5-22	1	BB87915_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87915	Sample	BB87915	targeted	Present	Sample	Sspa_Oph2007_COI	S5-22	COI	45	BB87915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-22	GAAGGCCTTCCGATGATC	138.52013
BB87915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81003	WGS84	BB87915	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87915_Upin_Bot2011_COI	S5-22	1	BB87915_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87915	Sample	BB87915	targeted	Absent	Sample	Upin_Bot2011_COI	S5-22	COI	45	BB87915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-22	CTTTAATTACAGCGTTTTTATTGTTGT	138.52013
BB87916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81005	WGS84	BB87916	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87916_Cjap_Sim2018_COI	S5-21	1	BB87916_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87916	Sample	BB87916	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-21	COI	45	BB87916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-21	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51897
BB87916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.81005	WGS84	BB87916	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.339	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87916_Mgig_Bot2012_COI	S5-21	1	BB87916_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87916	Sample	BB87916	targeted	Present	Sample	Mgig_Bot2012_COI	S5-21	COI	45	BB87916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-21	TCTTATTCGTTGGAGACTTTATAACCCT	138.51897
BB87919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77349	WGS84	BB87919	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87919_Aamu_Bax2006_COI	S5-18	1	BB87919_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87919	Sample	BB87919	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-18	COI	45	BB87919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-18	GCACAACCGGGATCTTTACTTC	138.48577
BB87919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77349	WGS84	BB87919	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87919_Pcan_Bot2011_IGS	S5-18	1	BB87919_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87919	Sample	BB87919	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-18	IGS	45	BB87919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-18	CGTAATCCTCAGTACTGGCTA	138.48577
BB87919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77349	WGS84	BB87919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.325	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87919_Sspa_Oph2007_COI	S5-18	1	BB87919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87919	Sample	BB87919	targeted	Present	Sample	Sspa_Oph2007_COI	S5-18	COI	45	BB87919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-18	GAAGGCCTTCCGATGATC	138.48577
BB87919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77349	WGS84	BB87919	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87919_Upin_Bot2011_COI	S5-18	1	BB87919_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87919	Sample	BB87919	targeted	Absent	Sample	Upin_Bot2011_COI	S5-18	COI	45	BB87919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.48577
BB87920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77247	WGS84	BB87920	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87920_Cmae_Bot2011_COI	S5-17	1	BB87920_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87920	Sample	BB87920	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-17	COI	45	BB87920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-17	ATGAACAGTCTATCCTCCTTTAG	138.4855
BB87924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78534	WGS84	BB87924	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87924_Cmae_Bot2011_COI	S5-13	1	BB87924_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87924	Sample	BB87924	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-13	COI	45	BB87924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-13	ATGAACAGTCTATCCTCCTTTAG	138.51572
BB87926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78764	WGS84	BB87926	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87926_Aamu_Bax2006_COI	S5-11	1	BB87926_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87926	Sample	BB87926	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-11	COI	45	BB87926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-11	GCACAACCGGGATCTTTACTTC	138.51513
BB87926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78764	WGS84	BB87926	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87926_Pcan_Bot2011_IGS	S5-11	1	BB87926_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87926	Sample	BB87926	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-11	IGS	45	BB87926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-11	CGTAATCCTCAGTACTGGCTA	138.51513
BB87926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78764	WGS84	BB87926	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.352	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87926_Sspa_Oph2007_COI	S5-11	1	BB87926_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87926	Sample	BB87926	targeted	Present	Sample	Sspa_Oph2007_COI	S5-11	COI	45	BB87926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-11	GAAGGCCTTCCGATGATC	138.51513
BB87926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78764	WGS84	BB87926	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87926_Upin_Bot2011_COI	S5-11	1	BB87926_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87926	Sample	BB87926	targeted	Absent	Sample	Upin_Bot2011_COI	S5-11	COI	45	BB87926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.51513
BB87930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83622	WGS84	BB87930	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87930_Cjap_Sim2018_COI	S5-07	1	BB87930_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87930	Sample	BB87930	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-07	COI	45	BB87930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50894
BB87930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83622	WGS84	BB87930	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87930_Mgig_Bot2012_COI	S5-07	1	BB87930_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87930	Sample	BB87930	targeted	Absent	Sample	Mgig_Bot2012_COI	S5-07	COI	45	BB87930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.50894
BB87931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87931	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87931_Cmae_Bot2011_COI	S5-06	1	BB87931_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87931	Sample	BB87931	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-06	COI	45	BB87931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-06	ATGAACAGTCTATCCTCCTTTAG	138.50759
BB87934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8452	WGS84	BB87934	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87934_Cjap_Sim2018_COI	S5-03	1	BB87934_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87934	Sample	BB87934	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-03	COI	45	BB87934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49715
BB87934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.8452	WGS84	BB87934	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.206	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87934_Mgig_Bot2012_COI	S5-03	1	BB87934_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87934	Sample	BB87934	targeted	Present	Sample	Mgig_Bot2012_COI	S5-03	COI	45	BB87934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.49715
BB87935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84612	WGS84	BB87935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87935_Cmae_Bot2011_COI	S5-02	1	BB87935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87935	Sample	BB87935	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-02	COI	45	BB87935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-02	ATGAACAGTCTATCCTCCTTTAG	138.49706
BB87937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88278	WGS84	BB87937	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87937_Aamu_Bax2006_COI	T9-16	1	BB87937_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87937	Sample	BB87937	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-16	COI	45	BB87937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-16	GCACAACCGGGATCTTTACTTC	147.33629
BB87937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88278	WGS84	BB87937	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87937_Pcan_Bot2011_IGS	T9-16	1	BB87937_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87937	Sample	BB87937	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-16	IGS	45	BB87937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-16	CGTAATCCTCAGTACTGGCTA	147.33629
BB87937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88278	WGS84	BB87937	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87937_Sspa_Oph2007_COI	T9-16	1	BB87937_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87937	Sample	BB87937	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-16	COI	45	BB87937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-16	GAAGGCCTTCCGATGATC	147.33629
BB87937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88278	WGS84	BB87937	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87937_Upin_Bot2011_COI	T9-16	1	BB87937_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87937	Sample	BB87937	targeted	Absent	Sample	Upin_Bot2011_COI	T9-16	COI	45	BB87937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.33629
BB87938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88434	WGS84	BB87938	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87938_Cjap_Sim2018_COI	T9-15	1	BB87938_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87938	Sample	BB87938	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-15	COI	45	BB87938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33624
BB87938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88434	WGS84	BB87938	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.988	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87938_Mgig_Bot2012_COI	T9-15	1	BB87938_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87938	Sample	BB87938	targeted	Present	Sample	Mgig_Bot2012_COI	T9-15	COI	45	BB87938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.33624
BB87939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88567	WGS84	BB87939	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87939_Cmae_Bot2011_COI	T9-14	1	BB87939_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87939	Sample	BB87939	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-14	COI	45	BB87939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-14	ATGAACAGTCTATCCTCCTTTAG	147.33529
BB87940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87919	WGS84	BB87940	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87940_Aamu_Bax2006_COI	T9-13	1	BB87940_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87940	Sample	BB87940	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-13	COI	45	BB87940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-13	GCACAACCGGGATCTTTACTTC	147.3417
BB87940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87919	WGS84	BB87940	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87940_Pcan_Bot2011_IGS	T9-13	1	BB87940_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87940	Sample	BB87940	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-13	IGS	45	BB87940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-13	CGTAATCCTCAGTACTGGCTA	147.3417
BB87940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87919	WGS84	BB87940	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87940_Sspa_Oph2007_COI	T9-13	1	BB87940_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87940	Sample	BB87940	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-13	COI	45	BB87940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-13	GAAGGCCTTCCGATGATC	147.3417
BB87940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87919	WGS84	BB87940	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87940_Upin_Bot2011_COI	T9-13	1	BB87940_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87940	Sample	BB87940	targeted	Absent	Sample	Upin_Bot2011_COI	T9-13	COI	45	BB87940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.3417
BB87941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8813	WGS84	BB87941	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87941_Cjap_Sim2018_COI	T9-12	1	BB87941_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87941	Sample	BB87941	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-12	COI	45	BB87941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3416
BB87941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.8813	WGS84	BB87941	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.154	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87941_Mgig_Bot2012_COI	T9-12	1	BB87941_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87941	Sample	BB87941	targeted	Present	Sample	Mgig_Bot2012_COI	T9-12	COI	45	BB87941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.3416
BB87942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87302	WGS84	BB87942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87942_Cmae_Bot2011_COI	T9-11	1	BB87942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87942	Sample	BB87942	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-11	COI	45	BB87942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-11	ATGAACAGTCTATCCTCCTTTAG	147.33632
BB87944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82698	WGS84	BB87944	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87944_Aamu_Bax2006_COI	T9-09	1	BB87944_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87944	Sample	BB87944	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-09	COI	45	BB87944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-09	GCACAACCGGGATCTTTACTTC	147.30243
BB87944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82698	WGS84	BB87944	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87944_Pcan_Bot2011_IGS	T9-09	1	BB87944_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87944	Sample	BB87944	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-09	IGS	45	BB87944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-09	CGTAATCCTCAGTACTGGCTA	147.30243
BB87944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82698	WGS84	BB87944	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87944_Sspa_Oph2007_COI	T9-09	1	BB87944_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87944	Sample	BB87944	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-09	COI	45	BB87944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-09	GAAGGCCTTCCGATGATC	147.30243
BB87944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82698	WGS84	BB87944	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87944_Upin_Bot2011_COI	T9-09	1	BB87944_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87944	Sample	BB87944	targeted	Absent	Sample	Upin_Bot2011_COI	T9-09	COI	45	BB87944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.30243
BB87945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8292	WGS84	BB87945	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87945_Cjap_Sim2018_COI	T9-08	1	BB87945_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87945	Sample	BB87945	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-08	COI	45	BB87945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30183
BB87945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.8292	WGS84	BB87945	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.541	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87945_Mgig_Bot2012_COI	T9-08	1	BB87945_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87945	Sample	BB87945	targeted	Present	Sample	Mgig_Bot2012_COI	T9-08	COI	45	BB87945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.30183
BB87946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85585	WGS84	BB87946	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87946_Cmae_Bot2011_COI	T9-07	1	BB87946_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87946	Sample	BB87946	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-07	COI	45	BB87946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-07	ATGAACAGTCTATCCTCCTTTAG	147.87239
BB87948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84699	WGS84	BB87948	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87948_Aamu_Bax2006_COI	T9-05	1	BB87948_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87948	Sample	BB87948	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-05	COI	45	BB87948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-05	GCACAACCGGGATCTTTACTTC	147.87517
BB87948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84699	WGS84	BB87948	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87948_Pcan_Bot2011_IGS	T9-05	1	BB87948_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87948	Sample	BB87948	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-05	IGS	45	BB87948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-05	CGTAATCCTCAGTACTGGCTA	147.87517
BB87948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84699	WGS84	BB87948	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87948_Sspa_Oph2007_COI	T9-05	1	BB87948_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87948	Sample	BB87948	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-05	COI	45	BB87948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-05	GAAGGCCTTCCGATGATC	147.87517
BB87948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84699	WGS84	BB87948	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.163	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87948_Upin_Bot2011_COI	T9-05	1	BB87948_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87948	Sample	BB87948	targeted	Present	Sample	Upin_Bot2011_COI	T9-05	COI	45	BB87948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.87517
BB87949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88957	WGS84	BB87949	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87949_Cjap_Sim2018_COI	T9-04	1	BB87949_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87949	Sample	BB87949	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-04	COI	45	BB87949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81295
BB87949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88957	WGS84	BB87949	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.323	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	23/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87949_Mgig_Bot2012_COI	T9-04	1	BB87949_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87949	Sample	BB87949	targeted	Present	Sample	Mgig_Bot2012_COI	T9-04	COI	45	BB87949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.81295
BB87951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96033	WGS84	BB87951	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87951_Aamu_Bax2006_COI	T9-02	1	BB87951_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87951	Sample	BB87951	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-02	COI	45	BB87951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-02	GCACAACCGGGATCTTTACTTC	147.53072
BB87951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96033	WGS84	BB87951	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87951_Pcan_Bot2011_IGS	T9-02	1	BB87951_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87951	Sample	BB87951	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-02	IGS	45	BB87951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-02	CGTAATCCTCAGTACTGGCTA	147.53072
BB87951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96033	WGS84	BB87951	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87951_Sspa_Oph2007_COI	T9-02	1	BB87951_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87951	Sample	BB87951	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-02	COI	45	BB87951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-02	GAAGGCCTTCCGATGATC	147.53072
BB87951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96033	WGS84	BB87951	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87951_Upin_Bot2011_COI	T9-02	1	BB87951_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87951	Sample	BB87951	targeted	Absent	Sample	Upin_Bot2011_COI	T9-02	COI	45	BB87951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.53072
BB87952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96322	WGS84	BB87952	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87952_Cjap_Sim2018_COI	T9-01	1	BB87952_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87952	Sample	BB87952	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-01	COI	45	BB87952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.5383
BB87952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96322	WGS84	BB87952	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.449	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	22/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87952_Mgig_Bot2012_COI	T9-01	1	BB87952_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87952	Sample	BB87952	targeted	Present	Sample	Mgig_Bot2012_COI	T9-01	COI	45	BB87952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.5383
BB87955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80982	WGS84	BB87955	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87955_Aamu_Bax2006_COI	S4-18	1	BB87955_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87955	Sample	BB87955	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-18	COI	45	BB87955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-18	GCACAACCGGGATCTTTACTTC	138.51977
BB87955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80982	WGS84	BB87955	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87955_Pcan_Bot2011_IGS	S4-18	1	BB87955_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87955	Sample	BB87955	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-18	IGS	45	BB87955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-18	CGTAATCCTCAGTACTGGCTA	138.51977
BB87955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80982	WGS84	BB87955	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87955_Sspa_Oph2007_COI	S4-18	1	BB87955_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87955	Sample	BB87955	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-18	COI	45	BB87955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-18	GAAGGCCTTCCGATGATC	138.51977
BB87955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80982	WGS84	BB87955	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87955_Upin_Bot2011_COI	S4-18	1	BB87955_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87955	Sample	BB87955	targeted	Absent	Sample	Upin_Bot2011_COI	S4-18	COI	45	BB87955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.51977
BB87956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80946	WGS84	BB87956	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87956_Cjap_Sim2018_COI	S4-17	1	BB87956_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87956	Sample	BB87956	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-17	COI	45	BB87956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51847
BB87956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.80946	WGS84	BB87956	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.515	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87956_Mgig_Bot2012_COI	S4-17	1	BB87956_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87956	Sample	BB87956	targeted	Present	Sample	Mgig_Bot2012_COI	S4-17	COI	45	BB87956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.51847
BB87957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80913	WGS84	BB87957	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87957_Cmae_Bot2011_COI	S4-16	1	BB87957_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87957	Sample	BB87957	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-16	COI	45	BB87957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-16	ATGAACAGTCTATCCTCCTTTAG	138.51747
BB93412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85169	WGS84	BB93412	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93412_Mgig_Bot2012_COI	GD95	1	BB93412_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93412	Sample	BB93412	targeted	Absent	Sample	Mgig_Bot2012_COI	GD95	COI	45	BB93412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD95	TCTTATTCGTTGGAGACTTTATAACCCT	151.31021
BB93413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85318	WGS84	BB93413	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93413_Cmae_Bot2011_COI	GD90	1	BB93413_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93413	Sample	BB93413	targeted	Absent	Sample	Cmae_Bot2011_COI	GD90	COI	45	BB93413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD90	ATGAACAGTCTATCCTCCTTTAG	151.31829
BB93413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85318	WGS84	BB93413	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93413_Msal_Bot2012_COI	GD90	1	BB93413_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93413	Sample	BB93413	targeted	Absent	Sample	Msal_Bot2012_COI	GD90	COI	45	BB93413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD90	GYTAGTTCCRATGATGTTAGCTG	151.31829
BB93415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78606	WGS84	BB93415	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93415_Aamu_Bax2006_COI	GD70	1	BB93415_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93415	Sample	BB93415	targeted	Absent	Sample	Aamu_Bax2006_COI	GD70	COI	45	BB93415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD70	GCACAACCGGGATCTTTACTTC	151.17164
BB93415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78606	WGS84	BB93415	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93415_Pcan_Bot2011_IGS	GD70	1	BB93415_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93415	Sample	BB93415	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD70	IGS	45	BB93415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD70	CGTAATCCTCAGTACTGGCTA	151.17164
BB93415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78606	WGS84	BB93415	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93415_Sspa_Oph2007_COI	GD70	1	BB93415_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93415	Sample	BB93415	targeted	Absent	Sample	Sspa_Oph2007_COI	GD70	COI	45	BB93415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD70	GAAGGCCTTCCGATGATC	151.17164
BB93415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78606	WGS84	BB93415	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93415_Upin_Bot2011_COI	GD70	1	BB93415_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93415	Sample	BB93415	targeted	Absent	Sample	Upin_Bot2011_COI	GD70	COI	45	BB93415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD70	CTTTAATTACAGCGTTTTTATTGTTGT	151.17164
BB93416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78758	WGS84	BB93416	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93416_Mgig_Bot2012_COI	GD65	1	BB93416_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93416	Sample	BB93416	targeted	Absent	Sample	Mgig_Bot2012_COI	GD65	COI	45	BB93416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD65	TCTTATTCGTTGGAGACTTTATAACCCT	151.17433
BB93417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79059	WGS84	BB93417	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93417_Cmae_Bot2011_COI	GD60	1	BB93417_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93417	Sample	BB93417	targeted	Absent	Sample	Cmae_Bot2011_COI	GD60	COI	45	BB93417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD60	ATGAACAGTCTATCCTCCTTTAG	151.17623
BB93417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79059	WGS84	BB93417	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93417_Msal_Bot2012_COI	GD60	1	BB93417_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93417	Sample	BB93417	targeted	Absent	Sample	Msal_Bot2012_COI	GD60	COI	45	BB93417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD60	GYTAGTTCCRATGATGTTAGCTG	151.17623
BB93419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81656	WGS84	BB93419	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93419_Aamu_Bax2006_COI	GD107	1	BB93419_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93419	Sample	BB93419	targeted	Absent	Sample	Aamu_Bax2006_COI	GD107	COI	45	BB93419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD107	GCACAACCGGGATCTTTACTTC	151.24038
BB93419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81656	WGS84	BB93419	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93419_Pcan_Bot2011_IGS	GD107	1	BB93419_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93419	Sample	BB93419	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD107	IGS	45	BB93419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD107	CGTAATCCTCAGTACTGGCTA	151.24038
BB93419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81656	WGS84	BB93419	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93419_Sspa_Oph2007_COI	GD107	1	BB93419_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93419	Sample	BB93419	targeted	Absent	Sample	Sspa_Oph2007_COI	GD107	COI	45	BB93419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD107	GAAGGCCTTCCGATGATC	151.24038
BB93419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81656	WGS84	BB93419	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93419_Upin_Bot2011_COI	GD107	1	BB93419_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93419	Sample	BB93419	targeted	Absent	Sample	Upin_Bot2011_COI	GD107	COI	45	BB93419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD107	CTTTAATTACAGCGTTTTTATTGTTGT	151.24038
BB93420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82257	WGS84	BB93420	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93420_Cmae_Bot2011_COI	GD111	1	BB93420_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93420	Sample	BB93420	targeted	Absent	Sample	Cmae_Bot2011_COI	GD111	COI	45	BB93420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD111	ATGAACAGTCTATCCTCCTTTAG	151.24626
BB93420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82257	WGS84	BB93420	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93420_Msal_Bot2012_COI	GD111	1	BB93420_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93420	Sample	BB93420	targeted	Absent	Sample	Msal_Bot2012_COI	GD111	COI	45	BB93420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD111	GYTAGTTCCRATGATGTTAGCTG	151.24626
BB93422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83171	WGS84	BB93422	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93422_Aamu_Bax2006_COI	GD36	1	BB93422_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93422	Sample	BB93422	targeted	Absent	Sample	Aamu_Bax2006_COI	GD36	COI	45	BB93422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD36	GCACAACCGGGATCTTTACTTC	151.25897
BB93422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83171	WGS84	BB93422	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93422_Pcan_Bot2011_IGS	GD36	1	BB93422_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93422	Sample	BB93422	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD36	IGS	45	BB93422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD36	CGTAATCCTCAGTACTGGCTA	151.25897
BB93422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83171	WGS84	BB93422	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93422_Sspa_Oph2007_COI	GD36	1	BB93422_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93422	Sample	BB93422	targeted	Absent	Sample	Sspa_Oph2007_COI	GD36	COI	45	BB93422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD36	GAAGGCCTTCCGATGATC	151.25897
BB93422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83171	WGS84	BB93422	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93422_Upin_Bot2011_COI	GD36	1	BB93422_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93422	Sample	BB93422	targeted	Absent	Sample	Upin_Bot2011_COI	GD36	COI	45	BB93422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD36	CTTTAATTACAGCGTTTTTATTGTTGT	151.25897
BB93423	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83198	WGS84	BB93423	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93423_Mgig_Bot2012_COI	GD37	1	BB93423_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93423	Sample	BB93423	targeted	Absent	Sample	Mgig_Bot2012_COI	GD37	COI	45	BB93423	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD37	TCTTATTCGTTGGAGACTTTATAACCCT	151.26071
BB93424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8344	WGS84	BB93424	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93424_Cmae_Bot2011_COI	GD39	1	BB93424_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93424	Sample	BB93424	targeted	Absent	Sample	Cmae_Bot2011_COI	GD39	COI	45	BB93424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD39	ATGAACAGTCTATCCTCCTTTAG	151.26551
BB93424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8344	WGS84	BB93424	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93424_Msal_Bot2012_COI	GD39	1	BB93424_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93424	Sample	BB93424	targeted	Absent	Sample	Msal_Bot2012_COI	GD39	COI	45	BB93424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD39	GYTAGTTCCRATGATGTTAGCTG	151.26551
BB93426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8062	WGS84	BB93426	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93426_Aamu_Bax2006_COI	GD43	1	BB93426_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93426	Sample	BB93426	targeted	Absent	Sample	Aamu_Bax2006_COI	GD43	COI	45	BB93426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD43	GCACAACCGGGATCTTTACTTC	151.22027
BB93426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8062	WGS84	BB93426	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93426_Pcan_Bot2011_IGS	GD43	1	BB93426_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93426	Sample	BB93426	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD43	IGS	45	BB93426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD43	CGTAATCCTCAGTACTGGCTA	151.22027
BB93426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8062	WGS84	BB93426	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93426_Sspa_Oph2007_COI	GD43	1	BB93426_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93426	Sample	BB93426	targeted	Absent	Sample	Sspa_Oph2007_COI	GD43	COI	45	BB93426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD43	GAAGGCCTTCCGATGATC	151.22027
BB93426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8062	WGS84	BB93426	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93426_Upin_Bot2011_COI	GD43	1	BB93426_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93426	Sample	BB93426	targeted	Absent	Sample	Upin_Bot2011_COI	GD43	COI	45	BB93426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD43	CTTTAATTACAGCGTTTTTATTGTTGT	151.22027
BB93427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83562	WGS84	BB93427	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93427_Mgig_Bot2012_COI	GD101	1	BB93427_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93427	Sample	BB93427	targeted	Absent	Sample	Mgig_Bot2012_COI	GD101	COI	45	BB93427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD101	TCTTATTCGTTGGAGACTTTATAACCCT	151.27317
BB93428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83454	WGS84	BB93428	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93428_Cmae_Bot2011_COI	GD103	1	BB93428_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93428	Sample	BB93428	targeted	Absent	Sample	Cmae_Bot2011_COI	GD103	COI	45	BB93428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD103	ATGAACAGTCTATCCTCCTTTAG	151.26959
BB93428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83454	WGS84	BB93428	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93428_Msal_Bot2012_COI	GD103	1	BB93428_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93428	Sample	BB93428	targeted	Absent	Sample	Msal_Bot2012_COI	GD103	COI	45	BB93428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD103	GYTAGTTCCRATGATGTTAGCTG	151.26959
BB93430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80758	WGS84	BB93430	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93430_Mgig_Bot2012_COI	GD48	1	BB93430_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93430	Sample	BB93430	targeted	Absent	Sample	Mgig_Bot2012_COI	GD48	COI	45	BB93430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD48	TCTTATTCGTTGGAGACTTTATAACCCT	151.21655
BB93431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80543	WGS84	BB93431	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93431_Cmae_Bot2011_COI	GD52	1	BB93431_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93431	Sample	BB93431	targeted	Absent	Sample	Cmae_Bot2011_COI	GD52	COI	45	BB93431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD52	ATGAACAGTCTATCCTCCTTTAG	151.21816
BB93431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80543	WGS84	BB93431	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93431_Msal_Bot2012_COI	GD52	1	BB93431_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93431	Sample	BB93431	targeted	Absent	Sample	Msal_Bot2012_COI	GD52	COI	45	BB93431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD52	GYTAGTTCCRATGATGTTAGCTG	151.21816
BB93433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79121	WGS84	BB93433	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93433_Aamu_Bax2006_COI	GD56	1	BB93433_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93433	Sample	BB93433	targeted	Absent	Sample	Aamu_Bax2006_COI	GD56	COI	45	BB93433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD56	GCACAACCGGGATCTTTACTTC	151.18219
BB93433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79121	WGS84	BB93433	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93433_Pcan_Bot2011_IGS	GD56	1	BB93433_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93433	Sample	BB93433	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD56	IGS	45	BB93433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD56	CGTAATCCTCAGTACTGGCTA	151.18219
BB93433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79121	WGS84	BB93433	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93433_Sspa_Oph2007_COI	GD56	1	BB93433_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93433	Sample	BB93433	targeted	Absent	Sample	Sspa_Oph2007_COI	GD56	COI	45	BB93433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD56	GAAGGCCTTCCGATGATC	151.18219
BB93433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79121	WGS84	BB93433	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93433_Upin_Bot2011_COI	GD56	1	BB93433_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93433	Sample	BB93433	targeted	Absent	Sample	Upin_Bot2011_COI	GD56	COI	45	BB93433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD56	CTTTAATTACAGCGTTTTTATTGTTGT	151.18219
BB93434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78964	WGS84	BB93434	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93434_Mgig_Bot2012_COI	GD58	1	BB93434_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93434	Sample	BB93434	targeted	Absent	Sample	Mgig_Bot2012_COI	GD58	COI	45	BB93434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD58	TCTTATTCGTTGGAGACTTTATAACCCT	151.1796
BB93435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78916	WGS84	BB93435	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93435_Cmae_Bot2011_COI	GD61	1	BB93435_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93435	Sample	BB93435	targeted	Absent	Sample	Cmae_Bot2011_COI	GD61	COI	45	BB93435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD61	ATGAACAGTCTATCCTCCTTTAG	151.17678
BB93435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78916	WGS84	BB93435	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93435_Msal_Bot2012_COI	GD61	1	BB93435_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93435	Sample	BB93435	targeted	Absent	Sample	Msal_Bot2012_COI	GD61	COI	45	BB93435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD61	GYTAGTTCCRATGATGTTAGCTG	151.17678
BB93437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78794	WGS84	BB93437	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93437_Aamu_Bax2006_COI	GD66	1	BB93437_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93437	Sample	BB93437	targeted	Absent	Sample	Aamu_Bax2006_COI	GD66	COI	45	BB93437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD66	GCACAACCGGGATCTTTACTTC	151.17475
BB93437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78794	WGS84	BB93437	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93437_Pcan_Bot2011_IGS	GD66	1	BB93437_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93437	Sample	BB93437	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD66	IGS	45	BB93437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD66	CGTAATCCTCAGTACTGGCTA	151.17475
BB93437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78794	WGS84	BB93437	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93437_Sspa_Oph2007_COI	GD66	1	BB93437_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93437	Sample	BB93437	targeted	Absent	Sample	Sspa_Oph2007_COI	GD66	COI	45	BB93437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD66	GAAGGCCTTCCGATGATC	151.17475
BB93437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78794	WGS84	BB93437	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93437_Upin_Bot2011_COI	GD66	1	BB93437_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93437	Sample	BB93437	targeted	Absent	Sample	Upin_Bot2011_COI	GD66	COI	45	BB93437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD66	CTTTAATTACAGCGTTTTTATTGTTGT	151.17475
BB93438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78494	WGS84	BB93438	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93438_Mgig_Bot2012_COI	GD68	1	BB93438_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93438	Sample	BB93438	targeted	Absent	Sample	Mgig_Bot2012_COI	GD68	COI	45	BB93438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD68	TCTTATTCGTTGGAGACTTTATAACCCT	151.17338
BB93439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78618	WGS84	BB93439	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93439_Cmae_Bot2011_COI	GD69	1	BB93439_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93439	Sample	BB93439	targeted	Absent	Sample	Cmae_Bot2011_COI	GD69	COI	45	BB93439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD69	ATGAACAGTCTATCCTCCTTTAG	151.17238
BB93439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78618	WGS84	BB93439	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93439_Msal_Bot2012_COI	GD69	1	BB93439_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93439	Sample	BB93439	targeted	Absent	Sample	Msal_Bot2012_COI	GD69	COI	45	BB93439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD69	GYTAGTTCCRATGATGTTAGCTG	151.17238
BB93440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7776	WGS84	BB93440	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93440_Aamu_Bax2006_COI	GD72	1	BB93440_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93440	Sample	BB93440	targeted	Absent	Sample	Aamu_Bax2006_COI	GD72	COI	45	BB93440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD72	GCACAACCGGGATCTTTACTTC	151.19514
BB93440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7776	WGS84	BB93440	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93440_Pcan_Bot2011_IGS	GD72	1	BB93440_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93440	Sample	BB93440	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD72	IGS	45	BB93440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD72	CGTAATCCTCAGTACTGGCTA	151.19514
BB93440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7776	WGS84	BB93440	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93440_Sspa_Oph2007_COI	GD72	1	BB93440_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93440	Sample	BB93440	targeted	Absent	Sample	Sspa_Oph2007_COI	GD72	COI	45	BB93440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD72	GAAGGCCTTCCGATGATC	151.19514
BB93440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7776	WGS84	BB93440	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93440_Upin_Bot2011_COI	GD72	1	BB93440_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93440	Sample	BB93440	targeted	Absent	Sample	Upin_Bot2011_COI	GD72	COI	45	BB93440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD72	CTTTAATTACAGCGTTTTTATTGTTGT	151.19514
BB93441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.77933	WGS84	BB93441	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93441_Mgig_Bot2012_COI	GD74	1	BB93441_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93441	Sample	BB93441	targeted	Absent	Sample	Mgig_Bot2012_COI	GD74	COI	45	BB93441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD74	TCTTATTCGTTGGAGACTTTATAACCCT	151.19654
BB93442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78083	WGS84	BB93442	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93442_Cmae_Bot2011_COI	GD76	1	BB93442_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93442	Sample	BB93442	targeted	Absent	Sample	Cmae_Bot2011_COI	GD76	COI	45	BB93442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD76	ATGAACAGTCTATCCTCCTTTAG	151.1992
BB93442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78083	WGS84	BB93442	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93442_Msal_Bot2012_COI	GD76	1	BB93442_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93442	Sample	BB93442	targeted	Absent	Sample	Msal_Bot2012_COI	GD76	COI	45	BB93442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD76	GYTAGTTCCRATGATGTTAGCTG	151.1992
BB93444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78485	WGS84	BB93444	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93444_Aamu_Bax2006_COI	GD79	1	BB93444_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93444	Sample	BB93444	targeted	Absent	Sample	Aamu_Bax2006_COI	GD79	COI	45	BB93444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD79	GCACAACCGGGATCTTTACTTC	151.20342
BB93444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78485	WGS84	BB93444	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93444_Pcan_Bot2011_IGS	GD79	1	BB93444_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93444	Sample	BB93444	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD79	IGS	45	BB93444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD79	CGTAATCCTCAGTACTGGCTA	151.20342
BB93444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78485	WGS84	BB93444	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93444_Sspa_Oph2007_COI	GD79	1	BB93444_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93444	Sample	BB93444	targeted	Absent	Sample	Sspa_Oph2007_COI	GD79	COI	45	BB93444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD79	GAAGGCCTTCCGATGATC	151.20342
BB93444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78485	WGS84	BB93444	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93444_Upin_Bot2011_COI	GD79	1	BB93444_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93444	Sample	BB93444	targeted	Absent	Sample	Upin_Bot2011_COI	GD79	COI	45	BB93444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD79	CTTTAATTACAGCGTTTTTATTGTTGT	151.20342
BB93445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79192	WGS84	BB93445	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93445_Mgig_Bot2012_COI	GD82	1	BB93445_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93445	Sample	BB93445	targeted	Absent	Sample	Mgig_Bot2012_COI	GD82	COI	45	BB93445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD82	TCTTATTCGTTGGAGACTTTATAACCCT	151.21144
BB93446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79468	WGS84	BB93446	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93446_Cmae_Bot2011_COI	GD84	1	BB93446_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93446	Sample	BB93446	targeted	Absent	Sample	Cmae_Bot2011_COI	GD84	COI	45	BB93446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD84	ATGAACAGTCTATCCTCCTTTAG	151.21374
BB93446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79468	WGS84	BB93446	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93446_Msal_Bot2012_COI	GD84	1	BB93446_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93446	Sample	BB93446	targeted	Absent	Sample	Msal_Bot2012_COI	GD84	COI	45	BB93446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD84	GYTAGTTCCRATGATGTTAGCTG	151.21374
BB93448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85314	WGS84	BB93448	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93448_Aamu_Bax2006_COI	GD88	1	BB93448_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93448	Sample	BB93448	targeted	Absent	Sample	Aamu_Bax2006_COI	GD88	COI	45	BB93448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD88	GCACAACCGGGATCTTTACTTC	151.31966
BB93448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85314	WGS84	BB93448	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93448_Pcan_Bot2011_IGS	GD88	1	BB93448_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93448	Sample	BB93448	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD88	IGS	45	BB93448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD88	CGTAATCCTCAGTACTGGCTA	151.31966
BB93448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85314	WGS84	BB93448	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93448_Sspa_Oph2007_COI	GD88	1	BB93448_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93448	Sample	BB93448	targeted	Absent	Sample	Sspa_Oph2007_COI	GD88	COI	45	BB93448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD88	GAAGGCCTTCCGATGATC	151.31966
BB93448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85314	WGS84	BB93448	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93448_Upin_Bot2011_COI	GD88	1	BB93448_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93448	Sample	BB93448	targeted	Absent	Sample	Upin_Bot2011_COI	GD88	COI	45	BB93448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD88	CTTTAATTACAGCGTTTTTATTGTTGT	151.31966
BB93449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8526	WGS84	BB93449	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93449_Mgig_Bot2012_COI	GD91	1	BB93449_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93449	Sample	BB93449	targeted	Absent	Sample	Mgig_Bot2012_COI	GD91	COI	45	BB93449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD91	TCTTATTCGTTGGAGACTTTATAACCCT	151.31603
BB93451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85305	WGS84	BB93451	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93451_Aamu_Bax2006_COI	GD97	1	BB93451_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93451	Sample	BB93451	targeted	Absent	Sample	Aamu_Bax2006_COI	GD97	COI	45	BB93451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD97	GCACAACCGGGATCTTTACTTC	151.3086
BB93451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85305	WGS84	BB93451	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93451_Pcan_Bot2011_IGS	GD97	1	BB93451_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93451	Sample	BB93451	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD97	IGS	45	BB93451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD97	CGTAATCCTCAGTACTGGCTA	151.3086
BB93451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85305	WGS84	BB93451	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93451_Sspa_Oph2007_COI	GD97	1	BB93451_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93451	Sample	BB93451	targeted	Absent	Sample	Sspa_Oph2007_COI	GD97	COI	45	BB93451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD97	GAAGGCCTTCCGATGATC	151.3086
BB93451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85305	WGS84	BB93451	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93451_Upin_Bot2011_COI	GD97	1	BB93451_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93451	Sample	BB93451	targeted	Absent	Sample	Upin_Bot2011_COI	GD97	COI	45	BB93451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD97	CTTTAATTACAGCGTTTTTATTGTTGT	151.3086
BB93452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81936	WGS84	BB93452	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93452_Mgig_Bot2012_COI	GD10	1	BB93452_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93452	Sample	BB93452	targeted	Absent	Sample	Mgig_Bot2012_COI	GD10	COI	45	BB93452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD10	TCTTATTCGTTGGAGACTTTATAACCCT	151.24067
BB93453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82128	WGS84	BB93453	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93453_Cmae_Bot2011_COI	GD15	1	BB93453_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93453	Sample	BB93453	targeted	Absent	Sample	Cmae_Bot2011_COI	GD15	COI	45	BB93453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD15	ATGAACAGTCTATCCTCCTTTAG	151.24058
BB93453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82128	WGS84	BB93453	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93453_Msal_Bot2012_COI	GD15	1	BB93453_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93453	Sample	BB93453	targeted	Absent	Sample	Msal_Bot2012_COI	GD15	COI	45	BB93453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD15	GYTAGTTCCRATGATGTTAGCTG	151.24058
BB93455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82269	WGS84	BB93455	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93455_Aamu_Bax2006_COI	GD25	1	BB93455_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93455	Sample	BB93455	targeted	Absent	Sample	Aamu_Bax2006_COI	GD25	COI	45	BB93455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD25	GCACAACCGGGATCTTTACTTC	151.24141
BB93455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82269	WGS84	BB93455	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93455_Pcan_Bot2011_IGS	GD25	1	BB93455_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93455	Sample	BB93455	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD25	IGS	45	BB93455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD25	CGTAATCCTCAGTACTGGCTA	151.24141
BB93455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82269	WGS84	BB93455	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93455_Sspa_Oph2007_COI	GD25	1	BB93455_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93455	Sample	BB93455	targeted	Absent	Sample	Sspa_Oph2007_COI	GD25	COI	45	BB93455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD25	GAAGGCCTTCCGATGATC	151.24141
BB93455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82269	WGS84	BB93455	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93455_Upin_Bot2011_COI	GD25	1	BB93455_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93455	Sample	BB93455	targeted	Absent	Sample	Upin_Bot2011_COI	GD25	COI	45	BB93455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD25	CTTTAATTACAGCGTTTTTATTGTTGT	151.24141
BB93456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82442	WGS84	BB93456	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93456_Mgig_Bot2012_COI	GD30	1	BB93456_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93456	Sample	BB93456	targeted	Absent	Sample	Mgig_Bot2012_COI	GD30	COI	45	BB93456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD30	TCTTATTCGTTGGAGACTTTATAACCCT	151.24865
BB93457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82366	WGS84	BB93457	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93457_Cmae_Bot2011_COI	GD105	1	BB93457_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93457	Sample	BB93457	targeted	Absent	Sample	Cmae_Bot2011_COI	GD105	COI	45	BB93457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD105	ATGAACAGTCTATCCTCCTTTAG	151.24798
BB93457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82366	WGS84	BB93457	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93457_Msal_Bot2012_COI	GD105	1	BB93457_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93457	Sample	BB93457	targeted	Absent	Sample	Msal_Bot2012_COI	GD105	COI	45	BB93457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD105	GYTAGTTCCRATGATGTTAGCTG	151.24798
BB93459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83496	WGS84	BB93459	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93459_Aamu_Bax2006_COI	GD40	1	BB93459_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93459	Sample	BB93459	targeted	Absent	Sample	Aamu_Bax2006_COI	GD40	COI	45	BB93459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD40	GCACAACCGGGATCTTTACTTC	151.26835
BB93459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83496	WGS84	BB93459	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93459_Pcan_Bot2011_IGS	GD40	1	BB93459_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93459	Sample	BB93459	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD40	IGS	45	BB93459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD40	CGTAATCCTCAGTACTGGCTA	151.26835
BB93459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83496	WGS84	BB93459	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93459_Sspa_Oph2007_COI	GD40	1	BB93459_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93459	Sample	BB93459	targeted	Absent	Sample	Sspa_Oph2007_COI	GD40	COI	45	BB93459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD40	GAAGGCCTTCCGATGATC	151.26835
BB93459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83496	WGS84	BB93459	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93459_Upin_Bot2011_COI	GD40	1	BB93459_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93459	Sample	BB93459	targeted	Absent	Sample	Upin_Bot2011_COI	GD40	COI	45	BB93459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD40	CTTTAATTACAGCGTTTTTATTGTTGT	151.26835
BB93460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83723	WGS84	BB93460	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93460_Cmae_Bot2011_COI	GD100	1	BB93460_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93460	Sample	BB93460	targeted	Absent	Sample	Cmae_Bot2011_COI	GD100	COI	45	BB93460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD100	ATGAACAGTCTATCCTCCTTTAG	151.27246
BB93460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83723	WGS84	BB93460	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93460_Msal_Bot2012_COI	GD100	1	BB93460_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93460	Sample	BB93460	targeted	Absent	Sample	Msal_Bot2012_COI	GD100	COI	45	BB93460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD100	GYTAGTTCCRATGATGTTAGCTG	151.27246
BB93462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82706	WGS84	BB93462	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93462_Aamu_Bax2006_COI	GD1	1	BB93462_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93462	Sample	BB93462	targeted	Absent	Sample	Aamu_Bax2006_COI	GD1	COI	45	BB93462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD1	GCACAACCGGGATCTTTACTTC	151.24741
BB93462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82706	WGS84	BB93462	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93462_Pcan_Bot2011_IGS	GD1	1	BB93462_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93462	Sample	BB93462	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD1	IGS	45	BB93462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD1	CGTAATCCTCAGTACTGGCTA	151.24741
BB93462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82706	WGS84	BB93462	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93462_Sspa_Oph2007_COI	GD1	1	BB93462_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93462	Sample	BB93462	targeted	Absent	Sample	Sspa_Oph2007_COI	GD1	COI	45	BB93462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD1	GAAGGCCTTCCGATGATC	151.24741
BB93462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82706	WGS84	BB93462	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93462_Upin_Bot2011_COI	GD1	1	BB93462_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93462	Sample	BB93462	targeted	Absent	Sample	Upin_Bot2011_COI	GD1	COI	45	BB93462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD1	CTTTAATTACAGCGTTTTTATTGTTGT	151.24741
BB93463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82693	WGS84	BB93463	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93463_Mgig_Bot2012_COI	GD2	1	BB93463_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93463	Sample	BB93463	targeted	Absent	Sample	Mgig_Bot2012_COI	GD2	COI	45	BB93463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD2	TCTTATTCGTTGGAGACTTTATAACCCT	151.24805
BB93464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81622	WGS84	BB93464	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93464_Cmae_Bot2011_COI	GD3	1	BB93464_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93464	Sample	BB93464	targeted	Absent	Sample	Cmae_Bot2011_COI	GD3	COI	45	BB93464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD3	ATGAACAGTCTATCCTCCTTTAG	151.23706
BB93464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81622	WGS84	BB93464	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93464_Msal_Bot2012_COI	GD3	1	BB93464_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93464	Sample	BB93464	targeted	Absent	Sample	Msal_Bot2012_COI	GD3	COI	45	BB93464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD3	GYTAGTTCCRATGATGTTAGCTG	151.23706
BB93466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8178	WGS84	BB93466	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93466_Aamu_Bax2006_COI	GD7	1	BB93466_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93466	Sample	BB93466	targeted	Absent	Sample	Aamu_Bax2006_COI	GD7	COI	45	BB93466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD7	GCACAACCGGGATCTTTACTTC	151.23909
BB93466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8178	WGS84	BB93466	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93466_Pcan_Bot2011_IGS	GD7	1	BB93466_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93466	Sample	BB93466	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD7	IGS	45	BB93466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD7	CGTAATCCTCAGTACTGGCTA	151.23909
BB93466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8178	WGS84	BB93466	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93466_Sspa_Oph2007_COI	GD7	1	BB93466_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93466	Sample	BB93466	targeted	Absent	Sample	Sspa_Oph2007_COI	GD7	COI	45	BB93466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD7	GAAGGCCTTCCGATGATC	151.23909
BB93466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8178	WGS84	BB93466	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93466_Upin_Bot2011_COI	GD7	1	BB93466_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93466	Sample	BB93466	targeted	Absent	Sample	Upin_Bot2011_COI	GD7	COI	45	BB93466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD7	CTTTAATTACAGCGTTTTTATTGTTGT	151.23909
BB93467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81887	WGS84	BB93467	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93467_Mgig_Bot2012_COI	GD9	1	BB93467_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93467	Sample	BB93467	targeted	Absent	Sample	Mgig_Bot2012_COI	GD9	COI	45	BB93467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD9	TCTTATTCGTTGGAGACTTTATAACCCT	151.23964
BB93468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82046	WGS84	BB93468	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93468_Cmae_Bot2011_COI	GD12	1	BB93468_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93468	Sample	BB93468	targeted	Absent	Sample	Cmae_Bot2011_COI	GD12	COI	45	BB93468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD12	ATGAACAGTCTATCCTCCTTTAG	151.23915
BB93468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82046	WGS84	BB93468	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93468_Msal_Bot2012_COI	GD12	1	BB93468_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93468	Sample	BB93468	targeted	Absent	Sample	Msal_Bot2012_COI	GD12	COI	45	BB93468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD12	GYTAGTTCCRATGATGTTAGCTG	151.23915
BB93470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81992	WGS84	BB93470	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93470_Mgig_Bot2012_COI	GD17	1	BB93470_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93470	Sample	BB93470	targeted	Absent	Sample	Mgig_Bot2012_COI	GD17	COI	45	BB93470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD17	TCTTATTCGTTGGAGACTTTATAACCCT	151.24107
BB93471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82143	WGS84	BB93471	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93471_Cmae_Bot2011_COI	GD19	1	BB93471_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93471	Sample	BB93471	targeted	Absent	Sample	Cmae_Bot2011_COI	GD19	COI	45	BB93471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD19	ATGAACAGTCTATCCTCCTTTAG	151.24285
BB93471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82143	WGS84	BB93471	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93471_Msal_Bot2012_COI	GD19	1	BB93471_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93471	Sample	BB93471	targeted	Absent	Sample	Msal_Bot2012_COI	GD19	COI	45	BB93471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD19	GYTAGTTCCRATGATGTTAGCTG	151.24285
BB93473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82382	WGS84	BB93473	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93473_Aamu_Bax2006_COI	GD27	1	BB93473_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93473	Sample	BB93473	targeted	Absent	Sample	Aamu_Bax2006_COI	GD27	COI	45	BB93473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD27	GCACAACCGGGATCTTTACTTC	151.24513
BB93473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82382	WGS84	BB93473	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93473_Pcan_Bot2011_IGS	GD27	1	BB93473_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93473	Sample	BB93473	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD27	IGS	45	BB93473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD27	CGTAATCCTCAGTACTGGCTA	151.24513
BB93473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82382	WGS84	BB93473	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93473_Sspa_Oph2007_COI	GD27	1	BB93473_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93473	Sample	BB93473	targeted	Absent	Sample	Sspa_Oph2007_COI	GD27	COI	45	BB93473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD27	GAAGGCCTTCCGATGATC	151.24513
BB93473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82382	WGS84	BB93473	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93473_Upin_Bot2011_COI	GD27	1	BB93473_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93473	Sample	BB93473	targeted	Absent	Sample	Upin_Bot2011_COI	GD27	COI	45	BB93473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD27	CTTTAATTACAGCGTTTTTATTGTTGT	151.24513
BB93474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82492	WGS84	BB93474	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93474_Mgig_Bot2012_COI	GD28	1	BB93474_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93474	Sample	BB93474	targeted	Absent	Sample	Mgig_Bot2012_COI	GD28	COI	45	BB93474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD28	TCTTATTCGTTGGAGACTTTATAACCCT	151.24519
BB93475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82536	WGS84	BB93475	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93475_Cmae_Bot2011_COI	GD32	1	BB93475_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93475	Sample	BB93475	targeted	Absent	Sample	Cmae_Bot2011_COI	GD32	COI	45	BB93475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD32	ATGAACAGTCTATCCTCCTTTAG	151.24636
BB93475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82536	WGS84	BB93475	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93475_Msal_Bot2012_COI	GD32	1	BB93475_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93475	Sample	BB93475	targeted	Absent	Sample	Msal_Bot2012_COI	GD32	COI	45	BB93475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD32	GYTAGTTCCRATGATGTTAGCTG	151.24636
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.702	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93477_Aamu_Bax2006_COI	MD125	1	BB93477_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Present	Sample	Aamu_Bax2006_COI	MD125	COI	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	GCACAACCGGGATCTTTACTTC	144.90157
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93477_Pcan_Bot2011_IGS	MD125	1	BB93477_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD125	IGS	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	CGTAATCCTCAGTACTGGCTA	144.90157
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.338	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93477_Sspa_Oph2007_COI	MD125	1	BB93477_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Present	Sample	Sspa_Oph2007_COI	MD125	COI	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	GAAGGCCTTCCGATGATC	144.90157
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93477_Upin_Bot2011_COI	MD125	1	BB93477_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Absent	Sample	Upin_Bot2011_COI	MD125	COI	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	CTTTAATTACAGCGTTTTTATTGTTGT	144.90157
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93478_Mgig_Bot2012_COI	MD120	1	BB93478_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Absent	Sample	Mgig_Bot2012_COI	MD120	COI	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	TCTTATTCGTTGGAGACTTTATAACCCT	144.90171
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93479_Cmae_Bot2011_COI	MD115	1	BB93479_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Absent	Sample	Cmae_Bot2011_COI	MD115	COI	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	ATGAACAGTCTATCCTCCTTTAG	144.90722
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93479_Hsan_Wil2021_COI	MD115	1	BB93479_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Absent	Sample	Hsan_Wil2021_COI	MD115	COI	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	CCTGGGCCGGTATAGTAGGT	144.90722
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93479_Msal_Bot2012_COI	MD115	1	BB93479_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Absent	Sample	Msal_Bot2012_COI	MD115	COI	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	GYTAGTTCCRATGATGTTAGCTG	144.90722
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.784	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93480_Aamu_Bax2006_COI	MD105	1	BB93480_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Present	Sample	Aamu_Bax2006_COI	MD105	COI	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	GCACAACCGGGATCTTTACTTC	144.91047
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93480_Pcan_Bot2011_IGS	MD105	1	BB93480_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD105	IGS	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	CGTAATCCTCAGTACTGGCTA	144.91047
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.207	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93480_Sspa_Oph2007_COI	MD105	1	BB93480_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Present	Sample	Sspa_Oph2007_COI	MD105	COI	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	GAAGGCCTTCCGATGATC	144.91047
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93480_Upin_Bot2011_COI	MD105	1	BB93480_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Absent	Sample	Upin_Bot2011_COI	MD105	COI	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	CTTTAATTACAGCGTTTTTATTGTTGT	144.91047
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93481_Mgig_Bot2012_COI	MD100	1	BB93481_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Absent	Sample	Mgig_Bot2012_COI	MD100	COI	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	TCTTATTCGTTGGAGACTTTATAACCCT	144.91348
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93482_Cmae_Bot2011_COI	MD90	1	BB93482_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Absent	Sample	Cmae_Bot2011_COI	MD90	COI	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	ATGAACAGTCTATCCTCCTTTAG	144.91436
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93482_Hsan_Wil2021_COI	MD90	1	BB93482_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Absent	Sample	Hsan_Wil2021_COI	MD90	COI	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	CCTGGGCCGGTATAGTAGGT	144.91436
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93482_Msal_Bot2012_COI	MD90	1	BB93482_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Absent	Sample	Msal_Bot2012_COI	MD90	COI	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	GYTAGTTCCRATGATGTTAGCTG	144.91436
BB93484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81957	WGS84	BB93484	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.09	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93484_Aamu_Bax2006_COI	MD80	1	BB93484_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93484	Sample	BB93484	targeted	Present	Sample	Aamu_Bax2006_COI	MD80	COI	45	BB93484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD80	GCACAACCGGGATCTTTACTTC	144.91825
BB93484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81957	WGS84	BB93484	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93484_Pcan_Bot2011_IGS	MD80	1	BB93484_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93484	Sample	BB93484	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD80	IGS	45	BB93484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD80	CGTAATCCTCAGTACTGGCTA	144.91825
BB93484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81957	WGS84	BB93484	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.304	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93484_Sspa_Oph2007_COI	MD80	1	BB93484_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93484	Sample	BB93484	targeted	Present	Sample	Sspa_Oph2007_COI	MD80	COI	45	BB93484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD80	GAAGGCCTTCCGATGATC	144.91825
BB93484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81957	WGS84	BB93484	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93484_Upin_Bot2011_COI	MD80	1	BB93484_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93484	Sample	BB93484	targeted	Absent	Sample	Upin_Bot2011_COI	MD80	COI	45	BB93484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD80	CTTTAATTACAGCGTTTTTATTGTTGT	144.91825
BB93485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81669	WGS84	BB93485	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93485_Mgig_Bot2012_COI	MD70	1	BB93485_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93485	Sample	BB93485	targeted	Absent	Sample	Mgig_Bot2012_COI	MD70	COI	45	BB93485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD70	TCTTATTCGTTGGAGACTTTATAACCCT	144.92406
BB93486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81858	WGS84	BB93486	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93486_Cmae_Bot2011_COI	MD65	1	BB93486_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93486	Sample	BB93486	targeted	Absent	Sample	Cmae_Bot2011_COI	MD65	COI	45	BB93486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD65	ATGAACAGTCTATCCTCCTTTAG	144.92668
BB93486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81858	WGS84	BB93486	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93486_Msal_Bot2012_COI	MD65	1	BB93486_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93486	Sample	BB93486	targeted	Absent	Sample	Msal_Bot2012_COI	MD65	COI	45	BB93486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD65	GYTAGTTCCRATGATGTTAGCTG	144.92668
BB93488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86098	WGS84	BB93488	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.029	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93488_Aamu_Bax2006_COI	MD55	1	BB93488_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93488	Sample	BB93488	targeted	Present	Sample	Aamu_Bax2006_COI	MD55	COI	45	BB93488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD55	GCACAACCGGGATCTTTACTTC	144.90725
BB93488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86098	WGS84	BB93488	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93488_Pcan_Bot2011_IGS	MD55	1	BB93488_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93488	Sample	BB93488	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD55	IGS	45	BB93488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD55	CGTAATCCTCAGTACTGGCTA	144.90725
BB93488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86098	WGS84	BB93488	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.823	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93488_Sspa_Oph2007_COI	MD55	1	BB93488_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93488	Sample	BB93488	targeted	Present	Sample	Sspa_Oph2007_COI	MD55	COI	45	BB93488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD55	GAAGGCCTTCCGATGATC	144.90725
BB93488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86098	WGS84	BB93488	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.592	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93488_Upin_Bot2011_COI	MD55	1	BB93488_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93488	Sample	BB93488	targeted	Present	Sample	Upin_Bot2011_COI	MD55	COI	45	BB93488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD55	CTTTAATTACAGCGTTTTTATTGTTGT	144.90725
BB93489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93489	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93489_Mgig_Bot2012_COI	MD45	1	BB93489_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93489	Sample	BB93489	targeted	Absent	Sample	Mgig_Bot2012_COI	MD45	COI	45	BB93489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD45	TCTTATTCGTTGGAGACTTTATAACCCT	144.91313
BB93491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86484	WGS84	BB93491	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.091	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93491_Aamu_Bax2006_COI	MD30	1	BB93491_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93491	Sample	BB93491	targeted	Present	Sample	Aamu_Bax2006_COI	MD30	COI	45	BB93491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD30	GCACAACCGGGATCTTTACTTC	144.91759
BB93491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86484	WGS84	BB93491	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93491_Pcan_Bot2011_IGS	MD30	1	BB93491_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93491	Sample	BB93491	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD30	IGS	45	BB93491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD30	CGTAATCCTCAGTACTGGCTA	144.91759
BB93491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86484	WGS84	BB93491	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.049	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93491_Sspa_Oph2007_COI	MD30	1	BB93491_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93491	Sample	BB93491	targeted	Present	Sample	Sspa_Oph2007_COI	MD30	COI	45	BB93491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD30	GAAGGCCTTCCGATGATC	144.91759
BB93491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86484	WGS84	BB93491	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.432	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93491_Upin_Bot2011_COI	MD30	1	BB93491_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93491	Sample	BB93491	targeted	Present	Sample	Upin_Bot2011_COI	MD30	COI	45	BB93491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD30	CTTTAATTACAGCGTTTTTATTGTTGT	144.91759
BB93492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8467	WGS84	BB93492	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93492_Mgig_Bot2012_COI	MD20	1	BB93492_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93492	Sample	BB93492	targeted	Absent	Sample	Mgig_Bot2012_COI	MD20	COI	45	BB93492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD20	TCTTATTCGTTGGAGACTTTATAACCCT	144.90834
BB93493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82706	WGS84	BB93493	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93493_Cmae_Bot2011_COI	MD124	1	BB93493_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93493	Sample	BB93493	targeted	Absent	Sample	Cmae_Bot2011_COI	MD124	COI	45	BB93493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD124	ATGAACAGTCTATCCTCCTTTAG	144.89997
BB93493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82706	WGS84	BB93493	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93493_Msal_Bot2012_COI	MD124	1	BB93493_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93493	Sample	BB93493	targeted	Absent	Sample	Msal_Bot2012_COI	MD124	COI	45	BB93493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD124	GYTAGTTCCRATGATGTTAGCTG	144.89997
BB93495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8245	WGS84	BB93495	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.446	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93495_Aamu_Bax2006_COI	MD119	1	BB93495_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93495	Sample	BB93495	targeted	Present	Sample	Aamu_Bax2006_COI	MD119	COI	45	BB93495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD119	GCACAACCGGGATCTTTACTTC	144.904
BB93495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8245	WGS84	BB93495	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93495_Pcan_Bot2011_IGS	MD119	1	BB93495_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93495	Sample	BB93495	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD119	IGS	45	BB93495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD119	CGTAATCCTCAGTACTGGCTA	144.904
BB93495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8245	WGS84	BB93495	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.058	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93495_Sspa_Oph2007_COI	MD119	1	BB93495_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93495	Sample	BB93495	targeted	Present	Sample	Sspa_Oph2007_COI	MD119	COI	45	BB93495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD119	GAAGGCCTTCCGATGATC	144.904
BB93495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8245	WGS84	BB93495	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93495_Upin_Bot2011_COI	MD119	1	BB93495_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93495	Sample	BB93495	targeted	Absent	Sample	Upin_Bot2011_COI	MD119	COI	45	BB93495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD119	CTTTAATTACAGCGTTTTTATTGTTGT	144.904
BB93496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82348	WGS84	BB93496	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93496_Mgig_Bot2012_COI	MD117	1	BB93496_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93496	Sample	BB93496	targeted	Absent	Sample	Mgig_Bot2012_COI	MD117	COI	45	BB93496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD117	TCTTATTCGTTGGAGACTTTATAACCCT	144.90236
BB93497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8209	WGS84	BB93497	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93497_Cmae_Bot2011_COI	MD113	1	BB93497_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93497	Sample	BB93497	targeted	Absent	Sample	Cmae_Bot2011_COI	MD113	COI	45	BB93497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD113	ATGAACAGTCTATCCTCCTTTAG	144.90658
BB93497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8209	WGS84	BB93497	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93497_Msal_Bot2012_COI	MD113	1	BB93497_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93497	Sample	BB93497	targeted	Absent	Sample	Msal_Bot2012_COI	MD113	COI	45	BB93497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD113	GYTAGTTCCRATGATGTTAGCTG	144.90658
BB93499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82141	WGS84	BB93499	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.649	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93499_Aamu_Bax2006_COI	MD108	1	BB93499_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93499	Sample	BB93499	targeted	Present	Sample	Aamu_Bax2006_COI	MD108	COI	45	BB93499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD108	GCACAACCGGGATCTTTACTTC	144.90788
BB93499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82141	WGS84	BB93499	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93499_Pcan_Bot2011_IGS	MD108	1	BB93499_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93499	Sample	BB93499	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD108	IGS	45	BB93499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD108	CGTAATCCTCAGTACTGGCTA	144.90788
BB93499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82141	WGS84	BB93499	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.491	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93499_Sspa_Oph2007_COI	MD108	1	BB93499_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93499	Sample	BB93499	targeted	Present	Sample	Sspa_Oph2007_COI	MD108	COI	45	BB93499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD108	GAAGGCCTTCCGATGATC	144.90788
BB93499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82141	WGS84	BB93499	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93499_Upin_Bot2011_COI	MD108	1	BB93499_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93499	Sample	BB93499	targeted	Absent	Sample	Upin_Bot2011_COI	MD108	COI	45	BB93499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD108	CTTTAATTACAGCGTTTTTATTGTTGT	144.90788
BB93500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82007	WGS84	BB93500	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93500_Mgig_Bot2012_COI	MD107	1	BB93500_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93500	Sample	BB93500	targeted	Absent	Sample	Mgig_Bot2012_COI	MD107	COI	45	BB93500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD107	TCTTATTCGTTGGAGACTTTATAACCCT	144.90792
BB93501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82138	WGS84	BB93501	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93501_Cmae_Bot2011_COI	MD106	1	BB93501_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93501	Sample	BB93501	targeted	Absent	Sample	Cmae_Bot2011_COI	MD106	COI	45	BB93501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD106	ATGAACAGTCTATCCTCCTTTAG	144.90934
BB93501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82138	WGS84	BB93501	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93501_Msal_Bot2012_COI	MD106	1	BB93501_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93501	Sample	BB93501	targeted	Absent	Sample	Msal_Bot2012_COI	MD106	COI	45	BB93501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD106	GYTAGTTCCRATGATGTTAGCTG	144.90934
BB93503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8201	WGS84	BB93503	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.807	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93503_Aamu_Bax2006_COI	MD101	1	BB93503_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93503	Sample	BB93503	targeted	Present	Sample	Aamu_Bax2006_COI	MD101	COI	45	BB93503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD101	GCACAACCGGGATCTTTACTTC	144.91155
BB93503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8201	WGS84	BB93503	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93503_Pcan_Bot2011_IGS	MD101	1	BB93503_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93503	Sample	BB93503	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD101	IGS	45	BB93503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD101	CGTAATCCTCAGTACTGGCTA	144.91155
BB93503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8201	WGS84	BB93503	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.394	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93503_Sspa_Oph2007_COI	MD101	1	BB93503_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93503	Sample	BB93503	targeted	Present	Sample	Sspa_Oph2007_COI	MD101	COI	45	BB93503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD101	GAAGGCCTTCCGATGATC	144.91155
BB93503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8201	WGS84	BB93503	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93503_Upin_Bot2011_COI	MD101	1	BB93503_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93503	Sample	BB93503	targeted	Absent	Sample	Upin_Bot2011_COI	MD101	COI	45	BB93503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD101	CTTTAATTACAGCGTTTTTATTGTTGT	144.91155
BB93504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93504	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93504_Mgig_Bot2012_COI	MD98	1	BB93504_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93504	Sample	BB93504	targeted	Absent	Sample	Mgig_Bot2012_COI	MD98	COI	45	BB93504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD98	TCTTATTCGTTGGAGACTTTATAACCCT	144.91364
BB93505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81014	WGS84	BB93505	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93505_Cmae_Bot2011_COI	MD96	1	BB93505_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93505	Sample	BB93505	targeted	Absent	Sample	Cmae_Bot2011_COI	MD96	COI	45	BB93505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD96	ATGAACAGTCTATCCTCCTTTAG	144.91453
BB93505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81014	WGS84	BB93505	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93505_Msal_Bot2012_COI	MD96	1	BB93505_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93505	Sample	BB93505	targeted	Absent	Sample	Msal_Bot2012_COI	MD96	COI	45	BB93505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD96	GYTAGTTCCRATGATGTTAGCTG	144.91453
BB93507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81165	WGS84	BB93507	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.712	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93507_Aamu_Bax2006_COI	MD93	1	BB93507_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93507	Sample	BB93507	targeted	Present	Sample	Aamu_Bax2006_COI	MD93	COI	45	BB93507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD93	GCACAACCGGGATCTTTACTTC	144.91297
BB93507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81165	WGS84	BB93507	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93507_Pcan_Bot2011_IGS	MD93	1	BB93507_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93507	Sample	BB93507	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD93	IGS	45	BB93507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD93	CGTAATCCTCAGTACTGGCTA	144.91297
BB93507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81165	WGS84	BB93507	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.268	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93507_Sspa_Oph2007_COI	MD93	1	BB93507_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93507	Sample	BB93507	targeted	Present	Sample	Sspa_Oph2007_COI	MD93	COI	45	BB93507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD93	GAAGGCCTTCCGATGATC	144.91297
BB93507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81165	WGS84	BB93507	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93507_Upin_Bot2011_COI	MD93	1	BB93507_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93507	Sample	BB93507	targeted	Absent	Sample	Upin_Bot2011_COI	MD93	COI	45	BB93507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD93	CTTTAATTACAGCGTTTTTATTGTTGT	144.91297
BB93508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81445	WGS84	BB93508	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93508_Mgig_Bot2012_COI	MD91	1	BB93508_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93508	Sample	BB93508	targeted	Absent	Sample	Mgig_Bot2012_COI	MD91	COI	45	BB93508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD91	TCTTATTCGTTGGAGACTTTATAACCCT	144.91369
BB93509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81584	WGS84	BB93509	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93509_Cmae_Bot2011_COI	MD88	1	BB93509_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93509	Sample	BB93509	targeted	Absent	Sample	Cmae_Bot2011_COI	MD88	COI	45	BB93509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD88	ATGAACAGTCTATCCTCCTTTAG	144.91397
BB93509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81584	WGS84	BB93509	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93509_Msal_Bot2012_COI	MD88	1	BB93509_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93509	Sample	BB93509	targeted	Absent	Sample	Msal_Bot2012_COI	MD88	COI	45	BB93509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD88	GYTAGTTCCRATGATGTTAGCTG	144.91397
BB93510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81717	WGS84	BB93510	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.223	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93510_Aamu_Bax2006_COI	MD86	1	BB93510_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93510	Sample	BB93510	targeted	Present	Sample	Aamu_Bax2006_COI	MD86	COI	45	BB93510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD86	GCACAACCGGGATCTTTACTTC	144.91329
BB93510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81717	WGS84	BB93510	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93510_Pcan_Bot2011_IGS	MD86	1	BB93510_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93510	Sample	BB93510	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD86	IGS	45	BB93510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD86	CGTAATCCTCAGTACTGGCTA	144.91329
BB93510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81717	WGS84	BB93510	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	26.427	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93510_Sspa_Oph2007_COI	MD86	1	BB93510_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93510	Sample	BB93510	targeted	Present	Sample	Sspa_Oph2007_COI	MD86	COI	45	BB93510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD86	GAAGGCCTTCCGATGATC	144.91329
BB93510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81717	WGS84	BB93510	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93510_Upin_Bot2011_COI	MD86	1	BB93510_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93510	Sample	BB93510	targeted	Absent	Sample	Upin_Bot2011_COI	MD86	COI	45	BB93510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD86	CTTTAATTACAGCGTTTTTATTGTTGT	144.91329
BB93511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81836	WGS84	BB93511	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93511_Mgig_Bot2012_COI	MD83	1	BB93511_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93511	Sample	BB93511	targeted	Absent	Sample	Mgig_Bot2012_COI	MD83	COI	45	BB93511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD83	TCTTATTCGTTGGAGACTTTATAACCCT	144.91492
BB93512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81938	WGS84	BB93512	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93512_Cmae_Bot2011_COI	MD82	1	BB93512_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93512	Sample	BB93512	targeted	Absent	Sample	Cmae_Bot2011_COI	MD82	COI	45	BB93512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD82	ATGAACAGTCTATCCTCCTTTAG	144.91696
BB93512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81938	WGS84	BB93512	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93512_Msal_Bot2012_COI	MD82	1	BB93512_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93512	Sample	BB93512	targeted	Absent	Sample	Msal_Bot2012_COI	MD82	COI	45	BB93512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD82	GYTAGTTCCRATGATGTTAGCTG	144.91696
BB93514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81923	WGS84	BB93514	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.421	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93514_Aamu_Bax2006_COI	MD77	1	BB93514_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93514	Sample	BB93514	targeted	Present	Sample	Aamu_Bax2006_COI	MD77	COI	45	BB93514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD77	GCACAACCGGGATCTTTACTTC	144.92092
BB93514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81923	WGS84	BB93514	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93514_Pcan_Bot2011_IGS	MD77	1	BB93514_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93514	Sample	BB93514	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD77	IGS	45	BB93514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD77	CGTAATCCTCAGTACTGGCTA	144.92092
BB93514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81923	WGS84	BB93514	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.133	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93514_Sspa_Oph2007_COI	MD77	1	BB93514_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93514	Sample	BB93514	targeted	Present	Sample	Sspa_Oph2007_COI	MD77	COI	45	BB93514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD77	GAAGGCCTTCCGATGATC	144.92092
BB93514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81923	WGS84	BB93514	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93514_Upin_Bot2011_COI	MD77	1	BB93514_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93514	Sample	BB93514	targeted	Absent	Sample	Upin_Bot2011_COI	MD77	COI	45	BB93514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD77	CTTTAATTACAGCGTTTTTATTGTTGT	144.92092
BB93515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81903	WGS84	BB93515	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93515_Mgig_Bot2012_COI	MD74	1	BB93515_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93515	Sample	BB93515	targeted	Absent	Sample	Mgig_Bot2012_COI	MD74	COI	45	BB93515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD74	TCTTATTCGTTGGAGACTTTATAACCCT	144.92243
BB93516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8197	WGS84	BB93516	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93516_Cmae_Bot2011_COI	MD73	1	BB93516_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93516	Sample	BB93516	targeted	Absent	Sample	Cmae_Bot2011_COI	MD73	COI	45	BB93516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD73	ATGAACAGTCTATCCTCCTTTAG	144.92558
BB93516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8197	WGS84	BB93516	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93516_Msal_Bot2012_COI	MD73	1	BB93516_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93516	Sample	BB93516	targeted	Absent	Sample	Msal_Bot2012_COI	MD73	COI	45	BB93516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD73	GYTAGTTCCRATGATGTTAGCTG	144.92558
BB93518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81672	WGS84	BB93518	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.488	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93518_Aamu_Bax2006_COI	MD68	1	BB93518_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93518	Sample	BB93518	targeted	Present	Sample	Aamu_Bax2006_COI	MD68	COI	45	BB93518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD68	GCACAACCGGGATCTTTACTTC	144.92453
BB93518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81672	WGS84	BB93518	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93518_Pcan_Bot2011_IGS	MD68	1	BB93518_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93518	Sample	BB93518	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD68	IGS	45	BB93518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD68	CGTAATCCTCAGTACTGGCTA	144.92453
BB93518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81672	WGS84	BB93518	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.338	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93518_Sspa_Oph2007_COI	MD68	1	BB93518_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93518	Sample	BB93518	targeted	Present	Sample	Sspa_Oph2007_COI	MD68	COI	45	BB93518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD68	GAAGGCCTTCCGATGATC	144.92453
BB93518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81672	WGS84	BB93518	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93518_Upin_Bot2011_COI	MD68	1	BB93518_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93518	Sample	BB93518	targeted	Absent	Sample	Upin_Bot2011_COI	MD68	COI	45	BB93518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD68	CTTTAATTACAGCGTTTTTATTGTTGT	144.92453
BB93519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8179	WGS84	BB93519	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93519_Mgig_Bot2012_COI	MD62	1	BB93519_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93519	Sample	BB93519	targeted	Absent	Sample	Mgig_Bot2012_COI	MD62	COI	45	BB93519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD62	TCTTATTCGTTGGAGACTTTATAACCCT	144.92486
BB93521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82012	WGS84	BB93521	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.029	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93521_Aamu_Bax2006_COI	MD59	1	BB93521_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93521	Sample	BB93521	targeted	Present	Sample	Aamu_Bax2006_COI	MD59	COI	45	BB93521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD59	GCACAACCGGGATCTTTACTTC	144.92885
BB93521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82012	WGS84	BB93521	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93521_Pcan_Bot2011_IGS	MD59	1	BB93521_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93521	Sample	BB93521	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD59	IGS	45	BB93521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD59	CGTAATCCTCAGTACTGGCTA	144.92885
BB93521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82012	WGS84	BB93521	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.164	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93521_Sspa_Oph2007_COI	MD59	1	BB93521_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93521	Sample	BB93521	targeted	Present	Sample	Sspa_Oph2007_COI	MD59	COI	45	BB93521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD59	GAAGGCCTTCCGATGATC	144.92885
BB93521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82012	WGS84	BB93521	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93521_Upin_Bot2011_COI	MD59	1	BB93521_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93521	Sample	BB93521	targeted	Absent	Sample	Upin_Bot2011_COI	MD59	COI	45	BB93521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD59	CTTTAATTACAGCGTTTTTATTGTTGT	144.92885
BB93522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93522	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.491	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93522_Mgig_Bot2012_COI	MD57	1	BB93522_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93522	Sample	BB93522	targeted	Present	Sample	Mgig_Bot2012_COI	MD57	COI	45	BB93522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD57	TCTTATTCGTTGGAGACTTTATAACCCT	144.93141
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93523_Cmae_Bot2011_COI	MD53	1	BB93523_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Absent	Sample	Cmae_Bot2011_COI	MD53	COI	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	ATGAACAGTCTATCCTCCTTTAG	144.91028
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93523_Hsan_Wil2021_COI	MD53	1	BB93523_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Absent	Sample	Hsan_Wil2021_COI	MD53	COI	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	CCTGGGCCGGTATAGTAGGT	144.91028
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93523_Msal_Bot2012_COI	MD53	1	BB93523_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Absent	Sample	Msal_Bot2012_COI	MD53	COI	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	GYTAGTTCCRATGATGTTAGCTG	144.91028
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.188	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93525_Aamu_Bax2006_COI	MD49	1	BB93525_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Present	Sample	Aamu_Bax2006_COI	MD49	COI	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	GCACAACCGGGATCTTTACTTC	144.91331
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93525_Pcan_Bot2011_IGS	MD49	1	BB93525_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD49	IGS	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	CGTAATCCTCAGTACTGGCTA	144.91331
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.699	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93525_Sspa_Oph2007_COI	MD49	1	BB93525_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Present	Sample	Sspa_Oph2007_COI	MD49	COI	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	GAAGGCCTTCCGATGATC	144.91331
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.424	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93525_Upin_Bot2011_COI	MD49	1	BB93525_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Present	Sample	Upin_Bot2011_COI	MD49	COI	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	CTTTAATTACAGCGTTTTTATTGTTGT	144.91331
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93526_Mgig_Bot2012_COI	MD48	1	BB93526_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Absent	Sample	Mgig_Bot2012_COI	MD48	COI	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	TCTTATTCGTTGGAGACTTTATAACCCT	144.91415
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.318	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93527_Cmae_Bot2011_COI	MD44	1	BB93527_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Present	Sample	Cmae_Bot2011_COI	MD44	COI	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	ATGAACAGTCTATCCTCCTTTAG	144.91391
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93527_Hsan_Wil2021_COI	MD44	1	BB93527_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Absent	Sample	Hsan_Wil2021_COI	MD44	COI	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	CCTGGGCCGGTATAGTAGGT	144.91391
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93527_Msal_Bot2012_COI	MD44	1	BB93527_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Absent	Sample	Msal_Bot2012_COI	MD44	COI	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	GYTAGTTCCRATGATGTTAGCTG	144.91391
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.252	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93529_Aamu_Bax2006_COI	MD39	1	BB93529_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Present	Sample	Aamu_Bax2006_COI	MD39	COI	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	GCACAACCGGGATCTTTACTTC	144.91853
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93529_Pcan_Bot2011_IGS	MD39	1	BB93529_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD39	IGS	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	CGTAATCCTCAGTACTGGCTA	144.91853
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.089	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93529_Sspa_Oph2007_COI	MD39	1	BB93529_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Present	Sample	Sspa_Oph2007_COI	MD39	COI	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	GAAGGCCTTCCGATGATC	144.91853
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.225	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93529_Upin_Bot2011_COI	MD39	1	BB93529_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Present	Sample	Upin_Bot2011_COI	MD39	COI	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	CTTTAATTACAGCGTTTTTATTGTTGT	144.91853
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93530_Cmae_Bot2011_COI	MD38	1	BB93530_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Absent	Sample	Cmae_Bot2011_COI	MD38	COI	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	ATGAACAGTCTATCCTCCTTTAG	144.91628
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93530_Hsan_Wil2021_COI	MD38	1	BB93530_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Absent	Sample	Hsan_Wil2021_COI	MD38	COI	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	CCTGGGCCGGTATAGTAGGT	144.91628
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93530_Msal_Bot2012_COI	MD38	1	BB93530_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Absent	Sample	Msal_Bot2012_COI	MD38	COI	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	GYTAGTTCCRATGATGTTAGCTG	144.91628
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.207	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93532_Aamu_Bax2006_COI	MD36	1	BB93532_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Present	Sample	Aamu_Bax2006_COI	MD36	COI	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	GCACAACCGGGATCTTTACTTC	144.90613
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93532_Pcan_Bot2011_IGS	MD36	1	BB93532_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD36	IGS	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	CGTAATCCTCAGTACTGGCTA	144.90613
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.832	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93532_Sspa_Oph2007_COI	MD36	1	BB93532_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Present	Sample	Sspa_Oph2007_COI	MD36	COI	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	GAAGGCCTTCCGATGATC	144.90613
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.808	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93532_Upin_Bot2011_COI	MD36	1	BB93532_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Present	Sample	Upin_Bot2011_COI	MD36	COI	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	CTTTAATTACAGCGTTTTTATTGTTGT	144.90613
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93533_Mgig_Bot2012_COI	MD33	1	BB93533_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Absent	Sample	Mgig_Bot2012_COI	MD33	COI	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	TCTTATTCGTTGGAGACTTTATAACCCT	144.91481
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93534_Cmae_Bot2011_COI	MD28	1	BB93534_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Absent	Sample	Cmae_Bot2011_COI	MD28	COI	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	ATGAACAGTCTATCCTCCTTTAG	144.9158
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93534_Hsan_Wil2021_COI	MD28	1	BB93534_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Absent	Sample	Hsan_Wil2021_COI	MD28	COI	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	CCTGGGCCGGTATAGTAGGT	144.9158
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93534_Msal_Bot2012_COI	MD28	1	BB93534_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Absent	Sample	Msal_Bot2012_COI	MD28	COI	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	GYTAGTTCCRATGATGTTAGCTG	144.9158
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.377	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93536_Aamu_Bax2006_COI	MD23	1	BB93536_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Present	Sample	Aamu_Bax2006_COI	MD23	COI	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	GCACAACCGGGATCTTTACTTC	144.90969
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93536_Pcan_Bot2011_IGS	MD23	1	BB93536_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD23	IGS	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	CGTAATCCTCAGTACTGGCTA	144.90969
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.728	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93536_Sspa_Oph2007_COI	MD23	1	BB93536_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Present	Sample	Sspa_Oph2007_COI	MD23	COI	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	GAAGGCCTTCCGATGATC	144.90969
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.911	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93536_Upin_Bot2011_COI	MD23	1	BB93536_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Present	Sample	Upin_Bot2011_COI	MD23	COI	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	CTTTAATTACAGCGTTTTTATTGTTGT	144.90969
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93537_Mgig_Bot2012_COI	MD21	1	BB93537_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Absent	Sample	Mgig_Bot2012_COI	MD21	COI	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	TCTTATTCGTTGGAGACTTTATAACCCT	144.90981
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93538_Cmae_Bot2011_COI	MD19	1	BB93538_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Absent	Sample	Cmae_Bot2011_COI	MD19	COI	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	ATGAACAGTCTATCCTCCTTTAG	144.90713
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93538_Hsan_Wil2021_COI	MD19	1	BB93538_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Absent	Sample	Hsan_Wil2021_COI	MD19	COI	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	CCTGGGCCGGTATAGTAGGT	144.90713
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93538_Msal_Bot2012_COI	MD19	1	BB93538_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Absent	Sample	Msal_Bot2012_COI	MD19	COI	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	GYTAGTTCCRATGATGTTAGCTG	144.90713
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93540_Mgig_Bot2012_COI	MD16	1	BB93540_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Absent	Sample	Mgig_Bot2012_COI	MD16	COI	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	TCTTATTCGTTGGAGACTTTATAACCCT	144.90716
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93541_Cmae_Bot2011_COI	MD14	1	BB93541_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Absent	Sample	Cmae_Bot2011_COI	MD14	COI	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	ATGAACAGTCTATCCTCCTTTAG	144.90545
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93541_Hsan_Wil2021_COI	MD14	1	BB93541_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Absent	Sample	Hsan_Wil2021_COI	MD14	COI	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	CCTGGGCCGGTATAGTAGGT	144.90545
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93541_Msal_Bot2012_COI	MD14	1	BB93541_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Absent	Sample	Msal_Bot2012_COI	MD14	COI	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	GYTAGTTCCRATGATGTTAGCTG	144.90545
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.655	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93543_Aamu_Bax2006_COI	MD11	1	BB93543_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Present	Sample	Aamu_Bax2006_COI	MD11	COI	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	GCACAACCGGGATCTTTACTTC	144.92611
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93543_Pcan_Bot2011_IGS	MD11	1	BB93543_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD11	IGS	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	CGTAATCCTCAGTACTGGCTA	144.92611
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.536	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93543_Sspa_Oph2007_COI	MD11	1	BB93543_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Present	Sample	Sspa_Oph2007_COI	MD11	COI	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	GAAGGCCTTCCGATGATC	144.92611
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.806	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93543_Upin_Bot2011_COI	MD11	1	BB93543_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Present	Sample	Upin_Bot2011_COI	MD11	COI	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	CTTTAATTACAGCGTTTTTATTGTTGT	144.92611
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93544_Mgig_Bot2012_COI	MD9	1	BB93544_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Absent	Sample	Mgig_Bot2012_COI	MD9	COI	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	TCTTATTCGTTGGAGACTTTATAACCCT	144.92807
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93545_Cmae_Bot2011_COI	MD7	1	BB93545_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Absent	Sample	Cmae_Bot2011_COI	MD7	COI	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	ATGAACAGTCTATCCTCCTTTAG	144.92959
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93545_Hsan_Wil2021_COI	MD7	1	BB93545_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Absent	Sample	Hsan_Wil2021_COI	MD7	COI	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	CCTGGGCCGGTATAGTAGGT	144.92959
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93545_Msal_Bot2012_COI	MD7	1	BB93545_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Absent	Sample	Msal_Bot2012_COI	MD7	COI	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	GYTAGTTCCRATGATGTTAGCTG	144.92959
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.386	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93547_Aamu_Bax2006_COI	MD4	1	BB93547_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Present	Sample	Aamu_Bax2006_COI	MD4	COI	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	GCACAACCGGGATCTTTACTTC	144.92859
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93547_Pcan_Bot2011_IGS	MD4	1	BB93547_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD4	IGS	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	CGTAATCCTCAGTACTGGCTA	144.92859
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.808	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93547_Sspa_Oph2007_COI	MD4	1	BB93547_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Present	Sample	Sspa_Oph2007_COI	MD4	COI	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	GAAGGCCTTCCGATGATC	144.92859
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.152	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93547_Upin_Bot2011_COI	MD4	1	BB93547_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Present	Sample	Upin_Bot2011_COI	MD4	COI	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	CTTTAATTACAGCGTTTTTATTGTTGT	144.92859
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93548_Mgig_Bot2012_COI	MD3	1	BB93548_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Absent	Sample	Mgig_Bot2012_COI	MD3	COI	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	TCTTATTCGTTGGAGACTTTATAACCCT	144.93105
BB93549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36339	WGS84	BB93549	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93549_Cmae_Bot2011_COI	BD60	1	BB93549_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93549	Sample	BB93549	targeted	Absent	Sample	Cmae_Bot2011_COI	BD60	COI	45	BB93549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD60	ATGAACAGTCTATCCTCCTTTAG	153.17593
BB93549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36339	WGS84	BB93549	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93549_Msal_Bot2012_COI	BD60	1	BB93549_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93549	Sample	BB93549	targeted	Absent	Sample	Msal_Bot2012_COI	BD60	COI	45	BB93549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD60	GYTAGTTCCRATGATGTTAGCTG	153.17593
BB93550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37147	WGS84	BB93550	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93550_Aamu_Bax2006_COI	BD55	1	BB93550_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93550	Sample	BB93550	targeted	Absent	Sample	Aamu_Bax2006_COI	BD55	COI	45	BB93550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD55	GCACAACCGGGATCTTTACTTC	153.1703
BB93550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37147	WGS84	BB93550	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93550_Pcan_Bot2011_IGS	BD55	1	BB93550_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93550	Sample	BB93550	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD55	IGS	45	BB93550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD55	CGTAATCCTCAGTACTGGCTA	153.1703
BB93550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37147	WGS84	BB93550	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93550_Sspa_Oph2007_COI	BD55	1	BB93550_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93550	Sample	BB93550	targeted	Absent	Sample	Sspa_Oph2007_COI	BD55	COI	45	BB93550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD55	GAAGGCCTTCCGATGATC	153.1703
BB93550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37147	WGS84	BB93550	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93550_Upin_Bot2011_COI	BD55	1	BB93550_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93550	Sample	BB93550	targeted	Absent	Sample	Upin_Bot2011_COI	BD55	COI	45	BB93550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD55	CTTTAATTACAGCGTTTTTATTGTTGT	153.1703
BB93551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37878	WGS84	BB93551	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93551_Mgig_Bot2012_COI	BD50	1	BB93551_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93551	Sample	BB93551	targeted	Absent	Sample	Mgig_Bot2012_COI	BD50	COI	45	BB93551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD50	TCTTATTCGTTGGAGACTTTATAACCCT	153.1647
BB93552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40307	WGS84	BB93552	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93552_Cmae_Bot2011_COI	BD35	1	BB93552_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93552	Sample	BB93552	targeted	Absent	Sample	Cmae_Bot2011_COI	BD35	COI	45	BB93552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD35	ATGAACAGTCTATCCTCCTTTAG	153.15355
BB93552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40307	WGS84	BB93552	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93552_Msal_Bot2012_COI	BD35	1	BB93552_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93552	Sample	BB93552	targeted	Absent	Sample	Msal_Bot2012_COI	BD35	COI	45	BB93552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD35	GYTAGTTCCRATGATGTTAGCTG	153.15355
BB93554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44768	WGS84	BB93554	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93554_Aamu_Bax2006_COI	BD15	1	BB93554_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93554	Sample	BB93554	targeted	Absent	Sample	Aamu_Bax2006_COI	BD15	COI	45	BB93554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD15	GCACAACCGGGATCTTTACTTC	153.0949
BB93554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44768	WGS84	BB93554	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93554_Pcan_Bot2011_IGS	BD15	1	BB93554_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93554	Sample	BB93554	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD15	IGS	45	BB93554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD15	CGTAATCCTCAGTACTGGCTA	153.0949
BB93554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44768	WGS84	BB93554	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93554_Sspa_Oph2007_COI	BD15	1	BB93554_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93554	Sample	BB93554	targeted	Absent	Sample	Sspa_Oph2007_COI	BD15	COI	45	BB93554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD15	GAAGGCCTTCCGATGATC	153.0949
BB93554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44768	WGS84	BB93554	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93554_Upin_Bot2011_COI	BD15	1	BB93554_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93554	Sample	BB93554	targeted	Absent	Sample	Upin_Bot2011_COI	BD15	COI	45	BB93554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD15	CTTTAATTACAGCGTTTTTATTGTTGT	153.0949
BB93555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3571	WGS84	BB93555	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93555_Mgig_Bot2012_COI	BD64	1	BB93555_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93555	Sample	BB93555	targeted	Absent	Sample	Mgig_Bot2012_COI	BD64	COI	45	BB93555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD64	TCTTATTCGTTGGAGACTTTATAACCCT	153.18065
BB93556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36005	WGS84	BB93556	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93556_Cmae_Bot2011_COI	BD62	1	BB93556_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93556	Sample	BB93556	targeted	Absent	Sample	Cmae_Bot2011_COI	BD62	COI	45	BB93556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD62	ATGAACAGTCTATCCTCCTTTAG	153.17846
BB93556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36005	WGS84	BB93556	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93556_Msal_Bot2012_COI	BD62	1	BB93556_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93556	Sample	BB93556	targeted	Absent	Sample	Msal_Bot2012_COI	BD62	COI	45	BB93556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD62	GYTAGTTCCRATGATGTTAGCTG	153.17846
BB93558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36857	WGS84	BB93558	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93558_Aamu_Bax2006_COI	BD57	1	BB93558_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93558	Sample	BB93558	targeted	Absent	Sample	Aamu_Bax2006_COI	BD57	COI	45	BB93558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD57	GCACAACCGGGATCTTTACTTC	153.17257
BB93558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36857	WGS84	BB93558	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93558_Pcan_Bot2011_IGS	BD57	1	BB93558_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93558	Sample	BB93558	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD57	IGS	45	BB93558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD57	CGTAATCCTCAGTACTGGCTA	153.17257
BB93558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36857	WGS84	BB93558	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93558_Sspa_Oph2007_COI	BD57	1	BB93558_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93558	Sample	BB93558	targeted	Absent	Sample	Sspa_Oph2007_COI	BD57	COI	45	BB93558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD57	GAAGGCCTTCCGATGATC	153.17257
BB93558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36857	WGS84	BB93558	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93558_Upin_Bot2011_COI	BD57	1	BB93558_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93558	Sample	BB93558	targeted	Absent	Sample	Upin_Bot2011_COI	BD57	COI	45	BB93558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD57	CTTTAATTACAGCGTTTTTATTGTTGT	153.17257
BB93559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37292	WGS84	BB93559	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93559_Mgig_Bot2012_COI	BD54	1	BB93559_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93559	Sample	BB93559	targeted	Absent	Sample	Mgig_Bot2012_COI	BD54	COI	45	BB93559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD54	TCTTATTCGTTGGAGACTTTATAACCCT	153.16914
BB93561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3773	WGS84	BB93561	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93561_Aamu_Bax2006_COI	BD51	1	BB93561_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93561	Sample	BB93561	targeted	Absent	Sample	Aamu_Bax2006_COI	BD51	COI	45	BB93561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD51	GCACAACCGGGATCTTTACTTC	153.16576
BB93561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3773	WGS84	BB93561	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93561_Pcan_Bot2011_IGS	BD51	1	BB93561_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93561	Sample	BB93561	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD51	IGS	45	BB93561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD51	CGTAATCCTCAGTACTGGCTA	153.16576
BB93561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3773	WGS84	BB93561	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93561_Sspa_Oph2007_COI	BD51	1	BB93561_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93561	Sample	BB93561	targeted	Absent	Sample	Sspa_Oph2007_COI	BD51	COI	45	BB93561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD51	GAAGGCCTTCCGATGATC	153.16576
BB93561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3773	WGS84	BB93561	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93561_Upin_Bot2011_COI	BD51	1	BB93561_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93561	Sample	BB93561	targeted	Absent	Sample	Upin_Bot2011_COI	BD51	COI	45	BB93561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD51	CTTTAATTACAGCGTTTTTATTGTTGT	153.16576
BB93562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38117	WGS84	BB93562	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93562_Mgig_Bot2012_COI	BD48	1	BB93562_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93562	Sample	BB93562	targeted	Absent	Sample	Mgig_Bot2012_COI	BD48	COI	45	BB93562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD48	TCTTATTCGTTGGAGACTTTATAACCCT	153.16243
BB93563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38398	WGS84	BB93563	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93563_Cmae_Bot2011_COI	BD46	1	BB93563_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93563	Sample	BB93563	targeted	Absent	Sample	Cmae_Bot2011_COI	BD46	COI	45	BB93563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD46	ATGAACAGTCTATCCTCCTTTAG	153.16056
BB93563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38398	WGS84	BB93563	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93563_Msal_Bot2012_COI	BD46	1	BB93563_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93563	Sample	BB93563	targeted	Absent	Sample	Msal_Bot2012_COI	BD46	COI	45	BB93563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD46	GYTAGTTCCRATGATGTTAGCTG	153.16056
BB93565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.39045	WGS84	BB93565	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93565_Aamu_Bax2006_COI	BD42	1	BB93565_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93565	Sample	BB93565	targeted	Absent	Sample	Aamu_Bax2006_COI	BD42	COI	45	BB93565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD42	GCACAACCGGGATCTTTACTTC	153.15746
BB93565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.39045	WGS84	BB93565	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93565_Pcan_Bot2011_IGS	BD42	1	BB93565_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93565	Sample	BB93565	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD42	IGS	45	BB93565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD42	CGTAATCCTCAGTACTGGCTA	153.15746
BB93565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.39045	WGS84	BB93565	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93565_Sspa_Oph2007_COI	BD42	1	BB93565_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93565	Sample	BB93565	targeted	Absent	Sample	Sspa_Oph2007_COI	BD42	COI	45	BB93565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD42	GAAGGCCTTCCGATGATC	153.15746
BB93565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.39045	WGS84	BB93565	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93565_Upin_Bot2011_COI	BD42	1	BB93565_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93565	Sample	BB93565	targeted	Absent	Sample	Upin_Bot2011_COI	BD42	COI	45	BB93565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD42	CTTTAATTACAGCGTTTTTATTGTTGT	153.15746
BB93566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40067	WGS84	BB93566	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93566_Mgig_Bot2012_COI	BD41	1	BB93566_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93566	Sample	BB93566	targeted	Absent	Sample	Mgig_Bot2012_COI	BD41	COI	45	BB93566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD41	TCTTATTCGTTGGAGACTTTATAACCCT	153.16346
BB93567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40091	WGS84	BB93567	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93567_Cmae_Bot2011_COI	BD39	1	BB93567_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93567	Sample	BB93567	targeted	Absent	Sample	Cmae_Bot2011_COI	BD39	COI	45	BB93567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD39	ATGAACAGTCTATCCTCCTTTAG	153.16222
BB93567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40091	WGS84	BB93567	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93567_Msal_Bot2012_COI	BD39	1	BB93567_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93567	Sample	BB93567	targeted	Absent	Sample	Msal_Bot2012_COI	BD39	COI	45	BB93567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD39	GYTAGTTCCRATGATGTTAGCTG	153.16222
BB93569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40516	WGS84	BB93569	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93569_Aamu_Bax2006_COI	BD33	1	BB93569_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93569	Sample	BB93569	targeted	Absent	Sample	Aamu_Bax2006_COI	BD33	COI	45	BB93569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD33	GCACAACCGGGATCTTTACTTC	153.15148
BB93569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40516	WGS84	BB93569	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93569_Pcan_Bot2011_IGS	BD33	1	BB93569_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93569	Sample	BB93569	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD33	IGS	45	BB93569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD33	CGTAATCCTCAGTACTGGCTA	153.15148
BB93569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40516	WGS84	BB93569	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93569_Sspa_Oph2007_COI	BD33	1	BB93569_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93569	Sample	BB93569	targeted	Absent	Sample	Sspa_Oph2007_COI	BD33	COI	45	BB93569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD33	GAAGGCCTTCCGATGATC	153.15148
BB93569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40516	WGS84	BB93569	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93569_Upin_Bot2011_COI	BD33	1	BB93569_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93569	Sample	BB93569	targeted	Absent	Sample	Upin_Bot2011_COI	BD33	COI	45	BB93569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD33	CTTTAATTACAGCGTTTTTATTGTTGT	153.15148
BB93570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.4123	WGS84	BB93570	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93570_Cmae_Bot2011_COI	BD32	1	BB93570_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93570	Sample	BB93570	targeted	Absent	Sample	Cmae_Bot2011_COI	BD32	COI	45	BB93570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD32	ATGAACAGTCTATCCTCCTTTAG	153.14395
BB93570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.4123	WGS84	BB93570	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93570_Msal_Bot2012_COI	BD32	1	BB93570_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93570	Sample	BB93570	targeted	Absent	Sample	Msal_Bot2012_COI	BD32	COI	45	BB93570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD32	GYTAGTTCCRATGATGTTAGCTG	153.14395
BB93572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42178	WGS84	BB93572	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93572_Aamu_Bax2006_COI	BD29	1	BB93572_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93572	Sample	BB93572	targeted	Absent	Sample	Aamu_Bax2006_COI	BD29	COI	45	BB93572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD29	GCACAACCGGGATCTTTACTTC	153.13521
BB93572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42178	WGS84	BB93572	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93572_Pcan_Bot2011_IGS	BD29	1	BB93572_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93572	Sample	BB93572	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD29	IGS	45	BB93572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD29	CGTAATCCTCAGTACTGGCTA	153.13521
BB93572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42178	WGS84	BB93572	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93572_Sspa_Oph2007_COI	BD29	1	BB93572_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93572	Sample	BB93572	targeted	Absent	Sample	Sspa_Oph2007_COI	BD29	COI	45	BB93572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD29	GAAGGCCTTCCGATGATC	153.13521
BB93572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42178	WGS84	BB93572	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93572_Upin_Bot2011_COI	BD29	1	BB93572_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93572	Sample	BB93572	targeted	Absent	Sample	Upin_Bot2011_COI	BD29	COI	45	BB93572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD29	CTTTAATTACAGCGTTTTTATTGTTGT	153.13521
BB93573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42503	WGS84	BB93573	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93573_Mgig_Bot2012_COI	BD27	1	BB93573_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93573	Sample	BB93573	targeted	Absent	Sample	Mgig_Bot2012_COI	BD27	COI	45	BB93573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD27	TCTTATTCGTTGGAGACTTTATAACCCT	153.12793
BB93574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.428	WGS84	BB93574	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93574_Cmae_Bot2011_COI	BD24	1	BB93574_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93574	Sample	BB93574	targeted	Absent	Sample	Cmae_Bot2011_COI	BD24	COI	45	BB93574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD24	ATGAACAGTCTATCCTCCTTTAG	153.12199
BB93574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.428	WGS84	BB93574	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93574_Msal_Bot2012_COI	BD24	1	BB93574_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93574	Sample	BB93574	targeted	Absent	Sample	Msal_Bot2012_COI	BD24	COI	45	BB93574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD24	GYTAGTTCCRATGATGTTAGCTG	153.12199
BB93576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43122	WGS84	BB93576	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93576_Aamu_Bax2006_COI	BD18	1	BB93576_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93576	Sample	BB93576	targeted	Absent	Sample	Aamu_Bax2006_COI	BD18	COI	45	BB93576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD18	GCACAACCGGGATCTTTACTTC	153.11783
BB93576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43122	WGS84	BB93576	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93576_Pcan_Bot2011_IGS	BD18	1	BB93576_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93576	Sample	BB93576	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD18	IGS	45	BB93576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD18	CGTAATCCTCAGTACTGGCTA	153.11783
BB93576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43122	WGS84	BB93576	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93576_Sspa_Oph2007_COI	BD18	1	BB93576_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93576	Sample	BB93576	targeted	Absent	Sample	Sspa_Oph2007_COI	BD18	COI	45	BB93576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD18	GAAGGCCTTCCGATGATC	153.11783
BB93576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43122	WGS84	BB93576	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93576_Upin_Bot2011_COI	BD18	1	BB93576_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93576	Sample	BB93576	targeted	Absent	Sample	Upin_Bot2011_COI	BD18	COI	45	BB93576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD18	CTTTAATTACAGCGTTTTTATTGTTGT	153.11783
BB93577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44736	WGS84	BB93577	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93577_Mgig_Bot2012_COI	BD16	1	BB93577_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93577	Sample	BB93577	targeted	Absent	Sample	Mgig_Bot2012_COI	BD16	COI	45	BB93577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD16	TCTTATTCGTTGGAGACTTTATAACCCT	153.09726
BB93578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44561	WGS84	BB93578	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93578_Cmae_Bot2011_COI	BD14	1	BB93578_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93578	Sample	BB93578	targeted	Absent	Sample	Cmae_Bot2011_COI	BD14	COI	45	BB93578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD14	ATGAACAGTCTATCCTCCTTTAG	153.08298
BB93578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44561	WGS84	BB93578	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93578_Msal_Bot2012_COI	BD14	1	BB93578_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93578	Sample	BB93578	targeted	Absent	Sample	Msal_Bot2012_COI	BD14	COI	45	BB93578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD14	GYTAGTTCCRATGATGTTAGCTG	153.08298
BB93580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44522	WGS84	BB93580	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93580_Mgig_Bot2012_COI	BD8	1	BB93580_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93580	Sample	BB93580	targeted	Absent	Sample	Mgig_Bot2012_COI	BD8	COI	45	BB93580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD8	TCTTATTCGTTGGAGACTTTATAACCCT	153.0791
BB93581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44533	WGS84	BB93581	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93581_Cmae_Bot2011_COI	BD7	1	BB93581_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93581	Sample	BB93581	targeted	Absent	Sample	Cmae_Bot2011_COI	BD7	COI	45	BB93581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD7	ATGAACAGTCTATCCTCCTTTAG	153.07768
BB93581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44533	WGS84	BB93581	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93581_Msal_Bot2012_COI	BD7	1	BB93581_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93581	Sample	BB93581	targeted	Absent	Sample	Msal_Bot2012_COI	BD7	COI	45	BB93581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD7	GYTAGTTCCRATGATGTTAGCTG	153.07768
BB93583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44569	WGS84	BB93583	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93583_Aamu_Bax2006_COI	BD4	1	BB93583_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93583	Sample	BB93583	targeted	Absent	Sample	Aamu_Bax2006_COI	BD4	COI	45	BB93583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD4	GCACAACCGGGATCTTTACTTC	153.07546
BB93583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44569	WGS84	BB93583	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93583_Pcan_Bot2011_IGS	BD4	1	BB93583_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93583	Sample	BB93583	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD4	IGS	45	BB93583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD4	CGTAATCCTCAGTACTGGCTA	153.07546
BB93583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44569	WGS84	BB93583	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93583_Sspa_Oph2007_COI	BD4	1	BB93583_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93583	Sample	BB93583	targeted	Absent	Sample	Sspa_Oph2007_COI	BD4	COI	45	BB93583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD4	GAAGGCCTTCCGATGATC	153.07546
BB93583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44569	WGS84	BB93583	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93583_Upin_Bot2011_COI	BD4	1	BB93583_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93583	Sample	BB93583	targeted	Absent	Sample	Upin_Bot2011_COI	BD4	COI	45	BB93583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD4	CTTTAATTACAGCGTTTTTATTGTTGT	153.07546
BB93584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44385	WGS84	BB93584	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93584_Mgig_Bot2012_COI	BD3	1	BB93584_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93584	Sample	BB93584	targeted	Absent	Sample	Mgig_Bot2012_COI	BD3	COI	45	BB93584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD3	TCTTATTCGTTGGAGACTTTATAACCCT	153.07508
BB93585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44293	WGS84	BB93585	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93585_Cmae_Bot2011_COI	BD2	1	BB93585_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93585	Sample	BB93585	targeted	Absent	Sample	Cmae_Bot2011_COI	BD2	COI	45	BB93585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD2	ATGAACAGTCTATCCTCCTTTAG	153.07323
BB93585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44293	WGS84	BB93585	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93585_Msal_Bot2012_COI	BD2	1	BB93585_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93585	Sample	BB93585	targeted	Absent	Sample	Msal_Bot2012_COI	BD2	COI	45	BB93585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD2	GYTAGTTCCRATGATGTTAGCTG	153.07323
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93587_Aamu_Bax2006_COI	YD138	1	BB93587_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Aamu_Bax2006_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	GCACAACCGGGATCTTTACTTC	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93587_Pcan_Bot2011_IGS	YD138	1	BB93587_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD138	IGS	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	CGTAATCCTCAGTACTGGCTA	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93587_Pper_Dia2013_COI	YD138	1	BB93587_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Pper_Dia2013_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	CTTAGTGGCATTAATTCGDAATCC	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93587_Sspa_Oph2007_COI	YD138	1	BB93587_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Sspa_Oph2007_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	GAAGGCCTTCCGATGATC	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93587_Upin_Bot2011_COI	YD138	1	BB93587_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Upin_Bot2011_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	CTTTAATTACAGCGTTTTTATTGTTGT	149.31354
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93588_Cjap_Sim2018_COI	YD136	1	BB93588_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Cjap_Sim2018_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93588_Esin_And2018_Cyb	YD136	1	BB93588_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Esin_And2018_Cyb	YD136	Cytb	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	ACCCCTCCTCATATCCAACCA	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93588_Mgig_Bot2012_COI	YD136	1	BB93588_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Mgig_Bot2012_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	TCTTATTCGTTGGAGACTTTATAACCCT	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93588_Mjap_Gib2022_COI	YD136	1	BB93588_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Mjap_Gib2022_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	AATTTAATTGTGACTAGGCATGGTCTG	149.31324
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93589_Cmae_Bot2011_COI	YD134	1	BB93589_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Cmae_Bot2011_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	ATGAACAGTCTATCCTCCTTTAG	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93589_Hsan_Wil2021_COI	YD134	1	BB93589_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Hsan_Wil2021_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	CCTGGGCCGGTATAGTAGGT	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93589_Mros_Gib2022_COI	YD134	1	BB93589_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Mros_Gib2022_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93589_Msal_Bot2012_COI	YD134	1	BB93589_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Msal_Bot2012_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	GYTAGTTCCRATGATGTTAGCTG	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93589_Pvir_Dia2013_COI	YD134	1	BB93589_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Pvir_Dia2013_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	CTTAGTGGCATTAATTCGDAATCC	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93589_Rhar_Sim2018_COI	YD134	1	BB93589_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Rhar_Sim2018_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	CTGCTGCTATTGCTCATG	149.31226
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93590_Aamu_Bax2006_COI	YD132	1	BB93590_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Aamu_Bax2006_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	GCACAACCGGGATCTTTACTTC	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93590_Pcan_Bot2011_IGS	YD132	1	BB93590_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD132	IGS	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	CGTAATCCTCAGTACTGGCTA	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93590_Pper_Dia2013_COI	YD132	1	BB93590_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Pper_Dia2013_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	CTTAGTGGCATTAATTCGDAATCC	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93590_Sspa_Oph2007_COI	YD132	1	BB93590_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Sspa_Oph2007_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	GAAGGCCTTCCGATGATC	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93590_Upin_Bot2011_COI	YD132	1	BB93590_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Upin_Bot2011_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	CTTTAATTACAGCGTTTTTATTGTTGT	149.31331
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93591_Cjap_Sim2018_COI	YD131	1	BB93591_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Cjap_Sim2018_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93591_Esin_And2018_Cyb	YD131	1	BB93591_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Esin_And2018_Cyb	YD131	Cytb	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	ACCCCTCCTCATATCCAACCA	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93591_Mgig_Bot2012_COI	YD131	1	BB93591_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Mgig_Bot2012_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	TCTTATTCGTTGGAGACTTTATAACCCT	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93591_Mjap_Gib2022_COI	YD131	1	BB93591_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Mjap_Gib2022_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	AATTTAATTGTGACTAGGCATGGTCTG	149.3143
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93592_Cmae_Bot2011_COI	YD129	1	BB93592_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Cmae_Bot2011_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	ATGAACAGTCTATCCTCCTTTAG	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93592_Hsan_Wil2021_COI	YD129	1	BB93592_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Hsan_Wil2021_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	CCTGGGCCGGTATAGTAGGT	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93592_Mros_Gib2022_COI	YD129	1	BB93592_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Mros_Gib2022_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93592_Msal_Bot2012_COI	YD129	1	BB93592_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Msal_Bot2012_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	GYTAGTTCCRATGATGTTAGCTG	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93592_Pvir_Dia2013_COI	YD129	1	BB93592_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Pvir_Dia2013_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	CTTAGTGGCATTAATTCGDAATCC	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93592_Rhar_Sim2018_COI	YD129	1	BB93592_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Rhar_Sim2018_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	CTGCTGCTATTGCTCATG	149.31412
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93594_Aamu_Bax2006_COI	YD127	1	BB93594_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Aamu_Bax2006_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	GCACAACCGGGATCTTTACTTC	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93594_Pcan_Bot2011_IGS	YD127	1	BB93594_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD127	IGS	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	CGTAATCCTCAGTACTGGCTA	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93594_Pper_Dia2013_COI	YD127	1	BB93594_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Pper_Dia2013_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	CTTAGTGGCATTAATTCGDAATCC	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93594_Sspa_Oph2007_COI	YD127	1	BB93594_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Sspa_Oph2007_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	GAAGGCCTTCCGATGATC	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93594_Upin_Bot2011_COI	YD127	1	BB93594_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Upin_Bot2011_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	CTTTAATTACAGCGTTTTTATTGTTGT	149.31381
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93595_Cjap_Sim2018_COI	YD124	1	BB93595_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Cjap_Sim2018_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93595_Esin_And2018_Cyb	YD124	1	BB93595_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Esin_And2018_Cyb	YD124	Cytb	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	ACCCCTCCTCATATCCAACCA	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93595_Mgig_Bot2012_COI	YD124	1	BB93595_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Mgig_Bot2012_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	TCTTATTCGTTGGAGACTTTATAACCCT	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93595_Mjap_Gib2022_COI	YD124	1	BB93595_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Mjap_Gib2022_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	AATTTAATTGTGACTAGGCATGGTCTG	149.31554
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93596_Cmae_Bot2011_COI	YD123	1	BB93596_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Cmae_Bot2011_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	ATGAACAGTCTATCCTCCTTTAG	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93596_Hsan_Wil2021_COI	YD123	1	BB93596_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Hsan_Wil2021_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	CCTGGGCCGGTATAGTAGGT	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93596_Mros_Gib2022_COI	YD123	1	BB93596_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Mros_Gib2022_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93596_Msal_Bot2012_COI	YD123	1	BB93596_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Msal_Bot2012_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	GYTAGTTCCRATGATGTTAGCTG	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93596_Pvir_Dia2013_COI	YD123	1	BB93596_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Pvir_Dia2013_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	CTTAGTGGCATTAATTCGDAATCC	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93596_Rhar_Sim2018_COI	YD123	1	BB93596_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Rhar_Sim2018_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	CTGCTGCTATTGCTCATG	149.31475
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93598_Aamu_Bax2006_COI	YD118	1	BB93598_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Aamu_Bax2006_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	GCACAACCGGGATCTTTACTTC	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93598_Pcan_Bot2011_IGS	YD118	1	BB93598_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD118	IGS	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	CGTAATCCTCAGTACTGGCTA	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93598_Pper_Dia2013_COI	YD118	1	BB93598_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Pper_Dia2013_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	CTTAGTGGCATTAATTCGDAATCC	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93598_Sspa_Oph2007_COI	YD118	1	BB93598_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Sspa_Oph2007_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	GAAGGCCTTCCGATGATC	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93598_Upin_Bot2011_COI	YD118	1	BB93598_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Upin_Bot2011_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	CTTTAATTACAGCGTTTTTATTGTTGT	149.31427
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93599_Cjap_Sim2018_COI	YD117	1	BB93599_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Cjap_Sim2018_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93599_Esin_And2018_Cyb	YD117	1	BB93599_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Esin_And2018_Cyb	YD117	Cytb	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	ACCCCTCCTCATATCCAACCA	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93599_Mgig_Bot2012_COI	YD117	1	BB93599_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Mgig_Bot2012_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	TCTTATTCGTTGGAGACTTTATAACCCT	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93599_Mjap_Gib2022_COI	YD117	1	BB93599_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Mjap_Gib2022_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	AATTTAATTGTGACTAGGCATGGTCTG	149.31395
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93600_Cmae_Bot2011_COI	YD116	1	BB93600_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Cmae_Bot2011_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	ATGAACAGTCTATCCTCCTTTAG	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93600_Hsan_Wil2021_COI	YD116	1	BB93600_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Hsan_Wil2021_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	CCTGGGCCGGTATAGTAGGT	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93600_Mros_Gib2022_COI	YD116	1	BB93600_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Mros_Gib2022_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93600_Msal_Bot2012_COI	YD116	1	BB93600_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Msal_Bot2012_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	GYTAGTTCCRATGATGTTAGCTG	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93600_Pvir_Dia2013_COI	YD116	1	BB93600_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Pvir_Dia2013_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	CTTAGTGGCATTAATTCGDAATCC	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93600_Rhar_Sim2018_COI	YD116	1	BB93600_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Rhar_Sim2018_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	CTGCTGCTATTGCTCATG	149.31627
BB93602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26621	WGS84	BB93602	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93602_Aamu_Bax2006_COI	YD113	1	BB93602_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93602	Sample	BB93602	targeted	Absent	Sample	Aamu_Bax2006_COI	YD113	COI	45	BB93602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD113	GCACAACCGGGATCTTTACTTC	149.31567
BB93602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26621	WGS84	BB93602	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93602_Pcan_Bot2011_IGS	YD113	1	BB93602_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93602	Sample	BB93602	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD113	IGS	45	BB93602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD113	CGTAATCCTCAGTACTGGCTA	149.31567
BB93602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26621	WGS84	BB93602	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93602_Sspa_Oph2007_COI	YD113	1	BB93602_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93602	Sample	BB93602	targeted	Absent	Sample	Sspa_Oph2007_COI	YD113	COI	45	BB93602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD113	GAAGGCCTTCCGATGATC	149.31567
BB93602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26621	WGS84	BB93602	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93602_Upin_Bot2011_COI	YD113	1	BB93602_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93602	Sample	BB93602	targeted	Absent	Sample	Upin_Bot2011_COI	YD113	COI	45	BB93602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD113	CTTTAATTACAGCGTTTTTATTGTTGT	149.31567
BB93603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25606	WGS84	BB93603	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93603_Mgig_Bot2012_COI	YD110	1	BB93603_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93603	Sample	BB93603	targeted	Absent	Sample	Mgig_Bot2012_COI	YD110	COI	45	BB93603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD110	TCTTATTCGTTGGAGACTTTATAACCCT	149.30718
BB93604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25593	WGS84	BB93604	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93604_Cmae_Bot2011_COI	YD108	1	BB93604_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93604	Sample	BB93604	targeted	Absent	Sample	Cmae_Bot2011_COI	YD108	COI	45	BB93604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD108	ATGAACAGTCTATCCTCCTTTAG	149.30946
BB93604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25593	WGS84	BB93604	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93604_Msal_Bot2012_COI	YD108	1	BB93604_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93604	Sample	BB93604	targeted	Absent	Sample	Msal_Bot2012_COI	YD108	COI	45	BB93604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD108	GYTAGTTCCRATGATGTTAGCTG	149.30946
BB93606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26754	WGS84	BB93606	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93606_Aamu_Bax2006_COI	YD106	1	BB93606_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93606	Sample	BB93606	targeted	Absent	Sample	Aamu_Bax2006_COI	YD106	COI	45	BB93606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD106	GCACAACCGGGATCTTTACTTC	149.31291
BB93606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26754	WGS84	BB93606	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93606_Pcan_Bot2011_IGS	YD106	1	BB93606_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93606	Sample	BB93606	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD106	IGS	45	BB93606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD106	CGTAATCCTCAGTACTGGCTA	149.31291
BB93606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26754	WGS84	BB93606	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93606_Sspa_Oph2007_COI	YD106	1	BB93606_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93606	Sample	BB93606	targeted	Absent	Sample	Sspa_Oph2007_COI	YD106	COI	45	BB93606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD106	GAAGGCCTTCCGATGATC	149.31291
BB93606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26754	WGS84	BB93606	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93606_Upin_Bot2011_COI	YD106	1	BB93606_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93606	Sample	BB93606	targeted	Absent	Sample	Upin_Bot2011_COI	YD106	COI	45	BB93606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD106	CTTTAATTACAGCGTTTTTATTGTTGT	149.31291
BB93607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26817	WGS84	BB93607	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93607_Mgig_Bot2012_COI	YD105	1	BB93607_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93607	Sample	BB93607	targeted	Absent	Sample	Mgig_Bot2012_COI	YD105	COI	45	BB93607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD105	TCTTATTCGTTGGAGACTTTATAACCCT	149.31344
BB93608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25174	WGS84	BB93608	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93608_Cmae_Bot2011_COI	YD103	1	BB93608_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93608	Sample	BB93608	targeted	Absent	Sample	Cmae_Bot2011_COI	YD103	COI	45	BB93608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD103	ATGAACAGTCTATCCTCCTTTAG	149.30413
BB93608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25174	WGS84	BB93608	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93608_Msal_Bot2012_COI	YD103	1	BB93608_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93608	Sample	BB93608	targeted	Absent	Sample	Msal_Bot2012_COI	YD103	COI	45	BB93608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD103	GYTAGTTCCRATGATGTTAGCTG	149.30413
BB93610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25367	WGS84	BB93610	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93610_Mgig_Bot2012_COI	YD99	1	BB93610_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93610	Sample	BB93610	targeted	Absent	Sample	Mgig_Bot2012_COI	YD99	COI	45	BB93610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD99	TCTTATTCGTTGGAGACTTTATAACCCT	149.30558
BB93611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25356	WGS84	BB93611	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93611_Cmae_Bot2011_COI	YD98	1	BB93611_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93611	Sample	BB93611	targeted	Absent	Sample	Cmae_Bot2011_COI	YD98	COI	45	BB93611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD98	ATGAACAGTCTATCCTCCTTTAG	149.30649
BB93611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25356	WGS84	BB93611	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93611_Msal_Bot2012_COI	YD98	1	BB93611_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93611	Sample	BB93611	targeted	Absent	Sample	Msal_Bot2012_COI	YD98	COI	45	BB93611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD98	GYTAGTTCCRATGATGTTAGCTG	149.30649
BB93613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25424	WGS84	BB93613	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93613_Aamu_Bax2006_COI	YD96	1	BB93613_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93613	Sample	BB93613	targeted	Absent	Sample	Aamu_Bax2006_COI	YD96	COI	45	BB93613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD96	GCACAACCGGGATCTTTACTTC	149.30705
BB93613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25424	WGS84	BB93613	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93613_Pcan_Bot2011_IGS	YD96	1	BB93613_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93613	Sample	BB93613	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD96	IGS	45	BB93613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD96	CGTAATCCTCAGTACTGGCTA	149.30705
BB93613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25424	WGS84	BB93613	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93613_Sspa_Oph2007_COI	YD96	1	BB93613_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93613	Sample	BB93613	targeted	Absent	Sample	Sspa_Oph2007_COI	YD96	COI	45	BB93613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD96	GAAGGCCTTCCGATGATC	149.30705
BB93613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25424	WGS84	BB93613	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93613_Upin_Bot2011_COI	YD96	1	BB93613_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93613	Sample	BB93613	targeted	Absent	Sample	Upin_Bot2011_COI	YD96	COI	45	BB93613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD96	CTTTAATTACAGCGTTTTTATTGTTGT	149.30705
BB93614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25563	WGS84	BB93614	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93614_Mgig_Bot2012_COI	YD94	1	BB93614_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93614	Sample	BB93614	targeted	Absent	Sample	Mgig_Bot2012_COI	YD94	COI	45	BB93614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD94	TCTTATTCGTTGGAGACTTTATAACCCT	149.30793
BB93615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25704	WGS84	BB93615	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93615_Cmae_Bot2011_COI	YD92	1	BB93615_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93615	Sample	BB93615	targeted	Absent	Sample	Cmae_Bot2011_COI	YD92	COI	45	BB93615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD92	ATGAACAGTCTATCCTCCTTTAG	149.30826
BB93615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25704	WGS84	BB93615	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93615_Msal_Bot2012_COI	YD92	1	BB93615_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93615	Sample	BB93615	targeted	Absent	Sample	Msal_Bot2012_COI	YD92	COI	45	BB93615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD92	GYTAGTTCCRATGATGTTAGCTG	149.30826
BB93617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25837	WGS84	BB93617	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93617_Aamu_Bax2006_COI	YD89	1	BB93617_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93617	Sample	BB93617	targeted	Absent	Sample	Aamu_Bax2006_COI	YD89	COI	45	BB93617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD89	GCACAACCGGGATCTTTACTTC	149.30937
BB93617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25837	WGS84	BB93617	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93617_Pcan_Bot2011_IGS	YD89	1	BB93617_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93617	Sample	BB93617	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD89	IGS	45	BB93617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD89	CGTAATCCTCAGTACTGGCTA	149.30937
BB93617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25837	WGS84	BB93617	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93617_Sspa_Oph2007_COI	YD89	1	BB93617_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93617	Sample	BB93617	targeted	Absent	Sample	Sspa_Oph2007_COI	YD89	COI	45	BB93617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD89	GAAGGCCTTCCGATGATC	149.30937
BB93617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25837	WGS84	BB93617	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93617_Upin_Bot2011_COI	YD89	1	BB93617_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93617	Sample	BB93617	targeted	Absent	Sample	Upin_Bot2011_COI	YD89	COI	45	BB93617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD89	CTTTAATTACAGCGTTTTTATTGTTGT	149.30937
BB93618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2592	WGS84	BB93618	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93618_Mgig_Bot2012_COI	YD88	1	BB93618_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93618	Sample	BB93618	targeted	Absent	Sample	Mgig_Bot2012_COI	YD88	COI	45	BB93618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD88	TCTTATTCGTTGGAGACTTTATAACCCT	149.30901
BB93619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26054	WGS84	BB93619	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93619_Cmae_Bot2011_COI	YD85	1	BB93619_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93619	Sample	BB93619	targeted	Absent	Sample	Cmae_Bot2011_COI	YD85	COI	45	BB93619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD85	ATGAACAGTCTATCCTCCTTTAG	149.31013
BB93619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26054	WGS84	BB93619	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93619_Msal_Bot2012_COI	YD85	1	BB93619_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93619	Sample	BB93619	targeted	Absent	Sample	Msal_Bot2012_COI	YD85	COI	45	BB93619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD85	GYTAGTTCCRATGATGTTAGCTG	149.31013
BB93620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25977	WGS84	BB93620	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93620_Aamu_Bax2006_COI	YD84	1	BB93620_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93620	Sample	BB93620	targeted	Absent	Sample	Aamu_Bax2006_COI	YD84	COI	45	BB93620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD84	GCACAACCGGGATCTTTACTTC	149.31052
BB93620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25977	WGS84	BB93620	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93620_Pcan_Bot2011_IGS	YD84	1	BB93620_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93620	Sample	BB93620	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD84	IGS	45	BB93620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD84	CGTAATCCTCAGTACTGGCTA	149.31052
BB93620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25977	WGS84	BB93620	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93620_Sspa_Oph2007_COI	YD84	1	BB93620_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93620	Sample	BB93620	targeted	Absent	Sample	Sspa_Oph2007_COI	YD84	COI	45	BB93620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD84	GAAGGCCTTCCGATGATC	149.31052
BB93620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25977	WGS84	BB93620	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93620_Upin_Bot2011_COI	YD84	1	BB93620_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93620	Sample	BB93620	targeted	Absent	Sample	Upin_Bot2011_COI	YD84	COI	45	BB93620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD84	CTTTAATTACAGCGTTTTTATTGTTGT	149.31052
BB93621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26044	WGS84	BB93621	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93621_Mgig_Bot2012_COI	YD83	1	BB93621_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93621	Sample	BB93621	targeted	Absent	Sample	Mgig_Bot2012_COI	YD83	COI	45	BB93621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD83	TCTTATTCGTTGGAGACTTTATAACCCT	149.31104
BB93622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26411	WGS84	BB93622	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93622_Cmae_Bot2011_COI	YD81	1	BB93622_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93622	Sample	BB93622	targeted	Absent	Sample	Cmae_Bot2011_COI	YD81	COI	45	BB93622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD81	ATGAACAGTCTATCCTCCTTTAG	149.31103
BB93622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26411	WGS84	BB93622	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93622_Msal_Bot2012_COI	YD81	1	BB93622_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93622	Sample	BB93622	targeted	Absent	Sample	Msal_Bot2012_COI	YD81	COI	45	BB93622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD81	GYTAGTTCCRATGATGTTAGCTG	149.31103
BB93624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26709	WGS84	BB93624	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93624_Aamu_Bax2006_COI	YD75	1	BB93624_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93624	Sample	BB93624	targeted	Absent	Sample	Aamu_Bax2006_COI	YD75	COI	45	BB93624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD75	GCACAACCGGGATCTTTACTTC	149.31643
BB93624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26709	WGS84	BB93624	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93624_Pcan_Bot2011_IGS	YD75	1	BB93624_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93624	Sample	BB93624	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD75	IGS	45	BB93624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD75	CGTAATCCTCAGTACTGGCTA	149.31643
BB93624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26709	WGS84	BB93624	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93624_Sspa_Oph2007_COI	YD75	1	BB93624_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93624	Sample	BB93624	targeted	Absent	Sample	Sspa_Oph2007_COI	YD75	COI	45	BB93624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD75	GAAGGCCTTCCGATGATC	149.31643
BB93624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26709	WGS84	BB93624	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93624_Upin_Bot2011_COI	YD75	1	BB93624_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93624	Sample	BB93624	targeted	Absent	Sample	Upin_Bot2011_COI	YD75	COI	45	BB93624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD75	CTTTAATTACAGCGTTTTTATTGTTGT	149.31643
BB93625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26792	WGS84	BB93625	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93625_Mgig_Bot2012_COI	YD74	1	BB93625_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93625	Sample	BB93625	targeted	Absent	Sample	Mgig_Bot2012_COI	YD74	COI	45	BB93625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD74	TCTTATTCGTTGGAGACTTTATAACCCT	149.31616
BB93626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2573	WGS84	BB93626	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93626_Cmae_Bot2011_COI	YD73	1	BB93626_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93626	Sample	BB93626	targeted	Absent	Sample	Cmae_Bot2011_COI	YD73	COI	45	BB93626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD73	ATGAACAGTCTATCCTCCTTTAG	149.31246
BB93626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2573	WGS84	BB93626	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93626_Msal_Bot2012_COI	YD73	1	BB93626_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93626	Sample	BB93626	targeted	Absent	Sample	Msal_Bot2012_COI	YD73	COI	45	BB93626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD73	GYTAGTTCCRATGATGTTAGCTG	149.31246
BB93628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25665	WGS84	BB93628	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93628_Aamu_Bax2006_COI	YD70	1	BB93628_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93628	Sample	BB93628	targeted	Absent	Sample	Aamu_Bax2006_COI	YD70	COI	45	BB93628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD70	GCACAACCGGGATCTTTACTTC	149.31193
BB93628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25665	WGS84	BB93628	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93628_Pcan_Bot2011_IGS	YD70	1	BB93628_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93628	Sample	BB93628	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD70	IGS	45	BB93628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD70	CGTAATCCTCAGTACTGGCTA	149.31193
BB93628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25665	WGS84	BB93628	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93628_Sspa_Oph2007_COI	YD70	1	BB93628_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93628	Sample	BB93628	targeted	Absent	Sample	Sspa_Oph2007_COI	YD70	COI	45	BB93628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD70	GAAGGCCTTCCGATGATC	149.31193
BB93628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25665	WGS84	BB93628	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93628_Upin_Bot2011_COI	YD70	1	BB93628_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93628	Sample	BB93628	targeted	Absent	Sample	Upin_Bot2011_COI	YD70	COI	45	BB93628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD70	CTTTAATTACAGCGTTTTTATTGTTGT	149.31193
BB93629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25774	WGS84	BB93629	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93629_Mgig_Bot2012_COI	YD69	1	BB93629_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93629	Sample	BB93629	targeted	Absent	Sample	Mgig_Bot2012_COI	YD69	COI	45	BB93629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD69	TCTTATTCGTTGGAGACTTTATAACCCT	149.31154
BB93631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25608	WGS84	BB93631	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93631_Aamu_Bax2006_COI	YD64	1	BB93631_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93631	Sample	BB93631	targeted	Absent	Sample	Aamu_Bax2006_COI	YD64	COI	45	BB93631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD64	GCACAACCGGGATCTTTACTTC	149.31047
BB93631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25608	WGS84	BB93631	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93631_Pcan_Bot2011_IGS	YD64	1	BB93631_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93631	Sample	BB93631	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD64	IGS	45	BB93631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD64	CGTAATCCTCAGTACTGGCTA	149.31047
BB93631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25608	WGS84	BB93631	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93631_Sspa_Oph2007_COI	YD64	1	BB93631_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93631	Sample	BB93631	targeted	Absent	Sample	Sspa_Oph2007_COI	YD64	COI	45	BB93631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD64	GAAGGCCTTCCGATGATC	149.31047
BB93631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25608	WGS84	BB93631	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93631_Upin_Bot2011_COI	YD64	1	BB93631_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93631	Sample	BB93631	targeted	Absent	Sample	Upin_Bot2011_COI	YD64	COI	45	BB93631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD64	CTTTAATTACAGCGTTTTTATTGTTGT	149.31047
BB93632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25453	WGS84	BB93632	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93632_Mgig_Bot2012_COI	YD63	1	BB93632_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93632	Sample	BB93632	targeted	Absent	Sample	Mgig_Bot2012_COI	YD63	COI	45	BB93632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD63	TCTTATTCGTTGGAGACTTTATAACCCT	149.31127
BB93633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25462	WGS84	BB93633	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93633_Cmae_Bot2011_COI	YD62	1	BB93633_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93633	Sample	BB93633	targeted	Absent	Sample	Cmae_Bot2011_COI	YD62	COI	45	BB93633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD62	ATGAACAGTCTATCCTCCTTTAG	149.3103
BB93633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25462	WGS84	BB93633	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93633_Msal_Bot2012_COI	YD62	1	BB93633_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93633	Sample	BB93633	targeted	Absent	Sample	Msal_Bot2012_COI	YD62	COI	45	BB93633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD62	GYTAGTTCCRATGATGTTAGCTG	149.3103
BB93635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25324	WGS84	BB93635	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93635_Aamu_Bax2006_COI	YD55	1	BB93635_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93635	Sample	BB93635	targeted	Absent	Sample	Aamu_Bax2006_COI	YD55	COI	45	BB93635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD55	GCACAACCGGGATCTTTACTTC	149.30899
BB93635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25324	WGS84	BB93635	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93635_Pcan_Bot2011_IGS	YD55	1	BB93635_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93635	Sample	BB93635	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD55	IGS	45	BB93635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD55	CGTAATCCTCAGTACTGGCTA	149.30899
BB93635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25324	WGS84	BB93635	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93635_Sspa_Oph2007_COI	YD55	1	BB93635_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93635	Sample	BB93635	targeted	Absent	Sample	Sspa_Oph2007_COI	YD55	COI	45	BB93635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD55	GAAGGCCTTCCGATGATC	149.30899
BB93635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25324	WGS84	BB93635	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93635_Upin_Bot2011_COI	YD55	1	BB93635_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93635	Sample	BB93635	targeted	Absent	Sample	Upin_Bot2011_COI	YD55	COI	45	BB93635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD55	CTTTAATTACAGCGTTTTTATTGTTGT	149.30899
BB93636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25376	WGS84	BB93636	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93636_Mgig_Bot2012_COI	YD54	1	BB93636_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93636	Sample	BB93636	targeted	Absent	Sample	Mgig_Bot2012_COI	YD54	COI	45	BB93636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD54	TCTTATTCGTTGGAGACTTTATAACCCT	149.30868
BB93637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25107	WGS84	BB93637	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93637_Cmae_Bot2011_COI	YD52	1	BB93637_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93637	Sample	BB93637	targeted	Absent	Sample	Cmae_Bot2011_COI	YD52	COI	45	BB93637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD52	ATGAACAGTCTATCCTCCTTTAG	149.30902
BB93637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25107	WGS84	BB93637	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93637_Msal_Bot2012_COI	YD52	1	BB93637_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93637	Sample	BB93637	targeted	Absent	Sample	Msal_Bot2012_COI	YD52	COI	45	BB93637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD52	GYTAGTTCCRATGATGTTAGCTG	149.30902
BB93639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24849	WGS84	BB93639	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93639_Aamu_Bax2006_COI	YD47	1	BB93639_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93639	Sample	BB93639	targeted	Absent	Sample	Aamu_Bax2006_COI	YD47	COI	45	BB93639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD47	GCACAACCGGGATCTTTACTTC	149.30523
BB93639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24849	WGS84	BB93639	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93639_Pcan_Bot2011_IGS	YD47	1	BB93639_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93639	Sample	BB93639	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD47	IGS	45	BB93639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD47	CGTAATCCTCAGTACTGGCTA	149.30523
BB93639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24849	WGS84	BB93639	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93639_Sspa_Oph2007_COI	YD47	1	BB93639_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93639	Sample	BB93639	targeted	Absent	Sample	Sspa_Oph2007_COI	YD47	COI	45	BB93639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD47	GAAGGCCTTCCGATGATC	149.30523
BB93639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24849	WGS84	BB93639	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93639_Upin_Bot2011_COI	YD47	1	BB93639_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93639	Sample	BB93639	targeted	Absent	Sample	Upin_Bot2011_COI	YD47	COI	45	BB93639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD47	CTTTAATTACAGCGTTTTTATTGTTGT	149.30523
BB93640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24547	WGS84	BB93640	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93640_Cmae_Bot2011_COI	YD46	1	BB93640_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93640	Sample	BB93640	targeted	Absent	Sample	Cmae_Bot2011_COI	YD46	COI	45	BB93640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD46	ATGAACAGTCTATCCTCCTTTAG	149.30575
BB93640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24547	WGS84	BB93640	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93640_Msal_Bot2012_COI	YD46	1	BB93640_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93640	Sample	BB93640	targeted	Absent	Sample	Msal_Bot2012_COI	YD46	COI	45	BB93640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD46	GYTAGTTCCRATGATGTTAGCTG	149.30575
BB93642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2454	WGS84	BB93642	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93642_Aamu_Bax2006_COI	YD42	1	BB93642_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93642	Sample	BB93642	targeted	Absent	Sample	Aamu_Bax2006_COI	YD42	COI	45	BB93642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD42	GCACAACCGGGATCTTTACTTC	149.30471
BB93642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2454	WGS84	BB93642	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93642_Pcan_Bot2011_IGS	YD42	1	BB93642_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93642	Sample	BB93642	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD42	IGS	45	BB93642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD42	CGTAATCCTCAGTACTGGCTA	149.30471
BB93642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2454	WGS84	BB93642	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93642_Sspa_Oph2007_COI	YD42	1	BB93642_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93642	Sample	BB93642	targeted	Absent	Sample	Sspa_Oph2007_COI	YD42	COI	45	BB93642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD42	GAAGGCCTTCCGATGATC	149.30471
BB93642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2454	WGS84	BB93642	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93642_Upin_Bot2011_COI	YD42	1	BB93642_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93642	Sample	BB93642	targeted	Absent	Sample	Upin_Bot2011_COI	YD42	COI	45	BB93642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD42	CTTTAATTACAGCGTTTTTATTGTTGT	149.30471
BB93643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24635	WGS84	BB93643	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93643_Mgig_Bot2012_COI	YD41	1	BB93643_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93643	Sample	BB93643	targeted	Absent	Sample	Mgig_Bot2012_COI	YD41	COI	45	BB93643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD41	TCTTATTCGTTGGAGACTTTATAACCCT	149.30463
BB93644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24415	WGS84	BB93644	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93644_Cmae_Bot2011_COI	YD40	1	BB93644_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93644	Sample	BB93644	targeted	Absent	Sample	Cmae_Bot2011_COI	YD40	COI	45	BB93644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD40	ATGAACAGTCTATCCTCCTTTAG	149.30462
BB93644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24415	WGS84	BB93644	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93644_Msal_Bot2012_COI	YD40	1	BB93644_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93644	Sample	BB93644	targeted	Absent	Sample	Msal_Bot2012_COI	YD40	COI	45	BB93644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD40	GYTAGTTCCRATGATGTTAGCTG	149.30462
BB93646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24498	WGS84	BB93646	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93646_Aamu_Bax2006_COI	YD36	1	BB93646_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93646	Sample	BB93646	targeted	Absent	Sample	Aamu_Bax2006_COI	YD36	COI	45	BB93646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD36	GCACAACCGGGATCTTTACTTC	149.30349
BB93646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24498	WGS84	BB93646	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93646_Pcan_Bot2011_IGS	YD36	1	BB93646_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93646	Sample	BB93646	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD36	IGS	45	BB93646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD36	CGTAATCCTCAGTACTGGCTA	149.30349
BB93646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24498	WGS84	BB93646	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93646_Sspa_Oph2007_COI	YD36	1	BB93646_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93646	Sample	BB93646	targeted	Absent	Sample	Sspa_Oph2007_COI	YD36	COI	45	BB93646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD36	GAAGGCCTTCCGATGATC	149.30349
BB93646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24498	WGS84	BB93646	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93646_Upin_Bot2011_COI	YD36	1	BB93646_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93646	Sample	BB93646	targeted	Absent	Sample	Upin_Bot2011_COI	YD36	COI	45	BB93646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD36	CTTTAATTACAGCGTTTTTATTGTTGT	149.30349
BB93647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24356	WGS84	BB93647	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93647_Mgig_Bot2012_COI	YD35	1	BB93647_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93647	Sample	BB93647	targeted	Absent	Sample	Mgig_Bot2012_COI	YD35	COI	45	BB93647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD35	TCTTATTCGTTGGAGACTTTATAACCCT	149.30339
BB93648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24414	WGS84	BB93648	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93648_Cmae_Bot2011_COI	YD34	1	BB93648_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93648	Sample	BB93648	targeted	Absent	Sample	Cmae_Bot2011_COI	YD34	COI	45	BB93648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD34	ATGAACAGTCTATCCTCCTTTAG	149.30384
BB93648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24414	WGS84	BB93648	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93648_Msal_Bot2012_COI	YD34	1	BB93648_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93648	Sample	BB93648	targeted	Absent	Sample	Msal_Bot2012_COI	YD34	COI	45	BB93648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD34	GYTAGTTCCRATGATGTTAGCTG	149.30384
BB93650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24972	WGS84	BB93650	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93650_Mgig_Bot2012_COI	YD24	1	BB93650_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93650	Sample	BB93650	targeted	Absent	Sample	Mgig_Bot2012_COI	YD24	COI	45	BB93650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD24	TCTTATTCGTTGGAGACTTTATAACCCT	149.30738
BB93651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24831	WGS84	BB93651	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93651_Cmae_Bot2011_COI	YD22	1	BB93651_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93651	Sample	BB93651	targeted	Absent	Sample	Cmae_Bot2011_COI	YD22	COI	45	BB93651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD22	ATGAACAGTCTATCCTCCTTTAG	149.30725
BB93651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24831	WGS84	BB93651	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93651_Msal_Bot2012_COI	YD22	1	BB93651_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93651	Sample	BB93651	targeted	Absent	Sample	Msal_Bot2012_COI	YD22	COI	45	BB93651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD22	GYTAGTTCCRATGATGTTAGCTG	149.30725
BB93653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24764	WGS84	BB93653	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93653_Aamu_Bax2006_COI	YD19	1	BB93653_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93653	Sample	BB93653	targeted	Absent	Sample	Aamu_Bax2006_COI	YD19	COI	45	BB93653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD19	GCACAACCGGGATCTTTACTTC	149.30671
BB93653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24764	WGS84	BB93653	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93653_Pcan_Bot2011_IGS	YD19	1	BB93653_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93653	Sample	BB93653	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD19	IGS	45	BB93653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD19	CGTAATCCTCAGTACTGGCTA	149.30671
BB93653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24764	WGS84	BB93653	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93653_Sspa_Oph2007_COI	YD19	1	BB93653_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93653	Sample	BB93653	targeted	Absent	Sample	Sspa_Oph2007_COI	YD19	COI	45	BB93653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD19	GAAGGCCTTCCGATGATC	149.30671
BB93653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24764	WGS84	BB93653	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93653_Upin_Bot2011_COI	YD19	1	BB93653_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93653	Sample	BB93653	targeted	Absent	Sample	Upin_Bot2011_COI	YD19	COI	45	BB93653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD19	CTTTAATTACAGCGTTTTTATTGTTGT	149.30671
BB93654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24845	WGS84	BB93654	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93654_Mgig_Bot2012_COI	YD18	1	BB93654_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93654	Sample	BB93654	targeted	Absent	Sample	Mgig_Bot2012_COI	YD18	COI	45	BB93654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD18	TCTTATTCGTTGGAGACTTTATAACCCT	149.3065
BB93655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24924	WGS84	BB93655	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93655_Cmae_Bot2011_COI	YD17	1	BB93655_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93655	Sample	BB93655	targeted	Absent	Sample	Cmae_Bot2011_COI	YD17	COI	45	BB93655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD17	ATGAACAGTCTATCCTCCTTTAG	149.30586
BB93655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24924	WGS84	BB93655	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93655_Msal_Bot2012_COI	YD17	1	BB93655_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93655	Sample	BB93655	targeted	Absent	Sample	Msal_Bot2012_COI	YD17	COI	45	BB93655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD17	GYTAGTTCCRATGATGTTAGCTG	149.30586
BB93657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24873	WGS84	BB93657	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93657_Aamu_Bax2006_COI	YD14	1	BB93657_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93657	Sample	BB93657	targeted	Absent	Sample	Aamu_Bax2006_COI	YD14	COI	45	BB93657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD14	GCACAACCGGGATCTTTACTTC	149.30351
BB93657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24873	WGS84	BB93657	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93657_Pcan_Bot2011_IGS	YD14	1	BB93657_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93657	Sample	BB93657	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD14	IGS	45	BB93657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD14	CGTAATCCTCAGTACTGGCTA	149.30351
BB93657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24873	WGS84	BB93657	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93657_Sspa_Oph2007_COI	YD14	1	BB93657_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93657	Sample	BB93657	targeted	Absent	Sample	Sspa_Oph2007_COI	YD14	COI	45	BB93657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD14	GAAGGCCTTCCGATGATC	149.30351
BB93657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24873	WGS84	BB93657	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93657_Upin_Bot2011_COI	YD14	1	BB93657_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93657	Sample	BB93657	targeted	Absent	Sample	Upin_Bot2011_COI	YD14	COI	45	BB93657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD14	CTTTAATTACAGCGTTTTTATTGTTGT	149.30351
BB93658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24742	WGS84	BB93658	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93658_Mgig_Bot2012_COI	YD12	1	BB93658_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93658	Sample	BB93658	targeted	Absent	Sample	Mgig_Bot2012_COI	YD12	COI	45	BB93658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD12	TCTTATTCGTTGGAGACTTTATAACCCT	149.30258
BB93659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24608	WGS84	BB93659	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93659_Cmae_Bot2011_COI	YD10	1	BB93659_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93659	Sample	BB93659	targeted	Absent	Sample	Cmae_Bot2011_COI	YD10	COI	45	BB93659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD10	ATGAACAGTCTATCCTCCTTTAG	149.30159
BB93659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24608	WGS84	BB93659	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93659_Msal_Bot2012_COI	YD10	1	BB93659_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93659	Sample	BB93659	targeted	Absent	Sample	Msal_Bot2012_COI	YD10	COI	45	BB93659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD10	GYTAGTTCCRATGATGTTAGCTG	149.30159
BB93660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24476	WGS84	BB93660	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93660_Aamu_Bax2006_COI	YD8	1	BB93660_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93660	Sample	BB93660	targeted	Absent	Sample	Aamu_Bax2006_COI	YD8	COI	45	BB93660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD8	GCACAACCGGGATCTTTACTTC	149.30136
BB93660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24476	WGS84	BB93660	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93660_Pcan_Bot2011_IGS	YD8	1	BB93660_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93660	Sample	BB93660	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD8	IGS	45	BB93660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD8	CGTAATCCTCAGTACTGGCTA	149.30136
BB93660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24476	WGS84	BB93660	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93660_Sspa_Oph2007_COI	YD8	1	BB93660_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93660	Sample	BB93660	targeted	Absent	Sample	Sspa_Oph2007_COI	YD8	COI	45	BB93660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD8	GAAGGCCTTCCGATGATC	149.30136
BB93660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24476	WGS84	BB93660	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93660_Upin_Bot2011_COI	YD8	1	BB93660_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93660	Sample	BB93660	targeted	Absent	Sample	Upin_Bot2011_COI	YD8	COI	45	BB93660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD8	CTTTAATTACAGCGTTTTTATTGTTGT	149.30136
BB93661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2443	WGS84	BB93661	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93661_Mgig_Bot2012_COI	YD7	1	BB93661_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93661	Sample	BB93661	targeted	Absent	Sample	Mgig_Bot2012_COI	YD7	COI	45	BB93661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD7	TCTTATTCGTTGGAGACTTTATAACCCT	149.30093
BB93662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2424	WGS84	BB93662	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93662_Cmae_Bot2011_COI	YD5	1	BB93662_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93662	Sample	BB93662	targeted	Absent	Sample	Cmae_Bot2011_COI	YD5	COI	45	BB93662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD5	ATGAACAGTCTATCCTCCTTTAG	149.30257
BB93662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2424	WGS84	BB93662	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93662_Msal_Bot2012_COI	YD5	1	BB93662_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93662	Sample	BB93662	targeted	Absent	Sample	Msal_Bot2012_COI	YD5	COI	45	BB93662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD5	GYTAGTTCCRATGATGTTAGCTG	149.30257
BB93664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24177	WGS84	BB93664	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93664_Aamu_Bax2006_COI	YD2	1	BB93664_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93664	Sample	BB93664	targeted	Absent	Sample	Aamu_Bax2006_COI	YD2	COI	45	BB93664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD2	GCACAACCGGGATCTTTACTTC	149.30205
BB93664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24177	WGS84	BB93664	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93664_Pcan_Bot2011_IGS	YD2	1	BB93664_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93664	Sample	BB93664	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD2	IGS	45	BB93664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD2	CGTAATCCTCAGTACTGGCTA	149.30205
BB93664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24177	WGS84	BB93664	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93664_Sspa_Oph2007_COI	YD2	1	BB93664_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93664	Sample	BB93664	targeted	Absent	Sample	Sspa_Oph2007_COI	YD2	COI	45	BB93664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD2	GAAGGCCTTCCGATGATC	149.30205
BB93664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24177	WGS84	BB93664	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93664_Upin_Bot2011_COI	YD2	1	BB93664_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93664	Sample	BB93664	targeted	Absent	Sample	Upin_Bot2011_COI	YD2	COI	45	BB93664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD2	CTTTAATTACAGCGTTTTTATTGTTGT	149.30205
BB93665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24181	WGS84	BB93665	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93665_Mgig_Bot2012_COI	YD1	1	BB93665_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93665	Sample	BB93665	targeted	Absent	Sample	Mgig_Bot2012_COI	YD1	COI	45	BB93665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD1	TCTTATTCGTTGGAGACTTTATAACCCT	149.30164
BB93666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93666	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.847	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93666_Cmae_Bot2011_COI	DD10	1	BB93666_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93666	Sample	BB93666	targeted	Present	Sample	Cmae_Bot2011_COI	DD10	COI	45	BB93666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD10	ATGAACAGTCTATCCTCCTTTAG	146.3657
BB93666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93666	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93666_Msal_Bot2012_COI	DD10	1	BB93666_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93666	Sample	BB93666	targeted	Absent	Sample	Msal_Bot2012_COI	DD10	COI	45	BB93666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD10	GYTAGTTCCRATGATGTTAGCTG	146.3657
BB93668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18778	WGS84	BB93668	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93668_Aamu_Bax2006_COI	DD6	1	BB93668_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93668	Sample	BB93668	targeted	Absent	Sample	Aamu_Bax2006_COI	DD6	COI	45	BB93668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD6	GCACAACCGGGATCTTTACTTC	146.36694
BB93668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18778	WGS84	BB93668	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93668_Pcan_Bot2011_IGS	DD6	1	BB93668_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93668	Sample	BB93668	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD6	IGS	45	BB93668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD6	CGTAATCCTCAGTACTGGCTA	146.36694
BB93668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18778	WGS84	BB93668	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93668_Sspa_Oph2007_COI	DD6	1	BB93668_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93668	Sample	BB93668	targeted	Absent	Sample	Sspa_Oph2007_COI	DD6	COI	45	BB93668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD6	GAAGGCCTTCCGATGATC	146.36694
BB93668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18778	WGS84	BB93668	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93668_Upin_Bot2011_COI	DD6	1	BB93668_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93668	Sample	BB93668	targeted	Absent	Sample	Upin_Bot2011_COI	DD6	COI	45	BB93668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD6	CTTTAATTACAGCGTTTTTATTGTTGT	146.36694
BB93669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.19181	WGS84	BB93669	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.754	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93669_Mgig_Bot2012_COI	DD1	1	BB93669_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93669	Sample	BB93669	targeted	Present	Sample	Mgig_Bot2012_COI	DD1	COI	45	BB93669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD1	TCTTATTCGTTGGAGACTTTATAACCCT	146.36808
BB93671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18763	WGS84	BB93671	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93671_Aamu_Bax2006_COI	DD5	1	BB93671_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93671	Sample	BB93671	targeted	Absent	Sample	Aamu_Bax2006_COI	DD5	COI	45	BB93671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD5	GCACAACCGGGATCTTTACTTC	146.36571
BB93671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18763	WGS84	BB93671	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93671_Pcan_Bot2011_IGS	DD5	1	BB93671_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93671	Sample	BB93671	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD5	IGS	45	BB93671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD5	CGTAATCCTCAGTACTGGCTA	146.36571
BB93671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18763	WGS84	BB93671	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93671_Sspa_Oph2007_COI	DD5	1	BB93671_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93671	Sample	BB93671	targeted	Absent	Sample	Sspa_Oph2007_COI	DD5	COI	45	BB93671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD5	GAAGGCCTTCCGATGATC	146.36571
BB93671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18763	WGS84	BB93671	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93671_Upin_Bot2011_COI	DD5	1	BB93671_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93671	Sample	BB93671	targeted	Absent	Sample	Upin_Bot2011_COI	DD5	COI	45	BB93671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD5	CTTTAATTACAGCGTTTTTATTGTTGT	146.36571
BB93672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93672	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93672_Mgig_Bot2012_COI	DD9	1	BB93672_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93672	Sample	BB93672	targeted	Absent	Sample	Mgig_Bot2012_COI	DD9	COI	45	BB93672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD9	TCTTATTCGTTGGAGACTTTATAACCCT	146.36416
BB93673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18359	WGS84	BB93673	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93673_Cmae_Bot2011_COI	DD13	1	BB93673_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93673	Sample	BB93673	targeted	Absent	Sample	Cmae_Bot2011_COI	DD13	COI	45	BB93673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD13	ATGAACAGTCTATCCTCCTTTAG	146.36547
BB93673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18359	WGS84	BB93673	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93673_Msal_Bot2012_COI	DD13	1	BB93673_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93673	Sample	BB93673	targeted	Absent	Sample	Msal_Bot2012_COI	DD13	COI	45	BB93673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD13	GYTAGTTCCRATGATGTTAGCTG	146.36547
BB93675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18176	WGS84	BB93675	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93675_Pcan_Bot2011_IGS	DD17	1	BB93675_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93675	Sample	BB93675	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD17	IGS	45	BB93675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD17	CGTAATCCTCAGTACTGGCTA	146.3652
BB93675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18176	WGS84	BB93675	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93675_Sspa_Oph2007_COI	DD17	1	BB93675_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93675	Sample	BB93675	targeted	Absent	Sample	Sspa_Oph2007_COI	DD17	COI	45	BB93675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD17	GAAGGCCTTCCGATGATC	146.3652
BB93675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18176	WGS84	BB93675	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93675_Upin_Bot2011_COI	DD17	1	BB93675_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93675	Sample	BB93675	targeted	Absent	Sample	Upin_Bot2011_COI	DD17	COI	45	BB93675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD17	CTTTAATTACAGCGTTTTTATTGTTGT	146.3652
BB93676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18283	WGS84	BB93676	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93676_Mgig_Bot2012_COI	DD15	1	BB93676_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93676	Sample	BB93676	targeted	Absent	Sample	Mgig_Bot2012_COI	DD15	COI	45	BB93676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD15	TCTTATTCGTTGGAGACTTTATAACCCT	146.36596
BB93677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18174	WGS84	BB93677	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.213	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93677_Cmae_Bot2011_COI	DD16	1	BB93677_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93677	Sample	BB93677	targeted	Present	Sample	Cmae_Bot2011_COI	DD16	COI	45	BB93677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD16	ATGAACAGTCTATCCTCCTTTAG	146.36395
BB93677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18174	WGS84	BB93677	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93677_Msal_Bot2012_COI	DD16	1	BB93677_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93677	Sample	BB93677	targeted	Absent	Sample	Msal_Bot2012_COI	DD16	COI	45	BB93677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD16	GYTAGTTCCRATGATGTTAGCTG	146.36395
BB93679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18084	WGS84	BB93679	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93679_Pcan_Bot2011_IGS	DD19	1	BB93679_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93679	Sample	BB93679	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD19	IGS	45	BB93679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD19	CGTAATCCTCAGTACTGGCTA	146.36514
BB93679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18084	WGS84	BB93679	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93679_Sspa_Oph2007_COI	DD19	1	BB93679_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93679	Sample	BB93679	targeted	Absent	Sample	Sspa_Oph2007_COI	DD19	COI	45	BB93679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD19	GAAGGCCTTCCGATGATC	146.36514
BB93679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18084	WGS84	BB93679	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93679_Upin_Bot2011_COI	DD19	1	BB93679_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93679	Sample	BB93679	targeted	Absent	Sample	Upin_Bot2011_COI	DD19	COI	45	BB93679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD19	CTTTAATTACAGCGTTTTTATTGTTGT	146.36514
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93680_Cmae_Bot2011_COI	VD5	1	BB93680_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Cmae_Bot2011_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	ATGAACAGTCTATCCTCCTTTAG	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93680_Hsan_Wil2021_COI	VD5	1	BB93680_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Hsan_Wil2021_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	CCTGGGCCGGTATAGTAGGT	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93680_Mros_Gib2022_COI	VD5	1	BB93680_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Mros_Gib2022_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93680_Msal_Bot2012_COI	VD5	1	BB93680_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Msal_Bot2012_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	GYTAGTTCCRATGATGTTAGCTG	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93680_Pvir_Dia2013_COI	VD5	1	BB93680_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Pvir_Dia2013_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	CTTAGTGGCATTAATTCGDAATCC	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93680_Rhar_Sim2018_COI	VD5	1	BB93680_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Rhar_Sim2018_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	CTGCTGCTATTGCTCATG	136.67224
BB93682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88291	WGS84	BB93682	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.878	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93682_Aamu_Bax2006_COI	HD19	1	BB93682_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93682	Sample	BB93682	targeted	Present	Sample	Aamu_Bax2006_COI	HD19	COI	45	BB93682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD19	GCACAACCGGGATCTTTACTTC	147.33838
BB93682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88291	WGS84	BB93682	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93682_Pcan_Bot2011_IGS	HD19	1	BB93682_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93682	Sample	BB93682	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD19	IGS	45	BB93682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD19	CGTAATCCTCAGTACTGGCTA	147.33838
BB93682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88291	WGS84	BB93682	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93682_Sspa_Oph2007_COI	HD19	1	BB93682_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93682	Sample	BB93682	targeted	Absent	Sample	Sspa_Oph2007_COI	HD19	COI	45	BB93682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD19	GAAGGCCTTCCGATGATC	147.33838
BB93682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88291	WGS84	BB93682	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93682_Upin_Bot2011_COI	HD19	1	BB93682_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93682	Sample	BB93682	targeted	Absent	Sample	Upin_Bot2011_COI	HD19	COI	45	BB93682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD19	CTTTAATTACAGCGTTTTTATTGTTGT	147.33838
BB93683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84434	WGS84	BB93683	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93683_Mgig_Bot2012_COI	HD6	1	BB93683_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93683	Sample	BB93683	targeted	Absent	Sample	Mgig_Bot2012_COI	HD6	COI	45	BB93683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD6	TCTTATTCGTTGGAGACTTTATAACCCT	147.32872
BB93684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84382	WGS84	BB93684	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.52	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93684_Cmae_Bot2011_COI	HD7	1	BB93684_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93684	Sample	BB93684	targeted	Present	Sample	Cmae_Bot2011_COI	HD7	COI	45	BB93684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD7	ATGAACAGTCTATCCTCCTTTAG	147.32637
BB93684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84382	WGS84	BB93684	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93684_Msal_Bot2012_COI	HD7	1	BB93684_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93684	Sample	BB93684	targeted	Absent	Sample	Msal_Bot2012_COI	HD7	COI	45	BB93684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD7	GYTAGTTCCRATGATGTTAGCTG	147.32637
BB93686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87278	WGS84	BB93686	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.19	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93686_Aamu_Bax2006_COI	HD9	1	BB93686_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93686	Sample	BB93686	targeted	Present	Sample	Aamu_Bax2006_COI	HD9	COI	45	BB93686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD9	GCACAACCGGGATCTTTACTTC	147.33622
BB93686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87278	WGS84	BB93686	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93686_Pcan_Bot2011_IGS	HD9	1	BB93686_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93686	Sample	BB93686	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD9	IGS	45	BB93686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD9	CGTAATCCTCAGTACTGGCTA	147.33622
BB93686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87278	WGS84	BB93686	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93686_Sspa_Oph2007_COI	HD9	1	BB93686_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93686	Sample	BB93686	targeted	Absent	Sample	Sspa_Oph2007_COI	HD9	COI	45	BB93686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD9	GAAGGCCTTCCGATGATC	147.33622
BB93686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87278	WGS84	BB93686	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93686_Upin_Bot2011_COI	HD9	1	BB93686_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93686	Sample	BB93686	targeted	Absent	Sample	Upin_Bot2011_COI	HD9	COI	45	BB93686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD9	CTTTAATTACAGCGTTTTTATTGTTGT	147.33622
BB93687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87576	WGS84	BB93687	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93687_Mgig_Bot2012_COI	HD11	1	BB93687_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93687	Sample	BB93687	targeted	Absent	Sample	Mgig_Bot2012_COI	HD11	COI	45	BB93687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33896
BB93688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8757	WGS84	BB93688	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93688_Cmae_Bot2011_COI	HD13	1	BB93688_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93688	Sample	BB93688	targeted	Absent	Sample	Cmae_Bot2011_COI	HD13	COI	45	BB93688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD13	ATGAACAGTCTATCCTCCTTTAG	147.34073
BB93688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8757	WGS84	BB93688	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93688_Msal_Bot2012_COI	HD13	1	BB93688_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93688	Sample	BB93688	targeted	Absent	Sample	Msal_Bot2012_COI	HD13	COI	45	BB93688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD13	GYTAGTTCCRATGATGTTAGCTG	147.34073
BB93690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88172	WGS84	BB93690	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93690_Mgig_Bot2012_COI	HD16	1	BB93690_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93690	Sample	BB93690	targeted	Absent	Sample	Mgig_Bot2012_COI	HD16	COI	45	BB93690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD16	TCTTATTCGTTGGAGACTTTATAACCCT	147.34203
BB93691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88517	WGS84	BB93691	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93691_Cmae_Bot2011_COI	HD17	1	BB93691_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93691	Sample	BB93691	targeted	Absent	Sample	Cmae_Bot2011_COI	HD17	COI	45	BB93691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD17	ATGAACAGTCTATCCTCCTTTAG	147.33462
BB93691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88517	WGS84	BB93691	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93691_Msal_Bot2012_COI	HD17	1	BB93691_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93691	Sample	BB93691	targeted	Absent	Sample	Msal_Bot2012_COI	HD17	COI	45	BB93691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD17	GYTAGTTCCRATGATGTTAGCTG	147.33462
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93693_Aamu_Bax2006_COI	VD10	1	BB93693_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Aamu_Bax2006_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	GCACAACCGGGATCTTTACTTC	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93693_Pcan_Bot2011_IGS	VD10	1	BB93693_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD10	IGS	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	CGTAATCCTCAGTACTGGCTA	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93693_Pper_Dia2013_COI	VD10	1	BB93693_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Pper_Dia2013_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	CTTAGTGGCATTAATTCGDAATCC	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93693_Sspa_Oph2007_COI	VD10	1	BB93693_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Sspa_Oph2007_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	GAAGGCCTTCCGATGATC	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93693_Upin_Bot2011_COI	VD10	1	BB93693_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Upin_Bot2011_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	CTTTAATTACAGCGTTTTTATTGTTGT	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20575	WGS84	BB93693	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93693_Vgib_Wil2025_COI	VD10	1	BB93693_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Vgib_Wil2025_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	CCCCTCTTTACAAGTTCTTTAGGGT	136.66871
BB93694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19539	WGS84	BB93694	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93694_Mgig_Bot2012_COI	VD1	1	BB93694_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93694	Sample	BB93694	targeted	Absent	Sample	Mgig_Bot2012_COI	VD1	COI	45	BB93694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD1	TCTTATTCGTTGGAGACTTTATAACCCT	136.68657
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93695_Cmae_Bot2011_COI	VD2	1	BB93695_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Cmae_Bot2011_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	ATGAACAGTCTATCCTCCTTTAG	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93695_Hsan_Wil2021_COI	VD2	1	BB93695_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Hsan_Wil2021_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	CCTGGGCCGGTATAGTAGGT	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93695_Mros_Gib2022_COI	VD2	1	BB93695_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Mros_Gib2022_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93695_Msal_Bot2012_COI	VD2	1	BB93695_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Msal_Bot2012_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	GYTAGTTCCRATGATGTTAGCTG	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93695_Pvir_Dia2013_COI	VD2	1	BB93695_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Pvir_Dia2013_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	CTTAGTGGCATTAATTCGDAATCC	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93695_Rhar_Sim2018_COI	VD2	1	BB93695_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Rhar_Sim2018_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	CTGCTGCTATTGCTCATG	136.68489
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93697_Aamu_Bax2006_COI	VD4	1	BB93697_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Aamu_Bax2006_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	GCACAACCGGGATCTTTACTTC	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93697_Pcan_Bot2011_IGS	VD4	1	BB93697_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD4	IGS	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	CGTAATCCTCAGTACTGGCTA	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93697_Pper_Dia2013_COI	VD4	1	BB93697_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Pper_Dia2013_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	CTTAGTGGCATTAATTCGDAATCC	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93697_Sspa_Oph2007_COI	VD4	1	BB93697_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Sspa_Oph2007_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	GAAGGCCTTCCGATGATC	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93697_Upin_Bot2011_COI	VD4	1	BB93697_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Upin_Bot2011_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	CTTTAATTACAGCGTTTTTATTGTTGT	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20645	WGS84	BB93697	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93697_Vgib_Wil2025_COI	VD4	1	BB93697_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Vgib_Wil2025_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	CCCCTCTTTACAAGTTCTTTAGGGT	136.67967
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20913	WGS84	BB93698	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93698_Asen_Wil2025_COI	VD18	1	BB93698_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Asen_Wil2025_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	GTTGTTGTTACAACACATGCTCTTTTG	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93698_Cjap_Sim2018_COI	VD18	1	BB93698_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Cjap_Sim2018_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93698_Esin_And2018_Cyb	VD18	1	BB93698_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Esin_And2018_Cyb	VD18	Cytb	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	ACCCCTCCTCATATCCAACCA	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93698_Mgig_Bot2012_COI	VD18	1	BB93698_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Mgig_Bot2012_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	TCTTATTCGTTGGAGACTTTATAACCCT	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93698_Mjap_Gib2022_COI	VD18	1	BB93698_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Mjap_Gib2022_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	AATTTAATTGTGACTAGGCATGGTCTG	136.66947
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93699_Cmae_Bot2011_COI	VD16	1	BB93699_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Cmae_Bot2011_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	ATGAACAGTCTATCCTCCTTTAG	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93699_Hsan_Wil2021_COI	VD16	1	BB93699_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Hsan_Wil2021_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	CCTGGGCCGGTATAGTAGGT	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93699_Mros_Gib2022_COI	VD16	1	BB93699_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Mros_Gib2022_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93699_Msal_Bot2012_COI	VD16	1	BB93699_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Msal_Bot2012_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	GYTAGTTCCRATGATGTTAGCTG	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93699_Pvir_Dia2013_COI	VD16	1	BB93699_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Pvir_Dia2013_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	CTTAGTGGCATTAATTCGDAATCC	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93699_Rhar_Sim2018_COI	VD16	1	BB93699_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Rhar_Sim2018_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	CTGCTGCTATTGCTCATG	136.66813
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93701_Aamu_Bax2006_COI	VD13	1	BB93701_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Aamu_Bax2006_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	GCACAACCGGGATCTTTACTTC	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93701_Pcan_Bot2011_IGS	VD13	1	BB93701_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD13	IGS	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	CGTAATCCTCAGTACTGGCTA	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93701_Pper_Dia2013_COI	VD13	1	BB93701_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Pper_Dia2013_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	CTTAGTGGCATTAATTCGDAATCC	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93701_Sspa_Oph2007_COI	VD13	1	BB93701_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Sspa_Oph2007_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	GAAGGCCTTCCGATGATC	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93701_Upin_Bot2011_COI	VD13	1	BB93701_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Upin_Bot2011_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	CTTTAATTACAGCGTTTTTATTGTTGT	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20918	WGS84	BB93701	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93701_Vgib_Wil2025_COI	VD13	1	BB93701_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Vgib_Wil2025_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	CCCCTCTTTACAAGTTCTTTAGGGT	136.67057
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20771	WGS84	BB93702	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93702_Asen_Wil2025_COI	VD12	1	BB93702_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Asen_Wil2025_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	GTTGTTGTTACAACACATGCTCTTTTG	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93702_Cjap_Sim2018_COI	VD12	1	BB93702_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Cjap_Sim2018_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93702_Esin_And2018_Cyb	VD12	1	BB93702_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Esin_And2018_Cyb	VD12	Cytb	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	ACCCCTCCTCATATCCAACCA	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93702_Mgig_Bot2012_COI	VD12	1	BB93702_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Mgig_Bot2012_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	TCTTATTCGTTGGAGACTTTATAACCCT	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93702_Mjap_Gib2022_COI	VD12	1	BB93702_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Mjap_Gib2022_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	AATTTAATTGTGACTAGGCATGGTCTG	136.6698
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93703_Cmae_Bot2011_COI	VD8	1	BB93703_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Cmae_Bot2011_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	ATGAACAGTCTATCCTCCTTTAG	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93703_Hsan_Wil2021_COI	VD8	1	BB93703_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Hsan_Wil2021_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	CCTGGGCCGGTATAGTAGGT	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93703_Mros_Gib2022_COI	VD8	1	BB93703_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Mros_Gib2022_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93703_Msal_Bot2012_COI	VD8	1	BB93703_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Msal_Bot2012_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	GYTAGTTCCRATGATGTTAGCTG	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93703_Pvir_Dia2013_COI	VD8	1	BB93703_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Pvir_Dia2013_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	CTTAGTGGCATTAATTCGDAATCC	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93703_Rhar_Sim2018_COI	VD8	1	BB93703_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Rhar_Sim2018_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	CTGCTGCTATTGCTCATG	136.6702
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93705_Aamu_Bax2006_COI	VD6	1	BB93705_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Aamu_Bax2006_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	GCACAACCGGGATCTTTACTTC	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93705_Pcan_Bot2011_IGS	VD6	1	BB93705_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD6	IGS	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	CGTAATCCTCAGTACTGGCTA	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93705_Pper_Dia2013_COI	VD6	1	BB93705_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Pper_Dia2013_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	CTTAGTGGCATTAATTCGDAATCC	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93705_Sspa_Oph2007_COI	VD6	1	BB93705_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Sspa_Oph2007_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	GAAGGCCTTCCGATGATC	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93705_Upin_Bot2011_COI	VD6	1	BB93705_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Upin_Bot2011_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	CTTTAATTACAGCGTTTTTATTGTTGT	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20707	WGS84	BB93705	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93705_Vgib_Wil2025_COI	VD6	1	BB93705_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Vgib_Wil2025_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	CCCCTCTTTACAAGTTCTTTAGGGT	136.67185
BB93706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84552	WGS84	BB93706	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93706_Mgig_Bot2012_COI	HD5	1	BB93706_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93706	Sample	BB93706	targeted	Absent	Sample	Mgig_Bot2012_COI	HD5	COI	45	BB93706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD5	TCTTATTCGTTGGAGACTTTATAACCCT	147.33115
BB93707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84389	WGS84	BB93707	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.689	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93707_Cmae_Bot2011_COI	HD4	1	BB93707_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93707	Sample	BB93707	targeted	Present	Sample	Cmae_Bot2011_COI	HD4	COI	45	BB93707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD4	ATGAACAGTCTATCCTCCTTTAG	147.33123
BB93707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84389	WGS84	BB93707	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93707_Msal_Bot2012_COI	HD4	1	BB93707_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93707	Sample	BB93707	targeted	Absent	Sample	Msal_Bot2012_COI	HD4	COI	45	BB93707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD4	GYTAGTTCCRATGATGTTAGCTG	147.33123
BB93709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82857	WGS84	BB93709	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93709_Aamu_Bax2006_COI	HD2	1	BB93709_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93709	Sample	BB93709	targeted	Absent	Sample	Aamu_Bax2006_COI	HD2	COI	45	BB93709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD2	GCACAACCGGGATCTTTACTTC	147.3172
BB93709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82857	WGS84	BB93709	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93709_Pcan_Bot2011_IGS	HD2	1	BB93709_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93709	Sample	BB93709	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD2	IGS	45	BB93709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD2	CGTAATCCTCAGTACTGGCTA	147.3172
BB93709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82857	WGS84	BB93709	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93709_Sspa_Oph2007_COI	HD2	1	BB93709_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93709	Sample	BB93709	targeted	Absent	Sample	Sspa_Oph2007_COI	HD2	COI	45	BB93709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD2	GAAGGCCTTCCGATGATC	147.3172
BB93709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82857	WGS84	BB93709	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93709_Upin_Bot2011_COI	HD2	1	BB93709_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93709	Sample	BB93709	targeted	Absent	Sample	Upin_Bot2011_COI	HD2	COI	45	BB93709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD2	CTTTAATTACAGCGTTTTTATTGTTGT	147.3172
BB93710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67457	WGS84	BB93710	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93710_Cmae_Bot2011_COI	WD15	1	BB93710_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93710	Sample	BB93710	targeted	Absent	Sample	Cmae_Bot2011_COI	WD15	COI	45	BB93710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD15	ATGAACAGTCTATCCTCCTTTAG	141.87024
BB93710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67457	WGS84	BB93710	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93710_Msal_Bot2012_COI	WD15	1	BB93710_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93710	Sample	BB93710	targeted	Absent	Sample	Msal_Bot2012_COI	WD15	COI	45	BB93710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD15	GYTAGTTCCRATGATGTTAGCTG	141.87024
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93712_Aamu_Bax2006_COI	WD3	1	BB93712_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Aamu_Bax2006_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	GCACAACCGGGATCTTTACTTC	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93712_Pcan_Bot2011_IGS	WD3	1	BB93712_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD3	IGS	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	CGTAATCCTCAGTACTGGCTA	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93712_Pper_Dia2013_COI	WD3	1	BB93712_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Pper_Dia2013_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	CTTAGTGGCATTAATTCGDAATCC	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93712_Sspa_Oph2007_COI	WD3	1	BB93712_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Sspa_Oph2007_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	GAAGGCCTTCCGATGATC	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93712_Upin_Bot2011_COI	WD3	1	BB93712_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Upin_Bot2011_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	CTTTAATTACAGCGTTTTTATTGTTGT	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66714	WGS84	BB93712	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93712_Vgib_Wil2025_COI	WD3	1	BB93712_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Vgib_Wil2025_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	CCCCTCTTTACAAGTTCTTTAGGGT	141.84936
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66603	WGS84	BB93713	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93713_Asen_Wil2025_COI	WD4	1	BB93713_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Asen_Wil2025_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	GTTGTTGTTACAACACATGCTCTTTTG	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93713_Cjap_Sim2018_COI	WD4	1	BB93713_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Cjap_Sim2018_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93713_Esin_And2018_Cyb	WD4	1	BB93713_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Esin_And2018_Cyb	WD4	Cytb	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	ACCCCTCCTCATATCCAACCA	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93713_Mgig_Bot2012_COI	WD4	1	BB93713_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Mgig_Bot2012_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	TCTTATTCGTTGGAGACTTTATAACCCT	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93713_Mjap_Gib2022_COI	WD4	1	BB93713_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Mjap_Gib2022_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	AATTTAATTGTGACTAGGCATGGTCTG	141.84863
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93714_Cmae_Bot2011_COI	WD7	1	BB93714_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Cmae_Bot2011_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	ATGAACAGTCTATCCTCCTTTAG	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93714_Hsan_Wil2021_COI	WD7	1	BB93714_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Hsan_Wil2021_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	CCTGGGCCGGTATAGTAGGT	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93714_Mros_Gib2022_COI	WD7	1	BB93714_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Mros_Gib2022_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93714_Msal_Bot2012_COI	WD7	1	BB93714_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Msal_Bot2012_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	GYTAGTTCCRATGATGTTAGCTG	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93714_Pvir_Dia2013_COI	WD7	1	BB93714_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Pvir_Dia2013_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	CTTAGTGGCATTAATTCGDAATCC	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93714_Rhar_Sim2018_COI	WD7	1	BB93714_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Rhar_Sim2018_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	CTGCTGCTATTGCTCATG	141.86098
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93716_Aamu_Bax2006_COI	WD9	1	BB93716_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Aamu_Bax2006_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	GCACAACCGGGATCTTTACTTC	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93716_Pcan_Bot2011_IGS	WD9	1	BB93716_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD9	IGS	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	CGTAATCCTCAGTACTGGCTA	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93716_Pper_Dia2013_COI	WD9	1	BB93716_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Pper_Dia2013_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	CTTAGTGGCATTAATTCGDAATCC	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93716_Sspa_Oph2007_COI	WD9	1	BB93716_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Sspa_Oph2007_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	GAAGGCCTTCCGATGATC	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93716_Upin_Bot2011_COI	WD9	1	BB93716_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Upin_Bot2011_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	CTTTAATTACAGCGTTTTTATTGTTGT	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66985	WGS84	BB93716	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93716_Vgib_Wil2025_COI	WD9	1	BB93716_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Vgib_Wil2025_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	CCCCTCTTTACAAGTTCTTTAGGGT	141.86396
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67253	WGS84	BB93717	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93717_Asen_Wil2025_COI	WD11	1	BB93717_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Asen_Wil2025_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	GTTGTTGTTACAACACATGCTCTTTTG	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93717_Cjap_Sim2018_COI	WD11	1	BB93717_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Cjap_Sim2018_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93717_Esin_And2018_Cyb	WD11	1	BB93717_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Esin_And2018_Cyb	WD11	Cytb	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	ACCCCTCCTCATATCCAACCA	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93717_Mgig_Bot2012_COI	WD11	1	BB93717_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Mgig_Bot2012_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	TCTTATTCGTTGGAGACTTTATAACCCT	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93717_Mjap_Gib2022_COI	WD11	1	BB93717_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Mjap_Gib2022_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	AATTTAATTGTGACTAGGCATGGTCTG	141.86743
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93718_Cmae_Bot2011_COI	WD12	1	BB93718_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Cmae_Bot2011_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	ATGAACAGTCTATCCTCCTTTAG	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93718_Hsan_Wil2021_COI	WD12	1	BB93718_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Hsan_Wil2021_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	CCTGGGCCGGTATAGTAGGT	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93718_Mros_Gib2022_COI	WD12	1	BB93718_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Mros_Gib2022_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93718_Msal_Bot2012_COI	WD12	1	BB93718_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Msal_Bot2012_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	GYTAGTTCCRATGATGTTAGCTG	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93718_Pvir_Dia2013_COI	WD12	1	BB93718_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Pvir_Dia2013_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	CTTAGTGGCATTAATTCGDAATCC	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93718_Rhar_Sim2018_COI	WD12	1	BB93718_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Rhar_Sim2018_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	CTGCTGCTATTGCTCATG	141.86683
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67469	WGS84	BB93720	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93720_Asen_Wil2025_COI	WD16	1	BB93720_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Asen_Wil2025_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	GTTGTTGTTACAACACATGCTCTTTTG	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93720_Cjap_Sim2018_COI	WD16	1	BB93720_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Cjap_Sim2018_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93720_Esin_And2018_Cyb	WD16	1	BB93720_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Esin_And2018_Cyb	WD16	Cytb	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	ACCCCTCCTCATATCCAACCA	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93720_Mgig_Bot2012_COI	WD16	1	BB93720_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Mgig_Bot2012_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	TCTTATTCGTTGGAGACTTTATAACCCT	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93720_Mjap_Gib2022_COI	WD16	1	BB93720_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Mjap_Gib2022_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	AATTTAATTGTGACTAGGCATGGTCTG	141.86884
BB93721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67454	WGS84	BB93721	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93721_Cmae_Bot2011_COI	WD18	1	BB93721_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93721	Sample	BB93721	targeted	Absent	Sample	Cmae_Bot2011_COI	WD18	COI	45	BB93721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD18	ATGAACAGTCTATCCTCCTTTAG	141.87152
BB93721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67454	WGS84	BB93721	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93721_Msal_Bot2012_COI	WD18	1	BB93721_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93721	Sample	BB93721	targeted	Absent	Sample	Msal_Bot2012_COI	WD18	COI	45	BB93721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD18	GYTAGTTCCRATGATGTTAGCTG	141.87152
BB93723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66582	WGS84	BB93723	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93723_Aamu_Bax2006_COI	WD5	1	BB93723_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93723	Sample	BB93723	targeted	Absent	Sample	Aamu_Bax2006_COI	WD5	COI	45	BB93723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD5	GCACAACCGGGATCTTTACTTC	141.85
BB93723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66582	WGS84	BB93723	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93723_Pcan_Bot2011_IGS	WD5	1	BB93723_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93723	Sample	BB93723	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD5	IGS	45	BB93723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD5	CGTAATCCTCAGTACTGGCTA	141.85
BB93723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66582	WGS84	BB93723	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93723_Sspa_Oph2007_COI	WD5	1	BB93723_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93723	Sample	BB93723	targeted	Absent	Sample	Sspa_Oph2007_COI	WD5	COI	45	BB93723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD5	GAAGGCCTTCCGATGATC	141.85
BB93723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66582	WGS84	BB93723	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93723_Upin_Bot2011_COI	WD5	1	BB93723_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93723	Sample	BB93723	targeted	Absent	Sample	Upin_Bot2011_COI	WD5	COI	45	BB93723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD5	CTTTAATTACAGCGTTTTTATTGTTGT	141.85
BB93724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67261	WGS84	BB93724	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93724_Mgig_Bot2012_COI	WD10	1	BB93724_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93724	Sample	BB93724	targeted	Absent	Sample	Mgig_Bot2012_COI	WD10	COI	45	BB93724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD10	TCTTATTCGTTGGAGACTTTATAACCCT	141.86627
BB93725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81853	WGS84	BB93725	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93725_Cmae_Bot2011_COI	MC75	1	BB93725_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93725	Sample	BB93725	targeted	Absent	Sample	Cmae_Bot2011_COI	MC75	COI	45	BB93725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC75	ATGAACAGTCTATCCTCCTTTAG	144.92784
BB93725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81853	WGS84	BB93725	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93725_Msal_Bot2012_COI	MC75	1	BB93725_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93725	Sample	BB93725	targeted	Absent	Sample	Msal_Bot2012_COI	MC75	COI	45	BB93725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC75	GYTAGTTCCRATGATGTTAGCTG	144.92784
BB93727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82836	WGS84	BB93727	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93727_Aamu_Bax2006_COI	MC50	1	BB93727_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93727	Sample	BB93727	targeted	Absent	Sample	Aamu_Bax2006_COI	MC50	COI	45	BB93727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC50	GCACAACCGGGATCTTTACTTC	144.89947
BB93727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82836	WGS84	BB93727	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93727_Pcan_Bot2011_IGS	MC50	1	BB93727_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93727	Sample	BB93727	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC50	IGS	45	BB93727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC50	CGTAATCCTCAGTACTGGCTA	144.89947
BB93727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82836	WGS84	BB93727	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.982	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93727_Sspa_Oph2007_COI	MC50	1	BB93727_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93727	Sample	BB93727	targeted	Present	Sample	Sspa_Oph2007_COI	MC50	COI	45	BB93727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC50	GAAGGCCTTCCGATGATC	144.89947
BB93727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82836	WGS84	BB93727	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93727_Upin_Bot2011_COI	MC50	1	BB93727_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93727	Sample	BB93727	targeted	Absent	Sample	Upin_Bot2011_COI	MC50	COI	45	BB93727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC50	CTTTAATTACAGCGTTTTTATTGTTGT	144.89947
BB93728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86071	WGS84	BB93728	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.403	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93728_Mgig_Bot2012_COI	MC45	1	BB93728_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93728	Sample	BB93728	targeted	Present	Sample	Mgig_Bot2012_COI	MC45	COI	45	BB93728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC45	TCTTATTCGTTGGAGACTTTATAACCCT	144.9137
BB93729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86215	WGS84	BB93729	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93729_Cmae_Bot2011_COI	MC40	1	BB93729_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93729	Sample	BB93729	targeted	Absent	Sample	Cmae_Bot2011_COI	MC40	COI	45	BB93729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC40	ATGAACAGTCTATCCTCCTTTAG	144.91262
BB93729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86215	WGS84	BB93729	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93729_Msal_Bot2012_COI	MC40	1	BB93729_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93729	Sample	BB93729	targeted	Absent	Sample	Msal_Bot2012_COI	MC40	COI	45	BB93729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC40	GYTAGTTCCRATGATGTTAGCTG	144.91262
BB93730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84891	WGS84	BB93730	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93730_Aamu_Bax2006_COI	MC20	1	BB93730_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93730	Sample	BB93730	targeted	Absent	Sample	Aamu_Bax2006_COI	MC20	COI	45	BB93730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC20	GCACAACCGGGATCTTTACTTC	144.91502
BB93730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84891	WGS84	BB93730	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93730_Pcan_Bot2011_IGS	MC20	1	BB93730_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93730	Sample	BB93730	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC20	IGS	45	BB93730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC20	CGTAATCCTCAGTACTGGCTA	144.91502
BB93730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84891	WGS84	BB93730	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.699	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93730_Sspa_Oph2007_COI	MC20	1	BB93730_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93730	Sample	BB93730	targeted	Present	Sample	Sspa_Oph2007_COI	MC20	COI	45	BB93730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC20	GAAGGCCTTCCGATGATC	144.91502
BB93730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84891	WGS84	BB93730	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93730_Upin_Bot2011_COI	MC20	1	BB93730_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93730	Sample	BB93730	targeted	Absent	Sample	Upin_Bot2011_COI	MC20	COI	45	BB93730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC20	CTTTAATTACAGCGTTTTTATTGTTGT	144.91502
BB93731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8489	WGS84	BB93731	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.656	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93731_Mgig_Bot2012_COI	MC15	1	BB93731_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93731	Sample	BB93731	targeted	Present	Sample	Mgig_Bot2012_COI	MC15	COI	45	BB93731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC15	TCTTATTCGTTGGAGACTTTATAACCCT	144.91028
BB93732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81862	WGS84	BB93732	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93732_Cmae_Bot2011_COI	MC85	1	BB93732_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93732	Sample	BB93732	targeted	Absent	Sample	Cmae_Bot2011_COI	MC85	COI	45	BB93732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC85	ATGAACAGTCTATCCTCCTTTAG	144.92195
BB93732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81862	WGS84	BB93732	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93732_Msal_Bot2012_COI	MC85	1	BB93732_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93732	Sample	BB93732	targeted	Absent	Sample	Msal_Bot2012_COI	MC85	COI	45	BB93732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC85	GYTAGTTCCRATGATGTTAGCTG	144.92195
BB93734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82131	WGS84	BB93734	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93734_Aamu_Bax2006_COI	MC115	1	BB93734_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93734	Sample	BB93734	targeted	Absent	Sample	Aamu_Bax2006_COI	MC115	COI	45	BB93734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC115	GCACAACCGGGATCTTTACTTC	144.90843
BB93734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82131	WGS84	BB93734	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93734_Pcan_Bot2011_IGS	MC115	1	BB93734_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93734	Sample	BB93734	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC115	IGS	45	BB93734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC115	CGTAATCCTCAGTACTGGCTA	144.90843
BB93734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82131	WGS84	BB93734	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.486	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93734_Sspa_Oph2007_COI	MC115	1	BB93734_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93734	Sample	BB93734	targeted	Present	Sample	Sspa_Oph2007_COI	MC115	COI	45	BB93734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC115	GAAGGCCTTCCGATGATC	144.90843
BB93734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82131	WGS84	BB93734	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93734_Upin_Bot2011_COI	MC115	1	BB93734_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93734	Sample	BB93734	targeted	Absent	Sample	Upin_Bot2011_COI	MC115	COI	45	BB93734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC115	CTTTAATTACAGCGTTTTTATTGTTGT	144.90843
BB93735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93735	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.915	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93735_Mgig_Bot2012_COI	MC110	1	BB93735_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93735	Sample	BB93735	targeted	Present	Sample	Mgig_Bot2012_COI	MC110	COI	45	BB93735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC110	TCTTATTCGTTGGAGACTTTATAACCCT	144.91218
BB93736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81673	WGS84	BB93736	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93736_Cmae_Bot2011_COI	MC78	1	BB93736_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93736	Sample	BB93736	targeted	Absent	Sample	Cmae_Bot2011_COI	MC78	COI	45	BB93736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC78	ATGAACAGTCTATCCTCCTTTAG	144.92566
BB93736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81673	WGS84	BB93736	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93736_Msal_Bot2012_COI	MC78	1	BB93736_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93736	Sample	BB93736	targeted	Absent	Sample	Msal_Bot2012_COI	MC78	COI	45	BB93736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC78	GYTAGTTCCRATGATGTTAGCTG	144.92566
BB93738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81989	WGS84	BB93738	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93738_Aamu_Bax2006_COI	MC73	1	BB93738_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93738	Sample	BB93738	targeted	Absent	Sample	Aamu_Bax2006_COI	MC73	COI	45	BB93738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC73	GCACAACCGGGATCTTTACTTC	144.92932
BB93738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81989	WGS84	BB93738	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93738_Pcan_Bot2011_IGS	MC73	1	BB93738_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93738	Sample	BB93738	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC73	IGS	45	BB93738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC73	CGTAATCCTCAGTACTGGCTA	144.92932
BB93738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81989	WGS84	BB93738	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.566	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93738_Sspa_Oph2007_COI	MC73	1	BB93738_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93738	Sample	BB93738	targeted	Present	Sample	Sspa_Oph2007_COI	MC73	COI	45	BB93738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC73	GAAGGCCTTCCGATGATC	144.92932
BB93738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81989	WGS84	BB93738	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93738_Upin_Bot2011_COI	MC73	1	BB93738_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93738	Sample	BB93738	targeted	Absent	Sample	Upin_Bot2011_COI	MC73	COI	45	BB93738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC73	CTTTAATTACAGCGTTTTTATTGTTGT	144.92932
BB93739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82006	WGS84	BB93739	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.779	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93739_Mgig_Bot2012_COI	MC72	1	BB93739_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93739	Sample	BB93739	targeted	Present	Sample	Mgig_Bot2012_COI	MC72	COI	45	BB93739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC72	TCTTATTCGTTGGAGACTTTATAACCCT	144.93236
BB93741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81929	WGS84	BB93741	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93741_Aamu_Bax2006_COI	MC66	1	BB93741_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93741	Sample	BB93741	targeted	Absent	Sample	Aamu_Bax2006_COI	MC66	COI	45	BB93741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC66	GCACAACCGGGATCTTTACTTC	144.91866
BB93741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81929	WGS84	BB93741	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93741_Pcan_Bot2011_IGS	MC66	1	BB93741_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93741	Sample	BB93741	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC66	IGS	45	BB93741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC66	CGTAATCCTCAGTACTGGCTA	144.91866
BB93741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81929	WGS84	BB93741	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.097	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93741_Sspa_Oph2007_COI	MC66	1	BB93741_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93741	Sample	BB93741	targeted	Present	Sample	Sspa_Oph2007_COI	MC66	COI	45	BB93741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC66	GAAGGCCTTCCGATGATC	144.91866
BB93741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81929	WGS84	BB93741	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93741_Upin_Bot2011_COI	MC66	1	BB93741_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93741	Sample	BB93741	targeted	Absent	Sample	Upin_Bot2011_COI	MC66	COI	45	BB93741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC66	CTTTAATTACAGCGTTTTTATTGTTGT	144.91866
BB93742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81982	WGS84	BB93742	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.007	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93742_Mgig_Bot2012_COI	MC64	1	BB93742_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93742	Sample	BB93742	targeted	Present	Sample	Mgig_Bot2012_COI	MC64	COI	45	BB93742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC64	TCTTATTCGTTGGAGACTTTATAACCCT	144.91739
BB93743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82302	WGS84	BB93743	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93743_Cmae_Bot2011_COI	MC62	1	BB93743_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93743	Sample	BB93743	targeted	Absent	Sample	Cmae_Bot2011_COI	MC62	COI	45	BB93743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC62	ATGAACAGTCTATCCTCCTTTAG	144.90666
BB93743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82302	WGS84	BB93743	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93743_Msal_Bot2012_COI	MC62	1	BB93743_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93743	Sample	BB93743	targeted	Absent	Sample	Msal_Bot2012_COI	MC62	COI	45	BB93743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC62	GYTAGTTCCRATGATGTTAGCTG	144.90666
BB93745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82512	WGS84	BB93745	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93745_Aamu_Bax2006_COI	MC53	1	BB93745_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93745	Sample	BB93745	targeted	Absent	Sample	Aamu_Bax2006_COI	MC53	COI	45	BB93745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC53	GCACAACCGGGATCTTTACTTC	144.90113
BB93745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82512	WGS84	BB93745	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93745_Pcan_Bot2011_IGS	MC53	1	BB93745_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93745	Sample	BB93745	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC53	IGS	45	BB93745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC53	CGTAATCCTCAGTACTGGCTA	144.90113
BB93745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82512	WGS84	BB93745	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.73	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93745_Sspa_Oph2007_COI	MC53	1	BB93745_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93745	Sample	BB93745	targeted	Present	Sample	Sspa_Oph2007_COI	MC53	COI	45	BB93745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC53	GAAGGCCTTCCGATGATC	144.90113
BB93745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82512	WGS84	BB93745	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93745_Upin_Bot2011_COI	MC53	1	BB93745_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93745	Sample	BB93745	targeted	Absent	Sample	Upin_Bot2011_COI	MC53	COI	45	BB93745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC53	CTTTAATTACAGCGTTTTTATTGTTGT	144.90113
BB93746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82613	WGS84	BB93746	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.074	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93746_Mgig_Bot2012_COI	MC52	1	BB93746_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93746	Sample	BB93746	targeted	Present	Sample	Mgig_Bot2012_COI	MC52	COI	45	BB93746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC52	TCTTATTCGTTGGAGACTTTATAACCCT	144.9012
BB93747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8609	WGS84	BB93747	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93747_Cmae_Bot2011_COI	MC48	1	BB93747_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93747	Sample	BB93747	targeted	Absent	Sample	Cmae_Bot2011_COI	MC48	COI	45	BB93747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC48	ATGAACAGTCTATCCTCCTTTAG	144.91203
BB93747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8609	WGS84	BB93747	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93747_Msal_Bot2012_COI	MC48	1	BB93747_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93747	Sample	BB93747	targeted	Absent	Sample	Msal_Bot2012_COI	MC48	COI	45	BB93747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC48	GYTAGTTCCRATGATGTTAGCTG	144.91203
BB93749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86107	WGS84	BB93749	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.28	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93749_Aamu_Bax2006_COI	MC46	1	BB93749_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93749	Sample	BB93749	targeted	Present	Sample	Aamu_Bax2006_COI	MC46	COI	45	BB93749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC46	GCACAACCGGGATCTTTACTTC	144.91072
BB93749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86107	WGS84	BB93749	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93749_Pcan_Bot2011_IGS	MC46	1	BB93749_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93749	Sample	BB93749	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC46	IGS	45	BB93749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC46	CGTAATCCTCAGTACTGGCTA	144.91072
BB93749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86107	WGS84	BB93749	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.434	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93749_Sspa_Oph2007_COI	MC46	1	BB93749_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93749	Sample	BB93749	targeted	Present	Sample	Sspa_Oph2007_COI	MC46	COI	45	BB93749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC46	GAAGGCCTTCCGATGATC	144.91072
BB93749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86107	WGS84	BB93749	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93749_Upin_Bot2011_COI	MC46	1	BB93749_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93749	Sample	BB93749	targeted	Absent	Sample	Upin_Bot2011_COI	MC46	COI	45	BB93749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC46	CTTTAATTACAGCGTTTTTATTGTTGT	144.91072
BB93750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86157	WGS84	BB93750	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93750_Cmae_Bot2011_COI	MC44	1	BB93750_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93750	Sample	BB93750	targeted	Absent	Sample	Cmae_Bot2011_COI	MC44	COI	45	BB93750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC44	ATGAACAGTCTATCCTCCTTTAG	144.91759
BB93750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86157	WGS84	BB93750	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93750_Msal_Bot2012_COI	MC44	1	BB93750_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93750	Sample	BB93750	targeted	Absent	Sample	Msal_Bot2012_COI	MC44	COI	45	BB93750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC44	GYTAGTTCCRATGATGTTAGCTG	144.91759
BB93752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86221	WGS84	BB93752	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.245	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93752_Aamu_Bax2006_COI	MC42	1	BB93752_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93752	Sample	BB93752	targeted	Present	Sample	Aamu_Bax2006_COI	MC42	COI	45	BB93752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC42	GCACAACCGGGATCTTTACTTC	144.91719
BB93752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86221	WGS84	BB93752	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93752_Pcan_Bot2011_IGS	MC42	1	BB93752_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93752	Sample	BB93752	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC42	IGS	45	BB93752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC42	CGTAATCCTCAGTACTGGCTA	144.91719
BB93752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86221	WGS84	BB93752	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.518	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93752_Sspa_Oph2007_COI	MC42	1	BB93752_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93752	Sample	BB93752	targeted	Present	Sample	Sspa_Oph2007_COI	MC42	COI	45	BB93752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC42	GAAGGCCTTCCGATGATC	144.91719
BB93752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86221	WGS84	BB93752	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.247	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93752_Upin_Bot2011_COI	MC42	1	BB93752_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93752	Sample	BB93752	targeted	Present	Sample	Upin_Bot2011_COI	MC42	COI	45	BB93752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC42	CTTTAATTACAGCGTTTTTATTGTTGT	144.91719
BB93753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93753	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.644	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93753_Mgig_Bot2012_COI	MC41	1	BB93753_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93753	Sample	BB93753	targeted	Present	Sample	Mgig_Bot2012_COI	MC41	COI	45	BB93753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC41	TCTTATTCGTTGGAGACTTTATAACCCT	144.91334
BB93754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86172	WGS84	BB93754	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93754_Cmae_Bot2011_COI	MC39	1	BB93754_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93754	Sample	BB93754	targeted	Absent	Sample	Cmae_Bot2011_COI	MC39	COI	45	BB93754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC39	ATGAACAGTCTATCCTCCTTTAG	144.91464
BB93754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86172	WGS84	BB93754	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93754_Msal_Bot2012_COI	MC39	1	BB93754_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93754	Sample	BB93754	targeted	Absent	Sample	Msal_Bot2012_COI	MC39	COI	45	BB93754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC39	GYTAGTTCCRATGATGTTAGCTG	144.91464
BB93756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86366	WGS84	BB93756	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93756_Aamu_Bax2006_COI	MC37	1	BB93756_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93756	Sample	BB93756	targeted	Absent	Sample	Aamu_Bax2006_COI	MC37	COI	45	BB93756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC37	GCACAACCGGGATCTTTACTTC	144.91806
BB93756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86366	WGS84	BB93756	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93756_Pcan_Bot2011_IGS	MC37	1	BB93756_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93756	Sample	BB93756	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC37	IGS	45	BB93756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC37	CGTAATCCTCAGTACTGGCTA	144.91806
BB93756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86366	WGS84	BB93756	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.255	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93756_Sspa_Oph2007_COI	MC37	1	BB93756_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93756	Sample	BB93756	targeted	Present	Sample	Sspa_Oph2007_COI	MC37	COI	45	BB93756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC37	GAAGGCCTTCCGATGATC	144.91806
BB93756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86366	WGS84	BB93756	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.371	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93756_Upin_Bot2011_COI	MC37	1	BB93756_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93756	Sample	BB93756	targeted	Present	Sample	Upin_Bot2011_COI	MC37	COI	45	BB93756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC37	CTTTAATTACAGCGTTTTTATTGTTGT	144.91806
BB93757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86471	WGS84	BB93757	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.469	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93757_Mgig_Bot2012_COI	MC36	1	BB93757_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93757	Sample	BB93757	targeted	Present	Sample	Mgig_Bot2012_COI	MC36	COI	45	BB93757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC36	TCTTATTCGTTGGAGACTTTATAACCCT	144.91845
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93758_Cmae_Bot2011_COI	MC33	1	BB93758_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Absent	Sample	Cmae_Bot2011_COI	MC33	COI	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	ATGAACAGTCTATCCTCCTTTAG	144.91624
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	38.8543025	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93758_Hsan_Wil2021_COI	MC33	1	BB93758_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Present	Sample	Hsan_Wil2021_COI	MC33	COI	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	CCTGGGCCGGTATAGTAGGT	144.91624
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93758_Msal_Bot2012_COI	MC33	1	BB93758_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Absent	Sample	Msal_Bot2012_COI	MC33	COI	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	GYTAGTTCCRATGATGTTAGCTG	144.91624
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.549	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93760_Mgig_Bot2012_COI	MC32	1	BB93760_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Present	Sample	Mgig_Bot2012_COI	MC32	COI	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	TCTTATTCGTTGGAGACTTTATAACCCT	144.91742
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93761_Cmae_Bot2011_COI	MC29	1	BB93761_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Absent	Sample	Cmae_Bot2011_COI	MC29	COI	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	ATGAACAGTCTATCCTCCTTTAG	144.91673
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93761_Hsan_Wil2021_COI	MC29	1	BB93761_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Absent	Sample	Hsan_Wil2021_COI	MC29	COI	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	CCTGGGCCGGTATAGTAGGT	144.91673
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93761_Msal_Bot2012_COI	MC29	1	BB93761_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Absent	Sample	Msal_Bot2012_COI	MC29	COI	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	GYTAGTTCCRATGATGTTAGCTG	144.91673
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93763_Aamu_Bax2006_COI	MC24	1	BB93763_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Absent	Sample	Aamu_Bax2006_COI	MC24	COI	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	GCACAACCGGGATCTTTACTTC	144.90579
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93763_Pcan_Bot2011_IGS	MC24	1	BB93763_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC24	IGS	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	CGTAATCCTCAGTACTGGCTA	144.90579
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.362	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93763_Sspa_Oph2007_COI	MC24	1	BB93763_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Present	Sample	Sspa_Oph2007_COI	MC24	COI	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	GAAGGCCTTCCGATGATC	144.90579
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93763_Upin_Bot2011_COI	MC24	1	BB93763_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Absent	Sample	Upin_Bot2011_COI	MC24	COI	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	CTTTAATTACAGCGTTTTTATTGTTGT	144.90579
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.461	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93764_Mgig_Bot2012_COI	MC22	1	BB93764_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Present	Sample	Mgig_Bot2012_COI	MC22	COI	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	TCTTATTCGTTGGAGACTTTATAACCCT	144.90784
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93765_Cmae_Bot2011_COI	MC17	1	BB93765_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Absent	Sample	Cmae_Bot2011_COI	MC17	COI	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	ATGAACAGTCTATCCTCCTTTAG	144.91022
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93765_Hsan_Wil2021_COI	MC17	1	BB93765_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Absent	Sample	Hsan_Wil2021_COI	MC17	COI	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	CCTGGGCCGGTATAGTAGGT	144.91022
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93765_Msal_Bot2012_COI	MC17	1	BB93765_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Absent	Sample	Msal_Bot2012_COI	MC17	COI	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	GYTAGTTCCRATGATGTTAGCTG	144.91022
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93767_Aamu_Bax2006_COI	MC13	1	BB93767_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Absent	Sample	Aamu_Bax2006_COI	MC13	COI	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	GCACAACCGGGATCTTTACTTC	144.91209
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93767_Pcan_Bot2011_IGS	MC13	1	BB93767_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC13	IGS	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	CGTAATCCTCAGTACTGGCTA	144.91209
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.571	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93767_Sspa_Oph2007_COI	MC13	1	BB93767_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Present	Sample	Sspa_Oph2007_COI	MC13	COI	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	GAAGGCCTTCCGATGATC	144.91209
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93767_Upin_Bot2011_COI	MC13	1	BB93767_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Absent	Sample	Upin_Bot2011_COI	MC13	COI	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	CTTTAATTACAGCGTTTTTATTGTTGT	144.91209
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.816	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93768_Mgig_Bot2012_COI	MC12	1	BB93768_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Present	Sample	Mgig_Bot2012_COI	MC12	COI	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	TCTTATTCGTTGGAGACTTTATAACCCT	144.91052
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93769_Cmae_Bot2011_COI	MC9	1	BB93769_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Absent	Sample	Cmae_Bot2011_COI	MC9	COI	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	ATGAACAGTCTATCCTCCTTTAG	144.92542
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93769_Hsan_Wil2021_COI	MC9	1	BB93769_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Absent	Sample	Hsan_Wil2021_COI	MC9	COI	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	CCTGGGCCGGTATAGTAGGT	144.92542
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93769_Msal_Bot2012_COI	MC9	1	BB93769_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Absent	Sample	Msal_Bot2012_COI	MC9	COI	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	GYTAGTTCCRATGATGTTAGCTG	144.92542
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93770_Aamu_Bax2006_COI	MC98	1	BB93770_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Absent	Sample	Aamu_Bax2006_COI	MC98	COI	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	GCACAACCGGGATCTTTACTTC	144.91328
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93770_Pcan_Bot2011_IGS	MC98	1	BB93770_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC98	IGS	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	CGTAATCCTCAGTACTGGCTA	144.91328
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.563	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93770_Sspa_Oph2007_COI	MC98	1	BB93770_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Present	Sample	Sspa_Oph2007_COI	MC98	COI	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	GAAGGCCTTCCGATGATC	144.91328
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93770_Upin_Bot2011_COI	MC98	1	BB93770_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Absent	Sample	Upin_Bot2011_COI	MC98	COI	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	CTTTAATTACAGCGTTTTTATTGTTGT	144.91328
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.993	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93771_Mgig_Bot2012_COI	MC96	1	BB93771_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Present	Sample	Mgig_Bot2012_COI	MC96	COI	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	TCTTATTCGTTGGAGACTTTATAACCCT	144.91359
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93772_Cmae_Bot2011_COI	MC94	1	BB93772_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Absent	Sample	Cmae_Bot2011_COI	MC94	COI	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	ATGAACAGTCTATCCTCCTTTAG	144.91439
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93772_Hsan_Wil2021_COI	MC94	1	BB93772_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Absent	Sample	Hsan_Wil2021_COI	MC94	COI	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	CCTGGGCCGGTATAGTAGGT	144.91439
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93772_Msal_Bot2012_COI	MC94	1	BB93772_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Absent	Sample	Msal_Bot2012_COI	MC94	COI	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	GYTAGTTCCRATGATGTTAGCTG	144.91439
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93774_Aamu_Bax2006_COI	MC89	1	BB93774_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Absent	Sample	Aamu_Bax2006_COI	MC89	COI	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	GCACAACCGGGATCTTTACTTC	144.91377
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93774_Pcan_Bot2011_IGS	MC89	1	BB93774_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC89	IGS	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	CGTAATCCTCAGTACTGGCTA	144.91377
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.337	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93774_Sspa_Oph2007_COI	MC89	1	BB93774_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Present	Sample	Sspa_Oph2007_COI	MC89	COI	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	GAAGGCCTTCCGATGATC	144.91377
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93774_Upin_Bot2011_COI	MC89	1	BB93774_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Absent	Sample	Upin_Bot2011_COI	MC89	COI	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	CTTTAATTACAGCGTTTTTATTGTTGT	144.91377
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.911	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93775_Mgig_Bot2012_COI	MC88	1	BB93775_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Present	Sample	Mgig_Bot2012_COI	MC88	COI	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	TCTTATTCGTTGGAGACTTTATAACCCT	144.91409
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93776_Cmae_Bot2011_COI	MC87	1	BB93776_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Absent	Sample	Cmae_Bot2011_COI	MC87	COI	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	ATGAACAGTCTATCCTCCTTTAG	144.92133
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93776_Hsan_Wil2021_COI	MC87	1	BB93776_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Absent	Sample	Hsan_Wil2021_COI	MC87	COI	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	CCTGGGCCGGTATAGTAGGT	144.92133
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93776_Msal_Bot2012_COI	MC87	1	BB93776_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Absent	Sample	Msal_Bot2012_COI	MC87	COI	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	GYTAGTTCCRATGATGTTAGCTG	144.92133
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93778_Aamu_Bax2006_COI	MC81	1	BB93778_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Absent	Sample	Aamu_Bax2006_COI	MC81	COI	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	GCACAACCGGGATCTTTACTTC	144.92449
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93778_Pcan_Bot2011_IGS	MC81	1	BB93778_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC81	IGS	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	CGTAATCCTCAGTACTGGCTA	144.92449
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.292	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93778_Sspa_Oph2007_COI	MC81	1	BB93778_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Present	Sample	Sspa_Oph2007_COI	MC81	COI	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	GAAGGCCTTCCGATGATC	144.92449
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93778_Upin_Bot2011_COI	MC81	1	BB93778_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Absent	Sample	Upin_Bot2011_COI	MC81	COI	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	CTTTAATTACAGCGTTTTTATTGTTGT	144.92449
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.422	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93779_Mgig_Bot2012_COI	MC7	1	BB93779_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Present	Sample	Mgig_Bot2012_COI	MC7	COI	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	TCTTATTCGTTGGAGACTTTATAACCCT	144.92731
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93781_Aamu_Bax2006_COI	MC2	1	BB93781_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Absent	Sample	Aamu_Bax2006_COI	MC2	COI	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	GCACAACCGGGATCTTTACTTC	144.93023
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93781_Pcan_Bot2011_IGS	MC2	1	BB93781_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC2	IGS	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	CGTAATCCTCAGTACTGGCTA	144.93023
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.443	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93781_Sspa_Oph2007_COI	MC2	1	BB93781_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Present	Sample	Sspa_Oph2007_COI	MC2	COI	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	GAAGGCCTTCCGATGATC	144.93023
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93781_Upin_Bot2011_COI	MC2	1	BB93781_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Absent	Sample	Upin_Bot2011_COI	MC2	COI	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	CTTTAATTACAGCGTTTTTATTGTTGT	144.93023
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.973	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93782_Mgig_Bot2012_COI	MC1	1	BB93782_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Present	Sample	Mgig_Bot2012_COI	MC1	COI	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	TCTTATTCGTTGGAGACTTTATAACCCT	144.9291
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93783_Cmae_Bot2011_COI	MC114	1	BB93783_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Absent	Sample	Cmae_Bot2011_COI	MC114	COI	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	ATGAACAGTCTATCCTCCTTTAG	144.90928
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93783_Hsan_Wil2021_COI	MC114	1	BB93783_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Absent	Sample	Hsan_Wil2021_COI	MC114	COI	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	CCTGGGCCGGTATAGTAGGT	144.90928
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93783_Msal_Bot2012_COI	MC114	1	BB93783_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Absent	Sample	Msal_Bot2012_COI	MC114	COI	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	GYTAGTTCCRATGATGTTAGCTG	144.90928
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93785_Aamu_Bax2006_COI	MC107	1	BB93785_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Absent	Sample	Aamu_Bax2006_COI	MC107	COI	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	GCACAACCGGGATCTTTACTTC	144.90595
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93785_Pcan_Bot2011_IGS	MC107	1	BB93785_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC107	IGS	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	CGTAATCCTCAGTACTGGCTA	144.90595
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.149	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93785_Sspa_Oph2007_COI	MC107	1	BB93785_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Present	Sample	Sspa_Oph2007_COI	MC107	COI	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	GAAGGCCTTCCGATGATC	144.90595
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93785_Upin_Bot2011_COI	MC107	1	BB93785_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Absent	Sample	Upin_Bot2011_COI	MC107	COI	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	CTTTAATTACAGCGTTTTTATTGTTGT	144.90595
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.938	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93786_Mgig_Bot2012_COI	MC106	1	BB93786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Present	Sample	Mgig_Bot2012_COI	MC106	COI	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	TCTTATTCGTTGGAGACTTTATAACCCT	144.91331
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93787_Cmae_Bot2011_COI	MC104	1	BB93787_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Absent	Sample	Cmae_Bot2011_COI	MC104	COI	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	ATGAACAGTCTATCCTCCTTTAG	144.91268
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93787_Hsan_Wil2021_COI	MC104	1	BB93787_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Absent	Sample	Hsan_Wil2021_COI	MC104	COI	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	CCTGGGCCGGTATAGTAGGT	144.91268
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93787_Msal_Bot2012_COI	MC104	1	BB93787_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Absent	Sample	Msal_Bot2012_COI	MC104	COI	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	GYTAGTTCCRATGATGTTAGCTG	144.91268
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93789_Aamu_Bax2006_COI	MC101	1	BB93789_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Absent	Sample	Aamu_Bax2006_COI	MC101	COI	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	GCACAACCGGGATCTTTACTTC	144.91999
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93789_Pcan_Bot2011_IGS	MC101	1	BB93789_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC101	IGS	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	CGTAATCCTCAGTACTGGCTA	144.91999
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.778	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93789_Sspa_Oph2007_COI	MC101	1	BB93789_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Present	Sample	Sspa_Oph2007_COI	MC101	COI	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	GAAGGCCTTCCGATGATC	144.91999
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93789_Upin_Bot2011_COI	MC101	1	BB93789_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Absent	Sample	Upin_Bot2011_COI	MC101	COI	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	CTTTAATTACAGCGTTTTTATTGTTGT	144.91999
BB93790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81652	WGS84	BB93790	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93790_Cmae_Bot2011_COI	MC99	1	BB93790_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93790	Sample	BB93790	targeted	Absent	Sample	Cmae_Bot2011_COI	MC99	COI	45	BB93790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC99	ATGAACAGTCTATCCTCCTTTAG	144.91313
BB93790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81652	WGS84	BB93790	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93790_Msal_Bot2012_COI	MC99	1	BB93790_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93790	Sample	BB93790	targeted	Absent	Sample	Msal_Bot2012_COI	MC99	COI	45	BB93790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC99	GYTAGTTCCRATGATGTTAGCTG	144.91313
BB93792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85883	WGS84	BB93792	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93792_Aamu_Bax2006_COI	MC124	1	BB93792_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93792	Sample	BB93792	targeted	Absent	Sample	Aamu_Bax2006_COI	MC124	COI	45	BB93792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC124	GCACAACCGGGATCTTTACTTC	144.90837
BB93792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85883	WGS84	BB93792	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93792_Pcan_Bot2011_IGS	MC124	1	BB93792_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93792	Sample	BB93792	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC124	IGS	45	BB93792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC124	CGTAATCCTCAGTACTGGCTA	144.90837
BB93792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85883	WGS84	BB93792	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.979	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93792_Sspa_Oph2007_COI	MC124	1	BB93792_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93792	Sample	BB93792	targeted	Present	Sample	Sspa_Oph2007_COI	MC124	COI	45	BB93792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC124	GAAGGCCTTCCGATGATC	144.90837
BB93792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85883	WGS84	BB93792	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.249	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93792_Upin_Bot2011_COI	MC124	1	BB93792_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93792	Sample	BB93792	targeted	Present	Sample	Upin_Bot2011_COI	MC124	COI	45	BB93792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC124	CTTTAATTACAGCGTTTTTATTGTTGT	144.90837
BB93793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8597	WGS84	BB93793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.562	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93793_Mgig_Bot2012_COI	MC123	1	BB93793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93793	Sample	BB93793	targeted	Present	Sample	Mgig_Bot2012_COI	MC123	COI	45	BB93793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC123	TCTTATTCGTTGGAGACTTTATAACCCT	144.90663
BB93794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82012	WGS84	BB93794	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93794_Cmae_Bot2011_COI	MC119	1	BB93794_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93794	Sample	BB93794	targeted	Absent	Sample	Cmae_Bot2011_COI	MC119	COI	45	BB93794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC119	ATGAACAGTCTATCCTCCTTTAG	144.90604
BB93794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82012	WGS84	BB93794	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93794_Msal_Bot2012_COI	MC119	1	BB93794_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93794	Sample	BB93794	targeted	Absent	Sample	Msal_Bot2012_COI	MC119	COI	45	BB93794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC119	GYTAGTTCCRATGATGTTAGCTG	144.90604
BB93796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82232	WGS84	BB93796	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.997	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93796_Aamu_Bax2006_COI	MC117	1	BB93796_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93796	Sample	BB93796	targeted	Present	Sample	Aamu_Bax2006_COI	MC117	COI	45	BB93796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC117	GCACAACCGGGATCTTTACTTC	144.90786
BB93796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82232	WGS84	BB93796	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93796_Pcan_Bot2011_IGS	MC117	1	BB93796_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93796	Sample	BB93796	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC117	IGS	45	BB93796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC117	CGTAATCCTCAGTACTGGCTA	144.90786
BB93796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82232	WGS84	BB93796	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.578	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93796_Sspa_Oph2007_COI	MC117	1	BB93796_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93796	Sample	BB93796	targeted	Present	Sample	Sspa_Oph2007_COI	MC117	COI	45	BB93796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC117	GAAGGCCTTCCGATGATC	144.90786
BB93796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82232	WGS84	BB93796	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93796_Upin_Bot2011_COI	MC117	1	BB93796_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93796	Sample	BB93796	targeted	Absent	Sample	Upin_Bot2011_COI	MC117	COI	45	BB93796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC117	CTTTAATTACAGCGTTTTTATTGTTGT	144.90786
BB93802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4292	WGS84	BB93802	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93802_Cmae_Bot2011_COI	BC45	1	BB93802_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93802	Sample	BB93802	targeted	Absent	Sample	Cmae_Bot2011_COI	BC45	COI	45	BB93802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC45	ATGAACAGTCTATCCTCCTTTAG	153.12194
BB93802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4292	WGS84	BB93802	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93802_Msal_Bot2012_COI	BC45	1	BB93802_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93802	Sample	BB93802	targeted	Absent	Sample	Msal_Bot2012_COI	BC45	COI	45	BB93802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC45	GYTAGTTCCRATGATGTTAGCTG	153.12194
BB93804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42155	WGS84	BB93804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93804_Aamu_Bax2006_COI	BC35	1	BB93804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93804	Sample	BB93804	targeted	Absent	Sample	Aamu_Bax2006_COI	BC35	COI	45	BB93804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC35	GCACAACCGGGATCTTTACTTC	153.13604
BB93804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42155	WGS84	BB93804	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93804_Pcan_Bot2011_IGS	BC35	1	BB93804_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93804	Sample	BB93804	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC35	IGS	45	BB93804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC35	CGTAATCCTCAGTACTGGCTA	153.13604
BB93804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42155	WGS84	BB93804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93804_Sspa_Oph2007_COI	BC35	1	BB93804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93804	Sample	BB93804	targeted	Absent	Sample	Sspa_Oph2007_COI	BC35	COI	45	BB93804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC35	GAAGGCCTTCCGATGATC	153.13604
BB93804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42155	WGS84	BB93804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93804_Upin_Bot2011_COI	BC35	1	BB93804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93804	Sample	BB93804	targeted	Absent	Sample	Upin_Bot2011_COI	BC35	COI	45	BB93804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC35	CTTTAATTACAGCGTTTTTATTGTTGT	153.13604
BB93805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44585	WGS84	BB93805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93805_Mgig_Bot2012_COI	BC65	1	BB93805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93805	Sample	BB93805	targeted	Absent	Sample	Mgig_Bot2012_COI	BC65	COI	45	BB93805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC65	TCTTATTCGTTGGAGACTTTATAACCCT	153.08404
BB93806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44408	WGS84	BB93806	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93806_Cmae_Bot2011_COI	BC55	1	BB93806_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93806	Sample	BB93806	targeted	Absent	Sample	Cmae_Bot2011_COI	BC55	COI	45	BB93806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC55	ATGAACAGTCTATCCTCCTTTAG	153.07674
BB93806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44408	WGS84	BB93806	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93806_Msal_Bot2012_COI	BC55	1	BB93806_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93806	Sample	BB93806	targeted	Absent	Sample	Msal_Bot2012_COI	BC55	COI	45	BB93806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC55	GYTAGTTCCRATGATGTTAGCTG	153.07674
BB93808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38487	WGS84	BB93808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93808_Aamu_Bax2006_COI	BC20	1	BB93808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93808	Sample	BB93808	targeted	Absent	Sample	Aamu_Bax2006_COI	BC20	COI	45	BB93808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC20	GCACAACCGGGATCTTTACTTC	153.15971
BB93808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38487	WGS84	BB93808	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93808_Pcan_Bot2011_IGS	BC20	1	BB93808_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93808	Sample	BB93808	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC20	IGS	45	BB93808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC20	CGTAATCCTCAGTACTGGCTA	153.15971
BB93808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38487	WGS84	BB93808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93808_Sspa_Oph2007_COI	BC20	1	BB93808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93808	Sample	BB93808	targeted	Absent	Sample	Sspa_Oph2007_COI	BC20	COI	45	BB93808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC20	GAAGGCCTTCCGATGATC	153.15971
BB93808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38487	WGS84	BB93808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93808_Upin_Bot2011_COI	BC20	1	BB93808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93808	Sample	BB93808	targeted	Absent	Sample	Upin_Bot2011_COI	BC20	COI	45	BB93808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC20	CTTTAATTACAGCGTTTTTATTGTTGT	153.15971
BB93809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37857	WGS84	BB93809	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93809_Mgig_Bot2012_COI	BC15	1	BB93809_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93809	Sample	BB93809	targeted	Absent	Sample	Mgig_Bot2012_COI	BC15	COI	45	BB93809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC15	TCTTATTCGTTGGAGACTTTATAACCCT	153.1651
BB93811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43258	WGS84	BB93811	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93811_Aamu_Bax2006_COI	BC48	1	BB93811_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93811	Sample	BB93811	targeted	Absent	Sample	Aamu_Bax2006_COI	BC48	COI	45	BB93811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC48	GCACAACCGGGATCTTTACTTC	153.11706
BB93811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43258	WGS84	BB93811	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93811_Pcan_Bot2011_IGS	BC48	1	BB93811_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93811	Sample	BB93811	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC48	IGS	45	BB93811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC48	CGTAATCCTCAGTACTGGCTA	153.11706
BB93811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43258	WGS84	BB93811	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93811_Sspa_Oph2007_COI	BC48	1	BB93811_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93811	Sample	BB93811	targeted	Absent	Sample	Sspa_Oph2007_COI	BC48	COI	45	BB93811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC48	GAAGGCCTTCCGATGATC	153.11706
BB93811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43258	WGS84	BB93811	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93811_Upin_Bot2011_COI	BC48	1	BB93811_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93811	Sample	BB93811	targeted	Absent	Sample	Upin_Bot2011_COI	BC48	COI	45	BB93811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC48	CTTTAATTACAGCGTTTTTATTGTTGT	153.11706
BB93812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43069	WGS84	BB93812	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93812_Mgig_Bot2012_COI	BC47	1	BB93812_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93812	Sample	BB93812	targeted	Absent	Sample	Mgig_Bot2012_COI	BC47	COI	45	BB93812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC47	TCTTATTCGTTGGAGACTTTATAACCCT	153.11845
BB93813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42924	WGS84	BB93813	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93813_Cmae_Bot2011_COI	BC41	1	BB93813_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93813	Sample	BB93813	targeted	Absent	Sample	Cmae_Bot2011_COI	BC41	COI	45	BB93813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC41	ATGAACAGTCTATCCTCCTTTAG	153.1243
BB93813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42924	WGS84	BB93813	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93813_Msal_Bot2012_COI	BC41	1	BB93813_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93813	Sample	BB93813	targeted	Absent	Sample	Msal_Bot2012_COI	BC41	COI	45	BB93813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC41	GYTAGTTCCRATGATGTTAGCTG	153.1243
BB93815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.41408	WGS84	BB93815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93815_Aamu_Bax2006_COI	BC34	1	BB93815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93815	Sample	BB93815	targeted	Absent	Sample	Aamu_Bax2006_COI	BC34	COI	45	BB93815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC34	GCACAACCGGGATCTTTACTTC	153.14362
BB93815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.41408	WGS84	BB93815	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93815_Pcan_Bot2011_IGS	BC34	1	BB93815_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93815	Sample	BB93815	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC34	IGS	45	BB93815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC34	CGTAATCCTCAGTACTGGCTA	153.14362
BB93815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.41408	WGS84	BB93815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93815_Sspa_Oph2007_COI	BC34	1	BB93815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93815	Sample	BB93815	targeted	Absent	Sample	Sspa_Oph2007_COI	BC34	COI	45	BB93815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC34	GAAGGCCTTCCGATGATC	153.14362
BB93815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.41408	WGS84	BB93815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93815_Upin_Bot2011_COI	BC34	1	BB93815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93815	Sample	BB93815	targeted	Absent	Sample	Upin_Bot2011_COI	BC34	COI	45	BB93815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC34	CTTTAATTACAGCGTTTTTATTGTTGT	153.14362
BB93816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40652	WGS84	BB93816	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93816_Mgig_Bot2012_COI	BC33	1	BB93816_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93816	Sample	BB93816	targeted	Absent	Sample	Mgig_Bot2012_COI	BC33	COI	45	BB93816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC33	TCTTATTCGTTGGAGACTTTATAACCCT	153.15089
BB93817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4041	WGS84	BB93817	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93817_Cmae_Bot2011_COI	BC31	1	BB93817_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93817	Sample	BB93817	targeted	Absent	Sample	Cmae_Bot2011_COI	BC31	COI	45	BB93817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC31	ATGAACAGTCTATCCTCCTTTAG	153.15312
BB93817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4041	WGS84	BB93817	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93817_Msal_Bot2012_COI	BC31	1	BB93817_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93817	Sample	BB93817	targeted	Absent	Sample	Msal_Bot2012_COI	BC31	COI	45	BB93817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC31	GYTAGTTCCRATGATGTTAGCTG	153.15312
BB93819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40095	WGS84	BB93819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93819_Aamu_Bax2006_COI	BC28	1	BB93819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93819	Sample	BB93819	targeted	Absent	Sample	Aamu_Bax2006_COI	BC28	COI	45	BB93819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC28	GCACAACCGGGATCTTTACTTC	153.1607
BB93819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40095	WGS84	BB93819	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93819_Pcan_Bot2011_IGS	BC28	1	BB93819_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93819	Sample	BB93819	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC28	IGS	45	BB93819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC28	CGTAATCCTCAGTACTGGCTA	153.1607
BB93819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40095	WGS84	BB93819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93819_Sspa_Oph2007_COI	BC28	1	BB93819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93819	Sample	BB93819	targeted	Absent	Sample	Sspa_Oph2007_COI	BC28	COI	45	BB93819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC28	GAAGGCCTTCCGATGATC	153.1607
BB93819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40095	WGS84	BB93819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93819_Upin_Bot2011_COI	BC28	1	BB93819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93819	Sample	BB93819	targeted	Absent	Sample	Upin_Bot2011_COI	BC28	COI	45	BB93819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC28	CTTTAATTACAGCGTTTTTATTGTTGT	153.1607
BB93820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40096	WGS84	BB93820	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93820_Cmae_Bot2011_COI	BC26	1	BB93820_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93820	Sample	BB93820	targeted	Absent	Sample	Cmae_Bot2011_COI	BC26	COI	45	BB93820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC26	ATGAACAGTCTATCCTCCTTTAG	153.16291
BB93820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40096	WGS84	BB93820	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93820_Msal_Bot2012_COI	BC26	1	BB93820_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93820	Sample	BB93820	targeted	Absent	Sample	Msal_Bot2012_COI	BC26	COI	45	BB93820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC26	GYTAGTTCCRATGATGTTAGCTG	153.16291
BB93822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44499	WGS84	BB93822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93822_Aamu_Bax2006_COI	BC61	1	BB93822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93822	Sample	BB93822	targeted	Absent	Sample	Aamu_Bax2006_COI	BC61	COI	45	BB93822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC61	GCACAACCGGGATCTTTACTTC	153.08037
BB93822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44499	WGS84	BB93822	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93822_Pcan_Bot2011_IGS	BC61	1	BB93822_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93822	Sample	BB93822	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC61	IGS	45	BB93822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC61	CGTAATCCTCAGTACTGGCTA	153.08037
BB93822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44499	WGS84	BB93822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93822_Sspa_Oph2007_COI	BC61	1	BB93822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93822	Sample	BB93822	targeted	Absent	Sample	Sspa_Oph2007_COI	BC61	COI	45	BB93822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC61	GAAGGCCTTCCGATGATC	153.08037
BB93822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44499	WGS84	BB93822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93822_Upin_Bot2011_COI	BC61	1	BB93822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93822	Sample	BB93822	targeted	Absent	Sample	Upin_Bot2011_COI	BC61	COI	45	BB93822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC61	CTTTAATTACAGCGTTTTTATTGTTGT	153.08037
BB93823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44525	WGS84	BB93823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93823_Mgig_Bot2012_COI	BC59	1	BB93823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93823	Sample	BB93823	targeted	Absent	Sample	Mgig_Bot2012_COI	BC59	COI	45	BB93823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC59	TCTTATTCGTTGGAGACTTTATAACCCT	153.07854
BB93824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44527	WGS84	BB93824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93824_Cmae_Bot2011_COI	BC56	1	BB93824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93824	Sample	BB93824	targeted	Absent	Sample	Cmae_Bot2011_COI	BC56	COI	45	BB93824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC56	ATGAACAGTCTATCCTCCTTTAG	153.07559
BB93824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44527	WGS84	BB93824	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93824_Msal_Bot2012_COI	BC56	1	BB93824_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93824	Sample	BB93824	targeted	Absent	Sample	Msal_Bot2012_COI	BC56	COI	45	BB93824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC56	GYTAGTTCCRATGATGTTAGCTG	153.07559
BB93826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44257	WGS84	BB93826	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93826_Aamu_Bax2006_COI	BC52	1	BB93826_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93826	Sample	BB93826	targeted	Absent	Sample	Aamu_Bax2006_COI	BC52	COI	45	BB93826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC52	GCACAACCGGGATCTTTACTTC	153.07162
BB93826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44257	WGS84	BB93826	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93826_Pcan_Bot2011_IGS	BC52	1	BB93826_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93826	Sample	BB93826	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC52	IGS	45	BB93826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC52	CGTAATCCTCAGTACTGGCTA	153.07162
BB93826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44257	WGS84	BB93826	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93826_Sspa_Oph2007_COI	BC52	1	BB93826_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93826	Sample	BB93826	targeted	Absent	Sample	Sspa_Oph2007_COI	BC52	COI	45	BB93826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC52	GAAGGCCTTCCGATGATC	153.07162
BB93826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44257	WGS84	BB93826	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93826_Upin_Bot2011_COI	BC52	1	BB93826_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93826	Sample	BB93826	targeted	Absent	Sample	Upin_Bot2011_COI	BC52	COI	45	BB93826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC52	CTTTAATTACAGCGTTTTTATTGTTGT	153.07162
BB93827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36951	WGS84	BB93827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93827_Mgig_Bot2012_COI	BC9	1	BB93827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93827	Sample	BB93827	targeted	Absent	Sample	Mgig_Bot2012_COI	BC9	COI	45	BB93827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC9	TCTTATTCGTTGGAGACTTTATAACCCT	153.17173
BB93828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36653	WGS84	BB93828	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93828_Cmae_Bot2011_COI	BC7	1	BB93828_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93828	Sample	BB93828	targeted	Absent	Sample	Cmae_Bot2011_COI	BC7	COI	45	BB93828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC7	ATGAACAGTCTATCCTCCTTTAG	153.17384
BB93828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36653	WGS84	BB93828	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93828_Msal_Bot2012_COI	BC7	1	BB93828_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93828	Sample	BB93828	targeted	Absent	Sample	Msal_Bot2012_COI	BC7	COI	45	BB93828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC7	GYTAGTTCCRATGATGTTAGCTG	153.17384
BB93830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35982	WGS84	BB93830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93830_Mgig_Bot2012_COI	BC3	1	BB93830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93830	Sample	BB93830	targeted	Absent	Sample	Mgig_Bot2012_COI	BC3	COI	45	BB93830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC3	TCTTATTCGTTGGAGACTTTATAACCCT	153.17885
BB93831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3909	WGS84	BB93831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93831_Cmae_Bot2011_COI	BC24	1	BB93831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93831	Sample	BB93831	targeted	Absent	Sample	Cmae_Bot2011_COI	BC24	COI	45	BB93831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC24	ATGAACAGTCTATCCTCCTTTAG	153.15723
BB93831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3909	WGS84	BB93831	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93831_Msal_Bot2012_COI	BC24	1	BB93831_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93831	Sample	BB93831	targeted	Absent	Sample	Msal_Bot2012_COI	BC24	COI	45	BB93831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC24	GYTAGTTCCRATGATGTTAGCTG	153.15723
BB93833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38259	WGS84	BB93833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93833_Aamu_Bax2006_COI	BC18	1	BB93833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93833	Sample	BB93833	targeted	Absent	Sample	Aamu_Bax2006_COI	BC18	COI	45	BB93833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC18	GCACAACCGGGATCTTTACTTC	153.16112
BB93833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38259	WGS84	BB93833	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93833_Pcan_Bot2011_IGS	BC18	1	BB93833_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93833	Sample	BB93833	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC18	IGS	45	BB93833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC18	CGTAATCCTCAGTACTGGCTA	153.16112
BB93833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38259	WGS84	BB93833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93833_Sspa_Oph2007_COI	BC18	1	BB93833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93833	Sample	BB93833	targeted	Absent	Sample	Sspa_Oph2007_COI	BC18	COI	45	BB93833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC18	GAAGGCCTTCCGATGATC	153.16112
BB93833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38259	WGS84	BB93833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93833_Upin_Bot2011_COI	BC18	1	BB93833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93833	Sample	BB93833	targeted	Absent	Sample	Upin_Bot2011_COI	BC18	COI	45	BB93833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC18	CTTTAATTACAGCGTTTTTATTGTTGT	153.16112
BB93834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38037	WGS84	BB93834	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93834_Mgig_Bot2012_COI	BC17	1	BB93834_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93834	Sample	BB93834	targeted	Absent	Sample	Mgig_Bot2012_COI	BC17	COI	45	BB93834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC17	TCTTATTCGTTGGAGACTTTATAACCCT	153.16371
BB93835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37563	WGS84	BB93835	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93835_Cmae_Bot2011_COI	BC13	1	BB93835_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93835	Sample	BB93835	targeted	Absent	Sample	Cmae_Bot2011_COI	BC13	COI	45	BB93835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC13	ATGAACAGTCTATCCTCCTTTAG	153.16734
BB93835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37563	WGS84	BB93835	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93835_Msal_Bot2012_COI	BC13	1	BB93835_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93835	Sample	BB93835	targeted	Absent	Sample	Msal_Bot2012_COI	BC13	COI	45	BB93835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC13	GYTAGTTCCRATGATGTTAGCTG	153.16734
BB93837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35649	WGS84	BB93837	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93837_Aamu_Bax2006_COI	BC1	1	BB93837_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93837	Sample	BB93837	targeted	Absent	Sample	Aamu_Bax2006_COI	BC1	COI	45	BB93837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC1	GCACAACCGGGATCTTTACTTC	153.18075
BB93837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35649	WGS84	BB93837	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93837_Pcan_Bot2011_IGS	BC1	1	BB93837_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93837	Sample	BB93837	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC1	IGS	45	BB93837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC1	CGTAATCCTCAGTACTGGCTA	153.18075
BB93837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35649	WGS84	BB93837	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93837_Sspa_Oph2007_COI	BC1	1	BB93837_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93837	Sample	BB93837	targeted	Absent	Sample	Sspa_Oph2007_COI	BC1	COI	45	BB93837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC1	GAAGGCCTTCCGATGATC	153.18075
BB93837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35649	WGS84	BB93837	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93837_Upin_Bot2011_COI	BC1	1	BB93837_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93837	Sample	BB93837	targeted	Absent	Sample	Upin_Bot2011_COI	BC1	COI	45	BB93837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC1	CTTTAATTACAGCGTTTTTATTGTTGT	153.18075
BB93838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44809	WGS84	BB93838	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93838_Mgig_Bot2012_COI	BC51	1	BB93838_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93838	Sample	BB93838	targeted	Absent	Sample	Mgig_Bot2012_COI	BC51	COI	45	BB93838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC51	TCTTATTCGTTGGAGACTTTATAACCCT	153.09371
BB93855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.844	WGS84	BB93855	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93855_Aamu_Bax2006_COI	HC5	1	BB93855_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93855	Sample	BB93855	targeted	Absent	Sample	Aamu_Bax2006_COI	HC5	COI	45	BB93855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC5	GCACAACCGGGATCTTTACTTC	147.3277
BB93855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.844	WGS84	BB93855	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93855_Pcan_Bot2011_IGS	HC5	1	BB93855_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93855	Sample	BB93855	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC5	IGS	45	BB93855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC5	CGTAATCCTCAGTACTGGCTA	147.3277
BB93855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.844	WGS84	BB93855	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93855_Sspa_Oph2007_COI	HC5	1	BB93855_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93855	Sample	BB93855	targeted	Absent	Sample	Sspa_Oph2007_COI	HC5	COI	45	BB93855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC5	GAAGGCCTTCCGATGATC	147.3277
BB93855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.844	WGS84	BB93855	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93855_Upin_Bot2011_COI	HC5	1	BB93855_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93855	Sample	BB93855	targeted	Absent	Sample	Upin_Bot2011_COI	HC5	COI	45	BB93855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC5	CTTTAATTACAGCGTTTTTATTGTTGT	147.3277
BB93856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8745	WGS84	BB93856	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.771	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93856_Mgig_Bot2012_COI	HC23	1	BB93856_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93856	Sample	BB93856	targeted	Present	Sample	Mgig_Bot2012_COI	HC23	COI	45	BB93856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC23	TCTTATTCGTTGGAGACTTTATAACCCT	147.3381
BB93857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.875	WGS84	BB93857	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93857_Cmae_Bot2011_COI	HC22	1	BB93857_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93857	Sample	BB93857	targeted	Absent	Sample	Cmae_Bot2011_COI	HC22	COI	45	BB93857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC22	ATGAACAGTCTATCCTCCTTTAG	147.3394
BB93857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.875	WGS84	BB93857	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93857_Msal_Bot2012_COI	HC22	1	BB93857_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93857	Sample	BB93857	targeted	Absent	Sample	Msal_Bot2012_COI	HC22	COI	45	BB93857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC22	GYTAGTTCCRATGATGTTAGCTG	147.3394
BB93859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8818	WGS84	BB93859	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93859_Aamu_Bax2006_COI	HC17	1	BB93859_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93859	Sample	BB93859	targeted	Absent	Sample	Aamu_Bax2006_COI	HC17	COI	45	BB93859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC17	GCACAACCGGGATCTTTACTTC	147.3426
BB93859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8818	WGS84	BB93859	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93859_Pcan_Bot2011_IGS	HC17	1	BB93859_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93859	Sample	BB93859	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC17	IGS	45	BB93859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC17	CGTAATCCTCAGTACTGGCTA	147.3426
BB93859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8818	WGS84	BB93859	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93859_Sspa_Oph2007_COI	HC17	1	BB93859_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93859	Sample	BB93859	targeted	Absent	Sample	Sspa_Oph2007_COI	HC17	COI	45	BB93859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC17	GAAGGCCTTCCGATGATC	147.3426
BB93859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8818	WGS84	BB93859	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93859_Upin_Bot2011_COI	HC17	1	BB93859_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93859	Sample	BB93859	targeted	Absent	Sample	Upin_Bot2011_COI	HC17	COI	45	BB93859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC17	CTTTAATTACAGCGTTTTTATTGTTGT	147.3426
BB93860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8829	WGS84	BB93860	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93860_Cmae_Bot2011_COI	HC16	1	BB93860_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93860	Sample	BB93860	targeted	Absent	Sample	Cmae_Bot2011_COI	HC16	COI	45	BB93860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC16	ATGAACAGTCTATCCTCCTTTAG	147.3419
BB93860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8829	WGS84	BB93860	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93860_Msal_Bot2012_COI	HC16	1	BB93860_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93860	Sample	BB93860	targeted	Absent	Sample	Msal_Bot2012_COI	HC16	COI	45	BB93860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC16	GYTAGTTCCRATGATGTTAGCTG	147.3419
BB93862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8828	WGS84	BB93862	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93862_Aamu_Bax2006_COI	HC13	1	BB93862_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93862	Sample	BB93862	targeted	Absent	Sample	Aamu_Bax2006_COI	HC13	COI	45	BB93862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC13	GCACAACCGGGATCTTTACTTC	147.3363
BB93862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8828	WGS84	BB93862	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93862_Pcan_Bot2011_IGS	HC13	1	BB93862_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93862	Sample	BB93862	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC13	IGS	45	BB93862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC13	CGTAATCCTCAGTACTGGCTA	147.3363
BB93862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8828	WGS84	BB93862	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93862_Sspa_Oph2007_COI	HC13	1	BB93862_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93862	Sample	BB93862	targeted	Absent	Sample	Sspa_Oph2007_COI	HC13	COI	45	BB93862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC13	GAAGGCCTTCCGATGATC	147.3363
BB93862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8828	WGS84	BB93862	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93862_Upin_Bot2011_COI	HC13	1	BB93862_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93862	Sample	BB93862	targeted	Absent	Sample	Upin_Bot2011_COI	HC13	COI	45	BB93862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC13	CTTTAATTACAGCGTTTTTATTGTTGT	147.3363
BB93863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8485	WGS84	BB93863	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.772	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93863_Mgig_Bot2012_COI	HC11	1	BB93863_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93863	Sample	BB93863	targeted	Present	Sample	Mgig_Bot2012_COI	HC11	COI	45	BB93863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC11	TCTTATTCGTTGGAGACTTTATAACCCT	147.3283
BB93864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8461	WGS84	BB93864	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93864_Cmae_Bot2011_COI	HC9	1	BB93864_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93864	Sample	BB93864	targeted	Absent	Sample	Cmae_Bot2011_COI	HC9	COI	45	BB93864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC9	ATGAACAGTCTATCCTCCTTTAG	147.3297
BB93864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8461	WGS84	BB93864	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93864_Msal_Bot2012_COI	HC9	1	BB93864_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93864	Sample	BB93864	targeted	Absent	Sample	Msal_Bot2012_COI	HC9	COI	45	BB93864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC9	GYTAGTTCCRATGATGTTAGCTG	147.3297
BB93866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93866	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93866_Aamu_Bax2006_COI	HC4	1	BB93866_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93866	Sample	BB93866	targeted	Absent	Sample	Aamu_Bax2006_COI	HC4	COI	45	BB93866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC4	GCACAACCGGGATCTTTACTTC	147.3295
BB93866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93866	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93866_Pcan_Bot2011_IGS	HC4	1	BB93866_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93866	Sample	BB93866	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC4	IGS	45	BB93866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC4	CGTAATCCTCAGTACTGGCTA	147.3295
BB93866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93866	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93866_Sspa_Oph2007_COI	HC4	1	BB93866_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93866	Sample	BB93866	targeted	Absent	Sample	Sspa_Oph2007_COI	HC4	COI	45	BB93866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC4	GAAGGCCTTCCGATGATC	147.3295
BB93866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93866	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93866_Upin_Bot2011_COI	HC4	1	BB93866_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93866	Sample	BB93866	targeted	Absent	Sample	Upin_Bot2011_COI	HC4	COI	45	BB93866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC4	CTTTAATTACAGCGTTTTTATTGTTGT	147.3295
BB93867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8284	WGS84	BB93867	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.557	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93867_Mgig_Bot2012_COI	HC3	1	BB93867_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93867	Sample	BB93867	targeted	Present	Sample	Mgig_Bot2012_COI	HC3	COI	45	BB93867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC3	TCTTATTCGTTGGAGACTTTATAACCCT	147.3166
BB93868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.831	WGS84	BB93868	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93868_Cmae_Bot2011_COI	HC1	1	BB93868_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93868	Sample	BB93868	targeted	Absent	Sample	Cmae_Bot2011_COI	HC1	COI	45	BB93868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC1	ATGAACAGTCTATCCTCCTTTAG	147.3215
BB93868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.831	WGS84	BB93868	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93868_Msal_Bot2012_COI	HC1	1	BB93868_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93868	Sample	BB93868	targeted	Absent	Sample	Msal_Bot2012_COI	HC1	COI	45	BB93868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC1	GYTAGTTCCRATGATGTTAGCTG	147.3215
BB93874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8071	WGS84	BB93874	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93874_Mgig_Bot2012_COI	GC110	1	BB93874_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93874	Sample	BB93874	targeted	Absent	Sample	Mgig_Bot2012_COI	GC110	COI	45	BB93874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC110	TCTTATTCGTTGGAGACTTTATAACCCT	151.21783
BB93875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80585	WGS84	BB93875	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93875_Cmae_Bot2011_COI	GC105	1	BB93875_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93875	Sample	BB93875	targeted	Absent	Sample	Cmae_Bot2011_COI	GC105	COI	45	BB93875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC105	ATGAACAGTCTATCCTCCTTTAG	151.21913
BB93875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80585	WGS84	BB93875	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93875_Msal_Bot2012_COI	GC105	1	BB93875_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93875	Sample	BB93875	targeted	Absent	Sample	Msal_Bot2012_COI	GC105	COI	45	BB93875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC105	GYTAGTTCCRATGATGTTAGCTG	151.21913
BB93877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85248	WGS84	BB93877	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93877_Aamu_Bax2006_COI	GC5	1	BB93877_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93877	Sample	BB93877	targeted	Absent	Sample	Aamu_Bax2006_COI	GC5	COI	45	BB93877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC5	GCACAACCGGGATCTTTACTTC	151.31446
BB93877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85248	WGS84	BB93877	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93877_Pcan_Bot2011_IGS	GC5	1	BB93877_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93877	Sample	BB93877	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC5	IGS	45	BB93877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC5	CGTAATCCTCAGTACTGGCTA	151.31446
BB93877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85248	WGS84	BB93877	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93877_Sspa_Oph2007_COI	GC5	1	BB93877_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93877	Sample	BB93877	targeted	Absent	Sample	Sspa_Oph2007_COI	GC5	COI	45	BB93877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC5	GAAGGCCTTCCGATGATC	151.31446
BB93877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85248	WGS84	BB93877	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93877_Upin_Bot2011_COI	GC5	1	BB93877_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93877	Sample	BB93877	targeted	Absent	Sample	Upin_Bot2011_COI	GC5	COI	45	BB93877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC5	CTTTAATTACAGCGTTTTTATTGTTGT	151.31446
BB93878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79136	WGS84	BB93878	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93878_Mgig_Bot2012_COI	GC100	1	BB93878_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93878	Sample	BB93878	targeted	Absent	Sample	Mgig_Bot2012_COI	GC100	COI	45	BB93878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC100	TCTTATTCGTTGGAGACTTTATAACCCT	151.17803
BB93879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78941	WGS84	BB93879	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93879_Cmae_Bot2011_COI	GC95	1	BB93879_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93879	Sample	BB93879	targeted	Absent	Sample	Cmae_Bot2011_COI	GC95	COI	45	BB93879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC95	ATGAACAGTCTATCCTCCTTTAG	151.18013
BB93879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78941	WGS84	BB93879	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93879_Msal_Bot2012_COI	GC95	1	BB93879_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93879	Sample	BB93879	targeted	Absent	Sample	Msal_Bot2012_COI	GC95	COI	45	BB93879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC95	GYTAGTTCCRATGATGTTAGCTG	151.18013
BB93880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78768	WGS84	BB93880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93880_Aamu_Bax2006_COI	GC90	1	BB93880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93880	Sample	BB93880	targeted	Absent	Sample	Aamu_Bax2006_COI	GC90	COI	45	BB93880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC90	GCACAACCGGGATCTTTACTTC	151.17541
BB93880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78768	WGS84	BB93880	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93880_Pcan_Bot2011_IGS	GC90	1	BB93880_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93880	Sample	BB93880	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC90	IGS	45	BB93880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC90	CGTAATCCTCAGTACTGGCTA	151.17541
BB93880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78768	WGS84	BB93880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93880_Sspa_Oph2007_COI	GC90	1	BB93880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93880	Sample	BB93880	targeted	Absent	Sample	Sspa_Oph2007_COI	GC90	COI	45	BB93880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC90	GAAGGCCTTCCGATGATC	151.17541
BB93880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78768	WGS84	BB93880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93880_Upin_Bot2011_COI	GC90	1	BB93880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93880	Sample	BB93880	targeted	Absent	Sample	Upin_Bot2011_COI	GC90	COI	45	BB93880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC90	CTTTAATTACAGCGTTTTTATTGTTGT	151.17541
BB93881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7864	WGS84	BB93881	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93881_Mgig_Bot2012_COI	GC85	1	BB93881_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93881	Sample	BB93881	targeted	Absent	Sample	Mgig_Bot2012_COI	GC85	COI	45	BB93881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC85	TCTTATTCGTTGGAGACTTTATAACCCT	151.17264
BB93882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78499	WGS84	BB93882	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93882_Cmae_Bot2011_COI	GC80	1	BB93882_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93882	Sample	BB93882	targeted	Absent	Sample	Cmae_Bot2011_COI	GC80	COI	45	BB93882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC80	ATGAACAGTCTATCCTCCTTTAG	151.20342
BB93882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78499	WGS84	BB93882	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93882_Msal_Bot2012_COI	GC80	1	BB93882_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93882	Sample	BB93882	targeted	Absent	Sample	Msal_Bot2012_COI	GC80	COI	45	BB93882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC80	GYTAGTTCCRATGATGTTAGCTG	151.20342
BB93884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81554	WGS84	BB93884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93884_Aamu_Bax2006_COI	GC65	1	BB93884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93884	Sample	BB93884	targeted	Absent	Sample	Aamu_Bax2006_COI	GC65	COI	45	BB93884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC65	GCACAACCGGGATCTTTACTTC	151.23926
BB93884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81554	WGS84	BB93884	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93884_Pcan_Bot2011_IGS	GC65	1	BB93884_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93884	Sample	BB93884	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC65	IGS	45	BB93884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC65	CGTAATCCTCAGTACTGGCTA	151.23926
BB93884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81554	WGS84	BB93884	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93884_Sspa_Oph2007_COI	GC65	1	BB93884_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93884	Sample	BB93884	targeted	Absent	Sample	Sspa_Oph2007_COI	GC65	COI	45	BB93884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC65	GAAGGCCTTCCGATGATC	151.23926
BB93884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81554	WGS84	BB93884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93884_Upin_Bot2011_COI	GC65	1	BB93884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93884	Sample	BB93884	targeted	Absent	Sample	Upin_Bot2011_COI	GC65	COI	45	BB93884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC65	CTTTAATTACAGCGTTTTTATTGTTGT	151.23926
BB93885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82641	WGS84	BB93885	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93885_Mgig_Bot2012_COI	GC60	1	BB93885_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93885	Sample	BB93885	targeted	Absent	Sample	Mgig_Bot2012_COI	GC60	COI	45	BB93885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC60	TCTTATTCGTTGGAGACTTTATAACCCT	151.24732
BB93886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8227	WGS84	BB93886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93886_Cmae_Bot2011_COI	GC55	1	BB93886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93886	Sample	BB93886	targeted	Absent	Sample	Cmae_Bot2011_COI	GC55	COI	45	BB93886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC55	ATGAACAGTCTATCCTCCTTTAG	151.24297
BB93886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8227	WGS84	BB93886	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93886_Msal_Bot2012_COI	GC55	1	BB93886_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93886	Sample	BB93886	targeted	Absent	Sample	Msal_Bot2012_COI	GC55	COI	45	BB93886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC55	GYTAGTTCCRATGATGTTAGCTG	151.24297
BB93888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82403	WGS84	BB93888	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93888_Aamu_Bax2006_COI	GC40	1	BB93888_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93888	Sample	BB93888	targeted	Absent	Sample	Aamu_Bax2006_COI	GC40	COI	45	BB93888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC40	GCACAACCGGGATCTTTACTTC	151.2451
BB93888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82403	WGS84	BB93888	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93888_Pcan_Bot2011_IGS	GC40	1	BB93888_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93888	Sample	BB93888	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC40	IGS	45	BB93888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC40	CGTAATCCTCAGTACTGGCTA	151.2451
BB93888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82403	WGS84	BB93888	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93888_Sspa_Oph2007_COI	GC40	1	BB93888_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93888	Sample	BB93888	targeted	Absent	Sample	Sspa_Oph2007_COI	GC40	COI	45	BB93888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC40	GAAGGCCTTCCGATGATC	151.2451
BB93888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82403	WGS84	BB93888	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93888_Upin_Bot2011_COI	GC40	1	BB93888_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93888	Sample	BB93888	targeted	Absent	Sample	Upin_Bot2011_COI	GC40	COI	45	BB93888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC40	CTTTAATTACAGCGTTTTTATTGTTGT	151.2451
BB93889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83417	WGS84	BB93889	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93889_Mgig_Bot2012_COI	GC35	1	BB93889_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93889	Sample	BB93889	targeted	Absent	Sample	Mgig_Bot2012_COI	GC35	COI	45	BB93889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC35	TCTTATTCGTTGGAGACTTTATAACCCT	151.2657
BB93891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8081	WGS84	BB93891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93891_Aamu_Bax2006_COI	GC107	1	BB93891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93891	Sample	BB93891	targeted	Absent	Sample	Aamu_Bax2006_COI	GC107	COI	45	BB93891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC107	GCACAACCGGGATCTTTACTTC	151.22028
BB93891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8081	WGS84	BB93891	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93891_Pcan_Bot2011_IGS	GC107	1	BB93891_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93891	Sample	BB93891	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC107	IGS	45	BB93891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC107	CGTAATCCTCAGTACTGGCTA	151.22028
BB93891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8081	WGS84	BB93891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93891_Sspa_Oph2007_COI	GC107	1	BB93891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93891	Sample	BB93891	targeted	Absent	Sample	Sspa_Oph2007_COI	GC107	COI	45	BB93891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC107	GAAGGCCTTCCGATGATC	151.22028
BB93891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8081	WGS84	BB93891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93891_Upin_Bot2011_COI	GC107	1	BB93891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93891	Sample	BB93891	targeted	Absent	Sample	Upin_Bot2011_COI	GC107	COI	45	BB93891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC107	CTTTAATTACAGCGTTTTTATTGTTGT	151.22028
BB93892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80503	WGS84	BB93892	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93892_Mgig_Bot2012_COI	GC103	1	BB93892_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93892	Sample	BB93892	targeted	Absent	Sample	Mgig_Bot2012_COI	GC103	COI	45	BB93892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC103	TCTTATTCGTTGGAGACTTTATAACCCT	151.21647
BB93893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80814	WGS84	BB93893	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93893_Cmae_Bot2011_COI	GC101	1	BB93893_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93893	Sample	BB93893	targeted	Absent	Sample	Cmae_Bot2011_COI	GC101	COI	45	BB93893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC101	ATGAACAGTCTATCCTCCTTTAG	151.21454
BB93893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80814	WGS84	BB93893	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93893_Msal_Bot2012_COI	GC101	1	BB93893_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93893	Sample	BB93893	targeted	Absent	Sample	Msal_Bot2012_COI	GC101	COI	45	BB93893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC101	GYTAGTTCCRATGATGTTAGCTG	151.21454
BB93895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85269	WGS84	BB93895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93895_Aamu_Bax2006_COI	GC8	1	BB93895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93895	Sample	BB93895	targeted	Absent	Sample	Aamu_Bax2006_COI	GC8	COI	45	BB93895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC8	GCACAACCGGGATCTTTACTTC	151.31994
BB93895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85269	WGS84	BB93895	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93895_Pcan_Bot2011_IGS	GC8	1	BB93895_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93895	Sample	BB93895	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC8	IGS	45	BB93895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC8	CGTAATCCTCAGTACTGGCTA	151.31994
BB93895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85269	WGS84	BB93895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93895_Sspa_Oph2007_COI	GC8	1	BB93895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93895	Sample	BB93895	targeted	Absent	Sample	Sspa_Oph2007_COI	GC8	COI	45	BB93895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC8	GAAGGCCTTCCGATGATC	151.31994
BB93895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85269	WGS84	BB93895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93895_Upin_Bot2011_COI	GC8	1	BB93895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93895	Sample	BB93895	targeted	Absent	Sample	Upin_Bot2011_COI	GC8	COI	45	BB93895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC8	CTTTAATTACAGCGTTTTTATTGTTGT	151.31994
BB93896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85242	WGS84	BB93896	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93896_Mgig_Bot2012_COI	GC6	1	BB93896_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93896	Sample	BB93896	targeted	Absent	Sample	Mgig_Bot2012_COI	GC6	COI	45	BB93896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC6	TCTTATTCGTTGGAGACTTTATAACCCT	151.31635
BB93897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85164	WGS84	BB93897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93897_Cmae_Bot2011_COI	GC3	1	BB93897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93897	Sample	BB93897	targeted	Absent	Sample	Cmae_Bot2011_COI	GC3	COI	45	BB93897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC3	ATGAACAGTCTATCCTCCTTTAG	151.3116
BB93897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85164	WGS84	BB93897	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93897_Msal_Bot2012_COI	GC3	1	BB93897_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93897	Sample	BB93897	targeted	Absent	Sample	Msal_Bot2012_COI	GC3	COI	45	BB93897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC3	GYTAGTTCCRATGATGTTAGCTG	151.3116
BB93899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79153	WGS84	BB93899	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93899_Aamu_Bax2006_COI	GC98	1	BB93899_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93899	Sample	BB93899	targeted	Absent	Sample	Aamu_Bax2006_COI	GC98	COI	45	BB93899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC98	GCACAACCGGGATCTTTACTTC	151.18068
BB93899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79153	WGS84	BB93899	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93899_Pcan_Bot2011_IGS	GC98	1	BB93899_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93899	Sample	BB93899	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC98	IGS	45	BB93899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC98	CGTAATCCTCAGTACTGGCTA	151.18068
BB93899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79153	WGS84	BB93899	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93899_Sspa_Oph2007_COI	GC98	1	BB93899_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93899	Sample	BB93899	targeted	Absent	Sample	Sspa_Oph2007_COI	GC98	COI	45	BB93899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC98	GAAGGCCTTCCGATGATC	151.18068
BB93899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79153	WGS84	BB93899	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93899_Upin_Bot2011_COI	GC98	1	BB93899_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93899	Sample	BB93899	targeted	Absent	Sample	Upin_Bot2011_COI	GC98	COI	45	BB93899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC98	CTTTAATTACAGCGTTTTTATTGTTGT	151.18068
BB93900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79182	WGS84	BB93900	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93900_Mgig_Bot2012_COI	GC96	1	BB93900_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93900	Sample	BB93900	targeted	Absent	Sample	Mgig_Bot2012_COI	GC96	COI	45	BB93900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC96	TCTTATTCGTTGGAGACTTTATAACCCT	151.18352
BB93901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79014	WGS84	BB93901	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93901_Cmae_Bot2011_COI	GC92	1	BB93901_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93901	Sample	BB93901	targeted	Absent	Sample	Cmae_Bot2011_COI	GC92	COI	45	BB93901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC92	ATGAACAGTCTATCCTCCTTTAG	151.17721
BB93901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79014	WGS84	BB93901	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93901_Msal_Bot2012_COI	GC92	1	BB93901_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93901	Sample	BB93901	targeted	Absent	Sample	Msal_Bot2012_COI	GC92	COI	45	BB93901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC92	GYTAGTTCCRATGATGTTAGCTG	151.17721
BB93903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78545	WGS84	BB93903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93903_Aamu_Bax2006_COI	GC87	1	BB93903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93903	Sample	BB93903	targeted	Absent	Sample	Aamu_Bax2006_COI	GC87	COI	45	BB93903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC87	GCACAACCGGGATCTTTACTTC	151.17523
BB93903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78545	WGS84	BB93903	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93903_Pcan_Bot2011_IGS	GC87	1	BB93903_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93903	Sample	BB93903	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC87	IGS	45	BB93903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC87	CGTAATCCTCAGTACTGGCTA	151.17523
BB93903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78545	WGS84	BB93903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93903_Sspa_Oph2007_COI	GC87	1	BB93903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93903	Sample	BB93903	targeted	Absent	Sample	Sspa_Oph2007_COI	GC87	COI	45	BB93903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC87	GAAGGCCTTCCGATGATC	151.17523
BB93903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78545	WGS84	BB93903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93903_Upin_Bot2011_COI	GC87	1	BB93903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93903	Sample	BB93903	targeted	Absent	Sample	Upin_Bot2011_COI	GC87	COI	45	BB93903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC87	CTTTAATTACAGCGTTTTTATTGTTGT	151.17523
BB93904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78472	WGS84	BB93904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93904_Mgig_Bot2012_COI	GC84	1	BB93904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93904	Sample	BB93904	targeted	Absent	Sample	Mgig_Bot2012_COI	GC84	COI	45	BB93904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC84	TCTTATTCGTTGGAGACTTTATAACCCT	151.17289
BB93905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7855	WGS84	BB93905	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93905_Cmae_Bot2011_COI	GC82	1	BB93905_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93905	Sample	BB93905	targeted	Absent	Sample	Cmae_Bot2011_COI	GC82	COI	45	BB93905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC82	ATGAACAGTCTATCCTCCTTTAG	151.17113
BB93905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7855	WGS84	BB93905	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93905_Msal_Bot2012_COI	GC82	1	BB93905_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93905	Sample	BB93905	targeted	Absent	Sample	Msal_Bot2012_COI	GC82	COI	45	BB93905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC82	GYTAGTTCCRATGATGTTAGCTG	151.17113
BC05867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83717	WGS84	BC05867	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05867_Mgig_Bot2012_COI	GC14	1	BC05867_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05867	Sample	BC05867	targeted	Absent	Sample	Mgig_Bot2012_COI	GC14	COI	45	BC05867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC14	TCTTATTCGTTGGAGACTTTATAACCCT	151.27458
BC05868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8351	WGS84	BC05868	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05868_Cmae_Bot2011_COI	GC16	1	BC05868_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05868	Sample	BC05868	targeted	Absent	Sample	Cmae_Bot2011_COI	GC16	COI	45	BC05868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC16	ATGAACAGTCTATCCTCCTTTAG	151.27295
BC05868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8351	WGS84	BC05868	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05868_Msal_Bot2012_COI	GC16	1	BC05868_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05868	Sample	BC05868	targeted	Absent	Sample	Msal_Bot2012_COI	GC16	COI	45	BC05868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC16	GYTAGTTCCRATGATGTTAGCTG	151.27295
BC05870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83558	WGS84	BC05870	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05870_Mgig_Bot2012_COI	GC19	1	BC05870_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05870	Sample	BC05870	targeted	Absent	Sample	Mgig_Bot2012_COI	GC19	COI	45	BC05870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC19	TCTTATTCGTTGGAGACTTTATAACCCT	151.26913
BC05871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83199	WGS84	BC05871	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05871_Cmae_Bot2011_COI	GC21	1	BC05871_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05871	Sample	BC05871	targeted	Absent	Sample	Cmae_Bot2011_COI	GC21	COI	45	BC05871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC21	ATGAACAGTCTATCCTCCTTTAG	151.25933
BC05871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83199	WGS84	BC05871	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05871_Msal_Bot2012_COI	GC21	1	BC05871_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05871	Sample	BC05871	targeted	Absent	Sample	Msal_Bot2012_COI	GC21	COI	45	BC05871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC21	GYTAGTTCCRATGATGTTAGCTG	151.25933
BC05873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83485	WGS84	BC05873	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05873_Aamu_Bax2006_COI	GC34	1	BC05873_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05873	Sample	BC05873	targeted	Absent	Sample	Aamu_Bax2006_COI	GC34	COI	45	BC05873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC34	GCACAACCGGGATCTTTACTTC	151.26891
BC05873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83485	WGS84	BC05873	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05873_Pcan_Bot2011_IGS	GC34	1	BC05873_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05873	Sample	BC05873	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC34	IGS	45	BC05873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC34	CGTAATCCTCAGTACTGGCTA	151.26891
BC05873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83485	WGS84	BC05873	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05873_Sspa_Oph2007_COI	GC34	1	BC05873_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05873	Sample	BC05873	targeted	Absent	Sample	Sspa_Oph2007_COI	GC34	COI	45	BC05873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC34	GAAGGCCTTCCGATGATC	151.26891
BC05873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83485	WGS84	BC05873	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05873_Upin_Bot2011_COI	GC34	1	BC05873_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05873	Sample	BC05873	targeted	Absent	Sample	Upin_Bot2011_COI	GC34	COI	45	BC05873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC34	CTTTAATTACAGCGTTTTTATTGTTGT	151.26891
BC05874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83216	WGS84	BC05874	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05874_Mgig_Bot2012_COI	GC37	1	BC05874_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05874	Sample	BC05874	targeted	Absent	Sample	Mgig_Bot2012_COI	GC37	COI	45	BC05874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC37	TCTTATTCGTTGGAGACTTTATAACCCT	151.26233
BC05875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79288	WGS84	BC05875	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05875_Cmae_Bot2011_COI	GC71	1	BC05875_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05875	Sample	BC05875	targeted	Absent	Sample	Cmae_Bot2011_COI	GC71	COI	45	BC05875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC71	ATGAACAGTCTATCCTCCTTTAG	151.2117
BC05875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79288	WGS84	BC05875	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05875_Msal_Bot2012_COI	GC71	1	BC05875_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05875	Sample	BC05875	targeted	Absent	Sample	Msal_Bot2012_COI	GC71	COI	45	BC05875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC71	GYTAGTTCCRATGATGTTAGCTG	151.2117
BC05877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79074	WGS84	BC05877	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05877_Aamu_Bax2006_COI	GC73	1	BC05877_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05877	Sample	BC05877	targeted	Absent	Sample	Aamu_Bax2006_COI	GC73	COI	45	BC05877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC73	GCACAACCGGGATCTTTACTTC	151.20948
BC05877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79074	WGS84	BC05877	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05877_Pcan_Bot2011_IGS	GC73	1	BC05877_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05877	Sample	BC05877	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC73	IGS	45	BC05877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC73	CGTAATCCTCAGTACTGGCTA	151.20948
BC05877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79074	WGS84	BC05877	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05877_Sspa_Oph2007_COI	GC73	1	BC05877_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05877	Sample	BC05877	targeted	Absent	Sample	Sspa_Oph2007_COI	GC73	COI	45	BC05877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC73	GAAGGCCTTCCGATGATC	151.20948
BC05877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79074	WGS84	BC05877	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05877_Upin_Bot2011_COI	GC73	1	BC05877_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05877	Sample	BC05877	targeted	Absent	Sample	Upin_Bot2011_COI	GC73	COI	45	BC05877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC73	CTTTAATTACAGCGTTTTTATTGTTGT	151.20948
BC05878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81709	WGS84	BC05878	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05878_Mgig_Bot2012_COI	GC24	1	BC05878_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05878	Sample	BC05878	targeted	Absent	Sample	Mgig_Bot2012_COI	GC24	COI	45	BC05878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC24	TCTTATTCGTTGGAGACTTTATAACCCT	151.23804
BC05879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81491	WGS84	BC05879	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05879_Cmae_Bot2011_COI	GC26	1	BC05879_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05879	Sample	BC05879	targeted	Absent	Sample	Cmae_Bot2011_COI	GC26	COI	45	BC05879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC26	ATGAACAGTCTATCCTCCTTTAG	151.23674
BC05879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81491	WGS84	BC05879	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05879_Msal_Bot2012_COI	GC26	1	BC05879_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05879	Sample	BC05879	targeted	Absent	Sample	Msal_Bot2012_COI	GC26	COI	45	BC05879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC26	GYTAGTTCCRATGATGTTAGCTG	151.23674
BC05880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81635	WGS84	BC05880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05880_Aamu_Bax2006_COI	GC27	1	BC05880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05880	Sample	BC05880	targeted	Absent	Sample	Aamu_Bax2006_COI	GC27	COI	45	BC05880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC27	GCACAACCGGGATCTTTACTTC	151.23642
BC05880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81635	WGS84	BC05880	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05880_Pcan_Bot2011_IGS	GC27	1	BC05880_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05880	Sample	BC05880	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC27	IGS	45	BC05880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC27	CGTAATCCTCAGTACTGGCTA	151.23642
BC05880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81635	WGS84	BC05880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05880_Sspa_Oph2007_COI	GC27	1	BC05880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05880	Sample	BC05880	targeted	Absent	Sample	Sspa_Oph2007_COI	GC27	COI	45	BC05880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC27	GAAGGCCTTCCGATGATC	151.23642
BC05880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81635	WGS84	BC05880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05880_Upin_Bot2011_COI	GC27	1	BC05880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05880	Sample	BC05880	targeted	Absent	Sample	Upin_Bot2011_COI	GC27	COI	45	BC05880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC27	CTTTAATTACAGCGTTTTTATTGTTGT	151.23642
BC05881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81904	WGS84	BC05881	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05881_Mgig_Bot2012_COI	GC29	1	BC05881_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05881	Sample	BC05881	targeted	Absent	Sample	Mgig_Bot2012_COI	GC29	COI	45	BC05881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC29	TCTTATTCGTTGGAGACTTTATAACCCT	151.24007
BC05882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82101	WGS84	BC05882	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05882_Cmae_Bot2011_COI	GC31	1	BC05882_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05882	Sample	BC05882	targeted	Absent	Sample	Cmae_Bot2011_COI	GC31	COI	45	BC05882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC31	ATGAACAGTCTATCCTCCTTTAG	151.24208
BC05882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82101	WGS84	BC05882	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05882_Msal_Bot2012_COI	GC31	1	BC05882_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05882	Sample	BC05882	targeted	Absent	Sample	Msal_Bot2012_COI	GC31	COI	45	BC05882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC31	GYTAGTTCCRATGATGTTAGCTG	151.24208
BC05884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82267	WGS84	BC05884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05884_Aamu_Bax2006_COI	GC42	1	BC05884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05884	Sample	BC05884	targeted	Absent	Sample	Aamu_Bax2006_COI	GC42	COI	45	BC05884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC42	GCACAACCGGGATCTTTACTTC	151.24423
BC05884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82267	WGS84	BC05884	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05884_Pcan_Bot2011_IGS	GC42	1	BC05884_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05884	Sample	BC05884	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC42	IGS	45	BC05884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC42	CGTAATCCTCAGTACTGGCTA	151.24423
BC05884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82267	WGS84	BC05884	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05884_Sspa_Oph2007_COI	GC42	1	BC05884_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05884	Sample	BC05884	targeted	Absent	Sample	Sspa_Oph2007_COI	GC42	COI	45	BC05884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC42	GAAGGCCTTCCGATGATC	151.24423
BC05884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82267	WGS84	BC05884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05884_Upin_Bot2011_COI	GC42	1	BC05884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05884	Sample	BC05884	targeted	Absent	Sample	Upin_Bot2011_COI	GC42	COI	45	BC05884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC42	CTTTAATTACAGCGTTTTTATTGTTGT	151.24423
BC05885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8232	WGS84	BC05885	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05885_Mgig_Bot2012_COI	GC44	1	BC05885_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05885	Sample	BC05885	targeted	Absent	Sample	Mgig_Bot2012_COI	GC44	COI	45	BC05885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC44	TCTTATTCGTTGGAGACTTTATAACCCT	151.24118
BC05886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8224	WGS84	BC05886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05886_Cmae_Bot2011_COI	GC49	1	BC05886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05886	Sample	BC05886	targeted	Absent	Sample	Cmae_Bot2011_COI	GC49	COI	45	BC05886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC49	ATGAACAGTCTATCCTCCTTTAG	151.24152
BC05886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8224	WGS84	BC05886	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05886_Msal_Bot2012_COI	GC49	1	BC05886_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05886	Sample	BC05886	targeted	Absent	Sample	Msal_Bot2012_COI	GC49	COI	45	BC05886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC49	GYTAGTTCCRATGATGTTAGCTG	151.24152
BC05888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81964	WGS84	BC05888	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05888_Aamu_Bax2006_COI	GC53	1	BC05888_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05888	Sample	BC05888	targeted	Absent	Sample	Aamu_Bax2006_COI	GC53	COI	45	BC05888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC53	GCACAACCGGGATCTTTACTTC	151.23886
BC05888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81964	WGS84	BC05888	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05888_Pcan_Bot2011_IGS	GC53	1	BC05888_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05888	Sample	BC05888	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC53	IGS	45	BC05888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC53	CGTAATCCTCAGTACTGGCTA	151.23886
BC05888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81964	WGS84	BC05888	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05888_Sspa_Oph2007_COI	GC53	1	BC05888_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05888	Sample	BC05888	targeted	Absent	Sample	Sspa_Oph2007_COI	GC53	COI	45	BC05888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC53	GAAGGCCTTCCGATGATC	151.23886
BC05888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81964	WGS84	BC05888	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05888_Upin_Bot2011_COI	GC53	1	BC05888_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05888	Sample	BC05888	targeted	Absent	Sample	Upin_Bot2011_COI	GC53	COI	45	BC05888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC53	CTTTAATTACAGCGTTTTTATTGTTGT	151.23886
BC05889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82546	WGS84	BC05889	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05889_Mgig_Bot2012_COI	GC56	1	BC05889_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05889	Sample	BC05889	targeted	Absent	Sample	Mgig_Bot2012_COI	GC56	COI	45	BC05889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC56	TCTTATTCGTTGGAGACTTTATAACCCT	151.24655
BC05891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82553	WGS84	BC05891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05891_Aamu_Bax2006_COI	GC61	1	BC05891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05891	Sample	BC05891	targeted	Absent	Sample	Aamu_Bax2006_COI	GC61	COI	45	BC05891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC61	GCACAACCGGGATCTTTACTTC	151.2492
BC05891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82553	WGS84	BC05891	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05891_Pcan_Bot2011_IGS	GC61	1	BC05891_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05891	Sample	BC05891	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC61	IGS	45	BC05891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC61	CGTAATCCTCAGTACTGGCTA	151.2492
BC05891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82553	WGS84	BC05891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05891_Sspa_Oph2007_COI	GC61	1	BC05891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05891	Sample	BC05891	targeted	Absent	Sample	Sspa_Oph2007_COI	GC61	COI	45	BC05891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC61	GAAGGCCTTCCGATGATC	151.2492
BC05891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82553	WGS84	BC05891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05891_Upin_Bot2011_COI	GC61	1	BC05891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05891	Sample	BC05891	targeted	Absent	Sample	Upin_Bot2011_COI	GC61	COI	45	BC05891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC61	CTTTAATTACAGCGTTTTTATTGTTGT	151.2492
BC05892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82287	WGS84	BC05892	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05892_Mgig_Bot2012_COI	GC62	1	BC05892_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05892	Sample	BC05892	targeted	Absent	Sample	Mgig_Bot2012_COI	GC62	COI	45	BC05892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC62	TCTTATTCGTTGGAGACTTTATAACCCT	151.24617
BC05893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81925	WGS84	BC05893	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05893_Cmae_Bot2011_COI	GC63	1	BC05893_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05893	Sample	BC05893	targeted	Absent	Sample	Cmae_Bot2011_COI	GC63	COI	45	BC05893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC63	ATGAACAGTCTATCCTCCTTTAG	151.24293
BC05893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81925	WGS84	BC05893	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05893_Msal_Bot2012_COI	GC63	1	BC05893_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05893	Sample	BC05893	targeted	Absent	Sample	Msal_Bot2012_COI	GC63	COI	45	BC05893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC63	GYTAGTTCCRATGATGTTAGCTG	151.24293
BC05895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81432	WGS84	BC05895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05895_Aamu_Bax2006_COI	GC66	1	BC05895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05895	Sample	BC05895	targeted	Absent	Sample	Aamu_Bax2006_COI	GC66	COI	45	BC05895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC66	GCACAACCGGGATCTTTACTTC	151.23819
BC05895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81432	WGS84	BC05895	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05895_Pcan_Bot2011_IGS	GC66	1	BC05895_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05895	Sample	BC05895	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC66	IGS	45	BC05895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC66	CGTAATCCTCAGTACTGGCTA	151.23819
BC05895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81432	WGS84	BC05895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05895_Sspa_Oph2007_COI	GC66	1	BC05895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05895	Sample	BC05895	targeted	Absent	Sample	Sspa_Oph2007_COI	GC66	COI	45	BC05895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC66	GAAGGCCTTCCGATGATC	151.23819
BC05895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81432	WGS84	BC05895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05895_Upin_Bot2011_COI	GC66	1	BC05895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05895	Sample	BC05895	targeted	Absent	Sample	Upin_Bot2011_COI	GC66	COI	45	BC05895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC66	CTTTAATTACAGCGTTTTTATTGTTGT	151.23819
BC05896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82193	WGS84	BC05896	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05896_Mgig_Bot2012_COI	GC111	1	BC05896_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05896	Sample	BC05896	targeted	Absent	Sample	Mgig_Bot2012_COI	GC111	COI	45	BC05896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC111	TCTTATTCGTTGGAGACTTTATAACCCT	151.24544
BC05897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78028	WGS84	BC05897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05897_Cmae_Bot2011_COI	GC77	1	BC05897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05897	Sample	BC05897	targeted	Absent	Sample	Cmae_Bot2011_COI	GC77	COI	45	BC05897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC77	ATGAACAGTCTATCCTCCTTTAG	151.19927
BC05897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78028	WGS84	BC05897	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05897_Msal_Bot2012_COI	GC77	1	BC05897_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05897	Sample	BC05897	targeted	Absent	Sample	Msal_Bot2012_COI	GC77	COI	45	BC05897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC77	GYTAGTTCCRATGATGTTAGCTG	151.19927
BC05899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.77805	WGS84	BC05899	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05899_Aamu_Bax2006_COI	GC81	1	BC05899_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05899	Sample	BC05899	targeted	Absent	Sample	Aamu_Bax2006_COI	GC81	COI	45	BC05899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC81	GCACAACCGGGATCTTTACTTC	151.19493
BC05899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.77805	WGS84	BC05899	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05899_Pcan_Bot2011_IGS	GC81	1	BC05899_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05899	Sample	BC05899	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC81	IGS	45	BC05899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC81	CGTAATCCTCAGTACTGGCTA	151.19493
BC05899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.77805	WGS84	BC05899	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05899_Sspa_Oph2007_COI	GC81	1	BC05899_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05899	Sample	BC05899	targeted	Absent	Sample	Sspa_Oph2007_COI	GC81	COI	45	BC05899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC81	GAAGGCCTTCCGATGATC	151.19493
BC05899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.77805	WGS84	BC05899	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05899_Upin_Bot2011_COI	GC81	1	BC05899_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05899	Sample	BC05899	targeted	Absent	Sample	Upin_Bot2011_COI	GC81	COI	45	BC05899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC81	CTTTAATTACAGCGTTTTTATTGTTGT	151.19493
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06765_Cjap_Sim2018_COI	T5-01	1	BC06765_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81438
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	17/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06765_Mgig_Bot2012_COI	T5-01	1	BC06765_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.81438
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06765_Mjap_Gib2022_COI	T5-01	1	BC06765_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	AATTTAATTGTGACTAGGCATGGTCTG	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06766_Cmae_Bot2011_COI	T5-02	1	BC06766_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	ATGAACAGTCTATCCTCCTTTAG	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06766_Mros_Gib2022_COI	T5-02	1	BC06766_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Absent	Sample	Mros_Gib2022_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81438
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06768_Aamu_Bax2006_COI	T5-04	1	BC06768_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Aamu_Bax2006_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	GCACAACCGGGATCTTTACTTC	147.87403
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06768_Pcan_Bot2011_IGS	T5-04	1	BC06768_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-04	IGS	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	CGTAATCCTCAGTACTGGCTA	147.87403
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06768_Sspa_Oph2007_COI	T5-04	1	BC06768_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	GAAGGCCTTCCGATGATC	147.87403
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06768_Upin_Bot2011_COI	T5-04	1	BC06768_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Upin_Bot2011_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.87403
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06769_Cjap_Sim2018_COI	T5-05	1	BC06769_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87326
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	17/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06769_Mgig_Bot2012_COI	T5-05	1	BC06769_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.87326
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06769_Mjap_Gib2022_COI	T5-05	1	BC06769_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	AATTTAATTGTGACTAGGCATGGTCTG	147.87326
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.031	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06771_Aamu_Bax2006_COI	T5-07	1	BC06771_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Present	Sample	Aamu_Bax2006_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	GCACAACCGGGATCTTTACTTC	147.30203
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06771_Pcan_Bot2011_IGS	T5-07	1	BC06771_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-07	IGS	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	CGTAATCCTCAGTACTGGCTA	147.30203
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06771_Sspa_Oph2007_COI	T5-07	1	BC06771_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	GAAGGCCTTCCGATGATC	147.30203
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06771_Upin_Bot2011_COI	T5-07	1	BC06771_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Upin_Bot2011_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.30203
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06772_Cjap_Sim2018_COI	T5-08	1	BC06772_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3022
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06772_Mgig_Bot2012_COI	T5-08	1	BC06772_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.3022
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06772_Mjap_Gib2022_COI	T5-08	1	BC06772_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	AATTTAATTGTGACTAGGCATGGTCTG	147.3022
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06773_Cmae_Bot2011_COI	T5-09	1	BC06773_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	ATGAACAGTCTATCCTCCTTTAG	147.30444
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06773_Mros_Gib2022_COI	T5-09	1	BC06773_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Mros_Gib2022_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30444
BC06775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87812	WGS84	BC06775	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.731	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06775_Aamu_Bax2006_COI	T5-11	1	BC06775_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06775	Sample	BC06775	targeted	Present	Sample	Aamu_Bax2006_COI	T5-11	COI	45	BC06775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-11	GCACAACCGGGATCTTTACTTC	147.34163
BC06775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87812	WGS84	BC06775	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06775_Pcan_Bot2011_IGS	T5-11	1	BC06775_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06775	Sample	BC06775	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-11	IGS	45	BC06775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-11	CGTAATCCTCAGTACTGGCTA	147.34163
BC06775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87812	WGS84	BC06775	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06775_Sspa_Oph2007_COI	T5-11	1	BC06775_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06775	Sample	BC06775	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-11	COI	45	BC06775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-11	GAAGGCCTTCCGATGATC	147.34163
BC06775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87812	WGS84	BC06775	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06775_Upin_Bot2011_COI	T5-11	1	BC06775_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06775	Sample	BC06775	targeted	Absent	Sample	Upin_Bot2011_COI	T5-11	COI	45	BC06775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.34163
BC06776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88112	WGS84	BC06776	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06776_Cjap_Sim2018_COI	T5-12	1	BC06776_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06776	Sample	BC06776	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-12	COI	45	BC06776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34198
BC06776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88112	WGS84	BC06776	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06776_Mgig_Bot2012_COI	T5-12	1	BC06776_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06776	Sample	BC06776	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-12	COI	45	BC06776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.34198
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06777_Cmae_Bot2011_COI	T5-13	1	BC06777_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	ATGAACAGTCTATCCTCCTTTAG	147.33945
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.397	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06777_Mros_Gib2022_COI	T5-13	1	BC06777_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Present	Sample	Mros_Gib2022_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33945
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.702	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06779_Aamu_Bax2006_COI	T5-15	1	BC06779_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Present	Sample	Aamu_Bax2006_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	GCACAACCGGGATCTTTACTTC	147.53158
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06779_Pcan_Bot2011_IGS	T5-15	1	BC06779_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-15	IGS	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	CGTAATCCTCAGTACTGGCTA	147.53158
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06779_Sspa_Oph2007_COI	T5-15	1	BC06779_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	GAAGGCCTTCCGATGATC	147.53158
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06779_Upin_Bot2011_COI	T5-15	1	BC06779_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Absent	Sample	Upin_Bot2011_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.53158
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.916	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06780_Cmae_Bot2011_COI	T5-16	1	BC06780_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Present	Sample	Cmae_Bot2011_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	ATGAACAGTCTATCCTCCTTTAG	147.53983
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.119	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06780_Mros_Gib2022_COI	T5-16	1	BC06780_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Present	Sample	Mros_Gib2022_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53983
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.312	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06782_Aamu_Bax2006_COI	T5-18	1	BC06782_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Present	Sample	Aamu_Bax2006_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	GCACAACCGGGATCTTTACTTC	147.53312
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06782_Pcan_Bot2011_IGS	T5-18	1	BC06782_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-18	IGS	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	CGTAATCCTCAGTACTGGCTA	147.53312
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06782_Sspa_Oph2007_COI	T5-18	1	BC06782_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	GAAGGCCTTCCGATGATC	147.53312
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06782_Upin_Bot2011_COI	T5-18	1	BC06782_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Absent	Sample	Upin_Bot2011_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	CTTTAATTACAGCGTTTTTATTGTTGT	147.53312
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06783_Cjap_Sim2018_COI	T5-19	1	BC06783_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51957
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	26/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06783_Mgig_Bot2012_COI	T5-19	1	BC06783_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	TCTTATTCGTTGGAGACTTTATAACCCT	147.51957
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06783_Mjap_Gib2022_COI	T5-19	1	BC06783_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	AATTTAATTGTGACTAGGCATGGTCTG	147.51957
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.634	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06784_Cmae_Bot2011_COI	T6-01	1	BC06784_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Present	Sample	Cmae_Bot2011_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	ATGAACAGTCTATCCTCCTTTAG	147.30679
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06784_Mros_Gib2022_COI	T6-01	1	BC06784_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Absent	Sample	Mros_Gib2022_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30679
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.8	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06786_Aamu_Bax2006_COI	T6-03	1	BC06786_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Present	Sample	Aamu_Bax2006_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	GCACAACCGGGATCTTTACTTC	147.30193
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06786_Pcan_Bot2011_IGS	T6-03	1	BC06786_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-03	IGS	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	CGTAATCCTCAGTACTGGCTA	147.30193
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06786_Sspa_Oph2007_COI	T6-03	1	BC06786_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	GAAGGCCTTCCGATGATC	147.30193
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06786_Upin_Bot2011_COI	T6-03	1	BC06786_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Absent	Sample	Upin_Bot2011_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.30193
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06787_Cjap_Sim2018_COI	T6-04	1	BC06787_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33639
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.947	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06787_Mgig_Bot2012_COI	T6-04	1	BC06787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Present	Sample	Mgig_Bot2012_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.33639
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06787_Mjap_Gib2022_COI	T6-04	1	BC06787_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	AATTTAATTGTGACTAGGCATGGTCTG	147.33639
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06788_Cmae_Bot2011_COI	T6-05	1	BC06788_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	ATGAACAGTCTATCCTCCTTTAG	147.34157
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06788_Mros_Gib2022_COI	T6-05	1	BC06788_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Absent	Sample	Mros_Gib2022_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34157
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06790_Cjap_Sim2018_COI	T6-07	1	BC06790_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33483
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.159	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06790_Mgig_Bot2012_COI	T6-07	1	BC06790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Present	Sample	Mgig_Bot2012_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.33483
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06790_Mjap_Gib2022_COI	T6-07	1	BC06790_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	AATTTAATTGTGACTAGGCATGGTCTG	147.33483
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06791_Cmae_Bot2011_COI	T6-08	1	BC06791_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	ATGAACAGTCTATCCTCCTTTAG	147.33618
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06791_Mros_Gib2022_COI	T6-08	1	BC06791_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Absent	Sample	Mros_Gib2022_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33618
BC06793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9627	WGS84	BC06793	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.498	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06793_Aamu_Bax2006_COI	T6-10	1	BC06793_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06793	Sample	BC06793	targeted	Present	Sample	Aamu_Bax2006_COI	T6-10	COI	45	BC06793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-10	GCACAACCGGGATCTTTACTTC	147.53935
BC06793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9627	WGS84	BC06793	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06793_Pcan_Bot2011_IGS	T6-10	1	BC06793_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06793	Sample	BC06793	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-10	IGS	45	BC06793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-10	CGTAATCCTCAGTACTGGCTA	147.53935
BC06793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9627	WGS84	BC06793	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06793_Sspa_Oph2007_COI	T6-10	1	BC06793_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06793	Sample	BC06793	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-10	COI	45	BC06793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-10	GAAGGCCTTCCGATGATC	147.53935
BC06793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9627	WGS84	BC06793	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06793_Upin_Bot2011_COI	T6-10	1	BC06793_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06793	Sample	BC06793	targeted	Absent	Sample	Upin_Bot2011_COI	T6-10	COI	45	BC06793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.53935
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06794_Cjap_Sim2018_COI	T6-11	1	BC06794_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53139
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	26/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06794_Mgig_Bot2012_COI	T6-11	1	BC06794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Absent	Sample	Mgig_Bot2012_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.53139
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06794_Mjap_Gib2022_COI	T6-11	1	BC06794_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	AATTTAATTGTGACTAGGCATGGTCTG	147.53139
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.339	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06795_Cmae_Bot2011_COI	T6-12	1	BC06795_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Present	Sample	Cmae_Bot2011_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	ATGAACAGTCTATCCTCCTTTAG	147.51949
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.738	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06795_Mros_Gib2022_COI	T6-12	1	BC06795_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Present	Sample	Mros_Gib2022_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.51949
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06797_Aamu_Bax2006_COI	T6-14	1	BC06797_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Absent	Sample	Aamu_Bax2006_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	GCACAACCGGGATCTTTACTTC	147.8762
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06797_Pcan_Bot2011_IGS	T6-14	1	BC06797_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-14	IGS	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	CGTAATCCTCAGTACTGGCTA	147.8762
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06797_Sspa_Oph2007_COI	T6-14	1	BC06797_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	GAAGGCCTTCCGATGATC	147.8762
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.463	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06797_Upin_Bot2011_COI	T6-14	1	BC06797_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Present	Sample	Upin_Bot2011_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.8762
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06798_Cjap_Sim2018_COI	T6-15	1	BC06798_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-15	COI	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87386
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	42.758	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	28/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06798_Mgig_Bot2012_COI	T6-15	1	BC06798_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Present	Sample	Mgig_Bot2012_COI	T6-15	COI	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.87386
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.713	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06799_Cmae_Bot2011_COI	T6-16	1	BC06799_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Present	Sample	Cmae_Bot2011_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	ATGAACAGTCTATCCTCCTTTAG	147.87236
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.534	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06799_Mros_Gib2022_COI	T6-16	1	BC06799_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Present	Sample	Mros_Gib2022_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87236
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06801_Aamu_Bax2006_COI	T7-02	1	BC06801_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Absent	Sample	Aamu_Bax2006_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	GCACAACCGGGATCTTTACTTC	147.87573
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06801_Pcan_Bot2011_IGS	T7-02	1	BC06801_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-02	IGS	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	CGTAATCCTCAGTACTGGCTA	147.87573
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06801_Sspa_Oph2007_COI	T7-02	1	BC06801_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	GAAGGCCTTCCGATGATC	147.87573
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.797	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06801_Upin_Bot2011_COI	T7-02	1	BC06801_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Present	Sample	Upin_Bot2011_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.87573
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06802_Cjap_Sim2018_COI	T7-03	1	BC06802_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87392
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.009	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06802_Mgig_Bot2012_COI	T7-03	1	BC06802_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Present	Sample	Mgig_Bot2012_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.87392
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06802_Mjap_Gib2022_COI	T7-03	1	BC06802_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	AATTTAATTGTGACTAGGCATGGTCTG	147.87392
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06803_Cmae_Bot2011_COI	T7-04	1	BC06803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Absent	Sample	Cmae_Bot2011_COI	T7-04	COI	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	ATGAACAGTCTATCCTCCTTTAG	147.87248
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	30.432	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06803_Mros_Gib2022_COI	T7-04	1	BC06803_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Present	Sample	Mros_Gib2022_COI	T7-04	COI	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87248
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.147	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06805_Aamu_Bax2006_COI	T7-06	1	BC06805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Present	Sample	Aamu_Bax2006_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	GCACAACCGGGATCTTTACTTC	147.30286
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06805_Pcan_Bot2011_IGS	T7-06	1	BC06805_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-06	IGS	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	CGTAATCCTCAGTACTGGCTA	147.30286
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06805_Sspa_Oph2007_COI	T7-06	1	BC06805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	GAAGGCCTTCCGATGATC	147.30286
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06805_Upin_Bot2011_COI	T7-06	1	BC06805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Absent	Sample	Upin_Bot2011_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.30286
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06806_Cjap_Sim2018_COI	T7-07	1	BC06806_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30118
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.445	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06806_Mgig_Bot2012_COI	T7-07	1	BC06806_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Present	Sample	Mgig_Bot2012_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.30118
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06806_Mjap_Gib2022_COI	T7-07	1	BC06806_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	AATTTAATTGTGACTAGGCATGGTCTG	147.30118
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.643	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06807_Cmae_Bot2011_COI	T7-08	1	BC06807_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Present	Sample	Cmae_Bot2011_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	ATGAACAGTCTATCCTCCTTTAG	147.33611
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.614	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06807_Mros_Gib2022_COI	T7-08	1	BC06807_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Present	Sample	Mros_Gib2022_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33611
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.656	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06809_Aamu_Bax2006_COI	T7-10	1	BC06809_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Present	Sample	Aamu_Bax2006_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	GCACAACCGGGATCTTTACTTC	147.34175
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06809_Pcan_Bot2011_IGS	T7-10	1	BC06809_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-10	IGS	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	CGTAATCCTCAGTACTGGCTA	147.34175
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06809_Sspa_Oph2007_COI	T7-10	1	BC06809_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	GAAGGCCTTCCGATGATC	147.34175
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06809_Upin_Bot2011_COI	T7-10	1	BC06809_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Absent	Sample	Upin_Bot2011_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.34175
BC06810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88559	WGS84	BC06810	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06810_Cmae_Bot2011_COI	T7-11	1	BC06810_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06810	Sample	BC06810	targeted	Absent	Sample	Cmae_Bot2011_COI	T7-11	COI	45	BC06810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-11	ATGAACAGTCTATCCTCCTTTAG	147.33519
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.37	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06812_Aamu_Bax2006_COI	T7-13	1	BC06812_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Present	Sample	Aamu_Bax2006_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	GCACAACCGGGATCTTTACTTC	147.33619
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06812_Pcan_Bot2011_IGS	T7-13	1	BC06812_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-13	IGS	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	CGTAATCCTCAGTACTGGCTA	147.33619
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06812_Sspa_Oph2007_COI	T7-13	1	BC06812_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	GAAGGCCTTCCGATGATC	147.33619
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06812_Upin_Bot2011_COI	T7-13	1	BC06812_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Absent	Sample	Upin_Bot2011_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.33619
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06813_Cjap_Sim2018_COI	T7-14	1	BC06813_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53937
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.454	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	28/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06813_Mgig_Bot2012_COI	T7-14	1	BC06813_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Present	Sample	Mgig_Bot2012_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.53937
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06813_Mjap_Gib2022_COI	T7-14	1	BC06813_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	AATTTAATTGTGACTAGGCATGGTCTG	147.53937
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.198	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06814_Cmae_Bot2011_COI	T7-15	1	BC06814_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Present	Sample	Cmae_Bot2011_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	ATGAACAGTCTATCCTCCTTTAG	147.53178
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.639	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06814_Mros_Gib2022_COI	T7-15	1	BC06814_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Present	Sample	Mros_Gib2022_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53178
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06816_Aamu_Bax2006_COI	T8-01	1	BC06816_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Absent	Sample	Aamu_Bax2006_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	GCACAACCGGGATCTTTACTTC	147.81263
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06816_Pcan_Bot2011_IGS	T8-01	1	BC06816_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-01	IGS	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	CGTAATCCTCAGTACTGGCTA	147.81263
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06816_Sspa_Oph2007_COI	T8-01	1	BC06816_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	GAAGGCCTTCCGATGATC	147.81263
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.154	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06816_Upin_Bot2011_COI	T8-01	1	BC06816_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Present	Sample	Upin_Bot2011_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.81263
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06817_Cjap_Sim2018_COI	T8-02	1	BC06817_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87661
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.691	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	17/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06817_Mgig_Bot2012_COI	T8-02	1	BC06817_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Present	Sample	Mgig_Bot2012_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.87661
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06817_Mjap_Gib2022_COI	T8-02	1	BC06817_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	AATTTAATTGTGACTAGGCATGGTCTG	147.87661
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06818_Cmae_Bot2011_COI	T8-03	1	BC06818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	ATGAACAGTCTATCCTCCTTTAG	147.87373
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.572	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06818_Mros_Gib2022_COI	T8-03	1	BC06818_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Present	Sample	Mros_Gib2022_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87373
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06820_Cjap_Sim2018_COI	T8-05	1	BC06820_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81282
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.495	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	17/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06820_Mgig_Bot2012_COI	T8-05	1	BC06820_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Present	Sample	Mgig_Bot2012_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.81282
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06820_Mjap_Gib2022_COI	T8-05	1	BC06820_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	AATTTAATTGTGACTAGGCATGGTCTG	147.81282
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06821_Cmae_Bot2011_COI	T8-06	1	BC06821_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	ATGAACAGTCTATCCTCCTTTAG	147.3021
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06821_Mros_Gib2022_COI	T8-06	1	BC06821_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Absent	Sample	Mros_Gib2022_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.3021
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.514	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06823_Aamu_Bax2006_COI	T8-08	1	BC06823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Present	Sample	Aamu_Bax2006_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	GCACAACCGGGATCTTTACTTC	147.30793
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06823_Pcan_Bot2011_IGS	T8-08	1	BC06823_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-08	IGS	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	CGTAATCCTCAGTACTGGCTA	147.30793
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06823_Sspa_Oph2007_COI	T8-08	1	BC06823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	GAAGGCCTTCCGATGATC	147.30793
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06823_Upin_Bot2011_COI	T8-08	1	BC06823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Absent	Sample	Upin_Bot2011_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.30793
BC06824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87309	WGS84	BC06824	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06824_Cjap_Sim2018_COI	T8-09	1	BC06824_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06824	Sample	BC06824	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-09	COI	45	BC06824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33661
BC06824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87309	WGS84	BC06824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	23.163	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06824_Mgig_Bot2012_COI	T8-09	1	BC06824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06824	Sample	BC06824	targeted	Present	Sample	Mgig_Bot2012_COI	T8-09	COI	45	BC06824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.33661
BC06825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88173	WGS84	BC06825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.142	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06825_Cmae_Bot2011_COI	T8-10	1	BC06825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06825	Sample	BC06825	targeted	Present	Sample	Cmae_Bot2011_COI	T8-10	COI	45	BC06825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-10	ATGAACAGTCTATCCTCCTTTAG	147.34203
BC06827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88574	WGS84	BC06827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.571	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06827_Aamu_Bax2006_COI	T8-12	1	BC06827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06827	Sample	BC06827	targeted	Present	Sample	Aamu_Bax2006_COI	T8-12	COI	45	BC06827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-12	GCACAACCGGGATCTTTACTTC	147.3353
BC06827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88574	WGS84	BC06827	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06827_Pcan_Bot2011_IGS	T8-12	1	BC06827_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06827	Sample	BC06827	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-12	IGS	45	BC06827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-12	CGTAATCCTCAGTACTGGCTA	147.3353
BC06827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88574	WGS84	BC06827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06827_Sspa_Oph2007_COI	T8-12	1	BC06827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06827	Sample	BC06827	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-12	COI	45	BC06827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-12	GAAGGCCTTCCGATGATC	147.3353
BC06827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88574	WGS84	BC06827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06827_Upin_Bot2011_COI	T8-12	1	BC06827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06827	Sample	BC06827	targeted	Absent	Sample	Upin_Bot2011_COI	T8-12	COI	45	BC06827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.3353
BC06828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88425	WGS84	BC06828	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06828_Cjap_Sim2018_COI	T8-13	1	BC06828_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06828	Sample	BC06828	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-13	COI	45	BC06828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33592
BC06828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88425	WGS84	BC06828	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.017	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06828_Mgig_Bot2012_COI	T8-13	1	BC06828_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06828	Sample	BC06828	targeted	Present	Sample	Mgig_Bot2012_COI	T8-13	COI	45	BC06828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.33592
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.015	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06829_Cmae_Bot2011_COI	T8-14	1	BC06829_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Present	Sample	Cmae_Bot2011_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	ATGAACAGTCTATCCTCCTTTAG	147.33624
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	33.103	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06829_Mros_Gib2022_COI	T8-14	1	BC06829_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Present	Sample	Mros_Gib2022_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33624
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.657	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06830_Aamu_Bax2006_COI	T8-15	1	BC06830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Present	Sample	Aamu_Bax2006_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	GCACAACCGGGATCTTTACTTC	147.53978
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06830_Pcan_Bot2011_IGS	T8-15	1	BC06830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-15	IGS	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	CGTAATCCTCAGTACTGGCTA	147.53978
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06830_Sspa_Oph2007_COI	T8-15	1	BC06830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	GAAGGCCTTCCGATGATC	147.53978
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06830_Upin_Bot2011_COI	T8-15	1	BC06830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Absent	Sample	Upin_Bot2011_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.53978
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06831_Cjap_Sim2018_COI	T8-16	1	BC06831_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51943
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	21.348	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	28/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06831_Mgig_Bot2012_COI	T8-16	1	BC06831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Present	Sample	Mgig_Bot2012_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.51943
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06831_Mjap_Gib2022_COI	T8-16	1	BC06831_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	AATTTAATTGTGACTAGGCATGGTCTG	147.51943
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06832_Cmae_Bot2011_COI	T8-17	1	BC06832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	ATGAACAGTCTATCCTCCTTTAG	147.53022
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.647	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06832_Mros_Gib2022_COI	T8-17	1	BC06832_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Present	Sample	Mros_Gib2022_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53022
BC11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83582	WGS84	BC11602	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.111	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11602_Mgig_Bot2012_COI	S10-01	1	BC11602_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11602	Sample	BC11602	targeted	Present	Sample	Mgig_Bot2012_COI	S10-01	COI	45	BC11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.5082
BC11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80898	WGS84	BC11606	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.072	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11606_Mgig_Bot2012_COI	S10-09	1	BC11606_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11606	Sample	BC11606	targeted	Present	Sample	Mgig_Bot2012_COI	S10-09	COI	45	BC11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51707
BC11613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80944	WGS84	BC11613	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.381	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11613_Mgig_Bot2012_COI	S10-08	1	BC11613_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11613	Sample	BC11613	targeted	Present	Sample	Mgig_Bot2012_COI	S10-08	COI	45	BC11613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51811
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.619	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11614_Cmae_Bot2011_COI	T15-16	1	BC11614_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Present	Sample	Cmae_Bot2011_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	ATGAACAGTCTATCCTCCTTTAG	147.53792
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11614_Mros_Gib2022_COI	T15-16	1	BC11614_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Absent	Sample	Mros_Gib2022_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53792
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.817	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11616_Aamu_Bax2006_COI	T16-02	1	BC11616_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Present	Sample	Aamu_Bax2006_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	GCACAACCGGGATCTTTACTTC	147.52991
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11616_Pcan_Bot2011_IGS	T16-02	1	BC11616_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-02	IGS	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	CGTAATCCTCAGTACTGGCTA	147.52991
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11616_Sspa_Oph2007_COI	T16-02	1	BC11616_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	GAAGGCCTTCCGATGATC	147.52991
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11616_Upin_Bot2011_COI	T16-02	1	BC11616_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Absent	Sample	Upin_Bot2011_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.52991
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11617_Cjap_Sim2018_COI	T16-03	1	BC11617_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52225
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.014	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11617_Mgig_Bot2012_COI	T16-03	1	BC11617_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Present	Sample	Mgig_Bot2012_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.52225
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11617_Mjap_Gib2022_COI	T16-03	1	BC11617_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	AATTTAATTGTGACTAGGCATGGTCTG	147.52225
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.415	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11618_Cmae_Bot2011_COI	T16-04	1	BC11618_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Present	Sample	Cmae_Bot2011_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	ATGAACAGTCTATCCTCCTTTAG	147.51987
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	33.541	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11618_Mros_Gib2022_COI	T16-04	1	BC11618_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Present	Sample	Mros_Gib2022_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.51987
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11620_Cjap_Sim2018_COI	T16-06	1	BC11620_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.48517
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11620_Mgig_Bot2012_COI	T16-06	1	BC11620_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Mgig_Bot2012_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.48517
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11620_Mjap_Gib2022_COI	T16-06	1	BC11620_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	AATTTAATTGTGACTAGGCATGGTCTG	147.48517
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.595	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11621_Cmae_Bot2011_COI	T16-07	1	BC11621_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Present	Sample	Cmae_Bot2011_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	ATGAACAGTCTATCCTCCTTTAG	147.50225
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11621_Mros_Gib2022_COI	T16-07	1	BC11621_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Absent	Sample	Mros_Gib2022_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.50225
BC11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83731	WGS84	BC11624	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.948	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11624_Mgig_Bot2012_COI	S11-02	1	BC11624_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11624	Sample	BC11624	targeted	Present	Sample	Mgig_Bot2012_COI	S11-02	COI	45	BC11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.51065
BC11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84678	WGS84	BC11628	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.598	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11628_Mgig_Bot2012_COI	S11-06	1	BC11628_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11628	Sample	BC11628	targeted	Present	Sample	Mgig_Bot2012_COI	S11-06	COI	45	BC11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.49717
BC11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78573	WGS84	BC11631	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.417	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11631_Mgig_Bot2012_COI	S11-09	1	BC11631_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11631	Sample	BC11631	targeted	Present	Sample	Mgig_Bot2012_COI	S11-09	COI	45	BC11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51488
BC11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84717	WGS84	BC11635	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.134	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11635_Mgig_Bot2012_COI	S12-01	1	BC11635_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11635	Sample	BC11635	targeted	Present	Sample	Mgig_Bot2012_COI	S12-01	COI	45	BC11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.49765
BC11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83647	WGS84	BC11639	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.422	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11639_Mgig_Bot2012_COI	S12-05	1	BC11639_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11639	Sample	BC11639	targeted	Present	Sample	Mgig_Bot2012_COI	S12-05	COI	45	BC11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.50935
BC11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78123	WGS84	BC11642	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.507	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11642_Mgig_Bot2012_COI	S12-08	1	BC11642_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11642	Sample	BC11642	targeted	Present	Sample	Mgig_Bot2012_COI	S12-08	COI	45	BC11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51722
BC11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80966	WGS84	BC11646	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.894	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11646_Mgig_Bot2012_COI	S12-12	1	BC11646_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11646	Sample	BC11646	targeted	Present	Sample	Mgig_Bot2012_COI	S12-12	COI	45	BC11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.52121
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	24.807	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11647_Cmae_Bot2011_COI	T17-01	1	BC11647_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Present	Sample	Cmae_Bot2011_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	ATGAACAGTCTATCCTCCTTTAG	147.53883
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	24.457	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11647_Mros_Gib2022_COI	T17-01	1	BC11647_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Present	Sample	Mros_Gib2022_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53883
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.943	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11649_Aamu_Bax2006_COI	T17-04	1	BC11649_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Present	Sample	Aamu_Bax2006_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	GCACAACCGGGATCTTTACTTC	147.52798
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11649_Pcan_Bot2011_IGS	T17-04	1	BC11649_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-04	IGS	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	CGTAATCCTCAGTACTGGCTA	147.52798
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11649_Sspa_Oph2007_COI	T17-04	1	BC11649_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	GAAGGCCTTCCGATGATC	147.52798
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11649_Upin_Bot2011_COI	T17-04	1	BC11649_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Absent	Sample	Upin_Bot2011_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.52798
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.09	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11650_Cmae_Bot2011_COI	T17-05	1	BC11650_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Present	Sample	Cmae_Bot2011_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	ATGAACAGTCTATCCTCCTTTAG	147.47356
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11650_Mros_Gib2022_COI	T17-05	1	BC11650_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Absent	Sample	Mros_Gib2022_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.47356
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.247	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11652_Aamu_Bax2006_COI	T17-08	1	BC11652_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Present	Sample	Aamu_Bax2006_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	GCACAACCGGGATCTTTACTTC	147.52213
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11652_Pcan_Bot2011_IGS	T17-08	1	BC11652_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-08	IGS	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	CGTAATCCTCAGTACTGGCTA	147.52213
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11652_Sspa_Oph2007_COI	T17-08	1	BC11652_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	GAAGGCCTTCCGATGATC	147.52213
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11652_Upin_Bot2011_COI	T17-08	1	BC11652_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Absent	Sample	Upin_Bot2011_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.52213
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11653_Cjap_Sim2018_COI	T17-02	1	BC11653_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52365
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.696	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	11/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11653_Mgig_Bot2012_COI	T17-02	1	BC11653_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Present	Sample	Mgig_Bot2012_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.52365
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11653_Mjap_Gib2022_COI	T17-02	1	BC11653_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	AATTTAATTGTGACTAGGCATGGTCTG	147.52365
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.882	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11654_Cmae_Bot2011_COI	T17-06	1	BC11654_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Present	Sample	Cmae_Bot2011_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	ATGAACAGTCTATCCTCCTTTAG	147.4865
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.048	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11654_Mros_Gib2022_COI	T17-06	1	BC11654_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Present	Sample	Mros_Gib2022_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.4865
BC11657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.77844	WGS84	BC11657	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.234	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11657_Mgig_Bot2012_COI	S9-01	1	BC11657_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11657	Sample	BC11657	targeted	Present	Sample	Mgig_Bot2012_COI	S9-01	COI	45	BC11657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.51846
BC11660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84682	WGS84	BC11660	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11660_Mgig_Bot2012_COI	S9-04	1	BC11660_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11660	Sample	BC11660	targeted	Absent	Sample	Mgig_Bot2012_COI	S9-04	COI	45	BC11660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49734
BC11664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83684	WGS84	BC11664	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11664_Mgig_Bot2012_COI	S9-08	1	BC11664_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11664	Sample	BC11664	targeted	Absent	Sample	Mgig_Bot2012_COI	S9-08	COI	45	BC11664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.5099
BC11667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96492	WGS84	BC11667	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11667_Aamu_Bax2006_COI	T18-09	1	BC11667_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11667	Sample	BC11667	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-09	COI	45	BC11667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-09	GCACAACCGGGATCTTTACTTC	147.51981
BC11667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96492	WGS84	BC11667	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11667_Pcan_Bot2011_IGS	T18-09	1	BC11667_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11667	Sample	BC11667	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-09	IGS	45	BC11667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-09	CGTAATCCTCAGTACTGGCTA	147.51981
BC11667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96492	WGS84	BC11667	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11667_Sspa_Oph2007_COI	T18-09	1	BC11667_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11667	Sample	BC11667	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-09	COI	45	BC11667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-09	GAAGGCCTTCCGATGATC	147.51981
BC11667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96492	WGS84	BC11667	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11667_Upin_Bot2011_COI	T18-09	1	BC11667_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11667	Sample	BC11667	targeted	Absent	Sample	Upin_Bot2011_COI	T18-09	COI	45	BC11667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.51981
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11671_Cjap_Sim2018_COI	T15-15	1	BC11671_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.5266
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.083	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	30/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11671_Mgig_Bot2012_COI	T15-15	1	BC11671_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Present	Sample	Mgig_Bot2012_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.5266
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11671_Mjap_Gib2022_COI	T15-15	1	BC11671_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	AATTTAATTGTGACTAGGCATGGTCTG	147.5266
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.604	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11672_Cmae_Bot2011_COI	T15-14	1	BC11672_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Present	Sample	Cmae_Bot2011_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	ATGAACAGTCTATCCTCCTTTAG	147.51958
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11672_Mros_Gib2022_COI	T15-14	1	BC11672_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Absent	Sample	Mros_Gib2022_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.51958
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.9	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11674_Aamu_Bax2006_COI	T15-12	1	BC11674_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Present	Sample	Aamu_Bax2006_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	GCACAACCGGGATCTTTACTTC	147.33631
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11674_Pcan_Bot2011_IGS	T15-12	1	BC11674_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-12	IGS	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	CGTAATCCTCAGTACTGGCTA	147.33631
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11674_Sspa_Oph2007_COI	T15-12	1	BC11674_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	GAAGGCCTTCCGATGATC	147.33631
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11674_Upin_Bot2011_COI	T15-12	1	BC11674_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Upin_Bot2011_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.33631
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11675_Cjap_Sim2018_COI	T15-11	1	BC11675_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33592
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11675_Mgig_Bot2012_COI	T15-11	1	BC11675_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33592
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11675_Mjap_Gib2022_COI	T15-11	1	BC11675_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	AATTTAATTGTGACTAGGCATGGTCTG	147.33592
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.188	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11676_Cmae_Bot2011_COI	T15-10	1	BC11676_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Present	Sample	Cmae_Bot2011_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	ATGAACAGTCTATCCTCCTTTAG	147.34183
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11676_Mros_Gib2022_COI	T15-10	1	BC11676_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Absent	Sample	Mros_Gib2022_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34183
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.119	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11678_Aamu_Bax2006_COI	T15-08	1	BC11678_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Present	Sample	Aamu_Bax2006_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	GCACAACCGGGATCTTTACTTC	147.33654
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11678_Pcan_Bot2011_IGS	T15-08	1	BC11678_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-08	IGS	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	CGTAATCCTCAGTACTGGCTA	147.33654
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11678_Sspa_Oph2007_COI	T15-08	1	BC11678_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	GAAGGCCTTCCGATGATC	147.33654
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11678_Upin_Bot2011_COI	T15-08	1	BC11678_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Upin_Bot2011_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.33654
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11679_Cjap_Sim2018_COI	T15-07	1	BC11679_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30774
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11679_Mgig_Bot2012_COI	T15-07	1	BC11679_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.30774
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11679_Mjap_Gib2022_COI	T15-07	1	BC11679_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	AATTTAATTGTGACTAGGCATGGTCTG	147.30774
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11681_Aamu_Bax2006_COI	T15-05	1	BC11681_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Aamu_Bax2006_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	GCACAACCGGGATCTTTACTTC	147.30244
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11681_Pcan_Bot2011_IGS	T15-05	1	BC11681_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-05	IGS	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	CGTAATCCTCAGTACTGGCTA	147.30244
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11681_Sspa_Oph2007_COI	T15-05	1	BC11681_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	GAAGGCCTTCCGATGATC	147.30244
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11681_Upin_Bot2011_COI	T15-05	1	BC11681_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Upin_Bot2011_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.30244
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11682_Cjap_Sim2018_COI	T15-04	1	BC11682_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81383
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	24/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11682_Mgig_Bot2012_COI	T15-04	1	BC11682_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.81383
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11682_Mjap_Gib2022_COI	T15-04	1	BC11682_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	AATTTAATTGTGACTAGGCATGGTCTG	147.81383
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11683_Cmae_Bot2011_COI	T15-03	1	BC11683_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	ATGAACAGTCTATCCTCCTTTAG	147.87269
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11683_Mros_Gib2022_COI	T15-03	1	BC11683_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Absent	Sample	Mros_Gib2022_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87269
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.081	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11685_Aamu_Bax2006_COI	T15-01	1	BC11685_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Present	Sample	Aamu_Bax2006_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	GCACAACCGGGATCTTTACTTC	147.87463
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11685_Pcan_Bot2011_IGS	T15-01	1	BC11685_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-01	IGS	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	CGTAATCCTCAGTACTGGCTA	147.87463
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11685_Sspa_Oph2007_COI	T15-01	1	BC11685_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	GAAGGCCTTCCGATGATC	147.87463
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.524	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11685_Upin_Bot2011_COI	T15-01	1	BC11685_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Present	Sample	Upin_Bot2011_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.87463
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11686_Cjap_Sim2018_COI	T14-11	1	BC11686_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34177
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11686_Mgig_Bot2012_COI	T14-11	1	BC11686_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.34177
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11686_Mjap_Gib2022_COI	T14-11	1	BC11686_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	AATTTAATTGTGACTAGGCATGGTCTG	147.34177
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.718	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11687_Cmae_Bot2011_COI	T14-15	1	BC11687_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Present	Sample	Cmae_Bot2011_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	ATGAACAGTCTATCCTCCTTTAG	147.5197
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11687_Mros_Gib2022_COI	T14-15	1	BC11687_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Absent	Sample	Mros_Gib2022_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.5197
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.899	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11689_Aamu_Bax2006_COI	T14-13	1	BC11689_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Present	Sample	Aamu_Bax2006_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	GCACAACCGGGATCTTTACTTC	147.33753
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11689_Pcan_Bot2011_IGS	T14-13	1	BC11689_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-13	IGS	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	CGTAATCCTCAGTACTGGCTA	147.33753
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11689_Sspa_Oph2007_COI	T14-13	1	BC11689_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	GAAGGCCTTCCGATGATC	147.33753
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11689_Upin_Bot2011_COI	T14-13	1	BC11689_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Upin_Bot2011_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.33753
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	40.435	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11690_Cmae_Bot2011_COI	T14-12	1	BC11690_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Present	Sample	Cmae_Bot2011_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	ATGAACAGTCTATCCTCCTTTAG	147.34184
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11690_Mros_Gib2022_COI	T14-12	1	BC11690_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Absent	Sample	Mros_Gib2022_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34184
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11692_Aamu_Bax2006_COI	T14-10	1	BC11692_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Aamu_Bax2006_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	GCACAACCGGGATCTTTACTTC	147.33662
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11692_Pcan_Bot2011_IGS	T14-10	1	BC11692_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-10	IGS	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	CGTAATCCTCAGTACTGGCTA	147.33662
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11692_Sspa_Oph2007_COI	T14-10	1	BC11692_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	GAAGGCCTTCCGATGATC	147.33662
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11692_Upin_Bot2011_COI	T14-10	1	BC11692_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Upin_Bot2011_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.33662
BC11693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88414	WGS84	BC11693	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11693_Mgig_Bot2012_COI	T14-09	1	BC11693_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11693	Sample	BC11693	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-09	COI	45	BC11693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.33655
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	39.608	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11694_Cmae_Bot2011_COI	T14-08	1	BC11694_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Present	Sample	Cmae_Bot2011_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	ATGAACAGTCTATCCTCCTTTAG	147.33681
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11694_Mros_Gib2022_COI	T14-08	1	BC11694_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Absent	Sample	Mros_Gib2022_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33681
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11696_Aamu_Bax2006_COI	T14-06	1	BC11696_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Aamu_Bax2006_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	GCACAACCGGGATCTTTACTTC	147.3019
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11696_Pcan_Bot2011_IGS	T14-06	1	BC11696_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-06	IGS	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	CGTAATCCTCAGTACTGGCTA	147.3019
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11696_Sspa_Oph2007_COI	T14-06	1	BC11696_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	GAAGGCCTTCCGATGATC	147.3019
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11696_Upin_Bot2011_COI	T14-06	1	BC11696_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Upin_Bot2011_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.3019
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11697_Cjap_Sim2018_COI	T14-05	1	BC11697_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30227
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11697_Mgig_Bot2012_COI	T14-05	1	BC11697_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.30227
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11697_Mjap_Gib2022_COI	T14-05	1	BC11697_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	AATTTAATTGTGACTAGGCATGGTCTG	147.30227
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.297	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11698_Cmae_Bot2011_COI	T14-04	1	BC11698_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Present	Sample	Cmae_Bot2011_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	ATGAACAGTCTATCCTCCTTTAG	147.81605
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11698_Mros_Gib2022_COI	T14-04	1	BC11698_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Absent	Sample	Mros_Gib2022_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81605
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.75	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11700_Aamu_Bax2006_COI	T14-02	1	BC11700_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Present	Sample	Aamu_Bax2006_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	GCACAACCGGGATCTTTACTTC	147.87373
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11700_Pcan_Bot2011_IGS	T14-02	1	BC11700_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-02	IGS	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	CGTAATCCTCAGTACTGGCTA	147.87373
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11700_Sspa_Oph2007_COI	T14-02	1	BC11700_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	GAAGGCCTTCCGATGATC	147.87373
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.128	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11700_Upin_Bot2011_COI	T14-02	1	BC11700_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Present	Sample	Upin_Bot2011_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.87373
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11701_Cjap_Sim2018_COI	T14-01	1	BC11701_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87673
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	26/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11701_Mgig_Bot2012_COI	T14-01	1	BC11701_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.87673
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11701_Mjap_Gib2022_COI	T14-01	1	BC11701_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	AATTTAATTGTGACTAGGCATGGTCTG	147.87673
BC15784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88221	WGS84	BC15784	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.343	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15784_Aamu_Bax2006_COI	HA1	0	BC15784_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15784	Sample	BC15784	targeted	Present	Sample	Aamu_Bax2006_COI	HA1	COI	45	BC15784	1.00E-05	Nil	Australia	HA1	GCACAACCGGGATCTTTACTTC	147.34465
BC15784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88221	WGS84	BC15784	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15784_Sspa_Oph2007_COI	HA1	0	BC15784_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15784	Sample	BC15784	targeted	Absent	Sample	Sspa_Oph2007_COI	HA1	COI	45	BC15784	1.00E-05	Nil	Australia	HA1	GAAGGCCTTCCGATGATC	147.34465
BC15784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88221	WGS84	BC15784	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15784_Upin_Bot2011_COI	HA1	0	BC15784_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15784	Sample	BC15784	targeted	Absent	Sample	Upin_Bot2011_COI	HA1	COI	45	BC15784	1.00E-05	Nil	Australia	HA1	CTTTAATTACAGCGTTTTTATTGTTGT	147.34465
BC15785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88364	WGS84	BC15785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15785_Mgig_Bot2012_COI	HA11	0	BC15785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15785	Sample	BC15785	targeted	Absent	Sample	Mgig_Bot2012_COI	HA11	COI	45	BC15785	1.00E-05	Nil	Australia	HA11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33641
BC15786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88289	WGS84	BC15786	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15786_Cmae_Bot2011_COI	HA13	0	BC15786_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15786	Sample	BC15786	targeted	Absent	Sample	Cmae_Bot2011_COI	HA13	COI	45	BC15786	1.00E-05	Nil	Australia	HA13	ATGAACAGTCTATCCTCCTTTAG	147.33818
BC15788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84393	WGS84	BC15788	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.778	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15788_Aamu_Bax2006_COI	HA21	0	BC15788_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15788	Sample	BC15788	targeted	Present	Sample	Aamu_Bax2006_COI	HA21	COI	45	BC15788	1.00E-05	Nil	Australia	HA21	GCACAACCGGGATCTTTACTTC	147.32811
BC15788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84393	WGS84	BC15788	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15788_Sspa_Oph2007_COI	HA21	0	BC15788_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15788	Sample	BC15788	targeted	Absent	Sample	Sspa_Oph2007_COI	HA21	COI	45	BC15788	1.00E-05	Nil	Australia	HA21	GAAGGCCTTCCGATGATC	147.32811
BC15788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84393	WGS84	BC15788	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15788_Upin_Bot2011_COI	HA21	0	BC15788_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15788	Sample	BC15788	targeted	Absent	Sample	Upin_Bot2011_COI	HA21	COI	45	BC15788	1.00E-05	Nil	Australia	HA21	CTTTAATTACAGCGTTTTTATTGTTGT	147.32811
BC15789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83078	WGS84	BC15789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15789_Mgig_Bot2012_COI	HA23	0	BC15789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15789	Sample	BC15789	targeted	Absent	Sample	Mgig_Bot2012_COI	HA23	COI	45	BC15789	1.00E-05	Nil	Australia	HA23	TCTTATTCGTTGGAGACTTTATAACCCT	147.32042
BC15791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18983	WGS84	BC15791	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15791_Aamu_Bax2006_COI	DD2	0	BC15791_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15791	Sample	BC15791	targeted	Absent	Sample	Aamu_Bax2006_COI	DD2	COI	45	BC15791	1.00E-05	Nil	Australia	DD2	GCACAACCGGGATCTTTACTTC	146.36787
BC15791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18983	WGS84	BC15791	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15791_Sspa_Oph2007_COI	DD2	0	BC15791_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15791	Sample	BC15791	targeted	Absent	Sample	Sspa_Oph2007_COI	DD2	COI	45	BC15791	1.00E-05	Nil	Australia	DD2	GAAGGCCTTCCGATGATC	146.36787
BC15791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18983	WGS84	BC15791	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15791_Upin_Bot2011_COI	DD2	0	BC15791_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15791	Sample	BC15791	targeted	Absent	Sample	Upin_Bot2011_COI	DD2	COI	45	BC15791	1.00E-05	Nil	Australia	DD2	CTTTAATTACAGCGTTTTTATTGTTGT	146.36787
BC15792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18675	WGS84	BC15792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15792_Mgig_Bot2012_COI	DD7	0	BC15792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15792	Sample	BC15792	targeted	Absent	Sample	Mgig_Bot2012_COI	DD7	COI	45	BC15792	1.00E-05	Nil	Australia	DD7	TCTTATTCGTTGGAGACTTTATAACCCT	146.36488
BC15793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18467	WGS84	BC15793	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15793_Cmae_Bot2011_COI	DD11	0	BC15793_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15793	Sample	BC15793	targeted	Absent	Sample	Cmae_Bot2011_COI	DD11	COI	45	BC15793	1.00E-05	Nil	Australia	DD11	ATGAACAGTCTATCCTCCTTTAG	146.36495
BC15795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84704	WGS84	BC15795	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.487	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15795_Aamu_Bax2006_COI	MB27	0	BC15795_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15795	Sample	BC15795	targeted	Present	Sample	Aamu_Bax2006_COI	MB27	COI	45	BC15795	1.00E-05	Nil	Australia	MB27	GCACAACCGGGATCTTTACTTC	144.9319
BC15795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84704	WGS84	BC15795	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.191	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15795_Sspa_Oph2007_COI	MB27	0	BC15795_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15795	Sample	BC15795	targeted	Present	Sample	Sspa_Oph2007_COI	MB27	COI	45	BC15795	1.00E-05	Nil	Australia	MB27	GAAGGCCTTCCGATGATC	144.9319
BC15795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84704	WGS84	BC15795	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.046	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15795_Upin_Bot2011_COI	MB27	0	BC15795_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15795	Sample	BC15795	targeted	Present	Sample	Upin_Bot2011_COI	MB27	COI	45	BC15795	1.00E-05	Nil	Australia	MB27	CTTTAATTACAGCGTTTTTATTGTTGT	144.9319
BC15796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8438	WGS84	BC15796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15796_Mgig_Bot2012_COI	MB24	0	BC15796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15796	Sample	BC15796	targeted	Absent	Sample	Mgig_Bot2012_COI	MB24	COI	45	BC15796	1.00E-05	Nil	Australia	MB24	TCTTATTCGTTGGAGACTTTATAACCCT	144.92894
BC15797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84189	WGS84	BC15797	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15797_Cmae_Bot2011_COI	MB21	0	BC15797_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15797	Sample	BC15797	targeted	Absent	Sample	Cmae_Bot2011_COI	MB21	COI	45	BC15797	1.00E-05	Nil	Australia	MB21	ATGAACAGTCTATCCTCCTTTAG	144.92719
BC15799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85028	WGS84	BC15799	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.771	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15799_Aamu_Bax2006_COI	MB17	0	BC15799_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15799	Sample	BC15799	targeted	Present	Sample	Aamu_Bax2006_COI	MB17	COI	45	BC15799	1.00E-05	Nil	Australia	MB17	GCACAACCGGGATCTTTACTTC	144.91393
BC15799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85028	WGS84	BC15799	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.712	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15799_Sspa_Oph2007_COI	MB17	0	BC15799_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15799	Sample	BC15799	targeted	Present	Sample	Sspa_Oph2007_COI	MB17	COI	45	BC15799	1.00E-05	Nil	Australia	MB17	GAAGGCCTTCCGATGATC	144.91393
BC15799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85028	WGS84	BC15799	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.855	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15799_Upin_Bot2011_COI	MB17	0	BC15799_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15799	Sample	BC15799	targeted	Present	Sample	Upin_Bot2011_COI	MB17	COI	45	BC15799	1.00E-05	Nil	Australia	MB17	CTTTAATTACAGCGTTTTTATTGTTGT	144.91393
BC15800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84782	WGS84	BC15800	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15800_Mgig_Bot2012_COI	MB14	0	BC15800_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15800	Sample	BC15800	targeted	Absent	Sample	Mgig_Bot2012_COI	MB14	COI	45	BC15800	1.00E-05	Nil	Australia	MB14	TCTTATTCGTTGGAGACTTTATAACCCT	144.91026
BC15801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82417	WGS84	BC15801	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15801_Cmae_Bot2011_COI	MB123	0	BC15801_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15801	Sample	BC15801	targeted	Absent	Sample	Cmae_Bot2011_COI	MB123	COI	45	BC15801	1.00E-05	Nil	Australia	MB123	ATGAACAGTCTATCCTCCTTTAG	144.9012
BC15803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82012	WGS84	BC15803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15803_Aamu_Bax2006_COI	MB118	0	BC15803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15803	Sample	BC15803	targeted	Absent	Sample	Aamu_Bax2006_COI	MB118	COI	45	BC15803	1.00E-05	Nil	Australia	MB118	GCACAACCGGGATCTTTACTTC	144.90557
BC15803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82012	WGS84	BC15803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.93	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15803_Sspa_Oph2007_COI	MB118	0	BC15803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15803	Sample	BC15803	targeted	Present	Sample	Sspa_Oph2007_COI	MB118	COI	45	BC15803	1.00E-05	Nil	Australia	MB118	GAAGGCCTTCCGATGATC	144.90557
BC15803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82012	WGS84	BC15803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15803_Upin_Bot2011_COI	MB118	0	BC15803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15803	Sample	BC15803	targeted	Absent	Sample	Upin_Bot2011_COI	MB118	COI	45	BC15803	1.00E-05	Nil	Australia	MB118	CTTTAATTACAGCGTTTTTATTGTTGT	144.90557
BC15804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81895	WGS84	BC15804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.598	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15804_Mgig_Bot2012_COI	MB117	0	BC15804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15804	Sample	BC15804	targeted	Present	Sample	Mgig_Bot2012_COI	MB117	COI	45	BC15804	1.00E-05	Nil	Australia	MB117	TCTTATTCGTTGGAGACTTTATAACCCT	144.90584
BC15805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81639	WGS84	BC15805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15805_Cmae_Bot2011_COI	MB114	0	BC15805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15805	Sample	BC15805	targeted	Absent	Sample	Cmae_Bot2011_COI	MB114	COI	45	BC15805	1.00E-05	Nil	Australia	MB114	ATGAACAGTCTATCCTCCTTTAG	144.90628
BC15807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81996	WGS84	BC15807	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15807_Aamu_Bax2006_COI	MB109	0	BC15807_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15807	Sample	BC15807	targeted	Absent	Sample	Aamu_Bax2006_COI	MB109	COI	45	BC15807	1.00E-05	Nil	Australia	MB109	GCACAACCGGGATCTTTACTTC	144.90884
BC15807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81996	WGS84	BC15807	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.241	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15807_Sspa_Oph2007_COI	MB109	0	BC15807_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15807	Sample	BC15807	targeted	Present	Sample	Sspa_Oph2007_COI	MB109	COI	45	BC15807	1.00E-05	Nil	Australia	MB109	GAAGGCCTTCCGATGATC	144.90884
BC15807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81996	WGS84	BC15807	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15807_Upin_Bot2011_COI	MB109	0	BC15807_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15807	Sample	BC15807	targeted	Absent	Sample	Upin_Bot2011_COI	MB109	COI	45	BC15807	1.00E-05	Nil	Australia	MB109	CTTTAATTACAGCGTTTTTATTGTTGT	144.90884
BC15808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81918	WGS84	BC15808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.869	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15808_Mgig_Bot2012_COI	MB107	0	BC15808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15808	Sample	BC15808	targeted	Present	Sample	Mgig_Bot2012_COI	MB107	COI	45	BC15808	1.00E-05	Nil	Australia	MB107	TCTTATTCGTTGGAGACTTTATAACCCT	144.91054
BC15809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81839	WGS84	BC15809	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15809_Cmae_Bot2011_COI	MB106	0	BC15809_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15809	Sample	BC15809	targeted	Absent	Sample	Cmae_Bot2011_COI	MB106	COI	45	BC15809	1.00E-05	Nil	Australia	MB106	ATGAACAGTCTATCCTCCTTTAG	144.91417
BC15810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81528	WGS84	BC15810	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15810_Aamu_Bax2006_COI	MB103	0	BC15810_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15810	Sample	BC15810	targeted	Absent	Sample	Aamu_Bax2006_COI	MB103	COI	45	BC15810	1.00E-05	Nil	Australia	MB103	GCACAACCGGGATCTTTACTTC	144.91289
BC15810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81528	WGS84	BC15810	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.851	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15810_Sspa_Oph2007_COI	MB103	0	BC15810_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15810	Sample	BC15810	targeted	Present	Sample	Sspa_Oph2007_COI	MB103	COI	45	BC15810	1.00E-05	Nil	Australia	MB103	GAAGGCCTTCCGATGATC	144.91289
BC15810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81528	WGS84	BC15810	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	42.432	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15810_Upin_Bot2011_COI	MB103	0	BC15810_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15810	Sample	BC15810	targeted	Present	Sample	Upin_Bot2011_COI	MB103	COI	45	BC15810	1.00E-05	Nil	Australia	MB103	CTTTAATTACAGCGTTTTTATTGTTGT	144.91289
BC15811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8126	WGS84	BC15811	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.306	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15811_Mgig_Bot2012_COI	MB101	0	BC15811_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15811	Sample	BC15811	targeted	Present	Sample	Mgig_Bot2012_COI	MB101	COI	45	BC15811	1.00E-05	Nil	Australia	MB101	TCTTATTCGTTGGAGACTTTATAACCCT	144.91319
BC15812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.83951	WGS84	BC15812	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15812_Cmae_Bot2011_COI	MB1	0	BC15812_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15812	Sample	BC15812	targeted	Absent	Sample	Cmae_Bot2011_COI	MB1	COI	45	BC15812	1.00E-05	Nil	Australia	MB1	ATGAACAGTCTATCCTCCTTTAG	144.90387
BC15815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81832	WGS84	BC15815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15815_Mgig_Bot2012_COI	MA94	0	BC15815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15815	Sample	BC15815	targeted	Absent	Sample	Mgig_Bot2012_COI	MA94	COI	45	BC15815	1.00E-05	Nil	Australia	MA94	TCTTATTCGTTGGAGACTTTATAACCCT	144.91822
BC15816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81807	WGS84	BC15816	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15816_Cmae_Bot2011_COI	MA93	0	BC15816_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15816	Sample	BC15816	targeted	Absent	Sample	Cmae_Bot2011_COI	MA93	COI	45	BC15816	1.00E-05	Nil	Australia	MA93	ATGAACAGTCTATCCTCCTTTAG	144.91519
BC15818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85808	WGS84	BC15818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.963	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15818_Aamu_Bax2006_COI	MA9	0	BC15818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15818	Sample	BC15818	targeted	Present	Sample	Aamu_Bax2006_COI	MA9	COI	45	BC15818	1.00E-05	Nil	Australia	MA9	GCACAACCGGGATCTTTACTTC	144.91
BC15818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85808	WGS84	BC15818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.047	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15818_Sspa_Oph2007_COI	MA9	0	BC15818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15818	Sample	BC15818	targeted	Present	Sample	Sspa_Oph2007_COI	MA9	COI	45	BC15818	1.00E-05	Nil	Australia	MA9	GAAGGCCTTCCGATGATC	144.91
BC15818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85808	WGS84	BC15818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.841	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15818_Upin_Bot2011_COI	MA9	0	BC15818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15818	Sample	BC15818	targeted	Present	Sample	Upin_Bot2011_COI	MA9	COI	45	BC15818	1.00E-05	Nil	Australia	MA9	CTTTAATTACAGCGTTTTTATTGTTGT	144.91
BC15819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81923	WGS84	BC15819	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15819_Mgig_Bot2012_COI	MA89	0	BC15819_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15819	Sample	BC15819	targeted	Absent	Sample	Mgig_Bot2012_COI	MA89	COI	45	BC15819	1.00E-05	Nil	Australia	MA89	TCTTATTCGTTGGAGACTTTATAACCCT	144.91051
BC15821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85992	WGS84	BC15821	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.251	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15821_Aamu_Bax2006_COI	MA84	0	BC15821_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15821	Sample	BC15821	targeted	Present	Sample	Aamu_Bax2006_COI	MA84	COI	45	BC15821	1.00E-05	Nil	Australia	MA84	GCACAACCGGGATCTTTACTTC	144.91472
BC15821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85992	WGS84	BC15821	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.572	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15821_Sspa_Oph2007_COI	MA84	0	BC15821_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15821	Sample	BC15821	targeted	Present	Sample	Sspa_Oph2007_COI	MA84	COI	45	BC15821	1.00E-05	Nil	Australia	MA84	GAAGGCCTTCCGATGATC	144.91472
BC15821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85992	WGS84	BC15821	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.821	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15821_Upin_Bot2011_COI	MA84	0	BC15821_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15821	Sample	BC15821	targeted	Present	Sample	Upin_Bot2011_COI	MA84	COI	45	BC15821	1.00E-05	Nil	Australia	MA84	CTTTAATTACAGCGTTTTTATTGTTGT	144.91472
BC15822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86324	WGS84	BC15822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15822_Mgig_Bot2012_COI	MA83	0	BC15822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15822	Sample	BC15822	targeted	Absent	Sample	Mgig_Bot2012_COI	MA83	COI	45	BC15822	1.00E-05	Nil	Australia	MA83	TCTTATTCGTTGGAGACTTTATAACCCT	144.91424
BC15823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86202	WGS84	BC15823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15823_Cmae_Bot2011_COI	MA82	0	BC15823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15823	Sample	BC15823	targeted	Absent	Sample	Cmae_Bot2011_COI	MA82	COI	45	BC15823	1.00E-05	Nil	Australia	MA82	ATGAACAGTCTATCCTCCTTTAG	144.91645
BC15825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86447	WGS84	BC15825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.28	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15825_Aamu_Bax2006_COI	MA73	0	BC15825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15825	Sample	BC15825	targeted	Present	Sample	Aamu_Bax2006_COI	MA73	COI	45	BC15825	1.00E-05	Nil	Australia	MA73	GCACAACCGGGATCTTTACTTC	144.91705
BC15825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86447	WGS84	BC15825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.713	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15825_Sspa_Oph2007_COI	MA73	0	BC15825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15825	Sample	BC15825	targeted	Present	Sample	Sspa_Oph2007_COI	MA73	COI	45	BC15825	1.00E-05	Nil	Australia	MA73	GAAGGCCTTCCGATGATC	144.91705
BC15825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86447	WGS84	BC15825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	28.458	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15825_Upin_Bot2011_COI	MA73	0	BC15825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15825	Sample	BC15825	targeted	Present	Sample	Upin_Bot2011_COI	MA73	COI	45	BC15825	1.00E-05	Nil	Australia	MA73	CTTTAATTACAGCGTTTTTATTGTTGT	144.91705
BC15827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86208	WGS84	BC15827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15827_Cmae_Bot2011_COI	MA67	0	BC15827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15827	Sample	BC15827	targeted	Absent	Sample	Cmae_Bot2011_COI	MA67	COI	45	BC15827	1.00E-05	Nil	Australia	MA67	ATGAACAGTCTATCCTCCTTTAG	144.91525
BC15830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8263	WGS84	BC15830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15830_Cmae_Bot2011_COI	MA58	0	BC15830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15830	Sample	BC15830	targeted	Absent	Sample	Cmae_Bot2011_COI	MA58	COI	45	BC15830	1.00E-05	Nil	Australia	MA58	ATGAACAGTCTATCCTCCTTTAG	144.90263
BC15832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8201	WGS84	BC15832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.537	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15832_Aamu_Bax2006_COI	MA56	0	BC15832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15832	Sample	BC15832	targeted	Present	Sample	Aamu_Bax2006_COI	MA56	COI	45	BC15832	1.00E-05	Nil	Australia	MA56	GCACAACCGGGATCTTTACTTC	144.91851
BC15832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8201	WGS84	BC15832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15832_Sspa_Oph2007_COI	MA56	0	BC15832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15832	Sample	BC15832	targeted	Absent	Sample	Sspa_Oph2007_COI	MA56	COI	45	BC15832	1.00E-05	Nil	Australia	MA56	GAAGGCCTTCCGATGATC	144.91851
BC15832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8201	WGS84	BC15832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15832_Upin_Bot2011_COI	MA56	0	BC15832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15832	Sample	BC15832	targeted	Absent	Sample	Upin_Bot2011_COI	MA56	COI	45	BC15832	1.00E-05	Nil	Australia	MA56	CTTTAATTACAGCGTTTTTATTGTTGT	144.91851
BC15833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81978	WGS84	BC15833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15833_Mgig_Bot2012_COI	MA52	0	BC15833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15833	Sample	BC15833	targeted	Absent	Sample	Mgig_Bot2012_COI	MA52	COI	45	BC15833	1.00E-05	Nil	Australia	MA52	TCTTATTCGTTGGAGACTTTATAACCCT	144.92565
BC15834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81754	WGS84	BC15834	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.351	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15834_Cmae_Bot2011_COI	MA46	0	BC15834_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15834	Sample	BC15834	targeted	Present	Sample	Cmae_Bot2011_COI	MA46	COI	45	BC15834	1.00E-05	Nil	Australia	MA46	ATGAACAGTCTATCCTCCTTTAG	144.92417
BC15836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8645	WGS84	BC15836	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.521	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15836_Aamu_Bax2006_COI	MA42	0	BC15836_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15836	Sample	BC15836	targeted	Present	Sample	Aamu_Bax2006_COI	MA42	COI	45	BC15836	1.00E-05	Nil	Australia	MA42	GCACAACCGGGATCTTTACTTC	144.9176
BC15836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8645	WGS84	BC15836	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.906	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15836_Sspa_Oph2007_COI	MA42	0	BC15836_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15836	Sample	BC15836	targeted	Present	Sample	Sspa_Oph2007_COI	MA42	COI	45	BC15836	1.00E-05	Nil	Australia	MA42	GAAGGCCTTCCGATGATC	144.9176
BC15836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8645	WGS84	BC15836	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.595	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15836_Upin_Bot2011_COI	MA42	0	BC15836_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15836	Sample	BC15836	targeted	Present	Sample	Upin_Bot2011_COI	MA42	COI	45	BC15836	1.00E-05	Nil	Australia	MA42	CTTTAATTACAGCGTTTTTATTGTTGT	144.9176
BC15837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81973	WGS84	BC15837	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15837_Mgig_Bot2012_COI	MA4	0	BC15837_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15837	Sample	BC15837	targeted	Absent	Sample	Mgig_Bot2012_COI	MA4	COI	45	BC15837	1.00E-05	Nil	Australia	MA4	TCTTATTCGTTGGAGACTTTATAACCCT	144.92989
BC15838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84279	WGS84	BC15838	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15838_Cmae_Bot2011_COI	MA37	0	BC15838_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15838	Sample	BC15838	targeted	Absent	Sample	Cmae_Bot2011_COI	MA37	COI	45	BC15838	1.00E-05	Nil	Australia	MA37	ATGAACAGTCTATCCTCCTTTAG	144.90429
BC15840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84748	WGS84	BC15840	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15840_Mgig_Bot2012_COI	MA34	0	BC15840_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15840	Sample	BC15840	targeted	Absent	Sample	Mgig_Bot2012_COI	MA34	COI	45	BC15840	1.00E-05	Nil	Australia	MA34	TCTTATTCGTTGGAGACTTTATAACCCT	144.90998
BC15841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84667	WGS84	BC15841	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15841_Cmae_Bot2011_COI	MA31	0	BC15841_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15841	Sample	BC15841	targeted	Absent	Sample	Cmae_Bot2011_COI	MA31	COI	45	BC15841	1.00E-05	Nil	Australia	MA31	ATGAACAGTCTATCCTCCTTTAG	144.90582
BC15843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84537	WGS84	BC15843	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.022	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15843_Aamu_Bax2006_COI	MA29	0	BC15843_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15843	Sample	BC15843	targeted	Present	Sample	Aamu_Bax2006_COI	MA29	COI	45	BC15843	1.00E-05	Nil	Australia	MA29	GCACAACCGGGATCTTTACTTC	144.9053
BC15843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84537	WGS84	BC15843	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.733	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15843_Sspa_Oph2007_COI	MA29	0	BC15843_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15843	Sample	BC15843	targeted	Present	Sample	Sspa_Oph2007_COI	MA29	COI	45	BC15843	1.00E-05	Nil	Australia	MA29	GAAGGCCTTCCGATGATC	144.9053
BC15843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84537	WGS84	BC15843	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.359	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15843_Upin_Bot2011_COI	MA29	0	BC15843_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15843	Sample	BC15843	targeted	Present	Sample	Upin_Bot2011_COI	MA29	COI	45	BC15843	1.00E-05	Nil	Australia	MA29	CTTTAATTACAGCGTTTTTATTGTTGT	144.9053
BC15844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84362	WGS84	BC15844	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15844_Mgig_Bot2012_COI	MA28	0	BC15844_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15844	Sample	BC15844	targeted	Absent	Sample	Mgig_Bot2012_COI	MA28	COI	45	BC15844	1.00E-05	Nil	Australia	MA28	TCTTATTCGTTGGAGACTTTATAACCCT	144.90725
BC15845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84523	WGS84	BC15845	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15845_Cmae_Bot2011_COI	MA23	0	BC15845_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15845	Sample	BC15845	targeted	Absent	Sample	Cmae_Bot2011_COI	MA23	COI	45	BC15845	1.00E-05	Nil	Australia	MA23	ATGAACAGTCTATCCTCCTTTAG	144.90766
BC15847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84461	WGS84	BC15847	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.165	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15847_Aamu_Bax2006_COI	MA123	0	BC15847_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15847	Sample	BC15847	targeted	Present	Sample	Aamu_Bax2006_COI	MA123	COI	45	BC15847	1.00E-05	Nil	Australia	MA123	GCACAACCGGGATCTTTACTTC	144.92611
BC15847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84461	WGS84	BC15847	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.722	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15847_Sspa_Oph2007_COI	MA123	0	BC15847_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15847	Sample	BC15847	targeted	Present	Sample	Sspa_Oph2007_COI	MA123	COI	45	BC15847	1.00E-05	Nil	Australia	MA123	GAAGGCCTTCCGATGATC	144.92611
BC15847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84461	WGS84	BC15847	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.319	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15847_Upin_Bot2011_COI	MA123	0	BC15847_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15847	Sample	BC15847	targeted	Present	Sample	Upin_Bot2011_COI	MA123	COI	45	BC15847	1.00E-05	Nil	Australia	MA123	CTTTAATTACAGCGTTTTTATTGTTGT	144.92611
BC15848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8601	WGS84	BC15848	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15848_Mgig_Bot2012_COI	MA122	0	BC15848_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15848	Sample	BC15848	targeted	Absent	Sample	Mgig_Bot2012_COI	MA122	COI	45	BC15848	1.00E-05	Nil	Australia	MA122	TCTTATTCGTTGGAGACTTTATAACCCT	144.91211
BC15849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86282	WGS84	BC15849	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15849_Cmae_Bot2011_COI	MA12	0	BC15849_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15849	Sample	BC15849	targeted	Absent	Sample	Cmae_Bot2011_COI	MA12	COI	45	BC15849	1.00E-05	Nil	Australia	MA12	ATGAACAGTCTATCCTCCTTTAG	144.91377
BC15850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8436	WGS84	BC15850	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.475	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15850_Aamu_Bax2006_COI	MA114	0	BC15850_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15850	Sample	BC15850	targeted	Present	Sample	Aamu_Bax2006_COI	MA114	COI	45	BC15850	1.00E-05	Nil	Australia	MA114	GCACAACCGGGATCTTTACTTC	144.93102
BC15850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8436	WGS84	BC15850	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.739	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15850_Sspa_Oph2007_COI	MA114	0	BC15850_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15850	Sample	BC15850	targeted	Present	Sample	Sspa_Oph2007_COI	MA114	COI	45	BC15850	1.00E-05	Nil	Australia	MA114	GAAGGCCTTCCGATGATC	144.93102
BC15850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8436	WGS84	BC15850	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.216	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15850_Upin_Bot2011_COI	MA114	0	BC15850_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15850	Sample	BC15850	targeted	Present	Sample	Upin_Bot2011_COI	MA114	COI	45	BC15850	1.00E-05	Nil	Australia	MA114	CTTTAATTACAGCGTTTTTATTGTTGT	144.93102
BC15851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82357	WGS84	BC15851	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.194	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15851_Mgig_Bot2012_COI	MA112	0	BC15851_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15851	Sample	BC15851	targeted	Present	Sample	Mgig_Bot2012_COI	MA112	COI	45	BC15851	1.00E-05	Nil	Australia	MA112	TCTTATTCGTTGGAGACTTTATAACCCT	144.90177
BC15852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84545	WGS84	BC15852	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15852_Cmae_Bot2011_COI	MA111	0	BC15852_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15852	Sample	BC15852	targeted	Absent	Sample	Cmae_Bot2011_COI	MA111	COI	45	BC15852	1.00E-05	Nil	Australia	MA111	ATGAACAGTCTATCCTCCTTTAG	144.93045
BC15854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8183	WGS84	BC15854	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.181	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15854_Aamu_Bax2006_COI	MA103	0	BC15854_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15854	Sample	BC15854	targeted	Present	Sample	Aamu_Bax2006_COI	MA103	COI	45	BC15854	1.00E-05	Nil	Australia	MA103	GCACAACCGGGATCTTTACTTC	144.92655
BC15854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8183	WGS84	BC15854	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.893	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15854_Sspa_Oph2007_COI	MA103	0	BC15854_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15854	Sample	BC15854	targeted	Present	Sample	Sspa_Oph2007_COI	MA103	COI	45	BC15854	1.00E-05	Nil	Australia	MA103	GAAGGCCTTCCGATGATC	144.92655
BC15854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8183	WGS84	BC15854	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15854_Upin_Bot2011_COI	MA103	0	BC15854_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15854	Sample	BC15854	targeted	Absent	Sample	Upin_Bot2011_COI	MA103	COI	45	BC15854	1.00E-05	Nil	Australia	MA103	CTTTAATTACAGCGTTTTTATTGTTGT	144.92655
BC15855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82107	WGS84	BC15855	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15855_Mgig_Bot2012_COI	MA102	0	BC15855_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15855	Sample	BC15855	targeted	Absent	Sample	Mgig_Bot2012_COI	MA102	COI	45	BC15855	1.00E-05	Nil	Australia	MA102	TCTTATTCGTTGGAGACTTTATAACCCT	144.9053
BC15856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84654	WGS84	BC15856	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15856_Cmae_Bot2011_COI	MA101	0	BC15856_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15856	Sample	BC15856	targeted	Absent	Sample	Cmae_Bot2011_COI	MA101	COI	45	BC15856	1.00E-05	Nil	Australia	MA101	ATGAACAGTCTATCCTCCTTTAG	144.93095
BC15858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8768	WGS84	BC15858	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.057	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	15/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15858_Aamu_Bax2006_COI	HD12	0	BC15858_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15858	Sample	BC15858	targeted	Present	Sample	Aamu_Bax2006_COI	HD12	COI	45	BC15858	1.00E-05	Nil	Australia	HD12	GCACAACCGGGATCTTTACTTC	147.34188
BC15858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8768	WGS84	BC15858	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	15/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15858_Sspa_Oph2007_COI	HD12	0	BC15858_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15858	Sample	BC15858	targeted	Absent	Sample	Sspa_Oph2007_COI	HD12	COI	45	BC15858	1.00E-05	Nil	Australia	HD12	GAAGGCCTTCCGATGATC	147.34188
BC15858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8768	WGS84	BC15858	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	15/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15858_Upin_Bot2011_COI	HD12	0	BC15858_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15858	Sample	BC15858	targeted	Absent	Sample	Upin_Bot2011_COI	HD12	COI	45	BC15858	1.00E-05	Nil	Australia	HD12	CTTTAATTACAGCGTTTTTATTGTTGT	147.34188
BC15859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83088	WGS84	BC15859	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15859_Mgig_Bot2012_COI	HD1	0	BC15859_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15859	Sample	BC15859	targeted	Absent	Sample	Mgig_Bot2012_COI	HD1	COI	45	BC15859	1.00E-05	Nil	Australia	HD1	TCTTATTCGTTGGAGACTTTATAACCCT	147.32074
BC15861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8434	WGS84	BC15861	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	40.005	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15861_Aamu_Bax2006_COI	HC6	0	BC15861_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15861	Sample	BC15861	targeted	Present	Sample	Aamu_Bax2006_COI	HC6	COI	45	BC15861	1.00E-05	Nil	Australia	HC6	GCACAACCGGGATCTTTACTTC	147.3301
BC15861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8434	WGS84	BC15861	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15861_Sspa_Oph2007_COI	HC6	0	BC15861_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15861	Sample	BC15861	targeted	Absent	Sample	Sspa_Oph2007_COI	HC6	COI	45	BC15861	1.00E-05	Nil	Australia	HC6	GAAGGCCTTCCGATGATC	147.3301
BC15861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8434	WGS84	BC15861	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15861_Upin_Bot2011_COI	HC6	0	BC15861_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15861	Sample	BC15861	targeted	Absent	Sample	Upin_Bot2011_COI	HC6	COI	45	BC15861	1.00E-05	Nil	Australia	HC6	CTTTAATTACAGCGTTTTTATTGTTGT	147.3301
BC15862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.873	WGS84	BC15862	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.858	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15862_Mgig_Bot2012_COI	HC24	0	BC15862_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15862	Sample	BC15862	targeted	Present	Sample	Mgig_Bot2012_COI	HC24	COI	45	BC15862	1.00E-05	Nil	Australia	HC24	TCTTATTCGTTGGAGACTTTATAACCCT	147.3366
BC15863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8759	WGS84	BC15863	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15863_Cmae_Bot2011_COI	HC21	0	BC15863_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15863	Sample	BC15863	targeted	Absent	Sample	Cmae_Bot2011_COI	HC21	COI	45	BC15863	1.00E-05	Nil	Australia	HC21	ATGAACAGTCTATCCTCCTTTAG	147.3413
BC15865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8802	WGS84	BC15865	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15865_Aamu_Bax2006_COI	HC18	0	BC15865_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15865	Sample	BC15865	targeted	Absent	Sample	Aamu_Bax2006_COI	HC18	COI	45	BC15865	1.00E-05	Nil	Australia	HC18	GCACAACCGGGATCTTTACTTC	147.3419
BC15865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8802	WGS84	BC15865	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15865_Sspa_Oph2007_COI	HC18	0	BC15865_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15865	Sample	BC15865	targeted	Absent	Sample	Sspa_Oph2007_COI	HC18	COI	45	BC15865	1.00E-05	Nil	Australia	HC18	GAAGGCCTTCCGATGATC	147.3419
BC15865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8802	WGS84	BC15865	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15865_Upin_Bot2011_COI	HC18	0	BC15865_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15865	Sample	BC15865	targeted	Absent	Sample	Upin_Bot2011_COI	HC18	COI	45	BC15865	1.00E-05	Nil	Australia	HC18	CTTTAATTACAGCGTTTTTATTGTTGT	147.3419
BC15866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8839	WGS84	BC15866	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.91	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15866_Mgig_Bot2012_COI	HC14	0	BC15866_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15866	Sample	BC15866	targeted	Present	Sample	Mgig_Bot2012_COI	HC14	COI	45	BC15866	1.00E-05	Nil	Australia	HC14	TCTTATTCGTTGGAGACTTTATAACCCT	147.3365
BC15867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88525	WGS84	BC15867	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15867_Cmae_Bot2011_COI	HB4	0	BC15867_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15867	Sample	BC15867	targeted	Absent	Sample	Cmae_Bot2011_COI	HB4	COI	45	BC15867	1.00E-05	Nil	Australia	HB4	ATGAACAGTCTATCCTCCTTTAG	147.33569
BC15869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.82939	WGS84	BC15869	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.993	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15869_Aamu_Bax2006_COI	HB23	0	BC15869_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15869	Sample	BC15869	targeted	Present	Sample	Aamu_Bax2006_COI	HB23	COI	45	BC15869	1.00E-05	Nil	Australia	HB23	GCACAACCGGGATCTTTACTTC	147.31869
BC15869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.82939	WGS84	BC15869	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15869_Sspa_Oph2007_COI	HB23	0	BC15869_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15869	Sample	BC15869	targeted	Absent	Sample	Sspa_Oph2007_COI	HB23	COI	45	BC15869	1.00E-05	Nil	Australia	HB23	GAAGGCCTTCCGATGATC	147.31869
BC15869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.82939	WGS84	BC15869	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15869_Upin_Bot2011_COI	HB23	0	BC15869_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15869	Sample	BC15869	targeted	Absent	Sample	Upin_Bot2011_COI	HB23	COI	45	BC15869	1.00E-05	Nil	Australia	HB23	CTTTAATTACAGCGTTTTTATTGTTGT	147.31869
BC15870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88582	WGS84	BC15870	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15870_Cmae_Bot2011_COI	HB2	0	BC15870_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15870	Sample	BC15870	targeted	Absent	Sample	Cmae_Bot2011_COI	HB2	COI	45	BC15870	1.00E-05	Nil	Australia	HB2	ATGAACAGTCTATCCTCCTTTAG	147.33562
BC15872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84554	WGS84	BC15872	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.702	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15872_Aamu_Bax2006_COI	HB17	0	BC15872_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15872	Sample	BC15872	targeted	Present	Sample	Aamu_Bax2006_COI	HB17	COI	45	BC15872	1.00E-05	Nil	Australia	HB17	GCACAACCGGGATCTTTACTTC	147.32933
BC15872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84554	WGS84	BC15872	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15872_Sspa_Oph2007_COI	HB17	0	BC15872_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15872	Sample	BC15872	targeted	Absent	Sample	Sspa_Oph2007_COI	HB17	COI	45	BC15872	1.00E-05	Nil	Australia	HB17	GAAGGCCTTCCGATGATC	147.32933
BC15872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84554	WGS84	BC15872	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15872_Upin_Bot2011_COI	HB17	0	BC15872_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15872	Sample	BC15872	targeted	Absent	Sample	Upin_Bot2011_COI	HB17	COI	45	BC15872	1.00E-05	Nil	Australia	HB17	CTTTAATTACAGCGTTTTTATTGTTGT	147.32933
BC15873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88523	WGS84	BC15873	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15873_Mgig_Bot2012_COI	HB12	0	BC15873_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15873	Sample	BC15873	targeted	Absent	Sample	Mgig_Bot2012_COI	HB12	COI	45	BC15873	1.00E-05	Nil	Australia	HB12	TCTTATTCGTTGGAGACTTTATAACCCT	147.34082
BC15874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88666	WGS84	BC15874	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15874_Cmae_Bot2011_COI	HB11	0	BC15874_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15874	Sample	BC15874	targeted	Absent	Sample	Cmae_Bot2011_COI	HB11	COI	45	BC15874	1.00E-05	Nil	Australia	HB11	ATGAACAGTCTATCCTCCTTTAG	147.33999
BC15876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81962	WGS84	BC15876	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.66	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15876_Aamu_Bax2006_COI	MD99	0	BC15876_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15876	Sample	BC15876	targeted	Present	Sample	Aamu_Bax2006_COI	MD99	COI	45	BC15876	1.00E-05	Nil	Australia	MD99	GCACAACCGGGATCTTTACTTC	144.91304
BC15876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81962	WGS84	BC15876	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.502	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15876_Sspa_Oph2007_COI	MD99	0	BC15876_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15876	Sample	BC15876	targeted	Present	Sample	Sspa_Oph2007_COI	MD99	COI	45	BC15876	1.00E-05	Nil	Australia	MD99	GAAGGCCTTCCGATGATC	144.91304
BC15876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81962	WGS84	BC15876	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15876_Upin_Bot2011_COI	MD99	0	BC15876_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15876	Sample	BC15876	targeted	Absent	Sample	Upin_Bot2011_COI	MD99	COI	45	BC15876	1.00E-05	Nil	Australia	MD99	CTTTAATTACAGCGTTTTTATTGTTGT	144.91304
BC15877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82039	WGS84	BC15877	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15877_Mgig_Bot2012_COI	MD97	0	BC15877_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15877	Sample	BC15877	targeted	Absent	Sample	Mgig_Bot2012_COI	MD97	COI	45	BC15877	1.00E-05	Nil	Australia	MD97	TCTTATTCGTTGGAGACTTTATAACCCT	144.91577
BC15878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81277	WGS84	BC15878	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15878_Cmae_Bot2011_COI	MD92	0	BC15878_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15878	Sample	BC15878	targeted	Absent	Sample	Cmae_Bot2011_COI	MD92	COI	45	BC15878	1.00E-05	Nil	Australia	MD92	ATGAACAGTCTATCCTCCTTTAG	144.91297
BC15880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81641	WGS84	BC15880	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15880_Mgig_Bot2012_COI	MD87	0	BC15880_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15880	Sample	BC15880	targeted	Absent	Sample	Mgig_Bot2012_COI	MD87	COI	45	BC15880	1.00E-05	Nil	Australia	MD87	TCTTATTCGTTGGAGACTTTATAACCCT	144.91328
BC15881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81912	WGS84	BC15881	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.016	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15881_Cmae_Bot2011_COI	MD84	0	BC15881_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15881	Sample	BC15881	targeted	Present	Sample	Cmae_Bot2011_COI	MD84	COI	45	BC15881	1.00E-05	Nil	Australia	MD84	ATGAACAGTCTATCCTCCTTTAG	144.9153
BC15883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8428	WGS84	BC15883	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.702	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15883_Aamu_Bax2006_COI	MD8	0	BC15883_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15883	Sample	BC15883	targeted	Present	Sample	Aamu_Bax2006_COI	MD8	COI	45	BC15883	1.00E-05	Nil	Australia	MD8	GCACAACCGGGATCTTTACTTC	144.92954
BC15883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8428	WGS84	BC15883	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.468	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15883_Sspa_Oph2007_COI	MD8	0	BC15883_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15883	Sample	BC15883	targeted	Present	Sample	Sspa_Oph2007_COI	MD8	COI	45	BC15883	1.00E-05	Nil	Australia	MD8	GAAGGCCTTCCGATGATC	144.92954
BC15883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8428	WGS84	BC15883	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.966	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15883_Upin_Bot2011_COI	MD8	0	BC15883_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15883	Sample	BC15883	targeted	Present	Sample	Upin_Bot2011_COI	MD8	COI	45	BC15883	1.00E-05	Nil	Australia	MD8	CTTTAATTACAGCGTTTTTATTGTTGT	144.92954
BC15884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81769	WGS84	BC15884	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15884_Mgig_Bot2012_COI	MD78	0	BC15884_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15884	Sample	BC15884	targeted	Absent	Sample	Mgig_Bot2012_COI	MD78	COI	45	BC15884	1.00E-05	Nil	Australia	MD78	TCTTATTCGTTGGAGACTTTATAACCCT	144.92064
BC15885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81826	WGS84	BC15885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15885_Cmae_Bot2011_COI	MD76	0	BC15885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15885	Sample	BC15885	targeted	Absent	Sample	Cmae_Bot2011_COI	MD76	COI	45	BC15885	1.00E-05	Nil	Australia	MD76	ATGAACAGTCTATCCTCCTTTAG	144.92264
BC15887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81794	WGS84	BC15887	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.621	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15887_Aamu_Bax2006_COI	MD69	0	BC15887_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15887	Sample	BC15887	targeted	Present	Sample	Aamu_Bax2006_COI	MD69	COI	45	BC15887	1.00E-05	Nil	Australia	MD69	GCACAACCGGGATCTTTACTTC	144.92451
BC15887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81794	WGS84	BC15887	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.184	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15887_Sspa_Oph2007_COI	MD69	0	BC15887_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15887	Sample	BC15887	targeted	Present	Sample	Sspa_Oph2007_COI	MD69	COI	45	BC15887	1.00E-05	Nil	Australia	MD69	GAAGGCCTTCCGATGATC	144.92451
BC15887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81794	WGS84	BC15887	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15887_Upin_Bot2011_COI	MD69	0	BC15887_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15887	Sample	BC15887	targeted	Absent	Sample	Upin_Bot2011_COI	MD69	COI	45	BC15887	1.00E-05	Nil	Australia	MD69	CTTTAATTACAGCGTTTTTATTGTTGT	144.92451
BC15888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81643	WGS84	BC15888	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15888_Mgig_Bot2012_COI	MD67	0	BC15888_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15888	Sample	BC15888	targeted	Absent	Sample	Mgig_Bot2012_COI	MD67	COI	45	BC15888	1.00E-05	Nil	Australia	MD67	TCTTATTCGTTGGAGACTTTATAACCCT	144.92564
BC15889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81561	WGS84	BC15889	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15889_Cmae_Bot2011_COI	MD66	0	BC15889_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15889	Sample	BC15889	targeted	Absent	Sample	Cmae_Bot2011_COI	MD66	COI	45	BC15889	1.00E-05	Nil	Australia	MD66	ATGAACAGTCTATCCTCCTTTAG	144.92688
BC15890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81966	WGS84	BC15890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.626	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15890_Aamu_Bax2006_COI	MD64	0	BC15890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15890	Sample	BC15890	targeted	Present	Sample	Aamu_Bax2006_COI	MD64	COI	45	BC15890	1.00E-05	Nil	Australia	MD64	GCACAACCGGGATCTTTACTTC	144.92612
BC15890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81966	WGS84	BC15890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.337	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15890_Sspa_Oph2007_COI	MD64	0	BC15890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15890	Sample	BC15890	targeted	Present	Sample	Sspa_Oph2007_COI	MD64	COI	45	BC15890	1.00E-05	Nil	Australia	MD64	GAAGGCCTTCCGATGATC	144.92612
BC15890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81966	WGS84	BC15890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15890_Upin_Bot2011_COI	MD64	0	BC15890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15890	Sample	BC15890	targeted	Absent	Sample	Upin_Bot2011_COI	MD64	COI	45	BC15890	1.00E-05	Nil	Australia	MD64	CTTTAATTACAGCGTTTTTATTGTTGT	144.92612
BC15891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81838	WGS84	BC15891	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15891_Mgig_Bot2012_COI	MD61	0	BC15891_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15891	Sample	BC15891	targeted	Absent	Sample	Mgig_Bot2012_COI	MD61	COI	45	BC15891	1.00E-05	Nil	Australia	MD61	TCTTATTCGTTGGAGACTTTATAACCCT	144.92872
BC15892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81935	WGS84	BC15892	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15892_Cmae_Bot2011_COI	MD58	0	BC15892_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15892	Sample	BC15892	targeted	Absent	Sample	Cmae_Bot2011_COI	MD58	COI	45	BC15892	1.00E-05	Nil	Australia	MD58	ATGAACAGTCTATCCTCCTTTAG	144.92941
BC15894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86038	WGS84	BC15894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.139	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15894_Aamu_Bax2006_COI	MD54	0	BC15894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15894	Sample	BC15894	targeted	Present	Sample	Aamu_Bax2006_COI	MD54	COI	45	BC15894	1.00E-05	Nil	Australia	MD54	GCACAACCGGGATCTTTACTTC	144.90903
BC15894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86038	WGS84	BC15894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.769	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15894_Sspa_Oph2007_COI	MD54	0	BC15894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15894	Sample	BC15894	targeted	Present	Sample	Sspa_Oph2007_COI	MD54	COI	45	BC15894	1.00E-05	Nil	Australia	MD54	GAAGGCCTTCCGATGATC	144.90903
BC15894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86038	WGS84	BC15894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.508	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15894_Upin_Bot2011_COI	MD54	0	BC15894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15894	Sample	BC15894	targeted	Present	Sample	Upin_Bot2011_COI	MD54	COI	45	BC15894	1.00E-05	Nil	Australia	MD54	CTTTAATTACAGCGTTTTTATTGTTGT	144.90903
BC15895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85976	WGS84	BC15895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15895_Mgig_Bot2012_COI	MD52	0	BC15895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15895	Sample	BC15895	targeted	Absent	Sample	Mgig_Bot2012_COI	MD52	COI	45	BC15895	1.00E-05	Nil	Australia	MD52	TCTTATTCGTTGGAGACTTTATAACCCT	144.91078
BC15896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86205	WGS84	BC15896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15896_Cmae_Bot2011_COI	MD47	0	BC15896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15896	Sample	BC15896	targeted	Absent	Sample	Cmae_Bot2011_COI	MD47	COI	45	BC15896	1.00E-05	Nil	Australia	MD47	ATGAACAGTCTATCCTCCTTTAG	144.91494
BC15898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86314	WGS84	BC15898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.041	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15898_Aamu_Bax2006_COI	MD42	0	BC15898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15898	Sample	BC15898	targeted	Present	Sample	Aamu_Bax2006_COI	MD42	COI	45	BC15898	1.00E-05	Nil	Australia	MD42	GCACAACCGGGATCTTTACTTC	144.91721
BC15898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86314	WGS84	BC15898	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.132	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15898_Sspa_Oph2007_COI	MD42	0	BC15898_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15898	Sample	BC15898	targeted	Present	Sample	Sspa_Oph2007_COI	MD42	COI	45	BC15898	1.00E-05	Nil	Australia	MD42	GAAGGCCTTCCGATGATC	144.91721
BC15898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86314	WGS84	BC15898	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.484	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15898_Upin_Bot2011_COI	MD42	0	BC15898_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15898	Sample	BC15898	targeted	Present	Sample	Upin_Bot2011_COI	MD42	COI	45	BC15898	1.00E-05	Nil	Australia	MD42	CTTTAATTACAGCGTTTTTATTGTTGT	144.91721
BC15899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86256	WGS84	BC15899	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15899_Mgig_Bot2012_COI	MD41	0	BC15899_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15899	Sample	BC15899	targeted	Absent	Sample	Mgig_Bot2012_COI	MD41	COI	45	BC15899	1.00E-05	Nil	Australia	MD41	TCTTATTCGTTGGAGACTTTATAACCCT	144.91611
BC15900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86111	WGS84	BC15900	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.681	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15900_Cmae_Bot2011_COI	MD34	0	BC15900_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15900	Sample	BC15900	targeted	Present	Sample	Cmae_Bot2011_COI	MD34	COI	45	BC15900	1.00E-05	Nil	Australia	MD34	ATGAACAGTCTATCCTCCTTTAG	144.90524
BC15902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86511	WGS84	BC15902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.534	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15902_Aamu_Bax2006_COI	MD31	0	BC15902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15902	Sample	BC15902	targeted	Present	Sample	Aamu_Bax2006_COI	MD31	COI	45	BC15902	1.00E-05	Nil	Australia	MD31	GCACAACCGGGATCTTTACTTC	144.91823
BC15902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86511	WGS84	BC15902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.221	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15902_Sspa_Oph2007_COI	MD31	0	BC15902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15902	Sample	BC15902	targeted	Present	Sample	Sspa_Oph2007_COI	MD31	COI	45	BC15902	1.00E-05	Nil	Australia	MD31	GAAGGCCTTCCGATGATC	144.91823
BC15902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86511	WGS84	BC15902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.488	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15902_Upin_Bot2011_COI	MD31	0	BC15902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15902	Sample	BC15902	targeted	Present	Sample	Upin_Bot2011_COI	MD31	COI	45	BC15902	1.00E-05	Nil	Australia	MD31	CTTTAATTACAGCGTTTTTATTGTTGT	144.91823
BC15903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86574	WGS84	BC15903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15903_Mgig_Bot2012_COI	MD29	0	BC15903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15903	Sample	BC15903	targeted	Absent	Sample	Mgig_Bot2012_COI	MD29	COI	45	BC15903	1.00E-05	Nil	Australia	MD29	TCTTATTCGTTGGAGACTTTATAACCCT	144.91648
BC15904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85005	WGS84	BC15904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.391	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15904_Cmae_Bot2011_COI	MD27	0	BC15904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15904	Sample	BC15904	targeted	Present	Sample	Cmae_Bot2011_COI	MD27	COI	45	BC15904	1.00E-05	Nil	Australia	MD27	ATGAACAGTCTATCCTCCTTTAG	144.91302
BC15906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84846	WGS84	BC15906	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.604	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15906_Aamu_Bax2006_COI	MD22	0	BC15906_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15906	Sample	BC15906	targeted	Present	Sample	Aamu_Bax2006_COI	MD22	COI	45	BC15906	1.00E-05	Nil	Australia	MD22	GCACAACCGGGATCTTTACTTC	144.90827
BC15906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84846	WGS84	BC15906	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.122	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15906_Sspa_Oph2007_COI	MD22	0	BC15906_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15906	Sample	BC15906	targeted	Present	Sample	Sspa_Oph2007_COI	MD22	COI	45	BC15906	1.00E-05	Nil	Australia	MD22	GAAGGCCTTCCGATGATC	144.90827
BC15906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84846	WGS84	BC15906	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.132	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15906_Upin_Bot2011_COI	MD22	0	BC15906_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15906	Sample	BC15906	targeted	Present	Sample	Upin_Bot2011_COI	MD22	COI	45	BC15906	1.00E-05	Nil	Australia	MD22	CTTTAATTACAGCGTTTTTATTGTTGT	144.90827
BC15907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84561	WGS84	BC15907	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15907_Mgig_Bot2012_COI	MD18	0	BC15907_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15907	Sample	BC15907	targeted	Absent	Sample	Mgig_Bot2012_COI	MD18	COI	45	BC15907	1.00E-05	Nil	Australia	MD18	TCTTATTCGTTGGAGACTTTATAACCCT	144.90755
BC15908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84019	WGS84	BC15908	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15908_Cmae_Bot2011_COI	MD13	0	BC15908_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15908	Sample	BC15908	targeted	Absent	Sample	Cmae_Bot2011_COI	MD13	COI	45	BC15908	1.00E-05	Nil	Australia	MD13	ATGAACAGTCTATCCTCCTTTAG	144.90569
BC15910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82758	WGS84	BC15910	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15910_Mgig_Bot2012_COI	MD126	0	BC15910_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15910	Sample	BC15910	targeted	Absent	Sample	Mgig_Bot2012_COI	MD126	COI	45	BC15910	1.00E-05	Nil	Australia	MD126	TCTTATTCGTTGGAGACTTTATAACCCT	144.89942
BC15911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82593	WGS84	BC15911	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15911_Cmae_Bot2011_COI	MD122	0	BC15911_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15911	Sample	BC15911	targeted	Absent	Sample	Cmae_Bot2011_COI	MD122	COI	45	BC15911	1.00E-05	Nil	Australia	MD122	ATGAACAGTCTATCCTCCTTTAG	144.90066
BC15913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82415	WGS84	BC15913	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.338	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15913_Aamu_Bax2006_COI	MD118	0	BC15913_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15913	Sample	BC15913	targeted	Present	Sample	Aamu_Bax2006_COI	MD118	COI	45	BC15913	1.00E-05	Nil	Australia	MD118	GCACAACCGGGATCTTTACTTC	144.90236
BC15913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82415	WGS84	BC15913	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.794	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15913_Sspa_Oph2007_COI	MD118	0	BC15913_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15913	Sample	BC15913	targeted	Present	Sample	Sspa_Oph2007_COI	MD118	COI	45	BC15913	1.00E-05	Nil	Australia	MD118	GAAGGCCTTCCGATGATC	144.90236
BC15913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82415	WGS84	BC15913	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15913_Upin_Bot2011_COI	MD118	0	BC15913_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15913	Sample	BC15913	targeted	Absent	Sample	Upin_Bot2011_COI	MD118	COI	45	BC15913	1.00E-05	Nil	Australia	MD118	CTTTAATTACAGCGTTTTTATTGTTGT	144.90236
BC15914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8234	WGS84	BC15914	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15914_Mgig_Bot2012_COI	MD116	0	BC15914_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15914	Sample	BC15914	targeted	Absent	Sample	Mgig_Bot2012_COI	MD116	COI	45	BC15914	1.00E-05	Nil	Australia	MD116	TCTTATTCGTTGGAGACTTTATAACCCT	144.906
BC15915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82204	WGS84	BC15915	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15915_Cmae_Bot2011_COI	MD114	0	BC15915_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15915	Sample	BC15915	targeted	Absent	Sample	Cmae_Bot2011_COI	MD114	COI	45	BC15915	1.00E-05	Nil	Australia	MD114	ATGAACAGTCTATCCTCCTTTAG	144.90544
BC15917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82111	WGS84	BC15917	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.945	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15917_Aamu_Bax2006_COI	MD109	0	BC15917_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15917	Sample	BC15917	targeted	Present	Sample	Aamu_Bax2006_COI	MD109	COI	45	BC15917	1.00E-05	Nil	Australia	MD109	GCACAACCGGGATCTTTACTTC	144.90803
BC15917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82111	WGS84	BC15917	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.669	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15917_Sspa_Oph2007_COI	MD109	0	BC15917_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15917	Sample	BC15917	targeted	Present	Sample	Sspa_Oph2007_COI	MD109	COI	45	BC15917	1.00E-05	Nil	Australia	MD109	GAAGGCCTTCCGATGATC	144.90803
BC15917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82111	WGS84	BC15917	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15917_Upin_Bot2011_COI	MD109	0	BC15917_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15917	Sample	BC15917	targeted	Absent	Sample	Upin_Bot2011_COI	MD109	COI	45	BC15917	1.00E-05	Nil	Australia	MD109	CTTTAATTACAGCGTTTTTATTGTTGT	144.90803
BC15918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82047	WGS84	BC15918	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15918_Mgig_Bot2012_COI	MD103	0	BC15918_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15918	Sample	BC15918	targeted	Absent	Sample	Mgig_Bot2012_COI	MD103	COI	45	BC15918	1.00E-05	Nil	Australia	MD103	TCTTATTCGTTGGAGACTTTATAACCCT	144.90914
BC15919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82053	WGS84	BC15919	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.007	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15919_Cmae_Bot2011_COI	MD102	0	BC15919_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15919	Sample	BC15919	targeted	Present	Sample	Cmae_Bot2011_COI	MD102	COI	45	BC15919	1.00E-05	Nil	Australia	MD102	ATGAACAGTCTATCCTCCTTTAG	144.91301
BC15920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.84404	WGS84	BC15920	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.41	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15920_Aamu_Bax2006_COI	MD1	0	BC15920_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15920	Sample	BC15920	targeted	Present	Sample	Aamu_Bax2006_COI	MD1	COI	45	BC15920	1.00E-05	Nil	Australia	MD1	GCACAACCGGGATCTTTACTTC	144.93298
BC15920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.84404	WGS84	BC15920	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.968	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15920_Sspa_Oph2007_COI	MD1	0	BC15920_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15920	Sample	BC15920	targeted	Present	Sample	Sspa_Oph2007_COI	MD1	COI	45	BC15920	1.00E-05	Nil	Australia	MD1	GAAGGCCTTCCGATGATC	144.93298
BC15920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.84404	WGS84	BC15920	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15920_Upin_Bot2011_COI	MD1	0	BC15920_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15920	Sample	BC15920	targeted	Absent	Sample	Upin_Bot2011_COI	MD1	COI	45	BC15920	1.00E-05	Nil	Australia	MD1	CTTTAATTACAGCGTTTTTATTGTTGT	144.93298
BC15921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81914	WGS84	BC15921	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.376	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15921_Mgig_Bot2012_COI	MC97	0	BC15921_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15921	Sample	BC15921	targeted	Present	Sample	Mgig_Bot2012_COI	MC97	COI	45	BC15921	1.00E-05	Nil	Australia	MC97	TCTTATTCGTTGGAGACTTTATAACCCT	144.91473
BC15922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81548	WGS84	BC15922	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15922_Cmae_Bot2011_COI	MC93	0	BC15922_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15922	Sample	BC15922	targeted	Absent	Sample	Cmae_Bot2011_COI	MC93	COI	45	BC15922	1.00E-05	Nil	Australia	MC93	ATGAACAGTCTATCCTCCTTTAG	144.91301
BC15924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81823	WGS84	BC15924	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.4	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15924_Aamu_Bax2006_COI	MC86	0	BC15924_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15924	Sample	BC15924	targeted	Present	Sample	Aamu_Bax2006_COI	MC86	COI	45	BC15924	1.00E-05	Nil	Australia	MC86	GCACAACCGGGATCTTTACTTC	144.91989
BC15924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81823	WGS84	BC15924	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.116	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15924_Sspa_Oph2007_COI	MC86	0	BC15924_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15924	Sample	BC15924	targeted	Present	Sample	Sspa_Oph2007_COI	MC86	COI	45	BC15924	1.00E-05	Nil	Australia	MC86	GAAGGCCTTCCGATGATC	144.91989
BC15924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81823	WGS84	BC15924	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15924_Upin_Bot2011_COI	MC86	0	BC15924_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15924	Sample	BC15924	targeted	Absent	Sample	Upin_Bot2011_COI	MC86	COI	45	BC15924	1.00E-05	Nil	Australia	MC86	CTTTAATTACAGCGTTTTTATTGTTGT	144.91989
BC15925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.818	WGS84	BC15925	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.769	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15925_Mgig_Bot2012_COI	MC84	0	BC15925_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15925	Sample	BC15925	targeted	Present	Sample	Mgig_Bot2012_COI	MC84	COI	45	BC15925	1.00E-05	Nil	Australia	MC84	TCTTATTCGTTGGAGACTTTATAACCCT	144.92319
BC15926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81972	WGS84	BC15926	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15926_Cmae_Bot2011_COI	MC83	0	BC15926_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15926	Sample	BC15926	targeted	Absent	Sample	Cmae_Bot2011_COI	MC83	COI	45	BC15926	1.00E-05	Nil	Australia	MC83	ATGAACAGTCTATCCTCCTTTAG	144.92466
BC15928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81642	WGS84	BC15928	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15928_Aamu_Bax2006_COI	MC79	0	BC15928_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15928	Sample	BC15928	targeted	Absent	Sample	Aamu_Bax2006_COI	MC79	COI	45	BC15928	1.00E-05	Nil	Australia	MC79	GCACAACCGGGATCTTTACTTC	144.92438
BC15928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81642	WGS84	BC15928	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.668	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15928_Sspa_Oph2007_COI	MC79	0	BC15928_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15928	Sample	BC15928	targeted	Present	Sample	Sspa_Oph2007_COI	MC79	COI	45	BC15928	1.00E-05	Nil	Australia	MC79	GAAGGCCTTCCGATGATC	144.92438
BC15928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81642	WGS84	BC15928	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15928_Upin_Bot2011_COI	MC79	0	BC15928_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15928	Sample	BC15928	targeted	Absent	Sample	Upin_Bot2011_COI	MC79	COI	45	BC15928	1.00E-05	Nil	Australia	MC79	CTTTAATTACAGCGTTTTTATTGTTGT	144.92438
BC15929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81951	WGS84	BC15929	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.39	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15929_Mgig_Bot2012_COI	MC76	0	BC15929_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15929	Sample	BC15929	targeted	Present	Sample	Mgig_Bot2012_COI	MC76	COI	45	BC15929	1.00E-05	Nil	Australia	MC76	TCTTATTCGTTGGAGACTTTATAACCCT	144.92794
BC15931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81897	WGS84	BC15931	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15931_Aamu_Bax2006_COI	MC71	0	BC15931_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15931	Sample	BC15931	targeted	Absent	Sample	Aamu_Bax2006_COI	MC71	COI	45	BC15931	1.00E-05	Nil	Australia	MC71	GCACAACCGGGATCTTTACTTC	144.92921
BC15931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81897	WGS84	BC15931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.13	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15931_Sspa_Oph2007_COI	MC71	0	BC15931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15931	Sample	BC15931	targeted	Present	Sample	Sspa_Oph2007_COI	MC71	COI	45	BC15931	1.00E-05	Nil	Australia	MC71	GAAGGCCTTCCGATGATC	144.92921
BC15931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81897	WGS84	BC15931	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15931_Upin_Bot2011_COI	MC71	0	BC15931_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15931	Sample	BC15931	targeted	Absent	Sample	Upin_Bot2011_COI	MC71	COI	45	BC15931	1.00E-05	Nil	Australia	MC71	CTTTAATTACAGCGTTTTTATTGTTGT	144.92921
BC15932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81869	WGS84	BC15932	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.293	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15932_Mgig_Bot2012_COI	MC67	0	BC15932_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15932	Sample	BC15932	targeted	Present	Sample	Mgig_Bot2012_COI	MC67	COI	45	BC15932	1.00E-05	Nil	Australia	MC67	TCTTATTCGTTGGAGACTTTATAACCCT	144.91994
BC15933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81948	WGS84	BC15933	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15933_Cmae_Bot2011_COI	MC63	0	BC15933_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15933	Sample	BC15933	targeted	Absent	Sample	Cmae_Bot2011_COI	MC63	COI	45	BC15933	1.00E-05	Nil	Australia	MC63	ATGAACAGTCTATCCTCCTTTAG	144.91605
BC15935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84165	WGS84	BC15935	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15935_Aamu_Bax2006_COI	MC6	0	BC15935_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15935	Sample	BC15935	targeted	Absent	Sample	Aamu_Bax2006_COI	MC6	COI	45	BC15935	1.00E-05	Nil	Australia	MC6	GCACAACCGGGATCTTTACTTC	144.92945
BC15935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84165	WGS84	BC15935	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.776	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15935_Sspa_Oph2007_COI	MC6	0	BC15935_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15935	Sample	BC15935	targeted	Present	Sample	Sspa_Oph2007_COI	MC6	COI	45	BC15935	1.00E-05	Nil	Australia	MC6	GAAGGCCTTCCGATGATC	144.92945
BC15935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84165	WGS84	BC15935	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15935_Upin_Bot2011_COI	MC6	0	BC15935_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15935	Sample	BC15935	targeted	Absent	Sample	Upin_Bot2011_COI	MC6	COI	45	BC15935	1.00E-05	Nil	Australia	MC6	CTTTAATTACAGCGTTTTTATTGTTGT	144.92945
BC15936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82372	WGS84	BC15936	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.194	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15936_Mgig_Bot2012_COI	MC59	0	BC15936_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15936	Sample	BC15936	targeted	Present	Sample	Mgig_Bot2012_COI	MC59	COI	45	BC15936	1.00E-05	Nil	Australia	MC59	TCTTATTCGTTGGAGACTTTATAACCCT	144.90481
BC15937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82384	WGS84	BC15937	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15937_Cmae_Bot2011_COI	MC58	0	BC15937_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15937	Sample	BC15937	targeted	Absent	Sample	Cmae_Bot2011_COI	MC58	COI	45	BC15937	1.00E-05	Nil	Australia	MC58	ATGAACAGTCTATCCTCCTTTAG	144.90353
BC15939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82484	WGS84	BC15939	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15939_Aamu_Bax2006_COI	MC56	0	BC15939_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15939	Sample	BC15939	targeted	Absent	Sample	Aamu_Bax2006_COI	MC56	COI	45	BC15939	1.00E-05	Nil	Australia	MC56	GCACAACCGGGATCTTTACTTC	144.90287
BC15939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82484	WGS84	BC15939	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.742	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15939_Sspa_Oph2007_COI	MC56	0	BC15939_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15939	Sample	BC15939	targeted	Present	Sample	Sspa_Oph2007_COI	MC56	COI	45	BC15939	1.00E-05	Nil	Australia	MC56	GAAGGCCTTCCGATGATC	144.90287
BC15939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82484	WGS84	BC15939	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15939_Upin_Bot2011_COI	MC56	0	BC15939_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15939	Sample	BC15939	targeted	Absent	Sample	Upin_Bot2011_COI	MC56	COI	45	BC15939	1.00E-05	Nil	Australia	MC56	CTTTAATTACAGCGTTTTTATTGTTGT	144.90287
BC15940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82722	WGS84	BC15940	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15940_Cmae_Bot2011_COI	MC51	0	BC15940_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15940	Sample	BC15940	targeted	Absent	Sample	Cmae_Bot2011_COI	MC51	COI	45	BC15940	1.00E-05	Nil	Australia	MC51	ATGAACAGTCTATCCTCCTTTAG	144.90064
BC15942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8663	WGS84	BC15942	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15942_Aamu_Bax2006_COI	MC31	0	BC15942_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15942	Sample	BC15942	targeted	Absent	Sample	Aamu_Bax2006_COI	MC31	COI	45	BC15942	1.00E-05	Nil	Australia	MC31	GCACAACCGGGATCTTTACTTC	144.91593
BC15942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8663	WGS84	BC15942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15942_Sspa_Oph2007_COI	MC31	0	BC15942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15942	Sample	BC15942	targeted	Absent	Sample	Sspa_Oph2007_COI	MC31	COI	45	BC15942	1.00E-05	Nil	Australia	MC31	GAAGGCCTTCCGATGATC	144.91593
BC15942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8663	WGS84	BC15942	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.226	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15942_Upin_Bot2011_COI	MC31	0	BC15942_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15942	Sample	BC15942	targeted	Present	Sample	Upin_Bot2011_COI	MC31	COI	45	BC15942	1.00E-05	Nil	Australia	MC31	CTTTAATTACAGCGTTTTTATTGTTGT	144.91593
BC15943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8442	WGS84	BC15943	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.905	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15943_Mgig_Bot2012_COI	MC3	0	BC15943_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15943	Sample	BC15943	targeted	Present	Sample	Mgig_Bot2012_COI	MC3	COI	45	BC15943	1.00E-05	Nil	Australia	MC3	TCTTATTCGTTGGAGACTTTATAACCCT	144.92999
BC15944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84007	WGS84	BC15944	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15944_Cmae_Bot2011_COI	MC28	0	BC15944_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15944	Sample	BC15944	targeted	Absent	Sample	Cmae_Bot2011_COI	MC28	COI	45	BC15944	1.00E-05	Nil	Australia	MC28	ATGAACAGTCTATCCTCCTTTAG	144.90559
BC15946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84441	WGS84	BC15946	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.814	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15946_Aamu_Bax2006_COI	MC23	0	BC15946_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15946	Sample	BC15946	targeted	Present	Sample	Aamu_Bax2006_COI	MC23	COI	45	BC15946	1.00E-05	Nil	Australia	MC23	GCACAACCGGGATCTTTACTTC	144.90733
BC15946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84441	WGS84	BC15946	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.326	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15946_Sspa_Oph2007_COI	MC23	0	BC15946_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15946	Sample	BC15946	targeted	Present	Sample	Sspa_Oph2007_COI	MC23	COI	45	BC15946	1.00E-05	Nil	Australia	MC23	GAAGGCCTTCCGATGATC	144.90733
BC15946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84441	WGS84	BC15946	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15946_Upin_Bot2011_COI	MC23	0	BC15946_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15946	Sample	BC15946	targeted	Absent	Sample	Upin_Bot2011_COI	MC23	COI	45	BC15946	1.00E-05	Nil	Australia	MC23	CTTTAATTACAGCGTTTTTATTGTTGT	144.90733
BC15947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84617	WGS84	BC15947	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.234	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15947_Mgig_Bot2012_COI	MC21	0	BC15947_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15947	Sample	BC15947	targeted	Present	Sample	Mgig_Bot2012_COI	MC21	COI	45	BC15947	1.00E-05	Nil	Australia	MC21	TCTTATTCGTTGGAGACTTTATAACCCT	144.90876
BC15948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84791	WGS84	BC15948	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15948_Cmae_Bot2011_COI	MC19	0	BC15948_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15948	Sample	BC15948	targeted	Absent	Sample	Cmae_Bot2011_COI	MC19	COI	45	BC15948	1.00E-05	Nil	Australia	MC19	ATGAACAGTCTATCCTCCTTTAG	144.90758
BC15950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8496	WGS84	BC15950	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.209	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15950_Mgig_Bot2012_COI	MC14	0	BC15950_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15950	Sample	BC15950	targeted	Present	Sample	Mgig_Bot2012_COI	MC14	COI	45	BC15950	1.00E-05	Nil	Australia	MC14	TCTTATTCGTTGGAGACTTTATAACCCT	144.90892
BC15951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85836	WGS84	BC15951	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15951_Cmae_Bot2011_COI	MC122	0	BC15951_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15951	Sample	BC15951	targeted	Absent	Sample	Cmae_Bot2011_COI	MC122	COI	45	BC15951	1.00E-05	Nil	Australia	MC122	ATGAACAGTCTATCCTCCTTTAG	144.90732
BC15953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85827	WGS84	BC15953	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15953_Aamu_Bax2006_COI	MC121	0	BC15953_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15953	Sample	BC15953	targeted	Absent	Sample	Aamu_Bax2006_COI	MC121	COI	45	BC15953	1.00E-05	Nil	Australia	MC121	GCACAACCGGGATCTTTACTTC	144.90522
BC15953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85827	WGS84	BC15953	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.386	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15953_Sspa_Oph2007_COI	MC121	0	BC15953_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15953	Sample	BC15953	targeted	Present	Sample	Sspa_Oph2007_COI	MC121	COI	45	BC15953	1.00E-05	Nil	Australia	MC121	GAAGGCCTTCCGATGATC	144.90522
BC15953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85827	WGS84	BC15953	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15953_Upin_Bot2011_COI	MC121	0	BC15953_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15953	Sample	BC15953	targeted	Absent	Sample	Upin_Bot2011_COI	MC121	COI	45	BC15953	1.00E-05	Nil	Australia	MC121	CTTTAATTACAGCGTTTTTATTGTTGT	144.90522
BC15954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82137	WGS84	BC15954	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.127	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15954_Mgig_Bot2012_COI	MC113	0	BC15954_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15954	Sample	BC15954	targeted	Present	Sample	Mgig_Bot2012_COI	MC113	COI	45	BC15954	1.00E-05	Nil	Australia	MC113	TCTTATTCGTTGGAGACTTTATAACCCT	144.91095
BC15955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82293	WGS84	BC15955	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15955_Cmae_Bot2011_COI	MC112	0	BC15955_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15955	Sample	BC15955	targeted	Absent	Sample	Cmae_Bot2011_COI	MC112	COI	45	BC15955	1.00E-05	Nil	Australia	MC112	ATGAACAGTCTATCCTCCTTTAG	144.90727
BC15957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84766	WGS84	BC15957	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.519	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15957_Aamu_Bax2006_COI	MC11	0	BC15957_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15957	Sample	BC15957	targeted	Present	Sample	Aamu_Bax2006_COI	MC11	COI	45	BC15957	1.00E-05	Nil	Australia	MC11	GCACAACCGGGATCTTTACTTC	144.93078
BC15957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84766	WGS84	BC15957	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.304	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15957_Sspa_Oph2007_COI	MC11	0	BC15957_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15957	Sample	BC15957	targeted	Present	Sample	Sspa_Oph2007_COI	MC11	COI	45	BC15957	1.00E-05	Nil	Australia	MC11	GAAGGCCTTCCGATGATC	144.93078
BC15957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84766	WGS84	BC15957	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15957_Upin_Bot2011_COI	MC11	0	BC15957_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15957	Sample	BC15957	targeted	Absent	Sample	Upin_Bot2011_COI	MC11	COI	45	BC15957	1.00E-05	Nil	Australia	MC11	CTTTAATTACAGCGTTTTTATTGTTGT	144.93078
BC15958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81946	WGS84	BC15958	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.632	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15958_Mgig_Bot2012_COI	MC109	0	BC15958_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15958	Sample	BC15958	targeted	Present	Sample	Mgig_Bot2012_COI	MC109	COI	45	BC15958	1.00E-05	Nil	Australia	MC109	TCTTATTCGTTGGAGACTTTATAACCCT	144.91081
BC15959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81921	WGS84	BC15959	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15959_Cmae_Bot2011_COI	MC103	0	BC15959_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15959	Sample	BC15959	targeted	Absent	Sample	Cmae_Bot2011_COI	MC103	COI	45	BC15959	1.00E-05	Nil	Australia	MC103	ATGAACAGTCTATCCTCCTTTAG	144.92594
BC15960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81039	WGS84	BC15960	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15960_Aamu_Bax2006_COI	MB99	0	BC15960_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15960	Sample	BC15960	targeted	Absent	Sample	Aamu_Bax2006_COI	MB99	COI	45	BC15960	1.00E-05	Nil	Australia	MB99	GCACAACCGGGATCTTTACTTC	144.91343
BC15960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81039	WGS84	BC15960	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.076	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15960_Sspa_Oph2007_COI	MB99	0	BC15960_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15960	Sample	BC15960	targeted	Present	Sample	Sspa_Oph2007_COI	MB99	COI	45	BC15960	1.00E-05	Nil	Australia	MB99	GAAGGCCTTCCGATGATC	144.91343
BC15960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81039	WGS84	BC15960	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15960_Upin_Bot2011_COI	MB99	0	BC15960_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15960	Sample	BC15960	targeted	Absent	Sample	Upin_Bot2011_COI	MB99	COI	45	BC15960	1.00E-05	Nil	Australia	MB99	CTTTAATTACAGCGTTTTTATTGTTGT	144.91343
BC15961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.80951	WGS84	BC15961	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.118	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15961_Mgig_Bot2012_COI	MB97	0	BC15961_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15961	Sample	BC15961	targeted	Present	Sample	Mgig_Bot2012_COI	MB97	COI	45	BC15961	1.00E-05	Nil	Australia	MB97	TCTTATTCGTTGGAGACTTTATAACCCT	144.91335
BC15962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81351	WGS84	BC15962	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15962_Cmae_Bot2011_COI	MB93	0	BC15962_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15962	Sample	BC15962	targeted	Absent	Sample	Cmae_Bot2011_COI	MB93	COI	45	BC15962	1.00E-05	Nil	Australia	MB93	ATGAACAGTCTATCCTCCTTTAG	144.91416
BC15964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81861	WGS84	BC15964	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15964_Aamu_Bax2006_COI	MB88	0	BC15964_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15964	Sample	BC15964	targeted	Absent	Sample	Aamu_Bax2006_COI	MB88	COI	45	BC15964	1.00E-05	Nil	Australia	MB88	GCACAACCGGGATCTTTACTTC	144.9163
BC15964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81861	WGS84	BC15964	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.793	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15964_Sspa_Oph2007_COI	MB88	0	BC15964_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15964	Sample	BC15964	targeted	Present	Sample	Sspa_Oph2007_COI	MB88	COI	45	BC15964	1.00E-05	Nil	Australia	MB88	GAAGGCCTTCCGATGATC	144.9163
BC15964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81861	WGS84	BC15964	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15964_Upin_Bot2011_COI	MB88	0	BC15964_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15964	Sample	BC15964	targeted	Absent	Sample	Upin_Bot2011_COI	MB88	COI	45	BC15964	1.00E-05	Nil	Australia	MB88	CTTTAATTACAGCGTTTTTATTGTTGT	144.9163
BC15965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81788	WGS84	BC15965	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.43	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15965_Mgig_Bot2012_COI	MB86	0	BC15965_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15965	Sample	BC15965	targeted	Present	Sample	Mgig_Bot2012_COI	MB86	COI	45	BC15965	1.00E-05	Nil	Australia	MB86	TCTTATTCGTTGGAGACTTTATAACCCT	144.91938
BC15966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81667	WGS84	BC15966	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15966_Cmae_Bot2011_COI	MB84	0	BC15966_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15966	Sample	BC15966	targeted	Absent	Sample	Cmae_Bot2011_COI	MB84	COI	45	BC15966	1.00E-05	Nil	Australia	MB84	ATGAACAGTCTATCCTCCTTTAG	144.92252
BC15968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84347	WGS84	BC15968	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.117	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15968_Aamu_Bax2006_COI	MB8	0	BC15968_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15968	Sample	BC15968	targeted	Present	Sample	Aamu_Bax2006_COI	MB8	COI	45	BC15968	1.00E-05	Nil	Australia	MB8	GCACAACCGGGATCTTTACTTC	144.90609
BC15968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84347	WGS84	BC15968	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.024	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15968_Sspa_Oph2007_COI	MB8	0	BC15968_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15968	Sample	BC15968	targeted	Present	Sample	Sspa_Oph2007_COI	MB8	COI	45	BC15968	1.00E-05	Nil	Australia	MB8	GAAGGCCTTCCGATGATC	144.90609
BC15968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84347	WGS84	BC15968	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.398	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15968_Upin_Bot2011_COI	MB8	0	BC15968_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15968	Sample	BC15968	targeted	Present	Sample	Upin_Bot2011_COI	MB8	COI	45	BC15968	1.00E-05	Nil	Australia	MB8	CTTTAATTACAGCGTTTTTATTGTTGT	144.90609
BC15969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81593	WGS84	BC15969	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15969_Mgig_Bot2012_COI	MB79	0	BC15969_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15969	Sample	BC15969	targeted	Absent	Sample	Mgig_Bot2012_COI	MB79	COI	45	BC15969	1.00E-05	Nil	Australia	MB79	TCTTATTCGTTGGAGACTTTATAACCCT	144.92751
BC15971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8205	WGS84	BC15971	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15971_Aamu_Bax2006_COI	MB68	0	BC15971_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15971	Sample	BC15971	targeted	Absent	Sample	Aamu_Bax2006_COI	MB68	COI	45	BC15971	1.00E-05	Nil	Australia	MB68	GCACAACCGGGATCTTTACTTC	144.92968
BC15971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8205	WGS84	BC15971	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15971_Sspa_Oph2007_COI	MB68	0	BC15971_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15971	Sample	BC15971	targeted	Absent	Sample	Sspa_Oph2007_COI	MB68	COI	45	BC15971	1.00E-05	Nil	Australia	MB68	GAAGGCCTTCCGATGATC	144.92968
BC15971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8205	WGS84	BC15971	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15971_Upin_Bot2011_COI	MB68	0	BC15971_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15971	Sample	BC15971	targeted	Absent	Sample	Upin_Bot2011_COI	MB68	COI	45	BC15971	1.00E-05	Nil	Australia	MB68	CTTTAATTACAGCGTTTTTATTGTTGT	144.92968
BC15972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81981	WGS84	BC15972	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.272	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15972_Mgig_Bot2012_COI	MB64	0	BC15972_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15972	Sample	BC15972	targeted	Present	Sample	Mgig_Bot2012_COI	MB64	COI	45	BC15972	1.00E-05	Nil	Australia	MB64	TCTTATTCGTTGGAGACTTTATAACCCT	144.92026
BC15973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84301	WGS84	BC15973	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15973_Cmae_Bot2011_COI	MB6	0	BC15973_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15973	Sample	BC15973	targeted	Absent	Sample	Cmae_Bot2011_COI	MB6	COI	45	BC15973	1.00E-05	Nil	Australia	MB6	ATGAACAGTCTATCCTCCTTTAG	144.90526
BC15975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82651	WGS84	BC15975	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.468	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15975_Aamu_Bax2006_COI	MB57	0	BC15975_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15975	Sample	BC15975	targeted	Present	Sample	Aamu_Bax2006_COI	MB57	COI	45	BC15975	1.00E-05	Nil	Australia	MB57	GCACAACCGGGATCTTTACTTC	144.90242
BC15975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82651	WGS84	BC15975	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.961	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15975_Sspa_Oph2007_COI	MB57	0	BC15975_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15975	Sample	BC15975	targeted	Present	Sample	Sspa_Oph2007_COI	MB57	COI	45	BC15975	1.00E-05	Nil	Australia	MB57	GAAGGCCTTCCGATGATC	144.90242
BC15975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82651	WGS84	BC15975	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.349	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15975_Upin_Bot2011_COI	MB57	0	BC15975_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15975	Sample	BC15975	targeted	Present	Sample	Upin_Bot2011_COI	MB57	COI	45	BC15975	1.00E-05	Nil	Australia	MB57	CTTTAATTACAGCGTTTTTATTGTTGT	144.90242
BC15976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85669	WGS84	BC15976	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15976_Mgig_Bot2012_COI	MB53	0	BC15976_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15976	Sample	BC15976	targeted	Absent	Sample	Mgig_Bot2012_COI	MB53	COI	45	BC15976	1.00E-05	Nil	Australia	MB53	TCTTATTCGTTGGAGACTTTATAACCCT	144.90554
BC15977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85954	WGS84	BC15977	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15977_Cmae_Bot2011_COI	MB51	0	BC15977_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15977	Sample	BC15977	targeted	Absent	Sample	Cmae_Bot2011_COI	MB51	COI	45	BC15977	1.00E-05	Nil	Australia	MB51	ATGAACAGTCTATCCTCCTTTAG	144.90766
BC15979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86151	WGS84	BC15979	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.507	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15979_Aamu_Bax2006_COI	MB44	0	BC15979_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15979	Sample	BC15979	targeted	Present	Sample	Aamu_Bax2006_COI	MB44	COI	45	BC15979	1.00E-05	Nil	Australia	MB44	GCACAACCGGGATCTTTACTTC	144.90993
BC15979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86151	WGS84	BC15979	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.431	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15979_Sspa_Oph2007_COI	MB44	0	BC15979_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15979	Sample	BC15979	targeted	Present	Sample	Sspa_Oph2007_COI	MB44	COI	45	BC15979	1.00E-05	Nil	Australia	MB44	GAAGGCCTTCCGATGATC	144.90993
BC15979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86151	WGS84	BC15979	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.638	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15979_Upin_Bot2011_COI	MB44	0	BC15979_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15979	Sample	BC15979	targeted	Present	Sample	Upin_Bot2011_COI	MB44	COI	45	BC15979	1.00E-05	Nil	Australia	MB44	CTTTAATTACAGCGTTTTTATTGTTGT	144.90993
BC15980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86182	WGS84	BC15980	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15980_Cmae_Bot2011_COI	MB42	0	BC15980_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15980	Sample	BC15980	targeted	Absent	Sample	Cmae_Bot2011_COI	MB42	COI	45	BC15980	1.00E-05	Nil	Australia	MB42	ATGAACAGTCTATCCTCCTTTAG	144.91412
BC15982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84074	WGS84	BC15982	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.496	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15982_Aamu_Bax2006_COI	MB4	0	BC15982_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15982	Sample	BC15982	targeted	Present	Sample	Aamu_Bax2006_COI	MB4	COI	45	BC15982	1.00E-05	Nil	Australia	MB4	GCACAACCGGGATCTTTACTTC	144.90425
BC15982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84074	WGS84	BC15982	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.009	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15982_Sspa_Oph2007_COI	MB4	0	BC15982_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15982	Sample	BC15982	targeted	Present	Sample	Sspa_Oph2007_COI	MB4	COI	45	BC15982	1.00E-05	Nil	Australia	MB4	GAAGGCCTTCCGATGATC	144.90425
BC15982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84074	WGS84	BC15982	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.316	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15982_Upin_Bot2011_COI	MB4	0	BC15982_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15982	Sample	BC15982	targeted	Present	Sample	Upin_Bot2011_COI	MB4	COI	45	BC15982	1.00E-05	Nil	Australia	MB4	CTTTAATTACAGCGTTTTTATTGTTGT	144.90425
BC15983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8632	WGS84	BC15983	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15983_Mgig_Bot2012_COI	MB37	0	BC15983_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15983	Sample	BC15983	targeted	Absent	Sample	Mgig_Bot2012_COI	MB37	COI	45	BC15983	1.00E-05	Nil	Australia	MB37	TCTTATTCGTTGGAGACTTTATAACCCT	144.91774
BC15984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86568	WGS84	BC15984	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15984_Cmae_Bot2011_COI	MB36	0	BC15984_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15984	Sample	BC15984	targeted	Absent	Sample	Cmae_Bot2011_COI	MB36	COI	45	BC15984	1.00E-05	Nil	Australia	MB36	ATGAACAGTCTATCCTCCTTTAG	144.91795
BC15986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86421	WGS84	BC15986	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.626	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15986_Aamu_Bax2006_COI	MB31	0	BC15986_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15986	Sample	BC15986	targeted	Present	Sample	Aamu_Bax2006_COI	MB31	COI	45	BC15986	1.00E-05	Nil	Australia	MB31	GCACAACCGGGATCTTTACTTC	144.91498
BC15986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86421	WGS84	BC15986	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.299	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15986_Sspa_Oph2007_COI	MB31	0	BC15986_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15986	Sample	BC15986	targeted	Present	Sample	Sspa_Oph2007_COI	MB31	COI	45	BC15986	1.00E-05	Nil	Australia	MB31	GAAGGCCTTCCGATGATC	144.91498
BC15986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86421	WGS84	BC15986	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.17	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15986_Upin_Bot2011_COI	MB31	0	BC15986_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15986	Sample	BC15986	targeted	Present	Sample	Upin_Bot2011_COI	MB31	COI	45	BC15986	1.00E-05	Nil	Australia	MB31	CTTTAATTACAGCGTTTTTATTGTTGT	144.91498
BC15987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84065	WGS84	BC15987	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15987_Mgig_Bot2012_COI	MB3	0	BC15987_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15987	Sample	BC15987	targeted	Absent	Sample	Mgig_Bot2012_COI	MB3	COI	45	BC15987	1.00E-05	Nil	Australia	MB3	TCTTATTCGTTGGAGACTTTATAACCCT	144.90472
BC15988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86288	WGS84	BC15988	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15988_Cmae_Bot2011_COI	MB29	0	BC15988_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15988	Sample	BC15988	targeted	Absent	Sample	Cmae_Bot2011_COI	MB29	COI	45	BC15988	1.00E-05	Nil	Australia	MB29	ATGAACAGTCTATCCTCCTTTAG	144.91667
BC25831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80376	WGS84	BC25831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25831_Aamu_Bax2006_COI	T18-05	1	BC25831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25831	Sample	BC25831	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-05	COI	45	BC25831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-05	GCACAACCGGGATCTTTACTTC	147.52542
BC25831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80376	WGS84	BC25831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25831_Pcan_Bot2011_IGS	T18-05	1	BC25831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25831	Sample	BC25831	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-05	IGS	45	BC25831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-05	CGTAATCCTCAGTACTGGCTA	147.52542
BC25831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80376	WGS84	BC25831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25831_Sspa_Oph2007_COI	T18-05	1	BC25831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25831	Sample	BC25831	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-05	COI	45	BC25831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-05	GAAGGCCTTCCGATGATC	147.52542
BC25831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80376	WGS84	BC25831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25831_Upin_Bot2011_COI	T18-05	1	BC25831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25831	Sample	BC25831	targeted	Absent	Sample	Upin_Bot2011_COI	T18-05	COI	45	BC25831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.52542
BC25832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25832	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25832_Cjap_Sim2018_COI	T18-06	1	BC25832_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25832	Sample	BC25832	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-06	COI	45	BC25832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.48547
BC25832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.082	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	28/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25832_Mgig_Bot2012_COI	T18-06	1	BC25832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25832	Sample	BC25832	targeted	Present	Sample	Mgig_Bot2012_COI	T18-06	COI	45	BC25832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.48547
BC25833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80019	WGS84	BC25833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.232	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25833_Cmae_Bot2011_COI	T18-07	1	BC25833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25833	Sample	BC25833	targeted	Present	Sample	Cmae_Bot2011_COI	T18-07	COI	45	BC25833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-07	ATGAACAGTCTATCCTCCTTTAG	147.46497
BC25835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96402	WGS84	BC25835	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25835_Aamu_Bax2006_COI	T18-12	1	BC25835_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25835	Sample	BC25835	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-12	COI	45	BC25835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-12	GCACAACCGGGATCTTTACTTC	147.53822
BC25835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96402	WGS84	BC25835	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25835_Pcan_Bot2011_IGS	T18-12	1	BC25835_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25835	Sample	BC25835	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-12	IGS	45	BC25835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-12	CGTAATCCTCAGTACTGGCTA	147.53822
BC25835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96402	WGS84	BC25835	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25835_Sspa_Oph2007_COI	T18-12	1	BC25835_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25835	Sample	BC25835	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-12	COI	45	BC25835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-12	GAAGGCCTTCCGATGATC	147.53822
BC25835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96402	WGS84	BC25835	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25835_Upin_Bot2011_COI	T18-12	1	BC25835_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25835	Sample	BC25835	targeted	Absent	Sample	Upin_Bot2011_COI	T18-12	COI	45	BC25835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.53822
BC25836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95973	WGS84	BC25836	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25836_Cjap_Sim2018_COI	T18-13	1	BC25836_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25836	Sample	BC25836	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-13	COI	45	BC25836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52782
BC25836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95973	WGS84	BC25836	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.267	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	14/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25836_Mgig_Bot2012_COI	T18-13	1	BC25836_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25836	Sample	BC25836	targeted	Present	Sample	Mgig_Bot2012_COI	T18-13	COI	45	BC25836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.52782
BC25837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25837	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.076	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25837_Cmae_Bot2011_COI	T18-14	1	BC25837_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25837	Sample	BC25837	targeted	Present	Sample	Cmae_Bot2011_COI	T18-14	COI	45	BC25837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-14	ATGAACAGTCTATCCTCCTTTAG	147.52367
BC25840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25840	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.199	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25840_Cmae_Bot2011_COI	T18-17	1	BC25840_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25840	Sample	BC25840	targeted	Present	Sample	Cmae_Bot2011_COI	T18-17	COI	45	BC25840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-17	ATGAACAGTCTATCCTCCTTTAG	147.48547
BC25842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84602	WGS84	BC25842	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25842_Aamu_Bax2006_COI	S13-02	1	BC25842_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25842	Sample	BC25842	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-02	COI	45	BC25842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-02	GCACAACCGGGATCTTTACTTC	138.4969
BC25842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84602	WGS84	BC25842	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25842_Pcan_Bot2011_IGS	S13-02	1	BC25842_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25842	Sample	BC25842	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-02	IGS	45	BC25842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-02	CGTAATCCTCAGTACTGGCTA	138.4969
BC25842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84602	WGS84	BC25842	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.558	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25842_Sspa_Oph2007_COI	S13-02	1	BC25842_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25842	Sample	BC25842	targeted	Present	Sample	Sspa_Oph2007_COI	S13-02	COI	45	BC25842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-02	GAAGGCCTTCCGATGATC	138.4969
BC25842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84602	WGS84	BC25842	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25842_Upin_Bot2011_COI	S13-02	1	BC25842_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25842	Sample	BC25842	targeted	Absent	Sample	Upin_Bot2011_COI	S13-02	COI	45	BC25842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.4969
BC25843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84521	WGS84	BC25843	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25843_Cjap_Sim2018_COI	S13-03	1	BC25843_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25843	Sample	BC25843	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-03	COI	45	BC25843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4969
BC25843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84521	WGS84	BC25843	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.656	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25843_Mgig_Bot2012_COI	S13-03	1	BC25843_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25843	Sample	BC25843	targeted	Present	Sample	Mgig_Bot2012_COI	S13-03	COI	45	BC25843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.4969
BC25844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25844	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25844_Cmae_Bot2011_COI	S13-04	1	BC25844_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25844	Sample	BC25844	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-04	COI	45	BC25844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-04	ATGAACAGTCTATCCTCCTTTAG	138.50784
BC25846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83708	WGS84	BC25846	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25846_Aamu_Bax2006_COI	S13-06	1	BC25846_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25846	Sample	BC25846	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-06	COI	45	BC25846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-06	GCACAACCGGGATCTTTACTTC	138.51062
BC25846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83708	WGS84	BC25846	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25846_Pcan_Bot2011_IGS	S13-06	1	BC25846_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25846	Sample	BC25846	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-06	IGS	45	BC25846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-06	CGTAATCCTCAGTACTGGCTA	138.51062
BC25846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83708	WGS84	BC25846	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.119	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25846_Sspa_Oph2007_COI	S13-06	1	BC25846_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25846	Sample	BC25846	targeted	Present	Sample	Sspa_Oph2007_COI	S13-06	COI	45	BC25846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-06	GAAGGCCTTCCGATGATC	138.51062
BC25846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83708	WGS84	BC25846	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25846_Upin_Bot2011_COI	S13-06	1	BC25846_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25846	Sample	BC25846	targeted	Absent	Sample	Upin_Bot2011_COI	S13-06	COI	45	BC25846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.51062
BC25847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.7778	WGS84	BC25847	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25847_Cjap_Sim2018_COI	S13-07	1	BC25847_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25847	Sample	BC25847	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-07	COI	45	BC25847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51836
BC25847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.7778	WGS84	BC25847	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.241	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25847_Mgig_Bot2012_COI	S13-07	1	BC25847_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25847	Sample	BC25847	targeted	Present	Sample	Mgig_Bot2012_COI	S13-07	COI	45	BC25847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51836
BC25848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77898	WGS84	BC25848	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25848_Cmae_Bot2011_COI	S13-08	1	BC25848_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25848	Sample	BC25848	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-08	COI	45	BC25848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-08	ATGAACAGTCTATCCTCCTTTAG	138.51781
BC25850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80929	WGS84	BC25850	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25850_Cjap_Sim2018_COI	S13-10	1	BC25850_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25850	Sample	BC25850	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-10	COI	45	BC25850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51861
BC25850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80929	WGS84	BC25850	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.022	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25850_Mgig_Bot2012_COI	S13-10	1	BC25850_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25850	Sample	BC25850	targeted	Present	Sample	Mgig_Bot2012_COI	S13-10	COI	45	BC25850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.51861
BC25851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80998	WGS84	BC25851	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25851_Cmae_Bot2011_COI	S13-11	1	BC25851_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25851	Sample	BC25851	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-11	COI	45	BC25851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-11	ATGAACAGTCTATCCTCCTTTAG	138.51973
BC25853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83975	WGS84	BC25853	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25853_Aamu_Bax2006_COI	S14-01	1	BC25853_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25853	Sample	BC25853	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-01	COI	45	BC25853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-01	GCACAACCGGGATCTTTACTTC	138.50678
BC25853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83975	WGS84	BC25853	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25853_Pcan_Bot2011_IGS	S14-01	1	BC25853_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25853	Sample	BC25853	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-01	IGS	45	BC25853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-01	CGTAATCCTCAGTACTGGCTA	138.50678
BC25853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83975	WGS84	BC25853	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25853_Sspa_Oph2007_COI	S14-01	1	BC25853_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25853	Sample	BC25853	targeted	Absent	Sample	Sspa_Oph2007_COI	S14-01	COI	45	BC25853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-01	GAAGGCCTTCCGATGATC	138.50678
BC25853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83975	WGS84	BC25853	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25853_Upin_Bot2011_COI	S14-01	1	BC25853_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25853	Sample	BC25853	targeted	Absent	Sample	Upin_Bot2011_COI	S14-01	COI	45	BC25853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.50678
BC25854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25854	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25854_Cjap_Sim2018_COI	S14-02	1	BC25854_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25854	Sample	BC25854	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-02	COI	45	BC25854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50776
BC25854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25854	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.093	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25854_Mgig_Bot2012_COI	S14-02	1	BC25854_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25854	Sample	BC25854	targeted	Present	Sample	Mgig_Bot2012_COI	S14-02	COI	45	BC25854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.50776
BC25855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.8365	WGS84	BC25855	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25855_Cmae_Bot2011_COI	S14-03	1	BC25855_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25855	Sample	BC25855	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-03	COI	45	BC25855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-03	ATGAACAGTCTATCCTCCTTTAG	138.50906
BC25857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83382	WGS84	BC25857	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25857_Aamu_Bax2006_COI	S14-05	1	BC25857_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25857	Sample	BC25857	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-05	COI	45	BC25857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-05	GCACAACCGGGATCTTTACTTC	138.50779
BC25857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83382	WGS84	BC25857	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25857_Pcan_Bot2011_IGS	S14-05	1	BC25857_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25857	Sample	BC25857	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-05	IGS	45	BC25857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-05	CGTAATCCTCAGTACTGGCTA	138.50779
BC25857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83382	WGS84	BC25857	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25857_Sspa_Oph2007_COI	S14-05	1	BC25857_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25857	Sample	BC25857	targeted	Absent	Sample	Sspa_Oph2007_COI	S14-05	COI	45	BC25857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-05	GAAGGCCTTCCGATGATC	138.50779
BC25857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83382	WGS84	BC25857	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25857_Upin_Bot2011_COI	S14-05	1	BC25857_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25857	Sample	BC25857	targeted	Absent	Sample	Upin_Bot2011_COI	S14-05	COI	45	BC25857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.50779
BC25858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.809	WGS84	BC25858	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25858_Cjap_Sim2018_COI	S14-06	1	BC25858_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25858	Sample	BC25858	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-06	COI	45	BC25858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51736
BC25858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.809	WGS84	BC25858	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.481	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25858_Mgig_Bot2012_COI	S14-06	1	BC25858_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25858	Sample	BC25858	targeted	Present	Sample	Mgig_Bot2012_COI	S14-06	COI	45	BC25858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.51736
BC25859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80934	WGS84	BC25859	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25859_Cmae_Bot2011_COI	S14-07	1	BC25859_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25859	Sample	BC25859	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-07	COI	45	BC25859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-07	ATGAACAGTCTATCCTCCTTTAG	138.51813
BC25860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80988	WGS84	BC25860	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25860_Aamu_Bax2006_COI	S14-08	1	BC25860_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25860	Sample	BC25860	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-08	COI	45	BC25860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-08	GCACAACCGGGATCTTTACTTC	138.51941
BC25860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80988	WGS84	BC25860	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25860_Pcan_Bot2011_IGS	S14-08	1	BC25860_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25860	Sample	BC25860	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-08	IGS	45	BC25860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-08	CGTAATCCTCAGTACTGGCTA	138.51941
BC25860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80988	WGS84	BC25860	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.438	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25860_Sspa_Oph2007_COI	S14-08	1	BC25860_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25860	Sample	BC25860	targeted	Present	Sample	Sspa_Oph2007_COI	S14-08	COI	45	BC25860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-08	GAAGGCCTTCCGATGATC	138.51941
BC25860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80988	WGS84	BC25860	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25860_Upin_Bot2011_COI	S14-08	1	BC25860_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25860	Sample	BC25860	targeted	Absent	Sample	Upin_Bot2011_COI	S14-08	COI	45	BC25860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51941
BC25861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81024	WGS84	BC25861	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25861_Cjap_Sim2018_COI	S14-09	1	BC25861_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25861	Sample	BC25861	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-09	COI	45	BC25861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52043
BC25861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81024	WGS84	BC25861	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.907	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25861_Mgig_Bot2012_COI	S14-09	1	BC25861_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25861	Sample	BC25861	targeted	Present	Sample	Mgig_Bot2012_COI	S14-09	COI	45	BC25861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.52043
BC25862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81126	WGS84	BC25862	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25862_Cmae_Bot2011_COI	S14-10	1	BC25862_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25862	Sample	BC25862	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-10	COI	45	BC25862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-10	ATGAACAGTCTATCCTCCTTTAG	138.52292
BC25864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77271	WGS84	BC25864	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25864_Aamu_Bax2006_COI	S15-02	1	BC25864_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25864	Sample	BC25864	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-02	COI	45	BC25864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-02	GCACAACCGGGATCTTTACTTC	138.48516
BC25864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77271	WGS84	BC25864	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25864_Pcan_Bot2011_IGS	S15-02	1	BC25864_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25864	Sample	BC25864	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-02	IGS	45	BC25864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-02	CGTAATCCTCAGTACTGGCTA	138.48516
BC25864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77271	WGS84	BC25864	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25864_Sspa_Oph2007_COI	S15-02	1	BC25864_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25864	Sample	BC25864	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-02	COI	45	BC25864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-02	GAAGGCCTTCCGATGATC	138.48516
BC25864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77271	WGS84	BC25864	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25864_Upin_Bot2011_COI	S15-02	1	BC25864_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25864	Sample	BC25864	targeted	Absent	Sample	Upin_Bot2011_COI	S15-02	COI	45	BC25864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.48516
BC25865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77213	WGS84	BC25865	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25865_Cjap_Sim2018_COI	S15-03	1	BC25865_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25865	Sample	BC25865	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-03	COI	45	BC25865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48451
BC25865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77213	WGS84	BC25865	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.749	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25865_Mgig_Bot2012_COI	S15-03	1	BC25865_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25865	Sample	BC25865	targeted	Present	Sample	Mgig_Bot2012_COI	S15-03	COI	45	BC25865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.48451
BC25866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77797	WGS84	BC25866	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25866_Cmae_Bot2011_COI	S15-04	1	BC25866_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25866	Sample	BC25866	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-04	COI	45	BC25866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-04	ATGAACAGTCTATCCTCCTTTAG	138.5189
BC25868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77988	WGS84	BC25868	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25868_Aamu_Bax2006_COI	S15-06	1	BC25868_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25868	Sample	BC25868	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-06	COI	45	BC25868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-06	GCACAACCGGGATCTTTACTTC	138.51786
BC25868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77988	WGS84	BC25868	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25868_Pcan_Bot2011_IGS	S15-06	1	BC25868_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25868	Sample	BC25868	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-06	IGS	45	BC25868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-06	CGTAATCCTCAGTACTGGCTA	138.51786
BC25868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77988	WGS84	BC25868	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25868_Sspa_Oph2007_COI	S15-06	1	BC25868_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25868	Sample	BC25868	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-06	COI	45	BC25868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-06	GAAGGCCTTCCGATGATC	138.51786
BC25868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77988	WGS84	BC25868	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25868_Upin_Bot2011_COI	S15-06	1	BC25868_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25868	Sample	BC25868	targeted	Absent	Sample	Upin_Bot2011_COI	S15-06	COI	45	BC25868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.51786
BC25869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80875	WGS84	BC25869	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25869_Cjap_Sim2018_COI	S15-07	1	BC25869_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25869	Sample	BC25869	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-07	COI	45	BC25869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51685
BC25869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80875	WGS84	BC25869	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.94	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25869_Mgig_Bot2012_COI	S15-07	1	BC25869_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25869	Sample	BC25869	targeted	Present	Sample	Mgig_Bot2012_COI	S15-07	COI	45	BC25869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51685
BC25871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80946	WGS84	BC25871	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25871_Aamu_Bax2006_COI	S15-09	1	BC25871_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25871	Sample	BC25871	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-09	COI	45	BC25871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-09	GCACAACCGGGATCTTTACTTC	138.51863
BC25871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80946	WGS84	BC25871	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25871_Pcan_Bot2011_IGS	S15-09	1	BC25871_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25871	Sample	BC25871	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-09	IGS	45	BC25871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-09	CGTAATCCTCAGTACTGGCTA	138.51863
BC25871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80946	WGS84	BC25871	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25871_Sspa_Oph2007_COI	S15-09	1	BC25871_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25871	Sample	BC25871	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-09	COI	45	BC25871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-09	GAAGGCCTTCCGATGATC	138.51863
BC25871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80946	WGS84	BC25871	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25871_Upin_Bot2011_COI	S15-09	1	BC25871_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25871	Sample	BC25871	targeted	Absent	Sample	Upin_Bot2011_COI	S15-09	COI	45	BC25871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.51863
BC25872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83621	WGS84	BC25872	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25872_Cjap_Sim2018_COI	S15-10	1	BC25872_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25872	Sample	BC25872	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-10	COI	45	BC25872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50868
BC25872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83621	WGS84	BC25872	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.463	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25872_Mgig_Bot2012_COI	S15-10	1	BC25872_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25872	Sample	BC25872	targeted	Present	Sample	Mgig_Bot2012_COI	S15-10	COI	45	BC25872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.50868
BC25873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83683	WGS84	BC25873	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25873_Cmae_Bot2011_COI	S15-11	1	BC25873_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25873	Sample	BC25873	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-11	COI	45	BC25873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-11	ATGAACAGTCTATCCTCCTTTAG	138.5099
BC25875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84716	WGS84	BC25875	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25875_Aamu_Bax2006_COI	S15-13	1	BC25875_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25875	Sample	BC25875	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-13	COI	45	BC25875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-13	GCACAACCGGGATCTTTACTTC	138.49769
BC25875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84716	WGS84	BC25875	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25875_Pcan_Bot2011_IGS	S15-13	1	BC25875_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25875	Sample	BC25875	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-13	IGS	45	BC25875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-13	CGTAATCCTCAGTACTGGCTA	138.49769
BC25875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84716	WGS84	BC25875	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25875_Sspa_Oph2007_COI	S15-13	1	BC25875_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25875	Sample	BC25875	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-13	COI	45	BC25875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-13	GAAGGCCTTCCGATGATC	138.49769
BC25875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84716	WGS84	BC25875	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25875_Upin_Bot2011_COI	S15-13	1	BC25875_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25875	Sample	BC25875	targeted	Absent	Sample	Upin_Bot2011_COI	S15-13	COI	45	BC25875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.49769
BC25876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84566	WGS84	BC25876	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25876_Cjap_Sim2018_COI	S15-14	1	BC25876_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25876	Sample	BC25876	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-14	COI	45	BC25876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4971
BC25876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84566	WGS84	BC25876	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.893	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25876_Mgig_Bot2012_COI	S15-14	1	BC25876_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25876	Sample	BC25876	targeted	Present	Sample	Mgig_Bot2012_COI	S15-14	COI	45	BC25876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.4971
BC25877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84391	WGS84	BC25877	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25877_Cmae_Bot2011_COI	S15-15	1	BC25877_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25877	Sample	BC25877	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-15	COI	45	BC25877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-15	ATGAACAGTCTATCCTCCTTTAG	138.49688
BC25879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96277	WGS84	BC25879	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.199	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25879_Aamu_Bax2006_COI	T19-02	1	BC25879_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25879	Sample	BC25879	targeted	Present	Sample	Aamu_Bax2006_COI	T19-02	COI	45	BC25879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-02	GCACAACCGGGATCTTTACTTC	147.53956
BC25879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96277	WGS84	BC25879	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25879_Pcan_Bot2011_IGS	T19-02	1	BC25879_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25879	Sample	BC25879	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-02	IGS	45	BC25879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-02	CGTAATCCTCAGTACTGGCTA	147.53956
BC25879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96277	WGS84	BC25879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25879_Sspa_Oph2007_COI	T19-02	1	BC25879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25879	Sample	BC25879	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-02	COI	45	BC25879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-02	GAAGGCCTTCCGATGATC	147.53956
BC25879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96277	WGS84	BC25879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25879_Upin_Bot2011_COI	T19-02	1	BC25879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25879	Sample	BC25879	targeted	Absent	Sample	Upin_Bot2011_COI	T19-02	COI	45	BC25879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.53956
BC25880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.8101	WGS84	BC25880	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25880_Cmae_Bot2011_COI	T19-03	1	BC25880_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25880	Sample	BC25880	targeted	Absent	Sample	Cmae_Bot2011_COI	T19-03	COI	45	BC25880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-03	ATGAACAGTCTATCCTCCTTTAG	147.50248
BC25882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95965	WGS84	BC25882	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.073	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25882_Aamu_Bax2006_COI	T19-05	1	BC25882_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25882	Sample	BC25882	targeted	Present	Sample	Aamu_Bax2006_COI	T19-05	COI	45	BC25882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-05	GCACAACCGGGATCTTTACTTC	147.52786
BC25882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95965	WGS84	BC25882	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25882_Pcan_Bot2011_IGS	T19-05	1	BC25882_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25882	Sample	BC25882	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-05	IGS	45	BC25882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-05	CGTAATCCTCAGTACTGGCTA	147.52786
BC25882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95965	WGS84	BC25882	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25882_Sspa_Oph2007_COI	T19-05	1	BC25882_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25882	Sample	BC25882	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-05	COI	45	BC25882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-05	GAAGGCCTTCCGATGATC	147.52786
BC25882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95965	WGS84	BC25882	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25882_Upin_Bot2011_COI	T19-05	1	BC25882_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25882	Sample	BC25882	targeted	Absent	Sample	Upin_Bot2011_COI	T19-05	COI	45	BC25882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.52786
BC25883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96305	WGS84	BC25883	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25883_Cjap_Sim2018_COI	T19-06	1	BC25883_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25883	Sample	BC25883	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-06	COI	45	BC25883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53847
BC25883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96305	WGS84	BC25883	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.642	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	18/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25883_Mgig_Bot2012_COI	T19-06	1	BC25883_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25883	Sample	BC25883	targeted	Present	Sample	Mgig_Bot2012_COI	T19-06	COI	45	BC25883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.53847
BC25884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96014	WGS84	BC25884	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.544	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25884_Cmae_Bot2011_COI	T19-07	1	BC25884_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25884	Sample	BC25884	targeted	Present	Sample	Cmae_Bot2011_COI	T19-07	COI	45	BC25884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-07	ATGAACAGTCTATCCTCCTTTAG	147.53014
BC25886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96363	WGS84	BC25886	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.939	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25886_Aamu_Bax2006_COI	T19-09	1	BC25886_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25886	Sample	BC25886	targeted	Present	Sample	Aamu_Bax2006_COI	T19-09	COI	45	BC25886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-09	GCACAACCGGGATCTTTACTTC	147.51932
BC25886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96363	WGS84	BC25886	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25886_Pcan_Bot2011_IGS	T19-09	1	BC25886_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25886	Sample	BC25886	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-09	IGS	45	BC25886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-09	CGTAATCCTCAGTACTGGCTA	147.51932
BC25886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96363	WGS84	BC25886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25886_Sspa_Oph2007_COI	T19-09	1	BC25886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25886	Sample	BC25886	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-09	COI	45	BC25886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-09	GAAGGCCTTCCGATGATC	147.51932
BC25886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96363	WGS84	BC25886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25886_Upin_Bot2011_COI	T19-09	1	BC25886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25886	Sample	BC25886	targeted	Absent	Sample	Upin_Bot2011_COI	T19-09	COI	45	BC25886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.51932
BC25893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80378	WGS84	BC25893	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25893_Aamu_Bax2006_COI	T19-16	1	BC25893_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25893	Sample	BC25893	targeted	Absent	Sample	Aamu_Bax2006_COI	T19-16	COI	45	BC25893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-16	GCACAACCGGGATCTTTACTTC	147.5254
BC25893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80378	WGS84	BC25893	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25893_Pcan_Bot2011_IGS	T19-16	1	BC25893_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25893	Sample	BC25893	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-16	IGS	45	BC25893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-16	CGTAATCCTCAGTACTGGCTA	147.5254
BC25893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80378	WGS84	BC25893	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25893_Sspa_Oph2007_COI	T19-16	1	BC25893_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25893	Sample	BC25893	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-16	COI	45	BC25893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-16	GAAGGCCTTCCGATGATC	147.5254
BC25893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80378	WGS84	BC25893	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25893_Upin_Bot2011_COI	T19-16	1	BC25893_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25893	Sample	BC25893	targeted	Absent	Sample	Upin_Bot2011_COI	T19-16	COI	45	BC25893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.5254
BC25894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80318	WGS84	BC25894	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25894_Cjap_Sim2018_COI	T19-17	1	BC25894_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25894	Sample	BC25894	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-17	COI	45	BC25894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.46501
BC25894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80318	WGS84	BC25894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.206	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	17/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25894_Mgig_Bot2012_COI	T19-17	1	BC25894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25894	Sample	BC25894	targeted	Present	Sample	Mgig_Bot2012_COI	T19-17	COI	45	BC25894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-17	TCTTATTCGTTGGAGACTTTATAACCCT	147.46501
BC25895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25895_Cmae_Bot2011_COI	T19-18	1	BC25895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25895	Sample	BC25895	targeted	Absent	Sample	Cmae_Bot2011_COI	T19-18	COI	45	BC25895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-18	ATGAACAGTCTATCCTCCTTTAG	147.48557
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29597_Asen_Wil2025_COI	KEMB1	1	BC29597_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	GTTGTTGTTACAACACATGCTCTTTTG	150.90962
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29597_Mgig_Bot2012_COI	KEMB1	1	BC29597_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	TCTTATTCGTTGGAGACTTTATAACCCT	150.90962
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29598_Cmae_Bot2011_COI	KEMB2	1	BC29598_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	ATGAACAGTCTATCCTCCTTTAG	150.91231
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29598_Msal_Bot2012_COI	KEMB2	1	BC29598_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	GYTAGTTCCRATGATGTTAGCTG	150.91231
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29600_Aamu_Bax2006_COI	KEMB4	1	BC29600_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	GCACAACCGGGATCTTTACTTC	150.90917
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29600_Pcan_Bot2011_IGS	KEMB4	1	BC29600_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB4	IGS	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	CGTAATCCTCAGTACTGGCTA	150.90917
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29600_Sspa_Oph2007_COI	KEMB4	1	BC29600_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	GAAGGCCTTCCGATGATC	150.90917
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29600_Upin_Bot2011_COI	KEMB4	1	BC29600_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	CTTTAATTACAGCGTTTTTATTGTTGT	150.90917
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29600_Vgib_Wil2025_COI	KEMB4	1	BC29600_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	CCCCTCTTTACAAGTTCTTTAGGGT	150.90917
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29601_Asen_Wil2025_COI	KEMB5	1	BC29601_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	GTTGTTGTTACAACACATGCTCTTTTG	150.91196
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29601_Mgig_Bot2012_COI	KEMB5	1	BC29601_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	TCTTATTCGTTGGAGACTTTATAACCCT	150.91196
BC29602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88101	WGS84	BC29602	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29602_Cmae_Bot2011_COI	NEWA46	1	BC29602_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29602	Sample	BC29602	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA46	COI	45	BC29602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA46	ATGAACAGTCTATCCTCCTTTAG	151.75063
BC29602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88101	WGS84	BC29602	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29602_Msal_Bot2012_COI	NEWA46	1	BC29602_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29602	Sample	BC29602	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA46	COI	45	BC29602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA46	GYTAGTTCCRATGATGTTAGCTG	151.75063
BC29608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25322	WGS84	BC29608	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29608_Aamu_Bax2006_COI	HAYA80	1	BC29608_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29608	Sample	BC29608	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA80	COI	45	BC29608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA80	GCACAACCGGGATCTTTACTTC	149.30928
BC29608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25322	WGS84	BC29608	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29608_Pcan_Bot2011_IGS	HAYA80	1	BC29608_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29608	Sample	BC29608	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA80	IGS	45	BC29608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA80	CGTAATCCTCAGTACTGGCTA	149.30928
BC29608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25322	WGS84	BC29608	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29608_Sspa_Oph2007_COI	HAYA80	1	BC29608_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29608	Sample	BC29608	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA80	COI	45	BC29608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA80	GAAGGCCTTCCGATGATC	149.30928
BC29608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25322	WGS84	BC29608	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29608_Upin_Bot2011_COI	HAYA80	1	BC29608_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29608	Sample	BC29608	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA80	COI	45	BC29608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA80	CTTTAATTACAGCGTTTTTATTGTTGT	149.30928
BC29609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2534	WGS84	BC29609	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29609_Mgig_Bot2012_COI	HAYA79	1	BC29609_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29609	Sample	BC29609	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA79	COI	45	BC29609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA79	TCTTATTCGTTGGAGACTTTATAACCCT	149.30644
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.254	WGS84	BC29611	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29611_Aamu_Bax2006_COI	HAYA77	1	BC29611_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	GCACAACCGGGATCTTTACTTC	149.31117
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.254	WGS84	BC29611	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29611_Pcan_Bot2011_IGS	HAYA77	1	BC29611_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA77	IGS	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	CGTAATCCTCAGTACTGGCTA	149.31117
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.254	WGS84	BC29611	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29611_Sspa_Oph2007_COI	HAYA77	1	BC29611_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	GAAGGCCTTCCGATGATC	149.31117
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.254	WGS84	BC29611	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29611_Upin_Bot2011_COI	HAYA77	1	BC29611_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	CTTTAATTACAGCGTTTTTATTGTTGT	149.31117
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.254	WGS84	BC29611	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29611_Vgib_Wil2025_COI	HAYA77	1	BC29611_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	CCCCTCTTTACAAGTTCTTTAGGGT	149.31117
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25463	WGS84	BC29612	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29612_Asen_Wil2025_COI	HAYA76	1	BC29612_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	GTTGTTGTTACAACACATGCTCTTTTG	149.3096
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25463	WGS84	BC29612	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29612_Mgig_Bot2012_COI	HAYA76	1	BC29612_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	TCTTATTCGTTGGAGACTTTATAACCCT	149.3096
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25406	WGS84	BC29613	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29613_Cmae_Bot2011_COI	HAYA75	1	BC29613_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	ATGAACAGTCTATCCTCCTTTAG	149.30913
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25406	WGS84	BC29613	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29613_Msal_Bot2012_COI	HAYA75	1	BC29613_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	GYTAGTTCCRATGATGTTAGCTG	149.30913
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25489	WGS84	BC29615	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29615_Aamu_Bax2006_COI	HAYA73	1	BC29615_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	GCACAACCGGGATCTTTACTTC	149.30571
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25489	WGS84	BC29615	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29615_Pcan_Bot2011_IGS	HAYA73	1	BC29615_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA73	IGS	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	CGTAATCCTCAGTACTGGCTA	149.30571
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25489	WGS84	BC29615	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29615_Sspa_Oph2007_COI	HAYA73	1	BC29615_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	GAAGGCCTTCCGATGATC	149.30571
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25489	WGS84	BC29615	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29615_Upin_Bot2011_COI	HAYA73	1	BC29615_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	CTTTAATTACAGCGTTTTTATTGTTGT	149.30571
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25489	WGS84	BC29615	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29615_Vgib_Wil2025_COI	HAYA73	1	BC29615_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	CCCCTCTTTACAAGTTCTTTAGGGT	149.30571
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25433	WGS84	BC29616	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29616_Asen_Wil2025_COI	HAYA72	1	BC29616_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	GTTGTTGTTACAACACATGCTCTTTTG	149.31174
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25433	WGS84	BC29616	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29616_Mgig_Bot2012_COI	HAYA72	1	BC29616_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	TCTTATTCGTTGGAGACTTTATAACCCT	149.31174
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25514	WGS84	BC29617	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29617_Cmae_Bot2011_COI	HAYA71	1	BC29617_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	ATGAACAGTCTATCCTCCTTTAG	149.30984
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25514	WGS84	BC29617	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29617_Msal_Bot2012_COI	HAYA71	1	BC29617_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	GYTAGTTCCRATGATGTTAGCTG	149.30984
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25577	WGS84	BC29619	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29619_Aamu_Bax2006_COI	HAYA69	1	BC29619_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	GCACAACCGGGATCTTTACTTC	149.30652
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25577	WGS84	BC29619	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29619_Pcan_Bot2011_IGS	HAYA69	1	BC29619_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA69	IGS	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	CGTAATCCTCAGTACTGGCTA	149.30652
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25577	WGS84	BC29619	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29619_Sspa_Oph2007_COI	HAYA69	1	BC29619_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	GAAGGCCTTCCGATGATC	149.30652
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25577	WGS84	BC29619	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29619_Upin_Bot2011_COI	HAYA69	1	BC29619_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	CTTTAATTACAGCGTTTTTATTGTTGT	149.30652
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25577	WGS84	BC29619	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29619_Vgib_Wil2025_COI	HAYA69	1	BC29619_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	CCCCTCTTTACAAGTTCTTTAGGGT	149.30652
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25554	WGS84	BC29620	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29620_Cmae_Bot2011_COI	HAYA68	1	BC29620_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	ATGAACAGTCTATCCTCCTTTAG	149.31215
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25554	WGS84	BC29620	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29620_Msal_Bot2012_COI	HAYA68	1	BC29620_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	GYTAGTTCCRATGATGTTAGCTG	149.31215
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25536	WGS84	BC29622	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29622_Aamu_Bax2006_COI	HAYA66	1	BC29622_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	GCACAACCGGGATCTTTACTTC	149.3102
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25536	WGS84	BC29622	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29622_Pcan_Bot2011_IGS	HAYA66	1	BC29622_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA66	IGS	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	CGTAATCCTCAGTACTGGCTA	149.3102
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25536	WGS84	BC29622	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29622_Sspa_Oph2007_COI	HAYA66	1	BC29622_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	GAAGGCCTTCCGATGATC	149.3102
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25536	WGS84	BC29622	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29622_Upin_Bot2011_COI	HAYA66	1	BC29622_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	CTTTAATTACAGCGTTTTTATTGTTGT	149.3102
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25536	WGS84	BC29622	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29622_Vgib_Wil2025_COI	HAYA66	1	BC29622_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	CCCCTCTTTACAAGTTCTTTAGGGT	149.3102
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25552	WGS84	BC29623	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29623_Asen_Wil2025_COI	HAYA65	1	BC29623_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	GTTGTTGTTACAACACATGCTCTTTTG	149.30799
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25552	WGS84	BC29623	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29623_Mgig_Bot2012_COI	HAYA65	1	BC29623_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	TCTTATTCGTTGGAGACTTTATAACCCT	149.30799
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25631	WGS84	BC29624	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29624_Cmae_Bot2011_COI	HAYA64	1	BC29624_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	ATGAACAGTCTATCCTCCTTTAG	149.30702
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25631	WGS84	BC29624	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29624_Msal_Bot2012_COI	HAYA64	1	BC29624_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	GYTAGTTCCRATGATGTTAGCTG	149.30702
BC29626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25676	WGS84	BC29626	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29626_Aamu_Bax2006_COI	HAYA62	1	BC29626_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29626	Sample	BC29626	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA62	COI	45	BC29626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA62	GCACAACCGGGATCTTTACTTC	149.31127
BC29626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25676	WGS84	BC29626	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29626_Pcan_Bot2011_IGS	HAYA62	1	BC29626_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29626	Sample	BC29626	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA62	IGS	45	BC29626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA62	CGTAATCCTCAGTACTGGCTA	149.31127
BC29626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25676	WGS84	BC29626	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29626_Sspa_Oph2007_COI	HAYA62	1	BC29626_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29626	Sample	BC29626	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA62	COI	45	BC29626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA62	GAAGGCCTTCCGATGATC	149.31127
BC29626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25676	WGS84	BC29626	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29626_Upin_Bot2011_COI	HAYA62	1	BC29626_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29626	Sample	BC29626	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA62	COI	45	BC29626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA62	CTTTAATTACAGCGTTTTTATTGTTGT	149.31127
BC29627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25593	WGS84	BC29627	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29627_Mgig_Bot2012_COI	HAYA61	1	BC29627_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29627	Sample	BC29627	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA61	COI	45	BC29627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA61	TCTTATTCGTTGGAGACTTTATAACCCT	149.31054
BC29628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25702	WGS84	BC29628	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29628_Cmae_Bot2011_COI	HAYA60	1	BC29628_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29628	Sample	BC29628	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA60	COI	45	BC29628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA60	ATGAACAGTCTATCCTCCTTTAG	149.30984
BC29628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25702	WGS84	BC29628	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29628_Msal_Bot2012_COI	HAYA60	1	BC29628_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29628	Sample	BC29628	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA60	COI	45	BC29628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA60	GYTAGTTCCRATGATGTTAGCTG	149.30984
BC29630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2565	WGS84	BC29630	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29630_Mgig_Bot2012_COI	HAYA58	1	BC29630_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29630	Sample	BC29630	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA58	COI	45	BC29630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA58	TCTTATTCGTTGGAGACTTTATAACCCT	149.30741
BC29631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25743	WGS84	BC29631	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29631_Cmae_Bot2011_COI	HAYA57	1	BC29631_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29631	Sample	BC29631	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA57	COI	45	BC29631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA57	ATGAACAGTCTATCCTCCTTTAG	149.3123
BC29631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25743	WGS84	BC29631	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29631_Msal_Bot2012_COI	HAYA57	1	BC29631_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29631	Sample	BC29631	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA57	COI	45	BC29631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA57	GYTAGTTCCRATGATGTTAGCTG	149.3123
BC29633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25669	WGS84	BC29633	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29633_Aamu_Bax2006_COI	HAYA55	1	BC29633_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29633	Sample	BC29633	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA55	COI	45	BC29633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA55	GCACAACCGGGATCTTTACTTC	149.31006
BC29633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25669	WGS84	BC29633	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29633_Pcan_Bot2011_IGS	HAYA55	1	BC29633_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29633	Sample	BC29633	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA55	IGS	45	BC29633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA55	CGTAATCCTCAGTACTGGCTA	149.31006
BC29633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25669	WGS84	BC29633	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29633_Sspa_Oph2007_COI	HAYA55	1	BC29633_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29633	Sample	BC29633	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA55	COI	45	BC29633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA55	GAAGGCCTTCCGATGATC	149.31006
BC29633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25669	WGS84	BC29633	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29633_Upin_Bot2011_COI	HAYA55	1	BC29633_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29633	Sample	BC29633	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA55	COI	45	BC29633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA55	CTTTAATTACAGCGTTTTTATTGTTGT	149.31006
BC29634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25781	WGS84	BC29634	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29634_Mgig_Bot2012_COI	HAYA54	1	BC29634_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29634	Sample	BC29634	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA54	COI	45	BC29634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA54	TCTTATTCGTTGGAGACTTTATAACCCT	149.30938
BC29635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25792	WGS84	BC29635	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29635_Cmae_Bot2011_COI	HAYA53	1	BC29635_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29635	Sample	BC29635	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA53	COI	45	BC29635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA53	ATGAACAGTCTATCCTCCTTTAG	149.30823
BC29635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25792	WGS84	BC29635	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29635_Msal_Bot2012_COI	HAYA53	1	BC29635_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29635	Sample	BC29635	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA53	COI	45	BC29635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA53	GYTAGTTCCRATGATGTTAGCTG	149.30823
BC29637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25823	WGS84	BC29637	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29637_Aamu_Bax2006_COI	HAYA51	1	BC29637_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29637	Sample	BC29637	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA51	COI	45	BC29637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA51	GCACAACCGGGATCTTTACTTC	149.31177
BC29637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25823	WGS84	BC29637	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29637_Pcan_Bot2011_IGS	HAYA51	1	BC29637_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29637	Sample	BC29637	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA51	IGS	45	BC29637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA51	CGTAATCCTCAGTACTGGCTA	149.31177
BC29637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25823	WGS84	BC29637	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29637_Sspa_Oph2007_COI	HAYA51	1	BC29637_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29637	Sample	BC29637	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA51	COI	45	BC29637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA51	GAAGGCCTTCCGATGATC	149.31177
BC29637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25823	WGS84	BC29637	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29637_Upin_Bot2011_COI	HAYA51	1	BC29637_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29637	Sample	BC29637	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA51	COI	45	BC29637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA51	CTTTAATTACAGCGTTTTTATTGTTGT	149.31177
BC29638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25789	WGS84	BC29638	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29638_Mgig_Bot2012_COI	HAYA50	1	BC29638_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29638	Sample	BC29638	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA50	COI	45	BC29638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA50	TCTTATTCGTTGGAGACTTTATAACCCT	149.30852
BC29639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25811	WGS84	BC29639	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29639_Cmae_Bot2011_COI	HAYA49	1	BC29639_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29639	Sample	BC29639	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA49	COI	45	BC29639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA49	ATGAACAGTCTATCCTCCTTTAG	149.31273
BC29639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25811	WGS84	BC29639	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29639_Msal_Bot2012_COI	HAYA49	1	BC29639_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29639	Sample	BC29639	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA49	COI	45	BC29639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA49	GYTAGTTCCRATGATGTTAGCTG	149.31273
BC29640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25897	WGS84	BC29640	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29640_Aamu_Bax2006_COI	HAYA48	1	BC29640_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29640	Sample	BC29640	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA48	COI	45	BC29640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA48	GCACAACCGGGATCTTTACTTC	149.31222
BC29640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25897	WGS84	BC29640	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29640_Pcan_Bot2011_IGS	HAYA48	1	BC29640_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29640	Sample	BC29640	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA48	IGS	45	BC29640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA48	CGTAATCCTCAGTACTGGCTA	149.31222
BC29640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25897	WGS84	BC29640	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29640_Sspa_Oph2007_COI	HAYA48	1	BC29640_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29640	Sample	BC29640	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA48	COI	45	BC29640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA48	GAAGGCCTTCCGATGATC	149.31222
BC29640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25897	WGS84	BC29640	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29640_Upin_Bot2011_COI	HAYA48	1	BC29640_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29640	Sample	BC29640	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA48	COI	45	BC29640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA48	CTTTAATTACAGCGTTTTTATTGTTGT	149.31222
BC29641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25844	WGS84	BC29641	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29641_Mgig_Bot2012_COI	HAYA47	1	BC29641_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29641	Sample	BC29641	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA47	COI	45	BC29641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA47	TCTTATTCGTTGGAGACTTTATAACCCT	149.30923
BC29642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25917	WGS84	BC29642	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29642_Cmae_Bot2011_COI	HAYA46	1	BC29642_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29642	Sample	BC29642	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA46	COI	45	BC29642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA46	ATGAACAGTCTATCCTCCTTTAG	149.31302
BC29642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25917	WGS84	BC29642	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29642_Msal_Bot2012_COI	HAYA46	1	BC29642_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29642	Sample	BC29642	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA46	COI	45	BC29642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA46	GYTAGTTCCRATGATGTTAGCTG	149.31302
BC29644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25974	WGS84	BC29644	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29644_Aamu_Bax2006_COI	HAYA44	1	BC29644_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29644	Sample	BC29644	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA44	COI	45	BC29644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA44	GCACAACCGGGATCTTTACTTC	149.31053
BC29644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25974	WGS84	BC29644	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29644_Pcan_Bot2011_IGS	HAYA44	1	BC29644_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29644	Sample	BC29644	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA44	IGS	45	BC29644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA44	CGTAATCCTCAGTACTGGCTA	149.31053
BC29644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25974	WGS84	BC29644	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29644_Sspa_Oph2007_COI	HAYA44	1	BC29644_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29644	Sample	BC29644	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA44	COI	45	BC29644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA44	GAAGGCCTTCCGATGATC	149.31053
BC29644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25974	WGS84	BC29644	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29644_Upin_Bot2011_COI	HAYA44	1	BC29644_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29644	Sample	BC29644	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA44	COI	45	BC29644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA44	CTTTAATTACAGCGTTTTTATTGTTGT	149.31053
BC29645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25954	WGS84	BC29645	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29645_Mgig_Bot2012_COI	HAYA43	1	BC29645_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29645	Sample	BC29645	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA43	COI	45	BC29645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA43	TCTTATTCGTTGGAGACTTTATAACCCT	149.30874
BC29646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25938	WGS84	BC29646	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29646_Cmae_Bot2011_COI	HAYA42	1	BC29646_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29646	Sample	BC29646	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA42	COI	45	BC29646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA42	ATGAACAGTCTATCCTCCTTTAG	149.3134
BC29646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25938	WGS84	BC29646	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29646_Msal_Bot2012_COI	HAYA42	1	BC29646_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29646	Sample	BC29646	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA42	COI	45	BC29646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA42	GYTAGTTCCRATGATGTTAGCTG	149.3134
BC29648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25966	WGS84	BC29648	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29648_Aamu_Bax2006_COI	HAYA40	1	BC29648_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29648	Sample	BC29648	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA40	COI	45	BC29648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA40	GCACAACCGGGATCTTTACTTC	149.31048
BC29648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25966	WGS84	BC29648	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29648_Pcan_Bot2011_IGS	HAYA40	1	BC29648_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29648	Sample	BC29648	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA40	IGS	45	BC29648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA40	CGTAATCCTCAGTACTGGCTA	149.31048
BC29648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25966	WGS84	BC29648	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29648_Sspa_Oph2007_COI	HAYA40	1	BC29648_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29648	Sample	BC29648	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA40	COI	45	BC29648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA40	GAAGGCCTTCCGATGATC	149.31048
BC29648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25966	WGS84	BC29648	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29648_Upin_Bot2011_COI	HAYA40	1	BC29648_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29648	Sample	BC29648	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA40	COI	45	BC29648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA40	CTTTAATTACAGCGTTTTTATTGTTGT	149.31048
BC29649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26015	WGS84	BC29649	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29649_Mgig_Bot2012_COI	HAYA39	1	BC29649_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29649	Sample	BC29649	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA39	COI	45	BC29649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA39	TCTTATTCGTTGGAGACTTTATAACCCT	149.30916
BC29651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26007	WGS84	BC29651	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29651_Aamu_Bax2006_COI	HAYA37	1	BC29651_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29651	Sample	BC29651	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA37	COI	45	BC29651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA37	GCACAACCGGGATCTTTACTTC	149.31273
BC29651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26007	WGS84	BC29651	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29651_Pcan_Bot2011_IGS	HAYA37	1	BC29651_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29651	Sample	BC29651	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA37	IGS	45	BC29651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA37	CGTAATCCTCAGTACTGGCTA	149.31273
BC29651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26007	WGS84	BC29651	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29651_Sspa_Oph2007_COI	HAYA37	1	BC29651_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29651	Sample	BC29651	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA37	COI	45	BC29651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA37	GAAGGCCTTCCGATGATC	149.31273
BC29651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26007	WGS84	BC29651	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29651_Upin_Bot2011_COI	HAYA37	1	BC29651_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29651	Sample	BC29651	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA37	COI	45	BC29651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA37	CTTTAATTACAGCGTTTTTATTGTTGT	149.31273
BC29652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26079	WGS84	BC29652	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29652_Mgig_Bot2012_COI	HAYA36	1	BC29652_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29652	Sample	BC29652	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA36	COI	45	BC29652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA36	TCTTATTCGTTGGAGACTTTATAACCCT	149.31151
BC29653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26043	WGS84	BC29653	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29653_Cmae_Bot2011_COI	HAYA35	1	BC29653_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29653	Sample	BC29653	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA35	COI	45	BC29653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA35	ATGAACAGTCTATCCTCCTTTAG	149.30992
BC29653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26043	WGS84	BC29653	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29653_Msal_Bot2012_COI	HAYA35	1	BC29653_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29653	Sample	BC29653	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA35	COI	45	BC29653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA35	GYTAGTTCCRATGATGTTAGCTG	149.30992
BC29655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26145	WGS84	BC29655	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29655_Aamu_Bax2006_COI	HAYA33	1	BC29655_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29655	Sample	BC29655	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA33	COI	45	BC29655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA33	GCACAACCGGGATCTTTACTTC	149.31383
BC29655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26145	WGS84	BC29655	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29655_Pcan_Bot2011_IGS	HAYA33	1	BC29655_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29655	Sample	BC29655	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA33	IGS	45	BC29655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA33	CGTAATCCTCAGTACTGGCTA	149.31383
BC29655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26145	WGS84	BC29655	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29655_Sspa_Oph2007_COI	HAYA33	1	BC29655_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29655	Sample	BC29655	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA33	COI	45	BC29655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA33	GAAGGCCTTCCGATGATC	149.31383
BC29655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26145	WGS84	BC29655	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29655_Upin_Bot2011_COI	HAYA33	1	BC29655_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29655	Sample	BC29655	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA33	COI	45	BC29655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA33	CTTTAATTACAGCGTTTTTATTGTTGT	149.31383
BC29656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26191	WGS84	BC29656	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29656_Mgig_Bot2012_COI	HAYA32	1	BC29656_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29656	Sample	BC29656	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA32	COI	45	BC29656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA32	TCTTATTCGTTGGAGACTTTATAACCCT	149.31036
BC29657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26186	WGS84	BC29657	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29657_Cmae_Bot2011_COI	HAYA31	1	BC29657_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29657	Sample	BC29657	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA31	COI	45	BC29657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA31	ATGAACAGTCTATCCTCCTTTAG	149.3153
BC29657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26186	WGS84	BC29657	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29657_Msal_Bot2012_COI	HAYA31	1	BC29657_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29657	Sample	BC29657	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA31	COI	45	BC29657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA31	GYTAGTTCCRATGATGTTAGCTG	149.3153
BC29659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26259	WGS84	BC29659	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29659_Aamu_Bax2006_COI	HAYA29	1	BC29659_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29659	Sample	BC29659	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA29	COI	45	BC29659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA29	GCACAACCGGGATCTTTACTTC	149.31503
BC29659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26259	WGS84	BC29659	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29659_Pcan_Bot2011_IGS	HAYA29	1	BC29659_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29659	Sample	BC29659	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA29	IGS	45	BC29659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA29	CGTAATCCTCAGTACTGGCTA	149.31503
BC29659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26259	WGS84	BC29659	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29659_Sspa_Oph2007_COI	HAYA29	1	BC29659_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29659	Sample	BC29659	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA29	COI	45	BC29659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA29	GAAGGCCTTCCGATGATC	149.31503
BC29659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26259	WGS84	BC29659	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29659_Upin_Bot2011_COI	HAYA29	1	BC29659_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29659	Sample	BC29659	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA29	COI	45	BC29659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA29	CTTTAATTACAGCGTTTTTATTGTTGT	149.31503
BC29660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26306	WGS84	BC29660	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29660_Cmae_Bot2011_COI	HAYA28	1	BC29660_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29660	Sample	BC29660	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA28	COI	45	BC29660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA28	ATGAACAGTCTATCCTCCTTTAG	149.31389
BC29660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26306	WGS84	BC29660	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29660_Msal_Bot2012_COI	HAYA28	1	BC29660_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29660	Sample	BC29660	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA28	COI	45	BC29660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA28	GYTAGTTCCRATGATGTTAGCTG	149.31389
BC29662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26291	WGS84	BC29662	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29662_Aamu_Bax2006_COI	HAYA26	1	BC29662_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29662	Sample	BC29662	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA26	COI	45	BC29662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA26	GCACAACCGGGATCTTTACTTC	149.31415
BC29662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26291	WGS84	BC29662	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29662_Pcan_Bot2011_IGS	HAYA26	1	BC29662_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29662	Sample	BC29662	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA26	IGS	45	BC29662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA26	CGTAATCCTCAGTACTGGCTA	149.31415
BC29662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26291	WGS84	BC29662	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29662_Sspa_Oph2007_COI	HAYA26	1	BC29662_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29662	Sample	BC29662	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA26	COI	45	BC29662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA26	GAAGGCCTTCCGATGATC	149.31415
BC29662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26291	WGS84	BC29662	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29662_Upin_Bot2011_COI	HAYA26	1	BC29662_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29662	Sample	BC29662	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA26	COI	45	BC29662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA26	CTTTAATTACAGCGTTTTTATTGTTGT	149.31415
BC29663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26424	WGS84	BC29663	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29663_Mgig_Bot2012_COI	HAYA25	1	BC29663_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29663	Sample	BC29663	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA25	COI	45	BC29663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA25	TCTTATTCGTTGGAGACTTTATAACCCT	149.3158
BC29664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26401	WGS84	BC29664	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29664_Cmae_Bot2011_COI	HAYA24	1	BC29664_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29664	Sample	BC29664	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA24	COI	45	BC29664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA24	ATGAACAGTCTATCCTCCTTTAG	149.31418
BC29664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26401	WGS84	BC29664	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29664_Msal_Bot2012_COI	HAYA24	1	BC29664_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29664	Sample	BC29664	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA24	COI	45	BC29664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA24	GYTAGTTCCRATGATGTTAGCTG	149.31418
BC29666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26398	WGS84	BC29666	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29666_Aamu_Bax2006_COI	HAYA22	1	BC29666_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29666	Sample	BC29666	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA22	COI	45	BC29666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA22	GCACAACCGGGATCTTTACTTC	149.31106
BC29666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26398	WGS84	BC29666	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29666_Pcan_Bot2011_IGS	HAYA22	1	BC29666_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29666	Sample	BC29666	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA22	IGS	45	BC29666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA22	CGTAATCCTCAGTACTGGCTA	149.31106
BC29666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26398	WGS84	BC29666	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29666_Sspa_Oph2007_COI	HAYA22	1	BC29666_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29666	Sample	BC29666	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA22	COI	45	BC29666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA22	GAAGGCCTTCCGATGATC	149.31106
BC29666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26398	WGS84	BC29666	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29666_Upin_Bot2011_COI	HAYA22	1	BC29666_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29666	Sample	BC29666	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA22	COI	45	BC29666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA22	CTTTAATTACAGCGTTTTTATTGTTGT	149.31106
BC29667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26507	WGS84	BC29667	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29667_Mgig_Bot2012_COI	HAYA21	1	BC29667_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29667	Sample	BC29667	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA21	COI	45	BC29667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA21	TCTTATTCGTTGGAGACTTTATAACCCT	149.31557
BC29668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26436	WGS84	BC29668	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29668_Cmae_Bot2011_COI	HAYA20	1	BC29668_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29668	Sample	BC29668	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA20	COI	45	BC29668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA20	ATGAACAGTCTATCCTCCTTTAG	149.31409
BC29668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26436	WGS84	BC29668	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29668_Msal_Bot2012_COI	HAYA20	1	BC29668_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29668	Sample	BC29668	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA20	COI	45	BC29668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA20	GYTAGTTCCRATGATGTTAGCTG	149.31409
BC29670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26505	WGS84	BC29670	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29670_Mgig_Bot2012_COI	HAYA18	1	BC29670_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29670	Sample	BC29670	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA18	COI	45	BC29670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA18	TCTTATTCGTTGGAGACTTTATAACCCT	149.31493
BC29671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26574	WGS84	BC29671	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29671_Cmae_Bot2011_COI	HAYA17	1	BC29671_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29671	Sample	BC29671	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA17	COI	45	BC29671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA17	ATGAACAGTCTATCCTCCTTTAG	149.31447
BC29671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26574	WGS84	BC29671	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29671_Msal_Bot2012_COI	HAYA17	1	BC29671_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29671	Sample	BC29671	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA17	COI	45	BC29671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA17	GYTAGTTCCRATGATGTTAGCTG	149.31447
BC29673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26654	WGS84	BC29673	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29673_Aamu_Bax2006_COI	HAYA15	1	BC29673_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29673	Sample	BC29673	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA15	COI	45	BC29673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA15	GCACAACCGGGATCTTTACTTC	149.31624
BC29673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26654	WGS84	BC29673	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29673_Pcan_Bot2011_IGS	HAYA15	1	BC29673_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29673	Sample	BC29673	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA15	IGS	45	BC29673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA15	CGTAATCCTCAGTACTGGCTA	149.31624
BC29673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26654	WGS84	BC29673	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29673_Sspa_Oph2007_COI	HAYA15	1	BC29673_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29673	Sample	BC29673	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA15	COI	45	BC29673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA15	GAAGGCCTTCCGATGATC	149.31624
BC29673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26654	WGS84	BC29673	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29673_Upin_Bot2011_COI	HAYA15	1	BC29673_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29673	Sample	BC29673	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA15	COI	45	BC29673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA15	CTTTAATTACAGCGTTTTTATTGTTGT	149.31624
BC29674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26576	WGS84	BC29674	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29674_Mgig_Bot2012_COI	HAYA14	1	BC29674_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29674	Sample	BC29674	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA14	COI	45	BC29674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA14	TCTTATTCGTTGGAGACTTTATAACCCT	149.31553
BC29675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29675	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29675_Cmae_Bot2011_COI	HAYA13	1	BC29675_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29675	Sample	BC29675	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA13	COI	45	BC29675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA13	ATGAACAGTCTATCCTCCTTTAG	149.31476
BC29675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29675	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29675_Msal_Bot2012_COI	HAYA13	1	BC29675_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29675	Sample	BC29675	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA13	COI	45	BC29675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA13	GYTAGTTCCRATGATGTTAGCTG	149.31476
BC29677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26725	WGS84	BC29677	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29677_Aamu_Bax2006_COI	HAYA11	1	BC29677_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29677	Sample	BC29677	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA11	COI	45	BC29677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA11	GCACAACCGGGATCTTTACTTC	149.31629
BC29677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26725	WGS84	BC29677	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29677_Pcan_Bot2011_IGS	HAYA11	1	BC29677_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29677	Sample	BC29677	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA11	IGS	45	BC29677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA11	CGTAATCCTCAGTACTGGCTA	149.31629
BC29677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26725	WGS84	BC29677	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29677_Sspa_Oph2007_COI	HAYA11	1	BC29677_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29677	Sample	BC29677	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA11	COI	45	BC29677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA11	GAAGGCCTTCCGATGATC	149.31629
BC29677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26725	WGS84	BC29677	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29677_Upin_Bot2011_COI	HAYA11	1	BC29677_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29677	Sample	BC29677	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA11	COI	45	BC29677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA11	CTTTAATTACAGCGTTTTTATTGTTGT	149.31629
BC29678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26727	WGS84	BC29678	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29678_Mgig_Bot2012_COI	HAYA10	1	BC29678_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29678	Sample	BC29678	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA10	COI	45	BC29678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA10	TCTTATTCGTTGGAGACTTTATAACCCT	149.31534
BC29679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2668	WGS84	BC29679	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29679_Cmae_Bot2011_COI	HAYA9	1	BC29679_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29679	Sample	BC29679	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA9	COI	45	BC29679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA9	ATGAACAGTCTATCCTCCTTTAG	149.31294
BC29679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2668	WGS84	BC29679	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29679_Msal_Bot2012_COI	HAYA9	1	BC29679_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29679	Sample	BC29679	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA9	COI	45	BC29679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA9	GYTAGTTCCRATGATGTTAGCTG	149.31294
BC29680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26761	WGS84	BC29680	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29680_Aamu_Bax2006_COI	HAYA8	1	BC29680_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29680	Sample	BC29680	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA8	COI	45	BC29680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA8	GCACAACCGGGATCTTTACTTC	149.31205
BC29680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26761	WGS84	BC29680	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29680_Pcan_Bot2011_IGS	HAYA8	1	BC29680_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29680	Sample	BC29680	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA8	IGS	45	BC29680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA8	CGTAATCCTCAGTACTGGCTA	149.31205
BC29680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26761	WGS84	BC29680	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29680_Sspa_Oph2007_COI	HAYA8	1	BC29680_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29680	Sample	BC29680	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA8	COI	45	BC29680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA8	GAAGGCCTTCCGATGATC	149.31205
BC29680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26761	WGS84	BC29680	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29680_Upin_Bot2011_COI	HAYA8	1	BC29680_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29680	Sample	BC29680	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA8	COI	45	BC29680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA8	CTTTAATTACAGCGTTTTTATTGTTGT	149.31205
BC29681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26785	WGS84	BC29681	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29681_Mgig_Bot2012_COI	HAYA7	1	BC29681_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29681	Sample	BC29681	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA7	COI	45	BC29681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA7	TCTTATTCGTTGGAGACTTTATAACCCT	149.31684
BC29682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26714	WGS84	BC29682	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29682_Cmae_Bot2011_COI	HAYA6	1	BC29682_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29682	Sample	BC29682	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA6	COI	45	BC29682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA6	ATGAACAGTCTATCCTCCTTTAG	149.31552
BC29682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26714	WGS84	BC29682	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29682_Msal_Bot2012_COI	HAYA6	1	BC29682_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29682	Sample	BC29682	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA6	COI	45	BC29682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA6	GYTAGTTCCRATGATGTTAGCTG	149.31552
BC29684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26749	WGS84	BC29684	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29684_Aamu_Bax2006_COI	HAYA4	1	BC29684_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29684	Sample	BC29684	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA4	COI	45	BC29684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA4	GCACAACCGGGATCTTTACTTC	149.3128
BC29684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26749	WGS84	BC29684	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29684_Pcan_Bot2011_IGS	HAYA4	1	BC29684_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29684	Sample	BC29684	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA4	IGS	45	BC29684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA4	CGTAATCCTCAGTACTGGCTA	149.3128
BC29684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26749	WGS84	BC29684	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29684_Sspa_Oph2007_COI	HAYA4	1	BC29684_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29684	Sample	BC29684	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA4	COI	45	BC29684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA4	GAAGGCCTTCCGATGATC	149.3128
BC29684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26749	WGS84	BC29684	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29684_Upin_Bot2011_COI	HAYA4	1	BC29684_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29684	Sample	BC29684	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA4	COI	45	BC29684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA4	CTTTAATTACAGCGTTTTTATTGTTGT	149.3128
BC29685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26771	WGS84	BC29685	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29685_Mgig_Bot2012_COI	HAYA3	1	BC29685_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29685	Sample	BC29685	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA3	COI	45	BC29685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA3	TCTTATTCGTTGGAGACTTTATAACCCT	149.31608
BC29686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26894	WGS84	BC29686	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29686_Cmae_Bot2011_COI	HAYA2	1	BC29686_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29686	Sample	BC29686	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA2	COI	45	BC29686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA2	ATGAACAGTCTATCCTCCTTTAG	149.31393
BC29686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26894	WGS84	BC29686	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29686_Msal_Bot2012_COI	HAYA2	1	BC29686_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29686	Sample	BC29686	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA2	COI	45	BC29686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA2	GYTAGTTCCRATGATGTTAGCTG	149.31393
BC29688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18009	WGS84	BC29688	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29688_Aamu_Bax2006_COI	DEVA19	1	BC29688_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29688	Sample	BC29688	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA19	COI	45	BC29688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA19	GCACAACCGGGATCTTTACTTC	146.3645
BC29688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18009	WGS84	BC29688	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29688_Pcan_Bot2011_IGS	DEVA19	1	BC29688_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29688	Sample	BC29688	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA19	IGS	45	BC29688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA19	CGTAATCCTCAGTACTGGCTA	146.3645
BC29688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18009	WGS84	BC29688	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29688_Sspa_Oph2007_COI	DEVA19	1	BC29688_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29688	Sample	BC29688	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA19	COI	45	BC29688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA19	GAAGGCCTTCCGATGATC	146.3645
BC29688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18009	WGS84	BC29688	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29688_Upin_Bot2011_COI	DEVA19	1	BC29688_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29688	Sample	BC29688	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA19	COI	45	BC29688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA19	CTTTAATTACAGCGTTTTTATTGTTGT	146.3645
BC29689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18175	WGS84	BC29689	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.752	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29689_Mgig_Bot2012_COI	DEVA18	1	BC29689_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29689	Sample	BC29689	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA18	COI	45	BC29689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA18	TCTTATTCGTTGGAGACTTTATAACCCT	146.36453
BC29692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18176	WGS84	BC29692	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.532	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29692_Mgig_Bot2012_COI	DEVA16	1	BC29692_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29692	Sample	BC29692	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA16	COI	45	BC29692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA16	TCTTATTCGTTGGAGACTTTATAACCCT	146.36377
BC29693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1824	WGS84	BC29693	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29693_Cmae_Bot2011_COI	DEVA15	1	BC29693_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29693	Sample	BC29693	targeted	Absent	Sample	Cmae_Bot2011_COI	DEVA15	COI	45	BC29693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA15	ATGAACAGTCTATCCTCCTTTAG	146.36585
BC29693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1824	WGS84	BC29693	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29693_Msal_Bot2012_COI	DEVA15	1	BC29693_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29693	Sample	BC29693	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA15	COI	45	BC29693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA15	GYTAGTTCCRATGATGTTAGCTG	146.36585
BC29695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18368	WGS84	BC29695	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29695_Aamu_Bax2006_COI	DEVA12	1	BC29695_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29695	Sample	BC29695	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA12	COI	45	BC29695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA12	GCACAACCGGGATCTTTACTTC	146.36384
BC29695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18368	WGS84	BC29695	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29695_Pcan_Bot2011_IGS	DEVA12	1	BC29695_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29695	Sample	BC29695	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA12	IGS	45	BC29695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA12	CGTAATCCTCAGTACTGGCTA	146.36384
BC29695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18368	WGS84	BC29695	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29695_Sspa_Oph2007_COI	DEVA12	1	BC29695_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29695	Sample	BC29695	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA12	COI	45	BC29695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA12	GAAGGCCTTCCGATGATC	146.36384
BC29695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18368	WGS84	BC29695	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29695_Upin_Bot2011_COI	DEVA12	1	BC29695_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29695	Sample	BC29695	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA12	COI	45	BC29695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA12	CTTTAATTACAGCGTTTTTATTGTTGT	146.36384
BC29696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18545	WGS84	BC29696	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.666	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29696_Mgig_Bot2012_COI	DEVA10	1	BC29696_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29696	Sample	BC29696	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA10	COI	45	BC29696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA10	TCTTATTCGTTGGAGACTTTATAACCCT	146.36573
BC29697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18569	WGS84	BC29697	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.367	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29697_Cmae_Bot2011_COI	DEVA9	1	BC29697_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29697	Sample	BC29697	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA9	COI	45	BC29697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA9	ATGAACAGTCTATCCTCCTTTAG	146.36387
BC29697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18569	WGS84	BC29697	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29697_Msal_Bot2012_COI	DEVA9	1	BC29697_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29697	Sample	BC29697	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA9	COI	45	BC29697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA9	GYTAGTTCCRATGATGTTAGCTG	146.36387
BC29699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18728	WGS84	BC29699	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29699_Aamu_Bax2006_COI	DEVA6	1	BC29699_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29699	Sample	BC29699	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA6	COI	45	BC29699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA6	GCACAACCGGGATCTTTACTTC	146.36737
BC29699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18728	WGS84	BC29699	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29699_Pcan_Bot2011_IGS	DEVA6	1	BC29699_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29699	Sample	BC29699	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA6	IGS	45	BC29699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA6	CGTAATCCTCAGTACTGGCTA	146.36737
BC29699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18728	WGS84	BC29699	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29699_Sspa_Oph2007_COI	DEVA6	1	BC29699_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29699	Sample	BC29699	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA6	COI	45	BC29699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA6	GAAGGCCTTCCGATGATC	146.36737
BC29699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18728	WGS84	BC29699	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29699_Upin_Bot2011_COI	DEVA6	1	BC29699_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29699	Sample	BC29699	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA6	COI	45	BC29699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA6	CTTTAATTACAGCGTTTTTATTGTTGT	146.36737
BC29700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1878	WGS84	BC29700	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.779	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29700_Mgig_Bot2012_COI	DEVA5	1	BC29700_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29700	Sample	BC29700	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA5	COI	45	BC29700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA5	TCTTATTCGTTGGAGACTTTATAACCCT	146.3656
BC29701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18884	WGS84	BC29701	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.876	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29701_Cmae_Bot2011_COI	DEVA4	1	BC29701_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29701	Sample	BC29701	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA4	COI	45	BC29701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA4	ATGAACAGTCTATCCTCCTTTAG	146.36796
BC29701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18884	WGS84	BC29701	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29701_Msal_Bot2012_COI	DEVA4	1	BC29701_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29701	Sample	BC29701	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA4	COI	45	BC29701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA4	GYTAGTTCCRATGATGTTAGCTG	146.36796
BC29703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.19141	WGS84	BC29703	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29703_Aamu_Bax2006_COI	DEVA1	1	BC29703_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29703	Sample	BC29703	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA1	COI	45	BC29703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA1	GCACAACCGGGATCTTTACTTC	146.36788
BC29703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.19141	WGS84	BC29703	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29703_Pcan_Bot2011_IGS	DEVA1	1	BC29703_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29703	Sample	BC29703	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA1	IGS	45	BC29703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA1	CGTAATCCTCAGTACTGGCTA	146.36788
BC29703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.19141	WGS84	BC29703	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29703_Sspa_Oph2007_COI	DEVA1	1	BC29703_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29703	Sample	BC29703	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA1	COI	45	BC29703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA1	GAAGGCCTTCCGATGATC	146.36788
BC29703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.19141	WGS84	BC29703	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29703_Upin_Bot2011_COI	DEVA1	1	BC29703_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29703	Sample	BC29703	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA1	COI	45	BC29703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA1	CTTTAATTACAGCGTTTTTATTGTTGT	146.36788
BC29704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8817	WGS84	BC29704	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.557	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29704_Mgig_Bot2012_COI	NEWA45	1	BC29704_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29704	Sample	BC29704	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA45	COI	45	BC29704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA45	TCTTATTCGTTGGAGACTTTATAACCCT	151.7493
BC29705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88231	WGS84	BC29705	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29705_Cmae_Bot2011_COI	NEWA44	1	BC29705_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29705	Sample	BC29705	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA44	COI	45	BC29705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA44	ATGAACAGTCTATCCTCCTTTAG	151.75221
BC29705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88231	WGS84	BC29705	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29705_Msal_Bot2012_COI	NEWA44	1	BC29705_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29705	Sample	BC29705	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA44	COI	45	BC29705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA44	GYTAGTTCCRATGATGTTAGCTG	151.75221
BC29707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88398	WGS84	BC29707	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29707_Aamu_Bax2006_COI	NEWA42	1	BC29707_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29707	Sample	BC29707	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA42	COI	45	BC29707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA42	GCACAACCGGGATCTTTACTTC	151.75356
BC29707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88398	WGS84	BC29707	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29707_Pcan_Bot2011_IGS	NEWA42	1	BC29707_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29707	Sample	BC29707	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA42	IGS	45	BC29707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA42	CGTAATCCTCAGTACTGGCTA	151.75356
BC29707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88398	WGS84	BC29707	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29707_Sspa_Oph2007_COI	NEWA42	1	BC29707_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29707	Sample	BC29707	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA42	COI	45	BC29707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA42	GAAGGCCTTCCGATGATC	151.75356
BC29707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88398	WGS84	BC29707	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29707_Upin_Bot2011_COI	NEWA42	1	BC29707_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29707	Sample	BC29707	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA42	COI	45	BC29707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA42	CTTTAATTACAGCGTTTTTATTGTTGT	151.75356
BC29708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88557	WGS84	BC29708	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.963	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29708_Mgig_Bot2012_COI	NEWA41	1	BC29708_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29708	Sample	BC29708	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA41	COI	45	BC29708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA41	TCTTATTCGTTGGAGACTTTATAACCCT	151.75728
BC29709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88524	WGS84	BC29709	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29709_Cmae_Bot2011_COI	NEWA40	1	BC29709_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29709	Sample	BC29709	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA40	COI	45	BC29709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA40	ATGAACAGTCTATCCTCCTTTAG	151.7552
BC29709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88524	WGS84	BC29709	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29709_Msal_Bot2012_COI	NEWA40	1	BC29709_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29709	Sample	BC29709	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA40	COI	45	BC29709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA40	GYTAGTTCCRATGATGTTAGCTG	151.7552
BC29710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88718	WGS84	BC29710	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29710_Aamu_Bax2006_COI	NEWA39	1	BC29710_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29710	Sample	BC29710	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA39	COI	45	BC29710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA39	GCACAACCGGGATCTTTACTTC	151.76167
BC29710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88718	WGS84	BC29710	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29710_Pcan_Bot2011_IGS	NEWA39	1	BC29710_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29710	Sample	BC29710	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA39	IGS	45	BC29710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA39	CGTAATCCTCAGTACTGGCTA	151.76167
BC29710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88718	WGS84	BC29710	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29710_Sspa_Oph2007_COI	NEWA39	1	BC29710_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29710	Sample	BC29710	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA39	COI	45	BC29710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA39	GAAGGCCTTCCGATGATC	151.76167
BC29710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88718	WGS84	BC29710	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29710_Upin_Bot2011_COI	NEWA39	1	BC29710_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29710	Sample	BC29710	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA39	COI	45	BC29710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA39	CTTTAATTACAGCGTTTTTATTGTTGT	151.76167
BC29711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88679	WGS84	BC29711	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29711_Mgig_Bot2012_COI	NEWA38	1	BC29711_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29711	Sample	BC29711	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWA38	COI	45	BC29711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA38	TCTTATTCGTTGGAGACTTTATAACCCT	151.75868
BC29712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88826	WGS84	BC29712	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29712_Cmae_Bot2011_COI	NEWA37	1	BC29712_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29712	Sample	BC29712	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA37	COI	45	BC29712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA37	ATGAACAGTCTATCCTCCTTTAG	151.76569
BC29712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88826	WGS84	BC29712	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29712_Msal_Bot2012_COI	NEWA37	1	BC29712_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29712	Sample	BC29712	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA37	COI	45	BC29712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA37	GYTAGTTCCRATGATGTTAGCTG	151.76569
BC29714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88836	WGS84	BC29714	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29714_Aamu_Bax2006_COI	NEWA35	1	BC29714_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29714	Sample	BC29714	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA35	COI	45	BC29714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA35	GCACAACCGGGATCTTTACTTC	151.76012
BC29714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88836	WGS84	BC29714	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29714_Pcan_Bot2011_IGS	NEWA35	1	BC29714_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29714	Sample	BC29714	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA35	IGS	45	BC29714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA35	CGTAATCCTCAGTACTGGCTA	151.76012
BC29714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88836	WGS84	BC29714	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29714_Sspa_Oph2007_COI	NEWA35	1	BC29714_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29714	Sample	BC29714	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA35	COI	45	BC29714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA35	GAAGGCCTTCCGATGATC	151.76012
BC29714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88836	WGS84	BC29714	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29714_Upin_Bot2011_COI	NEWA35	1	BC29714_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29714	Sample	BC29714	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA35	COI	45	BC29714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA35	CTTTAATTACAGCGTTTTTATTGTTGT	151.76012
BC29715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88952	WGS84	BC29715	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.729	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29715_Mgig_Bot2012_COI	NEWA34	1	BC29715_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29715	Sample	BC29715	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA34	COI	45	BC29715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA34	TCTTATTCGTTGGAGACTTTATAACCCT	151.76945
BC29716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88997	WGS84	BC29716	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29716_Cmae_Bot2011_COI	NEWA33	1	BC29716_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29716	Sample	BC29716	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA33	COI	45	BC29716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA33	ATGAACAGTCTATCCTCCTTTAG	151.7669
BC29716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88997	WGS84	BC29716	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29716_Msal_Bot2012_COI	NEWA33	1	BC29716_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29716	Sample	BC29716	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA33	COI	45	BC29716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA33	GYTAGTTCCRATGATGTTAGCTG	151.7669
BC29718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89009	WGS84	BC29718	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29718_Aamu_Bax2006_COI	NEWA31	1	BC29718_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29718	Sample	BC29718	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA31	COI	45	BC29718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA31	GCACAACCGGGATCTTTACTTC	151.77235
BC29718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89009	WGS84	BC29718	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29718_Pcan_Bot2011_IGS	NEWA31	1	BC29718_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29718	Sample	BC29718	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA31	IGS	45	BC29718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA31	CGTAATCCTCAGTACTGGCTA	151.77235
BC29718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89009	WGS84	BC29718	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29718_Sspa_Oph2007_COI	NEWA31	1	BC29718_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29718	Sample	BC29718	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA31	COI	45	BC29718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA31	GAAGGCCTTCCGATGATC	151.77235
BC29718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89009	WGS84	BC29718	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29718_Upin_Bot2011_COI	NEWA31	1	BC29718_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29718	Sample	BC29718	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA31	COI	45	BC29718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA31	CTTTAATTACAGCGTTTTTATTGTTGT	151.77235
BC29719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89109	WGS84	BC29719	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.852	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29719_Mgig_Bot2012_COI	NEWA30	1	BC29719_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29719	Sample	BC29719	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA30	COI	45	BC29719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA30	TCTTATTCGTTGGAGACTTTATAACCCT	151.77088
BC29721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89171	WGS84	BC29721	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29721_Aamu_Bax2006_COI	NEWA28	1	BC29721_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29721	Sample	BC29721	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA28	COI	45	BC29721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA28	GCACAACCGGGATCTTTACTTC	151.76605
BC29721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89171	WGS84	BC29721	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29721_Pcan_Bot2011_IGS	NEWA28	1	BC29721_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29721	Sample	BC29721	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA28	IGS	45	BC29721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA28	CGTAATCCTCAGTACTGGCTA	151.76605
BC29721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89171	WGS84	BC29721	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29721_Sspa_Oph2007_COI	NEWA28	1	BC29721_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29721	Sample	BC29721	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA28	COI	45	BC29721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA28	GAAGGCCTTCCGATGATC	151.76605
BC29721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89171	WGS84	BC29721	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29721_Upin_Bot2011_COI	NEWA28	1	BC29721_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29721	Sample	BC29721	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA28	COI	45	BC29721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA28	CTTTAATTACAGCGTTTTTATTGTTGT	151.76605
BC29722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89277	WGS84	BC29722	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.057	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29722_Mgig_Bot2012_COI	NEWA27	1	BC29722_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29722	Sample	BC29722	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA27	COI	45	BC29722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA27	TCTTATTCGTTGGAGACTTTATAACCCT	151.77197
BC29723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89279	WGS84	BC29723	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29723_Cmae_Bot2011_COI	NEWA26	1	BC29723_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29723	Sample	BC29723	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA26	COI	45	BC29723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA26	ATGAACAGTCTATCCTCCTTTAG	151.77002
BC29723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89279	WGS84	BC29723	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29723_Msal_Bot2012_COI	NEWA26	1	BC29723_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29723	Sample	BC29723	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA26	COI	45	BC29723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA26	GYTAGTTCCRATGATGTTAGCTG	151.77002
BC29725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89442	WGS84	BC29725	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29725_Aamu_Bax2006_COI	NEWA24	1	BC29725_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29725	Sample	BC29725	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA24	COI	45	BC29725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA24	GCACAACCGGGATCTTTACTTC	151.77146
BC29725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89442	WGS84	BC29725	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29725_Pcan_Bot2011_IGS	NEWA24	1	BC29725_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29725	Sample	BC29725	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA24	IGS	45	BC29725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA24	CGTAATCCTCAGTACTGGCTA	151.77146
BC29725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89442	WGS84	BC29725	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29725_Sspa_Oph2007_COI	NEWA24	1	BC29725_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29725	Sample	BC29725	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA24	COI	45	BC29725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA24	GAAGGCCTTCCGATGATC	151.77146
BC29725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89442	WGS84	BC29725	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29725_Upin_Bot2011_COI	NEWA24	1	BC29725_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29725	Sample	BC29725	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA24	COI	45	BC29725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA24	CTTTAATTACAGCGTTTTTATTGTTGT	151.77146
BC29726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89485	WGS84	BC29726	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.531	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29726_Mgig_Bot2012_COI	NEWA23	1	BC29726_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29726	Sample	BC29726	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA23	COI	45	BC29726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA23	TCTTATTCGTTGGAGACTTTATAACCCT	151.76897
BC29727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89627	WGS84	BC29727	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29727_Cmae_Bot2011_COI	NEWA22	1	BC29727_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29727	Sample	BC29727	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA22	COI	45	BC29727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA22	ATGAACAGTCTATCCTCCTTTAG	151.77242
BC29727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89627	WGS84	BC29727	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29727_Msal_Bot2012_COI	NEWA22	1	BC29727_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29727	Sample	BC29727	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA22	COI	45	BC29727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA22	GYTAGTTCCRATGATGTTAGCTG	151.77242
BC29729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91159	WGS84	BC29729	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29729_Aamu_Bax2006_COI	NEWA20	1	BC29729_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29729	Sample	BC29729	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA20	COI	45	BC29729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA20	GCACAACCGGGATCTTTACTTC	151.77401
BC29729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91159	WGS84	BC29729	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29729_Pcan_Bot2011_IGS	NEWA20	1	BC29729_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29729	Sample	BC29729	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA20	IGS	45	BC29729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA20	CGTAATCCTCAGTACTGGCTA	151.77401
BC29729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91159	WGS84	BC29729	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29729_Sspa_Oph2007_COI	NEWA20	1	BC29729_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29729	Sample	BC29729	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA20	COI	45	BC29729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA20	GAAGGCCTTCCGATGATC	151.77401
BC29729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91159	WGS84	BC29729	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29729_Upin_Bot2011_COI	NEWA20	1	BC29729_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29729	Sample	BC29729	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA20	COI	45	BC29729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA20	CTTTAATTACAGCGTTTTTATTGTTGT	151.77401
BC29730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91424	WGS84	BC29730	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29730_Cmae_Bot2011_COI	NEWA19	1	BC29730_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29730	Sample	BC29730	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA19	COI	45	BC29730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA19	ATGAACAGTCTATCCTCCTTTAG	151.77313
BC29730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91424	WGS84	BC29730	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29730_Msal_Bot2012_COI	NEWA19	1	BC29730_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29730	Sample	BC29730	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA19	COI	45	BC29730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA19	GYTAGTTCCRATGATGTTAGCTG	151.77313
BC29732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91678	WGS84	BC29732	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29732_Aamu_Bax2006_COI	NEWA17	1	BC29732_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29732	Sample	BC29732	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA17	COI	45	BC29732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA17	GCACAACCGGGATCTTTACTTC	151.77668
BC29732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91678	WGS84	BC29732	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29732_Pcan_Bot2011_IGS	NEWA17	1	BC29732_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29732	Sample	BC29732	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA17	IGS	45	BC29732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA17	CGTAATCCTCAGTACTGGCTA	151.77668
BC29732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91678	WGS84	BC29732	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29732_Sspa_Oph2007_COI	NEWA17	1	BC29732_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29732	Sample	BC29732	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA17	COI	45	BC29732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA17	GAAGGCCTTCCGATGATC	151.77668
BC29732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91678	WGS84	BC29732	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29732_Upin_Bot2011_COI	NEWA17	1	BC29732_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29732	Sample	BC29732	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA17	COI	45	BC29732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA17	CTTTAATTACAGCGTTTTTATTGTTGT	151.77668
BC29733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91741	WGS84	BC29733	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.876	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29733_Mgig_Bot2012_COI	NEWA16	1	BC29733_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29733	Sample	BC29733	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA16	COI	45	BC29733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA16	TCTTATTCGTTGGAGACTTTATAACCCT	151.774
BC29734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91799	WGS84	BC29734	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29734_Cmae_Bot2011_COI	NEWA15	1	BC29734_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29734	Sample	BC29734	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA15	COI	45	BC29734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA15	ATGAACAGTCTATCCTCCTTTAG	151.77576
BC29734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91799	WGS84	BC29734	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29734_Msal_Bot2012_COI	NEWA15	1	BC29734_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29734	Sample	BC29734	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA15	COI	45	BC29734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA15	GYTAGTTCCRATGATGTTAGCTG	151.77576
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29736_Aamu_Bax2006_COI	NEWA13	1	BC29736_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	GCACAACCGGGATCTTTACTTC	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29736_Pcan_Bot2011_IGS	NEWA13	1	BC29736_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA13	IGS	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	CGTAATCCTCAGTACTGGCTA	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29736_Pper_Dia2013_COI	NEWA13	1	BC29736_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	CTTAGTGGCATTAATTCGDAATCC	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29736_Sspa_Oph2007_COI	NEWA13	1	BC29736_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	GAAGGCCTTCCGATGATC	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29736_Upin_Bot2011_COI	NEWA13	1	BC29736_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	CTTTAATTACAGCGTTTTTATTGTTGT	151.77052
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29737_Cjap_Sim2018_COI	NEWA12	1	BC29737_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29737_Esin_And2018_Cyb	NEWA12	1	BC29737_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA12	Cytb	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	ACCCCTCCTCATATCCAACCA	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.116	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29737_Mgig_Bot2012_COI	NEWA12	1	BC29737_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	TCTTATTCGTTGGAGACTTTATAACCCT	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29737_Mjap_Gib2022_COI	NEWA12	1	BC29737_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	AATTTAATTGTGACTAGGCATGGTCTG	151.77158
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29738_Cmae_Bot2011_COI	NEWA11	1	BC29738_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	ATGAACAGTCTATCCTCCTTTAG	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29738_Hsan_Wil2021_COI	NEWA11	1	BC29738_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	CCTGGGCCGGTATAGTAGGT	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29738_Mros_Gib2022_COI	NEWA11	1	BC29738_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29738_Msal_Bot2012_COI	NEWA11	1	BC29738_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	GYTAGTTCCRATGATGTTAGCTG	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29738_Pvir_Dia2013_COI	NEWA11	1	BC29738_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	CTTAGTGGCATTAATTCGDAATCC	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29738_Rhar_Sim2018_COI	NEWA11	1	BC29738_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	CTGCTGCTATTGCTCATG	151.77323
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29740_Cjap_Sim2018_COI	NEWA9	1	BC29740_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29740_Esin_And2018_Cyb	NEWA9	1	BC29740_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA9	Cytb	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	ACCCCTCCTCATATCCAACCA	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.094	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29740_Mgig_Bot2012_COI	NEWA9	1	BC29740_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	TCTTATTCGTTGGAGACTTTATAACCCT	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29740_Mjap_Gib2022_COI	NEWA9	1	BC29740_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	AATTTAATTGTGACTAGGCATGGTCTG	151.7724
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29741_Cmae_Bot2011_COI	NEWA8	1	BC29741_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	ATGAACAGTCTATCCTCCTTTAG	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29741_Hsan_Wil2021_COI	NEWA8	1	BC29741_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	CCTGGGCCGGTATAGTAGGT	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29741_Mros_Gib2022_COI	NEWA8	1	BC29741_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29741_Msal_Bot2012_COI	NEWA8	1	BC29741_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	GYTAGTTCCRATGATGTTAGCTG	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29741_Pvir_Dia2013_COI	NEWA8	1	BC29741_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	CTTAGTGGCATTAATTCGDAATCC	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29741_Rhar_Sim2018_COI	NEWA8	1	BC29741_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	CTGCTGCTATTGCTCATG	151.77154
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29743_Aamu_Bax2006_COI	NEWA6	1	BC29743_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	GCACAACCGGGATCTTTACTTC	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29743_Pcan_Bot2011_IGS	NEWA6	1	BC29743_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA6	IGS	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	CGTAATCCTCAGTACTGGCTA	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29743_Pper_Dia2013_COI	NEWA6	1	BC29743_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	CTTAGTGGCATTAATTCGDAATCC	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29743_Sspa_Oph2007_COI	NEWA6	1	BC29743_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	GAAGGCCTTCCGATGATC	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29743_Upin_Bot2011_COI	NEWA6	1	BC29743_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	CTTTAATTACAGCGTTTTTATTGTTGT	151.77058
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29744_Cjap_Sim2018_COI	NEWA5	1	BC29744_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29744_Esin_And2018_Cyb	NEWA5	1	BC29744_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA5	Cytb	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	ACCCCTCCTCATATCCAACCA	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.61	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29744_Mgig_Bot2012_COI	NEWA5	1	BC29744_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	TCTTATTCGTTGGAGACTTTATAACCCT	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29744_Mjap_Gib2022_COI	NEWA5	1	BC29744_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	AATTTAATTGTGACTAGGCATGGTCTG	151.76814
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29745_Cmae_Bot2011_COI	NEWA4	1	BC29745_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	ATGAACAGTCTATCCTCCTTTAG	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29745_Hsan_Wil2021_COI	NEWA4	1	BC29745_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	CCTGGGCCGGTATAGTAGGT	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29745_Mros_Gib2022_COI	NEWA4	1	BC29745_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29745_Msal_Bot2012_COI	NEWA4	1	BC29745_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	GYTAGTTCCRATGATGTTAGCTG	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29745_Pvir_Dia2013_COI	NEWA4	1	BC29745_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	CTTAGTGGCATTAATTCGDAATCC	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29745_Rhar_Sim2018_COI	NEWA4	1	BC29745_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	CTGCTGCTATTGCTCATG	151.77353
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29747_Aamu_Bax2006_COI	NEWA2	1	BC29747_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	GCACAACCGGGATCTTTACTTC	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29747_Pcan_Bot2011_IGS	NEWA2	1	BC29747_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA2	IGS	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	CGTAATCCTCAGTACTGGCTA	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29747_Pper_Dia2013_COI	NEWA2	1	BC29747_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	CTTAGTGGCATTAATTCGDAATCC	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29747_Sspa_Oph2007_COI	NEWA2	1	BC29747_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	GAAGGCCTTCCGATGATC	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29747_Upin_Bot2011_COI	NEWA2	1	BC29747_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	CTTTAATTACAGCGTTTTTATTGTTGT	151.76782
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29748_Cjap_Sim2018_COI	NEWA1	1	BC29748_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29748_Esin_And2018_Cyb	NEWA1	1	BC29748_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA1	Cytb	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	ACCCCTCCTCATATCCAACCA	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.813	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29748_Mgig_Bot2012_COI	NEWA1	1	BC29748_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	TCTTATTCGTTGGAGACTTTATAACCCT	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29748_Mjap_Gib2022_COI	NEWA1	1	BC29748_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	AATTTAATTGTGACTAGGCATGGTCTG	151.76515
BC29749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4533	WGS84	BC29749	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29749_Cmae_Bot2011_COI	KEMA35	1	BC29749_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29749	Sample	BC29749	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA35	COI	45	BC29749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA35	ATGAACAGTCTATCCTCCTTTAG	150.8953
BC29749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4533	WGS84	BC29749	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29749_Msal_Bot2012_COI	KEMA35	1	BC29749_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29749	Sample	BC29749	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA35	COI	45	BC29749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA35	GYTAGTTCCRATGATGTTAGCTG	150.8953
BC29750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45408	WGS84	BC29750	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29750_Aamu_Bax2006_COI	KEMA34	1	BC29750_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29750	Sample	BC29750	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA34	COI	45	BC29750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA34	GCACAACCGGGATCTTTACTTC	150.89427
BC29750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45408	WGS84	BC29750	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29750_Pcan_Bot2011_IGS	KEMA34	1	BC29750_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29750	Sample	BC29750	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA34	IGS	45	BC29750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA34	CGTAATCCTCAGTACTGGCTA	150.89427
BC29750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45408	WGS84	BC29750	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29750_Sspa_Oph2007_COI	KEMA34	1	BC29750_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29750	Sample	BC29750	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA34	COI	45	BC29750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA34	GAAGGCCTTCCGATGATC	150.89427
BC29750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45408	WGS84	BC29750	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29750_Upin_Bot2011_COI	KEMA34	1	BC29750_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29750	Sample	BC29750	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA34	COI	45	BC29750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA34	CTTTAATTACAGCGTTTTTATTGTTGT	150.89427
BC29751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45615	WGS84	BC29751	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.639	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29751_Mgig_Bot2012_COI	KEMA33	1	BC29751_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29751	Sample	BC29751	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA33	COI	45	BC29751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA33	TCTTATTCGTTGGAGACTTTATAACCCT	150.88729
BC29752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45566	WGS84	BC29752	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29752_Cmae_Bot2011_COI	KEMA32	1	BC29752_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29752	Sample	BC29752	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA32	COI	45	BC29752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA32	ATGAACAGTCTATCCTCCTTTAG	150.89579
BC29752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45566	WGS84	BC29752	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29752_Msal_Bot2012_COI	KEMA32	1	BC29752_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29752	Sample	BC29752	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA32	COI	45	BC29752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA32	GYTAGTTCCRATGATGTTAGCTG	150.89579
BC29754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45643	WGS84	BC29754	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29754_Aamu_Bax2006_COI	KEMA30	1	BC29754_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29754	Sample	BC29754	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA30	COI	45	BC29754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA30	GCACAACCGGGATCTTTACTTC	150.88892
BC29754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45643	WGS84	BC29754	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29754_Pcan_Bot2011_IGS	KEMA30	1	BC29754_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29754	Sample	BC29754	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA30	IGS	45	BC29754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA30	CGTAATCCTCAGTACTGGCTA	150.88892
BC29754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45643	WGS84	BC29754	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29754_Sspa_Oph2007_COI	KEMA30	1	BC29754_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29754	Sample	BC29754	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA30	COI	45	BC29754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA30	GAAGGCCTTCCGATGATC	150.88892
BC29754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45643	WGS84	BC29754	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29754_Upin_Bot2011_COI	KEMA30	1	BC29754_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29754	Sample	BC29754	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA30	COI	45	BC29754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA30	CTTTAATTACAGCGTTTTTATTGTTGT	150.88892
BC29755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45745	WGS84	BC29755	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.502	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29755_Mgig_Bot2012_COI	KEMA29	1	BC29755_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29755	Sample	BC29755	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA29	COI	45	BC29755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA29	TCTTATTCGTTGGAGACTTTATAACCCT	150.89471
BC29756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45774	WGS84	BC29756	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29756_Cmae_Bot2011_COI	KEMA28	1	BC29756_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29756	Sample	BC29756	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA28	COI	45	BC29756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA28	ATGAACAGTCTATCCTCCTTTAG	150.89314
BC29756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45774	WGS84	BC29756	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29756_Msal_Bot2012_COI	KEMA28	1	BC29756_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29756	Sample	BC29756	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA28	COI	45	BC29756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA28	GYTAGTTCCRATGATGTTAGCTG	150.89314
BC29758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45976	WGS84	BC29758	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29758_Aamu_Bax2006_COI	KEMA26	1	BC29758_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29758	Sample	BC29758	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA26	COI	45	BC29758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA26	GCACAACCGGGATCTTTACTTC	150.89679
BC29758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45976	WGS84	BC29758	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29758_Pcan_Bot2011_IGS	KEMA26	1	BC29758_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29758	Sample	BC29758	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA26	IGS	45	BC29758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA26	CGTAATCCTCAGTACTGGCTA	150.89679
BC29758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45976	WGS84	BC29758	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29758_Sspa_Oph2007_COI	KEMA26	1	BC29758_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29758	Sample	BC29758	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA26	COI	45	BC29758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA26	GAAGGCCTTCCGATGATC	150.89679
BC29758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45976	WGS84	BC29758	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29758_Upin_Bot2011_COI	KEMA26	1	BC29758_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29758	Sample	BC29758	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA26	COI	45	BC29758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA26	CTTTAATTACAGCGTTTTTATTGTTGT	150.89679
BC29759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45991	WGS84	BC29759	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.226	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29759_Mgig_Bot2012_COI	KEMA25	1	BC29759_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29759	Sample	BC29759	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA25	COI	45	BC29759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA25	TCTTATTCGTTGGAGACTTTATAACCCT	150.89441
BC29761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4613	WGS84	BC29761	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29761_Aamu_Bax2006_COI	KEMA23	1	BC29761_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29761	Sample	BC29761	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA23	COI	45	BC29761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA23	GCACAACCGGGATCTTTACTTC	150.89796
BC29761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4613	WGS84	BC29761	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29761_Pcan_Bot2011_IGS	KEMA23	1	BC29761_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29761	Sample	BC29761	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA23	IGS	45	BC29761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA23	CGTAATCCTCAGTACTGGCTA	150.89796
BC29761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4613	WGS84	BC29761	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29761_Sspa_Oph2007_COI	KEMA23	1	BC29761_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29761	Sample	BC29761	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA23	COI	45	BC29761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA23	GAAGGCCTTCCGATGATC	150.89796
BC29761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4613	WGS84	BC29761	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29761_Upin_Bot2011_COI	KEMA23	1	BC29761_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29761	Sample	BC29761	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA23	COI	45	BC29761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA23	CTTTAATTACAGCGTTTTTATTGTTGT	150.89796
BC29762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46148	WGS84	BC29762	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.479	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29762_Mgig_Bot2012_COI	KEMA22	1	BC29762_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29762	Sample	BC29762	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA22	COI	45	BC29762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA22	TCTTATTCGTTGGAGACTTTATAACCCT	150.89598
BC29763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46143	WGS84	BC29763	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29763_Cmae_Bot2011_COI	KEMA21	1	BC29763_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29763	Sample	BC29763	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA21	COI	45	BC29763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA21	ATGAACAGTCTATCCTCCTTTAG	150.89383
BC29763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46143	WGS84	BC29763	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29763_Msal_Bot2012_COI	KEMA21	1	BC29763_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29763	Sample	BC29763	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA21	COI	45	BC29763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA21	GYTAGTTCCRATGATGTTAGCTG	150.89383
BC29765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46331	WGS84	BC29765	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29765_Aamu_Bax2006_COI	KEMA19	1	BC29765_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29765	Sample	BC29765	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA19	COI	45	BC29765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA19	GCACAACCGGGATCTTTACTTC	150.90459
BC29765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46331	WGS84	BC29765	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29765_Pcan_Bot2011_IGS	KEMA19	1	BC29765_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29765	Sample	BC29765	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA19	IGS	45	BC29765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA19	CGTAATCCTCAGTACTGGCTA	150.90459
BC29765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46331	WGS84	BC29765	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29765_Upin_Bot2011_COI	KEMA19	1	BC29765_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29765	Sample	BC29765	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA19	COI	45	BC29765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA19	CTTTAATTACAGCGTTTTTATTGTTGT	150.90459
BC29766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46396	WGS84	BC29766	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.928	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29766_Mgig_Bot2012_COI	KEMA18	1	BC29766_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29766	Sample	BC29766	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA18	COI	45	BC29766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA18	TCTTATTCGTTGGAGACTTTATAACCCT	150.90323
BC29767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46402	WGS84	BC29767	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29767_Cmae_Bot2011_COI	KEMA17	1	BC29767_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29767	Sample	BC29767	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA17	COI	45	BC29767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA17	ATGAACAGTCTATCCTCCTTTAG	150.90113
BC29767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46402	WGS84	BC29767	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29767_Msal_Bot2012_COI	KEMA17	1	BC29767_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29767	Sample	BC29767	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA17	COI	45	BC29767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA17	GYTAGTTCCRATGATGTTAGCTG	150.90113
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46501	WGS84	BC29769	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29769_Aamu_Bax2006_COI	KEMA15	1	BC29769_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA15	COI	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	GCACAACCGGGATCTTTACTTC	150.90702
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46501	WGS84	BC29769	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29769_Pcan_Bot2011_IGS	KEMA15	1	BC29769_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA15	IGS	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	CGTAATCCTCAGTACTGGCTA	150.90702
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46501	WGS84	BC29769	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29769_Sspa_Oph2007_COI	KEMA15	1	BC29769_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA15	COI	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	GAAGGCCTTCCGATGATC	150.90702
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46501	WGS84	BC29769	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29769_Vgib_Wil2025_COI	KEMA15	1	BC29769_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA15	COI	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	CCCCTCTTTACAAGTTCTTTAGGGT	150.90702
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46527	WGS84	BC29770	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29770_Cmae_Bot2011_COI	KEMA13	1	BC29770_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	ATGAACAGTCTATCCTCCTTTAG	150.90138
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46527	WGS84	BC29770	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29770_Msal_Bot2012_COI	KEMA13	1	BC29770_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	GYTAGTTCCRATGATGTTAGCTG	150.90138
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46705	WGS84	BC29772	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29772_Aamu_Bax2006_COI	KEMA11	1	BC29772_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	GCACAACCGGGATCTTTACTTC	150.90599
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46705	WGS84	BC29772	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29772_Pcan_Bot2011_IGS	KEMA11	1	BC29772_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA11	IGS	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	CGTAATCCTCAGTACTGGCTA	150.90599
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46705	WGS84	BC29772	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29772_Sspa_Oph2007_COI	KEMA11	1	BC29772_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	GAAGGCCTTCCGATGATC	150.90599
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46705	WGS84	BC29772	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29772_Upin_Bot2011_COI	KEMA11	1	BC29772_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	CTTTAATTACAGCGTTTTTATTGTTGT	150.90599
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46705	WGS84	BC29772	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29772_Vgib_Wil2025_COI	KEMA11	1	BC29772_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	CCCCTCTTTACAAGTTCTTTAGGGT	150.90599
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46723	WGS84	BC29773	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29773_Asen_Wil2025_COI	KEMA10	1	BC29773_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	GTTGTTGTTACAACACATGCTCTTTTG	150.90388
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46723	WGS84	BC29773	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.628	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29773_Mgig_Bot2012_COI	KEMA10	1	BC29773_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	TCTTATTCGTTGGAGACTTTATAACCCT	150.90388
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46537	WGS84	BC29774	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29774_Cmae_Bot2011_COI	KEMA14	1	BC29774_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	ATGAACAGTCTATCCTCCTTTAG	150.90488
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46537	WGS84	BC29774	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29774_Msal_Bot2012_COI	KEMA14	1	BC29774_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	GYTAGTTCCRATGATGTTAGCTG	150.90488
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46841	WGS84	BC29776	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29776_Aamu_Bax2006_COI	KEMA8	1	BC29776_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	GCACAACCGGGATCTTTACTTC	150.90758
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46841	WGS84	BC29776	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29776_Pcan_Bot2011_IGS	KEMA8	1	BC29776_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA8	IGS	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	CGTAATCCTCAGTACTGGCTA	150.90758
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46841	WGS84	BC29776	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29776_Sspa_Oph2007_COI	KEMA8	1	BC29776_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	GAAGGCCTTCCGATGATC	150.90758
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46841	WGS84	BC29776	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29776_Upin_Bot2011_COI	KEMA8	1	BC29776_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	CTTTAATTACAGCGTTTTTATTGTTGT	150.90758
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46841	WGS84	BC29776	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29776_Vgib_Wil2025_COI	KEMA8	1	BC29776_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	CCCCTCTTTACAAGTTCTTTAGGGT	150.90758
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46844	WGS84	BC29777	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29777_Asen_Wil2025_COI	KEMA7	1	BC29777_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	GTTGTTGTTACAACACATGCTCTTTTG	150.90496
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46844	WGS84	BC29777	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.612	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29777_Mgig_Bot2012_COI	KEMA7	1	BC29777_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	TCTTATTCGTTGGAGACTTTATAACCCT	150.90496
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4701	WGS84	BC29778	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29778_Cmae_Bot2011_COI	KEMA6	1	BC29778_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	ATGAACAGTCTATCCTCCTTTAG	150.9063
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4701	WGS84	BC29778	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29778_Msal_Bot2012_COI	KEMA6	1	BC29778_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	GYTAGTTCCRATGATGTTAGCTG	150.9063
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.4697	WGS84	BC29780	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29780_Asen_Wil2025_COI	KEMA4	1	BC29780_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	GTTGTTGTTACAACACATGCTCTTTTG	150.90884
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4697	WGS84	BC29780	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.367	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29780_Mgig_Bot2012_COI	KEMA4	1	BC29780_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	TCTTATTCGTTGGAGACTTTATAACCCT	150.90884
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47125	WGS84	BC29781	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29781_Cmae_Bot2011_COI	KEMA3	1	BC29781_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	ATGAACAGTCTATCCTCCTTTAG	150.91025
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47125	WGS84	BC29781	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29781_Msal_Bot2012_COI	KEMA3	1	BC29781_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	GYTAGTTCCRATGATGTTAGCTG	150.91025
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47312	WGS84	BC29783	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29783_Aamu_Bax2006_COI	KEMA1	1	BC29783_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	GCACAACCGGGATCTTTACTTC	150.90939
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47312	WGS84	BC29783	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29783_Pcan_Bot2011_IGS	KEMA1	1	BC29783_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA1	IGS	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	CGTAATCCTCAGTACTGGCTA	150.90939
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47312	WGS84	BC29783	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29783_Sspa_Oph2007_COI	KEMA1	1	BC29783_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	GAAGGCCTTCCGATGATC	150.90939
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47312	WGS84	BC29783	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29783_Upin_Bot2011_COI	KEMA1	1	BC29783_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	CTTTAATTACAGCGTTTTTATTGTTGT	150.90939
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.47312	WGS84	BC29783	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29783_Vgib_Wil2025_COI	KEMA1	1	BC29783_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	CCCCTCTTTACAAGTTCTTTAGGGT	150.90939
BC29784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96258	WGS84	BC29784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.416	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29784_Mgig_Bot2012_COI	BOTA40	1	BC29784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29784	Sample	BC29784	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA40	COI	45	BC29784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA40	TCTTATTCGTTGGAGACTTTATAACCCT	151.19841
BC29785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.95907	WGS84	BC29785	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29785_Cmae_Bot2011_COI	BOTA42	1	BC29785_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29785	Sample	BC29785	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA42	COI	45	BC29785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA42	ATGAACAGTCTATCCTCCTTTAG	151.1975
BC29785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.95907	WGS84	BC29785	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29785_Msal_Bot2012_COI	BOTA42	1	BC29785_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29785	Sample	BC29785	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA42	COI	45	BC29785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA42	GYTAGTTCCRATGATGTTAGCTG	151.1975
BC29787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96424	WGS84	BC29787	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29787_Aamu_Bax2006_COI	BOTA39	1	BC29787_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29787	Sample	BC29787	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA39	COI	45	BC29787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA39	GCACAACCGGGATCTTTACTTC	151.19718
BC29787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96424	WGS84	BC29787	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29787_Pcan_Bot2011_IGS	BOTA39	1	BC29787_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29787	Sample	BC29787	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA39	IGS	45	BC29787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA39	CGTAATCCTCAGTACTGGCTA	151.19718
BC29787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96424	WGS84	BC29787	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29787_Sspa_Oph2007_COI	BOTA39	1	BC29787_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29787	Sample	BC29787	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA39	COI	45	BC29787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA39	GAAGGCCTTCCGATGATC	151.19718
BC29787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96424	WGS84	BC29787	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29787_Upin_Bot2011_COI	BOTA39	1	BC29787_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29787	Sample	BC29787	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA39	COI	45	BC29787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA39	CTTTAATTACAGCGTTTTTATTGTTGT	151.19718
BC29788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96557	WGS84	BC29788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.435	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29788_Mgig_Bot2012_COI	BOTA38	1	BC29788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29788	Sample	BC29788	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA38	COI	45	BC29788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA38	TCTTATTCGTTGGAGACTTTATAACCCT	151.19871
BC29789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96755	WGS84	BC29789	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29789_Cmae_Bot2011_COI	BOTA37	1	BC29789_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29789	Sample	BC29789	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA37	COI	45	BC29789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA37	ATGAACAGTCTATCCTCCTTTAG	151.19821
BC29789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96755	WGS84	BC29789	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29789_Msal_Bot2012_COI	BOTA37	1	BC29789_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29789	Sample	BC29789	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA37	COI	45	BC29789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA37	GYTAGTTCCRATGATGTTAGCTG	151.19821
BC29790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96914	WGS84	BC29790	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29790_Aamu_Bax2006_COI	BOTA36	1	BC29790_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29790	Sample	BC29790	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA36	COI	45	BC29790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA36	GCACAACCGGGATCTTTACTTC	151.19959
BC29790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96914	WGS84	BC29790	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29790_Pcan_Bot2011_IGS	BOTA36	1	BC29790_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29790	Sample	BC29790	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA36	IGS	45	BC29790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA36	CGTAATCCTCAGTACTGGCTA	151.19959
BC29790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96914	WGS84	BC29790	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29790_Sspa_Oph2007_COI	BOTA36	1	BC29790_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29790	Sample	BC29790	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA36	COI	45	BC29790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA36	GAAGGCCTTCCGATGATC	151.19959
BC29790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96914	WGS84	BC29790	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29790_Upin_Bot2011_COI	BOTA36	1	BC29790_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29790	Sample	BC29790	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA36	COI	45	BC29790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA36	CTTTAATTACAGCGTTTTTATTGTTGT	151.19959
BC29791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9685	WGS84	BC29791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.754	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29791_Mgig_Bot2012_COI	BOTA35	1	BC29791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29791	Sample	BC29791	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA35	COI	45	BC29791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA35	TCTTATTCGTTGGAGACTTTATAACCCT	151.2182
BC29792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96878	WGS84	BC29792	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29792_Cmae_Bot2011_COI	BOTA34	1	BC29792_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29792	Sample	BC29792	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA34	COI	45	BC29792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA34	ATGAACAGTCTATCCTCCTTTAG	151.21625
BC29792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96878	WGS84	BC29792	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29792_Msal_Bot2012_COI	BOTA34	1	BC29792_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29792	Sample	BC29792	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA34	COI	45	BC29792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA34	GYTAGTTCCRATGATGTTAGCTG	151.21625
BC29794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97029	WGS84	BC29794	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29794_Aamu_Bax2006_COI	BOTA32	1	BC29794_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29794	Sample	BC29794	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA32	COI	45	BC29794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA32	GCACAACCGGGATCTTTACTTC	151.20081
BC29794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97029	WGS84	BC29794	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29794_Pcan_Bot2011_IGS	BOTA32	1	BC29794_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29794	Sample	BC29794	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA32	IGS	45	BC29794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA32	CGTAATCCTCAGTACTGGCTA	151.20081
BC29794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97029	WGS84	BC29794	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.511	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29794_Sspa_Oph2007_COI	BOTA32	1	BC29794_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29794	Sample	BC29794	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA32	COI	45	BC29794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA32	GAAGGCCTTCCGATGATC	151.20081
BC29794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97029	WGS84	BC29794	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29794_Upin_Bot2011_COI	BOTA32	1	BC29794_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29794	Sample	BC29794	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA32	COI	45	BC29794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA32	CTTTAATTACAGCGTTTTTATTGTTGT	151.20081
BC29795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97095	WGS84	BC29795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.92	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29795_Mgig_Bot2012_COI	BOTA31	1	BC29795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29795	Sample	BC29795	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA31	COI	45	BC29795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA31	TCTTATTCGTTGGAGACTTTATAACCCT	151.19958
BC29796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97016	WGS84	BC29796	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29796_Cmae_Bot2011_COI	BOTA30	1	BC29796_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29796	Sample	BC29796	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA30	COI	45	BC29796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA30	ATGAACAGTCTATCCTCCTTTAG	151.21513
BC29796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97016	WGS84	BC29796	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29796_Msal_Bot2012_COI	BOTA30	1	BC29796_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29796	Sample	BC29796	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA30	COI	45	BC29796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA30	GYTAGTTCCRATGATGTTAGCTG	151.21513
BC29798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97089	WGS84	BC29798	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29798_Aamu_Bax2006_COI	BOTA28	1	BC29798_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29798	Sample	BC29798	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA28	COI	45	BC29798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA28	GCACAACCGGGATCTTTACTTC	151.21098
BC29798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97089	WGS84	BC29798	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29798_Pcan_Bot2011_IGS	BOTA28	1	BC29798_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29798	Sample	BC29798	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA28	IGS	45	BC29798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA28	CGTAATCCTCAGTACTGGCTA	151.21098
BC29798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97089	WGS84	BC29798	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.891	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29798_Sspa_Oph2007_COI	BOTA28	1	BC29798_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29798	Sample	BC29798	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA28	COI	45	BC29798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA28	GAAGGCCTTCCGATGATC	151.21098
BC29798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97089	WGS84	BC29798	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29798_Upin_Bot2011_COI	BOTA28	1	BC29798_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29798	Sample	BC29798	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA28	COI	45	BC29798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA28	CTTTAATTACAGCGTTTTTATTGTTGT	151.21098
BC29799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9711	WGS84	BC29799	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.283	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29799_Mgig_Bot2012_COI	BOTA27	1	BC29799_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29799	Sample	BC29799	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA27	COI	45	BC29799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA27	TCTTATTCGTTGGAGACTTTATAACCCT	151.20951
BC29800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97167	WGS84	BC29800	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29800_Cmae_Bot2011_COI	BOTA26	1	BC29800_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29800	Sample	BC29800	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA26	COI	45	BC29800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA26	ATGAACAGTCTATCCTCCTTTAG	151.20769
BC29800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97167	WGS84	BC29800	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29800_Msal_Bot2012_COI	BOTA26	1	BC29800_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29800	Sample	BC29800	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA26	COI	45	BC29800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA26	GYTAGTTCCRATGATGTTAGCTG	151.20769
BC29802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97198	WGS84	BC29802	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29802_Aamu_Bax2006_COI	BOTA24	1	BC29802_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29802	Sample	BC29802	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA24	COI	45	BC29802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA24	GCACAACCGGGATCTTTACTTC	151.2028
BC29802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97198	WGS84	BC29802	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29802_Pcan_Bot2011_IGS	BOTA24	1	BC29802_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29802	Sample	BC29802	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA24	IGS	45	BC29802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA24	CGTAATCCTCAGTACTGGCTA	151.2028
BC29802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97198	WGS84	BC29802	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29802_Sspa_Oph2007_COI	BOTA24	1	BC29802_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29802	Sample	BC29802	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA24	COI	45	BC29802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA24	GAAGGCCTTCCGATGATC	151.2028
BC29802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97198	WGS84	BC29802	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29802_Upin_Bot2011_COI	BOTA24	1	BC29802_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29802	Sample	BC29802	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA24	COI	45	BC29802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA24	CTTTAATTACAGCGTTTTTATTGTTGT	151.2028
BC29803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97218	WGS84	BC29803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.364	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29803_Mgig_Bot2012_COI	BOTA23	1	BC29803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29803	Sample	BC29803	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA23	COI	45	BC29803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA23	TCTTATTCGTTGGAGACTTTATAACCCT	151.20066
BC29804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9725	WGS84	BC29804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29804_Cmae_Bot2011_COI	BOTA22	1	BC29804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29804	Sample	BC29804	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA22	COI	45	BC29804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA22	ATGAACAGTCTATCCTCCTTTAG	151.21045
BC29804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9725	WGS84	BC29804	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29804_Msal_Bot2012_COI	BOTA22	1	BC29804_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29804	Sample	BC29804	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA22	COI	45	BC29804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA22	GYTAGTTCCRATGATGTTAGCTG	151.21045
BC29806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97333	WGS84	BC29806	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29806_Aamu_Bax2006_COI	BOTA20	1	BC29806_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29806	Sample	BC29806	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA20	COI	45	BC29806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA20	GCACAACCGGGATCTTTACTTC	151.20691
BC29806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97333	WGS84	BC29806	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29806_Pcan_Bot2011_IGS	BOTA20	1	BC29806_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29806	Sample	BC29806	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA20	IGS	45	BC29806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA20	CGTAATCCTCAGTACTGGCTA	151.20691
BC29806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97333	WGS84	BC29806	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.854	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29806_Sspa_Oph2007_COI	BOTA20	1	BC29806_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29806	Sample	BC29806	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA20	COI	45	BC29806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA20	GAAGGCCTTCCGATGATC	151.20691
BC29806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97333	WGS84	BC29806	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29806_Upin_Bot2011_COI	BOTA20	1	BC29806_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29806	Sample	BC29806	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA20	COI	45	BC29806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA20	CTTTAATTACAGCGTTTTTATTGTTGT	151.20691
BC29807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9734	WGS84	BC29807	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.355	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29807_Mgig_Bot2012_COI	BOTA19	1	BC29807_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29807	Sample	BC29807	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA19	COI	45	BC29807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA19	TCTTATTCGTTGGAGACTTTATAACCCT	151.20403
BC29808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97356	WGS84	BC29808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29808_Cmae_Bot2011_COI	BOTA18	1	BC29808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29808	Sample	BC29808	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA18	COI	45	BC29808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA18	ATGAACAGTCTATCCTCCTTTAG	151.2016
BC29808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97356	WGS84	BC29808	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29808_Msal_Bot2012_COI	BOTA18	1	BC29808_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29808	Sample	BC29808	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA18	COI	45	BC29808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA18	GYTAGTTCCRATGATGTTAGCTG	151.2016
BC29810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.974	WGS84	BC29810	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.931	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29810_Mgig_Bot2012_COI	BOTA16	1	BC29810_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29810	Sample	BC29810	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA16	COI	45	BC29810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA16	TCTTATTCGTTGGAGACTTTATAACCCT	151.2075
BC29811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97542	WGS84	BC29811	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29811_Cmae_Bot2011_COI	BOTA15	1	BC29811_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29811	Sample	BC29811	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA15	COI	45	BC29811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA15	ATGAACAGTCTATCCTCCTTTAG	151.20835
BC29811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97542	WGS84	BC29811	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29811_Msal_Bot2012_COI	BOTA15	1	BC29811_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29811	Sample	BC29811	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA15	COI	45	BC29811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA15	GYTAGTTCCRATGATGTTAGCTG	151.20835
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29813_Aamu_Bax2006_COI	BOTA13	1	BC29813_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	GCACAACCGGGATCTTTACTTC	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29813_Pcan_Bot2011_IGS	BOTA13	1	BC29813_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA13	IGS	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	CGTAATCCTCAGTACTGGCTA	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29813_Pper_Dia2013_COI	BOTA13	1	BC29813_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	CTTAGTGGCATTAATTCGDAATCC	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29813_Sspa_Oph2007_COI	BOTA13	1	BC29813_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	GAAGGCCTTCCGATGATC	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29813_Upin_Bot2011_COI	BOTA13	1	BC29813_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	CTTTAATTACAGCGTTTTTATTGTTGT	151.20882
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29814_Cjap_Sim2018_COI	BOTA12	1	BC29814_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29814_Esin_And2018_Cyb	BOTA12	1	BC29814_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA12	Cytb	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	ACCCCTCCTCATATCCAACCA	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.929	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29814_Mgig_Bot2012_COI	BOTA12	1	BC29814_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	TCTTATTCGTTGGAGACTTTATAACCCT	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29814_Mjap_Gib2022_COI	BOTA12	1	BC29814_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	AATTTAATTGTGACTAGGCATGGTCTG	151.20642
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29815_Cmae_Bot2011_COI	BOTA11	1	BC29815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	ATGAACAGTCTATCCTCCTTTAG	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29815_Hsan_Wil2021_COI	BOTA11	1	BC29815_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	CCTGGGCCGGTATAGTAGGT	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29815_Mros_Gib2022_COI	BOTA11	1	BC29815_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29815_Msal_Bot2012_COI	BOTA11	1	BC29815_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	GYTAGTTCCRATGATGTTAGCTG	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29815_Pvir_Dia2013_COI	BOTA11	1	BC29815_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	CTTAGTGGCATTAATTCGDAATCC	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29815_Rhar_Sim2018_COI	BOTA11	1	BC29815_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	CTGCTGCTATTGCTCATG	151.20777
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29817_Aamu_Bax2006_COI	BOTA9	1	BC29817_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	GCACAACCGGGATCTTTACTTC	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29817_Pcan_Bot2011_IGS	BOTA9	1	BC29817_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA9	IGS	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	CGTAATCCTCAGTACTGGCTA	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29817_Pper_Dia2013_COI	BOTA9	1	BC29817_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	CTTAGTGGCATTAATTCGDAATCC	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29817_Sspa_Oph2007_COI	BOTA9	1	BC29817_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	GAAGGCCTTCCGATGATC	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29817_Upin_Bot2011_COI	BOTA9	1	BC29817_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	CTTTAATTACAGCGTTTTTATTGTTGT	151.21203
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29818_Cjap_Sim2018_COI	BOTA8	1	BC29818_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29818_Esin_And2018_Cyb	BOTA8	1	BC29818_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA8	Cytb	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	ACCCCTCCTCATATCCAACCA	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.004	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29818_Mgig_Bot2012_COI	BOTA8	1	BC29818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	TCTTATTCGTTGGAGACTTTATAACCCT	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29818_Mjap_Gib2022_COI	BOTA8	1	BC29818_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	AATTTAATTGTGACTAGGCATGGTCTG	151.20886
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29819_Cmae_Bot2011_COI	BOTA7	1	BC29819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	ATGAACAGTCTATCCTCCTTTAG	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29819_Hsan_Wil2021_COI	BOTA7	1	BC29819_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	CCTGGGCCGGTATAGTAGGT	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29819_Mros_Gib2022_COI	BOTA7	1	BC29819_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29819_Msal_Bot2012_COI	BOTA7	1	BC29819_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	GYTAGTTCCRATGATGTTAGCTG	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29819_Pvir_Dia2013_COI	BOTA7	1	BC29819_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	CTTAGTGGCATTAATTCGDAATCC	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29819_Rhar_Sim2018_COI	BOTA7	1	BC29819_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	CTGCTGCTATTGCTCATG	151.21359
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29820_Aamu_Bax2006_COI	BOTA6	1	BC29820_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	GCACAACCGGGATCTTTACTTC	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29820_Pcan_Bot2011_IGS	BOTA6	1	BC29820_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA6	IGS	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	CGTAATCCTCAGTACTGGCTA	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29820_Pper_Dia2013_COI	BOTA6	1	BC29820_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	CTTAGTGGCATTAATTCGDAATCC	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29820_Sspa_Oph2007_COI	BOTA6	1	BC29820_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	GAAGGCCTTCCGATGATC	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29820_Upin_Bot2011_COI	BOTA6	1	BC29820_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	CTTTAATTACAGCGTTTTTATTGTTGT	151.20998
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29821_Cjap_Sim2018_COI	BOTA5	1	BC29821_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29821_Esin_And2018_Cyb	BOTA5	1	BC29821_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA5	Cytb	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	ACCCCTCCTCATATCCAACCA	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.077	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29821_Mgig_Bot2012_COI	BOTA5	1	BC29821_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	TCTTATTCGTTGGAGACTTTATAACCCT	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29821_Mjap_Gib2022_COI	BOTA5	1	BC29821_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	AATTTAATTGTGACTAGGCATGGTCTG	151.20803
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29822_Cmae_Bot2011_COI	BOTA4	1	BC29822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	ATGAACAGTCTATCCTCCTTTAG	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29822_Hsan_Wil2021_COI	BOTA4	1	BC29822_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	CCTGGGCCGGTATAGTAGGT	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29822_Mros_Gib2022_COI	BOTA4	1	BC29822_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29822_Msal_Bot2012_COI	BOTA4	1	BC29822_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	GYTAGTTCCRATGATGTTAGCTG	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29822_Pvir_Dia2013_COI	BOTA4	1	BC29822_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	CTTAGTGGCATTAATTCGDAATCC	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29822_Rhar_Sim2018_COI	BOTA4	1	BC29822_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	CTGCTGCTATTGCTCATG	151.21232
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29824_Aamu_Bax2006_COI	BOTA1	1	BC29824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	GCACAACCGGGATCTTTACTTC	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29824_Pcan_Bot2011_IGS	BOTA1	1	BC29824_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA1	IGS	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	CGTAATCCTCAGTACTGGCTA	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29824_Pper_Dia2013_COI	BOTA1	1	BC29824_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	CTTAGTGGCATTAATTCGDAATCC	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29824_Sspa_Oph2007_COI	BOTA1	1	BC29824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	GAAGGCCTTCCGATGATC	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29824_Upin_Bot2011_COI	BOTA1	1	BC29824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	CTTTAATTACAGCGTTTTTATTGTTGT	151.20991
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29825_Cjap_Sim2018_COI	BOTA2	1	BC29825_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29825_Esin_And2018_Cyb	BOTA2	1	BC29825_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA2	Cytb	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	ACCCCTCCTCATATCCAACCA	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.824	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29825_Mgig_Bot2012_COI	BOTA2	1	BC29825_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	TCTTATTCGTTGGAGACTTTATAACCCT	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29825_Mjap_Gib2022_COI	BOTA2	1	BC29825_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	AATTTAATTGTGACTAGGCATGGTCTG	151.21153
BC29826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66552	WGS84	BC29826	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29826_Cmae_Bot2011_COI	WEIA5	1	BC29826_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29826	Sample	BC29826	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA5	COI	45	BC29826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA5	ATGAACAGTCTATCCTCCTTTAG	141.85005
BC29826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66552	WGS84	BC29826	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29826_Msal_Bot2012_COI	WEIA5	1	BC29826_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29826	Sample	BC29826	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA5	COI	45	BC29826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA5	GYTAGTTCCRATGATGTTAGCTG	141.85005
BC29828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67224	WGS84	BC29828	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29828_Aamu_Bax2006_COI	WEIA18	1	BC29828_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29828	Sample	BC29828	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA18	COI	45	BC29828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA18	GCACAACCGGGATCTTTACTTC	141.86659
BC29828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67224	WGS84	BC29828	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29828_Pcan_Bot2011_IGS	WEIA18	1	BC29828_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29828	Sample	BC29828	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA18	IGS	45	BC29828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA18	CGTAATCCTCAGTACTGGCTA	141.86659
BC29828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67224	WGS84	BC29828	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29828_Sspa_Oph2007_COI	WEIA18	1	BC29828_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29828	Sample	BC29828	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA18	COI	45	BC29828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA18	GAAGGCCTTCCGATGATC	141.86659
BC29828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67224	WGS84	BC29828	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29828_Upin_Bot2011_COI	WEIA18	1	BC29828_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29828	Sample	BC29828	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA18	COI	45	BC29828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA18	CTTTAATTACAGCGTTTTTATTGTTGT	141.86659
BC29829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67328	WGS84	BC29829	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29829_Mgig_Bot2012_COI	WEIA17	1	BC29829_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29829	Sample	BC29829	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA17	COI	45	BC29829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA17	TCTTATTCGTTGGAGACTTTATAACCCT	141.87218
BC29831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67395	WGS84	BC29831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29831_Aamu_Bax2006_COI	WEIA14	1	BC29831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29831	Sample	BC29831	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA14	COI	45	BC29831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA14	GCACAACCGGGATCTTTACTTC	141.8665
BC29831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67395	WGS84	BC29831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29831_Pcan_Bot2011_IGS	WEIA14	1	BC29831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29831	Sample	BC29831	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA14	IGS	45	BC29831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA14	CGTAATCCTCAGTACTGGCTA	141.8665
BC29831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67395	WGS84	BC29831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29831_Sspa_Oph2007_COI	WEIA14	1	BC29831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29831	Sample	BC29831	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA14	COI	45	BC29831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA14	GAAGGCCTTCCGATGATC	141.8665
BC29831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67395	WGS84	BC29831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29831_Upin_Bot2011_COI	WEIA14	1	BC29831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29831	Sample	BC29831	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA14	COI	45	BC29831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA14	CTTTAATTACAGCGTTTTTATTGTTGT	141.8665
BC29832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67448	WGS84	BC29832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29832_Mgig_Bot2012_COI	WEIA13	1	BC29832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29832	Sample	BC29832	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA13	COI	45	BC29832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA13	TCTTATTCGTTGGAGACTTTATAACCCT	141.87165
BC29833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67486	WGS84	BC29833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29833_Cmae_Bot2011_COI	WEIA12	1	BC29833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29833	Sample	BC29833	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA12	COI	45	BC29833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA12	ATGAACAGTCTATCCTCCTTTAG	141.87001
BC29833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67486	WGS84	BC29833	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29833_Msal_Bot2012_COI	WEIA12	1	BC29833_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29833	Sample	BC29833	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA12	COI	45	BC29833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA12	GYTAGTTCCRATGATGTTAGCTG	141.87001
BC29835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66819	WGS84	BC29835	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29835_Aamu_Bax2006_COI	WEIA9	1	BC29835_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29835	Sample	BC29835	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA9	COI	45	BC29835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA9	GCACAACCGGGATCTTTACTTC	141.86086
BC29835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66819	WGS84	BC29835	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29835_Pcan_Bot2011_IGS	WEIA9	1	BC29835_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29835	Sample	BC29835	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA9	IGS	45	BC29835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA9	CGTAATCCTCAGTACTGGCTA	141.86086
BC29835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66819	WGS84	BC29835	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29835_Sspa_Oph2007_COI	WEIA9	1	BC29835_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29835	Sample	BC29835	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA9	COI	45	BC29835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA9	GAAGGCCTTCCGATGATC	141.86086
BC29835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66819	WGS84	BC29835	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29835_Upin_Bot2011_COI	WEIA9	1	BC29835_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29835	Sample	BC29835	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA9	COI	45	BC29835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA9	CTTTAATTACAGCGTTTTTATTGTTGT	141.86086
BC29836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66946	WGS84	BC29836	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29836_Mgig_Bot2012_COI	WEIA8	1	BC29836_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29836	Sample	BC29836	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA8	COI	45	BC29836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA8	TCTTATTCGTTGGAGACTTTATAACCCT	141.86323
BC29837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67017	WGS84	BC29837	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29837_Cmae_Bot2011_COI	WEIA7	1	BC29837_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29837	Sample	BC29837	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA7	COI	45	BC29837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA7	ATGAACAGTCTATCCTCCTTTAG	141.86209
BC29837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67017	WGS84	BC29837	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29837_Msal_Bot2012_COI	WEIA7	1	BC29837_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29837	Sample	BC29837	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA7	COI	45	BC29837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA7	GYTAGTTCCRATGATGTTAGCTG	141.86209
BC29839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66715	WGS84	BC29839	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29839_Aamu_Bax2006_COI	WEIA3	1	BC29839_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29839	Sample	BC29839	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA3	COI	45	BC29839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA3	GCACAACCGGGATCTTTACTTC	141.84948
BC29839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66715	WGS84	BC29839	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29839_Pcan_Bot2011_IGS	WEIA3	1	BC29839_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29839	Sample	BC29839	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA3	IGS	45	BC29839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA3	CGTAATCCTCAGTACTGGCTA	141.84948
BC29839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66715	WGS84	BC29839	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29839_Sspa_Oph2007_COI	WEIA3	1	BC29839_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29839	Sample	BC29839	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA3	COI	45	BC29839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA3	GAAGGCCTTCCGATGATC	141.84948
BC29839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66715	WGS84	BC29839	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29839_Upin_Bot2011_COI	WEIA3	1	BC29839_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29839	Sample	BC29839	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA3	COI	45	BC29839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA3	CTTTAATTACAGCGTTTTTATTGTTGT	141.84948
BC29840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.6676	WGS84	BC29840	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29840_Cmae_Bot2011_COI	WEIA1	1	BC29840_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29840	Sample	BC29840	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA1	COI	45	BC29840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA1	ATGAACAGTCTATCCTCCTTTAG	141.846
BC29840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.6676	WGS84	BC29840	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29840_Msal_Bot2012_COI	WEIA1	1	BC29840_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29840	Sample	BC29840	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA1	COI	45	BC29840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA1	GYTAGTTCCRATGATGTTAGCTG	141.846
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20676	WGS84	BC29846	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29846_Aamu_Bax2006_COI	GOVA15	1	BC29846_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	GCACAACCGGGATCTTTACTTC	136.66753
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20676	WGS84	BC29846	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29846_Pcan_Bot2011_IGS	GOVA15	1	BC29846_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA15	IGS	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	CGTAATCCTCAGTACTGGCTA	136.66753
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20676	WGS84	BC29846	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29846_Sspa_Oph2007_COI	GOVA15	1	BC29846_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	GAAGGCCTTCCGATGATC	136.66753
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20676	WGS84	BC29846	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29846_Upin_Bot2011_COI	GOVA15	1	BC29846_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	CTTTAATTACAGCGTTTTTATTGTTGT	136.66753
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-12.20676	WGS84	BC29846	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29846_Vgib_Wil2025_COI	GOVA15	1	BC29846_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Vgib_Wil2025_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	CCCCTCTTTACAAGTTCTTTAGGGT	136.66753
BC29847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20909	WGS84	BC29847	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29847_Mgig_Bot2012_COI	GOVA10	1	BC29847_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29847	Sample	BC29847	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA10	COI	45	BC29847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA10	TCTTATTCGTTGGAGACTTTATAACCCT	136.66891
BC29848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20461	WGS84	BC29848	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29848_Cmae_Bot2011_COI	GOVA5	1	BC29848_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29848	Sample	BC29848	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA5	COI	45	BC29848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA5	ATGAACAGTCTATCCTCCTTTAG	136.68266
BC29848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20461	WGS84	BC29848	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29848_Msal_Bot2012_COI	GOVA5	1	BC29848_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29848	Sample	BC29848	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA5	COI	45	BC29848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA5	GYTAGTTCCRATGATGTTAGCTG	136.68266
BC29850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20397	WGS84	BC29850	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29850_Mgig_Bot2012_COI	GOVA21	1	BC29850_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29850	Sample	BC29850	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA21	COI	45	BC29850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA21	TCTTATTCGTTGGAGACTTTATAACCCT	136.67036
BC29851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20509	WGS84	BC29851	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29851_Cmae_Bot2011_COI	GOVA19	1	BC29851_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29851	Sample	BC29851	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA19	COI	45	BC29851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA19	ATGAACAGTCTATCCTCCTTTAG	136.66844
BC29851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20509	WGS84	BC29851	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29851_Msal_Bot2012_COI	GOVA19	1	BC29851_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29851	Sample	BC29851	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA19	COI	45	BC29851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA19	GYTAGTTCCRATGATGTTAGCTG	136.66844
BC29853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20777	WGS84	BC29853	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29853_Aamu_Bax2006_COI	GOVA13	1	BC29853_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29853	Sample	BC29853	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA13	COI	45	BC29853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA13	GCACAACCGGGATCTTTACTTC	136.66944
BC29853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20777	WGS84	BC29853	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29853_Pcan_Bot2011_IGS	GOVA13	1	BC29853_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29853	Sample	BC29853	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA13	IGS	45	BC29853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA13	CGTAATCCTCAGTACTGGCTA	136.66944
BC29853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20777	WGS84	BC29853	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29853_Sspa_Oph2007_COI	GOVA13	1	BC29853_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29853	Sample	BC29853	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA13	COI	45	BC29853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA13	GAAGGCCTTCCGATGATC	136.66944
BC29853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20777	WGS84	BC29853	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29853_Upin_Bot2011_COI	GOVA13	1	BC29853_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29853	Sample	BC29853	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA13	COI	45	BC29853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA13	CTTTAATTACAGCGTTTTTATTGTTGT	136.66944
BC29854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20891	WGS84	BC29854	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29854_Mgig_Bot2012_COI	GOVA11	1	BC29854_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29854	Sample	BC29854	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA11	COI	45	BC29854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA11	TCTTATTCGTTGGAGACTTTATAACCCT	136.67007
BC29855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.21015	WGS84	BC29855	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29855_Cmae_Bot2011_COI	GOVA9	1	BC29855_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29855	Sample	BC29855	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA9	COI	45	BC29855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA9	ATGAACAGTCTATCCTCCTTTAG	136.67167
BC29855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.21015	WGS84	BC29855	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29855_Msal_Bot2012_COI	GOVA9	1	BC29855_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29855	Sample	BC29855	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA9	COI	45	BC29855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA9	GYTAGTTCCRATGATGTTAGCTG	136.67167
BC29857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.2028	WGS84	BC29857	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29857_Aamu_Bax2006_COI	GOVA6	1	BC29857_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29857	Sample	BC29857	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA6	COI	45	BC29857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA6	GCACAACCGGGATCTTTACTTC	136.68155
BC29857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.2028	WGS84	BC29857	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29857_Pcan_Bot2011_IGS	GOVA6	1	BC29857_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29857	Sample	BC29857	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA6	IGS	45	BC29857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA6	CGTAATCCTCAGTACTGGCTA	136.68155
BC29857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.2028	WGS84	BC29857	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29857_Sspa_Oph2007_COI	GOVA6	1	BC29857_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29857	Sample	BC29857	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA6	COI	45	BC29857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA6	GAAGGCCTTCCGATGATC	136.68155
BC29857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.2028	WGS84	BC29857	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29857_Upin_Bot2011_COI	GOVA6	1	BC29857_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29857	Sample	BC29857	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA6	COI	45	BC29857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA6	CTTTAATTACAGCGTTTTTATTGTTGT	136.68155
BC29858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20648	WGS84	BC29858	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29858_Mgig_Bot2012_COI	GOVA3	1	BC29858_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29858	Sample	BC29858	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA3	COI	45	BC29858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA3	TCTTATTCGTTGGAGACTTTATAACCCT	136.68
BC29859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19561	WGS84	BC29859	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29859_Cmae_Bot2011_COI	GOVA2	1	BC29859_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29859	Sample	BC29859	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA2	COI	45	BC29859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA2	ATGAACAGTCTATCCTCCTTTAG	136.68673
BC29859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19561	WGS84	BC29859	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29859_Msal_Bot2012_COI	GOVA2	1	BC29859_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29859	Sample	BC29859	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA2	COI	45	BC29859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA2	GYTAGTTCCRATGATGTTAGCTG	136.68673
BC29860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19556	WGS84	BC29860	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29860_Aamu_Bax2006_COI	GOVA1	1	BC29860_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29860	Sample	BC29860	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA1	COI	45	BC29860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA1	GCACAACCGGGATCTTTACTTC	136.68512
BC29860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19556	WGS84	BC29860	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29860_Pcan_Bot2011_IGS	GOVA1	1	BC29860_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29860	Sample	BC29860	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA1	IGS	45	BC29860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA1	CGTAATCCTCAGTACTGGCTA	136.68512
BC29860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19556	WGS84	BC29860	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29860_Sspa_Oph2007_COI	GOVA1	1	BC29860_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29860	Sample	BC29860	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA1	COI	45	BC29860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA1	GAAGGCCTTCCGATGATC	136.68512
BC29860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19556	WGS84	BC29860	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29860_Upin_Bot2011_COI	GOVA1	1	BC29860_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29860	Sample	BC29860	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA1	COI	45	BC29860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA1	CTTTAATTACAGCGTTTTTATTGTTGT	136.68512
BC37388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99816	WGS84	BC37388	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37388_Aamu_Bax2006_COI	BOTB7	1	BC37388_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37388	Sample	BC37388	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB7	COI	45	BC37388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB7	GCACAACCGGGATCTTTACTTC	151.21204
BC37388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99816	WGS84	BC37388	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37388_Pcan_Bot2011_IGS	BOTB7	1	BC37388_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37388	Sample	BC37388	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB7	IGS	45	BC37388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB7	CGTAATCCTCAGTACTGGCTA	151.21204
BC37388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99816	WGS84	BC37388	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37388_Sspa_Oph2007_COI	BOTB7	1	BC37388_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37388	Sample	BC37388	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB7	COI	45	BC37388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB7	GAAGGCCTTCCGATGATC	151.21204
BC37388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99816	WGS84	BC37388	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37388_Upin_Bot2011_COI	BOTB7	1	BC37388_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37388	Sample	BC37388	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB7	COI	45	BC37388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB7	CTTTAATTACAGCGTTTTTATTGTTGT	151.21204
BC37389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99727	WGS84	BC37389	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37389_Mgig_Bot2012_COI	BOTB8	1	BC37389_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37389	Sample	BC37389	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB8	COI	45	BC37389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB8	TCTTATTCGTTGGAGACTTTATAACCCT	151.20967
BC37391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98273	WGS84	BC37391	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37391_Aamu_Bax2006_COI	BOTB10	1	BC37391_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37391	Sample	BC37391	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB10	COI	45	BC37391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB10	GCACAACCGGGATCTTTACTTC	151.20824
BC37391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98273	WGS84	BC37391	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37391_Pcan_Bot2011_IGS	BOTB10	1	BC37391_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37391	Sample	BC37391	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB10	IGS	45	BC37391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB10	CGTAATCCTCAGTACTGGCTA	151.20824
BC37391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98273	WGS84	BC37391	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.287	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37391_Sspa_Oph2007_COI	BOTB10	1	BC37391_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37391	Sample	BC37391	targeted	Present	Sample	Sspa_Oph2007_COI	BOTB10	COI	45	BC37391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB10	GAAGGCCTTCCGATGATC	151.20824
BC37391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98273	WGS84	BC37391	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37391_Upin_Bot2011_COI	BOTB10	1	BC37391_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37391	Sample	BC37391	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB10	COI	45	BC37391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB10	CTTTAATTACAGCGTTTTTATTGTTGT	151.20824
BC37392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98116	WGS84	BC37392	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37392_Mgig_Bot2012_COI	BOTB11	1	BC37392_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37392	Sample	BC37392	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB11	COI	45	BC37392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB11	TCTTATTCGTTGGAGACTTTATAACCCT	151.20848
BC37393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97923	WGS84	BC37393	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37393_Cmae_Bot2011_COI	BOTB12	1	BC37393_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37393	Sample	BC37393	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB12	COI	45	BC37393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB12	ATGAACAGTCTATCCTCCTTTAG	151.20787
BC37393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97923	WGS84	BC37393	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37393_Msal_Bot2012_COI	BOTB12	1	BC37393_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37393	Sample	BC37393	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB12	COI	45	BC37393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB12	GYTAGTTCCRATGATGTTAGCTG	151.20787
BC37395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9773	WGS84	BC37395	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37395_Aamu_Bax2006_COI	BOTB14	1	BC37395_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37395	Sample	BC37395	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB14	COI	45	BC37395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB14	GCACAACCGGGATCTTTACTTC	151.20861
BC37395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9773	WGS84	BC37395	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37395_Pcan_Bot2011_IGS	BOTB14	1	BC37395_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37395	Sample	BC37395	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB14	IGS	45	BC37395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB14	CGTAATCCTCAGTACTGGCTA	151.20861
BC37395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9773	WGS84	BC37395	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37395_Sspa_Oph2007_COI	BOTB14	1	BC37395_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37395	Sample	BC37395	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB14	COI	45	BC37395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB14	GAAGGCCTTCCGATGATC	151.20861
BC37395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9773	WGS84	BC37395	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37395_Upin_Bot2011_COI	BOTB14	1	BC37395_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37395	Sample	BC37395	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB14	COI	45	BC37395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB14	CTTTAATTACAGCGTTTTTATTGTTGT	151.20861
BC37396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97617	WGS84	BC37396	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37396_Mgig_Bot2012_COI	BOTB15	1	BC37396_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37396	Sample	BC37396	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB15	COI	45	BC37396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB15	TCTTATTCGTTGGAGACTTTATAACCCT	151.20929
BC37397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97578	WGS84	BC37397	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37397_Cmae_Bot2011_COI	BOTB16	1	BC37397_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37397	Sample	BC37397	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB16	COI	45	BC37397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB16	ATGAACAGTCTATCCTCCTTTAG	151.2104
BC37397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97578	WGS84	BC37397	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37397_Msal_Bot2012_COI	BOTB16	1	BC37397_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37397	Sample	BC37397	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB16	COI	45	BC37397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB16	GYTAGTTCCRATGATGTTAGCTG	151.2104
BC37399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97298	WGS84	BC37399	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37399_Aamu_Bax2006_COI	BOTB18	1	BC37399_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37399	Sample	BC37399	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB18	COI	45	BC37399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB18	GCACAACCGGGATCTTTACTTC	151.20234
BC37399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97298	WGS84	BC37399	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37399_Pcan_Bot2011_IGS	BOTB18	1	BC37399_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37399	Sample	BC37399	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB18	IGS	45	BC37399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB18	CGTAATCCTCAGTACTGGCTA	151.20234
BC37399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97298	WGS84	BC37399	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37399_Sspa_Oph2007_COI	BOTB18	1	BC37399_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37399	Sample	BC37399	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB18	COI	45	BC37399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB18	GAAGGCCTTCCGATGATC	151.20234
BC37399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97298	WGS84	BC37399	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37399_Upin_Bot2011_COI	BOTB18	1	BC37399_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37399	Sample	BC37399	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB18	COI	45	BC37399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB18	CTTTAATTACAGCGTTTTTATTGTTGT	151.20234
BC37400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97257	WGS84	BC37400	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37400_Mgig_Bot2012_COI	BOTB19	1	BC37400_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37400	Sample	BC37400	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB19	COI	45	BC37400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB19	TCTTATTCGTTGGAGACTTTATAACCCT	151.20432
BC37401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97244	WGS84	BC37401	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37401_Cmae_Bot2011_COI	BOTB20	1	BC37401_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37401	Sample	BC37401	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB20	COI	45	BC37401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB20	ATGAACAGTCTATCCTCCTTTAG	151.20641
BC37401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97244	WGS84	BC37401	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37401_Msal_Bot2012_COI	BOTB20	1	BC37401_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37401	Sample	BC37401	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB20	COI	45	BC37401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB20	GYTAGTTCCRATGATGTTAGCTG	151.20641
BC37403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97278	WGS84	BC37403	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37403_Aamu_Bax2006_COI	BOTB22	1	BC37403_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37403	Sample	BC37403	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB22	COI	45	BC37403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB22	GCACAACCGGGATCTTTACTTC	151.21048
BC37403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97278	WGS84	BC37403	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37403_Pcan_Bot2011_IGS	BOTB22	1	BC37403_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37403	Sample	BC37403	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB22	IGS	45	BC37403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB22	CGTAATCCTCAGTACTGGCTA	151.21048
BC37403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97278	WGS84	BC37403	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37403_Sspa_Oph2007_COI	BOTB22	1	BC37403_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37403	Sample	BC37403	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB22	COI	45	BC37403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB22	GAAGGCCTTCCGATGATC	151.21048
BC37403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97278	WGS84	BC37403	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37403_Upin_Bot2011_COI	BOTB22	1	BC37403_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37403	Sample	BC37403	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB22	COI	45	BC37403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB22	CTTTAATTACAGCGTTTTTATTGTTGT	151.21048
BC37404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97176	WGS84	BC37404	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37404_Mgig_Bot2012_COI	BOTB23	1	BC37404_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37404	Sample	BC37404	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB23	COI	45	BC37404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB23	TCTTATTCGTTGGAGACTTTATAACCCT	151.19977
BC37405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97152	WGS84	BC37405	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37405_Cmae_Bot2011_COI	BOTB24	1	BC37405_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37405	Sample	BC37405	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB24	COI	45	BC37405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB24	ATGAACAGTCTATCCTCCTTTAG	151.20333
BC37405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97152	WGS84	BC37405	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37405_Msal_Bot2012_COI	BOTB24	1	BC37405_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37405	Sample	BC37405	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB24	COI	45	BC37405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB24	GYTAGTTCCRATGATGTTAGCTG	151.20333
BC37407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97093	WGS84	BC37407	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37407_Aamu_Bax2006_COI	BOTB26	1	BC37407_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37407	Sample	BC37407	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB26	COI	45	BC37407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB26	GCACAACCGGGATCTTTACTTC	151.20754
BC37407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97093	WGS84	BC37407	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37407_Pcan_Bot2011_IGS	BOTB26	1	BC37407_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37407	Sample	BC37407	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB26	IGS	45	BC37407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB26	CGTAATCCTCAGTACTGGCTA	151.20754
BC37407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97093	WGS84	BC37407	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37407_Sspa_Oph2007_COI	BOTB26	1	BC37407_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37407	Sample	BC37407	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB26	COI	45	BC37407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB26	GAAGGCCTTCCGATGATC	151.20754
BC37407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97093	WGS84	BC37407	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37407_Upin_Bot2011_COI	BOTB26	1	BC37407_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37407	Sample	BC37407	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB26	COI	45	BC37407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB26	CTTTAATTACAGCGTTTTTATTGTTGT	151.20754
BC37408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97067	WGS84	BC37408	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37408_Mgig_Bot2012_COI	BOTB27	1	BC37408_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37408	Sample	BC37408	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB27	COI	45	BC37408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB27	TCTTATTCGTTGGAGACTTTATAACCCT	151.20974
BC37409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97049	WGS84	BC37409	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37409_Cmae_Bot2011_COI	BOTB28	1	BC37409_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37409	Sample	BC37409	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB28	COI	45	BC37409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB28	ATGAACAGTCTATCCTCCTTTAG	151.21199
BC37409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97049	WGS84	BC37409	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37409_Msal_Bot2012_COI	BOTB28	1	BC37409_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37409	Sample	BC37409	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB28	COI	45	BC37409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB28	GYTAGTTCCRATGATGTTAGCTG	151.21199
BC37410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97018	WGS84	BC37410	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37410_Aamu_Bax2006_COI	BOTB29	1	BC37410_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37410	Sample	BC37410	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB29	COI	45	BC37410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB29	GCACAACCGGGATCTTTACTTC	151.21416
BC37410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97018	WGS84	BC37410	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37410_Pcan_Bot2011_IGS	BOTB29	1	BC37410_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37410	Sample	BC37410	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB29	IGS	45	BC37410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB29	CGTAATCCTCAGTACTGGCTA	151.21416
BC37410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97018	WGS84	BC37410	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37410_Sspa_Oph2007_COI	BOTB29	1	BC37410_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37410	Sample	BC37410	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB29	COI	45	BC37410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB29	GAAGGCCTTCCGATGATC	151.21416
BC37410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97018	WGS84	BC37410	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37410_Upin_Bot2011_COI	BOTB29	1	BC37410_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37410	Sample	BC37410	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB29	COI	45	BC37410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB29	CTTTAATTACAGCGTTTTTATTGTTGT	151.21416
BC37411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96986	WGS84	BC37411	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37411_Mgig_Bot2012_COI	BOTB30	1	BC37411_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37411	Sample	BC37411	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB30	COI	45	BC37411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB30	TCTTATTCGTTGGAGACTTTATAACCCT	151.21632
BC37412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97019	WGS84	BC37412	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37412_Cmae_Bot2011_COI	BOTB31	1	BC37412_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37412	Sample	BC37412	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB31	COI	45	BC37412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB31	ATGAACAGTCTATCCTCCTTTAG	151.19852
BC37412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97019	WGS84	BC37412	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37412_Msal_Bot2012_COI	BOTB31	1	BC37412_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37412	Sample	BC37412	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB31	COI	45	BC37412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB31	GYTAGTTCCRATGATGTTAGCTG	151.19852
BC37414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9686	WGS84	BC37414	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37414_Aamu_Bax2006_COI	BOTB33	1	BC37414_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37414	Sample	BC37414	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB33	COI	45	BC37414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB33	GCACAACCGGGATCTTTACTTC	151.21507
BC37414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9686	WGS84	BC37414	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37414_Pcan_Bot2011_IGS	BOTB33	1	BC37414_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37414	Sample	BC37414	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB33	IGS	45	BC37414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB33	CGTAATCCTCAGTACTGGCTA	151.21507
BC37414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9686	WGS84	BC37414	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37414_Sspa_Oph2007_COI	BOTB33	1	BC37414_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37414	Sample	BC37414	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB33	COI	45	BC37414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB33	GAAGGCCTTCCGATGATC	151.21507
BC37414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9686	WGS84	BC37414	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37414_Upin_Bot2011_COI	BOTB33	1	BC37414_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37414	Sample	BC37414	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB33	COI	45	BC37414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB33	CTTTAATTACAGCGTTTTTATTGTTGT	151.21507
BC37415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9682	WGS84	BC37415	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37415_Mgig_Bot2012_COI	BOTB34	1	BC37415_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37415	Sample	BC37415	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB34	COI	45	BC37415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB34	TCTTATTCGTTGGAGACTTTATAACCCT	151.21721
BC37416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96834	WGS84	BC37416	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37416_Cmae_Bot2011_COI	BOTB35	1	BC37416_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37416	Sample	BC37416	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB35	COI	45	BC37416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB35	ATGAACAGTCTATCCTCCTTTAG	151.21836
BC37416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96834	WGS84	BC37416	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37416_Msal_Bot2012_COI	BOTB35	1	BC37416_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37416	Sample	BC37416	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB35	COI	45	BC37416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB35	GYTAGTTCCRATGATGTTAGCTG	151.21836
BC37418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96688	WGS84	BC37418	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37418_Aamu_Bax2006_COI	BOTB37	1	BC37418_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37418	Sample	BC37418	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB37	COI	45	BC37418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB37	GCACAACCGGGATCTTTACTTC	151.19813
BC37418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96688	WGS84	BC37418	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37418_Pcan_Bot2011_IGS	BOTB37	1	BC37418_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37418	Sample	BC37418	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB37	IGS	45	BC37418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB37	CGTAATCCTCAGTACTGGCTA	151.19813
BC37418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96688	WGS84	BC37418	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37418_Sspa_Oph2007_COI	BOTB37	1	BC37418_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37418	Sample	BC37418	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB37	COI	45	BC37418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB37	GAAGGCCTTCCGATGATC	151.19813
BC37418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96688	WGS84	BC37418	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37418_Upin_Bot2011_COI	BOTB37	1	BC37418_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37418	Sample	BC37418	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB37	COI	45	BC37418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB37	CTTTAATTACAGCGTTTTTATTGTTGT	151.19813
BC37419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96582	WGS84	BC37419	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37419_Mgig_Bot2012_COI	BOTB38	1	BC37419_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37419	Sample	BC37419	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB38	COI	45	BC37419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB38	TCTTATTCGTTGGAGACTTTATAACCCT	151.19983
BC37421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.95928	WGS84	BC37421	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37421_Aamu_Bax2006_COI	BOTB42	1	BC37421_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37421	Sample	BC37421	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB42	COI	45	BC37421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB42	GCACAACCGGGATCTTTACTTC	151.19883
BC37421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.95928	WGS84	BC37421	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37421_Pcan_Bot2011_IGS	BOTB42	1	BC37421_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37421	Sample	BC37421	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB42	IGS	45	BC37421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB42	CGTAATCCTCAGTACTGGCTA	151.19883
BC37421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.95928	WGS84	BC37421	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37421_Sspa_Oph2007_COI	BOTB42	1	BC37421_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37421	Sample	BC37421	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB42	COI	45	BC37421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB42	GAAGGCCTTCCGATGATC	151.19883
BC37421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.95928	WGS84	BC37421	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37421_Upin_Bot2011_COI	BOTB42	1	BC37421_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37421	Sample	BC37421	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB42	COI	45	BC37421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB42	CTTTAATTACAGCGTTTTTATTGTTGT	151.19883
BC37422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96071	WGS84	BC37422	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.817	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37422_Mgig_Bot2012_COI	BOTB41	1	BC37422_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37422	Sample	BC37422	targeted	Present	Sample	Mgig_Bot2012_COI	BOTB41	COI	45	BC37422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB41	TCTTATTCGTTGGAGACTTTATAACCCT	151.19803
BC37426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8814	WGS84	BC37426	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.926	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37426_Mgig_Bot2012_COI	NEWB46	1	BC37426_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37426	Sample	BC37426	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB46	COI	45	BC37426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB46	TCTTATTCGTTGGAGACTTTATAACCCT	151.75162
BC37427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88148	WGS84	BC37427	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37427_Cmae_Bot2011_COI	NEWB45	1	BC37427_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37427	Sample	BC37427	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB45	COI	45	BC37427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB45	ATGAACAGTCTATCCTCCTTTAG	151.74943
BC37427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88148	WGS84	BC37427	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37427_Msal_Bot2012_COI	NEWB45	1	BC37427_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37427	Sample	BC37427	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB45	COI	45	BC37427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB45	GYTAGTTCCRATGATGTTAGCTG	151.74943
BC37429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88427	WGS84	BC37429	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37429_Aamu_Bax2006_COI	NEWB43	1	BC37429_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37429	Sample	BC37429	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB43	COI	45	BC37429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB43	GCACAACCGGGATCTTTACTTC	151.75678
BC37429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88427	WGS84	BC37429	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37429_Pcan_Bot2011_IGS	NEWB43	1	BC37429_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37429	Sample	BC37429	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB43	IGS	45	BC37429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB43	CGTAATCCTCAGTACTGGCTA	151.75678
BC37429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88427	WGS84	BC37429	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37429_Sspa_Oph2007_COI	NEWB43	1	BC37429_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37429	Sample	BC37429	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB43	COI	45	BC37429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB43	GAAGGCCTTCCGATGATC	151.75678
BC37429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88427	WGS84	BC37429	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37429_Upin_Bot2011_COI	NEWB43	1	BC37429_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37429	Sample	BC37429	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB43	COI	45	BC37429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB43	CTTTAATTACAGCGTTTTTATTGTTGT	151.75678
BC37430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88439	WGS84	BC37430	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37430_Cmae_Bot2011_COI	NEWB42	1	BC37430_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37430	Sample	BC37430	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB42	COI	45	BC37430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB42	ATGAACAGTCTATCCTCCTTTAG	151.75453
BC37430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88439	WGS84	BC37430	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37430_Msal_Bot2012_COI	NEWB42	1	BC37430_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37430	Sample	BC37430	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB42	COI	45	BC37430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB42	GYTAGTTCCRATGATGTTAGCTG	151.75453
BC37432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88519	WGS84	BC37432	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37432_Aamu_Bax2006_COI	NEWB40	1	BC37432_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37432	Sample	BC37432	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB40	COI	45	BC37432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB40	GCACAACCGGGATCTTTACTTC	151.75593
BC37432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88519	WGS84	BC37432	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37432_Pcan_Bot2011_IGS	NEWB40	1	BC37432_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37432	Sample	BC37432	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB40	IGS	45	BC37432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB40	CGTAATCCTCAGTACTGGCTA	151.75593
BC37432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88519	WGS84	BC37432	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37432_Sspa_Oph2007_COI	NEWB40	1	BC37432_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37432	Sample	BC37432	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB40	COI	45	BC37432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB40	GAAGGCCTTCCGATGATC	151.75593
BC37432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88519	WGS84	BC37432	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37432_Upin_Bot2011_COI	NEWB40	1	BC37432_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37432	Sample	BC37432	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB40	COI	45	BC37432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB40	CTTTAATTACAGCGTTTTTATTGTTGT	151.75593
BC37433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88695	WGS84	BC37433	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.675	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37433_Mgig_Bot2012_COI	NEWB39	1	BC37433_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37433	Sample	BC37433	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB39	COI	45	BC37433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB39	TCTTATTCGTTGGAGACTTTATAACCCT	151.76199
BC37434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8867	WGS84	BC37434	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37434_Cmae_Bot2011_COI	NEWB38	1	BC37434_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37434	Sample	BC37434	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB38	COI	45	BC37434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB38	ATGAACAGTCTATCCTCCTTTAG	151.75959
BC37434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8867	WGS84	BC37434	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37434_Msal_Bot2012_COI	NEWB38	1	BC37434_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37434	Sample	BC37434	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB38	COI	45	BC37434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB38	GYTAGTTCCRATGATGTTAGCTG	151.75959
BC37436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88799	WGS84	BC37436	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37436_Aamu_Bax2006_COI	NEWB36	1	BC37436_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37436	Sample	BC37436	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB36	COI	45	BC37436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB36	GCACAACCGGGATCTTTACTTC	151.76335
BC37436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88799	WGS84	BC37436	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37436_Pcan_Bot2011_IGS	NEWB36	1	BC37436_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37436	Sample	BC37436	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB36	IGS	45	BC37436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB36	CGTAATCCTCAGTACTGGCTA	151.76335
BC37436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88799	WGS84	BC37436	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37436_Sspa_Oph2007_COI	NEWB36	1	BC37436_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37436	Sample	BC37436	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB36	COI	45	BC37436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB36	GAAGGCCTTCCGATGATC	151.76335
BC37436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88799	WGS84	BC37436	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37436_Upin_Bot2011_COI	NEWB36	1	BC37436_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37436	Sample	BC37436	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB36	COI	45	BC37436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB36	CTTTAATTACAGCGTTTTTATTGTTGT	151.76335
BC37437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8881	WGS84	BC37437	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.504	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37437_Mgig_Bot2012_COI	NEWB35	1	BC37437_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37437	Sample	BC37437	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB35	COI	45	BC37437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB35	TCTTATTCGTTGGAGACTTTATAACCCT	151.7608
BC37438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88925	WGS84	BC37438	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37438_Cmae_Bot2011_COI	NEWB34	1	BC37438_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37438	Sample	BC37438	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB34	COI	45	BC37438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB34	ATGAACAGTCTATCCTCCTTTAG	151.76953
BC37438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88925	WGS84	BC37438	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37438_Msal_Bot2012_COI	NEWB34	1	BC37438_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37438	Sample	BC37438	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB34	COI	45	BC37438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB34	GYTAGTTCCRATGATGTTAGCTG	151.76953
BC37440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88948	WGS84	BC37440	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.147	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37440_Mgig_Bot2012_COI	NEWB32	1	BC37440_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37440	Sample	BC37440	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB32	COI	45	BC37440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB32	TCTTATTCGTTGGAGACTTTATAACCCT	151.76464
BC37441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89028	WGS84	BC37441	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37441_Cmae_Bot2011_COI	NEWB31	1	BC37441_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37441	Sample	BC37441	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB31	COI	45	BC37441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB31	ATGAACAGTCTATCCTCCTTTAG	151.77213
BC37441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89028	WGS84	BC37441	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37441_Msal_Bot2012_COI	NEWB31	1	BC37441_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37441	Sample	BC37441	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB31	COI	45	BC37441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB31	GYTAGTTCCRATGATGTTAGCTG	151.77213
BC37443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89124	WGS84	BC37443	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37443_Aamu_Bax2006_COI	NEWB29	1	BC37443_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37443	Sample	BC37443	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB29	COI	45	BC37443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB29	GCACAACCGGGATCTTTACTTC	151.76864
BC37443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89124	WGS84	BC37443	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37443_Pcan_Bot2011_IGS	NEWB29	1	BC37443_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37443	Sample	BC37443	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB29	IGS	45	BC37443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB29	CGTAATCCTCAGTACTGGCTA	151.76864
BC37443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89124	WGS84	BC37443	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37443_Sspa_Oph2007_COI	NEWB29	1	BC37443_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37443	Sample	BC37443	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB29	COI	45	BC37443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB29	GAAGGCCTTCCGATGATC	151.76864
BC37443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89124	WGS84	BC37443	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37443_Upin_Bot2011_COI	NEWB29	1	BC37443_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37443	Sample	BC37443	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB29	COI	45	BC37443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB29	CTTTAATTACAGCGTTTTTATTGTTGT	151.76864
BC37444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89102	WGS84	BC37444	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.178	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37444_Mgig_Bot2012_COI	NEWB28	1	BC37444_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37444	Sample	BC37444	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB28	COI	45	BC37444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB28	TCTTATTCGTTGGAGACTTTATAACCCT	151.76518
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37445_Cmae_Bot2011_COI	NEWB27	1	BC37445_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	ATGAACAGTCTATCCTCCTTTAG	151.77239
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37445_Msal_Bot2012_COI	NEWB27	1	BC37445_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	GYTAGTTCCRATGATGTTAGCTG	151.77239
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37447_Aamu_Bax2006_COI	NEWB25	1	BC37447_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	GCACAACCGGGATCTTTACTTC	151.76678
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37447_Pcan_Bot2011_IGS	NEWB25	1	BC37447_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB25	IGS	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	CGTAATCCTCAGTACTGGCTA	151.76678
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37447_Sspa_Oph2007_COI	NEWB25	1	BC37447_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	GAAGGCCTTCCGATGATC	151.76678
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37447_Upin_Bot2011_COI	NEWB25	1	BC37447_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	CTTTAATTACAGCGTTTTTATTGTTGT	151.76678
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37447_Vgib_Wil2025_COI	NEWB25	1	BC37447_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	CCCCTCTTTACAAGTTCTTTAGGGT	151.76678
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37448_Asen_Wil2025_COI	NEWB24	1	BC37448_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	GTTGTTGTTACAACACATGCTCTTTTG	151.77132
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37448_Mgig_Bot2012_COI	NEWB24	1	BC37448_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	TCTTATTCGTTGGAGACTTTATAACCCT	151.77132
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37449_Cmae_Bot2011_COI	NEWB23	1	BC37449_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	ATGAACAGTCTATCCTCCTTTAG	151.76928
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37449_Msal_Bot2012_COI	NEWB23	1	BC37449_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	GYTAGTTCCRATGATGTTAGCTG	151.76928
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37450_Aamu_Bax2006_COI	NEWB22	1	BC37450_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	GCACAACCGGGATCTTTACTTC	151.77257
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37450_Pcan_Bot2011_IGS	NEWB22	1	BC37450_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB22	IGS	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	CGTAATCCTCAGTACTGGCTA	151.77257
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37450_Sspa_Oph2007_COI	NEWB22	1	BC37450_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	GAAGGCCTTCCGATGATC	151.77257
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37450_Upin_Bot2011_COI	NEWB22	1	BC37450_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	CTTTAATTACAGCGTTTTTATTGTTGT	151.77257
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37450_Vgib_Wil2025_COI	NEWB22	1	BC37450_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	CCCCTCTTTACAAGTTCTTTAGGGT	151.77257
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37451_Asen_Wil2025_COI	NEWB21	1	BC37451_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	GTTGTTGTTACAACACATGCTCTTTTG	151.76998
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.18	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37451_Mgig_Bot2012_COI	NEWB21	1	BC37451_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	TCTTATTCGTTGGAGACTTTATAACCCT	151.76998
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37452_Cmae_Bot2011_COI	NEWB20	1	BC37452_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	ATGAACAGTCTATCCTCCTTTAG	151.77454
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37452_Msal_Bot2012_COI	NEWB20	1	BC37452_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	GYTAGTTCCRATGATGTTAGCTG	151.77454
BC37454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91478	WGS84	BC37454	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37454_Aamu_Bax2006_COI	NEWB18	1	BC37454_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37454	Sample	BC37454	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB18	COI	45	BC37454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB18	GCACAACCGGGATCTTTACTTC	151.77479
BC37454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91478	WGS84	BC37454	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37454_Pcan_Bot2011_IGS	NEWB18	1	BC37454_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37454	Sample	BC37454	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB18	IGS	45	BC37454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB18	CGTAATCCTCAGTACTGGCTA	151.77479
BC37454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91478	WGS84	BC37454	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37454_Sspa_Oph2007_COI	NEWB18	1	BC37454_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37454	Sample	BC37454	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB18	COI	45	BC37454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB18	GAAGGCCTTCCGATGATC	151.77479
BC37454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91478	WGS84	BC37454	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37454_Upin_Bot2011_COI	NEWB18	1	BC37454_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37454	Sample	BC37454	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB18	COI	45	BC37454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB18	CTTTAATTACAGCGTTTTTATTGTTGT	151.77479
BC37455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91641	WGS84	BC37455	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.636	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37455_Mgig_Bot2012_COI	NEWB17	1	BC37455_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37455	Sample	BC37455	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB17	COI	45	BC37455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB17	TCTTATTCGTTGGAGACTTTATAACCCT	151.77645
BC37456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91743	WGS84	BC37456	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37456_Cmae_Bot2011_COI	NEWB16	1	BC37456_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37456	Sample	BC37456	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB16	COI	45	BC37456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB16	ATGAACAGTCTATCCTCCTTTAG	151.77377
BC37456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91743	WGS84	BC37456	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37456_Msal_Bot2012_COI	NEWB16	1	BC37456_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37456	Sample	BC37456	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB16	COI	45	BC37456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB16	GYTAGTTCCRATGATGTTAGCTG	151.77377
BC37458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91994	WGS84	BC37458	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37458_Aamu_Bax2006_COI	NEWB14	1	BC37458_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37458	Sample	BC37458	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB14	COI	45	BC37458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB14	GCACAACCGGGATCTTTACTTC	151.77723
BC37458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91994	WGS84	BC37458	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37458_Pcan_Bot2011_IGS	NEWB14	1	BC37458_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37458	Sample	BC37458	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB14	IGS	45	BC37458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB14	CGTAATCCTCAGTACTGGCTA	151.77723
BC37458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91994	WGS84	BC37458	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37458_Sspa_Oph2007_COI	NEWB14	1	BC37458_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37458	Sample	BC37458	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB14	COI	45	BC37458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB14	GAAGGCCTTCCGATGATC	151.77723
BC37458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91994	WGS84	BC37458	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37458_Upin_Bot2011_COI	NEWB14	1	BC37458_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37458	Sample	BC37458	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB14	COI	45	BC37458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB14	CTTTAATTACAGCGTTTTTATTGTTGT	151.77723
BC37459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89868	WGS84	BC37459	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37459_Mgig_Bot2012_COI	NEWB13	1	BC37459_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37459	Sample	BC37459	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB13	COI	45	BC37459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB13	TCTTATTCGTTGGAGACTTTATAACCCT	151.77045
BC37461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90277	WGS84	BC37461	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37461_Aamu_Bax2006_COI	NEWB11	1	BC37461_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37461	Sample	BC37461	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB11	COI	45	BC37461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB11	GCACAACCGGGATCTTTACTTC	151.77329
BC37461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90277	WGS84	BC37461	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37461_Pcan_Bot2011_IGS	NEWB11	1	BC37461_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37461	Sample	BC37461	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB11	IGS	45	BC37461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB11	CGTAATCCTCAGTACTGGCTA	151.77329
BC37461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90277	WGS84	BC37461	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37461_Sspa_Oph2007_COI	NEWB11	1	BC37461_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37461	Sample	BC37461	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB11	COI	45	BC37461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB11	GAAGGCCTTCCGATGATC	151.77329
BC37461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90277	WGS84	BC37461	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37461_Upin_Bot2011_COI	NEWB11	1	BC37461_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37461	Sample	BC37461	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB11	COI	45	BC37461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB11	CTTTAATTACAGCGTTTTTATTGTTGT	151.77329
BC37462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90186	WGS84	BC37462	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37462_Mgig_Bot2012_COI	NEWB10	1	BC37462_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37462	Sample	BC37462	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB10	COI	45	BC37462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB10	TCTTATTCGTTGGAGACTTTATAACCCT	151.77113
BC37463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90467	WGS84	BC37463	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37463_Cmae_Bot2011_COI	NEWB9	1	BC37463_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37463	Sample	BC37463	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB9	COI	45	BC37463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB9	ATGAACAGTCTATCCTCCTTTAG	151.77235
BC37463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90467	WGS84	BC37463	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37463_Msal_Bot2012_COI	NEWB9	1	BC37463_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37463	Sample	BC37463	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB9	COI	45	BC37463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB9	GYTAGTTCCRATGATGTTAGCTG	151.77235
BC37465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91934	WGS84	BC37465	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37465_Aamu_Bax2006_COI	NEWB7	1	BC37465_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37465	Sample	BC37465	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB7	COI	45	BC37465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB7	GCACAACCGGGATCTTTACTTC	151.76933
BC37465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91934	WGS84	BC37465	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37465_Pcan_Bot2011_IGS	NEWB7	1	BC37465_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37465	Sample	BC37465	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB7	IGS	45	BC37465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB7	CGTAATCCTCAGTACTGGCTA	151.76933
BC37465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91934	WGS84	BC37465	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37465_Sspa_Oph2007_COI	NEWB7	1	BC37465_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37465	Sample	BC37465	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB7	COI	45	BC37465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB7	GAAGGCCTTCCGATGATC	151.76933
BC37465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91934	WGS84	BC37465	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37465_Upin_Bot2011_COI	NEWB7	1	BC37465_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37465	Sample	BC37465	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB7	COI	45	BC37465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB7	CTTTAATTACAGCGTTTTTATTGTTGT	151.76933
BC37466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92197	WGS84	BC37466	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37466_Mgig_Bot2012_COI	NEWB6	1	BC37466_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37466	Sample	BC37466	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB6	COI	45	BC37466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB6	TCTTATTCGTTGGAGACTTTATAACCCT	151.77168
BC37467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.9216	WGS84	BC37467	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37467_Cmae_Bot2011_COI	NEWB5	1	BC37467_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37467	Sample	BC37467	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB5	COI	45	BC37467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB5	ATGAACAGTCTATCCTCCTTTAG	151.76876
BC37467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.9216	WGS84	BC37467	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37467_Msal_Bot2012_COI	NEWB5	1	BC37467_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37467	Sample	BC37467	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB5	COI	45	BC37467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB5	GYTAGTTCCRATGATGTTAGCTG	151.76876
BC37469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92421	WGS84	BC37469	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37469_Aamu_Bax2006_COI	NEWB3	1	BC37469_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37469	Sample	BC37469	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB3	COI	45	BC37469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB3	GCACAACCGGGATCTTTACTTC	151.77064
BC37469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92421	WGS84	BC37469	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37469_Pcan_Bot2011_IGS	NEWB3	1	BC37469_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37469	Sample	BC37469	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB3	IGS	45	BC37469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB3	CGTAATCCTCAGTACTGGCTA	151.77064
BC37469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92421	WGS84	BC37469	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37469_Sspa_Oph2007_COI	NEWB3	1	BC37469_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37469	Sample	BC37469	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB3	COI	45	BC37469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB3	GAAGGCCTTCCGATGATC	151.77064
BC37469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92421	WGS84	BC37469	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37469_Upin_Bot2011_COI	NEWB3	1	BC37469_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37469	Sample	BC37469	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB3	COI	45	BC37469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB3	CTTTAATTACAGCGTTTTTATTGTTGT	151.77064
BC37470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92384	WGS84	BC37470	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37470_Cmae_Bot2011_COI	NEWB2	1	BC37470_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37470	Sample	BC37470	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB2	COI	45	BC37470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB2	ATGAACAGTCTATCCTCCTTTAG	151.76765
BC37470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92384	WGS84	BC37470	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37470_Msal_Bot2012_COI	NEWB2	1	BC37470_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37470	Sample	BC37470	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB2	COI	45	BC37470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB2	GYTAGTTCCRATGATGTTAGCTG	151.76765
BC37472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00231	WGS84	BC37472	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37472_Aamu_Bax2006_COI	BOTB1	1	BC37472_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37472	Sample	BC37472	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB1	COI	45	BC37472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB1	GCACAACCGGGATCTTTACTTC	151.20921
BC37472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00231	WGS84	BC37472	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37472_Pcan_Bot2011_IGS	BOTB1	1	BC37472_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37472	Sample	BC37472	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB1	IGS	45	BC37472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB1	CGTAATCCTCAGTACTGGCTA	151.20921
BC37472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00231	WGS84	BC37472	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37472_Sspa_Oph2007_COI	BOTB1	1	BC37472_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37472	Sample	BC37472	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB1	COI	45	BC37472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB1	GAAGGCCTTCCGATGATC	151.20921
BC37472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00231	WGS84	BC37472	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37472_Upin_Bot2011_COI	BOTB1	1	BC37472_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37472	Sample	BC37472	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB1	COI	45	BC37472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB1	CTTTAATTACAGCGTTTTTATTGTTGT	151.20921
BC37473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00212	WGS84	BC37473	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37473_Mgig_Bot2012_COI	BOTB2	1	BC37473_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37473	Sample	BC37473	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB2	COI	45	BC37473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB2	TCTTATTCGTTGGAGACTTTATAACCCT	151.21116
BC37474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00023	WGS84	BC37474	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37474_Cmae_Bot2011_COI	BOTB3	1	BC37474_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37474	Sample	BC37474	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB3	COI	45	BC37474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB3	ATGAACAGTCTATCCTCCTTTAG	151.20815
BC37474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00023	WGS84	BC37474	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37474_Msal_Bot2012_COI	BOTB3	1	BC37474_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37474	Sample	BC37474	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB3	COI	45	BC37474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB3	GYTAGTTCCRATGATGTTAGCTG	151.20815
BC37476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99864	WGS84	BC37476	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37476_Aamu_Bax2006_COI	BOTB5	1	BC37476_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37476	Sample	BC37476	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB5	COI	45	BC37476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB5	GCACAACCGGGATCTTTACTTC	151.20885
BC37476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99864	WGS84	BC37476	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37476_Pcan_Bot2011_IGS	BOTB5	1	BC37476_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37476	Sample	BC37476	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB5	IGS	45	BC37476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB5	CGTAATCCTCAGTACTGGCTA	151.20885
BC37476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99864	WGS84	BC37476	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37476_Sspa_Oph2007_COI	BOTB5	1	BC37476_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37476	Sample	BC37476	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB5	COI	45	BC37476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB5	GAAGGCCTTCCGATGATC	151.20885
BC37476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99864	WGS84	BC37476	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37476_Upin_Bot2011_COI	BOTB5	1	BC37476_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37476	Sample	BC37476	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB5	COI	45	BC37476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB5	CTTTAATTACAGCGTTTTTATTGTTGT	151.20885
BC37477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45332	WGS84	BC37477	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.984	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37477_Mgig_Bot2012_COI	KEMB35	1	BC37477_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37477	Sample	BC37477	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB35	COI	45	BC37477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB35	TCTTATTCGTTGGAGACTTTATAACCCT	150.89541
BC37478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45463	WGS84	BC37478	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37478_Cmae_Bot2011_COI	KEMB34	1	BC37478_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37478	Sample	BC37478	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB34	COI	45	BC37478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB34	ATGAACAGTCTATCCTCCTTTAG	150.89491
BC37478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45463	WGS84	BC37478	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37478_Msal_Bot2012_COI	KEMB34	1	BC37478_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37478	Sample	BC37478	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB34	COI	45	BC37478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB34	GYTAGTTCCRATGATGTTAGCTG	150.89491
BC37480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45578	WGS84	BC37480	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.132	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37480_Mgig_Bot2012_COI	KEMB32	1	BC37480_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37480	Sample	BC37480	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB32	COI	45	BC37480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB32	TCTTATTCGTTGGAGACTTTATAACCCT	150.89543
BC37481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45662	WGS84	BC37481	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37481_Cmae_Bot2011_COI	KEMB31	1	BC37481_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37481	Sample	BC37481	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB31	COI	45	BC37481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB31	ATGAACAGTCTATCCTCCTTTAG	150.89192
BC37481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45662	WGS84	BC37481	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37481_Msal_Bot2012_COI	KEMB31	1	BC37481_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37481	Sample	BC37481	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB31	COI	45	BC37481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB31	GYTAGTTCCRATGATGTTAGCTG	150.89192
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37483_Aamu_Bax2006_COI	KEMB29	1	BC37483_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	GCACAACCGGGATCTTTACTTC	150.89545
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37483_Pcan_Bot2011_IGS	KEMB29	1	BC37483_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB29	IGS	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	CGTAATCCTCAGTACTGGCTA	150.89545
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37483_Sspa_Oph2007_COI	KEMB29	1	BC37483_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	GAAGGCCTTCCGATGATC	150.89545
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37483_Upin_Bot2011_COI	KEMB29	1	BC37483_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	CTTTAATTACAGCGTTTTTATTGTTGT	150.89545
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37483_Vgib_Wil2025_COI	KEMB29	1	BC37483_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	CCCCTCTTTACAAGTTCTTTAGGGT	150.89545
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37484_Asen_Wil2025_COI	KEMB28	1	BC37484_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	GTTGTTGTTACAACACATGCTCTTTTG	150.89275
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.181	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37484_Mgig_Bot2012_COI	KEMB28	1	BC37484_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	TCTTATTCGTTGGAGACTTTATAACCCT	150.89275
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37485_Cmae_Bot2011_COI	KEMB27	1	BC37485_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	ATGAACAGTCTATCCTCCTTTAG	150.89094
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37485_Msal_Bot2012_COI	KEMB27	1	BC37485_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	GYTAGTTCCRATGATGTTAGCTG	150.89094
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37487_Aamu_Bax2006_COI	KEMB25	1	BC37487_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	GCACAACCGGGATCTTTACTTC	150.89433
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37487_Pcan_Bot2011_IGS	KEMB25	1	BC37487_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB25	IGS	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	CGTAATCCTCAGTACTGGCTA	150.89433
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37487_Sspa_Oph2007_COI	KEMB25	1	BC37487_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	GAAGGCCTTCCGATGATC	150.89433
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37487_Upin_Bot2011_COI	KEMB25	1	BC37487_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	CTTTAATTACAGCGTTTTTATTGTTGT	150.89433
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37487_Vgib_Wil2025_COI	KEMB25	1	BC37487_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	CCCCTCTTTACAAGTTCTTTAGGGT	150.89433
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37488_Asen_Wil2025_COI	KEMB24	1	BC37488_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	GTTGTTGTTACAACACATGCTCTTTTG	150.89227
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37488_Mgig_Bot2012_COI	KEMB24	1	BC37488_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	TCTTATTCGTTGGAGACTTTATAACCCT	150.89227
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37489_Cmae_Bot2011_COI	KEMB23	1	BC37489_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	ATGAACAGTCTATCCTCCTTTAG	150.89793
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37489_Msal_Bot2012_COI	KEMB23	1	BC37489_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	GYTAGTTCCRATGATGTTAGCTG	150.89793
BC37490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46159	WGS84	BC37490	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37490_Aamu_Bax2006_COI	KEMB22	1	BC37490_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37490	Sample	BC37490	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB22	COI	45	BC37490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB22	GCACAACCGGGATCTTTACTTC	150.89548
BC37490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46159	WGS84	BC37490	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37490_Pcan_Bot2011_IGS	KEMB22	1	BC37490_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37490	Sample	BC37490	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB22	IGS	45	BC37490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB22	CGTAATCCTCAGTACTGGCTA	150.89548
BC37490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46159	WGS84	BC37490	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37490_Sspa_Oph2007_COI	KEMB22	1	BC37490_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37490	Sample	BC37490	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB22	COI	45	BC37490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB22	GAAGGCCTTCCGATGATC	150.89548
BC37490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46159	WGS84	BC37490	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37490_Upin_Bot2011_COI	KEMB22	1	BC37490_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37490	Sample	BC37490	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB22	COI	45	BC37490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB22	CTTTAATTACAGCGTTTTTATTGTTGT	150.89548
BC37491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46158	WGS84	BC37491	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37491_Mgig_Bot2012_COI	KEMB21	1	BC37491_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37491	Sample	BC37491	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB21	COI	45	BC37491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB21	TCTTATTCGTTGGAGACTTTATAACCCT	150.89381
BC37492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46283	WGS84	BC37492	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37492_Cmae_Bot2011_COI	KEMB20	1	BC37492_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37492	Sample	BC37492	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB20	COI	45	BC37492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB20	ATGAACAGTCTATCCTCCTTTAG	150.89739
BC37492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46283	WGS84	BC37492	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37492_Msal_Bot2012_COI	KEMB20	1	BC37492_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37492	Sample	BC37492	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB20	COI	45	BC37492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB20	GYTAGTTCCRATGATGTTAGCTG	150.89739
BC37494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46358	WGS84	BC37494	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37494_Aamu_Bax2006_COI	KEMB18	1	BC37494_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37494	Sample	BC37494	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB18	COI	45	BC37494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB18	GCACAACCGGGATCTTTACTTC	150.90353
BC37494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46358	WGS84	BC37494	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37494_Pcan_Bot2011_IGS	KEMB18	1	BC37494_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37494	Sample	BC37494	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB18	IGS	45	BC37494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB18	CGTAATCCTCAGTACTGGCTA	150.90353
BC37494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46358	WGS84	BC37494	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37494_Sspa_Oph2007_COI	KEMB18	1	BC37494_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37494	Sample	BC37494	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB18	COI	45	BC37494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB18	GAAGGCCTTCCGATGATC	150.90353
BC37494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46358	WGS84	BC37494	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37494_Upin_Bot2011_COI	KEMB18	1	BC37494_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37494	Sample	BC37494	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB18	COI	45	BC37494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB18	CTTTAATTACAGCGTTTTTATTGTTGT	150.90353
BC37495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46364	WGS84	BC37495	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37495_Mgig_Bot2012_COI	KEMB17	1	BC37495_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37495	Sample	BC37495	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB17	COI	45	BC37495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB17	TCTTATTCGTTGGAGACTTTATAACCCT	150.90002
BC37496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46441	WGS84	BC37496	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37496_Cmae_Bot2011_COI	KEMB16	1	BC37496_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37496	Sample	BC37496	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB16	COI	45	BC37496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB16	ATGAACAGTCTATCCTCCTTTAG	150.9084
BC37496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46441	WGS84	BC37496	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37496_Msal_Bot2012_COI	KEMB16	1	BC37496_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37496	Sample	BC37496	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB16	COI	45	BC37496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB16	GYTAGTTCCRATGATGTTAGCTG	150.9084
BC37498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46521	WGS84	BC37498	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37498_Aamu_Bax2006_COI	KEMB14	1	BC37498_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37498	Sample	BC37498	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB14	COI	45	BC37498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB14	GCACAACCGGGATCTTTACTTC	150.90487
BC37498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46521	WGS84	BC37498	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37498_Pcan_Bot2011_IGS	KEMB14	1	BC37498_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37498	Sample	BC37498	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB14	IGS	45	BC37498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB14	CGTAATCCTCAGTACTGGCTA	150.90487
BC37498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46521	WGS84	BC37498	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37498_Sspa_Oph2007_COI	KEMB14	1	BC37498_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37498	Sample	BC37498	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB14	COI	45	BC37498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB14	GAAGGCCTTCCGATGATC	150.90487
BC37498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46521	WGS84	BC37498	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37498_Upin_Bot2011_COI	KEMB14	1	BC37498_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37498	Sample	BC37498	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB14	COI	45	BC37498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB14	CTTTAATTACAGCGTTTTTATTGTTGT	150.90487
BC37499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46502	WGS84	BC37499	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37499_Mgig_Bot2012_COI	KEMB13	1	BC37499_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37499	Sample	BC37499	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB13	COI	45	BC37499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB13	TCTTATTCGTTGGAGACTTTATAACCCT	150.90137
BC37500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46622	WGS84	BC37500	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37500_Cmae_Bot2011_COI	KEMB12	1	BC37500_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37500	Sample	BC37500	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB12	COI	45	BC37500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB12	ATGAACAGTCTATCCTCCTTTAG	150.90847
BC37500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46622	WGS84	BC37500	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37500_Msal_Bot2012_COI	KEMB12	1	BC37500_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37500	Sample	BC37500	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB12	COI	45	BC37500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB12	GYTAGTTCCRATGATGTTAGCTG	150.90847
BC37502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46689	WGS84	BC37502	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37502_Aamu_Bax2006_COI	KEMB10	1	BC37502_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37502	Sample	BC37502	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB10	COI	45	BC37502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB10	GCACAACCGGGATCTTTACTTC	150.90398
BC37502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46689	WGS84	BC37502	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37502_Pcan_Bot2011_IGS	KEMB10	1	BC37502_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37502	Sample	BC37502	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB10	IGS	45	BC37502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB10	CGTAATCCTCAGTACTGGCTA	150.90398
BC37502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46689	WGS84	BC37502	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37502_Sspa_Oph2007_COI	KEMB10	1	BC37502_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37502	Sample	BC37502	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB10	COI	45	BC37502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB10	GAAGGCCTTCCGATGATC	150.90398
BC37502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46689	WGS84	BC37502	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37502_Upin_Bot2011_COI	KEMB10	1	BC37502_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37502	Sample	BC37502	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB10	COI	45	BC37502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB10	CTTTAATTACAGCGTTTTTATTGTTGT	150.90398
BC37503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46663	WGS84	BC37503	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.965	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37503_Mgig_Bot2012_COI	KEMB9	1	BC37503_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37503	Sample	BC37503	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB9	COI	45	BC37503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB9	TCTTATTCGTTGGAGACTTTATAACCCT	150.90134
BC37504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46864	WGS84	BC37504	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37504_Cmae_Bot2011_COI	KEMB8	1	BC37504_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37504	Sample	BC37504	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB8	COI	45	BC37504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB8	ATGAACAGTCTATCCTCCTTTAG	150.90694
BC37504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46864	WGS84	BC37504	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37504_Msal_Bot2012_COI	KEMB8	1	BC37504_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37504	Sample	BC37504	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB8	COI	45	BC37504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB8	GYTAGTTCCRATGATGTTAGCTG	150.90694
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37506_Aamu_Bax2006_COI	KEMB6	1	BC37506_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	GCACAACCGGGATCTTTACTTC	150.90624
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37506_Pcan_Bot2011_IGS	KEMB6	1	BC37506_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB6	IGS	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	CGTAATCCTCAGTACTGGCTA	150.90624
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37506_Sspa_Oph2007_COI	KEMB6	1	BC37506_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	GAAGGCCTTCCGATGATC	150.90624
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37506_Upin_Bot2011_COI	KEMB6	1	BC37506_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	CTTTAATTACAGCGTTTTTATTGTTGT	150.90624
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37506_Vgib_Wil2025_COI	KEMB6	1	BC37506_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	CCCCTCTTTACAAGTTCTTTAGGGT	150.90624
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63716_Aamu_Bax2006_COI	FREB25	1	BC63716_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	GCACAACCGGGATCTTTACTTC	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63716_Pcan_Bot2011_IGS	FREB25	1	BC63716_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB25	IGS	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	CGTAATCCTCAGTACTGGCTA	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63716_Pper_Dia2013_COI	FREB25	1	BC63716_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Pper_Dia2013_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	CTTAGTGGCATTAATTCGDAATCC	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63716_Sspa_Oph2007_COI	FREB25	1	BC63716_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	GAAGGCCTTCCGATGATC	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63716_Upin_Bot2011_COI	FREB25	1	BC63716_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Upin_Bot2011_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	CTTTAATTACAGCGTTTTTATTGTTGT	115.74773
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63717_Cjap_Sim2018_COI	FREB20	1	BC63717_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63717_Mgig_Bot2012_COI	FREB20	1	BC63717_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	TCTTATTCGTTGGAGACTTTATAACCCT	115.74722
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63718_Cmae_Bot2011_COI	FREB15	1	BC63718_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	ATGAACAGTCTATCCTCCTTTAG	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63718_Msal_Bot2012_COI	FREB15	1	BC63718_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Msal_Bot2012_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	GYTAGTTCCRATGATGTTAGCTG	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63718_Pvir_Dia2013_COI	FREB15	1	BC63718_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	CTTAGTGGCATTAATTCGDAATCC	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63718_Rhar_Sim2018_COI	FREB15	1	BC63718_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	CTGCTGCTATTGCTCATG	115.74405
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63720_Cjap_Sim2018_COI	FREB5	1	BC63720_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63720_Mgig_Bot2012_COI	FREB5	1	BC63720_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	TCTTATTCGTTGGAGACTTTATAACCCT	115.74062
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63721_Cmae_Bot2011_COI	FREB33	1	BC63721_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	ATGAACAGTCTATCCTCCTTTAG	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63721_Msal_Bot2012_COI	FREB33	1	BC63721_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Msal_Bot2012_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	GYTAGTTCCRATGATGTTAGCTG	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63721_Pvir_Dia2013_COI	FREB33	1	BC63721_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	CTTAGTGGCATTAATTCGDAATCC	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63721_Rhar_Sim2018_COI	FREB33	1	BC63721_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	CTGCTGCTATTGCTCATG	115.75105
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63723_Aamu_Bax2006_COI	FREB31	1	BC63723_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	GCACAACCGGGATCTTTACTTC	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63723_Pcan_Bot2011_IGS	FREB31	1	BC63723_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB31	IGS	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	CGTAATCCTCAGTACTGGCTA	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63723_Pper_Dia2013_COI	FREB31	1	BC63723_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Pper_Dia2013_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	CTTAGTGGCATTAATTCGDAATCC	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63723_Sspa_Oph2007_COI	FREB31	1	BC63723_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	GAAGGCCTTCCGATGATC	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63723_Upin_Bot2011_COI	FREB31	1	BC63723_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Upin_Bot2011_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	CTTTAATTACAGCGTTTTTATTGTTGT	115.75126
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63724_Cjap_Sim2018_COI	FREB29	1	BC63724_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63724_Mgig_Bot2012_COI	FREB29	1	BC63724_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	TCTTATTCGTTGGAGACTTTATAACCCT	115.7501
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63725_Cmae_Bot2011_COI	FREB28	1	BC63725_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	ATGAACAGTCTATCCTCCTTTAG	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63725_Msal_Bot2012_COI	FREB28	1	BC63725_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Msal_Bot2012_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	GYTAGTTCCRATGATGTTAGCTG	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63725_Pvir_Dia2013_COI	FREB28	1	BC63725_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	CTTAGTGGCATTAATTCGDAATCC	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63725_Rhar_Sim2018_COI	FREB28	1	BC63725_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	CTGCTGCTATTGCTCATG	115.74897
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63727_Aamu_Bax2006_COI	FREB26	1	BC63727_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	GCACAACCGGGATCTTTACTTC	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63727_Pcan_Bot2011_IGS	FREB26	1	BC63727_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB26	IGS	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	CGTAATCCTCAGTACTGGCTA	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63727_Pper_Dia2013_COI	FREB26	1	BC63727_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Pper_Dia2013_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	CTTAGTGGCATTAATTCGDAATCC	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63727_Sspa_Oph2007_COI	FREB26	1	BC63727_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	GAAGGCCTTCCGATGATC	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63727_Upin_Bot2011_COI	FREB26	1	BC63727_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Upin_Bot2011_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	CTTTAATTACAGCGTTTTTATTGTTGT	115.74931
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63728_Cjap_Sim2018_COI	FREB24	1	BC63728_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63728_Mgig_Bot2012_COI	FREB24	1	BC63728_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	TCTTATTCGTTGGAGACTTTATAACCCT	115.7464
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63729_Cmae_Bot2011_COI	FREB23	1	BC63729_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	ATGAACAGTCTATCCTCCTTTAG	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63729_Msal_Bot2012_COI	FREB23	1	BC63729_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Msal_Bot2012_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	GYTAGTTCCRATGATGTTAGCTG	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63729_Pvir_Dia2013_COI	FREB23	1	BC63729_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	CTTAGTGGCATTAATTCGDAATCC	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63729_Rhar_Sim2018_COI	FREB23	1	BC63729_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	CTGCTGCTATTGCTCATG	115.74823
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63730_Aamu_Bax2006_COI	FREB22	1	BC63730_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	GCACAACCGGGATCTTTACTTC	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63730_Pcan_Bot2011_IGS	FREB22	1	BC63730_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB22	IGS	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	CGTAATCCTCAGTACTGGCTA	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63730_Pper_Dia2013_COI	FREB22	1	BC63730_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Pper_Dia2013_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	CTTAGTGGCATTAATTCGDAATCC	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63730_Sspa_Oph2007_COI	FREB22	1	BC63730_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	GAAGGCCTTCCGATGATC	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63730_Upin_Bot2011_COI	FREB22	1	BC63730_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Upin_Bot2011_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	CTTTAATTACAGCGTTTTTATTGTTGT	115.74684
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63731_Cjap_Sim2018_COI	FREB21	1	BC63731_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63731_Mgig_Bot2012_COI	FREB21	1	BC63731_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	TCTTATTCGTTGGAGACTTTATAACCCT	115.74527
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63732_Cmae_Bot2011_COI	FREB19	1	BC63732_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	ATGAACAGTCTATCCTCCTTTAG	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63732_Msal_Bot2012_COI	FREB19	1	BC63732_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Msal_Bot2012_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	GYTAGTTCCRATGATGTTAGCTG	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63732_Pvir_Dia2013_COI	FREB19	1	BC63732_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	CTTAGTGGCATTAATTCGDAATCC	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63732_Rhar_Sim2018_COI	FREB19	1	BC63732_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	CTGCTGCTATTGCTCATG	115.74577
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63734_Aamu_Bax2006_COI	FREB17	1	BC63734_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	GCACAACCGGGATCTTTACTTC	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63734_Pcan_Bot2011_IGS	FREB17	1	BC63734_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB17	IGS	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	CGTAATCCTCAGTACTGGCTA	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63734_Pper_Dia2013_COI	FREB17	1	BC63734_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Pper_Dia2013_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	CTTAGTGGCATTAATTCGDAATCC	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63734_Sspa_Oph2007_COI	FREB17	1	BC63734_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	GAAGGCCTTCCGATGATC	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63734_Upin_Bot2011_COI	FREB17	1	BC63734_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Upin_Bot2011_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	CTTTAATTACAGCGTTTTTATTGTTGT	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04686	WGS84	BC63734	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63734_Vgib_Wil2025_COI	FREB17	1	BC63734_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	CCCCTCTTTACAAGTTCTTTAGGGT	115.74626
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04642	WGS84	BC63735	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	27.321	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63735_Asen_Wil2025_COI	FREB16	1	BC63735_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Present	Sample	Asen_Wil2025_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	GTTGTTGTTACAACACATGCTCTTTTG	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63735_Cjap_Sim2018_COI	FREB16	1	BC63735_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63735_Mgig_Bot2012_COI	FREB16	1	BC63735_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	TCTTATTCGTTGGAGACTTTATAACCCT	115.7444
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63736_Cmae_Bot2011_COI	FREB14	1	BC63736_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	ATGAACAGTCTATCCTCCTTTAG	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63736_Msal_Bot2012_COI	FREB14	1	BC63736_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Msal_Bot2012_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	GYTAGTTCCRATGATGTTAGCTG	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63736_Pvir_Dia2013_COI	FREB14	1	BC63736_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	CTTAGTGGCATTAATTCGDAATCC	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63736_Rhar_Sim2018_COI	FREB14	1	BC63736_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	CTGCTGCTATTGCTCATG	115.74513
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63738_Aamu_Bax2006_COI	FREB12	1	BC63738_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	GCACAACCGGGATCTTTACTTC	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63738_Pcan_Bot2011_IGS	FREB12	1	BC63738_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB12	IGS	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	CGTAATCCTCAGTACTGGCTA	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63738_Pper_Dia2013_COI	FREB12	1	BC63738_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Pper_Dia2013_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	CTTAGTGGCATTAATTCGDAATCC	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63738_Sspa_Oph2007_COI	FREB12	1	BC63738_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	GAAGGCCTTCCGATGATC	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63738_Upin_Bot2011_COI	FREB12	1	BC63738_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Upin_Bot2011_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	CTTTAATTACAGCGTTTTTATTGTTGT	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04822	WGS84	BC63738	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63738_Vgib_Wil2025_COI	FREB12	1	BC63738_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	CCCCTCTTTACAAGTTCTTTAGGGT	115.74316
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04898	WGS84	BC63739	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	24.445	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63739_Asen_Wil2025_COI	FREB11	1	BC63739_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Present	Sample	Asen_Wil2025_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	GTTGTTGTTACAACACATGCTCTTTTG	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63739_Cjap_Sim2018_COI	FREB11	1	BC63739_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63739_Mgig_Bot2012_COI	FREB11	1	BC63739_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	TCTTATTCGTTGGAGACTTTATAACCCT	115.74423
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63741_Aamu_Bax2006_COI	FREB8	1	BC63741_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	GCACAACCGGGATCTTTACTTC	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63741_Pcan_Bot2011_IGS	FREB8	1	BC63741_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB8	IGS	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	CGTAATCCTCAGTACTGGCTA	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63741_Pper_Dia2013_COI	FREB8	1	BC63741_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Pper_Dia2013_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	CTTAGTGGCATTAATTCGDAATCC	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63741_Sspa_Oph2007_COI	FREB8	1	BC63741_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	GAAGGCCTTCCGATGATC	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63741_Upin_Bot2011_COI	FREB8	1	BC63741_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Upin_Bot2011_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	CTTTAATTACAGCGTTTTTATTGTTGT	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05009	WGS84	BC63741	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63741_Vgib_Wil2025_COI	FREB8	1	BC63741_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	CCCCTCTTTACAAGTTCTTTAGGGT	115.74305
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05009	WGS84	BC63742	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	24.544	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63742_Asen_Wil2025_COI	FREB7	1	BC63742_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Present	Sample	Asen_Wil2025_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	GTTGTTGTTACAACACATGCTCTTTTG	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63742_Cjap_Sim2018_COI	FREB7	1	BC63742_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63742_Mgig_Bot2012_COI	FREB7	1	BC63742_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	TCTTATTCGTTGGAGACTTTATAACCCT	115.74177
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63743_Cmae_Bot2011_COI	FREB6	1	BC63743_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	ATGAACAGTCTATCCTCCTTTAG	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63743_Msal_Bot2012_COI	FREB6	1	BC63743_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Msal_Bot2012_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	GYTAGTTCCRATGATGTTAGCTG	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63743_Pvir_Dia2013_COI	FREB6	1	BC63743_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	CTTAGTGGCATTAATTCGDAATCC	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63743_Rhar_Sim2018_COI	FREB6	1	BC63743_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	CTGCTGCTATTGCTCATG	115.74209
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63745_Aamu_Bax2006_COI	FREB3	1	BC63745_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	GCACAACCGGGATCTTTACTTC	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63745_Pcan_Bot2011_IGS	FREB3	1	BC63745_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB3	IGS	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	CGTAATCCTCAGTACTGGCTA	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63745_Pper_Dia2013_COI	FREB3	1	BC63745_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Pper_Dia2013_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	CTTAGTGGCATTAATTCGDAATCC	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63745_Sspa_Oph2007_COI	FREB3	1	BC63745_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	GAAGGCCTTCCGATGATC	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63745_Upin_Bot2011_COI	FREB3	1	BC63745_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Upin_Bot2011_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	CTTTAATTACAGCGTTTTTATTGTTGT	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05217	WGS84	BC63745	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63745_Vgib_Wil2025_COI	FREB3	1	BC63745_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	CCCCTCTTTACAAGTTCTTTAGGGT	115.73963
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05337	WGS84	BC63746	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	23.435	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63746_Asen_Wil2025_COI	FREB2	1	BC63746_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Present	Sample	Asen_Wil2025_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	GTTGTTGTTACAACACATGCTCTTTTG	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63746_Cjap_Sim2018_COI	FREB2	1	BC63746_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63746_Mgig_Bot2012_COI	FREB2	1	BC63746_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	TCTTATTCGTTGGAGACTTTATAACCCT	115.73868
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63747_Cmae_Bot2011_COI	FREB1	1	BC63747_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	ATGAACAGTCTATCCTCCTTTAG	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63747_Msal_Bot2012_COI	FREB1	1	BC63747_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Msal_Bot2012_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	GYTAGTTCCRATGATGTTAGCTG	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63747_Pvir_Dia2013_COI	FREB1	1	BC63747_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	CTTAGTGGCATTAATTCGDAATCC	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63747_Rhar_Sim2018_COI	FREB1	1	BC63747_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	CTGCTGCTATTGCTCATG	115.73746
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63749_Aamu_Bax2006_COI	KWIB55	1	BC63749_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	GCACAACCGGGATCTTTACTTC	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63749_Pcan_Bot2011_IGS	KWIB55	1	BC63749_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB55	IGS	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	CGTAATCCTCAGTACTGGCTA	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63749_Pper_Dia2013_COI	KWIB55	1	BC63749_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	CTTAGTGGCATTAATTCGDAATCC	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63749_Sspa_Oph2007_COI	KWIB55	1	BC63749_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	GAAGGCCTTCCGATGATC	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63749_Upin_Bot2011_COI	KWIB55	1	BC63749_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	CTTTAATTACAGCGTTTTTATTGTTGT	115.77353
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63750_Cmae_Bot2011_COI	KWIB50	1	BC63750_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	ATGAACAGTCTATCCTCCTTTAG	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63750_Msal_Bot2012_COI	KWIB50	1	BC63750_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	GYTAGTTCCRATGATGTTAGCTG	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63750_Pvir_Dia2013_COI	KWIB50	1	BC63750_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	CTTAGTGGCATTAATTCGDAATCC	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63750_Rhar_Sim2018_COI	KWIB50	1	BC63750_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	CTGCTGCTATTGCTCATG	115.76393
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63752_Aamu_Bax2006_COI	KWIB40	1	BC63752_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	GCACAACCGGGATCTTTACTTC	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63752_Pcan_Bot2011_IGS	KWIB40	1	BC63752_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB40	IGS	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	CGTAATCCTCAGTACTGGCTA	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63752_Pper_Dia2013_COI	KWIB40	1	BC63752_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	CTTAGTGGCATTAATTCGDAATCC	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63752_Sspa_Oph2007_COI	KWIB40	1	BC63752_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	GAAGGCCTTCCGATGATC	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63752_Upin_Bot2011_COI	KWIB40	1	BC63752_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	CTTTAATTACAGCGTTTTTATTGTTGT	115.7574
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63753_Cjap_Sim2018_COI	KWIB35	1	BC63753_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63753_Mgig_Bot2012_COI	KWIB35	1	BC63753_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	TCTTATTCGTTGGAGACTTTATAACCCT	115.75715
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63754_Cmae_Bot2011_COI	KWIB30	1	BC63754_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	ATGAACAGTCTATCCTCCTTTAG	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63754_Msal_Bot2012_COI	KWIB30	1	BC63754_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	GYTAGTTCCRATGATGTTAGCTG	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63754_Pvir_Dia2013_COI	KWIB30	1	BC63754_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	CTTAGTGGCATTAATTCGDAATCC	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63754_Rhar_Sim2018_COI	KWIB30	1	BC63754_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	CTGCTGCTATTGCTCATG	115.75607
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63756_Aamu_Bax2006_COI	KWIB20	1	BC63756_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	GCACAACCGGGATCTTTACTTC	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63756_Pcan_Bot2011_IGS	KWIB20	1	BC63756_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB20	IGS	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	CGTAATCCTCAGTACTGGCTA	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63756_Pper_Dia2013_COI	KWIB20	1	BC63756_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	CTTAGTGGCATTAATTCGDAATCC	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63756_Sspa_Oph2007_COI	KWIB20	1	BC63756_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	GAAGGCCTTCCGATGATC	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63756_Upin_Bot2011_COI	KWIB20	1	BC63756_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	CTTTAATTACAGCGTTTTTATTGTTGT	115.75447
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63757_Cjap_Sim2018_COI	KWIB15	1	BC63757_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63757_Mgig_Bot2012_COI	KWIB15	1	BC63757_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	TCTTATTCGTTGGAGACTTTATAACCCT	115.75598
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63758_Cmae_Bot2011_COI	KWIB10	1	BC63758_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	ATGAACAGTCTATCCTCCTTTAG	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63758_Msal_Bot2012_COI	KWIB10	1	BC63758_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	GYTAGTTCCRATGATGTTAGCTG	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63758_Pvir_Dia2013_COI	KWIB10	1	BC63758_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	CTTAGTGGCATTAATTCGDAATCC	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63758_Rhar_Sim2018_COI	KWIB10	1	BC63758_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	CTGCTGCTATTGCTCATG	115.74383
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63760_Cjap_Sim2018_COI	KWIB59	1	BC63760_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63760_Mgig_Bot2012_COI	KWIB59	1	BC63760_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	TCTTATTCGTTGGAGACTTTATAACCCT	115.77164
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63761_Cmae_Bot2011_COI	KWIB58	1	BC63761_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	ATGAACAGTCTATCCTCCTTTAG	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63761_Msal_Bot2012_COI	KWIB58	1	BC63761_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	GYTAGTTCCRATGATGTTAGCTG	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63761_Pvir_Dia2013_COI	KWIB58	1	BC63761_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	CTTAGTGGCATTAATTCGDAATCC	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63761_Rhar_Sim2018_COI	KWIB58	1	BC63761_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	CTGCTGCTATTGCTCATG	115.76952
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63763_Aamu_Bax2006_COI	KWIB56	1	BC63763_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	GCACAACCGGGATCTTTACTTC	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63763_Pcan_Bot2011_IGS	KWIB56	1	BC63763_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB56	IGS	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	CGTAATCCTCAGTACTGGCTA	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63763_Pper_Dia2013_COI	KWIB56	1	BC63763_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	CTTAGTGGCATTAATTCGDAATCC	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63763_Sspa_Oph2007_COI	KWIB56	1	BC63763_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	GAAGGCCTTCCGATGATC	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63763_Upin_Bot2011_COI	KWIB56	1	BC63763_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	CTTTAATTACAGCGTTTTTATTGTTGT	115.7711
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63764_Cjap_Sim2018_COI	KWIB54	1	BC63764_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63764_Mgig_Bot2012_COI	KWIB54	1	BC63764_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	TCTTATTCGTTGGAGACTTTATAACCCT	115.77258
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63765_Cmae_Bot2011_COI	KWIB53	1	BC63765_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	ATGAACAGTCTATCCTCCTTTAG	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63765_Msal_Bot2012_COI	KWIB53	1	BC63765_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	GYTAGTTCCRATGATGTTAGCTG	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63765_Pvir_Dia2013_COI	KWIB53	1	BC63765_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	CTTAGTGGCATTAATTCGDAATCC	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63765_Rhar_Sim2018_COI	KWIB53	1	BC63765_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	CTGCTGCTATTGCTCATG	115.77138
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63767_Aamu_Bax2006_COI	KWIB51	1	BC63767_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	GCACAACCGGGATCTTTACTTC	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63767_Pcan_Bot2011_IGS	KWIB51	1	BC63767_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB51	IGS	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	CGTAATCCTCAGTACTGGCTA	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63767_Pper_Dia2013_COI	KWIB51	1	BC63767_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	CTTAGTGGCATTAATTCGDAATCC	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63767_Sspa_Oph2007_COI	KWIB51	1	BC63767_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	GAAGGCCTTCCGATGATC	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63767_Upin_Bot2011_COI	KWIB51	1	BC63767_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	CTTTAATTACAGCGTTTTTATTGTTGT	115.76639
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63768_Cjap_Sim2018_COI	KWIB49	1	BC63768_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63768_Mgig_Bot2012_COI	KWIB49	1	BC63768_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	TCTTATTCGTTGGAGACTTTATAACCCT	115.76462
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63769_Cmae_Bot2011_COI	KWIB48	1	BC63769_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	ATGAACAGTCTATCCTCCTTTAG	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63769_Msal_Bot2012_COI	KWIB48	1	BC63769_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	GYTAGTTCCRATGATGTTAGCTG	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63769_Pvir_Dia2013_COI	KWIB48	1	BC63769_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	CTTAGTGGCATTAATTCGDAATCC	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63769_Rhar_Sim2018_COI	KWIB48	1	BC63769_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	CTGCTGCTATTGCTCATG	115.76106
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63770_Aamu_Bax2006_COI	KWIB47	1	BC63770_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	GCACAACCGGGATCTTTACTTC	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63770_Pcan_Bot2011_IGS	KWIB47	1	BC63770_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB47	IGS	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	CGTAATCCTCAGTACTGGCTA	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63770_Pper_Dia2013_COI	KWIB47	1	BC63770_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	CTTAGTGGCATTAATTCGDAATCC	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63770_Sspa_Oph2007_COI	KWIB47	1	BC63770_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	GAAGGCCTTCCGATGATC	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63770_Upin_Bot2011_COI	KWIB47	1	BC63770_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	CTTTAATTACAGCGTTTTTATTGTTGT	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21047	WGS84	BC63770	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63770_Vgib_Wil2025_COI	KWIB47	1	BC63770_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	CCCCTCTTTACAAGTTCTTTAGGGT	115.76457
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21083	WGS84	BC63771	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	32.113	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63771_Asen_Wil2025_COI	KWIB46	1	BC63771_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Present	Sample	Asen_Wil2025_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	GTTGTTGTTACAACACATGCTCTTTTG	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63771_Cjap_Sim2018_COI	KWIB46	1	BC63771_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63771_Mgig_Bot2012_COI	KWIB46	1	BC63771_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	TCTTATTCGTTGGAGACTTTATAACCCT	115.76322
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63772_Cmae_Bot2011_COI	KWIB44	1	BC63772_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	ATGAACAGTCTATCCTCCTTTAG	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63772_Msal_Bot2012_COI	KWIB44	1	BC63772_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	GYTAGTTCCRATGATGTTAGCTG	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63772_Pvir_Dia2013_COI	KWIB44	1	BC63772_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	CTTAGTGGCATTAATTCGDAATCC	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63772_Rhar_Sim2018_COI	KWIB44	1	BC63772_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	CTGCTGCTATTGCTCATG	115.76168
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63774_Aamu_Bax2006_COI	KWIB42	1	BC63774_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	GCACAACCGGGATCTTTACTTC	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63774_Pcan_Bot2011_IGS	KWIB42	1	BC63774_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB42	IGS	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	CGTAATCCTCAGTACTGGCTA	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63774_Pper_Dia2013_COI	KWIB42	1	BC63774_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	CTTAGTGGCATTAATTCGDAATCC	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63774_Sspa_Oph2007_COI	KWIB42	1	BC63774_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	GAAGGCCTTCCGATGATC	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63774_Upin_Bot2011_COI	KWIB42	1	BC63774_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	CTTTAATTACAGCGTTTTTATTGTTGT	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22943	WGS84	BC63774	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63774_Vgib_Wil2025_COI	KWIB42	1	BC63774_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	CCCCTCTTTACAAGTTCTTTAGGGT	115.75677
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22831	WGS84	BC63775	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.489	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63775_Asen_Wil2025_COI	KWIB41	1	BC63775_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Present	Sample	Asen_Wil2025_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	GTTGTTGTTACAACACATGCTCTTTTG	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63775_Cjap_Sim2018_COI	KWIB41	1	BC63775_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63775_Mgig_Bot2012_COI	KWIB41	1	BC63775_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	TCTTATTCGTTGGAGACTTTATAACCCT	115.75533
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63776_Cmae_Bot2011_COI	KWIB39	1	BC63776_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	ATGAACAGTCTATCCTCCTTTAG	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63776_Msal_Bot2012_COI	KWIB39	1	BC63776_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	GYTAGTTCCRATGATGTTAGCTG	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63776_Pvir_Dia2013_COI	KWIB39	1	BC63776_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	CTTAGTGGCATTAATTCGDAATCC	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63776_Rhar_Sim2018_COI	KWIB39	1	BC63776_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	CTGCTGCTATTGCTCATG	115.75608
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63778_Aamu_Bax2006_COI	KWIB37	1	BC63778_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	GCACAACCGGGATCTTTACTTC	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63778_Pcan_Bot2011_IGS	KWIB37	1	BC63778_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB37	IGS	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	CGTAATCCTCAGTACTGGCTA	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63778_Pper_Dia2013_COI	KWIB37	1	BC63778_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	CTTAGTGGCATTAATTCGDAATCC	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.528	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63778_Sspa_Oph2007_COI	KWIB37	1	BC63778_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	GAAGGCCTTCCGATGATC	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63778_Upin_Bot2011_COI	KWIB37	1	BC63778_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	CTTTAATTACAGCGTTTTTATTGTTGT	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23223	WGS84	BC63778	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63778_Vgib_Wil2025_COI	KWIB37	1	BC63778_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	CCCCTCTTTACAAGTTCTTTAGGGT	115.7565
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23195	WGS84	BC63779	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63779_Asen_Wil2025_COI	KWIB36	1	BC63779_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	GTTGTTGTTACAACACATGCTCTTTTG	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63779_Cjap_Sim2018_COI	KWIB36	1	BC63779_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63779_Mgig_Bot2012_COI	KWIB36	1	BC63779_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	TCTTATTCGTTGGAGACTTTATAACCCT	115.75378
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63781_Aamu_Bax2006_COI	KWIB33	1	BC63781_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	GCACAACCGGGATCTTTACTTC	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63781_Pcan_Bot2011_IGS	KWIB33	1	BC63781_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB33	IGS	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	CGTAATCCTCAGTACTGGCTA	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63781_Pper_Dia2013_COI	KWIB33	1	BC63781_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	CTTAGTGGCATTAATTCGDAATCC	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63781_Sspa_Oph2007_COI	KWIB33	1	BC63781_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	GAAGGCCTTCCGATGATC	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63781_Upin_Bot2011_COI	KWIB33	1	BC63781_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	CTTTAATTACAGCGTTTTTATTGTTGT	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23243	WGS84	BC63781	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63781_Vgib_Wil2025_COI	KWIB33	1	BC63781_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	CCCCTCTTTACAAGTTCTTTAGGGT	115.75438
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23397	WGS84	BC63782	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63782_Asen_Wil2025_COI	KWIB32	1	BC63782_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	GTTGTTGTTACAACACATGCTCTTTTG	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63782_Cjap_Sim2018_COI	KWIB32	1	BC63782_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63782_Mgig_Bot2012_COI	KWIB32	1	BC63782_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	TCTTATTCGTTGGAGACTTTATAACCCT	115.75315
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63783_Cmae_Bot2011_COI	KWIB31	1	BC63783_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	ATGAACAGTCTATCCTCCTTTAG	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63783_Msal_Bot2012_COI	KWIB31	1	BC63783_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	GYTAGTTCCRATGATGTTAGCTG	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63783_Pvir_Dia2013_COI	KWIB31	1	BC63783_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	CTTAGTGGCATTAATTCGDAATCC	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63783_Rhar_Sim2018_COI	KWIB31	1	BC63783_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	CTGCTGCTATTGCTCATG	115.75781
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63785_Aamu_Bax2006_COI	KWIB28	1	BC63785_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	GCACAACCGGGATCTTTACTTC	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63785_Pcan_Bot2011_IGS	KWIB28	1	BC63785_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB28	IGS	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	CGTAATCCTCAGTACTGGCTA	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63785_Pper_Dia2013_COI	KWIB28	1	BC63785_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	CTTAGTGGCATTAATTCGDAATCC	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.849	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63785_Sspa_Oph2007_COI	KWIB28	1	BC63785_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	GAAGGCCTTCCGATGATC	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63785_Upin_Bot2011_COI	KWIB28	1	BC63785_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	CTTTAATTACAGCGTTTTTATTGTTGT	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.2377	WGS84	BC63785	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63785_Vgib_Wil2025_COI	KWIB28	1	BC63785_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	CCCCTCTTTACAAGTTCTTTAGGGT	115.75654
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63786_Cjap_Sim2018_COI	KWIB27	1	BC63786_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63786_Mgig_Bot2012_COI	KWIB27	1	BC63786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	TCTTATTCGTTGGAGACTTTATAACCCT	115.75851
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63787_Cmae_Bot2011_COI	KWIB26	1	BC63787_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	ATGAACAGTCTATCCTCCTTTAG	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63787_Msal_Bot2012_COI	KWIB26	1	BC63787_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	GYTAGTTCCRATGATGTTAGCTG	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63787_Pvir_Dia2013_COI	KWIB26	1	BC63787_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	CTTAGTGGCATTAATTCGDAATCC	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63787_Rhar_Sim2018_COI	KWIB26	1	BC63787_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	CTGCTGCTATTGCTCATG	115.75575
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63789_Aamu_Bax2006_COI	KWIB23	1	BC63789_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	GCACAACCGGGATCTTTACTTC	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63789_Pcan_Bot2011_IGS	KWIB23	1	BC63789_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB23	IGS	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	CGTAATCCTCAGTACTGGCTA	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63789_Pper_Dia2013_COI	KWIB23	1	BC63789_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	CTTAGTGGCATTAATTCGDAATCC	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63789_Sspa_Oph2007_COI	KWIB23	1	BC63789_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	GAAGGCCTTCCGATGATC	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63789_Upin_Bot2011_COI	KWIB23	1	BC63789_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	CTTTAATTACAGCGTTTTTATTGTTGT	115.75448
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63790_Cmae_Bot2011_COI	KWIB22	1	BC63790_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	ATGAACAGTCTATCCTCCTTTAG	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63790_Msal_Bot2012_COI	KWIB22	1	BC63790_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	GYTAGTTCCRATGATGTTAGCTG	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63790_Pvir_Dia2013_COI	KWIB22	1	BC63790_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	CTTAGTGGCATTAATTCGDAATCC	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63790_Rhar_Sim2018_COI	KWIB22	1	BC63790_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	CTGCTGCTATTGCTCATG	115.75808
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63792_Aamu_Bax2006_COI	KWIB19	1	BC63792_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	GCACAACCGGGATCTTTACTTC	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63792_Pcan_Bot2011_IGS	KWIB19	1	BC63792_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB19	IGS	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	CGTAATCCTCAGTACTGGCTA	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63792_Pper_Dia2013_COI	KWIB19	1	BC63792_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	CTTAGTGGCATTAATTCGDAATCC	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.763	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63792_Sspa_Oph2007_COI	KWIB19	1	BC63792_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	GAAGGCCTTCCGATGATC	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63792_Upin_Bot2011_COI	KWIB19	1	BC63792_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	CTTTAATTACAGCGTTTTTATTGTTGT	115.75341
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63793_Cjap_Sim2018_COI	KWIB18	1	BC63793_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63793_Mgig_Bot2012_COI	KWIB18	1	BC63793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	TCTTATTCGTTGGAGACTTTATAACCCT	115.75699
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63794_Cmae_Bot2011_COI	KWIB17	1	BC63794_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	ATGAACAGTCTATCCTCCTTTAG	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63794_Msal_Bot2012_COI	KWIB17	1	BC63794_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	GYTAGTTCCRATGATGTTAGCTG	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63794_Pvir_Dia2013_COI	KWIB17	1	BC63794_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	CTTAGTGGCATTAATTCGDAATCC	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63794_Rhar_Sim2018_COI	KWIB17	1	BC63794_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	CTGCTGCTATTGCTCATG	115.75376
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63796_Aamu_Bax2006_COI	KWIB14	1	BC63796_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	GCACAACCGGGATCTTTACTTC	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63796_Pcan_Bot2011_IGS	KWIB14	1	BC63796_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB14	IGS	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	CGTAATCCTCAGTACTGGCTA	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63796_Pper_Dia2013_COI	KWIB14	1	BC63796_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	CTTAGTGGCATTAATTCGDAATCC	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.606	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63796_Sspa_Oph2007_COI	KWIB14	1	BC63796_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	GAAGGCCTTCCGATGATC	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63796_Upin_Bot2011_COI	KWIB14	1	BC63796_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	CTTTAATTACAGCGTTTTTATTGTTGT	115.7535
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63797_Cjap_Sim2018_COI	KWIB13	1	BC63797_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63797_Mgig_Bot2012_COI	KWIB13	1	BC63797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	TCTTATTCGTTGGAGACTTTATAACCCT	115.75209
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63798_Cmae_Bot2011_COI	KWIB12	1	BC63798_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	ATGAACAGTCTATCCTCCTTTAG	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63798_Msal_Bot2012_COI	KWIB12	1	BC63798_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	GYTAGTTCCRATGATGTTAGCTG	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63798_Pvir_Dia2013_COI	KWIB12	1	BC63798_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	CTTAGTGGCATTAATTCGDAATCC	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63798_Rhar_Sim2018_COI	KWIB12	1	BC63798_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	CTGCTGCTATTGCTCATG	115.75291
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63800_Aamu_Bax2006_COI	KWIB9	1	BC63800_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	GCACAACCGGGATCTTTACTTC	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63800_Pcan_Bot2011_IGS	KWIB9	1	BC63800_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB9	IGS	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	CGTAATCCTCAGTACTGGCTA	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63800_Pper_Dia2013_COI	KWIB9	1	BC63800_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	CTTAGTGGCATTAATTCGDAATCC	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63800_Sspa_Oph2007_COI	KWIB9	1	BC63800_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	GAAGGCCTTCCGATGATC	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63800_Upin_Bot2011_COI	KWIB9	1	BC63800_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	CTTTAATTACAGCGTTTTTATTGTTGT	115.74274
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63801_Cjap_Sim2018_COI	KWIB8	1	BC63801_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63801_Mgig_Bot2012_COI	KWIB8	1	BC63801_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	TCTTATTCGTTGGAGACTTTATAACCCT	115.74448
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63802_Cmae_Bot2011_COI	KWIB7	1	BC63802_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	ATGAACAGTCTATCCTCCTTTAG	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63802_Msal_Bot2012_COI	KWIB7	1	BC63802_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	GYTAGTTCCRATGATGTTAGCTG	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63802_Pvir_Dia2013_COI	KWIB7	1	BC63802_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	CTTAGTGGCATTAATTCGDAATCC	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63802_Rhar_Sim2018_COI	KWIB7	1	BC63802_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	CTGCTGCTATTGCTCATG	115.74276
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63804_Aamu_Bax2006_COI	KWIB4	1	BC63804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	GCACAACCGGGATCTTTACTTC	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63804_Pcan_Bot2011_IGS	KWIB4	1	BC63804_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB4	IGS	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	CGTAATCCTCAGTACTGGCTA	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63804_Pper_Dia2013_COI	KWIB4	1	BC63804_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	CTTAGTGGCATTAATTCGDAATCC	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63804_Sspa_Oph2007_COI	KWIB4	1	BC63804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	GAAGGCCTTCCGATGATC	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63804_Upin_Bot2011_COI	KWIB4	1	BC63804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	CTTTAATTACAGCGTTTTTATTGTTGT	115.74288
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63805_Cjap_Sim2018_COI	KWIB3	1	BC63805_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63805_Mgig_Bot2012_COI	KWIB3	1	BC63805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	TCTTATTCGTTGGAGACTTTATAACCCT	115.74123
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63806_Cmae_Bot2011_COI	KWIB2	1	BC63806_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	ATGAACAGTCTATCCTCCTTTAG	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63806_Msal_Bot2012_COI	KWIB2	1	BC63806_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	GYTAGTTCCRATGATGTTAGCTG	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63806_Pvir_Dia2013_COI	KWIB2	1	BC63806_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	CTTAGTGGCATTAATTCGDAATCC	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63806_Rhar_Sim2018_COI	KWIB2	1	BC63806_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	CTGCTGCTATTGCTCATG	115.74169
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63808_Aamu_Bax2006_COI	BUNB20	1	BC63808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	GCACAACCGGGATCTTTACTTC	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63808_Pcan_Bot2011_IGS	BUNB20	1	BC63808_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB20	IGS	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	CGTAATCCTCAGTACTGGCTA	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63808_Pper_Dia2013_COI	BUNB20	1	BC63808_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	CTTAGTGGCATTAATTCGDAATCC	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63808_Sspa_Oph2007_COI	BUNB20	1	BC63808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	GAAGGCCTTCCGATGATC	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63808_Upin_Bot2011_COI	BUNB20	1	BC63808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	CTTTAATTACAGCGTTTTTATTGTTGT	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.30696	WGS84	BC63808	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63808_Vgib_Wil2025_COI	BUNB20	1	BC63808_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	CCCCTCTTTACAAGTTCTTTAGGGT	115.64503
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63809_Cjap_Sim2018_COI	BUNB15	1	BC63809_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63809_Mgig_Bot2012_COI	BUNB15	1	BC63809_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	TCTTATTCGTTGGAGACTTTATAACCCT	115.66455
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63811_Aamu_Bax2006_COI	BUNB5	1	BC63811_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	GCACAACCGGGATCTTTACTTC	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63811_Pcan_Bot2011_IGS	BUNB5	1	BC63811_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB5	IGS	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	CGTAATCCTCAGTACTGGCTA	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63811_Pper_Dia2013_COI	BUNB5	1	BC63811_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	CTTAGTGGCATTAATTCGDAATCC	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63811_Sspa_Oph2007_COI	BUNB5	1	BC63811_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	GAAGGCCTTCCGATGATC	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63811_Upin_Bot2011_COI	BUNB5	1	BC63811_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	CTTTAATTACAGCGTTTTTATTGTTGT	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.32473	WGS84	BC63811	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63811_Vgib_Wil2025_COI	BUNB5	1	BC63811_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	CCCCTCTTTACAAGTTCTTTAGGGT	115.66712
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.30654	WGS84	BC63812	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63812_Asen_Wil2025_COI	BUNB23	1	BC63812_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	GTTGTTGTTACAACACATGCTCTTTTG	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63812_Cjap_Sim2018_COI	BUNB23	1	BC63812_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63812_Mgig_Bot2012_COI	BUNB23	1	BC63812_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	TCTTATTCGTTGGAGACTTTATAACCCT	115.64711
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63813_Cmae_Bot2011_COI	BUNB22	1	BC63813_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	ATGAACAGTCTATCCTCCTTTAG	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63813_Msal_Bot2012_COI	BUNB22	1	BC63813_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	GYTAGTTCCRATGATGTTAGCTG	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63813_Pvir_Dia2013_COI	BUNB22	1	BC63813_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	CTTAGTGGCATTAATTCGDAATCC	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63813_Rhar_Sim2018_COI	BUNB22	1	BC63813_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	CTGCTGCTATTGCTCATG	115.64537
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63815_Aamu_Bax2006_COI	BUNB19	1	BC63815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	GCACAACCGGGATCTTTACTTC	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63815_Pcan_Bot2011_IGS	BUNB19	1	BC63815_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB19	IGS	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	CGTAATCCTCAGTACTGGCTA	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63815_Pper_Dia2013_COI	BUNB19	1	BC63815_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	CTTAGTGGCATTAATTCGDAATCC	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63815_Sspa_Oph2007_COI	BUNB19	1	BC63815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	GAAGGCCTTCCGATGATC	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63815_Upin_Bot2011_COI	BUNB19	1	BC63815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	CTTTAATTACAGCGTTTTTATTGTTGT	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.30822	WGS84	BC63815	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63815_Vgib_Wil2025_COI	BUNB19	1	BC63815_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	CCCCTCTTTACAAGTTCTTTAGGGT	115.64399
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63816_Asen_Wil2025_COI	BUNB18	1	BC63816_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	GTTGTTGTTACAACACATGCTCTTTTG	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63816_Cjap_Sim2018_COI	BUNB18	1	BC63816_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63816_Mgig_Bot2012_COI	BUNB18	1	BC63816_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	TCTTATTCGTTGGAGACTTTATAACCCT	115.64277
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63817_Cmae_Bot2011_COI	BUNB17	1	BC63817_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	ATGAACAGTCTATCCTCCTTTAG	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63817_Msal_Bot2012_COI	BUNB17	1	BC63817_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	GYTAGTTCCRATGATGTTAGCTG	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63817_Pvir_Dia2013_COI	BUNB17	1	BC63817_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	CTTAGTGGCATTAATTCGDAATCC	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63817_Rhar_Sim2018_COI	BUNB17	1	BC63817_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	CTGCTGCTATTGCTCATG	115.66296
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63819_Aamu_Bax2006_COI	BUNB14	1	BC63819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	GCACAACCGGGATCTTTACTTC	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63819_Pcan_Bot2011_IGS	BUNB14	1	BC63819_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB14	IGS	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	CGTAATCCTCAGTACTGGCTA	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63819_Pper_Dia2013_COI	BUNB14	1	BC63819_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	CTTAGTGGCATTAATTCGDAATCC	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63819_Sspa_Oph2007_COI	BUNB14	1	BC63819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	GAAGGCCTTCCGATGATC	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63819_Upin_Bot2011_COI	BUNB14	1	BC63819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	CTTTAATTACAGCGTTTTTATTGTTGT	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63819_Vgib_Wil2025_COI	BUNB14	1	BC63819_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	CCCCTCTTTACAAGTTCTTTAGGGT	115.6627
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63820_Cmae_Bot2011_COI	BUNB13	1	BC63820_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	ATGAACAGTCTATCCTCCTTTAG	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63820_Msal_Bot2012_COI	BUNB13	1	BC63820_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	GYTAGTTCCRATGATGTTAGCTG	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63820_Pvir_Dia2013_COI	BUNB13	1	BC63820_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	CTTAGTGGCATTAATTCGDAATCC	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63820_Rhar_Sim2018_COI	BUNB13	1	BC63820_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	CTGCTGCTATTGCTCATG	115.66204
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63822_Aamu_Bax2006_COI	BUNB11	1	BC63822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	GCACAACCGGGATCTTTACTTC	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63822_Pcan_Bot2011_IGS	BUNB11	1	BC63822_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB11	IGS	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	CGTAATCCTCAGTACTGGCTA	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63822_Pper_Dia2013_COI	BUNB11	1	BC63822_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	CTTAGTGGCATTAATTCGDAATCC	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63822_Sspa_Oph2007_COI	BUNB11	1	BC63822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	GAAGGCCTTCCGATGATC	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63822_Upin_Bot2011_COI	BUNB11	1	BC63822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	CTTTAATTACAGCGTTTTTATTGTTGT	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63822_Vgib_Wil2025_COI	BUNB11	1	BC63822_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	CCCCTCTTTACAAGTTCTTTAGGGT	115.6586
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63823_Asen_Wil2025_COI	BUNB9	1	BC63823_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	GTTGTTGTTACAACACATGCTCTTTTG	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63823_Cjap_Sim2018_COI	BUNB9	1	BC63823_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63823_Mgig_Bot2012_COI	BUNB9	1	BC63823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	TCTTATTCGTTGGAGACTTTATAACCCT	115.66373
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63824_Cmae_Bot2011_COI	BUNB8	1	BC63824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	ATGAACAGTCTATCCTCCTTTAG	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63824_Msal_Bot2012_COI	BUNB8	1	BC63824_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	GYTAGTTCCRATGATGTTAGCTG	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63824_Pvir_Dia2013_COI	BUNB8	1	BC63824_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	CTTAGTGGCATTAATTCGDAATCC	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63824_Rhar_Sim2018_COI	BUNB8	1	BC63824_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	CTGCTGCTATTGCTCATG	115.66286
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63826_Aamu_Bax2006_COI	BUNB6	1	BC63826_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	GCACAACCGGGATCTTTACTTC	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63826_Pcan_Bot2011_IGS	BUNB6	1	BC63826_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB6	IGS	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	CGTAATCCTCAGTACTGGCTA	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63826_Pper_Dia2013_COI	BUNB6	1	BC63826_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	CTTAGTGGCATTAATTCGDAATCC	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63826_Sspa_Oph2007_COI	BUNB6	1	BC63826_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	GAAGGCCTTCCGATGATC	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63826_Upin_Bot2011_COI	BUNB6	1	BC63826_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	CTTTAATTACAGCGTTTTTATTGTTGT	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63826_Vgib_Wil2025_COI	BUNB6	1	BC63826_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	CCCCTCTTTACAAGTTCTTTAGGGT	115.66757
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63827_Cjap_Sim2018_COI	BUNB4	1	BC63827_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63827_Mgig_Bot2012_COI	BUNB4	1	BC63827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	TCTTATTCGTTGGAGACTTTATAACCCT	115.66528
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63828_Cmae_Bot2011_COI	BUNB3	1	BC63828_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	ATGAACAGTCTATCCTCCTTTAG	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63828_Msal_Bot2012_COI	BUNB3	1	BC63828_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	GYTAGTTCCRATGATGTTAGCTG	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63828_Pvir_Dia2013_COI	BUNB3	1	BC63828_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	CTTAGTGGCATTAATTCGDAATCC	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63828_Rhar_Sim2018_COI	BUNB3	1	BC63828_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	CTGCTGCTATTGCTCATG	115.66363
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63830_Asen_Wil2025_COI	BUNB1	1	BC63830_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	GTTGTTGTTACAACACATGCTCTTTTG	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63830_Cjap_Sim2018_COI	BUNB1	1	BC63830_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63830_Mgig_Bot2012_COI	BUNB1	1	BC63830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	TCTTATTCGTTGGAGACTTTATAACCCT	115.66652
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63831_Cmae_Bot2011_COI	GERB15	1	BC63831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	ATGAACAGTCTATCCTCCTTTAG	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63831_Msal_Bot2012_COI	GERB15	1	BC63831_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Msal_Bot2012_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	GYTAGTTCCRATGATGTTAGCTG	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63831_Pvir_Dia2013_COI	GERB15	1	BC63831_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	CTTAGTGGCATTAATTCGDAATCC	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63831_Rhar_Sim2018_COI	GERB15	1	BC63831_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	CTGCTGCTATTGCTCATG	114.60004
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63833_Aamu_Bax2006_COI	GERB5	1	BC63833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	GCACAACCGGGATCTTTACTTC	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63833_Pcan_Bot2011_IGS	GERB5	1	BC63833_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB5	IGS	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	CGTAATCCTCAGTACTGGCTA	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63833_Pper_Dia2013_COI	GERB5	1	BC63833_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Pper_Dia2013_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	CTTAGTGGCATTAATTCGDAATCC	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63833_Sspa_Oph2007_COI	GERB5	1	BC63833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	GAAGGCCTTCCGATGATC	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63833_Upin_Bot2011_COI	GERB5	1	BC63833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Upin_Bot2011_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	CTTTAATTACAGCGTTTTTATTGTTGT	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77534	WGS84	BC63833	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63833_Vgib_Wil2025_COI	GERB5	1	BC63833_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	CCCCTCTTTACAAGTTCTTTAGGGT	114.59844
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.7731	WGS84	BC63834	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63834_Asen_Wil2025_COI	GERB14	1	BC63834_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Asen_Wil2025_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	GTTGTTGTTACAACACATGCTCTTTTG	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63834_Cjap_Sim2018_COI	GERB14	1	BC63834_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63834_Mgig_Bot2012_COI	GERB14	1	BC63834_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	TCTTATTCGTTGGAGACTTTATAACCCT	114.60041
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63835_Cmae_Bot2011_COI	GERB13	1	BC63835_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	ATGAACAGTCTATCCTCCTTTAG	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63835_Msal_Bot2012_COI	GERB13	1	BC63835_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Msal_Bot2012_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	GYTAGTTCCRATGATGTTAGCTG	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63835_Pvir_Dia2013_COI	GERB13	1	BC63835_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	CTTAGTGGCATTAATTCGDAATCC	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63835_Rhar_Sim2018_COI	GERB13	1	BC63835_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	CTGCTGCTATTGCTCATG	114.59874
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63837_Aamu_Bax2006_COI	GERB11	1	BC63837_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	GCACAACCGGGATCTTTACTTC	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63837_Pcan_Bot2011_IGS	GERB11	1	BC63837_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB11	IGS	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	CGTAATCCTCAGTACTGGCTA	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63837_Pper_Dia2013_COI	GERB11	1	BC63837_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Pper_Dia2013_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	CTTAGTGGCATTAATTCGDAATCC	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63837_Sspa_Oph2007_COI	GERB11	1	BC63837_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	GAAGGCCTTCCGATGATC	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63837_Upin_Bot2011_COI	GERB11	1	BC63837_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Upin_Bot2011_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	CTTTAATTACAGCGTTTTTATTGTTGT	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77278	WGS84	BC63837	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63837_Vgib_Wil2025_COI	GERB11	1	BC63837_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	CCCCTCTTTACAAGTTCTTTAGGGT	114.59585
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.7742	WGS84	BC63838	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63838_Asen_Wil2025_COI	GERB9	1	BC63838_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Asen_Wil2025_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	GTTGTTGTTACAACACATGCTCTTTTG	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63838_Cjap_Sim2018_COI	GERB9	1	BC63838_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59939
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63839_Cmae_Bot2011_COI	GERB8	1	BC63839_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	ATGAACAGTCTATCCTCCTTTAG	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63839_Msal_Bot2012_COI	GERB8	1	BC63839_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Msal_Bot2012_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	GYTAGTTCCRATGATGTTAGCTG	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63839_Pvir_Dia2013_COI	GERB8	1	BC63839_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	CTTAGTGGCATTAATTCGDAATCC	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63839_Rhar_Sim2018_COI	GERB8	1	BC63839_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	CTGCTGCTATTGCTCATG	114.59782
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63840_Aamu_Bax2006_COI	GERB7	1	BC63840_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	GCACAACCGGGATCTTTACTTC	114.59635
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63840_Pcan_Bot2011_IGS	GERB7	1	BC63840_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB7	IGS	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	CGTAATCCTCAGTACTGGCTA	114.59635
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63840_Pper_Dia2013_COI	GERB7	1	BC63840_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Pper_Dia2013_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	CTTAGTGGCATTAATTCGDAATCC	114.59635
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63840_Sspa_Oph2007_COI	GERB7	1	BC63840_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	GAAGGCCTTCCGATGATC	114.59635
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63840_Upin_Bot2011_COI	GERB7	1	BC63840_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Upin_Bot2011_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	CTTTAATTACAGCGTTTTTATTGTTGT	114.59635
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63841_Cjap_Sim2018_COI	GERB6	1	BC63841_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63841_Mgig_Bot2012_COI	GERB6	1	BC63841_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	TCTTATTCGTTGGAGACTTTATAACCCT	114.59995
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63842_Cmae_Bot2011_COI	GERB4	1	BC63842_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	ATGAACAGTCTATCCTCCTTTAG	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63842_Msal_Bot2012_COI	GERB4	1	BC63842_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Msal_Bot2012_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	GYTAGTTCCRATGATGTTAGCTG	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63842_Pvir_Dia2013_COI	GERB4	1	BC63842_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	CTTAGTGGCATTAATTCGDAATCC	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63842_Rhar_Sim2018_COI	GERB4	1	BC63842_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	CTGCTGCTATTGCTCATG	114.59693
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63844_Aamu_Bax2006_COI	GERB2	1	BC63844_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	GCACAACCGGGATCTTTACTTC	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63844_Pcan_Bot2011_IGS	GERB2	1	BC63844_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB2	IGS	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	CGTAATCCTCAGTACTGGCTA	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63844_Pper_Dia2013_COI	GERB2	1	BC63844_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Pper_Dia2013_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	CTTAGTGGCATTAATTCGDAATCC	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63844_Sspa_Oph2007_COI	GERB2	1	BC63844_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	GAAGGCCTTCCGATGATC	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63844_Upin_Bot2011_COI	GERB2	1	BC63844_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Upin_Bot2011_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	CTTTAATTACAGCGTTTTTATTGTTGT	114.59591
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63845_Cjap_Sim2018_COI	KWIA60	1	BC63845_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63845_Mgig_Bot2012_COI	KWIA60	1	BC63845_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	TCTTATTCGTTGGAGACTTTATAACCCT	115.7731
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63846_Cmae_Bot2011_COI	KWIA59	1	BC63846_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	ATGAACAGTCTATCCTCCTTTAG	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63846_Msal_Bot2012_COI	KWIA59	1	BC63846_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	GYTAGTTCCRATGATGTTAGCTG	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63846_Pvir_Dia2013_COI	KWIA59	1	BC63846_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	CTTAGTGGCATTAATTCGDAATCC	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63846_Rhar_Sim2018_COI	KWIA59	1	BC63846_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	CTGCTGCTATTGCTCATG	115.77156
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63848_Aamu_Bax2006_COI	KWIA57	1	BC63848_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	GCACAACCGGGATCTTTACTTC	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63848_Pcan_Bot2011_IGS	KWIA57	1	BC63848_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA57	IGS	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	CGTAATCCTCAGTACTGGCTA	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63848_Pper_Dia2013_COI	KWIA57	1	BC63848_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	CTTAGTGGCATTAATTCGDAATCC	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63848_Sspa_Oph2007_COI	KWIA57	1	BC63848_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	GAAGGCCTTCCGATGATC	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63848_Upin_Bot2011_COI	KWIA57	1	BC63848_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	CTTTAATTACAGCGTTTTTATTGTTGT	115.77329
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63849_Cjap_Sim2018_COI	KWIA56	1	BC63849_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63849_Mgig_Bot2012_COI	KWIA56	1	BC63849_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	TCTTATTCGTTGGAGACTTTATAACCCT	115.77242
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63851_Aamu_Bax2006_COI	KWIA54	1	BC63851_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	GCACAACCGGGATCTTTACTTC	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63851_Pcan_Bot2011_IGS	KWIA54	1	BC63851_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA54	IGS	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	CGTAATCCTCAGTACTGGCTA	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63851_Pper_Dia2013_COI	KWIA54	1	BC63851_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	CTTAGTGGCATTAATTCGDAATCC	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63851_Sspa_Oph2007_COI	KWIA54	1	BC63851_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	GAAGGCCTTCCGATGATC	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63851_Upin_Bot2011_COI	KWIA54	1	BC63851_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	CTTTAATTACAGCGTTTTTATTGTTGT	115.7731
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63852_Cjap_Sim2018_COI	KWIA53	1	BC63852_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63852_Mgig_Bot2012_COI	KWIA53	1	BC63852_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	TCTTATTCGTTGGAGACTTTATAACCCT	115.77131
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63853_Cmae_Bot2011_COI	KWIA52	1	BC63853_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	ATGAACAGTCTATCCTCCTTTAG	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63853_Msal_Bot2012_COI	KWIA52	1	BC63853_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	GYTAGTTCCRATGATGTTAGCTG	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63853_Pvir_Dia2013_COI	KWIA52	1	BC63853_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	CTTAGTGGCATTAATTCGDAATCC	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63853_Rhar_Sim2018_COI	KWIA52	1	BC63853_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	CTGCTGCTATTGCTCATG	115.76936
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63855_Aamu_Bax2006_COI	KWIA50	1	BC63855_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	GCACAACCGGGATCTTTACTTC	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63855_Pcan_Bot2011_IGS	KWIA50	1	BC63855_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA50	IGS	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	CGTAATCCTCAGTACTGGCTA	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63855_Pper_Dia2013_COI	KWIA50	1	BC63855_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	CTTAGTGGCATTAATTCGDAATCC	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63855_Sspa_Oph2007_COI	KWIA50	1	BC63855_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	GAAGGCCTTCCGATGATC	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63855_Upin_Bot2011_COI	KWIA50	1	BC63855_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	CTTTAATTACAGCGTTTTTATTGTTGT	115.76389
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63856_Cjap_Sim2018_COI	KWIA49	1	BC63856_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63856_Mgig_Bot2012_COI	KWIA49	1	BC63856_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	TCTTATTCGTTGGAGACTTTATAACCCT	115.76479
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63857_Cmae_Bot2011_COI	KWIA48	1	BC63857_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	ATGAACAGTCTATCCTCCTTTAG	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63857_Msal_Bot2012_COI	KWIA48	1	BC63857_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	GYTAGTTCCRATGATGTTAGCTG	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63857_Pvir_Dia2013_COI	KWIA48	1	BC63857_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	CTTAGTGGCATTAATTCGDAATCC	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63857_Rhar_Sim2018_COI	KWIA48	1	BC63857_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	CTGCTGCTATTGCTCATG	115.76244
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63859_Aamu_Bax2006_COI	KWIA46	1	BC63859_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	GCACAACCGGGATCTTTACTTC	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63859_Pcan_Bot2011_IGS	KWIA46	1	BC63859_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA46	IGS	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	CGTAATCCTCAGTACTGGCTA	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63859_Pper_Dia2013_COI	KWIA46	1	BC63859_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	CTTAGTGGCATTAATTCGDAATCC	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63859_Sspa_Oph2007_COI	KWIA46	1	BC63859_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	GAAGGCCTTCCGATGATC	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63859_Upin_Bot2011_COI	KWIA46	1	BC63859_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	CTTTAATTACAGCGTTTTTATTGTTGT	115.7633
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63860_Cmae_Bot2011_COI	KWIA45	1	BC63860_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	ATGAACAGTCTATCCTCCTTTAG	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63860_Msal_Bot2012_COI	KWIA45	1	BC63860_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	GYTAGTTCCRATGATGTTAGCTG	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63860_Pvir_Dia2013_COI	KWIA45	1	BC63860_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	CTTAGTGGCATTAATTCGDAATCC	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63860_Rhar_Sim2018_COI	KWIA45	1	BC63860_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	CTGCTGCTATTGCTCATG	115.76077
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63862_Aamu_Bax2006_COI	KWIA43	1	BC63862_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	GCACAACCGGGATCTTTACTTC	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63862_Pcan_Bot2011_IGS	KWIA43	1	BC63862_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA43	IGS	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	CGTAATCCTCAGTACTGGCTA	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63862_Pper_Dia2013_COI	KWIA43	1	BC63862_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	CTTAGTGGCATTAATTCGDAATCC	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63862_Sspa_Oph2007_COI	KWIA43	1	BC63862_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	GAAGGCCTTCCGATGATC	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63862_Upin_Bot2011_COI	KWIA43	1	BC63862_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	CTTTAATTACAGCGTTTTTATTGTTGT	115.75889
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63863_Cjap_Sim2018_COI	KWIA42	1	BC63863_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63863_Mgig_Bot2012_COI	KWIA42	1	BC63863_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	TCTTATTCGTTGGAGACTTTATAACCCT	115.75685
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63864_Cmae_Bot2011_COI	KWIA41	1	BC63864_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	ATGAACAGTCTATCCTCCTTTAG	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63864_Msal_Bot2012_COI	KWIA41	1	BC63864_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	GYTAGTTCCRATGATGTTAGCTG	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63864_Pvir_Dia2013_COI	KWIA41	1	BC63864_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	CTTAGTGGCATTAATTCGDAATCC	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63864_Rhar_Sim2018_COI	KWIA41	1	BC63864_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	CTGCTGCTATTGCTCATG	115.75511
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63866_Aamu_Bax2006_COI	KWIA39	1	BC63866_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	GCACAACCGGGATCTTTACTTC	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63866_Pcan_Bot2011_IGS	KWIA39	1	BC63866_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA39	IGS	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	CGTAATCCTCAGTACTGGCTA	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63866_Pper_Dia2013_COI	KWIA39	1	BC63866_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	CTTAGTGGCATTAATTCGDAATCC	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63866_Sspa_Oph2007_COI	KWIA39	1	BC63866_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	GAAGGCCTTCCGATGATC	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63866_Upin_Bot2011_COI	KWIA39	1	BC63866_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	CTTTAATTACAGCGTTTTTATTGTTGT	115.75548
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63867_Cjap_Sim2018_COI	KWIA38	1	BC63867_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63867_Mgig_Bot2012_COI	KWIA38	1	BC63867_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	TCTTATTCGTTGGAGACTTTATAACCCT	115.75354
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63868_Cmae_Bot2011_COI	KWIA37	1	BC63868_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	ATGAACAGTCTATCCTCCTTTAG	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63868_Msal_Bot2012_COI	KWIA37	1	BC63868_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	GYTAGTTCCRATGATGTTAGCTG	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63868_Pvir_Dia2013_COI	KWIA37	1	BC63868_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	CTTAGTGGCATTAATTCGDAATCC	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63868_Rhar_Sim2018_COI	KWIA37	1	BC63868_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	CTGCTGCTATTGCTCATG	115.75622
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63870_Cjap_Sim2018_COI	KWIA35	1	BC63870_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63870_Mgig_Bot2012_COI	KWIA35	1	BC63870_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	TCTTATTCGTTGGAGACTTTATAACCCT	115.757
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63871_Cmae_Bot2011_COI	KWIA34	1	BC63871_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	ATGAACAGTCTATCCTCCTTTAG	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63871_Msal_Bot2012_COI	KWIA34	1	BC63871_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	GYTAGTTCCRATGATGTTAGCTG	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63871_Pvir_Dia2013_COI	KWIA34	1	BC63871_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	CTTAGTGGCATTAATTCGDAATCC	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63871_Rhar_Sim2018_COI	KWIA34	1	BC63871_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	CTGCTGCTATTGCTCATG	115.75462
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63873_Aamu_Bax2006_COI	KWIA32	1	BC63873_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	GCACAACCGGGATCTTTACTTC	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63873_Pcan_Bot2011_IGS	KWIA32	1	BC63873_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA32	IGS	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	CGTAATCCTCAGTACTGGCTA	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63873_Pper_Dia2013_COI	KWIA32	1	BC63873_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	CTTAGTGGCATTAATTCGDAATCC	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63873_Sspa_Oph2007_COI	KWIA32	1	BC63873_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	GAAGGCCTTCCGATGATC	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63873_Upin_Bot2011_COI	KWIA32	1	BC63873_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	CTTTAATTACAGCGTTTTTATTGTTGT	115.75404
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63874_Cjap_Sim2018_COI	KWIA31	1	BC63874_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63874_Mgig_Bot2012_COI	KWIA31	1	BC63874_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	TCTTATTCGTTGGAGACTTTATAACCCT	115.7577
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63875_Cmae_Bot2011_COI	KWIA30	1	BC63875_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	ATGAACAGTCTATCCTCCTTTAG	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63875_Msal_Bot2012_COI	KWIA30	1	BC63875_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	GYTAGTTCCRATGATGTTAGCTG	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63875_Pvir_Dia2013_COI	KWIA30	1	BC63875_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	CTTAGTGGCATTAATTCGDAATCC	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63875_Rhar_Sim2018_COI	KWIA30	1	BC63875_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	CTGCTGCTATTGCTCATG	115.75568
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63877_Aamu_Bax2006_COI	KWIA28	1	BC63877_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	GCACAACCGGGATCTTTACTTC	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63877_Pcan_Bot2011_IGS	KWIA28	1	BC63877_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA28	IGS	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	CGTAATCCTCAGTACTGGCTA	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63877_Pper_Dia2013_COI	KWIA28	1	BC63877_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	CTTAGTGGCATTAATTCGDAATCC	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63877_Sspa_Oph2007_COI	KWIA28	1	BC63877_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	GAAGGCCTTCCGATGATC	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63877_Upin_Bot2011_COI	KWIA28	1	BC63877_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	CTTTAATTACAGCGTTTTTATTGTTGT	115.75828
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63878_Cjap_Sim2018_COI	KWIA27	1	BC63878_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63878_Mgig_Bot2012_COI	KWIA27	1	BC63878_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	TCTTATTCGTTGGAGACTTTATAACCCT	115.7564
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63879_Cmae_Bot2011_COI	KWIA26	1	BC63879_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	ATGAACAGTCTATCCTCCTTTAG	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63879_Msal_Bot2012_COI	KWIA26	1	BC63879_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	GYTAGTTCCRATGATGTTAGCTG	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63879_Pvir_Dia2013_COI	KWIA26	1	BC63879_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	CTTAGTGGCATTAATTCGDAATCC	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63879_Rhar_Sim2018_COI	KWIA26	1	BC63879_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	CTGCTGCTATTGCTCATG	115.75454
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63880_Aamu_Bax2006_COI	KWIA25	1	BC63880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	GCACAACCGGGATCTTTACTTC	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63880_Pcan_Bot2011_IGS	KWIA25	1	BC63880_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA25	IGS	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	CGTAATCCTCAGTACTGGCTA	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63880_Pper_Dia2013_COI	KWIA25	1	BC63880_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	CTTAGTGGCATTAATTCGDAATCC	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63880_Sspa_Oph2007_COI	KWIA25	1	BC63880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	GAAGGCCTTCCGATGATC	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63880_Upin_Bot2011_COI	KWIA25	1	BC63880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	CTTTAATTACAGCGTTTTTATTGTTGT	115.75253
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63881_Cjap_Sim2018_COI	KWIA24	1	BC63881_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63881_Mgig_Bot2012_COI	KWIA24	1	BC63881_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	TCTTATTCGTTGGAGACTTTATAACCCT	115.75717
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63882_Cmae_Bot2011_COI	KWIA23	1	BC63882_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	ATGAACAGTCTATCCTCCTTTAG	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63882_Msal_Bot2012_COI	KWIA23	1	BC63882_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	GYTAGTTCCRATGATGTTAGCTG	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63882_Pvir_Dia2013_COI	KWIA23	1	BC63882_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	CTTAGTGGCATTAATTCGDAATCC	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63882_Rhar_Sim2018_COI	KWIA23	1	BC63882_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	CTGCTGCTATTGCTCATG	115.75331
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63884_Aamu_Bax2006_COI	KWIA21	1	BC63884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	GCACAACCGGGATCTTTACTTC	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63884_Pcan_Bot2011_IGS	KWIA21	1	BC63884_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA21	IGS	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	CGTAATCCTCAGTACTGGCTA	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63884_Pper_Dia2013_COI	KWIA21	1	BC63884_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	CTTAGTGGCATTAATTCGDAATCC	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63884_Sspa_Oph2007_COI	KWIA21	1	BC63884_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	GAAGGCCTTCCGATGATC	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63884_Upin_Bot2011_COI	KWIA21	1	BC63884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	CTTTAATTACAGCGTTTTTATTGTTGT	115.75653
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63885_Cjap_Sim2018_COI	KWIA20	1	BC63885_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63885_Mgig_Bot2012_COI	KWIA20	1	BC63885_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	TCTTATTCGTTGGAGACTTTATAACCCT	115.75401
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63886_Cmae_Bot2011_COI	KWIA19	1	BC63886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	ATGAACAGTCTATCCTCCTTTAG	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63886_Msal_Bot2012_COI	KWIA19	1	BC63886_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	GYTAGTTCCRATGATGTTAGCTG	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63886_Pvir_Dia2013_COI	KWIA19	1	BC63886_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	CTTAGTGGCATTAATTCGDAATCC	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63886_Rhar_Sim2018_COI	KWIA19	1	BC63886_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	CTGCTGCTATTGCTCATG	115.75228
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63888_Aamu_Bax2006_COI	KWIA17	1	BC63888_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	GCACAACCGGGATCTTTACTTC	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63888_Pcan_Bot2011_IGS	KWIA17	1	BC63888_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA17	IGS	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	CGTAATCCTCAGTACTGGCTA	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63888_Pper_Dia2013_COI	KWIA17	1	BC63888_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	CTTAGTGGCATTAATTCGDAATCC	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63888_Sspa_Oph2007_COI	KWIA17	1	BC63888_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	GAAGGCCTTCCGATGATC	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63888_Upin_Bot2011_COI	KWIA17	1	BC63888_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	CTTTAATTACAGCGTTTTTATTGTTGT	115.75289
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63889_Cjap_Sim2018_COI	KWIA16	1	BC63889_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63889_Mgig_Bot2012_COI	KWIA16	1	BC63889_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	TCTTATTCGTTGGAGACTTTATAACCCT	115.75755
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63891_Aamu_Bax2006_COI	KWIA14	1	BC63891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	GCACAACCGGGATCTTTACTTC	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63891_Pcan_Bot2011_IGS	KWIA14	1	BC63891_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA14	IGS	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	CGTAATCCTCAGTACTGGCTA	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63891_Pper_Dia2013_COI	KWIA14	1	BC63891_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	CTTAGTGGCATTAATTCGDAATCC	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63891_Sspa_Oph2007_COI	KWIA14	1	BC63891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	GAAGGCCTTCCGATGATC	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63891_Upin_Bot2011_COI	KWIA14	1	BC63891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	CTTTAATTACAGCGTTTTTATTGTTGT	115.75365
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63892_Cjap_Sim2018_COI	KWIA13	1	BC63892_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63892_Mgig_Bot2012_COI	KWIA13	1	BC63892_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	TCTTATTCGTTGGAGACTTTATAACCCT	115.75187
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63893_Cmae_Bot2011_COI	KWIA12	1	BC63893_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	ATGAACAGTCTATCCTCCTTTAG	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63893_Msal_Bot2012_COI	KWIA12	1	BC63893_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	GYTAGTTCCRATGATGTTAGCTG	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63893_Pvir_Dia2013_COI	KWIA12	1	BC63893_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	CTTAGTGGCATTAATTCGDAATCC	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63893_Rhar_Sim2018_COI	KWIA12	1	BC63893_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	CTGCTGCTATTGCTCATG	115.75246
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63895_Aamu_Bax2006_COI	KWIA10	1	BC63895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	GCACAACCGGGATCTTTACTTC	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63895_Pcan_Bot2011_IGS	KWIA10	1	BC63895_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA10	IGS	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	CGTAATCCTCAGTACTGGCTA	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63895_Pper_Dia2013_COI	KWIA10	1	BC63895_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	CTTAGTGGCATTAATTCGDAATCC	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63895_Sspa_Oph2007_COI	KWIA10	1	BC63895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	GAAGGCCTTCCGATGATC	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63895_Upin_Bot2011_COI	KWIA10	1	BC63895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	CTTTAATTACAGCGTTTTTATTGTTGT	115.74309
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63896_Cjap_Sim2018_COI	KWIA9	1	BC63896_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63896_Mgig_Bot2012_COI	KWIA9	1	BC63896_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	TCTTATTCGTTGGAGACTTTATAACCCT	115.74164
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63897_Cmae_Bot2011_COI	KWIA8	1	BC63897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	ATGAACAGTCTATCCTCCTTTAG	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63897_Msal_Bot2012_COI	KWIA8	1	BC63897_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	GYTAGTTCCRATGATGTTAGCTG	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63897_Pvir_Dia2013_COI	KWIA8	1	BC63897_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	CTTAGTGGCATTAATTCGDAATCC	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63897_Rhar_Sim2018_COI	KWIA8	1	BC63897_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	CTGCTGCTATTGCTCATG	115.74373
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63899_Aamu_Bax2006_COI	KWIA6	1	BC63899_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	GCACAACCGGGATCTTTACTTC	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63899_Pcan_Bot2011_IGS	KWIA6	1	BC63899_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA6	IGS	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	CGTAATCCTCAGTACTGGCTA	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63899_Pper_Dia2013_COI	KWIA6	1	BC63899_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	CTTAGTGGCATTAATTCGDAATCC	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63899_Sspa_Oph2007_COI	KWIA6	1	BC63899_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	GAAGGCCTTCCGATGATC	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63899_Upin_Bot2011_COI	KWIA6	1	BC63899_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	CTTTAATTACAGCGTTTTTATTGTTGT	115.74095
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63900_Cjap_Sim2018_COI	KWIA5	1	BC63900_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63900_Mgig_Bot2012_COI	KWIA5	1	BC63900_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	TCTTATTCGTTGGAGACTTTATAACCCT	115.74475
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63901_Cmae_Bot2011_COI	KWIA4	1	BC63901_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	ATGAACAGTCTATCCTCCTTTAG	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63901_Msal_Bot2012_COI	KWIA4	1	BC63901_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	GYTAGTTCCRATGATGTTAGCTG	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63901_Pvir_Dia2013_COI	KWIA4	1	BC63901_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	CTTAGTGGCATTAATTCGDAATCC	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63901_Rhar_Sim2018_COI	KWIA4	1	BC63901_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	CTGCTGCTATTGCTCATG	115.74282
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63903_Aamu_Bax2006_COI	KWIA2	1	BC63903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	GCACAACCGGGATCTTTACTTC	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63903_Pcan_Bot2011_IGS	KWIA2	1	BC63903_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA2	IGS	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	CGTAATCCTCAGTACTGGCTA	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63903_Pper_Dia2013_COI	KWIA2	1	BC63903_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	CTTAGTGGCATTAATTCGDAATCC	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63903_Sspa_Oph2007_COI	KWIA2	1	BC63903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	GAAGGCCTTCCGATGATC	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63903_Upin_Bot2011_COI	KWIA2	1	BC63903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	CTTTAATTACAGCGTTTTTATTGTTGT	115.74164
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63904_Cjap_Sim2018_COI	KWIA1	1	BC63904_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63904_Mgig_Bot2012_COI	KWIA1	1	BC63904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	TCTTATTCGTTGGAGACTTTATAACCCT	115.74031
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63905_Cmae_Bot2011_COI	FREA32	1	BC63905_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	ATGAACAGTCTATCCTCCTTTAG	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63905_Msal_Bot2012_COI	FREA32	1	BC63905_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Msal_Bot2012_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	GYTAGTTCCRATGATGTTAGCTG	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63905_Pvir_Dia2013_COI	FREA32	1	BC63905_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	CTTAGTGGCATTAATTCGDAATCC	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63905_Rhar_Sim2018_COI	FREA32	1	BC63905_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	CTGCTGCTATTGCTCATG	115.75126
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63907_Aamu_Bax2006_COI	FREA5	1	BC63907_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	GCACAACCGGGATCTTTACTTC	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63907_Pcan_Bot2011_IGS	FREA5	1	BC63907_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA5	IGS	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	CGTAATCCTCAGTACTGGCTA	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63907_Pper_Dia2013_COI	FREA5	1	BC63907_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Pper_Dia2013_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	CTTAGTGGCATTAATTCGDAATCC	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63907_Sspa_Oph2007_COI	FREA5	1	BC63907_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	GAAGGCCTTCCGATGATC	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63907_Upin_Bot2011_COI	FREA5	1	BC63907_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Upin_Bot2011_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	CTTTAATTACAGCGTTTTTATTGTTGT	115.74059
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63908_Cjap_Sim2018_COI	FREA10	1	BC63908_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63908_Esin_And2018_Cyb	FREA10	1	BC63908_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Esin_And2018_Cyb	FREA10	Cytb	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	ACCCCTCCTCATATCCAACCA	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63908_Mgig_Bot2012_COI	FREA10	1	BC63908_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	TCTTATTCGTTGGAGACTTTATAACCCT	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63908_Mjap_Gib2022_COI	FREA10	1	BC63908_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	AATTTAATTGTGACTAGGCATGGTCTG	115.74273
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63909_Cmae_Bot2011_COI	FREA15	1	BC63909_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	ATGAACAGTCTATCCTCCTTTAG	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63909_Hsan_Wil2021_COI	FREA15	1	BC63909_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	CCTGGGCCGGTATAGTAGGT	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63909_Mros_Gib2022_COI	FREA15	1	BC63909_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Mros_Gib2022_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63909_Msal_Bot2012_COI	FREA15	1	BC63909_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Msal_Bot2012_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	GYTAGTTCCRATGATGTTAGCTG	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63909_Pvir_Dia2013_COI	FREA15	1	BC63909_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	CTTAGTGGCATTAATTCGDAATCC	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63909_Rhar_Sim2018_COI	FREA15	1	BC63909_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	CTGCTGCTATTGCTCATG	115.74348
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63910_Aamu_Bax2006_COI	FREA20	1	BC63910_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	GCACAACCGGGATCTTTACTTC	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63910_Pcan_Bot2011_IGS	FREA20	1	BC63910_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA20	IGS	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	CGTAATCCTCAGTACTGGCTA	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63910_Pper_Dia2013_COI	FREA20	1	BC63910_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Pper_Dia2013_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	CTTAGTGGCATTAATTCGDAATCC	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63910_Sspa_Oph2007_COI	FREA20	1	BC63910_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	GAAGGCCTTCCGATGATC	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63910_Upin_Bot2011_COI	FREA20	1	BC63910_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Upin_Bot2011_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	CTTTAATTACAGCGTTTTTATTGTTGT	115.74761
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63911_Cjap_Sim2018_COI	FREA25	1	BC63911_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63911_Mgig_Bot2012_COI	FREA25	1	BC63911_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	TCTTATTCGTTGGAGACTTTATAACCCT	115.74809
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63912_Cmae_Bot2011_COI	FREA30	1	BC63912_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	ATGAACAGTCTATCCTCCTTTAG	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63912_Msal_Bot2012_COI	FREA30	1	BC63912_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Msal_Bot2012_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	GYTAGTTCCRATGATGTTAGCTG	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63912_Pvir_Dia2013_COI	FREA30	1	BC63912_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	CTTAGTGGCATTAATTCGDAATCC	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63912_Rhar_Sim2018_COI	FREA30	1	BC63912_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	CTGCTGCTATTGCTCATG	115.75018
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63914_Aamu_Bax2006_COI	FREA2	1	BC63914_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	GCACAACCGGGATCTTTACTTC	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63914_Pcan_Bot2011_IGS	FREA2	1	BC63914_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA2	IGS	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	CGTAATCCTCAGTACTGGCTA	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63914_Pper_Dia2013_COI	FREA2	1	BC63914_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Pper_Dia2013_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	CTTAGTGGCATTAATTCGDAATCC	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63914_Sspa_Oph2007_COI	FREA2	1	BC63914_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	GAAGGCCTTCCGATGATC	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63914_Upin_Bot2011_COI	FREA2	1	BC63914_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Upin_Bot2011_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	CTTTAATTACAGCGTTTTTATTGTTGT	115.73877
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63915_Cjap_Sim2018_COI	FREA3	1	BC63915_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63915_Esin_And2018_Cyb	FREA3	1	BC63915_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Esin_And2018_Cyb	FREA3	Cytb	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	ACCCCTCCTCATATCCAACCA	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63915_Mgig_Bot2012_COI	FREA3	1	BC63915_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	TCTTATTCGTTGGAGACTTTATAACCCT	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63915_Mjap_Gib2022_COI	FREA3	1	BC63915_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	AATTTAATTGTGACTAGGCATGGTCTG	115.73986
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63916_Cmae_Bot2011_COI	FREA4	1	BC63916_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	ATGAACAGTCTATCCTCCTTTAG	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63916_Hsan_Wil2021_COI	FREA4	1	BC63916_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	CCTGGGCCGGTATAGTAGGT	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63916_Mros_Gib2022_COI	FREA4	1	BC63916_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Mros_Gib2022_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63916_Msal_Bot2012_COI	FREA4	1	BC63916_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Msal_Bot2012_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	GYTAGTTCCRATGATGTTAGCTG	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63916_Pvir_Dia2013_COI	FREA4	1	BC63916_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	CTTAGTGGCATTAATTCGDAATCC	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63916_Rhar_Sim2018_COI	FREA4	1	BC63916_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	CTGCTGCTATTGCTCATG	115.74132
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63918_Aamu_Bax2006_COI	FREA7	1	BC63918_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	GCACAACCGGGATCTTTACTTC	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63918_Pcan_Bot2011_IGS	FREA7	1	BC63918_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA7	IGS	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	CGTAATCCTCAGTACTGGCTA	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63918_Pper_Dia2013_COI	FREA7	1	BC63918_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Pper_Dia2013_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	CTTAGTGGCATTAATTCGDAATCC	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63918_Sspa_Oph2007_COI	FREA7	1	BC63918_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	GAAGGCCTTCCGATGATC	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63918_Upin_Bot2011_COI	FREA7	1	BC63918_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Upin_Bot2011_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	CTTTAATTACAGCGTTTTTATTGTTGT	115.74189
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63919_Cjap_Sim2018_COI	FREA8	1	BC63919_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63919_Esin_And2018_Cyb	FREA8	1	BC63919_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Esin_And2018_Cyb	FREA8	Cytb	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	ACCCCTCCTCATATCCAACCA	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63919_Mgig_Bot2012_COI	FREA8	1	BC63919_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	TCTTATTCGTTGGAGACTTTATAACCCT	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63919_Mjap_Gib2022_COI	FREA8	1	BC63919_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	AATTTAATTGTGACTAGGCATGGTCTG	115.74336
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63921_Aamu_Bax2006_COI	FREA11	1	BC63921_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	GCACAACCGGGATCTTTACTTC	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63921_Pcan_Bot2011_IGS	FREA11	1	BC63921_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA11	IGS	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	CGTAATCCTCAGTACTGGCTA	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63921_Pper_Dia2013_COI	FREA11	1	BC63921_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Pper_Dia2013_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	CTTAGTGGCATTAATTCGDAATCC	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63921_Sspa_Oph2007_COI	FREA11	1	BC63921_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	GAAGGCCTTCCGATGATC	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63921_Upin_Bot2011_COI	FREA11	1	BC63921_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Upin_Bot2011_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	CTTTAATTACAGCGTTTTTATTGTTGT	115.74474
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63922_Cjap_Sim2018_COI	FREA12	1	BC63922_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63922_Esin_And2018_Cyb	FREA12	1	BC63922_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Esin_And2018_Cyb	FREA12	Cytb	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	ACCCCTCCTCATATCCAACCA	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63922_Mgig_Bot2012_COI	FREA12	1	BC63922_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	TCTTATTCGTTGGAGACTTTATAACCCT	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63922_Mjap_Gib2022_COI	FREA12	1	BC63922_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	AATTTAATTGTGACTAGGCATGGTCTG	115.74231
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63923_Cmae_Bot2011_COI	FREA13	1	BC63923_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	ATGAACAGTCTATCCTCCTTTAG	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63923_Hsan_Wil2021_COI	FREA13	1	BC63923_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	CCTGGGCCGGTATAGTAGGT	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63923_Mros_Gib2022_COI	FREA13	1	BC63923_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Mros_Gib2022_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63923_Msal_Bot2012_COI	FREA13	1	BC63923_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Msal_Bot2012_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	GYTAGTTCCRATGATGTTAGCTG	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63923_Pvir_Dia2013_COI	FREA13	1	BC63923_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	CTTAGTGGCATTAATTCGDAATCC	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63923_Rhar_Sim2018_COI	FREA13	1	BC63923_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	CTGCTGCTATTGCTCATG	115.7438
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63925_Aamu_Bax2006_COI	FREA16	1	BC63925_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	GCACAACCGGGATCTTTACTTC	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63925_Pcan_Bot2011_IGS	FREA16	1	BC63925_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA16	IGS	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	CGTAATCCTCAGTACTGGCTA	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63925_Pper_Dia2013_COI	FREA16	1	BC63925_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Pper_Dia2013_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	CTTAGTGGCATTAATTCGDAATCC	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63925_Sspa_Oph2007_COI	FREA16	1	BC63925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	GAAGGCCTTCCGATGATC	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63925_Upin_Bot2011_COI	FREA16	1	BC63925_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Upin_Bot2011_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	CTTTAATTACAGCGTTTTTATTGTTGT	115.74466
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63926_Cjap_Sim2018_COI	FREA21	1	BC63926_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63926_Esin_And2018_Cyb	FREA21	1	BC63926_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Esin_And2018_Cyb	FREA21	Cytb	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	ACCCCTCCTCATATCCAACCA	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63926_Mgig_Bot2012_COI	FREA21	1	BC63926_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	TCTTATTCGTTGGAGACTTTATAACCCT	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63926_Mjap_Gib2022_COI	FREA21	1	BC63926_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	AATTTAATTGTGACTAGGCATGGTCTG	115.74536
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63927_Cmae_Bot2011_COI	FREA18	1	BC63927_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	ATGAACAGTCTATCCTCCTTTAG	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63927_Hsan_Wil2021_COI	FREA18	1	BC63927_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	CCTGGGCCGGTATAGTAGGT	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63927_Mros_Gib2022_COI	FREA18	1	BC63927_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Mros_Gib2022_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63927_Msal_Bot2012_COI	FREA18	1	BC63927_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Msal_Bot2012_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	GYTAGTTCCRATGATGTTAGCTG	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63927_Pvir_Dia2013_COI	FREA18	1	BC63927_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	CTTAGTGGCATTAATTCGDAATCC	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63927_Rhar_Sim2018_COI	FREA18	1	BC63927_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	CTGCTGCTATTGCTCATG	115.74433
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63929_Aamu_Bax2006_COI	FREA17	1	BC63929_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	GCACAACCGGGATCTTTACTTC	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63929_Pcan_Bot2011_IGS	FREA17	1	BC63929_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA17	IGS	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	CGTAATCCTCAGTACTGGCTA	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63929_Pper_Dia2013_COI	FREA17	1	BC63929_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Pper_Dia2013_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	CTTAGTGGCATTAATTCGDAATCC	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63929_Sspa_Oph2007_COI	FREA17	1	BC63929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	GAAGGCCTTCCGATGATC	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63929_Upin_Bot2011_COI	FREA17	1	BC63929_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Upin_Bot2011_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	CTTTAATTACAGCGTTTTTATTGTTGT	115.74646
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63930_Cmae_Bot2011_COI	FREA22	1	BC63930_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	ATGAACAGTCTATCCTCCTTTAG	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63930_Hsan_Wil2021_COI	FREA22	1	BC63930_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	CCTGGGCCGGTATAGTAGGT	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63930_Mros_Gib2022_COI	FREA22	1	BC63930_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Mros_Gib2022_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63930_Msal_Bot2012_COI	FREA22	1	BC63930_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Msal_Bot2012_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	GYTAGTTCCRATGATGTTAGCTG	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63930_Pvir_Dia2013_COI	FREA22	1	BC63930_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	CTTAGTGGCATTAATTCGDAATCC	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63930_Rhar_Sim2018_COI	FREA22	1	BC63930_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	CTGCTGCTATTGCTCATG	115.74689
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63932_Aamu_Bax2006_COI	FREA24	1	BC63932_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	GCACAACCGGGATCTTTACTTC	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63932_Pcan_Bot2011_IGS	FREA24	1	BC63932_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA24	IGS	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	CGTAATCCTCAGTACTGGCTA	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63932_Pper_Dia2013_COI	FREA24	1	BC63932_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Pper_Dia2013_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	CTTAGTGGCATTAATTCGDAATCC	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63932_Sspa_Oph2007_COI	FREA24	1	BC63932_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	GAAGGCCTTCCGATGATC	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63932_Upin_Bot2011_COI	FREA24	1	BC63932_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Upin_Bot2011_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	CTTTAATTACAGCGTTTTTATTGTTGT	115.74651
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63933_Cjap_Sim2018_COI	FREA26	1	BC63933_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63933_Mgig_Bot2012_COI	FREA26	1	BC63933_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	TCTTATTCGTTGGAGACTTTATAACCCT	115.74984
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63934_Cmae_Bot2011_COI	FREA27	1	BC63934_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	ATGAACAGTCTATCCTCCTTTAG	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63934_Msal_Bot2012_COI	FREA27	1	BC63934_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Msal_Bot2012_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	GYTAGTTCCRATGATGTTAGCTG	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63934_Pvir_Dia2013_COI	FREA27	1	BC63934_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	CTTAGTGGCATTAATTCGDAATCC	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63934_Rhar_Sim2018_COI	FREA27	1	BC63934_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	CTGCTGCTATTGCTCATG	115.74776
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63936_Aamu_Bax2006_COI	FREA29	1	BC63936_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	GCACAACCGGGATCTTTACTTC	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63936_Pcan_Bot2011_IGS	FREA29	1	BC63936_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA29	IGS	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	CGTAATCCTCAGTACTGGCTA	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63936_Pper_Dia2013_COI	FREA29	1	BC63936_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Pper_Dia2013_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	CTTAGTGGCATTAATTCGDAATCC	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63936_Sspa_Oph2007_COI	FREA29	1	BC63936_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	GAAGGCCTTCCGATGATC	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63936_Upin_Bot2011_COI	FREA29	1	BC63936_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Upin_Bot2011_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	CTTTAATTACAGCGTTTTTATTGTTGT	115.75084
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63937_Cjap_Sim2018_COI	FREA31	1	BC63937_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63937_Mgig_Bot2012_COI	FREA31	1	BC63937_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	TCTTATTCGTTGGAGACTTTATAACCCT	115.75211
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63938_Cmae_Bot2011_COI	BUNA23	1	BC63938_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	ATGAACAGTCTATCCTCCTTTAG	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63938_Hsan_Wil2021_COI	BUNA23	1	BC63938_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	CCTGGGCCGGTATAGTAGGT	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63938_Mros_Gib2022_COI	BUNA23	1	BC63938_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63938_Msal_Bot2012_COI	BUNA23	1	BC63938_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	GYTAGTTCCRATGATGTTAGCTG	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63938_Pvir_Dia2013_COI	BUNA23	1	BC63938_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	CTTAGTGGCATTAATTCGDAATCC	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63938_Rhar_Sim2018_COI	BUNA23	1	BC63938_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	CTGCTGCTATTGCTCATG	115.64785
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63940_Asen_Wil2025_COI	BUNA21	1	BC63940_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	GTTGTTGTTACAACACATGCTCTTTTG	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63940_Cjap_Sim2018_COI	BUNA21	1	BC63940_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63940_Esin_And2018_Cyb	BUNA21	1	BC63940_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA21	Cytb	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	ACCCCTCCTCATATCCAACCA	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63940_Mgig_Bot2012_COI	BUNA21	1	BC63940_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	TCTTATTCGTTGGAGACTTTATAACCCT	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63940_Mjap_Gib2022_COI	BUNA21	1	BC63940_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	AATTTAATTGTGACTAGGCATGGTCTG	115.64669
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63941_Cmae_Bot2011_COI	BUNA20	1	BC63941_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	ATGAACAGTCTATCCTCCTTTAG	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63941_Hsan_Wil2021_COI	BUNA20	1	BC63941_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	CCTGGGCCGGTATAGTAGGT	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63941_Mros_Gib2022_COI	BUNA20	1	BC63941_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63941_Msal_Bot2012_COI	BUNA20	1	BC63941_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	GYTAGTTCCRATGATGTTAGCTG	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63941_Pvir_Dia2013_COI	BUNA20	1	BC63941_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	CTTAGTGGCATTAATTCGDAATCC	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63941_Rhar_Sim2018_COI	BUNA20	1	BC63941_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	CTGCTGCTATTGCTCATG	115.64524
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63943_Aamu_Bax2006_COI	BUNA18	1	BC63943_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	GCACAACCGGGATCTTTACTTC	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63943_Pcan_Bot2011_IGS	BUNA18	1	BC63943_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA18	IGS	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	CGTAATCCTCAGTACTGGCTA	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63943_Pper_Dia2013_COI	BUNA18	1	BC63943_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	CTTAGTGGCATTAATTCGDAATCC	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63943_Sspa_Oph2007_COI	BUNA18	1	BC63943_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	GAAGGCCTTCCGATGATC	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63943_Upin_Bot2011_COI	BUNA18	1	BC63943_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	CTTTAATTACAGCGTTTTTATTGTTGT	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63943_Vgib_Wil2025_COI	BUNA18	1	BC63943_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	CCCCTCTTTACAAGTTCTTTAGGGT	115.64241
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63944_Asen_Wil2025_COI	BUNA17	1	BC63944_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	GTTGTTGTTACAACACATGCTCTTTTG	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63944_Cjap_Sim2018_COI	BUNA17	1	BC63944_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63944_Esin_And2018_Cyb	BUNA17	1	BC63944_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA17	Cytb	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	ACCCCTCCTCATATCCAACCA	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63944_Mgig_Bot2012_COI	BUNA17	1	BC63944_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	TCTTATTCGTTGGAGACTTTATAACCCT	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63944_Mjap_Gib2022_COI	BUNA17	1	BC63944_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	AATTTAATTGTGACTAGGCATGGTCTG	115.66307
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63945_Cmae_Bot2011_COI	BUNA16	1	BC63945_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	ATGAACAGTCTATCCTCCTTTAG	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63945_Hsan_Wil2021_COI	BUNA16	1	BC63945_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	CCTGGGCCGGTATAGTAGGT	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63945_Mros_Gib2022_COI	BUNA16	1	BC63945_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63945_Msal_Bot2012_COI	BUNA16	1	BC63945_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	GYTAGTTCCRATGATGTTAGCTG	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63945_Pvir_Dia2013_COI	BUNA16	1	BC63945_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	CTTAGTGGCATTAATTCGDAATCC	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63945_Rhar_Sim2018_COI	BUNA16	1	BC63945_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	CTGCTGCTATTGCTCATG	115.66118
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63947_Aamu_Bax2006_COI	BUNA14	1	BC63947_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	GCACAACCGGGATCTTTACTTC	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63947_Pcan_Bot2011_IGS	BUNA14	1	BC63947_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA14	IGS	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	CGTAATCCTCAGTACTGGCTA	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63947_Pper_Dia2013_COI	BUNA14	1	BC63947_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	CTTAGTGGCATTAATTCGDAATCC	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63947_Sspa_Oph2007_COI	BUNA14	1	BC63947_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	GAAGGCCTTCCGATGATC	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63947_Upin_Bot2011_COI	BUNA14	1	BC63947_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	CTTTAATTACAGCGTTTTTATTGTTGT	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63947_Vgib_Wil2025_COI	BUNA14	1	BC63947_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	CCCCTCTTTACAAGTTCTTTAGGGT	115.66383
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63948_Asen_Wil2025_COI	BUNA13	1	BC63948_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	GTTGTTGTTACAACACATGCTCTTTTG	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63948_Cjap_Sim2018_COI	BUNA13	1	BC63948_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63948_Esin_And2018_Cyb	BUNA13	1	BC63948_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA13	Cytb	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	ACCCCTCCTCATATCCAACCA	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63948_Mgig_Bot2012_COI	BUNA13	1	BC63948_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	TCTTATTCGTTGGAGACTTTATAACCCT	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63948_Mjap_Gib2022_COI	BUNA13	1	BC63948_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	AATTTAATTGTGACTAGGCATGGTCTG	115.66214
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63949_Cmae_Bot2011_COI	BUNA12	1	BC63949_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	ATGAACAGTCTATCCTCCTTTAG	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63949_Hsan_Wil2021_COI	BUNA12	1	BC63949_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	CCTGGGCCGGTATAGTAGGT	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63949_Mros_Gib2022_COI	BUNA12	1	BC63949_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63949_Msal_Bot2012_COI	BUNA12	1	BC63949_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	GYTAGTTCCRATGATGTTAGCTG	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63949_Pvir_Dia2013_COI	BUNA12	1	BC63949_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	CTTAGTGGCATTAATTCGDAATCC	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63949_Rhar_Sim2018_COI	BUNA12	1	BC63949_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	CTGCTGCTATTGCTCATG	115.66024
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63950_Aamu_Bax2006_COI	BUNA11	1	BC63950_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	GCACAACCGGGATCTTTACTTC	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63950_Pcan_Bot2011_IGS	BUNA11	1	BC63950_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA11	IGS	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	CGTAATCCTCAGTACTGGCTA	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63950_Pper_Dia2013_COI	BUNA11	1	BC63950_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	CTTAGTGGCATTAATTCGDAATCC	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63950_Sspa_Oph2007_COI	BUNA11	1	BC63950_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	GAAGGCCTTCCGATGATC	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63950_Upin_Bot2011_COI	BUNA11	1	BC63950_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	CTTTAATTACAGCGTTTTTATTGTTGT	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63950_Vgib_Wil2025_COI	BUNA11	1	BC63950_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	CCCCTCTTTACAAGTTCTTTAGGGT	115.65884
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63951_Asen_Wil2025_COI	BUNA10	1	BC63951_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	GTTGTTGTTACAACACATGCTCTTTTG	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63951_Cjap_Sim2018_COI	BUNA10	1	BC63951_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63951_Esin_And2018_Cyb	BUNA10	1	BC63951_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA10	Cytb	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	ACCCCTCCTCATATCCAACCA	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63951_Mgig_Bot2012_COI	BUNA10	1	BC63951_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	TCTTATTCGTTGGAGACTTTATAACCCT	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63951_Mjap_Gib2022_COI	BUNA10	1	BC63951_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	AATTTAATTGTGACTAGGCATGGTCTG	115.66622
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63952_Cmae_Bot2011_COI	BUNA9	1	BC63952_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	ATGAACAGTCTATCCTCCTTTAG	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63952_Hsan_Wil2021_COI	BUNA9	1	BC63952_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	CCTGGGCCGGTATAGTAGGT	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63952_Mros_Gib2022_COI	BUNA9	1	BC63952_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63952_Msal_Bot2012_COI	BUNA9	1	BC63952_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	GYTAGTTCCRATGATGTTAGCTG	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63952_Pvir_Dia2013_COI	BUNA9	1	BC63952_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	CTTAGTGGCATTAATTCGDAATCC	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63952_Rhar_Sim2018_COI	BUNA9	1	BC63952_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	CTGCTGCTATTGCTCATG	115.66476
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63954_Aamu_Bax2006_COI	BUNA7	1	BC63954_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	GCACAACCGGGATCTTTACTTC	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63954_Pcan_Bot2011_IGS	BUNA7	1	BC63954_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA7	IGS	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	CGTAATCCTCAGTACTGGCTA	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63954_Pper_Dia2013_COI	BUNA7	1	BC63954_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	CTTAGTGGCATTAATTCGDAATCC	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63954_Sspa_Oph2007_COI	BUNA7	1	BC63954_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	GAAGGCCTTCCGATGATC	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63954_Upin_Bot2011_COI	BUNA7	1	BC63954_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	CTTTAATTACAGCGTTTTTATTGTTGT	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63954_Vgib_Wil2025_COI	BUNA7	1	BC63954_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	CCCCTCTTTACAAGTTCTTTAGGGT	115.66124
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63955_Asen_Wil2025_COI	BUNA6	1	BC63955_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	GTTGTTGTTACAACACATGCTCTTTTG	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63955_Cjap_Sim2018_COI	BUNA6	1	BC63955_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63955_Esin_And2018_Cyb	BUNA6	1	BC63955_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA6	Cytb	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	ACCCCTCCTCATATCCAACCA	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63955_Mgig_Bot2012_COI	BUNA6	1	BC63955_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	TCTTATTCGTTGGAGACTTTATAACCCT	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63955_Mjap_Gib2022_COI	BUNA6	1	BC63955_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	AATTTAATTGTGACTAGGCATGGTCTG	115.6689
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63956_Cmae_Bot2011_COI	BUNA5	1	BC63956_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	ATGAACAGTCTATCCTCCTTTAG	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63956_Hsan_Wil2021_COI	BUNA5	1	BC63956_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	CCTGGGCCGGTATAGTAGGT	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63956_Mros_Gib2022_COI	BUNA5	1	BC63956_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63956_Msal_Bot2012_COI	BUNA5	1	BC63956_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	GYTAGTTCCRATGATGTTAGCTG	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63956_Pvir_Dia2013_COI	BUNA5	1	BC63956_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	CTTAGTGGCATTAATTCGDAATCC	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63956_Rhar_Sim2018_COI	BUNA5	1	BC63956_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	CTGCTGCTATTGCTCATG	115.66727
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63958_Aamu_Bax2006_COI	BUNA3	1	BC63958_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	GCACAACCGGGATCTTTACTTC	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63958_Pcan_Bot2011_IGS	BUNA3	1	BC63958_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA3	IGS	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	CGTAATCCTCAGTACTGGCTA	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63958_Pper_Dia2013_COI	BUNA3	1	BC63958_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	CTTAGTGGCATTAATTCGDAATCC	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63958_Sspa_Oph2007_COI	BUNA3	1	BC63958_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	GAAGGCCTTCCGATGATC	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63958_Upin_Bot2011_COI	BUNA3	1	BC63958_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	CTTTAATTACAGCGTTTTTATTGTTGT	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63958_Vgib_Wil2025_COI	BUNA3	1	BC63958_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	CCCCTCTTTACAAGTTCTTTAGGGT	115.66343
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63959_Asen_Wil2025_COI	BUNA2	1	BC63959_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	GTTGTTGTTACAACACATGCTCTTTTG	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63959_Cjap_Sim2018_COI	BUNA2	1	BC63959_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63959_Esin_And2018_Cyb	BUNA2	1	BC63959_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA2	Cytb	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	ACCCCTCCTCATATCCAACCA	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63959_Mgig_Bot2012_COI	BUNA2	1	BC63959_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	TCTTATTCGTTGGAGACTTTATAACCCT	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63959_Mjap_Gib2022_COI	BUNA2	1	BC63959_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	AATTTAATTGTGACTAGGCATGGTCTG	115.66781
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63961_Aamu_Bax2006_COI	GERA15	1	BC63961_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	GCACAACCGGGATCTTTACTTC	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63961_Pcan_Bot2011_IGS	GERA15	1	BC63961_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA15	IGS	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	CGTAATCCTCAGTACTGGCTA	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63961_Pper_Dia2013_COI	GERA15	1	BC63961_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Pper_Dia2013_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	CTTAGTGGCATTAATTCGDAATCC	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63961_Sspa_Oph2007_COI	GERA15	1	BC63961_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	GAAGGCCTTCCGATGATC	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63961_Upin_Bot2011_COI	GERA15	1	BC63961_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Upin_Bot2011_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	CTTTAATTACAGCGTTTTTATTGTTGT	114.59997
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63962_Cjap_Sim2018_COI	GERA14	1	BC63962_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63962_Mgig_Bot2012_COI	GERA14	1	BC63962_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	TCTTATTCGTTGGAGACTTTATAACCCT	114.60049
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63963_Cmae_Bot2011_COI	GERA13	1	BC63963_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	ATGAACAGTCTATCCTCCTTTAG	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63963_Msal_Bot2012_COI	GERA13	1	BC63963_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Msal_Bot2012_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	GYTAGTTCCRATGATGTTAGCTG	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63963_Pvir_Dia2013_COI	GERA13	1	BC63963_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	CTTAGTGGCATTAATTCGDAATCC	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63963_Rhar_Sim2018_COI	GERA13	1	BC63963_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	CTGCTGCTATTGCTCATG	114.5987
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63965_Aamu_Bax2006_COI	GERA11	1	BC63965_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	GCACAACCGGGATCTTTACTTC	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63965_Pcan_Bot2011_IGS	GERA11	1	BC63965_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA11	IGS	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	CGTAATCCTCAGTACTGGCTA	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63965_Pper_Dia2013_COI	GERA11	1	BC63965_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Pper_Dia2013_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	CTTAGTGGCATTAATTCGDAATCC	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63965_Sspa_Oph2007_COI	GERA11	1	BC63965_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	GAAGGCCTTCCGATGATC	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63965_Upin_Bot2011_COI	GERA11	1	BC63965_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Upin_Bot2011_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	CTTTAATTACAGCGTTTTTATTGTTGT	114.59609
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63966_Cjap_Sim2018_COI	GERA10	1	BC63966_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63966_Mgig_Bot2012_COI	GERA10	1	BC63966_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	TCTTATTCGTTGGAGACTTTATAACCCT	114.60115
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63967_Cmae_Bot2011_COI	GERA9	1	BC63967_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	ATGAACAGTCTATCCTCCTTTAG	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63967_Msal_Bot2012_COI	GERA9	1	BC63967_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Msal_Bot2012_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	GYTAGTTCCRATGATGTTAGCTG	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63967_Pvir_Dia2013_COI	GERA9	1	BC63967_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	CTTAGTGGCATTAATTCGDAATCC	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63967_Rhar_Sim2018_COI	GERA9	1	BC63967_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	CTGCTGCTATTGCTCATG	114.5995
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63969_Aamu_Bax2006_COI	GERA7	1	BC63969_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	GCACAACCGGGATCTTTACTTC	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63969_Pcan_Bot2011_IGS	GERA7	1	BC63969_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA7	IGS	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	CGTAATCCTCAGTACTGGCTA	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63969_Pper_Dia2013_COI	GERA7	1	BC63969_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Pper_Dia2013_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	CTTAGTGGCATTAATTCGDAATCC	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63969_Sspa_Oph2007_COI	GERA7	1	BC63969_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	GAAGGCCTTCCGATGATC	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63969_Upin_Bot2011_COI	GERA7	1	BC63969_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Upin_Bot2011_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	CTTTAATTACAGCGTTTTTATTGTTGT	114.59669
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63970_Cmae_Bot2011_COI	GERA6	1	BC63970_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	ATGAACAGTCTATCCTCCTTTAG	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63970_Msal_Bot2012_COI	GERA6	1	BC63970_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Msal_Bot2012_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	GYTAGTTCCRATGATGTTAGCTG	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63970_Pvir_Dia2013_COI	GERA6	1	BC63970_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	CTTAGTGGCATTAATTCGDAATCC	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63970_Rhar_Sim2018_COI	GERA6	1	BC63970_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	CTGCTGCTATTGCTCATG	114.6002
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63972_Aamu_Bax2006_COI	GERA4	1	BC63972_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	GCACAACCGGGATCTTTACTTC	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63972_Pcan_Bot2011_IGS	GERA4	1	BC63972_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA4	IGS	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	CGTAATCCTCAGTACTGGCTA	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63972_Pper_Dia2013_COI	GERA4	1	BC63972_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Pper_Dia2013_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	CTTAGTGGCATTAATTCGDAATCC	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63972_Sspa_Oph2007_COI	GERA4	1	BC63972_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	GAAGGCCTTCCGATGATC	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63972_Upin_Bot2011_COI	GERA4	1	BC63972_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Upin_Bot2011_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	CTTTAATTACAGCGTTTTTATTGTTGT	114.59705
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63973_Cjap_Sim2018_COI	GERA3	1	BC63973_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63973_Mgig_Bot2012_COI	GERA3	1	BC63973_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	TCTTATTCGTTGGAGACTTTATAACCCT	114.59549
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63974_Cmae_Bot2011_COI	GERA2	1	BC63974_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	ATGAACAGTCTATCCTCCTTTAG	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63974_Msal_Bot2012_COI	GERA2	1	BC63974_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Msal_Bot2012_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	GYTAGTTCCRATGATGTTAGCTG	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63974_Pvir_Dia2013_COI	GERA2	1	BC63974_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	CTTAGTGGCATTAATTCGDAATCC	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63974_Rhar_Sim2018_COI	GERA2	1	BC63974_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	CTGCTGCTATTGCTCATG	114.59598
BC64564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81059	WGS84	BC64564	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64564_Cmae_Bot2011_COI	ADEAPC-60	1	BC64564_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64564	Sample	BC64564	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-60	COI	45	BC64564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-60	ATGAACAGTCTATCCTCCTTTAG	138.54369
BC64566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81333	WGS84	BC64566	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64566_Aamu_Bax2006_COI	ADEAPC-58	1	BC64566_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64566	Sample	BC64566	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-58	COI	45	BC64566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-58	GCACAACCGGGATCTTTACTTC	138.53131
BC64566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81333	WGS84	BC64566	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64566_Pcan_Bot2011_IGS	ADEAPC-58	1	BC64566_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64566	Sample	BC64566	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-58	IGS	45	BC64566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-58	CGTAATCCTCAGTACTGGCTA	138.53131
BC64566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81333	WGS84	BC64566	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.764	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64566_Sspa_Oph2007_COI	ADEAPC-58	1	BC64566_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64566	Sample	BC64566	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-58	COI	45	BC64566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-58	GAAGGCCTTCCGATGATC	138.53131
BC64566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81333	WGS84	BC64566	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64566_Upin_Bot2011_COI	ADEAPC-58	1	BC64566_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64566	Sample	BC64566	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-58	COI	45	BC64566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-58	CTTTAATTACAGCGTTTTTATTGTTGT	138.53131
BC64567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81246	WGS84	BC64567	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64567_Cjap_Sim2018_COI	ADEAPC-57	1	BC64567_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64567	Sample	BC64567	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-57	COI	45	BC64567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-57	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52837
BC64567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81246	WGS84	BC64567	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.236	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64567_Mgig_Bot2012_COI	ADEAPC-57	1	BC64567_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64567	Sample	BC64567	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-57	COI	45	BC64567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-57	TCTTATTCGTTGGAGACTTTATAACCCT	138.52837
BC64568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81501	WGS84	BC64568	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64568_Cmae_Bot2011_COI	ADEAPC-56	1	BC64568_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64568	Sample	BC64568	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-56	COI	45	BC64568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-56	ATGAACAGTCTATCCTCCTTTAG	138.51475
BC64570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83039	WGS84	BC64570	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64570_Cjap_Sim2018_COI	ADEAPC-54	1	BC64570_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64570	Sample	BC64570	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-54	COI	45	BC64570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-54	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51097
BC64570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83039	WGS84	BC64570	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.667	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64570_Mgig_Bot2012_COI	ADEAPC-54	1	BC64570_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64570	Sample	BC64570	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-54	COI	45	BC64570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-54	TCTTATTCGTTGGAGACTTTATAACCCT	138.51097
BC64571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83047	WGS84	BC64571	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64571_Cmae_Bot2011_COI	ADEAPC-53	1	BC64571_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64571	Sample	BC64571	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-53	COI	45	BC64571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-53	ATGAACAGTCTATCCTCCTTTAG	138.51076
BC64573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83073	WGS84	BC64573	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64573_Aamu_Bax2006_COI	ADEAPC-51	1	BC64573_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64573	Sample	BC64573	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-51	COI	45	BC64573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-51	GCACAACCGGGATCTTTACTTC	138.51131
BC64573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83073	WGS84	BC64573	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64573_Pcan_Bot2011_IGS	ADEAPC-51	1	BC64573_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64573	Sample	BC64573	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-51	IGS	45	BC64573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-51	CGTAATCCTCAGTACTGGCTA	138.51131
BC64573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83073	WGS84	BC64573	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.457	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64573_Sspa_Oph2007_COI	ADEAPC-51	1	BC64573_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64573	Sample	BC64573	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-51	COI	45	BC64573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-51	GAAGGCCTTCCGATGATC	138.51131
BC64573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83073	WGS84	BC64573	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64573_Upin_Bot2011_COI	ADEAPC-51	1	BC64573_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64573	Sample	BC64573	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-51	COI	45	BC64573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-51	CTTTAATTACAGCGTTTTTATTGTTGT	138.51131
BC64574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83719	WGS84	BC64574	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64574_Cjap_Sim2018_COI	ADEAPC-50	1	BC64574_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64574	Sample	BC64574	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-50	COI	45	BC64574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-50	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5105
BC64574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83719	WGS84	BC64574	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.492	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64574_Mgig_Bot2012_COI	ADEAPC-50	1	BC64574_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64574	Sample	BC64574	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-50	COI	45	BC64574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-50	TCTTATTCGTTGGAGACTTTATAACCCT	138.5105
BC64575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83738	WGS84	BC64575	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64575_Cmae_Bot2011_COI	ADEAPC-49	1	BC64575_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64575	Sample	BC64575	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-49	COI	45	BC64575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-49	ATGAACAGTCTATCCTCCTTTAG	138.51065
BC64577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83723	WGS84	BC64577	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64577_Aamu_Bax2006_COI	ADEAPC-47	1	BC64577_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64577	Sample	BC64577	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-47	COI	45	BC64577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-47	GCACAACCGGGATCTTTACTTC	138.5104
BC64577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83723	WGS84	BC64577	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64577_Pcan_Bot2011_IGS	ADEAPC-47	1	BC64577_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64577	Sample	BC64577	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-47	IGS	45	BC64577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-47	CGTAATCCTCAGTACTGGCTA	138.5104
BC64577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83723	WGS84	BC64577	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.153	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64577_Sspa_Oph2007_COI	ADEAPC-47	1	BC64577_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64577	Sample	BC64577	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-47	COI	45	BC64577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-47	GAAGGCCTTCCGATGATC	138.5104
BC64577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83723	WGS84	BC64577	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64577_Upin_Bot2011_COI	ADEAPC-47	1	BC64577_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64577	Sample	BC64577	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-47	COI	45	BC64577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-47	CTTTAATTACAGCGTTTTTATTGTTGT	138.5104
BC64578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8079	WGS84	BC64578	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64578_Cjap_Sim2018_COI	ADEAPC-46	1	BC64578_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64578	Sample	BC64578	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-46	COI	45	BC64578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-46	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54826
BC64578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8079	WGS84	BC64578	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64578_Mgig_Bot2012_COI	ADEAPC-46	1	BC64578_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64578	Sample	BC64578	targeted	Absent	Sample	Mgig_Bot2012_COI	ADEAPC-46	COI	45	BC64578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-46	TCTTATTCGTTGGAGACTTTATAACCCT	138.54826
BC64579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80372	WGS84	BC64579	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64579_Cmae_Bot2011_COI	ADEAPC-45	1	BC64579_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64579	Sample	BC64579	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-45	COI	45	BC64579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-45	ATGAACAGTCTATCCTCCTTTAG	138.54048
BC64580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80499	WGS84	BC64580	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64580_Aamu_Bax2006_COI	ADEAPC-44	1	BC64580_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64580	Sample	BC64580	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-44	COI	45	BC64580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-44	GCACAACCGGGATCTTTACTTC	138.54584
BC64580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80499	WGS84	BC64580	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64580_Pcan_Bot2011_IGS	ADEAPC-44	1	BC64580_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64580	Sample	BC64580	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-44	IGS	45	BC64580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-44	CGTAATCCTCAGTACTGGCTA	138.54584
BC64580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80499	WGS84	BC64580	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.754	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64580_Sspa_Oph2007_COI	ADEAPC-44	1	BC64580_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64580	Sample	BC64580	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-44	COI	45	BC64580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-44	GAAGGCCTTCCGATGATC	138.54584
BC64580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80499	WGS84	BC64580	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64580_Upin_Bot2011_COI	ADEAPC-44	1	BC64580_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64580	Sample	BC64580	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-44	COI	45	BC64580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-44	CTTTAATTACAGCGTTTTTATTGTTGT	138.54584
BC64581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80329	WGS84	BC64581	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64581_Cjap_Sim2018_COI	ADEAPC-43	1	BC64581_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64581	Sample	BC64581	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-43	COI	45	BC64581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-43	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54832
BC64581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80329	WGS84	BC64581	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.483	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64581_Mgig_Bot2012_COI	ADEAPC-43	1	BC64581_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64581	Sample	BC64581	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-43	COI	45	BC64581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-43	TCTTATTCGTTGGAGACTTTATAACCCT	138.54832
BC64582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8005	WGS84	BC64582	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64582_Cmae_Bot2011_COI	ADEAPC-42	1	BC64582_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64582	Sample	BC64582	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-42	COI	45	BC64582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-42	ATGAACAGTCTATCCTCCTTTAG	138.54783
BC64584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79447	WGS84	BC64584	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64584_Aamu_Bax2006_COI	ADEAPC-40	1	BC64584_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64584	Sample	BC64584	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-40	COI	45	BC64584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-40	GCACAACCGGGATCTTTACTTC	138.54594
BC64584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79447	WGS84	BC64584	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64584_Pcan_Bot2011_IGS	ADEAPC-40	1	BC64584_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64584	Sample	BC64584	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-40	IGS	45	BC64584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-40	CGTAATCCTCAGTACTGGCTA	138.54594
BC64584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79447	WGS84	BC64584	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64584_Sspa_Oph2007_COI	ADEAPC-40	1	BC64584_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64584	Sample	BC64584	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-40	COI	45	BC64584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-40	GAAGGCCTTCCGATGATC	138.54594
BC64584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79447	WGS84	BC64584	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64584_Upin_Bot2011_COI	ADEAPC-40	1	BC64584_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64584	Sample	BC64584	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-40	COI	45	BC64584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-40	CTTTAATTACAGCGTTTTTATTGTTGT	138.54594
BC64585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79078	WGS84	BC64585	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64585_Cjap_Sim2018_COI	ADEAPC-39	1	BC64585_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64585	Sample	BC64585	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-39	COI	45	BC64585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-39	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54425
BC64585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79078	WGS84	BC64585	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.475	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64585_Mgig_Bot2012_COI	ADEAPC-39	1	BC64585_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64585	Sample	BC64585	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-39	COI	45	BC64585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-39	TCTTATTCGTTGGAGACTTTATAACCCT	138.54425
BC64586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78712	WGS84	BC64586	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64586_Cmae_Bot2011_COI	ADEAPC-38	1	BC64586_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64586	Sample	BC64586	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-38	COI	45	BC64586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-38	ATGAACAGTCTATCCTCCTTTAG	138.54309
BC64588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78076	WGS84	BC64588	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64588_Aamu_Bax2006_COI	ADEAPC-36	1	BC64588_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64588	Sample	BC64588	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-36	COI	45	BC64588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-36	GCACAACCGGGATCTTTACTTC	138.5374
BC64588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78076	WGS84	BC64588	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64588_Pcan_Bot2011_IGS	ADEAPC-36	1	BC64588_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64588	Sample	BC64588	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-36	IGS	45	BC64588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-36	CGTAATCCTCAGTACTGGCTA	138.5374
BC64588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78076	WGS84	BC64588	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.269	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64588_Sspa_Oph2007_COI	ADEAPC-36	1	BC64588_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64588	Sample	BC64588	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-36	COI	45	BC64588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-36	GAAGGCCTTCCGATGATC	138.5374
BC64588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78076	WGS84	BC64588	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64588_Upin_Bot2011_COI	ADEAPC-36	1	BC64588_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64588	Sample	BC64588	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-36	COI	45	BC64588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-36	CTTTAATTACAGCGTTTTTATTGTTGT	138.5374
BC64589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77762	WGS84	BC64589	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64589_Cjap_Sim2018_COI	ADEAPC-35	1	BC64589_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64589	Sample	BC64589	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-35	COI	45	BC64589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-35	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.53481
BC64589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77762	WGS84	BC64589	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.325	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64589_Mgig_Bot2012_COI	ADEAPC-35	1	BC64589_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64589	Sample	BC64589	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-35	COI	45	BC64589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-35	TCTTATTCGTTGGAGACTTTATAACCCT	138.53481
BC64591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77128	WGS84	BC64591	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64591_Aamu_Bax2006_COI	ADEAPC-33	1	BC64591_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64591	Sample	BC64591	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-33	COI	45	BC64591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-33	GCACAACCGGGATCTTTACTTC	138.52947
BC64591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77128	WGS84	BC64591	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64591_Pcan_Bot2011_IGS	ADEAPC-33	1	BC64591_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64591	Sample	BC64591	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-33	IGS	45	BC64591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-33	CGTAATCCTCAGTACTGGCTA	138.52947
BC64591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77128	WGS84	BC64591	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64591_Sspa_Oph2007_COI	ADEAPC-33	1	BC64591_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64591	Sample	BC64591	targeted	Absent	Sample	Sspa_Oph2007_COI	ADEAPC-33	COI	45	BC64591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-33	GAAGGCCTTCCGATGATC	138.52947
BC64591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77128	WGS84	BC64591	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64591_Upin_Bot2011_COI	ADEAPC-33	1	BC64591_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64591	Sample	BC64591	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-33	COI	45	BC64591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-33	CTTTAATTACAGCGTTTTTATTGTTGT	138.52947
BC64592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76757	WGS84	BC64592	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64592_Cjap_Sim2018_COI	ADEAPC-32	1	BC64592_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64592	Sample	BC64592	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-32	COI	45	BC64592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-32	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5283
BC64592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76757	WGS84	BC64592	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64592_Mgig_Bot2012_COI	ADEAPC-32	1	BC64592_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64592	Sample	BC64592	targeted	Absent	Sample	Mgig_Bot2012_COI	ADEAPC-32	COI	45	BC64592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-32	TCTTATTCGTTGGAGACTTTATAACCCT	138.5283
BC64593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76424	WGS84	BC64593	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64593_Cmae_Bot2011_COI	ADEAPC-31	1	BC64593_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64593	Sample	BC64593	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-31	COI	45	BC64593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-31	ATGAACAGTCTATCCTCCTTTAG	138.52591
BC64595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.75796	WGS84	BC64595	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64595_Aamu_Bax2006_COI	ADEAPC-29	1	BC64595_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64595	Sample	BC64595	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-29	COI	45	BC64595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-29	GCACAACCGGGATCTTTACTTC	138.52051
BC64595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.75796	WGS84	BC64595	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64595_Pcan_Bot2011_IGS	ADEAPC-29	1	BC64595_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64595	Sample	BC64595	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-29	IGS	45	BC64595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-29	CGTAATCCTCAGTACTGGCTA	138.52051
BC64595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.75796	WGS84	BC64595	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.047	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64595_Sspa_Oph2007_COI	ADEAPC-29	1	BC64595_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64595	Sample	BC64595	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-29	COI	45	BC64595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-29	GAAGGCCTTCCGATGATC	138.52051
BC64595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.75796	WGS84	BC64595	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64595_Upin_Bot2011_COI	ADEAPC-29	1	BC64595_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64595	Sample	BC64595	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-29	COI	45	BC64595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-29	CTTTAATTACAGCGTTTTTATTGTTGT	138.52051
BC64596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81098	WGS84	BC64596	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64596_Cjap_Sim2018_COI	ADEAPC-28	1	BC64596_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64596	Sample	BC64596	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-28	COI	45	BC64596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-28	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52321
BC64596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81098	WGS84	BC64596	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.994	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64596_Mgig_Bot2012_COI	ADEAPC-28	1	BC64596_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64596	Sample	BC64596	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-28	COI	45	BC64596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-28	TCTTATTCGTTGGAGACTTTATAACCCT	138.52321
BC64597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81029	WGS84	BC64597	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64597_Cmae_Bot2011_COI	ADEAPC-27	1	BC64597_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64597	Sample	BC64597	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-27	COI	45	BC64597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-27	ATGAACAGTCTATCCTCCTTTAG	138.52168
BC64599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80869	WGS84	BC64599	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64599_Aamu_Bax2006_COI	ADEAPC-25	1	BC64599_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64599	Sample	BC64599	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-25	COI	45	BC64599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-25	GCACAACCGGGATCTTTACTTC	138.5177
BC64599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80869	WGS84	BC64599	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64599_Pcan_Bot2011_IGS	ADEAPC-25	1	BC64599_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64599	Sample	BC64599	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-25	IGS	45	BC64599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-25	CGTAATCCTCAGTACTGGCTA	138.5177
BC64599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80869	WGS84	BC64599	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.117	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64599_Sspa_Oph2007_COI	ADEAPC-25	1	BC64599_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64599	Sample	BC64599	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-25	COI	45	BC64599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-25	GAAGGCCTTCCGATGATC	138.5177
BC64599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80869	WGS84	BC64599	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64599_Upin_Bot2011_COI	ADEAPC-25	1	BC64599_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64599	Sample	BC64599	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-25	COI	45	BC64599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-25	CTTTAATTACAGCGTTTTTATTGTTGT	138.5177
BC64600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80373	WGS84	BC64600	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64600_Cjap_Sim2018_COI	ADEAPC-24	1	BC64600_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64600	Sample	BC64600	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-24	COI	45	BC64600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-24	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51423
BC64600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80373	WGS84	BC64600	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.15	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64600_Mgig_Bot2012_COI	ADEAPC-24	1	BC64600_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64600	Sample	BC64600	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-24	COI	45	BC64600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-24	TCTTATTCGTTGGAGACTTTATAACCCT	138.51423
BC64601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80038	WGS84	BC64601	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64601_Cmae_Bot2011_COI	ADEAPC-23	1	BC64601_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64601	Sample	BC64601	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-23	COI	45	BC64601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-23	ATGAACAGTCTATCCTCCTTTAG	138.51358
BC64603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79528	WGS84	BC64603	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64603_Aamu_Bax2006_COI	ADEAPC-21	1	BC64603_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64603	Sample	BC64603	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-21	COI	45	BC64603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-21	GCACAACCGGGATCTTTACTTC	138.51364
BC64603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79528	WGS84	BC64603	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64603_Pcan_Bot2011_IGS	ADEAPC-21	1	BC64603_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64603	Sample	BC64603	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-21	IGS	45	BC64603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-21	CGTAATCCTCAGTACTGGCTA	138.51364
BC64603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79528	WGS84	BC64603	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.123	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64603_Sspa_Oph2007_COI	ADEAPC-21	1	BC64603_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64603	Sample	BC64603	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-21	COI	45	BC64603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-21	GAAGGCCTTCCGATGATC	138.51364
BC64603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79528	WGS84	BC64603	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64603_Upin_Bot2011_COI	ADEAPC-21	1	BC64603_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64603	Sample	BC64603	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-21	COI	45	BC64603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-21	CTTTAATTACAGCGTTTTTATTGTTGT	138.51364
BC64604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79054	WGS84	BC64604	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64604_Cjap_Sim2018_COI	ADEAPC-20	1	BC64604_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64604	Sample	BC64604	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-20	COI	45	BC64604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-20	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51486
BC64604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79054	WGS84	BC64604	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.167	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64604_Mgig_Bot2012_COI	ADEAPC-20	1	BC64604_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64604	Sample	BC64604	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-20	COI	45	BC64604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-20	TCTTATTCGTTGGAGACTTTATAACCCT	138.51486
BC64605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78832	WGS84	BC64605	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64605_Cmae_Bot2011_COI	ADEAPC-19	1	BC64605_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64605	Sample	BC64605	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-19	COI	45	BC64605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-19	ATGAACAGTCTATCCTCCTTTAG	138.51547
BC64607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78364	WGS84	BC64607	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64607_Aamu_Bax2006_COI	ADEAPC-17	1	BC64607_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64607	Sample	BC64607	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-17	COI	45	BC64607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-17	GCACAACCGGGATCTTTACTTC	138.51668
BC64607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78364	WGS84	BC64607	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64607_Pcan_Bot2011_IGS	ADEAPC-17	1	BC64607_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64607	Sample	BC64607	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-17	IGS	45	BC64607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-17	CGTAATCCTCAGTACTGGCTA	138.51668
BC64607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78364	WGS84	BC64607	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.492	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64607_Sspa_Oph2007_COI	ADEAPC-17	1	BC64607_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64607	Sample	BC64607	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-17	COI	45	BC64607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-17	GAAGGCCTTCCGATGATC	138.51668
BC64607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78364	WGS84	BC64607	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64607_Upin_Bot2011_COI	ADEAPC-17	1	BC64607_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64607	Sample	BC64607	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-17	COI	45	BC64607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.51668
BC64608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78074	WGS84	BC64608	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64608_Cjap_Sim2018_COI	ADEAPC-16	1	BC64608_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64608	Sample	BC64608	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-16	COI	45	BC64608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51766
BC64608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78074	WGS84	BC64608	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.918	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64608_Mgig_Bot2012_COI	ADEAPC-16	1	BC64608_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64608	Sample	BC64608	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-16	COI	45	BC64608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.51766
BC64609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.778	WGS84	BC64609	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64609_Cmae_Bot2011_COI	ADEAPC-15	1	BC64609_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64609	Sample	BC64609	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-15	COI	45	BC64609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-15	ATGAACAGTCTATCCTCCTTTAG	138.51913
BC64610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77268	WGS84	BC64610	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64610_Aamu_Bax2006_COI	ADEAPC-14	1	BC64610_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64610	Sample	BC64610	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-14	COI	45	BC64610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-14	GCACAACCGGGATCTTTACTTC	138.51969
BC64610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77268	WGS84	BC64610	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64610_Pcan_Bot2011_IGS	ADEAPC-14	1	BC64610_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64610	Sample	BC64610	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-14	IGS	45	BC64610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-14	CGTAATCCTCAGTACTGGCTA	138.51969
BC64610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77268	WGS84	BC64610	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.672	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64610_Sspa_Oph2007_COI	ADEAPC-14	1	BC64610_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64610	Sample	BC64610	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-14	COI	45	BC64610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-14	GAAGGCCTTCCGATGATC	138.51969
BC64610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77268	WGS84	BC64610	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64610_Upin_Bot2011_COI	ADEAPC-14	1	BC64610_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64610	Sample	BC64610	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-14	COI	45	BC64610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.51969
BC64611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76972	WGS84	BC64611	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64611_Cjap_Sim2018_COI	ADEAPC-13	1	BC64611_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64611	Sample	BC64611	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-13	COI	45	BC64611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51927
BC64611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76972	WGS84	BC64611	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.46	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64611_Mgig_Bot2012_COI	ADEAPC-13	1	BC64611_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64611	Sample	BC64611	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-13	COI	45	BC64611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.51927
BC64612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76536	WGS84	BC64612	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64612_Cmae_Bot2011_COI	ADEAPC-12	1	BC64612_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64612	Sample	BC64612	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-12	COI	45	BC64612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-12	ATGAACAGTCTATCCTCCTTTAG	138.51727
BC64614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76032	WGS84	BC64614	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64614_Aamu_Bax2006_COI	ADEAPC-10	1	BC64614_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64614	Sample	BC64614	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-10	COI	45	BC64614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-10	GCACAACCGGGATCTTTACTTC	138.50655
BC64614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76032	WGS84	BC64614	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64614_Pcan_Bot2011_IGS	ADEAPC-10	1	BC64614_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64614	Sample	BC64614	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-10	IGS	45	BC64614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-10	CGTAATCCTCAGTACTGGCTA	138.50655
BC64614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76032	WGS84	BC64614	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.543	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64614_Sspa_Oph2007_COI	ADEAPC-10	1	BC64614_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64614	Sample	BC64614	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-10	COI	45	BC64614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-10	GAAGGCCTTCCGATGATC	138.50655
BC64614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76032	WGS84	BC64614	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64614_Upin_Bot2011_COI	ADEAPC-10	1	BC64614_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64614	Sample	BC64614	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-10	COI	45	BC64614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.50655
BC64615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76007	WGS84	BC64615	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64615_Cjap_Sim2018_COI	ADEAPC-09	1	BC64615_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64615	Sample	BC64615	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-09	COI	45	BC64615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50254
BC64615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76007	WGS84	BC64615	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.176	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64615_Mgig_Bot2012_COI	ADEAPC-09	1	BC64615_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64615	Sample	BC64615	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-09	COI	45	BC64615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.50254
BC64616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76234	WGS84	BC64616	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64616_Cmae_Bot2011_COI	ADEAPC-08	1	BC64616_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64616	Sample	BC64616	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-08	COI	45	BC64616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-08	ATGAACAGTCTATCCTCCTTTAG	138.49388
BC64618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76372	WGS84	BC64618	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64618_Aamu_Bax2006_COI	ADEAPC-06	1	BC64618_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64618	Sample	BC64618	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-06	COI	45	BC64618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-06	GCACAACCGGGATCTTTACTTC	138.49151
BC64618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76372	WGS84	BC64618	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64618_Pcan_Bot2011_IGS	ADEAPC-06	1	BC64618_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64618	Sample	BC64618	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-06	IGS	45	BC64618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-06	CGTAATCCTCAGTACTGGCTA	138.49151
BC64618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76372	WGS84	BC64618	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.224	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64618_Sspa_Oph2007_COI	ADEAPC-06	1	BC64618_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64618	Sample	BC64618	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-06	COI	45	BC64618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-06	GAAGGCCTTCCGATGATC	138.49151
BC64618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76372	WGS84	BC64618	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64618_Upin_Bot2011_COI	ADEAPC-06	1	BC64618_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64618	Sample	BC64618	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-06	COI	45	BC64618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.49151
BC64619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76454	WGS84	BC64619	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64619_Cjap_Sim2018_COI	ADEAPC-05	1	BC64619_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64619	Sample	BC64619	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-05	COI	45	BC64619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49035
BC64619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76454	WGS84	BC64619	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.998	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64619_Mgig_Bot2012_COI	ADEAPC-05	1	BC64619_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64619	Sample	BC64619	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-05	COI	45	BC64619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.49035
BC64621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76978	WGS84	BC64621	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64621_Aamu_Bax2006_COI	ADEAPC-03	1	BC64621_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64621	Sample	BC64621	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-03	COI	45	BC64621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-03	GCACAACCGGGATCTTTACTTC	138.48298
BC64621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76978	WGS84	BC64621	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64621_Pcan_Bot2011_IGS	ADEAPC-03	1	BC64621_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64621	Sample	BC64621	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-03	IGS	45	BC64621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-03	CGTAATCCTCAGTACTGGCTA	138.48298
BC64621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76978	WGS84	BC64621	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.185	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64621_Sspa_Oph2007_COI	ADEAPC-03	1	BC64621_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64621	Sample	BC64621	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-03	COI	45	BC64621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-03	GAAGGCCTTCCGATGATC	138.48298
BC64621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76978	WGS84	BC64621	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64621_Upin_Bot2011_COI	ADEAPC-03	1	BC64621_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64621	Sample	BC64621	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-03	COI	45	BC64621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.48298
BC64622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.7704	WGS84	BC64622	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64622_Cjap_Sim2018_COI	ADEAPC-02	1	BC64622_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64622	Sample	BC64622	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-02	COI	45	BC64622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48253
BC64622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.7704	WGS84	BC64622	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64622_Mgig_Bot2012_COI	ADEAPC-02	1	BC64622_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64622	Sample	BC64622	targeted	Absent	Sample	Mgig_Bot2012_COI	ADEAPC-02	COI	45	BC64622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.48253
BC64623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77103	WGS84	BC64623	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64623_Cmae_Bot2011_COI	ADEAPC-01	1	BC64623_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64623	Sample	BC64623	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-01	COI	45	BC64623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-01	ATGAACAGTCTATCCTCCTTTAG	138.48203
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC76569_Cmae_Bot2011_COI	GERB1	1	BC76569_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	ATGAACAGTCTATCCTCCTTTAG	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC76569_Msal_Bot2012_COI	GERB1	1	BC76569_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Msal_Bot2012_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	GYTAGTTCCRATGATGTTAGCTG	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC76569_Pvir_Dia2013_COI	GERB1	1	BC76569_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	CTTAGTGGCATTAATTCGDAATCC	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC76569_Rhar_Sim2018_COI	GERB1	1	BC76569_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	CTGCTGCTATTGCTCATG	114.59414
BC77252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83697	WGS84	BC77252	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77252_Aamu_Bax2006_COI	S5-09	1	BC77252_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77252	Sample	BC77252	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-09	COI	45	BC77252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-09	GCACAACCGGGATCTTTACTTC	138.51049
BC77252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83697	WGS84	BC77252	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77252_Pcan_Bot2011_IGS	S5-09	1	BC77252_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77252	Sample	BC77252	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-09	IGS	45	BC77252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-09	CGTAATCCTCAGTACTGGCTA	138.51049
BC77252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83697	WGS84	BC77252	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.282	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77252_Sspa_Oph2007_COI	S5-09	1	BC77252_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77252	Sample	BC77252	targeted	Present	Sample	Sspa_Oph2007_COI	S5-09	COI	45	BC77252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-09	GAAGGCCTTCCGATGATC	138.51049
BC77252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83697	WGS84	BC77252	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77252_Upin_Bot2011_COI	S5-09	1	BC77252_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77252	Sample	BC77252	targeted	Absent	Sample	Upin_Bot2011_COI	S5-09	COI	45	BC77252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.51049
BC77253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78389	WGS84	BC77253	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77253_Cjap_Sim2018_COI	S5-14	1	BC77253_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77253	Sample	BC77253	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-14	COI	45	BC77253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51561
BC77253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78389	WGS84	BC77253	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.215	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77253_Mgig_Bot2012_COI	S5-14	1	BC77253_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77253	Sample	BC77253	targeted	Present	Sample	Mgig_Bot2012_COI	S5-14	COI	45	BC77253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.51561
BC77254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77376	WGS84	BC77254	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77254_Cmae_Bot2011_COI	S5-19	1	BC77254_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77254	Sample	BC77254	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-19	COI	45	BC77254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-19	ATGAACAGTCTATCCTCCTTTAG	138.48542
BC77256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84392	WGS84	BC77256	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77256_Aamu_Bax2006_COI	S6-04	1	BC77256_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77256	Sample	BC77256	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-04	COI	45	BC77256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-04	GCACAACCGGGATCTTTACTTC	138.49698
BC77256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84392	WGS84	BC77256	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77256_Pcan_Bot2011_IGS	S6-04	1	BC77256_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77256	Sample	BC77256	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-04	IGS	45	BC77256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-04	CGTAATCCTCAGTACTGGCTA	138.49698
BC77256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84392	WGS84	BC77256	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77256_Sspa_Oph2007_COI	S6-04	1	BC77256_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77256	Sample	BC77256	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-04	COI	45	BC77256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-04	GAAGGCCTTCCGATGATC	138.49698
BC77256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84392	WGS84	BC77256	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77256_Upin_Bot2011_COI	S6-04	1	BC77256_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77256	Sample	BC77256	targeted	Absent	Sample	Upin_Bot2011_COI	S6-04	COI	45	BC77256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.49698
BC77257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83669	WGS84	BC77257	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77257_Cjap_Sim2018_COI	S6-09	1	BC77257_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77257	Sample	BC77257	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-09	COI	45	BC77257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5098
BC77257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83669	WGS84	BC77257	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77257_Mgig_Bot2012_COI	S6-09	1	BC77257_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77257	Sample	BC77257	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-09	COI	45	BC77257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.5098
BC77258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78399	WGS84	BC77258	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77258_Cmae_Bot2011_COI	S6-14	1	BC77258_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77258	Sample	BC77258	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-14	COI	45	BC77258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-14	ATGAACAGTCTATCCTCCTTTAG	138.51578
BC77260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81082	WGS84	BC77260	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77260_Cjap_Sim2018_COI	S6-23	1	BC77260_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77260	Sample	BC77260	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-23	COI	45	BC77260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-23	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52258
BC77260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81082	WGS84	BC77260	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77260_Mgig_Bot2012_COI	S6-23	1	BC77260_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77260	Sample	BC77260	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-23	COI	45	BC77260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-23	TCTTATTCGTTGGAGACTTTATAACCCT	138.52258
BC77261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84489	WGS84	BC77261	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.372	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77261_Cmae_Bot2011_COI	S7-04	1	BC77261_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77261	Sample	BC77261	targeted	Present	Sample	Cmae_Bot2011_COI	S7-04	COI	45	BC77261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-04	ATGAACAGTCTATCCTCCTTTAG	138.49712
BC77263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78246	WGS84	BC77263	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77263_Aamu_Bax2006_COI	S7-14	1	BC77263_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77263	Sample	BC77263	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-14	COI	45	BC77263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-14	GCACAACCGGGATCTTTACTTC	138.51648
BC77263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78246	WGS84	BC77263	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77263_Pcan_Bot2011_IGS	S7-14	1	BC77263_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77263	Sample	BC77263	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-14	IGS	45	BC77263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-14	CGTAATCCTCAGTACTGGCTA	138.51648
BC77263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78246	WGS84	BC77263	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.023	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77263_Sspa_Oph2007_COI	S7-14	1	BC77263_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77263	Sample	BC77263	targeted	Present	Sample	Sspa_Oph2007_COI	S7-14	COI	45	BC77263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-14	GAAGGCCTTCCGATGATC	138.51648
BC77263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78246	WGS84	BC77263	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77263_Upin_Bot2011_COI	S7-14	1	BC77263_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77263	Sample	BC77263	targeted	Absent	Sample	Upin_Bot2011_COI	S7-14	COI	45	BC77263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.51648
BC77264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77342	WGS84	BC77264	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77264_Cjap_Sim2018_COI	S7-19	1	BC77264_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77264	Sample	BC77264	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-19	COI	45	BC77264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48496
BC77264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77342	WGS84	BC77264	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.021	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77264_Mgig_Bot2012_COI	S7-19	1	BC77264_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77264	Sample	BC77264	targeted	Present	Sample	Mgig_Bot2012_COI	S7-19	COI	45	BC77264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.48496
BC77265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81053	WGS84	BC77265	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.653	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77265_Cmae_Bot2011_COI	S7-23	1	BC77265_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77265	Sample	BC77265	targeted	Present	Sample	Cmae_Bot2011_COI	S7-23	COI	45	BC77265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-23	ATGAACAGTCTATCCTCCTTTAG	138.52131
BC77267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78552	WGS84	BC77267	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77267_Aamu_Bax2006_COI	S8-09	1	BC77267_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77267	Sample	BC77267	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-09	COI	45	BC77267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-09	GCACAACCGGGATCTTTACTTC	138.51387
BC77267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78552	WGS84	BC77267	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77267_Pcan_Bot2011_IGS	S8-09	1	BC77267_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77267	Sample	BC77267	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-09	IGS	45	BC77267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-09	CGTAATCCTCAGTACTGGCTA	138.51387
BC77267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78552	WGS84	BC77267	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.316	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77267_Sspa_Oph2007_COI	S8-09	1	BC77267_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77267	Sample	BC77267	targeted	Present	Sample	Sspa_Oph2007_COI	S8-09	COI	45	BC77267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-09	GAAGGCCTTCCGATGATC	138.51387
BC77267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78552	WGS84	BC77267	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77267_Upin_Bot2011_COI	S8-09	1	BC77267_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77267	Sample	BC77267	targeted	Absent	Sample	Upin_Bot2011_COI	S8-09	COI	45	BC77267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.51387
BC77268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7737	WGS84	BC77268	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77268_Cjap_Sim2018_COI	S8-14	1	BC77268_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77268	Sample	BC77268	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-14	COI	45	BC77268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48409
BC77268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7737	WGS84	BC77268	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77268_Mgig_Bot2012_COI	S8-14	1	BC77268_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77268	Sample	BC77268	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-14	COI	45	BC77268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.48409
BC77269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84801	WGS84	BC77269	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77269_Cmae_Bot2011_COI	S8-19	1	BC77269_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77269	Sample	BC77269	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-19	COI	45	BC77269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-19	ATGAACAGTCTATCCTCCTTTAG	138.49777
BC77270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83692	WGS84	BC77270	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77270_Aamu_Bax2006_COI	S8-23	1	BC77270_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77270	Sample	BC77270	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-23	COI	45	BC77270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-23	GCACAACCGGGATCTTTACTTC	138.50975
BC77270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83692	WGS84	BC77270	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77270_Pcan_Bot2011_IGS	S8-23	1	BC77270_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77270	Sample	BC77270	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-23	IGS	45	BC77270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-23	CGTAATCCTCAGTACTGGCTA	138.50975
BC77270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83692	WGS84	BC77270	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.554	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77270_Sspa_Oph2007_COI	S8-23	1	BC77270_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77270	Sample	BC77270	targeted	Present	Sample	Sspa_Oph2007_COI	S8-23	COI	45	BC77270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-23	GAAGGCCTTCCGATGATC	138.50975
BC77270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83692	WGS84	BC77270	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77270_Upin_Bot2011_COI	S8-23	1	BC77270_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77270	Sample	BC77270	targeted	Absent	Sample	Upin_Bot2011_COI	S8-23	COI	45	BC77270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-23	CTTTAATTACAGCGTTTTTATTGTTGT	138.50975
BC77271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77271	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77271_Cjap_Sim2018_COI	S16-10	1	BC77271_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77271	Sample	BC77271	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-10	COI	45	BC77271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52039
BC77271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77271	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.1	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77271_Mgig_Bot2012_COI	S16-10	1	BC77271_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77271	Sample	BC77271	targeted	Present	Sample	Mgig_Bot2012_COI	S16-10	COI	45	BC77271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.52039
BC77272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8366	WGS84	BC77272	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77272_Cmae_Bot2011_COI	S16-02	1	BC77272_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77272	Sample	BC77272	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-02	COI	45	BC77272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-02	ATGAACAGTCTATCCTCCTTTAG	138.50718
BC77274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83522	WGS84	BC77274	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77274_Aamu_Bax2006_COI	S16-04	1	BC77274_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77274	Sample	BC77274	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-04	COI	45	BC77274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-04	GCACAACCGGGATCTTTACTTC	138.50734
BC77274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83522	WGS84	BC77274	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77274_Pcan_Bot2011_IGS	S16-04	1	BC77274_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77274	Sample	BC77274	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-04	IGS	45	BC77274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-04	CGTAATCCTCAGTACTGGCTA	138.50734
BC77274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83522	WGS84	BC77274	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77274_Sspa_Oph2007_COI	S16-04	1	BC77274_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77274	Sample	BC77274	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-04	COI	45	BC77274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-04	GAAGGCCTTCCGATGATC	138.50734
BC77274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83522	WGS84	BC77274	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77274_Upin_Bot2011_COI	S16-04	1	BC77274_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77274	Sample	BC77274	targeted	Absent	Sample	Upin_Bot2011_COI	S16-04	COI	45	BC77274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.50734
BC77275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83426	WGS84	BC77275	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77275_Cjap_Sim2018_COI	S16-05	1	BC77275_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77275	Sample	BC77275	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-05	COI	45	BC77275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50758
BC77275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83426	WGS84	BC77275	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.713	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77275_Mgig_Bot2012_COI	S16-05	1	BC77275_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77275	Sample	BC77275	targeted	Present	Sample	Mgig_Bot2012_COI	S16-05	COI	45	BC77275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.50758
BC77276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80831	WGS84	BC77276	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77276_Cmae_Bot2011_COI	S16-06	1	BC77276_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77276	Sample	BC77276	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-06	COI	45	BC77276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-06	ATGAACAGTCTATCCTCCTTTAG	138.516
BC77278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77278	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77278_Aamu_Bax2006_COI	S16-08	1	BC77278_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77278	Sample	BC77278	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-08	COI	45	BC77278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-08	GCACAACCGGGATCTTTACTTC	138.51838
BC77278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77278	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77278_Pcan_Bot2011_IGS	S16-08	1	BC77278_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77278	Sample	BC77278	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-08	IGS	45	BC77278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-08	CGTAATCCTCAGTACTGGCTA	138.51838
BC77278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77278	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77278_Sspa_Oph2007_COI	S16-08	1	BC77278_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77278	Sample	BC77278	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-08	COI	45	BC77278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-08	GAAGGCCTTCCGATGATC	138.51838
BC77278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77278	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77278_Upin_Bot2011_COI	S16-08	1	BC77278_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77278	Sample	BC77278	targeted	Absent	Sample	Upin_Bot2011_COI	S16-08	COI	45	BC77278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51838
BC77279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80992	WGS84	BC77279	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77279_Cjap_Sim2018_COI	S16-09	1	BC77279_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77279	Sample	BC77279	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-09	COI	45	BC77279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51928
BC77279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80992	WGS84	BC77279	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77279_Mgig_Bot2012_COI	S16-09	1	BC77279_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77279	Sample	BC77279	targeted	Absent	Sample	Mgig_Bot2012_COI	S16-09	COI	45	BC77279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51928
BC77281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77315	WGS84	BC77281	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77281_Aamu_Bax2006_COI	S17-01	1	BC77281_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77281	Sample	BC77281	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-01	COI	45	BC77281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-01	GCACAACCGGGATCTTTACTTC	138.48546
BC77281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77315	WGS84	BC77281	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77281_Pcan_Bot2011_IGS	S17-01	1	BC77281_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77281	Sample	BC77281	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-01	IGS	45	BC77281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-01	CGTAATCCTCAGTACTGGCTA	138.48546
BC77281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77315	WGS84	BC77281	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77281_Sspa_Oph2007_COI	S17-01	1	BC77281_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77281	Sample	BC77281	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-01	COI	45	BC77281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-01	GAAGGCCTTCCGATGATC	138.48546
BC77281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77315	WGS84	BC77281	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77281_Upin_Bot2011_COI	S17-01	1	BC77281_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77281	Sample	BC77281	targeted	Absent	Sample	Upin_Bot2011_COI	S17-01	COI	45	BC77281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.48546
BC77282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77369	WGS84	BC77282	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77282_Cjap_Sim2018_COI	S17-02	1	BC77282_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77282	Sample	BC77282	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-02	COI	45	BC77282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48594
BC77282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77369	WGS84	BC77282	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.037	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77282_Mgig_Bot2012_COI	S17-02	1	BC77282_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77282	Sample	BC77282	targeted	Present	Sample	Mgig_Bot2012_COI	S17-02	COI	45	BC77282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.48594
BC77283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77394	WGS84	BC77283	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77283_Cmae_Bot2011_COI	S17-03	1	BC77283_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77283	Sample	BC77283	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-03	COI	45	BC77283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-03	ATGAACAGTCTATCCTCCTTTAG	138.48586
BC77285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77779	WGS84	BC77285	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77285_Aamu_Bax2006_COI	S17-05	1	BC77285_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77285	Sample	BC77285	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-05	COI	45	BC77285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-05	GCACAACCGGGATCTTTACTTC	138.51794
BC77285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77779	WGS84	BC77285	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77285_Pcan_Bot2011_IGS	S17-05	1	BC77285_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77285	Sample	BC77285	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-05	IGS	45	BC77285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-05	CGTAATCCTCAGTACTGGCTA	138.51794
BC77285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77779	WGS84	BC77285	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77285_Sspa_Oph2007_COI	S17-05	1	BC77285_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77285	Sample	BC77285	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-05	COI	45	BC77285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-05	GAAGGCCTTCCGATGATC	138.51794
BC77285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77779	WGS84	BC77285	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77285_Upin_Bot2011_COI	S17-05	1	BC77285_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77285	Sample	BC77285	targeted	Absent	Sample	Upin_Bot2011_COI	S17-05	COI	45	BC77285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.51794
BC77286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77881	WGS84	BC77286	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77286_Cjap_Sim2018_COI	S17-06	1	BC77286_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77286	Sample	BC77286	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-06	COI	45	BC77286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51774
BC77286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77881	WGS84	BC77286	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.67	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77286_Mgig_Bot2012_COI	S17-06	1	BC77286_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77286	Sample	BC77286	targeted	Present	Sample	Mgig_Bot2012_COI	S17-06	COI	45	BC77286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.51774
BC77287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80962	WGS84	BC77287	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77287_Cmae_Bot2011_COI	S17-07	1	BC77287_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77287	Sample	BC77287	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-07	COI	45	BC77287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-07	ATGAACAGTCTATCCTCCTTTAG	138.51817
BC77289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81059	WGS84	BC77289	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77289_Aamu_Bax2006_COI	S17-09	1	BC77289_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77289	Sample	BC77289	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-09	COI	45	BC77289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-09	GCACAACCGGGATCTTTACTTC	138.52046
BC77289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81059	WGS84	BC77289	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77289_Pcan_Bot2011_IGS	S17-09	1	BC77289_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77289	Sample	BC77289	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-09	IGS	45	BC77289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-09	CGTAATCCTCAGTACTGGCTA	138.52046
BC77289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81059	WGS84	BC77289	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77289_Sspa_Oph2007_COI	S17-09	1	BC77289_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77289	Sample	BC77289	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-09	COI	45	BC77289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-09	GAAGGCCTTCCGATGATC	138.52046
BC77289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81059	WGS84	BC77289	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77289_Upin_Bot2011_COI	S17-09	1	BC77289_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77289	Sample	BC77289	targeted	Absent	Sample	Upin_Bot2011_COI	S17-09	COI	45	BC77289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.52046
BC77290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84498	WGS84	BC77290	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77290_Cmae_Bot2011_COI	S17-10	1	BC77290_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77290	Sample	BC77290	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-10	COI	45	BC77290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-10	ATGAACAGTCTATCCTCCTTTAG	138.49722
BC77292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84305	WGS84	BC77292	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77292_Aamu_Bax2006_COI	S17-12	1	BC77292_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77292	Sample	BC77292	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-12	COI	45	BC77292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-12	GCACAACCGGGATCTTTACTTC	138.49884
BC77292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84305	WGS84	BC77292	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77292_Pcan_Bot2011_IGS	S17-12	1	BC77292_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77292	Sample	BC77292	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-12	IGS	45	BC77292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-12	CGTAATCCTCAGTACTGGCTA	138.49884
BC77292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84305	WGS84	BC77292	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77292_Sspa_Oph2007_COI	S17-12	1	BC77292_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77292	Sample	BC77292	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-12	COI	45	BC77292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-12	GAAGGCCTTCCGATGATC	138.49884
BC77292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84305	WGS84	BC77292	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77292_Upin_Bot2011_COI	S17-12	1	BC77292_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77292	Sample	BC77292	targeted	Absent	Sample	Upin_Bot2011_COI	S17-12	COI	45	BC77292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.49884
BC77293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8367	WGS84	BC77293	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77293_Cjap_Sim2018_COI	S17-13	1	BC77293_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77293	Sample	BC77293	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-13	COI	45	BC77293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50883
BC77293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8367	WGS84	BC77293	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77293_Mgig_Bot2012_COI	S17-13	1	BC77293_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77293	Sample	BC77293	targeted	Absent	Sample	Mgig_Bot2012_COI	S17-13	COI	45	BC77293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.50883
BC77294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83721	WGS84	BC77294	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77294_Cmae_Bot2011_COI	S17-14	1	BC77294_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77294	Sample	BC77294	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-14	COI	45	BC77294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-14	ATGAACAGTCTATCCTCCTTTAG	138.51088
BC77296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83556	WGS84	BC77296	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77296_Aamu_Bax2006_COI	S18-01	1	BC77296_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77296	Sample	BC77296	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-01	COI	45	BC77296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-01	GCACAACCGGGATCTTTACTTC	138.50784
BC77296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83556	WGS84	BC77296	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77296_Pcan_Bot2011_IGS	S18-01	1	BC77296_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77296	Sample	BC77296	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-01	IGS	45	BC77296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-01	CGTAATCCTCAGTACTGGCTA	138.50784
BC77296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83556	WGS84	BC77296	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.459	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77296_Sspa_Oph2007_COI	S18-01	1	BC77296_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77296	Sample	BC77296	targeted	Present	Sample	Sspa_Oph2007_COI	S18-01	COI	45	BC77296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-01	GAAGGCCTTCCGATGATC	138.50784
BC77296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83556	WGS84	BC77296	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77296_Upin_Bot2011_COI	S18-01	1	BC77296_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77296	Sample	BC77296	targeted	Absent	Sample	Upin_Bot2011_COI	S18-01	COI	45	BC77296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.50784
BC77297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83622	WGS84	BC77297	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77297_Cjap_Sim2018_COI	S18-02	1	BC77297_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77297	Sample	BC77297	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-02	COI	45	BC77297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50888
BC77297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83622	WGS84	BC77297	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.093	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77297_Mgig_Bot2012_COI	S18-02	1	BC77297_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77297	Sample	BC77297	targeted	Present	Sample	Mgig_Bot2012_COI	S18-02	COI	45	BC77297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.50888
BC77298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83661	WGS84	BC77298	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77298_Cmae_Bot2011_COI	S18-03	1	BC77298_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77298	Sample	BC77298	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-03	COI	45	BC77298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-03	ATGAACAGTCTATCCTCCTTTAG	138.5099
BC77300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83734	WGS84	BC77300	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77300_Aamu_Bax2006_COI	S18-05	1	BC77300_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77300	Sample	BC77300	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-05	COI	45	BC77300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-05	GCACAACCGGGATCTTTACTTC	138.51042
BC77300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83734	WGS84	BC77300	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77300_Pcan_Bot2011_IGS	S18-05	1	BC77300_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77300	Sample	BC77300	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-05	IGS	45	BC77300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-05	CGTAATCCTCAGTACTGGCTA	138.51042
BC77300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83734	WGS84	BC77300	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.785	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77300_Sspa_Oph2007_COI	S18-05	1	BC77300_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77300	Sample	BC77300	targeted	Present	Sample	Sspa_Oph2007_COI	S18-05	COI	45	BC77300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-05	GAAGGCCTTCCGATGATC	138.51042
BC77300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83734	WGS84	BC77300	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77300_Upin_Bot2011_COI	S18-05	1	BC77300_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77300	Sample	BC77300	targeted	Absent	Sample	Upin_Bot2011_COI	S18-05	COI	45	BC77300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.51042
BC77301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80836	WGS84	BC77301	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77301_Cjap_Sim2018_COI	S18-06	1	BC77301_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77301	Sample	BC77301	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-06	COI	45	BC77301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51628
BC77301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80836	WGS84	BC77301	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.072	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77301_Mgig_Bot2012_COI	S18-06	1	BC77301_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77301	Sample	BC77301	targeted	Present	Sample	Mgig_Bot2012_COI	S18-06	COI	45	BC77301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.51628
BC77302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77302	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77302_Cmae_Bot2011_COI	S18-07	1	BC77302_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77302	Sample	BC77302	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-07	COI	45	BC77302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-07	ATGAACAGTCTATCCTCCTTTAG	138.51771
BC77304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81006	WGS84	BC77304	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77304_Aamu_Bax2006_COI	S18-09	1	BC77304_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77304	Sample	BC77304	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-09	COI	45	BC77304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-09	GCACAACCGGGATCTTTACTTC	138.51952
BC77304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81006	WGS84	BC77304	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77304_Pcan_Bot2011_IGS	S18-09	1	BC77304_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77304	Sample	BC77304	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-09	IGS	45	BC77304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-09	CGTAATCCTCAGTACTGGCTA	138.51952
BC77304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81006	WGS84	BC77304	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.389	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77304_Sspa_Oph2007_COI	S18-09	1	BC77304_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77304	Sample	BC77304	targeted	Present	Sample	Sspa_Oph2007_COI	S18-09	COI	45	BC77304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-09	GAAGGCCTTCCGATGATC	138.51952
BC77304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81006	WGS84	BC77304	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77304_Upin_Bot2011_COI	S18-09	1	BC77304_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77304	Sample	BC77304	targeted	Absent	Sample	Upin_Bot2011_COI	S18-09	COI	45	BC77304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.51952
BC77305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77305	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77305_Cjap_Sim2018_COI	S18-10	1	BC77305_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77305	Sample	BC77305	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-10	COI	45	BC77305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5198
BC77305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77305	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.281	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77305_Mgig_Bot2012_COI	S18-10	1	BC77305_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77305	Sample	BC77305	targeted	Present	Sample	Mgig_Bot2012_COI	S18-10	COI	45	BC77305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.5198
BC77306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77317	WGS84	BC77306	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77306_Cmae_Bot2011_COI	S19-01	1	BC77306_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77306	Sample	BC77306	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-01	COI	45	BC77306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-01	ATGAACAGTCTATCCTCCTTTAG	138.48557
BC77308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77295	WGS84	BC77308	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77308_Aamu_Bax2006_COI	S19-03	1	BC77308_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77308	Sample	BC77308	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-03	COI	45	BC77308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-03	GCACAACCGGGATCTTTACTTC	138.48449
BC77308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77295	WGS84	BC77308	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77308_Pcan_Bot2011_IGS	S19-03	1	BC77308_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77308	Sample	BC77308	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-03	IGS	45	BC77308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-03	CGTAATCCTCAGTACTGGCTA	138.48449
BC77308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77295	WGS84	BC77308	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.51	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77308_Sspa_Oph2007_COI	S19-03	1	BC77308_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77308	Sample	BC77308	targeted	Present	Sample	Sspa_Oph2007_COI	S19-03	COI	45	BC77308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-03	GAAGGCCTTCCGATGATC	138.48449
BC77308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77295	WGS84	BC77308	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77308_Upin_Bot2011_COI	S19-03	1	BC77308_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77308	Sample	BC77308	targeted	Absent	Sample	Upin_Bot2011_COI	S19-03	COI	45	BC77308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.48449
BC77309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77781	WGS84	BC77309	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77309_Cjap_Sim2018_COI	S19-04	1	BC77309_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77309	Sample	BC77309	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-04	COI	45	BC77309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51905
BC77309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77781	WGS84	BC77309	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.991	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77309_Mgig_Bot2012_COI	S19-04	1	BC77309_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77309	Sample	BC77309	targeted	Present	Sample	Mgig_Bot2012_COI	S19-04	COI	45	BC77309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.51905
BC77311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78118	WGS84	BC77311	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77311_Aamu_Bax2006_COI	S19-06	1	BC77311_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77311	Sample	BC77311	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-06	COI	45	BC77311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-06	GCACAACCGGGATCTTTACTTC	138.5172
BC77311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78118	WGS84	BC77311	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77311_Pcan_Bot2011_IGS	S19-06	1	BC77311_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77311	Sample	BC77311	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-06	IGS	45	BC77311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-06	CGTAATCCTCAGTACTGGCTA	138.5172
BC77311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78118	WGS84	BC77311	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.146	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77311_Sspa_Oph2007_COI	S19-06	1	BC77311_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77311	Sample	BC77311	targeted	Present	Sample	Sspa_Oph2007_COI	S19-06	COI	45	BC77311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-06	GAAGGCCTTCCGATGATC	138.5172
BC77311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78118	WGS84	BC77311	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77311_Upin_Bot2011_COI	S19-06	1	BC77311_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77311	Sample	BC77311	targeted	Absent	Sample	Upin_Bot2011_COI	S19-06	COI	45	BC77311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.5172
BC77312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84678	WGS84	BC77312	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77312_Cjap_Sim2018_COI	S19-07	1	BC77312_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77312	Sample	BC77312	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-07	COI	45	BC77312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49755
BC77312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84678	WGS84	BC77312	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77312_Mgig_Bot2012_COI	S19-07	1	BC77312_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77312	Sample	BC77312	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-07	COI	45	BC77312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.49755
BC77313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84552	WGS84	BC77313	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77313_Cmae_Bot2011_COI	S19-08	1	BC77313_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77313	Sample	BC77313	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-08	COI	45	BC77313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-08	ATGAACAGTCTATCCTCCTTTAG	138.49703
BC77315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83658	WGS84	BC77315	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77315_Aamu_Bax2006_COI	S19-10	1	BC77315_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77315	Sample	BC77315	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-10	COI	45	BC77315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-10	GCACAACCGGGATCTTTACTTC	138.50835
BC77315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83658	WGS84	BC77315	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77315_Pcan_Bot2011_IGS	S19-10	1	BC77315_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77315	Sample	BC77315	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-10	IGS	45	BC77315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-10	CGTAATCCTCAGTACTGGCTA	138.50835
BC77315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83658	WGS84	BC77315	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.561	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77315_Sspa_Oph2007_COI	S19-10	1	BC77315_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77315	Sample	BC77315	targeted	Present	Sample	Sspa_Oph2007_COI	S19-10	COI	45	BC77315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-10	GAAGGCCTTCCGATGATC	138.50835
BC77315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83658	WGS84	BC77315	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77315_Upin_Bot2011_COI	S19-10	1	BC77315_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77315	Sample	BC77315	targeted	Absent	Sample	Upin_Bot2011_COI	S19-10	COI	45	BC77315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.50835
BC77316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83711	WGS84	BC77316	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77316_Cjap_Sim2018_COI	S19-11	1	BC77316_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77316	Sample	BC77316	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-11	COI	45	BC77316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50972
BC77316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83711	WGS84	BC77316	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77316_Mgig_Bot2012_COI	S19-11	1	BC77316_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77316	Sample	BC77316	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-11	COI	45	BC77316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.50972
BC77317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83647	WGS84	BC77317	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77317_Cmae_Bot2011_COI	S19-12	1	BC77317_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77317	Sample	BC77317	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-12	COI	45	BC77317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-12	ATGAACAGTCTATCCTCCTTTAG	138.50948
BC77319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81009	WGS84	BC77319	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77319_Aamu_Bax2006_COI	S19-14	1	BC77319_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77319	Sample	BC77319	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-14	COI	45	BC77319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-14	GCACAACCGGGATCTTTACTTC	138.52
BC77319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81009	WGS84	BC77319	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77319_Pcan_Bot2011_IGS	S19-14	1	BC77319_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77319	Sample	BC77319	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-14	IGS	45	BC77319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-14	CGTAATCCTCAGTACTGGCTA	138.52
BC77319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81009	WGS84	BC77319	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.22	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77319_Sspa_Oph2007_COI	S19-14	1	BC77319_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77319	Sample	BC77319	targeted	Present	Sample	Sspa_Oph2007_COI	S19-14	COI	45	BC77319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-14	GAAGGCCTTCCGATGATC	138.52
BC77319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81009	WGS84	BC77319	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77319_Upin_Bot2011_COI	S19-14	1	BC77319_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77319	Sample	BC77319	targeted	Absent	Sample	Upin_Bot2011_COI	S19-14	COI	45	BC77319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.52
BC77320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81046	WGS84	BC77320	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77320_Cmae_Bot2011_COI	S19-15	1	BC77320_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77320	Sample	BC77320	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-15	COI	45	BC77320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-15	ATGAACAGTCTATCCTCCTTTAG	138.52139
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28890_Cjap_Sim2018_COI	POR-B29	1	BD28890_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28890_Esin_And2018_Cyb	POR-B29	1	BD28890_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B29	Cytb	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	ACCCCTCCTCATATCCAACCA	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28890_Mgig_Bot2012_COI	POR-B29	1	BD28890_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	TCTTATTCGTTGGAGACTTTATAACCCT	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28890_Mjap_Gib2022_COI	POR-B29	1	BD28890_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	AATTTAATTGTGACTAGGCATGGTCTG	141.62107
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28891_Cmae_Bot2011_COI	POR-B30	1	BD28891_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	ATGAACAGTCTATCCTCCTTTAG	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28891_Hsan_Wil2021_COI	POR-B30	1	BD28891_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	CCTGGGCCGGTATAGTAGGT	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28891_Mros_Gib2022_COI	POR-B30	1	BD28891_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28891_Msal_Bot2012_COI	POR-B30	1	BD28891_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	GYTAGTTCCRATGATGTTAGCTG	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28891_Mstr_Wil2021_COI	POR-B30	1	BD28891_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	GTAGTGGTAACTACTCATGCTTTA	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28891_Pvir_Dia2013_COI	POR-B30	1	BD28891_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	CTTAGTGGCATTAATTCGDAATCC	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28891_Rhar_Sim2018_COI	POR-B30	1	BD28891_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	CTGCTGCTATTGCTCATG	141.62277
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28893_Aamu_Bax2006_COI	POR-B32	1	BD28893_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	GCACAACCGGGATCTTTACTTC	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28893_Pcan_Bot2011_IGS	POR-B32	1	BD28893_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B32	IGS	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	CGTAATCCTCAGTACTGGCTA	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28893_Pper_Dia2013_COI	POR-B32	1	BD28893_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	CTTAGTGGCATTAATTCGDAATCC	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28893_Sspa_Oph2007_COI	POR-B32	1	BD28893_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	GAAGGCCTTCCGATGATC	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28893_Upin_Bot2011_COI	POR-B32	1	BD28893_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	CTTTAATTACAGCGTTTTTATTGTTGT	141.62082
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28894_Cjap_Sim2018_COI	POR-B33	1	BD28894_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28894_Esin_And2018_Cyb	POR-B33	1	BD28894_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B33	Cytb	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	ACCCCTCCTCATATCCAACCA	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28894_Mgig_Bot2012_COI	POR-B33	1	BD28894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	TCTTATTCGTTGGAGACTTTATAACCCT	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28894_Mjap_Gib2022_COI	POR-B33	1	BD28894_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	AATTTAATTGTGACTAGGCATGGTCTG	141.61708
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28895_Cmae_Bot2011_COI	POR-B34	1	BD28895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	ATGAACAGTCTATCCTCCTTTAG	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28895_Hsan_Wil2021_COI	POR-B34	1	BD28895_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	CCTGGGCCGGTATAGTAGGT	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.836	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28895_Mros_Gib2022_COI	POR-B34	1	BD28895_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Present	Sample	Mros_Gib2022_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28895_Msal_Bot2012_COI	POR-B34	1	BD28895_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	GYTAGTTCCRATGATGTTAGCTG	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28895_Mstr_Wil2021_COI	POR-B34	1	BD28895_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	GTAGTGGTAACTACTCATGCTTTA	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28895_Pvir_Dia2013_COI	POR-B34	1	BD28895_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	CTTAGTGGCATTAATTCGDAATCC	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28895_Rhar_Sim2018_COI	POR-B34	1	BD28895_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	CTGCTGCTATTGCTCATG	141.61843
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28897_Aamu_Bax2006_COI	POR-B21	1	BD28897_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	GCACAACCGGGATCTTTACTTC	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28897_Pcan_Bot2011_IGS	POR-B21	1	BD28897_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B21	IGS	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	CGTAATCCTCAGTACTGGCTA	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28897_Pper_Dia2013_COI	POR-B21	1	BD28897_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	CTTAGTGGCATTAATTCGDAATCC	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28897_Sspa_Oph2007_COI	POR-B21	1	BD28897_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	GAAGGCCTTCCGATGATC	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28897_Upin_Bot2011_COI	POR-B21	1	BD28897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	CTTTAATTACAGCGTTTTTATTGTTGT	141.62176
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28898_Cjap_Sim2018_COI	POR-B20	1	BD28898_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28898_Esin_And2018_Cyb	POR-B20	1	BD28898_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B20	Cytb	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	ACCCCTCCTCATATCCAACCA	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28898_Mjap_Gib2022_COI	POR-B20	1	BD28898_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	AATTTAATTGTGACTAGGCATGGTCTG	141.62028
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28899_Cmae_Bot2011_COI	POR-B19	1	BD28899_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	ATGAACAGTCTATCCTCCTTTAG	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28899_Hsan_Wil2021_COI	POR-B19	1	BD28899_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	CCTGGGCCGGTATAGTAGGT	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28899_Mros_Gib2022_COI	POR-B19	1	BD28899_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28899_Msal_Bot2012_COI	POR-B19	1	BD28899_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	GYTAGTTCCRATGATGTTAGCTG	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28899_Mstr_Wil2021_COI	POR-B19	1	BD28899_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	GTAGTGGTAACTACTCATGCTTTA	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28899_Pvir_Dia2013_COI	POR-B19	1	BD28899_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	CTTAGTGGCATTAATTCGDAATCC	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28899_Rhar_Sim2018_COI	POR-B19	1	BD28899_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	CTGCTGCTATTGCTCATG	141.6189
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28901_Aamu_Bax2006_COI	POR-B17	1	BD28901_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	GCACAACCGGGATCTTTACTTC	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28901_Pcan_Bot2011_IGS	POR-B17	1	BD28901_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B17	IGS	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	CGTAATCCTCAGTACTGGCTA	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28901_Pper_Dia2013_COI	POR-B17	1	BD28901_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	CTTAGTGGCATTAATTCGDAATCC	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28901_Sspa_Oph2007_COI	POR-B17	1	BD28901_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	GAAGGCCTTCCGATGATC	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.244	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28901_Upin_Bot2011_COI	POR-B17	1	BD28901_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Present	Sample	Upin_Bot2011_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	CTTTAATTACAGCGTTTTTATTGTTGT	141.61616
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28902_Cjap_Sim2018_COI	POR-B16	1	BD28902_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28902_Esin_And2018_Cyb	POR-B16	1	BD28902_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B16	Cytb	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	ACCCCTCCTCATATCCAACCA	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28902_Mgig_Bot2012_COI	POR-B16	1	BD28902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	TCTTATTCGTTGGAGACTTTATAACCCT	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28902_Mjap_Gib2022_COI	POR-B16	1	BD28902_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	AATTTAATTGTGACTAGGCATGGTCTG	141.6148
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28903_Cmae_Bot2011_COI	POR-B15	1	BD28903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	ATGAACAGTCTATCCTCCTTTAG	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28903_Hsan_Wil2021_COI	POR-B15	1	BD28903_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	CCTGGGCCGGTATAGTAGGT	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28903_Mros_Gib2022_COI	POR-B15	1	BD28903_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28903_Msal_Bot2012_COI	POR-B15	1	BD28903_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	GYTAGTTCCRATGATGTTAGCTG	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28903_Mstr_Wil2021_COI	POR-B15	1	BD28903_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	GTAGTGGTAACTACTCATGCTTTA	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28903_Pvir_Dia2013_COI	POR-B15	1	BD28903_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	CTTAGTGGCATTAATTCGDAATCC	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28903_Rhar_Sim2018_COI	POR-B15	1	BD28903_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	CTGCTGCTATTGCTCATG	141.61341
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28905_Aamu_Bax2006_COI	POR-B13	1	BD28905_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	GCACAACCGGGATCTTTACTTC	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28905_Pcan_Bot2011_IGS	POR-B13	1	BD28905_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B13	IGS	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	CGTAATCCTCAGTACTGGCTA	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28905_Pper_Dia2013_COI	POR-B13	1	BD28905_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	CTTAGTGGCATTAATTCGDAATCC	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28905_Sspa_Oph2007_COI	POR-B13	1	BD28905_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	GAAGGCCTTCCGATGATC	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28905_Upin_Bot2011_COI	POR-B13	1	BD28905_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	CTTTAATTACAGCGTTTTTATTGTTGT	141.61265
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28906_Cjap_Sim2018_COI	POR-B12	1	BD28906_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28906_Esin_And2018_Cyb	POR-B12	1	BD28906_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B12	Cytb	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	ACCCCTCCTCATATCCAACCA	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28906_Mgig_Bot2012_COI	POR-B12	1	BD28906_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	TCTTATTCGTTGGAGACTTTATAACCCT	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28906_Mjap_Gib2022_COI	POR-B12	1	BD28906_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	AATTTAATTGTGACTAGGCATGGTCTG	141.61129
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28907_Cmae_Bot2011_COI	POR-B11	1	BD28907_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	ATGAACAGTCTATCCTCCTTTAG	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28907_Hsan_Wil2021_COI	POR-B11	1	BD28907_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	CCTGGGCCGGTATAGTAGGT	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28907_Mros_Gib2022_COI	POR-B11	1	BD28907_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28907_Msal_Bot2012_COI	POR-B11	1	BD28907_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	GYTAGTTCCRATGATGTTAGCTG	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28907_Mstr_Wil2021_COI	POR-B11	1	BD28907_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	GTAGTGGTAACTACTCATGCTTTA	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28907_Pvir_Dia2013_COI	POR-B11	1	BD28907_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	CTTAGTGGCATTAATTCGDAATCC	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28907_Rhar_Sim2018_COI	POR-B11	1	BD28907_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	CTGCTGCTATTGCTCATG	141.62078
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28909_Aamu_Bax2006_COI	POR-B10	1	BD28909_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	GCACAACCGGGATCTTTACTTC	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28909_Pcan_Bot2011_IGS	POR-B10	1	BD28909_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B10	IGS	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	CGTAATCCTCAGTACTGGCTA	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28909_Pper_Dia2013_COI	POR-B10	1	BD28909_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	CTTAGTGGCATTAATTCGDAATCC	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28909_Sspa_Oph2007_COI	POR-B10	1	BD28909_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	GAAGGCCTTCCGATGATC	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28909_Upin_Bot2011_COI	POR-B10	1	BD28909_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	CTTTAATTACAGCGTTTTTATTGTTGT	141.62026
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28910_Cmae_Bot2011_COI	POR-B09	1	BD28910_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	ATGAACAGTCTATCCTCCTTTAG	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28910_Hsan_Wil2021_COI	POR-B09	1	BD28910_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	CCTGGGCCGGTATAGTAGGT	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.803	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28910_Mros_Gib2022_COI	POR-B09	1	BD28910_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Present	Sample	Mros_Gib2022_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28910_Msal_Bot2012_COI	POR-B09	1	BD28910_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	GYTAGTTCCRATGATGTTAGCTG	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28910_Mstr_Wil2021_COI	POR-B09	1	BD28910_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	GTAGTGGTAACTACTCATGCTTTA	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28910_Pvir_Dia2013_COI	POR-B09	1	BD28910_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	CTTAGTGGCATTAATTCGDAATCC	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28910_Rhar_Sim2018_COI	POR-B09	1	BD28910_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	CTGCTGCTATTGCTCATG	141.61892
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28912_Aamu_Bax2006_COI	POR-B07	1	BD28912_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	GCACAACCGGGATCTTTACTTC	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28912_Pcan_Bot2011_IGS	POR-B07	1	BD28912_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B07	IGS	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	CGTAATCCTCAGTACTGGCTA	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28912_Pper_Dia2013_COI	POR-B07	1	BD28912_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	CTTAGTGGCATTAATTCGDAATCC	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28912_Sspa_Oph2007_COI	POR-B07	1	BD28912_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	GAAGGCCTTCCGATGATC	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.574	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28912_Upin_Bot2011_COI	POR-B07	1	BD28912_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Present	Sample	Upin_Bot2011_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	CTTTAATTACAGCGTTTTTATTGTTGT	141.61615
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28913_Cjap_Sim2018_COI	POR-B06	1	BD28913_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28913_Esin_And2018_Cyb	POR-B06	1	BD28913_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B06	Cytb	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	ACCCCTCCTCATATCCAACCA	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28913_Mgig_Bot2012_COI	POR-B06	1	BD28913_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	TCTTATTCGTTGGAGACTTTATAACCCT	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28913_Mjap_Gib2022_COI	POR-B06	1	BD28913_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	AATTTAATTGTGACTAGGCATGGTCTG	141.61473
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28914_Cmae_Bot2011_COI	POR-B05	1	BD28914_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	ATGAACAGTCTATCCTCCTTTAG	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28914_Hsan_Wil2021_COI	POR-B05	1	BD28914_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	CCTGGGCCGGTATAGTAGGT	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.533	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28914_Mros_Gib2022_COI	POR-B05	1	BD28914_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Present	Sample	Mros_Gib2022_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28914_Msal_Bot2012_COI	POR-B05	1	BD28914_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	GYTAGTTCCRATGATGTTAGCTG	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28914_Mstr_Wil2021_COI	POR-B05	1	BD28914_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	GTAGTGGTAACTACTCATGCTTTA	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28914_Pvir_Dia2013_COI	POR-B05	1	BD28914_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	CTTAGTGGCATTAATTCGDAATCC	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28914_Rhar_Sim2018_COI	POR-B05	1	BD28914_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	CTGCTGCTATTGCTCATG	141.61197
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28916_Aamu_Bax2006_COI	POR-B03	1	BD28916_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	GCACAACCGGGATCTTTACTTC	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28916_Pcan_Bot2011_IGS	POR-B03	1	BD28916_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B03	IGS	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	CGTAATCCTCAGTACTGGCTA	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28916_Pper_Dia2013_COI	POR-B03	1	BD28916_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	CTTAGTGGCATTAATTCGDAATCC	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28916_Sspa_Oph2007_COI	POR-B03	1	BD28916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	GAAGGCCTTCCGATGATC	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28916_Upin_Bot2011_COI	POR-B03	1	BD28916_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	CTTTAATTACAGCGTTTTTATTGTTGT	141.61129
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28917_Cjap_Sim2018_COI	POR-B02	1	BD28917_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28917_Esin_And2018_Cyb	POR-B02	1	BD28917_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B02	Cytb	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	ACCCCTCCTCATATCCAACCA	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28917_Mgig_Bot2012_COI	POR-B02	1	BD28917_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	TCTTATTCGTTGGAGACTTTATAACCCT	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28917_Mjap_Gib2022_COI	POR-B02	1	BD28917_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	AATTTAATTGTGACTAGGCATGGTCTG	141.61065
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28918_Cmae_Bot2011_COI	POR-B01	1	BD28918_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	ATGAACAGTCTATCCTCCTTTAG	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28918_Hsan_Wil2021_COI	POR-B01	1	BD28918_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	CCTGGGCCGGTATAGTAGGT	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28918_Mros_Gib2022_COI	POR-B01	1	BD28918_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28918_Msal_Bot2012_COI	POR-B01	1	BD28918_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	GYTAGTTCCRATGATGTTAGCTG	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28918_Mstr_Wil2021_COI	POR-B01	1	BD28918_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	GTAGTGGTAACTACTCATGCTTTA	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28918_Pvir_Dia2013_COI	POR-B01	1	BD28918_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	CTTAGTGGCATTAATTCGDAATCC	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28918_Rhar_Sim2018_COI	POR-B01	1	BD28918_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	CTGCTGCTATTGCTCATG	141.60984
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81218	WGS84	BD28920	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28920_Asen_Wil2025_COI	ADL-B34	1	BD28920_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	GTTGTTGTTACAACACATGCTCTTTTG	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28920_Cjap_Sim2018_COI	ADL-B34	1	BD28920_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28920_Esin_And2018_Cyb	ADL-B34	1	BD28920_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B34	Cytb	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	ACCCCTCCTCATATCCAACCA	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.739	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28920_Mgig_Bot2012_COI	ADL-B34	1	BD28920_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	TCTTATTCGTTGGAGACTTTATAACCCT	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28920_Mjap_Gib2022_COI	ADL-B34	1	BD28920_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	AATTTAATTGTGACTAGGCATGGTCTG	138.514
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28921_Cmae_Bot2011_COI	ADL-B33	1	BD28921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	ATGAACAGTCTATCCTCCTTTAG	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28921_Hsan_Wil2021_COI	ADL-B33	1	BD28921_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	CCTGGGCCGGTATAGTAGGT	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28921_Mros_Gib2022_COI	ADL-B33	1	BD28921_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28921_Msal_Bot2012_COI	ADL-B33	1	BD28921_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	GYTAGTTCCRATGATGTTAGCTG	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28921_Mstr_Wil2021_COI	ADL-B33	1	BD28921_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	GTAGTGGTAACTACTCATGCTTTA	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28921_Pvir_Dia2013_COI	ADL-B33	1	BD28921_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	CTTAGTGGCATTAATTCGDAATCC	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28921_Rhar_Sim2018_COI	ADL-B33	1	BD28921_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	CTGCTGCTATTGCTCATG	138.51399
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28923_Aamu_Bax2006_COI	ADL-B31	1	BD28923_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	GCACAACCGGGATCTTTACTTC	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28923_Pcan_Bot2011_IGS	ADL-B31	1	BD28923_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B31	IGS	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	CGTAATCCTCAGTACTGGCTA	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28923_Pper_Dia2013_COI	ADL-B31	1	BD28923_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	CTTAGTGGCATTAATTCGDAATCC	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28923_Sspa_Oph2007_COI	ADL-B31	1	BD28923_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	GAAGGCCTTCCGATGATC	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28923_Upin_Bot2011_COI	ADL-B31	1	BD28923_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	CTTTAATTACAGCGTTTTTATTGTTGT	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81627	WGS84	BD28923	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28923_Vgib_Wil2025_COI	ADL-B31	1	BD28923_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	CCCCTCTTTACAAGTTCTTTAGGGT	138.51405
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28924_Cjap_Sim2018_COI	ADL-B30	1	BD28924_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28924_Esin_And2018_Cyb	ADL-B30	1	BD28924_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B30	Cytb	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	ACCCCTCCTCATATCCAACCA	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.395	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28924_Mgig_Bot2012_COI	ADL-B30	1	BD28924_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	TCTTATTCGTTGGAGACTTTATAACCCT	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28924_Mjap_Gib2022_COI	ADL-B30	1	BD28924_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	AATTTAATTGTGACTAGGCATGGTCTG	138.51328
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28925_Cmae_Bot2011_COI	ADL-B29	1	BD28925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	ATGAACAGTCTATCCTCCTTTAG	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28925_Hsan_Wil2021_COI	ADL-B29	1	BD28925_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	CCTGGGCCGGTATAGTAGGT	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28925_Mros_Gib2022_COI	ADL-B29	1	BD28925_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28925_Msal_Bot2012_COI	ADL-B29	1	BD28925_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	GYTAGTTCCRATGATGTTAGCTG	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28925_Mstr_Wil2021_COI	ADL-B29	1	BD28925_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	GTAGTGGTAACTACTCATGCTTTA	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28925_Pvir_Dia2013_COI	ADL-B29	1	BD28925_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	CTTAGTGGCATTAATTCGDAATCC	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28925_Rhar_Sim2018_COI	ADL-B29	1	BD28925_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	CTGCTGCTATTGCTCATG	138.51263
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28927_Aamu_Bax2006_COI	ADL-B27	1	BD28927_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	GCACAACCGGGATCTTTACTTC	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28927_Pcan_Bot2011_IGS	ADL-B27	1	BD28927_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B27	IGS	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	CGTAATCCTCAGTACTGGCTA	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28927_Pper_Dia2013_COI	ADL-B27	1	BD28927_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	CTTAGTGGCATTAATTCGDAATCC	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28927_Sspa_Oph2007_COI	ADL-B27	1	BD28927_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	GAAGGCCTTCCGATGATC	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28927_Upin_Bot2011_COI	ADL-B27	1	BD28927_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	CTTTAATTACAGCGTTTTTATTGTTGT	138.51146
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28928_Cjap_Sim2018_COI	ADL-B26	1	BD28928_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28928_Esin_And2018_Cyb	ADL-B26	1	BD28928_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B26	Cytb	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	ACCCCTCCTCATATCCAACCA	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.464	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28928_Mgig_Bot2012_COI	ADL-B26	1	BD28928_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	TCTTATTCGTTGGAGACTTTATAACCCT	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28928_Mjap_Gib2022_COI	ADL-B26	1	BD28928_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	AATTTAATTGTGACTAGGCATGGTCTG	138.51074
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28929_Cmae_Bot2011_COI	ADL-B25	1	BD28929_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	ATGAACAGTCTATCCTCCTTTAG	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28929_Hsan_Wil2021_COI	ADL-B25	1	BD28929_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	CCTGGGCCGGTATAGTAGGT	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28929_Mros_Gib2022_COI	ADL-B25	1	BD28929_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28929_Msal_Bot2012_COI	ADL-B25	1	BD28929_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	GYTAGTTCCRATGATGTTAGCTG	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28929_Mstr_Wil2021_COI	ADL-B25	1	BD28929_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	GTAGTGGTAACTACTCATGCTTTA	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28929_Pvir_Dia2013_COI	ADL-B25	1	BD28929_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	CTTAGTGGCATTAATTCGDAATCC	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28929_Rhar_Sim2018_COI	ADL-B25	1	BD28929_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	CTGCTGCTATTGCTCATG	138.51172
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28930_Aamu_Bax2006_COI	ADL-B24	1	BD28930_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	GCACAACCGGGATCTTTACTTC	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28930_Pcan_Bot2011_IGS	ADL-B24	1	BD28930_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B24	IGS	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	CGTAATCCTCAGTACTGGCTA	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28930_Pper_Dia2013_COI	ADL-B24	1	BD28930_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	CTTAGTGGCATTAATTCGDAATCC	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28930_Sspa_Oph2007_COI	ADL-B24	1	BD28930_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	GAAGGCCTTCCGATGATC	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28930_Upin_Bot2011_COI	ADL-B24	1	BD28930_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	CTTTAATTACAGCGTTTTTATTGTTGT	138.51033
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28931_Cjap_Sim2018_COI	ADL-B23	1	BD28931_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28931_Esin_And2018_Cyb	ADL-B23	1	BD28931_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B23	Cytb	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	ACCCCTCCTCATATCCAACCA	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.278	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28931_Mgig_Bot2012_COI	ADL-B23	1	BD28931_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	TCTTATTCGTTGGAGACTTTATAACCCT	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28931_Mjap_Gib2022_COI	ADL-B23	1	BD28931_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	AATTTAATTGTGACTAGGCATGGTCTG	138.51105
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28932_Cmae_Bot2011_COI	ADL-B22	1	BD28932_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	ATGAACAGTCTATCCTCCTTTAG	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28932_Hsan_Wil2021_COI	ADL-B22	1	BD28932_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	CCTGGGCCGGTATAGTAGGT	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28932_Mros_Gib2022_COI	ADL-B22	1	BD28932_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28932_Msal_Bot2012_COI	ADL-B22	1	BD28932_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	GYTAGTTCCRATGATGTTAGCTG	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28932_Mstr_Wil2021_COI	ADL-B22	1	BD28932_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	GTAGTGGTAACTACTCATGCTTTA	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28932_Pvir_Dia2013_COI	ADL-B22	1	BD28932_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	CTTAGTGGCATTAATTCGDAATCC	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28932_Rhar_Sim2018_COI	ADL-B22	1	BD28932_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	CTGCTGCTATTGCTCATG	138.50953
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28934_Aamu_Bax2006_COI	ADL-B20	1	BD28934_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	GCACAACCGGGATCTTTACTTC	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28934_Pcan_Bot2011_IGS	ADL-B20	1	BD28934_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B20	IGS	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	CGTAATCCTCAGTACTGGCTA	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28934_Pper_Dia2013_COI	ADL-B20	1	BD28934_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	CTTAGTGGCATTAATTCGDAATCC	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28934_Sspa_Oph2007_COI	ADL-B20	1	BD28934_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	GAAGGCCTTCCGATGATC	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28934_Upin_Bot2011_COI	ADL-B20	1	BD28934_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	CTTTAATTACAGCGTTTTTATTGTTGT	138.50884
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28935_Cjap_Sim2018_COI	ADL-B19	1	BD28935_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28935_Esin_And2018_Cyb	ADL-B19	1	BD28935_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B19	Cytb	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	ACCCCTCCTCATATCCAACCA	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.801	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28935_Mgig_Bot2012_COI	ADL-B19	1	BD28935_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	TCTTATTCGTTGGAGACTTTATAACCCT	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28935_Mjap_Gib2022_COI	ADL-B19	1	BD28935_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	AATTTAATTGTGACTAGGCATGGTCTG	138.50973
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28936_Cmae_Bot2011_COI	ADL-B18	1	BD28936_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	ATGAACAGTCTATCCTCCTTTAG	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28936_Hsan_Wil2021_COI	ADL-B18	1	BD28936_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	CCTGGGCCGGTATAGTAGGT	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28936_Mros_Gib2022_COI	ADL-B18	1	BD28936_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28936_Msal_Bot2012_COI	ADL-B18	1	BD28936_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	GYTAGTTCCRATGATGTTAGCTG	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28936_Mstr_Wil2021_COI	ADL-B18	1	BD28936_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	GTAGTGGTAACTACTCATGCTTTA	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28936_Pvir_Dia2013_COI	ADL-B18	1	BD28936_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	CTTAGTGGCATTAATTCGDAATCC	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28936_Rhar_Sim2018_COI	ADL-B18	1	BD28936_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	CTGCTGCTATTGCTCATG	138.50817
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28938_Aamu_Bax2006_COI	ADL-B16	1	BD28938_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	GCACAACCGGGATCTTTACTTC	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28938_Pcan_Bot2011_IGS	ADL-B16	1	BD28938_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B16	IGS	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	CGTAATCCTCAGTACTGGCTA	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28938_Pper_Dia2013_COI	ADL-B16	1	BD28938_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	CTTAGTGGCATTAATTCGDAATCC	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28938_Sspa_Oph2007_COI	ADL-B16	1	BD28938_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	GAAGGCCTTCCGATGATC	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28938_Upin_Bot2011_COI	ADL-B16	1	BD28938_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	CTTTAATTACAGCGTTTTTATTGTTGT	138.50756
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28939_Cjap_Sim2018_COI	ADL-B15	1	BD28939_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28939_Esin_And2018_Cyb	ADL-B15	1	BD28939_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B15	Cytb	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	ACCCCTCCTCATATCCAACCA	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.677	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28939_Mgig_Bot2012_COI	ADL-B15	1	BD28939_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	TCTTATTCGTTGGAGACTTTATAACCCT	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28939_Mjap_Gib2022_COI	ADL-B15	1	BD28939_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	AATTTAATTGTGACTAGGCATGGTCTG	138.50806
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28941_Aamu_Bax2006_COI	ADL-B13	1	BD28941_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	GCACAACCGGGATCTTTACTTC	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28941_Pcan_Bot2011_IGS	ADL-B13	1	BD28941_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B13	IGS	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	CGTAATCCTCAGTACTGGCTA	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28941_Pper_Dia2013_COI	ADL-B13	1	BD28941_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	CTTAGTGGCATTAATTCGDAATCC	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28941_Sspa_Oph2007_COI	ADL-B13	1	BD28941_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	GAAGGCCTTCCGATGATC	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28941_Upin_Bot2011_COI	ADL-B13	1	BD28941_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	CTTTAATTACAGCGTTTTTATTGTTGT	138.50754
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28942_Cjap_Sim2018_COI	ADL-B12	1	BD28942_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28942_Esin_And2018_Cyb	ADL-B12	1	BD28942_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B12	Cytb	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	ACCCCTCCTCATATCCAACCA	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.278	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28942_Mgig_Bot2012_COI	ADL-B12	1	BD28942_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	TCTTATTCGTTGGAGACTTTATAACCCT	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28942_Mjap_Gib2022_COI	ADL-B12	1	BD28942_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	AATTTAATTGTGACTAGGCATGGTCTG	138.5093
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28943_Cmae_Bot2011_COI	ADL-B11	1	BD28943_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	ATGAACAGTCTATCCTCCTTTAG	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28943_Hsan_Wil2021_COI	ADL-B11	1	BD28943_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	CCTGGGCCGGTATAGTAGGT	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28943_Mros_Gib2022_COI	ADL-B11	1	BD28943_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28943_Msal_Bot2012_COI	ADL-B11	1	BD28943_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	GYTAGTTCCRATGATGTTAGCTG	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28943_Mstr_Wil2021_COI	ADL-B11	1	BD28943_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	GTAGTGGTAACTACTCATGCTTTA	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28943_Pvir_Dia2013_COI	ADL-B11	1	BD28943_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	CTTAGTGGCATTAATTCGDAATCC	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28943_Rhar_Sim2018_COI	ADL-B11	1	BD28943_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	CTGCTGCTATTGCTCATG	138.50868
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28945_Aamu_Bax2006_COI	ADL-B09	1	BD28945_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	GCACAACCGGGATCTTTACTTC	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28945_Pcan_Bot2011_IGS	ADL-B09	1	BD28945_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B09	IGS	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	CGTAATCCTCAGTACTGGCTA	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28945_Pper_Dia2013_COI	ADL-B09	1	BD28945_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	CTTAGTGGCATTAATTCGDAATCC	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28945_Sspa_Oph2007_COI	ADL-B09	1	BD28945_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	GAAGGCCTTCCGATGATC	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28945_Upin_Bot2011_COI	ADL-B09	1	BD28945_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	CTTTAATTACAGCGTTTTTATTGTTGT	138.50754
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28946_Cjap_Sim2018_COI	ADL-B08	1	BD28946_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28946_Esin_And2018_Cyb	ADL-B08	1	BD28946_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B08	Cytb	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	ACCCCTCCTCATATCCAACCA	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.075	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28946_Mgig_Bot2012_COI	ADL-B08	1	BD28946_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28946_Mjap_Gib2022_COI	ADL-B08	1	BD28946_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	AATTTAATTGTGACTAGGCATGGTCTG	138.51028
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28947_Cmae_Bot2011_COI	ADL-B07	1	BD28947_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	ATGAACAGTCTATCCTCCTTTAG	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28947_Hsan_Wil2021_COI	ADL-B07	1	BD28947_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	CCTGGGCCGGTATAGTAGGT	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28947_Mros_Gib2022_COI	ADL-B07	1	BD28947_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28947_Msal_Bot2012_COI	ADL-B07	1	BD28947_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	GYTAGTTCCRATGATGTTAGCTG	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28947_Mstr_Wil2021_COI	ADL-B07	1	BD28947_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	GTAGTGGTAACTACTCATGCTTTA	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28947_Pvir_Dia2013_COI	ADL-B07	1	BD28947_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	CTTAGTGGCATTAATTCGDAATCC	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28947_Rhar_Sim2018_COI	ADL-B07	1	BD28947_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	CTGCTGCTATTGCTCATG	138.50757
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28949_Aamu_Bax2006_COI	ADL-B05	1	BD28949_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	GCACAACCGGGATCTTTACTTC	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28949_Pcan_Bot2011_IGS	ADL-B05	1	BD28949_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B05	IGS	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	CGTAATCCTCAGTACTGGCTA	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28949_Pper_Dia2013_COI	ADL-B05	1	BD28949_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	CTTAGTGGCATTAATTCGDAATCC	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28949_Sspa_Oph2007_COI	ADL-B05	1	BD28949_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	GAAGGCCTTCCGATGATC	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28949_Upin_Bot2011_COI	ADL-B05	1	BD28949_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	CTTTAATTACAGCGTTTTTATTGTTGT	138.50549
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28950_Cmae_Bot2011_COI	ADL-B04	1	BD28950_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	ATGAACAGTCTATCCTCCTTTAG	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28950_Hsan_Wil2021_COI	ADL-B04	1	BD28950_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	CCTGGGCCGGTATAGTAGGT	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28950_Mros_Gib2022_COI	ADL-B04	1	BD28950_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28950_Msal_Bot2012_COI	ADL-B04	1	BD28950_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	GYTAGTTCCRATGATGTTAGCTG	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28950_Mstr_Wil2021_COI	ADL-B04	1	BD28950_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	GTAGTGGTAACTACTCATGCTTTA	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28950_Pvir_Dia2013_COI	ADL-B04	1	BD28950_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	CTTAGTGGCATTAATTCGDAATCC	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28950_Rhar_Sim2018_COI	ADL-B04	1	BD28950_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	CTGCTGCTATTGCTCATG	138.50603
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28952_Aamu_Bax2006_COI	ADL-B02	1	BD28952_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	GCACAACCGGGATCTTTACTTC	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28952_Pcan_Bot2011_IGS	ADL-B02	1	BD28952_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B02	IGS	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	CGTAATCCTCAGTACTGGCTA	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28952_Pper_Dia2013_COI	ADL-B02	1	BD28952_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	CTTAGTGGCATTAATTCGDAATCC	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28952_Sspa_Oph2007_COI	ADL-B02	1	BD28952_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	GAAGGCCTTCCGATGATC	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28952_Upin_Bot2011_COI	ADL-B02	1	BD28952_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	CTTTAATTACAGCGTTTTTATTGTTGT	138.50066
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28953_Cjap_Sim2018_COI	ADL-B01	1	BD28953_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28953_Esin_And2018_Cyb	ADL-B01	1	BD28953_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B01	Cytb	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	ACCCCTCCTCATATCCAACCA	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28953_Mgig_Bot2012_COI	ADL-B01	1	BD28953_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	TCTTATTCGTTGGAGACTTTATAACCCT	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28953_Mjap_Gib2022_COI	ADL-B01	1	BD28953_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	AATTTAATTGTGACTAGGCATGGTCTG	138.49924
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28954_Cmae_Bot2011_COI	ADL-B70	1	BD28954_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	ATGAACAGTCTATCCTCCTTTAG	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28954_Hsan_Wil2021_COI	ADL-B70	1	BD28954_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	CCTGGGCCGGTATAGTAGGT	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28954_Mros_Gib2022_COI	ADL-B70	1	BD28954_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28954_Msal_Bot2012_COI	ADL-B70	1	BD28954_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	GYTAGTTCCRATGATGTTAGCTG	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28954_Mstr_Wil2021_COI	ADL-B70	1	BD28954_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	GTAGTGGTAACTACTCATGCTTTA	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28954_Pvir_Dia2013_COI	ADL-B70	1	BD28954_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	CTTAGTGGCATTAATTCGDAATCC	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28954_Rhar_Sim2018_COI	ADL-B70	1	BD28954_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	CTGCTGCTATTGCTCATG	138.5006
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28956_Aamu_Bax2006_COI	ADL-B68	1	BD28956_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	GCACAACCGGGATCTTTACTTC	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28956_Pcan_Bot2011_IGS	ADL-B68	1	BD28956_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B68	IGS	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	CGTAATCCTCAGTACTGGCTA	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28956_Pper_Dia2013_COI	ADL-B68	1	BD28956_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	CTTAGTGGCATTAATTCGDAATCC	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.331	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28956_Sspa_Oph2007_COI	ADL-B68	1	BD28956_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	GAAGGCCTTCCGATGATC	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28956_Upin_Bot2011_COI	ADL-B68	1	BD28956_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	CTTTAATTACAGCGTTTTTATTGTTGT	138.50173
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28957_Cjap_Sim2018_COI	ADL-B67	1	BD28957_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28957_Esin_And2018_Cyb	ADL-B67	1	BD28957_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B67	Cytb	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	ACCCCTCCTCATATCCAACCA	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.518	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28957_Mgig_Bot2012_COI	ADL-B67	1	BD28957_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	TCTTATTCGTTGGAGACTTTATAACCCT	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28957_Mjap_Gib2022_COI	ADL-B67	1	BD28957_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	AATTTAATTGTGACTAGGCATGGTCTG	138.49964
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28958_Cmae_Bot2011_COI	ADL-B66	1	BD28958_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	ATGAACAGTCTATCCTCCTTTAG	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28958_Hsan_Wil2021_COI	ADL-B66	1	BD28958_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	CCTGGGCCGGTATAGTAGGT	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28958_Mros_Gib2022_COI	ADL-B66	1	BD28958_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28958_Msal_Bot2012_COI	ADL-B66	1	BD28958_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	GYTAGTTCCRATGATGTTAGCTG	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28958_Mstr_Wil2021_COI	ADL-B66	1	BD28958_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	GTAGTGGTAACTACTCATGCTTTA	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28958_Pvir_Dia2013_COI	ADL-B66	1	BD28958_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	CTTAGTGGCATTAATTCGDAATCC	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28958_Rhar_Sim2018_COI	ADL-B66	1	BD28958_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	CTGCTGCTATTGCTCATG	138.49752
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28960_Cjap_Sim2018_COI	ADL-B64	1	BD28960_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28960_Esin_And2018_Cyb	ADL-B64	1	BD28960_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B64	Cytb	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	ACCCCTCCTCATATCCAACCA	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.029	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28960_Mgig_Bot2012_COI	ADL-B64	1	BD28960_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	TCTTATTCGTTGGAGACTTTATAACCCT	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28960_Mjap_Gib2022_COI	ADL-B64	1	BD28960_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	AATTTAATTGTGACTAGGCATGGTCTG	138.49652
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28961_Cmae_Bot2011_COI	ADL-B63	1	BD28961_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	ATGAACAGTCTATCCTCCTTTAG	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28961_Hsan_Wil2021_COI	ADL-B63	1	BD28961_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	CCTGGGCCGGTATAGTAGGT	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28961_Mros_Gib2022_COI	ADL-B63	1	BD28961_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28961_Msal_Bot2012_COI	ADL-B63	1	BD28961_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	GYTAGTTCCRATGATGTTAGCTG	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28961_Mstr_Wil2021_COI	ADL-B63	1	BD28961_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	GTAGTGGTAACTACTCATGCTTTA	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28961_Pvir_Dia2013_COI	ADL-B63	1	BD28961_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	CTTAGTGGCATTAATTCGDAATCC	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28961_Rhar_Sim2018_COI	ADL-B63	1	BD28961_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	CTGCTGCTATTGCTCATG	138.49468
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28963_Aamu_Bax2006_COI	ADL-B61	1	BD28963_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	GCACAACCGGGATCTTTACTTC	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28963_Pcan_Bot2011_IGS	ADL-B61	1	BD28963_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B61	IGS	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	CGTAATCCTCAGTACTGGCTA	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28963_Pper_Dia2013_COI	ADL-B61	1	BD28963_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	CTTAGTGGCATTAATTCGDAATCC	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28963_Sspa_Oph2007_COI	ADL-B61	1	BD28963_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	GAAGGCCTTCCGATGATC	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28963_Upin_Bot2011_COI	ADL-B61	1	BD28963_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	CTTTAATTACAGCGTTTTTATTGTTGT	138.49346
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28964_Cjap_Sim2018_COI	ADL-B60	1	BD28964_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28964_Esin_And2018_Cyb	ADL-B60	1	BD28964_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B60	Cytb	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	ACCCCTCCTCATATCCAACCA	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.651	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28964_Mgig_Bot2012_COI	ADL-B60	1	BD28964_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	TCTTATTCGTTGGAGACTTTATAACCCT	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28964_Mjap_Gib2022_COI	ADL-B60	1	BD28964_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	AATTTAATTGTGACTAGGCATGGTCTG	138.49128
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28965_Cmae_Bot2011_COI	ADL-B59	1	BD28965_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	ATGAACAGTCTATCCTCCTTTAG	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28965_Hsan_Wil2021_COI	ADL-B59	1	BD28965_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	CCTGGGCCGGTATAGTAGGT	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28965_Mros_Gib2022_COI	ADL-B59	1	BD28965_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28965_Msal_Bot2012_COI	ADL-B59	1	BD28965_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	GYTAGTTCCRATGATGTTAGCTG	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28965_Mstr_Wil2021_COI	ADL-B59	1	BD28965_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	GTAGTGGTAACTACTCATGCTTTA	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28965_Pvir_Dia2013_COI	ADL-B59	1	BD28965_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	CTTAGTGGCATTAATTCGDAATCC	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28965_Rhar_Sim2018_COI	ADL-B59	1	BD28965_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	CTGCTGCTATTGCTCATG	138.49026
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28967_Aamu_Bax2006_COI	ADL-B57	1	BD28967_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	GCACAACCGGGATCTTTACTTC	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28967_Pcan_Bot2011_IGS	ADL-B57	1	BD28967_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B57	IGS	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	CGTAATCCTCAGTACTGGCTA	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28967_Pper_Dia2013_COI	ADL-B57	1	BD28967_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	CTTAGTGGCATTAATTCGDAATCC	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28967_Sspa_Oph2007_COI	ADL-B57	1	BD28967_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	GAAGGCCTTCCGATGATC	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28967_Upin_Bot2011_COI	ADL-B57	1	BD28967_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	CTTTAATTACAGCGTTTTTATTGTTGT	138.48711
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28968_Cjap_Sim2018_COI	ADL-B56	1	BD28968_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28968_Esin_And2018_Cyb	ADL-B56	1	BD28968_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B56	Cytb	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	ACCCCTCCTCATATCCAACCA	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.279	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28968_Mgig_Bot2012_COI	ADL-B56	1	BD28968_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	TCTTATTCGTTGGAGACTTTATAACCCT	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28968_Mjap_Gib2022_COI	ADL-B56	1	BD28968_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	AATTTAATTGTGACTAGGCATGGTCTG	138.48611
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28969_Cmae_Bot2011_COI	ADL-B55	1	BD28969_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	ATGAACAGTCTATCCTCCTTTAG	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28969_Hsan_Wil2021_COI	ADL-B55	1	BD28969_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	CCTGGGCCGGTATAGTAGGT	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28969_Mros_Gib2022_COI	ADL-B55	1	BD28969_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28969_Msal_Bot2012_COI	ADL-B55	1	BD28969_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	GYTAGTTCCRATGATGTTAGCTG	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28969_Mstr_Wil2021_COI	ADL-B55	1	BD28969_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	GTAGTGGTAACTACTCATGCTTTA	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28969_Pvir_Dia2013_COI	ADL-B55	1	BD28969_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	CTTAGTGGCATTAATTCGDAATCC	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28969_Rhar_Sim2018_COI	ADL-B55	1	BD28969_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	CTGCTGCTATTGCTCATG	138.48937
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28970_Aamu_Bax2006_COI	ADL-B54	1	BD28970_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	GCACAACCGGGATCTTTACTTC	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28970_Pcan_Bot2011_IGS	ADL-B54	1	BD28970_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B54	IGS	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	CGTAATCCTCAGTACTGGCTA	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28970_Pper_Dia2013_COI	ADL-B54	1	BD28970_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	CTTAGTGGCATTAATTCGDAATCC	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28970_Sspa_Oph2007_COI	ADL-B54	1	BD28970_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	GAAGGCCTTCCGATGATC	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28970_Upin_Bot2011_COI	ADL-B54	1	BD28970_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	CTTTAATTACAGCGTTTTTATTGTTGT	138.48724
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28971_Cjap_Sim2018_COI	ADL-B53	1	BD28971_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28971_Esin_And2018_Cyb	ADL-B53	1	BD28971_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B53	Cytb	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	ACCCCTCCTCATATCCAACCA	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.901	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28971_Mgig_Bot2012_COI	ADL-B53	1	BD28971_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	TCTTATTCGTTGGAGACTTTATAACCCT	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28971_Mjap_Gib2022_COI	ADL-B53	1	BD28971_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	AATTTAATTGTGACTAGGCATGGTCTG	138.48516
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28972_Cmae_Bot2011_COI	ADL-B52	1	BD28972_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	ATGAACAGTCTATCCTCCTTTAG	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28972_Hsan_Wil2021_COI	ADL-B52	1	BD28972_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	CCTGGGCCGGTATAGTAGGT	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28972_Mros_Gib2022_COI	ADL-B52	1	BD28972_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28972_Msal_Bot2012_COI	ADL-B52	1	BD28972_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	GYTAGTTCCRATGATGTTAGCTG	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28972_Mstr_Wil2021_COI	ADL-B52	1	BD28972_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	GTAGTGGTAACTACTCATGCTTTA	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28972_Pvir_Dia2013_COI	ADL-B52	1	BD28972_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	CTTAGTGGCATTAATTCGDAATCC	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28972_Rhar_Sim2018_COI	ADL-B52	1	BD28972_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	CTGCTGCTATTGCTCATG	138.4862
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28974_Aamu_Bax2006_COI	ADL-B50	1	BD28974_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	GCACAACCGGGATCTTTACTTC	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28974_Pcan_Bot2011_IGS	ADL-B50	1	BD28974_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B50	IGS	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	CGTAATCCTCAGTACTGGCTA	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28974_Pper_Dia2013_COI	ADL-B50	1	BD28974_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	CTTAGTGGCATTAATTCGDAATCC	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.136	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28974_Sspa_Oph2007_COI	ADL-B50	1	BD28974_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	GAAGGCCTTCCGATGATC	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28974_Upin_Bot2011_COI	ADL-B50	1	BD28974_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	CTTTAATTACAGCGTTTTTATTGTTGT	138.48525
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28975_Cjap_Sim2018_COI	ADL-B49	1	BD28975_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28975_Esin_And2018_Cyb	ADL-B49	1	BD28975_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B49	Cytb	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	ACCCCTCCTCATATCCAACCA	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28975_Mgig_Bot2012_COI	ADL-B49	1	BD28975_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	TCTTATTCGTTGGAGACTTTATAACCCT	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28975_Mjap_Gib2022_COI	ADL-B49	1	BD28975_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	AATTTAATTGTGACTAGGCATGGTCTG	138.48311
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28976_Cmae_Bot2011_COI	ADL-B48	1	BD28976_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	ATGAACAGTCTATCCTCCTTTAG	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28976_Hsan_Wil2021_COI	ADL-B48	1	BD28976_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	CCTGGGCCGGTATAGTAGGT	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28976_Mros_Gib2022_COI	ADL-B48	1	BD28976_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28976_Msal_Bot2012_COI	ADL-B48	1	BD28976_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	GYTAGTTCCRATGATGTTAGCTG	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28976_Mstr_Wil2021_COI	ADL-B48	1	BD28976_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	GTAGTGGTAACTACTCATGCTTTA	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28976_Pvir_Dia2013_COI	ADL-B48	1	BD28976_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	CTTAGTGGCATTAATTCGDAATCC	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28976_Rhar_Sim2018_COI	ADL-B48	1	BD28976_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	CTGCTGCTATTGCTCATG	138.48421
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28978_Aamu_Bax2006_COI	ADL-B46	1	BD28978_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	GCACAACCGGGATCTTTACTTC	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28978_Pcan_Bot2011_IGS	ADL-B46	1	BD28978_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B46	IGS	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	CGTAATCCTCAGTACTGGCTA	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28978_Pper_Dia2013_COI	ADL-B46	1	BD28978_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	CTTAGTGGCATTAATTCGDAATCC	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28978_Sspa_Oph2007_COI	ADL-B46	1	BD28978_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	GAAGGCCTTCCGATGATC	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28978_Upin_Bot2011_COI	ADL-B46	1	BD28978_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	CTTTAATTACAGCGTTTTTATTGTTGT	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77474	WGS84	BD28978	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28978_Vgib_Wil2025_COI	ADL-B46	1	BD28978_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	CCCCTCTTTACAAGTTCTTTAGGGT	138.48334
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77618	WGS84	BD28979	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28979_Asen_Wil2025_COI	ADL-B45	1	BD28979_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	GTTGTTGTTACAACACATGCTCTTTTG	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28979_Cjap_Sim2018_COI	ADL-B45	1	BD28979_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28979_Esin_And2018_Cyb	ADL-B45	1	BD28979_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B45	Cytb	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	ACCCCTCCTCATATCCAACCA	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.24	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28979_Mgig_Bot2012_COI	ADL-B45	1	BD28979_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	TCTTATTCGTTGGAGACTTTATAACCCT	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28979_Mjap_Gib2022_COI	ADL-B45	1	BD28979_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	AATTTAATTGTGACTAGGCATGGTCTG	138.48234
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28981_Aamu_Bax2006_COI	ADL-B43	1	BD28981_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	GCACAACCGGGATCTTTACTTC	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28981_Pcan_Bot2011_IGS	ADL-B43	1	BD28981_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B43	IGS	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	CGTAATCCTCAGTACTGGCTA	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28981_Pper_Dia2013_COI	ADL-B43	1	BD28981_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	CTTAGTGGCATTAATTCGDAATCC	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28981_Sspa_Oph2007_COI	ADL-B43	1	BD28981_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	GAAGGCCTTCCGATGATC	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28981_Upin_Bot2011_COI	ADL-B43	1	BD28981_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	CTTTAATTACAGCGTTTTTATTGTTGT	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77623	WGS84	BD28981	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28981_Vgib_Wil2025_COI	ADL-B43	1	BD28981_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	CCCCTCTTTACAAGTTCTTTAGGGT	138.4802
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77789	WGS84	BD28982	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28982_Asen_Wil2025_COI	ADL-B42	1	BD28982_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	GTTGTTGTTACAACACATGCTCTTTTG	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28982_Cjap_Sim2018_COI	ADL-B42	1	BD28982_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28982_Esin_And2018_Cyb	ADL-B42	1	BD28982_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B42	Cytb	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	ACCCCTCCTCATATCCAACCA	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28982_Mgig_Bot2012_COI	ADL-B42	1	BD28982_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	TCTTATTCGTTGGAGACTTTATAACCCT	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28982_Mjap_Gib2022_COI	ADL-B42	1	BD28982_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	AATTTAATTGTGACTAGGCATGGTCTG	138.47916
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28983_Cmae_Bot2011_COI	ADL-B41	1	BD28983_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	ATGAACAGTCTATCCTCCTTTAG	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28983_Hsan_Wil2021_COI	ADL-B41	1	BD28983_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	CCTGGGCCGGTATAGTAGGT	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28983_Mros_Gib2022_COI	ADL-B41	1	BD28983_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28983_Msal_Bot2012_COI	ADL-B41	1	BD28983_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	GYTAGTTCCRATGATGTTAGCTG	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28983_Mstr_Wil2021_COI	ADL-B41	1	BD28983_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	GTAGTGGTAACTACTCATGCTTTA	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28983_Pvir_Dia2013_COI	ADL-B41	1	BD28983_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	CTTAGTGGCATTAATTCGDAATCC	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28983_Rhar_Sim2018_COI	ADL-B41	1	BD28983_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	CTGCTGCTATTGCTCATG	138.47823
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28985_Aamu_Bax2006_COI	ADL-B39	1	BD28985_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	GCACAACCGGGATCTTTACTTC	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28985_Pcan_Bot2011_IGS	ADL-B39	1	BD28985_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B39	IGS	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	CGTAATCCTCAGTACTGGCTA	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28985_Pper_Dia2013_COI	ADL-B39	1	BD28985_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	CTTAGTGGCATTAATTCGDAATCC	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28985_Sspa_Oph2007_COI	ADL-B39	1	BD28985_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	GAAGGCCTTCCGATGATC	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28985_Upin_Bot2011_COI	ADL-B39	1	BD28985_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	CTTTAATTACAGCGTTTTTATTGTTGT	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78257	WGS84	BD28985	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28985_Vgib_Wil2025_COI	ADL-B39	1	BD28985_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	CCCCTCTTTACAAGTTCTTTAGGGT	138.47624
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78276	WGS84	BD28986	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28986_Asen_Wil2025_COI	ADL-B38	1	BD28986_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	GTTGTTGTTACAACACATGCTCTTTTG	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28986_Cjap_Sim2018_COI	ADL-B38	1	BD28986_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28986_Esin_And2018_Cyb	ADL-B38	1	BD28986_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B38	Cytb	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	ACCCCTCCTCATATCCAACCA	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28986_Mgig_Bot2012_COI	ADL-B38	1	BD28986_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	TCTTATTCGTTGGAGACTTTATAACCCT	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28986_Mjap_Gib2022_COI	ADL-B38	1	BD28986_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	AATTTAATTGTGACTAGGCATGGTCTG	138.47417
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28987_Cmae_Bot2011_COI	ADL-B37	1	BD28987_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	ATGAACAGTCTATCCTCCTTTAG	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28987_Hsan_Wil2021_COI	ADL-B37	1	BD28987_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	CCTGGGCCGGTATAGTAGGT	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28987_Mros_Gib2022_COI	ADL-B37	1	BD28987_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28987_Msal_Bot2012_COI	ADL-B37	1	BD28987_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	GYTAGTTCCRATGATGTTAGCTG	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28987_Mstr_Wil2021_COI	ADL-B37	1	BD28987_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	GTAGTGGTAACTACTCATGCTTTA	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28987_Pvir_Dia2013_COI	ADL-B37	1	BD28987_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	CTTAGTGGCATTAATTCGDAATCC	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28987_Rhar_Sim2018_COI	ADL-B37	1	BD28987_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	CTGCTGCTATTGCTCATG	138.47512
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28989_Aamu_Bax2006_COI	PLO-B40	1	BD28989_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	GCACAACCGGGATCTTTACTTC	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28989_Pcan_Bot2011_IGS	PLO-B40	1	BD28989_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B40	IGS	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	CGTAATCCTCAGTACTGGCTA	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28989_Pper_Dia2013_COI	PLO-B40	1	BD28989_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	CTTAGTGGCATTAATTCGDAATCC	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.125	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28989_Sspa_Oph2007_COI	PLO-B40	1	BD28989_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	GAAGGCCTTCCGATGATC	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28989_Upin_Bot2011_COI	PLO-B40	1	BD28989_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	CTTTAATTACAGCGTTTTTATTGTTGT	135.86778
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28990_Cmae_Bot2011_COI	PLO-B39	1	BD28990_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	ATGAACAGTCTATCCTCCTTTAG	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28990_Hsan_Wil2021_COI	PLO-B39	1	BD28990_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	CCTGGGCCGGTATAGTAGGT	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28990_Mros_Gib2022_COI	PLO-B39	1	BD28990_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28990_Msal_Bot2012_COI	PLO-B39	1	BD28990_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	GYTAGTTCCRATGATGTTAGCTG	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28990_Mstr_Wil2021_COI	PLO-B39	1	BD28990_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	GTAGTGGTAACTACTCATGCTTTA	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28990_Pvir_Dia2013_COI	PLO-B39	1	BD28990_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	CTTAGTGGCATTAATTCGDAATCC	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28990_Rhar_Sim2018_COI	PLO-B39	1	BD28990_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	CTGCTGCTATTGCTCATG	135.86996
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28992_Aamu_Bax2006_COI	PLO-B37	1	BD28992_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	GCACAACCGGGATCTTTACTTC	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28992_Pcan_Bot2011_IGS	PLO-B37	1	BD28992_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B37	IGS	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	CGTAATCCTCAGTACTGGCTA	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28992_Pper_Dia2013_COI	PLO-B37	1	BD28992_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	CTTAGTGGCATTAATTCGDAATCC	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28992_Sspa_Oph2007_COI	PLO-B37	1	BD28992_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	GAAGGCCTTCCGATGATC	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28992_Upin_Bot2011_COI	PLO-B37	1	BD28992_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	CTTTAATTACAGCGTTTTTATTGTTGT	135.87379
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28993_Cjap_Sim2018_COI	PLO-B36	1	BD28993_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28993_Esin_And2018_Cyb	PLO-B36	1	BD28993_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B36	Cytb	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	ACCCCTCCTCATATCCAACCA	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28993_Mgig_Bot2012_COI	PLO-B36	1	BD28993_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	TCTTATTCGTTGGAGACTTTATAACCCT	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28993_Mjap_Gib2022_COI	PLO-B36	1	BD28993_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	AATTTAATTGTGACTAGGCATGGTCTG	135.87749
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28994_Cmae_Bot2011_COI	THE-B35	1	BD28994_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	ATGAACAGTCTATCCTCCTTTAG	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28994_Hsan_Wil2021_COI	THE-B35	1	BD28994_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	CCTGGGCCGGTATAGTAGGT	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28994_Mros_Gib2022_COI	THE-B35	1	BD28994_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28994_Msal_Bot2012_COI	THE-B35	1	BD28994_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	GYTAGTTCCRATGATGTTAGCTG	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28994_Mstr_Wil2021_COI	THE-B35	1	BD28994_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	GTAGTGGTAACTACTCATGCTTTA	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28994_Pvir_Dia2013_COI	THE-B35	1	BD28994_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	CTTAGTGGCATTAATTCGDAATCC	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28994_Rhar_Sim2018_COI	THE-B35	1	BD28994_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	CTGCTGCTATTGCTCATG	133.63829
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28996_Aamu_Bax2006_COI	THE-B33	1	BD28996_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	GCACAACCGGGATCTTTACTTC	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28996_Pcan_Bot2011_IGS	THE-B33	1	BD28996_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B33	IGS	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	CGTAATCCTCAGTACTGGCTA	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28996_Pper_Dia2013_COI	THE-B33	1	BD28996_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	CTTAGTGGCATTAATTCGDAATCC	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28996_Sspa_Oph2007_COI	THE-B33	1	BD28996_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	GAAGGCCTTCCGATGATC	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28996_Upin_Bot2011_COI	THE-B33	1	BD28996_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	CTTTAATTACAGCGTTTTTATTGTTGT	133.64047
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28997_Cjap_Sim2018_COI	THE-B32	1	BD28997_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28997_Esin_And2018_Cyb	THE-B32	1	BD28997_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B32	Cytb	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	ACCCCTCCTCATATCCAACCA	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.969	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28997_Mgig_Bot2012_COI	THE-B32	1	BD28997_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	TCTTATTCGTTGGAGACTTTATAACCCT	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28997_Mjap_Gib2022_COI	THE-B32	1	BD28997_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	AATTTAATTGTGACTAGGCATGGTCTG	133.63493
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28998_Cmae_Bot2011_COI	THE-B31	1	BD28998_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	ATGAACAGTCTATCCTCCTTTAG	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28998_Hsan_Wil2021_COI	THE-B31	1	BD28998_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	CCTGGGCCGGTATAGTAGGT	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28998_Mros_Gib2022_COI	THE-B31	1	BD28998_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28998_Msal_Bot2012_COI	THE-B31	1	BD28998_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	GYTAGTTCCRATGATGTTAGCTG	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28998_Mstr_Wil2021_COI	THE-B31	1	BD28998_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	GTAGTGGTAACTACTCATGCTTTA	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28998_Pvir_Dia2013_COI	THE-B31	1	BD28998_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	CTTAGTGGCATTAATTCGDAATCC	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28998_Rhar_Sim2018_COI	THE-B31	1	BD28998_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	CTGCTGCTATTGCTCATG	133.63652
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29000_Cjap_Sim2018_COI	THE-B29	1	BD29000_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29000_Esin_And2018_Cyb	THE-B29	1	BD29000_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B29	Cytb	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	ACCCCTCCTCATATCCAACCA	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.605	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29000_Mgig_Bot2012_COI	THE-B29	1	BD29000_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	TCTTATTCGTTGGAGACTTTATAACCCT	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29000_Mjap_Gib2022_COI	THE-B29	1	BD29000_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	AATTTAATTGTGACTAGGCATGGTCTG	133.6387
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29001_Cmae_Bot2011_COI	THE-B28	1	BD29001_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	ATGAACAGTCTATCCTCCTTTAG	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29001_Hsan_Wil2021_COI	THE-B28	1	BD29001_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	CCTGGGCCGGTATAGTAGGT	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29001_Mros_Gib2022_COI	THE-B28	1	BD29001_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29001_Msal_Bot2012_COI	THE-B28	1	BD29001_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	GYTAGTTCCRATGATGTTAGCTG	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29001_Mstr_Wil2021_COI	THE-B28	1	BD29001_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	GTAGTGGTAACTACTCATGCTTTA	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29001_Pvir_Dia2013_COI	THE-B28	1	BD29001_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	CTTAGTGGCATTAATTCGDAATCC	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29001_Rhar_Sim2018_COI	THE-B28	1	BD29001_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	CTGCTGCTATTGCTCATG	133.63921
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29003_Aamu_Bax2006_COI	THE-B26	1	BD29003_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	GCACAACCGGGATCTTTACTTC	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29003_Pcan_Bot2011_IGS	THE-B26	1	BD29003_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B26	IGS	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	CGTAATCCTCAGTACTGGCTA	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29003_Pper_Dia2013_COI	THE-B26	1	BD29003_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	CTTAGTGGCATTAATTCGDAATCC	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29003_Sspa_Oph2007_COI	THE-B26	1	BD29003_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	GAAGGCCTTCCGATGATC	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29003_Upin_Bot2011_COI	THE-B26	1	BD29003_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	CTTTAATTACAGCGTTTTTATTGTTGT	133.63596
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29004_Cjap_Sim2018_COI	THE-B25	1	BD29004_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29004_Esin_And2018_Cyb	THE-B25	1	BD29004_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B25	Cytb	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	ACCCCTCCTCATATCCAACCA	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.087	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29004_Mgig_Bot2012_COI	THE-B25	1	BD29004_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	TCTTATTCGTTGGAGACTTTATAACCCT	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29004_Mjap_Gib2022_COI	THE-B25	1	BD29004_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	AATTTAATTGTGACTAGGCATGGTCTG	133.63725
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29005_Cmae_Bot2011_COI	THE-B24	1	BD29005_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	ATGAACAGTCTATCCTCCTTTAG	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29005_Hsan_Wil2021_COI	THE-B24	1	BD29005_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	CCTGGGCCGGTATAGTAGGT	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29005_Mros_Gib2022_COI	THE-B24	1	BD29005_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29005_Msal_Bot2012_COI	THE-B24	1	BD29005_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	GYTAGTTCCRATGATGTTAGCTG	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29005_Mstr_Wil2021_COI	THE-B24	1	BD29005_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	GTAGTGGTAACTACTCATGCTTTA	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29005_Pvir_Dia2013_COI	THE-B24	1	BD29005_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	CTTAGTGGCATTAATTCGDAATCC	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29005_Rhar_Sim2018_COI	THE-B24	1	BD29005_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	CTGCTGCTATTGCTCATG	133.63886
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29007_Aamu_Bax2006_COI	THE-B22	1	BD29007_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	GCACAACCGGGATCTTTACTTC	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29007_Pcan_Bot2011_IGS	THE-B22	1	BD29007_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B22	IGS	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	CGTAATCCTCAGTACTGGCTA	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29007_Pper_Dia2013_COI	THE-B22	1	BD29007_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	CTTAGTGGCATTAATTCGDAATCC	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29007_Sspa_Oph2007_COI	THE-B22	1	BD29007_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	GAAGGCCTTCCGATGATC	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29007_Upin_Bot2011_COI	THE-B22	1	BD29007_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	CTTTAATTACAGCGTTTTTATTGTTGT	133.64094
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29008_Cjap_Sim2018_COI	THE-B21	1	BD29008_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29008_Esin_And2018_Cyb	THE-B21	1	BD29008_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B21	Cytb	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	ACCCCTCCTCATATCCAACCA	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.549	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29008_Mgig_Bot2012_COI	THE-B21	1	BD29008_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	TCTTATTCGTTGGAGACTTTATAACCCT	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29008_Mjap_Gib2022_COI	THE-B21	1	BD29008_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	AATTTAATTGTGACTAGGCATGGTCTG	133.63514
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29009_Cmae_Bot2011_COI	THE-B20	1	BD29009_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	ATGAACAGTCTATCCTCCTTTAG	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29009_Hsan_Wil2021_COI	THE-B20	1	BD29009_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	CCTGGGCCGGTATAGTAGGT	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29009_Mros_Gib2022_COI	THE-B20	1	BD29009_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29009_Msal_Bot2012_COI	THE-B20	1	BD29009_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	GYTAGTTCCRATGATGTTAGCTG	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29009_Mstr_Wil2021_COI	THE-B20	1	BD29009_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	GTAGTGGTAACTACTCATGCTTTA	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29009_Pvir_Dia2013_COI	THE-B20	1	BD29009_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	CTTAGTGGCATTAATTCGDAATCC	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29009_Rhar_Sim2018_COI	THE-B20	1	BD29009_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	CTGCTGCTATTGCTCATG	133.63656
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29010_Aamu_Bax2006_COI	THE-B19	1	BD29010_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	GCACAACCGGGATCTTTACTTC	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29010_Pcan_Bot2011_IGS	THE-B19	1	BD29010_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B19	IGS	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	CGTAATCCTCAGTACTGGCTA	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29010_Pper_Dia2013_COI	THE-B19	1	BD29010_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	CTTAGTGGCATTAATTCGDAATCC	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29010_Sspa_Oph2007_COI	THE-B19	1	BD29010_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	GAAGGCCTTCCGATGATC	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29010_Upin_Bot2011_COI	THE-B19	1	BD29010_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	CTTTAATTACAGCGTTTTTATTGTTGT	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.14915	WGS84	BD29010	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29010_Vgib_Wil2025_COI	THE-B19	1	BD29010_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	CCCCTCTTTACAAGTTCTTTAGGGT	133.63803
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15349	WGS84	BD29011	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29011_Asen_Wil2025_COI	THE-B18	1	BD29011_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	GTTGTTGTTACAACACATGCTCTTTTG	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29011_Cjap_Sim2018_COI	THE-B18	1	BD29011_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29011_Esin_And2018_Cyb	THE-B18	1	BD29011_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B18	Cytb	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	ACCCCTCCTCATATCCAACCA	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29011_Mgig_Bot2012_COI	THE-B18	1	BD29011_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	TCTTATTCGTTGGAGACTTTATAACCCT	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29011_Mjap_Gib2022_COI	THE-B18	1	BD29011_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	AATTTAATTGTGACTAGGCATGGTCTG	133.63787
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29012_Cmae_Bot2011_COI	THE-B17	1	BD29012_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	ATGAACAGTCTATCCTCCTTTAG	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29012_Hsan_Wil2021_COI	THE-B17	1	BD29012_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	CCTGGGCCGGTATAGTAGGT	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29012_Mros_Gib2022_COI	THE-B17	1	BD29012_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29012_Msal_Bot2012_COI	THE-B17	1	BD29012_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	GYTAGTTCCRATGATGTTAGCTG	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29012_Mstr_Wil2021_COI	THE-B17	1	BD29012_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	GTAGTGGTAACTACTCATGCTTTA	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29012_Pvir_Dia2013_COI	THE-B17	1	BD29012_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	CTTAGTGGCATTAATTCGDAATCC	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29012_Rhar_Sim2018_COI	THE-B17	1	BD29012_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	CTGCTGCTATTGCTCATG	133.63895
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29014_Aamu_Bax2006_COI	THE-B15	1	BD29014_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	GCACAACCGGGATCTTTACTTC	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29014_Pcan_Bot2011_IGS	THE-B15	1	BD29014_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B15	IGS	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	CGTAATCCTCAGTACTGGCTA	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29014_Pper_Dia2013_COI	THE-B15	1	BD29014_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	CTTAGTGGCATTAATTCGDAATCC	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29014_Sspa_Oph2007_COI	THE-B15	1	BD29014_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	GAAGGCCTTCCGATGATC	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29014_Upin_Bot2011_COI	THE-B15	1	BD29014_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	CTTTAATTACAGCGTTTTTATTGTTGT	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15215	WGS84	BD29014	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29014_Vgib_Wil2025_COI	THE-B15	1	BD29014_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	CCCCTCTTTACAAGTTCTTTAGGGT	133.63988
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15222	WGS84	BD29015	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29015_Asen_Wil2025_COI	THE-B14	1	BD29015_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	GTTGTTGTTACAACACATGCTCTTTTG	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29015_Cjap_Sim2018_COI	THE-B14	1	BD29015_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29015_Esin_And2018_Cyb	THE-B14	1	BD29015_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B14	Cytb	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	ACCCCTCCTCATATCCAACCA	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.301	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29015_Mgig_Bot2012_COI	THE-B14	1	BD29015_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	TCTTATTCGTTGGAGACTTTATAACCCT	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29015_Mjap_Gib2022_COI	THE-B14	1	BD29015_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	AATTTAATTGTGACTAGGCATGGTCTG	133.63854
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29016_Cmae_Bot2011_COI	THE-B13	1	BD29016_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	ATGAACAGTCTATCCTCCTTTAG	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29016_Hsan_Wil2021_COI	THE-B13	1	BD29016_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	CCTGGGCCGGTATAGTAGGT	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29016_Mros_Gib2022_COI	THE-B13	1	BD29016_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29016_Msal_Bot2012_COI	THE-B13	1	BD29016_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	GYTAGTTCCRATGATGTTAGCTG	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29016_Mstr_Wil2021_COI	THE-B13	1	BD29016_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	GTAGTGGTAACTACTCATGCTTTA	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29016_Pvir_Dia2013_COI	THE-B13	1	BD29016_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	CTTAGTGGCATTAATTCGDAATCC	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29016_Rhar_Sim2018_COI	THE-B13	1	BD29016_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	CTGCTGCTATTGCTCATG	133.63703
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29018_Aamu_Bax2006_COI	THE-B11	1	BD29018_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	GCACAACCGGGATCTTTACTTC	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29018_Pcan_Bot2011_IGS	THE-B11	1	BD29018_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B11	IGS	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	CGTAATCCTCAGTACTGGCTA	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29018_Pper_Dia2013_COI	THE-B11	1	BD29018_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	CTTAGTGGCATTAATTCGDAATCC	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29018_Sspa_Oph2007_COI	THE-B11	1	BD29018_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	GAAGGCCTTCCGATGATC	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29018_Upin_Bot2011_COI	THE-B11	1	BD29018_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	CTTTAATTACAGCGTTTTTATTGTTGT	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.151	WGS84	BD29018	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29018_Vgib_Wil2025_COI	THE-B11	1	BD29018_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	CCCCTCTTTACAAGTTCTTTAGGGT	133.6357
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15113	WGS84	BD29019	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29019_Asen_Wil2025_COI	THE-B10	1	BD29019_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	GTTGTTGTTACAACACATGCTCTTTTG	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29019_Cjap_Sim2018_COI	THE-B10	1	BD29019_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29019_Esin_And2018_Cyb	THE-B10	1	BD29019_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B10	Cytb	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	ACCCCTCCTCATATCCAACCA	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29019_Mgig_Bot2012_COI	THE-B10	1	BD29019_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	TCTTATTCGTTGGAGACTTTATAACCCT	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29019_Mjap_Gib2022_COI	THE-B10	1	BD29019_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	AATTTAATTGTGACTAGGCATGGTCTG	133.63644
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29021_Aamu_Bax2006_COI	THE-B08	1	BD29021_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	GCACAACCGGGATCTTTACTTC	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29021_Pcan_Bot2011_IGS	THE-B08	1	BD29021_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B08	IGS	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	CGTAATCCTCAGTACTGGCTA	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29021_Pper_Dia2013_COI	THE-B08	1	BD29021_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	CTTAGTGGCATTAATTCGDAATCC	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29021_Sspa_Oph2007_COI	THE-B08	1	BD29021_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	GAAGGCCTTCCGATGATC	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29021_Upin_Bot2011_COI	THE-B08	1	BD29021_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	CTTTAATTACAGCGTTTTTATTGTTGT	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15129	WGS84	BD29021	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29021_Vgib_Wil2025_COI	THE-B08	1	BD29021_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	CCCCTCTTTACAAGTTCTTTAGGGT	133.63934
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15125	WGS84	BD29022	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29022_Asen_Wil2025_COI	THE-B07	1	BD29022_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	GTTGTTGTTACAACACATGCTCTTTTG	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29022_Cjap_Sim2018_COI	THE-B07	1	BD29022_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29022_Esin_And2018_Cyb	THE-B07	1	BD29022_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B07	Cytb	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	ACCCCTCCTCATATCCAACCA	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.93	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29022_Mgig_Bot2012_COI	THE-B07	1	BD29022_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	TCTTATTCGTTGGAGACTTTATAACCCT	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29022_Mjap_Gib2022_COI	THE-B07	1	BD29022_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	AATTTAATTGTGACTAGGCATGGTCTG	133.64093
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29023_Cmae_Bot2011_COI	THE-B06	1	BD29023_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	ATGAACAGTCTATCCTCCTTTAG	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29023_Hsan_Wil2021_COI	THE-B06	1	BD29023_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	CCTGGGCCGGTATAGTAGGT	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29023_Mros_Gib2022_COI	THE-B06	1	BD29023_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29023_Msal_Bot2012_COI	THE-B06	1	BD29023_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	GYTAGTTCCRATGATGTTAGCTG	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29023_Mstr_Wil2021_COI	THE-B06	1	BD29023_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	GTAGTGGTAACTACTCATGCTTTA	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29023_Pvir_Dia2013_COI	THE-B06	1	BD29023_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	CTTAGTGGCATTAATTCGDAATCC	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29023_Rhar_Sim2018_COI	THE-B06	1	BD29023_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	CTGCTGCTATTGCTCATG	133.64194
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29025_Aamu_Bax2006_COI	THE-B04	1	BD29025_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	GCACAACCGGGATCTTTACTTC	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29025_Pcan_Bot2011_IGS	THE-B04	1	BD29025_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B04	IGS	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	CGTAATCCTCAGTACTGGCTA	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29025_Pper_Dia2013_COI	THE-B04	1	BD29025_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	CTTAGTGGCATTAATTCGDAATCC	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29025_Sspa_Oph2007_COI	THE-B04	1	BD29025_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	GAAGGCCTTCCGATGATC	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29025_Upin_Bot2011_COI	THE-B04	1	BD29025_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	CTTTAATTACAGCGTTTTTATTGTTGT	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15014	WGS84	BD29025	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29025_Vgib_Wil2025_COI	THE-B04	1	BD29025_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	CCCCTCTTTACAAGTTCTTTAGGGT	133.63966
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29026_Cjap_Sim2018_COI	THE-B03	1	BD29026_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29026_Esin_And2018_Cyb	THE-B03	1	BD29026_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B03	Cytb	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	ACCCCTCCTCATATCCAACCA	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.617	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29026_Mgig_Bot2012_COI	THE-B03	1	BD29026_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	TCTTATTCGTTGGAGACTTTATAACCCT	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29026_Mjap_Gib2022_COI	THE-B03	1	BD29026_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	AATTTAATTGTGACTAGGCATGGTCTG	133.63801
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29027_Cmae_Bot2011_COI	THE-B02	1	BD29027_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	ATGAACAGTCTATCCTCCTTTAG	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29027_Hsan_Wil2021_COI	THE-B02	1	BD29027_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	CCTGGGCCGGTATAGTAGGT	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29027_Mros_Gib2022_COI	THE-B02	1	BD29027_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29027_Msal_Bot2012_COI	THE-B02	1	BD29027_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	GYTAGTTCCRATGATGTTAGCTG	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29027_Mstr_Wil2021_COI	THE-B02	1	BD29027_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	GTAGTGGTAACTACTCATGCTTTA	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29027_Pvir_Dia2013_COI	THE-B02	1	BD29027_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	CTTAGTGGCATTAATTCGDAATCC	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29027_Rhar_Sim2018_COI	THE-B02	1	BD29027_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	CTGCTGCTATTGCTCATG	133.63706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29029_Aamu_Bax2006_COI	PGI-B35	1	BD29029_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	GCACAACCGGGATCTTTACTTC	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29029_Pcan_Bot2011_IGS	PGI-B35	1	BD29029_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B35	IGS	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	CGTAATCCTCAGTACTGGCTA	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29029_Pper_Dia2013_COI	PGI-B35	1	BD29029_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	CTTAGTGGCATTAATTCGDAATCC	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29029_Sspa_Oph2007_COI	PGI-B35	1	BD29029_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	GAAGGCCTTCCGATGATC	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29029_Upin_Bot2011_COI	PGI-B35	1	BD29029_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	CTTTAATTACAGCGTTTTTATTGTTGT	137.7706
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29030_Cmae_Bot2011_COI	PGI-B34	1	BD29030_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	ATGAACAGTCTATCCTCCTTTAG	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29030_Hsan_Wil2021_COI	PGI-B34	1	BD29030_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	CCTGGGCCGGTATAGTAGGT	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29030_Mros_Gib2022_COI	PGI-B34	1	BD29030_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29030_Msal_Bot2012_COI	PGI-B34	1	BD29030_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	GYTAGTTCCRATGATGTTAGCTG	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29030_Mstr_Wil2021_COI	PGI-B34	1	BD29030_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	GTAGTGGTAACTACTCATGCTTTA	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29030_Pvir_Dia2013_COI	PGI-B34	1	BD29030_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	CTTAGTGGCATTAATTCGDAATCC	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29030_Rhar_Sim2018_COI	PGI-B34	1	BD29030_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	CTGCTGCTATTGCTCATG	137.76884
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29032_Aamu_Bax2006_COI	PGI-B32	1	BD29032_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	GCACAACCGGGATCTTTACTTC	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29032_Pcan_Bot2011_IGS	PGI-B32	1	BD29032_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B32	IGS	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	CGTAATCCTCAGTACTGGCTA	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29032_Pper_Dia2013_COI	PGI-B32	1	BD29032_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	CTTAGTGGCATTAATTCGDAATCC	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29032_Sspa_Oph2007_COI	PGI-B32	1	BD29032_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	GAAGGCCTTCCGATGATC	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29032_Upin_Bot2011_COI	PGI-B32	1	BD29032_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	CTTTAATTACAGCGTTTTTATTGTTGT	137.76542
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29033_Cjap_Sim2018_COI	PGI-B31	1	BD29033_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29033_Esin_And2018_Cyb	PGI-B31	1	BD29033_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B31	Cytb	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	ACCCCTCCTCATATCCAACCA	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29033_Mgig_Bot2012_COI	PGI-B31	1	BD29033_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	TCTTATTCGTTGGAGACTTTATAACCCT	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29033_Mjap_Gib2022_COI	PGI-B31	1	BD29033_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	AATTTAATTGTGACTAGGCATGGTCTG	137.76381
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29034_Cmae_Bot2011_COI	PGI-B30	1	BD29034_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	ATGAACAGTCTATCCTCCTTTAG	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29034_Hsan_Wil2021_COI	PGI-B30	1	BD29034_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	CCTGGGCCGGTATAGTAGGT	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29034_Mros_Gib2022_COI	PGI-B30	1	BD29034_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29034_Msal_Bot2012_COI	PGI-B30	1	BD29034_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	GYTAGTTCCRATGATGTTAGCTG	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29034_Mstr_Wil2021_COI	PGI-B30	1	BD29034_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	GTAGTGGTAACTACTCATGCTTTA	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29034_Pvir_Dia2013_COI	PGI-B30	1	BD29034_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	CTTAGTGGCATTAATTCGDAATCC	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29034_Rhar_Sim2018_COI	PGI-B30	1	BD29034_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	CTGCTGCTATTGCTCATG	137.77147
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29036_Aamu_Bax2006_COI	PGI-B28	1	BD29036_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	GCACAACCGGGATCTTTACTTC	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29036_Pcan_Bot2011_IGS	PGI-B28	1	BD29036_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B28	IGS	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	CGTAATCCTCAGTACTGGCTA	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29036_Pper_Dia2013_COI	PGI-B28	1	BD29036_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	CTTAGTGGCATTAATTCGDAATCC	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29036_Sspa_Oph2007_COI	PGI-B28	1	BD29036_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	GAAGGCCTTCCGATGATC	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29036_Upin_Bot2011_COI	PGI-B28	1	BD29036_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	CTTTAATTACAGCGTTTTTATTGTTGT	137.76811
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29037_Cjap_Sim2018_COI	PGI-B27	1	BD29037_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29037_Esin_And2018_Cyb	PGI-B27	1	BD29037_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B27	Cytb	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	ACCCCTCCTCATATCCAACCA	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29037_Mgig_Bot2012_COI	PGI-B27	1	BD29037_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	TCTTATTCGTTGGAGACTTTATAACCCT	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29037_Mjap_Gib2022_COI	PGI-B27	1	BD29037_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	AATTTAATTGTGACTAGGCATGGTCTG	137.7663
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29038_Cmae_Bot2011_COI	PGI-B26	1	BD29038_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	ATGAACAGTCTATCCTCCTTTAG	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29038_Hsan_Wil2021_COI	PGI-B26	1	BD29038_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	CCTGGGCCGGTATAGTAGGT	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29038_Mros_Gib2022_COI	PGI-B26	1	BD29038_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29038_Msal_Bot2012_COI	PGI-B26	1	BD29038_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	GYTAGTTCCRATGATGTTAGCTG	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29038_Mstr_Wil2021_COI	PGI-B26	1	BD29038_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	GTAGTGGTAACTACTCATGCTTTA	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29038_Pvir_Dia2013_COI	PGI-B26	1	BD29038_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	CTTAGTGGCATTAATTCGDAATCC	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29038_Rhar_Sim2018_COI	PGI-B26	1	BD29038_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	CTGCTGCTATTGCTCATG	137.7646
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29040_Cjap_Sim2018_COI	PGI-B24	1	BD29040_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29040_Esin_And2018_Cyb	PGI-B24	1	BD29040_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B24	Cytb	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	ACCCCTCCTCATATCCAACCA	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29040_Mgig_Bot2012_COI	PGI-B24	1	BD29040_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	TCTTATTCGTTGGAGACTTTATAACCCT	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29040_Mjap_Gib2022_COI	PGI-B24	1	BD29040_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	AATTTAATTGTGACTAGGCATGGTCTG	137.77071
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29041_Cmae_Bot2011_COI	PGI-B23	1	BD29041_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	ATGAACAGTCTATCCTCCTTTAG	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29041_Hsan_Wil2021_COI	PGI-B23	1	BD29041_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	CCTGGGCCGGTATAGTAGGT	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29041_Mros_Gib2022_COI	PGI-B23	1	BD29041_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29041_Msal_Bot2012_COI	PGI-B23	1	BD29041_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	GYTAGTTCCRATGATGTTAGCTG	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29041_Mstr_Wil2021_COI	PGI-B23	1	BD29041_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	GTAGTGGTAACTACTCATGCTTTA	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29041_Pvir_Dia2013_COI	PGI-B23	1	BD29041_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	CTTAGTGGCATTAATTCGDAATCC	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29041_Rhar_Sim2018_COI	PGI-B23	1	BD29041_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	CTGCTGCTATTGCTCATG	137.76897
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29043_Aamu_Bax2006_COI	PGI-B21	1	BD29043_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	GCACAACCGGGATCTTTACTTC	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29043_Pcan_Bot2011_IGS	PGI-B21	1	BD29043_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B21	IGS	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	CGTAATCCTCAGTACTGGCTA	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29043_Pper_Dia2013_COI	PGI-B21	1	BD29043_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	CTTAGTGGCATTAATTCGDAATCC	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29043_Sspa_Oph2007_COI	PGI-B21	1	BD29043_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	GAAGGCCTTCCGATGATC	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29043_Upin_Bot2011_COI	PGI-B21	1	BD29043_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	CTTTAATTACAGCGTTTTTATTGTTGT	137.76543
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29044_Cjap_Sim2018_COI	PGI-B20	1	BD29044_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29044_Esin_And2018_Cyb	PGI-B20	1	BD29044_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B20	Cytb	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	ACCCCTCCTCATATCCAACCA	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29044_Mgig_Bot2012_COI	PGI-B20	1	BD29044_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	TCTTATTCGTTGGAGACTTTATAACCCT	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29044_Mjap_Gib2022_COI	PGI-B20	1	BD29044_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	AATTTAATTGTGACTAGGCATGGTCTG	137.76397
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29045_Cmae_Bot2011_COI	PGI-B19	1	BD29045_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	ATGAACAGTCTATCCTCCTTTAG	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29045_Hsan_Wil2021_COI	PGI-B19	1	BD29045_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	CCTGGGCCGGTATAGTAGGT	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29045_Mros_Gib2022_COI	PGI-B19	1	BD29045_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29045_Msal_Bot2012_COI	PGI-B19	1	BD29045_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	GYTAGTTCCRATGATGTTAGCTG	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29045_Mstr_Wil2021_COI	PGI-B19	1	BD29045_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	GTAGTGGTAACTACTCATGCTTTA	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29045_Pvir_Dia2013_COI	PGI-B19	1	BD29045_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	CTTAGTGGCATTAATTCGDAATCC	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29045_Rhar_Sim2018_COI	PGI-B19	1	BD29045_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	CTGCTGCTATTGCTCATG	137.77334
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29047_Aamu_Bax2006_COI	PGI-B17	1	BD29047_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	GCACAACCGGGATCTTTACTTC	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29047_Pcan_Bot2011_IGS	PGI-B17	1	BD29047_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B17	IGS	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	CGTAATCCTCAGTACTGGCTA	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29047_Pper_Dia2013_COI	PGI-B17	1	BD29047_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	CTTAGTGGCATTAATTCGDAATCC	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29047_Sspa_Oph2007_COI	PGI-B17	1	BD29047_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	GAAGGCCTTCCGATGATC	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29047_Upin_Bot2011_COI	PGI-B17	1	BD29047_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	CTTTAATTACAGCGTTTTTATTGTTGT	137.76988
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29048_Cjap_Sim2018_COI	PGI-B16	1	BD29048_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29048_Esin_And2018_Cyb	PGI-B16	1	BD29048_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B16	Cytb	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	ACCCCTCCTCATATCCAACCA	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29048_Mgig_Bot2012_COI	PGI-B16	1	BD29048_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	TCTTATTCGTTGGAGACTTTATAACCCT	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29048_Mjap_Gib2022_COI	PGI-B16	1	BD29048_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	AATTTAATTGTGACTAGGCATGGTCTG	137.77252
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29049_Cmae_Bot2011_COI	PGI-B15	1	BD29049_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	ATGAACAGTCTATCCTCCTTTAG	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29049_Hsan_Wil2021_COI	PGI-B15	1	BD29049_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	CCTGGGCCGGTATAGTAGGT	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29049_Mros_Gib2022_COI	PGI-B15	1	BD29049_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29049_Msal_Bot2012_COI	PGI-B15	1	BD29049_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	GYTAGTTCCRATGATGTTAGCTG	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29049_Mstr_Wil2021_COI	PGI-B15	1	BD29049_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	GTAGTGGTAACTACTCATGCTTTA	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29049_Pvir_Dia2013_COI	PGI-B15	1	BD29049_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	CTTAGTGGCATTAATTCGDAATCC	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29049_Rhar_Sim2018_COI	PGI-B15	1	BD29049_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	CTGCTGCTATTGCTCATG	137.77077
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29050_Aamu_Bax2006_COI	PGI-B14	1	BD29050_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	GCACAACCGGGATCTTTACTTC	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29050_Pcan_Bot2011_IGS	PGI-B14	1	BD29050_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B14	IGS	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	CGTAATCCTCAGTACTGGCTA	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29050_Pper_Dia2013_COI	PGI-B14	1	BD29050_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	CTTAGTGGCATTAATTCGDAATCC	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29050_Sspa_Oph2007_COI	PGI-B14	1	BD29050_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	GAAGGCCTTCCGATGATC	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29050_Upin_Bot2011_COI	PGI-B14	1	BD29050_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	CTTTAATTACAGCGTTTTTATTGTTGT	137.76906
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29051_Cjap_Sim2018_COI	PGI-B13	1	BD29051_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29051_Esin_And2018_Cyb	PGI-B13	1	BD29051_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B13	Cytb	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	ACCCCTCCTCATATCCAACCA	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29051_Mgig_Bot2012_COI	PGI-B13	1	BD29051_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	TCTTATTCGTTGGAGACTTTATAACCCT	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29051_Mjap_Gib2022_COI	PGI-B13	1	BD29051_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	AATTTAATTGTGACTAGGCATGGTCTG	137.7673
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29052_Cmae_Bot2011_COI	PGI-B12	1	BD29052_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	ATGAACAGTCTATCCTCCTTTAG	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29052_Hsan_Wil2021_COI	PGI-B12	1	BD29052_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	CCTGGGCCGGTATAGTAGGT	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29052_Mros_Gib2022_COI	PGI-B12	1	BD29052_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29052_Msal_Bot2012_COI	PGI-B12	1	BD29052_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	GYTAGTTCCRATGATGTTAGCTG	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29052_Mstr_Wil2021_COI	PGI-B12	1	BD29052_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	GTAGTGGTAACTACTCATGCTTTA	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29052_Pvir_Dia2013_COI	PGI-B12	1	BD29052_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	CTTAGTGGCATTAATTCGDAATCC	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29052_Rhar_Sim2018_COI	PGI-B12	1	BD29052_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	CTGCTGCTATTGCTCATG	137.76561
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29054_Aamu_Bax2006_COI	PGI-B10	1	BD29054_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	GCACAACCGGGATCTTTACTTC	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29054_Pcan_Bot2011_IGS	PGI-B10	1	BD29054_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B10	IGS	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	CGTAATCCTCAGTACTGGCTA	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29054_Pper_Dia2013_COI	PGI-B10	1	BD29054_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	CTTAGTGGCATTAATTCGDAATCC	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29054_Sspa_Oph2007_COI	PGI-B10	1	BD29054_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	GAAGGCCTTCCGATGATC	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29054_Upin_Bot2011_COI	PGI-B10	1	BD29054_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	CTTTAATTACAGCGTTTTTATTGTTGT	137.77167
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29055_Cjap_Sim2018_COI	PGI-B09	1	BD29055_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29055_Esin_And2018_Cyb	PGI-B09	1	BD29055_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B09	Cytb	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	ACCCCTCCTCATATCCAACCA	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29055_Mgig_Bot2012_COI	PGI-B09	1	BD29055_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	TCTTATTCGTTGGAGACTTTATAACCCT	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29055_Mjap_Gib2022_COI	PGI-B09	1	BD29055_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	AATTTAATTGTGACTAGGCATGGTCTG	137.76986
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29056_Cmae_Bot2011_COI	PGI-B08	1	BD29056_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	ATGAACAGTCTATCCTCCTTTAG	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29056_Hsan_Wil2021_COI	PGI-B08	1	BD29056_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	CCTGGGCCGGTATAGTAGGT	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29056_Mros_Gib2022_COI	PGI-B08	1	BD29056_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29056_Msal_Bot2012_COI	PGI-B08	1	BD29056_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	GYTAGTTCCRATGATGTTAGCTG	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29056_Mstr_Wil2021_COI	PGI-B08	1	BD29056_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	GTAGTGGTAACTACTCATGCTTTA	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29056_Pvir_Dia2013_COI	PGI-B08	1	BD29056_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	CTTAGTGGCATTAATTCGDAATCC	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29056_Rhar_Sim2018_COI	PGI-B08	1	BD29056_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	CTGCTGCTATTGCTCATG	137.76814
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29058_Aamu_Bax2006_COI	PGI-B06	1	BD29058_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	GCACAACCGGGATCTTTACTTC	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29058_Pcan_Bot2011_IGS	PGI-B06	1	BD29058_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B06	IGS	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	CGTAATCCTCAGTACTGGCTA	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29058_Pper_Dia2013_COI	PGI-B06	1	BD29058_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	CTTAGTGGCATTAATTCGDAATCC	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29058_Sspa_Oph2007_COI	PGI-B06	1	BD29058_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	GAAGGCCTTCCGATGATC	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29058_Upin_Bot2011_COI	PGI-B06	1	BD29058_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	CTTTAATTACAGCGTTTTTATTGTTGT	137.76476
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29059_Cjap_Sim2018_COI	PGI-B05	1	BD29059_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29059_Esin_And2018_Cyb	PGI-B05	1	BD29059_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B05	Cytb	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	ACCCCTCCTCATATCCAACCA	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29059_Mgig_Bot2012_COI	PGI-B05	1	BD29059_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	TCTTATTCGTTGGAGACTTTATAACCCT	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29059_Mjap_Gib2022_COI	PGI-B05	1	BD29059_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	AATTTAATTGTGACTAGGCATGGTCTG	137.77076
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29061_Aamu_Bax2006_COI	PGI-B03	1	BD29061_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	GCACAACCGGGATCTTTACTTC	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29061_Pcan_Bot2011_IGS	PGI-B03	1	BD29061_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B03	IGS	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	CGTAATCCTCAGTACTGGCTA	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29061_Pper_Dia2013_COI	PGI-B03	1	BD29061_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	CTTAGTGGCATTAATTCGDAATCC	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29061_Sspa_Oph2007_COI	PGI-B03	1	BD29061_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	GAAGGCCTTCCGATGATC	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29061_Upin_Bot2011_COI	PGI-B03	1	BD29061_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	CTTTAATTACAGCGTTTTTATTGTTGT	137.76729
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29062_Cjap_Sim2018_COI	PGI-B02	1	BD29062_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29062_Esin_And2018_Cyb	PGI-B02	1	BD29062_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B02	Cytb	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	ACCCCTCCTCATATCCAACCA	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29062_Mgig_Bot2012_COI	PGI-B02	1	BD29062_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	TCTTATTCGTTGGAGACTTTATAACCCT	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29062_Mjap_Gib2022_COI	PGI-B02	1	BD29062_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	AATTTAATTGTGACTAGGCATGGTCTG	137.76554
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29063_Cmae_Bot2011_COI	PGI-B01	1	BD29063_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	ATGAACAGTCTATCCTCCTTTAG	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29063_Hsan_Wil2021_COI	PGI-B01	1	BD29063_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	CCTGGGCCGGTATAGTAGGT	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29063_Mros_Gib2022_COI	PGI-B01	1	BD29063_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29063_Msal_Bot2012_COI	PGI-B01	1	BD29063_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	GYTAGTTCCRATGATGTTAGCTG	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29063_Mstr_Wil2021_COI	PGI-B01	1	BD29063_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	GTAGTGGTAACTACTCATGCTTTA	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29063_Pvir_Dia2013_COI	PGI-B01	1	BD29063_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	CTTAGTGGCATTAATTCGDAATCC	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29063_Rhar_Sim2018_COI	PGI-B01	1	BD29063_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	CTGCTGCTATTGCTCATG	137.76396
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29065_Aamu_Bax2006_COI	KPT-B34	1	BD29065_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	GCACAACCGGGATCTTTACTTC	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29065_Pcan_Bot2011_IGS	KPT-B34	1	BD29065_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B34	IGS	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	CGTAATCCTCAGTACTGGCTA	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29065_Pper_Dia2013_COI	KPT-B34	1	BD29065_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	CTTAGTGGCATTAATTCGDAATCC	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29065_Sspa_Oph2007_COI	KPT-B34	1	BD29065_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	GAAGGCCTTCCGATGATC	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29065_Upin_Bot2011_COI	KPT-B34	1	BD29065_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	CTTTAATTACAGCGTTTTTATTGTTGT	137.77922
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29066_Cjap_Sim2018_COI	KPT-B33	1	BD29066_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29066_Esin_And2018_Cyb	KPT-B33	1	BD29066_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B33	Cytb	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	ACCCCTCCTCATATCCAACCA	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29066_Mgig_Bot2012_COI	KPT-B33	1	BD29066_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	TCTTATTCGTTGGAGACTTTATAACCCT	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29066_Mjap_Gib2022_COI	KPT-B33	1	BD29066_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	AATTTAATTGTGACTAGGCATGGTCTG	137.77795
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29067_Cmae_Bot2011_COI	KPT-B32	1	BD29067_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	ATGAACAGTCTATCCTCCTTTAG	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29067_Hsan_Wil2021_COI	KPT-B32	1	BD29067_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	CCTGGGCCGGTATAGTAGGT	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29067_Mros_Gib2022_COI	KPT-B32	1	BD29067_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29067_Msal_Bot2012_COI	KPT-B32	1	BD29067_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	GYTAGTTCCRATGATGTTAGCTG	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29067_Mstr_Wil2021_COI	KPT-B32	1	BD29067_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	GTAGTGGTAACTACTCATGCTTTA	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29067_Pvir_Dia2013_COI	KPT-B32	1	BD29067_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	CTTAGTGGCATTAATTCGDAATCC	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29067_Rhar_Sim2018_COI	KPT-B32	1	BD29067_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	CTGCTGCTATTGCTCATG	137.77676
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29069_Aamu_Bax2006_COI	KPT-B30	1	BD29069_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	GCACAACCGGGATCTTTACTTC	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29069_Pcan_Bot2011_IGS	KPT-B30	1	BD29069_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B30	IGS	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	CGTAATCCTCAGTACTGGCTA	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29069_Pper_Dia2013_COI	KPT-B30	1	BD29069_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	CTTAGTGGCATTAATTCGDAATCC	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29069_Sspa_Oph2007_COI	KPT-B30	1	BD29069_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	GAAGGCCTTCCGATGATC	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29069_Upin_Bot2011_COI	KPT-B30	1	BD29069_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	CTTTAATTACAGCGTTTTTATTGTTGT	137.77857
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29070_Cmae_Bot2011_COI	KPT-B29	1	BD29070_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	ATGAACAGTCTATCCTCCTTTAG	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29070_Hsan_Wil2021_COI	KPT-B29	1	BD29070_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	CCTGGGCCGGTATAGTAGGT	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29070_Mros_Gib2022_COI	KPT-B29	1	BD29070_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29070_Msal_Bot2012_COI	KPT-B29	1	BD29070_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	GYTAGTTCCRATGATGTTAGCTG	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29070_Mstr_Wil2021_COI	KPT-B29	1	BD29070_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	GTAGTGGTAACTACTCATGCTTTA	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29070_Pvir_Dia2013_COI	KPT-B29	1	BD29070_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	CTTAGTGGCATTAATTCGDAATCC	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29070_Rhar_Sim2018_COI	KPT-B29	1	BD29070_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	CTGCTGCTATTGCTCATG	137.7774
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29072_Aamu_Bax2006_COI	KPT-B27	1	BD29072_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	GCACAACCGGGATCTTTACTTC	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29072_Pcan_Bot2011_IGS	KPT-B27	1	BD29072_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B27	IGS	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	CGTAATCCTCAGTACTGGCTA	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29072_Pper_Dia2013_COI	KPT-B27	1	BD29072_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	CTTAGTGGCATTAATTCGDAATCC	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29072_Sspa_Oph2007_COI	KPT-B27	1	BD29072_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	GAAGGCCTTCCGATGATC	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29072_Upin_Bot2011_COI	KPT-B27	1	BD29072_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	CTTTAATTACAGCGTTTTTATTGTTGT	137.77929
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29073_Cjap_Sim2018_COI	KPT-B26	1	BD29073_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29073_Esin_And2018_Cyb	KPT-B26	1	BD29073_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B26	Cytb	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	ACCCCTCCTCATATCCAACCA	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29073_Mgig_Bot2012_COI	KPT-B26	1	BD29073_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	TCTTATTCGTTGGAGACTTTATAACCCT	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29073_Mjap_Gib2022_COI	KPT-B26	1	BD29073_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	AATTTAATTGTGACTAGGCATGGTCTG	137.77788
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29074_Cmae_Bot2011_COI	KPT-B25	1	BD29074_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	ATGAACAGTCTATCCTCCTTTAG	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29074_Hsan_Wil2021_COI	KPT-B25	1	BD29074_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	CCTGGGCCGGTATAGTAGGT	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29074_Mros_Gib2022_COI	KPT-B25	1	BD29074_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29074_Msal_Bot2012_COI	KPT-B25	1	BD29074_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	GYTAGTTCCRATGATGTTAGCTG	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29074_Mstr_Wil2021_COI	KPT-B25	1	BD29074_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	GTAGTGGTAACTACTCATGCTTTA	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29074_Pvir_Dia2013_COI	KPT-B25	1	BD29074_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	CTTAGTGGCATTAATTCGDAATCC	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29074_Rhar_Sim2018_COI	KPT-B25	1	BD29074_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	CTGCTGCTATTGCTCATG	137.77663
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29076_Aamu_Bax2006_COI	KPT-B23	1	BD29076_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	GCACAACCGGGATCTTTACTTC	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29076_Pcan_Bot2011_IGS	KPT-B23	1	BD29076_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B23	IGS	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	CGTAATCCTCAGTACTGGCTA	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29076_Pper_Dia2013_COI	KPT-B23	1	BD29076_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	CTTAGTGGCATTAATTCGDAATCC	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29076_Sspa_Oph2007_COI	KPT-B23	1	BD29076_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	GAAGGCCTTCCGATGATC	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29076_Upin_Bot2011_COI	KPT-B23	1	BD29076_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	CTTTAATTACAGCGTTTTTATTGTTGT	137.77717
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29077_Cjap_Sim2018_COI	KPT-B22	1	BD29077_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29077_Esin_And2018_Cyb	KPT-B22	1	BD29077_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B22	Cytb	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	ACCCCTCCTCATATCCAACCA	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29077_Mgig_Bot2012_COI	KPT-B22	1	BD29077_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	TCTTATTCGTTGGAGACTTTATAACCCT	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29077_Mjap_Gib2022_COI	KPT-B22	1	BD29077_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	AATTTAATTGTGACTAGGCATGGTCTG	137.77776
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29078_Cmae_Bot2011_COI	KPT-B21	1	BD29078_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	ATGAACAGTCTATCCTCCTTTAG	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29078_Hsan_Wil2021_COI	KPT-B21	1	BD29078_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	CCTGGGCCGGTATAGTAGGT	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29078_Mros_Gib2022_COI	KPT-B21	1	BD29078_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29078_Msal_Bot2012_COI	KPT-B21	1	BD29078_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	GYTAGTTCCRATGATGTTAGCTG	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29078_Mstr_Wil2021_COI	KPT-B21	1	BD29078_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	GTAGTGGTAACTACTCATGCTTTA	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29078_Pvir_Dia2013_COI	KPT-B21	1	BD29078_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	CTTAGTGGCATTAATTCGDAATCC	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29078_Rhar_Sim2018_COI	KPT-B21	1	BD29078_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	CTGCTGCTATTGCTCATG	137.77675
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29080_Cjap_Sim2018_COI	KPT-B19	1	BD29080_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29080_Esin_And2018_Cyb	KPT-B19	1	BD29080_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B19	Cytb	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	ACCCCTCCTCATATCCAACCA	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29080_Mgig_Bot2012_COI	KPT-B19	1	BD29080_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	TCTTATTCGTTGGAGACTTTATAACCCT	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29080_Mjap_Gib2022_COI	KPT-B19	1	BD29080_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	AATTTAATTGTGACTAGGCATGGTCTG	137.77857
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29081_Cmae_Bot2011_COI	KPT-B18	1	BD29081_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	ATGAACAGTCTATCCTCCTTTAG	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29081_Hsan_Wil2021_COI	KPT-B18	1	BD29081_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	CCTGGGCCGGTATAGTAGGT	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29081_Mros_Gib2022_COI	KPT-B18	1	BD29081_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29081_Msal_Bot2012_COI	KPT-B18	1	BD29081_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	GYTAGTTCCRATGATGTTAGCTG	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29081_Mstr_Wil2021_COI	KPT-B18	1	BD29081_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	GTAGTGGTAACTACTCATGCTTTA	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29081_Pvir_Dia2013_COI	KPT-B18	1	BD29081_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	CTTAGTGGCATTAATTCGDAATCC	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29081_Rhar_Sim2018_COI	KPT-B18	1	BD29081_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	CTGCTGCTATTGCTCATG	137.77734
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29083_Aamu_Bax2006_COI	KPT-B16	1	BD29083_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	GCACAACCGGGATCTTTACTTC	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29083_Pcan_Bot2011_IGS	KPT-B16	1	BD29083_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B16	IGS	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	CGTAATCCTCAGTACTGGCTA	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29083_Pper_Dia2013_COI	KPT-B16	1	BD29083_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	CTTAGTGGCATTAATTCGDAATCC	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29083_Sspa_Oph2007_COI	KPT-B16	1	BD29083_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	GAAGGCCTTCCGATGATC	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29083_Upin_Bot2011_COI	KPT-B16	1	BD29083_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	CTTTAATTACAGCGTTTTTATTGTTGT	137.78061
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29084_Cjap_Sim2018_COI	KPT-B15	1	BD29084_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29084_Esin_And2018_Cyb	KPT-B15	1	BD29084_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B15	Cytb	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	ACCCCTCCTCATATCCAACCA	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29084_Mgig_Bot2012_COI	KPT-B15	1	BD29084_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	TCTTATTCGTTGGAGACTTTATAACCCT	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29084_Mjap_Gib2022_COI	KPT-B15	1	BD29084_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	AATTTAATTGTGACTAGGCATGGTCTG	137.77929
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29085_Cmae_Bot2011_COI	KPT-B14	1	BD29085_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	ATGAACAGTCTATCCTCCTTTAG	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29085_Hsan_Wil2021_COI	KPT-B14	1	BD29085_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	CCTGGGCCGGTATAGTAGGT	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29085_Mros_Gib2022_COI	KPT-B14	1	BD29085_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29085_Msal_Bot2012_COI	KPT-B14	1	BD29085_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	GYTAGTTCCRATGATGTTAGCTG	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29085_Mstr_Wil2021_COI	KPT-B14	1	BD29085_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	GTAGTGGTAACTACTCATGCTTTA	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29085_Pvir_Dia2013_COI	KPT-B14	1	BD29085_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	CTTAGTGGCATTAATTCGDAATCC	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29085_Rhar_Sim2018_COI	KPT-B14	1	BD29085_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	CTGCTGCTATTGCTCATG	137.77798
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29087_Aamu_Bax2006_COI	KPT-B12	1	BD29087_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	GCACAACCGGGATCTTTACTTC	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29087_Pcan_Bot2011_IGS	KPT-B12	1	BD29087_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B12	IGS	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	CGTAATCCTCAGTACTGGCTA	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29087_Pper_Dia2013_COI	KPT-B12	1	BD29087_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	CTTAGTGGCATTAATTCGDAATCC	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29087_Sspa_Oph2007_COI	KPT-B12	1	BD29087_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	GAAGGCCTTCCGATGATC	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29087_Upin_Bot2011_COI	KPT-B12	1	BD29087_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	CTTTAATTACAGCGTTTTTATTGTTGT	137.7754
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29088_Cjap_Sim2018_COI	KPT-B11	1	BD29088_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29088_Esin_And2018_Cyb	KPT-B11	1	BD29088_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B11	Cytb	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	ACCCCTCCTCATATCCAACCA	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29088_Mgig_Bot2012_COI	KPT-B11	1	BD29088_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	TCTTATTCGTTGGAGACTTTATAACCCT	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29088_Mjap_Gib2022_COI	KPT-B11	1	BD29088_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	AATTTAATTGTGACTAGGCATGGTCTG	137.77407
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29089_Cmae_Bot2011_COI	KPT-B10	1	BD29089_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	ATGAACAGTCTATCCTCCTTTAG	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29089_Hsan_Wil2021_COI	KPT-B10	1	BD29089_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	CCTGGGCCGGTATAGTAGGT	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29089_Mros_Gib2022_COI	KPT-B10	1	BD29089_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29089_Msal_Bot2012_COI	KPT-B10	1	BD29089_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	GYTAGTTCCRATGATGTTAGCTG	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29089_Mstr_Wil2021_COI	KPT-B10	1	BD29089_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	GTAGTGGTAACTACTCATGCTTTA	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29089_Pvir_Dia2013_COI	KPT-B10	1	BD29089_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	CTTAGTGGCATTAATTCGDAATCC	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29089_Rhar_Sim2018_COI	KPT-B10	1	BD29089_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	CTGCTGCTATTGCTCATG	137.78008
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29090_Aamu_Bax2006_COI	KPT-B09	1	BD29090_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	GCACAACCGGGATCTTTACTTC	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29090_Pcan_Bot2011_IGS	KPT-B09	1	BD29090_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B09	IGS	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	CGTAATCCTCAGTACTGGCTA	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29090_Pper_Dia2013_COI	KPT-B09	1	BD29090_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	CTTAGTGGCATTAATTCGDAATCC	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29090_Sspa_Oph2007_COI	KPT-B09	1	BD29090_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	GAAGGCCTTCCGATGATC	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29090_Upin_Bot2011_COI	KPT-B09	1	BD29090_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	CTTTAATTACAGCGTTTTTATTGTTGT	137.77871
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29091_Cjap_Sim2018_COI	KPT-B08	1	BD29091_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29091_Esin_And2018_Cyb	KPT-B08	1	BD29091_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B08	Cytb	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	ACCCCTCCTCATATCCAACCA	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29091_Mgig_Bot2012_COI	KPT-B08	1	BD29091_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	TCTTATTCGTTGGAGACTTTATAACCCT	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29091_Mjap_Gib2022_COI	KPT-B08	1	BD29091_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	AATTTAATTGTGACTAGGCATGGTCTG	137.77739
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29092_Cmae_Bot2011_COI	KPT-B07	1	BD29092_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	ATGAACAGTCTATCCTCCTTTAG	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29092_Hsan_Wil2021_COI	KPT-B07	1	BD29092_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	CCTGGGCCGGTATAGTAGGT	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29092_Mros_Gib2022_COI	KPT-B07	1	BD29092_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29092_Msal_Bot2012_COI	KPT-B07	1	BD29092_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	GYTAGTTCCRATGATGTTAGCTG	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29092_Mstr_Wil2021_COI	KPT-B07	1	BD29092_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	GTAGTGGTAACTACTCATGCTTTA	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29092_Pvir_Dia2013_COI	KPT-B07	1	BD29092_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	CTTAGTGGCATTAATTCGDAATCC	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29092_Rhar_Sim2018_COI	KPT-B07	1	BD29092_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	CTGCTGCTATTGCTCATG	137.77614
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29094_Aamu_Bax2006_COI	KPT-B05	1	BD29094_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	GCACAACCGGGATCTTTACTTC	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29094_Pcan_Bot2011_IGS	KPT-B05	1	BD29094_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B05	IGS	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	CGTAATCCTCAGTACTGGCTA	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29094_Pper_Dia2013_COI	KPT-B05	1	BD29094_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	CTTAGTGGCATTAATTCGDAATCC	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29094_Sspa_Oph2007_COI	KPT-B05	1	BD29094_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	GAAGGCCTTCCGATGATC	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29094_Upin_Bot2011_COI	KPT-B05	1	BD29094_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	CTTTAATTACAGCGTTTTTATTGTTGT	137.77363
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29095_Cjap_Sim2018_COI	KPT-B04	1	BD29095_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29095_Esin_And2018_Cyb	KPT-B04	1	BD29095_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B04	Cytb	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	ACCCCTCCTCATATCCAACCA	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29095_Mgig_Bot2012_COI	KPT-B04	1	BD29095_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	TCTTATTCGTTGGAGACTTTATAACCCT	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29095_Mjap_Gib2022_COI	KPT-B04	1	BD29095_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	AATTTAATTGTGACTAGGCATGGTCTG	137.77813
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29096_Cmae_Bot2011_COI	KPT-B03	1	BD29096_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	ATGAACAGTCTATCCTCCTTTAG	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29096_Hsan_Wil2021_COI	KPT-B03	1	BD29096_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	CCTGGGCCGGTATAGTAGGT	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29096_Mros_Gib2022_COI	KPT-B03	1	BD29096_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29096_Msal_Bot2012_COI	KPT-B03	1	BD29096_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	GYTAGTTCCRATGATGTTAGCTG	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29096_Mstr_Wil2021_COI	KPT-B03	1	BD29096_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	GTAGTGGTAACTACTCATGCTTTA	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29096_Pvir_Dia2013_COI	KPT-B03	1	BD29096_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	CTTAGTGGCATTAATTCGDAATCC	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29096_Rhar_Sim2018_COI	KPT-B03	1	BD29096_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	CTGCTGCTATTGCTCATG	137.77684
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29098_Aamu_Bax2006_COI	KPT-B01	1	BD29098_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	GCACAACCGGGATCTTTACTTC	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29098_Pcan_Bot2011_IGS	KPT-B01	1	BD29098_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B01	IGS	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	CGTAATCCTCAGTACTGGCTA	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29098_Pper_Dia2013_COI	KPT-B01	1	BD29098_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	CTTAGTGGCATTAATTCGDAATCC	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29098_Sspa_Oph2007_COI	KPT-B01	1	BD29098_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	GAAGGCCTTCCGATGATC	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29098_Upin_Bot2011_COI	KPT-B01	1	BD29098_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	CTTTAATTACAGCGTTTTTATTGTTGT	137.77442
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29099_Cjap_Sim2018_COI	PLO-B35	1	BD29099_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29099_Esin_And2018_Cyb	PLO-B35	1	BD29099_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B35	Cytb	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	ACCCCTCCTCATATCCAACCA	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29099_Mgig_Bot2012_COI	PLO-B35	1	BD29099_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	TCTTATTCGTTGGAGACTTTATAACCCT	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29099_Mjap_Gib2022_COI	PLO-B35	1	BD29099_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	AATTTAATTGTGACTAGGCATGGTCTG	135.87716
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29100_Cmae_Bot2011_COI	PLO-B34	1	BD29100_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	ATGAACAGTCTATCCTCCTTTAG	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29100_Hsan_Wil2021_COI	PLO-B34	1	BD29100_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	CCTGGGCCGGTATAGTAGGT	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29100_Mros_Gib2022_COI	PLO-B34	1	BD29100_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29100_Msal_Bot2012_COI	PLO-B34	1	BD29100_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	GYTAGTTCCRATGATGTTAGCTG	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29100_Mstr_Wil2021_COI	PLO-B34	1	BD29100_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	GTAGTGGTAACTACTCATGCTTTA	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29100_Pvir_Dia2013_COI	PLO-B34	1	BD29100_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	CTTAGTGGCATTAATTCGDAATCC	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29100_Rhar_Sim2018_COI	PLO-B34	1	BD29100_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	CTGCTGCTATTGCTCATG	135.87284
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29102_Aamu_Bax2006_COI	PLO-B32	1	BD29102_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	GCACAACCGGGATCTTTACTTC	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29102_Pcan_Bot2011_IGS	PLO-B32	1	BD29102_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B32	IGS	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	CGTAATCCTCAGTACTGGCTA	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29102_Pper_Dia2013_COI	PLO-B32	1	BD29102_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	CTTAGTGGCATTAATTCGDAATCC	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29102_Sspa_Oph2007_COI	PLO-B32	1	BD29102_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	GAAGGCCTTCCGATGATC	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29102_Upin_Bot2011_COI	PLO-B32	1	BD29102_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	CTTTAATTACAGCGTTTTTATTGTTGT	135.86825
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29103_Cjap_Sim2018_COI	PLO-B31	1	BD29103_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29103_Esin_And2018_Cyb	PLO-B31	1	BD29103_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B31	Cytb	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	ACCCCTCCTCATATCCAACCA	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29103_Mgig_Bot2012_COI	PLO-B31	1	BD29103_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	TCTTATTCGTTGGAGACTTTATAACCCT	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29103_Mjap_Gib2022_COI	PLO-B31	1	BD29103_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	AATTTAATTGTGACTAGGCATGGTCTG	135.86615
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29104_Cmae_Bot2011_COI	PLO-B30	1	BD29104_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	ATGAACAGTCTATCCTCCTTTAG	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29104_Hsan_Wil2021_COI	PLO-B30	1	BD29104_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	CCTGGGCCGGTATAGTAGGT	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29104_Mros_Gib2022_COI	PLO-B30	1	BD29104_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29104_Msal_Bot2012_COI	PLO-B30	1	BD29104_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	GYTAGTTCCRATGATGTTAGCTG	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29104_Mstr_Wil2021_COI	PLO-B30	1	BD29104_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	GTAGTGGTAACTACTCATGCTTTA	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29104_Pvir_Dia2013_COI	PLO-B30	1	BD29104_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	CTTAGTGGCATTAATTCGDAATCC	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29104_Rhar_Sim2018_COI	PLO-B30	1	BD29104_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	CTGCTGCTATTGCTCATG	135.86831
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29106_Aamu_Bax2006_COI	PLO-B28	1	BD29106_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	GCACAACCGGGATCTTTACTTC	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29106_Pcan_Bot2011_IGS	PLO-B28	1	BD29106_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B28	IGS	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	CGTAATCCTCAGTACTGGCTA	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29106_Pper_Dia2013_COI	PLO-B28	1	BD29106_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	CTTAGTGGCATTAATTCGDAATCC	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29106_Sspa_Oph2007_COI	PLO-B28	1	BD29106_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	GAAGGCCTTCCGATGATC	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29106_Upin_Bot2011_COI	PLO-B28	1	BD29106_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	CTTTAATTACAGCGTTTTTATTGTTGT	135.86504
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29107_Cjap_Sim2018_COI	PLO-B27	1	BD29107_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29107_Esin_And2018_Cyb	PLO-B27	1	BD29107_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B27	Cytb	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	ACCCCTCCTCATATCCAACCA	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29107_Mgig_Bot2012_COI	PLO-B27	1	BD29107_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	TCTTATTCGTTGGAGACTTTATAACCCT	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29107_Mjap_Gib2022_COI	PLO-B27	1	BD29107_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	AATTTAATTGTGACTAGGCATGGTCTG	135.86401
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29108_Cmae_Bot2011_COI	PLO-B26	1	BD29108_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	ATGAACAGTCTATCCTCCTTTAG	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29108_Hsan_Wil2021_COI	PLO-B26	1	BD29108_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	CCTGGGCCGGTATAGTAGGT	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29108_Mros_Gib2022_COI	PLO-B26	1	BD29108_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29108_Msal_Bot2012_COI	PLO-B26	1	BD29108_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	GYTAGTTCCRATGATGTTAGCTG	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29108_Mstr_Wil2021_COI	PLO-B26	1	BD29108_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	GTAGTGGTAACTACTCATGCTTTA	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29108_Pvir_Dia2013_COI	PLO-B26	1	BD29108_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	CTTAGTGGCATTAATTCGDAATCC	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29108_Rhar_Sim2018_COI	PLO-B26	1	BD29108_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	CTGCTGCTATTGCTCATG	135.86628
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29110_Cjap_Sim2018_COI	PLO-B24	1	BD29110_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29110_Esin_And2018_Cyb	PLO-B24	1	BD29110_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B24	Cytb	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	ACCCCTCCTCATATCCAACCA	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29110_Mgig_Bot2012_COI	PLO-B24	1	BD29110_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	TCTTATTCGTTGGAGACTTTATAACCCT	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29110_Mjap_Gib2022_COI	PLO-B24	1	BD29110_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	AATTTAATTGTGACTAGGCATGGTCTG	135.86741
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29111_Cmae_Bot2011_COI	PLO-B23	1	BD29111_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	ATGAACAGTCTATCCTCCTTTAG	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29111_Hsan_Wil2021_COI	PLO-B23	1	BD29111_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	CCTGGGCCGGTATAGTAGGT	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29111_Mros_Gib2022_COI	PLO-B23	1	BD29111_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29111_Msal_Bot2012_COI	PLO-B23	1	BD29111_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	GYTAGTTCCRATGATGTTAGCTG	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29111_Mstr_Wil2021_COI	PLO-B23	1	BD29111_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	GTAGTGGTAACTACTCATGCTTTA	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29111_Pvir_Dia2013_COI	PLO-B23	1	BD29111_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	CTTAGTGGCATTAATTCGDAATCC	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29111_Rhar_Sim2018_COI	PLO-B23	1	BD29111_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	CTGCTGCTATTGCTCATG	135.86511
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29113_Aamu_Bax2006_COI	PLO-B21	1	BD29113_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	GCACAACCGGGATCTTTACTTC	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29113_Pcan_Bot2011_IGS	PLO-B21	1	BD29113_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B21	IGS	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	CGTAATCCTCAGTACTGGCTA	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29113_Pper_Dia2013_COI	PLO-B21	1	BD29113_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	CTTAGTGGCATTAATTCGDAATCC	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29113_Sspa_Oph2007_COI	PLO-B21	1	BD29113_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	GAAGGCCTTCCGATGATC	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29113_Upin_Bot2011_COI	PLO-B21	1	BD29113_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	CTTTAATTACAGCGTTTTTATTGTTGT	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71159	WGS84	BD29113	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29113_Vgib_Wil2025_COI	PLO-B21	1	BD29113_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	CCCCTCTTTACAAGTTCTTTAGGGT	135.86744
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71153	WGS84	BD29114	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29114_Asen_Wil2025_COI	PLO-B20	1	BD29114_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	GTTGTTGTTACAACACATGCTCTTTTG	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29114_Cjap_Sim2018_COI	PLO-B20	1	BD29114_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29114_Esin_And2018_Cyb	PLO-B20	1	BD29114_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B20	Cytb	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	ACCCCTCCTCATATCCAACCA	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29114_Mgig_Bot2012_COI	PLO-B20	1	BD29114_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	TCTTATTCGTTGGAGACTTTATAACCCT	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29114_Mjap_Gib2022_COI	PLO-B20	1	BD29114_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	AATTTAATTGTGACTAGGCATGGTCTG	135.8694
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29115_Cmae_Bot2011_COI	PLO-B19	1	BD29115_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	ATGAACAGTCTATCCTCCTTTAG	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29115_Hsan_Wil2021_COI	PLO-B19	1	BD29115_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	CCTGGGCCGGTATAGTAGGT	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29115_Mros_Gib2022_COI	PLO-B19	1	BD29115_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29115_Msal_Bot2012_COI	PLO-B19	1	BD29115_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	GYTAGTTCCRATGATGTTAGCTG	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29115_Mstr_Wil2021_COI	PLO-B19	1	BD29115_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	GTAGTGGTAACTACTCATGCTTTA	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29115_Pvir_Dia2013_COI	PLO-B19	1	BD29115_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	CTTAGTGGCATTAATTCGDAATCC	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29115_Rhar_Sim2018_COI	PLO-B19	1	BD29115_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	CTGCTGCTATTGCTCATG	135.87177
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29117_Aamu_Bax2006_COI	PLO-B17	1	BD29117_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	GCACAACCGGGATCTTTACTTC	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29117_Pcan_Bot2011_IGS	PLO-B17	1	BD29117_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B17	IGS	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	CGTAATCCTCAGTACTGGCTA	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29117_Pper_Dia2013_COI	PLO-B17	1	BD29117_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	CTTAGTGGCATTAATTCGDAATCC	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29117_Sspa_Oph2007_COI	PLO-B17	1	BD29117_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	GAAGGCCTTCCGATGATC	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29117_Upin_Bot2011_COI	PLO-B17	1	BD29117_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	CTTTAATTACAGCGTTTTTATTGTTGT	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71303	WGS84	BD29117	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29117_Vgib_Wil2025_COI	PLO-B17	1	BD29117_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	CCCCTCTTTACAAGTTCTTTAGGGT	135.87506
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71312	WGS84	BD29118	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29118_Asen_Wil2025_COI	PLO-B16	1	BD29118_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	GTTGTTGTTACAACACATGCTCTTTTG	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29118_Cjap_Sim2018_COI	PLO-B16	1	BD29118_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29118_Esin_And2018_Cyb	PLO-B16	1	BD29118_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B16	Cytb	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	ACCCCTCCTCATATCCAACCA	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29118_Mgig_Bot2012_COI	PLO-B16	1	BD29118_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	TCTTATTCGTTGGAGACTTTATAACCCT	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29118_Mjap_Gib2022_COI	PLO-B16	1	BD29118_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	AATTTAATTGTGACTAGGCATGGTCTG	135.87288
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29119_Cmae_Bot2011_COI	PLO-B15	1	BD29119_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	ATGAACAGTCTATCCTCCTTTAG	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29119_Hsan_Wil2021_COI	PLO-B15	1	BD29119_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	CCTGGGCCGGTATAGTAGGT	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29119_Mros_Gib2022_COI	PLO-B15	1	BD29119_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29119_Msal_Bot2012_COI	PLO-B15	1	BD29119_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	GYTAGTTCCRATGATGTTAGCTG	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29119_Mstr_Wil2021_COI	PLO-B15	1	BD29119_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	GTAGTGGTAACTACTCATGCTTTA	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29119_Pvir_Dia2013_COI	PLO-B15	1	BD29119_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	CTTAGTGGCATTAATTCGDAATCC	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29119_Rhar_Sim2018_COI	PLO-B15	1	BD29119_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	CTGCTGCTATTGCTCATG	135.87184
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29120_Aamu_Bax2006_COI	PLO-B14	1	BD29120_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	GCACAACCGGGATCTTTACTTC	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29120_Pcan_Bot2011_IGS	PLO-B14	1	BD29120_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B14	IGS	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	CGTAATCCTCAGTACTGGCTA	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29120_Pper_Dia2013_COI	PLO-B14	1	BD29120_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	CTTAGTGGCATTAATTCGDAATCC	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29120_Sspa_Oph2007_COI	PLO-B14	1	BD29120_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	GAAGGCCTTCCGATGATC	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29120_Upin_Bot2011_COI	PLO-B14	1	BD29120_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	CTTTAATTACAGCGTTTTTATTGTTGT	135.87404
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29121_Cjap_Sim2018_COI	PLO-B13	1	BD29121_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29121_Esin_And2018_Cyb	PLO-B13	1	BD29121_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B13	Cytb	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	ACCCCTCCTCATATCCAACCA	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29121_Mgig_Bot2012_COI	PLO-B13	1	BD29121_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	TCTTATTCGTTGGAGACTTTATAACCCT	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29121_Mjap_Gib2022_COI	PLO-B13	1	BD29121_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	AATTTAATTGTGACTAGGCATGGTCTG	135.87618
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29122_Cmae_Bot2011_COI	PLO-B12	1	BD29122_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	ATGAACAGTCTATCCTCCTTTAG	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29122_Hsan_Wil2021_COI	PLO-B12	1	BD29122_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	CCTGGGCCGGTATAGTAGGT	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29122_Mros_Gib2022_COI	PLO-B12	1	BD29122_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29122_Msal_Bot2012_COI	PLO-B12	1	BD29122_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	GYTAGTTCCRATGATGTTAGCTG	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29122_Mstr_Wil2021_COI	PLO-B12	1	BD29122_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	GTAGTGGTAACTACTCATGCTTTA	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29122_Pvir_Dia2013_COI	PLO-B12	1	BD29122_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	CTTAGTGGCATTAATTCGDAATCC	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29122_Rhar_Sim2018_COI	PLO-B12	1	BD29122_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	CTGCTGCTATTGCTCATG	135.87842
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29124_Aamu_Bax2006_COI	PLO-B10	1	BD29124_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	GCACAACCGGGATCTTTACTTC	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29124_Pcan_Bot2011_IGS	PLO-B10	1	BD29124_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B10	IGS	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	CGTAATCCTCAGTACTGGCTA	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29124_Pper_Dia2013_COI	PLO-B10	1	BD29124_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	CTTAGTGGCATTAATTCGDAATCC	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29124_Sspa_Oph2007_COI	PLO-B10	1	BD29124_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	GAAGGCCTTCCGATGATC	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29124_Upin_Bot2011_COI	PLO-B10	1	BD29124_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	CTTTAATTACAGCGTTTTTATTGTTGT	135.87733
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29125_Cjap_Sim2018_COI	PLO-B09	1	BD29125_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29125_Esin_And2018_Cyb	PLO-B09	1	BD29125_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B09	Cytb	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	ACCCCTCCTCATATCCAACCA	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29125_Mgig_Bot2012_COI	PLO-B09	1	BD29125_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	TCTTATTCGTTGGAGACTTTATAACCCT	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29125_Mjap_Gib2022_COI	PLO-B09	1	BD29125_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	AATTTAATTGTGACTAGGCATGGTCTG	135.87514
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29126_Cmae_Bot2011_COI	PLO-B08	1	BD29126_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	ATGAACAGTCTATCCTCCTTTAG	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29126_Hsan_Wil2021_COI	PLO-B08	1	BD29126_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	CCTGGGCCGGTATAGTAGGT	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29126_Mros_Gib2022_COI	PLO-B08	1	BD29126_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29126_Msal_Bot2012_COI	PLO-B08	1	BD29126_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	GYTAGTTCCRATGATGTTAGCTG	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29126_Mstr_Wil2021_COI	PLO-B08	1	BD29126_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	GTAGTGGTAACTACTCATGCTTTA	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29126_Pvir_Dia2013_COI	PLO-B08	1	BD29126_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	CTTAGTGGCATTAATTCGDAATCC	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29126_Rhar_Sim2018_COI	PLO-B08	1	BD29126_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	CTGCTGCTATTGCTCATG	135.87292
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29128_Aamu_Bax2006_COI	PLO-B06	1	BD29128_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	GCACAACCGGGATCTTTACTTC	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29128_Pcan_Bot2011_IGS	PLO-B06	1	BD29128_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B06	IGS	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	CGTAATCCTCAGTACTGGCTA	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29128_Pper_Dia2013_COI	PLO-B06	1	BD29128_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	CTTAGTGGCATTAATTCGDAATCC	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.943	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29128_Sspa_Oph2007_COI	PLO-B06	1	BD29128_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	GAAGGCCTTCCGATGATC	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29128_Upin_Bot2011_COI	PLO-B06	1	BD29128_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	CTTTAATTACAGCGTTTTTATTGTTGT	135.8719
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29129_Cjap_Sim2018_COI	PLO-B05	1	BD29129_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29129_Esin_And2018_Cyb	PLO-B05	1	BD29129_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B05	Cytb	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	ACCCCTCCTCATATCCAACCA	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29129_Mgig_Bot2012_COI	PLO-B05	1	BD29129_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	TCTTATTCGTTGGAGACTTTATAACCCT	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29129_Mjap_Gib2022_COI	PLO-B05	1	BD29129_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	AATTTAATTGTGACTAGGCATGGTCTG	135.87293
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29131_Aamu_Bax2006_COI	PLO-B03	1	BD29131_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	GCACAACCGGGATCTTTACTTC	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29131_Pcan_Bot2011_IGS	PLO-B03	1	BD29131_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B03	IGS	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	CGTAATCCTCAGTACTGGCTA	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29131_Pper_Dia2013_COI	PLO-B03	1	BD29131_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	CTTAGTGGCATTAATTCGDAATCC	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29131_Sspa_Oph2007_COI	PLO-B03	1	BD29131_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	GAAGGCCTTCCGATGATC	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29131_Upin_Bot2011_COI	PLO-B03	1	BD29131_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	CTTTAATTACAGCGTTTTTATTGTTGT	135.87627
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29132_Cjap_Sim2018_COI	PLO-B02	1	BD29132_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29132_Esin_And2018_Cyb	PLO-B02	1	BD29132_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B02	Cytb	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	ACCCCTCCTCATATCCAACCA	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29132_Mgig_Bot2012_COI	PLO-B02	1	BD29132_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	TCTTATTCGTTGGAGACTTTATAACCCT	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29132_Mjap_Gib2022_COI	PLO-B02	1	BD29132_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	AATTTAATTGTGACTAGGCATGGTCTG	135.87846
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29133_Cmae_Bot2011_COI	PLO-B01	1	BD29133_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	ATGAACAGTCTATCCTCCTTTAG	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29133_Hsan_Wil2021_COI	PLO-B01	1	BD29133_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	CCTGGGCCGGTATAGTAGGT	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29133_Mros_Gib2022_COI	PLO-B01	1	BD29133_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29133_Msal_Bot2012_COI	PLO-B01	1	BD29133_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	GYTAGTTCCRATGATGTTAGCTG	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29133_Mstr_Wil2021_COI	PLO-B01	1	BD29133_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	GTAGTGGTAACTACTCATGCTTTA	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29133_Pvir_Dia2013_COI	PLO-B01	1	BD29133_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	CTTAGTGGCATTAATTCGDAATCC	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29133_Rhar_Sim2018_COI	PLO-B01	1	BD29133_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	CTGCTGCTATTGCTCATG	135.88088
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29135_Aamu_Bax2006_COI	ADL-A69	1	BD29135_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	GCACAACCGGGATCTTTACTTC	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29135_Pcan_Bot2011_IGS	ADL-A69	1	BD29135_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A69	IGS	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	CGTAATCCTCAGTACTGGCTA	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29135_Pper_Dia2013_COI	ADL-A69	1	BD29135_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	CTTAGTGGCATTAATTCGDAATCC	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.555	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29135_Sspa_Oph2007_COI	ADL-A69	1	BD29135_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	GAAGGCCTTCCGATGATC	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29135_Upin_Bot2011_COI	ADL-A69	1	BD29135_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	CTTTAATTACAGCGTTTTTATTGTTGT	138.51371
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29136_Cjap_Sim2018_COI	ADL-A68	1	BD29136_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29136_Esin_And2018_Cyb	ADL-A68	1	BD29136_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A68	Cytb	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	ACCCCTCCTCATATCCAACCA	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29136_Mgig_Bot2012_COI	ADL-A68	1	BD29136_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	TCTTATTCGTTGGAGACTTTATAACCCT	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29136_Mjap_Gib2022_COI	ADL-A68	1	BD29136_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	AATTTAATTGTGACTAGGCATGGTCTG	138.51366
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.054	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29137_Cmae_Bot2011_COI	ADL-A67	1	BD29137_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	ATGAACAGTCTATCCTCCTTTAG	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29137_Hsan_Wil2021_COI	ADL-A67	1	BD29137_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	CCTGGGCCGGTATAGTAGGT	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29137_Mros_Gib2022_COI	ADL-A67	1	BD29137_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29137_Msal_Bot2012_COI	ADL-A67	1	BD29137_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	GYTAGTTCCRATGATGTTAGCTG	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29137_Mstr_Wil2021_COI	ADL-A67	1	BD29137_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	GTAGTGGTAACTACTCATGCTTTA	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29137_Pvir_Dia2013_COI	ADL-A67	1	BD29137_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	CTTAGTGGCATTAATTCGDAATCC	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29137_Rhar_Sim2018_COI	ADL-A67	1	BD29137_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	CTGCTGCTATTGCTCATG	138.51305
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29139_Aamu_Bax2006_COI	ADL-A65	1	BD29139_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	GCACAACCGGGATCTTTACTTC	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29139_Pcan_Bot2011_IGS	ADL-A65	1	BD29139_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A65	IGS	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	CGTAATCCTCAGTACTGGCTA	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29139_Pper_Dia2013_COI	ADL-A65	1	BD29139_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	CTTAGTGGCATTAATTCGDAATCC	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.481	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29139_Sspa_Oph2007_COI	ADL-A65	1	BD29139_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	GAAGGCCTTCCGATGATC	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29139_Upin_Bot2011_COI	ADL-A65	1	BD29139_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	CTTTAATTACAGCGTTTTTATTGTTGT	138.51317
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	26.735	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29140_Cmae_Bot2011_COI	ADL-A64	1	BD29140_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	ATGAACAGTCTATCCTCCTTTAG	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29140_Hsan_Wil2021_COI	ADL-A64	1	BD29140_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	CCTGGGCCGGTATAGTAGGT	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29140_Mros_Gib2022_COI	ADL-A64	1	BD29140_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29140_Msal_Bot2012_COI	ADL-A64	1	BD29140_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	GYTAGTTCCRATGATGTTAGCTG	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29140_Mstr_Wil2021_COI	ADL-A64	1	BD29140_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	GTAGTGGTAACTACTCATGCTTTA	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29140_Pvir_Dia2013_COI	ADL-A64	1	BD29140_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	CTTAGTGGCATTAATTCGDAATCC	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29140_Rhar_Sim2018_COI	ADL-A64	1	BD29140_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	CTGCTGCTATTGCTCATG	138.51268
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29142_Aamu_Bax2006_COI	ADL-A62	1	BD29142_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	GCACAACCGGGATCTTTACTTC	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29142_Pcan_Bot2011_IGS	ADL-A62	1	BD29142_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A62	IGS	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	CGTAATCCTCAGTACTGGCTA	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29142_Pper_Dia2013_COI	ADL-A62	1	BD29142_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	CTTAGTGGCATTAATTCGDAATCC	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.145	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29142_Sspa_Oph2007_COI	ADL-A62	1	BD29142_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	GAAGGCCTTCCGATGATC	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29142_Upin_Bot2011_COI	ADL-A62	1	BD29142_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	CTTTAATTACAGCGTTTTTATTGTTGT	138.51129
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29143_Cjap_Sim2018_COI	ADL-A61	1	BD29143_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29143_Esin_And2018_Cyb	ADL-A61	1	BD29143_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A61	Cytb	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	ACCCCTCCTCATATCCAACCA	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29143_Mgig_Bot2012_COI	ADL-A61	1	BD29143_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	TCTTATTCGTTGGAGACTTTATAACCCT	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29143_Mjap_Gib2022_COI	ADL-A61	1	BD29143_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	AATTTAATTGTGACTAGGCATGGTCTG	138.51199
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.105	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29144_Cmae_Bot2011_COI	ADL-A60	1	BD29144_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	ATGAACAGTCTATCCTCCTTTAG	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29144_Hsan_Wil2021_COI	ADL-A60	1	BD29144_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	CCTGGGCCGGTATAGTAGGT	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29144_Mros_Gib2022_COI	ADL-A60	1	BD29144_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29144_Msal_Bot2012_COI	ADL-A60	1	BD29144_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	GYTAGTTCCRATGATGTTAGCTG	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29144_Mstr_Wil2021_COI	ADL-A60	1	BD29144_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	GTAGTGGTAACTACTCATGCTTTA	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29144_Pvir_Dia2013_COI	ADL-A60	1	BD29144_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	CTTAGTGGCATTAATTCGDAATCC	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29144_Rhar_Sim2018_COI	ADL-A60	1	BD29144_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	CTGCTGCTATTGCTCATG	138.5106
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29146_Aamu_Bax2006_COI	ADL-A58	1	BD29146_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	GCACAACCGGGATCTTTACTTC	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29146_Pcan_Bot2011_IGS	ADL-A58	1	BD29146_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A58	IGS	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	CGTAATCCTCAGTACTGGCTA	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29146_Pper_Dia2013_COI	ADL-A58	1	BD29146_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	CTTAGTGGCATTAATTCGDAATCC	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.331	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29146_Sspa_Oph2007_COI	ADL-A58	1	BD29146_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	GAAGGCCTTCCGATGATC	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29146_Upin_Bot2011_COI	ADL-A58	1	BD29146_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	CTTTAATTACAGCGTTTTTATTGTTGT	138.51002
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29147_Cjap_Sim2018_COI	ADL-A57	1	BD29147_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29147_Esin_And2018_Cyb	ADL-A57	1	BD29147_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A57	Cytb	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	ACCCCTCCTCATATCCAACCA	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29147_Mgig_Bot2012_COI	ADL-A57	1	BD29147_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	TCTTATTCGTTGGAGACTTTATAACCCT	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29147_Mjap_Gib2022_COI	ADL-A57	1	BD29147_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	AATTTAATTGTGACTAGGCATGGTCTG	138.51067
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29148_Cmae_Bot2011_COI	ADL-A56	1	BD29148_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	ATGAACAGTCTATCCTCCTTTAG	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29148_Hsan_Wil2021_COI	ADL-A56	1	BD29148_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	CCTGGGCCGGTATAGTAGGT	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29148_Mros_Gib2022_COI	ADL-A56	1	BD29148_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29148_Msal_Bot2012_COI	ADL-A56	1	BD29148_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	GYTAGTTCCRATGATGTTAGCTG	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29148_Mstr_Wil2021_COI	ADL-A56	1	BD29148_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	GTAGTGGTAACTACTCATGCTTTA	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29148_Pvir_Dia2013_COI	ADL-A56	1	BD29148_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	CTTAGTGGCATTAATTCGDAATCC	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29148_Rhar_Sim2018_COI	ADL-A56	1	BD29148_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	CTGCTGCTATTGCTCATG	138.50936
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29150_Cjap_Sim2018_COI	ADL-A54	1	BD29150_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29150_Esin_And2018_Cyb	ADL-A54	1	BD29150_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A54	Cytb	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	ACCCCTCCTCATATCCAACCA	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29150_Mgig_Bot2012_COI	ADL-A54	1	BD29150_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	TCTTATTCGTTGGAGACTTTATAACCCT	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29150_Mjap_Gib2022_COI	ADL-A54	1	BD29150_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	AATTTAATTGTGACTAGGCATGGTCTG	138.50873
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	40.493	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29151_Cmae_Bot2011_COI	ADL-A53	1	BD29151_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	ATGAACAGTCTATCCTCCTTTAG	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29151_Hsan_Wil2021_COI	ADL-A53	1	BD29151_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	CCTGGGCCGGTATAGTAGGT	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29151_Mros_Gib2022_COI	ADL-A53	1	BD29151_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29151_Msal_Bot2012_COI	ADL-A53	1	BD29151_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	GYTAGTTCCRATGATGTTAGCTG	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29151_Mstr_Wil2021_COI	ADL-A53	1	BD29151_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	GTAGTGGTAACTACTCATGCTTTA	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29151_Pvir_Dia2013_COI	ADL-A53	1	BD29151_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	CTTAGTGGCATTAATTCGDAATCC	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29151_Rhar_Sim2018_COI	ADL-A53	1	BD29151_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	CTGCTGCTATTGCTCATG	138.5094
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29153_Aamu_Bax2006_COI	ADL-A51	1	BD29153_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	GCACAACCGGGATCTTTACTTC	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29153_Pcan_Bot2011_IGS	ADL-A51	1	BD29153_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A51	IGS	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	CGTAATCCTCAGTACTGGCTA	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29153_Pper_Dia2013_COI	ADL-A51	1	BD29153_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	CTTAGTGGCATTAATTCGDAATCC	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.384	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29153_Sspa_Oph2007_COI	ADL-A51	1	BD29153_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	GAAGGCCTTCCGATGATC	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29153_Upin_Bot2011_COI	ADL-A51	1	BD29153_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	CTTTAATTACAGCGTTTTTATTGTTGT	138.50876
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29154_Cjap_Sim2018_COI	ADL-A50	1	BD29154_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29154_Esin_And2018_Cyb	ADL-A50	1	BD29154_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A50	Cytb	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	ACCCCTCCTCATATCCAACCA	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29154_Mgig_Bot2012_COI	ADL-A50	1	BD29154_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	TCTTATTCGTTGGAGACTTTATAACCCT	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29154_Mjap_Gib2022_COI	ADL-A50	1	BD29154_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	AATTTAATTGTGACTAGGCATGGTCTG	138.5073
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29155_Cmae_Bot2011_COI	ADL-A49	1	BD29155_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	ATGAACAGTCTATCCTCCTTTAG	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29155_Hsan_Wil2021_COI	ADL-A49	1	BD29155_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	CCTGGGCCGGTATAGTAGGT	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29155_Mros_Gib2022_COI	ADL-A49	1	BD29155_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29155_Msal_Bot2012_COI	ADL-A49	1	BD29155_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	GYTAGTTCCRATGATGTTAGCTG	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29155_Mstr_Wil2021_COI	ADL-A49	1	BD29155_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	GTAGTGGTAACTACTCATGCTTTA	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29155_Pvir_Dia2013_COI	ADL-A49	1	BD29155_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	CTTAGTGGCATTAATTCGDAATCC	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29155_Rhar_Sim2018_COI	ADL-A49	1	BD29155_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	CTGCTGCTATTGCTCATG	138.50794
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29157_Aamu_Bax2006_COI	ADL-A47	1	BD29157_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	GCACAACCGGGATCTTTACTTC	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29157_Pcan_Bot2011_IGS	ADL-A47	1	BD29157_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A47	IGS	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	CGTAATCCTCAGTACTGGCTA	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29157_Pper_Dia2013_COI	ADL-A47	1	BD29157_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	CTTAGTGGCATTAATTCGDAATCC	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.009	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29157_Sspa_Oph2007_COI	ADL-A47	1	BD29157_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	GAAGGCCTTCCGATGATC	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29157_Upin_Bot2011_COI	ADL-A47	1	BD29157_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	CTTTAATTACAGCGTTTTTATTGTTGT	138.50732
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29158_Cjap_Sim2018_COI	ADL-A46	1	BD29158_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29158_Esin_And2018_Cyb	ADL-A46	1	BD29158_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A46	Cytb	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	ACCCCTCCTCATATCCAACCA	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29158_Mgig_Bot2012_COI	ADL-A46	1	BD29158_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	TCTTATTCGTTGGAGACTTTATAACCCT	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29158_Mjap_Gib2022_COI	ADL-A46	1	BD29158_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	AATTTAATTGTGACTAGGCATGGTCTG	138.50951
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29159_Cmae_Bot2011_COI	ADL-A45	1	BD29159_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	ATGAACAGTCTATCCTCCTTTAG	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29159_Hsan_Wil2021_COI	ADL-A45	1	BD29159_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	CCTGGGCCGGTATAGTAGGT	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29159_Mros_Gib2022_COI	ADL-A45	1	BD29159_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29159_Msal_Bot2012_COI	ADL-A45	1	BD29159_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	GYTAGTTCCRATGATGTTAGCTG	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29159_Mstr_Wil2021_COI	ADL-A45	1	BD29159_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	GTAGTGGTAACTACTCATGCTTTA	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29159_Pvir_Dia2013_COI	ADL-A45	1	BD29159_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	CTTAGTGGCATTAATTCGDAATCC	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29159_Rhar_Sim2018_COI	ADL-A45	1	BD29159_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	CTGCTGCTATTGCTCATG	138.50813
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29160_Aamu_Bax2006_COI	ADL-A44	1	BD29160_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	GCACAACCGGGATCTTTACTTC	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29160_Pcan_Bot2011_IGS	ADL-A44	1	BD29160_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A44	IGS	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	CGTAATCCTCAGTACTGGCTA	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29160_Pper_Dia2013_COI	ADL-A44	1	BD29160_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	CTTAGTGGCATTAATTCGDAATCC	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.374	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29160_Sspa_Oph2007_COI	ADL-A44	1	BD29160_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	GAAGGCCTTCCGATGATC	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29160_Upin_Bot2011_COI	ADL-A44	1	BD29160_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	CTTTAATTACAGCGTTTTTATTGTTGT	138.50737
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29161_Cjap_Sim2018_COI	ADL-A43	1	BD29161_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29161_Esin_And2018_Cyb	ADL-A43	1	BD29161_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A43	Cytb	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	ACCCCTCCTCATATCCAACCA	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29161_Mgig_Bot2012_COI	ADL-A43	1	BD29161_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	TCTTATTCGTTGGAGACTTTATAACCCT	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29161_Mjap_Gib2022_COI	ADL-A43	1	BD29161_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	AATTTAATTGTGACTAGGCATGGTCTG	138.50739
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.991	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29162_Cmae_Bot2011_COI	ADL-A42	1	BD29162_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	ATGAACAGTCTATCCTCCTTTAG	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29162_Hsan_Wil2021_COI	ADL-A42	1	BD29162_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	CCTGGGCCGGTATAGTAGGT	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29162_Mros_Gib2022_COI	ADL-A42	1	BD29162_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29162_Msal_Bot2012_COI	ADL-A42	1	BD29162_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	GYTAGTTCCRATGATGTTAGCTG	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29162_Mstr_Wil2021_COI	ADL-A42	1	BD29162_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	GTAGTGGTAACTACTCATGCTTTA	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29162_Pvir_Dia2013_COI	ADL-A42	1	BD29162_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	CTTAGTGGCATTAATTCGDAATCC	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29162_Rhar_Sim2018_COI	ADL-A42	1	BD29162_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	CTGCTGCTATTGCTCATG	138.50687
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29164_Aamu_Bax2006_COI	ADL-A40	1	BD29164_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	GCACAACCGGGATCTTTACTTC	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29164_Pcan_Bot2011_IGS	ADL-A40	1	BD29164_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A40	IGS	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	CGTAATCCTCAGTACTGGCTA	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29164_Pper_Dia2013_COI	ADL-A40	1	BD29164_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	CTTAGTGGCATTAATTCGDAATCC	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.324	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29164_Sspa_Oph2007_COI	ADL-A40	1	BD29164_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	GAAGGCCTTCCGATGATC	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29164_Upin_Bot2011_COI	ADL-A40	1	BD29164_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	CTTTAATTACAGCGTTTTTATTGTTGT	138.50533
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29165_Cjap_Sim2018_COI	ADL-A39	1	BD29165_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29165_Esin_And2018_Cyb	ADL-A39	1	BD29165_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A39	Cytb	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	ACCCCTCCTCATATCCAACCA	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29165_Mgig_Bot2012_COI	ADL-A39	1	BD29165_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	TCTTATTCGTTGGAGACTTTATAACCCT	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29165_Mjap_Gib2022_COI	ADL-A39	1	BD29165_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	AATTTAATTGTGACTAGGCATGGTCTG	138.50579
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.385	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29166_Cmae_Bot2011_COI	ADL-A38	1	BD29166_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	ATGAACAGTCTATCCTCCTTTAG	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29166_Hsan_Wil2021_COI	ADL-A38	1	BD29166_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	CCTGGGCCGGTATAGTAGGT	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29166_Mros_Gib2022_COI	ADL-A38	1	BD29166_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29166_Msal_Bot2012_COI	ADL-A38	1	BD29166_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	GYTAGTTCCRATGATGTTAGCTG	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29166_Mstr_Wil2021_COI	ADL-A38	1	BD29166_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	GTAGTGGTAACTACTCATGCTTTA	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29166_Pvir_Dia2013_COI	ADL-A38	1	BD29166_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	CTTAGTGGCATTAATTCGDAATCC	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29166_Rhar_Sim2018_COI	ADL-A38	1	BD29166_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	CTGCTGCTATTGCTCATG	138.50531
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29168_Aamu_Bax2006_COI	ADL-A36	1	BD29168_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	GCACAACCGGGATCTTTACTTC	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29168_Pcan_Bot2011_IGS	ADL-A36	1	BD29168_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A36	IGS	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	CGTAATCCTCAGTACTGGCTA	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29168_Pper_Dia2013_COI	ADL-A36	1	BD29168_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	CTTAGTGGCATTAATTCGDAATCC	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.594	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29168_Sspa_Oph2007_COI	ADL-A36	1	BD29168_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	GAAGGCCTTCCGATGATC	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29168_Upin_Bot2011_COI	ADL-A36	1	BD29168_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	CTTTAATTACAGCGTTTTTATTGTTGT	138.4992
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29169_Cjap_Sim2018_COI	ADL-A35	1	BD29169_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29169_Esin_And2018_Cyb	ADL-A35	1	BD29169_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A35	Cytb	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	ACCCCTCCTCATATCCAACCA	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29169_Mgig_Bot2012_COI	ADL-A35	1	BD29169_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	TCTTATTCGTTGGAGACTTTATAACCCT	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29169_Mjap_Gib2022_COI	ADL-A35	1	BD29169_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	AATTTAATTGTGACTAGGCATGGTCTG	138.5
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29171_Aamu_Bax2006_COI	ADL-A33	1	BD29171_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	GCACAACCGGGATCTTTACTTC	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29171_Pcan_Bot2011_IGS	ADL-A33	1	BD29171_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A33	IGS	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	CGTAATCCTCAGTACTGGCTA	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29171_Pper_Dia2013_COI	ADL-A33	1	BD29171_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	CTTAGTGGCATTAATTCGDAATCC	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29171_Sspa_Oph2007_COI	ADL-A33	1	BD29171_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	GAAGGCCTTCCGATGATC	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29171_Upin_Bot2011_COI	ADL-A33	1	BD29171_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	CTTTAATTACAGCGTTTTTATTGTTGT	138.5011
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29172_Cjap_Sim2018_COI	ADL-A32	1	BD29172_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29172_Esin_And2018_Cyb	ADL-A32	1	BD29172_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A32	Cytb	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	ACCCCTCCTCATATCCAACCA	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29172_Mgig_Bot2012_COI	ADL-A32	1	BD29172_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	TCTTATTCGTTGGAGACTTTATAACCCT	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29172_Mjap_Gib2022_COI	ADL-A32	1	BD29172_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	AATTTAATTGTGACTAGGCATGGTCTG	138.49913
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.586	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29173_Cmae_Bot2011_COI	ADL-A31	1	BD29173_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	ATGAACAGTCTATCCTCCTTTAG	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29173_Hsan_Wil2021_COI	ADL-A31	1	BD29173_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	CCTGGGCCGGTATAGTAGGT	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29173_Mros_Gib2022_COI	ADL-A31	1	BD29173_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29173_Msal_Bot2012_COI	ADL-A31	1	BD29173_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	GYTAGTTCCRATGATGTTAGCTG	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29173_Mstr_Wil2021_COI	ADL-A31	1	BD29173_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	GTAGTGGTAACTACTCATGCTTTA	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29173_Pvir_Dia2013_COI	ADL-A31	1	BD29173_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	CTTAGTGGCATTAATTCGDAATCC	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29173_Rhar_Sim2018_COI	ADL-A31	1	BD29173_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	CTGCTGCTATTGCTCATG	138.49696
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29175_Aamu_Bax2006_COI	ADL-A29	1	BD29175_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	GCACAACCGGGATCTTTACTTC	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29175_Pcan_Bot2011_IGS	ADL-A29	1	BD29175_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A29	IGS	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	CGTAATCCTCAGTACTGGCTA	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29175_Pper_Dia2013_COI	ADL-A29	1	BD29175_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	CTTAGTGGCATTAATTCGDAATCC	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.584	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29175_Sspa_Oph2007_COI	ADL-A29	1	BD29175_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	GAAGGCCTTCCGATGATC	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29175_Upin_Bot2011_COI	ADL-A29	1	BD29175_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	CTTTAATTACAGCGTTTTTATTGTTGT	138.49487
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29176_Cjap_Sim2018_COI	ADL-A28	1	BD29176_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29176_Esin_And2018_Cyb	ADL-A28	1	BD29176_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A28	Cytb	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	ACCCCTCCTCATATCCAACCA	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29176_Mgig_Bot2012_COI	ADL-A28	1	BD29176_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	TCTTATTCGTTGGAGACTTTATAACCCT	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29176_Mjap_Gib2022_COI	ADL-A28	1	BD29176_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	AATTTAATTGTGACTAGGCATGGTCTG	138.49389
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.368	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29177_Cmae_Bot2011_COI	ADL-A27	1	BD29177_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	ATGAACAGTCTATCCTCCTTTAG	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29177_Hsan_Wil2021_COI	ADL-A27	1	BD29177_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	CCTGGGCCGGTATAGTAGGT	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29177_Mros_Gib2022_COI	ADL-A27	1	BD29177_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29177_Msal_Bot2012_COI	ADL-A27	1	BD29177_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	GYTAGTTCCRATGATGTTAGCTG	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29177_Mstr_Wil2021_COI	ADL-A27	1	BD29177_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	GTAGTGGTAACTACTCATGCTTTA	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29177_Pvir_Dia2013_COI	ADL-A27	1	BD29177_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	CTTAGTGGCATTAATTCGDAATCC	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29177_Rhar_Sim2018_COI	ADL-A27	1	BD29177_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	CTGCTGCTATTGCTCATG	138.4918
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29179_Aamu_Bax2006_COI	ADL-A25	1	BD29179_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	GCACAACCGGGATCTTTACTTC	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29179_Pcan_Bot2011_IGS	ADL-A25	1	BD29179_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A25	IGS	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	CGTAATCCTCAGTACTGGCTA	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29179_Pper_Dia2013_COI	ADL-A25	1	BD29179_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	CTTAGTGGCATTAATTCGDAATCC	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.521	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29179_Sspa_Oph2007_COI	ADL-A25	1	BD29179_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	GAAGGCCTTCCGATGATC	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29179_Upin_Bot2011_COI	ADL-A25	1	BD29179_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	CTTTAATTACAGCGTTTTTATTGTTGT	138.49085
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.608	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29180_Cmae_Bot2011_COI	ADL-A24	1	BD29180_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	ATGAACAGTCTATCCTCCTTTAG	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29180_Hsan_Wil2021_COI	ADL-A24	1	BD29180_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	CCTGGGCCGGTATAGTAGGT	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29180_Mros_Gib2022_COI	ADL-A24	1	BD29180_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29180_Msal_Bot2012_COI	ADL-A24	1	BD29180_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	GYTAGTTCCRATGATGTTAGCTG	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29180_Mstr_Wil2021_COI	ADL-A24	1	BD29180_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	GTAGTGGTAACTACTCATGCTTTA	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29180_Pvir_Dia2013_COI	ADL-A24	1	BD29180_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	CTTAGTGGCATTAATTCGDAATCC	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29180_Rhar_Sim2018_COI	ADL-A24	1	BD29180_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	CTGCTGCTATTGCTCATG	138.48685
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29182_Aamu_Bax2006_COI	ADL-A22	1	BD29182_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	GCACAACCGGGATCTTTACTTC	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29182_Pcan_Bot2011_IGS	ADL-A22	1	BD29182_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A22	IGS	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	CGTAATCCTCAGTACTGGCTA	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29182_Pper_Dia2013_COI	ADL-A22	1	BD29182_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	CTTAGTGGCATTAATTCGDAATCC	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.074	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29182_Sspa_Oph2007_COI	ADL-A22	1	BD29182_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	GAAGGCCTTCCGATGATC	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29182_Upin_Bot2011_COI	ADL-A22	1	BD29182_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	CTTTAATTACAGCGTTTTTATTGTTGT	138.48798
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29183_Cjap_Sim2018_COI	ADL-A21	1	BD29183_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29183_Esin_And2018_Cyb	ADL-A21	1	BD29183_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A21	Cytb	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	ACCCCTCCTCATATCCAACCA	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29183_Mgig_Bot2012_COI	ADL-A21	1	BD29183_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	TCTTATTCGTTGGAGACTTTATAACCCT	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29183_Mjap_Gib2022_COI	ADL-A21	1	BD29183_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	AATTTAATTGTGACTAGGCATGGTCTG	138.48607
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29184_Cmae_Bot2011_COI	ADL-A20	1	BD29184_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	ATGAACAGTCTATCCTCCTTTAG	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29184_Hsan_Wil2021_COI	ADL-A20	1	BD29184_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	CCTGGGCCGGTATAGTAGGT	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29184_Mros_Gib2022_COI	ADL-A20	1	BD29184_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29184_Msal_Bot2012_COI	ADL-A20	1	BD29184_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	GYTAGTTCCRATGATGTTAGCTG	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29184_Mstr_Wil2021_COI	ADL-A20	1	BD29184_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	GTAGTGGTAACTACTCATGCTTTA	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29184_Pvir_Dia2013_COI	ADL-A20	1	BD29184_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	CTTAGTGGCATTAATTCGDAATCC	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29184_Rhar_Sim2018_COI	ADL-A20	1	BD29184_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	CTGCTGCTATTGCTCATG	138.4887
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29186_Aamu_Bax2006_COI	ADL-A18	1	BD29186_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	GCACAACCGGGATCTTTACTTC	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29186_Pcan_Bot2011_IGS	ADL-A18	1	BD29186_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A18	IGS	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	CGTAATCCTCAGTACTGGCTA	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29186_Pper_Dia2013_COI	ADL-A18	1	BD29186_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	CTTAGTGGCATTAATTCGDAATCC	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.957	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29186_Sspa_Oph2007_COI	ADL-A18	1	BD29186_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	GAAGGCCTTCCGATGATC	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29186_Upin_Bot2011_COI	ADL-A18	1	BD29186_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	CTTTAATTACAGCGTTTTTATTGTTGT	138.48482
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29187_Cjap_Sim2018_COI	ADL-A17	1	BD29187_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29187_Esin_And2018_Cyb	ADL-A17	1	BD29187_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A17	Cytb	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	ACCCCTCCTCATATCCAACCA	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29187_Mgig_Bot2012_COI	ADL-A17	1	BD29187_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	TCTTATTCGTTGGAGACTTTATAACCCT	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29187_Mjap_Gib2022_COI	ADL-A17	1	BD29187_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	AATTTAATTGTGACTAGGCATGGTCTG	138.48592
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.285	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29188_Cmae_Bot2011_COI	ADL-A16	1	BD29188_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	ATGAACAGTCTATCCTCCTTTAG	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29188_Hsan_Wil2021_COI	ADL-A16	1	BD29188_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	CCTGGGCCGGTATAGTAGGT	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29188_Mros_Gib2022_COI	ADL-A16	1	BD29188_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29188_Msal_Bot2012_COI	ADL-A16	1	BD29188_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	GYTAGTTCCRATGATGTTAGCTG	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29188_Mstr_Wil2021_COI	ADL-A16	1	BD29188_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	GTAGTGGTAACTACTCATGCTTTA	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29188_Pvir_Dia2013_COI	ADL-A16	1	BD29188_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	CTTAGTGGCATTAATTCGDAATCC	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29188_Rhar_Sim2018_COI	ADL-A16	1	BD29188_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	CTGCTGCTATTGCTCATG	138.48413
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29190_Cjap_Sim2018_COI	ADL-A14	1	BD29190_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29190_Esin_And2018_Cyb	ADL-A14	1	BD29190_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A14	Cytb	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	ACCCCTCCTCATATCCAACCA	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29190_Mgig_Bot2012_COI	ADL-A14	1	BD29190_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	TCTTATTCGTTGGAGACTTTATAACCCT	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29190_Mjap_Gib2022_COI	ADL-A14	1	BD29190_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	AATTTAATTGTGACTAGGCATGGTCTG	138.48294
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.992	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29191_Cmae_Bot2011_COI	ADL-A13	1	BD29191_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	ATGAACAGTCTATCCTCCTTTAG	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29191_Hsan_Wil2021_COI	ADL-A13	1	BD29191_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	CCTGGGCCGGTATAGTAGGT	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29191_Mros_Gib2022_COI	ADL-A13	1	BD29191_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29191_Msal_Bot2012_COI	ADL-A13	1	BD29191_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	GYTAGTTCCRATGATGTTAGCTG	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29191_Mstr_Wil2021_COI	ADL-A13	1	BD29191_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	GTAGTGGTAACTACTCATGCTTTA	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29191_Pvir_Dia2013_COI	ADL-A13	1	BD29191_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	CTTAGTGGCATTAATTCGDAATCC	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29191_Rhar_Sim2018_COI	ADL-A13	1	BD29191_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	CTGCTGCTATTGCTCATG	138.48374
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29193_Aamu_Bax2006_COI	ADL-A11	1	BD29193_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	GCACAACCGGGATCTTTACTTC	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29193_Pcan_Bot2011_IGS	ADL-A11	1	BD29193_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A11	IGS	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	CGTAATCCTCAGTACTGGCTA	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29193_Pper_Dia2013_COI	ADL-A11	1	BD29193_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	CTTAGTGGCATTAATTCGDAATCC	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.2	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29193_Sspa_Oph2007_COI	ADL-A11	1	BD29193_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	GAAGGCCTTCCGATGATC	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29193_Upin_Bot2011_COI	ADL-A11	1	BD29193_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	CTTTAATTACAGCGTTTTTATTGTTGT	138.48302
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29194_Cjap_Sim2018_COI	ADL-A10	1	BD29194_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29194_Esin_And2018_Cyb	ADL-A10	1	BD29194_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A10	Cytb	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	ACCCCTCCTCATATCCAACCA	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29194_Mgig_Bot2012_COI	ADL-A10	1	BD29194_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	TCTTATTCGTTGGAGACTTTATAACCCT	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29194_Mjap_Gib2022_COI	ADL-A10	1	BD29194_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	AATTTAATTGTGACTAGGCATGGTCTG	138.4815
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.451	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29195_Cmae_Bot2011_COI	ADL-A09	1	BD29195_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	ATGAACAGTCTATCCTCCTTTAG	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29195_Hsan_Wil2021_COI	ADL-A09	1	BD29195_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	CCTGGGCCGGTATAGTAGGT	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29195_Mros_Gib2022_COI	ADL-A09	1	BD29195_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29195_Msal_Bot2012_COI	ADL-A09	1	BD29195_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	GYTAGTTCCRATGATGTTAGCTG	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29195_Mstr_Wil2021_COI	ADL-A09	1	BD29195_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	GTAGTGGTAACTACTCATGCTTTA	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29195_Pvir_Dia2013_COI	ADL-A09	1	BD29195_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	CTTAGTGGCATTAATTCGDAATCC	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29195_Rhar_Sim2018_COI	ADL-A09	1	BD29195_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	CTGCTGCTATTGCTCATG	138.48218
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29197_Aamu_Bax2006_COI	ADL-A07	1	BD29197_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	GCACAACCGGGATCTTTACTTC	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29197_Pcan_Bot2011_IGS	ADL-A07	1	BD29197_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A07	IGS	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	CGTAATCCTCAGTACTGGCTA	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29197_Pper_Dia2013_COI	ADL-A07	1	BD29197_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	CTTAGTGGCATTAATTCGDAATCC	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.165	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29197_Sspa_Oph2007_COI	ADL-A07	1	BD29197_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	GAAGGCCTTCCGATGATC	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29197_Upin_Bot2011_COI	ADL-A07	1	BD29197_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	CTTTAATTACAGCGTTTTTATTGTTGT	138.4788
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29198_Cjap_Sim2018_COI	ADL-A06	1	BD29198_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29198_Esin_And2018_Cyb	ADL-A06	1	BD29198_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A06	Cytb	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	ACCCCTCCTCATATCCAACCA	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29198_Mgig_Bot2012_COI	ADL-A06	1	BD29198_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	TCTTATTCGTTGGAGACTTTATAACCCT	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29198_Mjap_Gib2022_COI	ADL-A06	1	BD29198_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	AATTTAATTGTGACTAGGCATGGTCTG	138.47728
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.314	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29199_Cmae_Bot2011_COI	ADL-A05	1	BD29199_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	ATGAACAGTCTATCCTCCTTTAG	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29199_Hsan_Wil2021_COI	ADL-A05	1	BD29199_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	CCTGGGCCGGTATAGTAGGT	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29199_Mros_Gib2022_COI	ADL-A05	1	BD29199_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29199_Msal_Bot2012_COI	ADL-A05	1	BD29199_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	GYTAGTTCCRATGATGTTAGCTG	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29199_Mstr_Wil2021_COI	ADL-A05	1	BD29199_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	GTAGTGGTAACTACTCATGCTTTA	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29199_Pvir_Dia2013_COI	ADL-A05	1	BD29199_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	CTTAGTGGCATTAATTCGDAATCC	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29199_Rhar_Sim2018_COI	ADL-A05	1	BD29199_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	CTGCTGCTATTGCTCATG	138.47665
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29201_Aamu_Bax2006_COI	ADL-A03	1	BD29201_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	GCACAACCGGGATCTTTACTTC	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29201_Pcan_Bot2011_IGS	ADL-A03	1	BD29201_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A03	IGS	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	CGTAATCCTCAGTACTGGCTA	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29201_Pper_Dia2013_COI	ADL-A03	1	BD29201_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	CTTAGTGGCATTAATTCGDAATCC	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.401	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29201_Sspa_Oph2007_COI	ADL-A03	1	BD29201_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	GAAGGCCTTCCGATGATC	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29201_Upin_Bot2011_COI	ADL-A03	1	BD29201_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	CTTTAATTACAGCGTTTTTATTGTTGT	138.47409
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29202_Cjap_Sim2018_COI	ADL-A02	1	BD29202_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29202_Esin_And2018_Cyb	ADL-A02	1	BD29202_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A02	Cytb	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	ACCCCTCCTCATATCCAACCA	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29202_Mgig_Bot2012_COI	ADL-A02	1	BD29202_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	TCTTATTCGTTGGAGACTTTATAACCCT	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29202_Mjap_Gib2022_COI	ADL-A02	1	BD29202_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	AATTTAATTGTGACTAGGCATGGTCTG	138.47521
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.083	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29203_Cmae_Bot2011_COI	ADL-A01	1	BD29203_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	ATGAACAGTCTATCCTCCTTTAG	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29203_Hsan_Wil2021_COI	ADL-A01	1	BD29203_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	CCTGGGCCGGTATAGTAGGT	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29203_Mros_Gib2022_COI	ADL-A01	1	BD29203_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29203_Msal_Bot2012_COI	ADL-A01	1	BD29203_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	GYTAGTTCCRATGATGTTAGCTG	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29203_Mstr_Wil2021_COI	ADL-A01	1	BD29203_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	GTAGTGGTAACTACTCATGCTTTA	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29203_Pvir_Dia2013_COI	ADL-A01	1	BD29203_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	CTTAGTGGCATTAATTCGDAATCC	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29203_Rhar_Sim2018_COI	ADL-A01	1	BD29203_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	CTGCTGCTATTGCTCATG	138.47237
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29209_Aamu_Bax2006_COI	THE-A35	1	BD29209_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	GCACAACCGGGATCTTTACTTC	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29209_Pcan_Bot2011_IGS	THE-A35	1	BD29209_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A35	IGS	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	CGTAATCCTCAGTACTGGCTA	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29209_Pper_Dia2013_COI	THE-A35	1	BD29209_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	CTTAGTGGCATTAATTCGDAATCC	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29209_Sspa_Oph2007_COI	THE-A35	1	BD29209_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	GAAGGCCTTCCGATGATC	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29209_Upin_Bot2011_COI	THE-A35	1	BD29209_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	CTTTAATTACAGCGTTTTTATTGTTGT	133.64129
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29210_Cmae_Bot2011_COI	THE-A34	1	BD29210_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	ATGAACAGTCTATCCTCCTTTAG	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29210_Hsan_Wil2021_COI	THE-A34	1	BD29210_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	CCTGGGCCGGTATAGTAGGT	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29210_Mros_Gib2022_COI	THE-A34	1	BD29210_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29210_Msal_Bot2012_COI	THE-A34	1	BD29210_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	GYTAGTTCCRATGATGTTAGCTG	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29210_Mstr_Wil2021_COI	THE-A34	1	BD29210_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	GTAGTGGTAACTACTCATGCTTTA	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29210_Pvir_Dia2013_COI	THE-A34	1	BD29210_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	CTTAGTGGCATTAATTCGDAATCC	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29210_Rhar_Sim2018_COI	THE-A34	1	BD29210_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	CTGCTGCTATTGCTCATG	133.63997
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29212_Aamu_Bax2006_COI	THE-A32	1	BD29212_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	GCACAACCGGGATCTTTACTTC	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29212_Pcan_Bot2011_IGS	THE-A32	1	BD29212_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A32	IGS	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	CGTAATCCTCAGTACTGGCTA	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29212_Pper_Dia2013_COI	THE-A32	1	BD29212_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	CTTAGTGGCATTAATTCGDAATCC	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29212_Sspa_Oph2007_COI	THE-A32	1	BD29212_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	GAAGGCCTTCCGATGATC	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29212_Upin_Bot2011_COI	THE-A32	1	BD29212_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	CTTTAATTACAGCGTTTTTATTGTTGT	133.63713
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29213_Cjap_Sim2018_COI	THE-A31	1	BD29213_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29213_Esin_And2018_Cyb	THE-A31	1	BD29213_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A31	Cytb	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	ACCCCTCCTCATATCCAACCA	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29213_Mgig_Bot2012_COI	THE-A31	1	BD29213_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	TCTTATTCGTTGGAGACTTTATAACCCT	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29213_Mjap_Gib2022_COI	THE-A31	1	BD29213_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	AATTTAATTGTGACTAGGCATGGTCTG	133.63489
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29214_Cmae_Bot2011_COI	THE-A30	1	BD29214_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	ATGAACAGTCTATCCTCCTTTAG	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29214_Hsan_Wil2021_COI	THE-A30	1	BD29214_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	CCTGGGCCGGTATAGTAGGT	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29214_Mros_Gib2022_COI	THE-A30	1	BD29214_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29214_Msal_Bot2012_COI	THE-A30	1	BD29214_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	GYTAGTTCCRATGATGTTAGCTG	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29214_Mstr_Wil2021_COI	THE-A30	1	BD29214_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	GTAGTGGTAACTACTCATGCTTTA	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29214_Pvir_Dia2013_COI	THE-A30	1	BD29214_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	CTTAGTGGCATTAATTCGDAATCC	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29214_Rhar_Sim2018_COI	THE-A30	1	BD29214_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	CTGCTGCTATTGCTCATG	133.63639
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29216_Aamu_Bax2006_COI	THE-A28	1	BD29216_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	GCACAACCGGGATCTTTACTTC	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29216_Pcan_Bot2011_IGS	THE-A28	1	BD29216_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A28	IGS	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	CGTAATCCTCAGTACTGGCTA	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29216_Pper_Dia2013_COI	THE-A28	1	BD29216_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	CTTAGTGGCATTAATTCGDAATCC	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29216_Sspa_Oph2007_COI	THE-A28	1	BD29216_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	GAAGGCCTTCCGATGATC	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29216_Upin_Bot2011_COI	THE-A28	1	BD29216_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	CTTTAATTACAGCGTTTTTATTGTTGT	133.63866
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29217_Cjap_Sim2018_COI	THE-A27	1	BD29217_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29217_Esin_And2018_Cyb	THE-A27	1	BD29217_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A27	Cytb	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	ACCCCTCCTCATATCCAACCA	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29217_Mgig_Bot2012_COI	THE-A27	1	BD29217_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	TCTTATTCGTTGGAGACTTTATAACCCT	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29217_Mjap_Gib2022_COI	THE-A27	1	BD29217_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	AATTTAATTGTGACTAGGCATGGTCTG	133.64061
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29218_Cmae_Bot2011_COI	THE-A26	1	BD29218_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	ATGAACAGTCTATCCTCCTTTAG	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29218_Hsan_Wil2021_COI	THE-A26	1	BD29218_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	CCTGGGCCGGTATAGTAGGT	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29218_Mros_Gib2022_COI	THE-A26	1	BD29218_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29218_Msal_Bot2012_COI	THE-A26	1	BD29218_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	GYTAGTTCCRATGATGTTAGCTG	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29218_Mstr_Wil2021_COI	THE-A26	1	BD29218_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	GTAGTGGTAACTACTCATGCTTTA	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29218_Pvir_Dia2013_COI	THE-A26	1	BD29218_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	CTTAGTGGCATTAATTCGDAATCC	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29218_Rhar_Sim2018_COI	THE-A26	1	BD29218_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	CTGCTGCTATTGCTCATG	133.64141
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29220_Cjap_Sim2018_COI	THE-A24	1	BD29220_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29220_Esin_And2018_Cyb	THE-A24	1	BD29220_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A24	Cytb	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	ACCCCTCCTCATATCCAACCA	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29220_Mgig_Bot2012_COI	THE-A24	1	BD29220_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	TCTTATTCGTTGGAGACTTTATAACCCT	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29220_Mjap_Gib2022_COI	THE-A24	1	BD29220_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	AATTTAATTGTGACTAGGCATGGTCTG	133.63854
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29221_Cmae_Bot2011_COI	THE-A23	1	BD29221_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	ATGAACAGTCTATCCTCCTTTAG	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29221_Hsan_Wil2021_COI	THE-A23	1	BD29221_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	CCTGGGCCGGTATAGTAGGT	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29221_Mros_Gib2022_COI	THE-A23	1	BD29221_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29221_Msal_Bot2012_COI	THE-A23	1	BD29221_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	GYTAGTTCCRATGATGTTAGCTG	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29221_Mstr_Wil2021_COI	THE-A23	1	BD29221_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	GTAGTGGTAACTACTCATGCTTTA	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29221_Pvir_Dia2013_COI	THE-A23	1	BD29221_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	CTTAGTGGCATTAATTCGDAATCC	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29221_Rhar_Sim2018_COI	THE-A23	1	BD29221_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	CTGCTGCTATTGCTCATG	133.63701
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29223_Aamu_Bax2006_COI	THE-A21	1	BD29223_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	GCACAACCGGGATCTTTACTTC	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29223_Pcan_Bot2011_IGS	THE-A21	1	BD29223_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A21	IGS	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	CGTAATCCTCAGTACTGGCTA	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29223_Pper_Dia2013_COI	THE-A21	1	BD29223_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	CTTAGTGGCATTAATTCGDAATCC	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29223_Sspa_Oph2007_COI	THE-A21	1	BD29223_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	GAAGGCCTTCCGATGATC	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29223_Upin_Bot2011_COI	THE-A21	1	BD29223_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	CTTTAATTACAGCGTTTTTATTGTTGT	133.63495
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29224_Cjap_Sim2018_COI	THE-A20	1	BD29224_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29224_Esin_And2018_Cyb	THE-A20	1	BD29224_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A20	Cytb	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	ACCCCTCCTCATATCCAACCA	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29224_Mgig_Bot2012_COI	THE-A20	1	BD29224_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	TCTTATTCGTTGGAGACTTTATAACCCT	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29224_Mjap_Gib2022_COI	THE-A20	1	BD29224_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	AATTTAATTGTGACTAGGCATGGTCTG	133.63631
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29225_Cmae_Bot2011_COI	THE-A19	1	BD29225_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	ATGAACAGTCTATCCTCCTTTAG	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29225_Hsan_Wil2021_COI	THE-A19	1	BD29225_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	CCTGGGCCGGTATAGTAGGT	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29225_Mros_Gib2022_COI	THE-A19	1	BD29225_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29225_Msal_Bot2012_COI	THE-A19	1	BD29225_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	GYTAGTTCCRATGATGTTAGCTG	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29225_Mstr_Wil2021_COI	THE-A19	1	BD29225_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	GTAGTGGTAACTACTCATGCTTTA	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29225_Pvir_Dia2013_COI	THE-A19	1	BD29225_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	CTTAGTGGCATTAATTCGDAATCC	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29225_Rhar_Sim2018_COI	THE-A19	1	BD29225_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	CTGCTGCTATTGCTCATG	133.63764
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29227_Aamu_Bax2006_COI	THE-A17	1	BD29227_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	GCACAACCGGGATCTTTACTTC	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29227_Pcan_Bot2011_IGS	THE-A17	1	BD29227_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A17	IGS	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	CGTAATCCTCAGTACTGGCTA	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29227_Pper_Dia2013_COI	THE-A17	1	BD29227_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	CTTAGTGGCATTAATTCGDAATCC	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29227_Sspa_Oph2007_COI	THE-A17	1	BD29227_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	GAAGGCCTTCCGATGATC	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29227_Upin_Bot2011_COI	THE-A17	1	BD29227_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	CTTTAATTACAGCGTTTTTATTGTTGT	133.63938
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29228_Cjap_Sim2018_COI	THE-A16	1	BD29228_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29228_Esin_And2018_Cyb	THE-A16	1	BD29228_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A16	Cytb	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	ACCCCTCCTCATATCCAACCA	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29228_Mgig_Bot2012_COI	THE-A16	1	BD29228_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	TCTTATTCGTTGGAGACTTTATAACCCT	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29228_Mjap_Gib2022_COI	THE-A16	1	BD29228_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	AATTTAATTGTGACTAGGCATGGTCTG	133.64079
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29229_Cmae_Bot2011_COI	THE-A15	1	BD29229_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	ATGAACAGTCTATCCTCCTTTAG	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29229_Hsan_Wil2021_COI	THE-A15	1	BD29229_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	CCTGGGCCGGTATAGTAGGT	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29229_Mros_Gib2022_COI	THE-A15	1	BD29229_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29229_Msal_Bot2012_COI	THE-A15	1	BD29229_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	GYTAGTTCCRATGATGTTAGCTG	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29229_Mstr_Wil2021_COI	THE-A15	1	BD29229_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	GTAGTGGTAACTACTCATGCTTTA	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29229_Pvir_Dia2013_COI	THE-A15	1	BD29229_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	CTTAGTGGCATTAATTCGDAATCC	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29229_Rhar_Sim2018_COI	THE-A15	1	BD29229_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	CTGCTGCTATTGCTCATG	133.63994
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29230_Aamu_Bax2006_COI	THE-A14	1	BD29230_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	GCACAACCGGGATCTTTACTTC	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29230_Pcan_Bot2011_IGS	THE-A14	1	BD29230_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A14	IGS	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	CGTAATCCTCAGTACTGGCTA	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29230_Pper_Dia2013_COI	THE-A14	1	BD29230_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	CTTAGTGGCATTAATTCGDAATCC	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29230_Sspa_Oph2007_COI	THE-A14	1	BD29230_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	GAAGGCCTTCCGATGATC	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29230_Upin_Bot2011_COI	THE-A14	1	BD29230_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	CTTTAATTACAGCGTTTTTATTGTTGT	133.63852
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29231_Cjap_Sim2018_COI	THE-A13	1	BD29231_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29231_Esin_And2018_Cyb	THE-A13	1	BD29231_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A13	Cytb	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	ACCCCTCCTCATATCCAACCA	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29231_Mgig_Bot2012_COI	THE-A13	1	BD29231_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	TCTTATTCGTTGGAGACTTTATAACCCT	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29231_Mjap_Gib2022_COI	THE-A13	1	BD29231_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	AATTTAATTGTGACTAGGCATGGTCTG	133.63711
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29232_Cmae_Bot2011_COI	THE-A11	1	BD29232_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	ATGAACAGTCTATCCTCCTTTAG	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29232_Hsan_Wil2021_COI	THE-A11	1	BD29232_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	CCTGGGCCGGTATAGTAGGT	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29232_Mros_Gib2022_COI	THE-A11	1	BD29232_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29232_Msal_Bot2012_COI	THE-A11	1	BD29232_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	GYTAGTTCCRATGATGTTAGCTG	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29232_Mstr_Wil2021_COI	THE-A11	1	BD29232_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	GTAGTGGTAACTACTCATGCTTTA	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29232_Pvir_Dia2013_COI	THE-A11	1	BD29232_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	CTTAGTGGCATTAATTCGDAATCC	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29232_Rhar_Sim2018_COI	THE-A11	1	BD29232_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	CTGCTGCTATTGCTCATG	133.6349
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29234_Aamu_Bax2006_COI	THE-A10	1	BD29234_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	GCACAACCGGGATCTTTACTTC	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29234_Pcan_Bot2011_IGS	THE-A10	1	BD29234_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A10	IGS	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	CGTAATCCTCAGTACTGGCTA	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29234_Pper_Dia2013_COI	THE-A10	1	BD29234_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	CTTAGTGGCATTAATTCGDAATCC	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29234_Sspa_Oph2007_COI	THE-A10	1	BD29234_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	GAAGGCCTTCCGATGATC	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29234_Upin_Bot2011_COI	THE-A10	1	BD29234_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	CTTTAATTACAGCGTTTTTATTGTTGT	133.63622
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29235_Cjap_Sim2018_COI	THE-A09	1	BD29235_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29235_Esin_And2018_Cyb	THE-A09	1	BD29235_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A09	Cytb	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	ACCCCTCCTCATATCCAACCA	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29235_Mgig_Bot2012_COI	THE-A09	1	BD29235_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	TCTTATTCGTTGGAGACTTTATAACCCT	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29235_Mjap_Gib2022_COI	THE-A09	1	BD29235_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	AATTTAATTGTGACTAGGCATGGTCTG	133.63786
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29236_Cmae_Bot2011_COI	THE-A08	1	BD29236_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	ATGAACAGTCTATCCTCCTTTAG	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29236_Hsan_Wil2021_COI	THE-A08	1	BD29236_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	CCTGGGCCGGTATAGTAGGT	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29236_Mros_Gib2022_COI	THE-A08	1	BD29236_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29236_Msal_Bot2012_COI	THE-A08	1	BD29236_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	GYTAGTTCCRATGATGTTAGCTG	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29236_Mstr_Wil2021_COI	THE-A08	1	BD29236_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	GTAGTGGTAACTACTCATGCTTTA	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29236_Pvir_Dia2013_COI	THE-A08	1	BD29236_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	CTTAGTGGCATTAATTCGDAATCC	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29236_Rhar_Sim2018_COI	THE-A08	1	BD29236_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	CTGCTGCTATTGCTCATG	133.63907
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29238_Aamu_Bax2006_COI	THE-A06	1	BD29238_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	GCACAACCGGGATCTTTACTTC	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29238_Pcan_Bot2011_IGS	THE-A06	1	BD29238_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A06	IGS	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	CGTAATCCTCAGTACTGGCTA	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29238_Pper_Dia2013_COI	THE-A06	1	BD29238_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	CTTAGTGGCATTAATTCGDAATCC	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29238_Sspa_Oph2007_COI	THE-A06	1	BD29238_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	GAAGGCCTTCCGATGATC	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29238_Upin_Bot2011_COI	THE-A06	1	BD29238_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	CTTTAATTACAGCGTTTTTATTGTTGT	133.64205
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29239_Cjap_Sim2018_COI	THE-A05	1	BD29239_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29239_Esin_And2018_Cyb	THE-A05	1	BD29239_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A05	Cytb	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	ACCCCTCCTCATATCCAACCA	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.172	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29239_Mgig_Bot2012_COI	THE-A05	1	BD29239_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Present	Sample	Mgig_Bot2012_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	TCTTATTCGTTGGAGACTTTATAACCCT	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29239_Mjap_Gib2022_COI	THE-A05	1	BD29239_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	AATTTAATTGTGACTAGGCATGGTCTG	133.64142
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29241_Aamu_Bax2006_COI	THE-A03	1	BD29241_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	GCACAACCGGGATCTTTACTTC	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29241_Pcan_Bot2011_IGS	THE-A03	1	BD29241_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A03	IGS	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	CGTAATCCTCAGTACTGGCTA	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29241_Pper_Dia2013_COI	THE-A03	1	BD29241_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	CTTAGTGGCATTAATTCGDAATCC	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29241_Sspa_Oph2007_COI	THE-A03	1	BD29241_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	GAAGGCCTTCCGATGATC	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29241_Upin_Bot2011_COI	THE-A03	1	BD29241_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	CTTTAATTACAGCGTTTTTATTGTTGT	133.63825
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29242_Cjap_Sim2018_COI	THE-A02	1	BD29242_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29242_Esin_And2018_Cyb	THE-A02	1	BD29242_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A02	Cytb	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	ACCCCTCCTCATATCCAACCA	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29242_Mgig_Bot2012_COI	THE-A02	1	BD29242_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	TCTTATTCGTTGGAGACTTTATAACCCT	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29242_Mjap_Gib2022_COI	THE-A02	1	BD29242_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	AATTTAATTGTGACTAGGCATGGTCTG	133.6372
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29243_Cmae_Bot2011_COI	THE-A01	1	BD29243_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	ATGAACAGTCTATCCTCCTTTAG	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29243_Hsan_Wil2021_COI	THE-A01	1	BD29243_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	CCTGGGCCGGTATAGTAGGT	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29243_Mros_Gib2022_COI	THE-A01	1	BD29243_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29243_Msal_Bot2012_COI	THE-A01	1	BD29243_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	GYTAGTTCCRATGATGTTAGCTG	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29243_Mstr_Wil2021_COI	THE-A01	1	BD29243_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	GTAGTGGTAACTACTCATGCTTTA	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29243_Pvir_Dia2013_COI	THE-A01	1	BD29243_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	CTTAGTGGCATTAATTCGDAATCC	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29243_Rhar_Sim2018_COI	THE-A01	1	BD29243_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	CTGCTGCTATTGCTCATG	133.63322
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29245_Aamu_Bax2006_COI	PLO-A39	1	BD29245_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	GCACAACCGGGATCTTTACTTC	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29245_Pcan_Bot2011_IGS	PLO-A39	1	BD29245_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A39	IGS	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	CGTAATCCTCAGTACTGGCTA	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29245_Pper_Dia2013_COI	PLO-A39	1	BD29245_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	CTTAGTGGCATTAATTCGDAATCC	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.132	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29245_Sspa_Oph2007_COI	PLO-A39	1	BD29245_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	GAAGGCCTTCCGATGATC	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29245_Upin_Bot2011_COI	PLO-A39	1	BD29245_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	CTTTAATTACAGCGTTTTTATTGTTGT	135.8719
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29246_Cjap_Sim2018_COI	PLO-A38	1	BD29246_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29246_Esin_And2018_Cyb	PLO-A38	1	BD29246_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A38	Cytb	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	ACCCCTCCTCATATCCAACCA	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29246_Mgig_Bot2012_COI	PLO-A38	1	BD29246_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	TCTTATTCGTTGGAGACTTTATAACCCT	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29246_Mjap_Gib2022_COI	PLO-A38	1	BD29246_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	AATTTAATTGTGACTAGGCATGGTCTG	135.87366
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29247_Cmae_Bot2011_COI	PLO-A37	1	BD29247_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	ATGAACAGTCTATCCTCCTTTAG	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29247_Hsan_Wil2021_COI	PLO-A37	1	BD29247_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	CCTGGGCCGGTATAGTAGGT	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29247_Mros_Gib2022_COI	PLO-A37	1	BD29247_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29247_Msal_Bot2012_COI	PLO-A37	1	BD29247_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	GYTAGTTCCRATGATGTTAGCTG	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29247_Mstr_Wil2021_COI	PLO-A37	1	BD29247_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	GTAGTGGTAACTACTCATGCTTTA	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29247_Pvir_Dia2013_COI	PLO-A37	1	BD29247_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	CTTAGTGGCATTAATTCGDAATCC	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29247_Rhar_Sim2018_COI	PLO-A37	1	BD29247_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	CTGCTGCTATTGCTCATG	135.87481
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29249_Aamu_Bax2006_COI	PLO-A35	1	BD29249_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	GCACAACCGGGATCTTTACTTC	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29249_Pcan_Bot2011_IGS	PLO-A35	1	BD29249_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A35	IGS	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	CGTAATCCTCAGTACTGGCTA	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29249_Pper_Dia2013_COI	PLO-A35	1	BD29249_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	CTTAGTGGCATTAATTCGDAATCC	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.359	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29249_Sspa_Oph2007_COI	PLO-A35	1	BD29249_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	GAAGGCCTTCCGATGATC	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29249_Upin_Bot2011_COI	PLO-A35	1	BD29249_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	CTTTAATTACAGCGTTTTTATTGTTGT	135.87281
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29250_Cmae_Bot2011_COI	PLO-A34	1	BD29250_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	ATGAACAGTCTATCCTCCTTTAG	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29250_Hsan_Wil2021_COI	PLO-A34	1	BD29250_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	CCTGGGCCGGTATAGTAGGT	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29250_Mros_Gib2022_COI	PLO-A34	1	BD29250_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29250_Msal_Bot2012_COI	PLO-A34	1	BD29250_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	GYTAGTTCCRATGATGTTAGCTG	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29250_Mstr_Wil2021_COI	PLO-A34	1	BD29250_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	GTAGTGGTAACTACTCATGCTTTA	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29250_Pvir_Dia2013_COI	PLO-A34	1	BD29250_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	CTTAGTGGCATTAATTCGDAATCC	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29250_Rhar_Sim2018_COI	PLO-A34	1	BD29250_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	CTGCTGCTATTGCTCATG	135.8746
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29252_Aamu_Bax2006_COI	PLO-A32	1	BD29252_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	GCACAACCGGGATCTTTACTTC	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29252_Pcan_Bot2011_IGS	PLO-A32	1	BD29252_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A32	IGS	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	CGTAATCCTCAGTACTGGCTA	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29252_Pper_Dia2013_COI	PLO-A32	1	BD29252_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	CTTAGTGGCATTAATTCGDAATCC	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.292	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29252_Sspa_Oph2007_COI	PLO-A32	1	BD29252_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	GAAGGCCTTCCGATGATC	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29252_Upin_Bot2011_COI	PLO-A32	1	BD29252_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	CTTTAATTACAGCGTTTTTATTGTTGT	135.87354
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29253_Cjap_Sim2018_COI	PLO-A31	1	BD29253_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29253_Esin_And2018_Cyb	PLO-A31	1	BD29253_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A31	Cytb	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	ACCCCTCCTCATATCCAACCA	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29253_Mgig_Bot2012_COI	PLO-A31	1	BD29253_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	TCTTATTCGTTGGAGACTTTATAACCCT	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29253_Mjap_Gib2022_COI	PLO-A31	1	BD29253_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	AATTTAATTGTGACTAGGCATGGTCTG	135.87589
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29254_Cmae_Bot2011_COI	PLO-A30	1	BD29254_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	ATGAACAGTCTATCCTCCTTTAG	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29254_Hsan_Wil2021_COI	PLO-A30	1	BD29254_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	CCTGGGCCGGTATAGTAGGT	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29254_Mros_Gib2022_COI	PLO-A30	1	BD29254_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29254_Msal_Bot2012_COI	PLO-A30	1	BD29254_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	GYTAGTTCCRATGATGTTAGCTG	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29254_Mstr_Wil2021_COI	PLO-A30	1	BD29254_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	GTAGTGGTAACTACTCATGCTTTA	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29254_Pvir_Dia2013_COI	PLO-A30	1	BD29254_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	CTTAGTGGCATTAATTCGDAATCC	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29254_Rhar_Sim2018_COI	PLO-A30	1	BD29254_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	CTGCTGCTATTGCTCATG	135.87692
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29256_Aamu_Bax2006_COI	PLO-A28	1	BD29256_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	GCACAACCGGGATCTTTACTTC	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29256_Pcan_Bot2011_IGS	PLO-A28	1	BD29256_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A28	IGS	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	CGTAATCCTCAGTACTGGCTA	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29256_Pper_Dia2013_COI	PLO-A28	1	BD29256_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	CTTAGTGGCATTAATTCGDAATCC	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29256_Sspa_Oph2007_COI	PLO-A28	1	BD29256_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	GAAGGCCTTCCGATGATC	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29256_Upin_Bot2011_COI	PLO-A28	1	BD29256_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	CTTTAATTACAGCGTTTTTATTGTTGT	135.87249
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29257_Cjap_Sim2018_COI	PLO-A27	1	BD29257_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29257_Esin_And2018_Cyb	PLO-A27	1	BD29257_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A27	Cytb	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	ACCCCTCCTCATATCCAACCA	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29257_Mgig_Bot2012_COI	PLO-A27	1	BD29257_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	TCTTATTCGTTGGAGACTTTATAACCCT	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29257_Mjap_Gib2022_COI	PLO-A27	1	BD29257_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	AATTTAATTGTGACTAGGCATGGTCTG	135.87467
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29258_Cmae_Bot2011_COI	PLO-A26	1	BD29258_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	ATGAACAGTCTATCCTCCTTTAG	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29258_Hsan_Wil2021_COI	PLO-A26	1	BD29258_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	CCTGGGCCGGTATAGTAGGT	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29258_Mros_Gib2022_COI	PLO-A26	1	BD29258_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29258_Msal_Bot2012_COI	PLO-A26	1	BD29258_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	GYTAGTTCCRATGATGTTAGCTG	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29258_Mstr_Wil2021_COI	PLO-A26	1	BD29258_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	GTAGTGGTAACTACTCATGCTTTA	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29258_Pvir_Dia2013_COI	PLO-A26	1	BD29258_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	CTTAGTGGCATTAATTCGDAATCC	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29258_Rhar_Sim2018_COI	PLO-A26	1	BD29258_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	CTGCTGCTATTGCTCATG	135.87367
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29260_Cjap_Sim2018_COI	PLO-A22	1	BD29260_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29260_Esin_And2018_Cyb	PLO-A22	1	BD29260_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A22	Cytb	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	ACCCCTCCTCATATCCAACCA	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29260_Mgig_Bot2012_COI	PLO-A22	1	BD29260_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	TCTTATTCGTTGGAGACTTTATAACCCT	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29260_Mjap_Gib2022_COI	PLO-A22	1	BD29260_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	AATTTAATTGTGACTAGGCATGGTCTG	135.86801
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29261_Cmae_Bot2011_COI	PLO-A24	1	BD29261_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	ATGAACAGTCTATCCTCCTTTAG	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29261_Hsan_Wil2021_COI	PLO-A24	1	BD29261_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	CCTGGGCCGGTATAGTAGGT	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29261_Mros_Gib2022_COI	PLO-A24	1	BD29261_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29261_Msal_Bot2012_COI	PLO-A24	1	BD29261_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	GYTAGTTCCRATGATGTTAGCTG	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29261_Mstr_Wil2021_COI	PLO-A24	1	BD29261_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	GTAGTGGTAACTACTCATGCTTTA	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29261_Pvir_Dia2013_COI	PLO-A24	1	BD29261_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	CTTAGTGGCATTAATTCGDAATCC	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29261_Rhar_Sim2018_COI	PLO-A24	1	BD29261_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	CTGCTGCTATTGCTCATG	135.87139
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29264_Cjap_Sim2018_COI	PLO-A21	1	BD29264_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29264_Esin_And2018_Cyb	PLO-A21	1	BD29264_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A21	Cytb	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	ACCCCTCCTCATATCCAACCA	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29264_Mgig_Bot2012_COI	PLO-A21	1	BD29264_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	TCTTATTCGTTGGAGACTTTATAACCCT	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29264_Mjap_Gib2022_COI	PLO-A21	1	BD29264_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	AATTTAATTGTGACTAGGCATGGTCTG	135.86904
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29265_Cmae_Bot2011_COI	PLO-A20	1	BD29265_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	ATGAACAGTCTATCCTCCTTTAG	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29265_Hsan_Wil2021_COI	PLO-A20	1	BD29265_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	CCTGGGCCGGTATAGTAGGT	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29265_Mros_Gib2022_COI	PLO-A20	1	BD29265_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29265_Msal_Bot2012_COI	PLO-A20	1	BD29265_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	GYTAGTTCCRATGATGTTAGCTG	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29265_Mstr_Wil2021_COI	PLO-A20	1	BD29265_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	GTAGTGGTAACTACTCATGCTTTA	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29265_Pvir_Dia2013_COI	PLO-A20	1	BD29265_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	CTTAGTGGCATTAATTCGDAATCC	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29265_Rhar_Sim2018_COI	PLO-A20	1	BD29265_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	CTGCTGCTATTGCTCATG	135.86686
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29267_Aamu_Bax2006_COI	PLO-A18	1	BD29267_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	GCACAACCGGGATCTTTACTTC	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29267_Pcan_Bot2011_IGS	PLO-A18	1	BD29267_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A18	IGS	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	CGTAATCCTCAGTACTGGCTA	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29267_Pper_Dia2013_COI	PLO-A18	1	BD29267_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	CTTAGTGGCATTAATTCGDAATCC	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.931	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29267_Sspa_Oph2007_COI	PLO-A18	1	BD29267_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	GAAGGCCTTCCGATGATC	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29267_Upin_Bot2011_COI	PLO-A18	1	BD29267_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	CTTTAATTACAGCGTTTTTATTGTTGT	135.86819
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29268_Cjap_Sim2018_COI	PLO-A17	1	BD29268_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29268_Esin_And2018_Cyb	PLO-A17	1	BD29268_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A17	Cytb	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	ACCCCTCCTCATATCCAACCA	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29268_Mgig_Bot2012_COI	PLO-A17	1	BD29268_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	TCTTATTCGTTGGAGACTTTATAACCCT	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29268_Mjap_Gib2022_COI	PLO-A17	1	BD29268_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	AATTTAATTGTGACTAGGCATGGTCTG	135.8648
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29269_Cmae_Bot2011_COI	PLO-A16	1	BD29269_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	ATGAACAGTCTATCCTCCTTTAG	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29269_Hsan_Wil2021_COI	PLO-A16	1	BD29269_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	CCTGGGCCGGTATAGTAGGT	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29269_Mros_Gib2022_COI	PLO-A16	1	BD29269_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29269_Msal_Bot2012_COI	PLO-A16	1	BD29269_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	GYTAGTTCCRATGATGTTAGCTG	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29269_Mstr_Wil2021_COI	PLO-A16	1	BD29269_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	GTAGTGGTAACTACTCATGCTTTA	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29269_Pvir_Dia2013_COI	PLO-A16	1	BD29269_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	CTTAGTGGCATTAATTCGDAATCC	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29269_Rhar_Sim2018_COI	PLO-A16	1	BD29269_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	CTGCTGCTATTGCTCATG	135.86685
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29270_Aamu_Bax2006_COI	PLO-A15	1	BD29270_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	GCACAACCGGGATCTTTACTTC	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29270_Pcan_Bot2011_IGS	PLO-A15	1	BD29270_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A15	IGS	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	CGTAATCCTCAGTACTGGCTA	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29270_Pper_Dia2013_COI	PLO-A15	1	BD29270_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	CTTAGTGGCATTAATTCGDAATCC	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.82	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29270_Sspa_Oph2007_COI	PLO-A15	1	BD29270_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	GAAGGCCTTCCGATGATC	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29270_Upin_Bot2011_COI	PLO-A15	1	BD29270_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	CTTTAATTACAGCGTTTTTATTGTTGT	135.86365
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29271_Cjap_Sim2018_COI	PLO-A14	1	BD29271_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29271_Esin_And2018_Cyb	PLO-A14	1	BD29271_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A14	Cytb	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	ACCCCTCCTCATATCCAACCA	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29271_Mgig_Bot2012_COI	PLO-A14	1	BD29271_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	TCTTATTCGTTGGAGACTTTATAACCCT	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29271_Mjap_Gib2022_COI	PLO-A14	1	BD29271_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	AATTTAATTGTGACTAGGCATGGTCTG	135.86587
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29272_Cmae_Bot2011_COI	PLO-A13	1	BD29272_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	ATGAACAGTCTATCCTCCTTTAG	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29272_Hsan_Wil2021_COI	PLO-A13	1	BD29272_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	CCTGGGCCGGTATAGTAGGT	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29272_Mros_Gib2022_COI	PLO-A13	1	BD29272_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29272_Msal_Bot2012_COI	PLO-A13	1	BD29272_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	GYTAGTTCCRATGATGTTAGCTG	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29272_Mstr_Wil2021_COI	PLO-A13	1	BD29272_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	GTAGTGGTAACTACTCATGCTTTA	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29272_Pvir_Dia2013_COI	PLO-A13	1	BD29272_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	CTTAGTGGCATTAATTCGDAATCC	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29272_Rhar_Sim2018_COI	PLO-A13	1	BD29272_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	CTGCTGCTATTGCTCATG	135.86812
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29274_Aamu_Bax2006_COI	PLO-A11	1	BD29274_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	GCACAACCGGGATCTTTACTTC	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29274_Pcan_Bot2011_IGS	PLO-A11	1	BD29274_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A11	IGS	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	CGTAATCCTCAGTACTGGCTA	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29274_Pper_Dia2013_COI	PLO-A11	1	BD29274_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	CTTAGTGGCATTAATTCGDAATCC	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.52	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29274_Sspa_Oph2007_COI	PLO-A11	1	BD29274_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	GAAGGCCTTCCGATGATC	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29274_Upin_Bot2011_COI	PLO-A11	1	BD29274_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	CTTTAATTACAGCGTTTTTATTGTTGT	135.86498
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29275_Cjap_Sim2018_COI	PLO-A10	1	BD29275_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29275_Esin_And2018_Cyb	PLO-A10	1	BD29275_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A10	Cytb	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	ACCCCTCCTCATATCCAACCA	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29275_Mgig_Bot2012_COI	PLO-A10	1	BD29275_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	TCTTATTCGTTGGAGACTTTATAACCCT	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29275_Mjap_Gib2022_COI	PLO-A10	1	BD29275_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	AATTTAATTGTGACTAGGCATGGTCTG	135.8636
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29276_Cmae_Bot2011_COI	PLO-A09	1	BD29276_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	ATGAACAGTCTATCCTCCTTTAG	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29276_Hsan_Wil2021_COI	PLO-A09	1	BD29276_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	CCTGGGCCGGTATAGTAGGT	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29276_Mros_Gib2022_COI	PLO-A09	1	BD29276_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29276_Msal_Bot2012_COI	PLO-A09	1	BD29276_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	GYTAGTTCCRATGATGTTAGCTG	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29276_Mstr_Wil2021_COI	PLO-A09	1	BD29276_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	GTAGTGGTAACTACTCATGCTTTA	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29276_Pvir_Dia2013_COI	PLO-A09	1	BD29276_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	CTTAGTGGCATTAATTCGDAATCC	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29276_Rhar_Sim2018_COI	PLO-A09	1	BD29276_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	CTGCTGCTATTGCTCATG	135.87123
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29278_Aamu_Bax2006_COI	PLO-A07	1	BD29278_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	GCACAACCGGGATCTTTACTTC	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29278_Pcan_Bot2011_IGS	PLO-A07	1	BD29278_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A07	IGS	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	CGTAATCCTCAGTACTGGCTA	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29278_Pper_Dia2013_COI	PLO-A07	1	BD29278_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	CTTAGTGGCATTAATTCGDAATCC	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.179	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29278_Sspa_Oph2007_COI	PLO-A07	1	BD29278_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	GAAGGCCTTCCGATGATC	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29278_Upin_Bot2011_COI	PLO-A07	1	BD29278_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	CTTTAATTACAGCGTTTTTATTGTTGT	135.8729
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29279_Cjap_Sim2018_COI	PLO-A06	1	BD29279_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29279_Esin_And2018_Cyb	PLO-A06	1	BD29279_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A06	Cytb	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	ACCCCTCCTCATATCCAACCA	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29279_Mgig_Bot2012_COI	PLO-A06	1	BD29279_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	TCTTATTCGTTGGAGACTTTATAACCCT	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29279_Mjap_Gib2022_COI	PLO-A06	1	BD29279_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	AATTTAATTGTGACTAGGCATGGTCTG	135.8739
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29281_Aamu_Bax2006_COI	PLO-A04	1	BD29281_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	GCACAACCGGGATCTTTACTTC	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29281_Pcan_Bot2011_IGS	PLO-A04	1	BD29281_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A04	IGS	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	CGTAATCCTCAGTACTGGCTA	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29281_Pper_Dia2013_COI	PLO-A04	1	BD29281_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	CTTAGTGGCATTAATTCGDAATCC	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.84	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29281_Sspa_Oph2007_COI	PLO-A04	1	BD29281_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	GAAGGCCTTCCGATGATC	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29281_Upin_Bot2011_COI	PLO-A04	1	BD29281_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	CTTTAATTACAGCGTTTTTATTGTTGT	135.87711
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29282_Cjap_Sim2018_COI	PLO-A03	1	BD29282_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29282_Esin_And2018_Cyb	PLO-A03	1	BD29282_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A03	Cytb	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	ACCCCTCCTCATATCCAACCA	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29282_Mgig_Bot2012_COI	PLO-A03	1	BD29282_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	TCTTATTCGTTGGAGACTTTATAACCCT	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29282_Mjap_Gib2022_COI	PLO-A03	1	BD29282_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	AATTTAATTGTGACTAGGCATGGTCTG	135.87891
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29283_Cmae_Bot2011_COI	PLO-A02	1	BD29283_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	ATGAACAGTCTATCCTCCTTTAG	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29283_Hsan_Wil2021_COI	PLO-A02	1	BD29283_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	CCTGGGCCGGTATAGTAGGT	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29283_Mros_Gib2022_COI	PLO-A02	1	BD29283_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29283_Msal_Bot2012_COI	PLO-A02	1	BD29283_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	GYTAGTTCCRATGATGTTAGCTG	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29283_Mstr_Wil2021_COI	PLO-A02	1	BD29283_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	GTAGTGGTAACTACTCATGCTTTA	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29283_Pvir_Dia2013_COI	PLO-A02	1	BD29283_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	CTTAGTGGCATTAATTCGDAATCC	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29283_Rhar_Sim2018_COI	PLO-A02	1	BD29283_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	CTGCTGCTATTGCTCATG	135.87965
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29285_Aamu_Bax2006_COI	POR-A35	1	BD29285_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	GCACAACCGGGATCTTTACTTC	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29285_Pcan_Bot2011_IGS	POR-A35	1	BD29285_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A35	IGS	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	CGTAATCCTCAGTACTGGCTA	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29285_Pper_Dia2013_COI	POR-A35	1	BD29285_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	CTTAGTGGCATTAATTCGDAATCC	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.401	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29285_Sspa_Oph2007_COI	POR-A35	1	BD29285_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	GAAGGCCTTCCGATGATC	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.78	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29285_Upin_Bot2011_COI	POR-A35	1	BD29285_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Present	Sample	Upin_Bot2011_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	CTTTAATTACAGCGTTTTTATTGTTGT	141.61391
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29286_Cjap_Sim2018_COI	POR-A34	1	BD29286_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29286_Esin_And2018_Cyb	POR-A34	1	BD29286_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A34	Cytb	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	ACCCCTCCTCATATCCAACCA	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29286_Mgig_Bot2012_COI	POR-A34	1	BD29286_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	TCTTATTCGTTGGAGACTTTATAACCCT	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29286_Mjap_Gib2022_COI	POR-A34	1	BD29286_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	AATTTAATTGTGACTAGGCATGGTCTG	141.61249
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29287_Cmae_Bot2011_COI	POR-A33	1	BD29287_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	ATGAACAGTCTATCCTCCTTTAG	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29287_Hsan_Wil2021_COI	POR-A33	1	BD29287_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	CCTGGGCCGGTATAGTAGGT	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29287_Mros_Gib2022_COI	POR-A33	1	BD29287_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29287_Msal_Bot2012_COI	POR-A33	1	BD29287_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	GYTAGTTCCRATGATGTTAGCTG	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29287_Mstr_Wil2021_COI	POR-A33	1	BD29287_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	GTAGTGGTAACTACTCATGCTTTA	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29287_Pvir_Dia2013_COI	POR-A33	1	BD29287_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	CTTAGTGGCATTAATTCGDAATCC	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29287_Rhar_Sim2018_COI	POR-A33	1	BD29287_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	CTGCTGCTATTGCTCATG	141.61111
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29289_Aamu_Bax2006_COI	POR-A31	1	BD29289_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	GCACAACCGGGATCTTTACTTC	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29289_Pcan_Bot2011_IGS	POR-A31	1	BD29289_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A31	IGS	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	CGTAATCCTCAGTACTGGCTA	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29289_Pper_Dia2013_COI	POR-A31	1	BD29289_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	CTTAGTGGCATTAATTCGDAATCC	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29289_Sspa_Oph2007_COI	POR-A31	1	BD29289_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	GAAGGCCTTCCGATGATC	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.785	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29289_Upin_Bot2011_COI	POR-A31	1	BD29289_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Present	Sample	Upin_Bot2011_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	CTTTAATTACAGCGTTTTTATTGTTGT	141.62149
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29290_Cmae_Bot2011_COI	POR-A30	1	BD29290_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	ATGAACAGTCTATCCTCCTTTAG	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29290_Hsan_Wil2021_COI	POR-A30	1	BD29290_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	CCTGGGCCGGTATAGTAGGT	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.658	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29290_Mros_Gib2022_COI	POR-A30	1	BD29290_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Present	Sample	Mros_Gib2022_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29290_Msal_Bot2012_COI	POR-A30	1	BD29290_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	GYTAGTTCCRATGATGTTAGCTG	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29290_Mstr_Wil2021_COI	POR-A30	1	BD29290_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	GTAGTGGTAACTACTCATGCTTTA	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29290_Pvir_Dia2013_COI	POR-A30	1	BD29290_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	CTTAGTGGCATTAATTCGDAATCC	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29290_Rhar_Sim2018_COI	POR-A30	1	BD29290_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	CTGCTGCTATTGCTCATG	141.61982
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29292_Aamu_Bax2006_COI	POR-A28	1	BD29292_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	GCACAACCGGGATCTTTACTTC	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29292_Pcan_Bot2011_IGS	POR-A28	1	BD29292_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A28	IGS	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	CGTAATCCTCAGTACTGGCTA	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29292_Pper_Dia2013_COI	POR-A28	1	BD29292_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	CTTAGTGGCATTAATTCGDAATCC	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.593	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29292_Sspa_Oph2007_COI	POR-A28	1	BD29292_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	GAAGGCCTTCCGATGATC	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.629	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29292_Upin_Bot2011_COI	POR-A28	1	BD29292_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Present	Sample	Upin_Bot2011_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	CTTTAATTACAGCGTTTTTATTGTTGT	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29292_Vgib_Wil2025_COI	POR-A28	1	BD29292_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	CCCCTCTTTACAAGTTCTTTAGGGT	141.61735
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29293_Asen_Wil2025_COI	POR-A27	1	BD29293_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	GTTGTTGTTACAACACATGCTCTTTTG	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29293_Cjap_Sim2018_COI	POR-A27	1	BD29293_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29293_Esin_And2018_Cyb	POR-A27	1	BD29293_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A27	Cytb	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	ACCCCTCCTCATATCCAACCA	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29293_Mgig_Bot2012_COI	POR-A27	1	BD29293_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	TCTTATTCGTTGGAGACTTTATAACCCT	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29293_Mjap_Gib2022_COI	POR-A27	1	BD29293_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	AATTTAATTGTGACTAGGCATGGTCTG	141.61596
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29294_Cmae_Bot2011_COI	POR-A26	1	BD29294_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	ATGAACAGTCTATCCTCCTTTAG	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29294_Hsan_Wil2021_COI	POR-A26	1	BD29294_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	CCTGGGCCGGTATAGTAGGT	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.092	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29294_Mros_Gib2022_COI	POR-A26	1	BD29294_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Present	Sample	Mros_Gib2022_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29294_Msal_Bot2012_COI	POR-A26	1	BD29294_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	GYTAGTTCCRATGATGTTAGCTG	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29294_Mstr_Wil2021_COI	POR-A26	1	BD29294_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	GTAGTGGTAACTACTCATGCTTTA	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29294_Pvir_Dia2013_COI	POR-A26	1	BD29294_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	CTTAGTGGCATTAATTCGDAATCC	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29294_Rhar_Sim2018_COI	POR-A26	1	BD29294_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	CTGCTGCTATTGCTCATG	141.61454
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29296_Aamu_Bax2006_COI	POR-A24	1	BD29296_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	GCACAACCGGGATCTTTACTTC	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29296_Pcan_Bot2011_IGS	POR-A24	1	BD29296_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A24	IGS	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	CGTAATCCTCAGTACTGGCTA	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29296_Pper_Dia2013_COI	POR-A24	1	BD29296_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	CTTAGTGGCATTAATTCGDAATCC	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29296_Sspa_Oph2007_COI	POR-A24	1	BD29296_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	GAAGGCCTTCCGATGATC	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29296_Upin_Bot2011_COI	POR-A24	1	BD29296_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Upin_Bot2011_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	CTTTAATTACAGCGTTTTTATTGTTGT	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29296_Vgib_Wil2025_COI	POR-A24	1	BD29296_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	CCCCTCTTTACAAGTTCTTTAGGGT	141.6222
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29297_Asen_Wil2025_COI	POR-A23	1	BD29297_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	GTTGTTGTTACAACACATGCTCTTTTG	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29297_Cjap_Sim2018_COI	POR-A23	1	BD29297_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29297_Esin_And2018_Cyb	POR-A23	1	BD29297_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A23	Cytb	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	ACCCCTCCTCATATCCAACCA	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29297_Mgig_Bot2012_COI	POR-A23	1	BD29297_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	TCTTATTCGTTGGAGACTTTATAACCCT	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29297_Mjap_Gib2022_COI	POR-A23	1	BD29297_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	AATTTAATTGTGACTAGGCATGGTCTG	141.62089
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29298_Cmae_Bot2011_COI	POR-A22	1	BD29298_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	ATGAACAGTCTATCCTCCTTTAG	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29298_Hsan_Wil2021_COI	POR-A22	1	BD29298_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	CCTGGGCCGGTATAGTAGGT	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29298_Mros_Gib2022_COI	POR-A22	1	BD29298_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29298_Msal_Bot2012_COI	POR-A22	1	BD29298_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	GYTAGTTCCRATGATGTTAGCTG	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29298_Mstr_Wil2021_COI	POR-A22	1	BD29298_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	GTAGTGGTAACTACTCATGCTTTA	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29298_Pvir_Dia2013_COI	POR-A22	1	BD29298_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	CTTAGTGGCATTAATTCGDAATCC	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29298_Rhar_Sim2018_COI	POR-A22	1	BD29298_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	CTGCTGCTATTGCTCATG	141.61961
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29300_Aamu_Bax2006_COI	POR-A20	1	BD29300_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	GCACAACCGGGATCTTTACTTC	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29300_Pcan_Bot2011_IGS	POR-A20	1	BD29300_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A20	IGS	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	CGTAATCCTCAGTACTGGCTA	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29300_Pper_Dia2013_COI	POR-A20	1	BD29300_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	CTTAGTGGCATTAATTCGDAATCC	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.174	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29300_Sspa_Oph2007_COI	POR-A20	1	BD29300_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	GAAGGCCTTCCGATGATC	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.799	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29300_Upin_Bot2011_COI	POR-A20	1	BD29300_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Present	Sample	Upin_Bot2011_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	CTTTAATTACAGCGTTTTTATTGTTGT	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29300_Vgib_Wil2025_COI	POR-A20	1	BD29300_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	CCCCTCTTTACAAGTTCTTTAGGGT	141.61686
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29301_Asen_Wil2025_COI	POR-A19	1	BD29301_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	GTTGTTGTTACAACACATGCTCTTTTG	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29301_Cjap_Sim2018_COI	POR-A19	1	BD29301_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29301_Esin_And2018_Cyb	POR-A19	1	BD29301_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A19	Cytb	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	ACCCCTCCTCATATCCAACCA	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29301_Mgig_Bot2012_COI	POR-A19	1	BD29301_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	TCTTATTCGTTGGAGACTTTATAACCCT	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29301_Mjap_Gib2022_COI	POR-A19	1	BD29301_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	AATTTAATTGTGACTAGGCATGGTCTG	141.6153
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29302_Cmae_Bot2011_COI	POR-A18	1	BD29302_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	ATGAACAGTCTATCCTCCTTTAG	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29302_Hsan_Wil2021_COI	POR-A18	1	BD29302_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	CCTGGGCCGGTATAGTAGGT	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.397	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29302_Mros_Gib2022_COI	POR-A18	1	BD29302_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Present	Sample	Mros_Gib2022_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29302_Msal_Bot2012_COI	POR-A18	1	BD29302_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	GYTAGTTCCRATGATGTTAGCTG	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29302_Mstr_Wil2021_COI	POR-A18	1	BD29302_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	GTAGTGGTAACTACTCATGCTTTA	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29302_Pvir_Dia2013_COI	POR-A18	1	BD29302_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	CTTAGTGGCATTAATTCGDAATCC	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29302_Rhar_Sim2018_COI	POR-A18	1	BD29302_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	CTGCTGCTATTGCTCATG	141.61393
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29304_Aamu_Bax2006_COI	POR-A16	1	BD29304_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	GCACAACCGGGATCTTTACTTC	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29304_Pcan_Bot2011_IGS	POR-A16	1	BD29304_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A16	IGS	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	CGTAATCCTCAGTACTGGCTA	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29304_Pper_Dia2013_COI	POR-A16	1	BD29304_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	CTTAGTGGCATTAATTCGDAATCC	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29304_Sspa_Oph2007_COI	POR-A16	1	BD29304_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	GAAGGCCTTCCGATGATC	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29304_Upin_Bot2011_COI	POR-A16	1	BD29304_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Upin_Bot2011_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	CTTTAATTACAGCGTTTTTATTGTTGT	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29304_Vgib_Wil2025_COI	POR-A16	1	BD29304_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	CCCCTCTTTACAAGTTCTTTAGGGT	141.62101
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29305_Asen_Wil2025_COI	POR-A15	1	BD29305_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	GTTGTTGTTACAACACATGCTCTTTTG	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29305_Cjap_Sim2018_COI	POR-A15	1	BD29305_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29305_Esin_And2018_Cyb	POR-A15	1	BD29305_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A15	Cytb	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	ACCCCTCCTCATATCCAACCA	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29305_Mgig_Bot2012_COI	POR-A15	1	BD29305_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	TCTTATTCGTTGGAGACTTTATAACCCT	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29305_Mjap_Gib2022_COI	POR-A15	1	BD29305_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	AATTTAATTGTGACTAGGCATGGTCTG	141.6203
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29306_Cmae_Bot2011_COI	POR-A14	1	BD29306_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	ATGAACAGTCTATCCTCCTTTAG	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29306_Hsan_Wil2021_COI	POR-A14	1	BD29306_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	CCTGGGCCGGTATAGTAGGT	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29306_Mros_Gib2022_COI	POR-A14	1	BD29306_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29306_Msal_Bot2012_COI	POR-A14	1	BD29306_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	GYTAGTTCCRATGATGTTAGCTG	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29306_Mstr_Wil2021_COI	POR-A14	1	BD29306_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	GTAGTGGTAACTACTCATGCTTTA	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29306_Pvir_Dia2013_COI	POR-A14	1	BD29306_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	CTTAGTGGCATTAATTCGDAATCC	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29306_Rhar_Sim2018_COI	POR-A14	1	BD29306_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	CTGCTGCTATTGCTCATG	141.61892
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29308_Aamu_Bax2006_COI	POR-A12	1	BD29308_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	GCACAACCGGGATCTTTACTTC	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29308_Pcan_Bot2011_IGS	POR-A12	1	BD29308_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A12	IGS	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	CGTAATCCTCAGTACTGGCTA	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29308_Pper_Dia2013_COI	POR-A12	1	BD29308_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	CTTAGTGGCATTAATTCGDAATCC	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29308_Sspa_Oph2007_COI	POR-A12	1	BD29308_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	GAAGGCCTTCCGATGATC	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.792	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29308_Upin_Bot2011_COI	POR-A12	1	BD29308_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Present	Sample	Upin_Bot2011_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	CTTTAATTACAGCGTTTTTATTGTTGT	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29308_Vgib_Wil2025_COI	POR-A12	1	BD29308_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	CCCCTCTTTACAAGTTCTTTAGGGT	141.61615
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29309_Asen_Wil2025_COI	POR-A11	1	BD29309_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	GTTGTTGTTACAACACATGCTCTTTTG	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29309_Cjap_Sim2018_COI	POR-A11	1	BD29309_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29309_Esin_And2018_Cyb	POR-A11	1	BD29309_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A11	Cytb	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	ACCCCTCCTCATATCCAACCA	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29309_Mgig_Bot2012_COI	POR-A11	1	BD29309_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	TCTTATTCGTTGGAGACTTTATAACCCT	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29309_Mjap_Gib2022_COI	POR-A11	1	BD29309_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	AATTTAATTGTGACTAGGCATGGTCTG	141.61467
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29311_Aamu_Bax2006_COI	POR-A09	1	BD29311_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	GCACAACCGGGATCTTTACTTC	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29311_Pcan_Bot2011_IGS	POR-A09	1	BD29311_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A09	IGS	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	CGTAATCCTCAGTACTGGCTA	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29311_Pper_Dia2013_COI	POR-A09	1	BD29311_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	CTTAGTGGCATTAATTCGDAATCC	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29311_Sspa_Oph2007_COI	POR-A09	1	BD29311_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	GAAGGCCTTCCGATGATC	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.493	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29311_Upin_Bot2011_COI	POR-A09	1	BD29311_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Present	Sample	Upin_Bot2011_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	CTTTAATTACAGCGTTTTTATTGTTGT	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29311_Vgib_Wil2025_COI	POR-A09	1	BD29311_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	CCCCTCTTTACAAGTTCTTTAGGGT	141.61189
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29312_Asen_Wil2025_COI	POR-A08	1	BD29312_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	GTTGTTGTTACAACACATGCTCTTTTG	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29312_Cjap_Sim2018_COI	POR-A08	1	BD29312_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29312_Esin_And2018_Cyb	POR-A08	1	BD29312_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A08	Cytb	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	ACCCCTCCTCATATCCAACCA	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29312_Mgig_Bot2012_COI	POR-A08	1	BD29312_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	TCTTATTCGTTGGAGACTTTATAACCCT	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29312_Mjap_Gib2022_COI	POR-A08	1	BD29312_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	AATTTAATTGTGACTAGGCATGGTCTG	141.61066
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29313_Cmae_Bot2011_COI	POR-A07	1	BD29313_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	ATGAACAGTCTATCCTCCTTTAG	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29313_Hsan_Wil2021_COI	POR-A07	1	BD29313_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	CCTGGGCCGGTATAGTAGGT	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29313_Mros_Gib2022_COI	POR-A07	1	BD29313_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29313_Msal_Bot2012_COI	POR-A07	1	BD29313_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	GYTAGTTCCRATGATGTTAGCTG	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29313_Mstr_Wil2021_COI	POR-A07	1	BD29313_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	GTAGTGGTAACTACTCATGCTTTA	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29313_Pvir_Dia2013_COI	POR-A07	1	BD29313_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	CTTAGTGGCATTAATTCGDAATCC	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29313_Rhar_Sim2018_COI	POR-A07	1	BD29313_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	CTGCTGCTATTGCTCATG	141.62051
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29315_Aamu_Bax2006_COI	POR-A05	1	BD29315_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	GCACAACCGGGATCTTTACTTC	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29315_Pcan_Bot2011_IGS	POR-A05	1	BD29315_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A05	IGS	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	CGTAATCCTCAGTACTGGCTA	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29315_Pper_Dia2013_COI	POR-A05	1	BD29315_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	CTTAGTGGCATTAATTCGDAATCC	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29315_Sspa_Oph2007_COI	POR-A05	1	BD29315_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	GAAGGCCTTCCGATGATC	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.333	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29315_Upin_Bot2011_COI	POR-A05	1	BD29315_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Present	Sample	Upin_Bot2011_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	CTTTAATTACAGCGTTTTTATTGTTGT	141.61829
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29316_Cjap_Sim2018_COI	POR-A04	1	BD29316_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29316_Esin_And2018_Cyb	POR-A04	1	BD29316_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A04	Cytb	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	ACCCCTCCTCATATCCAACCA	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29316_Mgig_Bot2012_COI	POR-A04	1	BD29316_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	TCTTATTCGTTGGAGACTTTATAACCCT	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29316_Mjap_Gib2022_COI	POR-A04	1	BD29316_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	AATTTAATTGTGACTAGGCATGGTCTG	141.61684
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29317_Cmae_Bot2011_COI	POR-A03	1	BD29317_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	ATGAACAGTCTATCCTCCTTTAG	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29317_Hsan_Wil2021_COI	POR-A03	1	BD29317_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	CCTGGGCCGGTATAGTAGGT	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29317_Mros_Gib2022_COI	POR-A03	1	BD29317_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29317_Msal_Bot2012_COI	POR-A03	1	BD29317_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	GYTAGTTCCRATGATGTTAGCTG	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29317_Mstr_Wil2021_COI	POR-A03	1	BD29317_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	GTAGTGGTAACTACTCATGCTTTA	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29317_Pvir_Dia2013_COI	POR-A03	1	BD29317_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	CTTAGTGGCATTAATTCGDAATCC	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29317_Rhar_Sim2018_COI	POR-A03	1	BD29317_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	CTGCTGCTATTGCTCATG	141.61122
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29319_Aamu_Bax2006_COI	POR-A01	1	BD29319_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	GCACAACCGGGATCTTTACTTC	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29319_Pcan_Bot2011_IGS	POR-A01	1	BD29319_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A01	IGS	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	CGTAATCCTCAGTACTGGCTA	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29319_Pper_Dia2013_COI	POR-A01	1	BD29319_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	CTTAGTGGCATTAATTCGDAATCC	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29319_Sspa_Oph2007_COI	POR-A01	1	BD29319_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	GAAGGCCTTCCGATGATC	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.115	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29319_Upin_Bot2011_COI	POR-A01	1	BD29319_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Present	Sample	Upin_Bot2011_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	CTTTAATTACAGCGTTTTTATTGTTGT	141.61035
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29320_Cmae_Bot2011_COI	KPT-A35	1	BD29320_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	ATGAACAGTCTATCCTCCTTTAG	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29320_Hsan_Wil2021_COI	KPT-A35	1	BD29320_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	CCTGGGCCGGTATAGTAGGT	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29320_Mros_Gib2022_COI	KPT-A35	1	BD29320_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29320_Msal_Bot2012_COI	KPT-A35	1	BD29320_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	GYTAGTTCCRATGATGTTAGCTG	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29320_Mstr_Wil2021_COI	KPT-A35	1	BD29320_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	GTAGTGGTAACTACTCATGCTTTA	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29320_Pvir_Dia2013_COI	KPT-A35	1	BD29320_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	CTTAGTGGCATTAATTCGDAATCC	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29320_Rhar_Sim2018_COI	KPT-A35	1	BD29320_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	CTGCTGCTATTGCTCATG	137.77845
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29322_Aamu_Bax2006_COI	KPT-A33	1	BD29322_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	GCACAACCGGGATCTTTACTTC	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29322_Pcan_Bot2011_IGS	KPT-A33	1	BD29322_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A33	IGS	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	CGTAATCCTCAGTACTGGCTA	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29322_Pper_Dia2013_COI	KPT-A33	1	BD29322_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	CTTAGTGGCATTAATTCGDAATCC	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29322_Sspa_Oph2007_COI	KPT-A33	1	BD29322_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	GAAGGCCTTCCGATGATC	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29322_Upin_Bot2011_COI	KPT-A33	1	BD29322_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	CTTTAATTACAGCGTTTTTATTGTTGT	137.77745
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29323_Cjap_Sim2018_COI	KPT-A32	1	BD29323_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29323_Esin_And2018_Cyb	KPT-A32	1	BD29323_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A32	Cytb	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	ACCCCTCCTCATATCCAACCA	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29323_Mgig_Bot2012_COI	KPT-A32	1	BD29323_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	TCTTATTCGTTGGAGACTTTATAACCCT	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29323_Mjap_Gib2022_COI	KPT-A32	1	BD29323_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	AATTTAATTGTGACTAGGCATGGTCTG	137.77736
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29324_Cmae_Bot2011_COI	KPT-A31	1	BD29324_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	ATGAACAGTCTATCCTCCTTTAG	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29324_Hsan_Wil2021_COI	KPT-A31	1	BD29324_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	CCTGGGCCGGTATAGTAGGT	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29324_Mros_Gib2022_COI	KPT-A31	1	BD29324_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29324_Msal_Bot2012_COI	KPT-A31	1	BD29324_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	GYTAGTTCCRATGATGTTAGCTG	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29324_Mstr_Wil2021_COI	KPT-A31	1	BD29324_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	GTAGTGGTAACTACTCATGCTTTA	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29324_Pvir_Dia2013_COI	KPT-A31	1	BD29324_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	CTTAGTGGCATTAATTCGDAATCC	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29324_Rhar_Sim2018_COI	KPT-A31	1	BD29324_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	CTGCTGCTATTGCTCATG	137.77984
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29326_Aamu_Bax2006_COI	KPT-A29	1	BD29326_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	GCACAACCGGGATCTTTACTTC	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29326_Pcan_Bot2011_IGS	KPT-A29	1	BD29326_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A29	IGS	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	CGTAATCCTCAGTACTGGCTA	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29326_Pper_Dia2013_COI	KPT-A29	1	BD29326_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	CTTAGTGGCATTAATTCGDAATCC	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29326_Sspa_Oph2007_COI	KPT-A29	1	BD29326_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	GAAGGCCTTCCGATGATC	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29326_Upin_Bot2011_COI	KPT-A29	1	BD29326_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	CTTTAATTACAGCGTTTTTATTGTTGT	137.77712
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29327_Cjap_Sim2018_COI	KPT-A28	1	BD29327_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29327_Esin_And2018_Cyb	KPT-A28	1	BD29327_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A28	Cytb	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	ACCCCTCCTCATATCCAACCA	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29327_Mgig_Bot2012_COI	KPT-A28	1	BD29327_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	TCTTATTCGTTGGAGACTTTATAACCCT	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29327_Mjap_Gib2022_COI	KPT-A28	1	BD29327_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	AATTTAATTGTGACTAGGCATGGTCTG	137.77558
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29328_Cmae_Bot2011_COI	KPT-A27	1	BD29328_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	ATGAACAGTCTATCCTCCTTTAG	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29328_Hsan_Wil2021_COI	KPT-A27	1	BD29328_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	CCTGGGCCGGTATAGTAGGT	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29328_Mros_Gib2022_COI	KPT-A27	1	BD29328_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29328_Msal_Bot2012_COI	KPT-A27	1	BD29328_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	GYTAGTTCCRATGATGTTAGCTG	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29328_Mstr_Wil2021_COI	KPT-A27	1	BD29328_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	GTAGTGGTAACTACTCATGCTTTA	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29328_Pvir_Dia2013_COI	KPT-A27	1	BD29328_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	CTTAGTGGCATTAATTCGDAATCC	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29328_Rhar_Sim2018_COI	KPT-A27	1	BD29328_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	CTGCTGCTATTGCTCATG	137.77676
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29330_Cjap_Sim2018_COI	KPT-A25	1	BD29330_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29330_Esin_And2018_Cyb	KPT-A25	1	BD29330_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A25	Cytb	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	ACCCCTCCTCATATCCAACCA	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29330_Mgig_Bot2012_COI	KPT-A25	1	BD29330_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	TCTTATTCGTTGGAGACTTTATAACCCT	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29330_Mjap_Gib2022_COI	KPT-A25	1	BD29330_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	AATTTAATTGTGACTAGGCATGGTCTG	137.77937
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29331_Cmae_Bot2011_COI	KPT-A24	1	BD29331_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	ATGAACAGTCTATCCTCCTTTAG	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29331_Hsan_Wil2021_COI	KPT-A24	1	BD29331_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	CCTGGGCCGGTATAGTAGGT	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29331_Mros_Gib2022_COI	KPT-A24	1	BD29331_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29331_Msal_Bot2012_COI	KPT-A24	1	BD29331_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	GYTAGTTCCRATGATGTTAGCTG	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29331_Mstr_Wil2021_COI	KPT-A24	1	BD29331_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	GTAGTGGTAACTACTCATGCTTTA	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29331_Pvir_Dia2013_COI	KPT-A24	1	BD29331_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	CTTAGTGGCATTAATTCGDAATCC	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29331_Rhar_Sim2018_COI	KPT-A24	1	BD29331_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	CTGCTGCTATTGCTCATG	137.78109
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29333_Aamu_Bax2006_COI	KPT-A22	1	BD29333_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	GCACAACCGGGATCTTTACTTC	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29333_Pcan_Bot2011_IGS	KPT-A22	1	BD29333_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A22	IGS	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	CGTAATCCTCAGTACTGGCTA	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29333_Pper_Dia2013_COI	KPT-A22	1	BD29333_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	CTTAGTGGCATTAATTCGDAATCC	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29333_Sspa_Oph2007_COI	KPT-A22	1	BD29333_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	GAAGGCCTTCCGATGATC	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29333_Upin_Bot2011_COI	KPT-A22	1	BD29333_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	CTTTAATTACAGCGTTTTTATTGTTGT	137.77849
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29334_Cjap_Sim2018_COI	KPT-A21	1	BD29334_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29334_Esin_And2018_Cyb	KPT-A21	1	BD29334_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A21	Cytb	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	ACCCCTCCTCATATCCAACCA	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29334_Mgig_Bot2012_COI	KPT-A21	1	BD29334_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	TCTTATTCGTTGGAGACTTTATAACCCT	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29334_Mjap_Gib2022_COI	KPT-A21	1	BD29334_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	AATTTAATTGTGACTAGGCATGGTCTG	137.77639
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29335_Cmae_Bot2011_COI	KPT-A20	1	BD29335_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	ATGAACAGTCTATCCTCCTTTAG	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29335_Hsan_Wil2021_COI	KPT-A20	1	BD29335_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	CCTGGGCCGGTATAGTAGGT	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29335_Mros_Gib2022_COI	KPT-A20	1	BD29335_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29335_Msal_Bot2012_COI	KPT-A20	1	BD29335_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	GYTAGTTCCRATGATGTTAGCTG	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29335_Mstr_Wil2021_COI	KPT-A20	1	BD29335_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	GTAGTGGTAACTACTCATGCTTTA	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29335_Pvir_Dia2013_COI	KPT-A20	1	BD29335_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	CTTAGTGGCATTAATTCGDAATCC	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29335_Rhar_Sim2018_COI	KPT-A20	1	BD29335_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	CTGCTGCTATTGCTCATG	137.77572
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29337_Aamu_Bax2006_COI	KPT-A18	1	BD29337_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	GCACAACCGGGATCTTTACTTC	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29337_Pcan_Bot2011_IGS	KPT-A18	1	BD29337_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A18	IGS	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	CGTAATCCTCAGTACTGGCTA	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29337_Pper_Dia2013_COI	KPT-A18	1	BD29337_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	CTTAGTGGCATTAATTCGDAATCC	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29337_Sspa_Oph2007_COI	KPT-A18	1	BD29337_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	GAAGGCCTTCCGATGATC	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29337_Upin_Bot2011_COI	KPT-A18	1	BD29337_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	CTTTAATTACAGCGTTTTTATTGTTGT	137.7782
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29338_Cjap_Sim2018_COI	KPT-A17	1	BD29338_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29338_Esin_And2018_Cyb	KPT-A17	1	BD29338_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A17	Cytb	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	ACCCCTCCTCATATCCAACCA	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29338_Mgig_Bot2012_COI	KPT-A17	1	BD29338_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	TCTTATTCGTTGGAGACTTTATAACCCT	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29338_Mjap_Gib2022_COI	KPT-A17	1	BD29338_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	AATTTAATTGTGACTAGGCATGGTCTG	137.77943
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29339_Cmae_Bot2011_COI	KPT-A16	1	BD29339_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	ATGAACAGTCTATCCTCCTTTAG	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29339_Hsan_Wil2021_COI	KPT-A16	1	BD29339_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	CCTGGGCCGGTATAGTAGGT	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29339_Mros_Gib2022_COI	KPT-A16	1	BD29339_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29339_Msal_Bot2012_COI	KPT-A16	1	BD29339_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	GYTAGTTCCRATGATGTTAGCTG	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29339_Mstr_Wil2021_COI	KPT-A16	1	BD29339_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	GTAGTGGTAACTACTCATGCTTTA	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29339_Pvir_Dia2013_COI	KPT-A16	1	BD29339_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	CTTAGTGGCATTAATTCGDAATCC	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29339_Rhar_Sim2018_COI	KPT-A16	1	BD29339_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	CTGCTGCTATTGCTCATG	137.78114
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29340_Aamu_Bax2006_COI	KPT-A15	1	BD29340_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	GCACAACCGGGATCTTTACTTC	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29340_Pcan_Bot2011_IGS	KPT-A15	1	BD29340_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A15	IGS	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	CGTAATCCTCAGTACTGGCTA	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29340_Pper_Dia2013_COI	KPT-A15	1	BD29340_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	CTTAGTGGCATTAATTCGDAATCC	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29340_Sspa_Oph2007_COI	KPT-A15	1	BD29340_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	GAAGGCCTTCCGATGATC	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29340_Upin_Bot2011_COI	KPT-A15	1	BD29340_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	CTTTAATTACAGCGTTTTTATTGTTGT	137.77982
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29341_Cjap_Sim2018_COI	KPT-A14	1	BD29341_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29341_Esin_And2018_Cyb	KPT-A14	1	BD29341_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A14	Cytb	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	ACCCCTCCTCATATCCAACCA	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29341_Mgig_Bot2012_COI	KPT-A14	1	BD29341_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	TCTTATTCGTTGGAGACTTTATAACCCT	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29341_Mjap_Gib2022_COI	KPT-A14	1	BD29341_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	AATTTAATTGTGACTAGGCATGGTCTG	137.7785
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29342_Cmae_Bot2011_COI	KPT-A13	1	BD29342_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	ATGAACAGTCTATCCTCCTTTAG	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29342_Hsan_Wil2021_COI	KPT-A13	1	BD29342_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	CCTGGGCCGGTATAGTAGGT	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29342_Mros_Gib2022_COI	KPT-A13	1	BD29342_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29342_Msal_Bot2012_COI	KPT-A13	1	BD29342_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	GYTAGTTCCRATGATGTTAGCTG	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29342_Mstr_Wil2021_COI	KPT-A13	1	BD29342_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	GTAGTGGTAACTACTCATGCTTTA	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29342_Pvir_Dia2013_COI	KPT-A13	1	BD29342_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	CTTAGTGGCATTAATTCGDAATCC	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29342_Rhar_Sim2018_COI	KPT-A13	1	BD29342_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	CTGCTGCTATTGCTCATG	137.77723
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29344_Aamu_Bax2006_COI	KPT-A11	1	BD29344_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	GCACAACCGGGATCTTTACTTC	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29344_Pcan_Bot2011_IGS	KPT-A11	1	BD29344_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A11	IGS	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	CGTAATCCTCAGTACTGGCTA	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29344_Pper_Dia2013_COI	KPT-A11	1	BD29344_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	CTTAGTGGCATTAATTCGDAATCC	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29344_Sspa_Oph2007_COI	KPT-A11	1	BD29344_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	GAAGGCCTTCCGATGATC	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29344_Upin_Bot2011_COI	KPT-A11	1	BD29344_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	CTTTAATTACAGCGTTTTTATTGTTGT	137.77455
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29345_Cjap_Sim2018_COI	KPT-A10	1	BD29345_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29345_Esin_And2018_Cyb	KPT-A10	1	BD29345_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A10	Cytb	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	ACCCCTCCTCATATCCAACCA	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29345_Mgig_Bot2012_COI	KPT-A10	1	BD29345_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	TCTTATTCGTTGGAGACTTTATAACCCT	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29345_Mjap_Gib2022_COI	KPT-A10	1	BD29345_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	AATTTAATTGTGACTAGGCATGGTCTG	137.77399
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29346_Cmae_Bot2011_COI	KPT-A09	1	BD29346_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	ATGAACAGTCTATCCTCCTTTAG	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29346_Hsan_Wil2021_COI	KPT-A09	1	BD29346_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	CCTGGGCCGGTATAGTAGGT	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29346_Mros_Gib2022_COI	KPT-A09	1	BD29346_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29346_Msal_Bot2012_COI	KPT-A09	1	BD29346_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	GYTAGTTCCRATGATGTTAGCTG	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29346_Mstr_Wil2021_COI	KPT-A09	1	BD29346_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	GTAGTGGTAACTACTCATGCTTTA	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29346_Pvir_Dia2013_COI	KPT-A09	1	BD29346_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	CTTAGTGGCATTAATTCGDAATCC	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29346_Rhar_Sim2018_COI	KPT-A09	1	BD29346_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	CTGCTGCTATTGCTCATG	137.7745
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29348_Aamu_Bax2006_COI	KPT-A07	1	BD29348_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	GCACAACCGGGATCTTTACTTC	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29348_Pcan_Bot2011_IGS	KPT-A07	1	BD29348_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A07	IGS	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	CGTAATCCTCAGTACTGGCTA	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29348_Pper_Dia2013_COI	KPT-A07	1	BD29348_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	CTTAGTGGCATTAATTCGDAATCC	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29348_Sspa_Oph2007_COI	KPT-A07	1	BD29348_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	GAAGGCCTTCCGATGATC	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29348_Upin_Bot2011_COI	KPT-A07	1	BD29348_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	CTTTAATTACAGCGTTTTTATTGTTGT	137.77697
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29349_Cjap_Sim2018_COI	KPT-A06	1	BD29349_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29349_Esin_And2018_Cyb	KPT-A06	1	BD29349_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A06	Cytb	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	ACCCCTCCTCATATCCAACCA	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29349_Mgig_Bot2012_COI	KPT-A06	1	BD29349_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	TCTTATTCGTTGGAGACTTTATAACCCT	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29349_Mjap_Gib2022_COI	KPT-A06	1	BD29349_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	AATTTAATTGTGACTAGGCATGGTCTG	137.77908
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29351_Aamu_Bax2006_COI	KPT-A04	1	BD29351_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	GCACAACCGGGATCTTTACTTC	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29351_Pcan_Bot2011_IGS	KPT-A04	1	BD29351_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A04	IGS	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	CGTAATCCTCAGTACTGGCTA	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29351_Pper_Dia2013_COI	KPT-A04	1	BD29351_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	CTTAGTGGCATTAATTCGDAATCC	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29351_Sspa_Oph2007_COI	KPT-A04	1	BD29351_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	GAAGGCCTTCCGATGATC	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29351_Upin_Bot2011_COI	KPT-A04	1	BD29351_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	CTTTAATTACAGCGTTTTTATTGTTGT	137.77854
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29352_Cjap_Sim2018_COI	KPT-A03	1	BD29352_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29352_Esin_And2018_Cyb	KPT-A03	1	BD29352_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A03	Cytb	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	ACCCCTCCTCATATCCAACCA	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29352_Mgig_Bot2012_COI	KPT-A03	1	BD29352_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	TCTTATTCGTTGGAGACTTTATAACCCT	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29352_Mjap_Gib2022_COI	KPT-A03	1	BD29352_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	AATTTAATTGTGACTAGGCATGGTCTG	137.7773
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29353_Cmae_Bot2011_COI	KPT-A02	1	BD29353_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	ATGAACAGTCTATCCTCCTTTAG	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29353_Hsan_Wil2021_COI	KPT-A02	1	BD29353_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	CCTGGGCCGGTATAGTAGGT	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29353_Mros_Gib2022_COI	KPT-A02	1	BD29353_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29353_Msal_Bot2012_COI	KPT-A02	1	BD29353_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	GYTAGTTCCRATGATGTTAGCTG	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29353_Mstr_Wil2021_COI	KPT-A02	1	BD29353_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	GTAGTGGTAACTACTCATGCTTTA	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29353_Pvir_Dia2013_COI	KPT-A02	1	BD29353_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	CTTAGTGGCATTAATTCGDAATCC	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29353_Rhar_Sim2018_COI	KPT-A02	1	BD29353_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	CTGCTGCTATTGCTCATG	137.77582
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29355_Aamu_Bax2006_COI	PGI-A34	1	BD29355_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	GCACAACCGGGATCTTTACTTC	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29355_Pcan_Bot2011_IGS	PGI-A34	1	BD29355_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A34	IGS	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	CGTAATCCTCAGTACTGGCTA	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29355_Pper_Dia2013_COI	PGI-A34	1	BD29355_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	CTTAGTGGCATTAATTCGDAATCC	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29355_Sspa_Oph2007_COI	PGI-A34	1	BD29355_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	GAAGGCCTTCCGATGATC	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29355_Upin_Bot2011_COI	PGI-A34	1	BD29355_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	CTTTAATTACAGCGTTTTTATTGTTGT	137.76598
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29356_Cjap_Sim2018_COI	PGI-A33	1	BD29356_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29356_Esin_And2018_Cyb	PGI-A33	1	BD29356_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A33	Cytb	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	ACCCCTCCTCATATCCAACCA	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29356_Mgig_Bot2012_COI	PGI-A33	1	BD29356_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	TCTTATTCGTTGGAGACTTTATAACCCT	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29356_Mjap_Gib2022_COI	PGI-A33	1	BD29356_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	AATTTAATTGTGACTAGGCATGGTCTG	137.76808
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29357_Cmae_Bot2011_COI	PGI-A32	1	BD29357_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	ATGAACAGTCTATCCTCCTTTAG	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29357_Hsan_Wil2021_COI	PGI-A32	1	BD29357_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	CCTGGGCCGGTATAGTAGGT	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29357_Mros_Gib2022_COI	PGI-A32	1	BD29357_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29357_Msal_Bot2012_COI	PGI-A32	1	BD29357_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	GYTAGTTCCRATGATGTTAGCTG	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29357_Mstr_Wil2021_COI	PGI-A32	1	BD29357_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	GTAGTGGTAACTACTCATGCTTTA	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29357_Pvir_Dia2013_COI	PGI-A32	1	BD29357_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	CTTAGTGGCATTAATTCGDAATCC	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29357_Rhar_Sim2018_COI	PGI-A32	1	BD29357_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	CTGCTGCTATTGCTCATG	137.76976
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29359_Aamu_Bax2006_COI	PGI-A30	1	BD29359_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	GCACAACCGGGATCTTTACTTC	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29359_Pcan_Bot2011_IGS	PGI-A30	1	BD29359_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A30	IGS	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	CGTAATCCTCAGTACTGGCTA	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29359_Pper_Dia2013_COI	PGI-A30	1	BD29359_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	CTTAGTGGCATTAATTCGDAATCC	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29359_Sspa_Oph2007_COI	PGI-A30	1	BD29359_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	GAAGGCCTTCCGATGATC	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29359_Upin_Bot2011_COI	PGI-A30	1	BD29359_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	CTTTAATTACAGCGTTTTTATTGTTGT	137.77228
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29360_Cmae_Bot2011_COI	PGI-A29	1	BD29360_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	ATGAACAGTCTATCCTCCTTTAG	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29360_Hsan_Wil2021_COI	PGI-A29	1	BD29360_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	CCTGGGCCGGTATAGTAGGT	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29360_Mros_Gib2022_COI	PGI-A29	1	BD29360_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29360_Msal_Bot2012_COI	PGI-A29	1	BD29360_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	GYTAGTTCCRATGATGTTAGCTG	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29360_Mstr_Wil2021_COI	PGI-A29	1	BD29360_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	GTAGTGGTAACTACTCATGCTTTA	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29360_Pvir_Dia2013_COI	PGI-A29	1	BD29360_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	CTTAGTGGCATTAATTCGDAATCC	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29360_Rhar_Sim2018_COI	PGI-A29	1	BD29360_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	CTGCTGCTATTGCTCATG	137.77066
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29362_Aamu_Bax2006_COI	PGI-A27	1	BD29362_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	GCACAACCGGGATCTTTACTTC	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29362_Pcan_Bot2011_IGS	PGI-A27	1	BD29362_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A27	IGS	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	CGTAATCCTCAGTACTGGCTA	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29362_Pper_Dia2013_COI	PGI-A27	1	BD29362_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	CTTAGTGGCATTAATTCGDAATCC	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29362_Sspa_Oph2007_COI	PGI-A27	1	BD29362_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	GAAGGCCTTCCGATGATC	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29362_Upin_Bot2011_COI	PGI-A27	1	BD29362_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	CTTTAATTACAGCGTTTTTATTGTTGT	137.76747
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29363_Cjap_Sim2018_COI	PGI-A26	1	BD29363_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29363_Esin_And2018_Cyb	PGI-A26	1	BD29363_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A26	Cytb	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	ACCCCTCCTCATATCCAACCA	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29363_Mgig_Bot2012_COI	PGI-A26	1	BD29363_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	TCTTATTCGTTGGAGACTTTATAACCCT	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29363_Mjap_Gib2022_COI	PGI-A26	1	BD29363_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	AATTTAATTGTGACTAGGCATGGTCTG	137.76572
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29364_Cmae_Bot2011_COI	PGI-A25	1	BD29364_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	ATGAACAGTCTATCCTCCTTTAG	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29364_Hsan_Wil2021_COI	PGI-A25	1	BD29364_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	CCTGGGCCGGTATAGTAGGT	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29364_Mros_Gib2022_COI	PGI-A25	1	BD29364_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29364_Msal_Bot2012_COI	PGI-A25	1	BD29364_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	GYTAGTTCCRATGATGTTAGCTG	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29364_Mstr_Wil2021_COI	PGI-A25	1	BD29364_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	GTAGTGGTAACTACTCATGCTTTA	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29364_Pvir_Dia2013_COI	PGI-A25	1	BD29364_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	CTTAGTGGCATTAATTCGDAATCC	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29364_Rhar_Sim2018_COI	PGI-A25	1	BD29364_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	CTGCTGCTATTGCTCATG	137.76465
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29366_Aamu_Bax2006_COI	PGI-A23	1	BD29366_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	GCACAACCGGGATCTTTACTTC	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29366_Pcan_Bot2011_IGS	PGI-A23	1	BD29366_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A23	IGS	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	CGTAATCCTCAGTACTGGCTA	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29366_Pper_Dia2013_COI	PGI-A23	1	BD29366_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	CTTAGTGGCATTAATTCGDAATCC	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29366_Sspa_Oph2007_COI	PGI-A23	1	BD29366_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	GAAGGCCTTCCGATGATC	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29366_Upin_Bot2011_COI	PGI-A23	1	BD29366_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	CTTTAATTACAGCGTTTTTATTGTTGT	137.76796
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29367_Cjap_Sim2018_COI	PGI-A22	1	BD29367_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29367_Esin_And2018_Cyb	PGI-A22	1	BD29367_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A22	Cytb	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	ACCCCTCCTCATATCCAACCA	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29367_Mgig_Bot2012_COI	PGI-A22	1	BD29367_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	TCTTATTCGTTGGAGACTTTATAACCCT	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29367_Mjap_Gib2022_COI	PGI-A22	1	BD29367_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	AATTTAATTGTGACTAGGCATGGTCTG	137.76822
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29368_Cmae_Bot2011_COI	PGI-A21	1	BD29368_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	ATGAACAGTCTATCCTCCTTTAG	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29368_Hsan_Wil2021_COI	PGI-A21	1	BD29368_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	CCTGGGCCGGTATAGTAGGT	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29368_Mros_Gib2022_COI	PGI-A21	1	BD29368_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29368_Msal_Bot2012_COI	PGI-A21	1	BD29368_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	GYTAGTTCCRATGATGTTAGCTG	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29368_Mstr_Wil2021_COI	PGI-A21	1	BD29368_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	GTAGTGGTAACTACTCATGCTTTA	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29368_Pvir_Dia2013_COI	PGI-A21	1	BD29368_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	CTTAGTGGCATTAATTCGDAATCC	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29368_Rhar_Sim2018_COI	PGI-A21	1	BD29368_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	CTGCTGCTATTGCTCATG	137.77254
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29370_Cjap_Sim2018_COI	PGI-A19	1	BD29370_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29370_Esin_And2018_Cyb	PGI-A19	1	BD29370_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A19	Cytb	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	ACCCCTCCTCATATCCAACCA	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29370_Mgig_Bot2012_COI	PGI-A19	1	BD29370_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	TCTTATTCGTTGGAGACTTTATAACCCT	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29370_Mjap_Gib2022_COI	PGI-A19	1	BD29370_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	AATTTAATTGTGACTAGGCATGGTCTG	137.77052
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29371_Cmae_Bot2011_COI	PGI-A18	1	BD29371_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	ATGAACAGTCTATCCTCCTTTAG	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29371_Hsan_Wil2021_COI	PGI-A18	1	BD29371_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	CCTGGGCCGGTATAGTAGGT	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29371_Mros_Gib2022_COI	PGI-A18	1	BD29371_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29371_Msal_Bot2012_COI	PGI-A18	1	BD29371_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	GYTAGTTCCRATGATGTTAGCTG	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29371_Mstr_Wil2021_COI	PGI-A18	1	BD29371_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	GTAGTGGTAACTACTCATGCTTTA	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29371_Pvir_Dia2013_COI	PGI-A18	1	BD29371_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	CTTAGTGGCATTAATTCGDAATCC	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29371_Rhar_Sim2018_COI	PGI-A18	1	BD29371_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	CTGCTGCTATTGCTCATG	137.77238
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29373_Aamu_Bax2006_COI	PGI-A16	1	BD29373_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	GCACAACCGGGATCTTTACTTC	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29373_Pcan_Bot2011_IGS	PGI-A16	1	BD29373_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A16	IGS	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	CGTAATCCTCAGTACTGGCTA	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29373_Pper_Dia2013_COI	PGI-A16	1	BD29373_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	CTTAGTGGCATTAATTCGDAATCC	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29373_Sspa_Oph2007_COI	PGI-A16	1	BD29373_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	GAAGGCCTTCCGATGATC	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29373_Upin_Bot2011_COI	PGI-A16	1	BD29373_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	CTTTAATTACAGCGTTTTTATTGTTGT	137.77345
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29374_Cjap_Sim2018_COI	PGI-A15	1	BD29374_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29374_Esin_And2018_Cyb	PGI-A15	1	BD29374_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A15	Cytb	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	ACCCCTCCTCATATCCAACCA	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29374_Mgig_Bot2012_COI	PGI-A15	1	BD29374_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	TCTTATTCGTTGGAGACTTTATAACCCT	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29374_Mjap_Gib2022_COI	PGI-A15	1	BD29374_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	AATTTAATTGTGACTAGGCATGGTCTG	137.7716
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29375_Cmae_Bot2011_COI	PGI-A14	1	BD29375_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	ATGAACAGTCTATCCTCCTTTAG	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29375_Hsan_Wil2021_COI	PGI-A14	1	BD29375_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	CCTGGGCCGGTATAGTAGGT	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29375_Mros_Gib2022_COI	PGI-A14	1	BD29375_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29375_Msal_Bot2012_COI	PGI-A14	1	BD29375_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	GYTAGTTCCRATGATGTTAGCTG	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29375_Mstr_Wil2021_COI	PGI-A14	1	BD29375_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	GTAGTGGTAACTACTCATGCTTTA	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29375_Pvir_Dia2013_COI	PGI-A14	1	BD29375_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	CTTAGTGGCATTAATTCGDAATCC	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29375_Rhar_Sim2018_COI	PGI-A14	1	BD29375_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	CTGCTGCTATTGCTCATG	137.76969
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29377_Aamu_Bax2006_COI	PGI-A12	1	BD29377_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	GCACAACCGGGATCTTTACTTC	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29377_Pcan_Bot2011_IGS	PGI-A12	1	BD29377_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A12	IGS	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	CGTAATCCTCAGTACTGGCTA	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29377_Pper_Dia2013_COI	PGI-A12	1	BD29377_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	CTTAGTGGCATTAATTCGDAATCC	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29377_Sspa_Oph2007_COI	PGI-A12	1	BD29377_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	GAAGGCCTTCCGATGATC	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29377_Upin_Bot2011_COI	PGI-A12	1	BD29377_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	CTTTAATTACAGCGTTTTTATTGTTGT	137.7662
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29378_Cjap_Sim2018_COI	PGI-A11	1	BD29378_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29378_Esin_And2018_Cyb	PGI-A11	1	BD29378_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A11	Cytb	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	ACCCCTCCTCATATCCAACCA	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29378_Mgig_Bot2012_COI	PGI-A11	1	BD29378_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	TCTTATTCGTTGGAGACTTTATAACCCT	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29378_Mjap_Gib2022_COI	PGI-A11	1	BD29378_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	AATTTAATTGTGACTAGGCATGGTCTG	137.76389
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29379_Cmae_Bot2011_COI	PGI-A10	1	BD29379_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	ATGAACAGTCTATCCTCCTTTAG	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29379_Hsan_Wil2021_COI	PGI-A10	1	BD29379_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	CCTGGGCCGGTATAGTAGGT	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29379_Mros_Gib2022_COI	PGI-A10	1	BD29379_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29379_Msal_Bot2012_COI	PGI-A10	1	BD29379_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	GYTAGTTCCRATGATGTTAGCTG	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29379_Mstr_Wil2021_COI	PGI-A10	1	BD29379_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	GTAGTGGTAACTACTCATGCTTTA	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29379_Pvir_Dia2013_COI	PGI-A10	1	BD29379_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	CTTAGTGGCATTAATTCGDAATCC	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29379_Rhar_Sim2018_COI	PGI-A10	1	BD29379_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	CTGCTGCTATTGCTCATG	137.76517
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29380_Aamu_Bax2006_COI	PGI-A09	1	BD29380_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	GCACAACCGGGATCTTTACTTC	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29380_Pcan_Bot2011_IGS	PGI-A09	1	BD29380_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A09	IGS	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	CGTAATCCTCAGTACTGGCTA	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29380_Pper_Dia2013_COI	PGI-A09	1	BD29380_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	CTTAGTGGCATTAATTCGDAATCC	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29380_Sspa_Oph2007_COI	PGI-A09	1	BD29380_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	GAAGGCCTTCCGATGATC	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29380_Upin_Bot2011_COI	PGI-A09	1	BD29380_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	CTTTAATTACAGCGTTTTTATTGTTGT	137.76659
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29381_Cjap_Sim2018_COI	PGI-A08	1	BD29381_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29381_Esin_And2018_Cyb	PGI-A08	1	BD29381_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A08	Cytb	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	ACCCCTCCTCATATCCAACCA	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29381_Mgig_Bot2012_COI	PGI-A08	1	BD29381_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	TCTTATTCGTTGGAGACTTTATAACCCT	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29381_Mjap_Gib2022_COI	PGI-A08	1	BD29381_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	AATTTAATTGTGACTAGGCATGGTCTG	137.76859
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29382_Cmae_Bot2011_COI	PGI-A07	1	BD29382_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	ATGAACAGTCTATCCTCCTTTAG	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29382_Hsan_Wil2021_COI	PGI-A07	1	BD29382_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	CCTGGGCCGGTATAGTAGGT	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29382_Mros_Gib2022_COI	PGI-A07	1	BD29382_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29382_Msal_Bot2012_COI	PGI-A07	1	BD29382_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	GYTAGTTCCRATGATGTTAGCTG	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29382_Mstr_Wil2021_COI	PGI-A07	1	BD29382_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	GTAGTGGTAACTACTCATGCTTTA	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29382_Pvir_Dia2013_COI	PGI-A07	1	BD29382_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	CTTAGTGGCATTAATTCGDAATCC	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29382_Rhar_Sim2018_COI	PGI-A07	1	BD29382_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	CTGCTGCTATTGCTCATG	137.77035
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29384_Aamu_Bax2006_COI	PGI-A02	1	BD29384_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	GCACAACCGGGATCTTTACTTC	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29384_Pcan_Bot2011_IGS	PGI-A02	1	BD29384_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A02	IGS	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	CGTAATCCTCAGTACTGGCTA	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29384_Pper_Dia2013_COI	PGI-A02	1	BD29384_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	CTTAGTGGCATTAATTCGDAATCC	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29384_Sspa_Oph2007_COI	PGI-A02	1	BD29384_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	GAAGGCCTTCCGATGATC	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29384_Upin_Bot2011_COI	PGI-A02	1	BD29384_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	CTTTAATTACAGCGTTTTTATTGTTGT	137.76551
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29385_Cjap_Sim2018_COI	PGI-A03	1	BD29385_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29385_Esin_And2018_Cyb	PGI-A03	1	BD29385_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A03	Cytb	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	ACCCCTCCTCATATCCAACCA	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29385_Mgig_Bot2012_COI	PGI-A03	1	BD29385_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	TCTTATTCGTTGGAGACTTTATAACCCT	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29385_Mjap_Gib2022_COI	PGI-A03	1	BD29385_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	AATTTAATTGTGACTAGGCATGGTCTG	137.76775
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29386_Cmae_Bot2011_COI	PGI-A05	1	BD29386_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	ATGAACAGTCTATCCTCCTTTAG	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29386_Hsan_Wil2021_COI	PGI-A05	1	BD29386_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	CCTGGGCCGGTATAGTAGGT	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29386_Mros_Gib2022_COI	PGI-A05	1	BD29386_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29386_Msal_Bot2012_COI	PGI-A05	1	BD29386_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	GYTAGTTCCRATGATGTTAGCTG	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29386_Mstr_Wil2021_COI	PGI-A05	1	BD29386_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	GTAGTGGTAACTACTCATGCTTTA	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29386_Pvir_Dia2013_COI	PGI-A05	1	BD29386_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	CTTAGTGGCATTAATTCGDAATCC	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29386_Rhar_Sim2018_COI	PGI-A05	1	BD29386_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	CTGCTGCTATTGCTCATG	137.77132
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29388_Aamu_Bax2006_COI	PGI-A01	1	BD29388_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	GCACAACCGGGATCTTTACTTC	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29388_Pcan_Bot2011_IGS	PGI-A01	1	BD29388_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A01	IGS	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	CGTAATCCTCAGTACTGGCTA	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29388_Pper_Dia2013_COI	PGI-A01	1	BD29388_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	CTTAGTGGCATTAATTCGDAATCC	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29388_Sspa_Oph2007_COI	PGI-A01	1	BD29388_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	GAAGGCCTTCCGATGATC	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29388_Upin_Bot2011_COI	PGI-A01	1	BD29388_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	CTTTAATTACAGCGTTTTTATTGTTGT	137.76432
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29389_Cjap_Sim2018_COI	KPT-A01	1	BD29389_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29389_Esin_And2018_Cyb	KPT-A01	1	BD29389_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A01	Cytb	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	ACCCCTCCTCATATCCAACCA	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29389_Mgig_Bot2012_COI	KPT-A01	1	BD29389_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	TCTTATTCGTTGGAGACTTTATAACCCT	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29389_Mjap_Gib2022_COI	KPT-A01	1	BD29389_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	AATTTAATTGTGACTAGGCATGGTCTG	137.7746
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82108727	WGS84	BD42627	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	37.547	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42627_Asen_Wil2025_COI	ADV-01	0	BD42627_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Present	Sample	Asen_Wil2025_COI	ADV-01	COI	45	BD42627	1.00E-05	Nil	Australia	ADV-01	GTTGTTGTTACAACACATGCTCTTTTG	144.9092464
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42627_Cjap_Sim2018_COI	ADV-01	1	BD42627_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-01	COI	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9092464
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42627_Esin_And2018_Cyb	ADV-01	1	BD42627_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-01	Cytb	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	ACCCCTCCTCATATCCAACCA	144.9092464
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42627_Mjap_Gib2022_COI	ADV-01	1	BD42627_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-01	COI	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	AATTTAATTGTGACTAGGCATGGTCTG	144.9092464
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42628_Hsan_Wil2021_COI	ADV-02	1	BD42628_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-02	COI	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	CCTGGGCCGGTATAGTAGGT	144.9096108
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42628_Mros_Gib2022_COI	ADV-02	1	BD42628_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-02	COI	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9096108
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42628_Pvir_Dia2013_COI	ADV-02	1	BD42628_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-02	COI	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	CTTAGTGGCATTAATTCGDAATCC	144.9096108
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42628_Rhar_Sim2018_COI	ADV-02	1	BD42628_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-02	COI	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	CTGCTGCTATTGCTCATG	144.9096108
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82080701	WGS84	BD42630	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.117	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42630_Asen_Wil2025_COI	ADV-04	0	BD42630_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Present	Sample	Asen_Wil2025_COI	ADV-04	COI	45	BD42630	1.00E-05	Nil	Australia	ADV-04	GTTGTTGTTACAACACATGCTCTTTTG	144.9090642
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42630_Cjap_Sim2018_COI	ADV-04	1	BD42630_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-04	COI	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9090642
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42630_Esin_And2018_Cyb	ADV-04	1	BD42630_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-04	Cytb	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	ACCCCTCCTCATATCCAACCA	144.9090642
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42630_Mjap_Gib2022_COI	ADV-04	1	BD42630_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-04	COI	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	AATTTAATTGTGACTAGGCATGGTCTG	144.9090642
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	32.297	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42631_Hsan_Wil2021_COI	ADV-05	1	BD42631_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-05	COI	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	CCTGGGCCGGTATAGTAGGT	144.9088819
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42631_Mros_Gib2022_COI	ADV-05	1	BD42631_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-05	COI	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9088819
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42631_Pvir_Dia2013_COI	ADV-05	1	BD42631_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-05	COI	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	CTTAGTGGCATTAATTCGDAATCC	144.9088819
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42631_Rhar_Sim2018_COI	ADV-05	1	BD42631_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-05	COI	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	CTGCTGCTATTGCTCATG	144.9088819
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42633_Pper_Dia2013_COI	ADV-07	1	BD42633_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-07	COI	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	CTTAGTGGCATTAATTCGDAATCC	144.8992714
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82764214	WGS84	BD42633	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42633_Vgib_Wil2025_COI	ADV-07	0	BD42633_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-07	COI	45	BD42633	1.00E-05	Nil	Australia	ADV-07	CCCCTCTTTACAAGTTCTTTAGGGT	144.8992714
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82777317	WGS84	BD42634	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.968	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42634_Asen_Wil2025_COI	ADV-08	0	BD42634_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Present	Sample	Asen_Wil2025_COI	ADV-08	COI	45	BD42634	1.00E-05	Nil	Australia	ADV-08	GTTGTTGTTACAACACATGCTCTTTTG	144.8995694
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42634_Cjap_Sim2018_COI	ADV-08	1	BD42634_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-08	COI	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.8995694
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42634_Esin_And2018_Cyb	ADV-08	1	BD42634_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-08	Cytb	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	ACCCCTCCTCATATCCAACCA	144.8995694
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42634_Mjap_Gib2022_COI	ADV-08	1	BD42634_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-08	COI	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	AATTTAATTGTGACTAGGCATGGTCTG	144.8995694
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.482	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42635_Hsan_Wil2021_COI	ADV-09	1	BD42635_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-09	COI	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	CCTGGGCCGGTATAGTAGGT	144.9001655
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42635_Mros_Gib2022_COI	ADV-09	1	BD42635_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-09	COI	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9001655
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42635_Pvir_Dia2013_COI	ADV-09	1	BD42635_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-09	COI	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	CTTAGTGGCATTAATTCGDAATCC	144.9001655
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42635_Rhar_Sim2018_COI	ADV-09	1	BD42635_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-09	COI	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	CTGCTGCTATTGCTCATG	144.9001655
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42637_Pper_Dia2013_COI	ADV-11	1	BD42637_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-11	COI	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	CTTAGTGGCATTAATTCGDAATCC	144.9004635
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82816625	WGS84	BD42637	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42637_Vgib_Wil2025_COI	ADV-11	0	BD42637_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-11	COI	45	BD42637	1.00E-05	Nil	Australia	ADV-11	CCCCTCTTTACAAGTTCTTTAGGGT	144.9004635
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84243228	WGS84	BD42638	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	32.729	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42638_Asen_Wil2025_COI	ADV-12	0	BD42638_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Present	Sample	Asen_Wil2025_COI	ADV-12	COI	45	BD42638	1.00E-05	Nil	Australia	ADV-12	GTTGTTGTTACAACACATGCTCTTTTG	144.9287502
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42638_Cjap_Sim2018_COI	ADV-12	1	BD42638_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-12	COI	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9287502
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42638_Esin_And2018_Cyb	ADV-12	1	BD42638_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-12	Cytb	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	ACCCCTCCTCATATCCAACCA	144.9287502
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42638_Mjap_Gib2022_COI	ADV-12	1	BD42638_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-12	COI	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	AATTTAATTGTGACTAGGCATGGTCTG	144.9287502
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	30.27	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42639_Hsan_Wil2021_COI	ADV-13	1	BD42639_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-13	COI	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	CCTGGGCCGGTATAGTAGGT	144.9298511
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42639_Mros_Gib2022_COI	ADV-13	1	BD42639_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-13	COI	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9298511
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42639_Pvir_Dia2013_COI	ADV-13	1	BD42639_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-13	COI	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	CTTAGTGGCATTAATTCGDAATCC	144.9298511
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42639_Rhar_Sim2018_COI	ADV-13	1	BD42639_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-13	COI	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	CTGCTGCTATTGCTCATG	144.9298511
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42640_Pper_Dia2013_COI	ADV-14	1	BD42640_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-14	COI	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	CTTAGTGGCATTAATTCGDAATCC	144.9293007
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84254362	WGS84	BD42640	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42640_Vgib_Wil2025_COI	ADV-14	0	BD42640_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-14	COI	45	BD42640	1.00E-05	Nil	Australia	ADV-14	CCCCTCTTTACAAGTTCTTTAGGGT	144.9293007
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84232086	WGS84	BD42641	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	35.035	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42641_Asen_Wil2025_COI	ADV-15	0	BD42641_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Present	Sample	Asen_Wil2025_COI	ADV-15	COI	45	BD42641	1.00E-05	Nil	Australia	ADV-15	GTTGTTGTTACAACACATGCTCTTTTG	144.9281998
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42641_Cjap_Sim2018_COI	ADV-15	1	BD42641_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-15	COI	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9281998
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42641_Esin_And2018_Cyb	ADV-15	1	BD42641_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-15	Cytb	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	ACCCCTCCTCATATCCAACCA	144.9281998
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42641_Mjap_Gib2022_COI	ADV-15	1	BD42641_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-15	COI	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	AATTTAATTGTGACTAGGCATGGTCTG	144.9281998
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	32.896	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42642_Hsan_Wil2021_COI	ADV-16	1	BD42642_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-16	COI	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	CCTGGGCCGGTATAGTAGGT	144.9276494
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42642_Mros_Gib2022_COI	ADV-16	1	BD42642_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-16	COI	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9276494
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42642_Pvir_Dia2013_COI	ADV-16	1	BD42642_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-16	COI	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	CTTAGTGGCATTAATTCGDAATCC	144.9276494
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42642_Rhar_Sim2018_COI	ADV-16	1	BD42642_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-16	COI	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	CTGCTGCTATTGCTCATG	144.9276494
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42644_Pper_Dia2013_COI	ADV-18	1	BD42644_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-18	COI	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	CTTAGTGGCATTAATTCGDAATCC	144.9216141
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81843466	WGS84	BD42644	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42644_Vgib_Wil2025_COI	ADV-18	0	BD42644_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-18	COI	45	BD42644	1.00E-05	Nil	Australia	ADV-18	CCCCTCTTTACAAGTTCTTTAGGGT	144.9216141
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81895614	WGS84	BD42645	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42645_Asen_Wil2025_COI	ADV-19	0	BD42645_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-19	COI	45	BD42645	1.00E-05	Nil	Australia	ADV-19	GTTGTTGTTACAACACATGCTCTTTTG	144.9217938
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42645_Cjap_Sim2018_COI	ADV-19	1	BD42645_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-19	COI	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9217938
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42645_Esin_And2018_Cyb	ADV-19	1	BD42645_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-19	Cytb	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	ACCCCTCCTCATATCCAACCA	144.9217938
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42645_Mjap_Gib2022_COI	ADV-19	1	BD42645_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-19	COI	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	AATTTAATTGTGACTAGGCATGGTCTG	144.9217938
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42646_Hsan_Wil2021_COI	ADV-20	1	BD42646_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-20	COI	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	CCTGGGCCGGTATAGTAGGT	144.9218836
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42646_Mros_Gib2022_COI	ADV-20	1	BD42646_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-20	COI	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9218836
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42646_Pvir_Dia2013_COI	ADV-20	1	BD42646_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-20	COI	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	CTTAGTGGCATTAATTCGDAATCC	144.9218836
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42646_Rhar_Sim2018_COI	ADV-20	1	BD42646_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-20	COI	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	CTGCTGCTATTGCTCATG	144.9218836
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42648_Pper_Dia2013_COI	ADV-22	1	BD42648_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-22	COI	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	CTTAGTGGCATTAATTCGDAATCC	144.9153674
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81919597	WGS84	BD42648	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42648_Vgib_Wil2025_COI	ADV-22	0	BD42648_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-22	COI	45	BD42648	1.00E-05	Nil	Australia	ADV-22	CCCCTCTTTACAAGTTCTTTAGGGT	144.9153674
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81944634	WGS84	BD42649	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.814	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42649_Asen_Wil2025_COI	ADV-23	0	BD42649_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Present	Sample	Asen_Wil2025_COI	ADV-23	COI	45	BD42649	1.00E-05	Nil	Australia	ADV-23	GTTGTTGTTACAACACATGCTCTTTTG	144.9154956
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42649_Cjap_Sim2018_COI	ADV-23	1	BD42649_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-23	COI	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9154956
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42649_Esin_And2018_Cyb	ADV-23	1	BD42649_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-23	Cytb	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	ACCCCTCCTCATATCCAACCA	144.9154956
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42649_Mjap_Gib2022_COI	ADV-23	1	BD42649_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-23	COI	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	AATTTAATTGTGACTAGGCATGGTCTG	144.9154956
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42651_Pper_Dia2013_COI	ADV-25	1	BD42651_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-25	COI	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	CTTAGTGGCATTAATTCGDAATCC	144.9158804
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8201976	WGS84	BD42651	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42651_Vgib_Wil2025_COI	ADV-25	0	BD42651_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-25	COI	45	BD42651	1.00E-05	Nil	Australia	ADV-25	CCCCTCTTTACAAGTTCTTTAGGGT	144.9158804
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84537488	WGS84	BD42652	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.122	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42652_Asen_Wil2025_COI	ADV-30	0	BD42652_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Present	Sample	Asen_Wil2025_COI	ADV-30	COI	45	BD42652	1.00E-05	Nil	Australia	ADV-30	GTTGTTGTTACAACACATGCTCTTTTG	144.906424
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42652_Cjap_Sim2018_COI	ADV-30	1	BD42652_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-30	COI	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.906424
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42652_Esin_And2018_Cyb	ADV-30	1	BD42652_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-30	Cytb	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	ACCCCTCCTCATATCCAACCA	144.906424
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42652_Mjap_Gib2022_COI	ADV-30	1	BD42652_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-30	COI	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	AATTTAATTGTGACTAGGCATGGTCTG	144.906424
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42653_Hsan_Wil2021_COI	ADV-29	1	BD42653_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-29	COI	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	CCTGGGCCGGTATAGTAGGT	144.9067328
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42653_Mros_Gib2022_COI	ADV-29	1	BD42653_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-29	COI	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9067328
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42653_Pvir_Dia2013_COI	ADV-29	1	BD42653_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-29	COI	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	CTTAGTGGCATTAATTCGDAATCC	144.9067328
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42653_Rhar_Sim2018_COI	ADV-29	1	BD42653_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-29	COI	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	CTGCTGCTATTGCTCATG	144.9067328
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42655_Pper_Dia2013_COI	ADV-27	1	BD42655_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-27	COI	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	CTTAGTGGCATTAATTCGDAATCC	144.9076592
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8449172	WGS84	BD42655	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42655_Vgib_Wil2025_COI	ADV-27	0	BD42655_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-27	COI	45	BD42655	1.00E-05	Nil	Australia	ADV-27	CCCCTCTTTACAAGTTCTTTAGGGT	144.9076592
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84514602	WGS84	BD42656	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42656_Asen_Wil2025_COI	ADV-26	0	BD42656_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-26	COI	45	BD42656	1.00E-05	Nil	Australia	ADV-26	GTTGTTGTTACAACACATGCTCTTTTG	144.9070416
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42656_Cjap_Sim2018_COI	ADV-26	1	BD42656_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-26	COI	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9070416
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42656_Esin_And2018_Cyb	ADV-26	1	BD42656_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-26	Cytb	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	ACCCCTCCTCATATCCAACCA	144.9070416
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42656_Mjap_Gib2022_COI	ADV-26	1	BD42656_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-26	COI	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	AATTTAATTGTGACTAGGCATGGTCTG	144.9070416
BD44107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55408	WGS84	BD44107	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44107_Vgib_Wil2025_COI	ESAV-211	0	BD44107_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44107	Sample	BD44107	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-211	COI	45	BD44107	1.00E-05	Nil	Australia	ESAV-211	CCCCTCTTTACAAGTTCTTTAGGGT	147.8808
BD44108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55406	WGS84	BD44108	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	32.933	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44108_Mjap_Gib2022_COI	ESAV-212	1	BD44108_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44108	Sample	BD44108	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-212	COI	45	BD44108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-212	AATTTAATTGTGACTAGGCATGGTCTG	147.87962
BD44109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55448	WGS84	BD44109	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44109_Mros_Gib2022_COI	ESAV-213	1	BD44109_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44109	Sample	BD44109	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-213	COI	45	BD44109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-213	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87745
BD44111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55539	WGS84	BD44111	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44111_Asen_Wil2025_COI	ESAV-215	0	BD44111_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44111	Sample	BD44111	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-215	COI	45	BD44111	1.00E-05	Nil	Australia	ESAV-215	GTTGTTGTTACAACACATGCTCTTTTG	147.87551
BD44111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55539	WGS84	BD44111	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	38.484	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44111_Mjap_Gib2022_COI	ESAV-215	1	BD44111_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44111	Sample	BD44111	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-215	COI	45	BD44111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-215	AATTTAATTGTGACTAGGCATGGTCTG	147.87551
BD44112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55723	WGS84	BD44112	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44112_Mros_Gib2022_COI	ESAV-216	1	BD44112_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44112	Sample	BD44112	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-216	COI	45	BD44112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-216	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87394
BD44115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54758	WGS84	BD44115	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	32.242	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44115_Mjap_Gib2022_COI	ESAV-219	1	BD44115_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44115	Sample	BD44115	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-219	COI	45	BD44115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-219	AATTTAATTGTGACTAGGCATGGTCTG	147.92946
BD44116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54629	WGS84	BD44116	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	33.331	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44116_Mros_Gib2022_COI	ESAV-220	1	BD44116_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44116	Sample	BD44116	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-220	COI	45	BD44116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-220	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.92917
BD44119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15041	WGS84	BD44119	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44119_Asen_Wil2025_COI	ESAV-204	0	BD44119_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44119	Sample	BD44119	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-204	COI	45	BD44119	1.00E-05	Nil	Australia	ESAV-204	GTTGTTGTTACAACACATGCTCTTTTG	147.28248
BD44119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15041	WGS84	BD44119	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44119_Mjap_Gib2022_COI	ESAV-204	1	BD44119_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44119	Sample	BD44119	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-204	COI	45	BD44119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-204	AATTTAATTGTGACTAGGCATGGTCTG	147.28248
BD44122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55356	WGS84	BD44122	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44122_Mjap_Gib2022_COI	ESAV-226	1	BD44122_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44122	Sample	BD44122	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-226	COI	45	BD44122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-226	AATTTAATTGTGACTAGGCATGGTCTG	147.89482
BD44123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55084	WGS84	BD44123	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	36.109	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44123_Mros_Gib2022_COI	ESAV-225	1	BD44123_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44123	Sample	BD44123	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-225	COI	45	BD44123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-225	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.90735
BD44125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15187	WGS84	BD44125	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	34.402	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44125_Vgib_Wil2025_COI	ESAV-205	0	BD44125_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44125	Sample	BD44125	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-205	COI	45	BD44125	1.00E-05	Nil	Australia	ESAV-205	CCCCTCTTTACAAGTTCTTTAGGGT	147.28325
BD44126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15334	WGS84	BD44126	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.424	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44126_Asen_Wil2025_COI	ESAV-206	0	BD44126_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44126	Sample	BD44126	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-206	COI	45	BD44126	1.00E-05	Nil	Australia	ESAV-206	GTTGTTGTTACAACACATGCTCTTTTG	147.28351
BD44126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15334	WGS84	BD44126	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44126_Mjap_Gib2022_COI	ESAV-206	1	BD44126_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44126	Sample	BD44126	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-206	COI	45	BD44126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-206	AATTTAATTGTGACTAGGCATGGTCTG	147.28351
BD44127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15435	WGS84	BD44127	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.468	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44127_Mros_Gib2022_COI	ESAV-207	1	BD44127_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44127	Sample	BD44127	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-207	COI	45	BD44127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-207	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.2836
BD44129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15662	WGS84	BD44129	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.663	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44129_Vgib_Wil2025_COI	ESAV-209	0	BD44129_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44129	Sample	BD44129	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-209	COI	45	BD44129	1.00E-05	Nil	Australia	ESAV-209	CCCCTCTTTACAAGTTCTTTAGGGT	147.28031
BD44130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15936	WGS84	BD44130	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	36.641	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44130_Mros_Gib2022_COI	ESAV-210	1	BD44130_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44130	Sample	BD44130	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-210	COI	45	BD44130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-210	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.27943
BD44132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1546	WGS84	BD44132	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.293	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44132_Vgib_Wil2025_COI	ESAV-193	0	BD44132_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44132	Sample	BD44132	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-193	COI	45	BD44132	1.00E-05	Nil	Australia	ESAV-193	CCCCTCTTTACAAGTTCTTTAGGGT	147.28539
BD44133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15661	WGS84	BD44133	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	37.344	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44133_Asen_Wil2025_COI	ESAV-194	0	BD44133_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44133	Sample	BD44133	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-194	COI	45	BD44133	1.00E-05	Nil	Australia	ESAV-194	GTTGTTGTTACAACACATGCTCTTTTG	147.28693
BD44133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15661	WGS84	BD44133	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44133_Mjap_Gib2022_COI	ESAV-194	1	BD44133_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44133	Sample	BD44133	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-194	COI	45	BD44133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-194	AATTTAATTGTGACTAGGCATGGTCTG	147.28693
BD44134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15551	WGS84	BD44134	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.599	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44134_Mros_Gib2022_COI	ESAV-196	1	BD44134_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44134	Sample	BD44134	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-196	COI	45	BD44134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-196	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.289
BD44136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15645	WGS84	BD44136	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.838	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44136_Vgib_Wil2025_COI	ESAV-199	0	BD44136_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44136	Sample	BD44136	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-199	COI	45	BD44136	1.00E-05	Nil	Australia	ESAV-199	CCCCTCTTTACAAGTTCTTTAGGGT	147.29038
BD44137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15657	WGS84	BD44137	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44137_Asen_Wil2025_COI	ESAV-201	0	BD44137_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44137	Sample	BD44137	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-201	COI	45	BD44137	1.00E-05	Nil	Australia	ESAV-201	GTTGTTGTTACAACACATGCTCTTTTG	147.28881
BD44137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15657	WGS84	BD44137	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44137_Mjap_Gib2022_COI	ESAV-201	1	BD44137_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44137	Sample	BD44137	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-201	COI	45	BD44137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-201	AATTTAATTGTGACTAGGCATGGTCTG	147.28881
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44138_Hsan_Wil2021_COI	ESAV-001	1	BD44138_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-001	COI	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	CCTGGGCCGGTATAGTAGGT	147.24663
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.317	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44138_Mros_Gib2022_COI	ESAV-001	1	BD44138_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-001	COI	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.24663
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44138_Pvir_Dia2013_COI	ESAV-001	1	BD44138_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-001	COI	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	CTTAGTGGCATTAATTCGDAATCC	147.24663
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44138_Rhar_Sim2018_COI	ESAV-001	1	BD44138_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-001	COI	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	CTGCTGCTATTGCTCATG	147.24663
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15882	WGS84	BD44140	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.418	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44140_Asen_Wil2025_COI	ESAV-003	0	BD44140_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-003	COI	45	BD44140	1.00E-05	Nil	Australia	ESAV-003	GTTGTTGTTACAACACATGCTCTTTTG	147.25183
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44140_Cjap_Sim2018_COI	ESAV-003	1	BD44140_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-003	COI	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.25183
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44140_Esin_And2018_Cyb	ESAV-003	1	BD44140_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-003	Cytb	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	ACCCCTCCTCATATCCAACCA	147.25183
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44140_Mjap_Gib2022_COI	ESAV-003	1	BD44140_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-003	COI	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	AATTTAATTGTGACTAGGCATGGTCTG	147.25183
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44141_Hsan_Wil2021_COI	ESAV-004	1	BD44141_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-004	COI	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	CCTGGGCCGGTATAGTAGGT	147.25625
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.428	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44141_Mros_Gib2022_COI	ESAV-004	1	BD44141_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-004	COI	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.25625
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44141_Pvir_Dia2013_COI	ESAV-004	1	BD44141_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-004	COI	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	CTTAGTGGCATTAATTCGDAATCC	147.25625
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44141_Rhar_Sim2018_COI	ESAV-004	1	BD44141_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-004	COI	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	CTGCTGCTATTGCTCATG	147.25625
BD44143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1618	WGS84	BD44143	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.962	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44143_Vgib_Wil2025_COI	ESAV-006	0	BD44143_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44143	Sample	BD44143	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-006	COI	45	BD44143	1.00E-05	Nil	Australia	ESAV-006	CCCCTCTTTACAAGTTCTTTAGGGT	147.26127
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.16088	WGS84	BD44144	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44144_Asen_Wil2025_COI	ESAV-007	0	BD44144_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-007	COI	45	BD44144	1.00E-05	Nil	Australia	ESAV-007	GTTGTTGTTACAACACATGCTCTTTTG	147.25872
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44144_Cjap_Sim2018_COI	ESAV-007	1	BD44144_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-007	COI	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.25872
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44144_Esin_And2018_Cyb	ESAV-007	1	BD44144_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-007	Cytb	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	ACCCCTCCTCATATCCAACCA	147.25872
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44144_Mjap_Gib2022_COI	ESAV-007	1	BD44144_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-007	COI	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	AATTTAATTGTGACTAGGCATGGTCTG	147.25872
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44145_Hsan_Wil2021_COI	ESAV-008	1	BD44145_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-008	COI	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	CCTGGGCCGGTATAGTAGGT	147.28853
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.416	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44145_Mros_Gib2022_COI	ESAV-008	1	BD44145_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-008	COI	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28853
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44145_Pvir_Dia2013_COI	ESAV-008	1	BD44145_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-008	COI	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	CTTAGTGGCATTAATTCGDAATCC	147.28853
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44145_Rhar_Sim2018_COI	ESAV-008	1	BD44145_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-008	COI	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	CTGCTGCTATTGCTCATG	147.28853
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44147_Pper_Dia2013_COI	ESAV-010	1	BD44147_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-010	COI	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	CTTAGTGGCATTAATTCGDAATCC	147.28753
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15468	WGS84	BD44147	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.153	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44147_Vgib_Wil2025_COI	ESAV-010	0	BD44147_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-010	COI	45	BD44147	1.00E-05	Nil	Australia	ESAV-010	CCCCTCTTTACAAGTTCTTTAGGGT	147.28753
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15335	WGS84	BD44148	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.155	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44148_Asen_Wil2025_COI	ESAV-011	0	BD44148_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-011	COI	45	BD44148	1.00E-05	Nil	Australia	ESAV-011	GTTGTTGTTACAACACATGCTCTTTTG	147.28832
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44148_Cjap_Sim2018_COI	ESAV-011	1	BD44148_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-011	COI	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.28832
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44148_Esin_And2018_Cyb	ESAV-011	1	BD44148_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-011	Cytb	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	ACCCCTCCTCATATCCAACCA	147.28832
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44148_Mjap_Gib2022_COI	ESAV-011	1	BD44148_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-011	COI	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	AATTTAATTGTGACTAGGCATGGTCTG	147.28832
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44149_Hsan_Wil2021_COI	ESAV-012	1	BD44149_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-012	COI	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	CCTGGGCCGGTATAGTAGGT	147.28903
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	26.693	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44149_Mros_Gib2022_COI	ESAV-012	1	BD44149_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-012	COI	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28903
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44149_Pvir_Dia2013_COI	ESAV-012	1	BD44149_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-012	COI	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	CTTAGTGGCATTAATTCGDAATCC	147.28903
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44149_Rhar_Sim2018_COI	ESAV-012	1	BD44149_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-012	COI	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	CTGCTGCTATTGCTCATG	147.28903
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44150_Pper_Dia2013_COI	ESAV-013	1	BD44150_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-013	COI	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	CTTAGTGGCATTAATTCGDAATCC	147.28998
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15132	WGS84	BD44150	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.246	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44150_Vgib_Wil2025_COI	ESAV-013	0	BD44150_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-013	COI	45	BD44150	1.00E-05	Nil	Australia	ESAV-013	CCCCTCTTTACAAGTTCTTTAGGGT	147.28998
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15225	WGS84	BD44151	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44151_Asen_Wil2025_COI	ESAV-014	0	BD44151_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-014	COI	45	BD44151	1.00E-05	Nil	Australia	ESAV-014	GTTGTTGTTACAACACATGCTCTTTTG	147.2887
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44151_Cjap_Sim2018_COI	ESAV-014	1	BD44151_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-014	COI	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.2887
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44151_Esin_And2018_Cyb	ESAV-014	1	BD44151_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-014	Cytb	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	ACCCCTCCTCATATCCAACCA	147.2887
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44151_Mjap_Gib2022_COI	ESAV-014	1	BD44151_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-014	COI	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	AATTTAATTGTGACTAGGCATGGTCTG	147.2887
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44152_Hsan_Wil2021_COI	ESAV-015	1	BD44152_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-015	COI	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	CCTGGGCCGGTATAGTAGGT	147.28717
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.665	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44152_Mros_Gib2022_COI	ESAV-015	1	BD44152_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-015	COI	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28717
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44152_Pvir_Dia2013_COI	ESAV-015	1	BD44152_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-015	COI	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	CTTAGTGGCATTAATTCGDAATCC	147.28717
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44152_Rhar_Sim2018_COI	ESAV-015	1	BD44152_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-015	COI	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	CTGCTGCTATTGCTCATG	147.28717
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44154_Pper_Dia2013_COI	ESAV-018	1	BD44154_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-018	COI	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	CTTAGTGGCATTAATTCGDAATCC	147.8818
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44155_Cjap_Sim2018_COI	ESAV-019	1	BD44155_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-019	COI	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.88082
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44155_Esin_And2018_Cyb	ESAV-019	1	BD44155_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-019	Cytb	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	ACCCCTCCTCATATCCAACCA	147.88082
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	35.237	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44155_Mjap_Gib2022_COI	ESAV-019	1	BD44155_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-019	COI	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	AATTTAATTGTGACTAGGCATGGTCTG	147.88082
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44156_Hsan_Wil2021_COI	ESAV-020	1	BD44156_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-020	COI	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	CCTGGGCCGGTATAGTAGGT	147.87966
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.044	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44156_Mros_Gib2022_COI	ESAV-020	1	BD44156_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-020	COI	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87966
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44156_Pvir_Dia2013_COI	ESAV-020	1	BD44156_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-020	COI	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	CTTAGTGGCATTAATTCGDAATCC	147.87966
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44156_Rhar_Sim2018_COI	ESAV-020	1	BD44156_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-020	COI	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	CTGCTGCTATTGCTCATG	147.87966
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44158_Pper_Dia2013_COI	ESAV-025	1	BD44158_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-025	COI	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	CTTAGTGGCATTAATTCGDAATCC	147.87623
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44159_Cjap_Sim2018_COI	ESAV-028	1	BD44159_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-028	COI	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87426
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44159_Esin_And2018_Cyb	ESAV-028	1	BD44159_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-028	Cytb	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	ACCCCTCCTCATATCCAACCA	147.87426
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44159_Mjap_Gib2022_COI	ESAV-028	1	BD44159_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-028	COI	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	AATTTAATTGTGACTAGGCATGGTCTG	147.87426
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44161_Pper_Dia2013_COI	ESAV-032	1	BD44161_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-032	COI	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	CTTAGTGGCATTAATTCGDAATCC	147.92961
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44162_Cjap_Sim2018_COI	ESAV-033	1	BD44162_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-033	COI	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.92926
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44162_Esin_And2018_Cyb	ESAV-033	1	BD44162_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-033	Cytb	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	ACCCCTCCTCATATCCAACCA	147.92926
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44162_Mjap_Gib2022_COI	ESAV-033	1	BD44162_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-033	COI	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	AATTTAATTGTGACTAGGCATGGTCTG	147.92926
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44163_Hsan_Wil2021_COI	ESAV-034	1	BD44163_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-034	COI	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	CCTGGGCCGGTATAGTAGGT	147.91372
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	26.124	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44163_Mros_Gib2022_COI	ESAV-034	1	BD44163_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-034	COI	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.91372
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44163_Pvir_Dia2013_COI	ESAV-034	1	BD44163_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-034	COI	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	CTTAGTGGCATTAATTCGDAATCC	147.91372
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44163_Rhar_Sim2018_COI	ESAV-034	1	BD44163_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-034	COI	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	CTGCTGCTATTGCTCATG	147.91372
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44165_Pper_Dia2013_COI	ESAV-036	1	BD44165_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-036	COI	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	CTTAGTGGCATTAATTCGDAATCC	147.91501
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44166_Cjap_Sim2018_COI	ESAV-037	1	BD44166_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-037	COI	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.9156
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44166_Esin_And2018_Cyb	ESAV-037	1	BD44166_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-037	Cytb	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	ACCCCTCCTCATATCCAACCA	147.9156
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	36.1	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44166_Mjap_Gib2022_COI	ESAV-037	1	BD44166_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-037	COI	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	AATTTAATTGTGACTAGGCATGGTCTG	147.9156
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44167_Hsan_Wil2021_COI	ESAV-038	1	BD44167_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-038	COI	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	CCTGGGCCGGTATAGTAGGT	147.91067
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.764	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44167_Mros_Gib2022_COI	ESAV-038	1	BD44167_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-038	COI	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.91067
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44167_Pvir_Dia2013_COI	ESAV-038	1	BD44167_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-038	COI	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	CTTAGTGGCATTAATTCGDAATCC	147.91067
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44167_Rhar_Sim2018_COI	ESAV-038	1	BD44167_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-038	COI	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	CTGCTGCTATTGCTCATG	147.91067
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD44709_Aamu_Bax2006_COI	POR-B24	1	BD44709_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	GCACAACCGGGATCTTTACTTC	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD44709_Pcan_Bot2011_IGS	POR-B24	1	BD44709_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B24	IGS	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	CGTAATCCTCAGTACTGGCTA	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44709_Pper_Dia2013_COI	POR-B24	1	BD44709_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	CTTAGTGGCATTAATTCGDAATCC	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD44709_Sspa_Oph2007_COI	POR-B24	1	BD44709_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	GAAGGCCTTCCGATGATC	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.683	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD44709_Upin_Bot2011_COI	POR-B24	1	BD44709_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Present	Sample	Upin_Bot2011_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	CTTTAATTACAGCGTTTTTATTGTTGT	141.61416
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD44710_Cmae_Bot2011_COI	POR-B25	1	BD44710_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	ATGAACAGTCTATCCTCCTTTAG	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44710_Hsan_Wil2021_COI	POR-B25	1	BD44710_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	CCTGGGCCGGTATAGTAGGT	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44710_Mros_Gib2022_COI	POR-B25	1	BD44710_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD44710_Msal_Bot2012_COI	POR-B25	1	BD44710_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	GYTAGTTCCRATGATGTTAGCTG	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD44710_Mstr_Wil2021_COI	POR-B25	1	BD44710_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	GTAGTGGTAACTACTCATGCTTTA	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44710_Pvir_Dia2013_COI	POR-B25	1	BD44710_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	CTTAGTGGCATTAATTCGDAATCC	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44710_Rhar_Sim2018_COI	POR-B25	1	BD44710_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	CTGCTGCTATTGCTCATG	141.61558
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD44712_Aamu_Bax2006_COI	POR-B27	1	BD44712_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	GCACAACCGGGATCTTTACTTC	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD44712_Pcan_Bot2011_IGS	POR-B27	1	BD44712_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B27	IGS	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	CGTAATCCTCAGTACTGGCTA	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44712_Pper_Dia2013_COI	POR-B27	1	BD44712_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	CTTAGTGGCATTAATTCGDAATCC	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD44712_Sspa_Oph2007_COI	POR-B27	1	BD44712_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	GAAGGCCTTCCGATGATC	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.529	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD44712_Upin_Bot2011_COI	POR-B27	1	BD44712_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Present	Sample	Upin_Bot2011_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	CTTTAATTACAGCGTTTTTATTGTTGT	141.61831
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44713_Cjap_Sim2018_COI	POR-B28	1	BD44713_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44713_Esin_And2018_Cyb	POR-B28	1	BD44713_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B28	Cytb	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	ACCCCTCCTCATATCCAACCA	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD44713_Mgig_Bot2012_COI	POR-B28	1	BD44713_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	TCTTATTCGTTGGAGACTTTATAACCCT	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44713_Mjap_Gib2022_COI	POR-B28	1	BD44713_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	AATTTAATTGTGACTAGGCATGGTCTG	141.61972
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD44714_Cmae_Bot2011_COI	POR-B35	1	BD44714_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	ATGAACAGTCTATCCTCCTTTAG	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44714_Hsan_Wil2021_COI	POR-B35	1	BD44714_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	CCTGGGCCGGTATAGTAGGT	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	30.653	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44714_Mros_Gib2022_COI	POR-B35	1	BD44714_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Present	Sample	Mros_Gib2022_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD44714_Msal_Bot2012_COI	POR-B35	1	BD44714_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	GYTAGTTCCRATGATGTTAGCTG	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD44714_Mstr_Wil2021_COI	POR-B35	1	BD44714_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	GTAGTGGTAACTACTCATGCTTTA	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44714_Pvir_Dia2013_COI	POR-B35	1	BD44714_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	CTTAGTGGCATTAATTCGDAATCC	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44714_Rhar_Sim2018_COI	POR-B35	1	BD44714_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	CTGCTGCTATTGCTCATG	141.61984
BD47587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81045	WGS84	BD47587	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47587_Esin_And2018_Cyb	ESAV-170	1	BD47587_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47587	Sample	BD47587	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-170	Cytb	45	BD47587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-170	ACCCCTCCTCATATCCAACCA	138.48379
BD47587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81045	WGS84	BD47587	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47587_Mjap_Gib2022_COI	ESAV-170	1	BD47587_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47587	Sample	BD47587	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-170	COI	45	BD47587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-170	AATTTAATTGTGACTAGGCATGGTCTG	138.48379
BD47590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83042	WGS84	BD47590	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47590_Esin_And2018_Cyb	ESAV-062	1	BD47590_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47590	Sample	BD47590	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-062	Cytb	45	BD47590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-062	ACCCCTCCTCATATCCAACCA	138.46219
BD47590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83042	WGS84	BD47590	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47590_Mjap_Gib2022_COI	ESAV-062	1	BD47590_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47590	Sample	BD47590	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-062	COI	45	BD47590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-062	AATTTAATTGTGACTAGGCATGGTCTG	138.46219
BD47593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82665	WGS84	BD47593	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47593_Vgib_Wil2025_COI	GC39	1	BD47593_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47593	Sample	BD47593	targeted	Absent	Sample	Vgib_Wil2025_COI	GC39	COI	45	BD47593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC39	CCCCTCTTTACAAGTTCTTTAGGGT	151.24658
BD47594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.0209	WGS84	BD47594	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47594_Esin_And2018_Cyb	ESAV-233	1	BD47594_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47594	Sample	BD47594	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-233	Cytb	45	BD47594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-233	ACCCCTCCTCATATCCAACCA	137.76756
BD47594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.0209	WGS84	BD47594	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47594_Mjap_Gib2022_COI	ESAV-233	1	BD47594_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47594	Sample	BD47594	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-233	COI	45	BD47594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-233	AATTTAATTGTGACTAGGCATGGTCTG	137.76756
BD47597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81301	WGS84	BD47597	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47597_Vgib_Wil2025_COI	ESAV-083	1	BD47597_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47597	Sample	BD47597	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-083	COI	45	BD47597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-083	CCCCTCTTTACAAGTTCTTTAGGGT	138.46399
BD47598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82129	WGS84	BD47598	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47598_Esin_And2018_Cyb	ESAV-072	1	BD47598_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47598	Sample	BD47598	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-072	Cytb	45	BD47598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-072	ACCCCTCCTCATATCCAACCA	138.46305
BD47598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82129	WGS84	BD47598	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47598_Mjap_Gib2022_COI	ESAV-072	1	BD47598_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47598	Sample	BD47598	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-072	COI	45	BD47598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-072	AATTTAATTGTGACTAGGCATGGTCTG	138.46305
BD47602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81023	WGS84	BD47602	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47602_Esin_And2018_Cyb	ESAV-084	1	BD47602_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47602	Sample	BD47602	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-084	Cytb	45	BD47602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-084	ACCCCTCCTCATATCCAACCA	138.46412
BD47602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81023	WGS84	BD47602	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47602_Mjap_Gib2022_COI	ESAV-084	1	BD47602_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47602	Sample	BD47602	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-084	COI	45	BD47602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-084	AATTTAATTGTGACTAGGCATGGTCTG	138.46412
BD47605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86448	WGS84	BD47605	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47605_Pcan_Bot2011_IGS	ESAV-122	1	BD47605_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47605	Sample	BD47605	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-122	IGS	45	BD47605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-122	CGTAATCCTCAGTACTGGCTA	138.46828
BD47606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86602	WGS84	BD47606	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47606_Esin_And2018_Cyb	ESAV-111	1	BD47606_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47606	Sample	BD47606	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-111	Cytb	45	BD47606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-111	ACCCCTCCTCATATCCAACCA	138.46543
BD47606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86602	WGS84	BD47606	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47606_Mjap_Gib2022_COI	ESAV-111	1	BD47606_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47606	Sample	BD47606	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-111	COI	45	BD47606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-111	AATTTAATTGTGACTAGGCATGGTCTG	138.46543
BD47612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.85544	WGS84	BD47612	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47612_Vgib_Wil2025_COI	GC13	1	BD47612_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47612	Sample	BD47612	targeted	Absent	Sample	Vgib_Wil2025_COI	GC13	COI	45	BD47612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC13	CCCCTCTTTACAAGTTCTTTAGGGT	151.32222
BD47613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80642	WGS84	BD47613	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47613_Esin_And2018_Cyb	GD44	1	BD47613_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47613	Sample	BD47613	targeted	Absent	Sample	Esin_And2018_Cyb	GD44	Cytb	45	BD47613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD44	ACCCCTCCTCATATCCAACCA	151.21991
BD47613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80642	WGS84	BD47613	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47613_Mjap_Gib2022_COI	GD44	1	BD47613_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47613	Sample	BD47613	targeted	Absent	Sample	Mjap_Gib2022_COI	GD44	COI	45	BD47613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD44	AATTTAATTGTGACTAGGCATGGTCTG	151.21991
BD47616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.71821	WGS84	BD47616	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47616_Vgib_Wil2025_COI	ESAV-189	1	BD47616_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47616	Sample	BD47616	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-189	COI	45	BD47616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-189	CCCCTCTTTACAAGTTCTTTAGGGT	135.88066
BD47617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80134	WGS84	BD47617	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47617_Esin_And2018_Cyb	ESAV-177	1	BD47617_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47617	Sample	BD47617	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-177	Cytb	45	BD47617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-177	ACCCCTCCTCATATCCAACCA	138.48248
BD47617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80134	WGS84	BD47617	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47617_Mjap_Gib2022_COI	ESAV-177	1	BD47617_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47617	Sample	BD47617	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-177	COI	45	BD47617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-177	AATTTAATTGTGACTAGGCATGGTCTG	138.48248
BD47620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84862	WGS84	BD47620	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47620_Esin_And2018_Cyb	ESAV-094	1	BD47620_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47620	Sample	BD47620	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-094	Cytb	45	BD47620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-094	ACCCCTCCTCATATCCAACCA	138.46662
BD47620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84862	WGS84	BD47620	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47620_Mjap_Gib2022_COI	ESAV-094	1	BD47620_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47620	Sample	BD47620	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-094	COI	45	BD47620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-094	AATTTAATTGTGACTAGGCATGGTCTG	138.46662
BD47623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82193	WGS84	BD47623	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47623_Vgib_Wil2025_COI	GA21	1	BD47623_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47623	Sample	BD47623	targeted	Absent	Sample	Vgib_Wil2025_COI	GA21	COI	45	BD47623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA21	CCCCTCTTTACAAGTTCTTTAGGGT	151.24014
BD47627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81919	WGS84	BD47627	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47627_Pper_Dia2013_COI	GC68	1	BD47627_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47627	Sample	BD47627	targeted	Absent	Sample	Pper_Dia2013_COI	GC68	COI	45	BD47627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC68	CTTAGTGGCATTAATTCGDAATCC	151.2429
BD47627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81919	WGS84	BD47627	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47627_Vgib_Wil2025_COI	GC68	1	BD47627_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47627	Sample	BD47627	targeted	Absent	Sample	Vgib_Wil2025_COI	GC68	COI	45	BD47627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC68	CCCCTCTTTACAAGTTCTTTAGGGT	151.2429
BD47628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81925	WGS84	BD47628	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47628_Esin_And2018_Cyb	GC52	1	BD47628_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47628	Sample	BD47628	targeted	Absent	Sample	Esin_And2018_Cyb	GC52	Cytb	45	BD47628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC52	ACCCCTCCTCATATCCAACCA	151.23972
BD47630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80554	WGS84	BD47630	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47630_Pcan_Bot2011_IGS	GC102	1	BD47630_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47630	Sample	BD47630	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC102	IGS	45	BD47630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC102	CGTAATCCTCAGTACTGGCTA	151.2154
BD47630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80554	WGS84	BD47630	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47630_Pper_Dia2013_COI	GC102	1	BD47630_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47630	Sample	BD47630	targeted	Absent	Sample	Pper_Dia2013_COI	GC102	COI	45	BD47630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC102	CTTAGTGGCATTAATTCGDAATCC	151.2154
BD47630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80554	WGS84	BD47630	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47630_Vgib_Wil2025_COI	GC102	1	BD47630_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47630	Sample	BD47630	targeted	Absent	Sample	Vgib_Wil2025_COI	GC102	COI	45	BD47630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC102	CCCCTCTTTACAAGTTCTTTAGGGT	151.2154
BD47634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.95629	WGS84	BD47634	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47634_Pcan_Bot2011_IGS	KPT-A40	1	BD47634_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47634	Sample	BD47634	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A40	IGS	45	BD47634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A40	CGTAATCCTCAGTACTGGCTA	137.77862
BD47634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.95629	WGS84	BD47634	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47634_Pper_Dia2013_COI	KPT-A40	1	BD47634_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47634	Sample	BD47634	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A40	COI	45	BD47634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A40	CTTAGTGGCATTAATTCGDAATCC	137.77862
BD47634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.95629	WGS84	BD47634	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47634_Vgib_Wil2025_COI	KPT-A40	1	BD47634_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47634	Sample	BD47634	targeted	Absent	Sample	Vgib_Wil2025_COI	KPT-A40	COI	45	BD47634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A40	CCCCTCTTTACAAGTTCTTTAGGGT	137.77862
BD47638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.8499	WGS84	BD47638	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47638_Pcan_Bot2011_IGS	ESAV-095	1	BD47638_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47638	Sample	BD47638	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-095	IGS	45	BD47638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-095	CGTAATCCTCAGTACTGGCTA	138.4664
BD47638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.8499	WGS84	BD47638	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47638_Pper_Dia2013_COI	ESAV-095	1	BD47638_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47638	Sample	BD47638	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-095	COI	45	BD47638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-095	CTTAGTGGCATTAATTCGDAATCC	138.4664
BD47638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.8499	WGS84	BD47638	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47638_Vgib_Wil2025_COI	ESAV-095	1	BD47638_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47638	Sample	BD47638	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-095	COI	45	BD47638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-095	CCCCTCTTTACAAGTTCTTTAGGGT	138.4664
BD47641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78406	WGS84	BD47641	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47641_Pcan_Bot2011_IGS	GC79	1	BD47641_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47641	Sample	BD47641	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC79	IGS	45	BD47641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC79	CGTAATCCTCAGTACTGGCTA	151.20079
BD47641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78406	WGS84	BD47641	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47641_Pper_Dia2013_COI	GC79	1	BD47641_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47641	Sample	BD47641	targeted	Absent	Sample	Pper_Dia2013_COI	GC79	COI	45	BD47641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC79	CTTAGTGGCATTAATTCGDAATCC	151.20079
BD47641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78406	WGS84	BD47641	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47641_Vgib_Wil2025_COI	GC79	1	BD47641_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47641	Sample	BD47641	targeted	Absent	Sample	Vgib_Wil2025_COI	GC79	COI	45	BD47641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC79	CCCCTCTTTACAAGTTCTTTAGGGT	151.20079
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47642_Asen_Wil2025_COI	GC28	1	BD47642_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Asen_Wil2025_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	GTTGTTGTTACAACACATGCTCTTTTG	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47642_Cjap_Sim2018_COI	GC28	1	BD47642_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Cjap_Sim2018_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47642_Esin_And2018_Cyb	GC28	1	BD47642_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Esin_And2018_Cyb	GC28	Cytb	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	ACCCCTCCTCATATCCAACCA	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47642_Mjap_Gib2022_COI	GC28	1	BD47642_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Mjap_Gib2022_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	AATTTAATTGTGACTAGGCATGGTCTG	151.23943
BD47645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82913	WGS84	BD47645	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47645_Pcan_Bot2011_IGS	ESAV-149	1	BD47645_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47645	Sample	BD47645	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-149	IGS	45	BD47645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-149	CGTAATCCTCAGTACTGGCTA	138.4748
BD47645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82913	WGS84	BD47645	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47645_Pper_Dia2013_COI	ESAV-149	1	BD47645_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47645	Sample	BD47645	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-149	COI	45	BD47645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-149	CTTAGTGGCATTAATTCGDAATCC	138.4748
BD47645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82913	WGS84	BD47645	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47645_Vgib_Wil2025_COI	ESAV-149	1	BD47645_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47645	Sample	BD47645	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-149	COI	45	BD47645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-149	CCCCTCTTTACAAGTTCTTTAGGGT	138.4748
BD47646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.8368	WGS84	BD47646	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47646_Esin_And2018_Cyb	ESAV-055	1	BD47646_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47646	Sample	BD47646	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-055	Cytb	45	BD47646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-055	ACCCCTCCTCATATCCAACCA	138.46281
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47649_Pcan_Bot2011_IGS	GC22	1	BD47649_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC22	IGS	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	CGTAATCCTCAGTACTGGCTA	151.25658
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47649_Pper_Dia2013_COI	GC22	1	BD47649_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Pper_Dia2013_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	CTTAGTGGCATTAATTCGDAATCC	151.25658
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47649_Vgib_Wil2025_COI	GC22	1	BD47649_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Vgib_Wil2025_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	CCCCTCTTTACAAGTTCTTTAGGGT	151.25658
BD47652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86236	WGS84	BD47652	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47652_Pcan_Bot2011_IGS	ESAV-124	1	BD47652_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47652	Sample	BD47652	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-124	IGS	45	BD47652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-124	CGTAATCCTCAGTACTGGCTA	138.46894
BD47652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86236	WGS84	BD47652	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47652_Pper_Dia2013_COI	ESAV-124	1	BD47652_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47652	Sample	BD47652	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-124	COI	45	BD47652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-124	CTTAGTGGCATTAATTCGDAATCC	138.46894
BD47652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86236	WGS84	BD47652	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47652_Vgib_Wil2025_COI	ESAV-124	1	BD47652_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47652	Sample	BD47652	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-124	COI	45	BD47652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-124	CCCCTCTTTACAAGTTCTTTAGGGT	138.46894
BD47653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83202	WGS84	BD47653	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47653_Esin_And2018_Cyb	GC38	1	BD47653_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47653	Sample	BD47653	targeted	Absent	Sample	Esin_And2018_Cyb	GC38	Cytb	45	BD47653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC38	ACCCCTCCTCATATCCAACCA	151.26034
BD47656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.79769	WGS84	BD47656	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47656_Pcan_Bot2011_IGS	ESAV-181	1	BD47656_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47656	Sample	BD47656	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-181	IGS	45	BD47656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-181	CGTAATCCTCAGTACTGGCTA	138.48051
BD47656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.79769	WGS84	BD47656	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47656_Pper_Dia2013_COI	ESAV-181	1	BD47656_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47656	Sample	BD47656	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-181	COI	45	BD47656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-181	CTTAGTGGCATTAATTCGDAATCC	138.48051
BD47656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.79769	WGS84	BD47656	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47656_Vgib_Wil2025_COI	ESAV-181	1	BD47656_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47656	Sample	BD47656	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-181	COI	45	BD47656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-181	CCCCTCTTTACAAGTTCTTTAGGGT	138.48051
BD47657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80774	WGS84	BD47657	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47657_Esin_And2018_Cyb	ESAV-171	1	BD47657_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47657	Sample	BD47657	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-171	Cytb	45	BD47657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-171	ACCCCTCCTCATATCCAACCA	138.48428
BD47660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83589	WGS84	BD47660	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47660_Esin_And2018_Cyb	ESAV-056	1	BD47660_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47660	Sample	BD47660	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-056	Cytb	45	BD47660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-056	ACCCCTCCTCATATCCAACCA	138.46256
BD47660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83589	WGS84	BD47660	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47660_Mjap_Gib2022_COI	ESAV-056	1	BD47660_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47660	Sample	BD47660	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-056	COI	45	BD47660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-056	AATTTAATTGTGACTAGGCATGGTCTG	138.46256
BD47661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78689	WGS84	BD47661	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47661_Msal_Bot2012_COI	GB49	1	BD47661_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47661	Sample	BD47661	targeted	Absent	Sample	Msal_Bot2012_COI	GB49	COI	45	BD47661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB49	GYTAGTTCCRATGATGTTAGCTG	151.17419
BD47663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83458	WGS84	BD47663	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47663_Pcan_Bot2011_IGS	GA42	1	BD47663_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47663	Sample	BD47663	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA42	IGS	45	BD47663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA42	CGTAATCCTCAGTACTGGCTA	151.26904
BD47663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83458	WGS84	BD47663	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47663_Pper_Dia2013_COI	GA42	1	BD47663_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47663	Sample	BD47663	targeted	Absent	Sample	Pper_Dia2013_COI	GA42	COI	45	BD47663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA42	CTTAGTGGCATTAATTCGDAATCC	151.26904
BD47663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83458	WGS84	BD47663	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47663_Vgib_Wil2025_COI	GA42	1	BD47663_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47663	Sample	BD47663	targeted	Absent	Sample	Vgib_Wil2025_COI	GA42	COI	45	BD47663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA42	CCCCTCTTTACAAGTTCTTTAGGGT	151.26904
BD47665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81464	WGS84	BD47665	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47665_Hsan_Wil2021_COI	ESAV-165	1	BD47665_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47665	Sample	BD47665	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-165	COI	45	BD47665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-165	CCTGGGCCGGTATAGTAGGT	138.48375
BD47665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81464	WGS84	BD47665	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47665_Msal_Bot2012_COI	ESAV-165	1	BD47665_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47665	Sample	BD47665	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-165	COI	45	BD47665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-165	GYTAGTTCCRATGATGTTAGCTG	138.48375
BD47665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81464	WGS84	BD47665	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47665_Pvir_Dia2013_COI	ESAV-165	1	BD47665_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47665	Sample	BD47665	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-165	COI	45	BD47665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-165	CTTAGTGGCATTAATTCGDAATCC	138.48375
BD47669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83318	WGS84	BD47669	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47669_Hsan_Wil2021_COI	ESAV-059	1	BD47669_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47669	Sample	BD47669	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-059	COI	45	BD47669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-059	CCTGGGCCGGTATAGTAGGT	138.46252
BD47669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83318	WGS84	BD47669	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47669_Msal_Bot2012_COI	ESAV-059	1	BD47669_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47669	Sample	BD47669	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-059	COI	45	BD47669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-059	GYTAGTTCCRATGATGTTAGCTG	138.46252
BD47669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83318	WGS84	BD47669	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47669_Pvir_Dia2013_COI	ESAV-059	1	BD47669_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47669	Sample	BD47669	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-059	COI	45	BD47669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-059	CTTAGTGGCATTAATTCGDAATCC	138.46252
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47671_Asen_Wil2025_COI	GB67	1	BD47671_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Asen_Wil2025_COI	GB67	COI	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	GTTGTTGTTACAACACATGCTCTTTTG	151.24434
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47671_Mjap_Gib2022_COI	GB67	1	BD47671_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Mjap_Gib2022_COI	GB67	COI	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	AATTTAATTGTGACTAGGCATGGTCTG	151.24434
BD47672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83651	WGS84	BD47672	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47672_Hsan_Wil2021_COI	GB16	1	BD47672_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47672	Sample	BD47672	targeted	Absent	Sample	Hsan_Wil2021_COI	GB16	COI	45	BD47672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB16	CCTGGGCCGGTATAGTAGGT	151.27227
BD47672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83651	WGS84	BD47672	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47672_Msal_Bot2012_COI	GB16	1	BD47672_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47672	Sample	BD47672	targeted	Absent	Sample	Msal_Bot2012_COI	GB16	COI	45	BD47672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB16	GYTAGTTCCRATGATGTTAGCTG	151.27227
BD47672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83651	WGS84	BD47672	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47672_Pvir_Dia2013_COI	GB16	1	BD47672_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47672	Sample	BD47672	targeted	Absent	Sample	Pvir_Dia2013_COI	GB16	COI	45	BD47672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB16	CTTAGTGGCATTAATTCGDAATCC	151.27227
BD47676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86697	WGS84	BD47676	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47676_Hsan_Wil2021_COI	ESAV-112	1	BD47676_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47676	Sample	BD47676	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-112	COI	45	BD47676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-112	CCTGGGCCGGTATAGTAGGT	138.46522
BD47676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86697	WGS84	BD47676	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47676_Msal_Bot2012_COI	ESAV-112	1	BD47676_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47676	Sample	BD47676	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-112	COI	45	BD47676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-112	GYTAGTTCCRATGATGTTAGCTG	138.46522
BD47676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86697	WGS84	BD47676	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47676_Pvir_Dia2013_COI	ESAV-112	1	BD47676_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47676	Sample	BD47676	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-112	COI	45	BD47676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-112	CTTAGTGGCATTAATTCGDAATCC	138.46522
BD47681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82023	WGS84	BD47681	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47681_Pcan_Bot2011_IGS	MA80	1	BD47681_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47681	Sample	BD47681	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA80	IGS	45	BD47681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA80	CGTAATCCTCAGTACTGGCTA	151.2409
BD47681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82023	WGS84	BD47681	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47681_Vgib_Wil2025_COI	MA80	1	BD47681_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47681	Sample	BD47681	targeted	Absent	Sample	Vgib_Wil2025_COI	MA80	COI	45	BD47681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA80	CCCCTCTTTACAAGTTCTTTAGGGT	151.2409
BD47683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82469	WGS84	BD47683	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47683_Hsan_Wil2021_COI	GC33	1	BD47683_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47683	Sample	BD47683	targeted	Absent	Sample	Hsan_Wil2021_COI	GC33	COI	45	BD47683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC33	CCTGGGCCGGTATAGTAGGT	151.24528
BD47683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82469	WGS84	BD47683	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47683_Msal_Bot2012_COI	GC33	1	BD47683_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47683	Sample	BD47683	targeted	Absent	Sample	Msal_Bot2012_COI	GC33	COI	45	BD47683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC33	GYTAGTTCCRATGATGTTAGCTG	151.24528
BD47683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82469	WGS84	BD47683	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47683_Pvir_Dia2013_COI	GC33	1	BD47683_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47683	Sample	BD47683	targeted	Absent	Sample	Pvir_Dia2013_COI	GC33	COI	45	BD47683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC33	CTTAGTGGCATTAATTCGDAATCC	151.24528
BD47687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80433	WGS84	BD47687	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47687_Hsan_Wil2021_COI	ESAV-174	1	BD47687_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47687	Sample	BD47687	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-174	COI	45	BD47687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-174	CCTGGGCCGGTATAGTAGGT	138.48364
BD47687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80433	WGS84	BD47687	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47687_Msal_Bot2012_COI	ESAV-174	1	BD47687_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47687	Sample	BD47687	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-174	COI	45	BD47687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-174	GYTAGTTCCRATGATGTTAGCTG	138.48364
BD47687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80433	WGS84	BD47687	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47687_Pvir_Dia2013_COI	ESAV-174	1	BD47687_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47687	Sample	BD47687	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-174	COI	45	BD47687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-174	CTTAGTGGCATTAATTCGDAATCC	138.48364
BD47690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82582	WGS84	BD47690	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47690_Hsan_Wil2021_COI	ESAV-067	1	BD47690_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47690	Sample	BD47690	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-067	COI	45	BD47690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-067	CCTGGGCCGGTATAGTAGGT	138.46249
BD47690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82582	WGS84	BD47690	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47690_Msal_Bot2012_COI	ESAV-067	1	BD47690_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47690	Sample	BD47690	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-067	COI	45	BD47690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-067	GYTAGTTCCRATGATGTTAGCTG	138.46249
BD47690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82582	WGS84	BD47690	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47690_Pvir_Dia2013_COI	ESAV-067	1	BD47690_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47690	Sample	BD47690	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-067	COI	45	BD47690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-067	CTTAGTGGCATTAATTCGDAATCC	138.46249
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47693_Asen_Wil2025_COI	GB78	1	BD47693_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Asen_Wil2025_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	GTTGTTGTTACAACACATGCTCTTTTG	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47693_Cjap_Sim2018_COI	GB78	1	BD47693_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Cjap_Sim2018_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47693_Esin_And2018_Cyb	GB78	1	BD47693_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Esin_And2018_Cyb	GB78	Cytb	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	ACCCCTCCTCATATCCAACCA	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47693_Mjap_Gib2022_COI	GB78	1	BD47693_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Mjap_Gib2022_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	AATTTAATTGTGACTAGGCATGGTCTG	151.23999
BD47694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14767	WGS84	BD47694	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47694_Hsan_Wil2021_COI	THE-A38	1	BD47694_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47694	Sample	BD47694	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A38	COI	45	BD47694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A38	CCTGGGCCGGTATAGTAGGT	133.64068
BD47694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14767	WGS84	BD47694	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47694_Msal_Bot2012_COI	THE-A38	1	BD47694_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47694	Sample	BD47694	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A38	COI	45	BD47694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A38	GYTAGTTCCRATGATGTTAGCTG	133.64068
BD47694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14767	WGS84	BD47694	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47694_Pvir_Dia2013_COI	THE-A38	1	BD47694_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47694	Sample	BD47694	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A38	COI	45	BD47694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A38	CTTAGTGGCATTAATTCGDAATCC	133.64068
BD47696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82028	WGS84	BD47696	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47696_Vgib_Wil2025_COI	ESAV-159	1	BD47696_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47696	Sample	BD47696	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-159	COI	45	BD47696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-159	CCCCTCTTTACAAGTTCTTTAGGGT	138.48196
BD47698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86024	WGS84	BD47698	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47698_Hsan_Wil2021_COI	ESAV-104	1	BD47698_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47698	Sample	BD47698	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-104	COI	45	BD47698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-104	CCTGGGCCGGTATAGTAGGT	138.46511
BD47698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86024	WGS84	BD47698	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47698_Msal_Bot2012_COI	ESAV-104	1	BD47698_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47698	Sample	BD47698	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-104	COI	45	BD47698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-104	GYTAGTTCCRATGATGTTAGCTG	138.46511
BD47700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83225	WGS84	BD47700	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47700_Vgib_Wil2025_COI	ESAV-060	1	BD47700_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47700	Sample	BD47700	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-060	COI	45	BD47700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-060	CCCCTCTTTACAAGTTCTTTAGGGT	138.46248
BD47701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84218	WGS84	BD47701	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47701_Asen_Wil2025_COI	ESAV-049	1	BD47701_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47701	Sample	BD47701	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-049	COI	45	BD47701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-049	GTTGTTGTTACAACACATGCTCTTTTG	138.4638
BD47702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79201	WGS84	BD47702	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47702_Hsan_Wil2021_COI	GD55	1	BD47702_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47702	Sample	BD47702	targeted	Absent	Sample	Hsan_Wil2021_COI	GD55	COI	45	BD47702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD55	CCTGGGCCGGTATAGTAGGT	151.17706
BD47702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79201	WGS84	BD47702	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47702_Msal_Bot2012_COI	GD55	1	BD47702_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47702	Sample	BD47702	targeted	Absent	Sample	Msal_Bot2012_COI	GD55	COI	45	BD47702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD55	GYTAGTTCCRATGATGTTAGCTG	151.17706
BD47702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79201	WGS84	BD47702	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47702_Pvir_Dia2013_COI	GD55	1	BD47702_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47702	Sample	BD47702	targeted	Absent	Sample	Pvir_Dia2013_COI	GD55	COI	45	BD47702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD55	CTTAGTGGCATTAATTCGDAATCC	151.17706
BD47705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80633	WGS84	BD47705	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47705_Asen_Wil2025_COI	ESAV-172	1	BD47705_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47705	Sample	BD47705	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-172	COI	45	BD47705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-172	GTTGTTGTTACAACACATGCTCTTTTG	138.48422
BD47709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.8328	WGS84	BD47709	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47709_Asen_Wil2025_COI	GC36	1	BD47709_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47709	Sample	BD47709	targeted	Absent	Sample	Asen_Wil2025_COI	GC36	COI	45	BD47709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC36	GTTGTTGTTACAACACATGCTCTTTTG	151.26408
BD47712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82094	WGS84	BD47712	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47712_Asen_Wil2025_COI	GD16	1	BD47712_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47712	Sample	BD47712	targeted	Absent	Sample	Asen_Wil2025_COI	GD16	COI	45	BD47712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD16	GTTGTTGTTACAACACATGCTCTTTTG	151.24059
BD47716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86952	WGS84	BD47716	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47716_Asen_Wil2025_COI	ESAV-115	1	BD47716_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47716	Sample	BD47716	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-115	COI	45	BD47716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-115	GTTGTTGTTACAACACATGCTCTTTTG	138.46521
BD47717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84592	WGS84	BD47717	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47717_Rhar_Sim2018_COI	ESAV-092	1	BD47717_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47717	Sample	BD47717	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-092	COI	45	BD47717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-092	CTGCTGCTATTGCTCATG	138.46728
BD47723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82225	WGS84	BD47723	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47723_Asen_Wil2025_COI	GC43	1	BD47723_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47723	Sample	BD47723	targeted	Absent	Sample	Asen_Wil2025_COI	GC43	COI	45	BD47723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC43	GTTGTTGTTACAACACATGCTCTTTTG	151.24316
BD47724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81567	WGS84	BD47724	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47724_Msal_Bot2012_COI	ESAV-164	1	BD47724_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47724	Sample	BD47724	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-164	COI	45	BD47724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-164	GYTAGTTCCRATGATGTTAGCTG	138.48347
BD47724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81567	WGS84	BD47724	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47724_Rhar_Sim2018_COI	ESAV-164	1	BD47724_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47724	Sample	BD47724	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-164	COI	45	BD47724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-164	CTGCTGCTATTGCTCATG	138.48347
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47727_Asen_Wil2025_COI	GB25	1	BD47727_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Asen_Wil2025_COI	GB25	COI	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	GTTGTTGTTACAACACATGCTCTTTTG	151.26186
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47727_Esin_And2018_Cyb	GB25	1	BD47727_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Esin_And2018_Cyb	GB25	Cytb	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	ACCCCTCCTCATATCCAACCA	151.26186
BD47728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.7899	WGS84	BD47728	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47728_Msal_Bot2012_COI	GC93	1	BD47728_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47728	Sample	BD47728	targeted	Absent	Sample	Msal_Bot2012_COI	GC93	COI	45	BD47728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC93	GYTAGTTCCRATGATGTTAGCTG	151.17787
BD47728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.7899	WGS84	BD47728	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47728_Rhar_Sim2018_COI	GC93	1	BD47728_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47728	Sample	BD47728	targeted	Absent	Sample	Rhar_Sim2018_COI	GC93	COI	45	BD47728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC93	CTGCTGCTATTGCTCATG	151.17787
BD47730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83499	WGS84	BD47730	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47730_Asen_Wil2025_COI	ESAV-057	1	BD47730_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47730	Sample	BD47730	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-057	COI	45	BD47730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-057	GTTGTTGTTACAACACATGCTCTTTTG	138.46238
BD47731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.85136	WGS84	BD47731	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47731_Msal_Bot2012_COI	GC4	1	BD47731_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47731	Sample	BD47731	targeted	Absent	Sample	Msal_Bot2012_COI	GC4	COI	45	BD47731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC4	GYTAGTTCCRATGATGTTAGCTG	151.31385
BD47731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.85136	WGS84	BD47731	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47731_Rhar_Sim2018_COI	GC4	1	BD47731_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47731	Sample	BD47731	targeted	Absent	Sample	Rhar_Sim2018_COI	GC4	COI	45	BD47731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC4	CTGCTGCTATTGCTCATG	151.31385
BD47734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82643	WGS84	BD47734	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47734_Asen_Wil2025_COI	ESAV-152	1	BD47734_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47734	Sample	BD47734	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-152	COI	45	BD47734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-152	GTTGTTGTTACAACACATGCTCTTTTG	138.47756
BD47735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83609	WGS84	BD47735	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47735_Msal_Bot2012_COI	ESAV-143	1	BD47735_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47735	Sample	BD47735	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-143	COI	45	BD47735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-143	GYTAGTTCCRATGATGTTAGCTG	138.4729
BD47735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83609	WGS84	BD47735	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47735_Rhar_Sim2018_COI	ESAV-143	1	BD47735_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47735	Sample	BD47735	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-143	COI	45	BD47735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-143	CTGCTGCTATTGCTCATG	138.4729
BD47737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81579	WGS84	BD47737	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47737_Pcan_Bot2011_IGS	ESAV-080	1	BD47737_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47737	Sample	BD47737	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-080	IGS	45	BD47737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-080	CGTAATCCTCAGTACTGGCTA	138.46367
BD47737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81579	WGS84	BD47737	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47737_Vgib_Wil2025_COI	ESAV-080	1	BD47737_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47737	Sample	BD47737	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-080	COI	45	BD47737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-080	CCCCTCTTTACAAGTTCTTTAGGGT	138.46367
BD47738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.849	WGS84	BD47738	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47738_Asen_Wil2025_COI	ESAV-041	1	BD47738_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47738	Sample	BD47738	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-041	COI	45	BD47738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-041	GTTGTTGTTACAACACATGCTCTTTTG	138.4635
BD47739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.8171	WGS84	BD47739	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47739_Hsan_Wil2021_COI	MA100	1	BD47739_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47739	Sample	BD47739	targeted	Absent	Sample	Hsan_Wil2021_COI	MA100	COI	45	BD47739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA100	CCTGGGCCGGTATAGTAGGT	151.23818
BD47739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.8171	WGS84	BD47739	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47739_Msal_Bot2012_COI	MA100	1	BD47739_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47739	Sample	BD47739	targeted	Absent	Sample	Msal_Bot2012_COI	MA100	COI	45	BD47739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA100	GYTAGTTCCRATGATGTTAGCTG	151.23818
BD47739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.8171	WGS84	BD47739	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47739_Rhar_Sim2018_COI	MA100	1	BD47739_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47739	Sample	BD47739	targeted	Absent	Sample	Rhar_Sim2018_COI	MA100	COI	45	BD47739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA100	CTGCTGCTATTGCTCATG	151.23818
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47740_Pcan_Bot2011_IGS	GB65	1	BD47740_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB65	IGS	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	CGTAATCCTCAGTACTGGCTA	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47740_Pper_Dia2013_COI	GB65	1	BD47740_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Pper_Dia2013_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	CTTAGTGGCATTAATTCGDAATCC	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47740_Vgib_Wil2025_COI	GB65	1	BD47740_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Vgib_Wil2025_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	CCCCTCTTTACAAGTTCTTTAGGGT	151.2453
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47742_Hsan_Wil2021_COI	GB62	1	BD47742_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Hsan_Wil2021_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	CCTGGGCCGGTATAGTAGGT	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47742_Mros_Gib2022_COI	GB62	1	BD47742_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Mros_Gib2022_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47742_Msal_Bot2012_COI	GB62	1	BD47742_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Msal_Bot2012_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	GYTAGTTCCRATGATGTTAGCTG	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47742_Pvir_Dia2013_COI	GB62	1	BD47742_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Pvir_Dia2013_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	CTTAGTGGCATTAATTCGDAATCC	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47742_Rhar_Sim2018_COI	GB62	1	BD47742_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Rhar_Sim2018_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	CTGCTGCTATTGCTCATG	151.178
BD47746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84792	WGS84	BD47746	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47746_Mros_Gib2022_COI	ESAV-136	1	BD47746_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47746	Sample	BD47746	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-136	COI	45	BD47746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-136	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47087
BD47746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84792	WGS84	BD47746	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47746_Pvir_Dia2013_COI	ESAV-136	1	BD47746_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47746	Sample	BD47746	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-136	COI	45	BD47746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-136	CTTAGTGGCATTAATTCGDAATCC	138.47087
BD47749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79231	WGS84	BD47749	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47749_Asen_Wil2025_COI	GC70	1	BD47749_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47749	Sample	BD47749	targeted	Absent	Sample	Asen_Wil2025_COI	GC70	COI	45	BD47749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC70	GTTGTTGTTACAACACATGCTCTTTTG	151.21133
BD47749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79231	WGS84	BD47749	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47749_Esin_And2018_Cyb	GC70	1	BD47749_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47749	Sample	BD47749	targeted	Absent	Sample	Esin_And2018_Cyb	GC70	Cytb	45	BD47749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC70	ACCCCTCCTCATATCCAACCA	151.21133
BD47753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82036	WGS84	BD47753	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47753_Mros_Gib2022_COI	GC54	1	BD47753_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47753	Sample	BD47753	targeted	Absent	Sample	Mros_Gib2022_COI	GC54	COI	45	BD47753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC54	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.24056
BD47753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82036	WGS84	BD47753	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47753_Pvir_Dia2013_COI	GC54	1	BD47753_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47753	Sample	BD47753	targeted	Absent	Sample	Pvir_Dia2013_COI	GC54	COI	45	BD47753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC54	CTTAGTGGCATTAATTCGDAATCC	151.24056
BD47757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81113	WGS84	BD47757	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47757_Mros_Gib2022_COI	ESAV-078	1	BD47757_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47757	Sample	BD47757	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-078	COI	45	BD47757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-078	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46396
BD47757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81113	WGS84	BD47757	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47757_Pvir_Dia2013_COI	ESAV-078	1	BD47757_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47757	Sample	BD47757	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-078	COI	45	BD47757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-078	CTTAGTGGCATTAATTCGDAATCC	138.46396
BD47760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80748	WGS84	BD47760	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47760_Mros_Gib2022_COI	GC108	1	BD47760_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47760	Sample	BD47760	targeted	Absent	Sample	Mros_Gib2022_COI	GC108	COI	45	BD47760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC108	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21661
BD47760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80748	WGS84	BD47760	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47760_Pvir_Dia2013_COI	GC108	1	BD47760_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47760	Sample	BD47760	targeted	Absent	Sample	Pvir_Dia2013_COI	GC108	COI	45	BD47760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC108	CTTAGTGGCATTAATTCGDAATCC	151.21661
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47763_Asen_Wil2025_COI	GB11	1	BD47763_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Asen_Wil2025_COI	GB11	COI	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	GTTGTTGTTACAACACATGCTCTTTTG	151.31153
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47763_Cjap_Sim2018_COI	GB11	1	BD47763_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Cjap_Sim2018_COI	GB11	COI	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31153
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47763_Esin_And2018_Cyb	GB11	1	BD47763_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Esin_And2018_Cyb	GB11	Cytb	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	ACCCCTCCTCATATCCAACCA	151.31153
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47763_Mjap_Gib2022_COI	GB11	1	BD47763_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Mjap_Gib2022_COI	GB11	COI	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	AATTTAATTGTGACTAGGCATGGTCTG	151.31153
BD47764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80057	WGS84	BD47764	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47764_Mros_Gib2022_COI	ESAV-185	1	BD47764_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47764	Sample	BD47764	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-185	COI	45	BD47764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-185	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47727
BD47764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80057	WGS84	BD47764	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47764_Pvir_Dia2013_COI	ESAV-185	1	BD47764_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47764	Sample	BD47764	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-185	COI	45	BD47764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-185	CTTAGTGGCATTAATTCGDAATCC	138.47727
BD47768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86796	WGS84	BD47768	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47768_Mros_Gib2022_COI	ESAV-113	1	BD47768_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47768	Sample	BD47768	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-113	COI	45	BD47768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-113	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46515
BD47768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86796	WGS84	BD47768	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47768_Pvir_Dia2013_COI	ESAV-113	1	BD47768_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47768	Sample	BD47768	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-113	COI	45	BD47768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-113	CTTAGTGGCATTAATTCGDAATCC	138.46515
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47770_Cjap_Sim2018_COI	ESAV-042	1	BD47770_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-042	COI	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46339
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47770_Esin_And2018_Cyb	ESAV-042	1	BD47770_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-042	Cytb	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	ACCCCTCCTCATATCCAACCA	138.46339
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47770_Mjap_Gib2022_COI	ESAV-042	1	BD47770_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-042	COI	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	AATTTAATTGTGACTAGGCATGGTCTG	138.46339
BD47771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82174	WGS84	BD47771	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47771_Mros_Gib2022_COI	MA85	1	BD47771_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47771	Sample	BD47771	targeted	Absent	Sample	Mros_Gib2022_COI	MA85	COI	45	BD47771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA85	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.24289
BD47771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82174	WGS84	BD47771	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47771_Pvir_Dia2013_COI	MA85	1	BD47771_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47771	Sample	BD47771	targeted	Absent	Sample	Pvir_Dia2013_COI	MA85	COI	45	BD47771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA85	CTTAGTGGCATTAATTCGDAATCC	151.24289
BD47775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81663	WGS84	BD47775	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47775_Mros_Gib2022_COI	ESAV-163	1	BD47775_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47775	Sample	BD47775	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-163	COI	45	BD47775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-163	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48299
BD47775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81663	WGS84	BD47775	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47775_Pvir_Dia2013_COI	ESAV-163	1	BD47775_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47775	Sample	BD47775	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-163	COI	45	BD47775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-163	CTTAGTGGCATTAATTCGDAATCC	138.48299
BD47779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83588	WGS84	BD47779	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47779_Mros_Gib2022_COI	GD41	1	BD47779_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47779	Sample	BD47779	targeted	Absent	Sample	Mros_Gib2022_COI	GD41	COI	45	BD47779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD41	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2694
BD47779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83588	WGS84	BD47779	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47779_Pvir_Dia2013_COI	GD41	1	BD47779_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47779	Sample	BD47779	targeted	Absent	Sample	Pvir_Dia2013_COI	GD41	COI	45	BD47779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD41	CTTAGTGGCATTAATTCGDAATCC	151.2694
BD47782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.82483	WGS84	BD47782	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47782_Mros_Gib2022_COI	ESAV-154	1	BD47782_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47782	Sample	BD47782	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-154	COI	45	BD47782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-154	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47925
BD47782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.82483	WGS84	BD47782	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47782_Pvir_Dia2013_COI	ESAV-154	1	BD47782_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47782	Sample	BD47782	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-154	COI	45	BD47782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-154	CTTAGTGGCATTAATTCGDAATCC	138.47925
BD47785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.79862	WGS84	BD47785	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47785_Cjap_Sim2018_COI	ESAV-180	1	BD47785_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47785	Sample	BD47785	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-180	COI	45	BD47785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-180	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48081
BD47789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81762	WGS84	BD47789	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47789_Cjap_Sim2018_COI	ESAV-076	1	BD47789_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47789	Sample	BD47789	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-076	COI	45	BD47789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-076	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46334
BD47792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.78888	WGS84	BD47792	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47792_Cjap_Sim2018_COI	MA50	1	BD47792_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47792	Sample	BD47792	targeted	Absent	Sample	Cjap_Sim2018_COI	MA50	COI	45	BD47792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA50	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17878
BD47796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81754	WGS84	BD47796	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47796_Cjap_Sim2018_COI	ESAV-162	1	BD47796_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47796	Sample	BD47796	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-162	COI	45	BD47796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-162	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48271
BD47800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86882	WGS84	BD47800	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47800_Cjap_Sim2018_COI	ESAV-114	1	BD47800_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47800	Sample	BD47800	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-114	COI	45	BD47800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-114	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46526
BD47804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.96279	WGS84	BD47804	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47804_Cjap_Sim2018_COI	ESAV-237	1	BD47804_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47804	Sample	BD47804	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-237	COI	45	BD47804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-237	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77656
BD47808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.78842	WGS84	BD47808	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47808_Cjap_Sim2018_COI	GC94	1	BD47808_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47808	Sample	BD47808	targeted	Absent	Sample	Cjap_Sim2018_COI	GC94	COI	45	BD47808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC94	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17828
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47809_Mros_Gib2022_COI	GB86	1	BD47809_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Mros_Gib2022_COI	GB86	COI	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.19615
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47809_Msal_Bot2012_COI	GB86	1	BD47809_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Msal_Bot2012_COI	GB86	COI	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	GYTAGTTCCRATGATGTTAGCTG	151.19615
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47809_Pvir_Dia2013_COI	GB86	1	BD47809_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Pvir_Dia2013_COI	GB86	COI	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	CTTAGTGGCATTAATTCGDAATCC	151.19615
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47810_Pcan_Bot2011_IGS	GB41	1	BD47810_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB41	IGS	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	CGTAATCCTCAGTACTGGCTA	151.2175
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47810_Pper_Dia2013_COI	GB41	1	BD47810_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Pper_Dia2013_COI	GB41	COI	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	CTTAGTGGCATTAATTCGDAATCC	151.2175
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47810_Vgib_Wil2025_COI	GB41	1	BD47810_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Vgib_Wil2025_COI	GB41	COI	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	CCCCTCTTTACAAGTTCTTTAGGGT	151.2175
BD47811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.95852	WGS84	BD47811	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47811_Cjap_Sim2018_COI	KPT-A36	1	BD47811_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47811	Sample	BD47811	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A36	COI	45	BD47811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A36	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77848
BD47814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8156	WGS84	BD47814	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47814_Vgib_Wil2025_COI	GB81	1	BD47814_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47814	Sample	BD47814	targeted	Absent	Sample	Vgib_Wil2025_COI	GB81	COI	45	BD47814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB81	CCCCTCTTTACAAGTTCTTTAGGGT	151.23658
BD47815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85421	WGS84	BD47815	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47815_Cjap_Sim2018_COI	GA23	1	BD47815_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47815	Sample	BD47815	targeted	Absent	Sample	Cjap_Sim2018_COI	GA23	COI	45	BD47815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA23	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32248
BD47819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81361	WGS84	BD47819	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47819_Cjap_Sim2018_COI	ESAV-166	1	BD47819_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47819	Sample	BD47819	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-166	COI	45	BD47819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-166	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48392
BD47821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.82491	WGS84	BD47821	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47821_Vgib_Wil2025_COI	ESAV-068	1	BD47821_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47821	Sample	BD47821	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-068	COI	45	BD47821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-068	CCCCTCTTTACAAGTTCTTTAGGGT	138.46246
BD47822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8314	WGS84	BD47822	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47822_Cjap_Sim2018_COI	GD35	1	BD47822_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47822	Sample	BD47822	targeted	Absent	Sample	Cjap_Sim2018_COI	GD35	COI	45	BD47822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD35	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25702
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11307_Asen_Wil2025_COI	CNSA-001	1	BE11307_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	GTTGTTGTTACAACACATGCTCTTTTG	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11307_Cjap_Sim2018_COI	CNSA-001	1	BE11307_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11307_Esin_And2018_Cyb	CNSA-001	1	BE11307_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-001	Cytb	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	ACCCCTCCTCATATCCAACCA	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11307_Mgig_Bot2012_COI	CNSA-001	1	BE11307_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	TCTTATTCGTTGGAGACTTTATAACCCT	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11307_Mjap_Gib2022_COI	CNSA-001	1	BE11307_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	AATTTAATTGTGACTAGGCATGGTCTG	145.77385
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11308_Cmae_Bot2011_COI	CNSA-002	1	BE11308_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	ATGAACAGTCTATCCTCCTTTAG	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11308_Hsan_Wil2021_COI	CNSA-002	1	BE11308_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	CCTGGGCCGGTATAGTAGGT	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11308_Mros_Gib2022_COI	CNSA-002	1	BE11308_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11308_Msal_Bot2012_COI	CNSA-002	1	BE11308_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	GYTAGTTCCRATGATGTTAGCTG	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11308_Mstr_Wil2021_COI	CNSA-002	1	BE11308_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	GTAGTGGTAACTACTCATGCTTTA	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11308_Pvir_Dia2013_COI	CNSA-002	1	BE11308_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	CTTAGTGGCATTAATTCGDAATCC	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11308_Rhar_Sim2018_COI	CNSA-002	1	BE11308_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	CTGCTGCTATTGCTCATG	145.77346
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11310_Asen_Wil2025_COI	CNSA-004	1	BE11310_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	GTTGTTGTTACAACACATGCTCTTTTG	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11310_Cjap_Sim2018_COI	CNSA-004	1	BE11310_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11310_Esin_And2018_Cyb	CNSA-004	1	BE11310_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-004	Cytb	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	ACCCCTCCTCATATCCAACCA	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11310_Mgig_Bot2012_COI	CNSA-004	1	BE11310_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	TCTTATTCGTTGGAGACTTTATAACCCT	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11310_Mjap_Gib2022_COI	CNSA-004	1	BE11310_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	AATTTAATTGTGACTAGGCATGGTCTG	145.77622
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11311_Cmae_Bot2011_COI	CNSA-005	1	BE11311_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	ATGAACAGTCTATCCTCCTTTAG	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11311_Hsan_Wil2021_COI	CNSA-005	1	BE11311_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	CCTGGGCCGGTATAGTAGGT	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11311_Mros_Gib2022_COI	CNSA-005	1	BE11311_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11311_Msal_Bot2012_COI	CNSA-005	1	BE11311_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	GYTAGTTCCRATGATGTTAGCTG	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11311_Mstr_Wil2021_COI	CNSA-005	1	BE11311_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	GTAGTGGTAACTACTCATGCTTTA	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11311_Pvir_Dia2013_COI	CNSA-005	1	BE11311_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	CTTAGTGGCATTAATTCGDAATCC	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11311_Rhar_Sim2018_COI	CNSA-005	1	BE11311_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	CTGCTGCTATTGCTCATG	145.77936
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11313_Aamu_Bax2006_COI	CNSA-007	1	BE11313_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	GCACAACCGGGATCTTTACTTC	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11313_Pcan_Bot2011_IGS	CNSA-007	1	BE11313_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-007	IGS	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	CGTAATCCTCAGTACTGGCTA	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11313_Pper_Dia2013_COI	CNSA-007	1	BE11313_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	CTTAGTGGCATTAATTCGDAATCC	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11313_Sspa_Oph2007_COI	CNSA-007	1	BE11313_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	GAAGGCCTTCCGATGATC	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11313_Upin_Bot2011_COI	CNSA-007	1	BE11313_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	CTTTAATTACAGCGTTTTTATTGTTGT	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11313_Vgib_Wil2025_COI	CNSA-007	1	BE11313_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	CCCCTCTTTACAAGTTCTTTAGGGT	145.7816
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11314_Asen_Wil2025_COI	CNSA-008	1	BE11314_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	GTTGTTGTTACAACACATGCTCTTTTG	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11314_Cjap_Sim2018_COI	CNSA-008	1	BE11314_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11314_Esin_And2018_Cyb	CNSA-008	1	BE11314_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-008	Cytb	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	ACCCCTCCTCATATCCAACCA	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11314_Mgig_Bot2012_COI	CNSA-008	1	BE11314_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	TCTTATTCGTTGGAGACTTTATAACCCT	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11314_Mjap_Gib2022_COI	CNSA-008	1	BE11314_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	AATTTAATTGTGACTAGGCATGGTCTG	145.78272
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11315_Cmae_Bot2011_COI	CNSA-009	1	BE11315_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	ATGAACAGTCTATCCTCCTTTAG	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11315_Hsan_Wil2021_COI	CNSA-009	1	BE11315_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	CCTGGGCCGGTATAGTAGGT	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11315_Mros_Gib2022_COI	CNSA-009	1	BE11315_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11315_Msal_Bot2012_COI	CNSA-009	1	BE11315_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	GYTAGTTCCRATGATGTTAGCTG	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11315_Mstr_Wil2021_COI	CNSA-009	1	BE11315_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	GTAGTGGTAACTACTCATGCTTTA	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11315_Pvir_Dia2013_COI	CNSA-009	1	BE11315_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	CTTAGTGGCATTAATTCGDAATCC	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11315_Rhar_Sim2018_COI	CNSA-009	1	BE11315_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	CTGCTGCTATTGCTCATG	145.78196
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11317_Aamu_Bax2006_COI	CNSA-011	1	BE11317_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	GCACAACCGGGATCTTTACTTC	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11317_Pcan_Bot2011_IGS	CNSA-011	1	BE11317_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-011	IGS	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	CGTAATCCTCAGTACTGGCTA	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11317_Pper_Dia2013_COI	CNSA-011	1	BE11317_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	CTTAGTGGCATTAATTCGDAATCC	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11317_Sspa_Oph2007_COI	CNSA-011	1	BE11317_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	GAAGGCCTTCCGATGATC	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11317_Upin_Bot2011_COI	CNSA-011	1	BE11317_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	CTTTAATTACAGCGTTTTTATTGTTGT	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11317_Vgib_Wil2025_COI	CNSA-011	1	BE11317_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	CCCCTCTTTACAAGTTCTTTAGGGT	145.78089
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11318_Asen_Wil2025_COI	CNSA-012	1	BE11318_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	GTTGTTGTTACAACACATGCTCTTTTG	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11318_Cjap_Sim2018_COI	CNSA-012	1	BE11318_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11318_Esin_And2018_Cyb	CNSA-012	1	BE11318_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-012	Cytb	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	ACCCCTCCTCATATCCAACCA	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11318_Mgig_Bot2012_COI	CNSA-012	1	BE11318_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	TCTTATTCGTTGGAGACTTTATAACCCT	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11318_Mjap_Gib2022_COI	CNSA-012	1	BE11318_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	AATTTAATTGTGACTAGGCATGGTCTG	145.78149
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11319_Cmae_Bot2011_COI	CNSA-013	1	BE11319_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	ATGAACAGTCTATCCTCCTTTAG	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11319_Hsan_Wil2021_COI	CNSA-013	1	BE11319_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	CCTGGGCCGGTATAGTAGGT	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11319_Mros_Gib2022_COI	CNSA-013	1	BE11319_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11319_Msal_Bot2012_COI	CNSA-013	1	BE11319_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	GYTAGTTCCRATGATGTTAGCTG	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11319_Mstr_Wil2021_COI	CNSA-013	1	BE11319_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	GTAGTGGTAACTACTCATGCTTTA	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11319_Pvir_Dia2013_COI	CNSA-013	1	BE11319_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	CTTAGTGGCATTAATTCGDAATCC	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11319_Rhar_Sim2018_COI	CNSA-013	1	BE11319_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	CTGCTGCTATTGCTCATG	145.78071
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11320_Aamu_Bax2006_COI	CNSA-014	1	BE11320_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	GCACAACCGGGATCTTTACTTC	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11320_Pcan_Bot2011_IGS	CNSA-014	1	BE11320_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-014	IGS	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	CGTAATCCTCAGTACTGGCTA	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11320_Pper_Dia2013_COI	CNSA-014	1	BE11320_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	CTTAGTGGCATTAATTCGDAATCC	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11320_Sspa_Oph2007_COI	CNSA-014	1	BE11320_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	GAAGGCCTTCCGATGATC	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11320_Upin_Bot2011_COI	CNSA-014	1	BE11320_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	CTTTAATTACAGCGTTTTTATTGTTGT	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11320_Vgib_Wil2025_COI	CNSA-014	1	BE11320_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	CCCCTCTTTACAAGTTCTTTAGGGT	145.78207
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11321_Asen_Wil2025_COI	CNSA-015	1	BE11321_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	GTTGTTGTTACAACACATGCTCTTTTG	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11321_Cjap_Sim2018_COI	CNSA-015	1	BE11321_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11321_Esin_And2018_Cyb	CNSA-015	1	BE11321_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-015	Cytb	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	ACCCCTCCTCATATCCAACCA	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11321_Mgig_Bot2012_COI	CNSA-015	1	BE11321_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	TCTTATTCGTTGGAGACTTTATAACCCT	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11321_Mjap_Gib2022_COI	CNSA-015	1	BE11321_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	AATTTAATTGTGACTAGGCATGGTCTG	145.78151
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11322_Cmae_Bot2011_COI	CNSA-016	1	BE11322_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	ATGAACAGTCTATCCTCCTTTAG	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11322_Hsan_Wil2021_COI	CNSA-016	1	BE11322_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	CCTGGGCCGGTATAGTAGGT	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11322_Mros_Gib2022_COI	CNSA-016	1	BE11322_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11322_Msal_Bot2012_COI	CNSA-016	1	BE11322_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	GYTAGTTCCRATGATGTTAGCTG	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11322_Mstr_Wil2021_COI	CNSA-016	1	BE11322_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	GTAGTGGTAACTACTCATGCTTTA	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11322_Pvir_Dia2013_COI	CNSA-016	1	BE11322_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	CTTAGTGGCATTAATTCGDAATCC	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11322_Rhar_Sim2018_COI	CNSA-016	1	BE11322_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	CTGCTGCTATTGCTCATG	145.78048
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11324_Aamu_Bax2006_COI	CNSA-018	1	BE11324_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	GCACAACCGGGATCTTTACTTC	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11324_Pcan_Bot2011_IGS	CNSA-018	1	BE11324_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-018	IGS	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	CGTAATCCTCAGTACTGGCTA	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11324_Pper_Dia2013_COI	CNSA-018	1	BE11324_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	CTTAGTGGCATTAATTCGDAATCC	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11324_Sspa_Oph2007_COI	CNSA-018	1	BE11324_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	GAAGGCCTTCCGATGATC	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11324_Upin_Bot2011_COI	CNSA-018	1	BE11324_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	CTTTAATTACAGCGTTTTTATTGTTGT	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11324_Vgib_Wil2025_COI	CNSA-018	1	BE11324_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	CCCCTCTTTACAAGTTCTTTAGGGT	145.78129
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11325_Asen_Wil2025_COI	CNSA-019	1	BE11325_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	GTTGTTGTTACAACACATGCTCTTTTG	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11325_Cjap_Sim2018_COI	CNSA-019	1	BE11325_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11325_Esin_And2018_Cyb	CNSA-019	1	BE11325_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-019	Cytb	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	ACCCCTCCTCATATCCAACCA	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11325_Mgig_Bot2012_COI	CNSA-019	1	BE11325_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	TCTTATTCGTTGGAGACTTTATAACCCT	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11325_Mjap_Gib2022_COI	CNSA-019	1	BE11325_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	AATTTAATTGTGACTAGGCATGGTCTG	145.78272
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11326_Cmae_Bot2011_COI	CNSA-020	1	BE11326_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	ATGAACAGTCTATCCTCCTTTAG	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11326_Hsan_Wil2021_COI	CNSA-020	1	BE11326_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	CCTGGGCCGGTATAGTAGGT	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11326_Mros_Gib2022_COI	CNSA-020	1	BE11326_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11326_Msal_Bot2012_COI	CNSA-020	1	BE11326_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	GYTAGTTCCRATGATGTTAGCTG	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11326_Mstr_Wil2021_COI	CNSA-020	1	BE11326_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	GTAGTGGTAACTACTCATGCTTTA	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11326_Pvir_Dia2013_COI	CNSA-020	1	BE11326_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	CTTAGTGGCATTAATTCGDAATCC	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11326_Rhar_Sim2018_COI	CNSA-020	1	BE11326_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	CTGCTGCTATTGCTCATG	145.78211
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11328_Aamu_Bax2006_COI	CNSA-022	1	BE11328_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	GCACAACCGGGATCTTTACTTC	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11328_Pcan_Bot2011_IGS	CNSA-022	1	BE11328_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-022	IGS	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	CGTAATCCTCAGTACTGGCTA	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11328_Pper_Dia2013_COI	CNSA-022	1	BE11328_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	CTTAGTGGCATTAATTCGDAATCC	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11328_Sspa_Oph2007_COI	CNSA-022	1	BE11328_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	GAAGGCCTTCCGATGATC	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11328_Upin_Bot2011_COI	CNSA-022	1	BE11328_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	CTTTAATTACAGCGTTTTTATTGTTGT	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11328_Vgib_Wil2025_COI	CNSA-022	1	BE11328_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	CCCCTCTTTACAAGTTCTTTAGGGT	145.78172
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11329_Asen_Wil2025_COI	CNSA-023	1	BE11329_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	GTTGTTGTTACAACACATGCTCTTTTG	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11329_Cjap_Sim2018_COI	CNSA-023	1	BE11329_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11329_Esin_And2018_Cyb	CNSA-023	1	BE11329_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-023	Cytb	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	ACCCCTCCTCATATCCAACCA	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11329_Mgig_Bot2012_COI	CNSA-023	1	BE11329_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	TCTTATTCGTTGGAGACTTTATAACCCT	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11329_Mjap_Gib2022_COI	CNSA-023	1	BE11329_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	AATTTAATTGTGACTAGGCATGGTCTG	145.78342
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11331_Aamu_Bax2006_COI	CNSA-025	1	BE11331_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	GCACAACCGGGATCTTTACTTC	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11331_Pcan_Bot2011_IGS	CNSA-025	1	BE11331_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-025	IGS	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	CGTAATCCTCAGTACTGGCTA	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11331_Pper_Dia2013_COI	CNSA-025	1	BE11331_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	CTTAGTGGCATTAATTCGDAATCC	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11331_Sspa_Oph2007_COI	CNSA-025	1	BE11331_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	GAAGGCCTTCCGATGATC	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11331_Upin_Bot2011_COI	CNSA-025	1	BE11331_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	CTTTAATTACAGCGTTTTTATTGTTGT	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11331_Vgib_Wil2025_COI	CNSA-025	1	BE11331_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	CCCCTCTTTACAAGTTCTTTAGGGT	145.78397
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11332_Asen_Wil2025_COI	CNSA-026	1	BE11332_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	GTTGTTGTTACAACACATGCTCTTTTG	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11332_Cjap_Sim2018_COI	CNSA-026	1	BE11332_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11332_Esin_And2018_Cyb	CNSA-026	1	BE11332_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-026	Cytb	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	ACCCCTCCTCATATCCAACCA	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11332_Mgig_Bot2012_COI	CNSA-026	1	BE11332_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	TCTTATTCGTTGGAGACTTTATAACCCT	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11332_Mjap_Gib2022_COI	CNSA-026	1	BE11332_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	AATTTAATTGTGACTAGGCATGGTCTG	145.78469
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11333_Cmae_Bot2011_COI	CNSA-027	1	BE11333_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	ATGAACAGTCTATCCTCCTTTAG	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11333_Hsan_Wil2021_COI	CNSA-027	1	BE11333_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	CCTGGGCCGGTATAGTAGGT	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11333_Mros_Gib2022_COI	CNSA-027	1	BE11333_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11333_Msal_Bot2012_COI	CNSA-027	1	BE11333_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	GYTAGTTCCRATGATGTTAGCTG	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11333_Mstr_Wil2021_COI	CNSA-027	1	BE11333_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	GTAGTGGTAACTACTCATGCTTTA	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11333_Pvir_Dia2013_COI	CNSA-027	1	BE11333_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	CTTAGTGGCATTAATTCGDAATCC	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11333_Rhar_Sim2018_COI	CNSA-027	1	BE11333_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	CTGCTGCTATTGCTCATG	145.78387
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11335_Aamu_Bax2006_COI	CNSA-029	1	BE11335_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	GCACAACCGGGATCTTTACTTC	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11335_Pcan_Bot2011_IGS	CNSA-029	1	BE11335_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-029	IGS	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	CGTAATCCTCAGTACTGGCTA	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11335_Pper_Dia2013_COI	CNSA-029	1	BE11335_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	CTTAGTGGCATTAATTCGDAATCC	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11335_Sspa_Oph2007_COI	CNSA-029	1	BE11335_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	GAAGGCCTTCCGATGATC	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11335_Upin_Bot2011_COI	CNSA-029	1	BE11335_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	CTTTAATTACAGCGTTTTTATTGTTGT	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11335_Vgib_Wil2025_COI	CNSA-029	1	BE11335_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	CCCCTCTTTACAAGTTCTTTAGGGT	145.78451
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11336_Asen_Wil2025_COI	CNSA-030	1	BE11336_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	GTTGTTGTTACAACACATGCTCTTTTG	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11336_Cjap_Sim2018_COI	CNSA-030	1	BE11336_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11336_Esin_And2018_Cyb	CNSA-030	1	BE11336_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-030	Cytb	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	ACCCCTCCTCATATCCAACCA	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11336_Mgig_Bot2012_COI	CNSA-030	1	BE11336_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	TCTTATTCGTTGGAGACTTTATAACCCT	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11336_Mjap_Gib2022_COI	CNSA-030	1	BE11336_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	AATTTAATTGTGACTAGGCATGGTCTG	145.78522
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11337_Cmae_Bot2011_COI	CNSA-031	1	BE11337_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	ATGAACAGTCTATCCTCCTTTAG	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11337_Hsan_Wil2021_COI	CNSA-031	1	BE11337_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	CCTGGGCCGGTATAGTAGGT	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11337_Mros_Gib2022_COI	CNSA-031	1	BE11337_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11337_Msal_Bot2012_COI	CNSA-031	1	BE11337_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	GYTAGTTCCRATGATGTTAGCTG	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11337_Mstr_Wil2021_COI	CNSA-031	1	BE11337_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	GTAGTGGTAACTACTCATGCTTTA	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11337_Pvir_Dia2013_COI	CNSA-031	1	BE11337_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	CTTAGTGGCATTAATTCGDAATCC	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11337_Rhar_Sim2018_COI	CNSA-031	1	BE11337_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	CTGCTGCTATTGCTCATG	145.78462
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11339_Aamu_Bax2006_COI	CNSA-033	1	BE11339_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	GCACAACCGGGATCTTTACTTC	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11339_Pcan_Bot2011_IGS	CNSA-033	1	BE11339_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-033	IGS	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	CGTAATCCTCAGTACTGGCTA	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11339_Pper_Dia2013_COI	CNSA-033	1	BE11339_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	CTTAGTGGCATTAATTCGDAATCC	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11339_Sspa_Oph2007_COI	CNSA-033	1	BE11339_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	GAAGGCCTTCCGATGATC	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11339_Upin_Bot2011_COI	CNSA-033	1	BE11339_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	CTTTAATTACAGCGTTTTTATTGTTGT	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11339_Vgib_Wil2025_COI	CNSA-033	1	BE11339_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	CCCCTCTTTACAAGTTCTTTAGGGT	145.782
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11340_Cmae_Bot2011_COI	CNSA-034	1	BE11340_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	ATGAACAGTCTATCCTCCTTTAG	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11340_Hsan_Wil2021_COI	CNSA-034	1	BE11340_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	CCTGGGCCGGTATAGTAGGT	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11340_Mros_Gib2022_COI	CNSA-034	1	BE11340_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11340_Msal_Bot2012_COI	CNSA-034	1	BE11340_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	GYTAGTTCCRATGATGTTAGCTG	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11340_Mstr_Wil2021_COI	CNSA-034	1	BE11340_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	GTAGTGGTAACTACTCATGCTTTA	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11340_Pvir_Dia2013_COI	CNSA-034	1	BE11340_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	CTTAGTGGCATTAATTCGDAATCC	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11340_Rhar_Sim2018_COI	CNSA-034	1	BE11340_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	CTGCTGCTATTGCTCATG	145.7838
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11346_Aamu_Bax2006_COI	TSVA-001	1	BE11346_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	GCACAACCGGGATCTTTACTTC	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11346_Pcan_Bot2011_IGS	TSVA-001	1	BE11346_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-001	IGS	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	CGTAATCCTCAGTACTGGCTA	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11346_Pper_Dia2013_COI	TSVA-001	1	BE11346_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	CTTAGTGGCATTAATTCGDAATCC	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11346_Sspa_Oph2007_COI	TSVA-001	1	BE11346_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	GAAGGCCTTCCGATGATC	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11346_Upin_Bot2011_COI	TSVA-001	1	BE11346_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	CTTTAATTACAGCGTTTTTATTGTTGT	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11346_Vgib_Wil2025_COI	TSVA-001	1	BE11346_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	CCCCTCTTTACAAGTTCTTTAGGGT	146.8425
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11347_Asen_Wil2025_COI	TSVA-002	1	BE11347_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	GTTGTTGTTACAACACATGCTCTTTTG	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11347_Cjap_Sim2018_COI	TSVA-002	1	BE11347_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11347_Esin_And2018_Cyb	TSVA-002	1	BE11347_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-002	Cytb	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	ACCCCTCCTCATATCCAACCA	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11347_Mgig_Bot2012_COI	TSVA-002	1	BE11347_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	TCTTATTCGTTGGAGACTTTATAACCCT	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11347_Mjap_Gib2022_COI	TSVA-002	1	BE11347_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	AATTTAATTGTGACTAGGCATGGTCTG	146.84366
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11348_Cmae_Bot2011_COI	TSVA-003	1	BE11348_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	ATGAACAGTCTATCCTCCTTTAG	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11348_Hsan_Wil2021_COI	TSVA-003	1	BE11348_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	CCTGGGCCGGTATAGTAGGT	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11348_Mros_Gib2022_COI	TSVA-003	1	BE11348_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11348_Msal_Bot2012_COI	TSVA-003	1	BE11348_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	GYTAGTTCCRATGATGTTAGCTG	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11348_Mstr_Wil2021_COI	TSVA-003	1	BE11348_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	GTAGTGGTAACTACTCATGCTTTA	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11348_Pvir_Dia2013_COI	TSVA-003	1	BE11348_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	CTTAGTGGCATTAATTCGDAATCC	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11348_Rhar_Sim2018_COI	TSVA-003	1	BE11348_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	CTGCTGCTATTGCTCATG	146.8419
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11350_Asen_Wil2025_COI	TSVA-005	1	BE11350_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	GTTGTTGTTACAACACATGCTCTTTTG	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11350_Cjap_Sim2018_COI	TSVA-005	1	BE11350_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11350_Esin_And2018_Cyb	TSVA-005	1	BE11350_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-005	Cytb	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	ACCCCTCCTCATATCCAACCA	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11350_Mgig_Bot2012_COI	TSVA-005	1	BE11350_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	TCTTATTCGTTGGAGACTTTATAACCCT	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11350_Mjap_Gib2022_COI	TSVA-005	1	BE11350_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	AATTTAATTGTGACTAGGCATGGTCTG	146.84022
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11351_Cmae_Bot2011_COI	TSVA-006	1	BE11351_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	ATGAACAGTCTATCCTCCTTTAG	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11351_Hsan_Wil2021_COI	TSVA-006	1	BE11351_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	CCTGGGCCGGTATAGTAGGT	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11351_Mros_Gib2022_COI	TSVA-006	1	BE11351_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11351_Msal_Bot2012_COI	TSVA-006	1	BE11351_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	GYTAGTTCCRATGATGTTAGCTG	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11351_Mstr_Wil2021_COI	TSVA-006	1	BE11351_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	GTAGTGGTAACTACTCATGCTTTA	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11351_Pvir_Dia2013_COI	TSVA-006	1	BE11351_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	CTTAGTGGCATTAATTCGDAATCC	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11351_Rhar_Sim2018_COI	TSVA-006	1	BE11351_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	CTGCTGCTATTGCTCATG	146.83958
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11353_Aamu_Bax2006_COI	TSVA-008	1	BE11353_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	GCACAACCGGGATCTTTACTTC	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11353_Pcan_Bot2011_IGS	TSVA-008	1	BE11353_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-008	IGS	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	CGTAATCCTCAGTACTGGCTA	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11353_Pper_Dia2013_COI	TSVA-008	1	BE11353_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	CTTAGTGGCATTAATTCGDAATCC	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11353_Sspa_Oph2007_COI	TSVA-008	1	BE11353_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	GAAGGCCTTCCGATGATC	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11353_Upin_Bot2011_COI	TSVA-008	1	BE11353_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	CTTTAATTACAGCGTTTTTATTGTTGT	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11353_Vgib_Wil2025_COI	TSVA-008	1	BE11353_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	CCCCTCTTTACAAGTTCTTTAGGGT	146.83795
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11354_Asen_Wil2025_COI	TSVA-009	1	BE11354_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	GTTGTTGTTACAACACATGCTCTTTTG	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11354_Cjap_Sim2018_COI	TSVA-009	1	BE11354_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11354_Esin_And2018_Cyb	TSVA-009	1	BE11354_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-009	Cytb	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	ACCCCTCCTCATATCCAACCA	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11354_Mgig_Bot2012_COI	TSVA-009	1	BE11354_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	TCTTATTCGTTGGAGACTTTATAACCCT	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11354_Mjap_Gib2022_COI	TSVA-009	1	BE11354_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	AATTTAATTGTGACTAGGCATGGTCTG	146.83964
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11355_Cmae_Bot2011_COI	TSVA-010	1	BE11355_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	ATGAACAGTCTATCCTCCTTTAG	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11355_Hsan_Wil2021_COI	TSVA-010	1	BE11355_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	CCTGGGCCGGTATAGTAGGT	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11355_Mros_Gib2022_COI	TSVA-010	1	BE11355_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11355_Msal_Bot2012_COI	TSVA-010	1	BE11355_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	GYTAGTTCCRATGATGTTAGCTG	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11355_Mstr_Wil2021_COI	TSVA-010	1	BE11355_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	GTAGTGGTAACTACTCATGCTTTA	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11355_Pvir_Dia2013_COI	TSVA-010	1	BE11355_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	CTTAGTGGCATTAATTCGDAATCC	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11355_Rhar_Sim2018_COI	TSVA-010	1	BE11355_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	CTGCTGCTATTGCTCATG	146.84064
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11357_Aamu_Bax2006_COI	TSVA-012	1	BE11357_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	GCACAACCGGGATCTTTACTTC	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11357_Pcan_Bot2011_IGS	TSVA-012	1	BE11357_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-012	IGS	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	CGTAATCCTCAGTACTGGCTA	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11357_Pper_Dia2013_COI	TSVA-012	1	BE11357_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	CTTAGTGGCATTAATTCGDAATCC	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11357_Sspa_Oph2007_COI	TSVA-012	1	BE11357_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	GAAGGCCTTCCGATGATC	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11357_Upin_Bot2011_COI	TSVA-012	1	BE11357_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	CTTTAATTACAGCGTTTTTATTGTTGT	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11357_Vgib_Wil2025_COI	TSVA-012	1	BE11357_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	CCCCTCTTTACAAGTTCTTTAGGGT	146.83955
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11358_Asen_Wil2025_COI	TSVA-013	1	BE11358_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	GTTGTTGTTACAACACATGCTCTTTTG	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11358_Cjap_Sim2018_COI	TSVA-013	1	BE11358_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11358_Esin_And2018_Cyb	TSVA-013	1	BE11358_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-013	Cytb	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	ACCCCTCCTCATATCCAACCA	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11358_Mgig_Bot2012_COI	TSVA-013	1	BE11358_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	TCTTATTCGTTGGAGACTTTATAACCCT	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11358_Mjap_Gib2022_COI	TSVA-013	1	BE11358_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	AATTTAATTGTGACTAGGCATGGTCTG	146.83785
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11359_Cmae_Bot2011_COI	TSVA-014	1	BE11359_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	ATGAACAGTCTATCCTCCTTTAG	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11359_Hsan_Wil2021_COI	TSVA-014	1	BE11359_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	CCTGGGCCGGTATAGTAGGT	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11359_Mros_Gib2022_COI	TSVA-014	1	BE11359_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11359_Msal_Bot2012_COI	TSVA-014	1	BE11359_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	GYTAGTTCCRATGATGTTAGCTG	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11359_Mstr_Wil2021_COI	TSVA-014	1	BE11359_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	GTAGTGGTAACTACTCATGCTTTA	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11359_Pvir_Dia2013_COI	TSVA-014	1	BE11359_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	CTTAGTGGCATTAATTCGDAATCC	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11359_Rhar_Sim2018_COI	TSVA-014	1	BE11359_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	CTGCTGCTATTGCTCATG	146.83647
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11360_Aamu_Bax2006_COI	TSVA-015	1	BE11360_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	GCACAACCGGGATCTTTACTTC	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11360_Pcan_Bot2011_IGS	TSVA-015	1	BE11360_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-015	IGS	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	CGTAATCCTCAGTACTGGCTA	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11360_Pper_Dia2013_COI	TSVA-015	1	BE11360_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	CTTAGTGGCATTAATTCGDAATCC	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11360_Sspa_Oph2007_COI	TSVA-015	1	BE11360_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	GAAGGCCTTCCGATGATC	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11360_Upin_Bot2011_COI	TSVA-015	1	BE11360_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	CTTTAATTACAGCGTTTTTATTGTTGT	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11360_Vgib_Wil2025_COI	TSVA-015	1	BE11360_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	CCCCTCTTTACAAGTTCTTTAGGGT	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11361_Asen_Wil2025_COI	TSVA-016	1	BE11361_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	GTTGTTGTTACAACACATGCTCTTTTG	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11361_Cjap_Sim2018_COI	TSVA-016	1	BE11361_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11361_Esin_And2018_Cyb	TSVA-016	1	BE11361_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-016	Cytb	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	ACCCCTCCTCATATCCAACCA	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11361_Mgig_Bot2012_COI	TSVA-016	1	BE11361_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	TCTTATTCGTTGGAGACTTTATAACCCT	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11361_Mjap_Gib2022_COI	TSVA-016	1	BE11361_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	AATTTAATTGTGACTAGGCATGGTCTG	146.83612
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11362_Cmae_Bot2011_COI	TSVA-017	1	BE11362_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	ATGAACAGTCTATCCTCCTTTAG	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11362_Hsan_Wil2021_COI	TSVA-017	1	BE11362_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	CCTGGGCCGGTATAGTAGGT	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11362_Mros_Gib2022_COI	TSVA-017	1	BE11362_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11362_Msal_Bot2012_COI	TSVA-017	1	BE11362_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	GYTAGTTCCRATGATGTTAGCTG	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11362_Mstr_Wil2021_COI	TSVA-017	1	BE11362_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	GTAGTGGTAACTACTCATGCTTTA	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11362_Pvir_Dia2013_COI	TSVA-017	1	BE11362_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	CTTAGTGGCATTAATTCGDAATCC	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11362_Rhar_Sim2018_COI	TSVA-017	1	BE11362_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	CTGCTGCTATTGCTCATG	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11364_Aamu_Bax2006_COI	TSVA-019	1	BE11364_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	GCACAACCGGGATCTTTACTTC	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11364_Pcan_Bot2011_IGS	TSVA-019	1	BE11364_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-019	IGS	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	CGTAATCCTCAGTACTGGCTA	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11364_Pper_Dia2013_COI	TSVA-019	1	BE11364_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	CTTAGTGGCATTAATTCGDAATCC	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11364_Sspa_Oph2007_COI	TSVA-019	1	BE11364_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	GAAGGCCTTCCGATGATC	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11364_Upin_Bot2011_COI	TSVA-019	1	BE11364_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	CTTTAATTACAGCGTTTTTATTGTTGT	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11364_Vgib_Wil2025_COI	TSVA-019	1	BE11364_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	CCCCTCTTTACAAGTTCTTTAGGGT	146.83385
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11365_Asen_Wil2025_COI	TSVA-020	1	BE11365_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	GTTGTTGTTACAACACATGCTCTTTTG	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11365_Cjap_Sim2018_COI	TSVA-020	1	BE11365_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11365_Esin_And2018_Cyb	TSVA-020	1	BE11365_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-020	Cytb	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	ACCCCTCCTCATATCCAACCA	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11365_Mgig_Bot2012_COI	TSVA-020	1	BE11365_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	TCTTATTCGTTGGAGACTTTATAACCCT	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11365_Mjap_Gib2022_COI	TSVA-020	1	BE11365_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	AATTTAATTGTGACTAGGCATGGTCTG	146.83638
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11366_Cmae_Bot2011_COI	TSVA-021	1	BE11366_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	ATGAACAGTCTATCCTCCTTTAG	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11366_Hsan_Wil2021_COI	TSVA-021	1	BE11366_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	CCTGGGCCGGTATAGTAGGT	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11366_Mros_Gib2022_COI	TSVA-021	1	BE11366_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11366_Msal_Bot2012_COI	TSVA-021	1	BE11366_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	GYTAGTTCCRATGATGTTAGCTG	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11366_Mstr_Wil2021_COI	TSVA-021	1	BE11366_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	GTAGTGGTAACTACTCATGCTTTA	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11366_Pvir_Dia2013_COI	TSVA-021	1	BE11366_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	CTTAGTGGCATTAATTCGDAATCC	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11366_Rhar_Sim2018_COI	TSVA-021	1	BE11366_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	CTGCTGCTATTGCTCATG	146.83773
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11368_Aamu_Bax2006_COI	TSVA-023	1	BE11368_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	GCACAACCGGGATCTTTACTTC	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11368_Pcan_Bot2011_IGS	TSVA-023	1	BE11368_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-023	IGS	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	CGTAATCCTCAGTACTGGCTA	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11368_Pper_Dia2013_COI	TSVA-023	1	BE11368_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	CTTAGTGGCATTAATTCGDAATCC	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11368_Sspa_Oph2007_COI	TSVA-023	1	BE11368_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	GAAGGCCTTCCGATGATC	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11368_Upin_Bot2011_COI	TSVA-023	1	BE11368_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	CTTTAATTACAGCGTTTTTATTGTTGT	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11368_Vgib_Wil2025_COI	TSVA-023	1	BE11368_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	CCCCTCTTTACAAGTTCTTTAGGGT	146.83503
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11369_Asen_Wil2025_COI	TSVA-024	1	BE11369_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	GTTGTTGTTACAACACATGCTCTTTTG	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11369_Cjap_Sim2018_COI	TSVA-024	1	BE11369_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11369_Esin_And2018_Cyb	TSVA-024	1	BE11369_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-024	Cytb	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	ACCCCTCCTCATATCCAACCA	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11369_Mgig_Bot2012_COI	TSVA-024	1	BE11369_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	TCTTATTCGTTGGAGACTTTATAACCCT	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11369_Mjap_Gib2022_COI	TSVA-024	1	BE11369_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	AATTTAATTGTGACTAGGCATGGTCTG	146.835
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11371_Aamu_Bax2006_COI	TSVA-026	1	BE11371_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	GCACAACCGGGATCTTTACTTC	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11371_Pcan_Bot2011_IGS	TSVA-026	1	BE11371_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-026	IGS	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	CGTAATCCTCAGTACTGGCTA	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11371_Pper_Dia2013_COI	TSVA-026	1	BE11371_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	CTTAGTGGCATTAATTCGDAATCC	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11371_Sspa_Oph2007_COI	TSVA-026	1	BE11371_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	GAAGGCCTTCCGATGATC	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11371_Upin_Bot2011_COI	TSVA-026	1	BE11371_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	CTTTAATTACAGCGTTTTTATTGTTGT	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11371_Vgib_Wil2025_COI	TSVA-026	1	BE11371_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	CCCCTCTTTACAAGTTCTTTAGGGT	146.83212
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11372_Asen_Wil2025_COI	TSVA-027	1	BE11372_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	GTTGTTGTTACAACACATGCTCTTTTG	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11372_Cjap_Sim2018_COI	TSVA-027	1	BE11372_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11372_Esin_And2018_Cyb	TSVA-027	1	BE11372_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-027	Cytb	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	ACCCCTCCTCATATCCAACCA	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11372_Mgig_Bot2012_COI	TSVA-027	1	BE11372_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	TCTTATTCGTTGGAGACTTTATAACCCT	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11372_Mjap_Gib2022_COI	TSVA-027	1	BE11372_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	AATTTAATTGTGACTAGGCATGGTCTG	146.83145
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11373_Cmae_Bot2011_COI	TSVA-028	1	BE11373_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	ATGAACAGTCTATCCTCCTTTAG	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11373_Hsan_Wil2021_COI	TSVA-028	1	BE11373_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	CCTGGGCCGGTATAGTAGGT	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11373_Mros_Gib2022_COI	TSVA-028	1	BE11373_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11373_Msal_Bot2012_COI	TSVA-028	1	BE11373_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	GYTAGTTCCRATGATGTTAGCTG	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11373_Mstr_Wil2021_COI	TSVA-028	1	BE11373_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	GTAGTGGTAACTACTCATGCTTTA	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11373_Pvir_Dia2013_COI	TSVA-028	1	BE11373_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	CTTAGTGGCATTAATTCGDAATCC	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11373_Rhar_Sim2018_COI	TSVA-028	1	BE11373_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	CTGCTGCTATTGCTCATG	146.83453
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11375_Aamu_Bax2006_COI	TSVA-030	1	BE11375_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	GCACAACCGGGATCTTTACTTC	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11375_Pcan_Bot2011_IGS	TSVA-030	1	BE11375_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-030	IGS	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	CGTAATCCTCAGTACTGGCTA	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11375_Pper_Dia2013_COI	TSVA-030	1	BE11375_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	CTTAGTGGCATTAATTCGDAATCC	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11375_Sspa_Oph2007_COI	TSVA-030	1	BE11375_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	GAAGGCCTTCCGATGATC	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11375_Upin_Bot2011_COI	TSVA-030	1	BE11375_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	CTTTAATTACAGCGTTTTTATTGTTGT	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11375_Vgib_Wil2025_COI	TSVA-030	1	BE11375_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	CCCCTCTTTACAAGTTCTTTAGGGT	146.83475
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11376_Asen_Wil2025_COI	TSVA-031	1	BE11376_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	GTTGTTGTTACAACACATGCTCTTTTG	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11376_Cjap_Sim2018_COI	TSVA-031	1	BE11376_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11376_Esin_And2018_Cyb	TSVA-031	1	BE11376_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-031	Cytb	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	ACCCCTCCTCATATCCAACCA	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11376_Mgig_Bot2012_COI	TSVA-031	1	BE11376_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	TCTTATTCGTTGGAGACTTTATAACCCT	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11376_Mjap_Gib2022_COI	TSVA-031	1	BE11376_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	AATTTAATTGTGACTAGGCATGGTCTG	146.83336
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11377_Cmae_Bot2011_COI	TSVA-032	1	BE11377_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	ATGAACAGTCTATCCTCCTTTAG	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11377_Hsan_Wil2021_COI	TSVA-032	1	BE11377_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	CCTGGGCCGGTATAGTAGGT	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11377_Mros_Gib2022_COI	TSVA-032	1	BE11377_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11377_Msal_Bot2012_COI	TSVA-032	1	BE11377_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	GYTAGTTCCRATGATGTTAGCTG	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11377_Mstr_Wil2021_COI	TSVA-032	1	BE11377_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	GTAGTGGTAACTACTCATGCTTTA	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11377_Pvir_Dia2013_COI	TSVA-032	1	BE11377_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	CTTAGTGGCATTAATTCGDAATCC	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11377_Rhar_Sim2018_COI	TSVA-032	1	BE11377_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	CTGCTGCTATTGCTCATG	146.83341
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11379_Aamu_Bax2006_COI	TSVA-034	1	BE11379_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	GCACAACCGGGATCTTTACTTC	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11379_Pcan_Bot2011_IGS	TSVA-034	1	BE11379_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-034	IGS	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	CGTAATCCTCAGTACTGGCTA	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11379_Pper_Dia2013_COI	TSVA-034	1	BE11379_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	CTTAGTGGCATTAATTCGDAATCC	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11379_Sspa_Oph2007_COI	TSVA-034	1	BE11379_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	GAAGGCCTTCCGATGATC	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11379_Upin_Bot2011_COI	TSVA-034	1	BE11379_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	CTTTAATTACAGCGTTTTTATTGTTGT	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11379_Vgib_Wil2025_COI	TSVA-034	1	BE11379_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	CCCCTCTTTACAAGTTCTTTAGGGT	146.83376
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11380_Cmae_Bot2011_COI	TSVA-035	1	BE11380_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	ATGAACAGTCTATCCTCCTTTAG	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11380_Hsan_Wil2021_COI	TSVA-035	1	BE11380_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	CCTGGGCCGGTATAGTAGGT	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11380_Mros_Gib2022_COI	TSVA-035	1	BE11380_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11380_Msal_Bot2012_COI	TSVA-035	1	BE11380_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	GYTAGTTCCRATGATGTTAGCTG	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11380_Mstr_Wil2021_COI	TSVA-035	1	BE11380_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	GTAGTGGTAACTACTCATGCTTTA	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11380_Pvir_Dia2013_COI	TSVA-035	1	BE11380_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	CTTAGTGGCATTAATTCGDAATCC	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11380_Rhar_Sim2018_COI	TSVA-035	1	BE11380_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	CTGCTGCTATTGCTCATG	146.83379
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11386_Aamu_Bax2006_COI	GLTA-002	1	BE11386_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	GCACAACCGGGATCTTTACTTC	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11386_Pcan_Bot2011_IGS	GLTA-002	1	BE11386_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-002	IGS	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	CGTAATCCTCAGTACTGGCTA	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11386_Pper_Dia2013_COI	GLTA-002	1	BE11386_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	CTTAGTGGCATTAATTCGDAATCC	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11386_Sspa_Oph2007_COI	GLTA-002	1	BE11386_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	GAAGGCCTTCCGATGATC	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11386_Upin_Bot2011_COI	GLTA-002	1	BE11386_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	CTTTAATTACAGCGTTTTTATTGTTGT	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11386_Vgib_Wil2025_COI	GLTA-002	1	BE11386_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	CCCCTCTTTACAAGTTCTTTAGGGT	151.32328
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11387_Asen_Wil2025_COI	GLTA-003	1	BE11387_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	GTTGTTGTTACAACACATGCTCTTTTG	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11387_Cjap_Sim2018_COI	GLTA-003	1	BE11387_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11387_Esin_And2018_Cyb	GLTA-003	1	BE11387_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-003	Cytb	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	ACCCCTCCTCATATCCAACCA	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11387_Mgig_Bot2012_COI	GLTA-003	1	BE11387_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	TCTTATTCGTTGGAGACTTTATAACCCT	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11387_Mjap_Gib2022_COI	GLTA-003	1	BE11387_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	AATTTAATTGTGACTAGGCATGGTCTG	151.32533
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11388_Cmae_Bot2011_COI	GLTA-004	1	BE11388_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	ATGAACAGTCTATCCTCCTTTAG	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11388_Hsan_Wil2021_COI	GLTA-004	1	BE11388_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	CCTGGGCCGGTATAGTAGGT	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11388_Mros_Gib2022_COI	GLTA-004	1	BE11388_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11388_Msal_Bot2012_COI	GLTA-004	1	BE11388_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	GYTAGTTCCRATGATGTTAGCTG	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11388_Mstr_Wil2021_COI	GLTA-004	1	BE11388_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	GTAGTGGTAACTACTCATGCTTTA	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11388_Pvir_Dia2013_COI	GLTA-004	1	BE11388_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	CTTAGTGGCATTAATTCGDAATCC	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11388_Rhar_Sim2018_COI	GLTA-004	1	BE11388_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	CTGCTGCTATTGCTCATG	151.32448
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11390_Asen_Wil2025_COI	GLTA-006	1	BE11390_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	GTTGTTGTTACAACACATGCTCTTTTG	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11390_Cjap_Sim2018_COI	GLTA-006	1	BE11390_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11390_Esin_And2018_Cyb	GLTA-006	1	BE11390_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-006	Cytb	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	ACCCCTCCTCATATCCAACCA	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11390_Mgig_Bot2012_COI	GLTA-006	1	BE11390_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	TCTTATTCGTTGGAGACTTTATAACCCT	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11390_Mjap_Gib2022_COI	GLTA-006	1	BE11390_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	AATTTAATTGTGACTAGGCATGGTCTG	151.32283
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11391_Cmae_Bot2011_COI	GLTA-007	1	BE11391_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	ATGAACAGTCTATCCTCCTTTAG	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11391_Hsan_Wil2021_COI	GLTA-007	1	BE11391_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	CCTGGGCCGGTATAGTAGGT	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11391_Mros_Gib2022_COI	GLTA-007	1	BE11391_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11391_Msal_Bot2012_COI	GLTA-007	1	BE11391_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	GYTAGTTCCRATGATGTTAGCTG	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11391_Mstr_Wil2021_COI	GLTA-007	1	BE11391_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	GTAGTGGTAACTACTCATGCTTTA	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11391_Pvir_Dia2013_COI	GLTA-007	1	BE11391_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	CTTAGTGGCATTAATTCGDAATCC	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11391_Rhar_Sim2018_COI	GLTA-007	1	BE11391_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	CTGCTGCTATTGCTCATG	151.32495
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11393_Aamu_Bax2006_COI	GLTA-009	1	BE11393_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	GCACAACCGGGATCTTTACTTC	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11393_Pcan_Bot2011_IGS	GLTA-009	1	BE11393_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-009	IGS	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	CGTAATCCTCAGTACTGGCTA	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11393_Pper_Dia2013_COI	GLTA-009	1	BE11393_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	CTTAGTGGCATTAATTCGDAATCC	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11393_Sspa_Oph2007_COI	GLTA-009	1	BE11393_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	GAAGGCCTTCCGATGATC	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11393_Upin_Bot2011_COI	GLTA-009	1	BE11393_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	CTTTAATTACAGCGTTTTTATTGTTGT	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11393_Vgib_Wil2025_COI	GLTA-009	1	BE11393_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	CCCCTCTTTACAAGTTCTTTAGGGT	151.31842
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11394_Asen_Wil2025_COI	GLTA-010	1	BE11394_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	GTTGTTGTTACAACACATGCTCTTTTG	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11394_Cjap_Sim2018_COI	GLTA-010	1	BE11394_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11394_Esin_And2018_Cyb	GLTA-010	1	BE11394_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-010	Cytb	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	ACCCCTCCTCATATCCAACCA	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11394_Mgig_Bot2012_COI	GLTA-010	1	BE11394_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	TCTTATTCGTTGGAGACTTTATAACCCT	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11394_Mjap_Gib2022_COI	GLTA-010	1	BE11394_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	AATTTAATTGTGACTAGGCATGGTCTG	151.32062
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11395_Cmae_Bot2011_COI	GLTA-011	1	BE11395_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	ATGAACAGTCTATCCTCCTTTAG	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11395_Hsan_Wil2021_COI	GLTA-011	1	BE11395_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	CCTGGGCCGGTATAGTAGGT	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11395_Mros_Gib2022_COI	GLTA-011	1	BE11395_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11395_Msal_Bot2012_COI	GLTA-011	1	BE11395_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	GYTAGTTCCRATGATGTTAGCTG	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11395_Mstr_Wil2021_COI	GLTA-011	1	BE11395_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	GTAGTGGTAACTACTCATGCTTTA	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11395_Pvir_Dia2013_COI	GLTA-011	1	BE11395_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	CTTAGTGGCATTAATTCGDAATCC	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11395_Rhar_Sim2018_COI	GLTA-011	1	BE11395_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	CTGCTGCTATTGCTCATG	151.32214
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11397_Aamu_Bax2006_COI	GLTA-013	1	BE11397_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	GCACAACCGGGATCTTTACTTC	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11397_Pcan_Bot2011_IGS	GLTA-013	1	BE11397_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-013	IGS	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	CGTAATCCTCAGTACTGGCTA	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11397_Pper_Dia2013_COI	GLTA-013	1	BE11397_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	CTTAGTGGCATTAATTCGDAATCC	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11397_Sspa_Oph2007_COI	GLTA-013	1	BE11397_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	GAAGGCCTTCCGATGATC	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11397_Upin_Bot2011_COI	GLTA-013	1	BE11397_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	CTTTAATTACAGCGTTTTTATTGTTGT	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11397_Vgib_Wil2025_COI	GLTA-013	1	BE11397_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	CCCCTCTTTACAAGTTCTTTAGGGT	151.3073
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11398_Asen_Wil2025_COI	GLTA-014	1	BE11398_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	GTTGTTGTTACAACACATGCTCTTTTG	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11398_Cjap_Sim2018_COI	GLTA-014	1	BE11398_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11398_Esin_And2018_Cyb	GLTA-014	1	BE11398_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-014	Cytb	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	ACCCCTCCTCATATCCAACCA	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11398_Mgig_Bot2012_COI	GLTA-014	1	BE11398_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	TCTTATTCGTTGGAGACTTTATAACCCT	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11398_Mjap_Gib2022_COI	GLTA-014	1	BE11398_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	AATTTAATTGTGACTAGGCATGGTCTG	151.31563
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11399_Cmae_Bot2011_COI	GLTA-015	1	BE11399_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	ATGAACAGTCTATCCTCCTTTAG	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11399_Hsan_Wil2021_COI	GLTA-015	1	BE11399_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	CCTGGGCCGGTATAGTAGGT	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11399_Mros_Gib2022_COI	GLTA-015	1	BE11399_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11399_Msal_Bot2012_COI	GLTA-015	1	BE11399_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	GYTAGTTCCRATGATGTTAGCTG	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11399_Mstr_Wil2021_COI	GLTA-015	1	BE11399_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	GTAGTGGTAACTACTCATGCTTTA	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11399_Pvir_Dia2013_COI	GLTA-015	1	BE11399_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	CTTAGTGGCATTAATTCGDAATCC	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11399_Rhar_Sim2018_COI	GLTA-015	1	BE11399_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	CTGCTGCTATTGCTCATG	151.31768
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11401_Aamu_Bax2006_COI	GLTA-017	1	BE11401_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	GCACAACCGGGATCTTTACTTC	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11401_Pcan_Bot2011_IGS	GLTA-017	1	BE11401_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-017	IGS	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	CGTAATCCTCAGTACTGGCTA	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11401_Pper_Dia2013_COI	GLTA-017	1	BE11401_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	CTTAGTGGCATTAATTCGDAATCC	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11401_Sspa_Oph2007_COI	GLTA-017	1	BE11401_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	GAAGGCCTTCCGATGATC	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11401_Upin_Bot2011_COI	GLTA-017	1	BE11401_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	CTTTAATTACAGCGTTTTTATTGTTGT	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11401_Vgib_Wil2025_COI	GLTA-017	1	BE11401_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	CCCCTCTTTACAAGTTCTTTAGGGT	151.3209
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11402_Asen_Wil2025_COI	GLTA-018	1	BE11402_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	GTTGTTGTTACAACACATGCTCTTTTG	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11402_Cjap_Sim2018_COI	GLTA-018	1	BE11402_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11402_Esin_And2018_Cyb	GLTA-018	1	BE11402_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-018	Cytb	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	ACCCCTCCTCATATCCAACCA	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11402_Mgig_Bot2012_COI	GLTA-018	1	BE11402_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	TCTTATTCGTTGGAGACTTTATAACCCT	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11402_Mjap_Gib2022_COI	GLTA-018	1	BE11402_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	AATTTAATTGTGACTAGGCATGGTCTG	151.32283
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11403_Cmae_Bot2011_COI	GLTA-019	1	BE11403_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	ATGAACAGTCTATCCTCCTTTAG	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11403_Hsan_Wil2021_COI	GLTA-019	1	BE11403_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	CCTGGGCCGGTATAGTAGGT	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11403_Mros_Gib2022_COI	GLTA-019	1	BE11403_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11403_Msal_Bot2012_COI	GLTA-019	1	BE11403_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	GYTAGTTCCRATGATGTTAGCTG	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11403_Mstr_Wil2021_COI	GLTA-019	1	BE11403_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	GTAGTGGTAACTACTCATGCTTTA	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11403_Pvir_Dia2013_COI	GLTA-019	1	BE11403_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	CTTAGTGGCATTAATTCGDAATCC	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11403_Rhar_Sim2018_COI	GLTA-019	1	BE11403_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	CTGCTGCTATTGCTCATG	151.30614
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11405_Aamu_Bax2006_COI	GLTA-021	1	BE11405_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	GCACAACCGGGATCTTTACTTC	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11405_Pcan_Bot2011_IGS	GLTA-021	1	BE11405_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-021	IGS	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	CGTAATCCTCAGTACTGGCTA	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11405_Pper_Dia2013_COI	GLTA-021	1	BE11405_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	CTTAGTGGCATTAATTCGDAATCC	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11405_Sspa_Oph2007_COI	GLTA-021	1	BE11405_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	GAAGGCCTTCCGATGATC	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11405_Upin_Bot2011_COI	GLTA-021	1	BE11405_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	CTTTAATTACAGCGTTTTTATTGTTGT	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11405_Vgib_Wil2025_COI	GLTA-021	1	BE11405_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	CCCCTCTTTACAAGTTCTTTAGGGT	151.30947
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11406_Asen_Wil2025_COI	GLTA-022	1	BE11406_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	GTTGTTGTTACAACACATGCTCTTTTG	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11406_Cjap_Sim2018_COI	GLTA-022	1	BE11406_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11406_Esin_And2018_Cyb	GLTA-022	1	BE11406_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-022	Cytb	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	ACCCCTCCTCATATCCAACCA	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11406_Mgig_Bot2012_COI	GLTA-022	1	BE11406_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	TCTTATTCGTTGGAGACTTTATAACCCT	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11406_Mjap_Gib2022_COI	GLTA-022	1	BE11406_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	AATTTAATTGTGACTAGGCATGGTCTG	151.31087
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11407_Cmae_Bot2011_COI	GLTA-023	1	BE11407_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	ATGAACAGTCTATCCTCCTTTAG	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11407_Hsan_Wil2021_COI	GLTA-023	1	BE11407_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	CCTGGGCCGGTATAGTAGGT	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11407_Mros_Gib2022_COI	GLTA-023	1	BE11407_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11407_Msal_Bot2012_COI	GLTA-023	1	BE11407_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	GYTAGTTCCRATGATGTTAGCTG	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11407_Mstr_Wil2021_COI	GLTA-023	1	BE11407_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	GTAGTGGTAACTACTCATGCTTTA	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11407_Pvir_Dia2013_COI	GLTA-023	1	BE11407_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	CTTAGTGGCATTAATTCGDAATCC	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11407_Rhar_Sim2018_COI	GLTA-023	1	BE11407_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	CTGCTGCTATTGCTCATG	151.31293
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11409_Aamu_Bax2006_COI	GLTA-025	1	BE11409_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	GCACAACCGGGATCTTTACTTC	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11409_Pcan_Bot2011_IGS	GLTA-025	1	BE11409_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-025	IGS	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	CGTAATCCTCAGTACTGGCTA	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11409_Pper_Dia2013_COI	GLTA-025	1	BE11409_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	CTTAGTGGCATTAATTCGDAATCC	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11409_Sspa_Oph2007_COI	GLTA-025	1	BE11409_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	GAAGGCCTTCCGATGATC	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11409_Upin_Bot2011_COI	GLTA-025	1	BE11409_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	CTTTAATTACAGCGTTTTTATTGTTGT	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11409_Vgib_Wil2025_COI	GLTA-025	1	BE11409_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	CCCCTCTTTACAAGTTCTTTAGGGT	151.3163
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11410_Cmae_Bot2011_COI	GLTA-026	1	BE11410_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	ATGAACAGTCTATCCTCCTTTAG	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11410_Hsan_Wil2021_COI	GLTA-026	1	BE11410_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	CCTGGGCCGGTATAGTAGGT	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11410_Mros_Gib2022_COI	GLTA-026	1	BE11410_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11410_Msal_Bot2012_COI	GLTA-026	1	BE11410_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	GYTAGTTCCRATGATGTTAGCTG	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11410_Mstr_Wil2021_COI	GLTA-026	1	BE11410_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	GTAGTGGTAACTACTCATGCTTTA	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11410_Pvir_Dia2013_COI	GLTA-026	1	BE11410_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	CTTAGTGGCATTAATTCGDAATCC	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11410_Rhar_Sim2018_COI	GLTA-026	1	BE11410_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	CTGCTGCTATTGCTCATG	151.31826
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11412_Aamu_Bax2006_COI	GLTA-028	1	BE11412_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	GCACAACCGGGATCTTTACTTC	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11412_Pcan_Bot2011_IGS	GLTA-028	1	BE11412_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-028	IGS	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	CGTAATCCTCAGTACTGGCTA	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11412_Pper_Dia2013_COI	GLTA-028	1	BE11412_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	CTTAGTGGCATTAATTCGDAATCC	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11412_Sspa_Oph2007_COI	GLTA-028	1	BE11412_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	GAAGGCCTTCCGATGATC	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11412_Upin_Bot2011_COI	GLTA-028	1	BE11412_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	CTTTAATTACAGCGTTTTTATTGTTGT	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11412_Vgib_Wil2025_COI	GLTA-028	1	BE11412_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	CCCCTCTTTACAAGTTCTTTAGGGT	151.30704
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11413_Asen_Wil2025_COI	GLTA-029	1	BE11413_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	GTTGTTGTTACAACACATGCTCTTTTG	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11413_Cjap_Sim2018_COI	GLTA-029	1	BE11413_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11413_Esin_And2018_Cyb	GLTA-029	1	BE11413_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-029	Cytb	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	ACCCCTCCTCATATCCAACCA	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11413_Mgig_Bot2012_COI	GLTA-029	1	BE11413_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	TCTTATTCGTTGGAGACTTTATAACCCT	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11413_Mjap_Gib2022_COI	GLTA-029	1	BE11413_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	AATTTAATTGTGACTAGGCATGGTCTG	151.3087
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11414_Cmae_Bot2011_COI	GLTA-030	1	BE11414_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	ATGAACAGTCTATCCTCCTTTAG	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11414_Hsan_Wil2021_COI	GLTA-030	1	BE11414_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	CCTGGGCCGGTATAGTAGGT	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11414_Mros_Gib2022_COI	GLTA-030	1	BE11414_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11414_Msal_Bot2012_COI	GLTA-030	1	BE11414_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	GYTAGTTCCRATGATGTTAGCTG	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11414_Mstr_Wil2021_COI	GLTA-030	1	BE11414_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	GTAGTGGTAACTACTCATGCTTTA	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11414_Pvir_Dia2013_COI	GLTA-030	1	BE11414_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	CTTAGTGGCATTAATTCGDAATCC	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11414_Rhar_Sim2018_COI	GLTA-030	1	BE11414_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	CTGCTGCTATTGCTCATG	151.31051
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11416_Aamu_Bax2006_COI	GLTA-032	1	BE11416_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	GCACAACCGGGATCTTTACTTC	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11416_Pcan_Bot2011_IGS	GLTA-032	1	BE11416_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-032	IGS	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	CGTAATCCTCAGTACTGGCTA	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11416_Pper_Dia2013_COI	GLTA-032	1	BE11416_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	CTTAGTGGCATTAATTCGDAATCC	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11416_Sspa_Oph2007_COI	GLTA-032	1	BE11416_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	GAAGGCCTTCCGATGATC	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11416_Upin_Bot2011_COI	GLTA-032	1	BE11416_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	CTTTAATTACAGCGTTTTTATTGTTGT	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11416_Vgib_Wil2025_COI	GLTA-032	1	BE11416_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	CCCCTCTTTACAAGTTCTTTAGGGT	151.31387
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11417_Asen_Wil2025_COI	GLTA-033	1	BE11417_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	GTTGTTGTTACAACACATGCTCTTTTG	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11417_Cjap_Sim2018_COI	GLTA-033	1	BE11417_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11417_Esin_And2018_Cyb	GLTA-033	1	BE11417_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-033	Cytb	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	ACCCCTCCTCATATCCAACCA	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11417_Mgig_Bot2012_COI	GLTA-033	1	BE11417_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	TCTTATTCGTTGGAGACTTTATAACCCT	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11417_Mjap_Gib2022_COI	GLTA-033	1	BE11417_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	AATTTAATTGTGACTAGGCATGGTCTG	151.3157
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11418_Cmae_Bot2011_COI	GLTA-034	1	BE11418_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	ATGAACAGTCTATCCTCCTTTAG	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11418_Mros_Gib2022_COI	GLTA-034	1	BE11418_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11418_Msal_Bot2012_COI	GLTA-034	1	BE11418_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	GYTAGTTCCRATGATGTTAGCTG	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11418_Mstr_Wil2021_COI	GLTA-034	1	BE11418_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	GTAGTGGTAACTACTCATGCTTTA	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11418_Pvir_Dia2013_COI	GLTA-034	1	BE11418_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	CTTAGTGGCATTAATTCGDAATCC	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11418_Rhar_Sim2018_COI	GLTA-034	1	BE11418_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	CTGCTGCTATTGCTCATG	151.31106
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11424_Asen_Wil2025_COI	GLTA-036	1	BE11424_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	GTTGTTGTTACAACACATGCTCTTTTG	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11424_Cjap_Sim2018_COI	GLTA-036	1	BE11424_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11424_Esin_And2018_Cyb	GLTA-036	1	BE11424_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-036	Cytb	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	ACCCCTCCTCATATCCAACCA	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11424_Mgig_Bot2012_COI	GLTA-036	1	BE11424_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	TCTTATTCGTTGGAGACTTTATAACCCT	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11424_Mjap_Gib2022_COI	GLTA-036	1	BE11424_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	AATTTAATTGTGACTAGGCATGGTCTG	151.27517
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11425_Cmae_Bot2011_COI	GLTA-037	1	BE11425_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	ATGAACAGTCTATCCTCCTTTAG	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11425_Hsan_Wil2021_COI	GLTA-037	1	BE11425_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	CCTGGGCCGGTATAGTAGGT	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11425_Mros_Gib2022_COI	GLTA-037	1	BE11425_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11425_Msal_Bot2012_COI	GLTA-037	1	BE11425_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	GYTAGTTCCRATGATGTTAGCTG	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11425_Mstr_Wil2021_COI	GLTA-037	1	BE11425_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	GTAGTGGTAACTACTCATGCTTTA	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11425_Pvir_Dia2013_COI	GLTA-037	1	BE11425_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	CTTAGTGGCATTAATTCGDAATCC	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11425_Rhar_Sim2018_COI	GLTA-037	1	BE11425_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	CTGCTGCTATTGCTCATG	151.27401
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11427_Aamu_Bax2006_COI	GLTA-039	1	BE11427_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	GCACAACCGGGATCTTTACTTC	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11427_Pcan_Bot2011_IGS	GLTA-039	1	BE11427_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-039	IGS	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	CGTAATCCTCAGTACTGGCTA	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11427_Pper_Dia2013_COI	GLTA-039	1	BE11427_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	CTTAGTGGCATTAATTCGDAATCC	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11427_Sspa_Oph2007_COI	GLTA-039	1	BE11427_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	GAAGGCCTTCCGATGATC	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11427_Upin_Bot2011_COI	GLTA-039	1	BE11427_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	CTTTAATTACAGCGTTTTTATTGTTGT	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11427_Vgib_Wil2025_COI	GLTA-039	1	BE11427_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	CCCCTCTTTACAAGTTCTTTAGGGT	151.27652
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11428_Asen_Wil2025_COI	GLTA-040	1	BE11428_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	GTTGTTGTTACAACACATGCTCTTTTG	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11428_Cjap_Sim2018_COI	GLTA-040	1	BE11428_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11428_Esin_And2018_Cyb	GLTA-040	1	BE11428_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-040	Cytb	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	ACCCCTCCTCATATCCAACCA	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11428_Mgig_Bot2012_COI	GLTA-040	1	BE11428_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	TCTTATTCGTTGGAGACTTTATAACCCT	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11428_Mjap_Gib2022_COI	GLTA-040	1	BE11428_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	AATTTAATTGTGACTAGGCATGGTCTG	151.2752
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11429_Cmae_Bot2011_COI	GLTA-041	1	BE11429_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	ATGAACAGTCTATCCTCCTTTAG	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11429_Hsan_Wil2021_COI	GLTA-041	1	BE11429_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	CCTGGGCCGGTATAGTAGGT	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11429_Mros_Gib2022_COI	GLTA-041	1	BE11429_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11429_Msal_Bot2012_COI	GLTA-041	1	BE11429_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	GYTAGTTCCRATGATGTTAGCTG	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11429_Mstr_Wil2021_COI	GLTA-041	1	BE11429_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	GTAGTGGTAACTACTCATGCTTTA	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11429_Pvir_Dia2013_COI	GLTA-041	1	BE11429_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	CTTAGTGGCATTAATTCGDAATCC	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11429_Rhar_Sim2018_COI	GLTA-041	1	BE11429_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	CTGCTGCTATTGCTCATG	151.27704
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11430_Aamu_Bax2006_COI	GLTA-042	1	BE11430_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	GCACAACCGGGATCTTTACTTC	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11430_Pcan_Bot2011_IGS	GLTA-042	1	BE11430_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-042	IGS	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	CGTAATCCTCAGTACTGGCTA	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11430_Pper_Dia2013_COI	GLTA-042	1	BE11430_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	CTTAGTGGCATTAATTCGDAATCC	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11430_Sspa_Oph2007_COI	GLTA-042	1	BE11430_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	GAAGGCCTTCCGATGATC	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11430_Upin_Bot2011_COI	GLTA-042	1	BE11430_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	CTTTAATTACAGCGTTTTTATTGTTGT	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11430_Vgib_Wil2025_COI	GLTA-042	1	BE11430_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	CCCCTCTTTACAAGTTCTTTAGGGT	151.26694
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11431_Asen_Wil2025_COI	GLTA-043	1	BE11431_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	GTTGTTGTTACAACACATGCTCTTTTG	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11431_Cjap_Sim2018_COI	GLTA-043	1	BE11431_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11431_Esin_And2018_Cyb	GLTA-043	1	BE11431_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-043	Cytb	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	ACCCCTCCTCATATCCAACCA	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11431_Mgig_Bot2012_COI	GLTA-043	1	BE11431_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	TCTTATTCGTTGGAGACTTTATAACCCT	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11431_Mjap_Gib2022_COI	GLTA-043	1	BE11431_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	AATTTAATTGTGACTAGGCATGGTCTG	151.26895
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11432_Cmae_Bot2011_COI	GLTA-044	1	BE11432_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	ATGAACAGTCTATCCTCCTTTAG	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11432_Hsan_Wil2021_COI	GLTA-044	1	BE11432_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	CCTGGGCCGGTATAGTAGGT	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11432_Mros_Gib2022_COI	GLTA-044	1	BE11432_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11432_Msal_Bot2012_COI	GLTA-044	1	BE11432_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	GYTAGTTCCRATGATGTTAGCTG	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11432_Mstr_Wil2021_COI	GLTA-044	1	BE11432_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	GTAGTGGTAACTACTCATGCTTTA	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11432_Pvir_Dia2013_COI	GLTA-044	1	BE11432_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	CTTAGTGGCATTAATTCGDAATCC	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11432_Rhar_Sim2018_COI	GLTA-044	1	BE11432_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	CTGCTGCTATTGCTCATG	151.27076
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11434_Aamu_Bax2006_COI	GLTA-046	1	BE11434_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	GCACAACCGGGATCTTTACTTC	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11434_Pcan_Bot2011_IGS	GLTA-046	1	BE11434_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-046	IGS	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	CGTAATCCTCAGTACTGGCTA	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11434_Pper_Dia2013_COI	GLTA-046	1	BE11434_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	CTTAGTGGCATTAATTCGDAATCC	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11434_Sspa_Oph2007_COI	GLTA-046	1	BE11434_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	GAAGGCCTTCCGATGATC	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11434_Upin_Bot2011_COI	GLTA-046	1	BE11434_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	CTTTAATTACAGCGTTTTTATTGTTGT	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11434_Vgib_Wil2025_COI	GLTA-046	1	BE11434_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	CCCCTCTTTACAAGTTCTTTAGGGT	151.27417
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11435_Asen_Wil2025_COI	GLTA-047	1	BE11435_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	GTTGTTGTTACAACACATGCTCTTTTG	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11435_Cjap_Sim2018_COI	GLTA-047	1	BE11435_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11435_Esin_And2018_Cyb	GLTA-047	1	BE11435_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-047	Cytb	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	ACCCCTCCTCATATCCAACCA	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11435_Mgig_Bot2012_COI	GLTA-047	1	BE11435_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	TCTTATTCGTTGGAGACTTTATAACCCT	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11435_Mjap_Gib2022_COI	GLTA-047	1	BE11435_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	AATTTAATTGTGACTAGGCATGGTCTG	151.27718
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11436_Cmae_Bot2011_COI	GLTA-048	1	BE11436_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	ATGAACAGTCTATCCTCCTTTAG	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11436_Hsan_Wil2021_COI	GLTA-048	1	BE11436_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	CCTGGGCCGGTATAGTAGGT	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11436_Mros_Gib2022_COI	GLTA-048	1	BE11436_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11436_Msal_Bot2012_COI	GLTA-048	1	BE11436_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	GYTAGTTCCRATGATGTTAGCTG	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11436_Mstr_Wil2021_COI	GLTA-048	1	BE11436_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	GTAGTGGTAACTACTCATGCTTTA	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11436_Pvir_Dia2013_COI	GLTA-048	1	BE11436_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	CTTAGTGGCATTAATTCGDAATCC	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11436_Rhar_Sim2018_COI	GLTA-048	1	BE11436_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	CTGCTGCTATTGCTCATG	151.26603
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11438_Aamu_Bax2006_COI	GLTA-050	1	BE11438_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	GCACAACCGGGATCTTTACTTC	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11438_Pcan_Bot2011_IGS	GLTA-050	1	BE11438_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-050	IGS	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	CGTAATCCTCAGTACTGGCTA	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11438_Pper_Dia2013_COI	GLTA-050	1	BE11438_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	CTTAGTGGCATTAATTCGDAATCC	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11438_Sspa_Oph2007_COI	GLTA-050	1	BE11438_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	GAAGGCCTTCCGATGATC	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11438_Upin_Bot2011_COI	GLTA-050	1	BE11438_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	CTTTAATTACAGCGTTTTTATTGTTGT	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11438_Vgib_Wil2025_COI	GLTA-050	1	BE11438_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	CCCCTCTTTACAAGTTCTTTAGGGT	151.26955
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11439_Asen_Wil2025_COI	GLTA-051	1	BE11439_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	GTTGTTGTTACAACACATGCTCTTTTG	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11439_Cjap_Sim2018_COI	GLTA-051	1	BE11439_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11439_Esin_And2018_Cyb	GLTA-051	1	BE11439_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-051	Cytb	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	ACCCCTCCTCATATCCAACCA	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11439_Mgig_Bot2012_COI	GLTA-051	1	BE11439_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	TCTTATTCGTTGGAGACTTTATAACCCT	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11439_Mjap_Gib2022_COI	GLTA-051	1	BE11439_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	AATTTAATTGTGACTAGGCATGGTCTG	151.27186
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11441_Aamu_Bax2006_COI	GLTA-053	1	BE11441_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	GCACAACCGGGATCTTTACTTC	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11441_Pcan_Bot2011_IGS	GLTA-053	1	BE11441_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-053	IGS	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	CGTAATCCTCAGTACTGGCTA	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11441_Pper_Dia2013_COI	GLTA-053	1	BE11441_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	CTTAGTGGCATTAATTCGDAATCC	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11441_Sspa_Oph2007_COI	GLTA-053	1	BE11441_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	GAAGGCCTTCCGATGATC	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11441_Upin_Bot2011_COI	GLTA-053	1	BE11441_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	CTTTAATTACAGCGTTTTTATTGTTGT	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11441_Vgib_Wil2025_COI	GLTA-053	1	BE11441_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	CCCCTCTTTACAAGTTCTTTAGGGT	151.26337
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11442_Asen_Wil2025_COI	GLTA-054	1	BE11442_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	GTTGTTGTTACAACACATGCTCTTTTG	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11442_Cjap_Sim2018_COI	GLTA-054	1	BE11442_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11442_Esin_And2018_Cyb	GLTA-054	1	BE11442_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-054	Cytb	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	ACCCCTCCTCATATCCAACCA	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11442_Mgig_Bot2012_COI	GLTA-054	1	BE11442_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	TCTTATTCGTTGGAGACTTTATAACCCT	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11442_Mjap_Gib2022_COI	GLTA-054	1	BE11442_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	AATTTAATTGTGACTAGGCATGGTCTG	151.26466
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11443_Cmae_Bot2011_COI	GLTA-055	1	BE11443_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	ATGAACAGTCTATCCTCCTTTAG	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11443_Hsan_Wil2021_COI	GLTA-055	1	BE11443_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	CCTGGGCCGGTATAGTAGGT	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11443_Mros_Gib2022_COI	GLTA-055	1	BE11443_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11443_Msal_Bot2012_COI	GLTA-055	1	BE11443_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	GYTAGTTCCRATGATGTTAGCTG	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11443_Mstr_Wil2021_COI	GLTA-055	1	BE11443_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	GTAGTGGTAACTACTCATGCTTTA	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11443_Pvir_Dia2013_COI	GLTA-055	1	BE11443_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	CTTAGTGGCATTAATTCGDAATCC	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11443_Rhar_Sim2018_COI	GLTA-055	1	BE11443_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	CTGCTGCTATTGCTCATG	151.26634
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11445_Aamu_Bax2006_COI	GLTA-057	1	BE11445_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	GCACAACCGGGATCTTTACTTC	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11445_Pcan_Bot2011_IGS	GLTA-057	1	BE11445_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-057	IGS	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	CGTAATCCTCAGTACTGGCTA	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11445_Pper_Dia2013_COI	GLTA-057	1	BE11445_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	CTTAGTGGCATTAATTCGDAATCC	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11445_Sspa_Oph2007_COI	GLTA-057	1	BE11445_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	GAAGGCCTTCCGATGATC	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11445_Upin_Bot2011_COI	GLTA-057	1	BE11445_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	CTTTAATTACAGCGTTTTTATTGTTGT	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11445_Vgib_Wil2025_COI	GLTA-057	1	BE11445_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	CCCCTCTTTACAAGTTCTTTAGGGT	151.25797
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11446_Asen_Wil2025_COI	GLTA-058	1	BE11446_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	GTTGTTGTTACAACACATGCTCTTTTG	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11446_Cjap_Sim2018_COI	GLTA-058	1	BE11446_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11446_Esin_And2018_Cyb	GLTA-058	1	BE11446_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-058	Cytb	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	ACCCCTCCTCATATCCAACCA	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11446_Mgig_Bot2012_COI	GLTA-058	1	BE11446_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	TCTTATTCGTTGGAGACTTTATAACCCT	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11446_Mjap_Gib2022_COI	GLTA-058	1	BE11446_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	AATTTAATTGTGACTAGGCATGGTCTG	151.25936
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11447_Cmae_Bot2011_COI	GLTA-059	1	BE11447_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	ATGAACAGTCTATCCTCCTTTAG	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11447_Hsan_Wil2021_COI	GLTA-059	1	BE11447_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	CCTGGGCCGGTATAGTAGGT	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11447_Mros_Gib2022_COI	GLTA-059	1	BE11447_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11447_Msal_Bot2012_COI	GLTA-059	1	BE11447_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	GYTAGTTCCRATGATGTTAGCTG	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11447_Mstr_Wil2021_COI	GLTA-059	1	BE11447_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	GTAGTGGTAACTACTCATGCTTTA	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11447_Pvir_Dia2013_COI	GLTA-059	1	BE11447_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	CTTAGTGGCATTAATTCGDAATCC	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11447_Rhar_Sim2018_COI	GLTA-059	1	BE11447_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	CTGCTGCTATTGCTCATG	151.26129
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11449_Aamu_Bax2006_COI	GLTA-061	1	BE11449_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	GCACAACCGGGATCTTTACTTC	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11449_Pcan_Bot2011_IGS	GLTA-061	1	BE11449_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-061	IGS	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	CGTAATCCTCAGTACTGGCTA	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11449_Pper_Dia2013_COI	GLTA-061	1	BE11449_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	CTTAGTGGCATTAATTCGDAATCC	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11449_Sspa_Oph2007_COI	GLTA-061	1	BE11449_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	GAAGGCCTTCCGATGATC	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11449_Upin_Bot2011_COI	GLTA-061	1	BE11449_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	CTTTAATTACAGCGTTTTTATTGTTGT	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11449_Vgib_Wil2025_COI	GLTA-061	1	BE11449_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	CCCCTCTTTACAAGTTCTTTAGGGT	151.26484
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11450_Cmae_Bot2011_COI	GLTA-062	1	BE11450_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	ATGAACAGTCTATCCTCCTTTAG	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11450_Hsan_Wil2021_COI	GLTA-062	1	BE11450_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	CCTGGGCCGGTATAGTAGGT	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11450_Mros_Gib2022_COI	GLTA-062	1	BE11450_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11450_Msal_Bot2012_COI	GLTA-062	1	BE11450_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	GYTAGTTCCRATGATGTTAGCTG	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11450_Mstr_Wil2021_COI	GLTA-062	1	BE11450_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	GTAGTGGTAACTACTCATGCTTTA	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11450_Pvir_Dia2013_COI	GLTA-062	1	BE11450_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	CTTAGTGGCATTAATTCGDAATCC	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11450_Rhar_Sim2018_COI	GLTA-062	1	BE11450_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	CTGCTGCTATTGCTCATG	151.25286
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11452_Aamu_Bax2006_COI	GLTA-064	1	BE11452_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	GCACAACCGGGATCTTTACTTC	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11452_Pcan_Bot2011_IGS	GLTA-064	1	BE11452_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-064	IGS	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	CGTAATCCTCAGTACTGGCTA	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11452_Pper_Dia2013_COI	GLTA-064	1	BE11452_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	CTTAGTGGCATTAATTCGDAATCC	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11452_Sspa_Oph2007_COI	GLTA-064	1	BE11452_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	GAAGGCCTTCCGATGATC	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11452_Upin_Bot2011_COI	GLTA-064	1	BE11452_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	CTTTAATTACAGCGTTTTTATTGTTGT	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11452_Vgib_Wil2025_COI	GLTA-064	1	BE11452_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	CCCCTCTTTACAAGTTCTTTAGGGT	151.25594
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11453_Asen_Wil2025_COI	GLTA-065	1	BE11453_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	GTTGTTGTTACAACACATGCTCTTTTG	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11453_Cjap_Sim2018_COI	GLTA-065	1	BE11453_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11453_Esin_And2018_Cyb	GLTA-065	1	BE11453_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-065	Cytb	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	ACCCCTCCTCATATCCAACCA	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11453_Mgig_Bot2012_COI	GLTA-065	1	BE11453_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	TCTTATTCGTTGGAGACTTTATAACCCT	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11453_Mjap_Gib2022_COI	GLTA-065	1	BE11453_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	AATTTAATTGTGACTAGGCATGGTCTG	151.25786
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11454_Cmae_Bot2011_COI	GLTA-066	1	BE11454_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	ATGAACAGTCTATCCTCCTTTAG	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11454_Hsan_Wil2021_COI	GLTA-066	1	BE11454_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	CCTGGGCCGGTATAGTAGGT	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11454_Mros_Gib2022_COI	GLTA-066	1	BE11454_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11454_Msal_Bot2012_COI	GLTA-066	1	BE11454_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	GYTAGTTCCRATGATGTTAGCTG	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11454_Mstr_Wil2021_COI	GLTA-066	1	BE11454_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	GTAGTGGTAACTACTCATGCTTTA	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11454_Pvir_Dia2013_COI	GLTA-066	1	BE11454_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	CTTAGTGGCATTAATTCGDAATCC	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11454_Rhar_Sim2018_COI	GLTA-066	1	BE11454_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	CTGCTGCTATTGCTCATG	151.26022
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11456_Aamu_Bax2006_COI	GLTA-068	1	BE11456_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	GCACAACCGGGATCTTTACTTC	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11456_Pcan_Bot2011_IGS	GLTA-068	1	BE11456_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-068	IGS	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	CGTAATCCTCAGTACTGGCTA	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11456_Pper_Dia2013_COI	GLTA-068	1	BE11456_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	CTTAGTGGCATTAATTCGDAATCC	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11456_Sspa_Oph2007_COI	GLTA-068	1	BE11456_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	GAAGGCCTTCCGATGATC	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11456_Upin_Bot2011_COI	GLTA-068	1	BE11456_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	CTTTAATTACAGCGTTTTTATTGTTGT	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11456_Vgib_Wil2025_COI	GLTA-068	1	BE11456_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	CCCCTCTTTACAAGTTCTTTAGGGT	151.25335
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11457_Asen_Wil2025_COI	GLTA-069	1	BE11457_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	GTTGTTGTTACAACACATGCTCTTTTG	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11457_Cjap_Sim2018_COI	GLTA-069	1	BE11457_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11457_Esin_And2018_Cyb	GLTA-069	1	BE11457_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-069	Cytb	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	ACCCCTCCTCATATCCAACCA	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11457_Mgig_Bot2012_COI	GLTA-069	1	BE11457_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	TCTTATTCGTTGGAGACTTTATAACCCT	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11457_Mjap_Gib2022_COI	GLTA-069	1	BE11457_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	AATTTAATTGTGACTAGGCATGGTCTG	151.25516
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11458_Cmae_Bot2011_COI	GLTA-070	1	BE11458_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	ATGAACAGTCTATCCTCCTTTAG	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11458_Hsan_Wil2021_COI	GLTA-070	1	BE11458_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	CCTGGGCCGGTATAGTAGGT	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11458_Mros_Gib2022_COI	GLTA-070	1	BE11458_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11458_Msal_Bot2012_COI	GLTA-070	1	BE11458_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	GYTAGTTCCRATGATGTTAGCTG	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11458_Mstr_Wil2021_COI	GLTA-070	1	BE11458_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	GTAGTGGTAACTACTCATGCTTTA	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11458_Pvir_Dia2013_COI	GLTA-070	1	BE11458_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	CTTAGTGGCATTAATTCGDAATCC	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11458_Rhar_Sim2018_COI	GLTA-070	1	BE11458_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	CTGCTGCTATTGCTCATG	151.25701
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11463_Aamu_Bax2006_COI	GLTA-076	1	BE11463_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	GCACAACCGGGATCTTTACTTC	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11463_Pcan_Bot2011_IGS	GLTA-076	1	BE11463_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-076	IGS	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	CGTAATCCTCAGTACTGGCTA	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11463_Pper_Dia2013_COI	GLTA-076	1	BE11463_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	CTTAGTGGCATTAATTCGDAATCC	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11463_Sspa_Oph2007_COI	GLTA-076	1	BE11463_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	GAAGGCCTTCCGATGATC	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11463_Upin_Bot2011_COI	GLTA-076	1	BE11463_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	CTTTAATTACAGCGTTTTTATTGTTGT	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11463_Vgib_Wil2025_COI	GLTA-076	1	BE11463_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	CCCCTCTTTACAAGTTCTTTAGGGT	151.18532
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11464_Asen_Wil2025_COI	GLTA-077	1	BE11464_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	GTTGTTGTTACAACACATGCTCTTTTG	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11464_Cjap_Sim2018_COI	GLTA-077	1	BE11464_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11464_Esin_And2018_Cyb	GLTA-077	1	BE11464_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-077	Cytb	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	ACCCCTCCTCATATCCAACCA	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11464_Mgig_Bot2012_COI	GLTA-077	1	BE11464_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	TCTTATTCGTTGGAGACTTTATAACCCT	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11464_Mjap_Gib2022_COI	GLTA-077	1	BE11464_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	AATTTAATTGTGACTAGGCATGGTCTG	151.18717
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11465_Cmae_Bot2011_COI	GLTA-078	1	BE11465_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	ATGAACAGTCTATCCTCCTTTAG	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11465_Hsan_Wil2021_COI	GLTA-078	1	BE11465_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	CCTGGGCCGGTATAGTAGGT	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11465_Mros_Gib2022_COI	GLTA-078	1	BE11465_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11465_Msal_Bot2012_COI	GLTA-078	1	BE11465_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	GYTAGTTCCRATGATGTTAGCTG	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11465_Mstr_Wil2021_COI	GLTA-078	1	BE11465_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	GTAGTGGTAACTACTCATGCTTTA	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11465_Pvir_Dia2013_COI	GLTA-078	1	BE11465_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	CTTAGTGGCATTAATTCGDAATCC	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11465_Rhar_Sim2018_COI	GLTA-078	1	BE11465_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	CTGCTGCTATTGCTCATG	151.18864
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11467_Aamu_Bax2006_COI	GLTA-080	1	BE11467_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	GCACAACCGGGATCTTTACTTC	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11467_Pcan_Bot2011_IGS	GLTA-080	1	BE11467_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-080	IGS	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	CGTAATCCTCAGTACTGGCTA	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11467_Pper_Dia2013_COI	GLTA-080	1	BE11467_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	CTTAGTGGCATTAATTCGDAATCC	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11467_Sspa_Oph2007_COI	GLTA-080	1	BE11467_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	GAAGGCCTTCCGATGATC	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11467_Upin_Bot2011_COI	GLTA-080	1	BE11467_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	CTTTAATTACAGCGTTTTTATTGTTGT	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11467_Vgib_Wil2025_COI	GLTA-080	1	BE11467_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	CCCCTCTTTACAAGTTCTTTAGGGT	151.18602
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11468_Asen_Wil2025_COI	GLTA-081	1	BE11468_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	GTTGTTGTTACAACACATGCTCTTTTG	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11468_Cjap_Sim2018_COI	GLTA-081	1	BE11468_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11468_Esin_And2018_Cyb	GLTA-081	1	BE11468_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-081	Cytb	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	ACCCCTCCTCATATCCAACCA	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11468_Mgig_Bot2012_COI	GLTA-081	1	BE11468_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	TCTTATTCGTTGGAGACTTTATAACCCT	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11468_Mjap_Gib2022_COI	GLTA-081	1	BE11468_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	AATTTAATTGTGACTAGGCATGGTCTG	151.18752
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11469_Cmae_Bot2011_COI	GLTA-082	1	BE11469_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	ATGAACAGTCTATCCTCCTTTAG	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11469_Hsan_Wil2021_COI	GLTA-082	1	BE11469_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	CCTGGGCCGGTATAGTAGGT	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11469_Mros_Gib2022_COI	GLTA-082	1	BE11469_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11469_Msal_Bot2012_COI	GLTA-082	1	BE11469_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	GYTAGTTCCRATGATGTTAGCTG	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11469_Mstr_Wil2021_COI	GLTA-082	1	BE11469_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	GTAGTGGTAACTACTCATGCTTTA	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11469_Pvir_Dia2013_COI	GLTA-082	1	BE11469_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	CTTAGTGGCATTAATTCGDAATCC	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11469_Rhar_Sim2018_COI	GLTA-082	1	BE11469_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	CTGCTGCTATTGCTCATG	151.18347
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11470_Aamu_Bax2006_COI	GLTA-083	1	BE11470_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	GCACAACCGGGATCTTTACTTC	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11470_Pcan_Bot2011_IGS	GLTA-083	1	BE11470_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-083	IGS	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	CGTAATCCTCAGTACTGGCTA	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11470_Pper_Dia2013_COI	GLTA-083	1	BE11470_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	CTTAGTGGCATTAATTCGDAATCC	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11470_Sspa_Oph2007_COI	GLTA-083	1	BE11470_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	GAAGGCCTTCCGATGATC	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11470_Upin_Bot2011_COI	GLTA-083	1	BE11470_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	CTTTAATTACAGCGTTTTTATTGTTGT	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11470_Vgib_Wil2025_COI	GLTA-083	1	BE11470_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	CCCCTCTTTACAAGTTCTTTAGGGT	151.18476
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11471_Asen_Wil2025_COI	GLTA-084	1	BE11471_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	GTTGTTGTTACAACACATGCTCTTTTG	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11471_Cjap_Sim2018_COI	GLTA-084	1	BE11471_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11471_Esin_And2018_Cyb	GLTA-084	1	BE11471_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-084	Cytb	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	ACCCCTCCTCATATCCAACCA	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11471_Mgig_Bot2012_COI	GLTA-084	1	BE11471_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	TCTTATTCGTTGGAGACTTTATAACCCT	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11471_Mjap_Gib2022_COI	GLTA-084	1	BE11471_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	AATTTAATTGTGACTAGGCATGGTCTG	151.18654
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11472_Cmae_Bot2011_COI	GLTA-085	1	BE11472_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	ATGAACAGTCTATCCTCCTTTAG	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11472_Hsan_Wil2021_COI	GLTA-085	1	BE11472_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	CCTGGGCCGGTATAGTAGGT	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11472_Mros_Gib2022_COI	GLTA-085	1	BE11472_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11472_Msal_Bot2012_COI	GLTA-085	1	BE11472_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	GYTAGTTCCRATGATGTTAGCTG	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11472_Mstr_Wil2021_COI	GLTA-085	1	BE11472_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	GTAGTGGTAACTACTCATGCTTTA	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11472_Pvir_Dia2013_COI	GLTA-085	1	BE11472_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	CTTAGTGGCATTAATTCGDAATCC	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11472_Rhar_Sim2018_COI	GLTA-085	1	BE11472_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	CTGCTGCTATTGCTCATG	151.18227
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11474_Aamu_Bax2006_COI	GLTA-087	1	BE11474_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	GCACAACCGGGATCTTTACTTC	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11474_Pcan_Bot2011_IGS	GLTA-087	1	BE11474_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-087	IGS	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	CGTAATCCTCAGTACTGGCTA	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11474_Pper_Dia2013_COI	GLTA-087	1	BE11474_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	CTTAGTGGCATTAATTCGDAATCC	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11474_Sspa_Oph2007_COI	GLTA-087	1	BE11474_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	GAAGGCCTTCCGATGATC	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11474_Upin_Bot2011_COI	GLTA-087	1	BE11474_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	CTTTAATTACAGCGTTTTTATTGTTGT	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11474_Vgib_Wil2025_COI	GLTA-087	1	BE11474_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	CCCCTCTTTACAAGTTCTTTAGGGT	151.18564
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11475_Asen_Wil2025_COI	GLTA-088	1	BE11475_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	GTTGTTGTTACAACACATGCTCTTTTG	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11475_Cjap_Sim2018_COI	GLTA-088	1	BE11475_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11475_Esin_And2018_Cyb	GLTA-088	1	BE11475_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-088	Cytb	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	ACCCCTCCTCATATCCAACCA	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11475_Mgig_Bot2012_COI	GLTA-088	1	BE11475_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	TCTTATTCGTTGGAGACTTTATAACCCT	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11475_Mjap_Gib2022_COI	GLTA-088	1	BE11475_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	AATTTAATTGTGACTAGGCATGGTCTG	151.18114
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11476_Cmae_Bot2011_COI	GLTA-089	1	BE11476_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	ATGAACAGTCTATCCTCCTTTAG	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11476_Hsan_Wil2021_COI	GLTA-089	1	BE11476_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	CCTGGGCCGGTATAGTAGGT	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11476_Mros_Gib2022_COI	GLTA-089	1	BE11476_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11476_Msal_Bot2012_COI	GLTA-089	1	BE11476_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	GYTAGTTCCRATGATGTTAGCTG	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11476_Mstr_Wil2021_COI	GLTA-089	1	BE11476_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	GTAGTGGTAACTACTCATGCTTTA	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11476_Pvir_Dia2013_COI	GLTA-089	1	BE11476_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	CTTAGTGGCATTAATTCGDAATCC	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11476_Rhar_Sim2018_COI	GLTA-089	1	BE11476_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	CTGCTGCTATTGCTCATG	151.18258
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11478_Aamu_Bax2006_COI	GLTA-091	1	BE11478_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	GCACAACCGGGATCTTTACTTC	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11478_Pcan_Bot2011_IGS	GLTA-091	1	BE11478_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-091	IGS	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	CGTAATCCTCAGTACTGGCTA	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11478_Pper_Dia2013_COI	GLTA-091	1	BE11478_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	CTTAGTGGCATTAATTCGDAATCC	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11478_Sspa_Oph2007_COI	GLTA-091	1	BE11478_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	GAAGGCCTTCCGATGATC	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11478_Upin_Bot2011_COI	GLTA-091	1	BE11478_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	CTTTAATTACAGCGTTTTTATTGTTGT	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11478_Vgib_Wil2025_COI	GLTA-091	1	BE11478_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	CCCCTCTTTACAAGTTCTTTAGGGT	151.1787
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11479_Asen_Wil2025_COI	GLTA-092	1	BE11479_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	GTTGTTGTTACAACACATGCTCTTTTG	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11479_Cjap_Sim2018_COI	GLTA-092	1	BE11479_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11479_Esin_And2018_Cyb	GLTA-092	1	BE11479_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-092	Cytb	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	ACCCCTCCTCATATCCAACCA	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11479_Mgig_Bot2012_COI	GLTA-092	1	BE11479_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	TCTTATTCGTTGGAGACTTTATAACCCT	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11479_Mjap_Gib2022_COI	GLTA-092	1	BE11479_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	AATTTAATTGTGACTAGGCATGGTCTG	151.1796
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11481_Aamu_Bax2006_COI	GLTA-094	1	BE11481_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	GCACAACCGGGATCTTTACTTC	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11481_Pcan_Bot2011_IGS	GLTA-094	1	BE11481_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-094	IGS	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	CGTAATCCTCAGTACTGGCTA	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11481_Pper_Dia2013_COI	GLTA-094	1	BE11481_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	CTTAGTGGCATTAATTCGDAATCC	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11481_Sspa_Oph2007_COI	GLTA-094	1	BE11481_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	GAAGGCCTTCCGATGATC	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11481_Upin_Bot2011_COI	GLTA-094	1	BE11481_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	CTTTAATTACAGCGTTTTTATTGTTGT	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11481_Vgib_Wil2025_COI	GLTA-094	1	BE11481_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	CCCCTCTTTACAAGTTCTTTAGGGT	151.1775
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11482_Asen_Wil2025_COI	GLTA-095	1	BE11482_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	GTTGTTGTTACAACACATGCTCTTTTG	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11482_Cjap_Sim2018_COI	GLTA-095	1	BE11482_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11482_Esin_And2018_Cyb	GLTA-095	1	BE11482_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-095	Cytb	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	ACCCCTCCTCATATCCAACCA	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11482_Mgig_Bot2012_COI	GLTA-095	1	BE11482_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	TCTTATTCGTTGGAGACTTTATAACCCT	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11482_Mjap_Gib2022_COI	GLTA-095	1	BE11482_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	AATTTAATTGTGACTAGGCATGGTCTG	151.17867
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11483_Cmae_Bot2011_COI	GLTA-096	1	BE11483_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	ATGAACAGTCTATCCTCCTTTAG	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11483_Hsan_Wil2021_COI	GLTA-096	1	BE11483_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	CCTGGGCCGGTATAGTAGGT	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11483_Mros_Gib2022_COI	GLTA-096	1	BE11483_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11483_Msal_Bot2012_COI	GLTA-096	1	BE11483_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	GYTAGTTCCRATGATGTTAGCTG	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11483_Mstr_Wil2021_COI	GLTA-096	1	BE11483_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	GTAGTGGTAACTACTCATGCTTTA	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11483_Pvir_Dia2013_COI	GLTA-096	1	BE11483_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	CTTAGTGGCATTAATTCGDAATCC	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11483_Rhar_Sim2018_COI	GLTA-096	1	BE11483_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	CTGCTGCTATTGCTCATG	151.18079
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11485_Aamu_Bax2006_COI	GLTA-098	1	BE11485_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	GCACAACCGGGATCTTTACTTC	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11485_Pcan_Bot2011_IGS	GLTA-098	1	BE11485_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-098	IGS	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	CGTAATCCTCAGTACTGGCTA	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11485_Pper_Dia2013_COI	GLTA-098	1	BE11485_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	CTTAGTGGCATTAATTCGDAATCC	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11485_Sspa_Oph2007_COI	GLTA-098	1	BE11485_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	GAAGGCCTTCCGATGATC	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11485_Upin_Bot2011_COI	GLTA-098	1	BE11485_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	CTTTAATTACAGCGTTTTTATTGTTGT	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11485_Vgib_Wil2025_COI	GLTA-098	1	BE11485_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	CCCCTCTTTACAAGTTCTTTAGGGT	151.17817
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11486_Asen_Wil2025_COI	GLTA-099	1	BE11486_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	GTTGTTGTTACAACACATGCTCTTTTG	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11486_Cjap_Sim2018_COI	GLTA-099	1	BE11486_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11486_Esin_And2018_Cyb	GLTA-099	1	BE11486_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-099	Cytb	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	ACCCCTCCTCATATCCAACCA	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11486_Mgig_Bot2012_COI	GLTA-099	1	BE11486_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	TCTTATTCGTTGGAGACTTTATAACCCT	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11486_Mjap_Gib2022_COI	GLTA-099	1	BE11486_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	AATTTAATTGTGACTAGGCATGGTCTG	151.1738
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11487_Cmae_Bot2011_COI	GLTA-100	1	BE11487_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	ATGAACAGTCTATCCTCCTTTAG	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11487_Hsan_Wil2021_COI	GLTA-100	1	BE11487_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	CCTGGGCCGGTATAGTAGGT	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11487_Mros_Gib2022_COI	GLTA-100	1	BE11487_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11487_Msal_Bot2012_COI	GLTA-100	1	BE11487_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	GYTAGTTCCRATGATGTTAGCTG	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11487_Mstr_Wil2021_COI	GLTA-100	1	BE11487_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	GTAGTGGTAACTACTCATGCTTTA	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11487_Pvir_Dia2013_COI	GLTA-100	1	BE11487_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	CTTAGTGGCATTAATTCGDAATCC	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11487_Rhar_Sim2018_COI	GLTA-100	1	BE11487_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	CTGCTGCTATTGCTCATG	151.17533
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11489_Aamu_Bax2006_COI	GLTA-102	1	BE11489_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	GCACAACCGGGATCTTTACTTC	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11489_Pcan_Bot2011_IGS	GLTA-102	1	BE11489_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-102	IGS	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	CGTAATCCTCAGTACTGGCTA	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11489_Pper_Dia2013_COI	GLTA-102	1	BE11489_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	CTTAGTGGCATTAATTCGDAATCC	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11489_Sspa_Oph2007_COI	GLTA-102	1	BE11489_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	GAAGGCCTTCCGATGATC	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11489_Upin_Bot2011_COI	GLTA-102	1	BE11489_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	CTTTAATTACAGCGTTTTTATTGTTGT	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11489_Vgib_Wil2025_COI	GLTA-102	1	BE11489_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	CCCCTCTTTACAAGTTCTTTAGGGT	151.17267
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11490_Cmae_Bot2011_COI	GLTA-103	1	BE11490_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	ATGAACAGTCTATCCTCCTTTAG	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11490_Hsan_Wil2021_COI	GLTA-103	1	BE11490_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	CCTGGGCCGGTATAGTAGGT	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11490_Mros_Gib2022_COI	GLTA-103	1	BE11490_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11490_Msal_Bot2012_COI	GLTA-103	1	BE11490_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	GYTAGTTCCRATGATGTTAGCTG	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11490_Mstr_Wil2021_COI	GLTA-103	1	BE11490_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	GTAGTGGTAACTACTCATGCTTTA	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11490_Pvir_Dia2013_COI	GLTA-103	1	BE11490_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	CTTAGTGGCATTAATTCGDAATCC	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11490_Rhar_Sim2018_COI	GLTA-103	1	BE11490_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	CTGCTGCTATTGCTCATG	151.17436
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11492_Aamu_Bax2006_COI	GLTA-105	1	BE11492_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	GCACAACCGGGATCTTTACTTC	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11492_Pcan_Bot2011_IGS	GLTA-105	1	BE11492_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-105	IGS	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	CGTAATCCTCAGTACTGGCTA	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11492_Pper_Dia2013_COI	GLTA-105	1	BE11492_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	CTTAGTGGCATTAATTCGDAATCC	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11492_Sspa_Oph2007_COI	GLTA-105	1	BE11492_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	GAAGGCCTTCCGATGATC	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11492_Upin_Bot2011_COI	GLTA-105	1	BE11492_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	CTTTAATTACAGCGTTTTTATTGTTGT	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11492_Vgib_Wil2025_COI	GLTA-105	1	BE11492_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	CCCCTCTTTACAAGTTCTTTAGGGT	151.17294
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11497_Asen_Wil2025_COI	GLTA-071	1	BE11497_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	GTTGTTGTTACAACACATGCTCTTTTG	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11497_Cjap_Sim2018_COI	GLTA-071	1	BE11497_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11497_Esin_And2018_Cyb	GLTA-071	1	BE11497_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-071	Cytb	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	ACCCCTCCTCATATCCAACCA	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11497_Mgig_Bot2012_COI	GLTA-071	1	BE11497_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	TCTTATTCGTTGGAGACTTTATAACCCT	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11497_Mjap_Gib2022_COI	GLTA-071	1	BE11497_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	AATTTAATTGTGACTAGGCATGGTCTG	151.1845
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11498_Cmae_Bot2011_COI	GLTA-072	1	BE11498_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	ATGAACAGTCTATCCTCCTTTAG	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11498_Hsan_Wil2021_COI	GLTA-072	1	BE11498_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	CCTGGGCCGGTATAGTAGGT	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11498_Mros_Gib2022_COI	GLTA-072	1	BE11498_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11498_Msal_Bot2012_COI	GLTA-072	1	BE11498_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	GYTAGTTCCRATGATGTTAGCTG	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11498_Mstr_Wil2021_COI	GLTA-072	1	BE11498_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	GTAGTGGTAACTACTCATGCTTTA	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11498_Pvir_Dia2013_COI	GLTA-072	1	BE11498_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	CTTAGTGGCATTAATTCGDAATCC	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11498_Rhar_Sim2018_COI	GLTA-072	1	BE11498_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	CTGCTGCTATTGCTCATG	151.18449
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11500_Aamu_Bax2006_COI	GLTA-074	1	BE11500_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	GCACAACCGGGATCTTTACTTC	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11500_Pcan_Bot2011_IGS	GLTA-074	1	BE11500_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-074	IGS	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	CGTAATCCTCAGTACTGGCTA	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11500_Pper_Dia2013_COI	GLTA-074	1	BE11500_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	CTTAGTGGCATTAATTCGDAATCC	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11500_Sspa_Oph2007_COI	GLTA-074	1	BE11500_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	GAAGGCCTTCCGATGATC	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11500_Upin_Bot2011_COI	GLTA-074	1	BE11500_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	CTTTAATTACAGCGTTTTTATTGTTGT	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11500_Vgib_Wil2025_COI	GLTA-074	1	BE11500_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	CCCCTCTTTACAAGTTCTTTAGGGT	151.18815
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11501_Asen_Wil2025_COI	GLTA-075	1	BE11501_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	GTTGTTGTTACAACACATGCTCTTTTG	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11501_Cjap_Sim2018_COI	GLTA-075	1	BE11501_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11501_Esin_And2018_Cyb	GLTA-075	1	BE11501_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-075	Cytb	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	ACCCCTCCTCATATCCAACCA	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11501_Mgig_Bot2012_COI	GLTA-075	1	BE11501_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	TCTTATTCGTTGGAGACTTTATAACCCT	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11501_Mjap_Gib2022_COI	GLTA-075	1	BE11501_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	AATTTAATTGTGACTAGGCATGGTCTG	151.1821
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11502_Cmae_Bot2011_COI	DRWA-001	1	BE11502_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	ATGAACAGTCTATCCTCCTTTAG	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11502_Hsan_Wil2021_COI	DRWA-001	1	BE11502_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	CCTGGGCCGGTATAGTAGGT	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11502_Mros_Gib2022_COI	DRWA-001	1	BE11502_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11502_Msal_Bot2012_COI	DRWA-001	1	BE11502_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	GYTAGTTCCRATGATGTTAGCTG	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11502_Mstr_Wil2021_COI	DRWA-001	1	BE11502_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	GTAGTGGTAACTACTCATGCTTTA	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11502_Pvir_Dia2013_COI	DRWA-001	1	BE11502_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	CTTAGTGGCATTAATTCGDAATCC	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11502_Rhar_Sim2018_COI	DRWA-001	1	BE11502_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	CTGCTGCTATTGCTCATG	130.84747
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11504_Aamu_Bax2006_COI	DRWA-003	1	BE11504_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	GCACAACCGGGATCTTTACTTC	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11504_Pcan_Bot2011_IGS	DRWA-003	1	BE11504_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-003	IGS	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	CGTAATCCTCAGTACTGGCTA	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11504_Pper_Dia2013_COI	DRWA-003	1	BE11504_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	CTTAGTGGCATTAATTCGDAATCC	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11504_Sspa_Oph2007_COI	DRWA-003	1	BE11504_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	GAAGGCCTTCCGATGATC	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11504_Upin_Bot2011_COI	DRWA-003	1	BE11504_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	CTTTAATTACAGCGTTTTTATTGTTGT	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11504_Vgib_Wil2025_COI	DRWA-003	1	BE11504_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	CCCCTCTTTACAAGTTCTTTAGGGT	130.85052
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11505_Asen_Wil2025_COI	DRWA-004	1	BE11505_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	GTTGTTGTTACAACACATGCTCTTTTG	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11505_Cjap_Sim2018_COI	DRWA-004	1	BE11505_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11505_Esin_And2018_Cyb	DRWA-004	1	BE11505_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-004	Cytb	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	ACCCCTCCTCATATCCAACCA	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11505_Mgig_Bot2012_COI	DRWA-004	1	BE11505_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	TCTTATTCGTTGGAGACTTTATAACCCT	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11505_Mjap_Gib2022_COI	DRWA-004	1	BE11505_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	AATTTAATTGTGACTAGGCATGGTCTG	130.852
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11506_Cmae_Bot2011_COI	DRWA-005	1	BE11506_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	ATGAACAGTCTATCCTCCTTTAG	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11506_Hsan_Wil2021_COI	DRWA-005	1	BE11506_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	CCTGGGCCGGTATAGTAGGT	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11506_Mros_Gib2022_COI	DRWA-005	1	BE11506_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11506_Msal_Bot2012_COI	DRWA-005	1	BE11506_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	GYTAGTTCCRATGATGTTAGCTG	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11506_Mstr_Wil2021_COI	DRWA-005	1	BE11506_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	GTAGTGGTAACTACTCATGCTTTA	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11506_Pvir_Dia2013_COI	DRWA-005	1	BE11506_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	CTTAGTGGCATTAATTCGDAATCC	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11506_Rhar_Sim2018_COI	DRWA-005	1	BE11506_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	CTGCTGCTATTGCTCATG	130.85324
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11508_Aamu_Bax2006_COI	DRWA-007	1	BE11508_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	GCACAACCGGGATCTTTACTTC	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11508_Pcan_Bot2011_IGS	DRWA-007	1	BE11508_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-007	IGS	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	CGTAATCCTCAGTACTGGCTA	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11508_Pper_Dia2013_COI	DRWA-007	1	BE11508_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	CTTAGTGGCATTAATTCGDAATCC	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11508_Sspa_Oph2007_COI	DRWA-007	1	BE11508_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	GAAGGCCTTCCGATGATC	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11508_Upin_Bot2011_COI	DRWA-007	1	BE11508_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	CTTTAATTACAGCGTTTTTATTGTTGT	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11508_Vgib_Wil2025_COI	DRWA-007	1	BE11508_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	CCCCTCTTTACAAGTTCTTTAGGGT	130.84857
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11509_Asen_Wil2025_COI	DRWA-008	1	BE11509_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	GTTGTTGTTACAACACATGCTCTTTTG	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11509_Cjap_Sim2018_COI	DRWA-008	1	BE11509_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11509_Esin_And2018_Cyb	DRWA-008	1	BE11509_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-008	Cytb	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	ACCCCTCCTCATATCCAACCA	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11509_Mgig_Bot2012_COI	DRWA-008	1	BE11509_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	TCTTATTCGTTGGAGACTTTATAACCCT	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11509_Mjap_Gib2022_COI	DRWA-008	1	BE11509_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	AATTTAATTGTGACTAGGCATGGTCTG	130.84983
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11511_Aamu_Bax2006_COI	DRWA-010	1	BE11511_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	GCACAACCGGGATCTTTACTTC	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11511_Pcan_Bot2011_IGS	DRWA-010	1	BE11511_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-010	IGS	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	CGTAATCCTCAGTACTGGCTA	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11511_Pper_Dia2013_COI	DRWA-010	1	BE11511_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	CTTAGTGGCATTAATTCGDAATCC	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11511_Sspa_Oph2007_COI	DRWA-010	1	BE11511_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	GAAGGCCTTCCGATGATC	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11511_Upin_Bot2011_COI	DRWA-010	1	BE11511_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	CTTTAATTACAGCGTTTTTATTGTTGT	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11511_Vgib_Wil2025_COI	DRWA-010	1	BE11511_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	CCCCTCTTTACAAGTTCTTTAGGGT	130.85253
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11512_Asen_Wil2025_COI	DRWA-011	1	BE11512_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	GTTGTTGTTACAACACATGCTCTTTTG	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11512_Cjap_Sim2018_COI	DRWA-011	1	BE11512_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11512_Esin_And2018_Cyb	DRWA-011	1	BE11512_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-011	Cytb	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	ACCCCTCCTCATATCCAACCA	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11512_Mgig_Bot2012_COI	DRWA-011	1	BE11512_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	TCTTATTCGTTGGAGACTTTATAACCCT	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11512_Mjap_Gib2022_COI	DRWA-011	1	BE11512_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	AATTTAATTGTGACTAGGCATGGTCTG	130.85392
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11513_Cmae_Bot2011_COI	DRWA-012	1	BE11513_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	ATGAACAGTCTATCCTCCTTTAG	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11513_Hsan_Wil2021_COI	DRWA-012	1	BE11513_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	CCTGGGCCGGTATAGTAGGT	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11513_Mros_Gib2022_COI	DRWA-012	1	BE11513_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11513_Msal_Bot2012_COI	DRWA-012	1	BE11513_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	GYTAGTTCCRATGATGTTAGCTG	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11513_Mstr_Wil2021_COI	DRWA-012	1	BE11513_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	GTAGTGGTAACTACTCATGCTTTA	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11513_Pvir_Dia2013_COI	DRWA-012	1	BE11513_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	CTTAGTGGCATTAATTCGDAATCC	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11513_Rhar_Sim2018_COI	DRWA-012	1	BE11513_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	CTGCTGCTATTGCTCATG	130.84598
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11515_Aamu_Bax2006_COI	DRWA-014	1	BE11515_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	GCACAACCGGGATCTTTACTTC	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11515_Pcan_Bot2011_IGS	DRWA-014	1	BE11515_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-014	IGS	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	CGTAATCCTCAGTACTGGCTA	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11515_Pper_Dia2013_COI	DRWA-014	1	BE11515_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	CTTAGTGGCATTAATTCGDAATCC	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11515_Sspa_Oph2007_COI	DRWA-014	1	BE11515_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	GAAGGCCTTCCGATGATC	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11515_Upin_Bot2011_COI	DRWA-014	1	BE11515_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	CTTTAATTACAGCGTTTTTATTGTTGT	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11515_Vgib_Wil2025_COI	DRWA-014	1	BE11515_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	CCCCTCTTTACAAGTTCTTTAGGGT	130.84539
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11516_Asen_Wil2025_COI	DRWA-015	1	BE11516_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	GTTGTTGTTACAACACATGCTCTTTTG	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11516_Cjap_Sim2018_COI	DRWA-015	1	BE11516_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11516_Esin_And2018_Cyb	DRWA-015	1	BE11516_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-015	Cytb	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	ACCCCTCCTCATATCCAACCA	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11516_Mgig_Bot2012_COI	DRWA-015	1	BE11516_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	TCTTATTCGTTGGAGACTTTATAACCCT	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11516_Mjap_Gib2022_COI	DRWA-015	1	BE11516_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	AATTTAATTGTGACTAGGCATGGTCTG	130.84672
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11517_Cmae_Bot2011_COI	DRWA-016	1	BE11517_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	ATGAACAGTCTATCCTCCTTTAG	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11517_Hsan_Wil2021_COI	DRWA-016	1	BE11517_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	CCTGGGCCGGTATAGTAGGT	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11517_Mros_Gib2022_COI	DRWA-016	1	BE11517_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11517_Msal_Bot2012_COI	DRWA-016	1	BE11517_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	GYTAGTTCCRATGATGTTAGCTG	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11517_Mstr_Wil2021_COI	DRWA-016	1	BE11517_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	GTAGTGGTAACTACTCATGCTTTA	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11517_Pvir_Dia2013_COI	DRWA-016	1	BE11517_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	CTTAGTGGCATTAATTCGDAATCC	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11517_Rhar_Sim2018_COI	DRWA-016	1	BE11517_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	CTGCTGCTATTGCTCATG	130.84482
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11519_Aamu_Bax2006_COI	DRWA-018	1	BE11519_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	GCACAACCGGGATCTTTACTTC	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11519_Pcan_Bot2011_IGS	DRWA-018	1	BE11519_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-018	IGS	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	CGTAATCCTCAGTACTGGCTA	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11519_Pper_Dia2013_COI	DRWA-018	1	BE11519_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	CTTAGTGGCATTAATTCGDAATCC	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11519_Sspa_Oph2007_COI	DRWA-018	1	BE11519_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	GAAGGCCTTCCGATGATC	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11519_Upin_Bot2011_COI	DRWA-018	1	BE11519_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	CTTTAATTACAGCGTTTTTATTGTTGT	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11519_Vgib_Wil2025_COI	DRWA-018	1	BE11519_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	CCCCTCTTTACAAGTTCTTTAGGGT	130.84534
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11520_Cmae_Bot2011_COI	DRWA-019	1	BE11520_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	ATGAACAGTCTATCCTCCTTTAG	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11520_Hsan_Wil2021_COI	DRWA-019	1	BE11520_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	CCTGGGCCGGTATAGTAGGT	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11520_Mros_Gib2022_COI	DRWA-019	1	BE11520_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11520_Msal_Bot2012_COI	DRWA-019	1	BE11520_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	GYTAGTTCCRATGATGTTAGCTG	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11520_Mstr_Wil2021_COI	DRWA-019	1	BE11520_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	GTAGTGGTAACTACTCATGCTTTA	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11520_Pvir_Dia2013_COI	DRWA-019	1	BE11520_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	CTTAGTGGCATTAATTCGDAATCC	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11520_Rhar_Sim2018_COI	DRWA-019	1	BE11520_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	CTGCTGCTATTGCTCATG	130.84673
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11522_Aamu_Bax2006_COI	DRWA-021	1	BE11522_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	GCACAACCGGGATCTTTACTTC	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11522_Pcan_Bot2011_IGS	DRWA-021	1	BE11522_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-021	IGS	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	CGTAATCCTCAGTACTGGCTA	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11522_Pper_Dia2013_COI	DRWA-021	1	BE11522_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	CTTAGTGGCATTAATTCGDAATCC	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11522_Sspa_Oph2007_COI	DRWA-021	1	BE11522_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	GAAGGCCTTCCGATGATC	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11522_Upin_Bot2011_COI	DRWA-021	1	BE11522_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	CTTTAATTACAGCGTTTTTATTGTTGT	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11522_Vgib_Wil2025_COI	DRWA-021	1	BE11522_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	CCCCTCTTTACAAGTTCTTTAGGGT	130.84612
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11523_Asen_Wil2025_COI	DRWA-022	1	BE11523_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	GTTGTTGTTACAACACATGCTCTTTTG	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11523_Cjap_Sim2018_COI	DRWA-022	1	BE11523_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11523_Esin_And2018_Cyb	DRWA-022	1	BE11523_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-022	Cytb	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	ACCCCTCCTCATATCCAACCA	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11523_Mgig_Bot2012_COI	DRWA-022	1	BE11523_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	TCTTATTCGTTGGAGACTTTATAACCCT	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11523_Mjap_Gib2022_COI	DRWA-022	1	BE11523_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	AATTTAATTGTGACTAGGCATGGTCTG	130.84736
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11524_Cmae_Bot2011_COI	DRWA-023	1	BE11524_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	ATGAACAGTCTATCCTCCTTTAG	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11524_Hsan_Wil2021_COI	DRWA-023	1	BE11524_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	CCTGGGCCGGTATAGTAGGT	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11524_Mros_Gib2022_COI	DRWA-023	1	BE11524_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11524_Msal_Bot2012_COI	DRWA-023	1	BE11524_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	GYTAGTTCCRATGATGTTAGCTG	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11524_Mstr_Wil2021_COI	DRWA-023	1	BE11524_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	GTAGTGGTAACTACTCATGCTTTA	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11524_Pvir_Dia2013_COI	DRWA-023	1	BE11524_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	CTTAGTGGCATTAATTCGDAATCC	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11524_Rhar_Sim2018_COI	DRWA-023	1	BE11524_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	CTGCTGCTATTGCTCATG	130.84894
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11526_Aamu_Bax2006_COI	DRWA-025	1	BE11526_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	GCACAACCGGGATCTTTACTTC	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11526_Pcan_Bot2011_IGS	DRWA-025	1	BE11526_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-025	IGS	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	CGTAATCCTCAGTACTGGCTA	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11526_Pper_Dia2013_COI	DRWA-025	1	BE11526_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	CTTAGTGGCATTAATTCGDAATCC	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11526_Sspa_Oph2007_COI	DRWA-025	1	BE11526_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	GAAGGCCTTCCGATGATC	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11526_Upin_Bot2011_COI	DRWA-025	1	BE11526_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	CTTTAATTACAGCGTTTTTATTGTTGT	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11526_Vgib_Wil2025_COI	DRWA-025	1	BE11526_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	CCCCTCTTTACAAGTTCTTTAGGGT	130.84542
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11527_Asen_Wil2025_COI	DRWA-026	1	BE11527_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	GTTGTTGTTACAACACATGCTCTTTTG	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11527_Cjap_Sim2018_COI	DRWA-026	1	BE11527_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11527_Esin_And2018_Cyb	DRWA-026	1	BE11527_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-026	Cytb	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	ACCCCTCCTCATATCCAACCA	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11527_Mgig_Bot2012_COI	DRWA-026	1	BE11527_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	TCTTATTCGTTGGAGACTTTATAACCCT	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11527_Mjap_Gib2022_COI	DRWA-026	1	BE11527_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	AATTTAATTGTGACTAGGCATGGTCTG	130.84675
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11528_Cmae_Bot2011_COI	DRWA-027	1	BE11528_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	ATGAACAGTCTATCCTCCTTTAG	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11528_Hsan_Wil2021_COI	DRWA-027	1	BE11528_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	CCTGGGCCGGTATAGTAGGT	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11528_Mros_Gib2022_COI	DRWA-027	1	BE11528_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11528_Msal_Bot2012_COI	DRWA-027	1	BE11528_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	GYTAGTTCCRATGATGTTAGCTG	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11528_Mstr_Wil2021_COI	DRWA-027	1	BE11528_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	GTAGTGGTAACTACTCATGCTTTA	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11528_Pvir_Dia2013_COI	DRWA-027	1	BE11528_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	CTTAGTGGCATTAATTCGDAATCC	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11528_Rhar_Sim2018_COI	DRWA-027	1	BE11528_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	CTGCTGCTATTGCTCATG	130.848
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11530_Asen_Wil2025_COI	DRWA-029	1	BE11530_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	GTTGTTGTTACAACACATGCTCTTTTG	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11530_Cjap_Sim2018_COI	DRWA-029	1	BE11530_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11530_Esin_And2018_Cyb	DRWA-029	1	BE11530_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-029	Cytb	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	ACCCCTCCTCATATCCAACCA	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11530_Mjap_Gib2022_COI	DRWA-029	1	BE11530_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	AATTTAATTGTGACTAGGCATGGTCTG	130.84747
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11531_Cmae_Bot2011_COI	DRWA-030	1	BE11531_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	ATGAACAGTCTATCCTCCTTTAG	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11531_Hsan_Wil2021_COI	DRWA-030	1	BE11531_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	CCTGGGCCGGTATAGTAGGT	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11531_Mros_Gib2022_COI	DRWA-030	1	BE11531_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11531_Msal_Bot2012_COI	DRWA-030	1	BE11531_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	GYTAGTTCCRATGATGTTAGCTG	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11531_Mstr_Wil2021_COI	DRWA-030	1	BE11531_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	GTAGTGGTAACTACTCATGCTTTA	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11531_Pvir_Dia2013_COI	DRWA-030	1	BE11531_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	CTTAGTGGCATTAATTCGDAATCC	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11531_Rhar_Sim2018_COI	DRWA-030	1	BE11531_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	CTGCTGCTATTGCTCATG	130.84867
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11533_Aamu_Bax2006_COI	DRWA-032	1	BE11533_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	GCACAACCGGGATCTTTACTTC	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11533_Pcan_Bot2011_IGS	DRWA-032	1	BE11533_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-032	IGS	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	CGTAATCCTCAGTACTGGCTA	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11533_Pper_Dia2013_COI	DRWA-032	1	BE11533_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	CTTAGTGGCATTAATTCGDAATCC	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11533_Sspa_Oph2007_COI	DRWA-032	1	BE11533_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	GAAGGCCTTCCGATGATC	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11533_Upin_Bot2011_COI	DRWA-032	1	BE11533_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	CTTTAATTACAGCGTTTTTATTGTTGT	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11533_Vgib_Wil2025_COI	DRWA-032	1	BE11533_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	CCCCTCTTTACAAGTTCTTTAGGGT	130.85127
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11534_Asen_Wil2025_COI	DRWA-033	1	BE11534_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	GTTGTTGTTACAACACATGCTCTTTTG	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11534_Cjap_Sim2018_COI	DRWA-033	1	BE11534_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11534_Esin_And2018_Cyb	DRWA-033	1	BE11534_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-033	Cytb	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	ACCCCTCCTCATATCCAACCA	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11534_Mgig_Bot2012_COI	DRWA-033	1	BE11534_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	TCTTATTCGTTGGAGACTTTATAACCCT	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11534_Mjap_Gib2022_COI	DRWA-033	1	BE11534_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	AATTTAATTGTGACTAGGCATGGTCTG	130.84738
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11535_Cmae_Bot2011_COI	DRWA-034	1	BE11535_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	ATGAACAGTCTATCCTCCTTTAG	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11535_Hsan_Wil2021_COI	DRWA-034	1	BE11535_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	CCTGGGCCGGTATAGTAGGT	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11535_Mros_Gib2022_COI	DRWA-034	1	BE11535_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11535_Msal_Bot2012_COI	DRWA-034	1	BE11535_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	GYTAGTTCCRATGATGTTAGCTG	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11535_Mstr_Wil2021_COI	DRWA-034	1	BE11535_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	GTAGTGGTAACTACTCATGCTTTA	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11535_Pvir_Dia2013_COI	DRWA-034	1	BE11535_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	CTTAGTGGCATTAATTCGDAATCC	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11535_Rhar_Sim2018_COI	DRWA-034	1	BE11535_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	CTGCTGCTATTGCTCATG	130.84775
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11541_Asen_Wil2025_COI	DRWA-106	1	BE11541_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	GTTGTTGTTACAACACATGCTCTTTTG	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11541_Cjap_Sim2018_COI	DRWA-106	1	BE11541_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11541_Esin_And2018_Cyb	DRWA-106	1	BE11541_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-106	Cytb	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	ACCCCTCCTCATATCCAACCA	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11541_Mgig_Bot2012_COI	DRWA-106	1	BE11541_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	TCTTATTCGTTGGAGACTTTATAACCCT	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11541_Mjap_Gib2022_COI	DRWA-106	1	BE11541_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	AATTTAATTGTGACTAGGCATGGTCTG	130.90486
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11542_Cmae_Bot2011_COI	DRWA-107	1	BE11542_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	ATGAACAGTCTATCCTCCTTTAG	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11542_Hsan_Wil2021_COI	DRWA-107	1	BE11542_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	CCTGGGCCGGTATAGTAGGT	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11542_Mros_Gib2022_COI	DRWA-107	1	BE11542_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11542_Msal_Bot2012_COI	DRWA-107	1	BE11542_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	GYTAGTTCCRATGATGTTAGCTG	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11542_Mstr_Wil2021_COI	DRWA-107	1	BE11542_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	GTAGTGGTAACTACTCATGCTTTA	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11542_Pvir_Dia2013_COI	DRWA-107	1	BE11542_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	CTTAGTGGCATTAATTCGDAATCC	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11542_Rhar_Sim2018_COI	DRWA-107	1	BE11542_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	CTGCTGCTATTGCTCATG	130.9044
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11544_Aamu_Bax2006_COI	DRWA-109	1	BE11544_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	GCACAACCGGGATCTTTACTTC	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11544_Pcan_Bot2011_IGS	DRWA-109	1	BE11544_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-109	IGS	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	CGTAATCCTCAGTACTGGCTA	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11544_Pper_Dia2013_COI	DRWA-109	1	BE11544_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	CTTAGTGGCATTAATTCGDAATCC	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11544_Sspa_Oph2007_COI	DRWA-109	1	BE11544_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	GAAGGCCTTCCGATGATC	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11544_Upin_Bot2011_COI	DRWA-109	1	BE11544_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	CTTTAATTACAGCGTTTTTATTGTTGT	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11544_Vgib_Wil2025_COI	DRWA-109	1	BE11544_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	CCCCTCTTTACAAGTTCTTTAGGGT	130.90443
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11545_Asen_Wil2025_COI	DRWA-110	1	BE11545_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	GTTGTTGTTACAACACATGCTCTTTTG	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11545_Cjap_Sim2018_COI	DRWA-110	1	BE11545_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11545_Esin_And2018_Cyb	DRWA-110	1	BE11545_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-110	Cytb	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	ACCCCTCCTCATATCCAACCA	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11545_Mgig_Bot2012_COI	DRWA-110	1	BE11545_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	TCTTATTCGTTGGAGACTTTATAACCCT	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11545_Mjap_Gib2022_COI	DRWA-110	1	BE11545_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	AATTTAATTGTGACTAGGCATGGTCTG	130.90553
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11546_Cmae_Bot2011_COI	DRWA-111	1	BE11546_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	ATGAACAGTCTATCCTCCTTTAG	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11546_Hsan_Wil2021_COI	DRWA-111	1	BE11546_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	CCTGGGCCGGTATAGTAGGT	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11546_Mros_Gib2022_COI	DRWA-111	1	BE11546_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11546_Msal_Bot2012_COI	DRWA-111	1	BE11546_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	GYTAGTTCCRATGATGTTAGCTG	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11546_Mstr_Wil2021_COI	DRWA-111	1	BE11546_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	GTAGTGGTAACTACTCATGCTTTA	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11546_Pvir_Dia2013_COI	DRWA-111	1	BE11546_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	CTTAGTGGCATTAATTCGDAATCC	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11546_Rhar_Sim2018_COI	DRWA-111	1	BE11546_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	CTGCTGCTATTGCTCATG	130.90358
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11548_Aamu_Bax2006_COI	DRWA-113	1	BE11548_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	GCACAACCGGGATCTTTACTTC	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11548_Pcan_Bot2011_IGS	DRWA-113	1	BE11548_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-113	IGS	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	CGTAATCCTCAGTACTGGCTA	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11548_Pper_Dia2013_COI	DRWA-113	1	BE11548_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	CTTAGTGGCATTAATTCGDAATCC	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11548_Sspa_Oph2007_COI	DRWA-113	1	BE11548_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	GAAGGCCTTCCGATGATC	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11548_Upin_Bot2011_COI	DRWA-113	1	BE11548_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	CTTTAATTACAGCGTTTTTATTGTTGT	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11548_Vgib_Wil2025_COI	DRWA-113	1	BE11548_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	CCCCTCTTTACAAGTTCTTTAGGGT	130.90438
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11549_Asen_Wil2025_COI	DRWA-114	1	BE11549_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	GTTGTTGTTACAACACATGCTCTTTTG	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11549_Cjap_Sim2018_COI	DRWA-114	1	BE11549_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11549_Esin_And2018_Cyb	DRWA-114	1	BE11549_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-114	Cytb	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	ACCCCTCCTCATATCCAACCA	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11549_Mgig_Bot2012_COI	DRWA-114	1	BE11549_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	TCTTATTCGTTGGAGACTTTATAACCCT	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11549_Mjap_Gib2022_COI	DRWA-114	1	BE11549_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	AATTTAATTGTGACTAGGCATGGTCTG	130.90575
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11551_Aamu_Bax2006_COI	DRWA-116	1	BE11551_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	GCACAACCGGGATCTTTACTTC	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11551_Pcan_Bot2011_IGS	DRWA-116	1	BE11551_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-116	IGS	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	CGTAATCCTCAGTACTGGCTA	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11551_Pper_Dia2013_COI	DRWA-116	1	BE11551_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	CTTAGTGGCATTAATTCGDAATCC	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11551_Sspa_Oph2007_COI	DRWA-116	1	BE11551_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	GAAGGCCTTCCGATGATC	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11551_Upin_Bot2011_COI	DRWA-116	1	BE11551_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	CTTTAATTACAGCGTTTTTATTGTTGT	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11551_Vgib_Wil2025_COI	DRWA-116	1	BE11551_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	CCCCTCTTTACAAGTTCTTTAGGGT	130.90822
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11552_Asen_Wil2025_COI	DRWA-117	1	BE11552_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	GTTGTTGTTACAACACATGCTCTTTTG	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11552_Cjap_Sim2018_COI	DRWA-117	1	BE11552_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11552_Esin_And2018_Cyb	DRWA-117	1	BE11552_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-117	Cytb	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	ACCCCTCCTCATATCCAACCA	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11552_Mgig_Bot2012_COI	DRWA-117	1	BE11552_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	TCTTATTCGTTGGAGACTTTATAACCCT	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11552_Mjap_Gib2022_COI	DRWA-117	1	BE11552_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	AATTTAATTGTGACTAGGCATGGTCTG	130.90413
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11553_Cmae_Bot2011_COI	DRWA-118	1	BE11553_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	ATGAACAGTCTATCCTCCTTTAG	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11553_Hsan_Wil2021_COI	DRWA-118	1	BE11553_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	CCTGGGCCGGTATAGTAGGT	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11553_Mros_Gib2022_COI	DRWA-118	1	BE11553_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11553_Msal_Bot2012_COI	DRWA-118	1	BE11553_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	GYTAGTTCCRATGATGTTAGCTG	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11553_Mstr_Wil2021_COI	DRWA-118	1	BE11553_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	GTAGTGGTAACTACTCATGCTTTA	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11553_Pvir_Dia2013_COI	DRWA-118	1	BE11553_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	CTTAGTGGCATTAATTCGDAATCC	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11553_Rhar_Sim2018_COI	DRWA-118	1	BE11553_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	CTGCTGCTATTGCTCATG	130.90494
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11555_Aamu_Bax2006_COI	DRWA-120	1	BE11555_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	GCACAACCGGGATCTTTACTTC	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11555_Pcan_Bot2011_IGS	DRWA-120	1	BE11555_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-120	IGS	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	CGTAATCCTCAGTACTGGCTA	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11555_Pper_Dia2013_COI	DRWA-120	1	BE11555_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	CTTAGTGGCATTAATTCGDAATCC	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11555_Sspa_Oph2007_COI	DRWA-120	1	BE11555_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	GAAGGCCTTCCGATGATC	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11555_Upin_Bot2011_COI	DRWA-120	1	BE11555_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	CTTTAATTACAGCGTTTTTATTGTTGT	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11555_Vgib_Wil2025_COI	DRWA-120	1	BE11555_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	CCCCTCTTTACAAGTTCTTTAGGGT	130.90767
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11556_Asen_Wil2025_COI	DRWA-121	1	BE11556_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	GTTGTTGTTACAACACATGCTCTTTTG	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11556_Cjap_Sim2018_COI	DRWA-121	1	BE11556_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11556_Esin_And2018_Cyb	DRWA-121	1	BE11556_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-121	Cytb	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	ACCCCTCCTCATATCCAACCA	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11556_Mgig_Bot2012_COI	DRWA-121	1	BE11556_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	TCTTATTCGTTGGAGACTTTATAACCCT	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11556_Mjap_Gib2022_COI	DRWA-121	1	BE11556_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	AATTTAATTGTGACTAGGCATGGTCTG	130.90885
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11557_Cmae_Bot2011_COI	DRWA-122	1	BE11557_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	ATGAACAGTCTATCCTCCTTTAG	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11557_Hsan_Wil2021_COI	DRWA-122	1	BE11557_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	CCTGGGCCGGTATAGTAGGT	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11557_Mros_Gib2022_COI	DRWA-122	1	BE11557_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11557_Msal_Bot2012_COI	DRWA-122	1	BE11557_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	GYTAGTTCCRATGATGTTAGCTG	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11557_Mstr_Wil2021_COI	DRWA-122	1	BE11557_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	GTAGTGGTAACTACTCATGCTTTA	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11557_Pvir_Dia2013_COI	DRWA-122	1	BE11557_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	CTTAGTGGCATTAATTCGDAATCC	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11557_Rhar_Sim2018_COI	DRWA-122	1	BE11557_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	CTGCTGCTATTGCTCATG	130.91004
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11559_Aamu_Bax2006_COI	DRWA-124	1	BE11559_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	GCACAACCGGGATCTTTACTTC	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11559_Pcan_Bot2011_IGS	DRWA-124	1	BE11559_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-124	IGS	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	CGTAATCCTCAGTACTGGCTA	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11559_Pper_Dia2013_COI	DRWA-124	1	BE11559_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	CTTAGTGGCATTAATTCGDAATCC	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11559_Sspa_Oph2007_COI	DRWA-124	1	BE11559_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	GAAGGCCTTCCGATGATC	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11559_Upin_Bot2011_COI	DRWA-124	1	BE11559_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	CTTTAATTACAGCGTTTTTATTGTTGT	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11559_Vgib_Wil2025_COI	DRWA-124	1	BE11559_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	CCCCTCTTTACAAGTTCTTTAGGGT	130.91247
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11560_Cmae_Bot2011_COI	DRWA-125	1	BE11560_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	ATGAACAGTCTATCCTCCTTTAG	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11560_Hsan_Wil2021_COI	DRWA-125	1	BE11560_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	CCTGGGCCGGTATAGTAGGT	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11560_Mros_Gib2022_COI	DRWA-125	1	BE11560_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11560_Msal_Bot2012_COI	DRWA-125	1	BE11560_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	GYTAGTTCCRATGATGTTAGCTG	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11560_Mstr_Wil2021_COI	DRWA-125	1	BE11560_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	GTAGTGGTAACTACTCATGCTTTA	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11560_Pvir_Dia2013_COI	DRWA-125	1	BE11560_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	CTTAGTGGCATTAATTCGDAATCC	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11560_Rhar_Sim2018_COI	DRWA-125	1	BE11560_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	CTGCTGCTATTGCTCATG	130.91337
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11562_Aamu_Bax2006_COI	DRWA-127	1	BE11562_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	GCACAACCGGGATCTTTACTTC	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11562_Pcan_Bot2011_IGS	DRWA-127	1	BE11562_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-127	IGS	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	CGTAATCCTCAGTACTGGCTA	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11562_Pper_Dia2013_COI	DRWA-127	1	BE11562_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	CTTAGTGGCATTAATTCGDAATCC	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11562_Sspa_Oph2007_COI	DRWA-127	1	BE11562_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	GAAGGCCTTCCGATGATC	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11562_Upin_Bot2011_COI	DRWA-127	1	BE11562_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	CTTTAATTACAGCGTTTTTATTGTTGT	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11562_Vgib_Wil2025_COI	DRWA-127	1	BE11562_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	CCCCTCTTTACAAGTTCTTTAGGGT	130.90671
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11563_Asen_Wil2025_COI	DRWA-128	1	BE11563_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	GTTGTTGTTACAACACATGCTCTTTTG	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11563_Cjap_Sim2018_COI	DRWA-128	1	BE11563_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11563_Esin_And2018_Cyb	DRWA-128	1	BE11563_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-128	Cytb	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	ACCCCTCCTCATATCCAACCA	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11563_Mgig_Bot2012_COI	DRWA-128	1	BE11563_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	TCTTATTCGTTGGAGACTTTATAACCCT	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11563_Mjap_Gib2022_COI	DRWA-128	1	BE11563_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	AATTTAATTGTGACTAGGCATGGTCTG	130.90833
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11564_Cmae_Bot2011_COI	DRWA-129	1	BE11564_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	ATGAACAGTCTATCCTCCTTTAG	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11564_Hsan_Wil2021_COI	DRWA-129	1	BE11564_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	CCTGGGCCGGTATAGTAGGT	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11564_Mros_Gib2022_COI	DRWA-129	1	BE11564_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11564_Msal_Bot2012_COI	DRWA-129	1	BE11564_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	GYTAGTTCCRATGATGTTAGCTG	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11564_Mstr_Wil2021_COI	DRWA-129	1	BE11564_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	GTAGTGGTAACTACTCATGCTTTA	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11564_Pvir_Dia2013_COI	DRWA-129	1	BE11564_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	CTTAGTGGCATTAATTCGDAATCC	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11564_Rhar_Sim2018_COI	DRWA-129	1	BE11564_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	CTGCTGCTATTGCTCATG	130.90933
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11566_Aamu_Bax2006_COI	DRWA-131	1	BE11566_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	GCACAACCGGGATCTTTACTTC	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11566_Pcan_Bot2011_IGS	DRWA-131	1	BE11566_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-131	IGS	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	CGTAATCCTCAGTACTGGCTA	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11566_Pper_Dia2013_COI	DRWA-131	1	BE11566_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	CTTAGTGGCATTAATTCGDAATCC	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11566_Sspa_Oph2007_COI	DRWA-131	1	BE11566_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	GAAGGCCTTCCGATGATC	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11566_Upin_Bot2011_COI	DRWA-131	1	BE11566_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	CTTTAATTACAGCGTTTTTATTGTTGT	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11566_Vgib_Wil2025_COI	DRWA-131	1	BE11566_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	CCCCTCTTTACAAGTTCTTTAGGGT	130.91196
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11567_Asen_Wil2025_COI	DRWA-132	1	BE11567_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	GTTGTTGTTACAACACATGCTCTTTTG	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11567_Cjap_Sim2018_COI	DRWA-132	1	BE11567_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11567_Esin_And2018_Cyb	DRWA-132	1	BE11567_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-132	Cytb	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	ACCCCTCCTCATATCCAACCA	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11567_Mgig_Bot2012_COI	DRWA-132	1	BE11567_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	TCTTATTCGTTGGAGACTTTATAACCCT	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11567_Mjap_Gib2022_COI	DRWA-132	1	BE11567_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	AATTTAATTGTGACTAGGCATGGTCTG	130.91302
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11568_Cmae_Bot2011_COI	DRWA-133	1	BE11568_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	ATGAACAGTCTATCCTCCTTTAG	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11568_Hsan_Wil2021_COI	DRWA-133	1	BE11568_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	CCTGGGCCGGTATAGTAGGT	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11568_Mros_Gib2022_COI	DRWA-133	1	BE11568_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11568_Msal_Bot2012_COI	DRWA-133	1	BE11568_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	GYTAGTTCCRATGATGTTAGCTG	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11568_Mstr_Wil2021_COI	DRWA-133	1	BE11568_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	GTAGTGGTAACTACTCATGCTTTA	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11568_Pvir_Dia2013_COI	DRWA-133	1	BE11568_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	CTTAGTGGCATTAATTCGDAATCC	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11568_Rhar_Sim2018_COI	DRWA-133	1	BE11568_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	CTGCTGCTATTGCTCATG	130.91413
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11570_Asen_Wil2025_COI	DRWA-135	1	BE11570_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	GTTGTTGTTACAACACATGCTCTTTTG	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11570_Cjap_Sim2018_COI	DRWA-135	1	BE11570_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11570_Esin_And2018_Cyb	DRWA-135	1	BE11570_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-135	Cytb	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	ACCCCTCCTCATATCCAACCA	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11570_Mgig_Bot2012_COI	DRWA-135	1	BE11570_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	TCTTATTCGTTGGAGACTTTATAACCCT	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11570_Mjap_Gib2022_COI	DRWA-135	1	BE11570_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	AATTTAATTGTGACTAGGCATGGTCTG	130.90891
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11571_Cmae_Bot2011_COI	DRWA-136	1	BE11571_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	ATGAACAGTCTATCCTCCTTTAG	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11571_Hsan_Wil2021_COI	DRWA-136	1	BE11571_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	CCTGGGCCGGTATAGTAGGT	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11571_Mros_Gib2022_COI	DRWA-136	1	BE11571_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11571_Msal_Bot2012_COI	DRWA-136	1	BE11571_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	GYTAGTTCCRATGATGTTAGCTG	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11571_Mstr_Wil2021_COI	DRWA-136	1	BE11571_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	GTAGTGGTAACTACTCATGCTTTA	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11571_Pvir_Dia2013_COI	DRWA-136	1	BE11571_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	CTTAGTGGCATTAATTCGDAATCC	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11571_Rhar_Sim2018_COI	DRWA-136	1	BE11571_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	CTGCTGCTATTGCTCATG	130.91023
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11573_Aamu_Bax2006_COI	DRWA-138	1	BE11573_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	GCACAACCGGGATCTTTACTTC	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11573_Pcan_Bot2011_IGS	DRWA-138	1	BE11573_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-138	IGS	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	CGTAATCCTCAGTACTGGCTA	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11573_Pper_Dia2013_COI	DRWA-138	1	BE11573_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	CTTAGTGGCATTAATTCGDAATCC	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11573_Sspa_Oph2007_COI	DRWA-138	1	BE11573_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	GAAGGCCTTCCGATGATC	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11573_Upin_Bot2011_COI	DRWA-138	1	BE11573_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	CTTTAATTACAGCGTTTTTATTGTTGT	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11573_Vgib_Wil2025_COI	DRWA-138	1	BE11573_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	CCCCTCTTTACAAGTTCTTTAGGGT	130.91245
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11574_Asen_Wil2025_COI	DRWA-139	1	BE11574_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	GTTGTTGTTACAACACATGCTCTTTTG	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11574_Cjap_Sim2018_COI	DRWA-139	1	BE11574_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11574_Esin_And2018_Cyb	DRWA-139	1	BE11574_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-139	Cytb	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	ACCCCTCCTCATATCCAACCA	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11574_Mgig_Bot2012_COI	DRWA-139	1	BE11574_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	TCTTATTCGTTGGAGACTTTATAACCCT	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11574_Mjap_Gib2022_COI	DRWA-139	1	BE11574_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	AATTTAATTGTGACTAGGCATGGTCTG	130.91373
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11575_Cmae_Bot2011_COI	DRWA-140	1	BE11575_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	ATGAACAGTCTATCCTCCTTTAG	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11575_Hsan_Wil2021_COI	DRWA-140	1	BE11575_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	CCTGGGCCGGTATAGTAGGT	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11575_Mros_Gib2022_COI	DRWA-140	1	BE11575_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11575_Msal_Bot2012_COI	DRWA-140	1	BE11575_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	GYTAGTTCCRATGATGTTAGCTG	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11575_Mstr_Wil2021_COI	DRWA-140	1	BE11575_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	GTAGTGGTAACTACTCATGCTTTA	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11575_Pvir_Dia2013_COI	DRWA-140	1	BE11575_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	CTTAGTGGCATTAATTCGDAATCC	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11575_Rhar_Sim2018_COI	DRWA-140	1	BE11575_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	CTGCTGCTATTGCTCATG	130.91305
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11580_Aamu_Bax2006_COI	DRWA-036	1	BE11580_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	GCACAACCGGGATCTTTACTTC	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11580_Pcan_Bot2011_IGS	DRWA-036	1	BE11580_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-036	IGS	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	CGTAATCCTCAGTACTGGCTA	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11580_Pper_Dia2013_COI	DRWA-036	1	BE11580_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	CTTAGTGGCATTAATTCGDAATCC	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11580_Sspa_Oph2007_COI	DRWA-036	1	BE11580_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	GAAGGCCTTCCGATGATC	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11580_Upin_Bot2011_COI	DRWA-036	1	BE11580_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	CTTTAATTACAGCGTTTTTATTGTTGT	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11580_Vgib_Wil2025_COI	DRWA-036	1	BE11580_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	CCCCTCTTTACAAGTTCTTTAGGGT	130.93098
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11581_Asen_Wil2025_COI	DRWA-037	1	BE11581_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	GTTGTTGTTACAACACATGCTCTTTTG	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11581_Cjap_Sim2018_COI	DRWA-037	1	BE11581_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11581_Esin_And2018_Cyb	DRWA-037	1	BE11581_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-037	Cytb	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	ACCCCTCCTCATATCCAACCA	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11581_Mgig_Bot2012_COI	DRWA-037	1	BE11581_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	TCTTATTCGTTGGAGACTTTATAACCCT	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11581_Mjap_Gib2022_COI	DRWA-037	1	BE11581_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	AATTTAATTGTGACTAGGCATGGTCTG	130.92944
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11582_Cmae_Bot2011_COI	DRWA-038	1	BE11582_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	ATGAACAGTCTATCCTCCTTTAG	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11582_Hsan_Wil2021_COI	DRWA-038	1	BE11582_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	CCTGGGCCGGTATAGTAGGT	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11582_Mros_Gib2022_COI	DRWA-038	1	BE11582_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11582_Msal_Bot2012_COI	DRWA-038	1	BE11582_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	GYTAGTTCCRATGATGTTAGCTG	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11582_Mstr_Wil2021_COI	DRWA-038	1	BE11582_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	GTAGTGGTAACTACTCATGCTTTA	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11582_Pvir_Dia2013_COI	DRWA-038	1	BE11582_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	CTTAGTGGCATTAATTCGDAATCC	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11582_Rhar_Sim2018_COI	DRWA-038	1	BE11582_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	CTGCTGCTATTGCTCATG	130.93065
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11584_Aamu_Bax2006_COI	DRWA-040	1	BE11584_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	GCACAACCGGGATCTTTACTTC	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11584_Pcan_Bot2011_IGS	DRWA-040	1	BE11584_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-040	IGS	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	CGTAATCCTCAGTACTGGCTA	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11584_Pper_Dia2013_COI	DRWA-040	1	BE11584_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	CTTAGTGGCATTAATTCGDAATCC	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11584_Sspa_Oph2007_COI	DRWA-040	1	BE11584_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	GAAGGCCTTCCGATGATC	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11584_Upin_Bot2011_COI	DRWA-040	1	BE11584_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	CTTTAATTACAGCGTTTTTATTGTTGT	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11584_Vgib_Wil2025_COI	DRWA-040	1	BE11584_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	CCCCTCTTTACAAGTTCTTTAGGGT	130.92998
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11585_Asen_Wil2025_COI	DRWA-041	1	BE11585_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	GTTGTTGTTACAACACATGCTCTTTTG	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11585_Cjap_Sim2018_COI	DRWA-041	1	BE11585_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11585_Esin_And2018_Cyb	DRWA-041	1	BE11585_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-041	Cytb	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	ACCCCTCCTCATATCCAACCA	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11585_Mgig_Bot2012_COI	DRWA-041	1	BE11585_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	TCTTATTCGTTGGAGACTTTATAACCCT	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11585_Mjap_Gib2022_COI	DRWA-041	1	BE11585_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	AATTTAATTGTGACTAGGCATGGTCTG	130.93095
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11586_Cmae_Bot2011_COI	DRWA-042	1	BE11586_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	ATGAACAGTCTATCCTCCTTTAG	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11586_Hsan_Wil2021_COI	DRWA-042	1	BE11586_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	CCTGGGCCGGTATAGTAGGT	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11586_Mros_Gib2022_COI	DRWA-042	1	BE11586_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11586_Msal_Bot2012_COI	DRWA-042	1	BE11586_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	GYTAGTTCCRATGATGTTAGCTG	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11586_Mstr_Wil2021_COI	DRWA-042	1	BE11586_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	GTAGTGGTAACTACTCATGCTTTA	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11586_Pvir_Dia2013_COI	DRWA-042	1	BE11586_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	CTTAGTGGCATTAATTCGDAATCC	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11586_Rhar_Sim2018_COI	DRWA-042	1	BE11586_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	CTGCTGCTATTGCTCATG	130.93261
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11588_Aamu_Bax2006_COI	DRWA-044	1	BE11588_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	GCACAACCGGGATCTTTACTTC	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11588_Pcan_Bot2011_IGS	DRWA-044	1	BE11588_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-044	IGS	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	CGTAATCCTCAGTACTGGCTA	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11588_Pper_Dia2013_COI	DRWA-044	1	BE11588_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	CTTAGTGGCATTAATTCGDAATCC	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11588_Sspa_Oph2007_COI	DRWA-044	1	BE11588_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	GAAGGCCTTCCGATGATC	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11588_Upin_Bot2011_COI	DRWA-044	1	BE11588_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	CTTTAATTACAGCGTTTTTATTGTTGT	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11588_Vgib_Wil2025_COI	DRWA-044	1	BE11588_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	CCCCTCTTTACAAGTTCTTTAGGGT	130.93111
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11589_Asen_Wil2025_COI	DRWA-045	1	BE11589_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	GTTGTTGTTACAACACATGCTCTTTTG	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11589_Cjap_Sim2018_COI	DRWA-045	1	BE11589_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11589_Esin_And2018_Cyb	DRWA-045	1	BE11589_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-045	Cytb	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	ACCCCTCCTCATATCCAACCA	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11589_Mgig_Bot2012_COI	DRWA-045	1	BE11589_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	TCTTATTCGTTGGAGACTTTATAACCCT	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11589_Mjap_Gib2022_COI	DRWA-045	1	BE11589_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	AATTTAATTGTGACTAGGCATGGTCTG	130.9322
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11591_Aamu_Bax2006_COI	DRWA-047	1	BE11591_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	GCACAACCGGGATCTTTACTTC	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11591_Pcan_Bot2011_IGS	DRWA-047	1	BE11591_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-047	IGS	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	CGTAATCCTCAGTACTGGCTA	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11591_Pper_Dia2013_COI	DRWA-047	1	BE11591_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	CTTAGTGGCATTAATTCGDAATCC	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11591_Sspa_Oph2007_COI	DRWA-047	1	BE11591_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	GAAGGCCTTCCGATGATC	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11591_Upin_Bot2011_COI	DRWA-047	1	BE11591_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	CTTTAATTACAGCGTTTTTATTGTTGT	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11591_Vgib_Wil2025_COI	DRWA-047	1	BE11591_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	CCCCTCTTTACAAGTTCTTTAGGGT	130.93074
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11592_Asen_Wil2025_COI	DRWA-048	1	BE11592_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	GTTGTTGTTACAACACATGCTCTTTTG	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11592_Cjap_Sim2018_COI	DRWA-048	1	BE11592_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11592_Esin_And2018_Cyb	DRWA-048	1	BE11592_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-048	Cytb	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	ACCCCTCCTCATATCCAACCA	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11592_Mgig_Bot2012_COI	DRWA-048	1	BE11592_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	TCTTATTCGTTGGAGACTTTATAACCCT	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11592_Mjap_Gib2022_COI	DRWA-048	1	BE11592_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	AATTTAATTGTGACTAGGCATGGTCTG	130.9317
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11593_Cmae_Bot2011_COI	DRWA-049	1	BE11593_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	ATGAACAGTCTATCCTCCTTTAG	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11593_Hsan_Wil2021_COI	DRWA-049	1	BE11593_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	CCTGGGCCGGTATAGTAGGT	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11593_Mros_Gib2022_COI	DRWA-049	1	BE11593_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11593_Msal_Bot2012_COI	DRWA-049	1	BE11593_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	GYTAGTTCCRATGATGTTAGCTG	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11593_Mstr_Wil2021_COI	DRWA-049	1	BE11593_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	GTAGTGGTAACTACTCATGCTTTA	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11593_Pvir_Dia2013_COI	DRWA-049	1	BE11593_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	CTTAGTGGCATTAATTCGDAATCC	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11593_Rhar_Sim2018_COI	DRWA-049	1	BE11593_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	CTGCTGCTATTGCTCATG	130.92916
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11595_Aamu_Bax2006_COI	DRWA-051	1	BE11595_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	GCACAACCGGGATCTTTACTTC	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11595_Pcan_Bot2011_IGS	DRWA-051	1	BE11595_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-051	IGS	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	CGTAATCCTCAGTACTGGCTA	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11595_Pper_Dia2013_COI	DRWA-051	1	BE11595_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	CTTAGTGGCATTAATTCGDAATCC	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11595_Sspa_Oph2007_COI	DRWA-051	1	BE11595_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	GAAGGCCTTCCGATGATC	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11595_Upin_Bot2011_COI	DRWA-051	1	BE11595_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	CTTTAATTACAGCGTTTTTATTGTTGT	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11595_Vgib_Wil2025_COI	DRWA-051	1	BE11595_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	CCCCTCTTTACAAGTTCTTTAGGGT	130.93119
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11596_Asen_Wil2025_COI	DRWA-052	1	BE11596_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	GTTGTTGTTACAACACATGCTCTTTTG	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11596_Cjap_Sim2018_COI	DRWA-052	1	BE11596_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11596_Esin_And2018_Cyb	DRWA-052	1	BE11596_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-052	Cytb	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	ACCCCTCCTCATATCCAACCA	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11596_Mgig_Bot2012_COI	DRWA-052	1	BE11596_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	TCTTATTCGTTGGAGACTTTATAACCCT	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11596_Mjap_Gib2022_COI	DRWA-052	1	BE11596_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	AATTTAATTGTGACTAGGCATGGTCTG	130.9322
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11597_Cmae_Bot2011_COI	DRWA-053	1	BE11597_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	ATGAACAGTCTATCCTCCTTTAG	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11597_Hsan_Wil2021_COI	DRWA-053	1	BE11597_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	CCTGGGCCGGTATAGTAGGT	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11597_Mros_Gib2022_COI	DRWA-053	1	BE11597_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11597_Msal_Bot2012_COI	DRWA-053	1	BE11597_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	GYTAGTTCCRATGATGTTAGCTG	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11597_Mstr_Wil2021_COI	DRWA-053	1	BE11597_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	GTAGTGGTAACTACTCATGCTTTA	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11597_Pvir_Dia2013_COI	DRWA-053	1	BE11597_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	CTTAGTGGCATTAATTCGDAATCC	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11597_Rhar_Sim2018_COI	DRWA-053	1	BE11597_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	CTGCTGCTATTGCTCATG	130.9286
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11599_Aamu_Bax2006_COI	DRWA-055	1	BE11599_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	GCACAACCGGGATCTTTACTTC	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11599_Pcan_Bot2011_IGS	DRWA-055	1	BE11599_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-055	IGS	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	CGTAATCCTCAGTACTGGCTA	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11599_Pper_Dia2013_COI	DRWA-055	1	BE11599_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	CTTAGTGGCATTAATTCGDAATCC	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11599_Sspa_Oph2007_COI	DRWA-055	1	BE11599_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	GAAGGCCTTCCGATGATC	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11599_Upin_Bot2011_COI	DRWA-055	1	BE11599_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	CTTTAATTACAGCGTTTTTATTGTTGT	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11599_Vgib_Wil2025_COI	DRWA-055	1	BE11599_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	CCCCTCTTTACAAGTTCTTTAGGGT	130.93069
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11600_Asen_Wil2025_COI	DRWA-056	1	BE11600_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	GTTGTTGTTACAACACATGCTCTTTTG	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11600_Cjap_Sim2018_COI	DRWA-056	1	BE11600_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11600_Esin_And2018_Cyb	DRWA-056	1	BE11600_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-056	Cytb	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	ACCCCTCCTCATATCCAACCA	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11600_Mgig_Bot2012_COI	DRWA-056	1	BE11600_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	TCTTATTCGTTGGAGACTTTATAACCCT	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11600_Mjap_Gib2022_COI	DRWA-056	1	BE11600_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	AATTTAATTGTGACTAGGCATGGTCTG	130.93181
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11601_Cmae_Bot2011_COI	DRWA-057	1	BE11601_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	ATGAACAGTCTATCCTCCTTTAG	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11601_Hsan_Wil2021_COI	DRWA-057	1	BE11601_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	CCTGGGCCGGTATAGTAGGT	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11601_Mros_Gib2022_COI	DRWA-057	1	BE11601_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11601_Msal_Bot2012_COI	DRWA-057	1	BE11601_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	GYTAGTTCCRATGATGTTAGCTG	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11601_Mstr_Wil2021_COI	DRWA-057	1	BE11601_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	GTAGTGGTAACTACTCATGCTTTA	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11601_Pvir_Dia2013_COI	DRWA-057	1	BE11601_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	CTTAGTGGCATTAATTCGDAATCC	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11601_Rhar_Sim2018_COI	DRWA-057	1	BE11601_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	CTGCTGCTATTGCTCATG	130.92821
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11603_Aamu_Bax2006_COI	DRWA-059	1	BE11603_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	GCACAACCGGGATCTTTACTTC	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11603_Pcan_Bot2011_IGS	DRWA-059	1	BE11603_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-059	IGS	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	CGTAATCCTCAGTACTGGCTA	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11603_Pper_Dia2013_COI	DRWA-059	1	BE11603_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	CTTAGTGGCATTAATTCGDAATCC	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11603_Sspa_Oph2007_COI	DRWA-059	1	BE11603_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	GAAGGCCTTCCGATGATC	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11603_Upin_Bot2011_COI	DRWA-059	1	BE11603_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	CTTTAATTACAGCGTTTTTATTGTTGT	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11603_Vgib_Wil2025_COI	DRWA-059	1	BE11603_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	CCCCTCTTTACAAGTTCTTTAGGGT	130.93037
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11604_Asen_Wil2025_COI	DRWA-060	1	BE11604_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	GTTGTTGTTACAACACATGCTCTTTTG	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11604_Cjap_Sim2018_COI	DRWA-060	1	BE11604_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11604_Esin_And2018_Cyb	DRWA-060	1	BE11604_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-060	Cytb	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	ACCCCTCCTCATATCCAACCA	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11604_Mgig_Bot2012_COI	DRWA-060	1	BE11604_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	TCTTATTCGTTGGAGACTTTATAACCCT	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11604_Mjap_Gib2022_COI	DRWA-060	1	BE11604_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	AATTTAATTGTGACTAGGCATGGTCTG	130.93128
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11605_Cmae_Bot2011_COI	DRWA-061	1	BE11605_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	ATGAACAGTCTATCCTCCTTTAG	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11605_Hsan_Wil2021_COI	DRWA-061	1	BE11605_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	CCTGGGCCGGTATAGTAGGT	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11605_Mros_Gib2022_COI	DRWA-061	1	BE11605_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11605_Msal_Bot2012_COI	DRWA-061	1	BE11605_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	GYTAGTTCCRATGATGTTAGCTG	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11605_Mstr_Wil2021_COI	DRWA-061	1	BE11605_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	GTAGTGGTAACTACTCATGCTTTA	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11605_Pvir_Dia2013_COI	DRWA-061	1	BE11605_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	CTTAGTGGCATTAATTCGDAATCC	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11605_Rhar_Sim2018_COI	DRWA-061	1	BE11605_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	CTGCTGCTATTGCTCATG	130.92762
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11607_Aamu_Bax2006_COI	DRWA-063	1	BE11607_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	GCACAACCGGGATCTTTACTTC	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11607_Pcan_Bot2011_IGS	DRWA-063	1	BE11607_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-063	IGS	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	CGTAATCCTCAGTACTGGCTA	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11607_Pper_Dia2013_COI	DRWA-063	1	BE11607_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	CTTAGTGGCATTAATTCGDAATCC	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11607_Sspa_Oph2007_COI	DRWA-063	1	BE11607_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	GAAGGCCTTCCGATGATC	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11607_Upin_Bot2011_COI	DRWA-063	1	BE11607_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	CTTTAATTACAGCGTTTTTATTGTTGT	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11607_Vgib_Wil2025_COI	DRWA-063	1	BE11607_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	CCCCTCTTTACAAGTTCTTTAGGGT	130.92967
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11608_Asen_Wil2025_COI	DRWA-064	1	BE11608_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	GTTGTTGTTACAACACATGCTCTTTTG	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11608_Cjap_Sim2018_COI	DRWA-064	1	BE11608_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11608_Esin_And2018_Cyb	DRWA-064	1	BE11608_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-064	Cytb	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	ACCCCTCCTCATATCCAACCA	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11608_Mgig_Bot2012_COI	DRWA-064	1	BE11608_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	TCTTATTCGTTGGAGACTTTATAACCCT	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11608_Mjap_Gib2022_COI	DRWA-064	1	BE11608_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	AATTTAATTGTGACTAGGCATGGTCTG	130.93076
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11609_Cmae_Bot2011_COI	DRWA-065	1	BE11609_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	ATGAACAGTCTATCCTCCTTTAG	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11609_Hsan_Wil2021_COI	DRWA-065	1	BE11609_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	CCTGGGCCGGTATAGTAGGT	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11609_Mros_Gib2022_COI	DRWA-065	1	BE11609_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11609_Msal_Bot2012_COI	DRWA-065	1	BE11609_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	GYTAGTTCCRATGATGTTAGCTG	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11609_Mstr_Wil2021_COI	DRWA-065	1	BE11609_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	GTAGTGGTAACTACTCATGCTTTA	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11609_Pvir_Dia2013_COI	DRWA-065	1	BE11609_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	CTTAGTGGCATTAATTCGDAATCC	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11609_Rhar_Sim2018_COI	DRWA-065	1	BE11609_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	CTGCTGCTATTGCTCATG	130.92723
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11614_Aamu_Bax2006_COI	DRWA-066	1	BE11614_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	GCACAACCGGGATCTTTACTTC	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11614_Pcan_Bot2011_IGS	DRWA-066	1	BE11614_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-066	IGS	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	CGTAATCCTCAGTACTGGCTA	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11614_Pper_Dia2013_COI	DRWA-066	1	BE11614_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	CTTAGTGGCATTAATTCGDAATCC	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11614_Sspa_Oph2007_COI	DRWA-066	1	BE11614_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	GAAGGCCTTCCGATGATC	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11614_Upin_Bot2011_COI	DRWA-066	1	BE11614_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	CTTTAATTACAGCGTTTTTATTGTTGT	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11614_Vgib_Wil2025_COI	DRWA-066	1	BE11614_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	CCCCTCTTTACAAGTTCTTTAGGGT	130.92805
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11615_Asen_Wil2025_COI	DRWA-067	1	BE11615_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	GTTGTTGTTACAACACATGCTCTTTTG	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11615_Cjap_Sim2018_COI	DRWA-067	1	BE11615_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11615_Esin_And2018_Cyb	DRWA-067	1	BE11615_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-067	Cytb	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	ACCCCTCCTCATATCCAACCA	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11615_Mgig_Bot2012_COI	DRWA-067	1	BE11615_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	TCTTATTCGTTGGAGACTTTATAACCCT	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11615_Mjap_Gib2022_COI	DRWA-067	1	BE11615_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	AATTTAATTGTGACTAGGCATGGTCTG	130.92913
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11616_Cmae_Bot2011_COI	DRWA-068	1	BE11616_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	ATGAACAGTCTATCCTCCTTTAG	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11616_Hsan_Wil2021_COI	DRWA-068	1	BE11616_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	CCTGGGCCGGTATAGTAGGT	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11616_Mros_Gib2022_COI	DRWA-068	1	BE11616_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11616_Msal_Bot2012_COI	DRWA-068	1	BE11616_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	GYTAGTTCCRATGATGTTAGCTG	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11616_Mstr_Wil2021_COI	DRWA-068	1	BE11616_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	GTAGTGGTAACTACTCATGCTTTA	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11616_Pvir_Dia2013_COI	DRWA-068	1	BE11616_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	CTTAGTGGCATTAATTCGDAATCC	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11616_Rhar_Sim2018_COI	DRWA-068	1	BE11616_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	CTGCTGCTATTGCTCATG	130.93016
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11618_Aamu_Bax2006_COI	DRWA-070	1	BE11618_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	GCACAACCGGGATCTTTACTTC	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11618_Pcan_Bot2011_IGS	DRWA-070	1	BE11618_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-070	IGS	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	CGTAATCCTCAGTACTGGCTA	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11618_Pper_Dia2013_COI	DRWA-070	1	BE11618_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	CTTAGTGGCATTAATTCGDAATCC	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11618_Sspa_Oph2007_COI	DRWA-070	1	BE11618_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	GAAGGCCTTCCGATGATC	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11618_Upin_Bot2011_COI	DRWA-070	1	BE11618_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	CTTTAATTACAGCGTTTTTATTGTTGT	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11618_Vgib_Wil2025_COI	DRWA-070	1	BE11618_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	CCCCTCTTTACAAGTTCTTTAGGGT	130.92862
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11619_Asen_Wil2025_COI	DRWA-071	1	BE11619_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	GTTGTTGTTACAACACATGCTCTTTTG	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11619_Cjap_Sim2018_COI	DRWA-071	1	BE11619_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11619_Esin_And2018_Cyb	DRWA-071	1	BE11619_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-071	Cytb	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	ACCCCTCCTCATATCCAACCA	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11619_Mgig_Bot2012_COI	DRWA-071	1	BE11619_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	TCTTATTCGTTGGAGACTTTATAACCCT	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11619_Mjap_Gib2022_COI	DRWA-071	1	BE11619_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	AATTTAATTGTGACTAGGCATGGTCTG	130.88164
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11621_Aamu_Bax2006_COI	DRWA-073	1	BE11621_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	GCACAACCGGGATCTTTACTTC	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11621_Pcan_Bot2011_IGS	DRWA-073	1	BE11621_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-073	IGS	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	CGTAATCCTCAGTACTGGCTA	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11621_Pper_Dia2013_COI	DRWA-073	1	BE11621_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	CTTAGTGGCATTAATTCGDAATCC	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11621_Sspa_Oph2007_COI	DRWA-073	1	BE11621_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	GAAGGCCTTCCGATGATC	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11621_Upin_Bot2011_COI	DRWA-073	1	BE11621_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	CTTTAATTACAGCGTTTTTATTGTTGT	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11621_Vgib_Wil2025_COI	DRWA-073	1	BE11621_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	CCCCTCTTTACAAGTTCTTTAGGGT	130.89041
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11622_Asen_Wil2025_COI	DRWA-074	1	BE11622_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	GTTGTTGTTACAACACATGCTCTTTTG	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11622_Cjap_Sim2018_COI	DRWA-074	1	BE11622_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11622_Esin_And2018_Cyb	DRWA-074	1	BE11622_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-074	Cytb	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	ACCCCTCCTCATATCCAACCA	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11622_Mgig_Bot2012_COI	DRWA-074	1	BE11622_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	TCTTATTCGTTGGAGACTTTATAACCCT	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11622_Mjap_Gib2022_COI	DRWA-074	1	BE11622_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	AATTTAATTGTGACTAGGCATGGTCTG	130.89165
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11623_Cmae_Bot2011_COI	DRWA-075	1	BE11623_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	ATGAACAGTCTATCCTCCTTTAG	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11623_Hsan_Wil2021_COI	DRWA-075	1	BE11623_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	CCTGGGCCGGTATAGTAGGT	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11623_Mros_Gib2022_COI	DRWA-075	1	BE11623_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11623_Msal_Bot2012_COI	DRWA-075	1	BE11623_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	GYTAGTTCCRATGATGTTAGCTG	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11623_Mstr_Wil2021_COI	DRWA-075	1	BE11623_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	GTAGTGGTAACTACTCATGCTTTA	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11623_Pvir_Dia2013_COI	DRWA-075	1	BE11623_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	CTTAGTGGCATTAATTCGDAATCC	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11623_Rhar_Sim2018_COI	DRWA-075	1	BE11623_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	CTGCTGCTATTGCTCATG	130.87952
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11625_Aamu_Bax2006_COI	DRWA-077	1	BE11625_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	GCACAACCGGGATCTTTACTTC	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11625_Pcan_Bot2011_IGS	DRWA-077	1	BE11625_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-077	IGS	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	CGTAATCCTCAGTACTGGCTA	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11625_Pper_Dia2013_COI	DRWA-077	1	BE11625_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	CTTAGTGGCATTAATTCGDAATCC	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11625_Sspa_Oph2007_COI	DRWA-077	1	BE11625_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	GAAGGCCTTCCGATGATC	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11625_Upin_Bot2011_COI	DRWA-077	1	BE11625_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	CTTTAATTACAGCGTTTTTATTGTTGT	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11625_Vgib_Wil2025_COI	DRWA-077	1	BE11625_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	CCCCTCTTTACAAGTTCTTTAGGGT	130.88243
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11626_Asen_Wil2025_COI	DRWA-078	1	BE11626_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	GTTGTTGTTACAACACATGCTCTTTTG	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11626_Cjap_Sim2018_COI	DRWA-078	1	BE11626_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11626_Esin_And2018_Cyb	DRWA-078	1	BE11626_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-078	Cytb	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	ACCCCTCCTCATATCCAACCA	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11626_Mgig_Bot2012_COI	DRWA-078	1	BE11626_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	TCTTATTCGTTGGAGACTTTATAACCCT	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11626_Mjap_Gib2022_COI	DRWA-078	1	BE11626_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	AATTTAATTGTGACTAGGCATGGTCTG	130.88409
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11627_Cmae_Bot2011_COI	DRWA-079	1	BE11627_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	ATGAACAGTCTATCCTCCTTTAG	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11627_Hsan_Wil2021_COI	DRWA-079	1	BE11627_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	CCTGGGCCGGTATAGTAGGT	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11627_Mros_Gib2022_COI	DRWA-079	1	BE11627_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11627_Msal_Bot2012_COI	DRWA-079	1	BE11627_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	GYTAGTTCCRATGATGTTAGCTG	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11627_Mstr_Wil2021_COI	DRWA-079	1	BE11627_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	GTAGTGGTAACTACTCATGCTTTA	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11627_Pvir_Dia2013_COI	DRWA-079	1	BE11627_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	CTTAGTGGCATTAATTCGDAATCC	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11627_Rhar_Sim2018_COI	DRWA-079	1	BE11627_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	CTGCTGCTATTGCTCATG	130.88853
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11629_Aamu_Bax2006_COI	DRWA-081	1	BE11629_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	GCACAACCGGGATCTTTACTTC	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11629_Pcan_Bot2011_IGS	DRWA-081	1	BE11629_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-081	IGS	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	CGTAATCCTCAGTACTGGCTA	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11629_Pper_Dia2013_COI	DRWA-081	1	BE11629_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	CTTAGTGGCATTAATTCGDAATCC	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11629_Sspa_Oph2007_COI	DRWA-081	1	BE11629_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	GAAGGCCTTCCGATGATC	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11629_Upin_Bot2011_COI	DRWA-081	1	BE11629_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	CTTTAATTACAGCGTTTTTATTGTTGT	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11629_Vgib_Wil2025_COI	DRWA-081	1	BE11629_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	CCCCTCTTTACAAGTTCTTTAGGGT	130.87884
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11630_Cmae_Bot2011_COI	DRWA-082	1	BE11630_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	ATGAACAGTCTATCCTCCTTTAG	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11630_Hsan_Wil2021_COI	DRWA-082	1	BE11630_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	CCTGGGCCGGTATAGTAGGT	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11630_Mros_Gib2022_COI	DRWA-082	1	BE11630_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11630_Msal_Bot2012_COI	DRWA-082	1	BE11630_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	GYTAGTTCCRATGATGTTAGCTG	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11630_Mstr_Wil2021_COI	DRWA-082	1	BE11630_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	GTAGTGGTAACTACTCATGCTTTA	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11630_Pvir_Dia2013_COI	DRWA-082	1	BE11630_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	CTTAGTGGCATTAATTCGDAATCC	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11630_Rhar_Sim2018_COI	DRWA-082	1	BE11630_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	CTGCTGCTATTGCTCATG	130.88028
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11632_Aamu_Bax2006_COI	DRWA-084	1	BE11632_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	GCACAACCGGGATCTTTACTTC	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11632_Pcan_Bot2011_IGS	DRWA-084	1	BE11632_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-084	IGS	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	CGTAATCCTCAGTACTGGCTA	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11632_Pper_Dia2013_COI	DRWA-084	1	BE11632_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	CTTAGTGGCATTAATTCGDAATCC	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11632_Sspa_Oph2007_COI	DRWA-084	1	BE11632_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	GAAGGCCTTCCGATGATC	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11632_Upin_Bot2011_COI	DRWA-084	1	BE11632_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	CTTTAATTACAGCGTTTTTATTGTTGT	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11632_Vgib_Wil2025_COI	DRWA-084	1	BE11632_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	CCCCTCTTTACAAGTTCTTTAGGGT	130.87667
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11633_Asen_Wil2025_COI	DRWA-085	1	BE11633_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	GTTGTTGTTACAACACATGCTCTTTTG	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11633_Cjap_Sim2018_COI	DRWA-085	1	BE11633_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11633_Esin_And2018_Cyb	DRWA-085	1	BE11633_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-085	Cytb	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	ACCCCTCCTCATATCCAACCA	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11633_Mgig_Bot2012_COI	DRWA-085	1	BE11633_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	TCTTATTCGTTGGAGACTTTATAACCCT	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11633_Mjap_Gib2022_COI	DRWA-085	1	BE11633_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	AATTTAATTGTGACTAGGCATGGTCTG	130.87809
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11634_Cmae_Bot2011_COI	DRWA-086	1	BE11634_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	ATGAACAGTCTATCCTCCTTTAG	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11634_Hsan_Wil2021_COI	DRWA-086	1	BE11634_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	CCTGGGCCGGTATAGTAGGT	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11634_Mros_Gib2022_COI	DRWA-086	1	BE11634_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11634_Msal_Bot2012_COI	DRWA-086	1	BE11634_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	GYTAGTTCCRATGATGTTAGCTG	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11634_Mstr_Wil2021_COI	DRWA-086	1	BE11634_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	GTAGTGGTAACTACTCATGCTTTA	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11634_Pvir_Dia2013_COI	DRWA-086	1	BE11634_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	CTTAGTGGCATTAATTCGDAATCC	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11634_Rhar_Sim2018_COI	DRWA-086	1	BE11634_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	CTGCTGCTATTGCTCATG	130.87958
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11636_Aamu_Bax2006_COI	DRWA-088	1	BE11636_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	GCACAACCGGGATCTTTACTTC	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11636_Pcan_Bot2011_IGS	DRWA-088	1	BE11636_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-088	IGS	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	CGTAATCCTCAGTACTGGCTA	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11636_Pper_Dia2013_COI	DRWA-088	1	BE11636_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	CTTAGTGGCATTAATTCGDAATCC	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11636_Sspa_Oph2007_COI	DRWA-088	1	BE11636_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	GAAGGCCTTCCGATGATC	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11636_Upin_Bot2011_COI	DRWA-088	1	BE11636_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	CTTTAATTACAGCGTTTTTATTGTTGT	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11636_Vgib_Wil2025_COI	DRWA-088	1	BE11636_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	CCCCTCTTTACAAGTTCTTTAGGGT	130.87464
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11637_Asen_Wil2025_COI	DRWA-089	1	BE11637_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	GTTGTTGTTACAACACATGCTCTTTTG	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11637_Cjap_Sim2018_COI	DRWA-089	1	BE11637_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11637_Esin_And2018_Cyb	DRWA-089	1	BE11637_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-089	Cytb	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	ACCCCTCCTCATATCCAACCA	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11637_Mgig_Bot2012_COI	DRWA-089	1	BE11637_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	TCTTATTCGTTGGAGACTTTATAACCCT	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11637_Mjap_Gib2022_COI	DRWA-089	1	BE11637_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	AATTTAATTGTGACTAGGCATGGTCTG	130.8761
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11638_Cmae_Bot2011_COI	DRWA-090	1	BE11638_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	ATGAACAGTCTATCCTCCTTTAG	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11638_Hsan_Wil2021_COI	DRWA-090	1	BE11638_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	CCTGGGCCGGTATAGTAGGT	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11638_Mros_Gib2022_COI	DRWA-090	1	BE11638_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11638_Msal_Bot2012_COI	DRWA-090	1	BE11638_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	GYTAGTTCCRATGATGTTAGCTG	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11638_Mstr_Wil2021_COI	DRWA-090	1	BE11638_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	GTAGTGGTAACTACTCATGCTTTA	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11638_Pvir_Dia2013_COI	DRWA-090	1	BE11638_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	CTTAGTGGCATTAATTCGDAATCC	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11638_Rhar_Sim2018_COI	DRWA-090	1	BE11638_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	CTGCTGCTATTGCTCATG	130.87752
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11640_Asen_Wil2025_COI	DRWA-092	1	BE11640_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	GTTGTTGTTACAACACATGCTCTTTTG	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11640_Cjap_Sim2018_COI	DRWA-092	1	BE11640_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11640_Esin_And2018_Cyb	DRWA-092	1	BE11640_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-092	Cytb	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	ACCCCTCCTCATATCCAACCA	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11640_Mgig_Bot2012_COI	DRWA-092	1	BE11640_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	TCTTATTCGTTGGAGACTTTATAACCCT	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11640_Mjap_Gib2022_COI	DRWA-092	1	BE11640_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	AATTTAATTGTGACTAGGCATGGTCTG	130.87405
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11641_Cmae_Bot2011_COI	DRWA-093	1	BE11641_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	ATGAACAGTCTATCCTCCTTTAG	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11641_Hsan_Wil2021_COI	DRWA-093	1	BE11641_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	CCTGGGCCGGTATAGTAGGT	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11641_Mros_Gib2022_COI	DRWA-093	1	BE11641_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11641_Msal_Bot2012_COI	DRWA-093	1	BE11641_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	GYTAGTTCCRATGATGTTAGCTG	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11641_Mstr_Wil2021_COI	DRWA-093	1	BE11641_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	GTAGTGGTAACTACTCATGCTTTA	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11641_Pvir_Dia2013_COI	DRWA-093	1	BE11641_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	CTTAGTGGCATTAATTCGDAATCC	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11641_Rhar_Sim2018_COI	DRWA-093	1	BE11641_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	CTGCTGCTATTGCTCATG	130.87532
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11643_Aamu_Bax2006_COI	DRWA-095	1	BE11643_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	GCACAACCGGGATCTTTACTTC	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11643_Pcan_Bot2011_IGS	DRWA-095	1	BE11643_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-095	IGS	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	CGTAATCCTCAGTACTGGCTA	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11643_Pper_Dia2013_COI	DRWA-095	1	BE11643_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	CTTAGTGGCATTAATTCGDAATCC	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11643_Sspa_Oph2007_COI	DRWA-095	1	BE11643_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	GAAGGCCTTCCGATGATC	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11643_Upin_Bot2011_COI	DRWA-095	1	BE11643_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	CTTTAATTACAGCGTTTTTATTGTTGT	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11643_Vgib_Wil2025_COI	DRWA-095	1	BE11643_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	CCCCTCTTTACAAGTTCTTTAGGGT	130.87313
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11644_Asen_Wil2025_COI	DRWA-096	1	BE11644_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	GTTGTTGTTACAACACATGCTCTTTTG	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11644_Cjap_Sim2018_COI	DRWA-096	1	BE11644_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11644_Esin_And2018_Cyb	DRWA-096	1	BE11644_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-096	Cytb	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	ACCCCTCCTCATATCCAACCA	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11644_Mgig_Bot2012_COI	DRWA-096	1	BE11644_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	TCTTATTCGTTGGAGACTTTATAACCCT	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11644_Mjap_Gib2022_COI	DRWA-096	1	BE11644_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	AATTTAATTGTGACTAGGCATGGTCTG	130.87454
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11645_Cmae_Bot2011_COI	DRWA-097	1	BE11645_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	ATGAACAGTCTATCCTCCTTTAG	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11645_Hsan_Wil2021_COI	DRWA-097	1	BE11645_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	CCTGGGCCGGTATAGTAGGT	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11645_Mros_Gib2022_COI	DRWA-097	1	BE11645_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11645_Msal_Bot2012_COI	DRWA-097	1	BE11645_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	GYTAGTTCCRATGATGTTAGCTG	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11645_Mstr_Wil2021_COI	DRWA-097	1	BE11645_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	GTAGTGGTAACTACTCATGCTTTA	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11645_Pvir_Dia2013_COI	DRWA-097	1	BE11645_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	CTTAGTGGCATTAATTCGDAATCC	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11645_Rhar_Sim2018_COI	DRWA-097	1	BE11645_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	CTGCTGCTATTGCTCATG	130.87592
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11647_Aamu_Bax2006_COI	DRWA-099	1	BE11647_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	GCACAACCGGGATCTTTACTTC	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11647_Pcan_Bot2011_IGS	DRWA-099	1	BE11647_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-099	IGS	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	CGTAATCCTCAGTACTGGCTA	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11647_Pper_Dia2013_COI	DRWA-099	1	BE11647_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	CTTAGTGGCATTAATTCGDAATCC	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11647_Sspa_Oph2007_COI	DRWA-099	1	BE11647_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	GAAGGCCTTCCGATGATC	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11647_Upin_Bot2011_COI	DRWA-099	1	BE11647_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	CTTTAATTACAGCGTTTTTATTGTTGT	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11647_Vgib_Wil2025_COI	DRWA-099	1	BE11647_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	CCCCTCTTTACAAGTTCTTTAGGGT	130.87343
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11648_Asen_Wil2025_COI	DRWA-100	1	BE11648_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	GTTGTTGTTACAACACATGCTCTTTTG	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11648_Cjap_Sim2018_COI	DRWA-100	1	BE11648_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11648_Esin_And2018_Cyb	DRWA-100	1	BE11648_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-100	Cytb	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	ACCCCTCCTCATATCCAACCA	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11648_Mgig_Bot2012_COI	DRWA-100	1	BE11648_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	TCTTATTCGTTGGAGACTTTATAACCCT	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11648_Mjap_Gib2022_COI	DRWA-100	1	BE11648_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	AATTTAATTGTGACTAGGCATGGTCTG	130.87492
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11649_Cmae_Bot2011_COI	DRWA-101	1	BE11649_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	ATGAACAGTCTATCCTCCTTTAG	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11649_Hsan_Wil2021_COI	DRWA-101	1	BE11649_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	CCTGGGCCGGTATAGTAGGT	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11649_Mros_Gib2022_COI	DRWA-101	1	BE11649_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11649_Msal_Bot2012_COI	DRWA-101	1	BE11649_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	GYTAGTTCCRATGATGTTAGCTG	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11649_Mstr_Wil2021_COI	DRWA-101	1	BE11649_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	GTAGTGGTAACTACTCATGCTTTA	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11649_Pvir_Dia2013_COI	DRWA-101	1	BE11649_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	CTTAGTGGCATTAATTCGDAATCC	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11649_Rhar_Sim2018_COI	DRWA-101	1	BE11649_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	CTGCTGCTATTGCTCATG	130.87622
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11650_Aamu_Bax2006_COI	DRWA-102	1	BE11650_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	GCACAACCGGGATCTTTACTTC	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11650_Pcan_Bot2011_IGS	DRWA-102	1	BE11650_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-102	IGS	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	CGTAATCCTCAGTACTGGCTA	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11650_Pper_Dia2013_COI	DRWA-102	1	BE11650_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	CTTAGTGGCATTAATTCGDAATCC	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11650_Sspa_Oph2007_COI	DRWA-102	1	BE11650_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	GAAGGCCTTCCGATGATC	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11650_Upin_Bot2011_COI	DRWA-102	1	BE11650_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	CTTTAATTACAGCGTTTTTATTGTTGT	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11650_Vgib_Wil2025_COI	DRWA-102	1	BE11650_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	CCCCTCTTTACAAGTTCTTTAGGGT	130.87416
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11651_Asen_Wil2025_COI	DRWA-103	1	BE11651_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	GTTGTTGTTACAACACATGCTCTTTTG	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11651_Cjap_Sim2018_COI	DRWA-103	1	BE11651_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11651_Esin_And2018_Cyb	DRWA-103	1	BE11651_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-103	Cytb	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	ACCCCTCCTCATATCCAACCA	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11651_Mgig_Bot2012_COI	DRWA-103	1	BE11651_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	TCTTATTCGTTGGAGACTTTATAACCCT	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11651_Mjap_Gib2022_COI	DRWA-103	1	BE11651_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	AATTTAATTGTGACTAGGCATGGTCTG	130.87559
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11652_Cmae_Bot2011_COI	DRWA-104	1	BE11652_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	ATGAACAGTCTATCCTCCTTTAG	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11652_Hsan_Wil2021_COI	DRWA-104	1	BE11652_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	CCTGGGCCGGTATAGTAGGT	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11652_Mros_Gib2022_COI	DRWA-104	1	BE11652_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11652_Msal_Bot2012_COI	DRWA-104	1	BE11652_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	GYTAGTTCCRATGATGTTAGCTG	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11652_Mstr_Wil2021_COI	DRWA-104	1	BE11652_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	GTAGTGGTAACTACTCATGCTTTA	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11652_Pvir_Dia2013_COI	DRWA-104	1	BE11652_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	CTTAGTGGCATTAATTCGDAATCC	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11652_Rhar_Sim2018_COI	DRWA-104	1	BE11652_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	CTGCTGCTATTGCTCATG	130.87494
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11797_Aamu_Bax2006_COI	DRWB-100	1	BE11797_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	GCACAACCGGGATCTTTACTTC	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11797_Pcan_Bot2011_IGS	DRWB-100	1	BE11797_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-100	IGS	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	CGTAATCCTCAGTACTGGCTA	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11797_Pper_Dia2013_COI	DRWB-100	1	BE11797_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	CTTAGTGGCATTAATTCGDAATCC	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11797_Sspa_Oph2007_COI	DRWB-100	1	BE11797_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	GAAGGCCTTCCGATGATC	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11797_Upin_Bot2011_COI	DRWB-100	1	BE11797_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	CTTTAATTACAGCGTTTTTATTGTTGT	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11797_Vgib_Wil2025_COI	DRWB-100	1	BE11797_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	CCCCTCTTTACAAGTTCTTTAGGGT	130.92721
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11798_Asen_Wil2025_COI	DRWB-101	1	BE11798_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	GTTGTTGTTACAACACATGCTCTTTTG	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11798_Cjap_Sim2018_COI	DRWB-101	1	BE11798_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11798_Esin_And2018_Cyb	DRWB-101	1	BE11798_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-101	Cytb	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	ACCCCTCCTCATATCCAACCA	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11798_Mgig_Bot2012_COI	DRWB-101	1	BE11798_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	TCTTATTCGTTGGAGACTTTATAACCCT	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11798_Mjap_Gib2022_COI	DRWB-101	1	BE11798_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	AATTTAATTGTGACTAGGCATGGTCTG	130.9282
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11799_Cmae_Bot2011_COI	DRWB-102	1	BE11799_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	ATGAACAGTCTATCCTCCTTTAG	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11799_Hsan_Wil2021_COI	DRWB-102	1	BE11799_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	CCTGGGCCGGTATAGTAGGT	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11799_Mros_Gib2022_COI	DRWB-102	1	BE11799_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11799_Msal_Bot2012_COI	DRWB-102	1	BE11799_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	GYTAGTTCCRATGATGTTAGCTG	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11799_Mstr_Wil2021_COI	DRWB-102	1	BE11799_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	GTAGTGGTAACTACTCATGCTTTA	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11799_Pvir_Dia2013_COI	DRWB-102	1	BE11799_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	CTTAGTGGCATTAATTCGDAATCC	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11799_Rhar_Sim2018_COI	DRWB-102	1	BE11799_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	CTGCTGCTATTGCTCATG	130.9292
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11801_Aamu_Bax2006_COI	DRWB-104	1	BE11801_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	GCACAACCGGGATCTTTACTTC	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11801_Pcan_Bot2011_IGS	DRWB-104	1	BE11801_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-104	IGS	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	CGTAATCCTCAGTACTGGCTA	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11801_Pper_Dia2013_COI	DRWB-104	1	BE11801_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	CTTAGTGGCATTAATTCGDAATCC	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11801_Sspa_Oph2007_COI	DRWB-104	1	BE11801_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	GAAGGCCTTCCGATGATC	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11801_Upin_Bot2011_COI	DRWB-104	1	BE11801_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	CTTTAATTACAGCGTTTTTATTGTTGT	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11801_Vgib_Wil2025_COI	DRWB-104	1	BE11801_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	CCCCTCTTTACAAGTTCTTTAGGGT	130.92768
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11802_Asen_Wil2025_COI	DRWB-105	1	BE11802_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	GTTGTTGTTACAACACATGCTCTTTTG	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11802_Cjap_Sim2018_COI	DRWB-105	1	BE11802_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11802_Esin_And2018_Cyb	DRWB-105	1	BE11802_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-105	Cytb	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	ACCCCTCCTCATATCCAACCA	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11802_Mgig_Bot2012_COI	DRWB-105	1	BE11802_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	TCTTATTCGTTGGAGACTTTATAACCCT	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11802_Mjap_Gib2022_COI	DRWB-105	1	BE11802_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	AATTTAATTGTGACTAGGCATGGTCTG	130.92863
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11803_Cmae_Bot2011_COI	DRWB-106	1	BE11803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	ATGAACAGTCTATCCTCCTTTAG	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11803_Hsan_Wil2021_COI	DRWB-106	1	BE11803_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	CCTGGGCCGGTATAGTAGGT	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11803_Mros_Gib2022_COI	DRWB-106	1	BE11803_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11803_Msal_Bot2012_COI	DRWB-106	1	BE11803_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	GYTAGTTCCRATGATGTTAGCTG	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11803_Mstr_Wil2021_COI	DRWB-106	1	BE11803_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	GTAGTGGTAACTACTCATGCTTTA	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11803_Pvir_Dia2013_COI	DRWB-106	1	BE11803_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	CTTAGTGGCATTAATTCGDAATCC	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11803_Rhar_Sim2018_COI	DRWB-106	1	BE11803_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	CTGCTGCTATTGCTCATG	130.88186
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11805_Aamu_Bax2006_COI	DRWB-108	1	BE11805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	GCACAACCGGGATCTTTACTTC	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11805_Pcan_Bot2011_IGS	DRWB-108	1	BE11805_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-108	IGS	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	CGTAATCCTCAGTACTGGCTA	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11805_Pper_Dia2013_COI	DRWB-108	1	BE11805_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	CTTAGTGGCATTAATTCGDAATCC	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11805_Sspa_Oph2007_COI	DRWB-108	1	BE11805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	GAAGGCCTTCCGATGATC	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11805_Upin_Bot2011_COI	DRWB-108	1	BE11805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	CTTTAATTACAGCGTTTTTATTGTTGT	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11805_Vgib_Wil2025_COI	DRWB-108	1	BE11805_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	CCCCTCTTTACAAGTTCTTTAGGGT	130.89031
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11806_Asen_Wil2025_COI	DRWB-109	1	BE11806_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	GTTGTTGTTACAACACATGCTCTTTTG	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11806_Cjap_Sim2018_COI	DRWB-109	1	BE11806_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11806_Esin_And2018_Cyb	DRWB-109	1	BE11806_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-109	Cytb	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	ACCCCTCCTCATATCCAACCA	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11806_Mgig_Bot2012_COI	DRWB-109	1	BE11806_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	TCTTATTCGTTGGAGACTTTATAACCCT	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11806_Mjap_Gib2022_COI	DRWB-109	1	BE11806_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	AATTTAATTGTGACTAGGCATGGTCTG	130.89198
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11807_Cmae_Bot2011_COI	DRWB-110	1	BE11807_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	ATGAACAGTCTATCCTCCTTTAG	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11807_Hsan_Wil2021_COI	DRWB-110	1	BE11807_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	CCTGGGCCGGTATAGTAGGT	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11807_Mros_Gib2022_COI	DRWB-110	1	BE11807_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11807_Msal_Bot2012_COI	DRWB-110	1	BE11807_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	GYTAGTTCCRATGATGTTAGCTG	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11807_Mstr_Wil2021_COI	DRWB-110	1	BE11807_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	GTAGTGGTAACTACTCATGCTTTA	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11807_Pvir_Dia2013_COI	DRWB-110	1	BE11807_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	CTTAGTGGCATTAATTCGDAATCC	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11807_Rhar_Sim2018_COI	DRWB-110	1	BE11807_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	CTGCTGCTATTGCTCATG	130.87946
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11809_Aamu_Bax2006_COI	DRWB-112	1	BE11809_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	GCACAACCGGGATCTTTACTTC	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11809_Pcan_Bot2011_IGS	DRWB-112	1	BE11809_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-112	IGS	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	CGTAATCCTCAGTACTGGCTA	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11809_Pper_Dia2013_COI	DRWB-112	1	BE11809_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	CTTAGTGGCATTAATTCGDAATCC	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11809_Sspa_Oph2007_COI	DRWB-112	1	BE11809_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	GAAGGCCTTCCGATGATC	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11809_Upin_Bot2011_COI	DRWB-112	1	BE11809_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	CTTTAATTACAGCGTTTTTATTGTTGT	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11809_Vgib_Wil2025_COI	DRWB-112	1	BE11809_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	CCCCTCTTTACAAGTTCTTTAGGGT	130.88251
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11810_Cmae_Bot2011_COI	DRWB-113	1	BE11810_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	ATGAACAGTCTATCCTCCTTTAG	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11810_Hsan_Wil2021_COI	DRWB-113	1	BE11810_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	CCTGGGCCGGTATAGTAGGT	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11810_Mros_Gib2022_COI	DRWB-113	1	BE11810_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11810_Msal_Bot2012_COI	DRWB-113	1	BE11810_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	GYTAGTTCCRATGATGTTAGCTG	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11810_Mstr_Wil2021_COI	DRWB-113	1	BE11810_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	GTAGTGGTAACTACTCATGCTTTA	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11810_Pvir_Dia2013_COI	DRWB-113	1	BE11810_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	CTTAGTGGCATTAATTCGDAATCC	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11810_Rhar_Sim2018_COI	DRWB-113	1	BE11810_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	CTGCTGCTATTGCTCATG	130.88405
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11812_Aamu_Bax2006_COI	DRWB-115	1	BE11812_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	GCACAACCGGGATCTTTACTTC	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11812_Pcan_Bot2011_IGS	DRWB-115	1	BE11812_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-115	IGS	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	CGTAATCCTCAGTACTGGCTA	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11812_Pper_Dia2013_COI	DRWB-115	1	BE11812_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	CTTAGTGGCATTAATTCGDAATCC	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11812_Sspa_Oph2007_COI	DRWB-115	1	BE11812_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	GAAGGCCTTCCGATGATC	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11812_Upin_Bot2011_COI	DRWB-115	1	BE11812_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	CTTTAATTACAGCGTTTTTATTGTTGT	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11812_Vgib_Wil2025_COI	DRWB-115	1	BE11812_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	CCCCTCTTTACAAGTTCTTTAGGGT	130.88975
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11813_Asen_Wil2025_COI	DRWB-116	1	BE11813_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	GTTGTTGTTACAACACATGCTCTTTTG	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11813_Cjap_Sim2018_COI	DRWB-116	1	BE11813_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11813_Esin_And2018_Cyb	DRWB-116	1	BE11813_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-116	Cytb	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	ACCCCTCCTCATATCCAACCA	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11813_Mgig_Bot2012_COI	DRWB-116	1	BE11813_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	TCTTATTCGTTGGAGACTTTATAACCCT	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11813_Mjap_Gib2022_COI	DRWB-116	1	BE11813_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	AATTTAATTGTGACTAGGCATGGTCTG	130.87898
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11814_Cmae_Bot2011_COI	DRWB-117	1	BE11814_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	ATGAACAGTCTATCCTCCTTTAG	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11814_Hsan_Wil2021_COI	DRWB-117	1	BE11814_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	CCTGGGCCGGTATAGTAGGT	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11814_Mros_Gib2022_COI	DRWB-117	1	BE11814_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11814_Msal_Bot2012_COI	DRWB-117	1	BE11814_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	GYTAGTTCCRATGATGTTAGCTG	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11814_Mstr_Wil2021_COI	DRWB-117	1	BE11814_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	GTAGTGGTAACTACTCATGCTTTA	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11814_Pvir_Dia2013_COI	DRWB-117	1	BE11814_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	CTTAGTGGCATTAATTCGDAATCC	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11814_Rhar_Sim2018_COI	DRWB-117	1	BE11814_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	CTGCTGCTATTGCTCATG	130.88021
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11816_Aamu_Bax2006_COI	DRWB-119	1	BE11816_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	GCACAACCGGGATCTTTACTTC	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11816_Pcan_Bot2011_IGS	DRWB-119	1	BE11816_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-119	IGS	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	CGTAATCCTCAGTACTGGCTA	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11816_Pper_Dia2013_COI	DRWB-119	1	BE11816_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	CTTAGTGGCATTAATTCGDAATCC	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11816_Sspa_Oph2007_COI	DRWB-119	1	BE11816_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	GAAGGCCTTCCGATGATC	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11816_Upin_Bot2011_COI	DRWB-119	1	BE11816_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	CTTTAATTACAGCGTTTTTATTGTTGT	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11816_Vgib_Wil2025_COI	DRWB-119	1	BE11816_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	CCCCTCTTTACAAGTTCTTTAGGGT	130.87668
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11817_Asen_Wil2025_COI	DRWB-120	1	BE11817_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	GTTGTTGTTACAACACATGCTCTTTTG	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11817_Cjap_Sim2018_COI	DRWB-120	1	BE11817_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11817_Esin_And2018_Cyb	DRWB-120	1	BE11817_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-120	Cytb	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	ACCCCTCCTCATATCCAACCA	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11817_Mgig_Bot2012_COI	DRWB-120	1	BE11817_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	TCTTATTCGTTGGAGACTTTATAACCCT	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11817_Mjap_Gib2022_COI	DRWB-120	1	BE11817_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	AATTTAATTGTGACTAGGCATGGTCTG	130.87813
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11818_Cmae_Bot2011_COI	DRWB-121	1	BE11818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	ATGAACAGTCTATCCTCCTTTAG	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11818_Hsan_Wil2021_COI	DRWB-121	1	BE11818_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	CCTGGGCCGGTATAGTAGGT	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11818_Mros_Gib2022_COI	DRWB-121	1	BE11818_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11818_Msal_Bot2012_COI	DRWB-121	1	BE11818_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	GYTAGTTCCRATGATGTTAGCTG	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11818_Mstr_Wil2021_COI	DRWB-121	1	BE11818_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	GTAGTGGTAACTACTCATGCTTTA	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11818_Pvir_Dia2013_COI	DRWB-121	1	BE11818_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	CTTAGTGGCATTAATTCGDAATCC	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11818_Rhar_Sim2018_COI	DRWB-121	1	BE11818_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	CTGCTGCTATTGCTCATG	130.87964
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11820_Asen_Wil2025_COI	DRWB-123	1	BE11820_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	GTTGTTGTTACAACACATGCTCTTTTG	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11820_Cjap_Sim2018_COI	DRWB-123	1	BE11820_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11820_Esin_And2018_Cyb	DRWB-123	1	BE11820_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-123	Cytb	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	ACCCCTCCTCATATCCAACCA	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11820_Mgig_Bot2012_COI	DRWB-123	1	BE11820_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	TCTTATTCGTTGGAGACTTTATAACCCT	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11820_Mjap_Gib2022_COI	DRWB-123	1	BE11820_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	AATTTAATTGTGACTAGGCATGGTCTG	130.87459
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11821_Cmae_Bot2011_COI	DRWB-124	1	BE11821_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	ATGAACAGTCTATCCTCCTTTAG	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11821_Hsan_Wil2021_COI	DRWB-124	1	BE11821_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	CCTGGGCCGGTATAGTAGGT	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11821_Mros_Gib2022_COI	DRWB-124	1	BE11821_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11821_Msal_Bot2012_COI	DRWB-124	1	BE11821_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	GYTAGTTCCRATGATGTTAGCTG	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11821_Mstr_Wil2021_COI	DRWB-124	1	BE11821_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	GTAGTGGTAACTACTCATGCTTTA	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11821_Pvir_Dia2013_COI	DRWB-124	1	BE11821_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	CTTAGTGGCATTAATTCGDAATCC	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11821_Rhar_Sim2018_COI	DRWB-124	1	BE11821_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	CTGCTGCTATTGCTCATG	130.87608
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11823_Aamu_Bax2006_COI	DRWB-126	1	BE11823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	GCACAACCGGGATCTTTACTTC	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11823_Pcan_Bot2011_IGS	DRWB-126	1	BE11823_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-126	IGS	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	CGTAATCCTCAGTACTGGCTA	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11823_Pper_Dia2013_COI	DRWB-126	1	BE11823_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	CTTAGTGGCATTAATTCGDAATCC	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11823_Sspa_Oph2007_COI	DRWB-126	1	BE11823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	GAAGGCCTTCCGATGATC	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11823_Upin_Bot2011_COI	DRWB-126	1	BE11823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	CTTTAATTACAGCGTTTTTATTGTTGT	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11823_Vgib_Wil2025_COI	DRWB-126	1	BE11823_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	CCCCTCTTTACAAGTTCTTTAGGGT	130.87892
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11824_Asen_Wil2025_COI	DRWB-127	1	BE11824_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	GTTGTTGTTACAACACATGCTCTTTTG	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11824_Cjap_Sim2018_COI	DRWB-127	1	BE11824_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11824_Esin_And2018_Cyb	DRWB-127	1	BE11824_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-127	Cytb	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	ACCCCTCCTCATATCCAACCA	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11824_Mgig_Bot2012_COI	DRWB-127	1	BE11824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	TCTTATTCGTTGGAGACTTTATAACCCT	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11824_Mjap_Gib2022_COI	DRWB-127	1	BE11824_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	AATTTAATTGTGACTAGGCATGGTCTG	130.87409
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11825_Cmae_Bot2011_COI	DRWB-128	1	BE11825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	ATGAACAGTCTATCCTCCTTTAG	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11825_Hsan_Wil2021_COI	DRWB-128	1	BE11825_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	CCTGGGCCGGTATAGTAGGT	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11825_Mros_Gib2022_COI	DRWB-128	1	BE11825_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11825_Msal_Bot2012_COI	DRWB-128	1	BE11825_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	GYTAGTTCCRATGATGTTAGCTG	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11825_Mstr_Wil2021_COI	DRWB-128	1	BE11825_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	GTAGTGGTAACTACTCATGCTTTA	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11825_Pvir_Dia2013_COI	DRWB-128	1	BE11825_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	CTTAGTGGCATTAATTCGDAATCC	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11825_Rhar_Sim2018_COI	DRWB-128	1	BE11825_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	CTGCTGCTATTGCTCATG	130.87545
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11827_Aamu_Bax2006_COI	DRWB-130	1	BE11827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	GCACAACCGGGATCTTTACTTC	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11827_Pcan_Bot2011_IGS	DRWB-130	1	BE11827_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-130	IGS	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	CGTAATCCTCAGTACTGGCTA	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11827_Pper_Dia2013_COI	DRWB-130	1	BE11827_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	CTTAGTGGCATTAATTCGDAATCC	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11827_Sspa_Oph2007_COI	DRWB-130	1	BE11827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	GAAGGCCTTCCGATGATC	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11827_Upin_Bot2011_COI	DRWB-130	1	BE11827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	CTTTAATTACAGCGTTTTTATTGTTGT	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11827_Vgib_Wil2025_COI	DRWB-130	1	BE11827_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	CCCCTCTTTACAAGTTCTTTAGGGT	130.87335
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11828_Asen_Wil2025_COI	DRWB-131	1	BE11828_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	GTTGTTGTTACAACACATGCTCTTTTG	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11828_Cjap_Sim2018_COI	DRWB-131	1	BE11828_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11828_Esin_And2018_Cyb	DRWB-131	1	BE11828_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-131	Cytb	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	ACCCCTCCTCATATCCAACCA	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11828_Mgig_Bot2012_COI	DRWB-131	1	BE11828_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	TCTTATTCGTTGGAGACTTTATAACCCT	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11828_Mjap_Gib2022_COI	DRWB-131	1	BE11828_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	AATTTAATTGTGACTAGGCATGGTCTG	130.87468
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11829_Cmae_Bot2011_COI	DRWB-132	1	BE11829_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	ATGAACAGTCTATCCTCCTTTAG	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11829_Hsan_Wil2021_COI	DRWB-132	1	BE11829_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	CCTGGGCCGGTATAGTAGGT	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11829_Mros_Gib2022_COI	DRWB-132	1	BE11829_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11829_Msal_Bot2012_COI	DRWB-132	1	BE11829_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	GYTAGTTCCRATGATGTTAGCTG	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11829_Mstr_Wil2021_COI	DRWB-132	1	BE11829_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	GTAGTGGTAACTACTCATGCTTTA	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11829_Pvir_Dia2013_COI	DRWB-132	1	BE11829_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	CTTAGTGGCATTAATTCGDAATCC	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11829_Rhar_Sim2018_COI	DRWB-132	1	BE11829_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	CTGCTGCTATTGCTCATG	130.87609
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11830_Aamu_Bax2006_COI	DRWB-133	1	BE11830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	GCACAACCGGGATCTTTACTTC	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11830_Pcan_Bot2011_IGS	DRWB-133	1	BE11830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-133	IGS	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	CGTAATCCTCAGTACTGGCTA	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11830_Pper_Dia2013_COI	DRWB-133	1	BE11830_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	CTTAGTGGCATTAATTCGDAATCC	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11830_Sspa_Oph2007_COI	DRWB-133	1	BE11830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	GAAGGCCTTCCGATGATC	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11830_Upin_Bot2011_COI	DRWB-133	1	BE11830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	CTTTAATTACAGCGTTTTTATTGTTGT	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11830_Vgib_Wil2025_COI	DRWB-133	1	BE11830_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	CCCCTCTTTACAAGTTCTTTAGGGT	130.87392
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11831_Asen_Wil2025_COI	DRWB-134	1	BE11831_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	GTTGTTGTTACAACACATGCTCTTTTG	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11831_Cjap_Sim2018_COI	DRWB-134	1	BE11831_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11831_Esin_And2018_Cyb	DRWB-134	1	BE11831_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-134	Cytb	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	ACCCCTCCTCATATCCAACCA	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11831_Mgig_Bot2012_COI	DRWB-134	1	BE11831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	TCTTATTCGTTGGAGACTTTATAACCCT	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11831_Mjap_Gib2022_COI	DRWB-134	1	BE11831_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	AATTTAATTGTGACTAGGCATGGTCTG	130.87337
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11832_Cmae_Bot2011_COI	DRWB-135	1	BE11832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	ATGAACAGTCTATCCTCCTTTAG	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11832_Hsan_Wil2021_COI	DRWB-135	1	BE11832_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	CCTGGGCCGGTATAGTAGGT	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11832_Mros_Gib2022_COI	DRWB-135	1	BE11832_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11832_Msal_Bot2012_COI	DRWB-135	1	BE11832_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	GYTAGTTCCRATGATGTTAGCTG	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11832_Mstr_Wil2021_COI	DRWB-135	1	BE11832_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	GTAGTGGTAACTACTCATGCTTTA	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11832_Pvir_Dia2013_COI	DRWB-135	1	BE11832_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	CTTAGTGGCATTAATTCGDAATCC	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11832_Rhar_Sim2018_COI	DRWB-135	1	BE11832_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	CTGCTGCTATTGCTCATG	130.87487
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11876_Cmae_Bot2011_COI	DRWB-071	1	BE11876_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	ATGAACAGTCTATCCTCCTTTAG	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11876_Hsan_Wil2021_COI	DRWB-071	1	BE11876_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	CCTGGGCCGGTATAGTAGGT	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11876_Mros_Gib2022_COI	DRWB-071	1	BE11876_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11876_Msal_Bot2012_COI	DRWB-071	1	BE11876_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	GYTAGTTCCRATGATGTTAGCTG	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11876_Mstr_Wil2021_COI	DRWB-071	1	BE11876_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	GTAGTGGTAACTACTCATGCTTTA	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11876_Pvir_Dia2013_COI	DRWB-071	1	BE11876_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	CTTAGTGGCATTAATTCGDAATCC	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11876_Rhar_Sim2018_COI	DRWB-071	1	BE11876_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	CTGCTGCTATTGCTCATG	130.93133
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11878_Aamu_Bax2006_COI	DRWB-073	1	BE11878_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	GCACAACCGGGATCTTTACTTC	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11878_Pcan_Bot2011_IGS	DRWB-073	1	BE11878_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-073	IGS	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	CGTAATCCTCAGTACTGGCTA	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11878_Pper_Dia2013_COI	DRWB-073	1	BE11878_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	CTTAGTGGCATTAATTCGDAATCC	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11878_Sspa_Oph2007_COI	DRWB-073	1	BE11878_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	GAAGGCCTTCCGATGATC	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11878_Upin_Bot2011_COI	DRWB-073	1	BE11878_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	CTTTAATTACAGCGTTTTTATTGTTGT	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11878_Vgib_Wil2025_COI	DRWB-073	1	BE11878_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	CCCCTCTTTACAAGTTCTTTAGGGT	130.93104
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11879_Asen_Wil2025_COI	DRWB-074	1	BE11879_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	GTTGTTGTTACAACACATGCTCTTTTG	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11879_Cjap_Sim2018_COI	DRWB-074	1	BE11879_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11879_Esin_And2018_Cyb	DRWB-074	1	BE11879_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-074	Cytb	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	ACCCCTCCTCATATCCAACCA	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11879_Mgig_Bot2012_COI	DRWB-074	1	BE11879_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	TCTTATTCGTTGGAGACTTTATAACCCT	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11879_Mjap_Gib2022_COI	DRWB-074	1	BE11879_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	AATTTAATTGTGACTAGGCATGGTCTG	130.93201
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11881_Aamu_Bax2006_COI	DRWB-076	1	BE11881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	GCACAACCGGGATCTTTACTTC	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11881_Pcan_Bot2011_IGS	DRWB-076	1	BE11881_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-076	IGS	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	CGTAATCCTCAGTACTGGCTA	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11881_Pper_Dia2013_COI	DRWB-076	1	BE11881_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	CTTAGTGGCATTAATTCGDAATCC	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11881_Sspa_Oph2007_COI	DRWB-076	1	BE11881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	GAAGGCCTTCCGATGATC	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11881_Upin_Bot2011_COI	DRWB-076	1	BE11881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	CTTTAATTACAGCGTTTTTATTGTTGT	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11881_Vgib_Wil2025_COI	DRWB-076	1	BE11881_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	CCCCTCTTTACAAGTTCTTTAGGGT	130.93156
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11882_Asen_Wil2025_COI	DRWB-077	1	BE11882_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	GTTGTTGTTACAACACATGCTCTTTTG	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11882_Cjap_Sim2018_COI	DRWB-077	1	BE11882_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11882_Esin_And2018_Cyb	DRWB-077	1	BE11882_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-077	Cytb	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	ACCCCTCCTCATATCCAACCA	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11882_Mgig_Bot2012_COI	DRWB-077	1	BE11882_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	TCTTATTCGTTGGAGACTTTATAACCCT	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11882_Mjap_Gib2022_COI	DRWB-077	1	BE11882_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	AATTTAATTGTGACTAGGCATGGTCTG	130.93254
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11883_Cmae_Bot2011_COI	DRWB-078	1	BE11883_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	ATGAACAGTCTATCCTCCTTTAG	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11883_Hsan_Wil2021_COI	DRWB-078	1	BE11883_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	CCTGGGCCGGTATAGTAGGT	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11883_Mros_Gib2022_COI	DRWB-078	1	BE11883_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11883_Msal_Bot2012_COI	DRWB-078	1	BE11883_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	GYTAGTTCCRATGATGTTAGCTG	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11883_Mstr_Wil2021_COI	DRWB-078	1	BE11883_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	GTAGTGGTAACTACTCATGCTTTA	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11883_Pvir_Dia2013_COI	DRWB-078	1	BE11883_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	CTTAGTGGCATTAATTCGDAATCC	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11883_Rhar_Sim2018_COI	DRWB-078	1	BE11883_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	CTGCTGCTATTGCTCATG	130.9301
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11885_Aamu_Bax2006_COI	DRWB-080	1	BE11885_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	GCACAACCGGGATCTTTACTTC	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11885_Pcan_Bot2011_IGS	DRWB-080	1	BE11885_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-080	IGS	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	CGTAATCCTCAGTACTGGCTA	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11885_Pper_Dia2013_COI	DRWB-080	1	BE11885_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	CTTAGTGGCATTAATTCGDAATCC	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11885_Sspa_Oph2007_COI	DRWB-080	1	BE11885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	GAAGGCCTTCCGATGATC	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11885_Upin_Bot2011_COI	DRWB-080	1	BE11885_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	CTTTAATTACAGCGTTTTTATTGTTGT	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11885_Vgib_Wil2025_COI	DRWB-080	1	BE11885_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	CCCCTCTTTACAAGTTCTTTAGGGT	130.93206
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11886_Asen_Wil2025_COI	DRWB-081	1	BE11886_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	GTTGTTGTTACAACACATGCTCTTTTG	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11886_Cjap_Sim2018_COI	DRWB-081	1	BE11886_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11886_Esin_And2018_Cyb	DRWB-081	1	BE11886_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-081	Cytb	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	ACCCCTCCTCATATCCAACCA	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11886_Mgig_Bot2012_COI	DRWB-081	1	BE11886_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	TCTTATTCGTTGGAGACTTTATAACCCT	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11886_Mjap_Gib2022_COI	DRWB-081	1	BE11886_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	AATTTAATTGTGACTAGGCATGGTCTG	130.92961
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11887_Cmae_Bot2011_COI	DRWB-082	1	BE11887_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	ATGAACAGTCTATCCTCCTTTAG	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11887_Hsan_Wil2021_COI	DRWB-082	1	BE11887_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	CCTGGGCCGGTATAGTAGGT	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11887_Mros_Gib2022_COI	DRWB-082	1	BE11887_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11887_Msal_Bot2012_COI	DRWB-082	1	BE11887_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	GYTAGTTCCRATGATGTTAGCTG	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11887_Mstr_Wil2021_COI	DRWB-082	1	BE11887_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	GTAGTGGTAACTACTCATGCTTTA	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11887_Pvir_Dia2013_COI	DRWB-082	1	BE11887_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	CTTAGTGGCATTAATTCGDAATCC	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11887_Rhar_Sim2018_COI	DRWB-082	1	BE11887_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	CTGCTGCTATTGCTCATG	130.93061
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11889_Aamu_Bax2006_COI	DRWB-084	1	BE11889_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	GCACAACCGGGATCTTTACTTC	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11889_Pcan_Bot2011_IGS	DRWB-084	1	BE11889_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-084	IGS	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	CGTAATCCTCAGTACTGGCTA	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11889_Pper_Dia2013_COI	DRWB-084	1	BE11889_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	CTTAGTGGCATTAATTCGDAATCC	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11889_Sspa_Oph2007_COI	DRWB-084	1	BE11889_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	GAAGGCCTTCCGATGATC	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11889_Upin_Bot2011_COI	DRWB-084	1	BE11889_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	CTTTAATTACAGCGTTTTTATTGTTGT	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11889_Vgib_Wil2025_COI	DRWB-084	1	BE11889_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	CCCCTCTTTACAAGTTCTTTAGGGT	130.92909
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11890_Cmae_Bot2011_COI	DRWB-085	1	BE11890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	ATGAACAGTCTATCCTCCTTTAG	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11890_Hsan_Wil2021_COI	DRWB-085	1	BE11890_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	CCTGGGCCGGTATAGTAGGT	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11890_Mros_Gib2022_COI	DRWB-085	1	BE11890_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11890_Msal_Bot2012_COI	DRWB-085	1	BE11890_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	GYTAGTTCCRATGATGTTAGCTG	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11890_Mstr_Wil2021_COI	DRWB-085	1	BE11890_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	GTAGTGGTAACTACTCATGCTTTA	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11890_Pvir_Dia2013_COI	DRWB-085	1	BE11890_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	CTTAGTGGCATTAATTCGDAATCC	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11890_Rhar_Sim2018_COI	DRWB-085	1	BE11890_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	CTGCTGCTATTGCTCATG	130.93012
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11892_Aamu_Bax2006_COI	DRWB-087	1	BE11892_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	GCACAACCGGGATCTTTACTTC	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11892_Pcan_Bot2011_IGS	DRWB-087	1	BE11892_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-087	IGS	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	CGTAATCCTCAGTACTGGCTA	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11892_Pper_Dia2013_COI	DRWB-087	1	BE11892_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	CTTAGTGGCATTAATTCGDAATCC	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11892_Sspa_Oph2007_COI	DRWB-087	1	BE11892_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	GAAGGCCTTCCGATGATC	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11892_Upin_Bot2011_COI	DRWB-087	1	BE11892_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	CTTTAATTACAGCGTTTTTATTGTTGT	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11892_Vgib_Wil2025_COI	DRWB-087	1	BE11892_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	CCCCTCTTTACAAGTTCTTTAGGGT	130.93215
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11893_Asen_Wil2025_COI	DRWB-088	1	BE11893_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	GTTGTTGTTACAACACATGCTCTTTTG	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11893_Cjap_Sim2018_COI	DRWB-088	1	BE11893_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11893_Esin_And2018_Cyb	DRWB-088	1	BE11893_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-088	Cytb	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	ACCCCTCCTCATATCCAACCA	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11893_Mgig_Bot2012_COI	DRWB-088	1	BE11893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	TCTTATTCGTTGGAGACTTTATAACCCT	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11893_Mjap_Gib2022_COI	DRWB-088	1	BE11893_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	AATTTAATTGTGACTAGGCATGGTCTG	130.92859
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11894_Cmae_Bot2011_COI	DRWB-089	1	BE11894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	ATGAACAGTCTATCCTCCTTTAG	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11894_Hsan_Wil2021_COI	DRWB-089	1	BE11894_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	CCTGGGCCGGTATAGTAGGT	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11894_Mros_Gib2022_COI	DRWB-089	1	BE11894_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11894_Msal_Bot2012_COI	DRWB-089	1	BE11894_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	GYTAGTTCCRATGATGTTAGCTG	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11894_Mstr_Wil2021_COI	DRWB-089	1	BE11894_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	GTAGTGGTAACTACTCATGCTTTA	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11894_Pvir_Dia2013_COI	DRWB-089	1	BE11894_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	CTTAGTGGCATTAATTCGDAATCC	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11894_Rhar_Sim2018_COI	DRWB-089	1	BE11894_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	CTGCTGCTATTGCTCATG	130.92963
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11896_Aamu_Bax2006_COI	DRWB-091	1	BE11896_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	GCACAACCGGGATCTTTACTTC	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11896_Pcan_Bot2011_IGS	DRWB-091	1	BE11896_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-091	IGS	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	CGTAATCCTCAGTACTGGCTA	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11896_Pper_Dia2013_COI	DRWB-091	1	BE11896_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	CTTAGTGGCATTAATTCGDAATCC	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11896_Sspa_Oph2007_COI	DRWB-091	1	BE11896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	GAAGGCCTTCCGATGATC	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11896_Upin_Bot2011_COI	DRWB-091	1	BE11896_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	CTTTAATTACAGCGTTTTTATTGTTGT	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11896_Vgib_Wil2025_COI	DRWB-091	1	BE11896_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	CCCCTCTTTACAAGTTCTTTAGGGT	130.93164
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11897_Asen_Wil2025_COI	DRWB-092	1	BE11897_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	GTTGTTGTTACAACACATGCTCTTTTG	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11897_Cjap_Sim2018_COI	DRWB-092	1	BE11897_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11897_Esin_And2018_Cyb	DRWB-092	1	BE11897_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-092	Cytb	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	ACCCCTCCTCATATCCAACCA	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11897_Mgig_Bot2012_COI	DRWB-092	1	BE11897_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	TCTTATTCGTTGGAGACTTTATAACCCT	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11897_Mjap_Gib2022_COI	DRWB-092	1	BE11897_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	AATTTAATTGTGACTAGGCATGGTCTG	130.9281
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11898_Cmae_Bot2011_COI	DRWB-093	1	BE11898_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	ATGAACAGTCTATCCTCCTTTAG	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11898_Hsan_Wil2021_COI	DRWB-093	1	BE11898_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	CCTGGGCCGGTATAGTAGGT	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11898_Mros_Gib2022_COI	DRWB-093	1	BE11898_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11898_Msal_Bot2012_COI	DRWB-093	1	BE11898_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	GYTAGTTCCRATGATGTTAGCTG	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11898_Mstr_Wil2021_COI	DRWB-093	1	BE11898_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	GTAGTGGTAACTACTCATGCTTTA	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11898_Pvir_Dia2013_COI	DRWB-093	1	BE11898_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	CTTAGTGGCATTAATTCGDAATCC	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11898_Rhar_Sim2018_COI	DRWB-093	1	BE11898_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	CTGCTGCTATTGCTCATG	130.92915
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11900_Aamu_Bax2006_COI	DRWB-095	1	BE11900_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	GCACAACCGGGATCTTTACTTC	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11900_Pcan_Bot2011_IGS	DRWB-095	1	BE11900_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-095	IGS	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	CGTAATCCTCAGTACTGGCTA	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11900_Pper_Dia2013_COI	DRWB-095	1	BE11900_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	CTTAGTGGCATTAATTCGDAATCC	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11900_Sspa_Oph2007_COI	DRWB-095	1	BE11900_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	GAAGGCCTTCCGATGATC	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11900_Upin_Bot2011_COI	DRWB-095	1	BE11900_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	CTTTAATTACAGCGTTTTTATTGTTGT	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11900_Vgib_Wil2025_COI	DRWB-095	1	BE11900_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	CCCCTCTTTACAAGTTCTTTAGGGT	130.93115
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11901_Asen_Wil2025_COI	DRWB-096	1	BE11901_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	GTTGTTGTTACAACACATGCTCTTTTG	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11901_Cjap_Sim2018_COI	DRWB-096	1	BE11901_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11901_Esin_And2018_Cyb	DRWB-096	1	BE11901_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-096	Cytb	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	ACCCCTCCTCATATCCAACCA	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11901_Mgig_Bot2012_COI	DRWB-096	1	BE11901_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	TCTTATTCGTTGGAGACTTTATAACCCT	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11901_Mjap_Gib2022_COI	DRWB-096	1	BE11901_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	AATTTAATTGTGACTAGGCATGGTCTG	130.92764
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11902_Cmae_Bot2011_COI	DRWB-097	1	BE11902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	ATGAACAGTCTATCCTCCTTTAG	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11902_Hsan_Wil2021_COI	DRWB-097	1	BE11902_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	CCTGGGCCGGTATAGTAGGT	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11902_Mros_Gib2022_COI	DRWB-097	1	BE11902_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11902_Msal_Bot2012_COI	DRWB-097	1	BE11902_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	GYTAGTTCCRATGATGTTAGCTG	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11902_Mstr_Wil2021_COI	DRWB-097	1	BE11902_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	GTAGTGGTAACTACTCATGCTTTA	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11902_Pvir_Dia2013_COI	DRWB-097	1	BE11902_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	CTTAGTGGCATTAATTCGDAATCC	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11902_Rhar_Sim2018_COI	DRWB-097	1	BE11902_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	CTGCTGCTATTGCTCATG	130.92861
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11904_Aamu_Bax2006_COI	DRWB-099	1	BE11904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	GCACAACCGGGATCTTTACTTC	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11904_Pcan_Bot2011_IGS	DRWB-099	1	BE11904_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-099	IGS	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	CGTAATCCTCAGTACTGGCTA	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11904_Pper_Dia2013_COI	DRWB-099	1	BE11904_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	CTTAGTGGCATTAATTCGDAATCC	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11904_Sspa_Oph2007_COI	DRWB-099	1	BE11904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	GAAGGCCTTCCGATGATC	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11904_Upin_Bot2011_COI	DRWB-099	1	BE11904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	CTTTAATTACAGCGTTTTTATTGTTGT	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11904_Vgib_Wil2025_COI	DRWB-099	1	BE11904_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	CCCCTCTTTACAAGTTCTTTAGGGT	130.93073
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11913_Cmae_Bot2011_COI	GLTB-006	1	BE11913_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	ATGAACAGTCTATCCTCCTTTAG	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11913_Hsan_Wil2021_COI	GLTB-006	1	BE11913_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	CCTGGGCCGGTATAGTAGGT	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11913_Mros_Gib2022_COI	GLTB-006	1	BE11913_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11913_Msal_Bot2012_COI	GLTB-006	1	BE11913_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	GYTAGTTCCRATGATGTTAGCTG	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11913_Mstr_Wil2021_COI	GLTB-006	1	BE11913_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	GTAGTGGTAACTACTCATGCTTTA	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11913_Pvir_Dia2013_COI	GLTB-006	1	BE11913_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	CTTAGTGGCATTAATTCGDAATCC	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11913_Rhar_Sim2018_COI	GLTB-006	1	BE11913_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	CTGCTGCTATTGCTCATG	151.32344
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11915_Aamu_Bax2006_COI	GLTB-012	1	BE11915_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	GCACAACCGGGATCTTTACTTC	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11915_Pcan_Bot2011_IGS	GLTB-012	1	BE11915_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-012	IGS	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	CGTAATCCTCAGTACTGGCTA	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11915_Pper_Dia2013_COI	GLTB-012	1	BE11915_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	CTTAGTGGCATTAATTCGDAATCC	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11915_Sspa_Oph2007_COI	GLTB-012	1	BE11915_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	GAAGGCCTTCCGATGATC	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11915_Upin_Bot2011_COI	GLTB-012	1	BE11915_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	CTTTAATTACAGCGTTTTTATTGTTGT	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11915_Vgib_Wil2025_COI	GLTB-012	1	BE11915_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	CCCCTCTTTACAAGTTCTTTAGGGT	151.32407
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11916_Asen_Wil2025_COI	GLTB-024	1	BE11916_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	GTTGTTGTTACAACACATGCTCTTTTG	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11916_Cjap_Sim2018_COI	GLTB-024	1	BE11916_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11916_Esin_And2018_Cyb	GLTB-024	1	BE11916_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-024	Cytb	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	ACCCCTCCTCATATCCAACCA	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11916_Mgig_Bot2012_COI	GLTB-024	1	BE11916_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	TCTTATTCGTTGGAGACTTTATAACCCT	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11916_Mjap_Gib2022_COI	GLTB-024	1	BE11916_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	AATTTAATTGTGACTAGGCATGGTCTG	151.31444
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11917_Cmae_Bot2011_COI	GLTB-030	1	BE11917_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	ATGAACAGTCTATCCTCCTTTAG	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11917_Hsan_Wil2021_COI	GLTB-030	1	BE11917_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	CCTGGGCCGGTATAGTAGGT	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11917_Mros_Gib2022_COI	GLTB-030	1	BE11917_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11917_Msal_Bot2012_COI	GLTB-030	1	BE11917_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	GYTAGTTCCRATGATGTTAGCTG	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11917_Mstr_Wil2021_COI	GLTB-030	1	BE11917_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	GTAGTGGTAACTACTCATGCTTTA	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11917_Pvir_Dia2013_COI	GLTB-030	1	BE11917_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	CTTAGTGGCATTAATTCGDAATCC	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11917_Rhar_Sim2018_COI	GLTB-030	1	BE11917_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	CTGCTGCTATTGCTCATG	151.31018
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11919_Aamu_Bax2006_COI	GLTB-005	1	BE11919_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	GCACAACCGGGATCTTTACTTC	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11919_Pcan_Bot2011_IGS	GLTB-005	1	BE11919_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-005	IGS	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	CGTAATCCTCAGTACTGGCTA	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11919_Pper_Dia2013_COI	GLTB-005	1	BE11919_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	CTTAGTGGCATTAATTCGDAATCC	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11919_Sspa_Oph2007_COI	GLTB-005	1	BE11919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	GAAGGCCTTCCGATGATC	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11919_Upin_Bot2011_COI	GLTB-005	1	BE11919_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	CTTTAATTACAGCGTTTTTATTGTTGT	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11919_Vgib_Wil2025_COI	GLTB-005	1	BE11919_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	CCCCTCTTTACAAGTTCTTTAGGGT	151.32604
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11920_Cmae_Bot2011_COI	GLTB-004	1	BE11920_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	ATGAACAGTCTATCCTCCTTTAG	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11920_Hsan_Wil2021_COI	GLTB-004	1	BE11920_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	CCTGGGCCGGTATAGTAGGT	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11920_Mros_Gib2022_COI	GLTB-004	1	BE11920_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11920_Msal_Bot2012_COI	GLTB-004	1	BE11920_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	GYTAGTTCCRATGATGTTAGCTG	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11920_Mstr_Wil2021_COI	GLTB-004	1	BE11920_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	GTAGTGGTAACTACTCATGCTTTA	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11920_Pvir_Dia2013_COI	GLTB-004	1	BE11920_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	CTTAGTGGCATTAATTCGDAATCC	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11920_Rhar_Sim2018_COI	GLTB-004	1	BE11920_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	CTGCTGCTATTGCTCATG	151.32434
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11922_Aamu_Bax2006_COI	GLTB-011	1	BE11922_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	GCACAACCGGGATCTTTACTTC	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11922_Pcan_Bot2011_IGS	GLTB-011	1	BE11922_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-011	IGS	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	CGTAATCCTCAGTACTGGCTA	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11922_Pper_Dia2013_COI	GLTB-011	1	BE11922_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	CTTAGTGGCATTAATTCGDAATCC	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11922_Sspa_Oph2007_COI	GLTB-011	1	BE11922_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	GAAGGCCTTCCGATGATC	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11922_Upin_Bot2011_COI	GLTB-011	1	BE11922_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	CTTTAATTACAGCGTTTTTATTGTTGT	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11922_Vgib_Wil2025_COI	GLTB-011	1	BE11922_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	CCCCTCTTTACAAGTTCTTTAGGGT	151.32207
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11923_Asen_Wil2025_COI	GLTB-017	1	BE11923_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	GTTGTTGTTACAACACATGCTCTTTTG	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11923_Cjap_Sim2018_COI	GLTB-017	1	BE11923_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11923_Esin_And2018_Cyb	GLTB-017	1	BE11923_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-017	Cytb	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	ACCCCTCCTCATATCCAACCA	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11923_Mgig_Bot2012_COI	GLTB-017	1	BE11923_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	TCTTATTCGTTGGAGACTTTATAACCCT	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11923_Mjap_Gib2022_COI	GLTB-017	1	BE11923_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	AATTTAATTGTGACTAGGCATGGTCTG	151.32109
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11924_Cmae_Bot2011_COI	GLTB-023	1	BE11924_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	ATGAACAGTCTATCCTCCTTTAG	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11924_Hsan_Wil2021_COI	GLTB-023	1	BE11924_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	CCTGGGCCGGTATAGTAGGT	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11924_Mros_Gib2022_COI	GLTB-023	1	BE11924_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11924_Msal_Bot2012_COI	GLTB-023	1	BE11924_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	GYTAGTTCCRATGATGTTAGCTG	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11924_Mstr_Wil2021_COI	GLTB-023	1	BE11924_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	GTAGTGGTAACTACTCATGCTTTA	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11924_Pvir_Dia2013_COI	GLTB-023	1	BE11924_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	CTTAGTGGCATTAATTCGDAATCC	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11924_Rhar_Sim2018_COI	GLTB-023	1	BE11924_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	CTGCTGCTATTGCTCATG	151.31305
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11926_Aamu_Bax2006_COI	GLTB-016	1	BE11926_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	GCACAACCGGGATCTTTACTTC	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11926_Pcan_Bot2011_IGS	GLTB-016	1	BE11926_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-016	IGS	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	CGTAATCCTCAGTACTGGCTA	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11926_Pper_Dia2013_COI	GLTB-016	1	BE11926_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	CTTAGTGGCATTAATTCGDAATCC	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11926_Sspa_Oph2007_COI	GLTB-016	1	BE11926_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	GAAGGCCTTCCGATGATC	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11926_Upin_Bot2011_COI	GLTB-016	1	BE11926_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	CTTTAATTACAGCGTTTTTATTGTTGT	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11926_Vgib_Wil2025_COI	GLTB-016	1	BE11926_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	CCCCTCTTTACAAGTTCTTTAGGGT	151.31957
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11927_Asen_Wil2025_COI	GLTB-003	1	BE11927_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	GTTGTTGTTACAACACATGCTCTTTTG	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11927_Cjap_Sim2018_COI	GLTB-003	1	BE11927_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11927_Esin_And2018_Cyb	GLTB-003	1	BE11927_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-003	Cytb	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	ACCCCTCCTCATATCCAACCA	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11927_Mgig_Bot2012_COI	GLTB-003	1	BE11927_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	TCTTATTCGTTGGAGACTTTATAACCCT	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11927_Mjap_Gib2022_COI	GLTB-003	1	BE11927_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	AATTTAATTGTGACTAGGCATGGTCTG	151.32561
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11928_Cmae_Bot2011_COI	GLTB-028	1	BE11928_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	ATGAACAGTCTATCCTCCTTTAG	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11928_Hsan_Wil2021_COI	GLTB-028	1	BE11928_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	CCTGGGCCGGTATAGTAGGT	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11928_Mros_Gib2022_COI	GLTB-028	1	BE11928_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11928_Msal_Bot2012_COI	GLTB-028	1	BE11928_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	GYTAGTTCCRATGATGTTAGCTG	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11928_Mstr_Wil2021_COI	GLTB-028	1	BE11928_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	GTAGTGGTAACTACTCATGCTTTA	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11928_Pvir_Dia2013_COI	GLTB-028	1	BE11928_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	CTTAGTGGCATTAATTCGDAATCC	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11928_Rhar_Sim2018_COI	GLTB-028	1	BE11928_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	CTGCTGCTATTGCTCATG	151.30658
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11930_Asen_Wil2025_COI	GLTB-015	1	BE11930_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	GTTGTTGTTACAACACATGCTCTTTTG	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11930_Cjap_Sim2018_COI	GLTB-015	1	BE11930_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11930_Esin_And2018_Cyb	GLTB-015	1	BE11930_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-015	Cytb	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	ACCCCTCCTCATATCCAACCA	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11930_Mgig_Bot2012_COI	GLTB-015	1	BE11930_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	TCTTATTCGTTGGAGACTTTATAACCCT	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11930_Mjap_Gib2022_COI	GLTB-015	1	BE11930_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	AATTTAATTGTGACTAGGCATGGTCTG	151.31768
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11931_Cmae_Bot2011_COI	GLTB-027	1	BE11931_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	ATGAACAGTCTATCCTCCTTTAG	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11931_Hsan_Wil2021_COI	GLTB-027	1	BE11931_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	CCTGGGCCGGTATAGTAGGT	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11931_Mros_Gib2022_COI	GLTB-027	1	BE11931_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11931_Msal_Bot2012_COI	GLTB-027	1	BE11931_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	GYTAGTTCCRATGATGTTAGCTG	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11931_Mstr_Wil2021_COI	GLTB-027	1	BE11931_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	GTAGTGGTAACTACTCATGCTTTA	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11931_Pvir_Dia2013_COI	GLTB-027	1	BE11931_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	CTTAGTGGCATTAATTCGDAATCC	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11931_Rhar_Sim2018_COI	GLTB-027	1	BE11931_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	CTGCTGCTATTGCTCATG	151.31963
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11933_Aamu_Bax2006_COI	GLTB-014	1	BE11933_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	GCACAACCGGGATCTTTACTTC	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11933_Pcan_Bot2011_IGS	GLTB-014	1	BE11933_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-014	IGS	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	CGTAATCCTCAGTACTGGCTA	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11933_Pper_Dia2013_COI	GLTB-014	1	BE11933_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	CTTAGTGGCATTAATTCGDAATCC	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11933_Sspa_Oph2007_COI	GLTB-014	1	BE11933_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	GAAGGCCTTCCGATGATC	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11933_Upin_Bot2011_COI	GLTB-014	1	BE11933_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	CTTTAATTACAGCGTTTTTATTGTTGT	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11933_Vgib_Wil2025_COI	GLTB-014	1	BE11933_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	CCCCTCTTTACAAGTTCTTTAGGGT	151.31603
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11934_Asen_Wil2025_COI	GLTB-008	1	BE11934_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	GTTGTTGTTACAACACATGCTCTTTTG	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11934_Cjap_Sim2018_COI	GLTB-008	1	BE11934_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11934_Esin_And2018_Cyb	GLTB-008	1	BE11934_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-008	Cytb	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	ACCCCTCCTCATATCCAACCA	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11934_Mgig_Bot2012_COI	GLTB-008	1	BE11934_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	TCTTATTCGTTGGAGACTTTATAACCCT	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11934_Mjap_Gib2022_COI	GLTB-008	1	BE11934_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	AATTTAATTGTGACTAGGCATGGTCTG	151.31702
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11935_Cmae_Bot2011_COI	GLTB-026	1	BE11935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	ATGAACAGTCTATCCTCCTTTAG	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11935_Hsan_Wil2021_COI	GLTB-026	1	BE11935_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	CCTGGGCCGGTATAGTAGGT	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11935_Mros_Gib2022_COI	GLTB-026	1	BE11935_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11935_Msal_Bot2012_COI	GLTB-026	1	BE11935_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	GYTAGTTCCRATGATGTTAGCTG	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11935_Mstr_Wil2021_COI	GLTB-026	1	BE11935_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	GTAGTGGTAACTACTCATGCTTTA	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11935_Pvir_Dia2013_COI	GLTB-026	1	BE11935_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	CTTAGTGGCATTAATTCGDAATCC	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11935_Rhar_Sim2018_COI	GLTB-026	1	BE11935_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	CTGCTGCTATTGCTCATG	151.31817
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11937_Aamu_Bax2006_COI	GLTB-019	1	BE11937_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	GCACAACCGGGATCTTTACTTC	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11937_Pcan_Bot2011_IGS	GLTB-019	1	BE11937_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-019	IGS	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	CGTAATCCTCAGTACTGGCTA	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11937_Pper_Dia2013_COI	GLTB-019	1	BE11937_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	CTTAGTGGCATTAATTCGDAATCC	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11937_Sspa_Oph2007_COI	GLTB-019	1	BE11937_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	GAAGGCCTTCCGATGATC	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11937_Upin_Bot2011_COI	GLTB-019	1	BE11937_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	CTTTAATTACAGCGTTTTTATTGTTGT	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11937_Vgib_Wil2025_COI	GLTB-019	1	BE11937_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	CCCCTCTTTACAAGTTCTTTAGGGT	151.30599
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11938_Asen_Wil2025_COI	GLTB-025	1	BE11938_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	GTTGTTGTTACAACACATGCTCTTTTG	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11938_Cjap_Sim2018_COI	GLTB-025	1	BE11938_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11938_Esin_And2018_Cyb	GLTB-025	1	BE11938_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-025	Cytb	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	ACCCCTCCTCATATCCAACCA	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11938_Mgig_Bot2012_COI	GLTB-025	1	BE11938_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	TCTTATTCGTTGGAGACTTTATAACCCT	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11938_Mjap_Gib2022_COI	GLTB-025	1	BE11938_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	AATTTAATTGTGACTAGGCATGGTCTG	151.31631
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11939_Cmae_Bot2011_COI	GLTB-007	1	BE11939_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	ATGAACAGTCTATCCTCCTTTAG	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11939_Hsan_Wil2021_COI	GLTB-007	1	BE11939_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	CCTGGGCCGGTATAGTAGGT	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11939_Mros_Gib2022_COI	GLTB-007	1	BE11939_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11939_Msal_Bot2012_COI	GLTB-007	1	BE11939_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	GYTAGTTCCRATGATGTTAGCTG	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11939_Mstr_Wil2021_COI	GLTB-007	1	BE11939_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	GTAGTGGTAACTACTCATGCTTTA	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11939_Pvir_Dia2013_COI	GLTB-007	1	BE11939_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	CTTAGTGGCATTAATTCGDAATCC	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11939_Rhar_Sim2018_COI	GLTB-007	1	BE11939_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	CTGCTGCTATTGCTCATG	151.32538
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11940_Aamu_Bax2006_COI	GLTB-013	1	BE11940_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	GCACAACCGGGATCTTTACTTC	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11940_Pcan_Bot2011_IGS	GLTB-013	1	BE11940_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-013	IGS	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	CGTAATCCTCAGTACTGGCTA	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11940_Pper_Dia2013_COI	GLTB-013	1	BE11940_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	CTTAGTGGCATTAATTCGDAATCC	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11940_Sspa_Oph2007_COI	GLTB-013	1	BE11940_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	GAAGGCCTTCCGATGATC	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11940_Upin_Bot2011_COI	GLTB-013	1	BE11940_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	CTTTAATTACAGCGTTTTTATTGTTGT	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11940_Vgib_Wil2025_COI	GLTB-013	1	BE11940_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	CCCCTCTTTACAAGTTCTTTAGGGT	151.30703
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11941_Asen_Wil2025_COI	GLTB-001	1	BE11941_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	GTTGTTGTTACAACACATGCTCTTTTG	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11941_Cjap_Sim2018_COI	GLTB-001	1	BE11941_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11941_Esin_And2018_Cyb	GLTB-001	1	BE11941_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-001	Cytb	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	ACCCCTCCTCATATCCAACCA	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11941_Mgig_Bot2012_COI	GLTB-001	1	BE11941_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	TCTTATTCGTTGGAGACTTTATAACCCT	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11941_Mjap_Gib2022_COI	GLTB-001	1	BE11941_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	AATTTAATTGTGACTAGGCATGGTCTG	151.32441
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11942_Cmae_Bot2011_COI	GLTB-002	1	BE11942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	ATGAACAGTCTATCCTCCTTTAG	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11942_Hsan_Wil2021_COI	GLTB-002	1	BE11942_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	CCTGGGCCGGTATAGTAGGT	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11942_Mros_Gib2022_COI	GLTB-002	1	BE11942_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11942_Msal_Bot2012_COI	GLTB-002	1	BE11942_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	GYTAGTTCCRATGATGTTAGCTG	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11942_Mstr_Wil2021_COI	GLTB-002	1	BE11942_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	GTAGTGGTAACTACTCATGCTTTA	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11942_Pvir_Dia2013_COI	GLTB-002	1	BE11942_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	CTTAGTGGCATTAATTCGDAATCC	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11942_Rhar_Sim2018_COI	GLTB-002	1	BE11942_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	CTGCTGCTATTGCTCATG	151.32348
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11944_Aamu_Bax2006_COI	GLTB-032	1	BE11944_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	GCACAACCGGGATCTTTACTTC	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11944_Pcan_Bot2011_IGS	GLTB-032	1	BE11944_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-032	IGS	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	CGTAATCCTCAGTACTGGCTA	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11944_Pper_Dia2013_COI	GLTB-032	1	BE11944_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	CTTAGTGGCATTAATTCGDAATCC	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11944_Sspa_Oph2007_COI	GLTB-032	1	BE11944_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	GAAGGCCTTCCGATGATC	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11944_Upin_Bot2011_COI	GLTB-032	1	BE11944_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	CTTTAATTACAGCGTTTTTATTGTTGT	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11944_Vgib_Wil2025_COI	GLTB-032	1	BE11944_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	CCCCTCTTTACAAGTTCTTTAGGGT	151.31378
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11945_Asen_Wil2025_COI	GLTB-033	1	BE11945_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	GTTGTTGTTACAACACATGCTCTTTTG	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11945_Cjap_Sim2018_COI	GLTB-033	1	BE11945_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11945_Esin_And2018_Cyb	GLTB-033	1	BE11945_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-033	Cytb	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	ACCCCTCCTCATATCCAACCA	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11945_Mgig_Bot2012_COI	GLTB-033	1	BE11945_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	TCTTATTCGTTGGAGACTTTATAACCCT	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11945_Mjap_Gib2022_COI	GLTB-033	1	BE11945_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	AATTTAATTGTGACTAGGCATGGTCTG	151.31518
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11946_Cmae_Bot2011_COI	GLTB-034	1	BE11946_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	ATGAACAGTCTATCCTCCTTTAG	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11946_Mros_Gib2022_COI	GLTB-034	1	BE11946_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11946_Msal_Bot2012_COI	GLTB-034	1	BE11946_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	GYTAGTTCCRATGATGTTAGCTG	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11946_Mstr_Wil2021_COI	GLTB-034	1	BE11946_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	GTAGTGGTAACTACTCATGCTTTA	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11946_Pvir_Dia2013_COI	GLTB-034	1	BE11946_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	CTTAGTGGCATTAATTCGDAATCC	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11946_Rhar_Sim2018_COI	GLTB-034	1	BE11946_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	CTGCTGCTATTGCTCATG	151.31117
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11952_Asen_Wil2025_COI	GLTB-041	1	BE11952_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	GTTGTTGTTACAACACATGCTCTTTTG	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11952_Cjap_Sim2018_COI	GLTB-041	1	BE11952_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11952_Esin_And2018_Cyb	GLTB-041	1	BE11952_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-041	Cytb	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	ACCCCTCCTCATATCCAACCA	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11952_Mgig_Bot2012_COI	GLTB-041	1	BE11952_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	TCTTATTCGTTGGAGACTTTATAACCCT	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11952_Mjap_Gib2022_COI	GLTB-041	1	BE11952_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	AATTTAATTGTGACTAGGCATGGTCTG	151.27678
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11953_Cmae_Bot2011_COI	GLTB-042	1	BE11953_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	ATGAACAGTCTATCCTCCTTTAG	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11953_Hsan_Wil2021_COI	GLTB-042	1	BE11953_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	CCTGGGCCGGTATAGTAGGT	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11953_Mros_Gib2022_COI	GLTB-042	1	BE11953_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11953_Msal_Bot2012_COI	GLTB-042	1	BE11953_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	GYTAGTTCCRATGATGTTAGCTG	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11953_Mstr_Wil2021_COI	GLTB-042	1	BE11953_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	GTAGTGGTAACTACTCATGCTTTA	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11953_Pvir_Dia2013_COI	GLTB-042	1	BE11953_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	CTTAGTGGCATTAATTCGDAATCC	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11953_Rhar_Sim2018_COI	GLTB-042	1	BE11953_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	CTGCTGCTATTGCTCATG	151.2668
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11955_Aamu_Bax2006_COI	GLTB-044	1	BE11955_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	GCACAACCGGGATCTTTACTTC	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11955_Pcan_Bot2011_IGS	GLTB-044	1	BE11955_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-044	IGS	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	CGTAATCCTCAGTACTGGCTA	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11955_Pper_Dia2013_COI	GLTB-044	1	BE11955_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	CTTAGTGGCATTAATTCGDAATCC	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11955_Sspa_Oph2007_COI	GLTB-044	1	BE11955_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	GAAGGCCTTCCGATGATC	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11955_Upin_Bot2011_COI	GLTB-044	1	BE11955_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	CTTTAATTACAGCGTTTTTATTGTTGT	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11955_Vgib_Wil2025_COI	GLTB-044	1	BE11955_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	CCCCTCTTTACAAGTTCTTTAGGGT	151.27042
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11956_Asen_Wil2025_COI	GLTB-045	1	BE11956_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	GTTGTTGTTACAACACATGCTCTTTTG	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11956_Cjap_Sim2018_COI	GLTB-045	1	BE11956_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11956_Esin_And2018_Cyb	GLTB-045	1	BE11956_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-045	Cytb	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	ACCCCTCCTCATATCCAACCA	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11956_Mgig_Bot2012_COI	GLTB-045	1	BE11956_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	TCTTATTCGTTGGAGACTTTATAACCCT	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11956_Mjap_Gib2022_COI	GLTB-045	1	BE11956_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	AATTTAATTGTGACTAGGCATGGTCTG	151.27238
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11957_Cmae_Bot2011_COI	GLTB-046	1	BE11957_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	ATGAACAGTCTATCCTCCTTTAG	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11957_Hsan_Wil2021_COI	GLTB-046	1	BE11957_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	CCTGGGCCGGTATAGTAGGT	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11957_Mros_Gib2022_COI	GLTB-046	1	BE11957_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11957_Msal_Bot2012_COI	GLTB-046	1	BE11957_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	GYTAGTTCCRATGATGTTAGCTG	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11957_Mstr_Wil2021_COI	GLTB-046	1	BE11957_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	GTAGTGGTAACTACTCATGCTTTA	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11957_Pvir_Dia2013_COI	GLTB-046	1	BE11957_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	CTTAGTGGCATTAATTCGDAATCC	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11957_Rhar_Sim2018_COI	GLTB-046	1	BE11957_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	CTGCTGCTATTGCTCATG	151.27382
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11959_Aamu_Bax2006_COI	GLTB-047	1	BE11959_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	GCACAACCGGGATCTTTACTTC	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11959_Pcan_Bot2011_IGS	GLTB-047	1	BE11959_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-047	IGS	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	CGTAATCCTCAGTACTGGCTA	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11959_Pper_Dia2013_COI	GLTB-047	1	BE11959_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	CTTAGTGGCATTAATTCGDAATCC	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11959_Sspa_Oph2007_COI	GLTB-047	1	BE11959_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	GAAGGCCTTCCGATGATC	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11959_Upin_Bot2011_COI	GLTB-047	1	BE11959_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	CTTTAATTACAGCGTTTTTATTGTTGT	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11959_Vgib_Wil2025_COI	GLTB-047	1	BE11959_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	CCCCTCTTTACAAGTTCTTTAGGGT	151.27558
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11960_Cmae_Bot2011_COI	GLTB-048	1	BE11960_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	ATGAACAGTCTATCCTCCTTTAG	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11960_Hsan_Wil2021_COI	GLTB-048	1	BE11960_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	CCTGGGCCGGTATAGTAGGT	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11960_Mros_Gib2022_COI	GLTB-048	1	BE11960_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11960_Msal_Bot2012_COI	GLTB-048	1	BE11960_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	GYTAGTTCCRATGATGTTAGCTG	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11960_Mstr_Wil2021_COI	GLTB-048	1	BE11960_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	GTAGTGGTAACTACTCATGCTTTA	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11960_Pvir_Dia2013_COI	GLTB-048	1	BE11960_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	CTTAGTGGCATTAATTCGDAATCC	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11960_Rhar_Sim2018_COI	GLTB-048	1	BE11960_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	CTGCTGCTATTGCTCATG	151.26536
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11962_Aamu_Bax2006_COI	GLTB-050	1	BE11962_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	GCACAACCGGGATCTTTACTTC	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11962_Pcan_Bot2011_IGS	GLTB-050	1	BE11962_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-050	IGS	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	CGTAATCCTCAGTACTGGCTA	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11962_Pper_Dia2013_COI	GLTB-050	1	BE11962_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	CTTAGTGGCATTAATTCGDAATCC	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11962_Sspa_Oph2007_COI	GLTB-050	1	BE11962_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	GAAGGCCTTCCGATGATC	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11962_Upin_Bot2011_COI	GLTB-050	1	BE11962_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	CTTTAATTACAGCGTTTTTATTGTTGT	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11962_Vgib_Wil2025_COI	GLTB-050	1	BE11962_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	CCCCTCTTTACAAGTTCTTTAGGGT	151.26908
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11963_Asen_Wil2025_COI	GLTB-051	1	BE11963_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	GTTGTTGTTACAACACATGCTCTTTTG	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11963_Cjap_Sim2018_COI	GLTB-051	1	BE11963_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11963_Esin_And2018_Cyb	GLTB-051	1	BE11963_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-051	Cytb	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	ACCCCTCCTCATATCCAACCA	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11963_Mgig_Bot2012_COI	GLTB-051	1	BE11963_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	TCTTATTCGTTGGAGACTTTATAACCCT	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11963_Mjap_Gib2022_COI	GLTB-051	1	BE11963_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	AATTTAATTGTGACTAGGCATGGTCTG	151.27092
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11964_Cmae_Bot2011_COI	GLTB-052	1	BE11964_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	ATGAACAGTCTATCCTCCTTTAG	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11964_Hsan_Wil2021_COI	GLTB-052	1	BE11964_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	CCTGGGCCGGTATAGTAGGT	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11964_Mros_Gib2022_COI	GLTB-052	1	BE11964_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11964_Msal_Bot2012_COI	GLTB-052	1	BE11964_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	GYTAGTTCCRATGATGTTAGCTG	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11964_Mstr_Wil2021_COI	GLTB-052	1	BE11964_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	GTAGTGGTAACTACTCATGCTTTA	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11964_Pvir_Dia2013_COI	GLTB-052	1	BE11964_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	CTTAGTGGCATTAATTCGDAATCC	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11964_Rhar_Sim2018_COI	GLTB-052	1	BE11964_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	CTGCTGCTATTGCTCATG	151.27254
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11966_Aamu_Bax2006_COI	GLTB-055	1	BE11966_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	GCACAACCGGGATCTTTACTTC	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11966_Pcan_Bot2011_IGS	GLTB-055	1	BE11966_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-055	IGS	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	CGTAATCCTCAGTACTGGCTA	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11966_Pper_Dia2013_COI	GLTB-055	1	BE11966_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	CTTAGTGGCATTAATTCGDAATCC	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11966_Sspa_Oph2007_COI	GLTB-055	1	BE11966_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	GAAGGCCTTCCGATGATC	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11966_Upin_Bot2011_COI	GLTB-055	1	BE11966_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	CTTTAATTACAGCGTTTTTATTGTTGT	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11966_Vgib_Wil2025_COI	GLTB-055	1	BE11966_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	CCCCTCTTTACAAGTTCTTTAGGGT	151.26628
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11967_Asen_Wil2025_COI	GLTB-056	1	BE11967_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	GTTGTTGTTACAACACATGCTCTTTTG	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11967_Cjap_Sim2018_COI	GLTB-056	1	BE11967_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11967_Esin_And2018_Cyb	GLTB-056	1	BE11967_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-056	Cytb	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	ACCCCTCCTCATATCCAACCA	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11967_Mgig_Bot2012_COI	GLTB-056	1	BE11967_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	TCTTATTCGTTGGAGACTTTATAACCCT	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11967_Mjap_Gib2022_COI	GLTB-056	1	BE11967_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	AATTTAATTGTGACTAGGCATGGTCTG	151.26783
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11968_Cmae_Bot2011_COI	GLTB-057	1	BE11968_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	ATGAACAGTCTATCCTCCTTTAG	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11968_Hsan_Wil2021_COI	GLTB-057	1	BE11968_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	CCTGGGCCGGTATAGTAGGT	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11968_Mros_Gib2022_COI	GLTB-057	1	BE11968_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11968_Msal_Bot2012_COI	GLTB-057	1	BE11968_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	GYTAGTTCCRATGATGTTAGCTG	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11968_Mstr_Wil2021_COI	GLTB-057	1	BE11968_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	GTAGTGGTAACTACTCATGCTTTA	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11968_Pvir_Dia2013_COI	GLTB-057	1	BE11968_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	CTTAGTGGCATTAATTCGDAATCC	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11968_Rhar_Sim2018_COI	GLTB-057	1	BE11968_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	CTGCTGCTATTGCTCATG	151.25752
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11970_Asen_Wil2025_COI	GLTB-059	1	BE11970_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	GTTGTTGTTACAACACATGCTCTTTTG	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11970_Cjap_Sim2018_COI	GLTB-059	1	BE11970_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11970_Esin_And2018_Cyb	GLTB-059	1	BE11970_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-059	Cytb	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	ACCCCTCCTCATATCCAACCA	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11970_Mgig_Bot2012_COI	GLTB-059	1	BE11970_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	TCTTATTCGTTGGAGACTTTATAACCCT	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11970_Mjap_Gib2022_COI	GLTB-059	1	BE11970_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	AATTTAATTGTGACTAGGCATGGTCTG	151.26152
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11971_Cmae_Bot2011_COI	GLTB-060	1	BE11971_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	ATGAACAGTCTATCCTCCTTTAG	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11971_Hsan_Wil2021_COI	GLTB-060	1	BE11971_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	CCTGGGCCGGTATAGTAGGT	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11971_Mros_Gib2022_COI	GLTB-060	1	BE11971_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11971_Msal_Bot2012_COI	GLTB-060	1	BE11971_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	GYTAGTTCCRATGATGTTAGCTG	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11971_Mstr_Wil2021_COI	GLTB-060	1	BE11971_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	GTAGTGGTAACTACTCATGCTTTA	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11971_Pvir_Dia2013_COI	GLTB-060	1	BE11971_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	CTTAGTGGCATTAATTCGDAATCC	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11971_Rhar_Sim2018_COI	GLTB-060	1	BE11971_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	CTGCTGCTATTGCTCATG	151.26334
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11973_Aamu_Bax2006_COI	GLTB-062	1	BE11973_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	GCACAACCGGGATCTTTACTTC	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11973_Pcan_Bot2011_IGS	GLTB-062	1	BE11973_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-062	IGS	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	CGTAATCCTCAGTACTGGCTA	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11973_Pper_Dia2013_COI	GLTB-062	1	BE11973_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	CTTAGTGGCATTAATTCGDAATCC	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11973_Sspa_Oph2007_COI	GLTB-062	1	BE11973_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	GAAGGCCTTCCGATGATC	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11973_Upin_Bot2011_COI	GLTB-062	1	BE11973_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	CTTTAATTACAGCGTTTTTATTGTTGT	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11973_Vgib_Wil2025_COI	GLTB-062	1	BE11973_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	CCCCTCTTTACAAGTTCTTTAGGGT	151.25297
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11974_Asen_Wil2025_COI	GLTB-063	1	BE11974_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	GTTGTTGTTACAACACATGCTCTTTTG	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11974_Cjap_Sim2018_COI	GLTB-063	1	BE11974_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11974_Esin_And2018_Cyb	GLTB-063	1	BE11974_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-063	Cytb	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	ACCCCTCCTCATATCCAACCA	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11974_Mgig_Bot2012_COI	GLTB-063	1	BE11974_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	TCTTATTCGTTGGAGACTTTATAACCCT	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11974_Mjap_Gib2022_COI	GLTB-063	1	BE11974_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	AATTTAATTGTGACTAGGCATGGTCTG	151.25482
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11975_Cmae_Bot2011_COI	GLTB-064	1	BE11975_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	ATGAACAGTCTATCCTCCTTTAG	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11975_Hsan_Wil2021_COI	GLTB-064	1	BE11975_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	CCTGGGCCGGTATAGTAGGT	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11975_Mros_Gib2022_COI	GLTB-064	1	BE11975_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11975_Msal_Bot2012_COI	GLTB-064	1	BE11975_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	GYTAGTTCCRATGATGTTAGCTG	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11975_Mstr_Wil2021_COI	GLTB-064	1	BE11975_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	GTAGTGGTAACTACTCATGCTTTA	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11975_Pvir_Dia2013_COI	GLTB-064	1	BE11975_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	CTTAGTGGCATTAATTCGDAATCC	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11975_Rhar_Sim2018_COI	GLTB-064	1	BE11975_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	CTGCTGCTATTGCTCATG	151.25657
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11977_Aamu_Bax2006_COI	GLTB-066	1	BE11977_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	GCACAACCGGGATCTTTACTTC	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11977_Pcan_Bot2011_IGS	GLTB-066	1	BE11977_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-066	IGS	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	CGTAATCCTCAGTACTGGCTA	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11977_Pper_Dia2013_COI	GLTB-066	1	BE11977_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	CTTAGTGGCATTAATTCGDAATCC	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11977_Sspa_Oph2007_COI	GLTB-066	1	BE11977_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	GAAGGCCTTCCGATGATC	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11977_Upin_Bot2011_COI	GLTB-066	1	BE11977_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	CTTTAATTACAGCGTTTTTATTGTTGT	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11977_Vgib_Wil2025_COI	GLTB-066	1	BE11977_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	CCCCTCTTTACAAGTTCTTTAGGGT	151.2599
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11978_Asen_Wil2025_COI	GLTB-067	1	BE11978_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	GTTGTTGTTACAACACATGCTCTTTTG	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11978_Cjap_Sim2018_COI	GLTB-067	1	BE11978_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11978_Esin_And2018_Cyb	GLTB-067	1	BE11978_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-067	Cytb	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	ACCCCTCCTCATATCCAACCA	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11978_Mgig_Bot2012_COI	GLTB-067	1	BE11978_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	TCTTATTCGTTGGAGACTTTATAACCCT	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11978_Mjap_Gib2022_COI	GLTB-067	1	BE11978_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	AATTTAATTGTGACTAGGCATGGTCTG	151.26178
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11979_Cmae_Bot2011_COI	GLTB-068	1	BE11979_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	ATGAACAGTCTATCCTCCTTTAG	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11979_Hsan_Wil2021_COI	GLTB-068	1	BE11979_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	CCTGGGCCGGTATAGTAGGT	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11979_Mros_Gib2022_COI	GLTB-068	1	BE11979_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11979_Msal_Bot2012_COI	GLTB-068	1	BE11979_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	GYTAGTTCCRATGATGTTAGCTG	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11979_Mstr_Wil2021_COI	GLTB-068	1	BE11979_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	GTAGTGGTAACTACTCATGCTTTA	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11979_Pvir_Dia2013_COI	GLTB-068	1	BE11979_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	CTTAGTGGCATTAATTCGDAATCC	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11979_Rhar_Sim2018_COI	GLTB-068	1	BE11979_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	CTGCTGCTATTGCTCATG	151.25338
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11980_Aamu_Bax2006_COI	GLTB-069	1	BE11980_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	GCACAACCGGGATCTTTACTTC	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11980_Pcan_Bot2011_IGS	GLTB-069	1	BE11980_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-069	IGS	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	CGTAATCCTCAGTACTGGCTA	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11980_Pper_Dia2013_COI	GLTB-069	1	BE11980_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	CTTAGTGGCATTAATTCGDAATCC	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11980_Sspa_Oph2007_COI	GLTB-069	1	BE11980_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	GAAGGCCTTCCGATGATC	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11980_Upin_Bot2011_COI	GLTB-069	1	BE11980_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	CTTTAATTACAGCGTTTTTATTGTTGT	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11980_Vgib_Wil2025_COI	GLTB-069	1	BE11980_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	CCCCTCTTTACAAGTTCTTTAGGGT	151.25566
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11981_Asen_Wil2025_COI	GLTB-070	1	BE11981_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	GTTGTTGTTACAACACATGCTCTTTTG	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11981_Cjap_Sim2018_COI	GLTB-070	1	BE11981_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11981_Esin_And2018_Cyb	GLTB-070	1	BE11981_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-070	Cytb	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	ACCCCTCCTCATATCCAACCA	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11981_Mgig_Bot2012_COI	GLTB-070	1	BE11981_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	TCTTATTCGTTGGAGACTTTATAACCCT	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11981_Mjap_Gib2022_COI	GLTB-070	1	BE11981_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	AATTTAATTGTGACTAGGCATGGTCTG	151.2573
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11982_Cmae_Bot2011_COI	GLTB-036	1	BE11982_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	ATGAACAGTCTATCCTCCTTTAG	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11982_Hsan_Wil2021_COI	GLTB-036	1	BE11982_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	CCTGGGCCGGTATAGTAGGT	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11982_Mros_Gib2022_COI	GLTB-036	1	BE11982_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11982_Msal_Bot2012_COI	GLTB-036	1	BE11982_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	GYTAGTTCCRATGATGTTAGCTG	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11982_Mstr_Wil2021_COI	GLTB-036	1	BE11982_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	GTAGTGGTAACTACTCATGCTTTA	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11982_Pvir_Dia2013_COI	GLTB-036	1	BE11982_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	CTTAGTGGCATTAATTCGDAATCC	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11982_Rhar_Sim2018_COI	GLTB-036	1	BE11982_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	CTGCTGCTATTGCTCATG	151.27472
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11984_Aamu_Bax2006_COI	GLTB-038	1	BE11984_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	GCACAACCGGGATCTTTACTTC	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11984_Pcan_Bot2011_IGS	GLTB-038	1	BE11984_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-038	IGS	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	CGTAATCCTCAGTACTGGCTA	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11984_Pper_Dia2013_COI	GLTB-038	1	BE11984_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	CTTAGTGGCATTAATTCGDAATCC	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11984_Sspa_Oph2007_COI	GLTB-038	1	BE11984_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	GAAGGCCTTCCGATGATC	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11984_Upin_Bot2011_COI	GLTB-038	1	BE11984_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	CTTTAATTACAGCGTTTTTATTGTTGT	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11984_Vgib_Wil2025_COI	GLTB-038	1	BE11984_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	CCCCTCTTTACAAGTTCTTTAGGGT	151.27761
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11985_Asen_Wil2025_COI	GLTB-039	1	BE11985_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	GTTGTTGTTACAACACATGCTCTTTTG	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11985_Cjap_Sim2018_COI	GLTB-039	1	BE11985_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11985_Esin_And2018_Cyb	GLTB-039	1	BE11985_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-039	Cytb	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	ACCCCTCCTCATATCCAACCA	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11985_Mgig_Bot2012_COI	GLTB-039	1	BE11985_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	TCTTATTCGTTGGAGACTTTATAACCCT	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11985_Mjap_Gib2022_COI	GLTB-039	1	BE11985_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	AATTTAATTGTGACTAGGCATGGTCTG	151.27637
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11986_Cmae_Bot2011_COI	GLTB-040	1	BE11986_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	ATGAACAGTCTATCCTCCTTTAG	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11986_Hsan_Wil2021_COI	GLTB-040	1	BE11986_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	CCTGGGCCGGTATAGTAGGT	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11986_Mros_Gib2022_COI	GLTB-040	1	BE11986_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11986_Msal_Bot2012_COI	GLTB-040	1	BE11986_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	GYTAGTTCCRATGATGTTAGCTG	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11986_Mstr_Wil2021_COI	GLTB-040	1	BE11986_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	GTAGTGGTAACTACTCATGCTTTA	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11986_Pvir_Dia2013_COI	GLTB-040	1	BE11986_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	CTTAGTGGCATTAATTCGDAATCC	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11986_Rhar_Sim2018_COI	GLTB-040	1	BE11986_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	CTGCTGCTATTGCTCATG	151.27501
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11991_Aamu_Bax2006_COI	GLTB-071	1	BE11991_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	GCACAACCGGGATCTTTACTTC	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11991_Pcan_Bot2011_IGS	GLTB-071	1	BE11991_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-071	IGS	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	CGTAATCCTCAGTACTGGCTA	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11991_Pper_Dia2013_COI	GLTB-071	1	BE11991_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	CTTAGTGGCATTAATTCGDAATCC	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11991_Sspa_Oph2007_COI	GLTB-071	1	BE11991_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	GAAGGCCTTCCGATGATC	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11991_Upin_Bot2011_COI	GLTB-071	1	BE11991_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	CTTTAATTACAGCGTTTTTATTGTTGT	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11991_Vgib_Wil2025_COI	GLTB-071	1	BE11991_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	CCCCTCTTTACAAGTTCTTTAGGGT	151.18361
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11992_Asen_Wil2025_COI	GLTB-072	1	BE11992_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	GTTGTTGTTACAACACATGCTCTTTTG	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11992_Cjap_Sim2018_COI	GLTB-072	1	BE11992_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11992_Esin_And2018_Cyb	GLTB-072	1	BE11992_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-072	Cytb	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	ACCCCTCCTCATATCCAACCA	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11992_Mgig_Bot2012_COI	GLTB-072	1	BE11992_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	TCTTATTCGTTGGAGACTTTATAACCCT	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11992_Mjap_Gib2022_COI	GLTB-072	1	BE11992_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	AATTTAATTGTGACTAGGCATGGTCTG	151.1841
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11993_Cmae_Bot2011_COI	GLTB-073	1	BE11993_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	ATGAACAGTCTATCCTCCTTTAG	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11993_Hsan_Wil2021_COI	GLTB-073	1	BE11993_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	CCTGGGCCGGTATAGTAGGT	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11993_Mros_Gib2022_COI	GLTB-073	1	BE11993_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11993_Msal_Bot2012_COI	GLTB-073	1	BE11993_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	GYTAGTTCCRATGATGTTAGCTG	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11993_Mstr_Wil2021_COI	GLTB-073	1	BE11993_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	GTAGTGGTAACTACTCATGCTTTA	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11993_Pvir_Dia2013_COI	GLTB-073	1	BE11993_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	CTTAGTGGCATTAATTCGDAATCC	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11993_Rhar_Sim2018_COI	GLTB-073	1	BE11993_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	CTGCTGCTATTGCTCATG	151.18557
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11995_Aamu_Bax2006_COI	GLTB-075	1	BE11995_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	GCACAACCGGGATCTTTACTTC	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11995_Pcan_Bot2011_IGS	GLTB-075	1	BE11995_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-075	IGS	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	CGTAATCCTCAGTACTGGCTA	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11995_Pper_Dia2013_COI	GLTB-075	1	BE11995_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	CTTAGTGGCATTAATTCGDAATCC	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11995_Sspa_Oph2007_COI	GLTB-075	1	BE11995_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	GAAGGCCTTCCGATGATC	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11995_Upin_Bot2011_COI	GLTB-075	1	BE11995_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	CTTTAATTACAGCGTTTTTATTGTTGT	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11995_Vgib_Wil2025_COI	GLTB-075	1	BE11995_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	CCCCTCTTTACAAGTTCTTTAGGGT	151.18169
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11996_Asen_Wil2025_COI	GLTB-076	1	BE11996_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	GTTGTTGTTACAACACATGCTCTTTTG	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11996_Cjap_Sim2018_COI	GLTB-076	1	BE11996_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11996_Esin_And2018_Cyb	GLTB-076	1	BE11996_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-076	Cytb	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	ACCCCTCCTCATATCCAACCA	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11996_Mgig_Bot2012_COI	GLTB-076	1	BE11996_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	TCTTATTCGTTGGAGACTTTATAACCCT	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11996_Mjap_Gib2022_COI	GLTB-076	1	BE11996_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	AATTTAATTGTGACTAGGCATGGTCTG	151.18546
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11997_Cmae_Bot2011_COI	GLTB-077	1	BE11997_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	ATGAACAGTCTATCCTCCTTTAG	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11997_Hsan_Wil2021_COI	GLTB-077	1	BE11997_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	CCTGGGCCGGTATAGTAGGT	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11997_Mros_Gib2022_COI	GLTB-077	1	BE11997_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11997_Msal_Bot2012_COI	GLTB-077	1	BE11997_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	GYTAGTTCCRATGATGTTAGCTG	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11997_Mstr_Wil2021_COI	GLTB-077	1	BE11997_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	GTAGTGGTAACTACTCATGCTTTA	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11997_Pvir_Dia2013_COI	GLTB-077	1	BE11997_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	CTTAGTGGCATTAATTCGDAATCC	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11997_Rhar_Sim2018_COI	GLTB-077	1	BE11997_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	CTGCTGCTATTGCTCATG	151.18751
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11999_Aamu_Bax2006_COI	GLTB-079	1	BE11999_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	GCACAACCGGGATCTTTACTTC	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11999_Pcan_Bot2011_IGS	GLTB-079	1	BE11999_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-079	IGS	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	CGTAATCCTCAGTACTGGCTA	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11999_Pper_Dia2013_COI	GLTB-079	1	BE11999_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	CTTAGTGGCATTAATTCGDAATCC	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11999_Sspa_Oph2007_COI	GLTB-079	1	BE11999_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	GAAGGCCTTCCGATGATC	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11999_Upin_Bot2011_COI	GLTB-079	1	BE11999_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	CTTTAATTACAGCGTTTTTATTGTTGT	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11999_Vgib_Wil2025_COI	GLTB-079	1	BE11999_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	CCCCTCTTTACAAGTTCTTTAGGGT	151.18068
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12000_Cmae_Bot2011_COI	GLTB-080	1	BE12000_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	ATGAACAGTCTATCCTCCTTTAG	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12000_Hsan_Wil2021_COI	GLTB-080	1	BE12000_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	CCTGGGCCGGTATAGTAGGT	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12000_Mros_Gib2022_COI	GLTB-080	1	BE12000_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12000_Msal_Bot2012_COI	GLTB-080	1	BE12000_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	GYTAGTTCCRATGATGTTAGCTG	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12000_Mstr_Wil2021_COI	GLTB-080	1	BE12000_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	GTAGTGGTAACTACTCATGCTTTA	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12000_Pvir_Dia2013_COI	GLTB-080	1	BE12000_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	CTTAGTGGCATTAATTCGDAATCC	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12000_Rhar_Sim2018_COI	GLTB-080	1	BE12000_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	CTGCTGCTATTGCTCATG	151.18576
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12002_Aamu_Bax2006_COI	GLTB-082	1	BE12002_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	GCACAACCGGGATCTTTACTTC	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12002_Pcan_Bot2011_IGS	GLTB-082	1	BE12002_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-082	IGS	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	CGTAATCCTCAGTACTGGCTA	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12002_Pper_Dia2013_COI	GLTB-082	1	BE12002_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	CTTAGTGGCATTAATTCGDAATCC	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12002_Sspa_Oph2007_COI	GLTB-082	1	BE12002_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	GAAGGCCTTCCGATGATC	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12002_Upin_Bot2011_COI	GLTB-082	1	BE12002_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	CTTTAATTACAGCGTTTTTATTGTTGT	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12002_Vgib_Wil2025_COI	GLTB-082	1	BE12002_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	CCCCTCTTTACAAGTTCTTTAGGGT	151.18299
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12003_Asen_Wil2025_COI	GLTB-083	1	BE12003_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	GTTGTTGTTACAACACATGCTCTTTTG	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12003_Cjap_Sim2018_COI	GLTB-083	1	BE12003_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12003_Esin_And2018_Cyb	GLTB-083	1	BE12003_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-083	Cytb	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	ACCCCTCCTCATATCCAACCA	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12003_Mgig_Bot2012_COI	GLTB-083	1	BE12003_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	TCTTATTCGTTGGAGACTTTATAACCCT	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12003_Mjap_Gib2022_COI	GLTB-083	1	BE12003_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	AATTTAATTGTGACTAGGCATGGTCTG	151.18459
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12004_Cmae_Bot2011_COI	GLTB-084	1	BE12004_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	ATGAACAGTCTATCCTCCTTTAG	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12004_Hsan_Wil2021_COI	GLTB-084	1	BE12004_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	CCTGGGCCGGTATAGTAGGT	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12004_Mros_Gib2022_COI	GLTB-084	1	BE12004_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12004_Msal_Bot2012_COI	GLTB-084	1	BE12004_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	GYTAGTTCCRATGATGTTAGCTG	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12004_Mstr_Wil2021_COI	GLTB-084	1	BE12004_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	GTAGTGGTAACTACTCATGCTTTA	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12004_Pvir_Dia2013_COI	GLTB-084	1	BE12004_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	CTTAGTGGCATTAATTCGDAATCC	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12004_Rhar_Sim2018_COI	GLTB-084	1	BE12004_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	CTGCTGCTATTGCTCATG	151.18668
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12006_Aamu_Bax2006_COI	GLTB-086	1	BE12006_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	GCACAACCGGGATCTTTACTTC	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12006_Pcan_Bot2011_IGS	GLTB-086	1	BE12006_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-086	IGS	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	CGTAATCCTCAGTACTGGCTA	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12006_Pper_Dia2013_COI	GLTB-086	1	BE12006_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	CTTAGTGGCATTAATTCGDAATCC	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12006_Sspa_Oph2007_COI	GLTB-086	1	BE12006_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	GAAGGCCTTCCGATGATC	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12006_Upin_Bot2011_COI	GLTB-086	1	BE12006_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	CTTTAATTACAGCGTTTTTATTGTTGT	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12006_Vgib_Wil2025_COI	GLTB-086	1	BE12006_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	CCCCTCTTTACAAGTTCTTTAGGGT	151.18354
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12007_Asen_Wil2025_COI	GLTB-087	1	BE12007_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	GTTGTTGTTACAACACATGCTCTTTTG	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12007_Cjap_Sim2018_COI	GLTB-087	1	BE12007_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12007_Esin_And2018_Cyb	GLTB-087	1	BE12007_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-087	Cytb	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	ACCCCTCCTCATATCCAACCA	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12007_Mgig_Bot2012_COI	GLTB-087	1	BE12007_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	TCTTATTCGTTGGAGACTTTATAACCCT	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12007_Mjap_Gib2022_COI	GLTB-087	1	BE12007_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	AATTTAATTGTGACTAGGCATGGTCTG	151.18521
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12008_Cmae_Bot2011_COI	GLTB-088	1	BE12008_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	ATGAACAGTCTATCCTCCTTTAG	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12008_Hsan_Wil2021_COI	GLTB-088	1	BE12008_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	CCTGGGCCGGTATAGTAGGT	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12008_Mros_Gib2022_COI	GLTB-088	1	BE12008_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12008_Msal_Bot2012_COI	GLTB-088	1	BE12008_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	GYTAGTTCCRATGATGTTAGCTG	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12008_Mstr_Wil2021_COI	GLTB-088	1	BE12008_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	GTAGTGGTAACTACTCATGCTTTA	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12008_Pvir_Dia2013_COI	GLTB-088	1	BE12008_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	CTTAGTGGCATTAATTCGDAATCC	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12008_Rhar_Sim2018_COI	GLTB-088	1	BE12008_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	CTGCTGCTATTGCTCATG	151.18112
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12010_Asen_Wil2025_COI	GLTB-090	1	BE12010_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	GTTGTTGTTACAACACATGCTCTTTTG	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12010_Cjap_Sim2018_COI	GLTB-090	1	BE12010_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12010_Esin_And2018_Cyb	GLTB-090	1	BE12010_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-090	Cytb	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	ACCCCTCCTCATATCCAACCA	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12010_Mgig_Bot2012_COI	GLTB-090	1	BE12010_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	TCTTATTCGTTGGAGACTTTATAACCCT	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12010_Mjap_Gib2022_COI	GLTB-090	1	BE12010_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	AATTTAATTGTGACTAGGCATGGTCTG	151.1843
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12011_Cmae_Bot2011_COI	GLTB-091	1	BE12011_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	ATGAACAGTCTATCCTCCTTTAG	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12011_Hsan_Wil2021_COI	GLTB-091	1	BE12011_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	CCTGGGCCGGTATAGTAGGT	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12011_Mros_Gib2022_COI	GLTB-091	1	BE12011_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12011_Msal_Bot2012_COI	GLTB-091	1	BE12011_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	GYTAGTTCCRATGATGTTAGCTG	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12011_Mstr_Wil2021_COI	GLTB-091	1	BE12011_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	GTAGTGGTAACTACTCATGCTTTA	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12011_Pvir_Dia2013_COI	GLTB-091	1	BE12011_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	CTTAGTGGCATTAATTCGDAATCC	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12011_Rhar_Sim2018_COI	GLTB-091	1	BE12011_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	CTGCTGCTATTGCTCATG	151.17847
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12013_Aamu_Bax2006_COI	GLTB-093	1	BE12013_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	GCACAACCGGGATCTTTACTTC	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12013_Pcan_Bot2011_IGS	GLTB-093	1	BE12013_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-093	IGS	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	CGTAATCCTCAGTACTGGCTA	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12013_Pper_Dia2013_COI	GLTB-093	1	BE12013_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	CTTAGTGGCATTAATTCGDAATCC	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12013_Sspa_Oph2007_COI	GLTB-093	1	BE12013_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	GAAGGCCTTCCGATGATC	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12013_Upin_Bot2011_COI	GLTB-093	1	BE12013_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	CTTTAATTACAGCGTTTTTATTGTTGT	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12013_Vgib_Wil2025_COI	GLTB-093	1	BE12013_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	CCCCTCTTTACAAGTTCTTTAGGGT	151.18144
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12014_Asen_Wil2025_COI	GLTB-094	1	BE12014_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	GTTGTTGTTACAACACATGCTCTTTTG	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12014_Cjap_Sim2018_COI	GLTB-094	1	BE12014_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12014_Esin_And2018_Cyb	GLTB-094	1	BE12014_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-094	Cytb	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	ACCCCTCCTCATATCCAACCA	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12014_Mgig_Bot2012_COI	GLTB-094	1	BE12014_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	TCTTATTCGTTGGAGACTTTATAACCCT	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12014_Mjap_Gib2022_COI	GLTB-094	1	BE12014_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	AATTTAATTGTGACTAGGCATGGTCTG	151.17722
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12015_Cmae_Bot2011_COI	GLTB-095	1	BE12015_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	ATGAACAGTCTATCCTCCTTTAG	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12015_Hsan_Wil2021_COI	GLTB-095	1	BE12015_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	CCTGGGCCGGTATAGTAGGT	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12015_Mros_Gib2022_COI	GLTB-095	1	BE12015_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12015_Msal_Bot2012_COI	GLTB-095	1	BE12015_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	GYTAGTTCCRATGATGTTAGCTG	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12015_Mstr_Wil2021_COI	GLTB-095	1	BE12015_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	GTAGTGGTAACTACTCATGCTTTA	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12015_Pvir_Dia2013_COI	GLTB-095	1	BE12015_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	CTTAGTGGCATTAATTCGDAATCC	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12015_Rhar_Sim2018_COI	GLTB-095	1	BE12015_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	CTGCTGCTATTGCTCATG	151.17895
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12017_Aamu_Bax2006_COI	GLTB-097	1	BE12017_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	GCACAACCGGGATCTTTACTTC	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12017_Pcan_Bot2011_IGS	GLTB-097	1	BE12017_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-097	IGS	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	CGTAATCCTCAGTACTGGCTA	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12017_Pper_Dia2013_COI	GLTB-097	1	BE12017_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	CTTAGTGGCATTAATTCGDAATCC	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12017_Sspa_Oph2007_COI	GLTB-097	1	BE12017_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	GAAGGCCTTCCGATGATC	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12017_Upin_Bot2011_COI	GLTB-097	1	BE12017_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	CTTTAATTACAGCGTTTTTATTGTTGT	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12017_Vgib_Wil2025_COI	GLTB-097	1	BE12017_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	CCCCTCTTTACAAGTTCTTTAGGGT	151.17635
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12018_Asen_Wil2025_COI	GLTB-098	1	BE12018_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	GTTGTTGTTACAACACATGCTCTTTTG	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12018_Cjap_Sim2018_COI	GLTB-098	1	BE12018_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12018_Esin_And2018_Cyb	GLTB-098	1	BE12018_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-098	Cytb	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	ACCCCTCCTCATATCCAACCA	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12018_Mgig_Bot2012_COI	GLTB-098	1	BE12018_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	TCTTATTCGTTGGAGACTTTATAACCCT	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12018_Mjap_Gib2022_COI	GLTB-098	1	BE12018_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	AATTTAATTGTGACTAGGCATGGTCTG	151.17811
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12019_Cmae_Bot2011_COI	GLTB-099	1	BE12019_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	ATGAACAGTCTATCCTCCTTTAG	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12019_Hsan_Wil2021_COI	GLTB-099	1	BE12019_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	CCTGGGCCGGTATAGTAGGT	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12019_Mros_Gib2022_COI	GLTB-099	1	BE12019_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12019_Msal_Bot2012_COI	GLTB-099	1	BE12019_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	GYTAGTTCCRATGATGTTAGCTG	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12019_Mstr_Wil2021_COI	GLTB-099	1	BE12019_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	GTAGTGGTAACTACTCATGCTTTA	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12019_Pvir_Dia2013_COI	GLTB-099	1	BE12019_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	CTTAGTGGCATTAATTCGDAATCC	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12019_Rhar_Sim2018_COI	GLTB-099	1	BE12019_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	CTGCTGCTATTGCTCATG	151.17342
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12020_Aamu_Bax2006_COI	GLTB-100	1	BE12020_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	GCACAACCGGGATCTTTACTTC	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12020_Pcan_Bot2011_IGS	GLTB-100	1	BE12020_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-100	IGS	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	CGTAATCCTCAGTACTGGCTA	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12020_Pper_Dia2013_COI	GLTB-100	1	BE12020_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	CTTAGTGGCATTAATTCGDAATCC	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12020_Sspa_Oph2007_COI	GLTB-100	1	BE12020_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	GAAGGCCTTCCGATGATC	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12020_Upin_Bot2011_COI	GLTB-100	1	BE12020_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	CTTTAATTACAGCGTTTTTATTGTTGT	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12020_Vgib_Wil2025_COI	GLTB-100	1	BE12020_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	CCCCTCTTTACAAGTTCTTTAGGGT	151.17533
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12021_Asen_Wil2025_COI	GLTB-101	1	BE12021_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	GTTGTTGTTACAACACATGCTCTTTTG	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12021_Cjap_Sim2018_COI	GLTB-101	1	BE12021_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12021_Esin_And2018_Cyb	GLTB-101	1	BE12021_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-101	Cytb	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	ACCCCTCCTCATATCCAACCA	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12021_Mgig_Bot2012_COI	GLTB-101	1	BE12021_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	TCTTATTCGTTGGAGACTTTATAACCCT	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12021_Mjap_Gib2022_COI	GLTB-101	1	BE12021_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	AATTTAATTGTGACTAGGCATGGTCTG	151.17671
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12022_Cmae_Bot2011_COI	GLTB-102	1	BE12022_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	ATGAACAGTCTATCCTCCTTTAG	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12022_Hsan_Wil2021_COI	GLTB-102	1	BE12022_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	CCTGGGCCGGTATAGTAGGT	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12022_Mros_Gib2022_COI	GLTB-102	1	BE12022_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12022_Msal_Bot2012_COI	GLTB-102	1	BE12022_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	GYTAGTTCCRATGATGTTAGCTG	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12022_Mstr_Wil2021_COI	GLTB-102	1	BE12022_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	GTAGTGGTAACTACTCATGCTTTA	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12022_Pvir_Dia2013_COI	GLTB-102	1	BE12022_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	CTTAGTGGCATTAATTCGDAATCC	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12022_Rhar_Sim2018_COI	GLTB-102	1	BE12022_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	CTGCTGCTATTGCTCATG	151.17257
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12024_Aamu_Bax2006_COI	GLTB-104	1	BE12024_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	GCACAACCGGGATCTTTACTTC	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12024_Pcan_Bot2011_IGS	GLTB-104	1	BE12024_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-104	IGS	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	CGTAATCCTCAGTACTGGCTA	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12024_Pper_Dia2013_COI	GLTB-104	1	BE12024_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	CTTAGTGGCATTAATTCGDAATCC	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12024_Sspa_Oph2007_COI	GLTB-104	1	BE12024_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	GAAGGCCTTCCGATGATC	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12024_Upin_Bot2011_COI	GLTB-104	1	BE12024_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	CTTTAATTACAGCGTTTTTATTGTTGT	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12024_Vgib_Wil2025_COI	GLTB-104	1	BE12024_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	CCCCTCTTTACAAGTTCTTTAGGGT	151.17575
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12025_Asen_Wil2025_COI	GLTB-105	1	BE12025_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	GTTGTTGTTACAACACATGCTCTTTTG	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12025_Cjap_Sim2018_COI	GLTB-105	1	BE12025_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12025_Esin_And2018_Cyb	GLTB-105	1	BE12025_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-105	Cytb	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	ACCCCTCCTCATATCCAACCA	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12025_Mgig_Bot2012_COI	GLTB-105	1	BE12025_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	TCTTATTCGTTGGAGACTTTATAACCCT	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12025_Mjap_Gib2022_COI	GLTB-105	1	BE12025_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	AATTTAATTGTGACTAGGCATGGTCTG	151.17319
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32693_Aamu_Bax2006_COI	CNSB-002	1	BE32693_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	GCACAACCGGGATCTTTACTTC	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32693_Pcan_Bot2011_IGS	CNSB-002	1	BE32693_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-002	IGS	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	CGTAATCCTCAGTACTGGCTA	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32693_Pper_Dia2013_COI	CNSB-002	1	BE32693_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	CTTAGTGGCATTAATTCGDAATCC	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32693_Sspa_Oph2007_COI	CNSB-002	1	BE32693_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	GAAGGCCTTCCGATGATC	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32693_Upin_Bot2011_COI	CNSB-002	1	BE32693_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	CTTTAATTACAGCGTTTTTATTGTTGT	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32693_Vgib_Wil2025_COI	CNSB-002	1	BE32693_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	CCCCTCTTTACAAGTTCTTTAGGGT	145.77343
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32694_Asen_Wil2025_COI	CNSB-003	1	BE32694_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	GTTGTTGTTACAACACATGCTCTTTTG	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32694_Cjap_Sim2018_COI	CNSB-003	1	BE32694_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32694_Esin_And2018_Cyb	CNSB-003	1	BE32694_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-003	Cytb	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	ACCCCTCCTCATATCCAACCA	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32694_Mgig_Bot2012_COI	CNSB-003	1	BE32694_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	TCTTATTCGTTGGAGACTTTATAACCCT	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32694_Mjap_Gib2022_COI	CNSB-003	1	BE32694_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	AATTTAATTGTGACTAGGCATGGTCTG	145.7747
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32695_Cmae_Bot2011_COI	CNSB-004	1	BE32695_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	ATGAACAGTCTATCCTCCTTTAG	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32695_Hsan_Wil2021_COI	CNSB-004	1	BE32695_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	CCTGGGCCGGTATAGTAGGT	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32695_Mros_Gib2022_COI	CNSB-004	1	BE32695_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32695_Msal_Bot2012_COI	CNSB-004	1	BE32695_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	GYTAGTTCCRATGATGTTAGCTG	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32695_Mstr_Wil2021_COI	CNSB-004	1	BE32695_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	GTAGTGGTAACTACTCATGCTTTA	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32695_Pvir_Dia2013_COI	CNSB-004	1	BE32695_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	CTTAGTGGCATTAATTCGDAATCC	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32695_Rhar_Sim2018_COI	CNSB-004	1	BE32695_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	CTGCTGCTATTGCTCATG	145.7763
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32697_Aamu_Bax2006_COI	CNSB-006	1	BE32697_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	GCACAACCGGGATCTTTACTTC	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32697_Pcan_Bot2011_IGS	CNSB-006	1	BE32697_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-006	IGS	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	CGTAATCCTCAGTACTGGCTA	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32697_Pper_Dia2013_COI	CNSB-006	1	BE32697_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	CTTAGTGGCATTAATTCGDAATCC	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32697_Sspa_Oph2007_COI	CNSB-006	1	BE32697_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	GAAGGCCTTCCGATGATC	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32697_Upin_Bot2011_COI	CNSB-006	1	BE32697_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	CTTTAATTACAGCGTTTTTATTGTTGT	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32697_Vgib_Wil2025_COI	CNSB-006	1	BE32697_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	CCCCTCTTTACAAGTTCTTTAGGGT	145.7822
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32698_Asen_Wil2025_COI	CNSB-007	1	BE32698_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	GTTGTTGTTACAACACATGCTCTTTTG	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32698_Cjap_Sim2018_COI	CNSB-007	1	BE32698_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32698_Esin_And2018_Cyb	CNSB-007	1	BE32698_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-007	Cytb	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	ACCCCTCCTCATATCCAACCA	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32698_Mgig_Bot2012_COI	CNSB-007	1	BE32698_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	TCTTATTCGTTGGAGACTTTATAACCCT	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32698_Mjap_Gib2022_COI	CNSB-007	1	BE32698_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	AATTTAATTGTGACTAGGCATGGTCTG	145.78164
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32699_Cmae_Bot2011_COI	CNSB-008	1	BE32699_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	ATGAACAGTCTATCCTCCTTTAG	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32699_Hsan_Wil2021_COI	CNSB-008	1	BE32699_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	CCTGGGCCGGTATAGTAGGT	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32699_Mros_Gib2022_COI	CNSB-008	1	BE32699_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32699_Msal_Bot2012_COI	CNSB-008	1	BE32699_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	GYTAGTTCCRATGATGTTAGCTG	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32699_Mstr_Wil2021_COI	CNSB-008	1	BE32699_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	GTAGTGGTAACTACTCATGCTTTA	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32699_Pvir_Dia2013_COI	CNSB-008	1	BE32699_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	CTTAGTGGCATTAATTCGDAATCC	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32699_Rhar_Sim2018_COI	CNSB-008	1	BE32699_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	CTGCTGCTATTGCTCATG	145.78279
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32701_Aamu_Bax2006_COI	CNSB-010	1	BE32701_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	GCACAACCGGGATCTTTACTTC	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32701_Pcan_Bot2011_IGS	CNSB-010	1	BE32701_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-010	IGS	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	CGTAATCCTCAGTACTGGCTA	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32701_Pper_Dia2013_COI	CNSB-010	1	BE32701_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	CTTAGTGGCATTAATTCGDAATCC	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32701_Sspa_Oph2007_COI	CNSB-010	1	BE32701_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	GAAGGCCTTCCGATGATC	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32701_Upin_Bot2011_COI	CNSB-010	1	BE32701_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	CTTTAATTACAGCGTTTTTATTGTTGT	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32701_Vgib_Wil2025_COI	CNSB-010	1	BE32701_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	CCCCTCTTTACAAGTTCTTTAGGGT	145.78101
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32702_Asen_Wil2025_COI	CNSB-011	1	BE32702_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	GTTGTTGTTACAACACATGCTCTTTTG	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32702_Cjap_Sim2018_COI	CNSB-011	1	BE32702_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32702_Esin_And2018_Cyb	CNSB-011	1	BE32702_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-011	Cytb	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	ACCCCTCCTCATATCCAACCA	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32702_Mgig_Bot2012_COI	CNSB-011	1	BE32702_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	TCTTATTCGTTGGAGACTTTATAACCCT	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32702_Mjap_Gib2022_COI	CNSB-011	1	BE32702_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	AATTTAATTGTGACTAGGCATGGTCTG	145.78247
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32703_Cmae_Bot2011_COI	CNSB-012	1	BE32703_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	ATGAACAGTCTATCCTCCTTTAG	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32703_Hsan_Wil2021_COI	CNSB-012	1	BE32703_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	CCTGGGCCGGTATAGTAGGT	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32703_Mros_Gib2022_COI	CNSB-012	1	BE32703_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32703_Msal_Bot2012_COI	CNSB-012	1	BE32703_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	GYTAGTTCCRATGATGTTAGCTG	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32703_Mstr_Wil2021_COI	CNSB-012	1	BE32703_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	GTAGTGGTAACTACTCATGCTTTA	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32703_Pvir_Dia2013_COI	CNSB-012	1	BE32703_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	CTTAGTGGCATTAATTCGDAATCC	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32703_Rhar_Sim2018_COI	CNSB-012	1	BE32703_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	CTGCTGCTATTGCTCATG	145.78168
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32705_Aamu_Bax2006_COI	CNSB-014	1	BE32705_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	GCACAACCGGGATCTTTACTTC	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32705_Pcan_Bot2011_IGS	CNSB-014	1	BE32705_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-014	IGS	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	CGTAATCCTCAGTACTGGCTA	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32705_Pper_Dia2013_COI	CNSB-014	1	BE32705_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	CTTAGTGGCATTAATTCGDAATCC	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32705_Sspa_Oph2007_COI	CNSB-014	1	BE32705_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	GAAGGCCTTCCGATGATC	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32705_Upin_Bot2011_COI	CNSB-014	1	BE32705_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	CTTTAATTACAGCGTTTTTATTGTTGT	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32705_Vgib_Wil2025_COI	CNSB-014	1	BE32705_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	CCCCTCTTTACAAGTTCTTTAGGGT	145.78204
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32706_Asen_Wil2025_COI	CNSB-015	1	BE32706_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	GTTGTTGTTACAACACATGCTCTTTTG	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32706_Cjap_Sim2018_COI	CNSB-015	1	BE32706_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32706_Esin_And2018_Cyb	CNSB-015	1	BE32706_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-015	Cytb	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	ACCCCTCCTCATATCCAACCA	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32706_Mgig_Bot2012_COI	CNSB-015	1	BE32706_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	TCTTATTCGTTGGAGACTTTATAACCCT	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32706_Mjap_Gib2022_COI	CNSB-015	1	BE32706_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	AATTTAATTGTGACTAGGCATGGTCTG	145.78114
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32707_Cmae_Bot2011_COI	CNSB-016	1	BE32707_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	ATGAACAGTCTATCCTCCTTTAG	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32707_Hsan_Wil2021_COI	CNSB-016	1	BE32707_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	CCTGGGCCGGTATAGTAGGT	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32707_Mros_Gib2022_COI	CNSB-016	1	BE32707_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32707_Msal_Bot2012_COI	CNSB-016	1	BE32707_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	GYTAGTTCCRATGATGTTAGCTG	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32707_Mstr_Wil2021_COI	CNSB-016	1	BE32707_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	GTAGTGGTAACTACTCATGCTTTA	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32707_Pvir_Dia2013_COI	CNSB-016	1	BE32707_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	CTTAGTGGCATTAATTCGDAATCC	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32707_Rhar_Sim2018_COI	CNSB-016	1	BE32707_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	CTGCTGCTATTGCTCATG	145.78074
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32709_Aamu_Bax2006_COI	CNSB-018	1	BE32709_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	GCACAACCGGGATCTTTACTTC	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32709_Pcan_Bot2011_IGS	CNSB-018	1	BE32709_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-018	IGS	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	CGTAATCCTCAGTACTGGCTA	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32709_Pper_Dia2013_COI	CNSB-018	1	BE32709_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	CTTAGTGGCATTAATTCGDAATCC	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32709_Sspa_Oph2007_COI	CNSB-018	1	BE32709_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	GAAGGCCTTCCGATGATC	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32709_Upin_Bot2011_COI	CNSB-018	1	BE32709_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	CTTTAATTACAGCGTTTTTATTGTTGT	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32709_Vgib_Wil2025_COI	CNSB-018	1	BE32709_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	CCCCTCTTTACAAGTTCTTTAGGGT	145.78124
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32710_Cmae_Bot2011_COI	CNSB-019	1	BE32710_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	ATGAACAGTCTATCCTCCTTTAG	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32710_Hsan_Wil2021_COI	CNSB-019	1	BE32710_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	CCTGGGCCGGTATAGTAGGT	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32710_Mros_Gib2022_COI	CNSB-019	1	BE32710_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32710_Msal_Bot2012_COI	CNSB-019	1	BE32710_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	GYTAGTTCCRATGATGTTAGCTG	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32710_Mstr_Wil2021_COI	CNSB-019	1	BE32710_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	GTAGTGGTAACTACTCATGCTTTA	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32710_Pvir_Dia2013_COI	CNSB-019	1	BE32710_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	CTTAGTGGCATTAATTCGDAATCC	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32710_Rhar_Sim2018_COI	CNSB-019	1	BE32710_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	CTGCTGCTATTGCTCATG	145.78283
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32712_Aamu_Bax2006_COI	CNSB-021	1	BE32712_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	GCACAACCGGGATCTTTACTTC	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32712_Pcan_Bot2011_IGS	CNSB-021	1	BE32712_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-021	IGS	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	CGTAATCCTCAGTACTGGCTA	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32712_Pper_Dia2013_COI	CNSB-021	1	BE32712_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	CTTAGTGGCATTAATTCGDAATCC	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32712_Sspa_Oph2007_COI	CNSB-021	1	BE32712_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	GAAGGCCTTCCGATGATC	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32712_Upin_Bot2011_COI	CNSB-021	1	BE32712_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	CTTTAATTACAGCGTTTTTATTGTTGT	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32712_Vgib_Wil2025_COI	CNSB-021	1	BE32712_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	CCCCTCTTTACAAGTTCTTTAGGGT	145.78355
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32713_Asen_Wil2025_COI	CNSB-022	1	BE32713_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	GTTGTTGTTACAACACATGCTCTTTTG	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32713_Cjap_Sim2018_COI	CNSB-022	1	BE32713_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32713_Esin_And2018_Cyb	CNSB-022	1	BE32713_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-022	Cytb	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	ACCCCTCCTCATATCCAACCA	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32713_Mgig_Bot2012_COI	CNSB-022	1	BE32713_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	TCTTATTCGTTGGAGACTTTATAACCCT	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32713_Mjap_Gib2022_COI	CNSB-022	1	BE32713_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	AATTTAATTGTGACTAGGCATGGTCTG	145.78269
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32714_Cmae_Bot2011_COI	CNSB-023	1	BE32714_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	ATGAACAGTCTATCCTCCTTTAG	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32714_Hsan_Wil2021_COI	CNSB-023	1	BE32714_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	CCTGGGCCGGTATAGTAGGT	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32714_Mros_Gib2022_COI	CNSB-023	1	BE32714_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32714_Msal_Bot2012_COI	CNSB-023	1	BE32714_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	GYTAGTTCCRATGATGTTAGCTG	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32714_Mstr_Wil2021_COI	CNSB-023	1	BE32714_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	GTAGTGGTAACTACTCATGCTTTA	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32714_Pvir_Dia2013_COI	CNSB-023	1	BE32714_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	CTTAGTGGCATTAATTCGDAATCC	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32714_Rhar_Sim2018_COI	CNSB-023	1	BE32714_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	CTGCTGCTATTGCTCATG	145.78405
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32716_Aamu_Bax2006_COI	CNSB-025	1	BE32716_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	GCACAACCGGGATCTTTACTTC	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32716_Pcan_Bot2011_IGS	CNSB-025	1	BE32716_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-025	IGS	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	CGTAATCCTCAGTACTGGCTA	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32716_Pper_Dia2013_COI	CNSB-025	1	BE32716_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	CTTAGTGGCATTAATTCGDAATCC	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32716_Sspa_Oph2007_COI	CNSB-025	1	BE32716_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	GAAGGCCTTCCGATGATC	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32716_Upin_Bot2011_COI	CNSB-025	1	BE32716_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	CTTTAATTACAGCGTTTTTATTGTTGT	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32716_Vgib_Wil2025_COI	CNSB-025	1	BE32716_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	CCCCTCTTTACAAGTTCTTTAGGGT	145.78279
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32717_Asen_Wil2025_COI	CNSB-026	1	BE32717_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	GTTGTTGTTACAACACATGCTCTTTTG	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32717_Cjap_Sim2018_COI	CNSB-026	1	BE32717_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32717_Esin_And2018_Cyb	CNSB-026	1	BE32717_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-026	Cytb	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	ACCCCTCCTCATATCCAACCA	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32717_Mgig_Bot2012_COI	CNSB-026	1	BE32717_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	TCTTATTCGTTGGAGACTTTATAACCCT	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32717_Mjap_Gib2022_COI	CNSB-026	1	BE32717_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	AATTTAATTGTGACTAGGCATGGTCTG	145.78234
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32718_Cmae_Bot2011_COI	CNSB-027	1	BE32718_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	ATGAACAGTCTATCCTCCTTTAG	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32718_Hsan_Wil2021_COI	CNSB-027	1	BE32718_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	CCTGGGCCGGTATAGTAGGT	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32718_Mros_Gib2022_COI	CNSB-027	1	BE32718_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32718_Msal_Bot2012_COI	CNSB-027	1	BE32718_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	GYTAGTTCCRATGATGTTAGCTG	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32718_Mstr_Wil2021_COI	CNSB-027	1	BE32718_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	GTAGTGGTAACTACTCATGCTTTA	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32718_Pvir_Dia2013_COI	CNSB-027	1	BE32718_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	CTTAGTGGCATTAATTCGDAATCC	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32718_Rhar_Sim2018_COI	CNSB-027	1	BE32718_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	CTGCTGCTATTGCTCATG	145.78385
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32720_Asen_Wil2025_COI	CNSB-029	1	BE32720_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	GTTGTTGTTACAACACATGCTCTTTTG	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32720_Cjap_Sim2018_COI	CNSB-029	1	BE32720_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32720_Esin_And2018_Cyb	CNSB-029	1	BE32720_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-029	Cytb	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	ACCCCTCCTCATATCCAACCA	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32720_Mgig_Bot2012_COI	CNSB-029	1	BE32720_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	TCTTATTCGTTGGAGACTTTATAACCCT	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32720_Mjap_Gib2022_COI	CNSB-029	1	BE32720_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	AATTTAATTGTGACTAGGCATGGTCTG	145.78461
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32721_Cmae_Bot2011_COI	CNSB-030	1	BE32721_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	ATGAACAGTCTATCCTCCTTTAG	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32721_Hsan_Wil2021_COI	CNSB-030	1	BE32721_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	CCTGGGCCGGTATAGTAGGT	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32721_Mros_Gib2022_COI	CNSB-030	1	BE32721_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32721_Msal_Bot2012_COI	CNSB-030	1	BE32721_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	GYTAGTTCCRATGATGTTAGCTG	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32721_Mstr_Wil2021_COI	CNSB-030	1	BE32721_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	GTAGTGGTAACTACTCATGCTTTA	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32721_Pvir_Dia2013_COI	CNSB-030	1	BE32721_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	CTTAGTGGCATTAATTCGDAATCC	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32721_Rhar_Sim2018_COI	CNSB-030	1	BE32721_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	CTGCTGCTATTGCTCATG	145.78369
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32723_Aamu_Bax2006_COI	CNSB-032	1	BE32723_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	GCACAACCGGGATCTTTACTTC	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32723_Pcan_Bot2011_IGS	CNSB-032	1	BE32723_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-032	IGS	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	CGTAATCCTCAGTACTGGCTA	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32723_Pper_Dia2013_COI	CNSB-032	1	BE32723_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	CTTAGTGGCATTAATTCGDAATCC	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32723_Sspa_Oph2007_COI	CNSB-032	1	BE32723_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	GAAGGCCTTCCGATGATC	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32723_Upin_Bot2011_COI	CNSB-032	1	BE32723_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	CTTTAATTACAGCGTTTTTATTGTTGT	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32723_Vgib_Wil2025_COI	CNSB-032	1	BE32723_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	CCCCTCTTTACAAGTTCTTTAGGGT	145.78453
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32724_Asen_Wil2025_COI	CNSB-033	1	BE32724_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	GTTGTTGTTACAACACATGCTCTTTTG	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32724_Cjap_Sim2018_COI	CNSB-033	1	BE32724_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32724_Esin_And2018_Cyb	CNSB-033	1	BE32724_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-033	Cytb	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	ACCCCTCCTCATATCCAACCA	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32724_Mjap_Gib2022_COI	CNSB-033	1	BE32724_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	AATTTAATTGTGACTAGGCATGGTCTG	145.78517
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32725_Cmae_Bot2011_COI	CNSB-034	1	BE32725_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	ATGAACAGTCTATCCTCCTTTAG	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32725_Hsan_Wil2021_COI	CNSB-034	1	BE32725_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	CCTGGGCCGGTATAGTAGGT	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32725_Mros_Gib2022_COI	CNSB-034	1	BE32725_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32725_Msal_Bot2012_COI	CNSB-034	1	BE32725_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	GYTAGTTCCRATGATGTTAGCTG	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32725_Mstr_Wil2021_COI	CNSB-034	1	BE32725_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	GTAGTGGTAACTACTCATGCTTTA	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32725_Pvir_Dia2013_COI	CNSB-034	1	BE32725_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	CTTAGTGGCATTAATTCGDAATCC	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32725_Rhar_Sim2018_COI	CNSB-034	1	BE32725_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	CTGCTGCTATTGCTCATG	145.78422
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32734_Aamu_Bax2006_COI	TSVB-002	1	BE32734_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	GCACAACCGGGATCTTTACTTC	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32734_Pcan_Bot2011_IGS	TSVB-002	1	BE32734_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-002	IGS	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	CGTAATCCTCAGTACTGGCTA	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32734_Pper_Dia2013_COI	TSVB-002	1	BE32734_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	CTTAGTGGCATTAATTCGDAATCC	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32734_Sspa_Oph2007_COI	TSVB-002	1	BE32734_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	GAAGGCCTTCCGATGATC	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32734_Upin_Bot2011_COI	TSVB-002	1	BE32734_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	CTTTAATTACAGCGTTTTTATTGTTGT	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32734_Vgib_Wil2025_COI	TSVB-002	1	BE32734_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	CCCCTCTTTACAAGTTCTTTAGGGT	146.84133
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32735_Asen_Wil2025_COI	TSVB-003	1	BE32735_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	GTTGTTGTTACAACACATGCTCTTTTG	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32735_Cjap_Sim2018_COI	TSVB-003	1	BE32735_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32735_Esin_And2018_Cyb	TSVB-003	1	BE32735_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-003	Cytb	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	ACCCCTCCTCATATCCAACCA	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32735_Mgig_Bot2012_COI	TSVB-003	1	BE32735_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	TCTTATTCGTTGGAGACTTTATAACCCT	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32735_Mjap_Gib2022_COI	TSVB-003	1	BE32735_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	AATTTAATTGTGACTAGGCATGGTCTG	146.84007
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32736_Cmae_Bot2011_COI	TSVB-004	1	BE32736_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	ATGAACAGTCTATCCTCCTTTAG	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32736_Hsan_Wil2021_COI	TSVB-004	1	BE32736_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	CCTGGGCCGGTATAGTAGGT	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32736_Mros_Gib2022_COI	TSVB-004	1	BE32736_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32736_Msal_Bot2012_COI	TSVB-004	1	BE32736_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	GYTAGTTCCRATGATGTTAGCTG	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32736_Mstr_Wil2021_COI	TSVB-004	1	BE32736_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	GTAGTGGTAACTACTCATGCTTTA	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32736_Pvir_Dia2013_COI	TSVB-004	1	BE32736_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	CTTAGTGGCATTAATTCGDAATCC	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32736_Rhar_Sim2018_COI	TSVB-004	1	BE32736_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	CTGCTGCTATTGCTCATG	146.8404
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32738_Aamu_Bax2006_COI	TSVB-006	1	BE32738_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	GCACAACCGGGATCTTTACTTC	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32738_Pcan_Bot2011_IGS	TSVB-006	1	BE32738_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-006	IGS	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	CGTAATCCTCAGTACTGGCTA	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32738_Pper_Dia2013_COI	TSVB-006	1	BE32738_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	CTTAGTGGCATTAATTCGDAATCC	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32738_Sspa_Oph2007_COI	TSVB-006	1	BE32738_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	GAAGGCCTTCCGATGATC	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32738_Upin_Bot2011_COI	TSVB-006	1	BE32738_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	CTTTAATTACAGCGTTTTTATTGTTGT	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32738_Vgib_Wil2025_COI	TSVB-006	1	BE32738_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	CCCCTCTTTACAAGTTCTTTAGGGT	146.84104
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32739_Asen_Wil2025_COI	TSVB-007	1	BE32739_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	GTTGTTGTTACAACACATGCTCTTTTG	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32739_Cjap_Sim2018_COI	TSVB-007	1	BE32739_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32739_Esin_And2018_Cyb	TSVB-007	1	BE32739_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-007	Cytb	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	ACCCCTCCTCATATCCAACCA	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32739_Mgig_Bot2012_COI	TSVB-007	1	BE32739_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	TCTTATTCGTTGGAGACTTTATAACCCT	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32739_Mjap_Gib2022_COI	TSVB-007	1	BE32739_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	AATTTAATTGTGACTAGGCATGGTCTG	146.8396
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32741_Aamu_Bax2006_COI	TSVB-009	1	BE32741_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	GCACAACCGGGATCTTTACTTC	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32741_Pcan_Bot2011_IGS	TSVB-009	1	BE32741_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-009	IGS	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	CGTAATCCTCAGTACTGGCTA	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32741_Pper_Dia2013_COI	TSVB-009	1	BE32741_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	CTTAGTGGCATTAATTCGDAATCC	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32741_Sspa_Oph2007_COI	TSVB-009	1	BE32741_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	GAAGGCCTTCCGATGATC	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32741_Upin_Bot2011_COI	TSVB-009	1	BE32741_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	CTTTAATTACAGCGTTTTTATTGTTGT	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32741_Vgib_Wil2025_COI	TSVB-009	1	BE32741_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	CCCCTCTTTACAAGTTCTTTAGGGT	146.84027
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32742_Asen_Wil2025_COI	TSVB-010	1	BE32742_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	GTTGTTGTTACAACACATGCTCTTTTG	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32742_Cjap_Sim2018_COI	TSVB-010	1	BE32742_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32742_Esin_And2018_Cyb	TSVB-010	1	BE32742_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-010	Cytb	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	ACCCCTCCTCATATCCAACCA	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32742_Mgig_Bot2012_COI	TSVB-010	1	BE32742_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	TCTTATTCGTTGGAGACTTTATAACCCT	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32742_Mjap_Gib2022_COI	TSVB-010	1	BE32742_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	AATTTAATTGTGACTAGGCATGGTCTG	146.83856
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32743_Cmae_Bot2011_COI	TSVB-011	1	BE32743_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	ATGAACAGTCTATCCTCCTTTAG	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32743_Hsan_Wil2021_COI	TSVB-011	1	BE32743_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	CCTGGGCCGGTATAGTAGGT	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32743_Mros_Gib2022_COI	TSVB-011	1	BE32743_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32743_Msal_Bot2012_COI	TSVB-011	1	BE32743_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	GYTAGTTCCRATGATGTTAGCTG	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32743_Mstr_Wil2021_COI	TSVB-011	1	BE32743_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	GTAGTGGTAACTACTCATGCTTTA	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32743_Pvir_Dia2013_COI	TSVB-011	1	BE32743_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	CTTAGTGGCATTAATTCGDAATCC	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32743_Rhar_Sim2018_COI	TSVB-011	1	BE32743_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	CTGCTGCTATTGCTCATG	146.83706
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32745_Aamu_Bax2006_COI	TSVB-013	1	BE32745_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	GCACAACCGGGATCTTTACTTC	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32745_Pcan_Bot2011_IGS	TSVB-013	1	BE32745_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-013	IGS	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	CGTAATCCTCAGTACTGGCTA	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32745_Pper_Dia2013_COI	TSVB-013	1	BE32745_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	CTTAGTGGCATTAATTCGDAATCC	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32745_Sspa_Oph2007_COI	TSVB-013	1	BE32745_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	GAAGGCCTTCCGATGATC	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32745_Upin_Bot2011_COI	TSVB-013	1	BE32745_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	CTTTAATTACAGCGTTTTTATTGTTGT	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32745_Vgib_Wil2025_COI	TSVB-013	1	BE32745_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	CCCCTCTTTACAAGTTCTTTAGGGT	146.83377
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32746_Asen_Wil2025_COI	TSVB-014	1	BE32746_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	GTTGTTGTTACAACACATGCTCTTTTG	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32746_Cjap_Sim2018_COI	TSVB-014	1	BE32746_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32746_Esin_And2018_Cyb	TSVB-014	1	BE32746_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-014	Cytb	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	ACCCCTCCTCATATCCAACCA	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32746_Mgig_Bot2012_COI	TSVB-014	1	BE32746_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	TCTTATTCGTTGGAGACTTTATAACCCT	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32746_Mjap_Gib2022_COI	TSVB-014	1	BE32746_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	AATTTAATTGTGACTAGGCATGGTCTG	146.83263
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32747_Cmae_Bot2011_COI	TSVB-015	1	BE32747_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	ATGAACAGTCTATCCTCCTTTAG	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32747_Hsan_Wil2021_COI	TSVB-015	1	BE32747_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	CCTGGGCCGGTATAGTAGGT	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32747_Mros_Gib2022_COI	TSVB-015	1	BE32747_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32747_Msal_Bot2012_COI	TSVB-015	1	BE32747_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	GYTAGTTCCRATGATGTTAGCTG	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32747_Mstr_Wil2021_COI	TSVB-015	1	BE32747_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	GTAGTGGTAACTACTCATGCTTTA	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32747_Pvir_Dia2013_COI	TSVB-015	1	BE32747_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	CTTAGTGGCATTAATTCGDAATCC	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32747_Rhar_Sim2018_COI	TSVB-015	1	BE32747_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	CTGCTGCTATTGCTCATG	146.83915
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32749_Aamu_Bax2006_COI	TSVB-017	1	BE32749_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	GCACAACCGGGATCTTTACTTC	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32749_Pcan_Bot2011_IGS	TSVB-017	1	BE32749_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-017	IGS	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	CGTAATCCTCAGTACTGGCTA	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32749_Pper_Dia2013_COI	TSVB-017	1	BE32749_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	CTTAGTGGCATTAATTCGDAATCC	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32749_Sspa_Oph2007_COI	TSVB-017	1	BE32749_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	GAAGGCCTTCCGATGATC	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32749_Upin_Bot2011_COI	TSVB-017	1	BE32749_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	CTTTAATTACAGCGTTTTTATTGTTGT	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32749_Vgib_Wil2025_COI	TSVB-017	1	BE32749_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	CCCCTCTTTACAAGTTCTTTAGGGT	146.83614
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32750_Cmae_Bot2011_COI	TSVB-018	1	BE32750_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	ATGAACAGTCTATCCTCCTTTAG	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32750_Hsan_Wil2021_COI	TSVB-018	1	BE32750_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	CCTGGGCCGGTATAGTAGGT	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32750_Mros_Gib2022_COI	TSVB-018	1	BE32750_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32750_Msal_Bot2012_COI	TSVB-018	1	BE32750_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	GYTAGTTCCRATGATGTTAGCTG	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32750_Mstr_Wil2021_COI	TSVB-018	1	BE32750_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	GTAGTGGTAACTACTCATGCTTTA	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32750_Pvir_Dia2013_COI	TSVB-018	1	BE32750_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	CTTAGTGGCATTAATTCGDAATCC	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32750_Rhar_Sim2018_COI	TSVB-018	1	BE32750_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	CTGCTGCTATTGCTCATG	146.83444
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32752_Aamu_Bax2006_COI	TSVB-020	1	BE32752_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	GCACAACCGGGATCTTTACTTC	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32752_Pcan_Bot2011_IGS	TSVB-020	1	BE32752_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-020	IGS	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	CGTAATCCTCAGTACTGGCTA	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32752_Pper_Dia2013_COI	TSVB-020	1	BE32752_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	CTTAGTGGCATTAATTCGDAATCC	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32752_Sspa_Oph2007_COI	TSVB-020	1	BE32752_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	GAAGGCCTTCCGATGATC	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32752_Upin_Bot2011_COI	TSVB-020	1	BE32752_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	CTTTAATTACAGCGTTTTTATTGTTGT	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32752_Vgib_Wil2025_COI	TSVB-020	1	BE32752_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	CCCCTCTTTACAAGTTCTTTAGGGT	146.83792
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32753_Asen_Wil2025_COI	TSVB-021	1	BE32753_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	GTTGTTGTTACAACACATGCTCTTTTG	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32753_Cjap_Sim2018_COI	TSVB-021	1	BE32753_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32753_Esin_And2018_Cyb	TSVB-021	1	BE32753_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-021	Cytb	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	ACCCCTCCTCATATCCAACCA	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32753_Mgig_Bot2012_COI	TSVB-021	1	BE32753_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	TCTTATTCGTTGGAGACTTTATAACCCT	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32753_Mjap_Gib2022_COI	TSVB-021	1	BE32753_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	AATTTAATTGTGACTAGGCATGGTCTG	146.83614
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32754_Cmae_Bot2011_COI	TSVB-022	1	BE32754_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	ATGAACAGTCTATCCTCCTTTAG	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32754_Hsan_Wil2021_COI	TSVB-022	1	BE32754_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	CCTGGGCCGGTATAGTAGGT	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32754_Mros_Gib2022_COI	TSVB-022	1	BE32754_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32754_Msal_Bot2012_COI	TSVB-022	1	BE32754_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	GYTAGTTCCRATGATGTTAGCTG	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32754_Mstr_Wil2021_COI	TSVB-022	1	BE32754_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	GTAGTGGTAACTACTCATGCTTTA	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32754_Pvir_Dia2013_COI	TSVB-022	1	BE32754_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	CTTAGTGGCATTAATTCGDAATCC	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32754_Rhar_Sim2018_COI	TSVB-022	1	BE32754_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	CTGCTGCTATTGCTCATG	146.83487
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32756_Aamu_Bax2006_COI	TSVB-024	1	BE32756_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	GCACAACCGGGATCTTTACTTC	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32756_Pcan_Bot2011_IGS	TSVB-024	1	BE32756_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-024	IGS	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	CGTAATCCTCAGTACTGGCTA	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32756_Pper_Dia2013_COI	TSVB-024	1	BE32756_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	CTTAGTGGCATTAATTCGDAATCC	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32756_Sspa_Oph2007_COI	TSVB-024	1	BE32756_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	GAAGGCCTTCCGATGATC	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32756_Upin_Bot2011_COI	TSVB-024	1	BE32756_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	CTTTAATTACAGCGTTTTTATTGTTGT	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32756_Vgib_Wil2025_COI	TSVB-024	1	BE32756_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	CCCCTCTTTACAAGTTCTTTAGGGT	146.83443
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32757_Asen_Wil2025_COI	TSVB-025	1	BE32757_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	GTTGTTGTTACAACACATGCTCTTTTG	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32757_Cjap_Sim2018_COI	TSVB-025	1	BE32757_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32757_Esin_And2018_Cyb	TSVB-025	1	BE32757_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-025	Cytb	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	ACCCCTCCTCATATCCAACCA	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32757_Mgig_Bot2012_COI	TSVB-025	1	BE32757_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	TCTTATTCGTTGGAGACTTTATAACCCT	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32757_Mjap_Gib2022_COI	TSVB-025	1	BE32757_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	AATTTAATTGTGACTAGGCATGGTCTG	146.83361
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32758_Cmae_Bot2011_COI	TSVB-026	1	BE32758_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	ATGAACAGTCTATCCTCCTTTAG	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32758_Hsan_Wil2021_COI	TSVB-026	1	BE32758_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	CCTGGGCCGGTATAGTAGGT	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32758_Mros_Gib2022_COI	TSVB-026	1	BE32758_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32758_Msal_Bot2012_COI	TSVB-026	1	BE32758_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	GYTAGTTCCRATGATGTTAGCTG	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32758_Mstr_Wil2021_COI	TSVB-026	1	BE32758_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	GTAGTGGTAACTACTCATGCTTTA	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32758_Pvir_Dia2013_COI	TSVB-026	1	BE32758_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	CTTAGTGGCATTAATTCGDAATCC	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32758_Rhar_Sim2018_COI	TSVB-026	1	BE32758_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	CTGCTGCTATTGCTCATG	146.83467
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32760_Asen_Wil2025_COI	TSVB-028	1	BE32760_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	GTTGTTGTTACAACACATGCTCTTTTG	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32760_Cjap_Sim2018_COI	TSVB-028	1	BE32760_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32760_Esin_And2018_Cyb	TSVB-028	1	BE32760_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-028	Cytb	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	ACCCCTCCTCATATCCAACCA	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32760_Mgig_Bot2012_COI	TSVB-028	1	BE32760_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	TCTTATTCGTTGGAGACTTTATAACCCT	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32760_Mjap_Gib2022_COI	TSVB-028	1	BE32760_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	AATTTAATTGTGACTAGGCATGGTCTG	146.83458
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32761_Cmae_Bot2011_COI	TSVB-029	1	BE32761_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	ATGAACAGTCTATCCTCCTTTAG	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32761_Hsan_Wil2021_COI	TSVB-029	1	BE32761_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	CCTGGGCCGGTATAGTAGGT	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32761_Mros_Gib2022_COI	TSVB-029	1	BE32761_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32761_Msal_Bot2012_COI	TSVB-029	1	BE32761_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	GYTAGTTCCRATGATGTTAGCTG	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32761_Mstr_Wil2021_COI	TSVB-029	1	BE32761_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	GTAGTGGTAACTACTCATGCTTTA	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32761_Pvir_Dia2013_COI	TSVB-029	1	BE32761_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	CTTAGTGGCATTAATTCGDAATCC	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32761_Rhar_Sim2018_COI	TSVB-029	1	BE32761_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	CTGCTGCTATTGCTCATG	146.83352
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32763_Aamu_Bax2006_COI	TSVB-031	1	BE32763_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	GCACAACCGGGATCTTTACTTC	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32763_Pcan_Bot2011_IGS	TSVB-031	1	BE32763_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-031	IGS	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	CGTAATCCTCAGTACTGGCTA	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32763_Pper_Dia2013_COI	TSVB-031	1	BE32763_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	CTTAGTGGCATTAATTCGDAATCC	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32763_Sspa_Oph2007_COI	TSVB-031	1	BE32763_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	GAAGGCCTTCCGATGATC	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32763_Upin_Bot2011_COI	TSVB-031	1	BE32763_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	CTTTAATTACAGCGTTTTTATTGTTGT	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32763_Vgib_Wil2025_COI	TSVB-031	1	BE32763_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	CCCCTCTTTACAAGTTCTTTAGGGT	146.83387
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32764_Asen_Wil2025_COI	TSVB-032	1	BE32764_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	GTTGTTGTTACAACACATGCTCTTTTG	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32764_Cjap_Sim2018_COI	TSVB-032	1	BE32764_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32764_Esin_And2018_Cyb	TSVB-032	1	BE32764_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-032	Cytb	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	ACCCCTCCTCATATCCAACCA	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32764_Mgig_Bot2012_COI	TSVB-032	1	BE32764_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	TCTTATTCGTTGGAGACTTTATAACCCT	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32764_Mjap_Gib2022_COI	TSVB-032	1	BE32764_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	AATTTAATTGTGACTAGGCATGGTCTG	146.83404
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32765_Cmae_Bot2011_COI	TSVB-033	1	BE32765_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	ATGAACAGTCTATCCTCCTTTAG	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32765_Hsan_Wil2021_COI	TSVB-033	1	BE32765_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	CCTGGGCCGGTATAGTAGGT	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32765_Mros_Gib2022_COI	TSVB-033	1	BE32765_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32765_Msal_Bot2012_COI	TSVB-033	1	BE32765_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	GYTAGTTCCRATGATGTTAGCTG	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32765_Mstr_Wil2021_COI	TSVB-033	1	BE32765_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	GTAGTGGTAACTACTCATGCTTTA	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32765_Pvir_Dia2013_COI	TSVB-033	1	BE32765_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	CTTAGTGGCATTAATTCGDAATCC	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32765_Rhar_Sim2018_COI	TSVB-033	1	BE32765_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	CTGCTGCTATTGCTCATG	146.83413
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32767_Aamu_Bax2006_COI	TSVB-035	1	BE32767_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	GCACAACCGGGATCTTTACTTC	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32767_Pcan_Bot2011_IGS	TSVB-035	1	BE32767_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-035	IGS	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	CGTAATCCTCAGTACTGGCTA	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32767_Pper_Dia2013_COI	TSVB-035	1	BE32767_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	CTTAGTGGCATTAATTCGDAATCC	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32767_Sspa_Oph2007_COI	TSVB-035	1	BE32767_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	GAAGGCCTTCCGATGATC	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32767_Upin_Bot2011_COI	TSVB-035	1	BE32767_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	CTTTAATTACAGCGTTTTTATTGTTGT	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32767_Vgib_Wil2025_COI	TSVB-035	1	BE32767_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	CCCCTCTTTACAAGTTCTTTAGGGT	146.83158
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32774_Aamu_Bax2006_COI	DRWB-001	1	BE32774_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	GCACAACCGGGATCTTTACTTC	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32774_Pcan_Bot2011_IGS	DRWB-001	1	BE32774_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-001	IGS	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	CGTAATCCTCAGTACTGGCTA	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32774_Pper_Dia2013_COI	DRWB-001	1	BE32774_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	CTTAGTGGCATTAATTCGDAATCC	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32774_Sspa_Oph2007_COI	DRWB-001	1	BE32774_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	GAAGGCCTTCCGATGATC	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32774_Upin_Bot2011_COI	DRWB-001	1	BE32774_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	CTTTAATTACAGCGTTTTTATTGTTGT	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32774_Vgib_Wil2025_COI	DRWB-001	1	BE32774_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	CCCCTCTTTACAAGTTCTTTAGGGT	130.90592
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32775_Asen_Wil2025_COI	DRWB-002	1	BE32775_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	GTTGTTGTTACAACACATGCTCTTTTG	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32775_Cjap_Sim2018_COI	DRWB-002	1	BE32775_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32775_Esin_And2018_Cyb	DRWB-002	1	BE32775_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-002	Cytb	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	ACCCCTCCTCATATCCAACCA	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32775_Mgig_Bot2012_COI	DRWB-002	1	BE32775_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	TCTTATTCGTTGGAGACTTTATAACCCT	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32775_Mjap_Gib2022_COI	DRWB-002	1	BE32775_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	AATTTAATTGTGACTAGGCATGGTCTG	130.90463
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32776_Cmae_Bot2011_COI	DRWB-003	1	BE32776_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	ATGAACAGTCTATCCTCCTTTAG	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32776_Hsan_Wil2021_COI	DRWB-003	1	BE32776_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	CCTGGGCCGGTATAGTAGGT	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32776_Mros_Gib2022_COI	DRWB-003	1	BE32776_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32776_Msal_Bot2012_COI	DRWB-003	1	BE32776_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	GYTAGTTCCRATGATGTTAGCTG	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32776_Mstr_Wil2021_COI	DRWB-003	1	BE32776_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	GTAGTGGTAACTACTCATGCTTTA	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32776_Pvir_Dia2013_COI	DRWB-003	1	BE32776_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	CTTAGTGGCATTAATTCGDAATCC	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32776_Rhar_Sim2018_COI	DRWB-003	1	BE32776_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	CTGCTGCTATTGCTCATG	130.90357
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32778_Aamu_Bax2006_COI	DRWB-005	1	BE32778_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	GCACAACCGGGATCTTTACTTC	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32778_Pcan_Bot2011_IGS	DRWB-005	1	BE32778_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-005	IGS	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	CGTAATCCTCAGTACTGGCTA	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32778_Pper_Dia2013_COI	DRWB-005	1	BE32778_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	CTTAGTGGCATTAATTCGDAATCC	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32778_Sspa_Oph2007_COI	DRWB-005	1	BE32778_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	GAAGGCCTTCCGATGATC	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32778_Upin_Bot2011_COI	DRWB-005	1	BE32778_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	CTTTAATTACAGCGTTTTTATTGTTGT	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32778_Vgib_Wil2025_COI	DRWB-005	1	BE32778_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	CCCCTCTTTACAAGTTCTTTAGGGT	130.90546
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32779_Asen_Wil2025_COI	DRWB-006	1	BE32779_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	GTTGTTGTTACAACACATGCTCTTTTG	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32779_Cjap_Sim2018_COI	DRWB-006	1	BE32779_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32779_Esin_And2018_Cyb	DRWB-006	1	BE32779_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-006	Cytb	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	ACCCCTCCTCATATCCAACCA	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32779_Mgig_Bot2012_COI	DRWB-006	1	BE32779_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	TCTTATTCGTTGGAGACTTTATAACCCT	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32779_Mjap_Gib2022_COI	DRWB-006	1	BE32779_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	AATTTAATTGTGACTAGGCATGGTCTG	130.90367
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32781_Aamu_Bax2006_COI	DRWB-008	1	BE32781_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	GCACAACCGGGATCTTTACTTC	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32781_Pcan_Bot2011_IGS	DRWB-008	1	BE32781_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-008	IGS	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	CGTAATCCTCAGTACTGGCTA	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32781_Pper_Dia2013_COI	DRWB-008	1	BE32781_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	CTTAGTGGCATTAATTCGDAATCC	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32781_Sspa_Oph2007_COI	DRWB-008	1	BE32781_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	GAAGGCCTTCCGATGATC	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32781_Upin_Bot2011_COI	DRWB-008	1	BE32781_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	CTTTAATTACAGCGTTTTTATTGTTGT	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32781_Vgib_Wil2025_COI	DRWB-008	1	BE32781_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	CCCCTCTTTACAAGTTCTTTAGGGT	130.9043
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32782_Asen_Wil2025_COI	DRWB-009	1	BE32782_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	GTTGTTGTTACAACACATGCTCTTTTG	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32782_Cjap_Sim2018_COI	DRWB-009	1	BE32782_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32782_Esin_And2018_Cyb	DRWB-009	1	BE32782_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-009	Cytb	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	ACCCCTCCTCATATCCAACCA	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32782_Mgig_Bot2012_COI	DRWB-009	1	BE32782_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	TCTTATTCGTTGGAGACTTTATAACCCT	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32782_Mjap_Gib2022_COI	DRWB-009	1	BE32782_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	AATTTAATTGTGACTAGGCATGGTCTG	130.90555
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32783_Cmae_Bot2011_COI	DRWB-010	1	BE32783_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	ATGAACAGTCTATCCTCCTTTAG	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32783_Hsan_Wil2021_COI	DRWB-010	1	BE32783_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	CCTGGGCCGGTATAGTAGGT	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32783_Mros_Gib2022_COI	DRWB-010	1	BE32783_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32783_Msal_Bot2012_COI	DRWB-010	1	BE32783_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	GYTAGTTCCRATGATGTTAGCTG	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32783_Mstr_Wil2021_COI	DRWB-010	1	BE32783_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	GTAGTGGTAACTACTCATGCTTTA	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32783_Pvir_Dia2013_COI	DRWB-010	1	BE32783_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	CTTAGTGGCATTAATTCGDAATCC	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32783_Rhar_Sim2018_COI	DRWB-010	1	BE32783_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	CTGCTGCTATTGCTCATG	130.90673
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32785_Aamu_Bax2006_COI	DRWB-012	1	BE32785_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	GCACAACCGGGATCTTTACTTC	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32785_Pcan_Bot2011_IGS	DRWB-012	1	BE32785_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-012	IGS	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	CGTAATCCTCAGTACTGGCTA	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32785_Pper_Dia2013_COI	DRWB-012	1	BE32785_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	CTTAGTGGCATTAATTCGDAATCC	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32785_Sspa_Oph2007_COI	DRWB-012	1	BE32785_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	GAAGGCCTTCCGATGATC	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32785_Upin_Bot2011_COI	DRWB-012	1	BE32785_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	CTTTAATTACAGCGTTTTTATTGTTGT	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32785_Vgib_Wil2025_COI	DRWB-012	1	BE32785_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	CCCCTCTTTACAAGTTCTTTAGGGT	130.90373
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32786_Asen_Wil2025_COI	DRWB-013	1	BE32786_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	GTTGTTGTTACAACACATGCTCTTTTG	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32786_Cjap_Sim2018_COI	DRWB-013	1	BE32786_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32786_Esin_And2018_Cyb	DRWB-013	1	BE32786_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-013	Cytb	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	ACCCCTCCTCATATCCAACCA	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32786_Mgig_Bot2012_COI	DRWB-013	1	BE32786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	TCTTATTCGTTGGAGACTTTATAACCCT	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32786_Mjap_Gib2022_COI	DRWB-013	1	BE32786_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	AATTTAATTGTGACTAGGCATGGTCTG	130.90491
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32787_Cmae_Bot2011_COI	DRWB-014	1	BE32787_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	ATGAACAGTCTATCCTCCTTTAG	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32787_Hsan_Wil2021_COI	DRWB-014	1	BE32787_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	CCTGGGCCGGTATAGTAGGT	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32787_Mros_Gib2022_COI	DRWB-014	1	BE32787_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32787_Msal_Bot2012_COI	DRWB-014	1	BE32787_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	GYTAGTTCCRATGATGTTAGCTG	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32787_Mstr_Wil2021_COI	DRWB-014	1	BE32787_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	GTAGTGGTAACTACTCATGCTTTA	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32787_Pvir_Dia2013_COI	DRWB-014	1	BE32787_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	CTTAGTGGCATTAATTCGDAATCC	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32787_Rhar_Sim2018_COI	DRWB-014	1	BE32787_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	CTGCTGCTATTGCTCATG	130.90613
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32789_Aamu_Bax2006_COI	DRWB-016	1	BE32789_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	GCACAACCGGGATCTTTACTTC	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32789_Pcan_Bot2011_IGS	DRWB-016	1	BE32789_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-016	IGS	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	CGTAATCCTCAGTACTGGCTA	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32789_Pper_Dia2013_COI	DRWB-016	1	BE32789_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	CTTAGTGGCATTAATTCGDAATCC	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32789_Sspa_Oph2007_COI	DRWB-016	1	BE32789_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	GAAGGCCTTCCGATGATC	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32789_Upin_Bot2011_COI	DRWB-016	1	BE32789_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	CTTTAATTACAGCGTTTTTATTGTTGT	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32789_Vgib_Wil2025_COI	DRWB-016	1	BE32789_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	CCCCTCTTTACAAGTTCTTTAGGGT	130.90842
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32790_Cmae_Bot2011_COI	DRWB-017	1	BE32790_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	ATGAACAGTCTATCCTCCTTTAG	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32790_Hsan_Wil2021_COI	DRWB-017	1	BE32790_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	CCTGGGCCGGTATAGTAGGT	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32790_Mros_Gib2022_COI	DRWB-017	1	BE32790_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32790_Msal_Bot2012_COI	DRWB-017	1	BE32790_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	GYTAGTTCCRATGATGTTAGCTG	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32790_Mstr_Wil2021_COI	DRWB-017	1	BE32790_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	GTAGTGGTAACTACTCATGCTTTA	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32790_Pvir_Dia2013_COI	DRWB-017	1	BE32790_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	CTTAGTGGCATTAATTCGDAATCC	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32790_Rhar_Sim2018_COI	DRWB-017	1	BE32790_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	CTGCTGCTATTGCTCATG	130.90959
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32792_Aamu_Bax2006_COI	DRWB-019	1	BE32792_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	GCACAACCGGGATCTTTACTTC	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32792_Pcan_Bot2011_IGS	DRWB-019	1	BE32792_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-019	IGS	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	CGTAATCCTCAGTACTGGCTA	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32792_Pper_Dia2013_COI	DRWB-019	1	BE32792_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	CTTAGTGGCATTAATTCGDAATCC	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32792_Sspa_Oph2007_COI	DRWB-019	1	BE32792_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	GAAGGCCTTCCGATGATC	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32792_Upin_Bot2011_COI	DRWB-019	1	BE32792_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	CTTTAATTACAGCGTTTTTATTGTTGT	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32792_Vgib_Wil2025_COI	DRWB-019	1	BE32792_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	CCCCTCTTTACAAGTTCTTTAGGGT	130.91205
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32793_Asen_Wil2025_COI	DRWB-020	1	BE32793_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	GTTGTTGTTACAACACATGCTCTTTTG	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32793_Cjap_Sim2018_COI	DRWB-020	1	BE32793_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32793_Esin_And2018_Cyb	DRWB-020	1	BE32793_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-020	Cytb	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	ACCCCTCCTCATATCCAACCA	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32793_Mgig_Bot2012_COI	DRWB-020	1	BE32793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	TCTTATTCGTTGGAGACTTTATAACCCT	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32793_Mjap_Gib2022_COI	DRWB-020	1	BE32793_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	AATTTAATTGTGACTAGGCATGGTCTG	130.91324
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32794_Cmae_Bot2011_COI	DRWB-021	1	BE32794_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	ATGAACAGTCTATCCTCCTTTAG	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32794_Hsan_Wil2021_COI	DRWB-021	1	BE32794_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	CCTGGGCCGGTATAGTAGGT	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32794_Mros_Gib2022_COI	DRWB-021	1	BE32794_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32794_Msal_Bot2012_COI	DRWB-021	1	BE32794_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	GYTAGTTCCRATGATGTTAGCTG	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32794_Mstr_Wil2021_COI	DRWB-021	1	BE32794_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	GTAGTGGTAACTACTCATGCTTTA	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32794_Pvir_Dia2013_COI	DRWB-021	1	BE32794_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	CTTAGTGGCATTAATTCGDAATCC	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32794_Rhar_Sim2018_COI	DRWB-021	1	BE32794_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	CTGCTGCTATTGCTCATG	130.90562
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32796_Aamu_Bax2006_COI	DRWB-023	1	BE32796_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	GCACAACCGGGATCTTTACTTC	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32796_Pcan_Bot2011_IGS	DRWB-023	1	BE32796_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-023	IGS	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	CGTAATCCTCAGTACTGGCTA	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32796_Pper_Dia2013_COI	DRWB-023	1	BE32796_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	CTTAGTGGCATTAATTCGDAATCC	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32796_Sspa_Oph2007_COI	DRWB-023	1	BE32796_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	GAAGGCCTTCCGATGATC	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32796_Upin_Bot2011_COI	DRWB-023	1	BE32796_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	CTTTAATTACAGCGTTTTTATTGTTGT	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32796_Vgib_Wil2025_COI	DRWB-023	1	BE32796_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	CCCCTCTTTACAAGTTCTTTAGGGT	130.90799
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32797_Asen_Wil2025_COI	DRWB-024	1	BE32797_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	GTTGTTGTTACAACACATGCTCTTTTG	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32797_Cjap_Sim2018_COI	DRWB-024	1	BE32797_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32797_Esin_And2018_Cyb	DRWB-024	1	BE32797_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-024	Cytb	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	ACCCCTCCTCATATCCAACCA	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32797_Mgig_Bot2012_COI	DRWB-024	1	BE32797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	TCTTATTCGTTGGAGACTTTATAACCCT	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32797_Mjap_Gib2022_COI	DRWB-024	1	BE32797_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	AATTTAATTGTGACTAGGCATGGTCTG	130.90914
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32798_Cmae_Bot2011_COI	DRWB-025	1	BE32798_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	ATGAACAGTCTATCCTCCTTTAG	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32798_Hsan_Wil2021_COI	DRWB-025	1	BE32798_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	CCTGGGCCGGTATAGTAGGT	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32798_Mros_Gib2022_COI	DRWB-025	1	BE32798_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32798_Msal_Bot2012_COI	DRWB-025	1	BE32798_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	GYTAGTTCCRATGATGTTAGCTG	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32798_Mstr_Wil2021_COI	DRWB-025	1	BE32798_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	GTAGTGGTAACTACTCATGCTTTA	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32798_Pvir_Dia2013_COI	DRWB-025	1	BE32798_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	CTTAGTGGCATTAATTCGDAATCC	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32798_Rhar_Sim2018_COI	DRWB-025	1	BE32798_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	CTGCTGCTATTGCTCATG	130.91038
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32800_Aamu_Bax2006_COI	DRWB-027	1	BE32800_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	GCACAACCGGGATCTTTACTTC	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32800_Pcan_Bot2011_IGS	DRWB-027	1	BE32800_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-027	IGS	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	CGTAATCCTCAGTACTGGCTA	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32800_Pper_Dia2013_COI	DRWB-027	1	BE32800_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	CTTAGTGGCATTAATTCGDAATCC	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32800_Sspa_Oph2007_COI	DRWB-027	1	BE32800_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	GAAGGCCTTCCGATGATC	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32800_Upin_Bot2011_COI	DRWB-027	1	BE32800_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	CTTTAATTACAGCGTTTTTATTGTTGT	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32800_Vgib_Wil2025_COI	DRWB-027	1	BE32800_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	CCCCTCTTTACAAGTTCTTTAGGGT	130.91277
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32801_Asen_Wil2025_COI	DRWB-028	1	BE32801_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	GTTGTTGTTACAACACATGCTCTTTTG	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32801_Cjap_Sim2018_COI	DRWB-028	1	BE32801_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32801_Esin_And2018_Cyb	DRWB-028	1	BE32801_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-028	Cytb	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	ACCCCTCCTCATATCCAACCA	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32801_Mgig_Bot2012_COI	DRWB-028	1	BE32801_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	TCTTATTCGTTGGAGACTTTATAACCCT	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32801_Mjap_Gib2022_COI	DRWB-028	1	BE32801_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	AATTTAATTGTGACTAGGCATGGTCTG	130.91384
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32802_Cmae_Bot2011_COI	DRWB-029	1	BE32802_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	ATGAACAGTCTATCCTCCTTTAG	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32802_Hsan_Wil2021_COI	DRWB-029	1	BE32802_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	CCTGGGCCGGTATAGTAGGT	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32802_Mros_Gib2022_COI	DRWB-029	1	BE32802_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32802_Msal_Bot2012_COI	DRWB-029	1	BE32802_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	GYTAGTTCCRATGATGTTAGCTG	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32802_Mstr_Wil2021_COI	DRWB-029	1	BE32802_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	GTAGTGGTAACTACTCATGCTTTA	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32802_Pvir_Dia2013_COI	DRWB-029	1	BE32802_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	CTTAGTGGCATTAATTCGDAATCC	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32802_Rhar_Sim2018_COI	DRWB-029	1	BE32802_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	CTGCTGCTATTGCTCATG	130.91506
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32804_Aamu_Bax2006_COI	DRWB-031	1	BE32804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	GCACAACCGGGATCTTTACTTC	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32804_Pcan_Bot2011_IGS	DRWB-031	1	BE32804_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-031	IGS	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	CGTAATCCTCAGTACTGGCTA	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32804_Pper_Dia2013_COI	DRWB-031	1	BE32804_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	CTTAGTGGCATTAATTCGDAATCC	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32804_Sspa_Oph2007_COI	DRWB-031	1	BE32804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	GAAGGCCTTCCGATGATC	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32804_Upin_Bot2011_COI	DRWB-031	1	BE32804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	CTTTAATTACAGCGTTTTTATTGTTGT	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32804_Vgib_Wil2025_COI	DRWB-031	1	BE32804_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	CCCCTCTTTACAAGTTCTTTAGGGT	130.90982
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32805_Asen_Wil2025_COI	DRWB-032	1	BE32805_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	GTTGTTGTTACAACACATGCTCTTTTG	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32805_Cjap_Sim2018_COI	DRWB-032	1	BE32805_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32805_Esin_And2018_Cyb	DRWB-032	1	BE32805_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-032	Cytb	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	ACCCCTCCTCATATCCAACCA	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32805_Mgig_Bot2012_COI	DRWB-032	1	BE32805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	TCTTATTCGTTGGAGACTTTATAACCCT	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32805_Mjap_Gib2022_COI	DRWB-032	1	BE32805_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	AATTTAATTGTGACTAGGCATGGTCTG	130.91099
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32806_Cmae_Bot2011_COI	DRWB-033	1	BE32806_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	ATGAACAGTCTATCCTCCTTTAG	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32806_Hsan_Wil2021_COI	DRWB-033	1	BE32806_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	CCTGGGCCGGTATAGTAGGT	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32806_Mros_Gib2022_COI	DRWB-033	1	BE32806_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32806_Msal_Bot2012_COI	DRWB-033	1	BE32806_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	GYTAGTTCCRATGATGTTAGCTG	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32806_Mstr_Wil2021_COI	DRWB-033	1	BE32806_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	GTAGTGGTAACTACTCATGCTTTA	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32806_Pvir_Dia2013_COI	DRWB-033	1	BE32806_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	CTTAGTGGCATTAATTCGDAATCC	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32806_Rhar_Sim2018_COI	DRWB-033	1	BE32806_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	CTGCTGCTATTGCTCATG	130.91223
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32808_Aamu_Bax2006_COI	DRWB-035	1	BE32808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	GCACAACCGGGATCTTTACTTC	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32808_Pcan_Bot2011_IGS	DRWB-035	1	BE32808_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-035	IGS	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	CGTAATCCTCAGTACTGGCTA	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32808_Pper_Dia2013_COI	DRWB-035	1	BE32808_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	CTTAGTGGCATTAATTCGDAATCC	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32808_Sspa_Oph2007_COI	DRWB-035	1	BE32808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	GAAGGCCTTCCGATGATC	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32808_Upin_Bot2011_COI	DRWB-035	1	BE32808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	CTTTAATTACAGCGTTTTTATTGTTGT	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32808_Vgib_Wil2025_COI	DRWB-035	1	BE32808_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	CCCCTCTTTACAAGTTCTTTAGGGT	130.91296
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32815_Aamu_Bax2006_COI	DRWB-036	1	BE32815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	GCACAACCGGGATCTTTACTTC	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32815_Pcan_Bot2011_IGS	DRWB-036	1	BE32815_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-036	IGS	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	CGTAATCCTCAGTACTGGCTA	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32815_Pper_Dia2013_COI	DRWB-036	1	BE32815_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	CTTAGTGGCATTAATTCGDAATCC	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32815_Sspa_Oph2007_COI	DRWB-036	1	BE32815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	GAAGGCCTTCCGATGATC	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32815_Upin_Bot2011_COI	DRWB-036	1	BE32815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	CTTTAATTACAGCGTTTTTATTGTTGT	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32815_Vgib_Wil2025_COI	DRWB-036	1	BE32815_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	CCCCTCTTTACAAGTTCTTTAGGGT	130.84724
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32816_Asen_Wil2025_COI	DRWB-037	1	BE32816_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	GTTGTTGTTACAACACATGCTCTTTTG	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32816_Cjap_Sim2018_COI	DRWB-037	1	BE32816_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32816_Esin_And2018_Cyb	DRWB-037	1	BE32816_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-037	Cytb	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	ACCCCTCCTCATATCCAACCA	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32816_Mgig_Bot2012_COI	DRWB-037	1	BE32816_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	TCTTATTCGTTGGAGACTTTATAACCCT	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32816_Mjap_Gib2022_COI	DRWB-037	1	BE32816_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	AATTTAATTGTGACTAGGCATGGTCTG	130.84861
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32817_Cmae_Bot2011_COI	DRWB-038	1	BE32817_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	ATGAACAGTCTATCCTCCTTTAG	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32817_Hsan_Wil2021_COI	DRWB-038	1	BE32817_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	CCTGGGCCGGTATAGTAGGT	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32817_Mros_Gib2022_COI	DRWB-038	1	BE32817_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32817_Msal_Bot2012_COI	DRWB-038	1	BE32817_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	GYTAGTTCCRATGATGTTAGCTG	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32817_Mstr_Wil2021_COI	DRWB-038	1	BE32817_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	GTAGTGGTAACTACTCATGCTTTA	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32817_Pvir_Dia2013_COI	DRWB-038	1	BE32817_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	CTTAGTGGCATTAATTCGDAATCC	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32817_Rhar_Sim2018_COI	DRWB-038	1	BE32817_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	CTGCTGCTATTGCTCATG	130.84991
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32819_Aamu_Bax2006_COI	DRWB-040	1	BE32819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	GCACAACCGGGATCTTTACTTC	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32819_Pcan_Bot2011_IGS	DRWB-040	1	BE32819_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-040	IGS	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	CGTAATCCTCAGTACTGGCTA	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32819_Pper_Dia2013_COI	DRWB-040	1	BE32819_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	CTTAGTGGCATTAATTCGDAATCC	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32819_Sspa_Oph2007_COI	DRWB-040	1	BE32819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	GAAGGCCTTCCGATGATC	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32819_Upin_Bot2011_COI	DRWB-040	1	BE32819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	CTTTAATTACAGCGTTTTTATTGTTGT	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32819_Vgib_Wil2025_COI	DRWB-040	1	BE32819_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	CCCCTCTTTACAAGTTCTTTAGGGT	130.85255
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32822_Aamu_Bax2006_COI	DRWB-043	1	BE32822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	GCACAACCGGGATCTTTACTTC	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32822_Pcan_Bot2011_IGS	DRWB-043	1	BE32822_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-043	IGS	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	CGTAATCCTCAGTACTGGCTA	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32822_Pper_Dia2013_COI	DRWB-043	1	BE32822_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	CTTAGTGGCATTAATTCGDAATCC	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32822_Sspa_Oph2007_COI	DRWB-043	1	BE32822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	GAAGGCCTTCCGATGATC	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32822_Upin_Bot2011_COI	DRWB-043	1	BE32822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	CTTTAATTACAGCGTTTTTATTGTTGT	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32822_Vgib_Wil2025_COI	DRWB-043	1	BE32822_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	CCCCTCTTTACAAGTTCTTTAGGGT	130.84921
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32823_Asen_Wil2025_COI	DRWB-044	1	BE32823_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	GTTGTTGTTACAACACATGCTCTTTTG	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32823_Cjap_Sim2018_COI	DRWB-044	1	BE32823_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32823_Esin_And2018_Cyb	DRWB-044	1	BE32823_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-044	Cytb	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	ACCCCTCCTCATATCCAACCA	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32823_Mgig_Bot2012_COI	DRWB-044	1	BE32823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	TCTTATTCGTTGGAGACTTTATAACCCT	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32823_Mjap_Gib2022_COI	DRWB-044	1	BE32823_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	AATTTAATTGTGACTAGGCATGGTCTG	130.85045
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32824_Cmae_Bot2011_COI	DRWB-045	1	BE32824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	ATGAACAGTCTATCCTCCTTTAG	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32824_Hsan_Wil2021_COI	DRWB-045	1	BE32824_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	CCTGGGCCGGTATAGTAGGT	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32824_Mros_Gib2022_COI	DRWB-045	1	BE32824_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32824_Msal_Bot2012_COI	DRWB-045	1	BE32824_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	GYTAGTTCCRATGATGTTAGCTG	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32824_Mstr_Wil2021_COI	DRWB-045	1	BE32824_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	GTAGTGGTAACTACTCATGCTTTA	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32824_Pvir_Dia2013_COI	DRWB-045	1	BE32824_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	CTTAGTGGCATTAATTCGDAATCC	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32824_Rhar_Sim2018_COI	DRWB-045	1	BE32824_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	CTGCTGCTATTGCTCATG	130.8519
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32826_Aamu_Bax2006_COI	DRWB-047	1	BE32826_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	GCACAACCGGGATCTTTACTTC	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32826_Pcan_Bot2011_IGS	DRWB-047	1	BE32826_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-047	IGS	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	CGTAATCCTCAGTACTGGCTA	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32826_Pper_Dia2013_COI	DRWB-047	1	BE32826_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	CTTAGTGGCATTAATTCGDAATCC	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32826_Sspa_Oph2007_COI	DRWB-047	1	BE32826_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	GAAGGCCTTCCGATGATC	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32826_Upin_Bot2011_COI	DRWB-047	1	BE32826_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	CTTTAATTACAGCGTTTTTATTGTTGT	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32826_Vgib_Wil2025_COI	DRWB-047	1	BE32826_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	CCCCTCTTTACAAGTTCTTTAGGGT	130.84593
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32827_Asen_Wil2025_COI	DRWB-048	1	BE32827_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	GTTGTTGTTACAACACATGCTCTTTTG	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32827_Cjap_Sim2018_COI	DRWB-048	1	BE32827_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32827_Esin_And2018_Cyb	DRWB-048	1	BE32827_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-048	Cytb	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	ACCCCTCCTCATATCCAACCA	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32827_Mgig_Bot2012_COI	DRWB-048	1	BE32827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	TCTTATTCGTTGGAGACTTTATAACCCT	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32827_Mjap_Gib2022_COI	DRWB-048	1	BE32827_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	AATTTAATTGTGACTAGGCATGGTCTG	130.8473
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32828_Cmae_Bot2011_COI	DRWB-049	1	BE32828_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	ATGAACAGTCTATCCTCCTTTAG	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32828_Hsan_Wil2021_COI	DRWB-049	1	BE32828_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	CCTGGGCCGGTATAGTAGGT	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32828_Mros_Gib2022_COI	DRWB-049	1	BE32828_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32828_Msal_Bot2012_COI	DRWB-049	1	BE32828_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	GYTAGTTCCRATGATGTTAGCTG	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32828_Mstr_Wil2021_COI	DRWB-049	1	BE32828_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	GTAGTGGTAACTACTCATGCTTTA	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32828_Pvir_Dia2013_COI	DRWB-049	1	BE32828_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	CTTAGTGGCATTAATTCGDAATCC	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32828_Rhar_Sim2018_COI	DRWB-049	1	BE32828_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	CTGCTGCTATTGCTCATG	130.84536
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32830_Asen_Wil2025_COI	DRWB-051	1	BE32830_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	GTTGTTGTTACAACACATGCTCTTTTG	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32830_Cjap_Sim2018_COI	DRWB-051	1	BE32830_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32830_Esin_And2018_Cyb	DRWB-051	1	BE32830_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-051	Cytb	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	ACCCCTCCTCATATCCAACCA	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32830_Mgig_Bot2012_COI	DRWB-051	1	BE32830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	TCTTATTCGTTGGAGACTTTATAACCCT	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32830_Mjap_Gib2022_COI	DRWB-051	1	BE32830_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	AATTTAATTGTGACTAGGCATGGTCTG	130.848
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32831_Cmae_Bot2011_COI	DRWB-052	1	BE32831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	ATGAACAGTCTATCCTCCTTTAG	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32831_Hsan_Wil2021_COI	DRWB-052	1	BE32831_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	CCTGGGCCGGTATAGTAGGT	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32831_Mros_Gib2022_COI	DRWB-052	1	BE32831_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32831_Msal_Bot2012_COI	DRWB-052	1	BE32831_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	GYTAGTTCCRATGATGTTAGCTG	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32831_Mstr_Wil2021_COI	DRWB-052	1	BE32831_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	GTAGTGGTAACTACTCATGCTTTA	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32831_Pvir_Dia2013_COI	DRWB-052	1	BE32831_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	CTTAGTGGCATTAATTCGDAATCC	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32831_Rhar_Sim2018_COI	DRWB-052	1	BE32831_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	CTGCTGCTATTGCTCATG	130.85133
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32833_Aamu_Bax2006_COI	DRWB-054	1	BE32833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	GCACAACCGGGATCTTTACTTC	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32833_Pcan_Bot2011_IGS	DRWB-054	1	BE32833_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-054	IGS	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	CGTAATCCTCAGTACTGGCTA	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32833_Pper_Dia2013_COI	DRWB-054	1	BE32833_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	CTTAGTGGCATTAATTCGDAATCC	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32833_Sspa_Oph2007_COI	DRWB-054	1	BE32833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	GAAGGCCTTCCGATGATC	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32833_Upin_Bot2011_COI	DRWB-054	1	BE32833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	CTTTAATTACAGCGTTTTTATTGTTGT	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32833_Vgib_Wil2025_COI	DRWB-054	1	BE32833_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	CCCCTCTTTACAAGTTCTTTAGGGT	130.84601
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32834_Asen_Wil2025_COI	DRWB-055	1	BE32834_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	GTTGTTGTTACAACACATGCTCTTTTG	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32834_Cjap_Sim2018_COI	DRWB-055	1	BE32834_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32834_Esin_And2018_Cyb	DRWB-055	1	BE32834_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-055	Cytb	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	ACCCCTCCTCATATCCAACCA	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32834_Mgig_Bot2012_COI	DRWB-055	1	BE32834_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	TCTTATTCGTTGGAGACTTTATAACCCT	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32834_Mjap_Gib2022_COI	DRWB-055	1	BE32834_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	AATTTAATTGTGACTAGGCATGGTCTG	130.84732
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32835_Cmae_Bot2011_COI	DRWB-056	1	BE32835_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	ATGAACAGTCTATCCTCCTTTAG	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32835_Hsan_Wil2021_COI	DRWB-056	1	BE32835_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	CCTGGGCCGGTATAGTAGGT	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32835_Mros_Gib2022_COI	DRWB-056	1	BE32835_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32835_Msal_Bot2012_COI	DRWB-056	1	BE32835_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	GYTAGTTCCRATGATGTTAGCTG	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32835_Mstr_Wil2021_COI	DRWB-056	1	BE32835_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	GTAGTGGTAACTACTCATGCTTTA	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32835_Pvir_Dia2013_COI	DRWB-056	1	BE32835_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	CTTAGTGGCATTAATTCGDAATCC	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32835_Rhar_Sim2018_COI	DRWB-056	1	BE32835_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	CTGCTGCTATTGCTCATG	130.84535
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32837_Aamu_Bax2006_COI	DRWB-058	1	BE32837_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	GCACAACCGGGATCTTTACTTC	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32837_Pcan_Bot2011_IGS	DRWB-058	1	BE32837_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-058	IGS	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	CGTAATCCTCAGTACTGGCTA	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32837_Pper_Dia2013_COI	DRWB-058	1	BE32837_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	CTTAGTGGCATTAATTCGDAATCC	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32837_Sspa_Oph2007_COI	DRWB-058	1	BE32837_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	GAAGGCCTTCCGATGATC	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32837_Upin_Bot2011_COI	DRWB-058	1	BE32837_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	CTTTAATTACAGCGTTTTTATTGTTGT	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32837_Vgib_Wil2025_COI	DRWB-058	1	BE32837_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	CCCCTCTTTACAAGTTCTTTAGGGT	130.85111
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32838_Asen_Wil2025_COI	DRWB-059	1	BE32838_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	GTTGTTGTTACAACACATGCTCTTTTG	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32838_Cjap_Sim2018_COI	DRWB-059	1	BE32838_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32838_Esin_And2018_Cyb	DRWB-059	1	BE32838_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-059	Cytb	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	ACCCCTCCTCATATCCAACCA	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32838_Mgig_Bot2012_COI	DRWB-059	1	BE32838_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	TCTTATTCGTTGGAGACTTTATAACCCT	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32838_Mjap_Gib2022_COI	DRWB-059	1	BE32838_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	AATTTAATTGTGACTAGGCATGGTCTG	130.84464
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32839_Cmae_Bot2011_COI	DRWB-060	1	BE32839_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	ATGAACAGTCTATCCTCCTTTAG	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32839_Hsan_Wil2021_COI	DRWB-060	1	BE32839_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	CCTGGGCCGGTATAGTAGGT	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32839_Mros_Gib2022_COI	DRWB-060	1	BE32839_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32839_Msal_Bot2012_COI	DRWB-060	1	BE32839_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	GYTAGTTCCRATGATGTTAGCTG	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32839_Mstr_Wil2021_COI	DRWB-060	1	BE32839_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	GTAGTGGTAACTACTCATGCTTTA	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32839_Pvir_Dia2013_COI	DRWB-060	1	BE32839_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	CTTAGTGGCATTAATTCGDAATCC	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32839_Rhar_Sim2018_COI	DRWB-060	1	BE32839_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	CTGCTGCTATTGCTCATG	130.84601
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32840_Aamu_Bax2006_COI	DRWB-061	1	BE32840_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	GCACAACCGGGATCTTTACTTC	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32840_Pcan_Bot2011_IGS	DRWB-061	1	BE32840_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-061	IGS	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	CGTAATCCTCAGTACTGGCTA	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32840_Pper_Dia2013_COI	DRWB-061	1	BE32840_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	CTTAGTGGCATTAATTCGDAATCC	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32840_Sspa_Oph2007_COI	DRWB-061	1	BE32840_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	GAAGGCCTTCCGATGATC	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32840_Upin_Bot2011_COI	DRWB-061	1	BE32840_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	CTTTAATTACAGCGTTTTTATTGTTGT	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32840_Vgib_Wil2025_COI	DRWB-061	1	BE32840_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	CCCCTCTTTACAAGTTCTTTAGGGT	130.84739
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32841_Asen_Wil2025_COI	DRWB-062	1	BE32841_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	GTTGTTGTTACAACACATGCTCTTTTG	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32841_Cjap_Sim2018_COI	DRWB-062	1	BE32841_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32841_Esin_And2018_Cyb	DRWB-062	1	BE32841_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-062	Cytb	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	ACCCCTCCTCATATCCAACCA	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32841_Mgig_Bot2012_COI	DRWB-062	1	BE32841_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	TCTTATTCGTTGGAGACTTTATAACCCT	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32841_Mjap_Gib2022_COI	DRWB-062	1	BE32841_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	AATTTAATTGTGACTAGGCATGGTCTG	130.84871
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32842_Cmae_Bot2011_COI	DRWB-063	1	BE32842_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	ATGAACAGTCTATCCTCCTTTAG	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32842_Hsan_Wil2021_COI	DRWB-063	1	BE32842_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	CCTGGGCCGGTATAGTAGGT	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32842_Mros_Gib2022_COI	DRWB-063	1	BE32842_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32842_Msal_Bot2012_COI	DRWB-063	1	BE32842_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	GYTAGTTCCRATGATGTTAGCTG	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32842_Mstr_Wil2021_COI	DRWB-063	1	BE32842_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	GTAGTGGTAACTACTCATGCTTTA	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32842_Pvir_Dia2013_COI	DRWB-063	1	BE32842_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	CTTAGTGGCATTAATTCGDAATCC	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32842_Rhar_Sim2018_COI	DRWB-063	1	BE32842_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	CTGCTGCTATTGCTCATG	130.85008
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32844_Aamu_Bax2006_COI	DRWB-065	1	BE32844_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	GCACAACCGGGATCTTTACTTC	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32844_Pcan_Bot2011_IGS	DRWB-065	1	BE32844_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-065	IGS	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	CGTAATCCTCAGTACTGGCTA	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32844_Pper_Dia2013_COI	DRWB-065	1	BE32844_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	CTTAGTGGCATTAATTCGDAATCC	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32844_Sspa_Oph2007_COI	DRWB-065	1	BE32844_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	GAAGGCCTTCCGATGATC	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32844_Upin_Bot2011_COI	DRWB-065	1	BE32844_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	CTTTAATTACAGCGTTTTTATTGTTGT	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32844_Vgib_Wil2025_COI	DRWB-065	1	BE32844_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	CCCCTCTTTACAAGTTCTTTAGGGT	130.84676
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32845_Asen_Wil2025_COI	DRWB-066	1	BE32845_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	GTTGTTGTTACAACACATGCTCTTTTG	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32845_Cjap_Sim2018_COI	DRWB-066	1	BE32845_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32845_Esin_And2018_Cyb	DRWB-066	1	BE32845_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-066	Cytb	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	ACCCCTCCTCATATCCAACCA	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32845_Mgig_Bot2012_COI	DRWB-066	1	BE32845_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	TCTTATTCGTTGGAGACTTTATAACCCT	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32845_Mjap_Gib2022_COI	DRWB-066	1	BE32845_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	AATTTAATTGTGACTAGGCATGGTCTG	130.8481
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32846_Cmae_Bot2011_COI	DRWB-067	1	BE32846_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	ATGAACAGTCTATCCTCCTTTAG	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32846_Hsan_Wil2021_COI	DRWB-067	1	BE32846_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	CCTGGGCCGGTATAGTAGGT	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32846_Mros_Gib2022_COI	DRWB-067	1	BE32846_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32846_Msal_Bot2012_COI	DRWB-067	1	BE32846_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	GYTAGTTCCRATGATGTTAGCTG	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32846_Mstr_Wil2021_COI	DRWB-067	1	BE32846_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	GTAGTGGTAACTACTCATGCTTTA	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32846_Pvir_Dia2013_COI	DRWB-067	1	BE32846_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	CTTAGTGGCATTAATTCGDAATCC	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32846_Rhar_Sim2018_COI	DRWB-067	1	BE32846_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	CTGCTGCTATTGCTCATG	130.84942
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32848_Aamu_Bax2006_COI	DRWB-069	1	BE32848_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	GCACAACCGGGATCTTTACTTC	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32848_Pcan_Bot2011_IGS	DRWB-069	1	BE32848_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-069	IGS	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	CGTAATCCTCAGTACTGGCTA	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32848_Pper_Dia2013_COI	DRWB-069	1	BE32848_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	CTTAGTGGCATTAATTCGDAATCC	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32848_Sspa_Oph2007_COI	DRWB-069	1	BE32848_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	GAAGGCCTTCCGATGATC	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32848_Upin_Bot2011_COI	DRWB-069	1	BE32848_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	CTTTAATTACAGCGTTTTTATTGTTGT	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32848_Vgib_Wil2025_COI	DRWB-069	1	BE32848_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	CCCCTCTTTACAAGTTCTTTAGGGT	130.84876
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32849_Asen_Wil2025_COI	DRWB-070	1	BE32849_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	GTTGTTGTTACAACACATGCTCTTTTG	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32849_Cjap_Sim2018_COI	DRWB-070	1	BE32849_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32849_Esin_And2018_Cyb	DRWB-070	1	BE32849_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-070	Cytb	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	ACCCCTCCTCATATCCAACCA	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32849_Mgig_Bot2012_COI	DRWB-070	1	BE32849_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	TCTTATTCGTTGGAGACTTTATAACCCT	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32849_Mjap_Gib2022_COI	DRWB-070	1	BE32849_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	AATTTAATTGTGACTAGGCATGGTCTG	130.85013
id	detectionCriteria	pcrPlateID	decimalLatitude	geodeticDatum	materialSampleID	pcrProbeSeq	pcrProbeRepoter	ampVisMethod	basisOfRecord	quantificationCycle	coordinateUncertaintyInMetres	targetTaxonomicAssay	sampleMaterialProcess	pcrInhibitionScaleFactor	pcrProbeQuencher	associatedReferences	parentEventID	eventDate	pcrPrimerREV	biome	scientificName	pcrEfficiency	pcrAmpliconSize	catalogueNumber	sourceMaterialID	pcrInhibitionCheck01	occurrenceID	samplingProtocol	sampleCollectionMethod	eventID	eventTypeHierarchy	sampleName	assayType	occurrenceStatus	eventType	assayName	materialEntityID	pcrTargetGene	pcrCycles	eventHierarchy	coordinatePrecision	pcrInhibitionCheck	country	fieldNumber	pcrPrimerFWD	decimalLongitude
BA83935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05434	-34.80968	WGS84	BA83935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.591	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83935_Cmae_Bot2011_COI	A61	1	BA83935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83935	Sample	BA83935	targeted	Present	Sample	Cmae_Bot2011_COI	A61	COI	45	BA83935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A61	ATGAACAGTCTATCCTCCTTTAG	138.52004
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83962_Aamu_Bax2006_COI	C4	1	BA83962_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Aamu_Bax2006_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	GCACAACCGGGATCTTTACTTC	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83962_Pcan_Bot2011_IGS	C4	1	BA83962_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Pcan_Bot2011_IGS	C4	IGS	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	CGTAATCCTCAGTACTGGCTA	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83962_Pper_Dia2013_COI	C4	1	BA83962_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Pper_Dia2013_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	CTTAGTGGCATTAATTCGDAATCC	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83962_Sspa_Oph2007_COI	C4	1	BA83962_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Sspa_Oph2007_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	GAAGGCCTTCCGATGATC	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83962_Upin_Bot2011_COI	C4	1	BA83962_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Upin_Bot2011_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	CTTTAATTACAGCGTTTTTATTGTTGT	145.77379
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83963_Cjap_Sim2018_COI	C5	1	BA83963_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Cjap_Sim2018_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83963_Esin_And2018_Cyb	C5	1	BA83963_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Esin_And2018_Cyb	C5	Cytb	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	ACCCCTCCTCATATCCAACCA	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83963_Mgig_Bot2012_COI	C5	1	BA83963_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Mgig_Bot2012_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	TCTTATTCGTTGGAGACTTTATAACCCT	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA83963_Mjap_Gib2022_COI	C5	1	BA83963_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Mjap_Gib2022_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	AATTTAATTGTGACTAGGCATGGTCTG	145.79636
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83964_Cmae_Bot2011_COI	C6	1	BA83964_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Cmae_Bot2011_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	ATGAACAGTCTATCCTCCTTTAG	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83964_Hsan_Wil2021_COI	C6	1	BA83964_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Hsan_Wil2021_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	CCTGGGCCGGTATAGTAGGT	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83964_Mros_Gib2022_COI	C6	1	BA83964_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Mros_Gib2022_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83964_Msal_Bot2012_COI	C6	1	BA83964_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Msal_Bot2012_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	GYTAGTTCCRATGATGTTAGCTG	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83964_Pvir_Dia2013_COI	C6	1	BA83964_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Pvir_Dia2013_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	CTTAGTGGCATTAATTCGDAATCC	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83964_Rhar_Sim2018_COI	C6	1	BA83964_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Rhar_Sim2018_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	CTGCTGCTATTGCTCATG	145.7847
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83966_Aamu_Bax2006_COI	C11	1	BA83966_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Aamu_Bax2006_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	GCACAACCGGGATCTTTACTTC	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83966_Pcan_Bot2011_IGS	C11	1	BA83966_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Pcan_Bot2011_IGS	C11	IGS	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	CGTAATCCTCAGTACTGGCTA	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83966_Pper_Dia2013_COI	C11	1	BA83966_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Pper_Dia2013_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	CTTAGTGGCATTAATTCGDAATCC	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83966_Sspa_Oph2007_COI	C11	1	BA83966_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Sspa_Oph2007_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	GAAGGCCTTCCGATGATC	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83966_Upin_Bot2011_COI	C11	1	BA83966_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Upin_Bot2011_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	CTTTAATTACAGCGTTTTTATTGTTGT	145.7845
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83967_Cjap_Sim2018_COI	C12	1	BA83967_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Cjap_Sim2018_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83967_Esin_And2018_Cyb	C12	1	BA83967_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Esin_And2018_Cyb	C12	Cytb	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	ACCCCTCCTCATATCCAACCA	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83967_Mgig_Bot2012_COI	C12	1	BA83967_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Mgig_Bot2012_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	TCTTATTCGTTGGAGACTTTATAACCCT	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA83967_Mjap_Gib2022_COI	C12	1	BA83967_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Mjap_Gib2022_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	AATTTAATTGTGACTAGGCATGGTCTG	145.78334
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83968_Cmae_Bot2011_COI	C16	1	BA83968_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Cmae_Bot2011_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	ATGAACAGTCTATCCTCCTTTAG	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83968_Hsan_Wil2021_COI	C16	1	BA83968_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Hsan_Wil2021_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	CCTGGGCCGGTATAGTAGGT	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83968_Mros_Gib2022_COI	C16	1	BA83968_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Mros_Gib2022_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83968_Msal_Bot2012_COI	C16	1	BA83968_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Msal_Bot2012_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	GYTAGTTCCRATGATGTTAGCTG	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83968_Pvir_Dia2013_COI	C16	1	BA83968_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Pvir_Dia2013_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	CTTAGTGGCATTAATTCGDAATCC	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83968_Rhar_Sim2018_COI	C16	1	BA83968_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Rhar_Sim2018_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	CTGCTGCTATTGCTCATG	145.78604
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83970_Cjap_Sim2018_COI	C18	1	BA83970_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Cjap_Sim2018_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83970_Esin_And2018_Cyb	C18	1	BA83970_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Esin_And2018_Cyb	C18	Cytb	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	ACCCCTCCTCATATCCAACCA	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83970_Mgig_Bot2012_COI	C18	1	BA83970_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Mgig_Bot2012_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	TCTTATTCGTTGGAGACTTTATAACCCT	145.78312
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83971_Cmae_Bot2011_COI	C22	1	BA83971_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Cmae_Bot2011_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	ATGAACAGTCTATCCTCCTTTAG	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83971_Hsan_Wil2021_COI	C22	1	BA83971_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Hsan_Wil2021_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	CCTGGGCCGGTATAGTAGGT	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83971_Mros_Gib2022_COI	C22	1	BA83971_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Mros_Gib2022_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83971_Msal_Bot2012_COI	C22	1	BA83971_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Msal_Bot2012_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	GYTAGTTCCRATGATGTTAGCTG	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83971_Pvir_Dia2013_COI	C22	1	BA83971_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Pvir_Dia2013_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	CTTAGTGGCATTAATTCGDAATCC	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83971_Rhar_Sim2018_COI	C22	1	BA83971_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Rhar_Sim2018_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	CTGCTGCTATTGCTCATG	145.78376
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83973_Aamu_Bax2006_COI	C24	1	BA83973_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Aamu_Bax2006_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	GCACAACCGGGATCTTTACTTC	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83973_Pcan_Bot2011_IGS	C24	1	BA83973_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Pcan_Bot2011_IGS	C24	IGS	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	CGTAATCCTCAGTACTGGCTA	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83973_Pper_Dia2013_COI	C24	1	BA83973_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Pper_Dia2013_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	CTTAGTGGCATTAATTCGDAATCC	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83973_Sspa_Oph2007_COI	C24	1	BA83973_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Sspa_Oph2007_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	GAAGGCCTTCCGATGATC	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83973_Upin_Bot2011_COI	C24	1	BA83973_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Upin_Bot2011_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	CTTTAATTACAGCGTTTTTATTGTTGT	145.78334
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83974_Cjap_Sim2018_COI	C28	1	BA83974_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Cjap_Sim2018_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83974_Esin_And2018_Cyb	C28	1	BA83974_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Esin_And2018_Cyb	C28	Cytb	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	ACCCCTCCTCATATCCAACCA	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83974_Mgig_Bot2012_COI	C28	1	BA83974_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Mgig_Bot2012_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	TCTTATTCGTTGGAGACTTTATAACCCT	145.77386
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83975_Cmae_Bot2011_COI	C29	1	BA83975_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Cmae_Bot2011_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	ATGAACAGTCTATCCTCCTTTAG	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83975_Hsan_Wil2021_COI	C29	1	BA83975_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Hsan_Wil2021_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	CCTGGGCCGGTATAGTAGGT	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83975_Mros_Gib2022_COI	C29	1	BA83975_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Mros_Gib2022_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83975_Msal_Bot2012_COI	C29	1	BA83975_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Msal_Bot2012_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	GYTAGTTCCRATGATGTTAGCTG	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83975_Pvir_Dia2013_COI	C29	1	BA83975_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Pvir_Dia2013_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	CTTAGTGGCATTAATTCGDAATCC	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83975_Rhar_Sim2018_COI	C29	1	BA83975_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Rhar_Sim2018_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	CTGCTGCTATTGCTCATG	145.78312
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83977_Aamu_Bax2006_COI	A85	1	BA83977_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Aamu_Bax2006_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	GCACAACCGGGATCTTTACTTC	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83977_Pcan_Bot2011_IGS	A85	1	BA83977_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Pcan_Bot2011_IGS	A85	IGS	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	CGTAATCCTCAGTACTGGCTA	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83977_Pper_Dia2013_COI	A85	1	BA83977_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Pper_Dia2013_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	CTTAGTGGCATTAATTCGDAATCC	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83977_Sspa_Oph2007_COI	A85	1	BA83977_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Sspa_Oph2007_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	GAAGGCCTTCCGATGATC	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83977_Upin_Bot2011_COI	A85	1	BA83977_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Upin_Bot2011_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	CTTTAATTACAGCGTTTTTATTGTTGT	138.52559
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83978_Cjap_Sim2018_COI	A86	1	BA83978_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Cjap_Sim2018_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83978_Esin_And2018_Cyb	A86	1	BA83978_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Esin_And2018_Cyb	A86	Cytb	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	ACCCCTCCTCATATCCAACCA	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83978_Mgig_Bot2012_COI	A86	1	BA83978_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Mgig_Bot2012_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	TCTTATTCGTTGGAGACTTTATAACCCT	138.5217
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.595	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83979_Cmae_Bot2011_COI	A87	1	BA83979_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Present	Sample	Cmae_Bot2011_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	ATGAACAGTCTATCCTCCTTTAG	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83979_Hsan_Wil2021_COI	A87	1	BA83979_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Hsan_Wil2021_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	CCTGGGCCGGTATAGTAGGT	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83979_Mros_Gib2022_COI	A87	1	BA83979_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Mros_Gib2022_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83979_Msal_Bot2012_COI	A87	1	BA83979_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Msal_Bot2012_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	GYTAGTTCCRATGATGTTAGCTG	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83979_Pvir_Dia2013_COI	A87	1	BA83979_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Pvir_Dia2013_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	CTTAGTGGCATTAATTCGDAATCC	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83979_Rhar_Sim2018_COI	A87	1	BA83979_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Rhar_Sim2018_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	CTGCTGCTATTGCTCATG	138.51686
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83980_Aamu_Bax2006_COI	A88	1	BA83980_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Aamu_Bax2006_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	GCACAACCGGGATCTTTACTTC	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83980_Pcan_Bot2011_IGS	A88	1	BA83980_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Pcan_Bot2011_IGS	A88	IGS	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	CGTAATCCTCAGTACTGGCTA	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83980_Pper_Dia2013_COI	A88	1	BA83980_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Pper_Dia2013_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	CTTAGTGGCATTAATTCGDAATCC	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83980_Sspa_Oph2007_COI	A88	1	BA83980_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Sspa_Oph2007_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	GAAGGCCTTCCGATGATC	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83980_Upin_Bot2011_COI	A88	1	BA83980_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Upin_Bot2011_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	CTTTAATTACAGCGTTTTTATTGTTGT	138.51964
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83981_Cjap_Sim2018_COI	A89	1	BA83981_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Cjap_Sim2018_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83981_Esin_And2018_Cyb	A89	1	BA83981_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Esin_And2018_Cyb	A89	Cytb	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	ACCCCTCCTCATATCCAACCA	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83981_Mgig_Bot2012_COI	A89	1	BA83981_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Mgig_Bot2012_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	TCTTATTCGTTGGAGACTTTATAACCCT	138.52362
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83982_Cmae_Bot2011_COI	A90	1	BA83982_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Cmae_Bot2011_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	ATGAACAGTCTATCCTCCTTTAG	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83982_Hsan_Wil2021_COI	A90	1	BA83982_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Hsan_Wil2021_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	CCTGGGCCGGTATAGTAGGT	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83982_Mros_Gib2022_COI	A90	1	BA83982_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Mros_Gib2022_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83982_Msal_Bot2012_COI	A90	1	BA83982_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Msal_Bot2012_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	GYTAGTTCCRATGATGTTAGCTG	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83982_Pvir_Dia2013_COI	A90	1	BA83982_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Pvir_Dia2013_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	CTTAGTGGCATTAATTCGDAATCC	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83982_Rhar_Sim2018_COI	A90	1	BA83982_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Rhar_Sim2018_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	CTGCTGCTATTGCTCATG	138.51743
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83984_Aamu_Bax2006_COI	A92	1	BA83984_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Aamu_Bax2006_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	GCACAACCGGGATCTTTACTTC	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83984_Pcan_Bot2011_IGS	A92	1	BA83984_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Pcan_Bot2011_IGS	A92	IGS	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	CGTAATCCTCAGTACTGGCTA	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83984_Pper_Dia2013_COI	A92	1	BA83984_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Pper_Dia2013_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	CTTAGTGGCATTAATTCGDAATCC	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83984_Sspa_Oph2007_COI	A92	1	BA83984_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Sspa_Oph2007_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	GAAGGCCTTCCGATGATC	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83984_Upin_Bot2011_COI	A92	1	BA83984_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Upin_Bot2011_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	CTTTAATTACAGCGTTTTTATTGTTGT	138.51769
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83985_Cjap_Sim2018_COI	A93	1	BA83985_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Cjap_Sim2018_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83985_Esin_And2018_Cyb	A93	1	BA83985_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Esin_And2018_Cyb	A93	Cytb	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	ACCCCTCCTCATATCCAACCA	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83985_Mgig_Bot2012_COI	A93	1	BA83985_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Mgig_Bot2012_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	TCTTATTCGTTGGAGACTTTATAACCCT	138.52075
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.45	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83986_Cmae_Bot2011_COI	A94	1	BA83986_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Present	Sample	Cmae_Bot2011_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	ATGAACAGTCTATCCTCCTTTAG	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83986_Hsan_Wil2021_COI	A94	1	BA83986_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Hsan_Wil2021_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	CCTGGGCCGGTATAGTAGGT	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83986_Mros_Gib2022_COI	A94	1	BA83986_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Mros_Gib2022_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83986_Msal_Bot2012_COI	A94	1	BA83986_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Msal_Bot2012_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	GYTAGTTCCRATGATGTTAGCTG	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83986_Pvir_Dia2013_COI	A94	1	BA83986_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Pvir_Dia2013_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	CTTAGTGGCATTAATTCGDAATCC	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83986_Rhar_Sim2018_COI	A94	1	BA83986_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Rhar_Sim2018_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	CTGCTGCTATTGCTCATG	138.52268
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83988_Aamu_Bax2006_COI	A96	1	BA83988_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Aamu_Bax2006_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	GCACAACCGGGATCTTTACTTC	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83988_Pcan_Bot2011_IGS	A96	1	BA83988_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Pcan_Bot2011_IGS	A96	IGS	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	CGTAATCCTCAGTACTGGCTA	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83988_Pper_Dia2013_COI	A96	1	BA83988_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Pper_Dia2013_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	CTTAGTGGCATTAATTCGDAATCC	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83988_Sspa_Oph2007_COI	A96	1	BA83988_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Sspa_Oph2007_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	GAAGGCCTTCCGATGATC	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83988_Upin_Bot2011_COI	A96	1	BA83988_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Upin_Bot2011_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	CTTTAATTACAGCGTTTTTATTGTTGT	138.51953
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA87563_Cjap_Sim2018_COI	D4	1	BA87563_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Cjap_Sim2018_COI	D4	COI	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84495
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA87563_Esin_And2018_Cyb	D4	1	BA87563_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Esin_And2018_Cyb	D4	Cytb	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	ACCCCTCCTCATATCCAACCA	130.84495
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA87563_Mjap_Gib2022_COI	D4	1	BA87563_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Mjap_Gib2022_COI	D4	COI	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	AATTTAATTGTGACTAGGCATGGTCTG	130.84495
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA87564_Hsan_Wil2021_COI	D5	1	BA87564_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Hsan_Wil2021_COI	D5	COI	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	CCTGGGCCGGTATAGTAGGT	130.85486
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA87564_Mros_Gib2022_COI	D5	1	BA87564_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Mros_Gib2022_COI	D5	COI	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85486
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA87564_Pvir_Dia2013_COI	D5	1	BA87564_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Pvir_Dia2013_COI	D5	COI	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	CTTAGTGGCATTAATTCGDAATCC	130.85486
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA87564_Rhar_Sim2018_COI	D5	1	BA87564_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Rhar_Sim2018_COI	D5	COI	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	CTGCTGCTATTGCTCATG	130.85486
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA87566_Pper_Dia2013_COI	D10	1	BA87566_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Pper_Dia2013_COI	D10	COI	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	CTTAGTGGCATTAATTCGDAATCC	130.87402
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA87567_Cjap_Sim2018_COI	D11	1	BA87567_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Cjap_Sim2018_COI	D11	COI	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87453
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA87567_Esin_And2018_Cyb	D11	1	BA87567_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Esin_And2018_Cyb	D11	Cytb	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	ACCCCTCCTCATATCCAACCA	130.87453
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA87567_Mjap_Gib2022_COI	D11	1	BA87567_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Mjap_Gib2022_COI	D11	COI	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	AATTTAATTGTGACTAGGCATGGTCTG	130.87453
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA87568_Hsan_Wil2021_COI	D12	1	BA87568_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Hsan_Wil2021_COI	D12	COI	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	CCTGGGCCGGTATAGTAGGT	130.8249
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA87568_Mros_Gib2022_COI	D12	1	BA87568_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Mros_Gib2022_COI	D12	COI	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.8249
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA87568_Pvir_Dia2013_COI	D12	1	BA87568_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Pvir_Dia2013_COI	D12	COI	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	CTTAGTGGCATTAATTCGDAATCC	130.8249
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA87568_Rhar_Sim2018_COI	D12	1	BA87568_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Rhar_Sim2018_COI	D12	COI	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	CTGCTGCTATTGCTCATG	130.8249
BA92898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-42.88402	WGS84	BA92898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.706	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_1	29/05/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA92898_Aamu_Bax2006_COI	H4	1	BA92898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92898	Sample	BA92898	targeted	Present	Sample	Aamu_Bax2006_COI	H4	COI	45	BA92898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H4	GCACAACCGGGATCTTTACTTC	147.33767
BA92908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77803	WGS84	BA92908	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.189	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92908_Cmae_Bot2011_COI	NP14	1	BA92908_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92908	Sample	BA92908	targeted	Present	Sample	Cmae_Bot2011_COI	NP14	COI	45	BA92908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP14	ATGAACAGTCTATCCTCCTTTAG	138.48172
BA92911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77537	WGS84	BA92911	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.106	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92911_Cmae_Bot2011_COI	NP13	1	BA92911_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92911	Sample	BA92911	targeted	Present	Sample	Cmae_Bot2011_COI	NP13	COI	45	BA92911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP13	ATGAACAGTCTATCCTCCTTTAG	138.48363
BA92931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77872	WGS84	BA92931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.66623306	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92931_Sspa_Oph2007_COI	NP12	1	BA92931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92931	Sample	BA92931	targeted	Present	Sample	Sspa_Oph2007_COI	NP12	COI	45	BA92931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP12	GAAGGCCTTCCGATGATC	138.48107
BA92935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77575	WGS84	BA92935	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.59156036	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92935_Sspa_Oph2007_COI	NP7	1	BA92935_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92935	Sample	BA92935	targeted	Present	Sample	Sspa_Oph2007_COI	NP7	COI	45	BA92935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP7	GAAGGCCTTCCGATGATC	138.48302
BA92942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77755	WGS84	BA92942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.3269043	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92942_Sspa_Oph2007_COI	NP16	1	BA92942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92942	Sample	BA92942	targeted	Present	Sample	Sspa_Oph2007_COI	NP16	COI	45	BA92942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP16	GAAGGCCTTCCGATGATC	138.48188
BA99028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.81942	WGS84	BA99028	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.19	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99028_Aamu_Bax2006_COI	M16	1	BA99028_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99028	Sample	BA99028	targeted	Present	Sample	Aamu_Bax2006_COI	M16	COI	45	BA99028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M16	GCACAACCGGGATCTTTACTTC	144.91638
BA99031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84247	WGS84	BA99031	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.994	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99031_Aamu_Bax2006_COI	M28	1	BA99031_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99031	Sample	BA99031	targeted	Present	Sample	Aamu_Bax2006_COI	M28	COI	45	BA99031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M28	GCACAACCGGGATCTTTACTTC	144.91785
BA99033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.82031	WGS84	BA99033	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.03	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA99033_Cmae_Bot2011_COI	M30	1	BA99033_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99033	Sample	BA99033	targeted	Present	Sample	Cmae_Bot2011_COI	M30	COI	45	BA99033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M30	ATGAACAGTCTATCCTCCTTTAG	144.90882
BA99035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84518	WGS84	BA99035	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.35	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99035_Aamu_Bax2006_COI	M5	1	BA99035_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99035	Sample	BA99035	targeted	Present	Sample	Aamu_Bax2006_COI	M5	COI	45	BA99035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M5	GCACAACCGGGATCTTTACTTC	144.9296
BA99035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84518	WGS84	BA99035	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.40000153	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA99035_Sspa_Oph2007_COI	M5	1	BA99035_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99035	Sample	BA99035	targeted	Present	Sample	Sspa_Oph2007_COI	M5	COI	45	BA99035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M5	GAAGGCCTTCCGATGATC	144.9296
BA99035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84518	WGS84	BA99035	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.498	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA99035_Upin_Bot2011_COI	M5	1	BA99035_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99035	Sample	BA99035	targeted	Present	Sample	Upin_Bot2011_COI	M5	COI	45	BA99035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M5	CTTTAATTACAGCGTTTTTATTGTTGT	144.9296
BA99039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.82555	WGS84	BA99039	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.613	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99039_Aamu_Bax2006_COI	M12	1	BA99039_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99039	Sample	BA99039	targeted	Present	Sample	Aamu_Bax2006_COI	M12	COI	45	BA99039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M12	GCACAACCGGGATCTTTACTTC	144.90092
BA99042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84168	WGS84	BA99042	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.973	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99042_Aamu_Bax2006_COI	M24	1	BA99042_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99042	Sample	BA99042	targeted	Present	Sample	Aamu_Bax2006_COI	M24	COI	45	BA99042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M24	GCACAACCGGGATCTTTACTTC	144.92731
BB10183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.82639	WGS84	BB10183	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.311	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10183_Cmae_Bot2011_COI	AS2	1	BB10183_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10183	Sample	BB10183	targeted	Present	Sample	Cmae_Bot2011_COI	AS2	COI	45	BB10183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS2	ATGAACAGTCTATCCTCCTTTAG	138.50741
BB10187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81921	WGS84	BB10187	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.924	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10187_Cmae_Bot2011_COI	AS6	1	BB10187_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10187	Sample	BB10187	targeted	Present	Sample	Cmae_Bot2011_COI	AS6	COI	45	BB10187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS6	ATGAACAGTCTATCCTCCTTTAG	138.5112
BB10194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81166	WGS84	BB10194	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.263	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10194_Cmae_Bot2011_COI	AS13	1	BB10194_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10194	Sample	BB10194	targeted	Present	Sample	Cmae_Bot2011_COI	AS13	COI	45	BB10194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS13	ATGAACAGTCTATCCTCCTTTAG	138.51389
BB10202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.82108	WGS84	BB10202	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.116	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10202_Cmae_Bot2011_COI	AS21	1	BB10202_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10202	Sample	BB10202	targeted	Present	Sample	Cmae_Bot2011_COI	AS21	COI	45	BB10202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS21	ATGAACAGTCTATCCTCCTTTAG	138.51044
BB12679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88398	WGS84	BB12679	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.209	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB12679_Aamu_Bax2006_COI	H37	1	BB12679_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12679	Sample	BB12679	targeted	Present	Sample	Aamu_Bax2006_COI	H37	COI	45	BB12679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H37	GCACAACCGGGATCTTTACTTC	147.33726
BB12683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87437	WGS84	BB12683	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.445	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12683_Mgig_Bot2012_COI	H41	1	BB12683_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12683	Sample	BB12683	targeted	Present	Sample	Mgig_Bot2012_COI	H41	COI	45	BB12683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H41	TCTTATTCGTTGGAGACTTTATAACCCT	147.33773
BB12686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88485	WGS84	BB12686	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.946	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB12686_Aamu_Bax2006_COI	H44	1	BB12686_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12686	Sample	BB12686	targeted	Present	Sample	Aamu_Bax2006_COI	H44	COI	45	BB12686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H44	GCACAACCGGGATCTTTACTTC	147.33488
BB12687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88542	WGS84	BB12687	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	21.421	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12687_Mgig_Bot2012_COI	H45	1	BB12687_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12687	Sample	BB12687	targeted	Present	Sample	Mgig_Bot2012_COI	H45	COI	45	BB12687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H45	TCTTATTCGTTGGAGACTTTATAACCCT	147.33407
BB12688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88252	WGS84	BB12688	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.722	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12688_Cmae_Bot2011_COI	H46	1	BB12688_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12688	Sample	BB12688	targeted	Present	Sample	Cmae_Bot2011_COI	H46	COI	45	BB12688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H46	ATGAACAGTCTATCCTCCTTTAG	147.34067
BB12690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.83967	WGS84	BB12690	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.537	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12690_Mgig_Bot2012_COI	H48	1	BB12690_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12690	Sample	BB12690	targeted	Present	Sample	Mgig_Bot2012_COI	H48	COI	45	BB12690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H48	TCTTATTCGTTGGAGACTTTATAACCCT	147.34096
BB12694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87855	WGS84	BB12694	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.023	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12694_Mgig_Bot2012_COI	H52	1	BB12694_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12694	Sample	BB12694	targeted	Present	Sample	Mgig_Bot2012_COI	H52	COI	45	BB12694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H52	TCTTATTCGTTGGAGACTTTATAACCCT	147.34145
BB12695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88468	WGS84	BB12695	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.194	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12695_Cmae_Bot2011_COI	H53	1	BB12695_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12695	Sample	BB12695	targeted	Present	Sample	Cmae_Bot2011_COI	H53	COI	45	BB12695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H53	ATGAACAGTCTATCCTCCTTTAG	147.33773
BB16126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.82865	WGS84	BB16126	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.974	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB16126_Mgig_Bot2012_COI	AS34	1	BB16126_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16126	Sample	BB16126	targeted	Present	Sample	Mgig_Bot2012_COI	AS34	COI	45	BB16126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS34	TCTTATTCGTTGGAGACTTTATAACCCT	138.5089
BB16132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.77087	WGS84	BB16132	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB16132_Aamu_Bax2006_COI	AS40	1	BB16132_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16132	Sample	BB16132	targeted	Absent	Sample	Aamu_Bax2006_COI	AS40	COI	45	BB16132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS40	GCACAACCGGGATCTTTACTTC	138.48653
BB16136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.77223	WGS84	BB16136	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.00400162	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB16136_Sspa_Oph2007_COI	AS44	1	BB16136_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16136	Sample	BB16136	targeted	Present	Sample	Sspa_Oph2007_COI	AS44	COI	45	BB16136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS44	GAAGGCCTTCCGATGATC	138.48573
BB16138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.82417	WGS84	BB16138	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.035	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB16138_Cmae_Bot2011_COI	AS46	1	BB16138_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16138	Sample	BB16138	targeted	Present	Sample	Cmae_Bot2011_COI	AS46	COI	45	BB16138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS46	ATGAACAGTCTATCCTCCTTTAG	138.51043
BB17783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84798	WGS84	BB17783	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.592	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17783_Mgig_Bot2012_COI	S40	1	BB17783_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17783	Sample	BB17783	targeted	Present	Sample	Mgig_Bot2012_COI	S40	COI	45	BB17783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S40	TCTTATTCGTTGGAGACTTTATAACCCT	151.27465
BB17787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84523	WGS84	BB17787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.955	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17787_Mgig_Bot2012_COI	S44	1	BB17787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17787	Sample	BB17787	targeted	Present	Sample	Mgig_Bot2012_COI	S44	COI	45	BB17787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S44	TCTTATTCGTTGGAGACTTTATAACCCT	151.27742
BB17790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84507	WGS84	BB17790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.324	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17790_Mgig_Bot2012_COI	S47	1	BB17790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17790	Sample	BB17790	targeted	Present	Sample	Mgig_Bot2012_COI	S47	COI	45	BB17790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S47	TCTTATTCGTTGGAGACTTTATAACCCT	151.27762
BB17794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84064	WGS84	BB17794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.262	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17794_Mgig_Bot2012_COI	S51	1	BB17794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17794	Sample	BB17794	targeted	Present	Sample	Mgig_Bot2012_COI	S51	COI	45	BB17794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S51	TCTTATTCGTTGGAGACTTTATAACCCT	151.25429
BB32902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01817	WGS84	BB32902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32902_Mgig_Bot2012_COI	PS-A01	1	BB32902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32902	Sample	BB32902	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A01	COI	45	BB32902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A01	TCTTATTCGTTGGAGACTTTATAACCCT	137.60078
BB32903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01742	WGS84	BB32903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32903_Cmae_Bot2011_COI	PS-A02	1	BB32903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32903	Sample	BB32903	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A02	COI	45	BB32903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A02	ATGAACAGTCTATCCTCCTTTAG	137.59663
BB32903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01742	WGS84	BB32903	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32903_Msal_Bot2012_COI	PS-A02	1	BB32903_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32903	Sample	BB32903	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A02	COI	45	BB32903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A02	GYTAGTTCCRATGATGTTAGCTG	137.59663
BB32905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02878	WGS84	BB32905	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32905_Aamu_Bax2006_COI	PS-A04	1	BB32905_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32905	Sample	BB32905	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A04	COI	45	BB32905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A04	GCACAACCGGGATCTTTACTTC	137.60044
BB32905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02878	WGS84	BB32905	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32905_Pcan_Bot2011_IGS	PS-A04	1	BB32905_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32905	Sample	BB32905	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A04	IGS	45	BB32905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A04	CGTAATCCTCAGTACTGGCTA	137.60044
BB32905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02878	WGS84	BB32905	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.38800049	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32905_Sspa_Oph2007_COI	PS-A04	1	BB32905_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32905	Sample	BB32905	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A04	COI	45	BB32905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A04	GAAGGCCTTCCGATGATC	137.60044
BB32905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02878	WGS84	BB32905	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32905_Upin_Bot2011_COI	PS-A04	1	BB32905_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32905	Sample	BB32905	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A04	COI	45	BB32905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A04	CTTTAATTACAGCGTTTTTATTGTTGT	137.60044
BB32906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03257	WGS84	BB32906	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32906_Mgig_Bot2012_COI	PS-A05	1	BB32906_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32906	Sample	BB32906	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A05	COI	45	BB32906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A05	TCTTATTCGTTGGAGACTTTATAACCCT	137.60012
BB32907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03665	WGS84	BB32907	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32907_Cmae_Bot2011_COI	PS-A06	1	BB32907_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32907	Sample	BB32907	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A06	COI	45	BB32907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A06	ATGAACAGTCTATCCTCCTTTAG	137.59798
BB32907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03665	WGS84	BB32907	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32907_Msal_Bot2012_COI	PS-A06	1	BB32907_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32907	Sample	BB32907	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A06	COI	45	BB32907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A06	GYTAGTTCCRATGATGTTAGCTG	137.59798
BB32909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04109	WGS84	BB32909	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32909_Aamu_Bax2006_COI	PS-A08	1	BB32909_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32909	Sample	BB32909	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A08	COI	45	BB32909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A08	GCACAACCGGGATCTTTACTTC	137.59478
BB32909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04109	WGS84	BB32909	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32909_Pcan_Bot2011_IGS	PS-A08	1	BB32909_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32909	Sample	BB32909	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A08	IGS	45	BB32909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A08	CGTAATCCTCAGTACTGGCTA	137.59478
BB32909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04109	WGS84	BB32909	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.00799942	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32909_Sspa_Oph2007_COI	PS-A08	1	BB32909_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32909	Sample	BB32909	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A08	COI	45	BB32909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A08	GAAGGCCTTCCGATGATC	137.59478
BB32909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04109	WGS84	BB32909	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32909_Upin_Bot2011_COI	PS-A08	1	BB32909_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32909	Sample	BB32909	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A08	COI	45	BB32909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A08	CTTTAATTACAGCGTTTTTATTGTTGT	137.59478
BB32910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03907	WGS84	BB32910	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32910_Cmae_Bot2011_COI	PS-A09	1	BB32910_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32910	Sample	BB32910	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A09	COI	45	BB32910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A09	ATGAACAGTCTATCCTCCTTTAG	137.59498
BB32910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03907	WGS84	BB32910	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32910_Msal_Bot2012_COI	PS-A09	1	BB32910_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32910	Sample	BB32910	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A09	COI	45	BB32910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A09	GYTAGTTCCRATGATGTTAGCTG	137.59498
BB32912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01736	WGS84	BB32912	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32912_Aamu_Bax2006_COI	PS-A11	1	BB32912_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32912	Sample	BB32912	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A11	COI	45	BB32912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A11	GCACAACCGGGATCTTTACTTC	137.60109
BB32912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01736	WGS84	BB32912	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32912_Pcan_Bot2011_IGS	PS-A11	1	BB32912_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32912	Sample	BB32912	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A11	IGS	45	BB32912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A11	CGTAATCCTCAGTACTGGCTA	137.60109
BB32912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01736	WGS84	BB32912	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.08799934	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32912_Sspa_Oph2007_COI	PS-A11	1	BB32912_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32912	Sample	BB32912	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A11	COI	45	BB32912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A11	GAAGGCCTTCCGATGATC	137.60109
BB32912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01736	WGS84	BB32912	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32912_Upin_Bot2011_COI	PS-A11	1	BB32912_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32912	Sample	BB32912	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A11	COI	45	BB32912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A11	CTTTAATTACAGCGTTTTTATTGTTGT	137.60109
BB32913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01847	WGS84	BB32913	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32913_Mgig_Bot2012_COI	PS-A12	1	BB32913_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32913	Sample	BB32913	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A12	COI	45	BB32913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A12	TCTTATTCGTTGGAGACTTTATAACCCT	137.59956
BB32914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01807	WGS84	BB32914	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32914_Cmae_Bot2011_COI	PS-A13	1	BB32914_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32914	Sample	BB32914	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A13	COI	45	BB32914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A13	ATGAACAGTCTATCCTCCTTTAG	137.59817
BB32914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01807	WGS84	BB32914	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32914_Msal_Bot2012_COI	PS-A13	1	BB32914_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32914	Sample	BB32914	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A13	COI	45	BB32914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A13	GYTAGTTCCRATGATGTTAGCTG	137.59817
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01845	WGS84	BB32916	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32916_Aamu_Bax2006_COI	PS-A15	1	BB32916_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	GCACAACCGGGATCTTTACTTC	137.59697
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01845	WGS84	BB32916	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32916_Pcan_Bot2011_IGS	PS-A15	1	BB32916_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A15	IGS	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	CGTAATCCTCAGTACTGGCTA	137.59697
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01845	WGS84	BB32916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.9810009	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32916_Sspa_Oph2007_COI	PS-A15	1	BB32916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	GAAGGCCTTCCGATGATC	137.59697
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01845	WGS84	BB32916	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32916_Upin_Bot2011_COI	PS-A15	1	BB32916_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	CTTTAATTACAGCGTTTTTATTGTTGT	137.59697
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.01845	WGS84	BB32916	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32916_Vgib_Wil2025_COI	PS-A15	1	BB32916_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	CCCCTCTTTACAAGTTCTTTAGGGT	137.59697
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.01982	WGS84	BB32917	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32917_Asen_Wil2025_COI	PS-A16	1	BB32917_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	GTTGTTGTTACAACACATGCTCTTTTG	137.59981
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01982	WGS84	BB32917	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32917_Mgig_Bot2012_COI	PS-A16	1	BB32917_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	TCTTATTCGTTGGAGACTTTATAACCCT	137.59981
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02044	WGS84	BB32918	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32918_Cmae_Bot2011_COI	PS-A17	1	BB32918_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	ATGAACAGTCTATCCTCCTTTAG	137.60071
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02044	WGS84	BB32918	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32918_Msal_Bot2012_COI	PS-A17	1	BB32918_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	GYTAGTTCCRATGATGTTAGCTG	137.60071
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03113	WGS84	BB32920	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32920_Asen_Wil2025_COI	PS-A19	1	BB32920_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	GTTGTTGTTACAACACATGCTCTTTTG	137.60008
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03113	WGS84	BB32920	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32920_Mgig_Bot2012_COI	PS-A19	1	BB32920_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	TCTTATTCGTTGGAGACTTTATAACCCT	137.60008
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03389	WGS84	BB32921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32921_Cmae_Bot2011_COI	PS-A20	1	BB32921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	ATGAACAGTCTATCCTCCTTTAG	137.60019
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03389	WGS84	BB32921	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32921_Msal_Bot2012_COI	PS-A20	1	BB32921_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	GYTAGTTCCRATGATGTTAGCTG	137.60019
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03696	WGS84	BB32923	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32923_Aamu_Bax2006_COI	PS-A22	1	BB32923_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	GCACAACCGGGATCTTTACTTC	137.59708
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03696	WGS84	BB32923	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32923_Pcan_Bot2011_IGS	PS-A22	1	BB32923_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A22	IGS	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	CGTAATCCTCAGTACTGGCTA	137.59708
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03696	WGS84	BB32923	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.44099998	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32923_Sspa_Oph2007_COI	PS-A22	1	BB32923_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	GAAGGCCTTCCGATGATC	137.59708
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03696	WGS84	BB32923	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32923_Upin_Bot2011_COI	PS-A22	1	BB32923_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	CTTTAATTACAGCGTTTTTATTGTTGT	137.59708
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03696	WGS84	BB32923	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32923_Vgib_Wil2025_COI	PS-A22	1	BB32923_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	CCCCTCTTTACAAGTTCTTTAGGGT	137.59708
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03825	WGS84	BB32924	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32924_Asen_Wil2025_COI	PS-A23	1	BB32924_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	GTTGTTGTTACAACACATGCTCTTTTG	137.59652
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03825	WGS84	BB32924	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32924_Mgig_Bot2012_COI	PS-A23	1	BB32924_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	TCTTATTCGTTGGAGACTTTATAACCCT	137.59652
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04269	WGS84	BB32925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32925_Cmae_Bot2011_COI	PS-A24	1	BB32925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	ATGAACAGTCTATCCTCCTTTAG	137.59376
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04269	WGS84	BB32925	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32925_Msal_Bot2012_COI	PS-A24	1	BB32925_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	GYTAGTTCCRATGATGTTAGCTG	137.59376
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04086	WGS84	BB32927	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32927_Aamu_Bax2006_COI	PS-A26	1	BB32927_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	GCACAACCGGGATCTTTACTTC	137.59588
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04086	WGS84	BB32927	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32927_Pcan_Bot2011_IGS	PS-A26	1	BB32927_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A26	IGS	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	CGTAATCCTCAGTACTGGCTA	137.59588
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04086	WGS84	BB32927	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.0340004	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32927_Sspa_Oph2007_COI	PS-A26	1	BB32927_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	GAAGGCCTTCCGATGATC	137.59588
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04086	WGS84	BB32927	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32927_Upin_Bot2011_COI	PS-A26	1	BB32927_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	CTTTAATTACAGCGTTTTTATTGTTGT	137.59588
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04086	WGS84	BB32927	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32927_Vgib_Wil2025_COI	PS-A26	1	BB32927_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	CCCCTCTTTACAAGTTCTTTAGGGT	137.59588
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03928	WGS84	BB32928	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32928_Asen_Wil2025_COI	PS-A27	1	BB32928_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	GTTGTTGTTACAACACATGCTCTTTTG	137.59475
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03928	WGS84	BB32928	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32928_Mgig_Bot2012_COI	PS-A27	1	BB32928_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	TCTTATTCGTTGGAGACTTTATAACCCT	137.59475
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04087	WGS84	BB32929	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32929_Cmae_Bot2011_COI	PS-A28	1	BB32929_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	ATGAACAGTCTATCCTCCTTTAG	137.59434
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04087	WGS84	BB32929	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32929_Msal_Bot2012_COI	PS-A28	1	BB32929_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	GYTAGTTCCRATGATGTTAGCTG	137.59434
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03918	WGS84	BB32930	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32930_Aamu_Bax2006_COI	PS-A29	1	BB32930_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	GCACAACCGGGATCTTTACTTC	137.59354
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03918	WGS84	BB32930	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32930_Pcan_Bot2011_IGS	PS-A29	1	BB32930_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A29	IGS	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	CGTAATCCTCAGTACTGGCTA	137.59354
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03918	WGS84	BB32930	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.06000137	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32930_Sspa_Oph2007_COI	PS-A29	1	BB32930_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	GAAGGCCTTCCGATGATC	137.59354
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03918	WGS84	BB32930	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32930_Upin_Bot2011_COI	PS-A29	1	BB32930_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	CTTTAATTACAGCGTTTTTATTGTTGT	137.59354
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03918	WGS84	BB32930	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32930_Vgib_Wil2025_COI	PS-A29	1	BB32930_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	CCCCTCTTTACAAGTTCTTTAGGGT	137.59354
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04053	WGS84	BB32931	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32931_Asen_Wil2025_COI	PS-A30	1	BB32931_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	GTTGTTGTTACAACACATGCTCTTTTG	137.59253
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04053	WGS84	BB32931	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32931_Mgig_Bot2012_COI	PS-A30	1	BB32931_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	TCTTATTCGTTGGAGACTTTATAACCCT	137.59253
BB32938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72023	WGS84	BB32938	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32938_Aamu_Bax2006_COI	PS-B02	1	BB32938_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32938	Sample	BB32938	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B02	COI	45	BB32938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B02	GCACAACCGGGATCTTTACTTC	135.86471
BB32938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72023	WGS84	BB32938	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32938_Pcan_Bot2011_IGS	PS-B02	1	BB32938_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32938	Sample	BB32938	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B02	IGS	45	BB32938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B02	CGTAATCCTCAGTACTGGCTA	135.86471
BB32938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72023	WGS84	BB32938	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.59400177	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32938_Sspa_Oph2007_COI	PS-B02	1	BB32938_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32938	Sample	BB32938	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B02	COI	45	BB32938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B02	GAAGGCCTTCCGATGATC	135.86471
BB32938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72023	WGS84	BB32938	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32938_Upin_Bot2011_COI	PS-B02	1	BB32938_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32938	Sample	BB32938	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B02	COI	45	BB32938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B02	CTTTAATTACAGCGTTTTTATTGTTGT	135.86471
BB32939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71791	WGS84	BB32939	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32939_Mgig_Bot2012_COI	PS-B03	1	BB32939_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32939	Sample	BB32939	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B03	COI	45	BB32939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B03	TCTTATTCGTTGGAGACTTTATAACCCT	135.86262
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71512	WGS84	BB32941	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32941_Aamu_Bax2006_COI	PS-B05	1	BB32941_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	GCACAACCGGGATCTTTACTTC	135.87171
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71512	WGS84	BB32941	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32941_Pcan_Bot2011_IGS	PS-B05	1	BB32941_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B05	IGS	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	CGTAATCCTCAGTACTGGCTA	135.87171
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71512	WGS84	BB32941	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32941_Sspa_Oph2007_COI	PS-B05	1	BB32941_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	GAAGGCCTTCCGATGATC	135.87171
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71512	WGS84	BB32941	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32941_Upin_Bot2011_COI	PS-B05	1	BB32941_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	CTTTAATTACAGCGTTTTTATTGTTGT	135.87171
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71512	WGS84	BB32941	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32941_Vgib_Wil2025_COI	PS-B05	1	BB32941_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	CCCCTCTTTACAAGTTCTTTAGGGT	135.87171
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71875	WGS84	BB32942	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32942_Asen_Wil2025_COI	PS-B06	1	BB32942_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	GTTGTTGTTACAACACATGCTCTTTTG	135.87153
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71875	WGS84	BB32942	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32942_Mgig_Bot2012_COI	PS-B06	1	BB32942_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	TCTTATTCGTTGGAGACTTTATAACCCT	135.87153
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72029	WGS84	BB32943	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32943_Cmae_Bot2011_COI	PS-B07	1	BB32943_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	ATGAACAGTCTATCCTCCTTTAG	135.87318
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72029	WGS84	BB32943	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32943_Msal_Bot2012_COI	PS-B07	1	BB32943_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	GYTAGTTCCRATGATGTTAGCTG	135.87318
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72015	WGS84	BB32945	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32945_Aamu_Bax2006_COI	PS-B09	1	BB32945_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	GCACAACCGGGATCTTTACTTC	135.8806
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72015	WGS84	BB32945	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32945_Pcan_Bot2011_IGS	PS-B09	1	BB32945_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B09	IGS	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	CGTAATCCTCAGTACTGGCTA	135.8806
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72015	WGS84	BB32945	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32945_Sspa_Oph2007_COI	PS-B09	1	BB32945_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	GAAGGCCTTCCGATGATC	135.8806
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72015	WGS84	BB32945	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32945_Upin_Bot2011_COI	PS-B09	1	BB32945_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	CTTTAATTACAGCGTTTTTATTGTTGT	135.8806
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.72015	WGS84	BB32945	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32945_Vgib_Wil2025_COI	PS-B09	1	BB32945_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	CCCCTCTTTACAAGTTCTTTAGGGT	135.8806
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.72483	WGS84	BB32946	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32946_Asen_Wil2025_COI	PS-B10	1	BB32946_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	GTTGTTGTTACAACACATGCTCTTTTG	135.88569
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72483	WGS84	BB32946	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32946_Mgig_Bot2012_COI	PS-B10	1	BB32946_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	TCTTATTCGTTGGAGACTTTATAACCCT	135.88569
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71863	WGS84	BB32947	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32947_Cmae_Bot2011_COI	PS-B11	1	BB32947_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	ATGAACAGTCTATCCTCCTTTAG	135.86087
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71863	WGS84	BB32947	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32947_Msal_Bot2012_COI	PS-B11	1	BB32947_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	GYTAGTTCCRATGATGTTAGCTG	135.86087
BB32949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72033	WGS84	BB32949	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32949_Aamu_Bax2006_COI	PS-B13	1	BB32949_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32949	Sample	BB32949	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B13	COI	45	BB32949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B13	GCACAACCGGGATCTTTACTTC	135.86433
BB32949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72033	WGS84	BB32949	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32949_Pcan_Bot2011_IGS	PS-B13	1	BB32949_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32949	Sample	BB32949	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B13	IGS	45	BB32949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B13	CGTAATCCTCAGTACTGGCTA	135.86433
BB32949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72033	WGS84	BB32949	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.15200043	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32949_Sspa_Oph2007_COI	PS-B13	1	BB32949_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32949	Sample	BB32949	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B13	COI	45	BB32949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B13	GAAGGCCTTCCGATGATC	135.86433
BB32949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72033	WGS84	BB32949	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32949_Upin_Bot2011_COI	PS-B13	1	BB32949_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32949	Sample	BB32949	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B13	COI	45	BB32949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B13	CTTTAATTACAGCGTTTTTATTGTTGT	135.86433
BB32950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72022	WGS84	BB32950	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32950_Cmae_Bot2011_COI	PS-B14	1	BB32950_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32950	Sample	BB32950	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B14	COI	45	BB32950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B14	ATGAACAGTCTATCCTCCTTTAG	135.86322
BB32950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72022	WGS84	BB32950	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32950_Msal_Bot2012_COI	PS-B14	1	BB32950_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32950	Sample	BB32950	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B14	COI	45	BB32950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B14	GYTAGTTCCRATGATGTTAGCTG	135.86322
BB32952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71716	WGS84	BB32952	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32952_Aamu_Bax2006_COI	PS-B16	1	BB32952_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32952	Sample	BB32952	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B16	COI	45	BB32952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B16	GCACAACCGGGATCTTTACTTC	135.8641
BB32952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71716	WGS84	BB32952	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32952_Pcan_Bot2011_IGS	PS-B16	1	BB32952_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32952	Sample	BB32952	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B16	IGS	45	BB32952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B16	CGTAATCCTCAGTACTGGCTA	135.8641
BB32952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71716	WGS84	BB32952	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32952_Sspa_Oph2007_COI	PS-B16	1	BB32952_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32952	Sample	BB32952	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B16	COI	45	BB32952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B16	GAAGGCCTTCCGATGATC	135.8641
BB32952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71716	WGS84	BB32952	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32952_Upin_Bot2011_COI	PS-B16	1	BB32952_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32952	Sample	BB32952	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B16	COI	45	BB32952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B16	CTTTAATTACAGCGTTTTTATTGTTGT	135.8641
BB32953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7164	WGS84	BB32953	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32953_Mgig_Bot2012_COI	PS-B17	1	BB32953_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32953	Sample	BB32953	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B17	COI	45	BB32953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B17	TCTTATTCGTTGGAGACTTTATAACCCT	135.86547
BB32954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71403	WGS84	BB32954	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32954_Cmae_Bot2011_COI	PS-B18	1	BB32954_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32954	Sample	BB32954	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B18	COI	45	BB32954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B18	ATGAACAGTCTATCCTCCTTTAG	135.86807
BB32954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71403	WGS84	BB32954	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32954_Msal_Bot2012_COI	PS-B18	1	BB32954_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32954	Sample	BB32954	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B18	COI	45	BB32954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B18	GYTAGTTCCRATGATGTTAGCTG	135.86807
BB32956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71632	WGS84	BB32956	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32956_Aamu_Bax2006_COI	PS-B20	1	BB32956_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32956	Sample	BB32956	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B20	COI	45	BB32956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B20	GCACAACCGGGATCTTTACTTC	135.87171
BB32956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71632	WGS84	BB32956	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32956_Pcan_Bot2011_IGS	PS-B20	1	BB32956_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32956	Sample	BB32956	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B20	IGS	45	BB32956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B20	CGTAATCCTCAGTACTGGCTA	135.87171
BB32956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71632	WGS84	BB32956	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32956_Sspa_Oph2007_COI	PS-B20	1	BB32956_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32956	Sample	BB32956	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B20	COI	45	BB32956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B20	GAAGGCCTTCCGATGATC	135.87171
BB32956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71632	WGS84	BB32956	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32956_Upin_Bot2011_COI	PS-B20	1	BB32956_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32956	Sample	BB32956	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B20	COI	45	BB32956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B20	CTTTAATTACAGCGTTTTTATTGTTGT	135.87171
BB32957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7175	WGS84	BB32957	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32957_Mgig_Bot2012_COI	PS-B21	1	BB32957_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32957	Sample	BB32957	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B21	COI	45	BB32957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B21	TCTTATTCGTTGGAGACTTTATAACCCT	135.87182
BB32958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72017	WGS84	BB32958	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32958_Cmae_Bot2011_COI	PS-B22	1	BB32958_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32958	Sample	BB32958	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B22	COI	45	BB32958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B22	ATGAACAGTCTATCCTCCTTTAG	135.87103
BB32958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72017	WGS84	BB32958	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32958_Msal_Bot2012_COI	PS-B22	1	BB32958_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32958	Sample	BB32958	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B22	COI	45	BB32958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B22	GYTAGTTCCRATGATGTTAGCTG	135.87103
BB32960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71918	WGS84	BB32960	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32960_Mgig_Bot2012_COI	PS-B24	1	BB32960_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32960	Sample	BB32960	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B24	COI	45	BB32960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B24	TCTTATTCGTTGGAGACTTTATAACCCT	135.87416
BB32961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71855	WGS84	BB32961	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32961_Cmae_Bot2011_COI	PS-B25	1	BB32961_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32961	Sample	BB32961	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B25	COI	45	BB32961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B25	ATGAACAGTCTATCCTCCTTTAG	135.87565
BB32961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71855	WGS84	BB32961	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32961_Msal_Bot2012_COI	PS-B25	1	BB32961_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32961	Sample	BB32961	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B25	COI	45	BB32961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B25	GYTAGTTCCRATGATGTTAGCTG	135.87565
BB32963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71932	WGS84	BB32963	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32963_Aamu_Bax2006_COI	PS-B27	1	BB32963_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32963	Sample	BB32963	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B27	COI	45	BB32963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B27	GCACAACCGGGATCTTTACTTC	135.87964
BB32963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71932	WGS84	BB32963	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32963_Pcan_Bot2011_IGS	PS-B27	1	BB32963_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32963	Sample	BB32963	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B27	IGS	45	BB32963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B27	CGTAATCCTCAGTACTGGCTA	135.87964
BB32963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71932	WGS84	BB32963	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32963_Sspa_Oph2007_COI	PS-B27	1	BB32963_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32963	Sample	BB32963	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B27	COI	45	BB32963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B27	GAAGGCCTTCCGATGATC	135.87964
BB32963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71932	WGS84	BB32963	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32963_Upin_Bot2011_COI	PS-B27	1	BB32963_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32963	Sample	BB32963	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B27	COI	45	BB32963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B27	CTTTAATTACAGCGTTTTTATTGTTGT	135.87964
BB32964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.723	WGS84	BB32964	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32964_Mgig_Bot2012_COI	PS-B28	1	BB32964_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32964	Sample	BB32964	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B28	COI	45	BB32964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B28	TCTTATTCGTTGGAGACTTTATAACCCT	135.88382
BB32965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72362	WGS84	BB32965	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32965_Cmae_Bot2011_COI	PS-B29	1	BB32965_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32965	Sample	BB32965	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B29	COI	45	BB32965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B29	ATGAACAGTCTATCCTCCTTTAG	135.88477
BB32965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72362	WGS84	BB32965	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32965_Msal_Bot2012_COI	PS-B29	1	BB32965_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32965	Sample	BB32965	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B29	COI	45	BB32965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B29	GYTAGTTCCRATGATGTTAGCTG	135.88477
BB32972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93052	WGS84	BB32972	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32972_Cmae_Bot2011_COI	PS-C01	1	BB32972_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32972	Sample	BB32972	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C01	COI	45	BB32972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C01	ATGAACAGTCTATCCTCCTTTAG	137.61115
BB32972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93052	WGS84	BB32972	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32972_Msal_Bot2012_COI	PS-C01	1	BB32972_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32972	Sample	BB32972	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C01	COI	45	BB32972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C01	GYTAGTTCCRATGATGTTAGCTG	137.61115
BB32974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92647	WGS84	BB32974	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32974_Aamu_Bax2006_COI	PS-C03	1	BB32974_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32974	Sample	BB32974	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C03	COI	45	BB32974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C03	GCACAACCGGGATCTTTACTTC	137.61272
BB32974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92647	WGS84	BB32974	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32974_Pcan_Bot2011_IGS	PS-C03	1	BB32974_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32974	Sample	BB32974	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C03	IGS	45	BB32974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C03	CGTAATCCTCAGTACTGGCTA	137.61272
BB32974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92647	WGS84	BB32974	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32974_Sspa_Oph2007_COI	PS-C03	1	BB32974_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32974	Sample	BB32974	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C03	COI	45	BB32974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C03	GAAGGCCTTCCGATGATC	137.61272
BB32974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92647	WGS84	BB32974	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32974_Upin_Bot2011_COI	PS-C03	1	BB32974_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32974	Sample	BB32974	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C03	COI	45	BB32974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C03	CTTTAATTACAGCGTTTTTATTGTTGT	137.61272
BB32975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92373	WGS84	BB32975	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32975_Mgig_Bot2012_COI	PS-C04	1	BB32975_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32975	Sample	BB32975	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C04	COI	45	BB32975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C04	TCTTATTCGTTGGAGACTTTATAACCCT	137.61315
BB32976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92643	WGS84	BB32976	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32976_Cmae_Bot2011_COI	PS-C05	1	BB32976_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32976	Sample	BB32976	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C05	COI	45	BB32976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C05	ATGAACAGTCTATCCTCCTTTAG	137.62
BB32976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92643	WGS84	BB32976	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32976_Msal_Bot2012_COI	PS-C05	1	BB32976_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32976	Sample	BB32976	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C05	COI	45	BB32976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C05	GYTAGTTCCRATGATGTTAGCTG	137.62
BB32978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92124	WGS84	BB32978	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32978_Aamu_Bax2006_COI	PS-C07	1	BB32978_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32978	Sample	BB32978	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C07	COI	45	BB32978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C07	GCACAACCGGGATCTTTACTTC	137.62582
BB32978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92124	WGS84	BB32978	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32978_Pcan_Bot2011_IGS	PS-C07	1	BB32978_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32978	Sample	BB32978	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C07	IGS	45	BB32978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C07	CGTAATCCTCAGTACTGGCTA	137.62582
BB32978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92124	WGS84	BB32978	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32978_Sspa_Oph2007_COI	PS-C07	1	BB32978_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32978	Sample	BB32978	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C07	COI	45	BB32978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C07	GAAGGCCTTCCGATGATC	137.62582
BB32978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92124	WGS84	BB32978	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32978_Upin_Bot2011_COI	PS-C07	1	BB32978_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32978	Sample	BB32978	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C07	COI	45	BB32978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C07	CTTTAATTACAGCGTTTTTATTGTTGT	137.62582
BB32979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92229	WGS84	BB32979	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32979_Mgig_Bot2012_COI	PS-C08	1	BB32979_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32979	Sample	BB32979	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C08	COI	45	BB32979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C08	TCTTATTCGTTGGAGACTTTATAACCCT	137.62767
BB32981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92123	WGS84	BB32981	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32981_Aamu_Bax2006_COI	PS-C10	1	BB32981_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32981	Sample	BB32981	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C10	COI	45	BB32981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C10	GCACAACCGGGATCTTTACTTC	137.63952
BB32981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92123	WGS84	BB32981	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32981_Pcan_Bot2011_IGS	PS-C10	1	BB32981_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32981	Sample	BB32981	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C10	IGS	45	BB32981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C10	CGTAATCCTCAGTACTGGCTA	137.63952
BB32981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92123	WGS84	BB32981	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32981_Sspa_Oph2007_COI	PS-C10	1	BB32981_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32981	Sample	BB32981	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C10	COI	45	BB32981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C10	GAAGGCCTTCCGATGATC	137.63952
BB32981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92123	WGS84	BB32981	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32981_Upin_Bot2011_COI	PS-C10	1	BB32981_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32981	Sample	BB32981	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C10	COI	45	BB32981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C10	CTTTAATTACAGCGTTTTTATTGTTGT	137.63952
BB32982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92987	WGS84	BB32982	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32982_Mgig_Bot2012_COI	PS-C11	1	BB32982_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32982	Sample	BB32982	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C11	COI	45	BB32982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C11	TCTTATTCGTTGGAGACTTTATAACCCT	137.61215
BB32983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93096	WGS84	BB32983	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32983_Cmae_Bot2011_COI	PS-C12	1	BB32983_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32983	Sample	BB32983	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C12	COI	45	BB32983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C12	ATGAACAGTCTATCCTCCTTTAG	137.61016
BB32983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93096	WGS84	BB32983	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32983_Msal_Bot2012_COI	PS-C12	1	BB32983_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32983	Sample	BB32983	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C12	COI	45	BB32983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C12	GYTAGTTCCRATGATGTTAGCTG	137.61016
BB32985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93162	WGS84	BB32985	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32985_Aamu_Bax2006_COI	PS-C14	1	BB32985_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32985	Sample	BB32985	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C14	COI	45	BB32985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C14	GCACAACCGGGATCTTTACTTC	137.60666
BB32985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93162	WGS84	BB32985	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32985_Pcan_Bot2011_IGS	PS-C14	1	BB32985_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32985	Sample	BB32985	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C14	IGS	45	BB32985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C14	CGTAATCCTCAGTACTGGCTA	137.60666
BB32985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93162	WGS84	BB32985	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32985_Sspa_Oph2007_COI	PS-C14	1	BB32985_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32985	Sample	BB32985	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C14	COI	45	BB32985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C14	GAAGGCCTTCCGATGATC	137.60666
BB32985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93162	WGS84	BB32985	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32985_Upin_Bot2011_COI	PS-C14	1	BB32985_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32985	Sample	BB32985	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C14	COI	45	BB32985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C14	CTTTAATTACAGCGTTTTTATTGTTGT	137.60666
BB32986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92686	WGS84	BB32986	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32986_Mgig_Bot2012_COI	PS-C15	1	BB32986_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32986	Sample	BB32986	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C15	COI	45	BB32986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C15	TCTTATTCGTTGGAGACTTTATAACCCT	137.61371
BB32987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92631	WGS84	BB32987	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32987_Cmae_Bot2011_COI	PS-C16	1	BB32987_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32987	Sample	BB32987	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C16	COI	45	BB32987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C16	ATGAACAGTCTATCCTCCTTTAG	137.6116
BB32987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92631	WGS84	BB32987	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32987_Msal_Bot2012_COI	PS-C16	1	BB32987_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32987	Sample	BB32987	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C16	COI	45	BB32987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C16	GYTAGTTCCRATGATGTTAGCTG	137.6116
BB32989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92406	WGS84	BB32989	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32989_Aamu_Bax2006_COI	PS-C18	1	BB32989_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32989	Sample	BB32989	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C18	COI	45	BB32989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C18	GCACAACCGGGATCTTTACTTC	137.61464
BB32989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92406	WGS84	BB32989	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32989_Pcan_Bot2011_IGS	PS-C18	1	BB32989_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32989	Sample	BB32989	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C18	IGS	45	BB32989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C18	CGTAATCCTCAGTACTGGCTA	137.61464
BB32989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92406	WGS84	BB32989	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32989_Sspa_Oph2007_COI	PS-C18	1	BB32989_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32989	Sample	BB32989	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C18	COI	45	BB32989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C18	GAAGGCCTTCCGATGATC	137.61464
BB32989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92406	WGS84	BB32989	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32989_Upin_Bot2011_COI	PS-C18	1	BB32989_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32989	Sample	BB32989	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C18	COI	45	BB32989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C18	CTTTAATTACAGCGTTTTTATTGTTGT	137.61464
BB32990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92591	WGS84	BB32990	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32990_Cmae_Bot2011_COI	PS-C19	1	BB32990_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32990	Sample	BB32990	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C19	COI	45	BB32990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C19	ATGAACAGTCTATCCTCCTTTAG	137.61858
BB32990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92591	WGS84	BB32990	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32990_Msal_Bot2012_COI	PS-C19	1	BB32990_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32990	Sample	BB32990	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C19	COI	45	BB32990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C19	GYTAGTTCCRATGATGTTAGCTG	137.61858
BB32992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92572	WGS84	BB32992	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32992_Aamu_Bax2006_COI	PS-C21	1	BB32992_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32992	Sample	BB32992	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C21	COI	45	BB32992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C21	GCACAACCGGGATCTTTACTTC	137.62211
BB32992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92572	WGS84	BB32992	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32992_Pcan_Bot2011_IGS	PS-C21	1	BB32992_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32992	Sample	BB32992	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C21	IGS	45	BB32992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C21	CGTAATCCTCAGTACTGGCTA	137.62211
BB32992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92572	WGS84	BB32992	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32992_Sspa_Oph2007_COI	PS-C21	1	BB32992_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32992	Sample	BB32992	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C21	COI	45	BB32992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C21	GAAGGCCTTCCGATGATC	137.62211
BB32992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92572	WGS84	BB32992	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32992_Upin_Bot2011_COI	PS-C21	1	BB32992_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32992	Sample	BB32992	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C21	COI	45	BB32992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C21	CTTTAATTACAGCGTTTTTATTGTTGT	137.62211
BB32993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92419	WGS84	BB32993	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32993_Mgig_Bot2012_COI	PS-C22	1	BB32993_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32993	Sample	BB32993	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C22	COI	45	BB32993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C22	TCTTATTCGTTGGAGACTTTATAACCCT	137.62419
BB32994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92304	WGS84	BB32994	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32994_Cmae_Bot2011_COI	PS-C23	1	BB32994_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32994	Sample	BB32994	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C23	COI	45	BB32994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C23	ATGAACAGTCTATCCTCCTTTAG	137.62427
BB32994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92304	WGS84	BB32994	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32994_Msal_Bot2012_COI	PS-C23	1	BB32994_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32994	Sample	BB32994	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C23	COI	45	BB32994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C23	GYTAGTTCCRATGATGTTAGCTG	137.62427
BB32996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92203	WGS84	BB32996	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32996_Aamu_Bax2006_COI	PS-C25	1	BB32996_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32996	Sample	BB32996	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C25	COI	45	BB32996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C25	GCACAACCGGGATCTTTACTTC	137.62572
BB32996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92203	WGS84	BB32996	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32996_Pcan_Bot2011_IGS	PS-C25	1	BB32996_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32996	Sample	BB32996	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C25	IGS	45	BB32996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C25	CGTAATCCTCAGTACTGGCTA	137.62572
BB32996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92203	WGS84	BB32996	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32996_Sspa_Oph2007_COI	PS-C25	1	BB32996_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32996	Sample	BB32996	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C25	COI	45	BB32996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C25	GAAGGCCTTCCGATGATC	137.62572
BB32996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92203	WGS84	BB32996	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32996_Upin_Bot2011_COI	PS-C25	1	BB32996_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32996	Sample	BB32996	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C25	COI	45	BB32996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C25	CTTTAATTACAGCGTTTTTATTGTTGT	137.62572
BB32997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92254	WGS84	BB32997	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32997_Mgig_Bot2012_COI	PS-C26	1	BB32997_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32997	Sample	BB32997	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C26	COI	45	BB32997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C26	TCTTATTCGTTGGAGACTTTATAACCCT	137.62948
BB32998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92361	WGS84	BB32998	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32998_Cmae_Bot2011_COI	PS-C27	1	BB32998_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32998	Sample	BB32998	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C27	COI	45	BB32998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C27	ATGAACAGTCTATCCTCCTTTAG	137.63321
BB32998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92361	WGS84	BB32998	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32998_Msal_Bot2012_COI	PS-C27	1	BB32998_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32998	Sample	BB32998	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C27	COI	45	BB32998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C27	GYTAGTTCCRATGATGTTAGCTG	137.63321
BB33000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92206	WGS84	BB33000	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB33000_Mgig_Bot2012_COI	PS-C29	1	BB33000_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33000	Sample	BB33000	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C29	COI	45	BB33000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C29	TCTTATTCGTTGGAGACTTTATAACCCT	137.63842
BB33001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.91952	WGS84	BB33001	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB33001_Cmae_Bot2011_COI	PS-C30	1	BB33001_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33001	Sample	BB33001	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C30	COI	45	BB33001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C30	ATGAACAGTCTATCCTCCTTTAG	137.63993
BB33001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.91952	WGS84	BB33001	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB33001_Msal_Bot2012_COI	PS-C30	1	BB33001_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33001	Sample	BB33001	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C30	COI	45	BB33001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C30	GYTAGTTCCRATGATGTTAGCTG	137.63993
BB46878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84869	WGS84	BB46878	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.433	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46878_Aamu_Bax2006_COI	M37	1	BB46878_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46878	Sample	BB46878	targeted	Present	Sample	Aamu_Bax2006_COI	M37	COI	45	BB46878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M37	GCACAACCGGGATCTTTACTTC	144.9162
BB46878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84869	WGS84	BB46878	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.110016	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46878_Sspa_Oph2007_COI	M37	1	BB46878_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46878	Sample	BB46878	targeted	Present	Sample	Sspa_Oph2007_COI	M37	COI	45	BB46878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M37	GAAGGCCTTCCGATGATC	144.9162
BB46878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84869	WGS84	BB46878	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.641	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46878_Upin_Bot2011_COI	M37	1	BB46878_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46878	Sample	BB46878	targeted	Present	Sample	Upin_Bot2011_COI	M37	COI	45	BB46878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M37	CTTTAATTACAGCGTTTTTATTGTTGT	144.9162
BB46881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84349	WGS84	BB46881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.965	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46881_Aamu_Bax2006_COI	M40	1	BB46881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46881	Sample	BB46881	targeted	Present	Sample	Aamu_Bax2006_COI	M40	COI	45	BB46881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M40	GCACAACCGGGATCTTTACTTC	144.90042
BB46881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84349	WGS84	BB46881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.751675	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46881_Sspa_Oph2007_COI	M40	1	BB46881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46881	Sample	BB46881	targeted	Present	Sample	Sspa_Oph2007_COI	M40	COI	45	BB46881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M40	GAAGGCCTTCCGATGATC	144.90042
BB46881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84349	WGS84	BB46881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.178	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46881_Upin_Bot2011_COI	M40	1	BB46881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46881	Sample	BB46881	targeted	Present	Sample	Upin_Bot2011_COI	M40	COI	45	BB46881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M40	CTTTAATTACAGCGTTTTTATTGTTGT	144.90042
BB46885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.82076	WGS84	BB46885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.595642	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46885_Sspa_Oph2007_COI	M44	1	BB46885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46885	Sample	BB46885	targeted	Present	Sample	Sspa_Oph2007_COI	M44	COI	45	BB46885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M44	GAAGGCCTTCCGATGATC	144.9125
BB46887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.83822	WGS84	BB46887	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.6	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB46887_Cmae_Bot2011_COI	M46	1	BB46887_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46887	Sample	BB46887	targeted	Present	Sample	Cmae_Bot2011_COI	M46	COI	45	BB46887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M46	ATGAACAGTCTATCCTCCTTTAG	144.89792
BB46889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84242	WGS84	BB46889	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.921	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46889_Aamu_Bax2006_COI	M48	1	BB46889_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46889	Sample	BB46889	targeted	Present	Sample	Aamu_Bax2006_COI	M48	COI	45	BB46889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M48	GCACAACCGGGATCTTTACTTC	144.92887
BB46889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84242	WGS84	BB46889	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.49913	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46889_Sspa_Oph2007_COI	M48	1	BB46889_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46889	Sample	BB46889	targeted	Present	Sample	Sspa_Oph2007_COI	M48	COI	45	BB46889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M48	GAAGGCCTTCCGATGATC	144.92887
BB46889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84242	WGS84	BB46889	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.565	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46889_Upin_Bot2011_COI	M48	1	BB46889_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46889	Sample	BB46889	targeted	Present	Sample	Upin_Bot2011_COI	M48	COI	45	BB46889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M48	CTTTAATTACAGCGTTTTTATTGTTGT	144.92887
BB46890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84857	WGS84	BB46890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.457	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB46890_Cmae_Bot2011_COI	M49	1	BB46890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46890	Sample	BB46890	targeted	Present	Sample	Cmae_Bot2011_COI	M49	COI	45	BB46890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M49	ATGAACAGTCTATCCTCCTTTAG	144.91035
BB46892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.8193	WGS84	BB46892	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.651512	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46892_Sspa_Oph2007_COI	M51	1	BB46892_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46892	Sample	BB46892	targeted	Present	Sample	Sspa_Oph2007_COI	M51	COI	45	BB46892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M51	GAAGGCCTTCCGATGATC	144.91134
BB46892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.8193	WGS84	BB46892	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.133	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46892_Upin_Bot2011_COI	M51	1	BB46892_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46892	Sample	BB46892	targeted	Present	Sample	Upin_Bot2011_COI	M51	COI	45	BB46892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M51	CTTTAATTACAGCGTTTTTATTGTTGT	144.91134
BB46894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84244	WGS84	BB46894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.005	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB46894_Cmae_Bot2011_COI	M53	1	BB46894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46894	Sample	BB46894	targeted	Present	Sample	Cmae_Bot2011_COI	M53	COI	45	BB46894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M53	ATGAACAGTCTATCCTCCTTTAG	144.92301
BB46896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.82346	WGS84	BB46896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.487854	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46896_Sspa_Oph2007_COI	M55	1	BB46896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46896	Sample	BB46896	targeted	Present	Sample	Sspa_Oph2007_COI	M55	COI	45	BB46896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M55	GAAGGCCTTCCGATGATC	144.90545
BB57685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77745	WGS84	BB57685	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57685_Cmae_Bot2011_COI	S1-02	1	BB57685_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57685	Sample	BB57685	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-02	COI	45	BB57685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-02	ATGAACAGTCTATCCTCCTTTAG	138.51629
BB57687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84686	WGS84	BB57687	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57687_Aamu_Bax2006_COI	S1-07	1	BB57687_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57687	Sample	BB57687	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-07	COI	45	BB57687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-07	GCACAACCGGGATCTTTACTTC	138.49747
BB57687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84686	WGS84	BB57687	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57687_Pcan_Bot2011_IGS	S1-07	1	BB57687_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57687	Sample	BB57687	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-07	IGS	45	BB57687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-07	CGTAATCCTCAGTACTGGCTA	138.49747
BB57687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84686	WGS84	BB57687	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.315	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57687_Sspa_Oph2007_COI	S1-07	1	BB57687_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57687	Sample	BB57687	targeted	Present	Sample	Sspa_Oph2007_COI	S1-07	COI	45	BB57687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-07	GAAGGCCTTCCGATGATC	138.49747
BB57687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84686	WGS84	BB57687	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57687_Upin_Bot2011_COI	S1-07	1	BB57687_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57687	Sample	BB57687	targeted	Absent	Sample	Upin_Bot2011_COI	S1-07	COI	45	BB57687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.49747
BB57688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8455	WGS84	BB57688	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57688_Cjap_Sim2018_COI	S1-09	1	BB57688_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57688	Sample	BB57688	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-09	COI	45	BB57688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49684
BB57688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.8455	WGS84	BB57688	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.37	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57688_Mgig_Bot2012_COI	S1-09	1	BB57688_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57688	Sample	BB57688	targeted	Present	Sample	Mgig_Bot2012_COI	S1-09	COI	45	BB57688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.49684
BB57689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8371	WGS84	BB57689	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57689_Cmae_Bot2011_COI	S1-12	1	BB57689_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57689	Sample	BB57689	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-12	COI	45	BB57689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-12	ATGAACAGTCTATCCTCCTTTAG	138.5104
BB57690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8365	WGS84	BB57690	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57690_Aamu_Bax2006_COI	S1-14	1	BB57690_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57690	Sample	BB57690	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-14	COI	45	BB57690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-14	GCACAACCGGGATCTTTACTTC	138.50875
BB57690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8365	WGS84	BB57690	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57690_Pcan_Bot2011_IGS	S1-14	1	BB57690_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57690	Sample	BB57690	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-14	IGS	45	BB57690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-14	CGTAATCCTCAGTACTGGCTA	138.50875
BB57690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8365	WGS84	BB57690	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57690_Sspa_Oph2007_COI	S1-14	1	BB57690_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57690	Sample	BB57690	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-14	COI	45	BB57690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-14	GAAGGCCTTCCGATGATC	138.50875
BB57690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8365	WGS84	BB57690	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57690_Upin_Bot2011_COI	S1-14	1	BB57690_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57690	Sample	BB57690	targeted	Absent	Sample	Upin_Bot2011_COI	S1-14	COI	45	BB57690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.50875
BB57691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80941	WGS84	BB57691	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57691_Cjap_Sim2018_COI	S1-17	1	BB57691_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57691	Sample	BB57691	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-17	COI	45	BB57691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52052
BB57691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80941	WGS84	BB57691	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.262	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57691_Mgig_Bot2012_COI	S1-17	1	BB57691_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57691	Sample	BB57691	targeted	Present	Sample	Mgig_Bot2012_COI	S1-17	COI	45	BB57691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.52052
BB57692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81094	WGS84	BB57692	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57692_Cmae_Bot2011_COI	S1-19	1	BB57692_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57692	Sample	BB57692	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-19	COI	45	BB57692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-19	ATGAACAGTCTATCCTCCTTTAG	138.52211
BB57694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7783	WGS84	BB57694	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57694_Aamu_Bax2006_COI	S1-03	1	BB57694_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57694	Sample	BB57694	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-03	COI	45	BB57694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-03	GCACAACCGGGATCTTTACTTC	138.51642
BB57694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7783	WGS84	BB57694	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57694_Pcan_Bot2011_IGS	S1-03	1	BB57694_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57694	Sample	BB57694	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-03	IGS	45	BB57694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-03	CGTAATCCTCAGTACTGGCTA	138.51642
BB57694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7783	WGS84	BB57694	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57694_Sspa_Oph2007_COI	S1-03	1	BB57694_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57694	Sample	BB57694	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-03	COI	45	BB57694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-03	GAAGGCCTTCCGATGATC	138.51642
BB57694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7783	WGS84	BB57694	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57694_Upin_Bot2011_COI	S1-03	1	BB57694_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57694	Sample	BB57694	targeted	Absent	Sample	Upin_Bot2011_COI	S1-03	COI	45	BB57694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.51642
BB57695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78088	WGS84	BB57695	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57695_Cjap_Sim2018_COI	S1-05	1	BB57695_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57695	Sample	BB57695	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-05	COI	45	BB57695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51745
BB57695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78088	WGS84	BB57695	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.835	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57695_Mgig_Bot2012_COI	S1-05	1	BB57695_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57695	Sample	BB57695	targeted	Present	Sample	Mgig_Bot2012_COI	S1-05	COI	45	BB57695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.51745
BB57696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84503	WGS84	BB57696	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57696_Cmae_Bot2011_COI	S1-06	1	BB57696_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57696	Sample	BB57696	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-06	COI	45	BB57696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-06	ATGAACAGTCTATCCTCCTTTAG	138.49721
BB57698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84408	WGS84	BB57698	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57698_Aamu_Bax2006_COI	S1-10	1	BB57698_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57698	Sample	BB57698	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-10	COI	45	BB57698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-10	GCACAACCGGGATCTTTACTTC	138.49724
BB57698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84408	WGS84	BB57698	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57698_Pcan_Bot2011_IGS	S1-10	1	BB57698_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57698	Sample	BB57698	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-10	IGS	45	BB57698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-10	CGTAATCCTCAGTACTGGCTA	138.49724
BB57698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84408	WGS84	BB57698	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.084	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57698_Sspa_Oph2007_COI	S1-10	1	BB57698_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57698	Sample	BB57698	targeted	Present	Sample	Sspa_Oph2007_COI	S1-10	COI	45	BB57698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-10	GAAGGCCTTCCGATGATC	138.49724
BB57698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84408	WGS84	BB57698	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57698_Upin_Bot2011_COI	S1-10	1	BB57698_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57698	Sample	BB57698	targeted	Absent	Sample	Upin_Bot2011_COI	S1-10	COI	45	BB57698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.49724
BB57699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83652	WGS84	BB57699	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57699_Cjap_Sim2018_COI	S1-11	1	BB57699_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57699	Sample	BB57699	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-11	COI	45	BB57699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50885
BB57699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83652	WGS84	BB57699	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	38.972	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57699_Mgig_Bot2012_COI	S1-11	1	BB57699_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57699	Sample	BB57699	targeted	Present	Sample	Mgig_Bot2012_COI	S1-11	COI	45	BB57699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.50885
BB57700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83672	WGS84	BB57700	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57700_Cmae_Bot2011_COI	S1-13	1	BB57700_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57700	Sample	BB57700	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-13	COI	45	BB57700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-13	ATGAACAGTCTATCCTCCTTTAG	138.50894
BB57702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80916	WGS84	BB57702	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57702_Aamu_Bax2006_COI	S1-16	1	BB57702_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57702	Sample	BB57702	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-16	COI	45	BB57702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-16	GCACAACCGGGATCTTTACTTC	138.51899
BB57702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80916	WGS84	BB57702	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57702_Pcan_Bot2011_IGS	S1-16	1	BB57702_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57702	Sample	BB57702	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-16	IGS	45	BB57702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-16	CGTAATCCTCAGTACTGGCTA	138.51899
BB57702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80916	WGS84	BB57702	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57702_Sspa_Oph2007_COI	S1-16	1	BB57702_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57702	Sample	BB57702	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-16	COI	45	BB57702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-16	GAAGGCCTTCCGATGATC	138.51899
BB57702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80916	WGS84	BB57702	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57702_Upin_Bot2011_COI	S1-16	1	BB57702_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57702	Sample	BB57702	targeted	Absent	Sample	Upin_Bot2011_COI	S1-16	COI	45	BB57702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.51899
BB57703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81017	WGS84	BB57703	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57703_Cjap_Sim2018_COI	S1-18	1	BB57703_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57703	Sample	BB57703	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-18	COI	45	BB57703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52224
BB57703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.81017	WGS84	BB57703	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.704	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57703_Mgig_Bot2012_COI	S1-18	1	BB57703_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57703	Sample	BB57703	targeted	Present	Sample	Mgig_Bot2012_COI	S1-18	COI	45	BB57703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.52224
BB57704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82663	WGS84	BB57704	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.983	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57704_Cmae_Bot2011_COI	T2-05	1	BB57704_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57704	Sample	BB57704	targeted	Present	Sample	Cmae_Bot2011_COI	T2-05	COI	45	BB57704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-05	ATGAACAGTCTATCCTCCTTTAG	147.3027
BB57706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88618	WGS84	BB57706	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57706_Aamu_Bax2006_COI	T2-06	1	BB57706_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57706	Sample	BB57706	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-06	COI	45	BB57706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-06	GCACAACCGGGATCTTTACTTC	147.33863
BB57706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88618	WGS84	BB57706	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57706_Pcan_Bot2011_IGS	T2-06	1	BB57706_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57706	Sample	BB57706	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-06	IGS	45	BB57706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-06	CGTAATCCTCAGTACTGGCTA	147.33863
BB57706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88618	WGS84	BB57706	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57706_Sspa_Oph2007_COI	T2-06	1	BB57706_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57706	Sample	BB57706	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-06	COI	45	BB57706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-06	GAAGGCCTTCCGATGATC	147.33863
BB57706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88618	WGS84	BB57706	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57706_Upin_Bot2011_COI	T2-06	1	BB57706_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57706	Sample	BB57706	targeted	Absent	Sample	Upin_Bot2011_COI	T2-06	COI	45	BB57706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.33863
BB57707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88341	WGS84	BB57707	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57707_Cjap_Sim2018_COI	T2-14	1	BB57707_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57707	Sample	BB57707	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-14	COI	45	BB57707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33708
BB57707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88341	WGS84	BB57707	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.106	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57707_Mgig_Bot2012_COI	T2-14	1	BB57707_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57707	Sample	BB57707	targeted	Present	Sample	Mgig_Bot2012_COI	T2-14	COI	45	BB57707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.33708
BB57708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9596	WGS84	BB57708	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57708_Cmae_Bot2011_COI	T2-07	1	BB57708_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57708	Sample	BB57708	targeted	Absent	Sample	Cmae_Bot2011_COI	T2-07	COI	45	BB57708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-07	ATGAACAGTCTATCCTCCTTTAG	147.52467
BB57710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85216	WGS84	BB57710	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57710_Cjap_Sim2018_COI	T2-08	1	BB57710_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57710	Sample	BB57710	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-08	COI	45	BB57710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87352
BB57710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85216	WGS84	BB57710	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.503	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	26/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57710_Mgig_Bot2012_COI	T2-08	1	BB57710_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57710	Sample	BB57710	targeted	Present	Sample	Mgig_Bot2012_COI	T2-08	COI	45	BB57710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.87352
BB57711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95936	WGS84	BB57711	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57711_Cmae_Bot2011_COI	T3-05	1	BB57711_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57711	Sample	BB57711	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-05	COI	45	BB57711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-05	ATGAACAGTCTATCCTCCTTTAG	147.52541
BB57713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82503	WGS84	BB57713	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57713_Aamu_Bax2006_COI	T2-09	1	BB57713_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57713	Sample	BB57713	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-09	COI	45	BB57713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-09	GCACAACCGGGATCTTTACTTC	147.30534
BB57713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82503	WGS84	BB57713	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57713_Pcan_Bot2011_IGS	T2-09	1	BB57713_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57713	Sample	BB57713	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-09	IGS	45	BB57713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-09	CGTAATCCTCAGTACTGGCTA	147.30534
BB57713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82503	WGS84	BB57713	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57713_Sspa_Oph2007_COI	T2-09	1	BB57713_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57713	Sample	BB57713	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-09	COI	45	BB57713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-09	GAAGGCCTTCCGATGATC	147.30534
BB57713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82503	WGS84	BB57713	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57713_Upin_Bot2011_COI	T2-09	1	BB57713_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57713	Sample	BB57713	targeted	Absent	Sample	Upin_Bot2011_COI	T2-09	COI	45	BB57713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.30534
BB57714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87762	WGS84	BB57714	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57714_Cjap_Sim2018_COI	T2-15	1	BB57714_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57714	Sample	BB57714	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-15	COI	45	BB57714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34147
BB57714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87762	WGS84	BB57714	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.715	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57714_Mgig_Bot2012_COI	T2-15	1	BB57714_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57714	Sample	BB57714	targeted	Present	Sample	Mgig_Bot2012_COI	T2-15	COI	45	BB57714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.34147
BB57715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88566	WGS84	BB57715	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57715_Cmae_Bot2011_COI	T2-02	1	BB57715_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57715	Sample	BB57715	targeted	Absent	Sample	Cmae_Bot2011_COI	T2-02	COI	45	BB57715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-02	ATGAACAGTCTATCCTCCTTTAG	147.33452
BB57717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88259	WGS84	BB57717	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57717_Aamu_Bax2006_COI	T2-16	1	BB57717_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57717	Sample	BB57717	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-16	COI	45	BB57717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-16	GCACAACCGGGATCTTTACTTC	147.33612
BB57717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88259	WGS84	BB57717	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57717_Pcan_Bot2011_IGS	T2-16	1	BB57717_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57717	Sample	BB57717	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-16	IGS	45	BB57717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-16	CGTAATCCTCAGTACTGGCTA	147.33612
BB57717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88259	WGS84	BB57717	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57717_Sspa_Oph2007_COI	T2-16	1	BB57717_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57717	Sample	BB57717	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-16	COI	45	BB57717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-16	GAAGGCCTTCCGATGATC	147.33612
BB57717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88259	WGS84	BB57717	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57717_Upin_Bot2011_COI	T2-16	1	BB57717_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57717	Sample	BB57717	targeted	Absent	Sample	Upin_Bot2011_COI	T2-16	COI	45	BB57717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.33612
BB57718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9601	WGS84	BB57718	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57718_Cjap_Sim2018_COI	T2-03	1	BB57718_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57718	Sample	BB57718	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-03	COI	45	BB57718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53037
BB57718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.9601	WGS84	BB57718	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.383	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	23/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57718_Mgig_Bot2012_COI	T2-03	1	BB57718_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57718	Sample	BB57718	targeted	Present	Sample	Mgig_Bot2012_COI	T2-03	COI	45	BB57718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.53037
BB57719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96959	WGS84	BB57719	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.638	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57719_Cmae_Bot2011_COI	T2-11	1	BB57719_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57719	Sample	BB57719	targeted	Present	Sample	Cmae_Bot2011_COI	T2-11	COI	45	BB57719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-11	ATGAACAGTCTATCCTCCTTTAG	147.51602
BB57720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84978	WGS84	BB57720	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57720_Aamu_Bax2006_COI	T3-06	1	BB57720_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57720	Sample	BB57720	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-06	COI	45	BB57720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-06	GCACAACCGGGATCTTTACTTC	147.8733
BB57720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84978	WGS84	BB57720	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57720_Pcan_Bot2011_IGS	T3-06	1	BB57720_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57720	Sample	BB57720	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-06	IGS	45	BB57720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-06	CGTAATCCTCAGTACTGGCTA	147.8733
BB57720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84978	WGS84	BB57720	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57720_Sspa_Oph2007_COI	T3-06	1	BB57720_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57720	Sample	BB57720	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-06	COI	45	BB57720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-06	GAAGGCCTTCCGATGATC	147.8733
BB57720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84978	WGS84	BB57720	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57720_Upin_Bot2011_COI	T3-06	1	BB57720_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57720	Sample	BB57720	targeted	Absent	Sample	Upin_Bot2011_COI	T3-06	COI	45	BB57720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.8733
BB57721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84817	WGS84	BB57721	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57721_Cjap_Sim2018_COI	T2-04	1	BB57721_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57721	Sample	BB57721	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-04	COI	45	BB57721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.8728
BB57721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.84817	WGS84	BB57721	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.127	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	26/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57721_Mgig_Bot2012_COI	T2-04	1	BB57721_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57721	Sample	BB57721	targeted	Present	Sample	Mgig_Bot2012_COI	T2-04	COI	45	BB57721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.8728
BB57722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85306	WGS84	BB57722	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57722_Cmae_Bot2011_COI	T2-12	1	BB57722_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57722	Sample	BB57722	targeted	Absent	Sample	Cmae_Bot2011_COI	T2-12	COI	45	BB57722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-12	ATGAACAGTCTATCCTCCTTTAG	147.87211
BB57724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84683	WGS84	BB57724	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57724_Aamu_Bax2006_COI	S2-01	1	BB57724_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57724	Sample	BB57724	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-01	COI	45	BB57724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-01	GCACAACCGGGATCTTTACTTC	138.49744
BB57724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84683	WGS84	BB57724	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57724_Pcan_Bot2011_IGS	S2-01	1	BB57724_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57724	Sample	BB57724	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-01	IGS	45	BB57724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-01	CGTAATCCTCAGTACTGGCTA	138.49744
BB57724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84683	WGS84	BB57724	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.801	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57724_Sspa_Oph2007_COI	S2-01	1	BB57724_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57724	Sample	BB57724	targeted	Present	Sample	Sspa_Oph2007_COI	S2-01	COI	45	BB57724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-01	GAAGGCCTTCCGATGATC	138.49744
BB57724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84683	WGS84	BB57724	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57724_Upin_Bot2011_COI	S2-01	1	BB57724_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57724	Sample	BB57724	targeted	Absent	Sample	Upin_Bot2011_COI	S2-01	COI	45	BB57724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.49744
BB57725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83693	WGS84	BB57725	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57725_Cjap_Sim2018_COI	S2-06	1	BB57725_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57725	Sample	BB57725	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-06	COI	45	BB57725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50748
BB57725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83693	WGS84	BB57725	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57725_Mgig_Bot2012_COI	S2-06	1	BB57725_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57725	Sample	BB57725	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-06	COI	45	BB57725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.50748
BB57726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7866	WGS84	BB57726	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57726_Cmae_Bot2011_COI	S2-12	1	BB57726_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57726	Sample	BB57726	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-12	COI	45	BB57726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-12	ATGAACAGTCTATCCTCCTTTAG	138.51435
BB57728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83658	WGS84	BB57728	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57728_Aamu_Bax2006_COI	S2-07	1	BB57728_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57728	Sample	BB57728	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-07	COI	45	BB57728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-07	GCACAACCGGGATCTTTACTTC	138.50823
BB57728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83658	WGS84	BB57728	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57728_Pcan_Bot2011_IGS	S2-07	1	BB57728_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57728	Sample	BB57728	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-07	IGS	45	BB57728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-07	CGTAATCCTCAGTACTGGCTA	138.50823
BB57728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83658	WGS84	BB57728	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.158	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57728_Sspa_Oph2007_COI	S2-07	1	BB57728_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57728	Sample	BB57728	targeted	Present	Sample	Sspa_Oph2007_COI	S2-07	COI	45	BB57728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-07	GAAGGCCTTCCGATGATC	138.50823
BB57728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83658	WGS84	BB57728	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57728_Upin_Bot2011_COI	S2-07	1	BB57728_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57728	Sample	BB57728	targeted	Absent	Sample	Upin_Bot2011_COI	S2-07	COI	45	BB57728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.50823
BB57729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84482	WGS84	BB57729	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57729_Cjap_Sim2018_COI	S2-04	1	BB57729_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57729	Sample	BB57729	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-04	COI	45	BB57729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49676
BB57729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84482	WGS84	BB57729	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57729_Mgig_Bot2012_COI	S2-04	1	BB57729_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57729	Sample	BB57729	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-04	COI	45	BB57729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49676
BB57731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82563	WGS84	BB57731	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57731_Aamu_Bax2006_COI	T3-09	1	BB57731_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57731	Sample	BB57731	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-09	COI	45	BB57731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-09	GCACAACCGGGATCTTTACTTC	147.30458
BB57731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82563	WGS84	BB57731	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57731_Pcan_Bot2011_IGS	T3-09	1	BB57731_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57731	Sample	BB57731	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-09	IGS	45	BB57731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-09	CGTAATCCTCAGTACTGGCTA	147.30458
BB57731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82563	WGS84	BB57731	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57731_Sspa_Oph2007_COI	T3-09	1	BB57731_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57731	Sample	BB57731	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-09	COI	45	BB57731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-09	GAAGGCCTTCCGATGATC	147.30458
BB57731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82563	WGS84	BB57731	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57731_Upin_Bot2011_COI	T3-09	1	BB57731_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57731	Sample	BB57731	targeted	Absent	Sample	Upin_Bot2011_COI	T3-09	COI	45	BB57731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.30458
BB57732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85213	WGS84	BB57732	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57732_Cjap_Sim2018_COI	T3-07	1	BB57732_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57732	Sample	BB57732	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-07	COI	45	BB57732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87312
BB57732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85213	WGS84	BB57732	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.767	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	4/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57732_Mgig_Bot2012_COI	T3-07	1	BB57732_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57732	Sample	BB57732	targeted	Present	Sample	Mgig_Bot2012_COI	T3-07	COI	45	BB57732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.87312
BB57733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82744	WGS84	BB57733	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57733_Cmae_Bot2011_COI	T3-08	1	BB57733_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57733	Sample	BB57733	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-08	COI	45	BB57733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-08	ATGAACAGTCTATCCTCCTTTAG	147.30218
BB57735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88567	WGS84	BB57735	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57735_Aamu_Bax2006_COI	T3-02	1	BB57735_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57735	Sample	BB57735	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-02	COI	45	BB57735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-02	GCACAACCGGGATCTTTACTTC	147.33476
BB57735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88567	WGS84	BB57735	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57735_Pcan_Bot2011_IGS	T3-02	1	BB57735_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57735	Sample	BB57735	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-02	IGS	45	BB57735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-02	CGTAATCCTCAGTACTGGCTA	147.33476
BB57735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88567	WGS84	BB57735	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57735_Sspa_Oph2007_COI	T3-02	1	BB57735_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57735	Sample	BB57735	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-02	COI	45	BB57735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-02	GAAGGCCTTCCGATGATC	147.33476
BB57735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88567	WGS84	BB57735	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57735_Upin_Bot2011_COI	T3-02	1	BB57735_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57735	Sample	BB57735	targeted	Absent	Sample	Upin_Bot2011_COI	T3-02	COI	45	BB57735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.33476
BB57736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82869	WGS84	BB57736	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57736_Cjap_Sim2018_COI	T3-01	1	BB57736_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57736	Sample	BB57736	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-01	COI	45	BB57736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30215
BB57736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82869	WGS84	BB57736	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.124	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57736_Mgig_Bot2012_COI	T3-01	1	BB57736_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57736	Sample	BB57736	targeted	Present	Sample	Mgig_Bot2012_COI	T3-01	COI	45	BB57736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.30215
BB57737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88386	WGS84	BB57737	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.873	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57737_Cmae_Bot2011_COI	T3-11	1	BB57737_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57737	Sample	BB57737	targeted	Present	Sample	Cmae_Bot2011_COI	T3-11	COI	45	BB57737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-11	ATGAACAGTCTATCCTCCTTTAG	147.33667
BB57740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96226	WGS84	BB57740	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57740_Cmae_Bot2011_COI	T3-10	1	BB57740_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57740	Sample	BB57740	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-10	COI	45	BB57740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-10	ATGAACAGTCTATCCTCCTTTAG	147.51947
BB57742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88955	WGS84	BB57742	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57742_Aamu_Bax2006_COI	T3-13	1	BB57742_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57742	Sample	BB57742	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-13	COI	45	BB57742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-13	GCACAACCGGGATCTTTACTTC	147.81318
BB57742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88955	WGS84	BB57742	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57742_Pcan_Bot2011_IGS	T3-13	1	BB57742_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57742	Sample	BB57742	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-13	IGS	45	BB57742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-13	CGTAATCCTCAGTACTGGCTA	147.81318
BB57742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88955	WGS84	BB57742	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57742_Sspa_Oph2007_COI	T3-13	1	BB57742_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57742	Sample	BB57742	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-13	COI	45	BB57742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-13	GAAGGCCTTCCGATGATC	147.81318
BB57742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88955	WGS84	BB57742	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57742_Upin_Bot2011_COI	T3-13	1	BB57742_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57742	Sample	BB57742	targeted	Absent	Sample	Upin_Bot2011_COI	T3-13	COI	45	BB57742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.81318
BB57743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88241	WGS84	BB57743	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57743_Cjap_Sim2018_COI	T3-15	1	BB57743_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57743	Sample	BB57743	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-15	COI	45	BB57743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33604
BB57743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88241	WGS84	BB57743	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57743_Mgig_Bot2012_COI	T3-15	1	BB57743_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57743	Sample	BB57743	targeted	Absent	Sample	Mgig_Bot2012_COI	T3-15	COI	45	BB57743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.33604
BB57744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87892	WGS84	BB57744	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57744_Cmae_Bot2011_COI	T3-14	1	BB57744_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57744	Sample	BB57744	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-14	COI	45	BB57744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-14	ATGAACAGTCTATCCTCCTTTAG	147.34139
BB73902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82086	WGS84	BB73902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.372	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73902_Aamu_Bax2006_COI	MA49	1	BB73902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73902	Sample	BB73902	targeted	Present	Sample	Aamu_Bax2006_COI	MA49	COI	45	BB73902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA49	GCACAACCGGGATCTTTACTTC	144.91451
BB73902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82086	WGS84	BB73902	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73902_Pcan_Bot2011_IGS	MA49	1	BB73902_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73902	Sample	BB73902	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA49	IGS	45	BB73902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA49	CGTAATCCTCAGTACTGGCTA	144.91451
BB73902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82086	WGS84	BB73902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.98	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73902_Sspa_Oph2007_COI	MA49	1	BB73902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73902	Sample	BB73902	targeted	Present	Sample	Sspa_Oph2007_COI	MA49	COI	45	BB73902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA49	GAAGGCCTTCCGATGATC	144.91451
BB73902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82086	WGS84	BB73902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73902_Upin_Bot2011_COI	MA49	1	BB73902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73902	Sample	BB73902	targeted	Absent	Sample	Upin_Bot2011_COI	MA49	COI	45	BB73902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA49	CTTTAATTACAGCGTTTTTATTGTTGT	144.91451
BB73903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81657	WGS84	BB73903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73903_Mgig_Bot2012_COI	MA48	1	BB73903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73903	Sample	BB73903	targeted	Absent	Sample	Mgig_Bot2012_COI	MA48	COI	45	BB73903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA48	TCTTATTCGTTGGAGACTTTATAACCCT	144.92576
BB73904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82918	WGS84	BB73904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73904_Cmae_Bot2011_COI	MA47	1	BB73904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73904	Sample	BB73904	targeted	Absent	Sample	Cmae_Bot2011_COI	MA47	COI	45	BB73904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA47	ATGAACAGTCTATCCTCCTTTAG	144.90038
BB73904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82918	WGS84	BB73904	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73904_Msal_Bot2012_COI	MA47	1	BB73904_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73904	Sample	BB73904	targeted	Absent	Sample	Msal_Bot2012_COI	MA47	COI	45	BB73904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA47	GYTAGTTCCRATGATGTTAGCTG	144.90038
BB73906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82042	WGS84	BB73906	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.153	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73906_Aamu_Bax2006_COI	MA17	1	BB73906_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73906	Sample	BB73906	targeted	Present	Sample	Aamu_Bax2006_COI	MA17	COI	45	BB73906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA17	GCACAACCGGGATCTTTACTTC	144.91668
BB73906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82042	WGS84	BB73906	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73906_Pcan_Bot2011_IGS	MA17	1	BB73906_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73906	Sample	BB73906	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA17	IGS	45	BB73906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA17	CGTAATCCTCAGTACTGGCTA	144.91668
BB73906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82042	WGS84	BB73906	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73906_Sspa_Oph2007_COI	MA17	1	BB73906_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73906	Sample	BB73906	targeted	Absent	Sample	Sspa_Oph2007_COI	MA17	COI	45	BB73906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA17	GAAGGCCTTCCGATGATC	144.91668
BB73906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82042	WGS84	BB73906	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73906_Upin_Bot2011_COI	MA17	1	BB73906_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73906	Sample	BB73906	targeted	Absent	Sample	Upin_Bot2011_COI	MA17	COI	45	BB73906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA17	CTTTAATTACAGCGTTTTTATTGTTGT	144.91668
BB73907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81685	WGS84	BB73907	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73907_Mgig_Bot2012_COI	MA7	1	BB73907_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73907	Sample	BB73907	targeted	Absent	Sample	Mgig_Bot2012_COI	MA7	COI	45	BB73907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA7	TCTTATTCGTTGGAGACTTTATAACCCT	144.92339
BB73908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81914	WGS84	BB73908	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73908_Cmae_Bot2011_COI	MA6	1	BB73908_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73908	Sample	BB73908	targeted	Absent	Sample	Cmae_Bot2011_COI	MA6	COI	45	BB73908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA6	ATGAACAGTCTATCCTCCTTTAG	144.92582
BB73908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81914	WGS84	BB73908	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73908_Msal_Bot2012_COI	MA6	1	BB73908_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73908	Sample	BB73908	targeted	Absent	Sample	Msal_Bot2012_COI	MA6	COI	45	BB73908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA6	GYTAGTTCCRATGATGTTAGCTG	144.92582
BB73910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44688	WGS84	BB73910	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73910_Mgig_Bot2012_COI	BA11	1	BB73910_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73910	Sample	BB73910	targeted	Absent	Sample	Mgig_Bot2012_COI	BA11	COI	45	BB73910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA11	TCTTATTCGTTGGAGACTTTATAACCCT	153.07904
BB73911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB73911	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73911_Cmae_Bot2011_COI	MA2	1	BB73911_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73911	Sample	BB73911	targeted	Absent	Sample	Cmae_Bot2011_COI	MA2	COI	45	BB73911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA2	ATGAACAGTCTATCCTCCTTTAG	144.93107
BB73911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB73911	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73911_Msal_Bot2012_COI	MA2	1	BB73911_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73911	Sample	BB73911	targeted	Absent	Sample	Msal_Bot2012_COI	MA2	COI	45	BB73911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA2	GYTAGTTCCRATGATGTTAGCTG	144.93107
BB73913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81128	WGS84	BB73913	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	20.871	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73913_Aamu_Bax2006_COI	GA110	1	BB73913_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73913	Sample	BB73913	targeted	Present	Sample	Aamu_Bax2006_COI	GA110	COI	45	BB73913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA110	GCACAACCGGGATCTTTACTTC	144.91423
BB73913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81128	WGS84	BB73913	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73913_Pcan_Bot2011_IGS	GA110	1	BB73913_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73913	Sample	BB73913	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA110	IGS	45	BB73913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA110	CGTAATCCTCAGTACTGGCTA	144.91423
BB73913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81128	WGS84	BB73913	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.367	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73913_Sspa_Oph2007_COI	GA110	1	BB73913_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73913	Sample	BB73913	targeted	Present	Sample	Sspa_Oph2007_COI	GA110	COI	45	BB73913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA110	GAAGGCCTTCCGATGATC	144.91423
BB73913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81128	WGS84	BB73913	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.852	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73913_Upin_Bot2011_COI	GA110	1	BB73913_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73913	Sample	BB73913	targeted	Present	Sample	Upin_Bot2011_COI	GA110	COI	45	BB73913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA110	CTTTAATTACAGCGTTTTTATTGTTGT	144.91423
BB73914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44441	WGS84	BB73914	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73914_Mgig_Bot2012_COI	BA12	1	BB73914_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73914	Sample	BB73914	targeted	Absent	Sample	Mgig_Bot2012_COI	BA12	COI	45	BB73914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA12	TCTTATTCGTTGGAGACTTTATAACCCT	153.07844
BB73915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82059	WGS84	BB73915	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73915_Cmae_Bot2011_COI	GA100	1	BB73915_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73915	Sample	BB73915	targeted	Absent	Sample	Cmae_Bot2011_COI	GA100	COI	45	BB73915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA100	ATGAACAGTCTATCCTCCTTTAG	144.90597
BB73915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82059	WGS84	BB73915	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73915_Msal_Bot2012_COI	GA100	1	BB73915_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73915	Sample	BB73915	targeted	Absent	Sample	Msal_Bot2012_COI	GA100	COI	45	BB73915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA100	GYTAGTTCCRATGATGTTAGCTG	144.90597
BB73917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81858	WGS84	BB73917	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	19.726	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73917_Aamu_Bax2006_COI	GA90	1	BB73917_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73917	Sample	BB73917	targeted	Present	Sample	Aamu_Bax2006_COI	GA90	COI	45	BB73917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA90	GCACAACCGGGATCTTTACTTC	144.9152
BB73917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81858	WGS84	BB73917	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73917_Pcan_Bot2011_IGS	GA90	1	BB73917_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73917	Sample	BB73917	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA90	IGS	45	BB73917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA90	CGTAATCCTCAGTACTGGCTA	144.9152
BB73917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81858	WGS84	BB73917	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.673	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73917_Sspa_Oph2007_COI	GA90	1	BB73917_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73917	Sample	BB73917	targeted	Present	Sample	Sspa_Oph2007_COI	GA90	COI	45	BB73917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA90	GAAGGCCTTCCGATGATC	144.9152
BB73917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81858	WGS84	BB73917	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73917_Upin_Bot2011_COI	GA90	1	BB73917_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73917	Sample	BB73917	targeted	Absent	Sample	Upin_Bot2011_COI	GA90	COI	45	BB73917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA90	CTTTAATTACAGCGTTTTTATTGTTGT	144.9152
BB73918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81632	WGS84	BB73918	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73918_Mgig_Bot2012_COI	GA85	1	BB73918_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73918	Sample	BB73918	targeted	Absent	Sample	Mgig_Bot2012_COI	GA85	COI	45	BB73918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA85	TCTTATTCGTTGGAGACTTTATAACCCT	144.91413
BB73919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81129	WGS84	BB73919	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73919_Cmae_Bot2011_COI	GA80	1	BB73919_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73919	Sample	BB73919	targeted	Absent	Sample	Cmae_Bot2011_COI	GA80	COI	45	BB73919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA80	ATGAACAGTCTATCCTCCTTTAG	144.91354
BB73919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81129	WGS84	BB73919	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73919_Msal_Bot2012_COI	GA80	1	BB73919_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73919	Sample	BB73919	targeted	Absent	Sample	Msal_Bot2012_COI	GA80	COI	45	BB73919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA80	GYTAGTTCCRATGATGTTAGCTG	144.91354
BB73920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81899	WGS84	BB73920	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.72	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73920_Aamu_Bax2006_COI	GA75	1	BB73920_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73920	Sample	BB73920	targeted	Present	Sample	Aamu_Bax2006_COI	GA75	COI	45	BB73920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA75	GCACAACCGGGATCTTTACTTC	144.91136
BB73920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81899	WGS84	BB73920	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73920_Pcan_Bot2011_IGS	GA75	1	BB73920_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73920	Sample	BB73920	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA75	IGS	45	BB73920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA75	CGTAATCCTCAGTACTGGCTA	144.91136
BB73920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81899	WGS84	BB73920	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73920_Sspa_Oph2007_COI	GA75	1	BB73920_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73920	Sample	BB73920	targeted	Absent	Sample	Sspa_Oph2007_COI	GA75	COI	45	BB73920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA75	GAAGGCCTTCCGATGATC	144.91136
BB73920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81899	WGS84	BB73920	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73920_Upin_Bot2011_COI	GA75	1	BB73920_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73920	Sample	BB73920	targeted	Absent	Sample	Upin_Bot2011_COI	GA75	COI	45	BB73920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA75	CTTTAATTACAGCGTTTTTATTGTTGT	144.91136
BB73921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82095	WGS84	BB73921	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73921_Mgig_Bot2012_COI	GA70	1	BB73921_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73921	Sample	BB73921	targeted	Absent	Sample	Mgig_Bot2012_COI	GA70	COI	45	BB73921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA70	TCTTATTCGTTGGAGACTTTATAACCCT	144.90757
BB73922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82287	WGS84	BB73922	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.91	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73922_Cmae_Bot2011_COI	GA65	1	BB73922_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73922	Sample	BB73922	targeted	Present	Sample	Cmae_Bot2011_COI	GA65	COI	45	BB73922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA65	ATGAACAGTCTATCCTCCTTTAG	144.90529
BB73922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82287	WGS84	BB73922	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73922_Msal_Bot2012_COI	GA65	1	BB73922_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73922	Sample	BB73922	targeted	Absent	Sample	Msal_Bot2012_COI	GA65	COI	45	BB73922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA65	GYTAGTTCCRATGATGTTAGCTG	144.90529
BB73924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81631	WGS84	BB73924	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	19.883	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73924_Aamu_Bax2006_COI	GA50	1	BB73924_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73924	Sample	BB73924	targeted	Present	Sample	Aamu_Bax2006_COI	GA50	COI	45	BB73924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA50	GCACAACCGGGATCTTTACTTC	144.91317
BB73924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81631	WGS84	BB73924	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73924_Pcan_Bot2011_IGS	GA50	1	BB73924_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73924	Sample	BB73924	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA50	IGS	45	BB73924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA50	CGTAATCCTCAGTACTGGCTA	144.91317
BB73924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81631	WGS84	BB73924	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.643	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73924_Sspa_Oph2007_COI	GA50	1	BB73924_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73924	Sample	BB73924	targeted	Present	Sample	Sspa_Oph2007_COI	GA50	COI	45	BB73924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA50	GAAGGCCTTCCGATGATC	144.91317
BB73924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81631	WGS84	BB73924	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73924_Upin_Bot2011_COI	GA50	1	BB73924_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73924	Sample	BB73924	targeted	Absent	Sample	Upin_Bot2011_COI	GA50	COI	45	BB73924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA50	CTTTAATTACAGCGTTTTTATTGTTGT	144.91317
BB73925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81443	WGS84	BB73925	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73925_Mgig_Bot2012_COI	GA45	1	BB73925_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73925	Sample	BB73925	targeted	Absent	Sample	Mgig_Bot2012_COI	GA45	COI	45	BB73925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA45	TCTTATTCGTTGGAGACTTTATAACCCT	144.91251
BB73926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44611	WGS84	BB73926	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73926_Cmae_Bot2011_COI	BA14	1	BB73926_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73926	Sample	BB73926	targeted	Absent	Sample	Cmae_Bot2011_COI	BA14	COI	45	BB73926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA14	ATGAACAGTCTATCCTCCTTTAG	153.08337
BB73926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44611	WGS84	BB73926	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73926_Msal_Bot2012_COI	BA14	1	BB73926_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73926	Sample	BB73926	targeted	Absent	Sample	Msal_Bot2012_COI	BA14	COI	45	BB73926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA14	GYTAGTTCCRATGATGTTAGCTG	153.08337
BB73928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81867	WGS84	BB73928	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.703	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73928_Aamu_Bax2006_COI	GA30	1	BB73928_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73928	Sample	BB73928	targeted	Present	Sample	Aamu_Bax2006_COI	GA30	COI	45	BB73928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA30	GCACAACCGGGATCTTTACTTC	144.91782
BB73928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81867	WGS84	BB73928	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73928_Pcan_Bot2011_IGS	GA30	1	BB73928_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73928	Sample	BB73928	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA30	IGS	45	BB73928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA30	CGTAATCCTCAGTACTGGCTA	144.91782
BB73928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81867	WGS84	BB73928	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.981	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73928_Sspa_Oph2007_COI	GA30	1	BB73928_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73928	Sample	BB73928	targeted	Present	Sample	Sspa_Oph2007_COI	GA30	COI	45	BB73928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA30	GAAGGCCTTCCGATGATC	144.91782
BB73928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81867	WGS84	BB73928	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73928_Upin_Bot2011_COI	GA30	1	BB73928_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73928	Sample	BB73928	targeted	Absent	Sample	Upin_Bot2011_COI	GA30	COI	45	BB73928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA30	CTTTAATTACAGCGTTTTTATTGTTGT	144.91782
BB73929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81279	WGS84	BB73929	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73929_Mgig_Bot2012_COI	GA25	1	BB73929_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73929	Sample	BB73929	targeted	Absent	Sample	Mgig_Bot2012_COI	GA25	COI	45	BB73929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA25	TCTTATTCGTTGGAGACTTTATAACCCT	144.91442
BB73931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44806	WGS84	BB73931	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73931_Aamu_Bax2006_COI	BA17	1	BB73931_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73931	Sample	BB73931	targeted	Absent	Sample	Aamu_Bax2006_COI	BA17	COI	45	BB73931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA17	GCACAACCGGGATCTTTACTTC	153.094
BB73931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44806	WGS84	BB73931	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73931_Pcan_Bot2011_IGS	BA17	1	BB73931_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73931	Sample	BB73931	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA17	IGS	45	BB73931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA17	CGTAATCCTCAGTACTGGCTA	153.094
BB73931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44806	WGS84	BB73931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73931_Sspa_Oph2007_COI	BA17	1	BB73931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73931	Sample	BB73931	targeted	Absent	Sample	Sspa_Oph2007_COI	BA17	COI	45	BB73931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA17	GAAGGCCTTCCGATGATC	153.094
BB73931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44806	WGS84	BB73931	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73931_Upin_Bot2011_COI	BA17	1	BB73931_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73931	Sample	BB73931	targeted	Absent	Sample	Upin_Bot2011_COI	BA17	COI	45	BB73931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA17	CTTTAATTACAGCGTTTTTATTGTTGT	153.094
BB73932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82371	WGS84	BB73932	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73932_Mgig_Bot2012_COI	GA10	1	BB73932_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73932	Sample	BB73932	targeted	Absent	Sample	Mgig_Bot2012_COI	GA10	COI	45	BB73932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA10	TCTTATTCGTTGGAGACTTTATAACCCT	144.90329
BB73933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43303	WGS84	BB73933	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73933_Cmae_Bot2011_COI	BA18	1	BB73933_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73933	Sample	BB73933	targeted	Absent	Sample	Cmae_Bot2011_COI	BA18	COI	45	BB73933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA18	ATGAACAGTCTATCCTCCTTTAG	153.11629
BB73933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43303	WGS84	BB73933	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73933_Msal_Bot2012_COI	BA18	1	BB73933_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73933	Sample	BB73933	targeted	Absent	Sample	Msal_Bot2012_COI	BA18	COI	45	BB73933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA18	GYTAGTTCCRATGATGTTAGCTG	153.11629
BB73935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85877	WGS84	BB73935	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.079	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73935_Aamu_Bax2006_COI	MA119	1	BB73935_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73935	Sample	BB73935	targeted	Present	Sample	Aamu_Bax2006_COI	MA119	COI	45	BB73935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA119	GCACAACCGGGATCTTTACTTC	144.91141
BB73935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85877	WGS84	BB73935	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73935_Pcan_Bot2011_IGS	MA119	1	BB73935_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73935	Sample	BB73935	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA119	IGS	45	BB73935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA119	CGTAATCCTCAGTACTGGCTA	144.91141
BB73935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85877	WGS84	BB73935	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.745	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73935_Sspa_Oph2007_COI	MA119	1	BB73935_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73935	Sample	BB73935	targeted	Present	Sample	Sspa_Oph2007_COI	MA119	COI	45	BB73935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA119	GAAGGCCTTCCGATGATC	144.91141
BB73935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85877	WGS84	BB73935	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.865	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73935_Upin_Bot2011_COI	MA119	1	BB73935_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73935	Sample	BB73935	targeted	Present	Sample	Upin_Bot2011_COI	MA119	COI	45	BB73935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA119	CTTTAATTACAGCGTTTTTATTGTTGT	144.91141
BB73936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3596	WGS84	BB73936	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73936_Mgig_Bot2012_COI	BA22	1	BB73936_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73936	Sample	BB73936	targeted	Absent	Sample	Mgig_Bot2012_COI	BA22	COI	45	BB73936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA22	TCTTATTCGTTGGAGACTTTATAACCCT	153.17773
BB73937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86045	WGS84	BB73937	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73937_Cmae_Bot2011_COI	MA87	1	BB73937_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73937	Sample	BB73937	targeted	Absent	Sample	Cmae_Bot2011_COI	MA87	COI	45	BB73937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA87	ATGAACAGTCTATCCTCCTTTAG	144.91439
BB73937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86045	WGS84	BB73937	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73937_Msal_Bot2012_COI	MA87	1	BB73937_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73937	Sample	BB73937	targeted	Absent	Sample	Msal_Bot2012_COI	MA87	COI	45	BB73937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA87	GYTAGTTCCRATGATGTTAGCTG	144.91439
BB73939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36686	WGS84	BB73939	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73939_Aamu_Bax2006_COI	BA26	1	BB73939_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73939	Sample	BB73939	targeted	Absent	Sample	Aamu_Bax2006_COI	BA26	COI	45	BB73939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA26	GCACAACCGGGATCTTTACTTC	153.17334
BB73939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36686	WGS84	BB73939	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73939_Pcan_Bot2011_IGS	BA26	1	BB73939_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73939	Sample	BB73939	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA26	IGS	45	BB73939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA26	CGTAATCCTCAGTACTGGCTA	153.17334
BB73939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36686	WGS84	BB73939	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73939_Sspa_Oph2007_COI	BA26	1	BB73939_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73939	Sample	BB73939	targeted	Absent	Sample	Sspa_Oph2007_COI	BA26	COI	45	BB73939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA26	GAAGGCCTTCCGATGATC	153.17334
BB73939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36686	WGS84	BB73939	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73939_Upin_Bot2011_COI	BA26	1	BB73939_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73939	Sample	BB73939	targeted	Absent	Sample	Upin_Bot2011_COI	BA26	COI	45	BB73939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA26	CTTTAATTACAGCGTTTTTATTGTTGT	153.17334
BB73940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3702	WGS84	BB73940	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73940_Cmae_Bot2011_COI	BA28	1	BB73940_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73940	Sample	BB73940	targeted	Absent	Sample	Cmae_Bot2011_COI	BA28	COI	45	BB73940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA28	ATGAACAGTCTATCCTCCTTTAG	153.1708
BB73940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3702	WGS84	BB73940	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73940_Msal_Bot2012_COI	BA28	1	BB73940_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73940	Sample	BB73940	targeted	Absent	Sample	Msal_Bot2012_COI	BA28	COI	45	BB73940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA28	GYTAGTTCCRATGATGTTAGCTG	153.1708
BB73942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86185	WGS84	BB73942	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.784	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73942_Aamu_Bax2006_COI	MA79	1	BB73942_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73942	Sample	BB73942	targeted	Present	Sample	Aamu_Bax2006_COI	MA79	COI	45	BB73942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA79	GCACAACCGGGATCTTTACTTC	144.91458
BB73942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86185	WGS84	BB73942	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73942_Pcan_Bot2011_IGS	MA79	1	BB73942_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73942	Sample	BB73942	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA79	IGS	45	BB73942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA79	CGTAATCCTCAGTACTGGCTA	144.91458
BB73942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86185	WGS84	BB73942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.937	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73942_Sspa_Oph2007_COI	MA79	1	BB73942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73942	Sample	BB73942	targeted	Present	Sample	Sspa_Oph2007_COI	MA79	COI	45	BB73942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA79	GAAGGCCTTCCGATGATC	144.91458
BB73942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86185	WGS84	BB73942	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.729	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73942_Upin_Bot2011_COI	MA79	1	BB73942_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73942	Sample	BB73942	targeted	Present	Sample	Upin_Bot2011_COI	MA79	COI	45	BB73942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA79	CTTTAATTACAGCGTTTTTATTGTTGT	144.91458
BB73943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8606	WGS84	BB73943	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73943_Mgig_Bot2012_COI	MA78	1	BB73943_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73943	Sample	BB73943	targeted	Absent	Sample	Mgig_Bot2012_COI	MA78	COI	45	BB73943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA78	TCTTATTCGTTGGAGACTTTATAACCCT	144.91438
BB73944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86423	WGS84	BB73944	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73944_Cmae_Bot2011_COI	MA76	1	BB73944_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73944	Sample	BB73944	targeted	Absent	Sample	Cmae_Bot2011_COI	MA76	COI	45	BB73944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA76	ATGAACAGTCTATCCTCCTTTAG	144.91825
BB73944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86423	WGS84	BB73944	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73944_Msal_Bot2012_COI	MA76	1	BB73944_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73944	Sample	BB73944	targeted	Absent	Sample	Msal_Bot2012_COI	MA76	COI	45	BB73944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA76	GYTAGTTCCRATGATGTTAGCTG	144.91825
BB73946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37653	WGS84	BB73946	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73946_Aamu_Bax2006_COI	BA32	1	BB73946_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73946	Sample	BB73946	targeted	Absent	Sample	Aamu_Bax2006_COI	BA32	COI	45	BB73946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA32	GCACAACCGGGATCTTTACTTC	153.16689
BB73946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37653	WGS84	BB73946	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73946_Pcan_Bot2011_IGS	BA32	1	BB73946_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73946	Sample	BB73946	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA32	IGS	45	BB73946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA32	CGTAATCCTCAGTACTGGCTA	153.16689
BB73946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37653	WGS84	BB73946	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73946_Sspa_Oph2007_COI	BA32	1	BB73946_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73946	Sample	BB73946	targeted	Absent	Sample	Sspa_Oph2007_COI	BA32	COI	45	BB73946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA32	GAAGGCCTTCCGATGATC	153.16689
BB73946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37653	WGS84	BB73946	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73946_Upin_Bot2011_COI	BA32	1	BB73946_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73946	Sample	BB73946	targeted	Absent	Sample	Upin_Bot2011_COI	BA32	COI	45	BB73946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA32	CTTTAATTACAGCGTTTTTATTGTTGT	153.16689
BB73947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37905	WGS84	BB73947	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73947_Mgig_Bot2012_COI	BA34	1	BB73947_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73947	Sample	BB73947	targeted	Absent	Sample	Mgig_Bot2012_COI	BA34	COI	45	BB73947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA34	TCTTATTCGTTGGAGACTTTATAACCCT	153.16432
BB73948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38147	WGS84	BB73948	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73948_Cmae_Bot2011_COI	BA36	1	BB73948_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73948	Sample	BB73948	targeted	Absent	Sample	Cmae_Bot2011_COI	BA36	COI	45	BB73948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA36	ATGAACAGTCTATCCTCCTTTAG	153.16215
BB73948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38147	WGS84	BB73948	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73948_Msal_Bot2012_COI	BA36	1	BB73948_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73948	Sample	BB73948	targeted	Absent	Sample	Msal_Bot2012_COI	BA36	COI	45	BB73948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA36	GYTAGTTCCRATGATGTTAGCTG	153.16215
BB73950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38365	WGS84	BB73950	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73950_Mgig_Bot2012_COI	BA38	1	BB73950_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73950	Sample	BB73950	targeted	Absent	Sample	Mgig_Bot2012_COI	BA38	COI	45	BB73950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA38	TCTTATTCGTTGGAGACTTTATAACCCT	153.16045
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73951_Cmae_Bot2011_COI	MA19	1	BB73951_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Absent	Sample	Cmae_Bot2011_COI	MA19	COI	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	ATGAACAGTCTATCCTCCTTTAG	144.90637
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73951_Hsan_Wil2021_COI	MA19	1	BB73951_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Absent	Sample	Hsan_Wil2021_COI	MA19	COI	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	CCTGGGCCGGTATAGTAGGT	144.90637
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73951_Msal_Bot2012_COI	MA19	1	BB73951_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Absent	Sample	Msal_Bot2012_COI	MA19	COI	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	GYTAGTTCCRATGATGTTAGCTG	144.90637
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.137	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73953_Aamu_Bax2006_COI	MA16	1	BB73953_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Present	Sample	Aamu_Bax2006_COI	MA16	COI	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	GCACAACCGGGATCTTTACTTC	144.91541
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73953_Pcan_Bot2011_IGS	MA16	1	BB73953_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA16	IGS	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	CGTAATCCTCAGTACTGGCTA	144.91541
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.974	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73953_Sspa_Oph2007_COI	MA16	1	BB73953_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Present	Sample	Sspa_Oph2007_COI	MA16	COI	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	GAAGGCCTTCCGATGATC	144.91541
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.3	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73953_Upin_Bot2011_COI	MA16	1	BB73953_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Present	Sample	Upin_Bot2011_COI	MA16	COI	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	CTTTAATTACAGCGTTTTTATTGTTGT	144.91541
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73954_Mgig_Bot2012_COI	MA14	1	BB73954_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Absent	Sample	Mgig_Bot2012_COI	MA14	COI	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	TCTTATTCGTTGGAGACTTTATAACCCT	144.91044
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73955_Cmae_Bot2011_COI	MA13	1	BB73955_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Absent	Sample	Cmae_Bot2011_COI	MA13	COI	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	ATGAACAGTCTATCCTCCTTTAG	144.9161
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73955_Hsan_Wil2021_COI	MA13	1	BB73955_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Absent	Sample	Hsan_Wil2021_COI	MA13	COI	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	CCTGGGCCGGTATAGTAGGT	144.9161
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73955_Msal_Bot2012_COI	MA13	1	BB73955_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Absent	Sample	Msal_Bot2012_COI	MA13	COI	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	GYTAGTTCCRATGATGTTAGCTG	144.9161
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.065	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73957_Aamu_Bax2006_COI	MA11	1	BB73957_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Present	Sample	Aamu_Bax2006_COI	MA11	COI	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	GCACAACCGGGATCTTTACTTC	144.91348
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73957_Pcan_Bot2011_IGS	MA11	1	BB73957_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA11	IGS	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	CGTAATCCTCAGTACTGGCTA	144.91348
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.82	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73957_Sspa_Oph2007_COI	MA11	1	BB73957_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Present	Sample	Sspa_Oph2007_COI	MA11	COI	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	GAAGGCCTTCCGATGATC	144.91348
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.434	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73957_Upin_Bot2011_COI	MA11	1	BB73957_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Present	Sample	Upin_Bot2011_COI	MA11	COI	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	CTTTAATTACAGCGTTTTTATTGTTGT	144.91348
BB73958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38913	WGS84	BB73958	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73958_Mgig_Bot2012_COI	BA42	1	BB73958_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73958	Sample	BB73958	targeted	Absent	Sample	Mgig_Bot2012_COI	BA42	COI	45	BB73958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA42	TCTTATTCGTTGGAGACTTTATAACCCT	153.15793
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73959_Cmae_Bot2011_COI	MA8	1	BB73959_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Absent	Sample	Cmae_Bot2011_COI	MA8	COI	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	ATGAACAGTCTATCCTCCTTTAG	144.9099
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73959_Hsan_Wil2021_COI	MA8	1	BB73959_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Absent	Sample	Hsan_Wil2021_COI	MA8	COI	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	CCTGGGCCGGTATAGTAGGT	144.9099
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73959_Msal_Bot2012_COI	MA8	1	BB73959_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Absent	Sample	Msal_Bot2012_COI	MA8	COI	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	GYTAGTTCCRATGATGTTAGCTG	144.9099
BB73960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.39185	WGS84	BB73960	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73960_Aamu_Bax2006_COI	BA43	1	BB73960_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73960	Sample	BB73960	targeted	Absent	Sample	Aamu_Bax2006_COI	BA43	COI	45	BB73960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA43	GCACAACCGGGATCTTTACTTC	153.15692
BB73960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.39185	WGS84	BB73960	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73960_Pcan_Bot2011_IGS	BA43	1	BB73960_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73960	Sample	BB73960	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA43	IGS	45	BB73960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA43	CGTAATCCTCAGTACTGGCTA	153.15692
BB73960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.39185	WGS84	BB73960	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73960_Sspa_Oph2007_COI	BA43	1	BB73960_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73960	Sample	BB73960	targeted	Absent	Sample	Sspa_Oph2007_COI	BA43	COI	45	BB73960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA43	GAAGGCCTTCCGATGATC	153.15692
BB73960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.39185	WGS84	BB73960	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73960_Upin_Bot2011_COI	BA43	1	BB73960_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73960	Sample	BB73960	targeted	Absent	Sample	Upin_Bot2011_COI	BA43	COI	45	BB73960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA43	CTTTAATTACAGCGTTTTTATTGTTGT	153.15692
BB73961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44508	WGS84	BB73961	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73961_Mgig_Bot2012_COI	BA5	1	BB73961_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73961	Sample	BB73961	targeted	Absent	Sample	Mgig_Bot2012_COI	BA5	COI	45	BB73961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA5	TCTTATTCGTTGGAGACTTTATAACCCT	153.07678
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73962_Cmae_Bot2011_COI	MA33	1	BB73962_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Absent	Sample	Cmae_Bot2011_COI	MA33	COI	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	ATGAACAGTCTATCCTCCTTTAG	144.91212
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73962_Hsan_Wil2021_COI	MA33	1	BB73962_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Absent	Sample	Hsan_Wil2021_COI	MA33	COI	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	CCTGGGCCGGTATAGTAGGT	144.91212
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73962_Msal_Bot2012_COI	MA33	1	BB73962_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Absent	Sample	Msal_Bot2012_COI	MA33	COI	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	GYTAGTTCCRATGATGTTAGCTG	144.91212
BB73964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40078	WGS84	BB73964	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73964_Aamu_Bax2006_COI	BA44	1	BB73964_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73964	Sample	BB73964	targeted	Absent	Sample	Aamu_Bax2006_COI	BA44	COI	45	BB73964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA44	GCACAACCGGGATCTTTACTTC	153.16349
BB73964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40078	WGS84	BB73964	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73964_Pcan_Bot2011_IGS	BA44	1	BB73964_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73964	Sample	BB73964	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA44	IGS	45	BB73964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA44	CGTAATCCTCAGTACTGGCTA	153.16349
BB73964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40078	WGS84	BB73964	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73964_Sspa_Oph2007_COI	BA44	1	BB73964_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73964	Sample	BB73964	targeted	Absent	Sample	Sspa_Oph2007_COI	BA44	COI	45	BB73964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA44	GAAGGCCTTCCGATGATC	153.16349
BB73964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40078	WGS84	BB73964	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73964_Upin_Bot2011_COI	BA44	1	BB73964_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73964	Sample	BB73964	targeted	Absent	Sample	Upin_Bot2011_COI	BA44	COI	45	BB73964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA44	CTTTAATTACAGCGTTTTTATTGTTGT	153.16349
BB73965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40104	WGS84	BB73965	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73965_Mgig_Bot2012_COI	BA46	1	BB73965_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73965	Sample	BB73965	targeted	Absent	Sample	Mgig_Bot2012_COI	BA46	COI	45	BB73965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA46	TCTTATTCGTTGGAGACTTTATAACCCT	153.16167
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73966_Cmae_Bot2011_COI	MA27	1	BB73966_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Absent	Sample	Cmae_Bot2011_COI	MA27	COI	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	ATGAACAGTCTATCCTCCTTTAG	144.90642
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73966_Hsan_Wil2021_COI	MA27	1	BB73966_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Absent	Sample	Hsan_Wil2021_COI	MA27	COI	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	CCTGGGCCGGTATAGTAGGT	144.90642
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73966_Msal_Bot2012_COI	MA27	1	BB73966_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Absent	Sample	Msal_Bot2012_COI	MA27	COI	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	GYTAGTTCCRATGATGTTAGCTG	144.90642
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.238	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73968_Aamu_Bax2006_COI	MA24	1	BB73968_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Present	Sample	Aamu_Bax2006_COI	MA24	COI	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	GCACAACCGGGATCTTTACTTC	144.90697
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73968_Pcan_Bot2011_IGS	MA24	1	BB73968_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA24	IGS	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	CGTAATCCTCAGTACTGGCTA	144.90697
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73968_Sspa_Oph2007_COI	MA24	1	BB73968_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Absent	Sample	Sspa_Oph2007_COI	MA24	COI	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	GAAGGCCTTCCGATGATC	144.90697
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73968_Upin_Bot2011_COI	MA24	1	BB73968_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Absent	Sample	Upin_Bot2011_COI	MA24	COI	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	CTTTAATTACAGCGTTTTTATTGTTGT	144.90697
BB73969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40088	WGS84	BB73969	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73969_Mgig_Bot2012_COI	BA48	1	BB73969_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73969	Sample	BB73969	targeted	Absent	Sample	Mgig_Bot2012_COI	BA48	COI	45	BB73969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA48	TCTTATTCGTTGGAGACTTTATAACCCT	153.15618
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.185	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73971_Aamu_Bax2006_COI	MA21	1	BB73971_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Present	Sample	Aamu_Bax2006_COI	MA21	COI	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	GCACAACCGGGATCTTTACTTC	144.91224
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73971_Pcan_Bot2011_IGS	MA21	1	BB73971_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA21	IGS	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	CGTAATCCTCAGTACTGGCTA	144.91224
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73971_Sspa_Oph2007_COI	MA21	1	BB73971_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Absent	Sample	Sspa_Oph2007_COI	MA21	COI	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	GAAGGCCTTCCGATGATC	144.91224
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.705	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73971_Upin_Bot2011_COI	MA21	1	BB73971_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Present	Sample	Upin_Bot2011_COI	MA21	COI	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	CTTTAATTACAGCGTTTTTATTGTTGT	144.91224
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73972_Mgig_Bot2012_COI	MA126	1	BB73972_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Absent	Sample	Mgig_Bot2012_COI	MA126	COI	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	TCTTATTCGTTGGAGACTTTATAACCCT	144.93196
BB73973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40277	WGS84	BB73973	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73973_Cmae_Bot2011_COI	BA49	1	BB73973_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73973	Sample	BB73973	targeted	Absent	Sample	Cmae_Bot2011_COI	BA49	COI	45	BB73973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA49	ATGAACAGTCTATCCTCCTTTAG	153.15406
BB73973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40277	WGS84	BB73973	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73973_Msal_Bot2012_COI	BA49	1	BB73973_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73973	Sample	BB73973	targeted	Absent	Sample	Msal_Bot2012_COI	BA49	COI	45	BB73973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA49	GYTAGTTCCRATGATGTTAGCTG	153.15406
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.485	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73975_Aamu_Bax2006_COI	MA121	1	BB73975_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Present	Sample	Aamu_Bax2006_COI	MA121	COI	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	GCACAACCGGGATCTTTACTTC	144.93272
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73975_Pcan_Bot2011_IGS	MA121	1	BB73975_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA121	IGS	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	CGTAATCCTCAGTACTGGCTA	144.93272
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.552	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73975_Sspa_Oph2007_COI	MA121	1	BB73975_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Present	Sample	Sspa_Oph2007_COI	MA121	COI	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	GAAGGCCTTCCGATGATC	144.93272
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73975_Upin_Bot2011_COI	MA121	1	BB73975_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Absent	Sample	Upin_Bot2011_COI	MA121	COI	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	CTTTAATTACAGCGTTTTTATTGTTGT	144.93272
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73976_Mgig_Bot2012_COI	MA117	1	BB73976_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Absent	Sample	Mgig_Bot2012_COI	MA117	COI	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	TCTTATTCGTTGGAGACTTTATAACCCT	144.92668
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73977_Cmae_Bot2011_COI	MA116	1	BB73977_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Absent	Sample	Cmae_Bot2011_COI	MA116	COI	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	ATGAACAGTCTATCCTCCTTTAG	144.93082
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73977_Hsan_Wil2021_COI	MA116	1	BB73977_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Absent	Sample	Hsan_Wil2021_COI	MA116	COI	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	CCTGGGCCGGTATAGTAGGT	144.93082
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73977_Msal_Bot2012_COI	MA116	1	BB73977_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Absent	Sample	Msal_Bot2012_COI	MA116	COI	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	GYTAGTTCCRATGATGTTAGCTG	144.93082
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.428	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73979_Aamu_Bax2006_COI	MA113	1	BB73979_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Present	Sample	Aamu_Bax2006_COI	MA113	COI	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	GCACAACCGGGATCTTTACTTC	144.92943
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73979_Pcan_Bot2011_IGS	MA113	1	BB73979_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA113	IGS	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	CGTAATCCTCAGTACTGGCTA	144.92943
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.406	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73979_Sspa_Oph2007_COI	MA113	1	BB73979_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Present	Sample	Sspa_Oph2007_COI	MA113	COI	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	GAAGGCCTTCCGATGATC	144.92943
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.282	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73979_Upin_Bot2011_COI	MA113	1	BB73979_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Present	Sample	Upin_Bot2011_COI	MA113	COI	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	CTTTAATTACAGCGTTTTTATTGTTGT	144.92943
BB73980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42093	WGS84	BB73980	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73980_Cmae_Bot2011_COI	BA54	1	BB73980_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73980	Sample	BB73980	targeted	Absent	Sample	Cmae_Bot2011_COI	BA54	COI	45	BB73980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA54	ATGAACAGTCTATCCTCCTTTAG	153.13708
BB73980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42093	WGS84	BB73980	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73980_Msal_Bot2012_COI	BA54	1	BB73980_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73980	Sample	BB73980	targeted	Absent	Sample	Msal_Bot2012_COI	BA54	COI	45	BB73980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA54	GYTAGTTCCRATGATGTTAGCTG	153.13708
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.923	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73982_Aamu_Bax2006_COI	MA66	1	BB73982_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Present	Sample	Aamu_Bax2006_COI	MA66	COI	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	GCACAACCGGGATCTTTACTTC	144.90195
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73982_Pcan_Bot2011_IGS	MA66	1	BB73982_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA66	IGS	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	CGTAATCCTCAGTACTGGCTA	144.90195
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.396	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73982_Sspa_Oph2007_COI	MA66	1	BB73982_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Present	Sample	Sspa_Oph2007_COI	MA66	COI	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	GAAGGCCTTCCGATGATC	144.90195
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73982_Upin_Bot2011_COI	MA66	1	BB73982_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Absent	Sample	Upin_Bot2011_COI	MA66	COI	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	CTTTAATTACAGCGTTTTTATTGTTGT	144.90195
BB73983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80862	WGS84	BB73983	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73983_Mgig_Bot2012_COI	GA12	1	BB73983_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73983	Sample	BB73983	targeted	Absent	Sample	Mgig_Bot2012_COI	GA12	COI	45	BB73983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA12	TCTTATTCGTTGGAGACTTTATAACCCT	151.21623
BB73984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80612	WGS84	BB73984	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73984_Cmae_Bot2011_COI	GA11	1	BB73984_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73984	Sample	BB73984	targeted	Absent	Sample	Cmae_Bot2011_COI	GA11	COI	45	BB73984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA11	ATGAACAGTCTATCCTCCTTTAG	151.21916
BB73984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80612	WGS84	BB73984	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73984_Msal_Bot2012_COI	GA11	1	BB73984_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73984	Sample	BB73984	targeted	Absent	Sample	Msal_Bot2012_COI	GA11	COI	45	BB73984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA11	GYTAGTTCCRATGATGTTAGCTG	151.21916
BB73986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42312	WGS84	BB73986	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73986_Aamu_Bax2006_COI	BA56	1	BB73986_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73986	Sample	BB73986	targeted	Absent	Sample	Aamu_Bax2006_COI	BA56	COI	45	BB73986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA56	GCACAACCGGGATCTTTACTTC	153.13199
BB73986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42312	WGS84	BB73986	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73986_Pcan_Bot2011_IGS	BA56	1	BB73986_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73986	Sample	BB73986	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA56	IGS	45	BB73986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA56	CGTAATCCTCAGTACTGGCTA	153.13199
BB73986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42312	WGS84	BB73986	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73986_Sspa_Oph2007_COI	BA56	1	BB73986_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73986	Sample	BB73986	targeted	Absent	Sample	Sspa_Oph2007_COI	BA56	COI	45	BB73986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA56	GAAGGCCTTCCGATGATC	153.13199
BB73986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42312	WGS84	BB73986	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73986_Upin_Bot2011_COI	BA56	1	BB73986_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73986	Sample	BB73986	targeted	Absent	Sample	Upin_Bot2011_COI	BA56	COI	45	BB73986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA56	CTTTAATTACAGCGTTTTTATTGTTGT	153.13199
BB73987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80807	WGS84	BB73987	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73987_Mgig_Bot2012_COI	GA7	1	BB73987_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73987	Sample	BB73987	targeted	Absent	Sample	Mgig_Bot2012_COI	GA7	COI	45	BB73987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA7	TCTTATTCGTTGGAGACTTTATAACCCT	151.22001
BB73988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42525	WGS84	BB73988	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73988_Cmae_Bot2011_COI	BA57	1	BB73988_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73988	Sample	BB73988	targeted	Absent	Sample	Cmae_Bot2011_COI	BA57	COI	45	BB73988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA57	ATGAACAGTCTATCCTCCTTTAG	153.12768
BB73988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42525	WGS84	BB73988	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73988_Msal_Bot2012_COI	BA57	1	BB73988_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73988	Sample	BB73988	targeted	Absent	Sample	Msal_Bot2012_COI	BA57	COI	45	BB73988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA57	GYTAGTTCCRATGATGTTAGCTG	153.12768
BB73990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42968	WGS84	BB73990	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73990_Mgig_Bot2012_COI	BA61	1	BB73990_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73990	Sample	BB73990	targeted	Absent	Sample	Mgig_Bot2012_COI	BA61	COI	45	BB73990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA61	TCTTATTCGTTGGAGACTTTATAACCCT	153.12444
BB73991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80628	WGS84	BB73991	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73991_Cmae_Bot2011_COI	GA2	1	BB73991_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73991	Sample	BB73991	targeted	Absent	Sample	Cmae_Bot2011_COI	GA2	COI	45	BB73991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA2	ATGAACAGTCTATCCTCCTTTAG	151.21494
BB73991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80628	WGS84	BB73991	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73991_Msal_Bot2012_COI	GA2	1	BB73991_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73991	Sample	BB73991	targeted	Absent	Sample	Msal_Bot2012_COI	GA2	COI	45	BB73991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA2	GYTAGTTCCRATGATGTTAGCTG	151.21494
BB73993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85375	WGS84	BB73993	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73993_Aamu_Bax2006_COI	MA35	1	BB73993_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73993	Sample	BB73993	targeted	Absent	Sample	Aamu_Bax2006_COI	MA35	COI	45	BB73993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA35	GCACAACCGGGATCTTTACTTC	151.30827
BB73993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85375	WGS84	BB73993	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73993_Pcan_Bot2011_IGS	MA35	1	BB73993_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73993	Sample	BB73993	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA35	IGS	45	BB73993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA35	CGTAATCCTCAGTACTGGCTA	151.30827
BB73993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85375	WGS84	BB73993	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73993_Sspa_Oph2007_COI	MA35	1	BB73993_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73993	Sample	BB73993	targeted	Absent	Sample	Sspa_Oph2007_COI	MA35	COI	45	BB73993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA35	GAAGGCCTTCCGATGATC	151.30827
BB73993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85375	WGS84	BB73993	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73993_Upin_Bot2011_COI	MA35	1	BB73993_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73993	Sample	BB73993	targeted	Absent	Sample	Upin_Bot2011_COI	MA35	COI	45	BB73993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA35	CTTTAATTACAGCGTTTTTATTGTTGT	151.30827
BB73994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85266	WGS84	BB73994	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73994_Mgig_Bot2012_COI	MA30	1	BB73994_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73994	Sample	BB73994	targeted	Absent	Sample	Mgig_Bot2012_COI	MA30	COI	45	BB73994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA30	TCTTATTCGTTGGAGACTTTATAACCCT	151.31613
BB73995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85533	WGS84	BB73995	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73995_Cmae_Bot2011_COI	MA25	1	BB73995_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73995	Sample	BB73995	targeted	Absent	Sample	Cmae_Bot2011_COI	MA25	COI	45	BB73995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA25	ATGAACAGTCTATCCTCCTTTAG	151.32178
BB73995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85533	WGS84	BB73995	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73995_Msal_Bot2012_COI	MA25	1	BB73995_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73995	Sample	BB73995	targeted	Absent	Sample	Msal_Bot2012_COI	MA25	COI	45	BB73995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA25	GYTAGTTCCRATGATGTTAGCTG	151.32178
BB73997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85066	WGS84	BB73997	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73997_Aamu_Bax2006_COI	GA33	1	BB73997_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73997	Sample	BB73997	targeted	Absent	Sample	Aamu_Bax2006_COI	GA33	COI	45	BB73997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA33	GCACAACCGGGATCTTTACTTC	151.31174
BB73997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85066	WGS84	BB73997	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73997_Pcan_Bot2011_IGS	GA33	1	BB73997_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73997	Sample	BB73997	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA33	IGS	45	BB73997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA33	CGTAATCCTCAGTACTGGCTA	151.31174
BB73997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85066	WGS84	BB73997	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73997_Sspa_Oph2007_COI	GA33	1	BB73997_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73997	Sample	BB73997	targeted	Absent	Sample	Sspa_Oph2007_COI	GA33	COI	45	BB73997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA33	GAAGGCCTTCCGATGATC	151.31174
BB73997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85066	WGS84	BB73997	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73997_Upin_Bot2011_COI	GA33	1	BB73997_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73997	Sample	BB73997	targeted	Absent	Sample	Upin_Bot2011_COI	GA33	COI	45	BB73997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA33	CTTTAATTACAGCGTTTTTATTGTTGT	151.31174
BB73998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38217	WGS84	BB73998	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73998_Mgig_Bot2012_COI	BA37	1	BB73998_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73998	Sample	BB73998	targeted	Absent	Sample	Mgig_Bot2012_COI	BA37	COI	45	BB73998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA37	TCTTATTCGTTGGAGACTTTATAACCCT	153.16148
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73999_Cmae_Bot2011_COI	MA39	1	BB73999_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Absent	Sample	Cmae_Bot2011_COI	MA39	COI	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	ATGAACAGTCTATCCTCCTTTAG	144.90391
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73999_Hsan_Wil2021_COI	MA39	1	BB73999_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Absent	Sample	Hsan_Wil2021_COI	MA39	COI	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	CCTGGGCCGGTATAGTAGGT	144.90391
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73999_Msal_Bot2012_COI	MA39	1	BB73999_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Absent	Sample	Msal_Bot2012_COI	MA39	COI	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	GYTAGTTCCRATGATGTTAGCTG	144.90391
BB74000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85211	WGS84	BB74000	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74000_Aamu_Bax2006_COI	GA29	1	BB74000_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74000	Sample	BB74000	targeted	Absent	Sample	Aamu_Bax2006_COI	GA29	COI	45	BB74000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA29	GCACAACCGGGATCTTTACTTC	151.31866
BB74000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85211	WGS84	BB74000	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74000_Pcan_Bot2011_IGS	GA29	1	BB74000_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74000	Sample	BB74000	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA29	IGS	45	BB74000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA29	CGTAATCCTCAGTACTGGCTA	151.31866
BB74000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85211	WGS84	BB74000	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74000_Sspa_Oph2007_COI	GA29	1	BB74000_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74000	Sample	BB74000	targeted	Absent	Sample	Sspa_Oph2007_COI	GA29	COI	45	BB74000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA29	GAAGGCCTTCCGATGATC	151.31866
BB74000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85211	WGS84	BB74000	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74000_Upin_Bot2011_COI	GA29	1	BB74000_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74000	Sample	BB74000	targeted	Absent	Sample	Upin_Bot2011_COI	GA29	COI	45	BB74000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA29	CTTTAATTACAGCGTTTTTATTGTTGT	151.31866
BB74001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88481	WGS84	BB74001	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74001_Mgig_Bot2012_COI	HA2	1	BB74001_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74001	Sample	BB74001	targeted	Absent	Sample	Mgig_Bot2012_COI	HA2	COI	45	BB74001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA2	TCTTATTCGTTGGAGACTTTATAACCCT	147.3442
BB74002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85391	WGS84	BB74002	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74002_Cmae_Bot2011_COI	GA27	1	BB74002_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74002	Sample	BB74002	targeted	Absent	Sample	Cmae_Bot2011_COI	GA27	COI	45	BB74002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA27	ATGAACAGTCTATCCTCCTTTAG	151.31835
BB74002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85391	WGS84	BB74002	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74002_Msal_Bot2012_COI	GA27	1	BB74002_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74002	Sample	BB74002	targeted	Absent	Sample	Msal_Bot2012_COI	GA27	COI	45	BB74002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA27	GYTAGTTCCRATGATGTTAGCTG	151.31835
BB74004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85331	WGS84	BB74004	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74004_Aamu_Bax2006_COI	GA24	1	BB74004_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74004	Sample	BB74004	targeted	Absent	Sample	Aamu_Bax2006_COI	GA24	COI	45	BB74004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA24	GCACAACCGGGATCTTTACTTC	151.32146
BB74004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85331	WGS84	BB74004	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74004_Pcan_Bot2011_IGS	GA24	1	BB74004_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74004	Sample	BB74004	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA24	IGS	45	BB74004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA24	CGTAATCCTCAGTACTGGCTA	151.32146
BB74004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85331	WGS84	BB74004	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74004_Sspa_Oph2007_COI	GA24	1	BB74004_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74004	Sample	BB74004	targeted	Absent	Sample	Sspa_Oph2007_COI	GA24	COI	45	BB74004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA24	GAAGGCCTTCCGATGATC	151.32146
BB74004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85331	WGS84	BB74004	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74004_Upin_Bot2011_COI	GA24	1	BB74004_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74004	Sample	BB74004	targeted	Absent	Sample	Upin_Bot2011_COI	GA24	COI	45	BB74004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA24	CTTTAATTACAGCGTTTTTATTGTTGT	151.32146
BB74005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86492	WGS84	BB74005	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	40.497	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74005_Mgig_Bot2012_COI	MA77	1	BB74005_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74005	Sample	BB74005	targeted	Present	Sample	Mgig_Bot2012_COI	MA77	COI	45	BB74005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA77	TCTTATTCGTTGGAGACTTTATAACCCT	144.91599
BB74006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8879	WGS84	BB74006	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74006_Cmae_Bot2011_COI	HA4	1	BB74006_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74006	Sample	BB74006	targeted	Absent	Sample	Cmae_Bot2011_COI	HA4	COI	45	BB74006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA4	ATGAACAGTCTATCCTCCTTTAG	147.3418
BB74006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8879	WGS84	BB74006	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74006_Msal_Bot2012_COI	HA4	1	BB74006_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74006	Sample	BB74006	targeted	Absent	Sample	Msal_Bot2012_COI	HA4	COI	45	BB74006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA4	GYTAGTTCCRATGATGTTAGCTG	147.3418
BB74008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78775	WGS84	BB74008	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74008_Aamu_Bax2006_COI	MA55	1	BB74008_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74008	Sample	BB74008	targeted	Absent	Sample	Aamu_Bax2006_COI	MA55	COI	45	BB74008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA55	GCACAACCGGGATCTTTACTTC	151.17457
BB74008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78775	WGS84	BB74008	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74008_Pcan_Bot2011_IGS	MA55	1	BB74008_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74008	Sample	BB74008	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA55	IGS	45	BB74008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA55	CGTAATCCTCAGTACTGGCTA	151.17457
BB74008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78775	WGS84	BB74008	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74008_Sspa_Oph2007_COI	MA55	1	BB74008_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74008	Sample	BB74008	targeted	Absent	Sample	Sspa_Oph2007_COI	MA55	COI	45	BB74008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA55	GAAGGCCTTCCGATGATC	151.17457
BB74008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78775	WGS84	BB74008	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74008_Upin_Bot2011_COI	MA55	1	BB74008_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74008	Sample	BB74008	targeted	Absent	Sample	Upin_Bot2011_COI	MA55	COI	45	BB74008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA55	CTTTAATTACAGCGTTTTTATTGTTGT	151.17457
BB74009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81714	WGS84	BB74009	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74009_Mgig_Bot2012_COI	MA88	1	BB74009_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74009	Sample	BB74009	targeted	Absent	Sample	Mgig_Bot2012_COI	MA88	COI	45	BB74009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA88	TCTTATTCGTTGGAGACTTTATAACCCT	144.92188
BB74011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78735	WGS84	BB74011	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74011_Aamu_Bax2006_COI	GA62	1	BB74011_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74011	Sample	BB74011	targeted	Absent	Sample	Aamu_Bax2006_COI	GA62	COI	45	BB74011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA62	GCACAACCGGGATCTTTACTTC	151.17788
BB74011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78735	WGS84	BB74011	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74011_Pcan_Bot2011_IGS	GA62	1	BB74011_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74011	Sample	BB74011	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA62	IGS	45	BB74011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA62	CGTAATCCTCAGTACTGGCTA	151.17788
BB74011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78735	WGS84	BB74011	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74011_Sspa_Oph2007_COI	GA62	1	BB74011_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74011	Sample	BB74011	targeted	Absent	Sample	Sspa_Oph2007_COI	GA62	COI	45	BB74011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA62	GAAGGCCTTCCGATGATC	151.17788
BB74011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78735	WGS84	BB74011	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74011_Upin_Bot2011_COI	GA62	1	BB74011_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74011	Sample	BB74011	targeted	Absent	Sample	Upin_Bot2011_COI	GA62	COI	45	BB74011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA62	CTTTAATTACAGCGTTTTTATTGTTGT	151.17788
BB74012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87588	WGS84	BB74012	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74012_Mgig_Bot2012_COI	HA6	1	BB74012_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74012	Sample	BB74012	targeted	Absent	Sample	Mgig_Bot2012_COI	HA6	COI	45	BB74012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA6	TCTTATTCGTTGGAGACTTTATAACCCT	147.34299
BB74013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87954	WGS84	BB74013	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	42.355	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74013_Cmae_Bot2011_COI	HA7	1	BB74013_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74013	Sample	BB74013	targeted	Present	Sample	Cmae_Bot2011_COI	HA7	COI	45	BB74013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA7	ATGAACAGTCTATCCTCCTTTAG	147.34488
BB74013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87954	WGS84	BB74013	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74013_Msal_Bot2012_COI	HA7	1	BB74013_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74013	Sample	BB74013	targeted	Absent	Sample	Msal_Bot2012_COI	HA7	COI	45	BB74013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA7	GYTAGTTCCRATGATGTTAGCTG	147.34488
BB74015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78668	WGS84	BB74015	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74015_Aamu_Bax2006_COI	GA56	1	BB74015_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74015	Sample	BB74015	targeted	Absent	Sample	Aamu_Bax2006_COI	GA56	COI	45	BB74015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA56	GCACAACCGGGATCTTTACTTC	151.17519
BB74015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78668	WGS84	BB74015	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74015_Pcan_Bot2011_IGS	GA56	1	BB74015_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74015	Sample	BB74015	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA56	IGS	45	BB74015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA56	CGTAATCCTCAGTACTGGCTA	151.17519
BB74015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78668	WGS84	BB74015	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74015_Sspa_Oph2007_COI	GA56	1	BB74015_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74015	Sample	BB74015	targeted	Absent	Sample	Sspa_Oph2007_COI	GA56	COI	45	BB74015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA56	GAAGGCCTTCCGATGATC	151.17519
BB74015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78668	WGS84	BB74015	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74015_Upin_Bot2011_COI	GA56	1	BB74015_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74015	Sample	BB74015	targeted	Absent	Sample	Upin_Bot2011_COI	GA56	COI	45	BB74015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA56	CTTTAATTACAGCGTTTTTATTGTTGT	151.17519
BB74016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88575	WGS84	BB74016	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	42.817	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74016_Mgig_Bot2012_COI	HA8	1	BB74016_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74016	Sample	BB74016	targeted	Present	Sample	Mgig_Bot2012_COI	HA8	COI	45	BB74016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA8	TCTTATTCGTTGGAGACTTTATAACCCT	147.33759
BB74017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78949	WGS84	BB74017	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74017_Cmae_Bot2011_COI	GA53	1	BB74017_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74017	Sample	BB74017	targeted	Absent	Sample	Cmae_Bot2011_COI	GA53	COI	45	BB74017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA53	ATGAACAGTCTATCCTCCTTTAG	151.17594
BB74017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78949	WGS84	BB74017	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74017_Msal_Bot2012_COI	GA53	1	BB74017_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74017	Sample	BB74017	targeted	Absent	Sample	Msal_Bot2012_COI	GA53	COI	45	BB74017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA53	GYTAGTTCCRATGATGTTAGCTG	151.17594
BB74019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78956	WGS84	BB74019	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74019_Aamu_Bax2006_COI	GA51	1	BB74019_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74019	Sample	BB74019	targeted	Absent	Sample	Aamu_Bax2006_COI	GA51	COI	45	BB74019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA51	GCACAACCGGGATCTTTACTTC	151.17817
BB74019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78956	WGS84	BB74019	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74019_Pcan_Bot2011_IGS	GA51	1	BB74019_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74019	Sample	BB74019	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA51	IGS	45	BB74019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA51	CGTAATCCTCAGTACTGGCTA	151.17817
BB74019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78956	WGS84	BB74019	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74019_Sspa_Oph2007_COI	GA51	1	BB74019_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74019	Sample	BB74019	targeted	Absent	Sample	Sspa_Oph2007_COI	GA51	COI	45	BB74019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA51	GAAGGCCTTCCGATGATC	151.17817
BB74019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78956	WGS84	BB74019	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74019_Upin_Bot2011_COI	GA51	1	BB74019_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74019	Sample	BB74019	targeted	Absent	Sample	Upin_Bot2011_COI	GA51	COI	45	BB74019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA51	CTTTAATTACAGCGTTTTTATTGTTGT	151.17817
BB74020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79134	WGS84	BB74020	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74020_Cmae_Bot2011_COI	GA49	1	BB74020_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74020	Sample	BB74020	targeted	Absent	Sample	Cmae_Bot2011_COI	GA49	COI	45	BB74020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA49	ATGAACAGTCTATCCTCCTTTAG	151.18417
BB74020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79134	WGS84	BB74020	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74020_Msal_Bot2012_COI	GA49	1	BB74020_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74020	Sample	BB74020	targeted	Absent	Sample	Msal_Bot2012_COI	GA49	COI	45	BB74020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA49	GYTAGTTCCRATGATGTTAGCTG	151.18417
BB74022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79159	WGS84	BB74022	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74022_Aamu_Bax2006_COI	GA47	1	BB74022_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74022	Sample	BB74022	targeted	Absent	Sample	Aamu_Bax2006_COI	GA47	COI	45	BB74022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA47	GCACAACCGGGATCTTTACTTC	151.1805
BB74022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79159	WGS84	BB74022	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74022_Pcan_Bot2011_IGS	GA47	1	BB74022_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74022	Sample	BB74022	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA47	IGS	45	BB74022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA47	CGTAATCCTCAGTACTGGCTA	151.1805
BB74022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79159	WGS84	BB74022	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74022_Sspa_Oph2007_COI	GA47	1	BB74022_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74022	Sample	BB74022	targeted	Absent	Sample	Sspa_Oph2007_COI	GA47	COI	45	BB74022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA47	GAAGGCCTTCCGATGATC	151.1805
BB74022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79159	WGS84	BB74022	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74022_Upin_Bot2011_COI	GA47	1	BB74022_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74022	Sample	BB74022	targeted	Absent	Sample	Upin_Bot2011_COI	GA47	COI	45	BB74022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA47	CTTTAATTACAGCGTTTTTATTGTTGT	151.1805
BB74023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88275	WGS84	BB74023	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74023_Mgig_Bot2012_COI	HA12	1	BB74023_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74023	Sample	BB74023	targeted	Absent	Sample	Mgig_Bot2012_COI	HA12	COI	45	BB74023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA12	TCTTATTCGTTGGAGACTTTATAACCCT	147.33625
BB74024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78496	WGS84	BB74024	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74024_Cmae_Bot2011_COI	MA70	1	BB74024_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74024	Sample	BB74024	targeted	Absent	Sample	Cmae_Bot2011_COI	MA70	COI	45	BB74024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA70	ATGAACAGTCTATCCTCCTTTAG	151.20333
BB74024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78496	WGS84	BB74024	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74024_Msal_Bot2012_COI	MA70	1	BB74024_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74024	Sample	BB74024	targeted	Absent	Sample	Msal_Bot2012_COI	MA70	COI	45	BB74024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA70	GYTAGTTCCRATGATGTTAGCTG	151.20333
BB74026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84997	WGS84	BB74026	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.962	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74026_Aamu_Bax2006_COI	HA14	1	BB74026_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74026	Sample	BB74026	targeted	Present	Sample	Aamu_Bax2006_COI	HA14	COI	45	BB74026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA14	GCACAACCGGGATCTTTACTTC	147.33006
BB74026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84997	WGS84	BB74026	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74026_Pcan_Bot2011_IGS	HA14	1	BB74026_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74026	Sample	BB74026	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA14	IGS	45	BB74026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA14	CGTAATCCTCAGTACTGGCTA	147.33006
BB74026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84997	WGS84	BB74026	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74026_Sspa_Oph2007_COI	HA14	1	BB74026_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74026	Sample	BB74026	targeted	Absent	Sample	Sspa_Oph2007_COI	HA14	COI	45	BB74026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA14	GAAGGCCTTCCGATGATC	147.33006
BB74026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84997	WGS84	BB74026	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74026_Upin_Bot2011_COI	HA14	1	BB74026_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74026	Sample	BB74026	targeted	Absent	Sample	Upin_Bot2011_COI	HA14	COI	45	BB74026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA14	CTTTAATTACAGCGTTTTTATTGTTGT	147.33006
BB74027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78359	WGS84	BB74027	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74027_Mgig_Bot2012_COI	GA68	1	BB74027_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74027	Sample	BB74027	targeted	Absent	Sample	Mgig_Bot2012_COI	GA68	COI	45	BB74027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA68	TCTTATTCGTTGGAGACTTTATAACCCT	151.20135
BB74028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7813	WGS84	BB74028	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74028_Cmae_Bot2011_COI	GA67	1	BB74028_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74028	Sample	BB74028	targeted	Absent	Sample	Cmae_Bot2011_COI	GA67	COI	45	BB74028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA67	ATGAACAGTCTATCCTCCTTTAG	151.1994
BB74028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7813	WGS84	BB74028	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74028_Msal_Bot2012_COI	GA67	1	BB74028_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74028	Sample	BB74028	targeted	Absent	Sample	Msal_Bot2012_COI	GA67	COI	45	BB74028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA67	GYTAGTTCCRATGATGTTAGCTG	151.1994
BB74030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84551	WGS84	BB74030	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74030_Mgig_Bot2012_COI	HA17	1	BB74030_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74030	Sample	BB74030	targeted	Absent	Sample	Mgig_Bot2012_COI	HA17	COI	45	BB74030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA17	TCTTATTCGTTGGAGACTTTATAACCCT	147.33086
BB74031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77756	WGS84	BB74031	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74031_Cmae_Bot2011_COI	GA63	1	BB74031_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74031	Sample	BB74031	targeted	Absent	Sample	Cmae_Bot2011_COI	GA63	COI	45	BB74031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA63	ATGAACAGTCTATCCTCCTTTAG	151.19502
BB74031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77756	WGS84	BB74031	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74031_Msal_Bot2012_COI	GA63	1	BB74031_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74031	Sample	BB74031	targeted	Absent	Sample	Msal_Bot2012_COI	GA63	COI	45	BB74031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA63	GYTAGTTCCRATGATGTTAGCTG	151.19502
BB74033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79513	WGS84	BB74033	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74033_Aamu_Bax2006_COI	GA111	1	BB74033_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74033	Sample	BB74033	targeted	Absent	Sample	Aamu_Bax2006_COI	GA111	COI	45	BB74033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA111	GCACAACCGGGATCTTTACTTC	151.21416
BB74033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79513	WGS84	BB74033	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74033_Pcan_Bot2011_IGS	GA111	1	BB74033_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74033	Sample	BB74033	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA111	IGS	45	BB74033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA111	CGTAATCCTCAGTACTGGCTA	151.21416
BB74033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79513	WGS84	BB74033	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74033_Sspa_Oph2007_COI	GA111	1	BB74033_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74033	Sample	BB74033	targeted	Absent	Sample	Sspa_Oph2007_COI	GA111	COI	45	BB74033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA111	GAAGGCCTTCCGATGATC	151.21416
BB74033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79513	WGS84	BB74033	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74033_Upin_Bot2011_COI	GA111	1	BB74033_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74033	Sample	BB74033	targeted	Absent	Sample	Upin_Bot2011_COI	GA111	COI	45	BB74033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA111	CTTTAATTACAGCGTTTTTATTGTTGT	151.21416
BB74034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8293	WGS84	BB74034	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74034_Mgig_Bot2012_COI	HA22	1	BB74034_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74034	Sample	BB74034	targeted	Absent	Sample	Mgig_Bot2012_COI	HA22	COI	45	BB74034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA22	TCTTATTCGTTGGAGACTTTATAACCCT	147.3183
BB74035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79115	WGS84	BB74035	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74035_Cmae_Bot2011_COI	GA72	1	BB74035_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74035	Sample	BB74035	targeted	Absent	Sample	Cmae_Bot2011_COI	GA72	COI	45	BB74035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA72	ATGAACAGTCTATCCTCCTTTAG	151.2105
BB74035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79115	WGS84	BB74035	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74035_Msal_Bot2012_COI	GA72	1	BB74035_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74035	Sample	BB74035	targeted	Absent	Sample	Msal_Bot2012_COI	GA72	COI	45	BB74035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA72	GYTAGTTCCRATGATGTTAGCTG	151.2105
BB74037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82416	WGS84	BB74037	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74037_Aamu_Bax2006_COI	GA103	1	BB74037_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74037	Sample	BB74037	targeted	Absent	Sample	Aamu_Bax2006_COI	GA103	COI	45	BB74037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA103	GCACAACCGGGATCTTTACTTC	151.24835
BB74037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82416	WGS84	BB74037	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74037_Pcan_Bot2011_IGS	GA103	1	BB74037_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74037	Sample	BB74037	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA103	IGS	45	BB74037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA103	CGTAATCCTCAGTACTGGCTA	151.24835
BB74037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82416	WGS84	BB74037	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74037_Sspa_Oph2007_COI	GA103	1	BB74037_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74037	Sample	BB74037	targeted	Absent	Sample	Sspa_Oph2007_COI	GA103	COI	45	BB74037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA103	GAAGGCCTTCCGATGATC	151.24835
BB74037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82416	WGS84	BB74037	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74037_Upin_Bot2011_COI	GA103	1	BB74037_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74037	Sample	BB74037	targeted	Absent	Sample	Upin_Bot2011_COI	GA103	COI	45	BB74037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA103	CTTTAATTACAGCGTTTTTATTGTTGT	151.24835
BB74038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81951	WGS84	BB74038	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74038_Mgig_Bot2012_COI	MA105	1	BB74038_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74038	Sample	BB74038	targeted	Absent	Sample	Mgig_Bot2012_COI	MA105	COI	45	BB74038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA105	TCTTATTCGTTGGAGACTTTATAACCCT	151.24366
BB74039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.83217	WGS84	BB74039	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74039_Cmae_Bot2011_COI	HA24	1	BB74039_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74039	Sample	BB74039	targeted	Absent	Sample	Cmae_Bot2011_COI	HA24	COI	45	BB74039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA24	ATGAACAGTCTATCCTCCTTTAG	147.32211
BB74039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.83217	WGS84	BB74039	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74039_Msal_Bot2012_COI	HA24	1	BB74039_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74039	Sample	BB74039	targeted	Absent	Sample	Msal_Bot2012_COI	HA24	COI	45	BB74039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA24	GYTAGTTCCRATGATGTTAGCTG	147.32211
BB74040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82597	WGS84	BB74040	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74040_Aamu_Bax2006_COI	MA95	1	BB74040_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74040	Sample	BB74040	targeted	Absent	Sample	Aamu_Bax2006_COI	MA95	COI	45	BB74040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA95	GCACAACCGGGATCTTTACTTC	151.24899
BB74040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82597	WGS84	BB74040	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74040_Pcan_Bot2011_IGS	MA95	1	BB74040_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74040	Sample	BB74040	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA95	IGS	45	BB74040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA95	CGTAATCCTCAGTACTGGCTA	151.24899
BB74040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82597	WGS84	BB74040	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74040_Sspa_Oph2007_COI	MA95	1	BB74040_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74040	Sample	BB74040	targeted	Absent	Sample	Sspa_Oph2007_COI	MA95	COI	45	BB74040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA95	GAAGGCCTTCCGATGATC	151.24899
BB74040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82597	WGS84	BB74040	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74040_Upin_Bot2011_COI	MA95	1	BB74040_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74040	Sample	BB74040	targeted	Absent	Sample	Upin_Bot2011_COI	MA95	COI	45	BB74040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA95	CTTTAATTACAGCGTTTTTATTGTTGT	151.24899
BB74041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82257	WGS84	BB74041	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74041_Mgig_Bot2012_COI	MA90	1	BB74041_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74041	Sample	BB74041	targeted	Absent	Sample	Mgig_Bot2012_COI	MA90	COI	45	BB74041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA90	TCTTATTCGTTGGAGACTTTATAACCCT	151.24362
BB74042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82275	WGS84	BB74042	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74042_Cmae_Bot2011_COI	MA71	1	BB74042_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74042	Sample	BB74042	targeted	Absent	Sample	Cmae_Bot2011_COI	MA71	COI	45	BB74042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA71	ATGAACAGTCTATCCTCCTTTAG	144.90789
BB74042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82275	WGS84	BB74042	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74042_Msal_Bot2012_COI	MA71	1	BB74042_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74042	Sample	BB74042	targeted	Absent	Sample	Msal_Bot2012_COI	MA71	COI	45	BB74042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA71	GYTAGTTCCRATGATGTTAGCTG	144.90789
BB74044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88449	WGS84	BB74044	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.643	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74044_Aamu_Bax2006_COI	HA10	1	BB74044_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74044	Sample	BB74044	targeted	Present	Sample	Aamu_Bax2006_COI	HA10	COI	45	BB74044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA10	GCACAACCGGGATCTTTACTTC	147.33547
BB74044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88449	WGS84	BB74044	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74044_Pcan_Bot2011_IGS	HA10	1	BB74044_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74044	Sample	BB74044	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA10	IGS	45	BB74044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA10	CGTAATCCTCAGTACTGGCTA	147.33547
BB74044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88449	WGS84	BB74044	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74044_Sspa_Oph2007_COI	HA10	1	BB74044_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74044	Sample	BB74044	targeted	Absent	Sample	Sspa_Oph2007_COI	HA10	COI	45	BB74044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA10	GAAGGCCTTCCGATGATC	147.33547
BB74044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88449	WGS84	BB74044	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74044_Upin_Bot2011_COI	HA10	1	BB74044_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74044	Sample	BB74044	targeted	Absent	Sample	Upin_Bot2011_COI	HA10	COI	45	BB74044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA10	CTTTAATTACAGCGTTTTTATTGTTGT	147.33547
BB74045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81955	WGS84	BB74045	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.806	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74045_Mgig_Bot2012_COI	MA91	1	BB74045_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74045	Sample	BB74045	targeted	Present	Sample	Mgig_Bot2012_COI	MA91	COI	45	BB74045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA91	TCTTATTCGTTGGAGACTTTATAACCCT	144.92944
BB74046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81672	WGS84	BB74046	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74046_Cmae_Bot2011_COI	GA107	1	BB74046_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74046	Sample	BB74046	targeted	Absent	Sample	Cmae_Bot2011_COI	GA107	COI	45	BB74046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA107	ATGAACAGTCTATCCTCCTTTAG	151.24042
BB74046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81672	WGS84	BB74046	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74046_Msal_Bot2012_COI	GA107	1	BB74046_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74046	Sample	BB74046	targeted	Absent	Sample	Msal_Bot2012_COI	GA107	COI	45	BB74046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA107	GYTAGTTCCRATGATGTTAGCTG	151.24042
BB74048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82161	WGS84	BB74048	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74048_Aamu_Bax2006_COI	GA104	1	BB74048_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74048	Sample	BB74048	targeted	Absent	Sample	Aamu_Bax2006_COI	GA104	COI	45	BB74048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA104	GCACAACCGGGATCTTTACTTC	151.24523
BB74048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82161	WGS84	BB74048	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74048_Pcan_Bot2011_IGS	GA104	1	BB74048_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74048	Sample	BB74048	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA104	IGS	45	BB74048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA104	CGTAATCCTCAGTACTGGCTA	151.24523
BB74048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82161	WGS84	BB74048	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74048_Sspa_Oph2007_COI	GA104	1	BB74048_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74048	Sample	BB74048	targeted	Absent	Sample	Sspa_Oph2007_COI	GA104	COI	45	BB74048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA104	GAAGGCCTTCCGATGATC	151.24523
BB74048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82161	WGS84	BB74048	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74048_Upin_Bot2011_COI	GA104	1	BB74048_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74048	Sample	BB74048	targeted	Absent	Sample	Upin_Bot2011_COI	GA104	COI	45	BB74048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA104	CTTTAATTACAGCGTTTTTATTGTTGT	151.24523
BB74049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82484	WGS84	BB74049	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74049_Mgig_Bot2012_COI	MA62	1	BB74049_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74049	Sample	BB74049	targeted	Absent	Sample	Mgig_Bot2012_COI	MA62	COI	45	BB74049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA62	TCTTATTCGTTGGAGACTTTATAACCCT	144.90426
BB74051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81866	WGS84	BB74051	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74051_Aamu_Bax2006_COI	GA99	1	BB74051_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74051	Sample	BB74051	targeted	Absent	Sample	Aamu_Bax2006_COI	GA99	COI	45	BB74051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA99	GCACAACCGGGATCTTTACTTC	151.23785
BB74051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81866	WGS84	BB74051	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74051_Pcan_Bot2011_IGS	GA99	1	BB74051_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74051	Sample	BB74051	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA99	IGS	45	BB74051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA99	CGTAATCCTCAGTACTGGCTA	151.23785
BB74051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81866	WGS84	BB74051	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74051_Sspa_Oph2007_COI	GA99	1	BB74051_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74051	Sample	BB74051	targeted	Absent	Sample	Sspa_Oph2007_COI	GA99	COI	45	BB74051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA99	GAAGGCCTTCCGATGATC	151.23785
BB74051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81866	WGS84	BB74051	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74051_Upin_Bot2011_COI	GA99	1	BB74051_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74051	Sample	BB74051	targeted	Absent	Sample	Upin_Bot2011_COI	GA99	COI	45	BB74051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA99	CTTTAATTACAGCGTTTTTATTGTTGT	151.23785
BB74052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84447	WGS84	BB74052	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74052_Mgig_Bot2012_COI	HA20	1	BB74052_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74052	Sample	BB74052	targeted	Absent	Sample	Mgig_Bot2012_COI	HA20	COI	45	BB74052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA20	TCTTATTCGTTGGAGACTTTATAACCCT	147.32885
BB74053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82761	WGS84	BB74053	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74053_Cmae_Bot2011_COI	GA97	1	BB74053_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74053	Sample	BB74053	targeted	Absent	Sample	Cmae_Bot2011_COI	GA97	COI	45	BB74053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA97	ATGAACAGTCTATCCTCCTTTAG	151.24723
BB74053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82761	WGS84	BB74053	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74053_Msal_Bot2012_COI	GA97	1	BB74053_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74053	Sample	BB74053	targeted	Absent	Sample	Msal_Bot2012_COI	GA97	COI	45	BB74053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA97	GYTAGTTCCRATGATGTTAGCTG	151.24723
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.805	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74055_Aamu_Bax2006_COI	MA118	1	BB74055_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Present	Sample	Aamu_Bax2006_COI	MA118	COI	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	GCACAACCGGGATCTTTACTTC	144.92535
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74055_Pcan_Bot2011_IGS	MA118	1	BB74055_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA118	IGS	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	CGTAATCCTCAGTACTGGCTA	144.92535
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.843	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74055_Sspa_Oph2007_COI	MA118	1	BB74055_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Present	Sample	Sspa_Oph2007_COI	MA118	COI	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	GAAGGCCTTCCGATGATC	144.92535
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74055_Upin_Bot2011_COI	MA118	1	BB74055_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Absent	Sample	Upin_Bot2011_COI	MA118	COI	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	CTTTAATTACAGCGTTTTTATTGTTGT	144.92535
BB74056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82353	WGS84	BB74056	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74056_Mgig_Bot2012_COI	GA93	1	BB74056_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74056	Sample	BB74056	targeted	Absent	Sample	Mgig_Bot2012_COI	GA93	COI	45	BB74056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA93	TCTTATTCGTTGGAGACTTTATAACCCT	151.24481
BB74057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82383	WGS84	BB74057	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74057_Cmae_Bot2011_COI	GA92	1	BB74057_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74057	Sample	BB74057	targeted	Absent	Sample	Cmae_Bot2011_COI	GA92	COI	45	BB74057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA92	ATGAACAGTCTATCCTCCTTTAG	151.24413
BB74057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82383	WGS84	BB74057	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74057_Msal_Bot2012_COI	GA92	1	BB74057_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74057	Sample	BB74057	targeted	Absent	Sample	Msal_Bot2012_COI	GA92	COI	45	BB74057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA92	GYTAGTTCCRATGATGTTAGCTG	151.24413
BB74059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44339	WGS84	BB74059	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74059_Aamu_Bax2006_COI	BA1	1	BB74059_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74059	Sample	BB74059	targeted	Absent	Sample	Aamu_Bax2006_COI	BA1	COI	45	BB74059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA1	GCACAACCGGGATCTTTACTTC	153.07218
BB74059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44339	WGS84	BB74059	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74059_Pcan_Bot2011_IGS	BA1	1	BB74059_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74059	Sample	BB74059	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA1	IGS	45	BB74059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA1	CGTAATCCTCAGTACTGGCTA	153.07218
BB74059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44339	WGS84	BB74059	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74059_Sspa_Oph2007_COI	BA1	1	BB74059_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74059	Sample	BB74059	targeted	Absent	Sample	Sspa_Oph2007_COI	BA1	COI	45	BB74059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA1	GAAGGCCTTCCGATGATC	153.07218
BB74059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44339	WGS84	BB74059	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74059_Upin_Bot2011_COI	BA1	1	BB74059_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74059	Sample	BB74059	targeted	Absent	Sample	Upin_Bot2011_COI	BA1	COI	45	BB74059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA1	CTTTAATTACAGCGTTTTTATTGTTGT	153.07218
BB74060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82381	WGS84	BB74060	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74060_Cmae_Bot2011_COI	GA88	1	BB74060_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74060	Sample	BB74060	targeted	Absent	Sample	Cmae_Bot2011_COI	GA88	COI	45	BB74060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA88	ATGAACAGTCTATCCTCCTTTAG	151.24304
BB74060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82381	WGS84	BB74060	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74060_Msal_Bot2012_COI	GA88	1	BB74060_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74060	Sample	BB74060	targeted	Absent	Sample	Msal_Bot2012_COI	GA88	COI	45	BB74060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA88	GYTAGTTCCRATGATGTTAGCTG	151.24304
BB74062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82186	WGS84	BB74062	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74062_Aamu_Bax2006_COI	GA86	1	BB74062_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74062	Sample	BB74062	targeted	Absent	Sample	Aamu_Bax2006_COI	GA86	COI	45	BB74062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA86	GCACAACCGGGATCTTTACTTC	151.24211
BB74062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82186	WGS84	BB74062	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74062_Pcan_Bot2011_IGS	GA86	1	BB74062_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74062	Sample	BB74062	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA86	IGS	45	BB74062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA86	CGTAATCCTCAGTACTGGCTA	151.24211
BB74062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82186	WGS84	BB74062	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74062_Sspa_Oph2007_COI	GA86	1	BB74062_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74062	Sample	BB74062	targeted	Absent	Sample	Sspa_Oph2007_COI	GA86	COI	45	BB74062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA86	GAAGGCCTTCCGATGATC	151.24211
BB74062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82186	WGS84	BB74062	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74062_Upin_Bot2011_COI	GA86	1	BB74062_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74062	Sample	BB74062	targeted	Absent	Sample	Upin_Bot2011_COI	GA86	COI	45	BB74062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA86	CTTTAATTACAGCGTTTTTATTGTTGT	151.24211
BB74063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8412	WGS84	BB74063	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74063_Mgig_Bot2012_COI	MA38	1	BB74063_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74063	Sample	BB74063	targeted	Absent	Sample	Mgig_Bot2012_COI	MA38	COI	45	BB74063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA38	TCTTATTCGTTGGAGACTTTATAACCCT	144.90371
BB74064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82104	WGS84	BB74064	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74064_Cmae_Bot2011_COI	GA83	1	BB74064_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74064	Sample	BB74064	targeted	Absent	Sample	Cmae_Bot2011_COI	GA83	COI	45	BB74064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA83	ATGAACAGTCTATCCTCCTTTAG	151.24161
BB74064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82104	WGS84	BB74064	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74064_Msal_Bot2012_COI	GA83	1	BB74064_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74064	Sample	BB74064	targeted	Absent	Sample	Msal_Bot2012_COI	GA83	COI	45	BB74064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA83	GYTAGTTCCRATGATGTTAGCTG	151.24161
BB74066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82005	WGS84	BB74066	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74066_Aamu_Bax2006_COI	GA81	1	BB74066_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74066	Sample	BB74066	targeted	Absent	Sample	Aamu_Bax2006_COI	GA81	COI	45	BB74066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA81	GCACAACCGGGATCTTTACTTC	151.24155
BB74066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82005	WGS84	BB74066	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74066_Pcan_Bot2011_IGS	GA81	1	BB74066_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74066	Sample	BB74066	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA81	IGS	45	BB74066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA81	CGTAATCCTCAGTACTGGCTA	151.24155
BB74066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82005	WGS84	BB74066	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74066_Sspa_Oph2007_COI	GA81	1	BB74066_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74066	Sample	BB74066	targeted	Absent	Sample	Sspa_Oph2007_COI	GA81	COI	45	BB74066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA81	GAAGGCCTTCCGATGATC	151.24155
BB74066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82005	WGS84	BB74066	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74066_Upin_Bot2011_COI	GA81	1	BB74066_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74066	Sample	BB74066	targeted	Absent	Sample	Upin_Bot2011_COI	GA81	COI	45	BB74066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA81	CTTTAATTACAGCGTTTTTATTGTTGT	151.24155
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.463	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74067_Mgig_Bot2012_COI	MA98	1	BB74067_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Present	Sample	Mgig_Bot2012_COI	MA98	COI	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	TCTTATTCGTTGGAGACTTTATAACCCT	144.92884
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74068_Cmae_Bot2011_COI	MA69	1	BB74068_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Absent	Sample	Cmae_Bot2011_COI	MA69	COI	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	ATGAACAGTCTATCCTCCTTTAG	144.90659
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74068_Hsan_Wil2021_COI	MA69	1	BB74068_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Absent	Sample	Hsan_Wil2021_COI	MA69	COI	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	CCTGGGCCGGTATAGTAGGT	144.90659
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74068_Msal_Bot2012_COI	MA69	1	BB74068_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Absent	Sample	Msal_Bot2012_COI	MA69	COI	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	GYTAGTTCCRATGATGTTAGCTG	144.90659
BB74070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82055	WGS84	BB74070	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74070_Mgig_Bot2012_COI	GA19	1	BB74070_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74070	Sample	BB74070	targeted	Absent	Sample	Mgig_Bot2012_COI	GA19	COI	45	BB74070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA19	TCTTATTCGTTGGAGACTTTATAACCCT	151.23966
BB74071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81913	WGS84	BB74071	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74071_Cmae_Bot2011_COI	GA18	1	BB74071_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74071	Sample	BB74071	targeted	Absent	Sample	Cmae_Bot2011_COI	GA18	COI	45	BB74071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA18	ATGAACAGTCTATCCTCCTTTAG	151.23931
BB74071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81913	WGS84	BB74071	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74071_Msal_Bot2012_COI	GA18	1	BB74071_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74071	Sample	BB74071	targeted	Absent	Sample	Msal_Bot2012_COI	GA18	COI	45	BB74071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA18	GYTAGTTCCRATGATGTTAGCTG	151.23931
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.311	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74073_Aamu_Bax2006_COI	MA54	1	BB74073_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Present	Sample	Aamu_Bax2006_COI	MA54	COI	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	GCACAACCGGGATCTTTACTTC	144.92822
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74073_Pcan_Bot2011_IGS	MA54	1	BB74073_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA54	IGS	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	CGTAATCCTCAGTACTGGCTA	144.92822
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.921	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74073_Sspa_Oph2007_COI	MA54	1	BB74073_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Present	Sample	Sspa_Oph2007_COI	MA54	COI	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	GAAGGCCTTCCGATGATC	144.92822
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74073_Upin_Bot2011_COI	MA54	1	BB74073_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Absent	Sample	Upin_Bot2011_COI	MA54	COI	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	CTTTAATTACAGCGTTTTTATTGTTGT	144.92822
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74074_Mgig_Bot2012_COI	MA64	1	BB74074_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Absent	Sample	Mgig_Bot2012_COI	MA64	COI	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	TCTTATTCGTTGGAGACTTTATAACCCT	144.92213
BB74075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81598	WGS84	BB74075	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74075_Cmae_Bot2011_COI	GA13	1	BB74075_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74075	Sample	BB74075	targeted	Absent	Sample	Cmae_Bot2011_COI	GA13	COI	45	BB74075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA13	ATGAACAGTCTATCCTCCTTTAG	151.23583
BB74075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81598	WGS84	BB74075	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74075_Msal_Bot2012_COI	GA13	1	BB74075_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74075	Sample	BB74075	targeted	Absent	Sample	Msal_Bot2012_COI	GA13	COI	45	BB74075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA13	GYTAGTTCCRATGATGTTAGCTG	151.23583
BB74077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83422	WGS84	BB74077	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74077_Aamu_Bax2006_COI	MA40	1	BB74077_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74077	Sample	BB74077	targeted	Absent	Sample	Aamu_Bax2006_COI	MA40	COI	45	BB74077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA40	GCACAACCGGGATCTTTACTTC	151.27119
BB74077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83422	WGS84	BB74077	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74077_Pcan_Bot2011_IGS	MA40	1	BB74077_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74077	Sample	BB74077	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA40	IGS	45	BB74077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA40	CGTAATCCTCAGTACTGGCTA	151.27119
BB74077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83422	WGS84	BB74077	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74077_Sspa_Oph2007_COI	MA40	1	BB74077_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74077	Sample	BB74077	targeted	Absent	Sample	Sspa_Oph2007_COI	MA40	COI	45	BB74077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA40	GAAGGCCTTCCGATGATC	151.27119
BB74077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83422	WGS84	BB74077	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74077_Upin_Bot2011_COI	MA40	1	BB74077_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74077	Sample	BB74077	targeted	Absent	Sample	Upin_Bot2011_COI	MA40	COI	45	BB74077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA40	CTTTAATTACAGCGTTTTTATTGTTGT	151.27119
BB74078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83245	WGS84	BB74078	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74078_Mgig_Bot2012_COI	GA78	1	BB74078_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74078	Sample	BB74078	targeted	Absent	Sample	Mgig_Bot2012_COI	GA78	COI	45	BB74078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA78	TCTTATTCGTTGGAGACTTTATAACCCT	151.26325
BB74079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83171	WGS84	BB74079	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74079_Cmae_Bot2011_COI	GA77	1	BB74079_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74079	Sample	BB74079	targeted	Absent	Sample	Cmae_Bot2011_COI	GA77	COI	45	BB74079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA77	ATGAACAGTCTATCCTCCTTTAG	151.262
BB74079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83171	WGS84	BB74079	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74079_Msal_Bot2012_COI	GA77	1	BB74079_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74079	Sample	BB74079	targeted	Absent	Sample	Msal_Bot2012_COI	GA77	COI	45	BB74079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA77	GYTAGTTCCRATGATGTTAGCTG	151.262
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.261	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74080_Aamu_Bax2006_COI	MA104	1	BB74080_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Present	Sample	Aamu_Bax2006_COI	MA104	COI	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	GCACAACCGGGATCTTTACTTC	144.90901
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74080_Pcan_Bot2011_IGS	MA104	1	BB74080_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA104	IGS	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	CGTAATCCTCAGTACTGGCTA	144.90901
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.367	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74080_Sspa_Oph2007_COI	MA104	1	BB74080_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Present	Sample	Sspa_Oph2007_COI	MA104	COI	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	GAAGGCCTTCCGATGATC	144.90901
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74080_Upin_Bot2011_COI	MA104	1	BB74080_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Absent	Sample	Upin_Bot2011_COI	MA104	COI	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	CTTTAATTACAGCGTTTTTATTGTTGT	144.90901
BB74081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83131	WGS84	BB74081	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74081_Mgig_Bot2012_COI	GA74	1	BB74081_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74081	Sample	BB74081	targeted	Absent	Sample	Mgig_Bot2012_COI	GA74	COI	45	BB74081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA74	TCTTATTCGTTGGAGACTTTATAACCCT	151.2555
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74082_Cmae_Bot2011_COI	MA106	1	BB74082_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Absent	Sample	Cmae_Bot2011_COI	MA106	COI	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	ATGAACAGTCTATCCTCCTTTAG	144.91161
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74082_Hsan_Wil2021_COI	MA106	1	BB74082_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Absent	Sample	Hsan_Wil2021_COI	MA106	COI	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	CCTGGGCCGGTATAGTAGGT	144.91161
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74082_Msal_Bot2012_COI	MA106	1	BB74082_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Absent	Sample	Msal_Bot2012_COI	MA106	COI	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	GYTAGTTCCRATGATGTTAGCTG	144.91161
BB74084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82352	WGS84	BB74084	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.745	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74084_Aamu_Bax2006_COI	MA107	1	BB74084_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74084	Sample	BB74084	targeted	Present	Sample	Aamu_Bax2006_COI	MA107	COI	45	BB74084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA107	GCACAACCGGGATCTTTACTTC	144.90269
BB74084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82352	WGS84	BB74084	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74084_Pcan_Bot2011_IGS	MA107	1	BB74084_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74084	Sample	BB74084	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA107	IGS	45	BB74084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA107	CGTAATCCTCAGTACTGGCTA	144.90269
BB74084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82352	WGS84	BB74084	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74084_Sspa_Oph2007_COI	MA107	1	BB74084_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74084	Sample	BB74084	targeted	Absent	Sample	Sspa_Oph2007_COI	MA107	COI	45	BB74084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA107	GAAGGCCTTCCGATGATC	144.90269
BB74084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82352	WGS84	BB74084	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74084_Upin_Bot2011_COI	MA107	1	BB74084_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74084	Sample	BB74084	targeted	Absent	Sample	Upin_Bot2011_COI	MA107	COI	45	BB74084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA107	CTTTAATTACAGCGTTTTTATTGTTGT	144.90269
BB74085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83556	WGS84	BB74085	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74085_Mgig_Bot2012_COI	GA41	1	BB74085_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74085	Sample	BB74085	targeted	Absent	Sample	Mgig_Bot2012_COI	GA41	COI	45	BB74085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA41	TCTTATTCGTTGGAGACTTTATAACCCT	151.27013
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74086_Cmae_Bot2011_COI	MA53	1	BB74086_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Absent	Sample	Cmae_Bot2011_COI	MA53	COI	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	ATGAACAGTCTATCCTCCTTTAG	144.92399
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74086_Hsan_Wil2021_COI	MA53	1	BB74086_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Absent	Sample	Hsan_Wil2021_COI	MA53	COI	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	CCTGGGCCGGTATAGTAGGT	144.92399
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74086_Msal_Bot2012_COI	MA53	1	BB74086_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Absent	Sample	Msal_Bot2012_COI	MA53	COI	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	GYTAGTTCCRATGATGTTAGCTG	144.92399
BB74088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83619	WGS84	BB74088	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74088_Aamu_Bax2006_COI	GA37	1	BB74088_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74088	Sample	BB74088	targeted	Absent	Sample	Aamu_Bax2006_COI	GA37	COI	45	BB74088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA37	GCACAACCGGGATCTTTACTTC	151.2719
BB74088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83619	WGS84	BB74088	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74088_Pcan_Bot2011_IGS	GA37	1	BB74088_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74088	Sample	BB74088	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA37	IGS	45	BB74088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA37	CGTAATCCTCAGTACTGGCTA	151.2719
BB74088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83619	WGS84	BB74088	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74088_Sspa_Oph2007_COI	GA37	1	BB74088_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74088	Sample	BB74088	targeted	Absent	Sample	Sspa_Oph2007_COI	GA37	COI	45	BB74088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA37	GAAGGCCTTCCGATGATC	151.2719
BB74088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83619	WGS84	BB74088	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74088_Upin_Bot2011_COI	GA37	1	BB74088_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74088	Sample	BB74088	targeted	Absent	Sample	Upin_Bot2011_COI	GA37	COI	45	BB74088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA37	CTTTAATTACAGCGTTTTTATTGTTGT	151.2719
BB74089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44344	WGS84	BB74089	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74089_Mgig_Bot2012_COI	BA2	1	BB74089_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74089	Sample	BB74089	targeted	Absent	Sample	Mgig_Bot2012_COI	BA2	COI	45	BB74089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA2	TCTTATTCGTTGGAGACTTTATAACCCT	153.07422
BB74187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85244	WGS84	BB74187	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74187_Aamu_Bax2006_COI	T1-01	1	BB74187_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74187	Sample	BB74187	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-01	COI	45	BB74187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-01	GCACAACCGGGATCTTTACTTC	147.86938
BB74187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85244	WGS84	BB74187	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74187_Pcan_Bot2011_IGS	T1-01	1	BB74187_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74187	Sample	BB74187	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-01	IGS	45	BB74187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-01	CGTAATCCTCAGTACTGGCTA	147.86938
BB74187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85244	WGS84	BB74187	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74187_Sspa_Oph2007_COI	T1-01	1	BB74187_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74187	Sample	BB74187	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-01	COI	45	BB74187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-01	GAAGGCCTTCCGATGATC	147.86938
BB74187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85244	WGS84	BB74187	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74187_Upin_Bot2011_COI	T1-01	1	BB74187_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74187	Sample	BB74187	targeted	Absent	Sample	Upin_Bot2011_COI	T1-01	COI	45	BB74187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.86938
BB74188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85433	WGS84	BB74188	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74188_Cjap_Sim2018_COI	T1-09	1	BB74188_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74188	Sample	BB74188	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-09	COI	45	BB74188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87329
BB74188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85433	WGS84	BB74188	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.164	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74188_Mgig_Bot2012_COI	T1-09	1	BB74188_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74188	Sample	BB74188	targeted	Present	Sample	Mgig_Bot2012_COI	T1-09	COI	45	BB74188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.87329
BB74189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85804	WGS84	BB74189	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74189_Cmae_Bot2011_COI	T1-17	1	BB74189_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74189	Sample	BB74189	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-17	COI	45	BB74189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-17	ATGAACAGTCTATCCTCCTTTAG	147.86795
BB74190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95973	WGS84	BB74190	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74190_Aamu_Bax2006_COI	T1-02	1	BB74190_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74190	Sample	BB74190	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-02	COI	45	BB74190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-02	GCACAACCGGGATCTTTACTTC	147.52492
BB74190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95973	WGS84	BB74190	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74190_Pcan_Bot2011_IGS	T1-02	1	BB74190_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74190	Sample	BB74190	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-02	IGS	45	BB74190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-02	CGTAATCCTCAGTACTGGCTA	147.52492
BB74190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95973	WGS84	BB74190	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74190_Sspa_Oph2007_COI	T1-02	1	BB74190_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74190	Sample	BB74190	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-02	COI	45	BB74190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-02	GAAGGCCTTCCGATGATC	147.52492
BB74190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95973	WGS84	BB74190	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74190_Upin_Bot2011_COI	T1-02	1	BB74190_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74190	Sample	BB74190	targeted	Absent	Sample	Upin_Bot2011_COI	T1-02	COI	45	BB74190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.52492
BB74191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96918	WGS84	BB74191	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74191_Cjap_Sim2018_COI	T1-10	1	BB74191_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74191	Sample	BB74191	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-10	COI	45	BB74191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51603
BB74191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96918	WGS84	BB74191	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.252	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74191_Mgig_Bot2012_COI	T1-10	1	BB74191_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74191	Sample	BB74191	targeted	Present	Sample	Mgig_Bot2012_COI	T1-10	COI	45	BB74191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.51603
BB74192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96019	WGS84	BB74192	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.783	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74192_Cmae_Bot2011_COI	T1-18	1	BB74192_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74192	Sample	BB74192	targeted	Present	Sample	Cmae_Bot2011_COI	T1-18	COI	45	BB74192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-18	ATGAACAGTCTATCCTCCTTTAG	147.52897
BB74194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88441	WGS84	BB74194	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74194_Aamu_Bax2006_COI	T1-11	1	BB74194_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74194	Sample	BB74194	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-11	COI	45	BB74194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-11	GCACAACCGGGATCTTTACTTC	147.33665
BB74194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88441	WGS84	BB74194	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74194_Pcan_Bot2011_IGS	T1-11	1	BB74194_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74194	Sample	BB74194	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-11	IGS	45	BB74194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-11	CGTAATCCTCAGTACTGGCTA	147.33665
BB74194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88441	WGS84	BB74194	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74194_Sspa_Oph2007_COI	T1-11	1	BB74194_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74194	Sample	BB74194	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-11	COI	45	BB74194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-11	GAAGGCCTTCCGATGATC	147.33665
BB74194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88441	WGS84	BB74194	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74194_Upin_Bot2011_COI	T1-11	1	BB74194_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74194	Sample	BB74194	targeted	Absent	Sample	Upin_Bot2011_COI	T1-11	COI	45	BB74194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33665
BB74195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88393	WGS84	BB74195	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74195_Cjap_Sim2018_COI	T1-19	1	BB74195_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74195	Sample	BB74195	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-19	COI	45	BB74195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33802
BB74195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88393	WGS84	BB74195	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.767	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74195_Mgig_Bot2012_COI	T1-19	1	BB74195_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74195	Sample	BB74195	targeted	Present	Sample	Mgig_Bot2012_COI	T1-19	COI	45	BB74195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-19	TCTTATTCGTTGGAGACTTTATAACCCT	147.33802
BB74196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87764	WGS84	BB74196	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74196_Cmae_Bot2011_COI	T1-04	1	BB74196_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74196	Sample	BB74196	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-04	COI	45	BB74196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-04	ATGAACAGTCTATCCTCCTTTAG	147.34464
BB74198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82426	WGS84	BB74198	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74198_Aamu_Bax2006_COI	T1-20	1	BB74198_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74198	Sample	BB74198	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-20	COI	45	BB74198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-20	GCACAACCGGGATCTTTACTTC	147.30787
BB74198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82426	WGS84	BB74198	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74198_Pcan_Bot2011_IGS	T1-20	1	BB74198_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74198	Sample	BB74198	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-20	IGS	45	BB74198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-20	CGTAATCCTCAGTACTGGCTA	147.30787
BB74198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82426	WGS84	BB74198	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74198_Sspa_Oph2007_COI	T1-20	1	BB74198_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74198	Sample	BB74198	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-20	COI	45	BB74198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-20	GAAGGCCTTCCGATGATC	147.30787
BB74198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82426	WGS84	BB74198	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74198_Upin_Bot2011_COI	T1-20	1	BB74198_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74198	Sample	BB74198	targeted	Absent	Sample	Upin_Bot2011_COI	T1-20	COI	45	BB74198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-20	CTTTAATTACAGCGTTTTTATTGTTGT	147.30787
BB74199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84579	WGS84	BB74199	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74199_Cjap_Sim2018_COI	S2-03	1	BB74199_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74199	Sample	BB74199	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-03	COI	45	BB74199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49682
BB74199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84579	WGS84	BB74199	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74199_Mgig_Bot2012_COI	S2-03	1	BB74199_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74199	Sample	BB74199	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-03	COI	45	BB74199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.49682
BB74200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84483	WGS84	BB74200	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74200_Cmae_Bot2011_COI	S2-05	1	BB74200_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74200	Sample	BB74200	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-05	COI	45	BB74200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-05	ATGAACAGTCTATCCTCCTTTAG	138.49685
BB74202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78745	WGS84	BB74202	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74202_Aamu_Bax2006_COI	S2-11	1	BB74202_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74202	Sample	BB74202	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-11	COI	45	BB74202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-11	GCACAACCGGGATCTTTACTTC	138.51416
BB74202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78745	WGS84	BB74202	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74202_Pcan_Bot2011_IGS	S2-11	1	BB74202_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74202	Sample	BB74202	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-11	IGS	45	BB74202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-11	CGTAATCCTCAGTACTGGCTA	138.51416
BB74202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78745	WGS84	BB74202	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74202_Sspa_Oph2007_COI	S2-11	1	BB74202_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74202	Sample	BB74202	targeted	Absent	Sample	Sspa_Oph2007_COI	S2-11	COI	45	BB74202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-11	GAAGGCCTTCCGATGATC	138.51416
BB74202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78745	WGS84	BB74202	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74202_Upin_Bot2011_COI	S2-11	1	BB74202_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74202	Sample	BB74202	targeted	Absent	Sample	Upin_Bot2011_COI	S2-11	COI	45	BB74202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.51416
BB74203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83626	WGS84	BB74203	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74203_Cjap_Sim2018_COI	S2-10	1	BB74203_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74203	Sample	BB74203	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-10	COI	45	BB74203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50837
BB74203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83626	WGS84	BB74203	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74203_Mgig_Bot2012_COI	S2-10	1	BB74203_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74203	Sample	BB74203	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-10	COI	45	BB74203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.50837
BB74204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78612	WGS84	BB74204	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74204_Cmae_Bot2011_COI	S2-13	1	BB74204_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74204	Sample	BB74204	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-13	COI	45	BB74204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-13	ATGAACAGTCTATCCTCCTTTAG	138.51493
BB74206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78616	WGS84	BB74206	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74206_Aamu_Bax2006_COI	S2-14	1	BB74206_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74206	Sample	BB74206	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-14	COI	45	BB74206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-14	GCACAACCGGGATCTTTACTTC	138.51483
BB74206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78616	WGS84	BB74206	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74206_Pcan_Bot2011_IGS	S2-14	1	BB74206_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74206	Sample	BB74206	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-14	IGS	45	BB74206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-14	CGTAATCCTCAGTACTGGCTA	138.51483
BB74206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78616	WGS84	BB74206	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.355	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74206_Sspa_Oph2007_COI	S2-14	1	BB74206_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74206	Sample	BB74206	targeted	Present	Sample	Sspa_Oph2007_COI	S2-14	COI	45	BB74206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-14	GAAGGCCTTCCGATGATC	138.51483
BB74206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78616	WGS84	BB74206	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74206_Upin_Bot2011_COI	S2-14	1	BB74206_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74206	Sample	BB74206	targeted	Absent	Sample	Upin_Bot2011_COI	S2-14	COI	45	BB74206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.51483
BB75216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78716	WGS84	BB75216	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75216_Cmae_Bot2011_COI	S2-15	1	BB75216_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75216	Sample	BB75216	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-15	COI	45	BB75216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-15	ATGAACAGTCTATCCTCCTTTAG	138.51476
BB75218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80908	WGS84	BB75218	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75218_Aamu_Bax2006_COI	S2-17	1	BB75218_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75218	Sample	BB75218	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-17	COI	45	BB75218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-17	GCACAACCGGGATCTTTACTTC	138.51763
BB75218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80908	WGS84	BB75218	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75218_Pcan_Bot2011_IGS	S2-17	1	BB75218_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75218	Sample	BB75218	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-17	IGS	45	BB75218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-17	CGTAATCCTCAGTACTGGCTA	138.51763
BB75218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80908	WGS84	BB75218	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.889	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75218_Sspa_Oph2007_COI	S2-17	1	BB75218_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75218	Sample	BB75218	targeted	Present	Sample	Sspa_Oph2007_COI	S2-17	COI	45	BB75218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-17	GAAGGCCTTCCGATGATC	138.51763
BB75218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80908	WGS84	BB75218	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75218_Upin_Bot2011_COI	S2-17	1	BB75218_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75218	Sample	BB75218	targeted	Absent	Sample	Upin_Bot2011_COI	S2-17	COI	45	BB75218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.51763
BB75219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80944	WGS84	BB75219	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75219_Cjap_Sim2018_COI	S2-18	1	BB75219_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75219	Sample	BB75219	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-18	COI	45	BB75219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51875
BB75219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80944	WGS84	BB75219	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75219_Mgig_Bot2012_COI	S2-18	1	BB75219_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75219	Sample	BB75219	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-18	COI	45	BB75219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.51875
BB75221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85207	WGS84	BB75221	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75221_Aamu_Bax2006_COI	T1-05	1	BB75221_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75221	Sample	BB75221	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-05	COI	45	BB75221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-05	GCACAACCGGGATCTTTACTTC	147.87165
BB75221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85207	WGS84	BB75221	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75221_Pcan_Bot2011_IGS	T1-05	1	BB75221_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75221	Sample	BB75221	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-05	IGS	45	BB75221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-05	CGTAATCCTCAGTACTGGCTA	147.87165
BB75221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85207	WGS84	BB75221	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75221_Sspa_Oph2007_COI	T1-05	1	BB75221_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75221	Sample	BB75221	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-05	COI	45	BB75221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-05	GAAGGCCTTCCGATGATC	147.87165
BB75221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85207	WGS84	BB75221	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75221_Upin_Bot2011_COI	T1-05	1	BB75221_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75221	Sample	BB75221	targeted	Absent	Sample	Upin_Bot2011_COI	T1-05	COI	45	BB75221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.87165
BB75222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85712	WGS84	BB75222	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75222_Cjap_Sim2018_COI	T1-13	1	BB75222_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75222	Sample	BB75222	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-13	COI	45	BB75222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87617
BB75222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85712	WGS84	BB75222	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.792	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75222_Mgig_Bot2012_COI	T1-13	1	BB75222_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75222	Sample	BB75222	targeted	Present	Sample	Mgig_Bot2012_COI	T1-13	COI	45	BB75222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.87617
BB75223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96422	WGS84	BB75223	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.416	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75223_Cmae_Bot2011_COI	T1-06	1	BB75223_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75223	Sample	BB75223	targeted	Present	Sample	Cmae_Bot2011_COI	T1-06	COI	45	BB75223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-06	ATGAACAGTCTATCCTCCTTTAG	147.51968
BB75225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88573	WGS84	BB75225	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75225_Aamu_Bax2006_COI	T1-07	1	BB75225_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75225	Sample	BB75225	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-07	COI	45	BB75225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-07	GCACAACCGGGATCTTTACTTC	147.33828
BB75225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88573	WGS84	BB75225	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75225_Pcan_Bot2011_IGS	T1-07	1	BB75225_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75225	Sample	BB75225	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-07	IGS	45	BB75225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-07	CGTAATCCTCAGTACTGGCTA	147.33828
BB75225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88573	WGS84	BB75225	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75225_Sspa_Oph2007_COI	T1-07	1	BB75225_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75225	Sample	BB75225	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-07	COI	45	BB75225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-07	GAAGGCCTTCCGATGATC	147.33828
BB75225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88573	WGS84	BB75225	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75225_Upin_Bot2011_COI	T1-07	1	BB75225_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75225	Sample	BB75225	targeted	Absent	Sample	Upin_Bot2011_COI	T1-07	COI	45	BB75225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.33828
BB75226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87458	WGS84	BB75226	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75226_Cjap_Sim2018_COI	T1-15	1	BB75226_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75226	Sample	BB75226	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-15	COI	45	BB75226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33635
BB75226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87458	WGS84	BB75226	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.44	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75226_Mgig_Bot2012_COI	T1-15	1	BB75226_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75226	Sample	BB75226	targeted	Present	Sample	Mgig_Bot2012_COI	T1-15	COI	45	BB75226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.33635
BB75227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87996	WGS84	BB75227	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	41.133	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75227_Cmae_Bot2011_COI	T1-08	1	BB75227_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75227	Sample	BB75227	targeted	Present	Sample	Cmae_Bot2011_COI	T1-08	COI	45	BB75227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-08	ATGAACAGTCTATCCTCCTTTAG	147.34157
BB75229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82712	WGS84	BB75229	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.299	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75229_Aamu_Bax2006_COI	T4-02	1	BB75229_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75229	Sample	BB75229	targeted	Present	Sample	Aamu_Bax2006_COI	T4-02	COI	45	BB75229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-02	GCACAACCGGGATCTTTACTTC	147.3019
BB75229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82712	WGS84	BB75229	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75229_Pcan_Bot2011_IGS	T4-02	1	BB75229_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75229	Sample	BB75229	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-02	IGS	45	BB75229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-02	CGTAATCCTCAGTACTGGCTA	147.3019
BB75229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82712	WGS84	BB75229	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75229_Sspa_Oph2007_COI	T4-02	1	BB75229_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75229	Sample	BB75229	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-02	COI	45	BB75229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-02	GAAGGCCTTCCGATGATC	147.3019
BB75229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82712	WGS84	BB75229	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75229_Upin_Bot2011_COI	T4-02	1	BB75229_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75229	Sample	BB75229	targeted	Absent	Sample	Upin_Bot2011_COI	T4-02	COI	45	BB75229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.3019
BB75230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87299	WGS84	BB75230	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.745	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75230_Cmae_Bot2011_COI	T4-04	1	BB75230_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75230	Sample	BB75230	targeted	Present	Sample	Cmae_Bot2011_COI	T4-04	COI	45	BB75230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-04	ATGAACAGTCTATCCTCCTTTAG	147.33679
BB75232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89955	WGS84	BB75232	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.977	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75232_Aamu_Bax2006_COI	T4-08	1	BB75232_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75232	Sample	BB75232	targeted	Present	Sample	Aamu_Bax2006_COI	T4-08	COI	45	BB75232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-08	GCACAACCGGGATCTTTACTTC	147.33941
BB75232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89955	WGS84	BB75232	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75232_Pcan_Bot2011_IGS	T4-08	1	BB75232_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75232	Sample	BB75232	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-08	IGS	45	BB75232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-08	CGTAATCCTCAGTACTGGCTA	147.33941
BB75232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89955	WGS84	BB75232	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75232_Sspa_Oph2007_COI	T4-08	1	BB75232_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75232	Sample	BB75232	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-08	COI	45	BB75232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-08	GAAGGCCTTCCGATGATC	147.33941
BB75232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89955	WGS84	BB75232	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75232_Upin_Bot2011_COI	T4-08	1	BB75232_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75232	Sample	BB75232	targeted	Absent	Sample	Upin_Bot2011_COI	T4-08	COI	45	BB75232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.33941
BB75233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84798	WGS84	BB75233	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75233_Cjap_Sim2018_COI	T4-10	1	BB75233_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75233	Sample	BB75233	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-10	COI	45	BB75233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87403
BB75233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.84798	WGS84	BB75233	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75233_Mgig_Bot2012_COI	T4-10	1	BB75233_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75233	Sample	BB75233	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-10	COI	45	BB75233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.87403
BB75234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85713	WGS84	BB75234	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75234_Cmae_Bot2011_COI	T4-12	1	BB75234_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75234	Sample	BB75234	targeted	Absent	Sample	Cmae_Bot2011_COI	T4-12	COI	45	BB75234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-12	ATGAACAGTCTATCCTCCTTTAG	147.87276
BB75236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82928	WGS84	BB75236	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.31	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75236_Aamu_Bax2006_COI	T4-01	1	BB75236_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75236	Sample	BB75236	targeted	Present	Sample	Aamu_Bax2006_COI	T4-01	COI	45	BB75236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-01	GCACAACCGGGATCTTTACTTC	147.30265
BB75236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82928	WGS84	BB75236	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75236_Pcan_Bot2011_IGS	T4-01	1	BB75236_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75236	Sample	BB75236	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-01	IGS	45	BB75236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-01	CGTAATCCTCAGTACTGGCTA	147.30265
BB75236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82928	WGS84	BB75236	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75236_Sspa_Oph2007_COI	T4-01	1	BB75236_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75236	Sample	BB75236	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-01	COI	45	BB75236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-01	GAAGGCCTTCCGATGATC	147.30265
BB75236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82928	WGS84	BB75236	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75236_Upin_Bot2011_COI	T4-01	1	BB75236_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75236	Sample	BB75236	targeted	Absent	Sample	Upin_Bot2011_COI	T4-01	COI	45	BB75236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.30265
BB75237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82524	WGS84	BB75237	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75237_Cjap_Sim2018_COI	T4-03	1	BB75237_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75237	Sample	BB75237	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-03	COI	45	BB75237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30752
BB75237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82524	WGS84	BB75237	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75237_Mgig_Bot2012_COI	T4-03	1	BB75237_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75237	Sample	BB75237	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-03	COI	45	BB75237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.30752
BB75238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87854	WGS84	BB75238	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.376	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75238_Cmae_Bot2011_COI	T4-05	1	BB75238_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75238	Sample	BB75238	targeted	Present	Sample	Cmae_Bot2011_COI	T4-05	COI	45	BB75238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-05	ATGAACAGTCTATCCTCCTTTAG	147.34183
BB75240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88548	WGS84	BB75240	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75240_Cjap_Sim2018_COI	T4-09	1	BB75240_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75240	Sample	BB75240	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-09	COI	45	BB75240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33615
BB75240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88548	WGS84	BB75240	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75240_Mgig_Bot2012_COI	T4-09	1	BB75240_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75240	Sample	BB75240	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-09	COI	45	BB75240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.33615
BB75241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.8512	WGS84	BB75241	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.09	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75241_Cmae_Bot2011_COI	T4-11	1	BB75241_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75241	Sample	BB75241	targeted	Present	Sample	Cmae_Bot2011_COI	T4-11	COI	45	BB75241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-11	ATGAACAGTCTATCCTCCTTTAG	147.87357
BB75243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96162	WGS84	BB75243	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.37	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75243_Aamu_Bax2006_COI	T4-15	1	BB75243_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75243	Sample	BB75243	targeted	Present	Sample	Aamu_Bax2006_COI	T4-15	COI	45	BB75243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-15	GCACAACCGGGATCTTTACTTC	147.51952
BB75243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96162	WGS84	BB75243	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75243_Pcan_Bot2011_IGS	T4-15	1	BB75243_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75243	Sample	BB75243	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-15	IGS	45	BB75243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-15	CGTAATCCTCAGTACTGGCTA	147.51952
BB75243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96162	WGS84	BB75243	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75243_Sspa_Oph2007_COI	T4-15	1	BB75243_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75243	Sample	BB75243	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-15	COI	45	BB75243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-15	GAAGGCCTTCCGATGATC	147.51952
BB75243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96162	WGS84	BB75243	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75243_Upin_Bot2011_COI	T4-15	1	BB75243_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75243	Sample	BB75243	targeted	Absent	Sample	Upin_Bot2011_COI	T4-15	COI	45	BB75243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.51952
BB75247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81044	WGS84	BB75247	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75247_Aamu_Bax2006_COI	S1-20	1	BB75247_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75247	Sample	BB75247	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-20	COI	45	BB75247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-20	GCACAACCGGGATCTTTACTTC	138.52108
BB75247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81044	WGS84	BB75247	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75247_Pcan_Bot2011_IGS	S1-20	1	BB75247_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75247	Sample	BB75247	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-20	IGS	45	BB75247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-20	CGTAATCCTCAGTACTGGCTA	138.52108
BB75247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81044	WGS84	BB75247	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75247_Sspa_Oph2007_COI	S1-20	1	BB75247_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75247	Sample	BB75247	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-20	COI	45	BB75247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-20	GAAGGCCTTCCGATGATC	138.52108
BB75247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81044	WGS84	BB75247	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75247_Upin_Bot2011_COI	S1-20	1	BB75247_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75247	Sample	BB75247	targeted	Absent	Sample	Upin_Bot2011_COI	S1-20	COI	45	BB75247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-20	CTTTAATTACAGCGTTTTTATTGTTGT	138.52108
BB75248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80931	WGS84	BB75248	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75248_Cjap_Sim2018_COI	S2-20	1	BB75248_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75248	Sample	BB75248	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-20	COI	45	BB75248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-20	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5177
BB75248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80931	WGS84	BB75248	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75248_Mgig_Bot2012_COI	S2-20	1	BB75248_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75248	Sample	BB75248	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-20	COI	45	BB75248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-20	TCTTATTCGTTGGAGACTTTATAACCCT	138.5177
BB80076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.7824	WGS84	BB80076	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80076_Aamu_Bax2006_COI	S4-01	1	BB80076_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80076	Sample	BB80076	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-01	COI	45	BB80076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-01	GCACAACCGGGATCTTTACTTC	138.51709
BB80076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.7824	WGS84	BB80076	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80076_Pcan_Bot2011_IGS	S4-01	1	BB80076_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80076	Sample	BB80076	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-01	IGS	45	BB80076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-01	CGTAATCCTCAGTACTGGCTA	138.51709
BB80076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.7824	WGS84	BB80076	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80076_Sspa_Oph2007_COI	S4-01	1	BB80076_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80076	Sample	BB80076	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-01	COI	45	BB80076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-01	GAAGGCCTTCCGATGATC	138.51709
BB80076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.7824	WGS84	BB80076	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80076_Upin_Bot2011_COI	S4-01	1	BB80076_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80076	Sample	BB80076	targeted	Absent	Sample	Upin_Bot2011_COI	S4-01	COI	45	BB80076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.51709
BB80077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78295	WGS84	BB80077	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80077_Cjap_Sim2018_COI	S4-02	1	BB80077_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80077	Sample	BB80077	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-02	COI	45	BB80077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51697
BB80077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78295	WGS84	BB80077	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.926	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80077_Mgig_Bot2012_COI	S4-02	1	BB80077_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80077	Sample	BB80077	targeted	Present	Sample	Mgig_Bot2012_COI	S4-02	COI	45	BB80077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.51697
BB80078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78422	WGS84	BB80078	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80078_Cmae_Bot2011_COI	S4-03	1	BB80078_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80078	Sample	BB80078	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-03	COI	45	BB80078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-03	ATGAACAGTCTATCCTCCTTTAG	138.51657
BB80080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78646	WGS84	BB80080	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.325	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80080_Mgig_Bot2012_COI	S4-05	1	BB80080_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80080	Sample	BB80080	targeted	Present	Sample	Mgig_Bot2012_COI	S4-05	COI	45	BB80080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.51561
BB80081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84367	WGS84	BB80081	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80081_Cmae_Bot2011_COI	S4-06	1	BB80081_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80081	Sample	BB80081	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-06	COI	45	BB80081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-06	ATGAACAGTCTATCCTCCTTTAG	138.49764
BB80084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84516	WGS84	BB80084	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80084_Cjap_Sim2018_COI	S4-08	1	BB80084_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80084	Sample	BB80084	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-08	COI	45	BB80084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49722
BB80084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84516	WGS84	BB80084	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.117	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80084_Mgig_Bot2012_COI	S4-08	1	BB80084_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80084	Sample	BB80084	targeted	Present	Sample	Mgig_Bot2012_COI	S4-08	COI	45	BB80084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.49722
BB80087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83727	WGS84	BB80087	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80087_Aamu_Bax2006_COI	S4-11	1	BB80087_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80087	Sample	BB80087	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-11	COI	45	BB80087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-11	GCACAACCGGGATCTTTACTTC	138.50732
BB80087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83727	WGS84	BB80087	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80087_Pcan_Bot2011_IGS	S4-11	1	BB80087_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80087	Sample	BB80087	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-11	IGS	45	BB80087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-11	CGTAATCCTCAGTACTGGCTA	138.50732
BB80087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83727	WGS84	BB80087	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80087_Sspa_Oph2007_COI	S4-11	1	BB80087_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80087	Sample	BB80087	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-11	COI	45	BB80087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-11	GAAGGCCTTCCGATGATC	138.50732
BB80087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83727	WGS84	BB80087	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80087_Upin_Bot2011_COI	S4-11	1	BB80087_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80087	Sample	BB80087	targeted	Absent	Sample	Upin_Bot2011_COI	S4-11	COI	45	BB80087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.50732
BB80088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8367	WGS84	BB80088	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80088_Cjap_Sim2018_COI	S4-12	1	BB80088_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80088	Sample	BB80088	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-12	COI	45	BB80088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50862
BB80088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.8367	WGS84	BB80088	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.286	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80088_Mgig_Bot2012_COI	S4-12	1	BB80088_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80088	Sample	BB80088	targeted	Present	Sample	Mgig_Bot2012_COI	S4-12	COI	45	BB80088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.50862
BB80089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83697	WGS84	BB80089	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80089_Cmae_Bot2011_COI	S4-13	1	BB80089_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80089	Sample	BB80089	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-13	COI	45	BB80089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-13	ATGAACAGTCTATCCTCCTTTAG	138.50959
BB80106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78368	WGS84	BB80106	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80106_Aamu_Bax2006_COI	S3-20	1	BB80106_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80106	Sample	BB80106	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-20	COI	45	BB80106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-20	GCACAACCGGGATCTTTACTTC	138.5164
BB80106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78368	WGS84	BB80106	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80106_Pcan_Bot2011_IGS	S3-20	1	BB80106_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80106	Sample	BB80106	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-20	IGS	45	BB80106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-20	CGTAATCCTCAGTACTGGCTA	138.5164
BB80106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78368	WGS84	BB80106	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80106_Sspa_Oph2007_COI	S3-20	1	BB80106_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80106	Sample	BB80106	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-20	COI	45	BB80106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-20	GAAGGCCTTCCGATGATC	138.5164
BB80106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78368	WGS84	BB80106	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80106_Upin_Bot2011_COI	S3-20	1	BB80106_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80106	Sample	BB80106	targeted	Absent	Sample	Upin_Bot2011_COI	S3-20	COI	45	BB80106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-20	CTTTAATTACAGCGTTTTTATTGTTGT	138.5164
BB80107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78497	WGS84	BB80107	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.876	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80107_Mgig_Bot2012_COI	S3-19	1	BB80107_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80107	Sample	BB80107	targeted	Present	Sample	Mgig_Bot2012_COI	S3-19	COI	45	BB80107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.51619
BB80108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78601	WGS84	BB80108	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80108_Cmae_Bot2011_COI	S3-18	1	BB80108_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80108	Sample	BB80108	targeted	Absent	Sample	Cmae_Bot2011_COI	S3-18	COI	45	BB80108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-18	ATGAACAGTCTATCCTCCTTTAG	138.51598
BB80110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78837	WGS84	BB80110	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80110_Cjap_Sim2018_COI	S3-16	1	BB80110_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80110	Sample	BB80110	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-16	COI	45	BB80110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51486
BB80110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78837	WGS84	BB80110	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.88	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80110_Mgig_Bot2012_COI	S3-16	1	BB80110_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80110	Sample	BB80110	targeted	Present	Sample	Mgig_Bot2012_COI	S3-16	COI	45	BB80110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.51486
BB80111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81136	WGS84	BB80111	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.828	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80111_Cmae_Bot2011_COI	S3-15	1	BB80111_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80111	Sample	BB80111	targeted	Present	Sample	Cmae_Bot2011_COI	S3-15	COI	45	BB80111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-15	ATGAACAGTCTATCCTCCTTTAG	138.52303
BB80113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81015	WGS84	BB80113	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80113_Aamu_Bax2006_COI	S3-13	1	BB80113_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80113	Sample	BB80113	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-13	COI	45	BB80113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-13	GCACAACCGGGATCTTTACTTC	138.52006
BB80113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81015	WGS84	BB80113	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80113_Pcan_Bot2011_IGS	S3-13	1	BB80113_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80113	Sample	BB80113	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-13	IGS	45	BB80113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-13	CGTAATCCTCAGTACTGGCTA	138.52006
BB80113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81015	WGS84	BB80113	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80113_Sspa_Oph2007_COI	S3-13	1	BB80113_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80113	Sample	BB80113	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-13	COI	45	BB80113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-13	GAAGGCCTTCCGATGATC	138.52006
BB80113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81015	WGS84	BB80113	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80113_Upin_Bot2011_COI	S3-13	1	BB80113_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80113	Sample	BB80113	targeted	Absent	Sample	Upin_Bot2011_COI	S3-13	COI	45	BB80113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.52006
BB80114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80965	WGS84	BB80114	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80114_Cjap_Sim2018_COI	S3-12	1	BB80114_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80114	Sample	BB80114	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-12	COI	45	BB80114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51882
BB80114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80965	WGS84	BB80114	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.937	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80114_Mgig_Bot2012_COI	S3-12	1	BB80114_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80114	Sample	BB80114	targeted	Present	Sample	Mgig_Bot2012_COI	S3-12	COI	45	BB80114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.51882
BB80115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80903	WGS84	BB80115	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.247	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80115_Cmae_Bot2011_COI	S3-11	1	BB80115_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80115	Sample	BB80115	targeted	Present	Sample	Cmae_Bot2011_COI	S3-11	COI	45	BB80115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-11	ATGAACAGTCTATCCTCCTTTAG	138.51747
BB80117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83634	WGS84	BB80117	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80117_Aamu_Bax2006_COI	S3-09	1	BB80117_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80117	Sample	BB80117	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-09	COI	45	BB80117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-09	GCACAACCGGGATCTTTACTTC	138.5092
BB80117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83634	WGS84	BB80117	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80117_Pcan_Bot2011_IGS	S3-09	1	BB80117_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80117	Sample	BB80117	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-09	IGS	45	BB80117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-09	CGTAATCCTCAGTACTGGCTA	138.5092
BB80117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83634	WGS84	BB80117	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80117_Sspa_Oph2007_COI	S3-09	1	BB80117_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80117	Sample	BB80117	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-09	COI	45	BB80117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-09	GAAGGCCTTCCGATGATC	138.5092
BB80117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83634	WGS84	BB80117	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80117_Upin_Bot2011_COI	S3-09	1	BB80117_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80117	Sample	BB80117	targeted	Absent	Sample	Upin_Bot2011_COI	S3-09	COI	45	BB80117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.5092
BB80118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83573	WGS84	BB80118	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80118_Cjap_Sim2018_COI	S3-10	1	BB80118_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80118	Sample	BB80118	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-10	COI	45	BB80118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50796
BB80118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83573	WGS84	BB80118	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.601	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80118_Mgig_Bot2012_COI	S3-10	1	BB80118_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80118	Sample	BB80118	targeted	Present	Sample	Mgig_Bot2012_COI	S3-10	COI	45	BB80118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.50796
BB80119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83679	WGS84	BB80119	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.267	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80119_Cmae_Bot2011_COI	S3-07	1	BB80119_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80119	Sample	BB80119	targeted	Present	Sample	Cmae_Bot2011_COI	S3-07	COI	45	BB80119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-07	ATGAACAGTCTATCCTCCTTTAG	138.50733
BB80120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83819	WGS84	BB80120	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80120_Aamu_Bax2006_COI	S3-06	1	BB80120_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80120	Sample	BB80120	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-06	COI	45	BB80120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-06	GCACAACCGGGATCTTTACTTC	138.50691
BB80120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83819	WGS84	BB80120	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80120_Pcan_Bot2011_IGS	S3-06	1	BB80120_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80120	Sample	BB80120	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-06	IGS	45	BB80120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-06	CGTAATCCTCAGTACTGGCTA	138.50691
BB80120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83819	WGS84	BB80120	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80120_Sspa_Oph2007_COI	S3-06	1	BB80120_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80120	Sample	BB80120	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-06	COI	45	BB80120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-06	GAAGGCCTTCCGATGATC	138.50691
BB80120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83819	WGS84	BB80120	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80120_Upin_Bot2011_COI	S3-06	1	BB80120_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80120	Sample	BB80120	targeted	Absent	Sample	Upin_Bot2011_COI	S3-06	COI	45	BB80120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.50691
BB80121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84305	WGS84	BB80121	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80121_Cjap_Sim2018_COI	S3-05	1	BB80121_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80121	Sample	BB80121	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-05	COI	45	BB80121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49947
BB80121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84305	WGS84	BB80121	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.254	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80121_Mgig_Bot2012_COI	S3-05	1	BB80121_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80121	Sample	BB80121	targeted	Present	Sample	Mgig_Bot2012_COI	S3-05	COI	45	BB80121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.49947
BB80122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84355	WGS84	BB80122	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.045	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80122_Cmae_Bot2011_COI	S3-04	1	BB80122_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80122	Sample	BB80122	targeted	Present	Sample	Cmae_Bot2011_COI	S3-04	COI	45	BB80122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-04	ATGAACAGTCTATCCTCCTTTAG	138.49824
BB80124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8452	WGS84	BB80124	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80124_Aamu_Bax2006_COI	S3-02	1	BB80124_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80124	Sample	BB80124	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-02	COI	45	BB80124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-02	GCACAACCGGGATCTTTACTTC	138.4972
BB80124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8452	WGS84	BB80124	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80124_Pcan_Bot2011_IGS	S3-02	1	BB80124_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80124	Sample	BB80124	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-02	IGS	45	BB80124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-02	CGTAATCCTCAGTACTGGCTA	138.4972
BB80124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8452	WGS84	BB80124	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80124_Sspa_Oph2007_COI	S3-02	1	BB80124_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80124	Sample	BB80124	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-02	COI	45	BB80124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-02	GAAGGCCTTCCGATGATC	138.4972
BB80124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8452	WGS84	BB80124	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80124_Upin_Bot2011_COI	S3-02	1	BB80124_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80124	Sample	BB80124	targeted	Absent	Sample	Upin_Bot2011_COI	S3-02	COI	45	BB80124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.4972
BB80125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84665	WGS84	BB80125	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80125_Cjap_Sim2018_COI	S3-01	1	BB80125_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80125	Sample	BB80125	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-01	COI	45	BB80125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49724
BB80125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84665	WGS84	BB80125	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.612	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80125_Mgig_Bot2012_COI	S3-01	1	BB80125_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80125	Sample	BB80125	targeted	Present	Sample	Mgig_Bot2012_COI	S3-01	COI	45	BB80125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.49724
BB80146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35841	WGS84	BB80146	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80146_Aamu_Bax2006_COI	BB2	1	BB80146_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80146	Sample	BB80146	targeted	Absent	Sample	Aamu_Bax2006_COI	BB2	COI	45	BB80146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB2	GCACAACCGGGATCTTTACTTC	153.17941
BB80146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35841	WGS84	BB80146	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80146_Pcan_Bot2011_IGS	BB2	1	BB80146_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80146	Sample	BB80146	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB2	IGS	45	BB80146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB2	CGTAATCCTCAGTACTGGCTA	153.17941
BB80146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35841	WGS84	BB80146	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80146_Sspa_Oph2007_COI	BB2	1	BB80146_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80146	Sample	BB80146	targeted	Absent	Sample	Sspa_Oph2007_COI	BB2	COI	45	BB80146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB2	GAAGGCCTTCCGATGATC	153.17941
BB80146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35841	WGS84	BB80146	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80146_Upin_Bot2011_COI	BB2	1	BB80146_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80146	Sample	BB80146	targeted	Absent	Sample	Upin_Bot2011_COI	BB2	COI	45	BB80146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB2	CTTTAATTACAGCGTTTTTATTGTTGT	153.17941
BB80147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38249	WGS84	BB80147	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80147_Mgig_Bot2012_COI	BB18	1	BB80147_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80147	Sample	BB80147	targeted	Absent	Sample	Mgig_Bot2012_COI	BB18	COI	45	BB80147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB18	TCTTATTCGTTGGAGACTTTATAACCCT	153.16121
BB80148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38	WGS84	BB80148	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80148_Cmae_Bot2011_COI	BB16	1	BB80148_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80148	Sample	BB80148	targeted	Absent	Sample	Cmae_Bot2011_COI	BB16	COI	45	BB80148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB16	ATGAACAGTCTATCCTCCTTTAG	153.16327
BB80148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38	WGS84	BB80148	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80148_Msal_Bot2012_COI	BB16	1	BB80148_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80148	Sample	BB80148	targeted	Absent	Sample	Msal_Bot2012_COI	BB16	COI	45	BB80148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB16	GYTAGTTCCRATGATGTTAGCTG	153.16327
BB80150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37228	WGS84	BB80150	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80150_Mgig_Bot2012_COI	BB11	1	BB80150_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80150	Sample	BB80150	targeted	Absent	Sample	Mgig_Bot2012_COI	BB11	COI	45	BB80150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB11	TCTTATTCGTTGGAGACTTTATAACCCT	153.16969
BB80151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35698	WGS84	BB80151	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80151_Cmae_Bot2011_COI	BB1	1	BB80151_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80151	Sample	BB80151	targeted	Absent	Sample	Cmae_Bot2011_COI	BB1	COI	45	BB80151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB1	ATGAACAGTCTATCCTCCTTTAG	153.18056
BB80151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35698	WGS84	BB80151	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80151_Msal_Bot2012_COI	BB1	1	BB80151_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80151	Sample	BB80151	targeted	Absent	Sample	Msal_Bot2012_COI	BB1	COI	45	BB80151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB1	GYTAGTTCCRATGATGTTAGCTG	153.18056
BB80153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44718	WGS84	BB80153	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80153_Aamu_Bax2006_COI	BB63	1	BB80153_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80153	Sample	BB80153	targeted	Absent	Sample	Aamu_Bax2006_COI	BB63	COI	45	BB80153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB63	GCACAACCGGGATCTTTACTTC	153.09844
BB80153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44718	WGS84	BB80153	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80153_Pcan_Bot2011_IGS	BB63	1	BB80153_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80153	Sample	BB80153	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB63	IGS	45	BB80153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB63	CGTAATCCTCAGTACTGGCTA	153.09844
BB80153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44718	WGS84	BB80153	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80153_Sspa_Oph2007_COI	BB63	1	BB80153_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80153	Sample	BB80153	targeted	Absent	Sample	Sspa_Oph2007_COI	BB63	COI	45	BB80153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB63	GAAGGCCTTCCGATGATC	153.09844
BB80153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44718	WGS84	BB80153	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80153_Upin_Bot2011_COI	BB63	1	BB80153_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80153	Sample	BB80153	targeted	Absent	Sample	Upin_Bot2011_COI	BB63	COI	45	BB80153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB63	CTTTAATTACAGCGTTTTTATTGTTGT	153.09844
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.933	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80154_Mgig_Bot2012_COI	MB90	1	BB80154_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Present	Sample	Mgig_Bot2012_COI	MB90	COI	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	TCTTATTCGTTGGAGACTTTATAACCCT	144.91468
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80155_Cmae_Bot2011_COI	MB85	1	BB80155_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Absent	Sample	Cmae_Bot2011_COI	MB85	COI	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	ATGAACAGTCTATCCTCCTTTAG	144.92112
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80155_Hsan_Wil2021_COI	MB85	1	BB80155_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Absent	Sample	Hsan_Wil2021_COI	MB85	COI	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	CCTGGGCCGGTATAGTAGGT	144.92112
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80155_Msal_Bot2012_COI	MB85	1	BB80155_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Absent	Sample	Msal_Bot2012_COI	MB85	COI	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	GYTAGTTCCRATGATGTTAGCTG	144.92112
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80157_Aamu_Bax2006_COI	MB65	1	BB80157_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Aamu_Bax2006_COI	MB65	COI	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	GCACAACCGGGATCTTTACTTC	144.922
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80157_Pcan_Bot2011_IGS	MB65	1	BB80157_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB65	IGS	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	CGTAATCCTCAGTACTGGCTA	144.922
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80157_Sspa_Oph2007_COI	MB65	1	BB80157_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Sspa_Oph2007_COI	MB65	COI	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	GAAGGCCTTCCGATGATC	144.922
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80157_Upin_Bot2011_COI	MB65	1	BB80157_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Upin_Bot2011_COI	MB65	COI	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	CTTTAATTACAGCGTTTTTATTGTTGT	144.922
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.722	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80158_Mgig_Bot2012_COI	MB60	1	BB80158_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Present	Sample	Mgig_Bot2012_COI	MB60	COI	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	TCTTATTCGTTGGAGACTTTATAACCCT	144.90884
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80159_Cmae_Bot2011_COI	MB105	1	BB80159_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Absent	Sample	Cmae_Bot2011_COI	MB105	COI	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	ATGAACAGTCTATCCTCCTTTAG	144.91163
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80159_Hsan_Wil2021_COI	MB105	1	BB80159_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Absent	Sample	Hsan_Wil2021_COI	MB105	COI	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	CCTGGGCCGGTATAGTAGGT	144.91163
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80159_Msal_Bot2012_COI	MB105	1	BB80159_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Absent	Sample	Msal_Bot2012_COI	MB105	COI	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	GYTAGTTCCRATGATGTTAGCTG	144.91163
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80160_Aamu_Bax2006_COI	MB100	1	BB80160_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Absent	Sample	Aamu_Bax2006_COI	MB100	COI	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	GCACAACCGGGATCTTTACTTC	144.91317
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80160_Pcan_Bot2011_IGS	MB100	1	BB80160_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB100	IGS	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	CGTAATCCTCAGTACTGGCTA	144.91317
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.21546555	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80160_Sspa_Oph2007_COI	MB100	1	BB80160_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Present	Sample	Sspa_Oph2007_COI	MB100	COI	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	GAAGGCCTTCCGATGATC	144.91317
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80160_Upin_Bot2011_COI	MB100	1	BB80160_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Absent	Sample	Upin_Bot2011_COI	MB100	COI	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	CTTTAATTACAGCGTTTTTATTGTTGT	144.91317
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.599	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80161_Mgig_Bot2012_COI	MB115	1	BB80161_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Present	Sample	Mgig_Bot2012_COI	MB115	COI	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	TCTTATTCGTTGGAGACTTTATAACCCT	144.90568
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80162_Cmae_Bot2011_COI	MB110	1	BB80162_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Absent	Sample	Cmae_Bot2011_COI	MB110	COI	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	ATGAACAGTCTATCCTCCTTTAG	144.90763
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80162_Hsan_Wil2021_COI	MB110	1	BB80162_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Absent	Sample	Hsan_Wil2021_COI	MB110	COI	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	CCTGGGCCGGTATAGTAGGT	144.90763
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80162_Msal_Bot2012_COI	MB110	1	BB80162_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Absent	Sample	Msal_Bot2012_COI	MB110	COI	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	GYTAGTTCCRATGATGTTAGCTG	144.90763
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.309	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80164_Aamu_Bax2006_COI	MB45	1	BB80164_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Present	Sample	Aamu_Bax2006_COI	MB45	COI	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	GCACAACCGGGATCTTTACTTC	144.91008
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80164_Pcan_Bot2011_IGS	MB45	1	BB80164_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB45	IGS	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	CGTAATCCTCAGTACTGGCTA	144.91008
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.62365723	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80164_Sspa_Oph2007_COI	MB45	1	BB80164_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Present	Sample	Sspa_Oph2007_COI	MB45	COI	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	GAAGGCCTTCCGATGATC	144.91008
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.041	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80164_Upin_Bot2011_COI	MB45	1	BB80164_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Present	Sample	Upin_Bot2011_COI	MB45	COI	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	CTTTAATTACAGCGTTTTTATTGTTGT	144.91008
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80165_Mgig_Bot2012_COI	MB40	1	BB80165_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Absent	Sample	Mgig_Bot2012_COI	MB40	COI	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	TCTTATTCGTTGGAGACTTTATAACCCT	144.9135
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80166_Cmae_Bot2011_COI	MB35	1	BB80166_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Absent	Sample	Cmae_Bot2011_COI	MB35	COI	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	ATGAACAGTCTATCCTCCTTTAG	144.91773
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80166_Hsan_Wil2021_COI	MB35	1	BB80166_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Absent	Sample	Hsan_Wil2021_COI	MB35	COI	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	CCTGGGCCGGTATAGTAGGT	144.91773
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80166_Msal_Bot2012_COI	MB35	1	BB80166_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Absent	Sample	Msal_Bot2012_COI	MB35	COI	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	GYTAGTTCCRATGATGTTAGCTG	144.91773
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.339	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80168_Aamu_Bax2006_COI	MB5	1	BB80168_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Present	Sample	Aamu_Bax2006_COI	MB5	COI	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	GCACAACCGGGATCTTTACTTC	144.9048
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80168_Pcan_Bot2011_IGS	MB5	1	BB80168_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB5	IGS	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	CGTAATCCTCAGTACTGGCTA	144.9048
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.34209824	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80168_Sspa_Oph2007_COI	MB5	1	BB80168_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Present	Sample	Sspa_Oph2007_COI	MB5	COI	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	GAAGGCCTTCCGATGATC	144.9048
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.081	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80168_Upin_Bot2011_COI	MB5	1	BB80168_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Present	Sample	Upin_Bot2011_COI	MB5	COI	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	CTTTAATTACAGCGTTTTTATTGTTGT	144.9048
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80169_Mgig_Bot2012_COI	MB15	1	BB80169_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Absent	Sample	Mgig_Bot2012_COI	MB15	COI	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	TCTTATTCGTTGGAGACTTTATAACCCT	144.90877
BB80171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84287	WGS84	BB80171	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.81	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80171_Aamu_Bax2006_COI	MB20	1	BB80171_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80171	Sample	BB80171	targeted	Present	Sample	Aamu_Bax2006_COI	MB20	COI	45	BB80171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB20	GCACAACCGGGATCTTTACTTC	144.92678
BB80171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84287	WGS84	BB80171	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80171_Pcan_Bot2011_IGS	MB20	1	BB80171_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80171	Sample	BB80171	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB20	IGS	45	BB80171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB20	CGTAATCCTCAGTACTGGCTA	144.92678
BB80171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84287	WGS84	BB80171	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80171_Sspa_Oph2007_COI	MB20	1	BB80171_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80171	Sample	BB80171	targeted	Absent	Sample	Sspa_Oph2007_COI	MB20	COI	45	BB80171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB20	GAAGGCCTTCCGATGATC	144.92678
BB80171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84287	WGS84	BB80171	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.53	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80171_Upin_Bot2011_COI	MB20	1	BB80171_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80171	Sample	BB80171	targeted	Present	Sample	Upin_Bot2011_COI	MB20	COI	45	BB80171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB20	CTTTAATTACAGCGTTTTTATTGTTGT	144.92678
BB80177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81974	WGS84	BB80177	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80177_Cmae_Bot2011_COI	MB108	1	BB80177_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80177	Sample	BB80177	targeted	Absent	Sample	Cmae_Bot2011_COI	MB108	COI	45	BB80177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB108	ATGAACAGTCTATCCTCCTTTAG	144.90959
BB80177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81974	WGS84	BB80177	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80177_Msal_Bot2012_COI	MB108	1	BB80177_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80177	Sample	BB80177	targeted	Absent	Sample	Msal_Bot2012_COI	MB108	COI	45	BB80177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB108	GYTAGTTCCRATGATGTTAGCTG	144.90959
BB80179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81378	WGS84	BB80179	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80179_Aamu_Bax2006_COI	MB102	1	BB80179_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80179	Sample	BB80179	targeted	Absent	Sample	Aamu_Bax2006_COI	MB102	COI	45	BB80179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB102	GCACAACCGGGATCTTTACTTC	144.91311
BB80179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81378	WGS84	BB80179	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80179_Pcan_Bot2011_IGS	MB102	1	BB80179_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80179	Sample	BB80179	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB102	IGS	45	BB80179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB102	CGTAATCCTCAGTACTGGCTA	144.91311
BB80179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81378	WGS84	BB80179	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.75001526	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80179_Sspa_Oph2007_COI	MB102	1	BB80179_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80179	Sample	BB80179	targeted	Present	Sample	Sspa_Oph2007_COI	MB102	COI	45	BB80179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB102	GAAGGCCTTCCGATGATC	144.91311
BB80179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81378	WGS84	BB80179	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	41.002	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80179_Upin_Bot2011_COI	MB102	1	BB80179_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80179	Sample	BB80179	targeted	Present	Sample	Upin_Bot2011_COI	MB102	COI	45	BB80179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB102	CTTTAATTACAGCGTTTTTATTGTTGT	144.91311
BB80180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81	WGS84	BB80180	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80180_Cmae_Bot2011_COI	MB98	1	BB80180_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80180	Sample	BB80180	targeted	Absent	Sample	Cmae_Bot2011_COI	MB98	COI	45	BB80180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB98	ATGAACAGTCTATCCTCCTTTAG	144.91304
BB80180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81	WGS84	BB80180	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80180_Msal_Bot2012_COI	MB98	1	BB80180_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80180	Sample	BB80180	targeted	Absent	Sample	Msal_Bot2012_COI	MB98	COI	45	BB80180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB98	GYTAGTTCCRATGATGTTAGCTG	144.91304
BB80182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81211	WGS84	BB80182	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80182_Aamu_Bax2006_COI	MB94	1	BB80182_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80182	Sample	BB80182	targeted	Absent	Sample	Aamu_Bax2006_COI	MB94	COI	45	BB80182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB94	GCACAACCGGGATCTTTACTTC	144.91485
BB80182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81211	WGS84	BB80182	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80182_Pcan_Bot2011_IGS	MB94	1	BB80182_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80182	Sample	BB80182	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB94	IGS	45	BB80182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB94	CGTAATCCTCAGTACTGGCTA	144.91485
BB80182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81211	WGS84	BB80182	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.24830246	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80182_Sspa_Oph2007_COI	MB94	1	BB80182_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80182	Sample	BB80182	targeted	Present	Sample	Sspa_Oph2007_COI	MB94	COI	45	BB80182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB94	GAAGGCCTTCCGATGATC	144.91485
BB80182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81211	WGS84	BB80182	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80182_Upin_Bot2011_COI	MB94	1	BB80182_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80182	Sample	BB80182	targeted	Absent	Sample	Upin_Bot2011_COI	MB94	COI	45	BB80182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB94	CTTTAATTACAGCGTTTTTATTGTTGT	144.91485
BB80183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81479	WGS84	BB80183	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.092	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80183_Mgig_Bot2012_COI	MB92	1	BB80183_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80183	Sample	BB80183	targeted	Present	Sample	Mgig_Bot2012_COI	MB92	COI	45	BB80183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB92	TCTTATTCGTTGGAGACTTTATAACCCT	144.91433
BB80184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81828	WGS84	BB80184	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80184_Cmae_Bot2011_COI	MB89	1	BB80184_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80184	Sample	BB80184	targeted	Absent	Sample	Cmae_Bot2011_COI	MB89	COI	45	BB80184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB89	ATGAACAGTCTATCCTCCTTTAG	144.91499
BB80184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81828	WGS84	BB80184	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80184_Msal_Bot2012_COI	MB89	1	BB80184_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80184	Sample	BB80184	targeted	Absent	Sample	Msal_Bot2012_COI	MB89	COI	45	BB80184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB89	GYTAGTTCCRATGATGTTAGCTG	144.91499
BB80186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81617	WGS84	BB80186	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80186_Aamu_Bax2006_COI	MB83	1	BB80186_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80186	Sample	BB80186	targeted	Absent	Sample	Aamu_Bax2006_COI	MB83	COI	45	BB80186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB83	GCACAACCGGGATCTTTACTTC	144.92378
BB80186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81617	WGS84	BB80186	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80186_Pcan_Bot2011_IGS	MB83	1	BB80186_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80186	Sample	BB80186	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB83	IGS	45	BB80186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB83	CGTAATCCTCAGTACTGGCTA	144.92378
BB80186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81617	WGS84	BB80186	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.94107437	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80186_Sspa_Oph2007_COI	MB83	1	BB80186_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80186	Sample	BB80186	targeted	Present	Sample	Sspa_Oph2007_COI	MB83	COI	45	BB80186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB83	GAAGGCCTTCCGATGATC	144.92378
BB80186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81617	WGS84	BB80186	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80186_Upin_Bot2011_COI	MB83	1	BB80186_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80186	Sample	BB80186	targeted	Absent	Sample	Upin_Bot2011_COI	MB83	COI	45	BB80186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB83	CTTTAATTACAGCGTTTTTATTGTTGT	144.92378
BB80187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81514	WGS84	BB80187	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.309	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80187_Mgig_Bot2012_COI	MB81	1	BB80187_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80187	Sample	BB80187	targeted	Present	Sample	Mgig_Bot2012_COI	MB81	COI	45	BB80187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB81	TCTTATTCGTTGGAGACTTTATAACCCT	144.92658
BB80188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81667	WGS84	BB80188	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80188_Cmae_Bot2011_COI	MB78	1	BB80188_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80188	Sample	BB80188	targeted	Absent	Sample	Cmae_Bot2011_COI	MB78	COI	45	BB80188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB78	ATGAACAGTCTATCCTCCTTTAG	144.92656
BB80188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81667	WGS84	BB80188	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80188_Msal_Bot2012_COI	MB78	1	BB80188_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80188	Sample	BB80188	targeted	Absent	Sample	Msal_Bot2012_COI	MB78	COI	45	BB80188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB78	GYTAGTTCCRATGATGTTAGCTG	144.92656
BB80190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81812	WGS84	BB80190	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80190_Mgig_Bot2012_COI	MB73	1	BB80190_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80190	Sample	BB80190	targeted	Absent	Sample	Mgig_Bot2012_COI	MB73	COI	45	BB80190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB73	TCTTATTCGTTGGAGACTTTATAACCCT	144.93004
BB80191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81807	WGS84	BB80191	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80191_Cmae_Bot2011_COI	MB72	1	BB80191_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80191	Sample	BB80191	targeted	Absent	Sample	Cmae_Bot2011_COI	MB72	COI	45	BB80191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB72	ATGAACAGTCTATCCTCCTTTAG	144.93189
BB80191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81807	WGS84	BB80191	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80191_Msal_Bot2012_COI	MB72	1	BB80191_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80191	Sample	BB80191	targeted	Absent	Sample	Msal_Bot2012_COI	MB72	COI	45	BB80191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB72	GYTAGTTCCRATGATGTTAGCTG	144.93189
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82058	WGS84	BB80193	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80193_Aamu_Bax2006_COI	MB69	1	BB80193_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Absent	Sample	Aamu_Bax2006_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	GCACAACCGGGATCTTTACTTC	144.93081
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82058	WGS84	BB80193	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80193_Pcan_Bot2011_IGS	MB69	1	BB80193_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB69	IGS	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	CGTAATCCTCAGTACTGGCTA	144.93081
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82058	WGS84	BB80193	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80193_Sspa_Oph2007_COI	MB69	1	BB80193_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Absent	Sample	Sspa_Oph2007_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	GAAGGCCTTCCGATGATC	144.93081
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82058	WGS84	BB80193	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80193_Upin_Bot2011_COI	MB69	1	BB80193_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Absent	Sample	Upin_Bot2011_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	CTTTAATTACAGCGTTTTTATTGTTGT	144.93081
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82058	WGS84	BB80193	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80193_Vgib_Wil2025_COI	MB69	1	BB80193_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Absent	Sample	Vgib_Wil2025_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	CCCCTCTTTACAAGTTCTTTAGGGT	144.93081
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82022	WGS84	BB80194	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.835	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80194_Asen_Wil2025_COI	MB67	1	BB80194_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Present	Sample	Asen_Wil2025_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	GTTGTTGTTACAACACATGCTCTTTTG	144.92791
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82022	WGS84	BB80194	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80194_Mgig_Bot2012_COI	MB67	1	BB80194_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Mgig_Bot2012_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	TCTTATTCGTTGGAGACTTTATAACCCT	144.92791
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81776	WGS84	BB80195	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80195_Cmae_Bot2011_COI	MB77	1	BB80195_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Absent	Sample	Cmae_Bot2011_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	ATGAACAGTCTATCCTCCTTTAG	144.92569
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81776	WGS84	BB80195	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80195_Msal_Bot2012_COI	MB77	1	BB80195_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Absent	Sample	Msal_Bot2012_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	GYTAGTTCCRATGATGTTAGCTG	144.92569
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82016	WGS84	BB80197	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.604	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80197_Aamu_Bax2006_COI	MB63	1	BB80197_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Present	Sample	Aamu_Bax2006_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	GCACAACCGGGATCTTTACTTC	144.91788
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82016	WGS84	BB80197	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80197_Pcan_Bot2011_IGS	MB63	1	BB80197_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB63	IGS	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	CGTAATCCTCAGTACTGGCTA	144.91788
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82016	WGS84	BB80197	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.65264893	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80197_Sspa_Oph2007_COI	MB63	1	BB80197_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Present	Sample	Sspa_Oph2007_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	GAAGGCCTTCCGATGATC	144.91788
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82016	WGS84	BB80197	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80197_Upin_Bot2011_COI	MB63	1	BB80197_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Absent	Sample	Upin_Bot2011_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	CTTTAATTACAGCGTTTTTATTGTTGT	144.91788
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82016	WGS84	BB80197	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	35.561	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80197_Vgib_Wil2025_COI	MB63	1	BB80197_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Present	Sample	Vgib_Wil2025_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	CCCCTCTTTACAAGTTCTTTAGGGT	144.91788
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82068	WGS84	BB80198	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.848	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80198_Asen_Wil2025_COI	MB62	1	BB80198_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Present	Sample	Asen_Wil2025_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	GTTGTTGTTACAACACATGCTCTTTTG	144.91443
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82068	WGS84	BB80198	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.509	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80198_Mgig_Bot2012_COI	MB62	1	BB80198_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Present	Sample	Mgig_Bot2012_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	TCTTATTCGTTGGAGACTTTATAACCCT	144.91443
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82144	WGS84	BB80199	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80199_Cmae_Bot2011_COI	MB61	1	BB80199_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Absent	Sample	Cmae_Bot2011_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	ATGAACAGTCTATCCTCCTTTAG	144.91152
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82144	WGS84	BB80199	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80199_Msal_Bot2012_COI	MB61	1	BB80199_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Absent	Sample	Msal_Bot2012_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	GYTAGTTCCRATGATGTTAGCTG	144.91152
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82853	WGS84	BB80201	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80201_Aamu_Bax2006_COI	MB56	1	BB80201_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Absent	Sample	Aamu_Bax2006_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	GCACAACCGGGATCTTTACTTC	144.90063
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82853	WGS84	BB80201	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80201_Pcan_Bot2011_IGS	MB56	1	BB80201_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB56	IGS	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	CGTAATCCTCAGTACTGGCTA	144.90063
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82853	WGS84	BB80201	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.20487213	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80201_Sspa_Oph2007_COI	MB56	1	BB80201_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Present	Sample	Sspa_Oph2007_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	GAAGGCCTTCCGATGATC	144.90063
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82853	WGS84	BB80201	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80201_Upin_Bot2011_COI	MB56	1	BB80201_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Absent	Sample	Upin_Bot2011_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	CTTTAATTACAGCGTTTTTATTGTTGT	144.90063
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82853	WGS84	BB80201	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80201_Vgib_Wil2025_COI	MB56	1	BB80201_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Absent	Sample	Vgib_Wil2025_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	CCCCTCTTTACAAGTTCTTTAGGGT	144.90063
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82694	WGS84	BB80202	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.008	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80202_Asen_Wil2025_COI	MB126	1	BB80202_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Present	Sample	Asen_Wil2025_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	GTTGTTGTTACAACACATGCTCTTTTG	144.89947
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82694	WGS84	BB80202	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.248	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80202_Mgig_Bot2012_COI	MB126	1	BB80202_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Present	Sample	Mgig_Bot2012_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	TCTTATTCGTTGGAGACTTTATAACCCT	144.89947
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.825	WGS84	BB80203	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80203_Cmae_Bot2011_COI	MB124	1	BB80203_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Absent	Sample	Cmae_Bot2011_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	ATGAACAGTCTATCCTCCTTTAG	144.90061
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.825	WGS84	BB80203	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80203_Msal_Bot2012_COI	MB124	1	BB80203_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Absent	Sample	Msal_Bot2012_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	GYTAGTTCCRATGATGTTAGCTG	144.90061
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82145	WGS84	BB80205	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80205_Aamu_Bax2006_COI	MB119	1	BB80205_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Absent	Sample	Aamu_Bax2006_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	GCACAACCGGGATCTTTACTTC	144.90501
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82145	WGS84	BB80205	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80205_Pcan_Bot2011_IGS	MB119	1	BB80205_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB119	IGS	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	CGTAATCCTCAGTACTGGCTA	144.90501
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82145	WGS84	BB80205	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.57886124	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80205_Sspa_Oph2007_COI	MB119	1	BB80205_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Present	Sample	Sspa_Oph2007_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	GAAGGCCTTCCGATGATC	144.90501
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82145	WGS84	BB80205	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80205_Upin_Bot2011_COI	MB119	1	BB80205_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Absent	Sample	Upin_Bot2011_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	CTTTAATTACAGCGTTTTTATTGTTGT	144.90501
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82145	WGS84	BB80205	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80205_Vgib_Wil2025_COI	MB119	1	BB80205_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Absent	Sample	Vgib_Wil2025_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	CCCCTCTTTACAAGTTCTTTAGGGT	144.90501
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81765	WGS84	BB80206	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	32.346	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80206_Asen_Wil2025_COI	MB116	1	BB80206_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Present	Sample	Asen_Wil2025_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	GTTGTTGTTACAACACATGCTCTTTTG	144.9054
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81765	WGS84	BB80206	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.535	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80206_Mgig_Bot2012_COI	MB116	1	BB80206_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Present	Sample	Mgig_Bot2012_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	TCTTATTCGTTGGAGACTTTATAACCCT	144.9054
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81725	WGS84	BB80207	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80207_Cmae_Bot2011_COI	MB113	1	BB80207_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Absent	Sample	Cmae_Bot2011_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	ATGAACAGTCTATCCTCCTTTAG	144.90634
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81725	WGS84	BB80207	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80207_Msal_Bot2012_COI	MB113	1	BB80207_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Absent	Sample	Msal_Bot2012_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	GYTAGTTCCRATGATGTTAGCTG	144.90634
BB80209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85495	WGS84	BB80209	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80209_Aamu_Bax2006_COI	MB54	1	BB80209_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80209	Sample	BB80209	targeted	Absent	Sample	Aamu_Bax2006_COI	MB54	COI	45	BB80209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB54	GCACAACCGGGATCTTTACTTC	144.90386
BB80209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85495	WGS84	BB80209	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80209_Pcan_Bot2011_IGS	MB54	1	BB80209_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80209	Sample	BB80209	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB54	IGS	45	BB80209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB54	CGTAATCCTCAGTACTGGCTA	144.90386
BB80209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85495	WGS84	BB80209	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.56788254	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80209_Sspa_Oph2007_COI	MB54	1	BB80209_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80209	Sample	BB80209	targeted	Present	Sample	Sspa_Oph2007_COI	MB54	COI	45	BB80209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB54	GAAGGCCTTCCGATGATC	144.90386
BB80209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85495	WGS84	BB80209	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.828	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80209_Upin_Bot2011_COI	MB54	1	BB80209_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80209	Sample	BB80209	targeted	Present	Sample	Upin_Bot2011_COI	MB54	COI	45	BB80209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB54	CTTTAATTACAGCGTTTTTATTGTTGT	144.90386
BB80210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85743	WGS84	BB80210	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80210_Cmae_Bot2011_COI	MB52	1	BB80210_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80210	Sample	BB80210	targeted	Absent	Sample	Cmae_Bot2011_COI	MB52	COI	45	BB80210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB52	ATGAACAGTCTATCCTCCTTTAG	144.90744
BB80210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85743	WGS84	BB80210	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80210_Msal_Bot2012_COI	MB52	1	BB80210_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80210	Sample	BB80210	targeted	Absent	Sample	Msal_Bot2012_COI	MB52	COI	45	BB80210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB52	GYTAGTTCCRATGATGTTAGCTG	144.90744
BB80212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86015	WGS84	BB80212	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.728	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80212_Aamu_Bax2006_COI	MB47	1	BB80212_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80212	Sample	BB80212	targeted	Present	Sample	Aamu_Bax2006_COI	MB47	COI	45	BB80212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB47	GCACAACCGGGATCTTTACTTC	144.90951
BB80212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86015	WGS84	BB80212	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80212_Pcan_Bot2011_IGS	MB47	1	BB80212_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80212	Sample	BB80212	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB47	IGS	45	BB80212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB47	CGTAATCCTCAGTACTGGCTA	144.90951
BB80212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86015	WGS84	BB80212	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80212_Sspa_Oph2007_COI	MB47	1	BB80212_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80212	Sample	BB80212	targeted	Absent	Sample	Sspa_Oph2007_COI	MB47	COI	45	BB80212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB47	GAAGGCCTTCCGATGATC	144.90951
BB80212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86015	WGS84	BB80212	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.262	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80212_Upin_Bot2011_COI	MB47	1	BB80212_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80212	Sample	BB80212	targeted	Present	Sample	Upin_Bot2011_COI	MB47	COI	45	BB80212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB47	CTTTAATTACAGCGTTTTTATTGTTGT	144.90951
BB80213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86038	WGS84	BB80213	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80213_Mgig_Bot2012_COI	MB46	1	BB80213_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80213	Sample	BB80213	targeted	Absent	Sample	Mgig_Bot2012_COI	MB46	COI	45	BB80213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB46	TCTTATTCGTTGGAGACTTTATAACCCT	144.91026
BB80214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86081	WGS84	BB80214	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80214_Cmae_Bot2011_COI	MB43	1	BB80214_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80214	Sample	BB80214	targeted	Absent	Sample	Cmae_Bot2011_COI	MB43	COI	45	BB80214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB43	ATGAACAGTCTATCCTCCTTTAG	144.91408
BB80214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86081	WGS84	BB80214	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80214_Msal_Bot2012_COI	MB43	1	BB80214_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80214	Sample	BB80214	targeted	Absent	Sample	Msal_Bot2012_COI	MB43	COI	45	BB80214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB43	GYTAGTTCCRATGATGTTAGCTG	144.91408
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.371	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80216_Aamu_Bax2006_COI	MB38	1	BB80216_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Present	Sample	Aamu_Bax2006_COI	MB38	COI	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	GCACAACCGGGATCTTTACTTC	144.91498
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80216_Pcan_Bot2011_IGS	MB38	1	BB80216_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB38	IGS	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	CGTAATCCTCAGTACTGGCTA	144.91498
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.94107819	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80216_Sspa_Oph2007_COI	MB38	1	BB80216_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Present	Sample	Sspa_Oph2007_COI	MB38	COI	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	GAAGGCCTTCCGATGATC	144.91498
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.085	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80216_Upin_Bot2011_COI	MB38	1	BB80216_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Present	Sample	Upin_Bot2011_COI	MB38	COI	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	CTTTAATTACAGCGTTTTTATTGTTGT	144.91498
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80217_Mgig_Bot2012_COI	MB33	1	BB80217_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Absent	Sample	Mgig_Bot2012_COI	MB33	COI	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	TCTTATTCGTTGGAGACTTTATAACCCT	144.91683
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80218_Cmae_Bot2011_COI	MB32	1	BB80218_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Absent	Sample	Cmae_Bot2011_COI	MB32	COI	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	ATGAACAGTCTATCCTCCTTTAG	144.91483
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80218_Hsan_Wil2021_COI	MB32	1	BB80218_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Absent	Sample	Hsan_Wil2021_COI	MB32	COI	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	CCTGGGCCGGTATAGTAGGT	144.91483
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80218_Msal_Bot2012_COI	MB32	1	BB80218_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Absent	Sample	Msal_Bot2012_COI	MB32	COI	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	GYTAGTTCCRATGATGTTAGCTG	144.91483
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80220_Mgig_Bot2012_COI	MB9	1	BB80220_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Absent	Sample	Mgig_Bot2012_COI	MB9	COI	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	TCTTATTCGTTGGAGACTTTATAACCCT	144.90576
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80221_Cmae_Bot2011_COI	MB7	1	BB80221_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Absent	Sample	Cmae_Bot2011_COI	MB7	COI	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	ATGAACAGTCTATCCTCCTTTAG	144.90603
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80221_Hsan_Wil2021_COI	MB7	1	BB80221_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Absent	Sample	Hsan_Wil2021_COI	MB7	COI	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	CCTGGGCCGGTATAGTAGGT	144.90603
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80221_Msal_Bot2012_COI	MB7	1	BB80221_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Absent	Sample	Msal_Bot2012_COI	MB7	COI	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	GYTAGTTCCRATGATGTTAGCTG	144.90603
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.119	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80223_Aamu_Bax2006_COI	MB13	1	BB80223_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Present	Sample	Aamu_Bax2006_COI	MB13	COI	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	GCACAACCGGGATCTTTACTTC	144.91033
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80223_Pcan_Bot2011_IGS	MB13	1	BB80223_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB13	IGS	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	CGTAATCCTCAGTACTGGCTA	144.91033
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.20193863	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80223_Sspa_Oph2007_COI	MB13	1	BB80223_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Present	Sample	Sspa_Oph2007_COI	MB13	COI	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	GAAGGCCTTCCGATGATC	144.91033
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.96	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80223_Upin_Bot2011_COI	MB13	1	BB80223_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Present	Sample	Upin_Bot2011_COI	MB13	COI	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	CTTTAATTACAGCGTTTTTATTGTTGT	144.91033
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80224_Mgig_Bot2012_COI	MB12	1	BB80224_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Absent	Sample	Mgig_Bot2012_COI	MB12	COI	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	TCTTATTCGTTGGAGACTTTATAACCCT	144.90642
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80225_Cmae_Bot2011_COI	MB11	1	BB80225_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Absent	Sample	Cmae_Bot2011_COI	MB11	COI	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	ATGAACAGTCTATCCTCCTTTAG	144.90635
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80225_Hsan_Wil2021_COI	MB11	1	BB80225_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Absent	Sample	Hsan_Wil2021_COI	MB11	COI	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	CCTGGGCCGGTATAGTAGGT	144.90635
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80225_Msal_Bot2012_COI	MB11	1	BB80225_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Absent	Sample	Msal_Bot2012_COI	MB11	COI	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	GYTAGTTCCRATGATGTTAGCTG	144.90635
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.016	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80227_Aamu_Bax2006_COI	MB26	1	BB80227_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Present	Sample	Aamu_Bax2006_COI	MB26	COI	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	GCACAACCGGGATCTTTACTTC	144.92895
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80227_Pcan_Bot2011_IGS	MB26	1	BB80227_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB26	IGS	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	CGTAATCCTCAGTACTGGCTA	144.92895
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.22904968	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80227_Sspa_Oph2007_COI	MB26	1	BB80227_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Present	Sample	Sspa_Oph2007_COI	MB26	COI	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	GAAGGCCTTCCGATGATC	144.92895
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.091	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80227_Upin_Bot2011_COI	MB26	1	BB80227_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Present	Sample	Upin_Bot2011_COI	MB26	COI	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	CTTTAATTACAGCGTTTTTATTGTTGT	144.92895
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80228_Mgig_Bot2012_COI	MB23	1	BB80228_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Absent	Sample	Mgig_Bot2012_COI	MB23	COI	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	TCTTATTCGTTGGAGACTTTATAACCCT	144.92963
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80229_Cmae_Bot2011_COI	MB22	1	BB80229_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Absent	Sample	Cmae_Bot2011_COI	MB22	COI	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	ATGAACAGTCTATCCTCCTTTAG	144.92823
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80229_Hsan_Wil2021_COI	MB22	1	BB80229_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Absent	Sample	Hsan_Wil2021_COI	MB22	COI	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	CCTGGGCCGGTATAGTAGGT	144.92823
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80229_Msal_Bot2012_COI	MB22	1	BB80229_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Absent	Sample	Msal_Bot2012_COI	MB22	COI	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	GYTAGTTCCRATGATGTTAGCTG	144.92823
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.39	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80230_Aamu_Bax2006_COI	MB18	1	BB80230_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Present	Sample	Aamu_Bax2006_COI	MB18	COI	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	GCACAACCGGGATCTTTACTTC	144.92605
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80230_Pcan_Bot2011_IGS	MB18	1	BB80230_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB18	IGS	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	CGTAATCCTCAGTACTGGCTA	144.92605
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.16910553	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80230_Sspa_Oph2007_COI	MB18	1	BB80230_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Present	Sample	Sspa_Oph2007_COI	MB18	COI	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	GAAGGCCTTCCGATGATC	144.92605
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.632	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80230_Upin_Bot2011_COI	MB18	1	BB80230_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Present	Sample	Upin_Bot2011_COI	MB18	COI	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	CTTTAATTACAGCGTTTTTATTGTTGT	144.92605
BB80231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84499	WGS84	BB80231	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80231_Mgig_Bot2012_COI	HB20	1	BB80231_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80231	Sample	BB80231	targeted	Absent	Sample	Mgig_Bot2012_COI	HB20	COI	45	BB80231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB20	TCTTATTCGTTGGAGACTTTATAACCCT	147.32948
BB80232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88741	WGS84	BB80232	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.144	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80232_Cmae_Bot2011_COI	HB10	1	BB80232_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80232	Sample	BB80232	targeted	Present	Sample	Cmae_Bot2011_COI	HB10	COI	45	BB80232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB10	ATGAACAGTCTATCCTCCTTTAG	147.34018
BB80232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88741	WGS84	BB80232	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80232_Msal_Bot2012_COI	HB10	1	BB80232_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80232	Sample	BB80232	targeted	Absent	Sample	Msal_Bot2012_COI	HB10	COI	45	BB80232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB10	GYTAGTTCCRATGATGTTAGCTG	147.34018
BB80239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88538	WGS84	BB80239	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80239_Mgig_Bot2012_COI	HB9	1	BB80239_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80239	Sample	BB80239	targeted	Absent	Sample	Mgig_Bot2012_COI	HB9	COI	45	BB80239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB9	TCTTATTCGTTGGAGACTTTATAACCCT	147.33553
BB80241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88368	WGS84	BB80241	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.295	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80241_Aamu_Bax2006_COI	HB7	1	BB80241_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80241	Sample	BB80241	targeted	Present	Sample	Aamu_Bax2006_COI	HB7	COI	45	BB80241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB7	GCACAACCGGGATCTTTACTTC	147.33443
BB80241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88368	WGS84	BB80241	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80241_Pcan_Bot2011_IGS	HB7	1	BB80241_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80241	Sample	BB80241	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB7	IGS	45	BB80241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB7	CGTAATCCTCAGTACTGGCTA	147.33443
BB80241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88368	WGS84	BB80241	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80241_Sspa_Oph2007_COI	HB7	1	BB80241_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80241	Sample	BB80241	targeted	Absent	Sample	Sspa_Oph2007_COI	HB7	COI	45	BB80241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB7	GAAGGCCTTCCGATGATC	147.33443
BB80241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88368	WGS84	BB80241	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80241_Upin_Bot2011_COI	HB7	1	BB80241_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80241	Sample	BB80241	targeted	Absent	Sample	Upin_Bot2011_COI	HB7	COI	45	BB80241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB7	CTTTAATTACAGCGTTTTTATTGTTGT	147.33443
BB80242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88453	WGS84	BB80242	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80242_Mgig_Bot2012_COI	HB6	1	BB80242_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80242	Sample	BB80242	targeted	Absent	Sample	Mgig_Bot2012_COI	HB6	COI	45	BB80242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB6	TCTTATTCGTTGGAGACTTTATAACCCT	147.33358
BB80243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88098	WGS84	BB80243	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80243_Cmae_Bot2011_COI	HB13	1	BB80243_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80243	Sample	BB80243	targeted	Absent	Sample	Cmae_Bot2011_COI	HB13	COI	45	BB80243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB13	ATGAACAGTCTATCCTCCTTTAG	147.34197
BB80243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88098	WGS84	BB80243	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80243_Msal_Bot2012_COI	HB13	1	BB80243_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80243	Sample	BB80243	targeted	Absent	Sample	Msal_Bot2012_COI	HB13	COI	45	BB80243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB13	GYTAGTTCCRATGATGTTAGCTG	147.34197
BB80245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84503	WGS84	BB80245	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.469	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80245_Aamu_Bax2006_COI	HB21	1	BB80245_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80245	Sample	BB80245	targeted	Present	Sample	Aamu_Bax2006_COI	HB21	COI	45	BB80245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB21	GCACAACCGGGATCTTTACTTC	147.32826
BB80245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84503	WGS84	BB80245	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80245_Pcan_Bot2011_IGS	HB21	1	BB80245_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80245	Sample	BB80245	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB21	IGS	45	BB80245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB21	CGTAATCCTCAGTACTGGCTA	147.32826
BB80245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84503	WGS84	BB80245	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80245_Sspa_Oph2007_COI	HB21	1	BB80245_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80245	Sample	BB80245	targeted	Absent	Sample	Sspa_Oph2007_COI	HB21	COI	45	BB80245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB21	GAAGGCCTTCCGATGATC	147.32826
BB80245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84503	WGS84	BB80245	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80245_Upin_Bot2011_COI	HB21	1	BB80245_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80245	Sample	BB80245	targeted	Absent	Sample	Upin_Bot2011_COI	HB21	COI	45	BB80245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB21	CTTTAATTACAGCGTTTTTATTGTTGT	147.32826
BB80246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84502	WGS84	BB80246	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80246_Mgig_Bot2012_COI	HB18	1	BB80246_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80246	Sample	BB80246	targeted	Absent	Sample	Mgig_Bot2012_COI	HB18	COI	45	BB80246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB18	TCTTATTCGTTGGAGACTTTATAACCCT	147.33073
BB80247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84651	WGS84	BB80247	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.86	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80247_Cmae_Bot2011_COI	HB16	1	BB80247_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80247	Sample	BB80247	targeted	Present	Sample	Cmae_Bot2011_COI	HB16	COI	45	BB80247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB16	ATGAACAGTCTATCCTCCTTTAG	147.32893
BB80247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84651	WGS84	BB80247	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80247_Msal_Bot2012_COI	HB16	1	BB80247_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80247	Sample	BB80247	targeted	Absent	Sample	Msal_Bot2012_COI	HB16	COI	45	BB80247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB16	GYTAGTTCCRATGATGTTAGCTG	147.32893
BB80249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.83215	WGS84	BB80249	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.596	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80249_Aamu_Bax2006_COI	HB24	1	BB80249_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80249	Sample	BB80249	targeted	Present	Sample	Aamu_Bax2006_COI	HB24	COI	45	BB80249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB24	GCACAACCGGGATCTTTACTTC	147.32207
BB80249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.83215	WGS84	BB80249	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80249_Pcan_Bot2011_IGS	HB24	1	BB80249_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80249	Sample	BB80249	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB24	IGS	45	BB80249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB24	CGTAATCCTCAGTACTGGCTA	147.32207
BB80249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.83215	WGS84	BB80249	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80249_Sspa_Oph2007_COI	HB24	1	BB80249_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80249	Sample	BB80249	targeted	Absent	Sample	Sspa_Oph2007_COI	HB24	COI	45	BB80249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB24	GAAGGCCTTCCGATGATC	147.32207
BB80249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.83215	WGS84	BB80249	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80249_Upin_Bot2011_COI	HB24	1	BB80249_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80249	Sample	BB80249	targeted	Absent	Sample	Upin_Bot2011_COI	HB24	COI	45	BB80249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB24	CTTTAATTACAGCGTTTTTATTGTTGT	147.32207
BB80250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.82857	WGS84	BB80250	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.37	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80250_Cmae_Bot2011_COI	HB22	1	BB80250_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80250	Sample	BB80250	targeted	Present	Sample	Cmae_Bot2011_COI	HB22	COI	45	BB80250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB22	ATGAACAGTCTATCCTCCTTTAG	147.31482
BB80250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.82857	WGS84	BB80250	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80250_Msal_Bot2012_COI	HB22	1	BB80250_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80250	Sample	BB80250	targeted	Absent	Sample	Msal_Bot2012_COI	HB22	COI	45	BB80250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB22	GYTAGTTCCRATGATGTTAGCTG	147.31482
BB80252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79283	WGS84	BB80252	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80252_Aamu_Bax2006_COI	GB95	1	BB80252_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80252	Sample	BB80252	targeted	Absent	Sample	Aamu_Bax2006_COI	GB95	COI	45	BB80252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB95	GCACAACCGGGATCTTTACTTC	151.21146
BB80252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79283	WGS84	BB80252	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80252_Pcan_Bot2011_IGS	GB95	1	BB80252_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80252	Sample	BB80252	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB95	IGS	45	BB80252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB95	CGTAATCCTCAGTACTGGCTA	151.21146
BB80252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79283	WGS84	BB80252	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80252_Sspa_Oph2007_COI	GB95	1	BB80252_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80252	Sample	BB80252	targeted	Absent	Sample	Sspa_Oph2007_COI	GB95	COI	45	BB80252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB95	GAAGGCCTTCCGATGATC	151.21146
BB80252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79283	WGS84	BB80252	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80252_Upin_Bot2011_COI	GB95	1	BB80252_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80252	Sample	BB80252	targeted	Absent	Sample	Upin_Bot2011_COI	GB95	COI	45	BB80252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB95	CTTTAATTACAGCGTTTTTATTGTTGT	151.21146
BB80253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79251	WGS84	BB80253	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80253_Mgig_Bot2012_COI	GB60	1	BB80253_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80253	Sample	BB80253	targeted	Absent	Sample	Mgig_Bot2012_COI	GB60	COI	45	BB80253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB60	TCTTATTCGTTGGAGACTTTATAACCCT	151.18214
BB80254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78354	WGS84	BB80254	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80254_Cmae_Bot2011_COI	GB90	1	BB80254_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80254	Sample	BB80254	targeted	Absent	Sample	Cmae_Bot2011_COI	GB90	COI	45	BB80254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB90	ATGAACAGTCTATCCTCCTTTAG	151.20098
BB80254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78354	WGS84	BB80254	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80254_Msal_Bot2012_COI	GB90	1	BB80254_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80254	Sample	BB80254	targeted	Absent	Sample	Msal_Bot2012_COI	GB90	COI	45	BB80254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB90	GYTAGTTCCRATGATGTTAGCTG	151.20098
BB80256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81467	WGS84	BB80256	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80256_Aamu_Bax2006_COI	GB80	1	BB80256_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80256	Sample	BB80256	targeted	Absent	Sample	Aamu_Bax2006_COI	GB80	COI	45	BB80256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB80	GCACAACCGGGATCTTTACTTC	151.23642
BB80256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81467	WGS84	BB80256	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80256_Pcan_Bot2011_IGS	GB80	1	BB80256_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80256	Sample	BB80256	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB80	IGS	45	BB80256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB80	CGTAATCCTCAGTACTGGCTA	151.23642
BB80256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81467	WGS84	BB80256	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80256_Sspa_Oph2007_COI	GB80	1	BB80256_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80256	Sample	BB80256	targeted	Absent	Sample	Sspa_Oph2007_COI	GB80	COI	45	BB80256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB80	GAAGGCCTTCCGATGATC	151.23642
BB80256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81467	WGS84	BB80256	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80256_Upin_Bot2011_COI	GB80	1	BB80256_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80256	Sample	BB80256	targeted	Absent	Sample	Upin_Bot2011_COI	GB80	COI	45	BB80256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB80	CTTTAATTACAGCGTTTTTATTGTTGT	151.23642
BB80257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82328	WGS84	BB80257	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80257_Mgig_Bot2012_COI	GB70	1	BB80257_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80257	Sample	BB80257	targeted	Absent	Sample	Mgig_Bot2012_COI	GB70	COI	45	BB80257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB70	TCTTATTCGTTGGAGACTTTATAACCCT	151.24379
BB80258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78741	WGS84	BB80258	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80258_Cmae_Bot2011_COI	GB50	1	BB80258_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80258	Sample	BB80258	targeted	Absent	Sample	Cmae_Bot2011_COI	GB50	COI	45	BB80258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB50	ATGAACAGTCTATCCTCCTTTAG	151.1737
BB80258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78741	WGS84	BB80258	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80258_Msal_Bot2012_COI	GB50	1	BB80258_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80258	Sample	BB80258	targeted	Absent	Sample	Msal_Bot2012_COI	GB50	COI	45	BB80258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB50	GYTAGTTCCRATGATGTTAGCTG	151.1737
BB80260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80815	WGS84	BB80260	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80260_Mgig_Bot2012_COI	GB40	1	BB80260_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80260	Sample	BB80260	targeted	Absent	Sample	Mgig_Bot2012_COI	GB40	COI	45	BB80260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB40	TCTTATTCGTTGGAGACTTTATAACCCT	151.21644
BB80261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8056	WGS84	BB80261	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80261_Cmae_Bot2011_COI	GB35	1	BB80261_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80261	Sample	BB80261	targeted	Absent	Sample	Cmae_Bot2011_COI	GB35	COI	45	BB80261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB35	ATGAACAGTCTATCCTCCTTTAG	151.21581
BB80261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8056	WGS84	BB80261	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80261_Msal_Bot2012_COI	GB35	1	BB80261_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80261	Sample	BB80261	targeted	Absent	Sample	Msal_Bot2012_COI	GB35	COI	45	BB80261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB35	GYTAGTTCCRATGATGTTAGCTG	151.21581
BB80263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8365	WGS84	BB80263	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80263_Aamu_Bax2006_COI	GB15	1	BB80263_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80263	Sample	BB80263	targeted	Absent	Sample	Aamu_Bax2006_COI	GB15	COI	45	BB80263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB15	GCACAACCGGGATCTTTACTTC	151.2728
BB80263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8365	WGS84	BB80263	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80263_Pcan_Bot2011_IGS	GB15	1	BB80263_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80263	Sample	BB80263	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB15	IGS	45	BB80263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB15	CGTAATCCTCAGTACTGGCTA	151.2728
BB80263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8365	WGS84	BB80263	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80263_Sspa_Oph2007_COI	GB15	1	BB80263_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80263	Sample	BB80263	targeted	Absent	Sample	Sspa_Oph2007_COI	GB15	COI	45	BB80263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB15	GAAGGCCTTCCGATGATC	151.2728
BB80263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8365	WGS84	BB80263	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80263_Upin_Bot2011_COI	GB15	1	BB80263_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80263	Sample	BB80263	targeted	Absent	Sample	Upin_Bot2011_COI	GB15	COI	45	BB80263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB15	CTTTAATTACAGCGTTTTTATTGTTGT	151.2728
BB80264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85116	WGS84	BB80264	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80264_Mgig_Bot2012_COI	GB10	1	BB80264_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80264	Sample	BB80264	targeted	Absent	Sample	Mgig_Bot2012_COI	GB10	COI	45	BB80264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB10	TCTTATTCGTTGGAGACTTTATAACCCT	151.31425
BB80265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.853	WGS84	BB80265	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80265_Cmae_Bot2011_COI	GB5	1	BB80265_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80265	Sample	BB80265	targeted	Absent	Sample	Cmae_Bot2011_COI	GB5	COI	45	BB80265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB5	ATGAACAGTCTATCCTCCTTTAG	151.32026
BB80265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.853	WGS84	BB80265	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80265_Msal_Bot2012_COI	GB5	1	BB80265_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80265	Sample	BB80265	targeted	Absent	Sample	Msal_Bot2012_COI	GB5	COI	45	BB80265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB5	GYTAGTTCCRATGATGTTAGCTG	151.32026
BB80271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80759	WGS84	BB80271	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80271_Mgig_Bot2012_COI	GB43	1	BB80271_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80271	Sample	BB80271	targeted	Absent	Sample	Mgig_Bot2012_COI	GB43	COI	45	BB80271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB43	TCTTATTCGTTGGAGACTTTATAACCCT	151.22026
BB80272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80744	WGS84	BB80272	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80272_Cmae_Bot2011_COI	GB42	1	BB80272_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80272	Sample	BB80272	targeted	Absent	Sample	Cmae_Bot2011_COI	GB42	COI	45	BB80272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB42	ATGAACAGTCTATCCTCCTTTAG	151.21814
BB80272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80744	WGS84	BB80272	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80272_Msal_Bot2012_COI	GB42	1	BB80272_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80272	Sample	BB80272	targeted	Absent	Sample	Msal_Bot2012_COI	GB42	COI	45	BB80272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB42	GYTAGTTCCRATGATGTTAGCTG	151.21814
BB80274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80665	WGS84	BB80274	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80274_Aamu_Bax2006_COI	GB37	1	BB80274_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80274	Sample	BB80274	targeted	Absent	Sample	Aamu_Bax2006_COI	GB37	COI	45	BB80274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB37	GCACAACCGGGATCTTTACTTC	151.2207
BB80274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80665	WGS84	BB80274	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80274_Pcan_Bot2011_IGS	GB37	1	BB80274_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80274	Sample	BB80274	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB37	IGS	45	BB80274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB37	CGTAATCCTCAGTACTGGCTA	151.2207
BB80274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80665	WGS84	BB80274	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80274_Sspa_Oph2007_COI	GB37	1	BB80274_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80274	Sample	BB80274	targeted	Absent	Sample	Sspa_Oph2007_COI	GB37	COI	45	BB80274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB37	GAAGGCCTTCCGATGATC	151.2207
BB80274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80665	WGS84	BB80274	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80274_Upin_Bot2011_COI	GB37	1	BB80274_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80274	Sample	BB80274	targeted	Absent	Sample	Upin_Bot2011_COI	GB37	COI	45	BB80274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB37	CTTTAATTACAGCGTTTTTATTGTTGT	151.2207
BB80275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79118	WGS84	BB80275	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80275_Mgig_Bot2012_COI	GB63	1	BB80275_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80275	Sample	BB80275	targeted	Absent	Sample	Mgig_Bot2012_COI	GB63	COI	45	BB80275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB63	TCTTATTCGTTGGAGACTTTATAACCCT	151.17737
BB80276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79094	WGS84	BB80276	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80276_Cmae_Bot2011_COI	GB58	1	BB80276_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80276	Sample	BB80276	targeted	Absent	Sample	Cmae_Bot2011_COI	GB58	COI	45	BB80276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB58	ATGAACAGTCTATCCTCCTTTAG	151.1826
BB80276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79094	WGS84	BB80276	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80276_Msal_Bot2012_COI	GB58	1	BB80276_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80276	Sample	BB80276	targeted	Absent	Sample	Msal_Bot2012_COI	GB58	COI	45	BB80276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB58	GYTAGTTCCRATGATGTTAGCTG	151.1826
BB80278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78795	WGS84	BB80278	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80278_Aamu_Bax2006_COI	GB56	1	BB80278_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80278	Sample	BB80278	targeted	Absent	Sample	Aamu_Bax2006_COI	GB56	COI	45	BB80278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB56	GCACAACCGGGATCTTTACTTC	151.17782
BB80278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78795	WGS84	BB80278	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80278_Pcan_Bot2011_IGS	GB56	1	BB80278_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80278	Sample	BB80278	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB56	IGS	45	BB80278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB56	CGTAATCCTCAGTACTGGCTA	151.17782
BB80278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78795	WGS84	BB80278	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80278_Sspa_Oph2007_COI	GB56	1	BB80278_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80278	Sample	BB80278	targeted	Absent	Sample	Sspa_Oph2007_COI	GB56	COI	45	BB80278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB56	GAAGGCCTTCCGATGATC	151.17782
BB80278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78795	WGS84	BB80278	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80278_Upin_Bot2011_COI	GB56	1	BB80278_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80278	Sample	BB80278	targeted	Absent	Sample	Upin_Bot2011_COI	GB56	COI	45	BB80278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB56	CTTTAATTACAGCGTTTTTATTGTTGT	151.17782
BB80279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78885	WGS84	BB80279	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80279_Mgig_Bot2012_COI	GB54	1	BB80279_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80279	Sample	BB80279	targeted	Absent	Sample	Mgig_Bot2012_COI	GB54	COI	45	BB80279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB54	TCTTATTCGTTGGAGACTTTATAACCCT	151.17702
BB80281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78569	WGS84	BB80281	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80281_Aamu_Bax2006_COI	GB48	1	BB80281_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80281	Sample	BB80281	targeted	Absent	Sample	Aamu_Bax2006_COI	GB48	COI	45	BB80281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB48	GCACAACCGGGATCTTTACTTC	151.17473
BB80281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78569	WGS84	BB80281	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80281_Pcan_Bot2011_IGS	GB48	1	BB80281_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80281	Sample	BB80281	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB48	IGS	45	BB80281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB48	CGTAATCCTCAGTACTGGCTA	151.17473
BB80281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78569	WGS84	BB80281	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80281_Sspa_Oph2007_COI	GB48	1	BB80281_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80281	Sample	BB80281	targeted	Absent	Sample	Sspa_Oph2007_COI	GB48	COI	45	BB80281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB48	GAAGGCCTTCCGATGATC	151.17473
BB80281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78569	WGS84	BB80281	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80281_Upin_Bot2011_COI	GB48	1	BB80281_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80281	Sample	BB80281	targeted	Absent	Sample	Upin_Bot2011_COI	GB48	COI	45	BB80281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB48	CTTTAATTACAGCGTTTTTATTGTTGT	151.17473
BB80282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80282	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80282_Mgig_Bot2012_COI	GB46	1	BB80282_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80282	Sample	BB80282	targeted	Absent	Sample	Mgig_Bot2012_COI	GB46	COI	45	BB80282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB46	TCTTATTCGTTGGAGACTTTATAACCCT	151.17273
BB80283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80283	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80283_Cmae_Bot2011_COI	GB92	1	BB80283_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80283	Sample	BB80283	targeted	Absent	Sample	Cmae_Bot2011_COI	GB92	COI	45	BB80283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB92	ATGAACAGTCTATCCTCCTTTAG	151.20462
BB80283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80283	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80283_Msal_Bot2012_COI	GB92	1	BB80283_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80283	Sample	BB80283	targeted	Absent	Sample	Msal_Bot2012_COI	GB92	COI	45	BB80283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB92	GYTAGTTCCRATGATGTTAGCTG	151.20462
BB80285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77979	WGS84	BB80285	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80285_Aamu_Bax2006_COI	GB87	1	BB80285_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80285	Sample	BB80285	targeted	Absent	Sample	Aamu_Bax2006_COI	GB87	COI	45	BB80285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB87	GCACAACCGGGATCTTTACTTC	151.19725
BB80285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77979	WGS84	BB80285	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80285_Pcan_Bot2011_IGS	GB87	1	BB80285_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80285	Sample	BB80285	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB87	IGS	45	BB80285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB87	CGTAATCCTCAGTACTGGCTA	151.19725
BB80285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77979	WGS84	BB80285	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80285_Sspa_Oph2007_COI	GB87	1	BB80285_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80285	Sample	BB80285	targeted	Absent	Sample	Sspa_Oph2007_COI	GB87	COI	45	BB80285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB87	GAAGGCCTTCCGATGATC	151.19725
BB80285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77979	WGS84	BB80285	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80285_Upin_Bot2011_COI	GB87	1	BB80285_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80285	Sample	BB80285	targeted	Absent	Sample	Upin_Bot2011_COI	GB87	COI	45	BB80285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB87	CTTTAATTACAGCGTTTTTATTGTTGT	151.19725
BB80286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81603	WGS84	BB80286	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80286_Mgig_Bot2012_COI	GB82	1	BB80286_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80286	Sample	BB80286	targeted	Absent	Sample	Mgig_Bot2012_COI	GB82	COI	45	BB80286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB82	TCTTATTCGTTGGAGACTTTATAACCCT	151.2366
BB80287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81718	WGS84	BB80287	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80287_Cmae_Bot2011_COI	GB84	1	BB80287_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80287	Sample	BB80287	targeted	Absent	Sample	Cmae_Bot2011_COI	GB84	COI	45	BB80287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB84	ATGAACAGTCTATCCTCCTTTAG	151.23821
BB80287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81718	WGS84	BB80287	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80287_Msal_Bot2012_COI	GB84	1	BB80287_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80287	Sample	BB80287	targeted	Absent	Sample	Msal_Bot2012_COI	GB84	COI	45	BB80287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB84	GYTAGTTCCRATGATGTTAGCTG	151.23821
BB80289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81519	WGS84	BB80289	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80289_Aamu_Bax2006_COI	GB79	1	BB80289_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80289	Sample	BB80289	targeted	Absent	Sample	Aamu_Bax2006_COI	GB79	COI	45	BB80289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB79	GCACAACCGGGATCTTTACTTC	151.23887
BB80289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81519	WGS84	BB80289	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80289_Pcan_Bot2011_IGS	GB79	1	BB80289_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80289	Sample	BB80289	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB79	IGS	45	BB80289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB79	CGTAATCCTCAGTACTGGCTA	151.23887
BB80289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81519	WGS84	BB80289	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80289_Sspa_Oph2007_COI	GB79	1	BB80289_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80289	Sample	BB80289	targeted	Absent	Sample	Sspa_Oph2007_COI	GB79	COI	45	BB80289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB79	GAAGGCCTTCCGATGATC	151.23887
BB80289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81519	WGS84	BB80289	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80289_Upin_Bot2011_COI	GB79	1	BB80289_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80289	Sample	BB80289	targeted	Absent	Sample	Upin_Bot2011_COI	GB79	COI	45	BB80289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB79	CTTTAATTACAGCGTTTTTATTGTTGT	151.23887
BB80290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81831	WGS84	BB80290	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80290_Cmae_Bot2011_COI	GB77	1	BB80290_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80290	Sample	BB80290	targeted	Absent	Sample	Cmae_Bot2011_COI	GB77	COI	45	BB80290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB77	ATGAACAGTCTATCCTCCTTTAG	151.24192
BB80290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81831	WGS84	BB80290	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80290_Msal_Bot2012_COI	GB77	1	BB80290_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80290	Sample	BB80290	targeted	Absent	Sample	Msal_Bot2012_COI	GB77	COI	45	BB80290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB77	GYTAGTTCCRATGATGTTAGCTG	151.24192
BB80292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82116	WGS84	BB80292	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80292_Aamu_Bax2006_COI	GB74	1	BB80292_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80292	Sample	BB80292	targeted	Absent	Sample	Aamu_Bax2006_COI	GB74	COI	45	BB80292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB74	GCACAACCGGGATCTTTACTTC	151.24266
BB80292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82116	WGS84	BB80292	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80292_Pcan_Bot2011_IGS	GB74	1	BB80292_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80292	Sample	BB80292	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB74	IGS	45	BB80292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB74	CGTAATCCTCAGTACTGGCTA	151.24266
BB80292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82116	WGS84	BB80292	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80292_Sspa_Oph2007_COI	GB74	1	BB80292_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80292	Sample	BB80292	targeted	Absent	Sample	Sspa_Oph2007_COI	GB74	COI	45	BB80292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB74	GAAGGCCTTCCGATGATC	151.24266
BB80292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82116	WGS84	BB80292	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80292_Upin_Bot2011_COI	GB74	1	BB80292_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80292	Sample	BB80292	targeted	Absent	Sample	Upin_Bot2011_COI	GB74	COI	45	BB80292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB74	CTTTAATTACAGCGTTTTTATTGTTGT	151.24266
BB80293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82236	WGS84	BB80293	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80293_Mgig_Bot2012_COI	GB73	1	BB80293_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80293	Sample	BB80293	targeted	Absent	Sample	Mgig_Bot2012_COI	GB73	COI	45	BB80293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB73	TCTTATTCGTTGGAGACTTTATAACCCT	151.24253
BB80294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82273	WGS84	BB80294	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80294_Cmae_Bot2011_COI	GB69	1	BB80294_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80294	Sample	BB80294	targeted	Absent	Sample	Cmae_Bot2011_COI	GB69	COI	45	BB80294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB69	ATGAACAGTCTATCCTCCTTTAG	151.24409
BB80294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82273	WGS84	BB80294	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80294_Msal_Bot2012_COI	GB69	1	BB80294_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80294	Sample	BB80294	targeted	Absent	Sample	Msal_Bot2012_COI	GB69	COI	45	BB80294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB69	GYTAGTTCCRATGATGTTAGCTG	151.24409
BB80296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82437	WGS84	BB80296	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80296_Aamu_Bax2006_COI	GB66	1	BB80296_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80296	Sample	BB80296	targeted	Absent	Sample	Aamu_Bax2006_COI	GB66	COI	45	BB80296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB66	GCACAACCGGGATCTTTACTTC	151.24506
BB80296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82437	WGS84	BB80296	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80296_Pcan_Bot2011_IGS	GB66	1	BB80296_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80296	Sample	BB80296	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB66	IGS	45	BB80296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB66	CGTAATCCTCAGTACTGGCTA	151.24506
BB80296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82437	WGS84	BB80296	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80296_Sspa_Oph2007_COI	GB66	1	BB80296_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80296	Sample	BB80296	targeted	Absent	Sample	Sspa_Oph2007_COI	GB66	COI	45	BB80296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB66	GAAGGCCTTCCGATGATC	151.24506
BB80296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82437	WGS84	BB80296	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80296_Upin_Bot2011_COI	GB66	1	BB80296_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80296	Sample	BB80296	targeted	Absent	Sample	Upin_Bot2011_COI	GB66	COI	45	BB80296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB66	CTTTAATTACAGCGTTTTTATTGTTGT	151.24506
BB80297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82526	WGS84	BB80297	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80297_Mgig_Bot2012_COI	GB64	1	BB80297_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80297	Sample	BB80297	targeted	Absent	Sample	Mgig_Bot2012_COI	GB64	COI	45	BB80297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB64	TCTTATTCGTTGGAGACTTTATAACCCT	151.24567
BB80298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8268	WGS84	BB80298	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80298_Cmae_Bot2011_COI	GB34	1	BB80298_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80298	Sample	BB80298	targeted	Absent	Sample	Cmae_Bot2011_COI	GB34	COI	45	BB80298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB34	ATGAACAGTCTATCCTCCTTTAG	151.24598
BB80298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8268	WGS84	BB80298	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80298_Msal_Bot2012_COI	GB34	1	BB80298_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80298	Sample	BB80298	targeted	Absent	Sample	Msal_Bot2012_COI	GB34	COI	45	BB80298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB34	GYTAGTTCCRATGATGTTAGCTG	151.24598
BB80300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82617	WGS84	BB80300	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80300_Aamu_Bax2006_COI	GB31	1	BB80300_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80300	Sample	BB80300	targeted	Absent	Sample	Aamu_Bax2006_COI	GB31	COI	45	BB80300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB31	GCACAACCGGGATCTTTACTTC	151.24826
BB80300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82617	WGS84	BB80300	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80300_Pcan_Bot2011_IGS	GB31	1	BB80300_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80300	Sample	BB80300	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB31	IGS	45	BB80300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB31	CGTAATCCTCAGTACTGGCTA	151.24826
BB80300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82617	WGS84	BB80300	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80300_Sspa_Oph2007_COI	GB31	1	BB80300_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80300	Sample	BB80300	targeted	Absent	Sample	Sspa_Oph2007_COI	GB31	COI	45	BB80300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB31	GAAGGCCTTCCGATGATC	151.24826
BB80300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82617	WGS84	BB80300	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80300_Upin_Bot2011_COI	GB31	1	BB80300_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80300	Sample	BB80300	targeted	Absent	Sample	Upin_Bot2011_COI	GB31	COI	45	BB80300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB31	CTTTAATTACAGCGTTTTTATTGTTGT	151.24826
BB80301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82699	WGS84	BB80301	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80301_Mgig_Bot2012_COI	GB29	1	BB80301_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80301	Sample	BB80301	targeted	Absent	Sample	Mgig_Bot2012_COI	GB29	COI	45	BB80301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB29	TCTTATTCGTTGGAGACTTTATAACCCT	151.24773
BB80302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81859	WGS84	BB80302	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80302_Cmae_Bot2011_COI	GB109	1	BB80302_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80302	Sample	BB80302	targeted	Absent	Sample	Cmae_Bot2011_COI	GB109	COI	45	BB80302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB109	ATGAACAGTCTATCCTCCTTTAG	151.23987
BB80302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81859	WGS84	BB80302	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80302_Msal_Bot2012_COI	GB109	1	BB80302_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80302	Sample	BB80302	targeted	Absent	Sample	Msal_Bot2012_COI	GB109	COI	45	BB80302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB109	GYTAGTTCCRATGATGTTAGCTG	151.23987
BB80304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82071	WGS84	BB80304	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80304_Aamu_Bax2006_COI	GB104	1	BB80304_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80304	Sample	BB80304	targeted	Absent	Sample	Aamu_Bax2006_COI	GB104	COI	45	BB80304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB104	GCACAACCGGGATCTTTACTTC	151.2411
BB80304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82071	WGS84	BB80304	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80304_Pcan_Bot2011_IGS	GB104	1	BB80304_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80304	Sample	BB80304	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB104	IGS	45	BB80304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB104	CGTAATCCTCAGTACTGGCTA	151.2411
BB80304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82071	WGS84	BB80304	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80304_Sspa_Oph2007_COI	GB104	1	BB80304_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80304	Sample	BB80304	targeted	Absent	Sample	Sspa_Oph2007_COI	GB104	COI	45	BB80304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB104	GAAGGCCTTCCGATGATC	151.2411
BB80304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82071	WGS84	BB80304	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80304_Upin_Bot2011_COI	GB104	1	BB80304_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80304	Sample	BB80304	targeted	Absent	Sample	Upin_Bot2011_COI	GB104	COI	45	BB80304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB104	CTTTAATTACAGCGTTTTTATTGTTGT	151.2411
BB80305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81982	WGS84	BB80305	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80305_Mgig_Bot2012_COI	GB103	1	BB80305_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80305	Sample	BB80305	targeted	Absent	Sample	Mgig_Bot2012_COI	GB103	COI	45	BB80305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB103	TCTTATTCGTTGGAGACTTTATAACCCT	151.24166
BB80306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82155	WGS84	BB80306	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80306_Cmae_Bot2011_COI	GB102	1	BB80306_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80306	Sample	BB80306	targeted	Absent	Sample	Cmae_Bot2011_COI	GB102	COI	45	BB80306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB102	ATGAACAGTCTATCCTCCTTTAG	151.24001
BB80306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82155	WGS84	BB80306	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80306_Msal_Bot2012_COI	GB102	1	BB80306_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80306	Sample	BB80306	targeted	Absent	Sample	Msal_Bot2012_COI	GB102	COI	45	BB80306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB102	GYTAGTTCCRATGATGTTAGCTG	151.24001
BB80308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79074	WGS84	BB80308	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80308_Aamu_Bax2006_COI	GB93	1	BB80308_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80308	Sample	BB80308	targeted	Absent	Sample	Aamu_Bax2006_COI	GB93	COI	45	BB80308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB93	GCACAACCGGGATCTTTACTTC	151.21098
BB80308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79074	WGS84	BB80308	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80308_Pcan_Bot2011_IGS	GB93	1	BB80308_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80308	Sample	BB80308	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB93	IGS	45	BB80308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB93	CGTAATCCTCAGTACTGGCTA	151.21098
BB80308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79074	WGS84	BB80308	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80308_Sspa_Oph2007_COI	GB93	1	BB80308_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80308	Sample	BB80308	targeted	Absent	Sample	Sspa_Oph2007_COI	GB93	COI	45	BB80308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB93	GAAGGCCTTCCGATGATC	151.21098
BB80308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79074	WGS84	BB80308	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80308_Upin_Bot2011_COI	GB93	1	BB80308_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80308	Sample	BB80308	targeted	Absent	Sample	Upin_Bot2011_COI	GB93	COI	45	BB80308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB93	CTTTAATTACAGCGTTTTTATTGTTGT	151.21098
BB80309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83073	WGS84	BB80309	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80309_Mgig_Bot2012_COI	GB28	1	BB80309_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80309	Sample	BB80309	targeted	Absent	Sample	Mgig_Bot2012_COI	GB28	COI	45	BB80309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB28	TCTTATTCGTTGGAGACTTTATAACCCT	151.25527
BB80311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83146	WGS84	BB80311	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80311_Aamu_Bax2006_COI	GB26	1	BB80311_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80311	Sample	BB80311	targeted	Absent	Sample	Aamu_Bax2006_COI	GB26	COI	45	BB80311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB26	GCACAACCGGGATCTTTACTTC	151.2596
BB80311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83146	WGS84	BB80311	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80311_Pcan_Bot2011_IGS	GB26	1	BB80311_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80311	Sample	BB80311	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB26	IGS	45	BB80311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB26	CGTAATCCTCAGTACTGGCTA	151.2596
BB80311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83146	WGS84	BB80311	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80311_Sspa_Oph2007_COI	GB26	1	BB80311_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80311	Sample	BB80311	targeted	Absent	Sample	Sspa_Oph2007_COI	GB26	COI	45	BB80311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB26	GAAGGCCTTCCGATGATC	151.2596
BB80311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83146	WGS84	BB80311	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80311_Upin_Bot2011_COI	GB26	1	BB80311_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80311	Sample	BB80311	targeted	Absent	Sample	Upin_Bot2011_COI	GB26	COI	45	BB80311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB26	CTTTAATTACAGCGTTTTTATTGTTGT	151.2596
BB80312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83228	WGS84	BB80312	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80312_Mgig_Bot2012_COI	GB24	1	BB80312_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80312	Sample	BB80312	targeted	Absent	Sample	Mgig_Bot2012_COI	GB24	COI	45	BB80312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB24	TCTTATTCGTTGGAGACTTTATAACCCT	151.26328
BB80313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83295	WGS84	BB80313	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80313_Cmae_Bot2011_COI	GB23	1	BB80313_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80313	Sample	BB80313	targeted	Absent	Sample	Cmae_Bot2011_COI	GB23	COI	45	BB80313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB23	ATGAACAGTCTATCCTCCTTTAG	151.2651
BB80313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83295	WGS84	BB80313	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80313_Msal_Bot2012_COI	GB23	1	BB80313_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80313	Sample	BB80313	targeted	Absent	Sample	Msal_Bot2012_COI	GB23	COI	45	BB80313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB23	GYTAGTTCCRATGATGTTAGCTG	151.2651
BB80315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83503	WGS84	BB80315	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80315_Aamu_Bax2006_COI	GB18	1	BB80315_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80315	Sample	BB80315	targeted	Absent	Sample	Aamu_Bax2006_COI	GB18	COI	45	BB80315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB18	GCACAACCGGGATCTTTACTTC	151.27135
BB80315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83503	WGS84	BB80315	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80315_Pcan_Bot2011_IGS	GB18	1	BB80315_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80315	Sample	BB80315	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB18	IGS	45	BB80315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB18	CGTAATCCTCAGTACTGGCTA	151.27135
BB80315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83503	WGS84	BB80315	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80315_Sspa_Oph2007_COI	GB18	1	BB80315_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80315	Sample	BB80315	targeted	Absent	Sample	Sspa_Oph2007_COI	GB18	COI	45	BB80315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB18	GAAGGCCTTCCGATGATC	151.27135
BB80315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83503	WGS84	BB80315	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80315_Upin_Bot2011_COI	GB18	1	BB80315_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80315	Sample	BB80315	targeted	Absent	Sample	Upin_Bot2011_COI	GB18	COI	45	BB80315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB18	CTTTAATTACAGCGTTTTTATTGTTGT	151.27135
BB80316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85172	WGS84	BB80316	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80316_Mgig_Bot2012_COI	GB13	1	BB80316_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80316	Sample	BB80316	targeted	Absent	Sample	Mgig_Bot2012_COI	GB13	COI	45	BB80316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB13	TCTTATTCGTTGGAGACTTTATAACCCT	151.30872
BB80317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8531	WGS84	BB80317	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80317_Cmae_Bot2011_COI	GB12	1	BB80317_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80317	Sample	BB80317	targeted	Absent	Sample	Cmae_Bot2011_COI	GB12	COI	45	BB80317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB12	ATGAACAGTCTATCCTCCTTTAG	151.30906
BB80317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8531	WGS84	BB80317	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80317_Msal_Bot2012_COI	GB12	1	BB80317_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80317	Sample	BB80317	targeted	Absent	Sample	Msal_Bot2012_COI	GB12	COI	45	BB80317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB12	GYTAGTTCCRATGATGTTAGCTG	151.30906
BB80320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85326	WGS84	BB80320	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80320_Cmae_Bot2011_COI	GB6	1	BB80320_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80320	Sample	BB80320	targeted	Absent	Sample	Cmae_Bot2011_COI	GB6	COI	45	BB80320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB6	ATGAACAGTCTATCCTCCTTTAG	151.31809
BB80320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85326	WGS84	BB80320	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80320_Msal_Bot2012_COI	GB6	1	BB80320_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80320	Sample	BB80320	targeted	Absent	Sample	Msal_Bot2012_COI	GB6	COI	45	BB80320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB6	GYTAGTTCCRATGATGTTAGCTG	151.31809
BB80323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8559	WGS84	BB80323	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80323_Mgig_Bot2012_COI	GB2	1	BB80323_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80323	Sample	BB80323	targeted	Absent	Sample	Mgig_Bot2012_COI	GB2	COI	45	BB80323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB2	TCTTATTCGTTGGAGACTTTATAACCCT	151.32319
BB80477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42858	WGS84	BB80477	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80477_Mgig_Bot2012_COI	BB45	1	BB80477_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80477	Sample	BB80477	targeted	Absent	Sample	Mgig_Bot2012_COI	BB45	COI	45	BB80477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB45	TCTTATTCGTTGGAGACTTTATAACCCT	153.12175
BB80478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40392	WGS84	BB80478	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80478_Cmae_Bot2011_COI	BB60	1	BB80478_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80478	Sample	BB80478	targeted	Absent	Sample	Cmae_Bot2011_COI	BB60	COI	45	BB80478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB60	ATGAACAGTCTATCCTCCTTTAG	153.15318
BB80478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40392	WGS84	BB80478	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80478_Msal_Bot2012_COI	BB60	1	BB80478_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80478	Sample	BB80478	targeted	Absent	Sample	Msal_Bot2012_COI	BB60	COI	45	BB80478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB60	GYTAGTTCCRATGATGTTAGCTG	153.15318
BB80480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44671	WGS84	BB80480	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80480_Mgig_Bot2012_COI	BB40	1	BB80480_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80480	Sample	BB80480	targeted	Absent	Sample	Mgig_Bot2012_COI	BB40	COI	45	BB80480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB40	TCTTATTCGTTGGAGACTTTATAACCCT	153.07953
BB80481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38453	WGS84	BB80481	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80481_Cmae_Bot2011_COI	BB20	1	BB80481_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80481	Sample	BB80481	targeted	Absent	Sample	Cmae_Bot2011_COI	BB20	COI	45	BB80481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB20	ATGAACAGTCTATCCTCCTTTAG	153.15992
BB80481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38453	WGS84	BB80481	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80481_Msal_Bot2012_COI	BB20	1	BB80481_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80481	Sample	BB80481	targeted	Absent	Sample	Msal_Bot2012_COI	BB20	COI	45	BB80481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB20	GYTAGTTCCRATGATGTTAGCTG	153.15992
BB80483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41377	WGS84	BB80483	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80483_Aamu_Bax2006_COI	BB57	1	BB80483_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80483	Sample	BB80483	targeted	Absent	Sample	Aamu_Bax2006_COI	BB57	COI	45	BB80483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB57	GCACAACCGGGATCTTTACTTC	153.14357
BB80483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41377	WGS84	BB80483	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80483_Pcan_Bot2011_IGS	BB57	1	BB80483_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80483	Sample	BB80483	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB57	IGS	45	BB80483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB57	CGTAATCCTCAGTACTGGCTA	153.14357
BB80483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41377	WGS84	BB80483	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80483_Sspa_Oph2007_COI	BB57	1	BB80483_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80483	Sample	BB80483	targeted	Absent	Sample	Sspa_Oph2007_COI	BB57	COI	45	BB80483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB57	GAAGGCCTTCCGATGATC	153.14357
BB80483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41377	WGS84	BB80483	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80483_Upin_Bot2011_COI	BB57	1	BB80483_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80483	Sample	BB80483	targeted	Absent	Sample	Upin_Bot2011_COI	BB57	COI	45	BB80483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB57	CTTTAATTACAGCGTTTTTATTGTTGT	153.14357
BB80484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41854	WGS84	BB80484	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80484_Mgig_Bot2012_COI	BB56	1	BB80484_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80484	Sample	BB80484	targeted	Absent	Sample	Mgig_Bot2012_COI	BB56	COI	45	BB80484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB56	TCTTATTCGTTGGAGACTTTATAACCCT	153.13951
BB80485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42242	WGS84	BB80485	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80485_Cmae_Bot2011_COI	BB54	1	BB80485_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80485	Sample	BB80485	targeted	Absent	Sample	Cmae_Bot2011_COI	BB54	COI	45	BB80485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB54	ATGAACAGTCTATCCTCCTTTAG	153.13425
BB80485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42242	WGS84	BB80485	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80485_Msal_Bot2012_COI	BB54	1	BB80485_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80485	Sample	BB80485	targeted	Absent	Sample	Msal_Bot2012_COI	BB54	COI	45	BB80485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB54	GYTAGTTCCRATGATGTTAGCTG	153.13425
BB80487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42763	WGS84	BB80487	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80487_Aamu_Bax2006_COI	BB52	1	BB80487_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80487	Sample	BB80487	targeted	Absent	Sample	Aamu_Bax2006_COI	BB52	COI	45	BB80487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB52	GCACAACCGGGATCTTTACTTC	153.12369
BB80487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42763	WGS84	BB80487	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80487_Pcan_Bot2011_IGS	BB52	1	BB80487_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80487	Sample	BB80487	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB52	IGS	45	BB80487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB52	CGTAATCCTCAGTACTGGCTA	153.12369
BB80487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42763	WGS84	BB80487	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80487_Sspa_Oph2007_COI	BB52	1	BB80487_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80487	Sample	BB80487	targeted	Absent	Sample	Sspa_Oph2007_COI	BB52	COI	45	BB80487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB52	GAAGGCCTTCCGATGATC	153.12369
BB80487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42763	WGS84	BB80487	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80487_Upin_Bot2011_COI	BB52	1	BB80487_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80487	Sample	BB80487	targeted	Absent	Sample	Upin_Bot2011_COI	BB52	COI	45	BB80487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB52	CTTTAATTACAGCGTTTTTATTGTTGT	153.12369
BB80488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4305	WGS84	BB80488	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80488_Mgig_Bot2012_COI	BB47	1	BB80488_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80488	Sample	BB80488	targeted	Absent	Sample	Mgig_Bot2012_COI	BB47	COI	45	BB80488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB47	TCTTATTCGTTGGAGACTTTATAACCCT	153.12348
BB80489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.43049	WGS84	BB80489	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80489_Cmae_Bot2011_COI	BB44	1	BB80489_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80489	Sample	BB80489	targeted	Absent	Sample	Cmae_Bot2011_COI	BB44	COI	45	BB80489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB44	ATGAACAGTCTATCCTCCTTTAG	153.11875
BB80489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.43049	WGS84	BB80489	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80489_Msal_Bot2012_COI	BB44	1	BB80489_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80489	Sample	BB80489	targeted	Absent	Sample	Msal_Bot2012_COI	BB44	COI	45	BB80489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB44	GYTAGTTCCRATGATGTTAGCTG	153.11875
BB80490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40702	WGS84	BB80490	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80490_Aamu_Bax2006_COI	BB62	1	BB80490_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80490	Sample	BB80490	targeted	Absent	Sample	Aamu_Bax2006_COI	BB62	COI	45	BB80490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB62	GCACAACCGGGATCTTTACTTC	153.15063
BB80490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40702	WGS84	BB80490	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80490_Pcan_Bot2011_IGS	BB62	1	BB80490_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80490	Sample	BB80490	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB62	IGS	45	BB80490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB62	CGTAATCCTCAGTACTGGCTA	153.15063
BB80490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40702	WGS84	BB80490	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80490_Sspa_Oph2007_COI	BB62	1	BB80490_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80490	Sample	BB80490	targeted	Absent	Sample	Sspa_Oph2007_COI	BB62	COI	45	BB80490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB62	GAAGGCCTTCCGATGATC	153.15063
BB80490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40702	WGS84	BB80490	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80490_Upin_Bot2011_COI	BB62	1	BB80490_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80490	Sample	BB80490	targeted	Absent	Sample	Upin_Bot2011_COI	BB62	COI	45	BB80490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB62	CTTTAATTACAGCGTTTTTATTGTTGT	153.15063
BB80491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4011	WGS84	BB80491	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80491_Mgig_Bot2012_COI	BB58	1	BB80491_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80491	Sample	BB80491	targeted	Absent	Sample	Mgig_Bot2012_COI	BB58	COI	45	BB80491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB58	TCTTATTCGTTGGAGACTTTATAACCCT	153.15819
BB80492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40135	WGS84	BB80492	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80492_Cmae_Bot2011_COI	BB27	1	BB80492_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80492	Sample	BB80492	targeted	Absent	Sample	Cmae_Bot2011_COI	BB27	COI	45	BB80492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB27	ATGAACAGTCTATCCTCCTTTAG	153.16103
BB80492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40135	WGS84	BB80492	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80492_Msal_Bot2012_COI	BB27	1	BB80492_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80492	Sample	BB80492	targeted	Absent	Sample	Msal_Bot2012_COI	BB27	COI	45	BB80492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB27	GYTAGTTCCRATGATGTTAGCTG	153.16103
BB80494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44631	WGS84	BB80494	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80494_Aamu_Bax2006_COI	BB39	1	BB80494_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80494	Sample	BB80494	targeted	Absent	Sample	Aamu_Bax2006_COI	BB39	COI	45	BB80494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB39	GCACAACCGGGATCTTTACTTC	153.0775
BB80494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44631	WGS84	BB80494	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80494_Pcan_Bot2011_IGS	BB39	1	BB80494_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80494	Sample	BB80494	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB39	IGS	45	BB80494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB39	CGTAATCCTCAGTACTGGCTA	153.0775
BB80494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44631	WGS84	BB80494	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80494_Sspa_Oph2007_COI	BB39	1	BB80494_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80494	Sample	BB80494	targeted	Absent	Sample	Sspa_Oph2007_COI	BB39	COI	45	BB80494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB39	GAAGGCCTTCCGATGATC	153.0775
BB80494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44631	WGS84	BB80494	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80494_Upin_Bot2011_COI	BB39	1	BB80494_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80494	Sample	BB80494	targeted	Absent	Sample	Upin_Bot2011_COI	BB39	COI	45	BB80494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB39	CTTTAATTACAGCGTTTTTATTGTTGT	153.0775
BB80495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44492	WGS84	BB80495	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80495_Mgig_Bot2012_COI	BB37	1	BB80495_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80495	Sample	BB80495	targeted	Absent	Sample	Mgig_Bot2012_COI	BB37	COI	45	BB80495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB37	TCTTATTCGTTGGAGACTTTATAACCCT	153.07984
BB80496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44558	WGS84	BB80496	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80496_Cmae_Bot2011_COI	BB36	1	BB80496_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80496	Sample	BB80496	targeted	Absent	Sample	Cmae_Bot2011_COI	BB36	COI	45	BB80496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB36	ATGAACAGTCTATCCTCCTTTAG	153.07838
BB80496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44558	WGS84	BB80496	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80496_Msal_Bot2012_COI	BB36	1	BB80496_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80496	Sample	BB80496	targeted	Absent	Sample	Msal_Bot2012_COI	BB36	COI	45	BB80496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB36	GYTAGTTCCRATGATGTTAGCTG	153.07838
BB80498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44397	WGS84	BB80498	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80498_Aamu_Bax2006_COI	BB31	1	BB80498_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80498	Sample	BB80498	targeted	Absent	Sample	Aamu_Bax2006_COI	BB31	COI	45	BB80498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB31	GCACAACCGGGATCTTTACTTC	153.07629
BB80498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44397	WGS84	BB80498	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80498_Pcan_Bot2011_IGS	BB31	1	BB80498_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80498	Sample	BB80498	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB31	IGS	45	BB80498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB31	CGTAATCCTCAGTACTGGCTA	153.07629
BB80498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44397	WGS84	BB80498	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80498_Sspa_Oph2007_COI	BB31	1	BB80498_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80498	Sample	BB80498	targeted	Absent	Sample	Sspa_Oph2007_COI	BB31	COI	45	BB80498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB31	GAAGGCCTTCCGATGATC	153.07629
BB80498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44397	WGS84	BB80498	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80498_Upin_Bot2011_COI	BB31	1	BB80498_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80498	Sample	BB80498	targeted	Absent	Sample	Upin_Bot2011_COI	BB31	COI	45	BB80498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB31	CTTTAATTACAGCGTTTTTATTGTTGT	153.07629
BB80499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44256	WGS84	BB80499	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80499_Mgig_Bot2012_COI	BB29	1	BB80499_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80499	Sample	BB80499	targeted	Absent	Sample	Mgig_Bot2012_COI	BB29	COI	45	BB80499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB29	TCTTATTCGTTGGAGACTTTATAACCCT	153.07224
BB80500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.3698	WGS84	BB80500	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80500_Cmae_Bot2011_COI	BB9	1	BB80500_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80500	Sample	BB80500	targeted	Absent	Sample	Cmae_Bot2011_COI	BB9	COI	45	BB80500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB9	ATGAACAGTCTATCCTCCTTTAG	153.17124
BB80500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.3698	WGS84	BB80500	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80500_Msal_Bot2012_COI	BB9	1	BB80500_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80500	Sample	BB80500	targeted	Absent	Sample	Msal_Bot2012_COI	BB9	COI	45	BB80500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB9	GYTAGTTCCRATGATGTTAGCTG	153.17124
BB80502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36487	WGS84	BB80502	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80502_Aamu_Bax2006_COI	BB6	1	BB80502_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80502	Sample	BB80502	targeted	Absent	Sample	Aamu_Bax2006_COI	BB6	COI	45	BB80502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB6	GCACAACCGGGATCTTTACTTC	153.1748
BB80502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36487	WGS84	BB80502	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80502_Pcan_Bot2011_IGS	BB6	1	BB80502_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80502	Sample	BB80502	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB6	IGS	45	BB80502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB6	CGTAATCCTCAGTACTGGCTA	153.1748
BB80502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36487	WGS84	BB80502	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80502_Sspa_Oph2007_COI	BB6	1	BB80502_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80502	Sample	BB80502	targeted	Absent	Sample	Sspa_Oph2007_COI	BB6	COI	45	BB80502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB6	GAAGGCCTTCCGATGATC	153.1748
BB80502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36487	WGS84	BB80502	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80502_Upin_Bot2011_COI	BB6	1	BB80502_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80502	Sample	BB80502	targeted	Absent	Sample	Upin_Bot2011_COI	BB6	COI	45	BB80502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB6	CTTTAATTACAGCGTTTTTATTGTTGT	153.1748
BB80503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36134	WGS84	BB80503	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80503_Mgig_Bot2012_COI	BB4	1	BB80503_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80503	Sample	BB80503	targeted	Absent	Sample	Mgig_Bot2012_COI	BB4	COI	45	BB80503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB4	TCTTATTCGTTGGAGACTTTATAACCCT	153.17733
BB80504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.39048	WGS84	BB80504	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80504_Cmae_Bot2011_COI	BB24	1	BB80504_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80504	Sample	BB80504	targeted	Absent	Sample	Cmae_Bot2011_COI	BB24	COI	45	BB80504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB24	ATGAACAGTCTATCCTCCTTTAG	153.15714
BB80504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.39048	WGS84	BB80504	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80504_Msal_Bot2012_COI	BB24	1	BB80504_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80504	Sample	BB80504	targeted	Absent	Sample	Msal_Bot2012_COI	BB24	COI	45	BB80504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB24	GYTAGTTCCRATGATGTTAGCTG	153.15714
BB87783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84762	WGS84	BB87783	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87783_Aamu_Bax2006_COI	T11-01	1	BB87783_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87783	Sample	BB87783	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-01	COI	45	BB87783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-01	GCACAACCGGGATCTTTACTTC	147.87521
BB87783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84762	WGS84	BB87783	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87783_Pcan_Bot2011_IGS	T11-01	1	BB87783_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87783	Sample	BB87783	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-01	IGS	45	BB87783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-01	CGTAATCCTCAGTACTGGCTA	147.87521
BB87783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84762	WGS84	BB87783	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87783_Sspa_Oph2007_COI	T11-01	1	BB87783_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87783	Sample	BB87783	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-01	COI	45	BB87783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-01	GAAGGCCTTCCGATGATC	147.87521
BB87783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84762	WGS84	BB87783	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87783_Upin_Bot2011_COI	T11-01	1	BB87783_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87783	Sample	BB87783	targeted	Absent	Sample	Upin_Bot2011_COI	T11-01	COI	45	BB87783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.87521
BB87784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85098	WGS84	BB87784	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87784_Cjap_Sim2018_COI	T11-02	1	BB87784_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87784	Sample	BB87784	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-02	COI	45	BB87784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87402
BB87784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85098	WGS84	BB87784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.207	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	27/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87784_Mgig_Bot2012_COI	T11-02	1	BB87784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87784	Sample	BB87784	targeted	Present	Sample	Mgig_Bot2012_COI	T11-02	COI	45	BB87784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.87402
BB87785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85535	WGS84	BB87785	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87785_Cmae_Bot2011_COI	T11-03	1	BB87785_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87785	Sample	BB87785	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-03	COI	45	BB87785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-03	ATGAACAGTCTATCCTCCTTTAG	147.87278
BB87787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96278	WGS84	BB87787	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.039	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87787_Aamu_Bax2006_COI	T11-05	1	BB87787_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87787	Sample	BB87787	targeted	Present	Sample	Aamu_Bax2006_COI	T11-05	COI	45	BB87787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-05	GCACAACCGGGATCTTTACTTC	147.53883
BB87787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96278	WGS84	BB87787	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87787_Pcan_Bot2011_IGS	T11-05	1	BB87787_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87787	Sample	BB87787	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-05	IGS	45	BB87787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-05	CGTAATCCTCAGTACTGGCTA	147.53883
BB87787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96278	WGS84	BB87787	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87787_Sspa_Oph2007_COI	T11-05	1	BB87787_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87787	Sample	BB87787	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-05	COI	45	BB87787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-05	GAAGGCCTTCCGATGATC	147.53883
BB87787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96278	WGS84	BB87787	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87787_Upin_Bot2011_COI	T11-05	1	BB87787_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87787	Sample	BB87787	targeted	Absent	Sample	Upin_Bot2011_COI	T11-05	COI	45	BB87787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.53883
BB87788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.95998	WGS84	BB87788	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87788_Cjap_Sim2018_COI	T11-06	1	BB87788_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87788	Sample	BB87788	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-06	COI	45	BB87788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52818
BB87788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.95998	WGS84	BB87788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.869	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	29/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87788_Mgig_Bot2012_COI	T11-06	1	BB87788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87788	Sample	BB87788	targeted	Present	Sample	Mgig_Bot2012_COI	T11-06	COI	45	BB87788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.52818
BB87789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96348	WGS84	BB87789	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87789_Cmae_Bot2011_COI	T11-07	1	BB87789_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87789	Sample	BB87789	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-07	COI	45	BB87789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-07	ATGAACAGTCTATCCTCCTTTAG	147.51931
BB87790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82515	WGS84	BB87790	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87790_Aamu_Bax2006_COI	T11-08	1	BB87790_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87790	Sample	BB87790	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-08	COI	45	BB87790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-08	GCACAACCGGGATCTTTACTTC	147.3078
BB87790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82515	WGS84	BB87790	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87790_Pcan_Bot2011_IGS	T11-08	1	BB87790_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87790	Sample	BB87790	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-08	IGS	45	BB87790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-08	CGTAATCCTCAGTACTGGCTA	147.3078
BB87790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82515	WGS84	BB87790	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87790_Sspa_Oph2007_COI	T11-08	1	BB87790_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87790	Sample	BB87790	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-08	COI	45	BB87790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-08	GAAGGCCTTCCGATGATC	147.3078
BB87790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82515	WGS84	BB87790	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87790_Upin_Bot2011_COI	T11-08	1	BB87790_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87790	Sample	BB87790	targeted	Absent	Sample	Upin_Bot2011_COI	T11-08	COI	45	BB87790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.3078
BB87791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8275	WGS84	BB87791	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87791_Cjap_Sim2018_COI	T11-09	1	BB87791_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87791	Sample	BB87791	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-09	COI	45	BB87791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30215
BB87791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.8275	WGS84	BB87791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.136	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87791_Mgig_Bot2012_COI	T11-09	1	BB87791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87791	Sample	BB87791	targeted	Present	Sample	Mgig_Bot2012_COI	T11-09	COI	45	BB87791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.30215
BB87792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82887	WGS84	BB87792	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.393	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87792_Cmae_Bot2011_COI	T11-10	1	BB87792_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87792	Sample	BB87792	targeted	Present	Sample	Cmae_Bot2011_COI	T11-10	COI	45	BB87792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-10	ATGAACAGTCTATCCTCCTTTAG	147.30192
BB87794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88265	WGS84	BB87794	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	41.149	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87794_Aamu_Bax2006_COI	T11-13	1	BB87794_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87794	Sample	BB87794	targeted	Present	Sample	Aamu_Bax2006_COI	T11-13	COI	45	BB87794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-13	GCACAACCGGGATCTTTACTTC	147.33628
BB87794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88265	WGS84	BB87794	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87794_Pcan_Bot2011_IGS	T11-13	1	BB87794_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87794	Sample	BB87794	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-13	IGS	45	BB87794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-13	CGTAATCCTCAGTACTGGCTA	147.33628
BB87794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88265	WGS84	BB87794	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87794_Sspa_Oph2007_COI	T11-13	1	BB87794_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87794	Sample	BB87794	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-13	COI	45	BB87794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-13	GAAGGCCTTCCGATGATC	147.33628
BB87794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88265	WGS84	BB87794	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87794_Upin_Bot2011_COI	T11-13	1	BB87794_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87794	Sample	BB87794	targeted	Absent	Sample	Upin_Bot2011_COI	T11-13	COI	45	BB87794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.33628
BB87795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88453	WGS84	BB87795	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87795_Cjap_Sim2018_COI	T11-12	1	BB87795_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87795	Sample	BB87795	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-12	COI	45	BB87795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33611
BB87795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88453	WGS84	BB87795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.015	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87795_Mgig_Bot2012_COI	T11-12	1	BB87795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87795	Sample	BB87795	targeted	Present	Sample	Mgig_Bot2012_COI	T11-12	COI	45	BB87795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.33611
BB87796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88082	WGS84	BB87796	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87796_Cmae_Bot2011_COI	T11-14	1	BB87796_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87796	Sample	BB87796	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-14	COI	45	BB87796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-14	ATGAACAGTCTATCCTCCTTTAG	147.3422
BB87798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87305	WGS84	BB87798	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87798_Aamu_Bax2006_COI	T11-16	1	BB87798_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87798	Sample	BB87798	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-16	COI	45	BB87798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-16	GCACAACCGGGATCTTTACTTC	147.3363
BB87798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87305	WGS84	BB87798	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87798_Pcan_Bot2011_IGS	T11-16	1	BB87798_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87798	Sample	BB87798	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-16	IGS	45	BB87798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-16	CGTAATCCTCAGTACTGGCTA	147.3363
BB87798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87305	WGS84	BB87798	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87798_Sspa_Oph2007_COI	T11-16	1	BB87798_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87798	Sample	BB87798	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-16	COI	45	BB87798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-16	GAAGGCCTTCCGATGATC	147.3363
BB87798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87305	WGS84	BB87798	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87798_Upin_Bot2011_COI	T11-16	1	BB87798_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87798	Sample	BB87798	targeted	Absent	Sample	Upin_Bot2011_COI	T11-16	COI	45	BB87798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.3363
BB87799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84645	WGS84	BB87799	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87799_Cjap_Sim2018_COI	S7-02	1	BB87799_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87799	Sample	BB87799	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-02	COI	45	BB87799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49705
BB87799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84645	WGS84	BB87799	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87799_Mgig_Bot2012_COI	S7-02	1	BB87799_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87799	Sample	BB87799	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-02	COI	45	BB87799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.49705
BB87800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87800	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.701	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87800_Cmae_Bot2011_COI	S7-03	1	BB87800_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87800	Sample	BB87800	targeted	Present	Sample	Cmae_Bot2011_COI	S7-03	COI	45	BB87800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-03	ATGAACAGTCTATCCTCCTTTAG	138.49696
BB87803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83649	WGS84	BB87803	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87803_Cjap_Sim2018_COI	S7-06	1	BB87803_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87803	Sample	BB87803	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-06	COI	45	BB87803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50852
BB87803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83649	WGS84	BB87803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87803_Mgig_Bot2012_COI	S7-06	1	BB87803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87803	Sample	BB87803	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-06	COI	45	BB87803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.50852
BB87804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83704	WGS84	BB87804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.439	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87804_Cmae_Bot2011_COI	S7-07	1	BB87804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87804	Sample	BB87804	targeted	Present	Sample	Cmae_Bot2011_COI	S7-07	COI	45	BB87804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-07	ATGAACAGTCTATCCTCCTTTAG	138.50986
BB87808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78381	WGS84	BB87808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.184	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87808_Cmae_Bot2011_COI	S7-11	1	BB87808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87808	Sample	BB87808	targeted	Present	Sample	Cmae_Bot2011_COI	S7-11	COI	45	BB87808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-11	ATGAACAGTCTATCCTCCTTTAG	138.51578
BB87810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78245	WGS84	BB87810	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87810_Cjap_Sim2018_COI	S7-13	1	BB87810_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87810	Sample	BB87810	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-13	COI	45	BB87810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51632
BB87810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78245	WGS84	BB87810	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.383	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87810_Mgig_Bot2012_COI	S7-13	1	BB87810_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87810	Sample	BB87810	targeted	Present	Sample	Mgig_Bot2012_COI	S7-13	COI	45	BB87810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.51632
BB87813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77223	WGS84	BB87813	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87813_Aamu_Bax2006_COI	S7-16	1	BB87813_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87813	Sample	BB87813	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-16	COI	45	BB87813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-16	GCACAACCGGGATCTTTACTTC	138.48596
BB87813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77223	WGS84	BB87813	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87813_Pcan_Bot2011_IGS	S7-16	1	BB87813_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87813	Sample	BB87813	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-16	IGS	45	BB87813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-16	CGTAATCCTCAGTACTGGCTA	138.48596
BB87813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77223	WGS84	BB87813	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.36	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87813_Sspa_Oph2007_COI	S7-16	1	BB87813_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87813	Sample	BB87813	targeted	Present	Sample	Sspa_Oph2007_COI	S7-16	COI	45	BB87813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-16	GAAGGCCTTCCGATGATC	138.48596
BB87813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77223	WGS84	BB87813	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87813_Upin_Bot2011_COI	S7-16	1	BB87813_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87813	Sample	BB87813	targeted	Absent	Sample	Upin_Bot2011_COI	S7-16	COI	45	BB87813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.48596
BB87814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77378	WGS84	BB87814	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87814_Cjap_Sim2018_COI	S7-17	1	BB87814_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87814	Sample	BB87814	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-17	COI	45	BB87814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4857
BB87814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77378	WGS84	BB87814	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.936	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87814_Mgig_Bot2012_COI	S7-17	1	BB87814_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87814	Sample	BB87814	targeted	Present	Sample	Mgig_Bot2012_COI	S7-17	COI	45	BB87814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.4857
BB87815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77396	WGS84	BB87815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87815_Cmae_Bot2011_COI	S7-18	1	BB87815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87815	Sample	BB87815	targeted	Absent	Sample	Cmae_Bot2011_COI	S7-18	COI	45	BB87815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-18	ATGAACAGTCTATCCTCCTTTAG	138.486
BB87818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80928	WGS84	BB87818	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87818_Cjap_Sim2018_COI	S7-21	1	BB87818_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87818	Sample	BB87818	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-21	COI	45	BB87818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-21	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51791
BB87818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.80928	WGS84	BB87818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.958	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87818_Mgig_Bot2012_COI	S7-21	1	BB87818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87818	Sample	BB87818	targeted	Present	Sample	Mgig_Bot2012_COI	S7-21	COI	45	BB87818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-21	TCTTATTCGTTGGAGACTTTATAACCCT	138.51791
BB87819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80999	WGS84	BB87819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.034	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87819_Cmae_Bot2011_COI	S7-22	1	BB87819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87819	Sample	BB87819	targeted	Present	Sample	Cmae_Bot2011_COI	S7-22	COI	45	BB87819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-22	ATGAACAGTCTATCCTCCTTTAG	138.51976
BB87822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81158	WGS84	BB87822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.63	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87822_Cmae_Bot2011_COI	S7-25	1	BB87822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87822	Sample	BB87822	targeted	Present	Sample	Cmae_Bot2011_COI	S7-25	COI	45	BB87822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-25	ATGAACAGTCTATCCTCCTTTAG	138.52358
BB87824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84768	WGS84	BB87824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.102	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87824_Aamu_Bax2006_COI	T12-01	1	BB87824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87824	Sample	BB87824	targeted	Present	Sample	Aamu_Bax2006_COI	T12-01	COI	45	BB87824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-01	GCACAACCGGGATCTTTACTTC	147.8754
BB87824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84768	WGS84	BB87824	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87824_Pcan_Bot2011_IGS	T12-01	1	BB87824_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87824	Sample	BB87824	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-01	IGS	45	BB87824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-01	CGTAATCCTCAGTACTGGCTA	147.8754
BB87824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84768	WGS84	BB87824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87824_Sspa_Oph2007_COI	T12-01	1	BB87824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87824	Sample	BB87824	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-01	COI	45	BB87824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-01	GAAGGCCTTCCGATGATC	147.8754
BB87824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84768	WGS84	BB87824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87824_Upin_Bot2011_COI	T12-01	1	BB87824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87824	Sample	BB87824	targeted	Absent	Sample	Upin_Bot2011_COI	T12-01	COI	45	BB87824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.8754
BB87825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85088	WGS84	BB87825	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87825_Cjap_Sim2018_COI	T12-02	1	BB87825_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87825	Sample	BB87825	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-02	COI	45	BB87825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87377
BB87825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85088	WGS84	BB87825	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	28/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87825_Mgig_Bot2012_COI	T12-02	1	BB87825_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87825	Sample	BB87825	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-02	COI	45	BB87825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.87377
BB87826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85596	WGS84	BB87826	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87826_Cmae_Bot2011_COI	T12-03	1	BB87826_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87826	Sample	BB87826	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-03	COI	45	BB87826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-03	ATGAACAGTCTATCCTCCTTTAG	147.87236
BB87828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88174	WGS84	BB87828	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.038	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87828_Aamu_Bax2006_COI	T12-05	1	BB87828_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87828	Sample	BB87828	targeted	Present	Sample	Aamu_Bax2006_COI	T12-05	COI	45	BB87828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-05	GCACAACCGGGATCTTTACTTC	147.34223
BB87828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88174	WGS84	BB87828	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87828_Pcan_Bot2011_IGS	T12-05	1	BB87828_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87828	Sample	BB87828	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-05	IGS	45	BB87828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-05	CGTAATCCTCAGTACTGGCTA	147.34223
BB87828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88174	WGS84	BB87828	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87828_Sspa_Oph2007_COI	T12-05	1	BB87828_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87828	Sample	BB87828	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-05	COI	45	BB87828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-05	GAAGGCCTTCCGATGATC	147.34223
BB87828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88174	WGS84	BB87828	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.905	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87828_Upin_Bot2011_COI	T12-05	1	BB87828_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87828	Sample	BB87828	targeted	Present	Sample	Upin_Bot2011_COI	T12-05	COI	45	BB87828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.34223
BB87829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87783	WGS84	BB87829	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87829_Cjap_Sim2018_COI	T12-06	1	BB87829_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87829	Sample	BB87829	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-06	COI	45	BB87829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34193
BB87829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87783	WGS84	BB87829	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87829_Mgig_Bot2012_COI	T12-06	1	BB87829_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87829	Sample	BB87829	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-06	COI	45	BB87829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.34193
BB87831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82866	WGS84	BB87831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.874	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87831_Aamu_Bax2006_COI	T12-08	1	BB87831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87831	Sample	BB87831	targeted	Present	Sample	Aamu_Bax2006_COI	T12-08	COI	45	BB87831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-08	GCACAACCGGGATCTTTACTTC	147.30169
BB87831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82866	WGS84	BB87831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87831_Pcan_Bot2011_IGS	T12-08	1	BB87831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87831	Sample	BB87831	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-08	IGS	45	BB87831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-08	CGTAATCCTCAGTACTGGCTA	147.30169
BB87831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82866	WGS84	BB87831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87831_Sspa_Oph2007_COI	T12-08	1	BB87831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87831	Sample	BB87831	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-08	COI	45	BB87831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-08	GAAGGCCTTCCGATGATC	147.30169
BB87831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82866	WGS84	BB87831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87831_Upin_Bot2011_COI	T12-08	1	BB87831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87831	Sample	BB87831	targeted	Absent	Sample	Upin_Bot2011_COI	T12-08	COI	45	BB87831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.30169
BB87832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82729	WGS84	BB87832	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87832_Cjap_Sim2018_COI	T12-09	1	BB87832_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87832	Sample	BB87832	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-09	COI	45	BB87832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30174
BB87832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82729	WGS84	BB87832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87832_Mgig_Bot2012_COI	T12-09	1	BB87832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87832	Sample	BB87832	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-09	COI	45	BB87832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.30174
BB87833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82518	WGS84	BB87833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87833_Cmae_Bot2011_COI	T12-10	1	BB87833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87833	Sample	BB87833	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-10	COI	45	BB87833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-10	ATGAACAGTCTATCCTCCTTTAG	147.30494
BB87835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88464	WGS84	BB87835	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.4	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87835_Aamu_Bax2006_COI	T12-12	1	BB87835_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87835	Sample	BB87835	targeted	Present	Sample	Aamu_Bax2006_COI	T12-12	COI	45	BB87835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-12	GCACAACCGGGATCTTTACTTC	147.33707
BB87835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88464	WGS84	BB87835	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87835_Pcan_Bot2011_IGS	T12-12	1	BB87835_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87835	Sample	BB87835	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-12	IGS	45	BB87835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-12	CGTAATCCTCAGTACTGGCTA	147.33707
BB87835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88464	WGS84	BB87835	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87835_Sspa_Oph2007_COI	T12-12	1	BB87835_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87835	Sample	BB87835	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-12	COI	45	BB87835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-12	GAAGGCCTTCCGATGATC	147.33707
BB87835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88464	WGS84	BB87835	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87835_Upin_Bot2011_COI	T12-12	1	BB87835_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87835	Sample	BB87835	targeted	Absent	Sample	Upin_Bot2011_COI	T12-12	COI	45	BB87835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.33707
BB87836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88287	WGS84	BB87836	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87836_Cjap_Sim2018_COI	T12-13	1	BB87836_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87836	Sample	BB87836	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-13	COI	45	BB87836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33643
BB87836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88287	WGS84	BB87836	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87836_Mgig_Bot2012_COI	T12-13	1	BB87836_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87836	Sample	BB87836	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-13	COI	45	BB87836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.33643
BB87837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83653	WGS84	BB87837	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.174	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87837_Cmae_Bot2011_COI	S8-25	1	BB87837_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87837	Sample	BB87837	targeted	Present	Sample	Cmae_Bot2011_COI	S8-25	COI	45	BB87837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-25	ATGAACAGTCTATCCTCCTTTAG	138.50737
BB87839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83645	WGS84	BB87839	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87839_Aamu_Bax2006_COI	S8-24	1	BB87839_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87839	Sample	BB87839	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-24	COI	45	BB87839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-24	GCACAACCGGGATCTTTACTTC	138.50846
BB87839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83645	WGS84	BB87839	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87839_Pcan_Bot2011_IGS	S8-24	1	BB87839_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87839	Sample	BB87839	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-24	IGS	45	BB87839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-24	CGTAATCCTCAGTACTGGCTA	138.50846
BB87839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83645	WGS84	BB87839	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.852	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87839_Sspa_Oph2007_COI	S8-24	1	BB87839_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87839	Sample	BB87839	targeted	Present	Sample	Sspa_Oph2007_COI	S8-24	COI	45	BB87839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-24	GAAGGCCTTCCGATGATC	138.50846
BB87839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83645	WGS84	BB87839	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87839_Upin_Bot2011_COI	S8-24	1	BB87839_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87839	Sample	BB87839	targeted	Absent	Sample	Upin_Bot2011_COI	S8-24	COI	45	BB87839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-24	CTTTAATTACAGCGTTTTTATTGTTGT	138.50846
BB87840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96205	WGS84	BB87840	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87840_Cmae_Bot2011_COI	T13-01	1	BB87840_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87840	Sample	BB87840	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-01	COI	45	BB87840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-01	ATGAACAGTCTATCCTCCTTTAG	147.53986
BB87842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96236	WGS84	BB87842	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.801	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87842_Aamu_Bax2006_COI	T13-03	1	BB87842_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87842	Sample	BB87842	targeted	Present	Sample	Aamu_Bax2006_COI	T13-03	COI	45	BB87842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-03	GCACAACCGGGATCTTTACTTC	147.51954
BB87842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96236	WGS84	BB87842	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87842_Pcan_Bot2011_IGS	T13-03	1	BB87842_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87842	Sample	BB87842	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-03	IGS	45	BB87842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-03	CGTAATCCTCAGTACTGGCTA	147.51954
BB87842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96236	WGS84	BB87842	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87842_Sspa_Oph2007_COI	T13-03	1	BB87842_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87842	Sample	BB87842	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-03	COI	45	BB87842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-03	GAAGGCCTTCCGATGATC	147.51954
BB87842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96236	WGS84	BB87842	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87842_Upin_Bot2011_COI	T13-03	1	BB87842_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87842	Sample	BB87842	targeted	Absent	Sample	Upin_Bot2011_COI	T13-03	COI	45	BB87842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.51954
BB87843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84846	WGS84	BB87843	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87843_Cjap_Sim2018_COI	T13-04	1	BB87843_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87843	Sample	BB87843	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-04	COI	45	BB87843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87427
BB87843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.84846	WGS84	BB87843	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87843_Mgig_Bot2012_COI	T13-04	1	BB87843_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87843	Sample	BB87843	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-04	COI	45	BB87843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.87427
BB87844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85093	WGS84	BB87844	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87844_Cmae_Bot2011_COI	T13-05	1	BB87844_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87844	Sample	BB87844	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-05	COI	45	BB87844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-05	ATGAACAGTCTATCCTCCTTTAG	147.87402
BB87846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88938	WGS84	BB87846	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.689	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87846_Aamu_Bax2006_COI	T13-07	1	BB87846_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87846	Sample	BB87846	targeted	Present	Sample	Aamu_Bax2006_COI	T13-07	COI	45	BB87846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-07	GCACAACCGGGATCTTTACTTC	147.81479
BB87846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88938	WGS84	BB87846	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87846_Pcan_Bot2011_IGS	T13-07	1	BB87846_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87846	Sample	BB87846	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-07	IGS	45	BB87846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-07	CGTAATCCTCAGTACTGGCTA	147.81479
BB87846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88938	WGS84	BB87846	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87846_Sspa_Oph2007_COI	T13-07	1	BB87846_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87846	Sample	BB87846	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-07	COI	45	BB87846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-07	GAAGGCCTTCCGATGATC	147.81479
BB87846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88938	WGS84	BB87846	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.306	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87846_Upin_Bot2011_COI	T13-07	1	BB87846_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87846	Sample	BB87846	targeted	Present	Sample	Upin_Bot2011_COI	T13-07	COI	45	BB87846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.81479
BB87847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8289	WGS84	BB87847	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87847_Cjap_Sim2018_COI	T13-08	1	BB87847_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87847	Sample	BB87847	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-08	COI	45	BB87847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30228
BB87847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.8289	WGS84	BB87847	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87847_Mgig_Bot2012_COI	T13-08	1	BB87847_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87847	Sample	BB87847	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-08	COI	45	BB87847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.30228
BB87848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82819	WGS84	BB87848	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87848_Cmae_Bot2011_COI	T13-09	1	BB87848_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87848	Sample	BB87848	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-09	COI	45	BB87848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-09	ATGAACAGTCTATCCTCCTTTAG	147.30194
BB87850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87321	WGS84	BB87850	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87850_Cjap_Sim2018_COI	T13-11	1	BB87850_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87850	Sample	BB87850	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-11	COI	45	BB87850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33664
BB87850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87321	WGS84	BB87850	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87850_Mgig_Bot2012_COI	T13-11	1	BB87850_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87850	Sample	BB87850	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-11	COI	45	BB87850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33664
BB87851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88126	WGS84	BB87851	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.714	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87851_Cmae_Bot2011_COI	T13-12	1	BB87851_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87851	Sample	BB87851	targeted	Present	Sample	Cmae_Bot2011_COI	T13-12	COI	45	BB87851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-12	ATGAACAGTCTATCCTCCTTTAG	147.34203
BB87853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.86907	WGS84	BB87853	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.04	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87853_Aamu_Bax2006_COI	T13-14	1	BB87853_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87853	Sample	BB87853	targeted	Present	Sample	Aamu_Bax2006_COI	T13-14	COI	45	BB87853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-14	GCACAACCGGGATCTTTACTTC	147.33633
BB87853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.86907	WGS84	BB87853	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87853_Pcan_Bot2011_IGS	T13-14	1	BB87853_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87853	Sample	BB87853	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-14	IGS	45	BB87853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-14	CGTAATCCTCAGTACTGGCTA	147.33633
BB87853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.86907	WGS84	BB87853	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87853_Sspa_Oph2007_COI	T13-14	1	BB87853_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87853	Sample	BB87853	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-14	COI	45	BB87853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-14	GAAGGCCTTCCGATGATC	147.33633
BB87853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.86907	WGS84	BB87853	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87853_Upin_Bot2011_COI	T13-14	1	BB87853_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87853	Sample	BB87853	targeted	Absent	Sample	Upin_Bot2011_COI	T13-14	COI	45	BB87853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.33633
BB87854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88452	WGS84	BB87854	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87854_Cjap_Sim2018_COI	T13-15	1	BB87854_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87854	Sample	BB87854	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-15	COI	45	BB87854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33658
BB87854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88452	WGS84	BB87854	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87854_Mgig_Bot2012_COI	T13-15	1	BB87854_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87854	Sample	BB87854	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-15	COI	45	BB87854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.33658
BB87855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88312	WGS84	BB87855	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.901	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87855_Cmae_Bot2011_COI	T13-16	1	BB87855_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87855	Sample	BB87855	targeted	Present	Sample	Cmae_Bot2011_COI	T13-16	COI	45	BB87855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-16	ATGAACAGTCTATCCTCCTTTAG	147.33675
BB87859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77317	WGS84	BB87859	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87859_Cmae_Bot2011_COI	S8-13	1	BB87859_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87859	Sample	BB87859	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-13	COI	45	BB87859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-13	ATGAACAGTCTATCCTCCTTTAG	138.48449
BB87860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77362	WGS84	BB87860	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87860_Aamu_Bax2006_COI	S8-12	1	BB87860_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87860	Sample	BB87860	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-12	COI	45	BB87860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-12	GCACAACCGGGATCTTTACTTC	138.48487
BB87860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77362	WGS84	BB87860	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87860_Pcan_Bot2011_IGS	S8-12	1	BB87860_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87860	Sample	BB87860	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-12	IGS	45	BB87860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-12	CGTAATCCTCAGTACTGGCTA	138.48487
BB87860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77362	WGS84	BB87860	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.353	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87860_Sspa_Oph2007_COI	S8-12	1	BB87860_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87860	Sample	BB87860	targeted	Present	Sample	Sspa_Oph2007_COI	S8-12	COI	45	BB87860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-12	GAAGGCCTTCCGATGATC	138.48487
BB87860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77362	WGS84	BB87860	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87860_Upin_Bot2011_COI	S8-12	1	BB87860_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87860	Sample	BB87860	targeted	Absent	Sample	Upin_Bot2011_COI	S8-12	COI	45	BB87860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.48487
BB87861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77196	WGS84	BB87861	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87861_Cjap_Sim2018_COI	S8-11	1	BB87861_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87861	Sample	BB87861	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-11	COI	45	BB87861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48602
BB87861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77196	WGS84	BB87861	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87861_Mgig_Bot2012_COI	S8-11	1	BB87861_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87861	Sample	BB87861	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-11	COI	45	BB87861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.48602
BB87864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78706	WGS84	BB87864	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87864_Aamu_Bax2006_COI	S8-08	1	BB87864_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87864	Sample	BB87864	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-08	COI	45	BB87864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-08	GCACAACCGGGATCTTTACTTC	138.51353
BB87864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78706	WGS84	BB87864	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87864_Pcan_Bot2011_IGS	S8-08	1	BB87864_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87864	Sample	BB87864	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-08	IGS	45	BB87864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-08	CGTAATCCTCAGTACTGGCTA	138.51353
BB87864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78706	WGS84	BB87864	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.479	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87864_Sspa_Oph2007_COI	S8-08	1	BB87864_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87864	Sample	BB87864	targeted	Present	Sample	Sspa_Oph2007_COI	S8-08	COI	45	BB87864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-08	GAAGGCCTTCCGATGATC	138.51353
BB87864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78706	WGS84	BB87864	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87864_Upin_Bot2011_COI	S8-08	1	BB87864_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87864	Sample	BB87864	targeted	Absent	Sample	Upin_Bot2011_COI	S8-08	COI	45	BB87864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51353
BB87865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78822	WGS84	BB87865	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87865_Cjap_Sim2018_COI	S8-07	1	BB87865_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87865	Sample	BB87865	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-07	COI	45	BB87865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51314
BB87865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78822	WGS84	BB87865	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87865_Mgig_Bot2012_COI	S8-07	1	BB87865_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87865	Sample	BB87865	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-07	COI	45	BB87865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51314
BB87866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78942	WGS84	BB87866	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87866_Cmae_Bot2011_COI	S8-06	1	BB87866_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87866	Sample	BB87866	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-06	COI	45	BB87866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-06	ATGAACAGTCTATCCTCCTTTAG	138.51307
BB87868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87868	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87868_Aamu_Bax2006_COI	S8-17	1	BB87868_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87868	Sample	BB87868	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-17	COI	45	BB87868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-17	GCACAACCGGGATCTTTACTTC	138.49707
BB87868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87868	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87868_Pcan_Bot2011_IGS	S8-17	1	BB87868_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87868	Sample	BB87868	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-17	IGS	45	BB87868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-17	CGTAATCCTCAGTACTGGCTA	138.49707
BB87868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87868	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.333	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87868_Sspa_Oph2007_COI	S8-17	1	BB87868_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87868	Sample	BB87868	targeted	Present	Sample	Sspa_Oph2007_COI	S8-17	COI	45	BB87868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-17	GAAGGCCTTCCGATGATC	138.49707
BB87868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87868	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87868_Upin_Bot2011_COI	S8-17	1	BB87868_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87868	Sample	BB87868	targeted	Absent	Sample	Upin_Bot2011_COI	S8-17	COI	45	BB87868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.49707
BB87869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80964	WGS84	BB87869	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87869_Cjap_Sim2018_COI	S8-03	1	BB87869_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87869	Sample	BB87869	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-03	COI	45	BB87869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52021
BB87869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.80964	WGS84	BB87869	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87869_Mgig_Bot2012_COI	S8-03	1	BB87869_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87869	Sample	BB87869	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-03	COI	45	BB87869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.52021
BB87871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81008	WGS84	BB87871	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87871_Aamu_Bax2006_COI	S8-01	1	BB87871_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87871	Sample	BB87871	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-01	COI	45	BB87871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-01	GCACAACCGGGATCTTTACTTC	138.51996
BB87871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81008	WGS84	BB87871	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87871_Pcan_Bot2011_IGS	S8-01	1	BB87871_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87871	Sample	BB87871	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-01	IGS	45	BB87871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-01	CGTAATCCTCAGTACTGGCTA	138.51996
BB87871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81008	WGS84	BB87871	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.041	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87871_Sspa_Oph2007_COI	S8-01	1	BB87871_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87871	Sample	BB87871	targeted	Present	Sample	Sspa_Oph2007_COI	S8-01	COI	45	BB87871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-01	GAAGGCCTTCCGATGATC	138.51996
BB87871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81008	WGS84	BB87871	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87871_Upin_Bot2011_COI	S8-01	1	BB87871_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87871	Sample	BB87871	targeted	Absent	Sample	Upin_Bot2011_COI	S8-01	COI	45	BB87871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.51996
BB87872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81034	WGS84	BB87872	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87872_Cjap_Sim2018_COI	S6-25	1	BB87872_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87872	Sample	BB87872	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-25	COI	45	BB87872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-25	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52098
BB87872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.81034	WGS84	BB87872	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87872_Mgig_Bot2012_COI	S6-25	1	BB87872_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87872	Sample	BB87872	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-25	COI	45	BB87872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-25	TCTTATTCGTTGGAGACTTTATAACCCT	138.52098
BB87875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8111	WGS84	BB87875	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87875_Aamu_Bax2006_COI	S6-22	1	BB87875_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87875	Sample	BB87875	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-22	COI	45	BB87875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-22	GCACAACCGGGATCTTTACTTC	138.52346
BB87875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8111	WGS84	BB87875	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87875_Pcan_Bot2011_IGS	S6-22	1	BB87875_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87875	Sample	BB87875	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-22	IGS	45	BB87875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-22	CGTAATCCTCAGTACTGGCTA	138.52346
BB87875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8111	WGS84	BB87875	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87875_Sspa_Oph2007_COI	S6-22	1	BB87875_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87875	Sample	BB87875	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-22	COI	45	BB87875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-22	GAAGGCCTTCCGATGATC	138.52346
BB87875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8111	WGS84	BB87875	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87875_Upin_Bot2011_COI	S6-22	1	BB87875_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87875	Sample	BB87875	targeted	Absent	Sample	Upin_Bot2011_COI	S6-22	COI	45	BB87875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-22	CTTTAATTACAGCGTTTTTATTGTTGT	138.52346
BB87876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81155	WGS84	BB87876	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87876_Cjap_Sim2018_COI	S6-21	1	BB87876_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87876	Sample	BB87876	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-21	COI	45	BB87876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-21	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52436
BB87876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.81155	WGS84	BB87876	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.076	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87876_Mgig_Bot2012_COI	S6-21	1	BB87876_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87876	Sample	BB87876	targeted	Present	Sample	Mgig_Bot2012_COI	S6-21	COI	45	BB87876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-21	TCTTATTCGTTGGAGACTTTATAACCCT	138.52436
BB87879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77353	WGS84	BB87879	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87879_Aamu_Bax2006_COI	S6-18	1	BB87879_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87879	Sample	BB87879	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-18	COI	45	BB87879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-18	GCACAACCGGGATCTTTACTTC	138.48586
BB87879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77353	WGS84	BB87879	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87879_Pcan_Bot2011_IGS	S6-18	1	BB87879_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87879	Sample	BB87879	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-18	IGS	45	BB87879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-18	CGTAATCCTCAGTACTGGCTA	138.48586
BB87879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77353	WGS84	BB87879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87879_Sspa_Oph2007_COI	S6-18	1	BB87879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87879	Sample	BB87879	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-18	COI	45	BB87879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-18	GAAGGCCTTCCGATGATC	138.48586
BB87879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77353	WGS84	BB87879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87879_Upin_Bot2011_COI	S6-18	1	BB87879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87879	Sample	BB87879	targeted	Absent	Sample	Upin_Bot2011_COI	S6-18	COI	45	BB87879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.48586
BB87880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77382	WGS84	BB87880	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87880_Cmae_Bot2011_COI	S6-17	1	BB87880_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87880	Sample	BB87880	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-17	COI	45	BB87880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-17	ATGAACAGTCTATCCTCCTTTAG	138.48565
BB87884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78507	WGS84	BB87884	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87884_Cmae_Bot2011_COI	S6-13	1	BB87884_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87884	Sample	BB87884	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-13	COI	45	BB87884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-13	ATGAACAGTCTATCCTCCTTTAG	138.51573
BB87886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78716	WGS84	BB87886	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87886_Aamu_Bax2006_COI	S6-11	1	BB87886_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87886	Sample	BB87886	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-11	COI	45	BB87886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-11	GCACAACCGGGATCTTTACTTC	138.51506
BB87886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78716	WGS84	BB87886	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87886_Pcan_Bot2011_IGS	S6-11	1	BB87886_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87886	Sample	BB87886	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-11	IGS	45	BB87886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-11	CGTAATCCTCAGTACTGGCTA	138.51506
BB87886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78716	WGS84	BB87886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87886_Sspa_Oph2007_COI	S6-11	1	BB87886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87886	Sample	BB87886	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-11	COI	45	BB87886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-11	GAAGGCCTTCCGATGATC	138.51506
BB87886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78716	WGS84	BB87886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87886_Upin_Bot2011_COI	S6-11	1	BB87886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87886	Sample	BB87886	targeted	Absent	Sample	Upin_Bot2011_COI	S6-11	COI	45	BB87886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.51506
BB87890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83688	WGS84	BB87890	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87890_Cjap_Sim2018_COI	S6-07	1	BB87890_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87890	Sample	BB87890	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-07	COI	45	BB87890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50928
BB87890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83688	WGS84	BB87890	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87890_Mgig_Bot2012_COI	S6-07	1	BB87890_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87890	Sample	BB87890	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-07	COI	45	BB87890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.50928
BB87891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83658	WGS84	BB87891	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87891_Cmae_Bot2011_COI	S6-06	1	BB87891_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87891	Sample	BB87891	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-06	COI	45	BB87891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-06	ATGAACAGTCTATCCTCCTTTAG	138.50805
BB87894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84522	WGS84	BB87894	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87894_Cjap_Sim2018_COI	S6-03	1	BB87894_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87894	Sample	BB87894	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-03	COI	45	BB87894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49694
BB87894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84522	WGS84	BB87894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87894_Mgig_Bot2012_COI	S6-03	1	BB87894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87894	Sample	BB87894	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-03	COI	45	BB87894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.49694
BB87895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84619	WGS84	BB87895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87895_Cmae_Bot2011_COI	S6-02	1	BB87895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87895	Sample	BB87895	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-02	COI	45	BB87895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-02	ATGAACAGTCTATCCTCCTTTAG	138.49699
BB87897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85592	WGS84	BB87897	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87897_Aamu_Bax2006_COI	T10-16	1	BB87897_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87897	Sample	BB87897	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-16	COI	45	BB87897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-16	GCACAACCGGGATCTTTACTTC	147.87241
BB87897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85592	WGS84	BB87897	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87897_Pcan_Bot2011_IGS	T10-16	1	BB87897_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87897	Sample	BB87897	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-16	IGS	45	BB87897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-16	CGTAATCCTCAGTACTGGCTA	147.87241
BB87897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85592	WGS84	BB87897	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87897_Sspa_Oph2007_COI	T10-16	1	BB87897_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87897	Sample	BB87897	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-16	COI	45	BB87897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-16	GAAGGCCTTCCGATGATC	147.87241
BB87897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85592	WGS84	BB87897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87897_Upin_Bot2011_COI	T10-16	1	BB87897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87897	Sample	BB87897	targeted	Absent	Sample	Upin_Bot2011_COI	T10-16	COI	45	BB87897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.87241
BB87898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85085	WGS84	BB87898	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87898_Cjap_Sim2018_COI	T10-15	1	BB87898_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87898	Sample	BB87898	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-15	COI	45	BB87898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87391
BB87898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85085	WGS84	BB87898	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.349	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	29/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87898_Mgig_Bot2012_COI	T10-15	1	BB87898_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87898	Sample	BB87898	targeted	Present	Sample	Mgig_Bot2012_COI	T10-15	COI	45	BB87898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.87391
BB87899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84692	WGS84	BB87899	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87899_Cmae_Bot2011_COI	T10-14	1	BB87899_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87899	Sample	BB87899	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-14	COI	45	BB87899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-14	ATGAACAGTCTATCCTCCTTTAG	147.87696
BB87901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88273	WGS84	BB87901	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.136	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87901_Aamu_Bax2006_COI	T10-12	1	BB87901_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87901	Sample	BB87901	targeted	Present	Sample	Aamu_Bax2006_COI	T10-12	COI	45	BB87901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-12	GCACAACCGGGATCTTTACTTC	147.33619
BB87901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88273	WGS84	BB87901	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87901_Pcan_Bot2011_IGS	T10-12	1	BB87901_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87901	Sample	BB87901	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-12	IGS	45	BB87901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-12	CGTAATCCTCAGTACTGGCTA	147.33619
BB87901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88273	WGS84	BB87901	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87901_Sspa_Oph2007_COI	T10-12	1	BB87901_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87901	Sample	BB87901	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-12	COI	45	BB87901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-12	GAAGGCCTTCCGATGATC	147.33619
BB87901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88273	WGS84	BB87901	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87901_Upin_Bot2011_COI	T10-12	1	BB87901_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87901	Sample	BB87901	targeted	Absent	Sample	Upin_Bot2011_COI	T10-12	COI	45	BB87901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.33619
BB87902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88419	WGS84	BB87902	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87902_Cjap_Sim2018_COI	T10-11	1	BB87902_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87902	Sample	BB87902	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-11	COI	45	BB87902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33632
BB87902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88419	WGS84	BB87902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.25	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87902_Mgig_Bot2012_COI	T10-11	1	BB87902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87902	Sample	BB87902	targeted	Present	Sample	Mgig_Bot2012_COI	T10-11	COI	45	BB87902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33632
BB87903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87903_Cmae_Bot2011_COI	T10-10	1	BB87903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87903	Sample	BB87903	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-10	COI	45	BB87903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-10	ATGAACAGTCTATCCTCCTTTAG	147.33542
BB87905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88078	WGS84	BB87905	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87905_Aamu_Bax2006_COI	T10-08	1	BB87905_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87905	Sample	BB87905	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-08	COI	45	BB87905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-08	GCACAACCGGGATCTTTACTTC	147.34172
BB87905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88078	WGS84	BB87905	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87905_Pcan_Bot2011_IGS	T10-08	1	BB87905_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87905	Sample	BB87905	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-08	IGS	45	BB87905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-08	CGTAATCCTCAGTACTGGCTA	147.34172
BB87905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88078	WGS84	BB87905	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87905_Sspa_Oph2007_COI	T10-08	1	BB87905_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87905	Sample	BB87905	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-08	COI	45	BB87905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-08	GAAGGCCTTCCGATGATC	147.34172
BB87905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88078	WGS84	BB87905	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87905_Upin_Bot2011_COI	T10-08	1	BB87905_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87905	Sample	BB87905	targeted	Absent	Sample	Upin_Bot2011_COI	T10-08	COI	45	BB87905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.34172
BB87906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8729	WGS84	BB87906	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87906_Cjap_Sim2018_COI	T10-07	1	BB87906_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87906	Sample	BB87906	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-07	COI	45	BB87906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3363
BB87906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.8729	WGS84	BB87906	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.564	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87906_Mgig_Bot2012_COI	T10-07	1	BB87906_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87906	Sample	BB87906	targeted	Present	Sample	Mgig_Bot2012_COI	T10-07	COI	45	BB87906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.3363
BB87907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82895	WGS84	BB87907	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.161	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87907_Cmae_Bot2011_COI	T10-06	1	BB87907_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87907	Sample	BB87907	targeted	Present	Sample	Cmae_Bot2011_COI	T10-06	COI	45	BB87907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-06	ATGAACAGTCTATCCTCCTTTAG	147.30205
BB87909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82538	WGS84	BB87909	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87909_Aamu_Bax2006_COI	T10-04	1	BB87909_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87909	Sample	BB87909	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-04	COI	45	BB87909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-04	GCACAACCGGGATCTTTACTTC	147.30734
BB87909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82538	WGS84	BB87909	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87909_Pcan_Bot2011_IGS	T10-04	1	BB87909_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87909	Sample	BB87909	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-04	IGS	45	BB87909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-04	CGTAATCCTCAGTACTGGCTA	147.30734
BB87909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82538	WGS84	BB87909	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87909_Sspa_Oph2007_COI	T10-04	1	BB87909_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87909	Sample	BB87909	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-04	COI	45	BB87909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-04	GAAGGCCTTCCGATGATC	147.30734
BB87909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82538	WGS84	BB87909	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87909_Upin_Bot2011_COI	T10-04	1	BB87909_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87909	Sample	BB87909	targeted	Absent	Sample	Upin_Bot2011_COI	T10-04	COI	45	BB87909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.30734
BB87910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96186	WGS84	BB87910	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87910_Cmae_Bot2011_COI	T10-03	1	BB87910_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87910	Sample	BB87910	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-03	COI	45	BB87910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-03	ATGAACAGTCTATCCTCCTTTAG	147.51957
BB87912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96295	WGS84	BB87912	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.715	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87912_Aamu_Bax2006_COI	T10-01	1	BB87912_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87912	Sample	BB87912	targeted	Present	Sample	Aamu_Bax2006_COI	T10-01	COI	45	BB87912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-01	GCACAACCGGGATCTTTACTTC	147.53907
BB87912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96295	WGS84	BB87912	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87912_Pcan_Bot2011_IGS	T10-01	1	BB87912_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87912	Sample	BB87912	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-01	IGS	45	BB87912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-01	CGTAATCCTCAGTACTGGCTA	147.53907
BB87912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96295	WGS84	BB87912	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87912_Sspa_Oph2007_COI	T10-01	1	BB87912_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87912	Sample	BB87912	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-01	COI	45	BB87912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-01	GAAGGCCTTCCGATGATC	147.53907
BB87912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96295	WGS84	BB87912	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87912_Upin_Bot2011_COI	T10-01	1	BB87912_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87912	Sample	BB87912	targeted	Absent	Sample	Upin_Bot2011_COI	T10-01	COI	45	BB87912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.53907
BB87916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81005	WGS84	BB87916	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87916_Aamu_Bax2006_COI	S5-21	1	BB87916_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87916	Sample	BB87916	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-21	COI	45	BB87916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-21	GCACAACCGGGATCTTTACTTC	138.51897
BB87916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81005	WGS84	BB87916	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87916_Pcan_Bot2011_IGS	S5-21	1	BB87916_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87916	Sample	BB87916	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-21	IGS	45	BB87916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-21	CGTAATCCTCAGTACTGGCTA	138.51897
BB87916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81005	WGS84	BB87916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.331	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87916_Sspa_Oph2007_COI	S5-21	1	BB87916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87916	Sample	BB87916	targeted	Present	Sample	Sspa_Oph2007_COI	S5-21	COI	45	BB87916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-21	GAAGGCCTTCCGATGATC	138.51897
BB87916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81005	WGS84	BB87916	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87916_Upin_Bot2011_COI	S5-21	1	BB87916_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87916	Sample	BB87916	targeted	Absent	Sample	Upin_Bot2011_COI	S5-21	COI	45	BB87916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-21	CTTTAATTACAGCGTTTTTATTGTTGT	138.51897
BB87920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77247	WGS84	BB87920	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87920_Cjap_Sim2018_COI	S5-17	1	BB87920_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87920	Sample	BB87920	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-17	COI	45	BB87920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4855
BB87920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77247	WGS84	BB87920	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87920_Mgig_Bot2012_COI	S5-17	1	BB87920_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87920	Sample	BB87920	targeted	Absent	Sample	Mgig_Bot2012_COI	S5-17	COI	45	BB87920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.4855
BB87921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77137	WGS84	BB87921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87921_Cmae_Bot2011_COI	S5-16	1	BB87921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87921	Sample	BB87921	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-16	COI	45	BB87921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-16	ATGAACAGTCTATCCTCCTTTAG	138.48612
BB87924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78534	WGS84	BB87924	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87924_Cjap_Sim2018_COI	S5-13	1	BB87924_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87924	Sample	BB87924	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-13	COI	45	BB87924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51572
BB87924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78534	WGS84	BB87924	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.453	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87924_Mgig_Bot2012_COI	S5-13	1	BB87924_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87924	Sample	BB87924	targeted	Present	Sample	Mgig_Bot2012_COI	S5-13	COI	45	BB87924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.51572
BB87925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78656	WGS84	BB87925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87925_Cmae_Bot2011_COI	S5-12	1	BB87925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87925	Sample	BB87925	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-12	COI	45	BB87925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-12	ATGAACAGTCTATCCTCCTTTAG	138.51548
BB87929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83656	WGS84	BB87929	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87929_Cmae_Bot2011_COI	S5-08	1	BB87929_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87929	Sample	BB87929	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-08	COI	45	BB87929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-08	ATGAACAGTCTATCCTCCTTTAG	138.50972
BB87930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83622	WGS84	BB87930	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87930_Aamu_Bax2006_COI	S5-07	1	BB87930_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87930	Sample	BB87930	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-07	COI	45	BB87930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-07	GCACAACCGGGATCTTTACTTC	138.50894
BB87930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83622	WGS84	BB87930	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87930_Pcan_Bot2011_IGS	S5-07	1	BB87930_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87930	Sample	BB87930	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-07	IGS	45	BB87930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-07	CGTAATCCTCAGTACTGGCTA	138.50894
BB87930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83622	WGS84	BB87930	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.19	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87930_Sspa_Oph2007_COI	S5-07	1	BB87930_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87930	Sample	BB87930	targeted	Present	Sample	Sspa_Oph2007_COI	S5-07	COI	45	BB87930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-07	GAAGGCCTTCCGATGATC	138.50894
BB87930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83622	WGS84	BB87930	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87930_Upin_Bot2011_COI	S5-07	1	BB87930_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87930	Sample	BB87930	targeted	Absent	Sample	Upin_Bot2011_COI	S5-07	COI	45	BB87930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.50894
BB87931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87931	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87931_Cjap_Sim2018_COI	S5-06	1	BB87931_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87931	Sample	BB87931	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-06	COI	45	BB87931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50759
BB87931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83703	WGS84	BB87931	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.828	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87931_Mgig_Bot2012_COI	S5-06	1	BB87931_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87931	Sample	BB87931	targeted	Present	Sample	Mgig_Bot2012_COI	S5-06	COI	45	BB87931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.50759
BB87934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8452	WGS84	BB87934	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87934_Aamu_Bax2006_COI	S5-03	1	BB87934_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87934	Sample	BB87934	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-03	COI	45	BB87934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-03	GCACAACCGGGATCTTTACTTC	138.49715
BB87934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8452	WGS84	BB87934	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87934_Pcan_Bot2011_IGS	S5-03	1	BB87934_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87934	Sample	BB87934	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-03	IGS	45	BB87934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-03	CGTAATCCTCAGTACTGGCTA	138.49715
BB87934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8452	WGS84	BB87934	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87934_Sspa_Oph2007_COI	S5-03	1	BB87934_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87934	Sample	BB87934	targeted	Absent	Sample	Sspa_Oph2007_COI	S5-03	COI	45	BB87934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-03	GAAGGCCTTCCGATGATC	138.49715
BB87934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8452	WGS84	BB87934	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87934_Upin_Bot2011_COI	S5-03	1	BB87934_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87934	Sample	BB87934	targeted	Absent	Sample	Upin_Bot2011_COI	S5-03	COI	45	BB87934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.49715
BB87935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84612	WGS84	BB87935	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87935_Cjap_Sim2018_COI	S5-02	1	BB87935_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87935	Sample	BB87935	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-02	COI	45	BB87935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49706
BB87935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84612	WGS84	BB87935	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.574	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87935_Mgig_Bot2012_COI	S5-02	1	BB87935_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87935	Sample	BB87935	targeted	Present	Sample	Mgig_Bot2012_COI	S5-02	COI	45	BB87935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.49706
BB87936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87936	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87936_Cmae_Bot2011_COI	S5-01	1	BB87936_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87936	Sample	BB87936	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-01	COI	45	BB87936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-01	ATGAACAGTCTATCCTCCTTTAG	138.49736
BB87938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88434	WGS84	BB87938	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87938_Aamu_Bax2006_COI	T9-15	1	BB87938_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87938	Sample	BB87938	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-15	COI	45	BB87938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-15	GCACAACCGGGATCTTTACTTC	147.33624
BB87938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88434	WGS84	BB87938	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87938_Pcan_Bot2011_IGS	T9-15	1	BB87938_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87938	Sample	BB87938	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-15	IGS	45	BB87938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-15	CGTAATCCTCAGTACTGGCTA	147.33624
BB87938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88434	WGS84	BB87938	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87938_Sspa_Oph2007_COI	T9-15	1	BB87938_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87938	Sample	BB87938	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-15	COI	45	BB87938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-15	GAAGGCCTTCCGATGATC	147.33624
BB87938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88434	WGS84	BB87938	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87938_Upin_Bot2011_COI	T9-15	1	BB87938_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87938	Sample	BB87938	targeted	Absent	Sample	Upin_Bot2011_COI	T9-15	COI	45	BB87938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.33624
BB87939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88567	WGS84	BB87939	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87939_Cjap_Sim2018_COI	T9-14	1	BB87939_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87939	Sample	BB87939	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-14	COI	45	BB87939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33529
BB87939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88567	WGS84	BB87939	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.227	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87939_Mgig_Bot2012_COI	T9-14	1	BB87939_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87939	Sample	BB87939	targeted	Present	Sample	Mgig_Bot2012_COI	T9-14	COI	45	BB87939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.33529
BB87941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8813	WGS84	BB87941	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87941_Aamu_Bax2006_COI	T9-12	1	BB87941_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87941	Sample	BB87941	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-12	COI	45	BB87941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-12	GCACAACCGGGATCTTTACTTC	147.3416
BB87941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8813	WGS84	BB87941	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87941_Pcan_Bot2011_IGS	T9-12	1	BB87941_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87941	Sample	BB87941	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-12	IGS	45	BB87941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-12	CGTAATCCTCAGTACTGGCTA	147.3416
BB87941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8813	WGS84	BB87941	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87941_Sspa_Oph2007_COI	T9-12	1	BB87941_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87941	Sample	BB87941	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-12	COI	45	BB87941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-12	GAAGGCCTTCCGATGATC	147.3416
BB87941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8813	WGS84	BB87941	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87941_Upin_Bot2011_COI	T9-12	1	BB87941_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87941	Sample	BB87941	targeted	Absent	Sample	Upin_Bot2011_COI	T9-12	COI	45	BB87941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.3416
BB87942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87302	WGS84	BB87942	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87942_Cjap_Sim2018_COI	T9-11	1	BB87942_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87942	Sample	BB87942	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-11	COI	45	BB87942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33632
BB87942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87302	WGS84	BB87942	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.7	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87942_Mgig_Bot2012_COI	T9-11	1	BB87942_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87942	Sample	BB87942	targeted	Present	Sample	Mgig_Bot2012_COI	T9-11	COI	45	BB87942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33632
BB87943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82517	WGS84	BB87943	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.33	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87943_Cmae_Bot2011_COI	T9-10	1	BB87943_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87943	Sample	BB87943	targeted	Present	Sample	Cmae_Bot2011_COI	T9-10	COI	45	BB87943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-10	ATGAACAGTCTATCCTCCTTTAG	147.30705
BB87945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8292	WGS84	BB87945	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87945_Aamu_Bax2006_COI	T9-08	1	BB87945_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87945	Sample	BB87945	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-08	COI	45	BB87945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-08	GCACAACCGGGATCTTTACTTC	147.30183
BB87945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8292	WGS84	BB87945	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87945_Pcan_Bot2011_IGS	T9-08	1	BB87945_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87945	Sample	BB87945	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-08	IGS	45	BB87945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-08	CGTAATCCTCAGTACTGGCTA	147.30183
BB87945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8292	WGS84	BB87945	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87945_Sspa_Oph2007_COI	T9-08	1	BB87945_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87945	Sample	BB87945	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-08	COI	45	BB87945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-08	GAAGGCCTTCCGATGATC	147.30183
BB87945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8292	WGS84	BB87945	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87945_Upin_Bot2011_COI	T9-08	1	BB87945_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87945	Sample	BB87945	targeted	Absent	Sample	Upin_Bot2011_COI	T9-08	COI	45	BB87945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.30183
BB87946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85585	WGS84	BB87946	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87946_Cjap_Sim2018_COI	T9-07	1	BB87946_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87946	Sample	BB87946	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-07	COI	45	BB87946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87239
BB87946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85585	WGS84	BB87946	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.779	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	23/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87946_Mgig_Bot2012_COI	T9-07	1	BB87946_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87946	Sample	BB87946	targeted	Present	Sample	Mgig_Bot2012_COI	T9-07	COI	45	BB87946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.87239
BB87947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85078	WGS84	BB87947	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87947_Cmae_Bot2011_COI	T9-06	1	BB87947_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87947	Sample	BB87947	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-06	COI	45	BB87947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-06	ATGAACAGTCTATCCTCCTTTAG	147.87365
BB87949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88957	WGS84	BB87949	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.237	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87949_Aamu_Bax2006_COI	T9-04	1	BB87949_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87949	Sample	BB87949	targeted	Present	Sample	Aamu_Bax2006_COI	T9-04	COI	45	BB87949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-04	GCACAACCGGGATCTTTACTTC	147.81295
BB87949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88957	WGS84	BB87949	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87949_Pcan_Bot2011_IGS	T9-04	1	BB87949_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87949	Sample	BB87949	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-04	IGS	45	BB87949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-04	CGTAATCCTCAGTACTGGCTA	147.81295
BB87949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88957	WGS84	BB87949	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87949_Sspa_Oph2007_COI	T9-04	1	BB87949_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87949	Sample	BB87949	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-04	COI	45	BB87949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-04	GAAGGCCTTCCGATGATC	147.81295
BB87949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88957	WGS84	BB87949	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87949_Upin_Bot2011_COI	T9-04	1	BB87949_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87949	Sample	BB87949	targeted	Absent	Sample	Upin_Bot2011_COI	T9-04	COI	45	BB87949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.81295
BB87950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96213	WGS84	BB87950	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87950_Cmae_Bot2011_COI	T9-03	1	BB87950_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87950	Sample	BB87950	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-03	COI	45	BB87950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-03	ATGAACAGTCTATCCTCCTTTAG	147.51947
BB87952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96322	WGS84	BB87952	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.242	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87952_Aamu_Bax2006_COI	T9-01	1	BB87952_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87952	Sample	BB87952	targeted	Present	Sample	Aamu_Bax2006_COI	T9-01	COI	45	BB87952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-01	GCACAACCGGGATCTTTACTTC	147.5383
BB87952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96322	WGS84	BB87952	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87952_Pcan_Bot2011_IGS	T9-01	1	BB87952_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87952	Sample	BB87952	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-01	IGS	45	BB87952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-01	CGTAATCCTCAGTACTGGCTA	147.5383
BB87952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96322	WGS84	BB87952	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87952_Sspa_Oph2007_COI	T9-01	1	BB87952_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87952	Sample	BB87952	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-01	COI	45	BB87952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-01	GAAGGCCTTCCGATGATC	147.5383
BB87952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96322	WGS84	BB87952	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87952_Upin_Bot2011_COI	T9-01	1	BB87952_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87952	Sample	BB87952	targeted	Absent	Sample	Upin_Bot2011_COI	T9-01	COI	45	BB87952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.5383
BB87953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.81114	WGS84	BB87953	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.649	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87953_Mgig_Bot2012_COI	S4-20	1	BB87953_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87953	Sample	BB87953	targeted	Present	Sample	Mgig_Bot2012_COI	S4-20	COI	45	BB87953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-20	TCTTATTCGTTGGAGACTTTATAACCCT	138.52272
BB87956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80946	WGS84	BB87956	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87956_Aamu_Bax2006_COI	S4-17	1	BB87956_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87956	Sample	BB87956	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-17	COI	45	BB87956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-17	GCACAACCGGGATCTTTACTTC	138.51847
BB87956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80946	WGS84	BB87956	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87956_Pcan_Bot2011_IGS	S4-17	1	BB87956_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87956	Sample	BB87956	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-17	IGS	45	BB87956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-17	CGTAATCCTCAGTACTGGCTA	138.51847
BB87956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80946	WGS84	BB87956	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.406	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87956_Sspa_Oph2007_COI	S4-17	1	BB87956_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87956	Sample	BB87956	targeted	Present	Sample	Sspa_Oph2007_COI	S4-17	COI	45	BB87956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-17	GAAGGCCTTCCGATGATC	138.51847
BB87956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80946	WGS84	BB87956	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87956_Upin_Bot2011_COI	S4-17	1	BB87956_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87956	Sample	BB87956	targeted	Absent	Sample	Upin_Bot2011_COI	S4-17	COI	45	BB87956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.51847
BB87957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80913	WGS84	BB87957	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87957_Cjap_Sim2018_COI	S4-16	1	BB87957_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87957	Sample	BB87957	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-16	COI	45	BB87957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51747
BB87957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.80913	WGS84	BB87957	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.301	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87957_Mgig_Bot2012_COI	S4-16	1	BB87957_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87957	Sample	BB87957	targeted	Present	Sample	Mgig_Bot2012_COI	S4-16	COI	45	BB87957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.51747
BB93410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18844	WGS84	BB93410	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93410_Cmae_Bot2011_COI	DD4	1	BB93410_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93410	Sample	BB93410	targeted	Absent	Sample	Cmae_Bot2011_COI	DD4	COI	45	BB93410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD4	ATGAACAGTCTATCCTCCTTTAG	146.36786
BB93410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18844	WGS84	BB93410	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93410_Msal_Bot2012_COI	DD4	1	BB93410_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93410	Sample	BB93410	targeted	Absent	Sample	Msal_Bot2012_COI	DD4	COI	45	BB93410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD4	GYTAGTTCCRATGATGTTAGCTG	146.36786
BB93412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85169	WGS84	BB93412	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93412_Aamu_Bax2006_COI	GD95	1	BB93412_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93412	Sample	BB93412	targeted	Absent	Sample	Aamu_Bax2006_COI	GD95	COI	45	BB93412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD95	GCACAACCGGGATCTTTACTTC	151.31021
BB93412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85169	WGS84	BB93412	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93412_Pcan_Bot2011_IGS	GD95	1	BB93412_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93412	Sample	BB93412	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD95	IGS	45	BB93412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD95	CGTAATCCTCAGTACTGGCTA	151.31021
BB93412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85169	WGS84	BB93412	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93412_Sspa_Oph2007_COI	GD95	1	BB93412_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93412	Sample	BB93412	targeted	Absent	Sample	Sspa_Oph2007_COI	GD95	COI	45	BB93412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD95	GAAGGCCTTCCGATGATC	151.31021
BB93412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85169	WGS84	BB93412	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93412_Upin_Bot2011_COI	GD95	1	BB93412_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93412	Sample	BB93412	targeted	Absent	Sample	Upin_Bot2011_COI	GD95	COI	45	BB93412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD95	CTTTAATTACAGCGTTTTTATTGTTGT	151.31021
BB93413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85318	WGS84	BB93413	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93413_Mgig_Bot2012_COI	GD90	1	BB93413_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93413	Sample	BB93413	targeted	Absent	Sample	Mgig_Bot2012_COI	GD90	COI	45	BB93413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD90	TCTTATTCGTTGGAGACTTTATAACCCT	151.31829
BB93414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7897	WGS84	BB93414	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93414_Cmae_Bot2011_COI	GD80	1	BB93414_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93414	Sample	BB93414	targeted	Absent	Sample	Cmae_Bot2011_COI	GD80	COI	45	BB93414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD80	ATGAACAGTCTATCCTCCTTTAG	151.20943
BB93414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7897	WGS84	BB93414	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93414_Msal_Bot2012_COI	GD80	1	BB93414_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93414	Sample	BB93414	targeted	Absent	Sample	Msal_Bot2012_COI	GD80	COI	45	BB93414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD80	GYTAGTTCCRATGATGTTAGCTG	151.20943
BB93416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78758	WGS84	BB93416	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93416_Aamu_Bax2006_COI	GD65	1	BB93416_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93416	Sample	BB93416	targeted	Absent	Sample	Aamu_Bax2006_COI	GD65	COI	45	BB93416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD65	GCACAACCGGGATCTTTACTTC	151.17433
BB93416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78758	WGS84	BB93416	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93416_Pcan_Bot2011_IGS	GD65	1	BB93416_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93416	Sample	BB93416	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD65	IGS	45	BB93416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD65	CGTAATCCTCAGTACTGGCTA	151.17433
BB93416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78758	WGS84	BB93416	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93416_Sspa_Oph2007_COI	GD65	1	BB93416_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93416	Sample	BB93416	targeted	Absent	Sample	Sspa_Oph2007_COI	GD65	COI	45	BB93416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD65	GAAGGCCTTCCGATGATC	151.17433
BB93416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78758	WGS84	BB93416	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93416_Upin_Bot2011_COI	GD65	1	BB93416_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93416	Sample	BB93416	targeted	Absent	Sample	Upin_Bot2011_COI	GD65	COI	45	BB93416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD65	CTTTAATTACAGCGTTTTTATTGTTGT	151.17433
BB93417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79059	WGS84	BB93417	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93417_Mgig_Bot2012_COI	GD60	1	BB93417_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93417	Sample	BB93417	targeted	Absent	Sample	Mgig_Bot2012_COI	GD60	COI	45	BB93417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD60	TCTTATTCGTTGGAGACTTTATAACCCT	151.17623
BB93418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80527	WGS84	BB93418	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93418_Cmae_Bot2011_COI	GD50	1	BB93418_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93418	Sample	BB93418	targeted	Absent	Sample	Cmae_Bot2011_COI	GD50	COI	45	BB93418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD50	ATGAACAGTCTATCCTCCTTTAG	151.21539
BB93418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80527	WGS84	BB93418	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93418_Msal_Bot2012_COI	GD50	1	BB93418_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93418	Sample	BB93418	targeted	Absent	Sample	Msal_Bot2012_COI	GD50	COI	45	BB93418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD50	GYTAGTTCCRATGATGTTAGCTG	151.21539
BB93420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82257	WGS84	BB93420	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93420_Mgig_Bot2012_COI	GD111	1	BB93420_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93420	Sample	BB93420	targeted	Absent	Sample	Mgig_Bot2012_COI	GD111	COI	45	BB93420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD111	TCTTATTCGTTGGAGACTTTATAACCCT	151.24626
BB93421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83117	WGS84	BB93421	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93421_Cmae_Bot2011_COI	GD34	1	BB93421_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93421	Sample	BB93421	targeted	Absent	Sample	Cmae_Bot2011_COI	GD34	COI	45	BB93421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD34	ATGAACAGTCTATCCTCCTTTAG	151.25505
BB93421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83117	WGS84	BB93421	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93421_Msal_Bot2012_COI	GD34	1	BB93421_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93421	Sample	BB93421	targeted	Absent	Sample	Msal_Bot2012_COI	GD34	COI	45	BB93421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD34	GYTAGTTCCRATGATGTTAGCTG	151.25505
BB93423	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83198	WGS84	BB93423	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93423_Aamu_Bax2006_COI	GD37	1	BB93423_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93423	Sample	BB93423	targeted	Absent	Sample	Aamu_Bax2006_COI	GD37	COI	45	BB93423	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD37	GCACAACCGGGATCTTTACTTC	151.26071
BB93423	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83198	WGS84	BB93423	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93423_Pcan_Bot2011_IGS	GD37	1	BB93423_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93423	Sample	BB93423	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD37	IGS	45	BB93423	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD37	CGTAATCCTCAGTACTGGCTA	151.26071
BB93423	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83198	WGS84	BB93423	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93423_Sspa_Oph2007_COI	GD37	1	BB93423_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93423	Sample	BB93423	targeted	Absent	Sample	Sspa_Oph2007_COI	GD37	COI	45	BB93423	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD37	GAAGGCCTTCCGATGATC	151.26071
BB93423	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83198	WGS84	BB93423	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93423_Upin_Bot2011_COI	GD37	1	BB93423_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93423	Sample	BB93423	targeted	Absent	Sample	Upin_Bot2011_COI	GD37	COI	45	BB93423	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD37	CTTTAATTACAGCGTTTTTATTGTTGT	151.26071
BB93424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8344	WGS84	BB93424	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93424_Mgig_Bot2012_COI	GD39	1	BB93424_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93424	Sample	BB93424	targeted	Absent	Sample	Mgig_Bot2012_COI	GD39	COI	45	BB93424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD39	TCTTATTCGTTGGAGACTTTATAACCCT	151.26551
BB93425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83282	WGS84	BB93425	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93425_Cmae_Bot2011_COI	GD42	1	BB93425_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93425	Sample	BB93425	targeted	Absent	Sample	Cmae_Bot2011_COI	GD42	COI	45	BB93425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD42	ATGAACAGTCTATCCTCCTTTAG	151.26352
BB93425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83282	WGS84	BB93425	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93425_Msal_Bot2012_COI	GD42	1	BB93425_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93425	Sample	BB93425	targeted	Absent	Sample	Msal_Bot2012_COI	GD42	COI	45	BB93425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD42	GYTAGTTCCRATGATGTTAGCTG	151.26352
BB93427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83562	WGS84	BB93427	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93427_Aamu_Bax2006_COI	GD101	1	BB93427_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93427	Sample	BB93427	targeted	Absent	Sample	Aamu_Bax2006_COI	GD101	COI	45	BB93427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD101	GCACAACCGGGATCTTTACTTC	151.27317
BB93427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83562	WGS84	BB93427	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93427_Pcan_Bot2011_IGS	GD101	1	BB93427_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93427	Sample	BB93427	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD101	IGS	45	BB93427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD101	CGTAATCCTCAGTACTGGCTA	151.27317
BB93427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83562	WGS84	BB93427	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93427_Sspa_Oph2007_COI	GD101	1	BB93427_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93427	Sample	BB93427	targeted	Absent	Sample	Sspa_Oph2007_COI	GD101	COI	45	BB93427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD101	GAAGGCCTTCCGATGATC	151.27317
BB93427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83562	WGS84	BB93427	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93427_Upin_Bot2011_COI	GD101	1	BB93427_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93427	Sample	BB93427	targeted	Absent	Sample	Upin_Bot2011_COI	GD101	COI	45	BB93427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD101	CTTTAATTACAGCGTTTTTATTGTTGT	151.27317
BB93428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83454	WGS84	BB93428	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93428_Mgig_Bot2012_COI	GD103	1	BB93428_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93428	Sample	BB93428	targeted	Absent	Sample	Mgig_Bot2012_COI	GD103	COI	45	BB93428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD103	TCTTATTCGTTGGAGACTTTATAACCCT	151.26959
BB93429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80704	WGS84	BB93429	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93429_Cmae_Bot2011_COI	GD47	1	BB93429_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93429	Sample	BB93429	targeted	Absent	Sample	Cmae_Bot2011_COI	GD47	COI	45	BB93429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD47	ATGAACAGTCTATCCTCCTTTAG	151.21656
BB93429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80704	WGS84	BB93429	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93429_Msal_Bot2012_COI	GD47	1	BB93429_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93429	Sample	BB93429	targeted	Absent	Sample	Msal_Bot2012_COI	GD47	COI	45	BB93429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD47	GYTAGTTCCRATGATGTTAGCTG	151.21656
BB93430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80758	WGS84	BB93430	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93430_Aamu_Bax2006_COI	GD48	1	BB93430_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93430	Sample	BB93430	targeted	Absent	Sample	Aamu_Bax2006_COI	GD48	COI	45	BB93430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD48	GCACAACCGGGATCTTTACTTC	151.21655
BB93430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80758	WGS84	BB93430	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93430_Pcan_Bot2011_IGS	GD48	1	BB93430_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93430	Sample	BB93430	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD48	IGS	45	BB93430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD48	CGTAATCCTCAGTACTGGCTA	151.21655
BB93430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80758	WGS84	BB93430	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93430_Sspa_Oph2007_COI	GD48	1	BB93430_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93430	Sample	BB93430	targeted	Absent	Sample	Sspa_Oph2007_COI	GD48	COI	45	BB93430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD48	GAAGGCCTTCCGATGATC	151.21655
BB93430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80758	WGS84	BB93430	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93430_Upin_Bot2011_COI	GD48	1	BB93430_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93430	Sample	BB93430	targeted	Absent	Sample	Upin_Bot2011_COI	GD48	COI	45	BB93430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD48	CTTTAATTACAGCGTTTTTATTGTTGT	151.21655
BB93431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80543	WGS84	BB93431	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93431_Mgig_Bot2012_COI	GD52	1	BB93431_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93431	Sample	BB93431	targeted	Absent	Sample	Mgig_Bot2012_COI	GD52	COI	45	BB93431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD52	TCTTATTCGTTGGAGACTTTATAACCCT	151.21816
BB93432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79141	WGS84	BB93432	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93432_Cmae_Bot2011_COI	GD54	1	BB93432_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93432	Sample	BB93432	targeted	Absent	Sample	Cmae_Bot2011_COI	GD54	COI	45	BB93432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD54	ATGAACAGTCTATCCTCCTTTAG	151.17851
BB93432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79141	WGS84	BB93432	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93432_Msal_Bot2012_COI	GD54	1	BB93432_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93432	Sample	BB93432	targeted	Absent	Sample	Msal_Bot2012_COI	GD54	COI	45	BB93432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD54	GYTAGTTCCRATGATGTTAGCTG	151.17851
BB93434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78964	WGS84	BB93434	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93434_Aamu_Bax2006_COI	GD58	1	BB93434_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93434	Sample	BB93434	targeted	Absent	Sample	Aamu_Bax2006_COI	GD58	COI	45	BB93434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD58	GCACAACCGGGATCTTTACTTC	151.1796
BB93434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78964	WGS84	BB93434	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93434_Pcan_Bot2011_IGS	GD58	1	BB93434_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93434	Sample	BB93434	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD58	IGS	45	BB93434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD58	CGTAATCCTCAGTACTGGCTA	151.1796
BB93434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78964	WGS84	BB93434	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93434_Sspa_Oph2007_COI	GD58	1	BB93434_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93434	Sample	BB93434	targeted	Absent	Sample	Sspa_Oph2007_COI	GD58	COI	45	BB93434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD58	GAAGGCCTTCCGATGATC	151.1796
BB93434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78964	WGS84	BB93434	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93434_Upin_Bot2011_COI	GD58	1	BB93434_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93434	Sample	BB93434	targeted	Absent	Sample	Upin_Bot2011_COI	GD58	COI	45	BB93434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD58	CTTTAATTACAGCGTTTTTATTGTTGT	151.1796
BB93435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78916	WGS84	BB93435	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93435_Mgig_Bot2012_COI	GD61	1	BB93435_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93435	Sample	BB93435	targeted	Absent	Sample	Mgig_Bot2012_COI	GD61	COI	45	BB93435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD61	TCTTATTCGTTGGAGACTTTATAACCCT	151.17678
BB93436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78713	WGS84	BB93436	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93436_Cmae_Bot2011_COI	GD63	1	BB93436_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93436	Sample	BB93436	targeted	Absent	Sample	Cmae_Bot2011_COI	GD63	COI	45	BB93436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD63	ATGAACAGTCTATCCTCCTTTAG	151.17623
BB93436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78713	WGS84	BB93436	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93436_Msal_Bot2012_COI	GD63	1	BB93436_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93436	Sample	BB93436	targeted	Absent	Sample	Msal_Bot2012_COI	GD63	COI	45	BB93436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD63	GYTAGTTCCRATGATGTTAGCTG	151.17623
BB93438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78494	WGS84	BB93438	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93438_Aamu_Bax2006_COI	GD68	1	BB93438_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93438	Sample	BB93438	targeted	Absent	Sample	Aamu_Bax2006_COI	GD68	COI	45	BB93438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD68	GCACAACCGGGATCTTTACTTC	151.17338
BB93438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78494	WGS84	BB93438	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93438_Pcan_Bot2011_IGS	GD68	1	BB93438_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93438	Sample	BB93438	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD68	IGS	45	BB93438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD68	CGTAATCCTCAGTACTGGCTA	151.17338
BB93438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78494	WGS84	BB93438	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93438_Sspa_Oph2007_COI	GD68	1	BB93438_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93438	Sample	BB93438	targeted	Absent	Sample	Sspa_Oph2007_COI	GD68	COI	45	BB93438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD68	GAAGGCCTTCCGATGATC	151.17338
BB93438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78494	WGS84	BB93438	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93438_Upin_Bot2011_COI	GD68	1	BB93438_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93438	Sample	BB93438	targeted	Absent	Sample	Upin_Bot2011_COI	GD68	COI	45	BB93438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD68	CTTTAATTACAGCGTTTTTATTGTTGT	151.17338
BB93439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78618	WGS84	BB93439	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93439_Mgig_Bot2012_COI	GD69	1	BB93439_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93439	Sample	BB93439	targeted	Absent	Sample	Mgig_Bot2012_COI	GD69	COI	45	BB93439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD69	TCTTATTCGTTGGAGACTTTATAACCCT	151.17238
BB93441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.77933	WGS84	BB93441	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93441_Aamu_Bax2006_COI	GD74	1	BB93441_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93441	Sample	BB93441	targeted	Absent	Sample	Aamu_Bax2006_COI	GD74	COI	45	BB93441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD74	GCACAACCGGGATCTTTACTTC	151.19654
BB93441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.77933	WGS84	BB93441	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93441_Pcan_Bot2011_IGS	GD74	1	BB93441_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93441	Sample	BB93441	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD74	IGS	45	BB93441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD74	CGTAATCCTCAGTACTGGCTA	151.19654
BB93441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.77933	WGS84	BB93441	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93441_Sspa_Oph2007_COI	GD74	1	BB93441_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93441	Sample	BB93441	targeted	Absent	Sample	Sspa_Oph2007_COI	GD74	COI	45	BB93441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD74	GAAGGCCTTCCGATGATC	151.19654
BB93441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.77933	WGS84	BB93441	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93441_Upin_Bot2011_COI	GD74	1	BB93441_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93441	Sample	BB93441	targeted	Absent	Sample	Upin_Bot2011_COI	GD74	COI	45	BB93441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD74	CTTTAATTACAGCGTTTTTATTGTTGT	151.19654
BB93442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78083	WGS84	BB93442	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93442_Mgig_Bot2012_COI	GD76	1	BB93442_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93442	Sample	BB93442	targeted	Absent	Sample	Mgig_Bot2012_COI	GD76	COI	45	BB93442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD76	TCTTATTCGTTGGAGACTTTATAACCCT	151.1992
BB93443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78389	WGS84	BB93443	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93443_Cmae_Bot2011_COI	GD78	1	BB93443_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93443	Sample	BB93443	targeted	Absent	Sample	Cmae_Bot2011_COI	GD78	COI	45	BB93443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD78	ATGAACAGTCTATCCTCCTTTAG	151.20134
BB93443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78389	WGS84	BB93443	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93443_Msal_Bot2012_COI	GD78	1	BB93443_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93443	Sample	BB93443	targeted	Absent	Sample	Msal_Bot2012_COI	GD78	COI	45	BB93443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD78	GYTAGTTCCRATGATGTTAGCTG	151.20134
BB93445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79192	WGS84	BB93445	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93445_Aamu_Bax2006_COI	GD82	1	BB93445_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93445	Sample	BB93445	targeted	Absent	Sample	Aamu_Bax2006_COI	GD82	COI	45	BB93445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD82	GCACAACCGGGATCTTTACTTC	151.21144
BB93445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79192	WGS84	BB93445	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93445_Pcan_Bot2011_IGS	GD82	1	BB93445_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93445	Sample	BB93445	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD82	IGS	45	BB93445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD82	CGTAATCCTCAGTACTGGCTA	151.21144
BB93445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79192	WGS84	BB93445	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93445_Sspa_Oph2007_COI	GD82	1	BB93445_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93445	Sample	BB93445	targeted	Absent	Sample	Sspa_Oph2007_COI	GD82	COI	45	BB93445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD82	GAAGGCCTTCCGATGATC	151.21144
BB93445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79192	WGS84	BB93445	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93445_Upin_Bot2011_COI	GD82	1	BB93445_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93445	Sample	BB93445	targeted	Absent	Sample	Upin_Bot2011_COI	GD82	COI	45	BB93445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD82	CTTTAATTACAGCGTTTTTATTGTTGT	151.21144
BB93446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79468	WGS84	BB93446	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93446_Mgig_Bot2012_COI	GD84	1	BB93446_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93446	Sample	BB93446	targeted	Absent	Sample	Mgig_Bot2012_COI	GD84	COI	45	BB93446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD84	TCTTATTCGTTGGAGACTTTATAACCCT	151.21374
BB93447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85551	WGS84	BB93447	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93447_Cmae_Bot2011_COI	GD86	1	BB93447_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93447	Sample	BB93447	targeted	Absent	Sample	Cmae_Bot2011_COI	GD86	COI	45	BB93447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD86	ATGAACAGTCTATCCTCCTTTAG	151.32168
BB93447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85551	WGS84	BB93447	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93447_Msal_Bot2012_COI	GD86	1	BB93447_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93447	Sample	BB93447	targeted	Absent	Sample	Msal_Bot2012_COI	GD86	COI	45	BB93447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD86	GYTAGTTCCRATGATGTTAGCTG	151.32168
BB93449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8526	WGS84	BB93449	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93449_Aamu_Bax2006_COI	GD91	1	BB93449_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93449	Sample	BB93449	targeted	Absent	Sample	Aamu_Bax2006_COI	GD91	COI	45	BB93449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD91	GCACAACCGGGATCTTTACTTC	151.31603
BB93449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8526	WGS84	BB93449	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93449_Pcan_Bot2011_IGS	GD91	1	BB93449_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93449	Sample	BB93449	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD91	IGS	45	BB93449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD91	CGTAATCCTCAGTACTGGCTA	151.31603
BB93449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8526	WGS84	BB93449	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93449_Sspa_Oph2007_COI	GD91	1	BB93449_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93449	Sample	BB93449	targeted	Absent	Sample	Sspa_Oph2007_COI	GD91	COI	45	BB93449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD91	GAAGGCCTTCCGATGATC	151.31603
BB93449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8526	WGS84	BB93449	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93449_Upin_Bot2011_COI	GD91	1	BB93449_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93449	Sample	BB93449	targeted	Absent	Sample	Upin_Bot2011_COI	GD91	COI	45	BB93449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD91	CTTTAATTACAGCGTTTTTATTGTTGT	151.31603
BB93450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85126	WGS84	BB93450	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93450_Cmae_Bot2011_COI	GD93	1	BB93450_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93450	Sample	BB93450	targeted	Absent	Sample	Cmae_Bot2011_COI	GD93	COI	45	BB93450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD93	ATGAACAGTCTATCCTCCTTTAG	151.31393
BB93450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85126	WGS84	BB93450	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93450_Msal_Bot2012_COI	GD93	1	BB93450_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93450	Sample	BB93450	targeted	Absent	Sample	Msal_Bot2012_COI	GD93	COI	45	BB93450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD93	GYTAGTTCCRATGATGTTAGCTG	151.31393
BB93452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81936	WGS84	BB93452	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93452_Aamu_Bax2006_COI	GD10	1	BB93452_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93452	Sample	BB93452	targeted	Absent	Sample	Aamu_Bax2006_COI	GD10	COI	45	BB93452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD10	GCACAACCGGGATCTTTACTTC	151.24067
BB93452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81936	WGS84	BB93452	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93452_Pcan_Bot2011_IGS	GD10	1	BB93452_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93452	Sample	BB93452	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD10	IGS	45	BB93452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD10	CGTAATCCTCAGTACTGGCTA	151.24067
BB93452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81936	WGS84	BB93452	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93452_Sspa_Oph2007_COI	GD10	1	BB93452_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93452	Sample	BB93452	targeted	Absent	Sample	Sspa_Oph2007_COI	GD10	COI	45	BB93452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD10	GAAGGCCTTCCGATGATC	151.24067
BB93452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81936	WGS84	BB93452	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93452_Upin_Bot2011_COI	GD10	1	BB93452_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93452	Sample	BB93452	targeted	Absent	Sample	Upin_Bot2011_COI	GD10	COI	45	BB93452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD10	CTTTAATTACAGCGTTTTTATTGTTGT	151.24067
BB93453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82128	WGS84	BB93453	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93453_Mgig_Bot2012_COI	GD15	1	BB93453_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93453	Sample	BB93453	targeted	Absent	Sample	Mgig_Bot2012_COI	GD15	COI	45	BB93453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD15	TCTTATTCGTTGGAGACTTTATAACCCT	151.24058
BB93454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82217	WGS84	BB93454	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93454_Cmae_Bot2011_COI	GD20	1	BB93454_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93454	Sample	BB93454	targeted	Absent	Sample	Cmae_Bot2011_COI	GD20	COI	45	BB93454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD20	ATGAACAGTCTATCCTCCTTTAG	151.24371
BB93454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82217	WGS84	BB93454	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93454_Msal_Bot2012_COI	GD20	1	BB93454_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93454	Sample	BB93454	targeted	Absent	Sample	Msal_Bot2012_COI	GD20	COI	45	BB93454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD20	GYTAGTTCCRATGATGTTAGCTG	151.24371
BB93456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82442	WGS84	BB93456	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93456_Aamu_Bax2006_COI	GD30	1	BB93456_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93456	Sample	BB93456	targeted	Absent	Sample	Aamu_Bax2006_COI	GD30	COI	45	BB93456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD30	GCACAACCGGGATCTTTACTTC	151.24865
BB93456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82442	WGS84	BB93456	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93456_Pcan_Bot2011_IGS	GD30	1	BB93456_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93456	Sample	BB93456	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD30	IGS	45	BB93456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD30	CGTAATCCTCAGTACTGGCTA	151.24865
BB93456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82442	WGS84	BB93456	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93456_Sspa_Oph2007_COI	GD30	1	BB93456_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93456	Sample	BB93456	targeted	Absent	Sample	Sspa_Oph2007_COI	GD30	COI	45	BB93456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD30	GAAGGCCTTCCGATGATC	151.24865
BB93456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82442	WGS84	BB93456	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93456_Upin_Bot2011_COI	GD30	1	BB93456_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93456	Sample	BB93456	targeted	Absent	Sample	Upin_Bot2011_COI	GD30	COI	45	BB93456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD30	CTTTAATTACAGCGTTTTTATTGTTGT	151.24865
BB93457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82366	WGS84	BB93457	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93457_Mgig_Bot2012_COI	GD105	1	BB93457_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93457	Sample	BB93457	targeted	Absent	Sample	Mgig_Bot2012_COI	GD105	COI	45	BB93457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD105	TCTTATTCGTTGGAGACTTTATAACCCT	151.24798
BB93458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82097	WGS84	BB93458	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93458_Cmae_Bot2011_COI	GD110	1	BB93458_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93458	Sample	BB93458	targeted	Absent	Sample	Cmae_Bot2011_COI	GD110	COI	45	BB93458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD110	ATGAACAGTCTATCCTCCTTTAG	151.24484
BB93458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82097	WGS84	BB93458	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93458_Msal_Bot2012_COI	GD110	1	BB93458_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93458	Sample	BB93458	targeted	Absent	Sample	Msal_Bot2012_COI	GD110	COI	45	BB93458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD110	GYTAGTTCCRATGATGTTAGCTG	151.24484
BB93460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83723	WGS84	BB93460	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93460_Mgig_Bot2012_COI	GD100	1	BB93460_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93460	Sample	BB93460	targeted	Absent	Sample	Mgig_Bot2012_COI	GD100	COI	45	BB93460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD100	TCTTATTCGTTGGAGACTTTATAACCCT	151.27246
BB93461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80734	WGS84	BB93461	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93461_Cmae_Bot2011_COI	GD45	1	BB93461_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93461	Sample	BB93461	targeted	Absent	Sample	Cmae_Bot2011_COI	GD45	COI	45	BB93461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD45	ATGAACAGTCTATCCTCCTTTAG	151.21889
BB93461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80734	WGS84	BB93461	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93461_Msal_Bot2012_COI	GD45	1	BB93461_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93461	Sample	BB93461	targeted	Absent	Sample	Msal_Bot2012_COI	GD45	COI	45	BB93461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD45	GYTAGTTCCRATGATGTTAGCTG	151.21889
BB93463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82693	WGS84	BB93463	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93463_Aamu_Bax2006_COI	GD2	1	BB93463_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93463	Sample	BB93463	targeted	Absent	Sample	Aamu_Bax2006_COI	GD2	COI	45	BB93463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD2	GCACAACCGGGATCTTTACTTC	151.24805
BB93463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82693	WGS84	BB93463	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93463_Pcan_Bot2011_IGS	GD2	1	BB93463_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93463	Sample	BB93463	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD2	IGS	45	BB93463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD2	CGTAATCCTCAGTACTGGCTA	151.24805
BB93463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82693	WGS84	BB93463	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93463_Sspa_Oph2007_COI	GD2	1	BB93463_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93463	Sample	BB93463	targeted	Absent	Sample	Sspa_Oph2007_COI	GD2	COI	45	BB93463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD2	GAAGGCCTTCCGATGATC	151.24805
BB93463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82693	WGS84	BB93463	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93463_Upin_Bot2011_COI	GD2	1	BB93463_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93463	Sample	BB93463	targeted	Absent	Sample	Upin_Bot2011_COI	GD2	COI	45	BB93463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD2	CTTTAATTACAGCGTTTTTATTGTTGT	151.24805
BB93464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81622	WGS84	BB93464	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93464_Mgig_Bot2012_COI	GD3	1	BB93464_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93464	Sample	BB93464	targeted	Absent	Sample	Mgig_Bot2012_COI	GD3	COI	45	BB93464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD3	TCTTATTCGTTGGAGACTTTATAACCCT	151.23706
BB93465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81546	WGS84	BB93465	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93465_Cmae_Bot2011_COI	GD4	1	BB93465_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93465	Sample	BB93465	targeted	Absent	Sample	Cmae_Bot2011_COI	GD4	COI	45	BB93465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD4	ATGAACAGTCTATCCTCCTTTAG	151.23717
BB93465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81546	WGS84	BB93465	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93465_Msal_Bot2012_COI	GD4	1	BB93465_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93465	Sample	BB93465	targeted	Absent	Sample	Msal_Bot2012_COI	GD4	COI	45	BB93465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD4	GYTAGTTCCRATGATGTTAGCTG	151.23717
BB93467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81887	WGS84	BB93467	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93467_Aamu_Bax2006_COI	GD9	1	BB93467_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93467	Sample	BB93467	targeted	Absent	Sample	Aamu_Bax2006_COI	GD9	COI	45	BB93467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD9	GCACAACCGGGATCTTTACTTC	151.23964
BB93467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81887	WGS84	BB93467	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93467_Pcan_Bot2011_IGS	GD9	1	BB93467_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93467	Sample	BB93467	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD9	IGS	45	BB93467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD9	CGTAATCCTCAGTACTGGCTA	151.23964
BB93467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81887	WGS84	BB93467	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93467_Sspa_Oph2007_COI	GD9	1	BB93467_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93467	Sample	BB93467	targeted	Absent	Sample	Sspa_Oph2007_COI	GD9	COI	45	BB93467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD9	GAAGGCCTTCCGATGATC	151.23964
BB93467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81887	WGS84	BB93467	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93467_Upin_Bot2011_COI	GD9	1	BB93467_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93467	Sample	BB93467	targeted	Absent	Sample	Upin_Bot2011_COI	GD9	COI	45	BB93467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD9	CTTTAATTACAGCGTTTTTATTGTTGT	151.23964
BB93468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82046	WGS84	BB93468	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93468_Mgig_Bot2012_COI	GD12	1	BB93468_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93468	Sample	BB93468	targeted	Absent	Sample	Mgig_Bot2012_COI	GD12	COI	45	BB93468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD12	TCTTATTCGTTGGAGACTTTATAACCCT	151.23915
BB93469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82166	WGS84	BB93469	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93469_Cmae_Bot2011_COI	GD14	1	BB93469_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93469	Sample	BB93469	targeted	Absent	Sample	Cmae_Bot2011_COI	GD14	COI	45	BB93469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD14	ATGAACAGTCTATCCTCCTTTAG	151.24024
BB93469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82166	WGS84	BB93469	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93469_Msal_Bot2012_COI	GD14	1	BB93469_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93469	Sample	BB93469	targeted	Absent	Sample	Msal_Bot2012_COI	GD14	COI	45	BB93469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD14	GYTAGTTCCRATGATGTTAGCTG	151.24024
BB93470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81992	WGS84	BB93470	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93470_Aamu_Bax2006_COI	GD17	1	BB93470_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93470	Sample	BB93470	targeted	Absent	Sample	Aamu_Bax2006_COI	GD17	COI	45	BB93470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD17	GCACAACCGGGATCTTTACTTC	151.24107
BB93470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81992	WGS84	BB93470	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93470_Pcan_Bot2011_IGS	GD17	1	BB93470_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93470	Sample	BB93470	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD17	IGS	45	BB93470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD17	CGTAATCCTCAGTACTGGCTA	151.24107
BB93470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81992	WGS84	BB93470	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93470_Sspa_Oph2007_COI	GD17	1	BB93470_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93470	Sample	BB93470	targeted	Absent	Sample	Sspa_Oph2007_COI	GD17	COI	45	BB93470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD17	GAAGGCCTTCCGATGATC	151.24107
BB93470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81992	WGS84	BB93470	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93470_Upin_Bot2011_COI	GD17	1	BB93470_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93470	Sample	BB93470	targeted	Absent	Sample	Upin_Bot2011_COI	GD17	COI	45	BB93470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD17	CTTTAATTACAGCGTTTTTATTGTTGT	151.24107
BB93471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82143	WGS84	BB93471	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93471_Mgig_Bot2012_COI	GD19	1	BB93471_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93471	Sample	BB93471	targeted	Absent	Sample	Mgig_Bot2012_COI	GD19	COI	45	BB93471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD19	TCTTATTCGTTGGAGACTTTATAACCCT	151.24285
BB93472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82256	WGS84	BB93472	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93472_Cmae_Bot2011_COI	GD21	1	BB93472_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93472	Sample	BB93472	targeted	Absent	Sample	Cmae_Bot2011_COI	GD21	COI	45	BB93472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD21	ATGAACAGTCTATCCTCCTTTAG	151.24253
BB93472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82256	WGS84	BB93472	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93472_Msal_Bot2012_COI	GD21	1	BB93472_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93472	Sample	BB93472	targeted	Absent	Sample	Msal_Bot2012_COI	GD21	COI	45	BB93472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD21	GYTAGTTCCRATGATGTTAGCTG	151.24253
BB93474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82492	WGS84	BB93474	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93474_Aamu_Bax2006_COI	GD28	1	BB93474_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93474	Sample	BB93474	targeted	Absent	Sample	Aamu_Bax2006_COI	GD28	COI	45	BB93474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD28	GCACAACCGGGATCTTTACTTC	151.24519
BB93474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82492	WGS84	BB93474	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93474_Pcan_Bot2011_IGS	GD28	1	BB93474_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93474	Sample	BB93474	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD28	IGS	45	BB93474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD28	CGTAATCCTCAGTACTGGCTA	151.24519
BB93474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82492	WGS84	BB93474	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93474_Sspa_Oph2007_COI	GD28	1	BB93474_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93474	Sample	BB93474	targeted	Absent	Sample	Sspa_Oph2007_COI	GD28	COI	45	BB93474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD28	GAAGGCCTTCCGATGATC	151.24519
BB93474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82492	WGS84	BB93474	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93474_Upin_Bot2011_COI	GD28	1	BB93474_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93474	Sample	BB93474	targeted	Absent	Sample	Upin_Bot2011_COI	GD28	COI	45	BB93474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD28	CTTTAATTACAGCGTTTTTATTGTTGT	151.24519
BB93475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82536	WGS84	BB93475	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93475_Mgig_Bot2012_COI	GD32	1	BB93475_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93475	Sample	BB93475	targeted	Absent	Sample	Mgig_Bot2012_COI	GD32	COI	45	BB93475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD32	TCTTATTCGTTGGAGACTTTATAACCCT	151.24636
BB93476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82527	WGS84	BB93476	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93476_Cmae_Bot2011_COI	GD33	1	BB93476_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93476	Sample	BB93476	targeted	Absent	Sample	Cmae_Bot2011_COI	GD33	COI	45	BB93476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD33	ATGAACAGTCTATCCTCCTTTAG	151.24526
BB93476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82527	WGS84	BB93476	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93476_Msal_Bot2012_COI	GD33	1	BB93476_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93476	Sample	BB93476	targeted	Absent	Sample	Msal_Bot2012_COI	GD33	COI	45	BB93476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD33	GYTAGTTCCRATGATGTTAGCTG	151.24526
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.392	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93478_Aamu_Bax2006_COI	MD120	1	BB93478_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Present	Sample	Aamu_Bax2006_COI	MD120	COI	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	GCACAACCGGGATCTTTACTTC	144.90171
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93478_Pcan_Bot2011_IGS	MD120	1	BB93478_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD120	IGS	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	CGTAATCCTCAGTACTGGCTA	144.90171
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.337	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93478_Sspa_Oph2007_COI	MD120	1	BB93478_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Present	Sample	Sspa_Oph2007_COI	MD120	COI	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	GAAGGCCTTCCGATGATC	144.90171
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93478_Upin_Bot2011_COI	MD120	1	BB93478_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Absent	Sample	Upin_Bot2011_COI	MD120	COI	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	CTTTAATTACAGCGTTTTTATTGTTGT	144.90171
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93479_Mgig_Bot2012_COI	MD115	1	BB93479_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Absent	Sample	Mgig_Bot2012_COI	MD115	COI	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	TCTTATTCGTTGGAGACTTTATAACCCT	144.90722
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.478	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93481_Aamu_Bax2006_COI	MD100	1	BB93481_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Present	Sample	Aamu_Bax2006_COI	MD100	COI	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	GCACAACCGGGATCTTTACTTC	144.91348
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93481_Pcan_Bot2011_IGS	MD100	1	BB93481_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD100	IGS	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	CGTAATCCTCAGTACTGGCTA	144.91348
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.338	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93481_Sspa_Oph2007_COI	MD100	1	BB93481_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Present	Sample	Sspa_Oph2007_COI	MD100	COI	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	GAAGGCCTTCCGATGATC	144.91348
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93481_Upin_Bot2011_COI	MD100	1	BB93481_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Absent	Sample	Upin_Bot2011_COI	MD100	COI	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	CTTTAATTACAGCGTTTTTATTGTTGT	144.91348
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93482_Mgig_Bot2012_COI	MD90	1	BB93482_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Absent	Sample	Mgig_Bot2012_COI	MD90	COI	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	TCTTATTCGTTGGAGACTTTATAACCCT	144.91436
BB93483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81802	WGS84	BB93483	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93483_Cmae_Bot2011_COI	MD85	1	BB93483_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93483	Sample	BB93483	targeted	Absent	Sample	Cmae_Bot2011_COI	MD85	COI	45	BB93483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD85	ATGAACAGTCTATCCTCCTTTAG	144.91266
BB93483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81802	WGS84	BB93483	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93483_Msal_Bot2012_COI	MD85	1	BB93483_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93483	Sample	BB93483	targeted	Absent	Sample	Msal_Bot2012_COI	MD85	COI	45	BB93483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD85	GYTAGTTCCRATGATGTTAGCTG	144.91266
BB93485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81669	WGS84	BB93485	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.604	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93485_Aamu_Bax2006_COI	MD70	1	BB93485_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93485	Sample	BB93485	targeted	Present	Sample	Aamu_Bax2006_COI	MD70	COI	45	BB93485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD70	GCACAACCGGGATCTTTACTTC	144.92406
BB93485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81669	WGS84	BB93485	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93485_Pcan_Bot2011_IGS	MD70	1	BB93485_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93485	Sample	BB93485	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD70	IGS	45	BB93485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD70	CGTAATCCTCAGTACTGGCTA	144.92406
BB93485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81669	WGS84	BB93485	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.738	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93485_Sspa_Oph2007_COI	MD70	1	BB93485_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93485	Sample	BB93485	targeted	Present	Sample	Sspa_Oph2007_COI	MD70	COI	45	BB93485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD70	GAAGGCCTTCCGATGATC	144.92406
BB93485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81669	WGS84	BB93485	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93485_Upin_Bot2011_COI	MD70	1	BB93485_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93485	Sample	BB93485	targeted	Absent	Sample	Upin_Bot2011_COI	MD70	COI	45	BB93485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD70	CTTTAATTACAGCGTTTTTATTGTTGT	144.92406
BB93486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81858	WGS84	BB93486	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93486_Mgig_Bot2012_COI	MD65	1	BB93486_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93486	Sample	BB93486	targeted	Absent	Sample	Mgig_Bot2012_COI	MD65	COI	45	BB93486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD65	TCTTATTCGTTGGAGACTTTATAACCCT	144.92668
BB93487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8189	WGS84	BB93487	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93487_Cmae_Bot2011_COI	MD60	1	BB93487_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93487	Sample	BB93487	targeted	Absent	Sample	Cmae_Bot2011_COI	MD60	COI	45	BB93487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD60	ATGAACAGTCTATCCTCCTTTAG	144.92881
BB93487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8189	WGS84	BB93487	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93487_Msal_Bot2012_COI	MD60	1	BB93487_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93487	Sample	BB93487	targeted	Absent	Sample	Msal_Bot2012_COI	MD60	COI	45	BB93487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD60	GYTAGTTCCRATGATGTTAGCTG	144.92881
BB93489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93489	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.811	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93489_Aamu_Bax2006_COI	MD45	1	BB93489_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93489	Sample	BB93489	targeted	Present	Sample	Aamu_Bax2006_COI	MD45	COI	45	BB93489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD45	GCACAACCGGGATCTTTACTTC	144.91313
BB93489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93489	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93489_Pcan_Bot2011_IGS	MD45	1	BB93489_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93489	Sample	BB93489	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD45	IGS	45	BB93489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD45	CGTAATCCTCAGTACTGGCTA	144.91313
BB93489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93489	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.471	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93489_Sspa_Oph2007_COI	MD45	1	BB93489_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93489	Sample	BB93489	targeted	Present	Sample	Sspa_Oph2007_COI	MD45	COI	45	BB93489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD45	GAAGGCCTTCCGATGATC	144.91313
BB93489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93489	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.635	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93489_Upin_Bot2011_COI	MD45	1	BB93489_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93489	Sample	BB93489	targeted	Present	Sample	Upin_Bot2011_COI	MD45	COI	45	BB93489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD45	CTTTAATTACAGCGTTTTTATTGTTGT	144.91313
BB93490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93490	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93490_Cmae_Bot2011_COI	MD35	1	BB93490_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93490	Sample	BB93490	targeted	Absent	Sample	Cmae_Bot2011_COI	MD35	COI	45	BB93490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD35	ATGAACAGTCTATCCTCCTTTAG	144.90751
BB93490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93490	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93490_Msal_Bot2012_COI	MD35	1	BB93490_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93490	Sample	BB93490	targeted	Absent	Sample	Msal_Bot2012_COI	MD35	COI	45	BB93490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD35	GYTAGTTCCRATGATGTTAGCTG	144.90751
BB93492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8467	WGS84	BB93492	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.796	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93492_Aamu_Bax2006_COI	MD20	1	BB93492_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93492	Sample	BB93492	targeted	Present	Sample	Aamu_Bax2006_COI	MD20	COI	45	BB93492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD20	GCACAACCGGGATCTTTACTTC	144.90834
BB93492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8467	WGS84	BB93492	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93492_Pcan_Bot2011_IGS	MD20	1	BB93492_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93492	Sample	BB93492	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD20	IGS	45	BB93492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD20	CGTAATCCTCAGTACTGGCTA	144.90834
BB93492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8467	WGS84	BB93492	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.466	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93492_Sspa_Oph2007_COI	MD20	1	BB93492_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93492	Sample	BB93492	targeted	Present	Sample	Sspa_Oph2007_COI	MD20	COI	45	BB93492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD20	GAAGGCCTTCCGATGATC	144.90834
BB93492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8467	WGS84	BB93492	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.156	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93492_Upin_Bot2011_COI	MD20	1	BB93492_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93492	Sample	BB93492	targeted	Present	Sample	Upin_Bot2011_COI	MD20	COI	45	BB93492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD20	CTTTAATTACAGCGTTTTTATTGTTGT	144.90834
BB93493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82706	WGS84	BB93493	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93493_Mgig_Bot2012_COI	MD124	1	BB93493_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93493	Sample	BB93493	targeted	Absent	Sample	Mgig_Bot2012_COI	MD124	COI	45	BB93493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD124	TCTTATTCGTTGGAGACTTTATAACCCT	144.89997
BB93494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82574	WGS84	BB93494	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93494_Cmae_Bot2011_COI	MD123	1	BB93494_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93494	Sample	BB93494	targeted	Absent	Sample	Cmae_Bot2011_COI	MD123	COI	45	BB93494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD123	ATGAACAGTCTATCCTCCTTTAG	144.90211
BB93494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82574	WGS84	BB93494	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93494_Msal_Bot2012_COI	MD123	1	BB93494_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93494	Sample	BB93494	targeted	Absent	Sample	Msal_Bot2012_COI	MD123	COI	45	BB93494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD123	GYTAGTTCCRATGATGTTAGCTG	144.90211
BB93496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82348	WGS84	BB93496	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.973	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93496_Aamu_Bax2006_COI	MD117	1	BB93496_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93496	Sample	BB93496	targeted	Present	Sample	Aamu_Bax2006_COI	MD117	COI	45	BB93496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD117	GCACAACCGGGATCTTTACTTC	144.90236
BB93496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82348	WGS84	BB93496	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93496_Pcan_Bot2011_IGS	MD117	1	BB93496_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93496	Sample	BB93496	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD117	IGS	45	BB93496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD117	CGTAATCCTCAGTACTGGCTA	144.90236
BB93496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82348	WGS84	BB93496	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.708	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93496_Sspa_Oph2007_COI	MD117	1	BB93496_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93496	Sample	BB93496	targeted	Present	Sample	Sspa_Oph2007_COI	MD117	COI	45	BB93496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD117	GAAGGCCTTCCGATGATC	144.90236
BB93496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82348	WGS84	BB93496	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93496_Upin_Bot2011_COI	MD117	1	BB93496_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93496	Sample	BB93496	targeted	Absent	Sample	Upin_Bot2011_COI	MD117	COI	45	BB93496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD117	CTTTAATTACAGCGTTTTTATTGTTGT	144.90236
BB93497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8209	WGS84	BB93497	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93497_Mgig_Bot2012_COI	MD113	1	BB93497_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93497	Sample	BB93497	targeted	Absent	Sample	Mgig_Bot2012_COI	MD113	COI	45	BB93497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD113	TCTTATTCGTTGGAGACTTTATAACCCT	144.90658
BB93498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81641	WGS84	BB93498	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93498_Cmae_Bot2011_COI	MD111	1	BB93498_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93498	Sample	BB93498	targeted	Absent	Sample	Cmae_Bot2011_COI	MD111	COI	45	BB93498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD111	ATGAACAGTCTATCCTCCTTTAG	144.90597
BB93498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81641	WGS84	BB93498	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93498_Msal_Bot2012_COI	MD111	1	BB93498_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93498	Sample	BB93498	targeted	Absent	Sample	Msal_Bot2012_COI	MD111	COI	45	BB93498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD111	GYTAGTTCCRATGATGTTAGCTG	144.90597
BB93500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82007	WGS84	BB93500	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.347	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93500_Aamu_Bax2006_COI	MD107	1	BB93500_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93500	Sample	BB93500	targeted	Present	Sample	Aamu_Bax2006_COI	MD107	COI	45	BB93500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD107	GCACAACCGGGATCTTTACTTC	144.90792
BB93500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82007	WGS84	BB93500	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93500_Pcan_Bot2011_IGS	MD107	1	BB93500_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93500	Sample	BB93500	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD107	IGS	45	BB93500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD107	CGTAATCCTCAGTACTGGCTA	144.90792
BB93500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82007	WGS84	BB93500	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.314	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93500_Sspa_Oph2007_COI	MD107	1	BB93500_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93500	Sample	BB93500	targeted	Present	Sample	Sspa_Oph2007_COI	MD107	COI	45	BB93500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD107	GAAGGCCTTCCGATGATC	144.90792
BB93500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82007	WGS84	BB93500	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93500_Upin_Bot2011_COI	MD107	1	BB93500_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93500	Sample	BB93500	targeted	Absent	Sample	Upin_Bot2011_COI	MD107	COI	45	BB93500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD107	CTTTAATTACAGCGTTTTTATTGTTGT	144.90792
BB93501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82138	WGS84	BB93501	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93501_Mgig_Bot2012_COI	MD106	1	BB93501_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93501	Sample	BB93501	targeted	Absent	Sample	Mgig_Bot2012_COI	MD106	COI	45	BB93501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD106	TCTTATTCGTTGGAGACTTTATAACCCT	144.90934
BB93502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82112	WGS84	BB93502	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93502_Cmae_Bot2011_COI	MD104	1	BB93502_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93502	Sample	BB93502	targeted	Absent	Sample	Cmae_Bot2011_COI	MD104	COI	45	BB93502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD104	ATGAACAGTCTATCCTCCTTTAG	144.91172
BB93502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82112	WGS84	BB93502	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93502_Msal_Bot2012_COI	MD104	1	BB93502_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93502	Sample	BB93502	targeted	Absent	Sample	Msal_Bot2012_COI	MD104	COI	45	BB93502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD104	GYTAGTTCCRATGATGTTAGCTG	144.91172
BB93504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93504	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.486	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93504_Aamu_Bax2006_COI	MD98	1	BB93504_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93504	Sample	BB93504	targeted	Present	Sample	Aamu_Bax2006_COI	MD98	COI	45	BB93504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD98	GCACAACCGGGATCTTTACTTC	144.91364
BB93504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93504	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93504_Pcan_Bot2011_IGS	MD98	1	BB93504_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93504	Sample	BB93504	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD98	IGS	45	BB93504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD98	CGTAATCCTCAGTACTGGCTA	144.91364
BB93504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93504	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.523	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93504_Sspa_Oph2007_COI	MD98	1	BB93504_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93504	Sample	BB93504	targeted	Present	Sample	Sspa_Oph2007_COI	MD98	COI	45	BB93504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD98	GAAGGCCTTCCGATGATC	144.91364
BB93504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93504	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93504_Upin_Bot2011_COI	MD98	1	BB93504_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93504	Sample	BB93504	targeted	Absent	Sample	Upin_Bot2011_COI	MD98	COI	45	BB93504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD98	CTTTAATTACAGCGTTTTTATTGTTGT	144.91364
BB93505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81014	WGS84	BB93505	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93505_Mgig_Bot2012_COI	MD96	1	BB93505_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93505	Sample	BB93505	targeted	Absent	Sample	Mgig_Bot2012_COI	MD96	COI	45	BB93505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD96	TCTTATTCGTTGGAGACTTTATAACCCT	144.91453
BB93506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81156	WGS84	BB93506	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93506_Cmae_Bot2011_COI	MD94	1	BB93506_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93506	Sample	BB93506	targeted	Absent	Sample	Cmae_Bot2011_COI	MD94	COI	45	BB93506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD94	ATGAACAGTCTATCCTCCTTTAG	144.91409
BB93506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81156	WGS84	BB93506	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93506_Msal_Bot2012_COI	MD94	1	BB93506_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93506	Sample	BB93506	targeted	Absent	Sample	Msal_Bot2012_COI	MD94	COI	45	BB93506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD94	GYTAGTTCCRATGATGTTAGCTG	144.91409
BB93508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81445	WGS84	BB93508	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.914	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93508_Aamu_Bax2006_COI	MD91	1	BB93508_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93508	Sample	BB93508	targeted	Present	Sample	Aamu_Bax2006_COI	MD91	COI	45	BB93508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD91	GCACAACCGGGATCTTTACTTC	144.91369
BB93508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81445	WGS84	BB93508	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93508_Pcan_Bot2011_IGS	MD91	1	BB93508_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93508	Sample	BB93508	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD91	IGS	45	BB93508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD91	CGTAATCCTCAGTACTGGCTA	144.91369
BB93508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81445	WGS84	BB93508	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.245	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93508_Sspa_Oph2007_COI	MD91	1	BB93508_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93508	Sample	BB93508	targeted	Present	Sample	Sspa_Oph2007_COI	MD91	COI	45	BB93508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD91	GAAGGCCTTCCGATGATC	144.91369
BB93508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81445	WGS84	BB93508	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93508_Upin_Bot2011_COI	MD91	1	BB93508_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93508	Sample	BB93508	targeted	Absent	Sample	Upin_Bot2011_COI	MD91	COI	45	BB93508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD91	CTTTAATTACAGCGTTTTTATTGTTGT	144.91369
BB93509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81584	WGS84	BB93509	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93509_Mgig_Bot2012_COI	MD88	1	BB93509_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93509	Sample	BB93509	targeted	Absent	Sample	Mgig_Bot2012_COI	MD88	COI	45	BB93509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD88	TCTTATTCGTTGGAGACTTTATAACCCT	144.91397
BB93511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81836	WGS84	BB93511	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.109	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93511_Aamu_Bax2006_COI	MD83	1	BB93511_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93511	Sample	BB93511	targeted	Present	Sample	Aamu_Bax2006_COI	MD83	COI	45	BB93511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD83	GCACAACCGGGATCTTTACTTC	144.91492
BB93511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81836	WGS84	BB93511	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93511_Pcan_Bot2011_IGS	MD83	1	BB93511_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93511	Sample	BB93511	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD83	IGS	45	BB93511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD83	CGTAATCCTCAGTACTGGCTA	144.91492
BB93511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81836	WGS84	BB93511	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.61	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93511_Sspa_Oph2007_COI	MD83	1	BB93511_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93511	Sample	BB93511	targeted	Present	Sample	Sspa_Oph2007_COI	MD83	COI	45	BB93511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD83	GAAGGCCTTCCGATGATC	144.91492
BB93511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81836	WGS84	BB93511	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93511_Upin_Bot2011_COI	MD83	1	BB93511_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93511	Sample	BB93511	targeted	Absent	Sample	Upin_Bot2011_COI	MD83	COI	45	BB93511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD83	CTTTAATTACAGCGTTTTTATTGTTGT	144.91492
BB93512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81938	WGS84	BB93512	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93512_Mgig_Bot2012_COI	MD82	1	BB93512_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93512	Sample	BB93512	targeted	Absent	Sample	Mgig_Bot2012_COI	MD82	COI	45	BB93512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD82	TCTTATTCGTTGGAGACTTTATAACCCT	144.91696
BB93513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81971	WGS84	BB93513	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93513_Cmae_Bot2011_COI	MD79	1	BB93513_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93513	Sample	BB93513	targeted	Absent	Sample	Cmae_Bot2011_COI	MD79	COI	45	BB93513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD79	ATGAACAGTCTATCCTCCTTTAG	144.91962
BB93513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81971	WGS84	BB93513	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93513_Msal_Bot2012_COI	MD79	1	BB93513_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93513	Sample	BB93513	targeted	Absent	Sample	Msal_Bot2012_COI	MD79	COI	45	BB93513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD79	GYTAGTTCCRATGATGTTAGCTG	144.91962
BB93515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81903	WGS84	BB93515	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.63	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93515_Aamu_Bax2006_COI	MD74	1	BB93515_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93515	Sample	BB93515	targeted	Present	Sample	Aamu_Bax2006_COI	MD74	COI	45	BB93515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD74	GCACAACCGGGATCTTTACTTC	144.92243
BB93515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81903	WGS84	BB93515	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93515_Pcan_Bot2011_IGS	MD74	1	BB93515_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93515	Sample	BB93515	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD74	IGS	45	BB93515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD74	CGTAATCCTCAGTACTGGCTA	144.92243
BB93515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81903	WGS84	BB93515	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.649	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93515_Sspa_Oph2007_COI	MD74	1	BB93515_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93515	Sample	BB93515	targeted	Present	Sample	Sspa_Oph2007_COI	MD74	COI	45	BB93515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD74	GAAGGCCTTCCGATGATC	144.92243
BB93515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81903	WGS84	BB93515	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93515_Upin_Bot2011_COI	MD74	1	BB93515_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93515	Sample	BB93515	targeted	Absent	Sample	Upin_Bot2011_COI	MD74	COI	45	BB93515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD74	CTTTAATTACAGCGTTTTTATTGTTGT	144.92243
BB93516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8197	WGS84	BB93516	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93516_Mgig_Bot2012_COI	MD73	1	BB93516_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93516	Sample	BB93516	targeted	Absent	Sample	Mgig_Bot2012_COI	MD73	COI	45	BB93516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD73	TCTTATTCGTTGGAGACTTTATAACCCT	144.92558
BB93517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81825	WGS84	BB93517	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93517_Cmae_Bot2011_COI	MD71	1	BB93517_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93517	Sample	BB93517	targeted	Absent	Sample	Cmae_Bot2011_COI	MD71	COI	45	BB93517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD71	ATGAACAGTCTATCCTCCTTTAG	144.92325
BB93517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81825	WGS84	BB93517	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93517_Msal_Bot2012_COI	MD71	1	BB93517_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93517	Sample	BB93517	targeted	Absent	Sample	Msal_Bot2012_COI	MD71	COI	45	BB93517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD71	GYTAGTTCCRATGATGTTAGCTG	144.92325
BB93519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8179	WGS84	BB93519	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.26	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93519_Aamu_Bax2006_COI	MD62	1	BB93519_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93519	Sample	BB93519	targeted	Present	Sample	Aamu_Bax2006_COI	MD62	COI	45	BB93519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD62	GCACAACCGGGATCTTTACTTC	144.92486
BB93519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8179	WGS84	BB93519	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93519_Pcan_Bot2011_IGS	MD62	1	BB93519_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93519	Sample	BB93519	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD62	IGS	45	BB93519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD62	CGTAATCCTCAGTACTGGCTA	144.92486
BB93519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8179	WGS84	BB93519	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.575	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93519_Sspa_Oph2007_COI	MD62	1	BB93519_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93519	Sample	BB93519	targeted	Present	Sample	Sspa_Oph2007_COI	MD62	COI	45	BB93519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD62	GAAGGCCTTCCGATGATC	144.92486
BB93519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8179	WGS84	BB93519	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93519_Upin_Bot2011_COI	MD62	1	BB93519_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93519	Sample	BB93519	targeted	Absent	Sample	Upin_Bot2011_COI	MD62	COI	45	BB93519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD62	CTTTAATTACAGCGTTTTTATTGTTGT	144.92486
BB93520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81935	WGS84	BB93520	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93520_Cmae_Bot2011_COI	MD63	1	BB93520_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93520	Sample	BB93520	targeted	Absent	Sample	Cmae_Bot2011_COI	MD63	COI	45	BB93520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD63	ATGAACAGTCTATCCTCCTTTAG	144.92706
BB93520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81935	WGS84	BB93520	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93520_Msal_Bot2012_COI	MD63	1	BB93520_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93520	Sample	BB93520	targeted	Absent	Sample	Msal_Bot2012_COI	MD63	COI	45	BB93520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD63	GYTAGTTCCRATGATGTTAGCTG	144.92706
BB93522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93522	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.664	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93522_Aamu_Bax2006_COI	MD57	1	BB93522_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93522	Sample	BB93522	targeted	Present	Sample	Aamu_Bax2006_COI	MD57	COI	45	BB93522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD57	GCACAACCGGGATCTTTACTTC	144.93141
BB93522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93522	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93522_Pcan_Bot2011_IGS	MD57	1	BB93522_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93522	Sample	BB93522	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD57	IGS	45	BB93522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD57	CGTAATCCTCAGTACTGGCTA	144.93141
BB93522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93522	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.565	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93522_Sspa_Oph2007_COI	MD57	1	BB93522_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93522	Sample	BB93522	targeted	Present	Sample	Sspa_Oph2007_COI	MD57	COI	45	BB93522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD57	GAAGGCCTTCCGATGATC	144.93141
BB93522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93522	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93522_Upin_Bot2011_COI	MD57	1	BB93522_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93522	Sample	BB93522	targeted	Absent	Sample	Upin_Bot2011_COI	MD57	COI	45	BB93522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD57	CTTTAATTACAGCGTTTTTATTGTTGT	144.93141
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93523_Mgig_Bot2012_COI	MD53	1	BB93523_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Absent	Sample	Mgig_Bot2012_COI	MD53	COI	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	TCTTATTCGTTGGAGACTTTATAACCCT	144.91028
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93524_Cmae_Bot2011_COI	MD51	1	BB93524_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Absent	Sample	Cmae_Bot2011_COI	MD51	COI	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	ATGAACAGTCTATCCTCCTTTAG	144.91089
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93524_Hsan_Wil2021_COI	MD51	1	BB93524_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Absent	Sample	Hsan_Wil2021_COI	MD51	COI	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	CCTGGGCCGGTATAGTAGGT	144.91089
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93524_Msal_Bot2012_COI	MD51	1	BB93524_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Absent	Sample	Msal_Bot2012_COI	MD51	COI	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	GYTAGTTCCRATGATGTTAGCTG	144.91089
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.636	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93526_Aamu_Bax2006_COI	MD48	1	BB93526_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Present	Sample	Aamu_Bax2006_COI	MD48	COI	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	GCACAACCGGGATCTTTACTTC	144.91415
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93526_Pcan_Bot2011_IGS	MD48	1	BB93526_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD48	IGS	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	CGTAATCCTCAGTACTGGCTA	144.91415
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.036	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93526_Sspa_Oph2007_COI	MD48	1	BB93526_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Present	Sample	Sspa_Oph2007_COI	MD48	COI	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	GAAGGCCTTCCGATGATC	144.91415
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.6	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93526_Upin_Bot2011_COI	MD48	1	BB93526_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Present	Sample	Upin_Bot2011_COI	MD48	COI	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	CTTTAATTACAGCGTTTTTATTGTTGT	144.91415
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93527_Mgig_Bot2012_COI	MD44	1	BB93527_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Absent	Sample	Mgig_Bot2012_COI	MD44	COI	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	TCTTATTCGTTGGAGACTTTATAACCCT	144.91391
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93528_Cmae_Bot2011_COI	MD43	1	BB93528_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Absent	Sample	Cmae_Bot2011_COI	MD43	COI	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	ATGAACAGTCTATCCTCCTTTAG	144.91599
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93528_Hsan_Wil2021_COI	MD43	1	BB93528_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Absent	Sample	Hsan_Wil2021_COI	MD43	COI	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	CCTGGGCCGGTATAGTAGGT	144.91599
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93528_Msal_Bot2012_COI	MD43	1	BB93528_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Absent	Sample	Msal_Bot2012_COI	MD43	COI	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	GYTAGTTCCRATGATGTTAGCTG	144.91599
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93530_Mgig_Bot2012_COI	MD38	1	BB93530_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Absent	Sample	Mgig_Bot2012_COI	MD38	COI	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	TCTTATTCGTTGGAGACTTTATAACCCT	144.91628
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93531_Cmae_Bot2011_COI	MD37	1	BB93531_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Absent	Sample	Cmae_Bot2011_COI	MD37	COI	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	ATGAACAGTCTATCCTCCTTTAG	144.9044
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93531_Hsan_Wil2021_COI	MD37	1	BB93531_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Absent	Sample	Hsan_Wil2021_COI	MD37	COI	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	CCTGGGCCGGTATAGTAGGT	144.9044
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93531_Msal_Bot2012_COI	MD37	1	BB93531_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Absent	Sample	Msal_Bot2012_COI	MD37	COI	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	GYTAGTTCCRATGATGTTAGCTG	144.9044
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.53	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93533_Aamu_Bax2006_COI	MD33	1	BB93533_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Present	Sample	Aamu_Bax2006_COI	MD33	COI	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	GCACAACCGGGATCTTTACTTC	144.91481
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93533_Pcan_Bot2011_IGS	MD33	1	BB93533_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD33	IGS	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	CGTAATCCTCAGTACTGGCTA	144.91481
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.241	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93533_Sspa_Oph2007_COI	MD33	1	BB93533_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Present	Sample	Sspa_Oph2007_COI	MD33	COI	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	GAAGGCCTTCCGATGATC	144.91481
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.167	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93533_Upin_Bot2011_COI	MD33	1	BB93533_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Present	Sample	Upin_Bot2011_COI	MD33	COI	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	CTTTAATTACAGCGTTTTTATTGTTGT	144.91481
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93534_Mgig_Bot2012_COI	MD28	1	BB93534_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Absent	Sample	Mgig_Bot2012_COI	MD28	COI	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	TCTTATTCGTTGGAGACTTTATAACCCT	144.9158
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.75	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93535_Cmae_Bot2011_COI	MD26	1	BB93535_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Present	Sample	Cmae_Bot2011_COI	MD26	COI	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	ATGAACAGTCTATCCTCCTTTAG	144.91145
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93535_Hsan_Wil2021_COI	MD26	1	BB93535_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Absent	Sample	Hsan_Wil2021_COI	MD26	COI	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	CCTGGGCCGGTATAGTAGGT	144.91145
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93535_Msal_Bot2012_COI	MD26	1	BB93535_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Absent	Sample	Msal_Bot2012_COI	MD26	COI	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	GYTAGTTCCRATGATGTTAGCTG	144.91145
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.375	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93537_Aamu_Bax2006_COI	MD21	1	BB93537_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Present	Sample	Aamu_Bax2006_COI	MD21	COI	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	GCACAACCGGGATCTTTACTTC	144.90981
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93537_Pcan_Bot2011_IGS	MD21	1	BB93537_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD21	IGS	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	CGTAATCCTCAGTACTGGCTA	144.90981
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.691	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93537_Sspa_Oph2007_COI	MD21	1	BB93537_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Present	Sample	Sspa_Oph2007_COI	MD21	COI	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	GAAGGCCTTCCGATGATC	144.90981
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.243	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93537_Upin_Bot2011_COI	MD21	1	BB93537_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Present	Sample	Upin_Bot2011_COI	MD21	COI	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	CTTTAATTACAGCGTTTTTATTGTTGT	144.90981
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93538_Mgig_Bot2012_COI	MD19	1	BB93538_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Absent	Sample	Mgig_Bot2012_COI	MD19	COI	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	TCTTATTCGTTGGAGACTTTATAACCCT	144.90713
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93539_Cmae_Bot2011_COI	MD17	1	BB93539_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Absent	Sample	Cmae_Bot2011_COI	MD17	COI	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	ATGAACAGTCTATCCTCCTTTAG	144.90621
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93539_Hsan_Wil2021_COI	MD17	1	BB93539_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Absent	Sample	Hsan_Wil2021_COI	MD17	COI	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	CCTGGGCCGGTATAGTAGGT	144.90621
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93539_Msal_Bot2012_COI	MD17	1	BB93539_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Absent	Sample	Msal_Bot2012_COI	MD17	COI	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	GYTAGTTCCRATGATGTTAGCTG	144.90621
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.116	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93540_Aamu_Bax2006_COI	MD16	1	BB93540_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Present	Sample	Aamu_Bax2006_COI	MD16	COI	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	GCACAACCGGGATCTTTACTTC	144.90716
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93540_Pcan_Bot2011_IGS	MD16	1	BB93540_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD16	IGS	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	CGTAATCCTCAGTACTGGCTA	144.90716
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.366	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93540_Sspa_Oph2007_COI	MD16	1	BB93540_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Present	Sample	Sspa_Oph2007_COI	MD16	COI	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	GAAGGCCTTCCGATGATC	144.90716
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.363	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93540_Upin_Bot2011_COI	MD16	1	BB93540_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Present	Sample	Upin_Bot2011_COI	MD16	COI	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	CTTTAATTACAGCGTTTTTATTGTTGT	144.90716
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93541_Mgig_Bot2012_COI	MD14	1	BB93541_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Absent	Sample	Mgig_Bot2012_COI	MD14	COI	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	TCTTATTCGTTGGAGACTTTATAACCCT	144.90545
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93542_Cmae_Bot2011_COI	MD12	1	BB93542_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Absent	Sample	Cmae_Bot2011_COI	MD12	COI	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	ATGAACAGTCTATCCTCCTTTAG	144.90411
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93542_Hsan_Wil2021_COI	MD12	1	BB93542_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Absent	Sample	Hsan_Wil2021_COI	MD12	COI	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	CCTGGGCCGGTATAGTAGGT	144.90411
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93542_Msal_Bot2012_COI	MD12	1	BB93542_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Absent	Sample	Msal_Bot2012_COI	MD12	COI	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	GYTAGTTCCRATGATGTTAGCTG	144.90411
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.379	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93544_Aamu_Bax2006_COI	MD9	1	BB93544_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Present	Sample	Aamu_Bax2006_COI	MD9	COI	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	GCACAACCGGGATCTTTACTTC	144.92807
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93544_Pcan_Bot2011_IGS	MD9	1	BB93544_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD9	IGS	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	CGTAATCCTCAGTACTGGCTA	144.92807
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.237	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93544_Sspa_Oph2007_COI	MD9	1	BB93544_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Present	Sample	Sspa_Oph2007_COI	MD9	COI	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	GAAGGCCTTCCGATGATC	144.92807
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	28.707	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93544_Upin_Bot2011_COI	MD9	1	BB93544_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Present	Sample	Upin_Bot2011_COI	MD9	COI	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	CTTTAATTACAGCGTTTTTATTGTTGT	144.92807
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93545_Mgig_Bot2012_COI	MD7	1	BB93545_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Absent	Sample	Mgig_Bot2012_COI	MD7	COI	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	TCTTATTCGTTGGAGACTTTATAACCCT	144.92959
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.582	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93546_Cmae_Bot2011_COI	MD6	1	BB93546_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Present	Sample	Cmae_Bot2011_COI	MD6	COI	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	ATGAACAGTCTATCCTCCTTTAG	144.92993
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93546_Hsan_Wil2021_COI	MD6	1	BB93546_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Absent	Sample	Hsan_Wil2021_COI	MD6	COI	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	CCTGGGCCGGTATAGTAGGT	144.92993
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93546_Msal_Bot2012_COI	MD6	1	BB93546_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Absent	Sample	Msal_Bot2012_COI	MD6	COI	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	GYTAGTTCCRATGATGTTAGCTG	144.92993
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.787	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93548_Aamu_Bax2006_COI	MD3	1	BB93548_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Present	Sample	Aamu_Bax2006_COI	MD3	COI	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	GCACAACCGGGATCTTTACTTC	144.93105
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93548_Pcan_Bot2011_IGS	MD3	1	BB93548_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD3	IGS	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	CGTAATCCTCAGTACTGGCTA	144.93105
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.236	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93548_Sspa_Oph2007_COI	MD3	1	BB93548_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Present	Sample	Sspa_Oph2007_COI	MD3	COI	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	GAAGGCCTTCCGATGATC	144.93105
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93548_Upin_Bot2011_COI	MD3	1	BB93548_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Absent	Sample	Upin_Bot2011_COI	MD3	COI	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	CTTTAATTACAGCGTTTTTATTGTTGT	144.93105
BB93549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36339	WGS84	BB93549	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93549_Mgig_Bot2012_COI	BD60	1	BB93549_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93549	Sample	BB93549	targeted	Absent	Sample	Mgig_Bot2012_COI	BD60	COI	45	BB93549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD60	TCTTATTCGTTGGAGACTTTATAACCCT	153.17593
BB93551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37878	WGS84	BB93551	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93551_Aamu_Bax2006_COI	BD50	1	BB93551_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93551	Sample	BB93551	targeted	Absent	Sample	Aamu_Bax2006_COI	BD50	COI	45	BB93551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD50	GCACAACCGGGATCTTTACTTC	153.1647
BB93551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37878	WGS84	BB93551	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93551_Pcan_Bot2011_IGS	BD50	1	BB93551_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93551	Sample	BB93551	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD50	IGS	45	BB93551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD50	CGTAATCCTCAGTACTGGCTA	153.1647
BB93551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37878	WGS84	BB93551	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93551_Sspa_Oph2007_COI	BD50	1	BB93551_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93551	Sample	BB93551	targeted	Absent	Sample	Sspa_Oph2007_COI	BD50	COI	45	BB93551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD50	GAAGGCCTTCCGATGATC	153.1647
BB93551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37878	WGS84	BB93551	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93551_Upin_Bot2011_COI	BD50	1	BB93551_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93551	Sample	BB93551	targeted	Absent	Sample	Upin_Bot2011_COI	BD50	COI	45	BB93551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD50	CTTTAATTACAGCGTTTTTATTGTTGT	153.1647
BB93552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40307	WGS84	BB93552	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93552_Mgig_Bot2012_COI	BD35	1	BB93552_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93552	Sample	BB93552	targeted	Absent	Sample	Mgig_Bot2012_COI	BD35	COI	45	BB93552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD35	TCTTATTCGTTGGAGACTTTATAACCCT	153.15355
BB93553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43042	WGS84	BB93553	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93553_Cmae_Bot2011_COI	BD25	1	BB93553_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93553	Sample	BB93553	targeted	Absent	Sample	Cmae_Bot2011_COI	BD25	COI	45	BB93553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD25	ATGAACAGTCTATCCTCCTTTAG	153.12364
BB93553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43042	WGS84	BB93553	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93553_Msal_Bot2012_COI	BD25	1	BB93553_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93553	Sample	BB93553	targeted	Absent	Sample	Msal_Bot2012_COI	BD25	COI	45	BB93553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD25	GYTAGTTCCRATGATGTTAGCTG	153.12364
BB93555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3571	WGS84	BB93555	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93555_Aamu_Bax2006_COI	BD64	1	BB93555_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93555	Sample	BB93555	targeted	Absent	Sample	Aamu_Bax2006_COI	BD64	COI	45	BB93555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD64	GCACAACCGGGATCTTTACTTC	153.18065
BB93555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3571	WGS84	BB93555	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93555_Pcan_Bot2011_IGS	BD64	1	BB93555_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93555	Sample	BB93555	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD64	IGS	45	BB93555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD64	CGTAATCCTCAGTACTGGCTA	153.18065
BB93555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3571	WGS84	BB93555	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93555_Sspa_Oph2007_COI	BD64	1	BB93555_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93555	Sample	BB93555	targeted	Absent	Sample	Sspa_Oph2007_COI	BD64	COI	45	BB93555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD64	GAAGGCCTTCCGATGATC	153.18065
BB93555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3571	WGS84	BB93555	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93555_Upin_Bot2011_COI	BD64	1	BB93555_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93555	Sample	BB93555	targeted	Absent	Sample	Upin_Bot2011_COI	BD64	COI	45	BB93555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD64	CTTTAATTACAGCGTTTTTATTGTTGT	153.18065
BB93556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36005	WGS84	BB93556	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93556_Mgig_Bot2012_COI	BD62	1	BB93556_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93556	Sample	BB93556	targeted	Absent	Sample	Mgig_Bot2012_COI	BD62	COI	45	BB93556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD62	TCTTATTCGTTGGAGACTTTATAACCCT	153.17846
BB93557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36529	WGS84	BB93557	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93557_Cmae_Bot2011_COI	BD59	1	BB93557_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93557	Sample	BB93557	targeted	Absent	Sample	Cmae_Bot2011_COI	BD59	COI	45	BB93557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD59	ATGAACAGTCTATCCTCCTTTAG	153.17455
BB93557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36529	WGS84	BB93557	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93557_Msal_Bot2012_COI	BD59	1	BB93557_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93557	Sample	BB93557	targeted	Absent	Sample	Msal_Bot2012_COI	BD59	COI	45	BB93557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD59	GYTAGTTCCRATGATGTTAGCTG	153.17455
BB93559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37292	WGS84	BB93559	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93559_Aamu_Bax2006_COI	BD54	1	BB93559_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93559	Sample	BB93559	targeted	Absent	Sample	Aamu_Bax2006_COI	BD54	COI	45	BB93559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD54	GCACAACCGGGATCTTTACTTC	153.16914
BB93559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37292	WGS84	BB93559	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93559_Pcan_Bot2011_IGS	BD54	1	BB93559_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93559	Sample	BB93559	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD54	IGS	45	BB93559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD54	CGTAATCCTCAGTACTGGCTA	153.16914
BB93559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37292	WGS84	BB93559	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93559_Sspa_Oph2007_COI	BD54	1	BB93559_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93559	Sample	BB93559	targeted	Absent	Sample	Sspa_Oph2007_COI	BD54	COI	45	BB93559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD54	GAAGGCCTTCCGATGATC	153.16914
BB93559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37292	WGS84	BB93559	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93559_Upin_Bot2011_COI	BD54	1	BB93559_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93559	Sample	BB93559	targeted	Absent	Sample	Upin_Bot2011_COI	BD54	COI	45	BB93559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD54	CTTTAATTACAGCGTTTTTATTGTTGT	153.16914
BB93560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37475	WGS84	BB93560	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93560_Cmae_Bot2011_COI	BD53	1	BB93560_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93560	Sample	BB93560	targeted	Absent	Sample	Cmae_Bot2011_COI	BD53	COI	45	BB93560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD53	ATGAACAGTCTATCCTCCTTTAG	153.16829
BB93560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37475	WGS84	BB93560	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93560_Msal_Bot2012_COI	BD53	1	BB93560_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93560	Sample	BB93560	targeted	Absent	Sample	Msal_Bot2012_COI	BD53	COI	45	BB93560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD53	GYTAGTTCCRATGATGTTAGCTG	153.16829
BB93562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38117	WGS84	BB93562	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93562_Aamu_Bax2006_COI	BD48	1	BB93562_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93562	Sample	BB93562	targeted	Absent	Sample	Aamu_Bax2006_COI	BD48	COI	45	BB93562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD48	GCACAACCGGGATCTTTACTTC	153.16243
BB93562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38117	WGS84	BB93562	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93562_Pcan_Bot2011_IGS	BD48	1	BB93562_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93562	Sample	BB93562	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD48	IGS	45	BB93562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD48	CGTAATCCTCAGTACTGGCTA	153.16243
BB93562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38117	WGS84	BB93562	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93562_Sspa_Oph2007_COI	BD48	1	BB93562_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93562	Sample	BB93562	targeted	Absent	Sample	Sspa_Oph2007_COI	BD48	COI	45	BB93562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD48	GAAGGCCTTCCGATGATC	153.16243
BB93562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38117	WGS84	BB93562	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93562_Upin_Bot2011_COI	BD48	1	BB93562_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93562	Sample	BB93562	targeted	Absent	Sample	Upin_Bot2011_COI	BD48	COI	45	BB93562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD48	CTTTAATTACAGCGTTTTTATTGTTGT	153.16243
BB93563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38398	WGS84	BB93563	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93563_Mgig_Bot2012_COI	BD46	1	BB93563_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93563	Sample	BB93563	targeted	Absent	Sample	Mgig_Bot2012_COI	BD46	COI	45	BB93563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD46	TCTTATTCGTTGGAGACTTTATAACCCT	153.16056
BB93564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38628	WGS84	BB93564	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93564_Cmae_Bot2011_COI	BD44	1	BB93564_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93564	Sample	BB93564	targeted	Absent	Sample	Cmae_Bot2011_COI	BD44	COI	45	BB93564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD44	ATGAACAGTCTATCCTCCTTTAG	153.15955
BB93564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38628	WGS84	BB93564	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93564_Msal_Bot2012_COI	BD44	1	BB93564_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93564	Sample	BB93564	targeted	Absent	Sample	Msal_Bot2012_COI	BD44	COI	45	BB93564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD44	GYTAGTTCCRATGATGTTAGCTG	153.15955
BB93566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40067	WGS84	BB93566	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93566_Aamu_Bax2006_COI	BD41	1	BB93566_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93566	Sample	BB93566	targeted	Absent	Sample	Aamu_Bax2006_COI	BD41	COI	45	BB93566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD41	GCACAACCGGGATCTTTACTTC	153.16346
BB93566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40067	WGS84	BB93566	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93566_Pcan_Bot2011_IGS	BD41	1	BB93566_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93566	Sample	BB93566	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD41	IGS	45	BB93566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD41	CGTAATCCTCAGTACTGGCTA	153.16346
BB93566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40067	WGS84	BB93566	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93566_Sspa_Oph2007_COI	BD41	1	BB93566_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93566	Sample	BB93566	targeted	Absent	Sample	Sspa_Oph2007_COI	BD41	COI	45	BB93566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD41	GAAGGCCTTCCGATGATC	153.16346
BB93566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40067	WGS84	BB93566	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93566_Upin_Bot2011_COI	BD41	1	BB93566_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93566	Sample	BB93566	targeted	Absent	Sample	Upin_Bot2011_COI	BD41	COI	45	BB93566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD41	CTTTAATTACAGCGTTTTTATTGTTGT	153.16346
BB93567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40091	WGS84	BB93567	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93567_Mgig_Bot2012_COI	BD39	1	BB93567_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93567	Sample	BB93567	targeted	Absent	Sample	Mgig_Bot2012_COI	BD39	COI	45	BB93567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD39	TCTTATTCGTTGGAGACTTTATAACCCT	153.16222
BB93568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40102	WGS84	BB93568	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93568_Cmae_Bot2011_COI	BD37	1	BB93568_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93568	Sample	BB93568	targeted	Absent	Sample	Cmae_Bot2011_COI	BD37	COI	45	BB93568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD37	ATGAACAGTCTATCCTCCTTTAG	153.15883
BB93568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40102	WGS84	BB93568	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93568_Msal_Bot2012_COI	BD37	1	BB93568_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93568	Sample	BB93568	targeted	Absent	Sample	Msal_Bot2012_COI	BD37	COI	45	BB93568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD37	GYTAGTTCCRATGATGTTAGCTG	153.15883
BB93570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.4123	WGS84	BB93570	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93570_Mgig_Bot2012_COI	BD32	1	BB93570_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93570	Sample	BB93570	targeted	Absent	Sample	Mgig_Bot2012_COI	BD32	COI	45	BB93570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD32	TCTTATTCGTTGGAGACTTTATAACCCT	153.14395
BB93571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.41593	WGS84	BB93571	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93571_Cmae_Bot2011_COI	BD31	1	BB93571_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93571	Sample	BB93571	targeted	Absent	Sample	Cmae_Bot2011_COI	BD31	COI	45	BB93571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD31	ATGAACAGTCTATCCTCCTTTAG	153.14258
BB93571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.41593	WGS84	BB93571	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93571_Msal_Bot2012_COI	BD31	1	BB93571_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93571	Sample	BB93571	targeted	Absent	Sample	Msal_Bot2012_COI	BD31	COI	45	BB93571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD31	GYTAGTTCCRATGATGTTAGCTG	153.14258
BB93573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42503	WGS84	BB93573	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93573_Aamu_Bax2006_COI	BD27	1	BB93573_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93573	Sample	BB93573	targeted	Absent	Sample	Aamu_Bax2006_COI	BD27	COI	45	BB93573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD27	GCACAACCGGGATCTTTACTTC	153.12793
BB93573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42503	WGS84	BB93573	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93573_Pcan_Bot2011_IGS	BD27	1	BB93573_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93573	Sample	BB93573	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD27	IGS	45	BB93573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD27	CGTAATCCTCAGTACTGGCTA	153.12793
BB93573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42503	WGS84	BB93573	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93573_Sspa_Oph2007_COI	BD27	1	BB93573_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93573	Sample	BB93573	targeted	Absent	Sample	Sspa_Oph2007_COI	BD27	COI	45	BB93573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD27	GAAGGCCTTCCGATGATC	153.12793
BB93573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42503	WGS84	BB93573	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93573_Upin_Bot2011_COI	BD27	1	BB93573_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93573	Sample	BB93573	targeted	Absent	Sample	Upin_Bot2011_COI	BD27	COI	45	BB93573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD27	CTTTAATTACAGCGTTTTTATTGTTGT	153.12793
BB93574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.428	WGS84	BB93574	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93574_Mgig_Bot2012_COI	BD24	1	BB93574_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93574	Sample	BB93574	targeted	Absent	Sample	Mgig_Bot2012_COI	BD24	COI	45	BB93574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD24	TCTTATTCGTTGGAGACTTTATAACCCT	153.12199
BB93575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42982	WGS84	BB93575	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93575_Cmae_Bot2011_COI	BD21	1	BB93575_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93575	Sample	BB93575	targeted	Absent	Sample	Cmae_Bot2011_COI	BD21	COI	45	BB93575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD21	ATGAACAGTCTATCCTCCTTTAG	153.12339
BB93575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42982	WGS84	BB93575	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93575_Msal_Bot2012_COI	BD21	1	BB93575_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93575	Sample	BB93575	targeted	Absent	Sample	Msal_Bot2012_COI	BD21	COI	45	BB93575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD21	GYTAGTTCCRATGATGTTAGCTG	153.12339
BB93577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44736	WGS84	BB93577	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93577_Aamu_Bax2006_COI	BD16	1	BB93577_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93577	Sample	BB93577	targeted	Absent	Sample	Aamu_Bax2006_COI	BD16	COI	45	BB93577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD16	GCACAACCGGGATCTTTACTTC	153.09726
BB93577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44736	WGS84	BB93577	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93577_Pcan_Bot2011_IGS	BD16	1	BB93577_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93577	Sample	BB93577	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD16	IGS	45	BB93577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD16	CGTAATCCTCAGTACTGGCTA	153.09726
BB93577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44736	WGS84	BB93577	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93577_Sspa_Oph2007_COI	BD16	1	BB93577_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93577	Sample	BB93577	targeted	Absent	Sample	Sspa_Oph2007_COI	BD16	COI	45	BB93577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD16	GAAGGCCTTCCGATGATC	153.09726
BB93577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44736	WGS84	BB93577	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93577_Upin_Bot2011_COI	BD16	1	BB93577_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93577	Sample	BB93577	targeted	Absent	Sample	Upin_Bot2011_COI	BD16	COI	45	BB93577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD16	CTTTAATTACAGCGTTTTTATTGTTGT	153.09726
BB93578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44561	WGS84	BB93578	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93578_Mgig_Bot2012_COI	BD14	1	BB93578_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93578	Sample	BB93578	targeted	Absent	Sample	Mgig_Bot2012_COI	BD14	COI	45	BB93578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD14	TCTTATTCGTTGGAGACTTTATAACCCT	153.08298
BB93579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44615	WGS84	BB93579	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93579_Cmae_Bot2011_COI	BD9	1	BB93579_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93579	Sample	BB93579	targeted	Absent	Sample	Cmae_Bot2011_COI	BD9	COI	45	BB93579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD9	ATGAACAGTCTATCCTCCTTTAG	153.07929
BB93579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44615	WGS84	BB93579	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93579_Msal_Bot2012_COI	BD9	1	BB93579_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93579	Sample	BB93579	targeted	Absent	Sample	Msal_Bot2012_COI	BD9	COI	45	BB93579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD9	GYTAGTTCCRATGATGTTAGCTG	153.07929
BB93580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44522	WGS84	BB93580	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93580_Aamu_Bax2006_COI	BD8	1	BB93580_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93580	Sample	BB93580	targeted	Absent	Sample	Aamu_Bax2006_COI	BD8	COI	45	BB93580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD8	GCACAACCGGGATCTTTACTTC	153.0791
BB93580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44522	WGS84	BB93580	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93580_Pcan_Bot2011_IGS	BD8	1	BB93580_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93580	Sample	BB93580	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD8	IGS	45	BB93580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD8	CGTAATCCTCAGTACTGGCTA	153.0791
BB93580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44522	WGS84	BB93580	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93580_Sspa_Oph2007_COI	BD8	1	BB93580_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93580	Sample	BB93580	targeted	Absent	Sample	Sspa_Oph2007_COI	BD8	COI	45	BB93580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD8	GAAGGCCTTCCGATGATC	153.0791
BB93580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44522	WGS84	BB93580	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93580_Upin_Bot2011_COI	BD8	1	BB93580_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93580	Sample	BB93580	targeted	Absent	Sample	Upin_Bot2011_COI	BD8	COI	45	BB93580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD8	CTTTAATTACAGCGTTTTTATTGTTGT	153.0791
BB93581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44533	WGS84	BB93581	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93581_Mgig_Bot2012_COI	BD7	1	BB93581_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93581	Sample	BB93581	targeted	Absent	Sample	Mgig_Bot2012_COI	BD7	COI	45	BB93581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD7	TCTTATTCGTTGGAGACTTTATAACCCT	153.07768
BB93582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44446	WGS84	BB93582	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93582_Cmae_Bot2011_COI	BD6	1	BB93582_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93582	Sample	BB93582	targeted	Absent	Sample	Cmae_Bot2011_COI	BD6	COI	45	BB93582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD6	ATGAACAGTCTATCCTCCTTTAG	153.07696
BB93582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44446	WGS84	BB93582	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93582_Msal_Bot2012_COI	BD6	1	BB93582_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93582	Sample	BB93582	targeted	Absent	Sample	Msal_Bot2012_COI	BD6	COI	45	BB93582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD6	GYTAGTTCCRATGATGTTAGCTG	153.07696
BB93584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44385	WGS84	BB93584	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93584_Aamu_Bax2006_COI	BD3	1	BB93584_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93584	Sample	BB93584	targeted	Absent	Sample	Aamu_Bax2006_COI	BD3	COI	45	BB93584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD3	GCACAACCGGGATCTTTACTTC	153.07508
BB93584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44385	WGS84	BB93584	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93584_Pcan_Bot2011_IGS	BD3	1	BB93584_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93584	Sample	BB93584	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD3	IGS	45	BB93584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD3	CGTAATCCTCAGTACTGGCTA	153.07508
BB93584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44385	WGS84	BB93584	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93584_Sspa_Oph2007_COI	BD3	1	BB93584_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93584	Sample	BB93584	targeted	Absent	Sample	Sspa_Oph2007_COI	BD3	COI	45	BB93584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD3	GAAGGCCTTCCGATGATC	153.07508
BB93584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44385	WGS84	BB93584	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93584_Upin_Bot2011_COI	BD3	1	BB93584_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93584	Sample	BB93584	targeted	Absent	Sample	Upin_Bot2011_COI	BD3	COI	45	BB93584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD3	CTTTAATTACAGCGTTTTTATTGTTGT	153.07508
BB93585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44293	WGS84	BB93585	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93585_Mgig_Bot2012_COI	BD2	1	BB93585_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93585	Sample	BB93585	targeted	Absent	Sample	Mgig_Bot2012_COI	BD2	COI	45	BB93585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD2	TCTTATTCGTTGGAGACTTTATAACCCT	153.07323
BB93586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24295	WGS84	BB93586	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93586_Cmae_Bot2011_COI	YD140	1	BB93586_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93586	Sample	BB93586	targeted	Absent	Sample	Cmae_Bot2011_COI	YD140	COI	45	BB93586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD140	ATGAACAGTCTATCCTCCTTTAG	149.30158
BB93586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24295	WGS84	BB93586	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93586_Msal_Bot2012_COI	YD140	1	BB93586_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93586	Sample	BB93586	targeted	Absent	Sample	Msal_Bot2012_COI	YD140	COI	45	BB93586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD140	GYTAGTTCCRATGATGTTAGCTG	149.30158
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93588_Aamu_Bax2006_COI	YD136	1	BB93588_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Aamu_Bax2006_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	GCACAACCGGGATCTTTACTTC	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93588_Pcan_Bot2011_IGS	YD136	1	BB93588_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD136	IGS	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	CGTAATCCTCAGTACTGGCTA	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93588_Pper_Dia2013_COI	YD136	1	BB93588_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Pper_Dia2013_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	CTTAGTGGCATTAATTCGDAATCC	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93588_Sspa_Oph2007_COI	YD136	1	BB93588_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Sspa_Oph2007_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	GAAGGCCTTCCGATGATC	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93588_Upin_Bot2011_COI	YD136	1	BB93588_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Upin_Bot2011_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	CTTTAATTACAGCGTTTTTATTGTTGT	149.31324
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93589_Cjap_Sim2018_COI	YD134	1	BB93589_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Cjap_Sim2018_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93589_Esin_And2018_Cyb	YD134	1	BB93589_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Esin_And2018_Cyb	YD134	Cytb	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	ACCCCTCCTCATATCCAACCA	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93589_Mgig_Bot2012_COI	YD134	1	BB93589_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Mgig_Bot2012_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	TCTTATTCGTTGGAGACTTTATAACCCT	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93589_Mjap_Gib2022_COI	YD134	1	BB93589_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Mjap_Gib2022_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	AATTTAATTGTGACTAGGCATGGTCTG	149.31226
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93591_Aamu_Bax2006_COI	YD131	1	BB93591_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Aamu_Bax2006_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	GCACAACCGGGATCTTTACTTC	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93591_Pcan_Bot2011_IGS	YD131	1	BB93591_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD131	IGS	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	CGTAATCCTCAGTACTGGCTA	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93591_Pper_Dia2013_COI	YD131	1	BB93591_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Pper_Dia2013_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	CTTAGTGGCATTAATTCGDAATCC	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93591_Sspa_Oph2007_COI	YD131	1	BB93591_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Sspa_Oph2007_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	GAAGGCCTTCCGATGATC	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93591_Upin_Bot2011_COI	YD131	1	BB93591_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Upin_Bot2011_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	CTTTAATTACAGCGTTTTTATTGTTGT	149.3143
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93592_Cjap_Sim2018_COI	YD129	1	BB93592_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Cjap_Sim2018_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93592_Esin_And2018_Cyb	YD129	1	BB93592_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Esin_And2018_Cyb	YD129	Cytb	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	ACCCCTCCTCATATCCAACCA	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93592_Mgig_Bot2012_COI	YD129	1	BB93592_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Mgig_Bot2012_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	TCTTATTCGTTGGAGACTTTATAACCCT	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93592_Mjap_Gib2022_COI	YD129	1	BB93592_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Mjap_Gib2022_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	AATTTAATTGTGACTAGGCATGGTCTG	149.31412
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93593_Cmae_Bot2011_COI	YD128	1	BB93593_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Cmae_Bot2011_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	ATGAACAGTCTATCCTCCTTTAG	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93593_Hsan_Wil2021_COI	YD128	1	BB93593_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Hsan_Wil2021_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	CCTGGGCCGGTATAGTAGGT	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93593_Mros_Gib2022_COI	YD128	1	BB93593_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Mros_Gib2022_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93593_Msal_Bot2012_COI	YD128	1	BB93593_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Msal_Bot2012_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	GYTAGTTCCRATGATGTTAGCTG	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93593_Pvir_Dia2013_COI	YD128	1	BB93593_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Pvir_Dia2013_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	CTTAGTGGCATTAATTCGDAATCC	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93593_Rhar_Sim2018_COI	YD128	1	BB93593_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Rhar_Sim2018_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	CTGCTGCTATTGCTCATG	149.31464
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93595_Aamu_Bax2006_COI	YD124	1	BB93595_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Aamu_Bax2006_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	GCACAACCGGGATCTTTACTTC	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93595_Pcan_Bot2011_IGS	YD124	1	BB93595_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD124	IGS	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	CGTAATCCTCAGTACTGGCTA	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93595_Pper_Dia2013_COI	YD124	1	BB93595_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Pper_Dia2013_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	CTTAGTGGCATTAATTCGDAATCC	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93595_Sspa_Oph2007_COI	YD124	1	BB93595_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Sspa_Oph2007_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	GAAGGCCTTCCGATGATC	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93595_Upin_Bot2011_COI	YD124	1	BB93595_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Upin_Bot2011_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	CTTTAATTACAGCGTTTTTATTGTTGT	149.31554
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93596_Cjap_Sim2018_COI	YD123	1	BB93596_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Cjap_Sim2018_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93596_Esin_And2018_Cyb	YD123	1	BB93596_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Esin_And2018_Cyb	YD123	Cytb	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	ACCCCTCCTCATATCCAACCA	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93596_Mgig_Bot2012_COI	YD123	1	BB93596_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Mgig_Bot2012_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	TCTTATTCGTTGGAGACTTTATAACCCT	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93596_Mjap_Gib2022_COI	YD123	1	BB93596_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Mjap_Gib2022_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	AATTTAATTGTGACTAGGCATGGTCTG	149.31475
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93597_Cmae_Bot2011_COI	YD122	1	BB93597_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Cmae_Bot2011_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	ATGAACAGTCTATCCTCCTTTAG	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93597_Hsan_Wil2021_COI	YD122	1	BB93597_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Hsan_Wil2021_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	CCTGGGCCGGTATAGTAGGT	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93597_Mros_Gib2022_COI	YD122	1	BB93597_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Mros_Gib2022_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93597_Msal_Bot2012_COI	YD122	1	BB93597_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Msal_Bot2012_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	GYTAGTTCCRATGATGTTAGCTG	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93597_Pvir_Dia2013_COI	YD122	1	BB93597_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Pvir_Dia2013_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	CTTAGTGGCATTAATTCGDAATCC	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93597_Rhar_Sim2018_COI	YD122	1	BB93597_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Rhar_Sim2018_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	CTGCTGCTATTGCTCATG	149.31396
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93599_Aamu_Bax2006_COI	YD117	1	BB93599_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Aamu_Bax2006_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	GCACAACCGGGATCTTTACTTC	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93599_Pcan_Bot2011_IGS	YD117	1	BB93599_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD117	IGS	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	CGTAATCCTCAGTACTGGCTA	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93599_Pper_Dia2013_COI	YD117	1	BB93599_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Pper_Dia2013_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	CTTAGTGGCATTAATTCGDAATCC	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93599_Sspa_Oph2007_COI	YD117	1	BB93599_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Sspa_Oph2007_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	GAAGGCCTTCCGATGATC	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93599_Upin_Bot2011_COI	YD117	1	BB93599_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Upin_Bot2011_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	CTTTAATTACAGCGTTTTTATTGTTGT	149.31395
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93600_Cjap_Sim2018_COI	YD116	1	BB93600_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Cjap_Sim2018_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93600_Esin_And2018_Cyb	YD116	1	BB93600_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Esin_And2018_Cyb	YD116	Cytb	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	ACCCCTCCTCATATCCAACCA	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93600_Mgig_Bot2012_COI	YD116	1	BB93600_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Mgig_Bot2012_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	TCTTATTCGTTGGAGACTTTATAACCCT	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93600_Mjap_Gib2022_COI	YD116	1	BB93600_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Mjap_Gib2022_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	AATTTAATTGTGACTAGGCATGGTCTG	149.31627
BB93601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26553	WGS84	BB93601	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93601_Cmae_Bot2011_COI	YD114	1	BB93601_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93601	Sample	BB93601	targeted	Absent	Sample	Cmae_Bot2011_COI	YD114	COI	45	BB93601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD114	ATGAACAGTCTATCCTCCTTTAG	149.31434
BB93601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26553	WGS84	BB93601	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93601_Msal_Bot2012_COI	YD114	1	BB93601_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93601	Sample	BB93601	targeted	Absent	Sample	Msal_Bot2012_COI	YD114	COI	45	BB93601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD114	GYTAGTTCCRATGATGTTAGCTG	149.31434
BB93603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25606	WGS84	BB93603	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93603_Aamu_Bax2006_COI	YD110	1	BB93603_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93603	Sample	BB93603	targeted	Absent	Sample	Aamu_Bax2006_COI	YD110	COI	45	BB93603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD110	GCACAACCGGGATCTTTACTTC	149.30718
BB93603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25606	WGS84	BB93603	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93603_Pcan_Bot2011_IGS	YD110	1	BB93603_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93603	Sample	BB93603	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD110	IGS	45	BB93603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD110	CGTAATCCTCAGTACTGGCTA	149.30718
BB93603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25606	WGS84	BB93603	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93603_Sspa_Oph2007_COI	YD110	1	BB93603_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93603	Sample	BB93603	targeted	Absent	Sample	Sspa_Oph2007_COI	YD110	COI	45	BB93603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD110	GAAGGCCTTCCGATGATC	149.30718
BB93603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25606	WGS84	BB93603	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93603_Upin_Bot2011_COI	YD110	1	BB93603_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93603	Sample	BB93603	targeted	Absent	Sample	Upin_Bot2011_COI	YD110	COI	45	BB93603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD110	CTTTAATTACAGCGTTTTTATTGTTGT	149.30718
BB93604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25593	WGS84	BB93604	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93604_Mgig_Bot2012_COI	YD108	1	BB93604_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93604	Sample	BB93604	targeted	Absent	Sample	Mgig_Bot2012_COI	YD108	COI	45	BB93604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD108	TCTTATTCGTTGGAGACTTTATAACCCT	149.30946
BB93605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26828	WGS84	BB93605	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93605_Cmae_Bot2011_COI	YD107	1	BB93605_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93605	Sample	BB93605	targeted	Absent	Sample	Cmae_Bot2011_COI	YD107	COI	45	BB93605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD107	ATGAACAGTCTATCCTCCTTTAG	149.31404
BB93605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26828	WGS84	BB93605	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93605_Msal_Bot2012_COI	YD107	1	BB93605_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93605	Sample	BB93605	targeted	Absent	Sample	Msal_Bot2012_COI	YD107	COI	45	BB93605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD107	GYTAGTTCCRATGATGTTAGCTG	149.31404
BB93607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26817	WGS84	BB93607	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93607_Aamu_Bax2006_COI	YD105	1	BB93607_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93607	Sample	BB93607	targeted	Absent	Sample	Aamu_Bax2006_COI	YD105	COI	45	BB93607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD105	GCACAACCGGGATCTTTACTTC	149.31344
BB93607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26817	WGS84	BB93607	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93607_Pcan_Bot2011_IGS	YD105	1	BB93607_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93607	Sample	BB93607	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD105	IGS	45	BB93607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD105	CGTAATCCTCAGTACTGGCTA	149.31344
BB93607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26817	WGS84	BB93607	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93607_Sspa_Oph2007_COI	YD105	1	BB93607_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93607	Sample	BB93607	targeted	Absent	Sample	Sspa_Oph2007_COI	YD105	COI	45	BB93607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD105	GAAGGCCTTCCGATGATC	149.31344
BB93607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26817	WGS84	BB93607	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93607_Upin_Bot2011_COI	YD105	1	BB93607_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93607	Sample	BB93607	targeted	Absent	Sample	Upin_Bot2011_COI	YD105	COI	45	BB93607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD105	CTTTAATTACAGCGTTTTTATTGTTGT	149.31344
BB93608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25174	WGS84	BB93608	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93608_Mgig_Bot2012_COI	YD103	1	BB93608_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93608	Sample	BB93608	targeted	Absent	Sample	Mgig_Bot2012_COI	YD103	COI	45	BB93608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD103	TCTTATTCGTTGGAGACTTTATAACCCT	149.30413
BB93609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25226	WGS84	BB93609	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93609_Cmae_Bot2011_COI	YD101	1	BB93609_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93609	Sample	BB93609	targeted	Absent	Sample	Cmae_Bot2011_COI	YD101	COI	45	BB93609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD101	ATGAACAGTCTATCCTCCTTTAG	149.30554
BB93609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25226	WGS84	BB93609	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93609_Msal_Bot2012_COI	YD101	1	BB93609_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93609	Sample	BB93609	targeted	Absent	Sample	Msal_Bot2012_COI	YD101	COI	45	BB93609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD101	GYTAGTTCCRATGATGTTAGCTG	149.30554
BB93610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25367	WGS84	BB93610	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93610_Aamu_Bax2006_COI	YD99	1	BB93610_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93610	Sample	BB93610	targeted	Absent	Sample	Aamu_Bax2006_COI	YD99	COI	45	BB93610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD99	GCACAACCGGGATCTTTACTTC	149.30558
BB93610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25367	WGS84	BB93610	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93610_Pcan_Bot2011_IGS	YD99	1	BB93610_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93610	Sample	BB93610	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD99	IGS	45	BB93610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD99	CGTAATCCTCAGTACTGGCTA	149.30558
BB93610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25367	WGS84	BB93610	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93610_Sspa_Oph2007_COI	YD99	1	BB93610_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93610	Sample	BB93610	targeted	Absent	Sample	Sspa_Oph2007_COI	YD99	COI	45	BB93610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD99	GAAGGCCTTCCGATGATC	149.30558
BB93610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25367	WGS84	BB93610	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93610_Upin_Bot2011_COI	YD99	1	BB93610_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93610	Sample	BB93610	targeted	Absent	Sample	Upin_Bot2011_COI	YD99	COI	45	BB93610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD99	CTTTAATTACAGCGTTTTTATTGTTGT	149.30558
BB93611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25356	WGS84	BB93611	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93611_Mgig_Bot2012_COI	YD98	1	BB93611_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93611	Sample	BB93611	targeted	Absent	Sample	Mgig_Bot2012_COI	YD98	COI	45	BB93611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD98	TCTTATTCGTTGGAGACTTTATAACCCT	149.30649
BB93612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25435	WGS84	BB93612	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93612_Cmae_Bot2011_COI	YD97	1	BB93612_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93612	Sample	BB93612	targeted	Absent	Sample	Cmae_Bot2011_COI	YD97	COI	45	BB93612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD97	ATGAACAGTCTATCCTCCTTTAG	149.30611
BB93612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25435	WGS84	BB93612	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93612_Msal_Bot2012_COI	YD97	1	BB93612_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93612	Sample	BB93612	targeted	Absent	Sample	Msal_Bot2012_COI	YD97	COI	45	BB93612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD97	GYTAGTTCCRATGATGTTAGCTG	149.30611
BB93614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25563	WGS84	BB93614	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93614_Aamu_Bax2006_COI	YD94	1	BB93614_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93614	Sample	BB93614	targeted	Absent	Sample	Aamu_Bax2006_COI	YD94	COI	45	BB93614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD94	GCACAACCGGGATCTTTACTTC	149.30793
BB93614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25563	WGS84	BB93614	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93614_Pcan_Bot2011_IGS	YD94	1	BB93614_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93614	Sample	BB93614	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD94	IGS	45	BB93614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD94	CGTAATCCTCAGTACTGGCTA	149.30793
BB93614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25563	WGS84	BB93614	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93614_Sspa_Oph2007_COI	YD94	1	BB93614_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93614	Sample	BB93614	targeted	Absent	Sample	Sspa_Oph2007_COI	YD94	COI	45	BB93614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD94	GAAGGCCTTCCGATGATC	149.30793
BB93614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25563	WGS84	BB93614	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93614_Upin_Bot2011_COI	YD94	1	BB93614_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93614	Sample	BB93614	targeted	Absent	Sample	Upin_Bot2011_COI	YD94	COI	45	BB93614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD94	CTTTAATTACAGCGTTTTTATTGTTGT	149.30793
BB93615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25704	WGS84	BB93615	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93615_Mgig_Bot2012_COI	YD92	1	BB93615_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93615	Sample	BB93615	targeted	Absent	Sample	Mgig_Bot2012_COI	YD92	COI	45	BB93615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD92	TCTTATTCGTTGGAGACTTTATAACCCT	149.30826
BB93616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25693	WGS84	BB93616	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93616_Cmae_Bot2011_COI	YD91	1	BB93616_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93616	Sample	BB93616	targeted	Absent	Sample	Cmae_Bot2011_COI	YD91	COI	45	BB93616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD91	ATGAACAGTCTATCCTCCTTTAG	149.30923
BB93616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25693	WGS84	BB93616	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93616_Msal_Bot2012_COI	YD91	1	BB93616_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93616	Sample	BB93616	targeted	Absent	Sample	Msal_Bot2012_COI	YD91	COI	45	BB93616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD91	GYTAGTTCCRATGATGTTAGCTG	149.30923
BB93618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2592	WGS84	BB93618	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93618_Aamu_Bax2006_COI	YD88	1	BB93618_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93618	Sample	BB93618	targeted	Absent	Sample	Aamu_Bax2006_COI	YD88	COI	45	BB93618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD88	GCACAACCGGGATCTTTACTTC	149.30901
BB93618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2592	WGS84	BB93618	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93618_Pcan_Bot2011_IGS	YD88	1	BB93618_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93618	Sample	BB93618	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD88	IGS	45	BB93618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD88	CGTAATCCTCAGTACTGGCTA	149.30901
BB93618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2592	WGS84	BB93618	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93618_Sspa_Oph2007_COI	YD88	1	BB93618_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93618	Sample	BB93618	targeted	Absent	Sample	Sspa_Oph2007_COI	YD88	COI	45	BB93618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD88	GAAGGCCTTCCGATGATC	149.30901
BB93618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2592	WGS84	BB93618	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93618_Upin_Bot2011_COI	YD88	1	BB93618_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93618	Sample	BB93618	targeted	Absent	Sample	Upin_Bot2011_COI	YD88	COI	45	BB93618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD88	CTTTAATTACAGCGTTTTTATTGTTGT	149.30901
BB93619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26054	WGS84	BB93619	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93619_Mgig_Bot2012_COI	YD85	1	BB93619_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93619	Sample	BB93619	targeted	Absent	Sample	Mgig_Bot2012_COI	YD85	COI	45	BB93619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD85	TCTTATTCGTTGGAGACTTTATAACCCT	149.31013
BB93621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26044	WGS84	BB93621	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93621_Aamu_Bax2006_COI	YD83	1	BB93621_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93621	Sample	BB93621	targeted	Absent	Sample	Aamu_Bax2006_COI	YD83	COI	45	BB93621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD83	GCACAACCGGGATCTTTACTTC	149.31104
BB93621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26044	WGS84	BB93621	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93621_Pcan_Bot2011_IGS	YD83	1	BB93621_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93621	Sample	BB93621	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD83	IGS	45	BB93621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD83	CGTAATCCTCAGTACTGGCTA	149.31104
BB93621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26044	WGS84	BB93621	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93621_Sspa_Oph2007_COI	YD83	1	BB93621_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93621	Sample	BB93621	targeted	Absent	Sample	Sspa_Oph2007_COI	YD83	COI	45	BB93621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD83	GAAGGCCTTCCGATGATC	149.31104
BB93621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26044	WGS84	BB93621	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93621_Upin_Bot2011_COI	YD83	1	BB93621_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93621	Sample	BB93621	targeted	Absent	Sample	Upin_Bot2011_COI	YD83	COI	45	BB93621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD83	CTTTAATTACAGCGTTTTTATTGTTGT	149.31104
BB93622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26411	WGS84	BB93622	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93622_Mgig_Bot2012_COI	YD81	1	BB93622_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93622	Sample	BB93622	targeted	Absent	Sample	Mgig_Bot2012_COI	YD81	COI	45	BB93622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD81	TCTTATTCGTTGGAGACTTTATAACCCT	149.31103
BB93623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26646	WGS84	BB93623	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93623_Cmae_Bot2011_COI	YD76	1	BB93623_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93623	Sample	BB93623	targeted	Absent	Sample	Cmae_Bot2011_COI	YD76	COI	45	BB93623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD76	ATGAACAGTCTATCCTCCTTTAG	149.3169
BB93623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26646	WGS84	BB93623	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93623_Msal_Bot2012_COI	YD76	1	BB93623_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93623	Sample	BB93623	targeted	Absent	Sample	Msal_Bot2012_COI	YD76	COI	45	BB93623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD76	GYTAGTTCCRATGATGTTAGCTG	149.3169
BB93625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26792	WGS84	BB93625	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93625_Aamu_Bax2006_COI	YD74	1	BB93625_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93625	Sample	BB93625	targeted	Absent	Sample	Aamu_Bax2006_COI	YD74	COI	45	BB93625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD74	GCACAACCGGGATCTTTACTTC	149.31616
BB93625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26792	WGS84	BB93625	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93625_Pcan_Bot2011_IGS	YD74	1	BB93625_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93625	Sample	BB93625	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD74	IGS	45	BB93625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD74	CGTAATCCTCAGTACTGGCTA	149.31616
BB93625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26792	WGS84	BB93625	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93625_Sspa_Oph2007_COI	YD74	1	BB93625_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93625	Sample	BB93625	targeted	Absent	Sample	Sspa_Oph2007_COI	YD74	COI	45	BB93625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD74	GAAGGCCTTCCGATGATC	149.31616
BB93625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26792	WGS84	BB93625	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93625_Upin_Bot2011_COI	YD74	1	BB93625_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93625	Sample	BB93625	targeted	Absent	Sample	Upin_Bot2011_COI	YD74	COI	45	BB93625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD74	CTTTAATTACAGCGTTTTTATTGTTGT	149.31616
BB93626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2573	WGS84	BB93626	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93626_Mgig_Bot2012_COI	YD73	1	BB93626_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93626	Sample	BB93626	targeted	Absent	Sample	Mgig_Bot2012_COI	YD73	COI	45	BB93626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD73	TCTTATTCGTTGGAGACTTTATAACCCT	149.31246
BB93627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25588	WGS84	BB93627	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93627_Cmae_Bot2011_COI	YD71	1	BB93627_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93627	Sample	BB93627	targeted	Absent	Sample	Cmae_Bot2011_COI	YD71	COI	45	BB93627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD71	ATGAACAGTCTATCCTCCTTTAG	149.31227
BB93627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25588	WGS84	BB93627	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93627_Msal_Bot2012_COI	YD71	1	BB93627_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93627	Sample	BB93627	targeted	Absent	Sample	Msal_Bot2012_COI	YD71	COI	45	BB93627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD71	GYTAGTTCCRATGATGTTAGCTG	149.31227
BB93629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25774	WGS84	BB93629	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93629_Aamu_Bax2006_COI	YD69	1	BB93629_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93629	Sample	BB93629	targeted	Absent	Sample	Aamu_Bax2006_COI	YD69	COI	45	BB93629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD69	GCACAACCGGGATCTTTACTTC	149.31154
BB93629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25774	WGS84	BB93629	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93629_Pcan_Bot2011_IGS	YD69	1	BB93629_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93629	Sample	BB93629	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD69	IGS	45	BB93629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD69	CGTAATCCTCAGTACTGGCTA	149.31154
BB93629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25774	WGS84	BB93629	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93629_Sspa_Oph2007_COI	YD69	1	BB93629_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93629	Sample	BB93629	targeted	Absent	Sample	Sspa_Oph2007_COI	YD69	COI	45	BB93629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD69	GAAGGCCTTCCGATGATC	149.31154
BB93629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25774	WGS84	BB93629	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93629_Upin_Bot2011_COI	YD69	1	BB93629_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93629	Sample	BB93629	targeted	Absent	Sample	Upin_Bot2011_COI	YD69	COI	45	BB93629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD69	CTTTAATTACAGCGTTTTTATTGTTGT	149.31154
BB93630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2553	WGS84	BB93630	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93630_Cmae_Bot2011_COI	YD65	1	BB93630_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93630	Sample	BB93630	targeted	Absent	Sample	Cmae_Bot2011_COI	YD65	COI	45	BB93630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD65	ATGAACAGTCTATCCTCCTTTAG	149.31084
BB93630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2553	WGS84	BB93630	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93630_Msal_Bot2012_COI	YD65	1	BB93630_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93630	Sample	BB93630	targeted	Absent	Sample	Msal_Bot2012_COI	YD65	COI	45	BB93630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD65	GYTAGTTCCRATGATGTTAGCTG	149.31084
BB93632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25453	WGS84	BB93632	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93632_Aamu_Bax2006_COI	YD63	1	BB93632_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93632	Sample	BB93632	targeted	Absent	Sample	Aamu_Bax2006_COI	YD63	COI	45	BB93632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD63	GCACAACCGGGATCTTTACTTC	149.31127
BB93632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25453	WGS84	BB93632	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93632_Pcan_Bot2011_IGS	YD63	1	BB93632_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93632	Sample	BB93632	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD63	IGS	45	BB93632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD63	CGTAATCCTCAGTACTGGCTA	149.31127
BB93632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25453	WGS84	BB93632	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93632_Sspa_Oph2007_COI	YD63	1	BB93632_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93632	Sample	BB93632	targeted	Absent	Sample	Sspa_Oph2007_COI	YD63	COI	45	BB93632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD63	GAAGGCCTTCCGATGATC	149.31127
BB93632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25453	WGS84	BB93632	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93632_Upin_Bot2011_COI	YD63	1	BB93632_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93632	Sample	BB93632	targeted	Absent	Sample	Upin_Bot2011_COI	YD63	COI	45	BB93632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD63	CTTTAATTACAGCGTTTTTATTGTTGT	149.31127
BB93633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25462	WGS84	BB93633	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93633_Mgig_Bot2012_COI	YD62	1	BB93633_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93633	Sample	BB93633	targeted	Absent	Sample	Mgig_Bot2012_COI	YD62	COI	45	BB93633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD62	TCTTATTCGTTGGAGACTTTATAACCCT	149.3103
BB93634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25389	WGS84	BB93634	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93634_Cmae_Bot2011_COI	YD59	1	BB93634_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93634	Sample	BB93634	targeted	Absent	Sample	Cmae_Bot2011_COI	YD59	COI	45	BB93634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD59	ATGAACAGTCTATCCTCCTTTAG	149.31064
BB93634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25389	WGS84	BB93634	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93634_Msal_Bot2012_COI	YD59	1	BB93634_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93634	Sample	BB93634	targeted	Absent	Sample	Msal_Bot2012_COI	YD59	COI	45	BB93634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD59	GYTAGTTCCRATGATGTTAGCTG	149.31064
BB93636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25376	WGS84	BB93636	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93636_Aamu_Bax2006_COI	YD54	1	BB93636_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93636	Sample	BB93636	targeted	Absent	Sample	Aamu_Bax2006_COI	YD54	COI	45	BB93636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD54	GCACAACCGGGATCTTTACTTC	149.30868
BB93636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25376	WGS84	BB93636	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93636_Pcan_Bot2011_IGS	YD54	1	BB93636_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93636	Sample	BB93636	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD54	IGS	45	BB93636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD54	CGTAATCCTCAGTACTGGCTA	149.30868
BB93636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25376	WGS84	BB93636	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93636_Sspa_Oph2007_COI	YD54	1	BB93636_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93636	Sample	BB93636	targeted	Absent	Sample	Sspa_Oph2007_COI	YD54	COI	45	BB93636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD54	GAAGGCCTTCCGATGATC	149.30868
BB93636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25376	WGS84	BB93636	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93636_Upin_Bot2011_COI	YD54	1	BB93636_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93636	Sample	BB93636	targeted	Absent	Sample	Upin_Bot2011_COI	YD54	COI	45	BB93636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD54	CTTTAATTACAGCGTTTTTATTGTTGT	149.30868
BB93637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25107	WGS84	BB93637	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93637_Mgig_Bot2012_COI	YD52	1	BB93637_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93637	Sample	BB93637	targeted	Absent	Sample	Mgig_Bot2012_COI	YD52	COI	45	BB93637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD52	TCTTATTCGTTGGAGACTTTATAACCCT	149.30902
BB93638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25162	WGS84	BB93638	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93638_Cmae_Bot2011_COI	YD51	1	BB93638_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93638	Sample	BB93638	targeted	Absent	Sample	Cmae_Bot2011_COI	YD51	COI	45	BB93638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD51	ATGAACAGTCTATCCTCCTTTAG	149.3087
BB93638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25162	WGS84	BB93638	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93638_Msal_Bot2012_COI	YD51	1	BB93638_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93638	Sample	BB93638	targeted	Absent	Sample	Msal_Bot2012_COI	YD51	COI	45	BB93638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD51	GYTAGTTCCRATGATGTTAGCTG	149.3087
BB93640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24547	WGS84	BB93640	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93640_Mgig_Bot2012_COI	YD46	1	BB93640_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93640	Sample	BB93640	targeted	Absent	Sample	Mgig_Bot2012_COI	YD46	COI	45	BB93640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD46	TCTTATTCGTTGGAGACTTTATAACCCT	149.30575
BB93641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24705	WGS84	BB93641	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93641_Cmae_Bot2011_COI	YD44	1	BB93641_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93641	Sample	BB93641	targeted	Absent	Sample	Cmae_Bot2011_COI	YD44	COI	45	BB93641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD44	ATGAACAGTCTATCCTCCTTTAG	149.30487
BB93641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24705	WGS84	BB93641	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93641_Msal_Bot2012_COI	YD44	1	BB93641_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93641	Sample	BB93641	targeted	Absent	Sample	Msal_Bot2012_COI	YD44	COI	45	BB93641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD44	GYTAGTTCCRATGATGTTAGCTG	149.30487
BB93643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24635	WGS84	BB93643	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93643_Aamu_Bax2006_COI	YD41	1	BB93643_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93643	Sample	BB93643	targeted	Absent	Sample	Aamu_Bax2006_COI	YD41	COI	45	BB93643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD41	GCACAACCGGGATCTTTACTTC	149.30463
BB93643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24635	WGS84	BB93643	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93643_Pcan_Bot2011_IGS	YD41	1	BB93643_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93643	Sample	BB93643	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD41	IGS	45	BB93643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD41	CGTAATCCTCAGTACTGGCTA	149.30463
BB93643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24635	WGS84	BB93643	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93643_Sspa_Oph2007_COI	YD41	1	BB93643_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93643	Sample	BB93643	targeted	Absent	Sample	Sspa_Oph2007_COI	YD41	COI	45	BB93643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD41	GAAGGCCTTCCGATGATC	149.30463
BB93643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24635	WGS84	BB93643	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93643_Upin_Bot2011_COI	YD41	1	BB93643_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93643	Sample	BB93643	targeted	Absent	Sample	Upin_Bot2011_COI	YD41	COI	45	BB93643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD41	CTTTAATTACAGCGTTTTTATTGTTGT	149.30463
BB93644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24415	WGS84	BB93644	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93644_Mgig_Bot2012_COI	YD40	1	BB93644_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93644	Sample	BB93644	targeted	Absent	Sample	Mgig_Bot2012_COI	YD40	COI	45	BB93644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD40	TCTTATTCGTTGGAGACTTTATAACCCT	149.30462
BB93645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24561	WGS84	BB93645	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93645_Cmae_Bot2011_COI	YD38	1	BB93645_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93645	Sample	BB93645	targeted	Absent	Sample	Cmae_Bot2011_COI	YD38	COI	45	BB93645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD38	ATGAACAGTCTATCCTCCTTTAG	149.30415
BB93645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24561	WGS84	BB93645	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93645_Msal_Bot2012_COI	YD38	1	BB93645_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93645	Sample	BB93645	targeted	Absent	Sample	Msal_Bot2012_COI	YD38	COI	45	BB93645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD38	GYTAGTTCCRATGATGTTAGCTG	149.30415
BB93647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24356	WGS84	BB93647	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93647_Aamu_Bax2006_COI	YD35	1	BB93647_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93647	Sample	BB93647	targeted	Absent	Sample	Aamu_Bax2006_COI	YD35	COI	45	BB93647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD35	GCACAACCGGGATCTTTACTTC	149.30339
BB93647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24356	WGS84	BB93647	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93647_Pcan_Bot2011_IGS	YD35	1	BB93647_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93647	Sample	BB93647	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD35	IGS	45	BB93647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD35	CGTAATCCTCAGTACTGGCTA	149.30339
BB93647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24356	WGS84	BB93647	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93647_Sspa_Oph2007_COI	YD35	1	BB93647_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93647	Sample	BB93647	targeted	Absent	Sample	Sspa_Oph2007_COI	YD35	COI	45	BB93647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD35	GAAGGCCTTCCGATGATC	149.30339
BB93647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24356	WGS84	BB93647	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93647_Upin_Bot2011_COI	YD35	1	BB93647_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93647	Sample	BB93647	targeted	Absent	Sample	Upin_Bot2011_COI	YD35	COI	45	BB93647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD35	CTTTAATTACAGCGTTTTTATTGTTGT	149.30339
BB93648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24414	WGS84	BB93648	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93648_Mgig_Bot2012_COI	YD34	1	BB93648_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93648	Sample	BB93648	targeted	Absent	Sample	Mgig_Bot2012_COI	YD34	COI	45	BB93648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD34	TCTTATTCGTTGGAGACTTTATAACCCT	149.30384
BB93649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25118	WGS84	BB93649	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93649_Cmae_Bot2011_COI	YD26	1	BB93649_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93649	Sample	BB93649	targeted	Absent	Sample	Cmae_Bot2011_COI	YD26	COI	45	BB93649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD26	ATGAACAGTCTATCCTCCTTTAG	149.30754
BB93649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25118	WGS84	BB93649	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93649_Msal_Bot2012_COI	YD26	1	BB93649_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93649	Sample	BB93649	targeted	Absent	Sample	Msal_Bot2012_COI	YD26	COI	45	BB93649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD26	GYTAGTTCCRATGATGTTAGCTG	149.30754
BB93650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24972	WGS84	BB93650	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93650_Aamu_Bax2006_COI	YD24	1	BB93650_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93650	Sample	BB93650	targeted	Absent	Sample	Aamu_Bax2006_COI	YD24	COI	45	BB93650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD24	GCACAACCGGGATCTTTACTTC	149.30738
BB93650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24972	WGS84	BB93650	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93650_Pcan_Bot2011_IGS	YD24	1	BB93650_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93650	Sample	BB93650	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD24	IGS	45	BB93650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD24	CGTAATCCTCAGTACTGGCTA	149.30738
BB93650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24972	WGS84	BB93650	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93650_Sspa_Oph2007_COI	YD24	1	BB93650_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93650	Sample	BB93650	targeted	Absent	Sample	Sspa_Oph2007_COI	YD24	COI	45	BB93650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD24	GAAGGCCTTCCGATGATC	149.30738
BB93650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24972	WGS84	BB93650	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93650_Upin_Bot2011_COI	YD24	1	BB93650_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93650	Sample	BB93650	targeted	Absent	Sample	Upin_Bot2011_COI	YD24	COI	45	BB93650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD24	CTTTAATTACAGCGTTTTTATTGTTGT	149.30738
BB93651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24831	WGS84	BB93651	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93651_Mgig_Bot2012_COI	YD22	1	BB93651_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93651	Sample	BB93651	targeted	Absent	Sample	Mgig_Bot2012_COI	YD22	COI	45	BB93651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD22	TCTTATTCGTTGGAGACTTTATAACCCT	149.30725
BB93652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24989	WGS84	BB93652	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93652_Cmae_Bot2011_COI	YD20	1	BB93652_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93652	Sample	BB93652	targeted	Absent	Sample	Cmae_Bot2011_COI	YD20	COI	45	BB93652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD20	ATGAACAGTCTATCCTCCTTTAG	149.30652
BB93652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24989	WGS84	BB93652	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93652_Msal_Bot2012_COI	YD20	1	BB93652_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93652	Sample	BB93652	targeted	Absent	Sample	Msal_Bot2012_COI	YD20	COI	45	BB93652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD20	GYTAGTTCCRATGATGTTAGCTG	149.30652
BB93654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24845	WGS84	BB93654	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93654_Aamu_Bax2006_COI	YD18	1	BB93654_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93654	Sample	BB93654	targeted	Absent	Sample	Aamu_Bax2006_COI	YD18	COI	45	BB93654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD18	GCACAACCGGGATCTTTACTTC	149.3065
BB93654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24845	WGS84	BB93654	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93654_Pcan_Bot2011_IGS	YD18	1	BB93654_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93654	Sample	BB93654	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD18	IGS	45	BB93654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD18	CGTAATCCTCAGTACTGGCTA	149.3065
BB93654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24845	WGS84	BB93654	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93654_Sspa_Oph2007_COI	YD18	1	BB93654_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93654	Sample	BB93654	targeted	Absent	Sample	Sspa_Oph2007_COI	YD18	COI	45	BB93654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD18	GAAGGCCTTCCGATGATC	149.3065
BB93654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24845	WGS84	BB93654	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93654_Upin_Bot2011_COI	YD18	1	BB93654_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93654	Sample	BB93654	targeted	Absent	Sample	Upin_Bot2011_COI	YD18	COI	45	BB93654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD18	CTTTAATTACAGCGTTTTTATTGTTGT	149.3065
BB93655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24924	WGS84	BB93655	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93655_Mgig_Bot2012_COI	YD17	1	BB93655_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93655	Sample	BB93655	targeted	Absent	Sample	Mgig_Bot2012_COI	YD17	COI	45	BB93655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD17	TCTTATTCGTTGGAGACTTTATAACCCT	149.30586
BB93656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24951	WGS84	BB93656	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93656_Cmae_Bot2011_COI	YD15	1	BB93656_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93656	Sample	BB93656	targeted	Absent	Sample	Cmae_Bot2011_COI	YD15	COI	45	BB93656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD15	ATGAACAGTCTATCCTCCTTTAG	149.30338
BB93656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24951	WGS84	BB93656	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93656_Msal_Bot2012_COI	YD15	1	BB93656_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93656	Sample	BB93656	targeted	Absent	Sample	Msal_Bot2012_COI	YD15	COI	45	BB93656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD15	GYTAGTTCCRATGATGTTAGCTG	149.30338
BB93658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24742	WGS84	BB93658	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93658_Aamu_Bax2006_COI	YD12	1	BB93658_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93658	Sample	BB93658	targeted	Absent	Sample	Aamu_Bax2006_COI	YD12	COI	45	BB93658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD12	GCACAACCGGGATCTTTACTTC	149.30258
BB93658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24742	WGS84	BB93658	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93658_Pcan_Bot2011_IGS	YD12	1	BB93658_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93658	Sample	BB93658	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD12	IGS	45	BB93658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD12	CGTAATCCTCAGTACTGGCTA	149.30258
BB93658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24742	WGS84	BB93658	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93658_Sspa_Oph2007_COI	YD12	1	BB93658_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93658	Sample	BB93658	targeted	Absent	Sample	Sspa_Oph2007_COI	YD12	COI	45	BB93658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD12	GAAGGCCTTCCGATGATC	149.30258
BB93658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24742	WGS84	BB93658	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93658_Upin_Bot2011_COI	YD12	1	BB93658_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93658	Sample	BB93658	targeted	Absent	Sample	Upin_Bot2011_COI	YD12	COI	45	BB93658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD12	CTTTAATTACAGCGTTTTTATTGTTGT	149.30258
BB93659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24608	WGS84	BB93659	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93659_Mgig_Bot2012_COI	YD10	1	BB93659_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93659	Sample	BB93659	targeted	Absent	Sample	Mgig_Bot2012_COI	YD10	COI	45	BB93659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD10	TCTTATTCGTTGGAGACTTTATAACCCT	149.30159
BB93661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2443	WGS84	BB93661	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93661_Aamu_Bax2006_COI	YD7	1	BB93661_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93661	Sample	BB93661	targeted	Absent	Sample	Aamu_Bax2006_COI	YD7	COI	45	BB93661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD7	GCACAACCGGGATCTTTACTTC	149.30093
BB93661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2443	WGS84	BB93661	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93661_Pcan_Bot2011_IGS	YD7	1	BB93661_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93661	Sample	BB93661	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD7	IGS	45	BB93661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD7	CGTAATCCTCAGTACTGGCTA	149.30093
BB93661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2443	WGS84	BB93661	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93661_Sspa_Oph2007_COI	YD7	1	BB93661_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93661	Sample	BB93661	targeted	Absent	Sample	Sspa_Oph2007_COI	YD7	COI	45	BB93661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD7	GAAGGCCTTCCGATGATC	149.30093
BB93661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2443	WGS84	BB93661	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93661_Upin_Bot2011_COI	YD7	1	BB93661_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93661	Sample	BB93661	targeted	Absent	Sample	Upin_Bot2011_COI	YD7	COI	45	BB93661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD7	CTTTAATTACAGCGTTTTTATTGTTGT	149.30093
BB93662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2424	WGS84	BB93662	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93662_Mgig_Bot2012_COI	YD5	1	BB93662_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93662	Sample	BB93662	targeted	Absent	Sample	Mgig_Bot2012_COI	YD5	COI	45	BB93662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD5	TCTTATTCGTTGGAGACTTTATAACCCT	149.30257
BB93663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24239	WGS84	BB93663	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93663_Cmae_Bot2011_COI	YD4	1	BB93663_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93663	Sample	BB93663	targeted	Absent	Sample	Cmae_Bot2011_COI	YD4	COI	45	BB93663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD4	ATGAACAGTCTATCCTCCTTTAG	149.30341
BB93663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24239	WGS84	BB93663	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93663_Msal_Bot2012_COI	YD4	1	BB93663_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93663	Sample	BB93663	targeted	Absent	Sample	Msal_Bot2012_COI	YD4	COI	45	BB93663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD4	GYTAGTTCCRATGATGTTAGCTG	149.30341
BB93665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24181	WGS84	BB93665	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93665_Aamu_Bax2006_COI	YD1	1	BB93665_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93665	Sample	BB93665	targeted	Absent	Sample	Aamu_Bax2006_COI	YD1	COI	45	BB93665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD1	GCACAACCGGGATCTTTACTTC	149.30164
BB93665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24181	WGS84	BB93665	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93665_Pcan_Bot2011_IGS	YD1	1	BB93665_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93665	Sample	BB93665	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD1	IGS	45	BB93665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD1	CGTAATCCTCAGTACTGGCTA	149.30164
BB93665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24181	WGS84	BB93665	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93665_Sspa_Oph2007_COI	YD1	1	BB93665_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93665	Sample	BB93665	targeted	Absent	Sample	Sspa_Oph2007_COI	YD1	COI	45	BB93665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD1	GAAGGCCTTCCGATGATC	149.30164
BB93665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24181	WGS84	BB93665	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93665_Upin_Bot2011_COI	YD1	1	BB93665_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93665	Sample	BB93665	targeted	Absent	Sample	Upin_Bot2011_COI	YD1	COI	45	BB93665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD1	CTTTAATTACAGCGTTTTTATTGTTGT	149.30164
BB93666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93666	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93666_Mgig_Bot2012_COI	DD10	1	BB93666_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93666	Sample	BB93666	targeted	Absent	Sample	Mgig_Bot2012_COI	DD10	COI	45	BB93666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD10	TCTTATTCGTTGGAGACTTTATAACCCT	146.3657
BB93667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18664	WGS84	BB93667	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93667_Cmae_Bot2011_COI	DD8	1	BB93667_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93667	Sample	BB93667	targeted	Absent	Sample	Cmae_Bot2011_COI	DD8	COI	45	BB93667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD8	ATGAACAGTCTATCCTCCTTTAG	146.36605
BB93667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18664	WGS84	BB93667	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93667_Msal_Bot2012_COI	DD8	1	BB93667_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93667	Sample	BB93667	targeted	Absent	Sample	Msal_Bot2012_COI	DD8	COI	45	BB93667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD8	GYTAGTTCCRATGATGTTAGCTG	146.36605
BB93669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.19181	WGS84	BB93669	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93669_Aamu_Bax2006_COI	DD1	1	BB93669_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93669	Sample	BB93669	targeted	Absent	Sample	Aamu_Bax2006_COI	DD1	COI	45	BB93669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD1	GCACAACCGGGATCTTTACTTC	146.36808
BB93669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.19181	WGS84	BB93669	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93669_Pcan_Bot2011_IGS	DD1	1	BB93669_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93669	Sample	BB93669	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD1	IGS	45	BB93669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD1	CGTAATCCTCAGTACTGGCTA	146.36808
BB93669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.19181	WGS84	BB93669	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93669_Sspa_Oph2007_COI	DD1	1	BB93669_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93669	Sample	BB93669	targeted	Absent	Sample	Sspa_Oph2007_COI	DD1	COI	45	BB93669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD1	GAAGGCCTTCCGATGATC	146.36808
BB93669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.19181	WGS84	BB93669	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93669_Upin_Bot2011_COI	DD1	1	BB93669_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93669	Sample	BB93669	targeted	Absent	Sample	Upin_Bot2011_COI	DD1	COI	45	BB93669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD1	CTTTAATTACAGCGTTTTTATTGTTGT	146.36808
BB93670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.1886	WGS84	BB93670	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93670_Cmae_Bot2011_COI	DD3	1	BB93670_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93670	Sample	BB93670	targeted	Absent	Sample	Cmae_Bot2011_COI	DD3	COI	45	BB93670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD3	ATGAACAGTCTATCCTCCTTTAG	146.36678
BB93670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.1886	WGS84	BB93670	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93670_Msal_Bot2012_COI	DD3	1	BB93670_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93670	Sample	BB93670	targeted	Absent	Sample	Msal_Bot2012_COI	DD3	COI	45	BB93670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD3	GYTAGTTCCRATGATGTTAGCTG	146.36678
BB93672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93672	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93672_Pcan_Bot2011_IGS	DD9	1	BB93672_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93672	Sample	BB93672	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD9	IGS	45	BB93672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD9	CGTAATCCTCAGTACTGGCTA	146.36416
BB93672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93672	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93672_Sspa_Oph2007_COI	DD9	1	BB93672_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93672	Sample	BB93672	targeted	Absent	Sample	Sspa_Oph2007_COI	DD9	COI	45	BB93672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD9	GAAGGCCTTCCGATGATC	146.36416
BB93672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93672	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93672_Upin_Bot2011_COI	DD9	1	BB93672_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93672	Sample	BB93672	targeted	Absent	Sample	Upin_Bot2011_COI	DD9	COI	45	BB93672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD9	CTTTAATTACAGCGTTTTTATTGTTGT	146.36416
BB93673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18359	WGS84	BB93673	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93673_Mgig_Bot2012_COI	DD13	1	BB93673_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93673	Sample	BB93673	targeted	Absent	Sample	Mgig_Bot2012_COI	DD13	COI	45	BB93673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD13	TCTTATTCGTTGGAGACTTTATAACCCT	146.36547
BB93674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18474	WGS84	BB93674	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93674_Cmae_Bot2011_COI	DD12	1	BB93674_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93674	Sample	BB93674	targeted	Absent	Sample	Cmae_Bot2011_COI	DD12	COI	45	BB93674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD12	ATGAACAGTCTATCCTCCTTTAG	146.36414
BB93674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18474	WGS84	BB93674	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93674_Msal_Bot2012_COI	DD12	1	BB93674_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93674	Sample	BB93674	targeted	Absent	Sample	Msal_Bot2012_COI	DD12	COI	45	BB93674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD12	GYTAGTTCCRATGATGTTAGCTG	146.36414
BB93676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18283	WGS84	BB93676	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93676_Aamu_Bax2006_COI	DD15	1	BB93676_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93676	Sample	BB93676	targeted	Absent	Sample	Aamu_Bax2006_COI	DD15	COI	45	BB93676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD15	GCACAACCGGGATCTTTACTTC	146.36596
BB93676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18283	WGS84	BB93676	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93676_Pcan_Bot2011_IGS	DD15	1	BB93676_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93676	Sample	BB93676	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD15	IGS	45	BB93676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD15	CGTAATCCTCAGTACTGGCTA	146.36596
BB93676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18283	WGS84	BB93676	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93676_Sspa_Oph2007_COI	DD15	1	BB93676_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93676	Sample	BB93676	targeted	Absent	Sample	Sspa_Oph2007_COI	DD15	COI	45	BB93676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD15	GAAGGCCTTCCGATGATC	146.36596
BB93676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18283	WGS84	BB93676	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93676_Upin_Bot2011_COI	DD15	1	BB93676_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93676	Sample	BB93676	targeted	Absent	Sample	Upin_Bot2011_COI	DD15	COI	45	BB93676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD15	CTTTAATTACAGCGTTTTTATTGTTGT	146.36596
BB93677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18174	WGS84	BB93677	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.114	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93677_Mgig_Bot2012_COI	DD16	1	BB93677_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93677	Sample	BB93677	targeted	Present	Sample	Mgig_Bot2012_COI	DD16	COI	45	BB93677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD16	TCTTATTCGTTGGAGACTTTATAACCCT	146.36395
BB93678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18188	WGS84	BB93678	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.265	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93678_Cmae_Bot2011_COI	DD18	1	BB93678_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93678	Sample	BB93678	targeted	Present	Sample	Cmae_Bot2011_COI	DD18	COI	45	BB93678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD18	ATGAACAGTCTATCCTCCTTTAG	146.36437
BB93678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18188	WGS84	BB93678	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93678_Msal_Bot2012_COI	DD18	1	BB93678_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93678	Sample	BB93678	targeted	Absent	Sample	Msal_Bot2012_COI	DD18	COI	45	BB93678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD18	GYTAGTTCCRATGATGTTAGCTG	146.36437
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20811	WGS84	BB93680	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93680_Asen_Wil2025_COI	VD5	1	BB93680_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Asen_Wil2025_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	GTTGTTGTTACAACACATGCTCTTTTG	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93680_Cjap_Sim2018_COI	VD5	1	BB93680_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Cjap_Sim2018_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93680_Esin_And2018_Cyb	VD5	1	BB93680_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Esin_And2018_Cyb	VD5	Cytb	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	ACCCCTCCTCATATCCAACCA	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93680_Mgig_Bot2012_COI	VD5	1	BB93680_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Mgig_Bot2012_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	TCTTATTCGTTGGAGACTTTATAACCCT	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93680_Mjap_Gib2022_COI	VD5	1	BB93680_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Mjap_Gib2022_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	AATTTAATTGTGACTAGGCATGGTCTG	136.67224
BB93681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8857	WGS84	BB93681	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93681_Cmae_Bot2011_COI	HD21	1	BB93681_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93681	Sample	BB93681	targeted	Absent	Sample	Cmae_Bot2011_COI	HD21	COI	45	BB93681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD21	ATGAACAGTCTATCCTCCTTTAG	147.33873
BB93681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8857	WGS84	BB93681	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93681_Msal_Bot2012_COI	HD21	1	BB93681_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93681	Sample	BB93681	targeted	Absent	Sample	Msal_Bot2012_COI	HD21	COI	45	BB93681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD21	GYTAGTTCCRATGATGTTAGCTG	147.33873
BB93683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84434	WGS84	BB93683	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.46	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93683_Aamu_Bax2006_COI	HD6	1	BB93683_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93683	Sample	BB93683	targeted	Present	Sample	Aamu_Bax2006_COI	HD6	COI	45	BB93683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD6	GCACAACCGGGATCTTTACTTC	147.32872
BB93683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84434	WGS84	BB93683	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93683_Pcan_Bot2011_IGS	HD6	1	BB93683_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93683	Sample	BB93683	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD6	IGS	45	BB93683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD6	CGTAATCCTCAGTACTGGCTA	147.32872
BB93683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84434	WGS84	BB93683	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93683_Sspa_Oph2007_COI	HD6	1	BB93683_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93683	Sample	BB93683	targeted	Absent	Sample	Sspa_Oph2007_COI	HD6	COI	45	BB93683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD6	GAAGGCCTTCCGATGATC	147.32872
BB93683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84434	WGS84	BB93683	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93683_Upin_Bot2011_COI	HD6	1	BB93683_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93683	Sample	BB93683	targeted	Absent	Sample	Upin_Bot2011_COI	HD6	COI	45	BB93683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD6	CTTTAATTACAGCGTTTTTATTGTTGT	147.32872
BB93684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84382	WGS84	BB93684	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93684_Mgig_Bot2012_COI	HD7	1	BB93684_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93684	Sample	BB93684	targeted	Absent	Sample	Mgig_Bot2012_COI	HD7	COI	45	BB93684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD7	TCTTATTCGTTGGAGACTTTATAACCCT	147.32637
BB93685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84362	WGS84	BB93685	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.148	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93685_Cmae_Bot2011_COI	HD8	1	BB93685_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93685	Sample	BB93685	targeted	Present	Sample	Cmae_Bot2011_COI	HD8	COI	45	BB93685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD8	ATGAACAGTCTATCCTCCTTTAG	147.32459
BB93685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84362	WGS84	BB93685	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93685_Msal_Bot2012_COI	HD8	1	BB93685_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93685	Sample	BB93685	targeted	Absent	Sample	Msal_Bot2012_COI	HD8	COI	45	BB93685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD8	GYTAGTTCCRATGATGTTAGCTG	147.32459
BB93687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87576	WGS84	BB93687	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.837	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93687_Aamu_Bax2006_COI	HD11	1	BB93687_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93687	Sample	BB93687	targeted	Present	Sample	Aamu_Bax2006_COI	HD11	COI	45	BB93687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD11	GCACAACCGGGATCTTTACTTC	147.33896
BB93687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87576	WGS84	BB93687	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93687_Pcan_Bot2011_IGS	HD11	1	BB93687_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93687	Sample	BB93687	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD11	IGS	45	BB93687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD11	CGTAATCCTCAGTACTGGCTA	147.33896
BB93687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87576	WGS84	BB93687	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93687_Sspa_Oph2007_COI	HD11	1	BB93687_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93687	Sample	BB93687	targeted	Absent	Sample	Sspa_Oph2007_COI	HD11	COI	45	BB93687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD11	GAAGGCCTTCCGATGATC	147.33896
BB93687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87576	WGS84	BB93687	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93687_Upin_Bot2011_COI	HD11	1	BB93687_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93687	Sample	BB93687	targeted	Absent	Sample	Upin_Bot2011_COI	HD11	COI	45	BB93687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33896
BB93688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8757	WGS84	BB93688	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93688_Mgig_Bot2012_COI	HD13	1	BB93688_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93688	Sample	BB93688	targeted	Absent	Sample	Mgig_Bot2012_COI	HD13	COI	45	BB93688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD13	TCTTATTCGTTGGAGACTTTATAACCCT	147.34073
BB93689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87848	WGS84	BB93689	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93689_Cmae_Bot2011_COI	HD14	1	BB93689_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93689	Sample	BB93689	targeted	Absent	Sample	Cmae_Bot2011_COI	HD14	COI	45	BB93689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD14	ATGAACAGTCTATCCTCCTTTAG	147.34167
BB93689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87848	WGS84	BB93689	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93689_Msal_Bot2012_COI	HD14	1	BB93689_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93689	Sample	BB93689	targeted	Absent	Sample	Msal_Bot2012_COI	HD14	COI	45	BB93689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD14	GYTAGTTCCRATGATGTTAGCTG	147.34167
BB93690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88172	WGS84	BB93690	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.689	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93690_Aamu_Bax2006_COI	HD16	1	BB93690_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93690	Sample	BB93690	targeted	Present	Sample	Aamu_Bax2006_COI	HD16	COI	45	BB93690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD16	GCACAACCGGGATCTTTACTTC	147.34203
BB93690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88172	WGS84	BB93690	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93690_Pcan_Bot2011_IGS	HD16	1	BB93690_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93690	Sample	BB93690	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD16	IGS	45	BB93690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD16	CGTAATCCTCAGTACTGGCTA	147.34203
BB93690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88172	WGS84	BB93690	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93690_Sspa_Oph2007_COI	HD16	1	BB93690_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93690	Sample	BB93690	targeted	Absent	Sample	Sspa_Oph2007_COI	HD16	COI	45	BB93690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD16	GAAGGCCTTCCGATGATC	147.34203
BB93690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88172	WGS84	BB93690	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93690_Upin_Bot2011_COI	HD16	1	BB93690_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93690	Sample	BB93690	targeted	Absent	Sample	Upin_Bot2011_COI	HD16	COI	45	BB93690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD16	CTTTAATTACAGCGTTTTTATTGTTGT	147.34203
BB93691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88517	WGS84	BB93691	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.806	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93691_Mgig_Bot2012_COI	HD17	1	BB93691_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93691	Sample	BB93691	targeted	Present	Sample	Mgig_Bot2012_COI	HD17	COI	45	BB93691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD17	TCTTATTCGTTGGAGACTTTATAACCCT	147.33462
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93692_Cmae_Bot2011_COI	VD20	1	BB93692_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Cmae_Bot2011_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	ATGAACAGTCTATCCTCCTTTAG	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93692_Hsan_Wil2021_COI	VD20	1	BB93692_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Hsan_Wil2021_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	CCTGGGCCGGTATAGTAGGT	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93692_Mros_Gib2022_COI	VD20	1	BB93692_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Mros_Gib2022_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93692_Msal_Bot2012_COI	VD20	1	BB93692_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Msal_Bot2012_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	GYTAGTTCCRATGATGTTAGCTG	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93692_Pvir_Dia2013_COI	VD20	1	BB93692_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Pvir_Dia2013_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	CTTAGTGGCATTAATTCGDAATCC	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93692_Rhar_Sim2018_COI	VD20	1	BB93692_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Rhar_Sim2018_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	CTGCTGCTATTGCTCATG	136.68229
BB93694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19539	WGS84	BB93694	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93694_Aamu_Bax2006_COI	VD1	1	BB93694_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93694	Sample	BB93694	targeted	Absent	Sample	Aamu_Bax2006_COI	VD1	COI	45	BB93694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD1	GCACAACCGGGATCTTTACTTC	136.68657
BB93694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19539	WGS84	BB93694	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93694_Pcan_Bot2011_IGS	VD1	1	BB93694_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93694	Sample	BB93694	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD1	IGS	45	BB93694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD1	CGTAATCCTCAGTACTGGCTA	136.68657
BB93694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19539	WGS84	BB93694	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93694_Sspa_Oph2007_COI	VD1	1	BB93694_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93694	Sample	BB93694	targeted	Absent	Sample	Sspa_Oph2007_COI	VD1	COI	45	BB93694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD1	GAAGGCCTTCCGATGATC	136.68657
BB93694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19539	WGS84	BB93694	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93694_Upin_Bot2011_COI	VD1	1	BB93694_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93694	Sample	BB93694	targeted	Absent	Sample	Upin_Bot2011_COI	VD1	COI	45	BB93694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD1	CTTTAATTACAGCGTTTTTATTGTTGT	136.68657
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.19543	WGS84	BB93695	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93695_Asen_Wil2025_COI	VD2	1	BB93695_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Asen_Wil2025_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	GTTGTTGTTACAACACATGCTCTTTTG	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93695_Cjap_Sim2018_COI	VD2	1	BB93695_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Cjap_Sim2018_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93695_Esin_And2018_Cyb	VD2	1	BB93695_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Esin_And2018_Cyb	VD2	Cytb	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	ACCCCTCCTCATATCCAACCA	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93695_Mgig_Bot2012_COI	VD2	1	BB93695_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Mgig_Bot2012_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	TCTTATTCGTTGGAGACTTTATAACCCT	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93695_Mjap_Gib2022_COI	VD2	1	BB93695_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Mjap_Gib2022_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	AATTTAATTGTGACTAGGCATGGTCTG	136.68489
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93696_Cmae_Bot2011_COI	VD3	1	BB93696_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Cmae_Bot2011_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	ATGAACAGTCTATCCTCCTTTAG	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93696_Hsan_Wil2021_COI	VD3	1	BB93696_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Hsan_Wil2021_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	CCTGGGCCGGTATAGTAGGT	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93696_Msal_Bot2012_COI	VD3	1	BB93696_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Msal_Bot2012_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	GYTAGTTCCRATGATGTTAGCTG	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93696_Pvir_Dia2013_COI	VD3	1	BB93696_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Pvir_Dia2013_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	CTTAGTGGCATTAATTCGDAATCC	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93696_Rhar_Sim2018_COI	VD3	1	BB93696_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Rhar_Sim2018_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	CTGCTGCTATTGCTCATG	136.68091
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93698_Aamu_Bax2006_COI	VD18	1	BB93698_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Aamu_Bax2006_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	GCACAACCGGGATCTTTACTTC	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93698_Pcan_Bot2011_IGS	VD18	1	BB93698_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD18	IGS	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	CGTAATCCTCAGTACTGGCTA	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93698_Pper_Dia2013_COI	VD18	1	BB93698_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Pper_Dia2013_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	CTTAGTGGCATTAATTCGDAATCC	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93698_Sspa_Oph2007_COI	VD18	1	BB93698_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Sspa_Oph2007_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	GAAGGCCTTCCGATGATC	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93698_Upin_Bot2011_COI	VD18	1	BB93698_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Upin_Bot2011_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	CTTTAATTACAGCGTTTTTATTGTTGT	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20913	WGS84	BB93698	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93698_Vgib_Wil2025_COI	VD18	1	BB93698_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Vgib_Wil2025_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	CCCCTCTTTACAAGTTCTTTAGGGT	136.66947
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20703	WGS84	BB93699	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93699_Asen_Wil2025_COI	VD16	1	BB93699_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Asen_Wil2025_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	GTTGTTGTTACAACACATGCTCTTTTG	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93699_Cjap_Sim2018_COI	VD16	1	BB93699_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Cjap_Sim2018_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93699_Esin_And2018_Cyb	VD16	1	BB93699_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Esin_And2018_Cyb	VD16	Cytb	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	ACCCCTCCTCATATCCAACCA	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93699_Mgig_Bot2012_COI	VD16	1	BB93699_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Mgig_Bot2012_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	TCTTATTCGTTGGAGACTTTATAACCCT	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93699_Mjap_Gib2022_COI	VD16	1	BB93699_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Mjap_Gib2022_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	AATTTAATTGTGACTAGGCATGGTCTG	136.66813
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93700_Cmae_Bot2011_COI	VD14	1	BB93700_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Cmae_Bot2011_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	ATGAACAGTCTATCCTCCTTTAG	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93700_Hsan_Wil2021_COI	VD14	1	BB93700_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Hsan_Wil2021_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	CCTGGGCCGGTATAGTAGGT	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93700_Mros_Gib2022_COI	VD14	1	BB93700_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Mros_Gib2022_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93700_Msal_Bot2012_COI	VD14	1	BB93700_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Msal_Bot2012_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	GYTAGTTCCRATGATGTTAGCTG	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93700_Pvir_Dia2013_COI	VD14	1	BB93700_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Pvir_Dia2013_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	CTTAGTGGCATTAATTCGDAATCC	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93700_Rhar_Sim2018_COI	VD14	1	BB93700_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Rhar_Sim2018_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	CTGCTGCTATTGCTCATG	136.67151
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93702_Aamu_Bax2006_COI	VD12	1	BB93702_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Aamu_Bax2006_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	GCACAACCGGGATCTTTACTTC	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93702_Pcan_Bot2011_IGS	VD12	1	BB93702_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD12	IGS	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	CGTAATCCTCAGTACTGGCTA	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93702_Pper_Dia2013_COI	VD12	1	BB93702_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Pper_Dia2013_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	CTTAGTGGCATTAATTCGDAATCC	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93702_Sspa_Oph2007_COI	VD12	1	BB93702_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Sspa_Oph2007_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	GAAGGCCTTCCGATGATC	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93702_Upin_Bot2011_COI	VD12	1	BB93702_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Upin_Bot2011_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	CTTTAATTACAGCGTTTTTATTGTTGT	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20771	WGS84	BB93702	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93702_Vgib_Wil2025_COI	VD12	1	BB93702_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Vgib_Wil2025_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	CCCCTCTTTACAAGTTCTTTAGGGT	136.6698
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20367	WGS84	BB93703	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93703_Asen_Wil2025_COI	VD8	1	BB93703_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Asen_Wil2025_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	GTTGTTGTTACAACACATGCTCTTTTG	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93703_Cjap_Sim2018_COI	VD8	1	BB93703_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Cjap_Sim2018_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93703_Esin_And2018_Cyb	VD8	1	BB93703_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Esin_And2018_Cyb	VD8	Cytb	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	ACCCCTCCTCATATCCAACCA	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93703_Mgig_Bot2012_COI	VD8	1	BB93703_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Mgig_Bot2012_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	TCTTATTCGTTGGAGACTTTATAACCCT	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93703_Mjap_Gib2022_COI	VD8	1	BB93703_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Mjap_Gib2022_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	AATTTAATTGTGACTAGGCATGGTCTG	136.6702
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93704_Cmae_Bot2011_COI	VD7	1	BB93704_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Cmae_Bot2011_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	ATGAACAGTCTATCCTCCTTTAG	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93704_Hsan_Wil2021_COI	VD7	1	BB93704_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Hsan_Wil2021_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	CCTGGGCCGGTATAGTAGGT	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93704_Mros_Gib2022_COI	VD7	1	BB93704_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Mros_Gib2022_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93704_Msal_Bot2012_COI	VD7	1	BB93704_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Msal_Bot2012_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	GYTAGTTCCRATGATGTTAGCTG	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93704_Pvir_Dia2013_COI	VD7	1	BB93704_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Pvir_Dia2013_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	CTTAGTGGCATTAATTCGDAATCC	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93704_Rhar_Sim2018_COI	VD7	1	BB93704_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Rhar_Sim2018_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	CTGCTGCTATTGCTCATG	136.67046
BB93706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84552	WGS84	BB93706	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93706_Aamu_Bax2006_COI	HD5	1	BB93706_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93706	Sample	BB93706	targeted	Absent	Sample	Aamu_Bax2006_COI	HD5	COI	45	BB93706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD5	GCACAACCGGGATCTTTACTTC	147.33115
BB93706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84552	WGS84	BB93706	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93706_Pcan_Bot2011_IGS	HD5	1	BB93706_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93706	Sample	BB93706	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD5	IGS	45	BB93706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD5	CGTAATCCTCAGTACTGGCTA	147.33115
BB93706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84552	WGS84	BB93706	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93706_Sspa_Oph2007_COI	HD5	1	BB93706_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93706	Sample	BB93706	targeted	Absent	Sample	Sspa_Oph2007_COI	HD5	COI	45	BB93706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD5	GAAGGCCTTCCGATGATC	147.33115
BB93706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84552	WGS84	BB93706	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93706_Upin_Bot2011_COI	HD5	1	BB93706_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93706	Sample	BB93706	targeted	Absent	Sample	Upin_Bot2011_COI	HD5	COI	45	BB93706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD5	CTTTAATTACAGCGTTTTTATTGTTGT	147.33115
BB93707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84389	WGS84	BB93707	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93707_Mgig_Bot2012_COI	HD4	1	BB93707_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93707	Sample	BB93707	targeted	Absent	Sample	Mgig_Bot2012_COI	HD4	COI	45	BB93707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD4	TCTTATTCGTTGGAGACTTTATAACCCT	147.33123
BB93708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82814	WGS84	BB93708	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.566	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93708_Cmae_Bot2011_COI	HD3	1	BB93708_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93708	Sample	BB93708	targeted	Present	Sample	Cmae_Bot2011_COI	HD3	COI	45	BB93708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD3	ATGAACAGTCTATCCTCCTTTAG	147.31543
BB93708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82814	WGS84	BB93708	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93708_Msal_Bot2012_COI	HD3	1	BB93708_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93708	Sample	BB93708	targeted	Absent	Sample	Msal_Bot2012_COI	HD3	COI	45	BB93708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD3	GYTAGTTCCRATGATGTTAGCTG	147.31543
BB93710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67457	WGS84	BB93710	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93710_Mgig_Bot2012_COI	WD15	1	BB93710_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93710	Sample	BB93710	targeted	Absent	Sample	Mgig_Bot2012_COI	WD15	COI	45	BB93710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD15	TCTTATTCGTTGGAGACTTTATAACCCT	141.87024
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93711_Cmae_Bot2011_COI	WD1	1	BB93711_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Cmae_Bot2011_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	ATGAACAGTCTATCCTCCTTTAG	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93711_Hsan_Wil2021_COI	WD1	1	BB93711_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Hsan_Wil2021_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	CCTGGGCCGGTATAGTAGGT	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93711_Mros_Gib2022_COI	WD1	1	BB93711_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Mros_Gib2022_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93711_Msal_Bot2012_COI	WD1	1	BB93711_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Msal_Bot2012_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	GYTAGTTCCRATGATGTTAGCTG	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93711_Pvir_Dia2013_COI	WD1	1	BB93711_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Pvir_Dia2013_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	CTTAGTGGCATTAATTCGDAATCC	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93711_Rhar_Sim2018_COI	WD1	1	BB93711_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Rhar_Sim2018_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	CTGCTGCTATTGCTCATG	141.84674
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93713_Aamu_Bax2006_COI	WD4	1	BB93713_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Aamu_Bax2006_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	GCACAACCGGGATCTTTACTTC	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93713_Pcan_Bot2011_IGS	WD4	1	BB93713_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD4	IGS	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	CGTAATCCTCAGTACTGGCTA	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93713_Pper_Dia2013_COI	WD4	1	BB93713_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Pper_Dia2013_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	CTTAGTGGCATTAATTCGDAATCC	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93713_Sspa_Oph2007_COI	WD4	1	BB93713_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Sspa_Oph2007_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	GAAGGCCTTCCGATGATC	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93713_Upin_Bot2011_COI	WD4	1	BB93713_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Upin_Bot2011_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	CTTTAATTACAGCGTTTTTATTGTTGT	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66603	WGS84	BB93713	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93713_Vgib_Wil2025_COI	WD4	1	BB93713_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Vgib_Wil2025_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	CCCCTCTTTACAAGTTCTTTAGGGT	141.84863
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66878	WGS84	BB93714	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93714_Asen_Wil2025_COI	WD7	1	BB93714_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Asen_Wil2025_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	GTTGTTGTTACAACACATGCTCTTTTG	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93714_Cjap_Sim2018_COI	WD7	1	BB93714_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Cjap_Sim2018_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93714_Esin_And2018_Cyb	WD7	1	BB93714_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Esin_And2018_Cyb	WD7	Cytb	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	ACCCCTCCTCATATCCAACCA	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93714_Mgig_Bot2012_COI	WD7	1	BB93714_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Mgig_Bot2012_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	TCTTATTCGTTGGAGACTTTATAACCCT	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93714_Mjap_Gib2022_COI	WD7	1	BB93714_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Mjap_Gib2022_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	AATTTAATTGTGACTAGGCATGGTCTG	141.86098
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93715_Cmae_Bot2011_COI	WD8	1	BB93715_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Cmae_Bot2011_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	ATGAACAGTCTATCCTCCTTTAG	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93715_Hsan_Wil2021_COI	WD8	1	BB93715_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Hsan_Wil2021_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	CCTGGGCCGGTATAGTAGGT	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93715_Mros_Gib2022_COI	WD8	1	BB93715_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Mros_Gib2022_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93715_Msal_Bot2012_COI	WD8	1	BB93715_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Msal_Bot2012_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	GYTAGTTCCRATGATGTTAGCTG	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93715_Pvir_Dia2013_COI	WD8	1	BB93715_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Pvir_Dia2013_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	CTTAGTGGCATTAATTCGDAATCC	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93715_Rhar_Sim2018_COI	WD8	1	BB93715_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Rhar_Sim2018_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	CTGCTGCTATTGCTCATG	141.86241
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93717_Aamu_Bax2006_COI	WD11	1	BB93717_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Aamu_Bax2006_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	GCACAACCGGGATCTTTACTTC	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93717_Pcan_Bot2011_IGS	WD11	1	BB93717_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD11	IGS	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	CGTAATCCTCAGTACTGGCTA	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93717_Pper_Dia2013_COI	WD11	1	BB93717_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Pper_Dia2013_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	CTTAGTGGCATTAATTCGDAATCC	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93717_Sspa_Oph2007_COI	WD11	1	BB93717_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Sspa_Oph2007_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	GAAGGCCTTCCGATGATC	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93717_Upin_Bot2011_COI	WD11	1	BB93717_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Upin_Bot2011_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	CTTTAATTACAGCGTTTTTATTGTTGT	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67253	WGS84	BB93717	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93717_Vgib_Wil2025_COI	WD11	1	BB93717_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Vgib_Wil2025_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	CCCCTCTTTACAAGTTCTTTAGGGT	141.86743
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67373	WGS84	BB93718	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93718_Asen_Wil2025_COI	WD12	1	BB93718_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Asen_Wil2025_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	GTTGTTGTTACAACACATGCTCTTTTG	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93718_Cjap_Sim2018_COI	WD12	1	BB93718_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Cjap_Sim2018_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93718_Esin_And2018_Cyb	WD12	1	BB93718_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Esin_And2018_Cyb	WD12	Cytb	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	ACCCCTCCTCATATCCAACCA	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93718_Mgig_Bot2012_COI	WD12	1	BB93718_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Mgig_Bot2012_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	TCTTATTCGTTGGAGACTTTATAACCCT	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93718_Mjap_Gib2022_COI	WD12	1	BB93718_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Mjap_Gib2022_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	AATTTAATTGTGACTAGGCATGGTCTG	141.86683
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93719_Cmae_Bot2011_COI	WD14	1	BB93719_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Cmae_Bot2011_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	ATGAACAGTCTATCCTCCTTTAG	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93719_Hsan_Wil2021_COI	WD14	1	BB93719_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Hsan_Wil2021_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	CCTGGGCCGGTATAGTAGGT	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93719_Mros_Gib2022_COI	WD14	1	BB93719_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Mros_Gib2022_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93719_Msal_Bot2012_COI	WD14	1	BB93719_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Msal_Bot2012_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	GYTAGTTCCRATGATGTTAGCTG	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93719_Pvir_Dia2013_COI	WD14	1	BB93719_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Pvir_Dia2013_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	CTTAGTGGCATTAATTCGDAATCC	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93719_Rhar_Sim2018_COI	WD14	1	BB93719_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Rhar_Sim2018_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	CTGCTGCTATTGCTCATG	141.86939
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93720_Aamu_Bax2006_COI	WD16	1	BB93720_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Aamu_Bax2006_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	GCACAACCGGGATCTTTACTTC	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93720_Pcan_Bot2011_IGS	WD16	1	BB93720_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD16	IGS	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	CGTAATCCTCAGTACTGGCTA	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93720_Pper_Dia2013_COI	WD16	1	BB93720_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Pper_Dia2013_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	CTTAGTGGCATTAATTCGDAATCC	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93720_Sspa_Oph2007_COI	WD16	1	BB93720_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Sspa_Oph2007_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	GAAGGCCTTCCGATGATC	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93720_Upin_Bot2011_COI	WD16	1	BB93720_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Upin_Bot2011_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	CTTTAATTACAGCGTTTTTATTGTTGT	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67469	WGS84	BB93720	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93720_Vgib_Wil2025_COI	WD16	1	BB93720_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Vgib_Wil2025_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	CCCCTCTTTACAAGTTCTTTAGGGT	141.86884
BB93721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67454	WGS84	BB93721	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93721_Mgig_Bot2012_COI	WD18	1	BB93721_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93721	Sample	BB93721	targeted	Absent	Sample	Mgig_Bot2012_COI	WD18	COI	45	BB93721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD18	TCTTATTCGTTGGAGACTTTATAACCCT	141.87152
BB93722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67312	WGS84	BB93722	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93722_Cmae_Bot2011_COI	WD19	1	BB93722_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93722	Sample	BB93722	targeted	Absent	Sample	Cmae_Bot2011_COI	WD19	COI	45	BB93722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD19	ATGAACAGTCTATCCTCCTTTAG	141.87125
BB93722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67312	WGS84	BB93722	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93722_Msal_Bot2012_COI	WD19	1	BB93722_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93722	Sample	BB93722	targeted	Absent	Sample	Msal_Bot2012_COI	WD19	COI	45	BB93722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD19	GYTAGTTCCRATGATGTTAGCTG	141.87125
BB93724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67261	WGS84	BB93724	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93724_Aamu_Bax2006_COI	WD10	1	BB93724_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93724	Sample	BB93724	targeted	Absent	Sample	Aamu_Bax2006_COI	WD10	COI	45	BB93724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD10	GCACAACCGGGATCTTTACTTC	141.86627
BB93724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67261	WGS84	BB93724	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93724_Pcan_Bot2011_IGS	WD10	1	BB93724_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93724	Sample	BB93724	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD10	IGS	45	BB93724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD10	CGTAATCCTCAGTACTGGCTA	141.86627
BB93724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67261	WGS84	BB93724	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93724_Sspa_Oph2007_COI	WD10	1	BB93724_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93724	Sample	BB93724	targeted	Absent	Sample	Sspa_Oph2007_COI	WD10	COI	45	BB93724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD10	GAAGGCCTTCCGATGATC	141.86627
BB93724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67261	WGS84	BB93724	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93724_Upin_Bot2011_COI	WD10	1	BB93724_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93724	Sample	BB93724	targeted	Absent	Sample	Upin_Bot2011_COI	WD10	COI	45	BB93724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD10	CTTTAATTACAGCGTTTTTATTGTTGT	141.86627
BB93725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81853	WGS84	BB93725	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.362	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93725_Mgig_Bot2012_COI	MC75	1	BB93725_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93725	Sample	BB93725	targeted	Present	Sample	Mgig_Bot2012_COI	MC75	COI	45	BB93725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC75	TCTTATTCGTTGGAGACTTTATAACCCT	144.92784
BB93726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81884	WGS84	BB93726	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93726_Cmae_Bot2011_COI	MC70	1	BB93726_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93726	Sample	BB93726	targeted	Absent	Sample	Cmae_Bot2011_COI	MC70	COI	45	BB93726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC70	ATGAACAGTCTATCCTCCTTTAG	144.92457
BB93726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81884	WGS84	BB93726	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93726_Msal_Bot2012_COI	MC70	1	BB93726_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93726	Sample	BB93726	targeted	Absent	Sample	Msal_Bot2012_COI	MC70	COI	45	BB93726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC70	GYTAGTTCCRATGATGTTAGCTG	144.92457
BB93728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86071	WGS84	BB93728	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93728_Aamu_Bax2006_COI	MC45	1	BB93728_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93728	Sample	BB93728	targeted	Absent	Sample	Aamu_Bax2006_COI	MC45	COI	45	BB93728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC45	GCACAACCGGGATCTTTACTTC	144.9137
BB93728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86071	WGS84	BB93728	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93728_Pcan_Bot2011_IGS	MC45	1	BB93728_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93728	Sample	BB93728	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC45	IGS	45	BB93728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC45	CGTAATCCTCAGTACTGGCTA	144.9137
BB93728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86071	WGS84	BB93728	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.144	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93728_Sspa_Oph2007_COI	MC45	1	BB93728_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93728	Sample	BB93728	targeted	Present	Sample	Sspa_Oph2007_COI	MC45	COI	45	BB93728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC45	GAAGGCCTTCCGATGATC	144.9137
BB93728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86071	WGS84	BB93728	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93728_Upin_Bot2011_COI	MC45	1	BB93728_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93728	Sample	BB93728	targeted	Absent	Sample	Upin_Bot2011_COI	MC45	COI	45	BB93728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC45	CTTTAATTACAGCGTTTTTATTGTTGT	144.9137
BB93729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86215	WGS84	BB93729	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.875	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93729_Mgig_Bot2012_COI	MC40	1	BB93729_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93729	Sample	BB93729	targeted	Present	Sample	Mgig_Bot2012_COI	MC40	COI	45	BB93729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC40	TCTTATTCGTTGGAGACTTTATAACCCT	144.91262
BB93731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8489	WGS84	BB93731	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93731_Aamu_Bax2006_COI	MC15	1	BB93731_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93731	Sample	BB93731	targeted	Absent	Sample	Aamu_Bax2006_COI	MC15	COI	45	BB93731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC15	GCACAACCGGGATCTTTACTTC	144.91028
BB93731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8489	WGS84	BB93731	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93731_Pcan_Bot2011_IGS	MC15	1	BB93731_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93731	Sample	BB93731	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC15	IGS	45	BB93731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC15	CGTAATCCTCAGTACTGGCTA	144.91028
BB93731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8489	WGS84	BB93731	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.611	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93731_Sspa_Oph2007_COI	MC15	1	BB93731_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93731	Sample	BB93731	targeted	Present	Sample	Sspa_Oph2007_COI	MC15	COI	45	BB93731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC15	GAAGGCCTTCCGATGATC	144.91028
BB93731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8489	WGS84	BB93731	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93731_Upin_Bot2011_COI	MC15	1	BB93731_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93731	Sample	BB93731	targeted	Absent	Sample	Upin_Bot2011_COI	MC15	COI	45	BB93731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC15	CTTTAATTACAGCGTTTTTATTGTTGT	144.91028
BB93732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81862	WGS84	BB93732	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.874	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93732_Mgig_Bot2012_COI	MC85	1	BB93732_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93732	Sample	BB93732	targeted	Present	Sample	Mgig_Bot2012_COI	MC85	COI	45	BB93732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC85	TCTTATTCGTTGGAGACTTTATAACCCT	144.92195
BB93733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84169	WGS84	BB93733	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93733_Cmae_Bot2011_COI	MC5	1	BB93733_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93733	Sample	BB93733	targeted	Absent	Sample	Cmae_Bot2011_COI	MC5	COI	45	BB93733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC5	ATGAACAGTCTATCCTCCTTTAG	144.92949
BB93733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84169	WGS84	BB93733	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93733_Msal_Bot2012_COI	MC5	1	BB93733_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93733	Sample	BB93733	targeted	Absent	Sample	Msal_Bot2012_COI	MC5	COI	45	BB93733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC5	GYTAGTTCCRATGATGTTAGCTG	144.92949
BB93735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93735	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93735_Aamu_Bax2006_COI	MC110	1	BB93735_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93735	Sample	BB93735	targeted	Absent	Sample	Aamu_Bax2006_COI	MC110	COI	45	BB93735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC110	GCACAACCGGGATCTTTACTTC	144.91218
BB93735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93735	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93735_Pcan_Bot2011_IGS	MC110	1	BB93735_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93735	Sample	BB93735	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC110	IGS	45	BB93735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC110	CGTAATCCTCAGTACTGGCTA	144.91218
BB93735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93735	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.293	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93735_Sspa_Oph2007_COI	MC110	1	BB93735_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93735	Sample	BB93735	targeted	Present	Sample	Sspa_Oph2007_COI	MC110	COI	45	BB93735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC110	GAAGGCCTTCCGATGATC	144.91218
BB93735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93735	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93735_Upin_Bot2011_COI	MC110	1	BB93735_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93735	Sample	BB93735	targeted	Absent	Sample	Upin_Bot2011_COI	MC110	COI	45	BB93735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC110	CTTTAATTACAGCGTTTTTATTGTTGT	144.91218
BB93736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81673	WGS84	BB93736	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.889	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93736_Mgig_Bot2012_COI	MC78	1	BB93736_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93736	Sample	BB93736	targeted	Present	Sample	Mgig_Bot2012_COI	MC78	COI	45	BB93736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC78	TCTTATTCGTTGGAGACTTTATAACCCT	144.92566
BB93737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81959	WGS84	BB93737	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93737_Cmae_Bot2011_COI	MC77	1	BB93737_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93737	Sample	BB93737	targeted	Absent	Sample	Cmae_Bot2011_COI	MC77	COI	45	BB93737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC77	ATGAACAGTCTATCCTCCTTTAG	144.92662
BB93737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81959	WGS84	BB93737	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93737_Msal_Bot2012_COI	MC77	1	BB93737_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93737	Sample	BB93737	targeted	Absent	Sample	Msal_Bot2012_COI	MC77	COI	45	BB93737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC77	GYTAGTTCCRATGATGTTAGCTG	144.92662
BB93739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82006	WGS84	BB93739	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93739_Aamu_Bax2006_COI	MC72	1	BB93739_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93739	Sample	BB93739	targeted	Absent	Sample	Aamu_Bax2006_COI	MC72	COI	45	BB93739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC72	GCACAACCGGGATCTTTACTTC	144.93236
BB93739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82006	WGS84	BB93739	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93739_Pcan_Bot2011_IGS	MC72	1	BB93739_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93739	Sample	BB93739	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC72	IGS	45	BB93739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC72	CGTAATCCTCAGTACTGGCTA	144.93236
BB93739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82006	WGS84	BB93739	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.299	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93739_Sspa_Oph2007_COI	MC72	1	BB93739_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93739	Sample	BB93739	targeted	Present	Sample	Sspa_Oph2007_COI	MC72	COI	45	BB93739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC72	GAAGGCCTTCCGATGATC	144.93236
BB93739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82006	WGS84	BB93739	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93739_Upin_Bot2011_COI	MC72	1	BB93739_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93739	Sample	BB93739	targeted	Absent	Sample	Upin_Bot2011_COI	MC72	COI	45	BB93739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC72	CTTTAATTACAGCGTTTTTATTGTTGT	144.93236
BB93740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81812	WGS84	BB93740	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93740_Cmae_Bot2011_COI	MC68	1	BB93740_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93740	Sample	BB93740	targeted	Absent	Sample	Cmae_Bot2011_COI	MC68	COI	45	BB93740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC68	ATGAACAGTCTATCCTCCTTTAG	144.9219
BB93740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81812	WGS84	BB93740	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93740_Msal_Bot2012_COI	MC68	1	BB93740_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93740	Sample	BB93740	targeted	Absent	Sample	Msal_Bot2012_COI	MC68	COI	45	BB93740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC68	GYTAGTTCCRATGATGTTAGCTG	144.9219
BB93742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81982	WGS84	BB93742	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93742_Aamu_Bax2006_COI	MC64	1	BB93742_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93742	Sample	BB93742	targeted	Absent	Sample	Aamu_Bax2006_COI	MC64	COI	45	BB93742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC64	GCACAACCGGGATCTTTACTTC	144.91739
BB93742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81982	WGS84	BB93742	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93742_Pcan_Bot2011_IGS	MC64	1	BB93742_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93742	Sample	BB93742	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC64	IGS	45	BB93742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC64	CGTAATCCTCAGTACTGGCTA	144.91739
BB93742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81982	WGS84	BB93742	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.59	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93742_Sspa_Oph2007_COI	MC64	1	BB93742_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93742	Sample	BB93742	targeted	Present	Sample	Sspa_Oph2007_COI	MC64	COI	45	BB93742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC64	GAAGGCCTTCCGATGATC	144.91739
BB93742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81982	WGS84	BB93742	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93742_Upin_Bot2011_COI	MC64	1	BB93742_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93742	Sample	BB93742	targeted	Absent	Sample	Upin_Bot2011_COI	MC64	COI	45	BB93742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC64	CTTTAATTACAGCGTTTTTATTGTTGT	144.91739
BB93743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82302	WGS84	BB93743	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.847	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93743_Mgig_Bot2012_COI	MC62	1	BB93743_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93743	Sample	BB93743	targeted	Present	Sample	Mgig_Bot2012_COI	MC62	COI	45	BB93743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC62	TCTTATTCGTTGGAGACTTTATAACCCT	144.90666
BB93744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82503	WGS84	BB93744	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93744_Cmae_Bot2011_COI	MC54	1	BB93744_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93744	Sample	BB93744	targeted	Absent	Sample	Cmae_Bot2011_COI	MC54	COI	45	BB93744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC54	ATGAACAGTCTATCCTCCTTTAG	144.9036
BB93744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82503	WGS84	BB93744	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93744_Msal_Bot2012_COI	MC54	1	BB93744_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93744	Sample	BB93744	targeted	Absent	Sample	Msal_Bot2012_COI	MC54	COI	45	BB93744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC54	GYTAGTTCCRATGATGTTAGCTG	144.9036
BB93746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82613	WGS84	BB93746	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93746_Aamu_Bax2006_COI	MC52	1	BB93746_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93746	Sample	BB93746	targeted	Absent	Sample	Aamu_Bax2006_COI	MC52	COI	45	BB93746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC52	GCACAACCGGGATCTTTACTTC	144.9012
BB93746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82613	WGS84	BB93746	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93746_Pcan_Bot2011_IGS	MC52	1	BB93746_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93746	Sample	BB93746	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC52	IGS	45	BB93746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC52	CGTAATCCTCAGTACTGGCTA	144.9012
BB93746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82613	WGS84	BB93746	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.525	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93746_Sspa_Oph2007_COI	MC52	1	BB93746_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93746	Sample	BB93746	targeted	Present	Sample	Sspa_Oph2007_COI	MC52	COI	45	BB93746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC52	GAAGGCCTTCCGATGATC	144.9012
BB93746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82613	WGS84	BB93746	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93746_Upin_Bot2011_COI	MC52	1	BB93746_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93746	Sample	BB93746	targeted	Absent	Sample	Upin_Bot2011_COI	MC52	COI	45	BB93746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC52	CTTTAATTACAGCGTTTTTATTGTTGT	144.9012
BB93747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8609	WGS84	BB93747	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.802	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93747_Mgig_Bot2012_COI	MC48	1	BB93747_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93747	Sample	BB93747	targeted	Present	Sample	Mgig_Bot2012_COI	MC48	COI	45	BB93747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC48	TCTTATTCGTTGGAGACTTTATAACCCT	144.91203
BB93748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85994	WGS84	BB93748	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93748_Cmae_Bot2011_COI	MC47	1	BB93748_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93748	Sample	BB93748	targeted	Absent	Sample	Cmae_Bot2011_COI	MC47	COI	45	BB93748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC47	ATGAACAGTCTATCCTCCTTTAG	144.91173
BB93748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85994	WGS84	BB93748	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93748_Msal_Bot2012_COI	MC47	1	BB93748_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93748	Sample	BB93748	targeted	Absent	Sample	Msal_Bot2012_COI	MC47	COI	45	BB93748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC47	GYTAGTTCCRATGATGTTAGCTG	144.91173
BB93750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86157	WGS84	BB93750	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.297	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93750_Mgig_Bot2012_COI	MC44	1	BB93750_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93750	Sample	BB93750	targeted	Present	Sample	Mgig_Bot2012_COI	MC44	COI	45	BB93750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC44	TCTTATTCGTTGGAGACTTTATAACCCT	144.91759
BB93751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93751	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93751_Cmae_Bot2011_COI	MC43	1	BB93751_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93751	Sample	BB93751	targeted	Absent	Sample	Cmae_Bot2011_COI	MC43	COI	45	BB93751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC43	ATGAACAGTCTATCCTCCTTTAG	144.91536
BB93751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93751	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93751_Msal_Bot2012_COI	MC43	1	BB93751_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93751	Sample	BB93751	targeted	Absent	Sample	Msal_Bot2012_COI	MC43	COI	45	BB93751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC43	GYTAGTTCCRATGATGTTAGCTG	144.91536
BB93753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93753	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93753_Aamu_Bax2006_COI	MC41	1	BB93753_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93753	Sample	BB93753	targeted	Absent	Sample	Aamu_Bax2006_COI	MC41	COI	45	BB93753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC41	GCACAACCGGGATCTTTACTTC	144.91334
BB93753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93753	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93753_Pcan_Bot2011_IGS	MC41	1	BB93753_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93753	Sample	BB93753	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC41	IGS	45	BB93753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC41	CGTAATCCTCAGTACTGGCTA	144.91334
BB93753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93753	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.772	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93753_Sspa_Oph2007_COI	MC41	1	BB93753_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93753	Sample	BB93753	targeted	Present	Sample	Sspa_Oph2007_COI	MC41	COI	45	BB93753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC41	GAAGGCCTTCCGATGATC	144.91334
BB93753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93753	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93753_Upin_Bot2011_COI	MC41	1	BB93753_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93753	Sample	BB93753	targeted	Absent	Sample	Upin_Bot2011_COI	MC41	COI	45	BB93753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC41	CTTTAATTACAGCGTTTTTATTGTTGT	144.91334
BB93754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86172	WGS84	BB93754	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.099	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93754_Mgig_Bot2012_COI	MC39	1	BB93754_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93754	Sample	BB93754	targeted	Present	Sample	Mgig_Bot2012_COI	MC39	COI	45	BB93754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC39	TCTTATTCGTTGGAGACTTTATAACCCT	144.91464
BB93755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86339	WGS84	BB93755	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93755_Cmae_Bot2011_COI	MC38	1	BB93755_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93755	Sample	BB93755	targeted	Absent	Sample	Cmae_Bot2011_COI	MC38	COI	45	BB93755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC38	ATGAACAGTCTATCCTCCTTTAG	144.91381
BB93755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86339	WGS84	BB93755	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93755_Msal_Bot2012_COI	MC38	1	BB93755_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93755	Sample	BB93755	targeted	Absent	Sample	Msal_Bot2012_COI	MC38	COI	45	BB93755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC38	GYTAGTTCCRATGATGTTAGCTG	144.91381
BB93757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86471	WGS84	BB93757	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.063	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93757_Aamu_Bax2006_COI	MC36	1	BB93757_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93757	Sample	BB93757	targeted	Present	Sample	Aamu_Bax2006_COI	MC36	COI	45	BB93757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC36	GCACAACCGGGATCTTTACTTC	144.91845
BB93757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86471	WGS84	BB93757	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93757_Pcan_Bot2011_IGS	MC36	1	BB93757_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93757	Sample	BB93757	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC36	IGS	45	BB93757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC36	CGTAATCCTCAGTACTGGCTA	144.91845
BB93757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86471	WGS84	BB93757	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93757_Sspa_Oph2007_COI	MC36	1	BB93757_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93757	Sample	BB93757	targeted	Absent	Sample	Sspa_Oph2007_COI	MC36	COI	45	BB93757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC36	GAAGGCCTTCCGATGATC	144.91845
BB93757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86471	WGS84	BB93757	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93757_Upin_Bot2011_COI	MC36	1	BB93757_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93757	Sample	BB93757	targeted	Absent	Sample	Upin_Bot2011_COI	MC36	COI	45	BB93757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC36	CTTTAATTACAGCGTTTTTATTGTTGT	144.91845
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.84	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93758_Mgig_Bot2012_COI	MC33	1	BB93758_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Present	Sample	Mgig_Bot2012_COI	MC33	COI	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	TCTTATTCGTTGGAGACTTTATAACCCT	144.91624
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93759_Cmae_Bot2011_COI	MC34	1	BB93759_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Absent	Sample	Cmae_Bot2011_COI	MC34	COI	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	ATGAACAGTCTATCCTCCTTTAG	144.91507
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93759_Hsan_Wil2021_COI	MC34	1	BB93759_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Absent	Sample	Hsan_Wil2021_COI	MC34	COI	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	CCTGGGCCGGTATAGTAGGT	144.91507
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93759_Msal_Bot2012_COI	MC34	1	BB93759_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Absent	Sample	Msal_Bot2012_COI	MC34	COI	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	GYTAGTTCCRATGATGTTAGCTG	144.91507
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93760_Aamu_Bax2006_COI	MC32	1	BB93760_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Absent	Sample	Aamu_Bax2006_COI	MC32	COI	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	GCACAACCGGGATCTTTACTTC	144.91742
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93760_Pcan_Bot2011_IGS	MC32	1	BB93760_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC32	IGS	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	CGTAATCCTCAGTACTGGCTA	144.91742
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93760_Sspa_Oph2007_COI	MC32	1	BB93760_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Absent	Sample	Sspa_Oph2007_COI	MC32	COI	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	GAAGGCCTTCCGATGATC	144.91742
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93760_Upin_Bot2011_COI	MC32	1	BB93760_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Absent	Sample	Upin_Bot2011_COI	MC32	COI	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	CTTTAATTACAGCGTTTTTATTGTTGT	144.91742
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.685	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93761_Mgig_Bot2012_COI	MC29	1	BB93761_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Present	Sample	Mgig_Bot2012_COI	MC29	COI	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	TCTTATTCGTTGGAGACTTTATAACCCT	144.91673
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93762_Cmae_Bot2011_COI	MC26	1	BB93762_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Absent	Sample	Cmae_Bot2011_COI	MC26	COI	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	ATGAACAGTCTATCCTCCTTTAG	144.90518
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93762_Hsan_Wil2021_COI	MC26	1	BB93762_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Absent	Sample	Hsan_Wil2021_COI	MC26	COI	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	CCTGGGCCGGTATAGTAGGT	144.90518
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93762_Msal_Bot2012_COI	MC26	1	BB93762_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Absent	Sample	Msal_Bot2012_COI	MC26	COI	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	GYTAGTTCCRATGATGTTAGCTG	144.90518
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93764_Aamu_Bax2006_COI	MC22	1	BB93764_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Absent	Sample	Aamu_Bax2006_COI	MC22	COI	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	GCACAACCGGGATCTTTACTTC	144.90784
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93764_Pcan_Bot2011_IGS	MC22	1	BB93764_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC22	IGS	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	CGTAATCCTCAGTACTGGCTA	144.90784
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.67	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93764_Sspa_Oph2007_COI	MC22	1	BB93764_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Present	Sample	Sspa_Oph2007_COI	MC22	COI	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	GAAGGCCTTCCGATGATC	144.90784
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93764_Upin_Bot2011_COI	MC22	1	BB93764_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Absent	Sample	Upin_Bot2011_COI	MC22	COI	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	CTTTAATTACAGCGTTTTTATTGTTGT	144.90784
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.86	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93765_Mgig_Bot2012_COI	MC17	1	BB93765_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Present	Sample	Mgig_Bot2012_COI	MC17	COI	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	TCTTATTCGTTGGAGACTTTATAACCCT	144.91022
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93766_Cmae_Bot2011_COI	MC16	1	BB93766_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Absent	Sample	Cmae_Bot2011_COI	MC16	COI	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	ATGAACAGTCTATCCTCCTTTAG	144.91374
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93766_Hsan_Wil2021_COI	MC16	1	BB93766_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Absent	Sample	Hsan_Wil2021_COI	MC16	COI	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	CCTGGGCCGGTATAGTAGGT	144.91374
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93766_Msal_Bot2012_COI	MC16	1	BB93766_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Absent	Sample	Msal_Bot2012_COI	MC16	COI	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	GYTAGTTCCRATGATGTTAGCTG	144.91374
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93768_Aamu_Bax2006_COI	MC12	1	BB93768_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Absent	Sample	Aamu_Bax2006_COI	MC12	COI	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	GCACAACCGGGATCTTTACTTC	144.91052
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93768_Pcan_Bot2011_IGS	MC12	1	BB93768_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC12	IGS	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	CGTAATCCTCAGTACTGGCTA	144.91052
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.465	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93768_Sspa_Oph2007_COI	MC12	1	BB93768_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Present	Sample	Sspa_Oph2007_COI	MC12	COI	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	GAAGGCCTTCCGATGATC	144.91052
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93768_Upin_Bot2011_COI	MC12	1	BB93768_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Absent	Sample	Upin_Bot2011_COI	MC12	COI	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	CTTTAATTACAGCGTTTTTATTGTTGT	144.91052
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.737	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93769_Mgig_Bot2012_COI	MC9	1	BB93769_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Present	Sample	Mgig_Bot2012_COI	MC9	COI	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	TCTTATTCGTTGGAGACTTTATAACCCT	144.92542
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.225	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93771_Aamu_Bax2006_COI	MC96	1	BB93771_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Present	Sample	Aamu_Bax2006_COI	MC96	COI	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	GCACAACCGGGATCTTTACTTC	144.91359
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93771_Pcan_Bot2011_IGS	MC96	1	BB93771_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC96	IGS	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	CGTAATCCTCAGTACTGGCTA	144.91359
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.995	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93771_Sspa_Oph2007_COI	MC96	1	BB93771_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Present	Sample	Sspa_Oph2007_COI	MC96	COI	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	GAAGGCCTTCCGATGATC	144.91359
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93771_Upin_Bot2011_COI	MC96	1	BB93771_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Absent	Sample	Upin_Bot2011_COI	MC96	COI	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	CTTTAATTACAGCGTTTTTATTGTTGT	144.91359
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.469	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93772_Mgig_Bot2012_COI	MC94	1	BB93772_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Present	Sample	Mgig_Bot2012_COI	MC94	COI	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	TCTTATTCGTTGGAGACTTTATAACCCT	144.91439
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93773_Cmae_Bot2011_COI	MC91	1	BB93773_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Absent	Sample	Cmae_Bot2011_COI	MC91	COI	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	ATGAACAGTCTATCCTCCTTTAG	144.91333
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93773_Hsan_Wil2021_COI	MC91	1	BB93773_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Absent	Sample	Hsan_Wil2021_COI	MC91	COI	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	CCTGGGCCGGTATAGTAGGT	144.91333
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93773_Msal_Bot2012_COI	MC91	1	BB93773_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Absent	Sample	Msal_Bot2012_COI	MC91	COI	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	GYTAGTTCCRATGATGTTAGCTG	144.91333
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93775_Aamu_Bax2006_COI	MC88	1	BB93775_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Absent	Sample	Aamu_Bax2006_COI	MC88	COI	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	GCACAACCGGGATCTTTACTTC	144.91409
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93775_Pcan_Bot2011_IGS	MC88	1	BB93775_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC88	IGS	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	CGTAATCCTCAGTACTGGCTA	144.91409
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.242	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93775_Sspa_Oph2007_COI	MC88	1	BB93775_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Present	Sample	Sspa_Oph2007_COI	MC88	COI	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	GAAGGCCTTCCGATGATC	144.91409
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93775_Upin_Bot2011_COI	MC88	1	BB93775_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Absent	Sample	Upin_Bot2011_COI	MC88	COI	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	CTTTAATTACAGCGTTTTTATTGTTGT	144.91409
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.601	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93776_Mgig_Bot2012_COI	MC87	1	BB93776_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Present	Sample	Mgig_Bot2012_COI	MC87	COI	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	TCTTATTCGTTGGAGACTTTATAACCCT	144.92133
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93777_Cmae_Bot2011_COI	MC82	1	BB93777_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Absent	Sample	Cmae_Bot2011_COI	MC82	COI	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	ATGAACAGTCTATCCTCCTTTAG	144.92242
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93777_Hsan_Wil2021_COI	MC82	1	BB93777_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Absent	Sample	Hsan_Wil2021_COI	MC82	COI	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	CCTGGGCCGGTATAGTAGGT	144.92242
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93777_Msal_Bot2012_COI	MC82	1	BB93777_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Absent	Sample	Msal_Bot2012_COI	MC82	COI	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	GYTAGTTCCRATGATGTTAGCTG	144.92242
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93779_Aamu_Bax2006_COI	MC7	1	BB93779_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Absent	Sample	Aamu_Bax2006_COI	MC7	COI	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	GCACAACCGGGATCTTTACTTC	144.92731
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93779_Pcan_Bot2011_IGS	MC7	1	BB93779_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC7	IGS	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	CGTAATCCTCAGTACTGGCTA	144.92731
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.645	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93779_Sspa_Oph2007_COI	MC7	1	BB93779_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Present	Sample	Sspa_Oph2007_COI	MC7	COI	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	GAAGGCCTTCCGATGATC	144.92731
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93779_Upin_Bot2011_COI	MC7	1	BB93779_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Absent	Sample	Upin_Bot2011_COI	MC7	COI	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	CTTTAATTACAGCGTTTTTATTGTTGT	144.92731
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93780_Cmae_Bot2011_COI	MC4	1	BB93780_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Absent	Sample	Cmae_Bot2011_COI	MC4	COI	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	ATGAACAGTCTATCCTCCTTTAG	144.93289
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.6189475	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93780_Hsan_Wil2021_COI	MC4	1	BB93780_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Present	Sample	Hsan_Wil2021_COI	MC4	COI	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	CCTGGGCCGGTATAGTAGGT	144.93289
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93780_Msal_Bot2012_COI	MC4	1	BB93780_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Absent	Sample	Msal_Bot2012_COI	MC4	COI	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	GYTAGTTCCRATGATGTTAGCTG	144.93289
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93782_Aamu_Bax2006_COI	MC1	1	BB93782_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Absent	Sample	Aamu_Bax2006_COI	MC1	COI	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	GCACAACCGGGATCTTTACTTC	144.9291
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93782_Pcan_Bot2011_IGS	MC1	1	BB93782_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC1	IGS	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	CGTAATCCTCAGTACTGGCTA	144.9291
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.129	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93782_Sspa_Oph2007_COI	MC1	1	BB93782_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Present	Sample	Sspa_Oph2007_COI	MC1	COI	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	GAAGGCCTTCCGATGATC	144.9291
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93782_Upin_Bot2011_COI	MC1	1	BB93782_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Absent	Sample	Upin_Bot2011_COI	MC1	COI	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	CTTTAATTACAGCGTTTTTATTGTTGT	144.9291
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.839	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93783_Mgig_Bot2012_COI	MC114	1	BB93783_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Present	Sample	Mgig_Bot2012_COI	MC114	COI	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	TCTTATTCGTTGGAGACTTTATAACCCT	144.90928
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93784_Cmae_Bot2011_COI	MC108	1	BB93784_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Absent	Sample	Cmae_Bot2011_COI	MC108	COI	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	ATGAACAGTCTATCCTCCTTTAG	144.91152
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93784_Hsan_Wil2021_COI	MC108	1	BB93784_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Absent	Sample	Hsan_Wil2021_COI	MC108	COI	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	CCTGGGCCGGTATAGTAGGT	144.91152
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93784_Msal_Bot2012_COI	MC108	1	BB93784_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Absent	Sample	Msal_Bot2012_COI	MC108	COI	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	GYTAGTTCCRATGATGTTAGCTG	144.91152
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93786_Aamu_Bax2006_COI	MC106	1	BB93786_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Absent	Sample	Aamu_Bax2006_COI	MC106	COI	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	GCACAACCGGGATCTTTACTTC	144.91331
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93786_Pcan_Bot2011_IGS	MC106	1	BB93786_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC106	IGS	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	CGTAATCCTCAGTACTGGCTA	144.91331
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.739	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93786_Sspa_Oph2007_COI	MC106	1	BB93786_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Present	Sample	Sspa_Oph2007_COI	MC106	COI	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	GAAGGCCTTCCGATGATC	144.91331
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93786_Upin_Bot2011_COI	MC106	1	BB93786_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Absent	Sample	Upin_Bot2011_COI	MC106	COI	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	CTTTAATTACAGCGTTTTTATTGTTGT	144.91331
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.424	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93787_Mgig_Bot2012_COI	MC104	1	BB93787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Present	Sample	Mgig_Bot2012_COI	MC104	COI	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	TCTTATTCGTTGGAGACTTTATAACCCT	144.91268
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93788_Cmae_Bot2011_COI	MC102	1	BB93788_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Absent	Sample	Cmae_Bot2011_COI	MC102	COI	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	ATGAACAGTCTATCCTCCTTTAG	144.91416
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93788_Hsan_Wil2021_COI	MC102	1	BB93788_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Absent	Sample	Hsan_Wil2021_COI	MC102	COI	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	CCTGGGCCGGTATAGTAGGT	144.91416
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93788_Msal_Bot2012_COI	MC102	1	BB93788_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Absent	Sample	Msal_Bot2012_COI	MC102	COI	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	GYTAGTTCCRATGATGTTAGCTG	144.91416
BB93790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81652	WGS84	BB93790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.474	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93790_Mgig_Bot2012_COI	MC99	1	BB93790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93790	Sample	BB93790	targeted	Present	Sample	Mgig_Bot2012_COI	MC99	COI	45	BB93790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC99	TCTTATTCGTTGGAGACTTTATAACCCT	144.91313
BB93791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85956	WGS84	BB93791	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93791_Cmae_Bot2011_COI	MC126	1	BB93791_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93791	Sample	BB93791	targeted	Absent	Sample	Cmae_Bot2011_COI	MC126	COI	45	BB93791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC126	ATGAACAGTCTATCCTCCTTTAG	144.91138
BB93791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85956	WGS84	BB93791	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93791_Msal_Bot2012_COI	MC126	1	BB93791_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93791	Sample	BB93791	targeted	Absent	Sample	Msal_Bot2012_COI	MC126	COI	45	BB93791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC126	GYTAGTTCCRATGATGTTAGCTG	144.91138
BB93793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8597	WGS84	BB93793	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93793_Aamu_Bax2006_COI	MC123	1	BB93793_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93793	Sample	BB93793	targeted	Absent	Sample	Aamu_Bax2006_COI	MC123	COI	45	BB93793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC123	GCACAACCGGGATCTTTACTTC	144.90663
BB93793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8597	WGS84	BB93793	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93793_Pcan_Bot2011_IGS	MC123	1	BB93793_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93793	Sample	BB93793	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC123	IGS	45	BB93793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC123	CGTAATCCTCAGTACTGGCTA	144.90663
BB93793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8597	WGS84	BB93793	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.124	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93793_Sspa_Oph2007_COI	MC123	1	BB93793_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93793	Sample	BB93793	targeted	Present	Sample	Sspa_Oph2007_COI	MC123	COI	45	BB93793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC123	GAAGGCCTTCCGATGATC	144.90663
BB93793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8597	WGS84	BB93793	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.371	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93793_Upin_Bot2011_COI	MC123	1	BB93793_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93793	Sample	BB93793	targeted	Present	Sample	Upin_Bot2011_COI	MC123	COI	45	BB93793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC123	CTTTAATTACAGCGTTTTTATTGTTGT	144.90663
BB93794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82012	WGS84	BB93794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.714	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93794_Mgig_Bot2012_COI	MC119	1	BB93794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93794	Sample	BB93794	targeted	Present	Sample	Mgig_Bot2012_COI	MC119	COI	45	BB93794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC119	TCTTATTCGTTGGAGACTTTATAACCCT	144.90604
BB93795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93795	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93795_Cmae_Bot2011_COI	MC118	1	BB93795_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93795	Sample	BB93795	targeted	Absent	Sample	Cmae_Bot2011_COI	MC118	COI	45	BB93795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC118	ATGAACAGTCTATCCTCCTTTAG	144.90629
BB93795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93795	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93795_Msal_Bot2012_COI	MC118	1	BB93795_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93795	Sample	BB93795	targeted	Absent	Sample	Msal_Bot2012_COI	MC118	COI	45	BB93795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC118	GYTAGTTCCRATGATGTTAGCTG	144.90629
BB93802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4292	WGS84	BB93802	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93802_Mgig_Bot2012_COI	BC45	1	BB93802_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93802	Sample	BB93802	targeted	Absent	Sample	Mgig_Bot2012_COI	BC45	COI	45	BB93802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC45	TCTTATTCGTTGGAGACTTTATAACCCT	153.12194
BB93803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4271	WGS84	BB93803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93803_Cmae_Bot2011_COI	BC40	1	BB93803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93803	Sample	BB93803	targeted	Absent	Sample	Cmae_Bot2011_COI	BC40	COI	45	BB93803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC40	ATGAACAGTCTATCCTCCTTTAG	153.1238
BB93803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4271	WGS84	BB93803	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93803_Msal_Bot2012_COI	BC40	1	BB93803_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93803	Sample	BB93803	targeted	Absent	Sample	Msal_Bot2012_COI	BC40	COI	45	BB93803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC40	GYTAGTTCCRATGATGTTAGCTG	153.1238
BB93805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44585	WGS84	BB93805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93805_Aamu_Bax2006_COI	BC65	1	BB93805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93805	Sample	BB93805	targeted	Absent	Sample	Aamu_Bax2006_COI	BC65	COI	45	BB93805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC65	GCACAACCGGGATCTTTACTTC	153.08404
BB93805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44585	WGS84	BB93805	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93805_Pcan_Bot2011_IGS	BC65	1	BB93805_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93805	Sample	BB93805	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC65	IGS	45	BB93805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC65	CGTAATCCTCAGTACTGGCTA	153.08404
BB93805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44585	WGS84	BB93805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93805_Sspa_Oph2007_COI	BC65	1	BB93805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93805	Sample	BB93805	targeted	Absent	Sample	Sspa_Oph2007_COI	BC65	COI	45	BB93805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC65	GAAGGCCTTCCGATGATC	153.08404
BB93805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44585	WGS84	BB93805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93805_Upin_Bot2011_COI	BC65	1	BB93805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93805	Sample	BB93805	targeted	Absent	Sample	Upin_Bot2011_COI	BC65	COI	45	BB93805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC65	CTTTAATTACAGCGTTTTTATTGTTGT	153.08404
BB93806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44408	WGS84	BB93806	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93806_Mgig_Bot2012_COI	BC55	1	BB93806_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93806	Sample	BB93806	targeted	Absent	Sample	Mgig_Bot2012_COI	BC55	COI	45	BB93806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC55	TCTTATTCGTTGGAGACTTTATAACCCT	153.07674
BB93807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3627	WGS84	BB93807	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93807_Cmae_Bot2011_COI	BC5	1	BB93807_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93807	Sample	BB93807	targeted	Absent	Sample	Cmae_Bot2011_COI	BC5	COI	45	BB93807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC5	ATGAACAGTCTATCCTCCTTTAG	153.17652
BB93807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3627	WGS84	BB93807	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93807_Msal_Bot2012_COI	BC5	1	BB93807_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93807	Sample	BB93807	targeted	Absent	Sample	Msal_Bot2012_COI	BC5	COI	45	BB93807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC5	GYTAGTTCCRATGATGTTAGCTG	153.17652
BB93809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37857	WGS84	BB93809	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93809_Aamu_Bax2006_COI	BC15	1	BB93809_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93809	Sample	BB93809	targeted	Absent	Sample	Aamu_Bax2006_COI	BC15	COI	45	BB93809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC15	GCACAACCGGGATCTTTACTTC	153.1651
BB93809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37857	WGS84	BB93809	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93809_Pcan_Bot2011_IGS	BC15	1	BB93809_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93809	Sample	BB93809	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC15	IGS	45	BB93809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC15	CGTAATCCTCAGTACTGGCTA	153.1651
BB93809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37857	WGS84	BB93809	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93809_Sspa_Oph2007_COI	BC15	1	BB93809_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93809	Sample	BB93809	targeted	Absent	Sample	Sspa_Oph2007_COI	BC15	COI	45	BB93809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC15	GAAGGCCTTCCGATGATC	153.1651
BB93809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37857	WGS84	BB93809	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93809_Upin_Bot2011_COI	BC15	1	BB93809_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93809	Sample	BB93809	targeted	Absent	Sample	Upin_Bot2011_COI	BC15	COI	45	BB93809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC15	CTTTAATTACAGCGTTTTTATTGTTGT	153.1651
BB93810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44751	WGS84	BB93810	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93810_Cmae_Bot2011_COI	BC50	1	BB93810_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93810	Sample	BB93810	targeted	Absent	Sample	Cmae_Bot2011_COI	BC50	COI	45	BB93810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC50	ATGAACAGTCTATCCTCCTTTAG	153.09566
BB93810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44751	WGS84	BB93810	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93810_Msal_Bot2012_COI	BC50	1	BB93810_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93810	Sample	BB93810	targeted	Absent	Sample	Msal_Bot2012_COI	BC50	COI	45	BB93810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC50	GYTAGTTCCRATGATGTTAGCTG	153.09566
BB93812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43069	WGS84	BB93812	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93812_Aamu_Bax2006_COI	BC47	1	BB93812_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93812	Sample	BB93812	targeted	Absent	Sample	Aamu_Bax2006_COI	BC47	COI	45	BB93812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC47	GCACAACCGGGATCTTTACTTC	153.11845
BB93812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43069	WGS84	BB93812	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93812_Pcan_Bot2011_IGS	BC47	1	BB93812_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93812	Sample	BB93812	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC47	IGS	45	BB93812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC47	CGTAATCCTCAGTACTGGCTA	153.11845
BB93812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43069	WGS84	BB93812	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93812_Sspa_Oph2007_COI	BC47	1	BB93812_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93812	Sample	BB93812	targeted	Absent	Sample	Sspa_Oph2007_COI	BC47	COI	45	BB93812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC47	GAAGGCCTTCCGATGATC	153.11845
BB93812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43069	WGS84	BB93812	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93812_Upin_Bot2011_COI	BC47	1	BB93812_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93812	Sample	BB93812	targeted	Absent	Sample	Upin_Bot2011_COI	BC47	COI	45	BB93812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC47	CTTTAATTACAGCGTTTTTATTGTTGT	153.11845
BB93813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42924	WGS84	BB93813	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93813_Mgig_Bot2012_COI	BC41	1	BB93813_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93813	Sample	BB93813	targeted	Absent	Sample	Mgig_Bot2012_COI	BC41	COI	45	BB93813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC41	TCTTATTCGTTGGAGACTTTATAACCCT	153.1243
BB93814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42466	WGS84	BB93814	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93814_Cmae_Bot2011_COI	BC38	1	BB93814_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93814	Sample	BB93814	targeted	Absent	Sample	Cmae_Bot2011_COI	BC38	COI	45	BB93814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC38	ATGAACAGTCTATCCTCCTTTAG	153.12943
BB93814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42466	WGS84	BB93814	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93814_Msal_Bot2012_COI	BC38	1	BB93814_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93814	Sample	BB93814	targeted	Absent	Sample	Msal_Bot2012_COI	BC38	COI	45	BB93814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC38	GYTAGTTCCRATGATGTTAGCTG	153.12943
BB93816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40652	WGS84	BB93816	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93816_Aamu_Bax2006_COI	BC33	1	BB93816_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93816	Sample	BB93816	targeted	Absent	Sample	Aamu_Bax2006_COI	BC33	COI	45	BB93816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC33	GCACAACCGGGATCTTTACTTC	153.15089
BB93816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40652	WGS84	BB93816	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93816_Pcan_Bot2011_IGS	BC33	1	BB93816_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93816	Sample	BB93816	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC33	IGS	45	BB93816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC33	CGTAATCCTCAGTACTGGCTA	153.15089
BB93816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40652	WGS84	BB93816	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93816_Sspa_Oph2007_COI	BC33	1	BB93816_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93816	Sample	BB93816	targeted	Absent	Sample	Sspa_Oph2007_COI	BC33	COI	45	BB93816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC33	GAAGGCCTTCCGATGATC	153.15089
BB93816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40652	WGS84	BB93816	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93816_Upin_Bot2011_COI	BC33	1	BB93816_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93816	Sample	BB93816	targeted	Absent	Sample	Upin_Bot2011_COI	BC33	COI	45	BB93816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC33	CTTTAATTACAGCGTTTTTATTGTTGT	153.15089
BB93817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4041	WGS84	BB93817	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93817_Mgig_Bot2012_COI	BC31	1	BB93817_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93817	Sample	BB93817	targeted	Absent	Sample	Mgig_Bot2012_COI	BC31	COI	45	BB93817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC31	TCTTATTCGTTGGAGACTTTATAACCCT	153.15312
BB93818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40084	WGS84	BB93818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93818_Cmae_Bot2011_COI	BC29	1	BB93818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93818	Sample	BB93818	targeted	Absent	Sample	Cmae_Bot2011_COI	BC29	COI	45	BB93818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC29	ATGAACAGTCTATCCTCCTTTAG	153.15682
BB93818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40084	WGS84	BB93818	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93818_Msal_Bot2012_COI	BC29	1	BB93818_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93818	Sample	BB93818	targeted	Absent	Sample	Msal_Bot2012_COI	BC29	COI	45	BB93818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC29	GYTAGTTCCRATGATGTTAGCTG	153.15682
BB93820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40096	WGS84	BB93820	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93820_Mgig_Bot2012_COI	BC26	1	BB93820_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93820	Sample	BB93820	targeted	Absent	Sample	Mgig_Bot2012_COI	BC26	COI	45	BB93820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC26	TCTTATTCGTTGGAGACTTTATAACCCT	153.16291
BB93821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44624	WGS84	BB93821	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93821_Cmae_Bot2011_COI	BC62	1	BB93821_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93821	Sample	BB93821	targeted	Absent	Sample	Cmae_Bot2011_COI	BC62	COI	45	BB93821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC62	ATGAACAGTCTATCCTCCTTTAG	153.07816
BB93821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44624	WGS84	BB93821	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93821_Msal_Bot2012_COI	BC62	1	BB93821_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93821	Sample	BB93821	targeted	Absent	Sample	Msal_Bot2012_COI	BC62	COI	45	BB93821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC62	GYTAGTTCCRATGATGTTAGCTG	153.07816
BB93823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44525	WGS84	BB93823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93823_Aamu_Bax2006_COI	BC59	1	BB93823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93823	Sample	BB93823	targeted	Absent	Sample	Aamu_Bax2006_COI	BC59	COI	45	BB93823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC59	GCACAACCGGGATCTTTACTTC	153.07854
BB93823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44525	WGS84	BB93823	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93823_Pcan_Bot2011_IGS	BC59	1	BB93823_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93823	Sample	BB93823	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC59	IGS	45	BB93823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC59	CGTAATCCTCAGTACTGGCTA	153.07854
BB93823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44525	WGS84	BB93823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93823_Sspa_Oph2007_COI	BC59	1	BB93823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93823	Sample	BB93823	targeted	Absent	Sample	Sspa_Oph2007_COI	BC59	COI	45	BB93823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC59	GAAGGCCTTCCGATGATC	153.07854
BB93823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44525	WGS84	BB93823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93823_Upin_Bot2011_COI	BC59	1	BB93823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93823	Sample	BB93823	targeted	Absent	Sample	Upin_Bot2011_COI	BC59	COI	45	BB93823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC59	CTTTAATTACAGCGTTTTTATTGTTGT	153.07854
BB93824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44527	WGS84	BB93824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93824_Mgig_Bot2012_COI	BC56	1	BB93824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93824	Sample	BB93824	targeted	Absent	Sample	Mgig_Bot2012_COI	BC56	COI	45	BB93824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC56	TCTTATTCGTTGGAGACTTTATAACCCT	153.07559
BB93825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44414	WGS84	BB93825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93825_Cmae_Bot2011_COI	BC54	1	BB93825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93825	Sample	BB93825	targeted	Absent	Sample	Cmae_Bot2011_COI	BC54	COI	45	BB93825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC54	ATGAACAGTCTATCCTCCTTTAG	153.07558
BB93825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44414	WGS84	BB93825	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93825_Msal_Bot2012_COI	BC54	1	BB93825_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93825	Sample	BB93825	targeted	Absent	Sample	Msal_Bot2012_COI	BC54	COI	45	BB93825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC54	GYTAGTTCCRATGATGTTAGCTG	153.07558
BB93827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36951	WGS84	BB93827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93827_Aamu_Bax2006_COI	BC9	1	BB93827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93827	Sample	BB93827	targeted	Absent	Sample	Aamu_Bax2006_COI	BC9	COI	45	BB93827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC9	GCACAACCGGGATCTTTACTTC	153.17173
BB93827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36951	WGS84	BB93827	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93827_Pcan_Bot2011_IGS	BC9	1	BB93827_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93827	Sample	BB93827	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC9	IGS	45	BB93827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC9	CGTAATCCTCAGTACTGGCTA	153.17173
BB93827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36951	WGS84	BB93827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93827_Sspa_Oph2007_COI	BC9	1	BB93827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93827	Sample	BB93827	targeted	Absent	Sample	Sspa_Oph2007_COI	BC9	COI	45	BB93827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC9	GAAGGCCTTCCGATGATC	153.17173
BB93827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36951	WGS84	BB93827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93827_Upin_Bot2011_COI	BC9	1	BB93827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93827	Sample	BB93827	targeted	Absent	Sample	Upin_Bot2011_COI	BC9	COI	45	BB93827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC9	CTTTAATTACAGCGTTTTTATTGTTGT	153.17173
BB93828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36653	WGS84	BB93828	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93828_Mgig_Bot2012_COI	BC7	1	BB93828_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93828	Sample	BB93828	targeted	Absent	Sample	Mgig_Bot2012_COI	BC7	COI	45	BB93828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC7	TCTTATTCGTTGGAGACTTTATAACCCT	153.17384
BB93829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36126	WGS84	BB93829	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93829_Cmae_Bot2011_COI	BC4	1	BB93829_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93829	Sample	BB93829	targeted	Absent	Sample	Cmae_Bot2011_COI	BC4	COI	45	BB93829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC4	ATGAACAGTCTATCCTCCTTTAG	153.17768
BB93829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36126	WGS84	BB93829	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93829_Msal_Bot2012_COI	BC4	1	BB93829_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93829	Sample	BB93829	targeted	Absent	Sample	Msal_Bot2012_COI	BC4	COI	45	BB93829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC4	GYTAGTTCCRATGATGTTAGCTG	153.17768
BB93830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35982	WGS84	BB93830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93830_Aamu_Bax2006_COI	BC3	1	BB93830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93830	Sample	BB93830	targeted	Absent	Sample	Aamu_Bax2006_COI	BC3	COI	45	BB93830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC3	GCACAACCGGGATCTTTACTTC	153.17885
BB93830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35982	WGS84	BB93830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93830_Pcan_Bot2011_IGS	BC3	1	BB93830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93830	Sample	BB93830	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC3	IGS	45	BB93830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC3	CGTAATCCTCAGTACTGGCTA	153.17885
BB93830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35982	WGS84	BB93830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93830_Sspa_Oph2007_COI	BC3	1	BB93830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93830	Sample	BB93830	targeted	Absent	Sample	Sspa_Oph2007_COI	BC3	COI	45	BB93830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC3	GAAGGCCTTCCGATGATC	153.17885
BB93830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35982	WGS84	BB93830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93830_Upin_Bot2011_COI	BC3	1	BB93830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93830	Sample	BB93830	targeted	Absent	Sample	Upin_Bot2011_COI	BC3	COI	45	BB93830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC3	CTTTAATTACAGCGTTTTTATTGTTGT	153.17885
BB93831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3909	WGS84	BB93831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93831_Mgig_Bot2012_COI	BC24	1	BB93831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93831	Sample	BB93831	targeted	Absent	Sample	Mgig_Bot2012_COI	BC24	COI	45	BB93831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC24	TCTTATTCGTTGGAGACTTTATAACCCT	153.15723
BB93832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38726	WGS84	BB93832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93832_Cmae_Bot2011_COI	BC22	1	BB93832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93832	Sample	BB93832	targeted	Absent	Sample	Cmae_Bot2011_COI	BC22	COI	45	BB93832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC22	ATGAACAGTCTATCCTCCTTTAG	153.15893
BB93832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38726	WGS84	BB93832	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93832_Msal_Bot2012_COI	BC22	1	BB93832_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93832	Sample	BB93832	targeted	Absent	Sample	Msal_Bot2012_COI	BC22	COI	45	BB93832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC22	GYTAGTTCCRATGATGTTAGCTG	153.15893
BB93834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38037	WGS84	BB93834	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93834_Aamu_Bax2006_COI	BC17	1	BB93834_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93834	Sample	BB93834	targeted	Absent	Sample	Aamu_Bax2006_COI	BC17	COI	45	BB93834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC17	GCACAACCGGGATCTTTACTTC	153.16371
BB93834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38037	WGS84	BB93834	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93834_Pcan_Bot2011_IGS	BC17	1	BB93834_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93834	Sample	BB93834	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC17	IGS	45	BB93834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC17	CGTAATCCTCAGTACTGGCTA	153.16371
BB93834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38037	WGS84	BB93834	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93834_Sspa_Oph2007_COI	BC17	1	BB93834_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93834	Sample	BB93834	targeted	Absent	Sample	Sspa_Oph2007_COI	BC17	COI	45	BB93834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC17	GAAGGCCTTCCGATGATC	153.16371
BB93834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38037	WGS84	BB93834	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93834_Upin_Bot2011_COI	BC17	1	BB93834_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93834	Sample	BB93834	targeted	Absent	Sample	Upin_Bot2011_COI	BC17	COI	45	BB93834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC17	CTTTAATTACAGCGTTTTTATTGTTGT	153.16371
BB93835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37563	WGS84	BB93835	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93835_Mgig_Bot2012_COI	BC13	1	BB93835_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93835	Sample	BB93835	targeted	Absent	Sample	Mgig_Bot2012_COI	BC13	COI	45	BB93835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC13	TCTTATTCGTTGGAGACTTTATAACCCT	153.16734
BB93836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37255	WGS84	BB93836	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93836_Cmae_Bot2011_COI	BC11	1	BB93836_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93836	Sample	BB93836	targeted	Absent	Sample	Cmae_Bot2011_COI	BC11	COI	45	BB93836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC11	ATGAACAGTCTATCCTCCTTTAG	153.16947
BB93836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37255	WGS84	BB93836	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93836_Msal_Bot2012_COI	BC11	1	BB93836_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93836	Sample	BB93836	targeted	Absent	Sample	Msal_Bot2012_COI	BC11	COI	45	BB93836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC11	GYTAGTTCCRATGATGTTAGCTG	153.16947
BB93838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44809	WGS84	BB93838	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93838_Aamu_Bax2006_COI	BC51	1	BB93838_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93838	Sample	BB93838	targeted	Absent	Sample	Aamu_Bax2006_COI	BC51	COI	45	BB93838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC51	GCACAACCGGGATCTTTACTTC	153.09371
BB93838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44809	WGS84	BB93838	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93838_Pcan_Bot2011_IGS	BC51	1	BB93838_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93838	Sample	BB93838	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC51	IGS	45	BB93838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC51	CGTAATCCTCAGTACTGGCTA	153.09371
BB93838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44809	WGS84	BB93838	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93838_Sspa_Oph2007_COI	BC51	1	BB93838_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93838	Sample	BB93838	targeted	Absent	Sample	Sspa_Oph2007_COI	BC51	COI	45	BB93838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC51	GAAGGCCTTCCGATGATC	153.09371
BB93838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44809	WGS84	BB93838	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93838_Upin_Bot2011_COI	BC51	1	BB93838_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93838	Sample	BB93838	targeted	Absent	Sample	Upin_Bot2011_COI	BC51	COI	45	BB93838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC51	CTTTAATTACAGCGTTTTTATTGTTGT	153.09371
BB93854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8765	WGS84	BB93854	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93854_Cmae_Bot2011_COI	HC20	1	BB93854_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93854	Sample	BB93854	targeted	Absent	Sample	Cmae_Bot2011_COI	HC20	COI	45	BB93854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC20	ATGAACAGTCTATCCTCCTTTAG	147.3401
BB93854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8765	WGS84	BB93854	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93854_Msal_Bot2012_COI	HC20	1	BB93854_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93854	Sample	BB93854	targeted	Absent	Sample	Msal_Bot2012_COI	HC20	COI	45	BB93854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC20	GYTAGTTCCRATGATGTTAGCTG	147.3401
BB93856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8745	WGS84	BB93856	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.193	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93856_Aamu_Bax2006_COI	HC23	1	BB93856_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93856	Sample	BB93856	targeted	Present	Sample	Aamu_Bax2006_COI	HC23	COI	45	BB93856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC23	GCACAACCGGGATCTTTACTTC	147.3381
BB93856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8745	WGS84	BB93856	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93856_Pcan_Bot2011_IGS	HC23	1	BB93856_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93856	Sample	BB93856	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC23	IGS	45	BB93856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC23	CGTAATCCTCAGTACTGGCTA	147.3381
BB93856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8745	WGS84	BB93856	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93856_Sspa_Oph2007_COI	HC23	1	BB93856_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93856	Sample	BB93856	targeted	Absent	Sample	Sspa_Oph2007_COI	HC23	COI	45	BB93856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC23	GAAGGCCTTCCGATGATC	147.3381
BB93856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8745	WGS84	BB93856	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93856_Upin_Bot2011_COI	HC23	1	BB93856_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93856	Sample	BB93856	targeted	Absent	Sample	Upin_Bot2011_COI	HC23	COI	45	BB93856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC23	CTTTAATTACAGCGTTTTTATTGTTGT	147.3381
BB93857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.875	WGS84	BB93857	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.221	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93857_Mgig_Bot2012_COI	HC22	1	BB93857_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93857	Sample	BB93857	targeted	Present	Sample	Mgig_Bot2012_COI	HC22	COI	45	BB93857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC22	TCTTATTCGTTGGAGACTTTATAACCCT	147.3394
BB93858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8786	WGS84	BB93858	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93858_Cmae_Bot2011_COI	HC19	1	BB93858_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93858	Sample	BB93858	targeted	Absent	Sample	Cmae_Bot2011_COI	HC19	COI	45	BB93858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC19	ATGAACAGTCTATCCTCCTTTAG	147.3421
BB93858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8786	WGS84	BB93858	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93858_Msal_Bot2012_COI	HC19	1	BB93858_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93858	Sample	BB93858	targeted	Absent	Sample	Msal_Bot2012_COI	HC19	COI	45	BB93858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC19	GYTAGTTCCRATGATGTTAGCTG	147.3421
BB93860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8829	WGS84	BB93860	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.038	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93860_Mgig_Bot2012_COI	HC16	1	BB93860_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93860	Sample	BB93860	targeted	Present	Sample	Mgig_Bot2012_COI	HC16	COI	45	BB93860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC16	TCTTATTCGTTGGAGACTTTATAACCCT	147.3419
BB93861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8859	WGS84	BB93861	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93861_Cmae_Bot2011_COI	HC12	1	BB93861_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93861	Sample	BB93861	targeted	Absent	Sample	Cmae_Bot2011_COI	HC12	COI	45	BB93861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC12	ATGAACAGTCTATCCTCCTTTAG	147.3385
BB93861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8859	WGS84	BB93861	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93861_Msal_Bot2012_COI	HC12	1	BB93861_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93861	Sample	BB93861	targeted	Absent	Sample	Msal_Bot2012_COI	HC12	COI	45	BB93861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC12	GYTAGTTCCRATGATGTTAGCTG	147.3385
BB93863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8485	WGS84	BB93863	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93863_Aamu_Bax2006_COI	HC11	1	BB93863_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93863	Sample	BB93863	targeted	Absent	Sample	Aamu_Bax2006_COI	HC11	COI	45	BB93863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC11	GCACAACCGGGATCTTTACTTC	147.3283
BB93863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8485	WGS84	BB93863	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93863_Pcan_Bot2011_IGS	HC11	1	BB93863_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93863	Sample	BB93863	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC11	IGS	45	BB93863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC11	CGTAATCCTCAGTACTGGCTA	147.3283
BB93863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8485	WGS84	BB93863	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93863_Sspa_Oph2007_COI	HC11	1	BB93863_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93863	Sample	BB93863	targeted	Absent	Sample	Sspa_Oph2007_COI	HC11	COI	45	BB93863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC11	GAAGGCCTTCCGATGATC	147.3283
BB93863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8485	WGS84	BB93863	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93863_Upin_Bot2011_COI	HC11	1	BB93863_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93863	Sample	BB93863	targeted	Absent	Sample	Upin_Bot2011_COI	HC11	COI	45	BB93863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC11	CTTTAATTACAGCGTTTTTATTGTTGT	147.3283
BB93864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8461	WGS84	BB93864	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.375	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93864_Mgig_Bot2012_COI	HC9	1	BB93864_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93864	Sample	BB93864	targeted	Present	Sample	Mgig_Bot2012_COI	HC9	COI	45	BB93864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC9	TCTTATTCGTTGGAGACTTTATAACCCT	147.3297
BB93865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93865	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93865_Cmae_Bot2011_COI	HC8	1	BB93865_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93865	Sample	BB93865	targeted	Absent	Sample	Cmae_Bot2011_COI	HC8	COI	45	BB93865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC8	ATGAACAGTCTATCCTCCTTTAG	147.3242
BB93865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93865	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93865_Msal_Bot2012_COI	HC8	1	BB93865_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93865	Sample	BB93865	targeted	Absent	Sample	Msal_Bot2012_COI	HC8	COI	45	BB93865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC8	GYTAGTTCCRATGATGTTAGCTG	147.3242
BB93867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8284	WGS84	BB93867	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.222	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93867_Aamu_Bax2006_COI	HC3	1	BB93867_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93867	Sample	BB93867	targeted	Present	Sample	Aamu_Bax2006_COI	HC3	COI	45	BB93867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC3	GCACAACCGGGATCTTTACTTC	147.3166
BB93867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8284	WGS84	BB93867	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93867_Pcan_Bot2011_IGS	HC3	1	BB93867_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93867	Sample	BB93867	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC3	IGS	45	BB93867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC3	CGTAATCCTCAGTACTGGCTA	147.3166
BB93867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8284	WGS84	BB93867	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93867_Sspa_Oph2007_COI	HC3	1	BB93867_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93867	Sample	BB93867	targeted	Absent	Sample	Sspa_Oph2007_COI	HC3	COI	45	BB93867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC3	GAAGGCCTTCCGATGATC	147.3166
BB93867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8284	WGS84	BB93867	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93867_Upin_Bot2011_COI	HC3	1	BB93867_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93867	Sample	BB93867	targeted	Absent	Sample	Upin_Bot2011_COI	HC3	COI	45	BB93867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC3	CTTTAATTACAGCGTTTTTATTGTTGT	147.3166
BB93868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.831	WGS84	BB93868	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.2	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93868_Mgig_Bot2012_COI	HC1	1	BB93868_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93868	Sample	BB93868	targeted	Present	Sample	Mgig_Bot2012_COI	HC1	COI	45	BB93868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC1	TCTTATTCGTTGGAGACTTTATAACCCT	147.3215
BB93874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8071	WGS84	BB93874	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93874_Aamu_Bax2006_COI	GC110	1	BB93874_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93874	Sample	BB93874	targeted	Absent	Sample	Aamu_Bax2006_COI	GC110	COI	45	BB93874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC110	GCACAACCGGGATCTTTACTTC	151.21783
BB93874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8071	WGS84	BB93874	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93874_Pcan_Bot2011_IGS	GC110	1	BB93874_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93874	Sample	BB93874	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC110	IGS	45	BB93874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC110	CGTAATCCTCAGTACTGGCTA	151.21783
BB93874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8071	WGS84	BB93874	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93874_Sspa_Oph2007_COI	GC110	1	BB93874_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93874	Sample	BB93874	targeted	Absent	Sample	Sspa_Oph2007_COI	GC110	COI	45	BB93874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC110	GAAGGCCTTCCGATGATC	151.21783
BB93874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8071	WGS84	BB93874	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93874_Upin_Bot2011_COI	GC110	1	BB93874_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93874	Sample	BB93874	targeted	Absent	Sample	Upin_Bot2011_COI	GC110	COI	45	BB93874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC110	CTTTAATTACAGCGTTTTTATTGTTGT	151.21783
BB93875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80585	WGS84	BB93875	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93875_Mgig_Bot2012_COI	GC105	1	BB93875_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93875	Sample	BB93875	targeted	Absent	Sample	Mgig_Bot2012_COI	GC105	COI	45	BB93875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC105	TCTTATTCGTTGGAGACTTTATAACCCT	151.21913
BB93876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85487	WGS84	BB93876	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93876_Cmae_Bot2011_COI	GC10	1	BB93876_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93876	Sample	BB93876	targeted	Absent	Sample	Cmae_Bot2011_COI	GC10	COI	45	BB93876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC10	ATGAACAGTCTATCCTCCTTTAG	151.31994
BB93876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85487	WGS84	BB93876	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93876_Msal_Bot2012_COI	GC10	1	BB93876_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93876	Sample	BB93876	targeted	Absent	Sample	Msal_Bot2012_COI	GC10	COI	45	BB93876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC10	GYTAGTTCCRATGATGTTAGCTG	151.31994
BB93878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79136	WGS84	BB93878	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93878_Aamu_Bax2006_COI	GC100	1	BB93878_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93878	Sample	BB93878	targeted	Absent	Sample	Aamu_Bax2006_COI	GC100	COI	45	BB93878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC100	GCACAACCGGGATCTTTACTTC	151.17803
BB93878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79136	WGS84	BB93878	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93878_Pcan_Bot2011_IGS	GC100	1	BB93878_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93878	Sample	BB93878	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC100	IGS	45	BB93878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC100	CGTAATCCTCAGTACTGGCTA	151.17803
BB93878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79136	WGS84	BB93878	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93878_Sspa_Oph2007_COI	GC100	1	BB93878_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93878	Sample	BB93878	targeted	Absent	Sample	Sspa_Oph2007_COI	GC100	COI	45	BB93878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC100	GAAGGCCTTCCGATGATC	151.17803
BB93878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79136	WGS84	BB93878	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93878_Upin_Bot2011_COI	GC100	1	BB93878_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93878	Sample	BB93878	targeted	Absent	Sample	Upin_Bot2011_COI	GC100	COI	45	BB93878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC100	CTTTAATTACAGCGTTTTTATTGTTGT	151.17803
BB93879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78941	WGS84	BB93879	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93879_Mgig_Bot2012_COI	GC95	1	BB93879_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93879	Sample	BB93879	targeted	Absent	Sample	Mgig_Bot2012_COI	GC95	COI	45	BB93879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC95	TCTTATTCGTTGGAGACTTTATAACCCT	151.18013
BB93881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7864	WGS84	BB93881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93881_Aamu_Bax2006_COI	GC85	1	BB93881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93881	Sample	BB93881	targeted	Absent	Sample	Aamu_Bax2006_COI	GC85	COI	45	BB93881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC85	GCACAACCGGGATCTTTACTTC	151.17264
BB93881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7864	WGS84	BB93881	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93881_Pcan_Bot2011_IGS	GC85	1	BB93881_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93881	Sample	BB93881	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC85	IGS	45	BB93881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC85	CGTAATCCTCAGTACTGGCTA	151.17264
BB93881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7864	WGS84	BB93881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93881_Sspa_Oph2007_COI	GC85	1	BB93881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93881	Sample	BB93881	targeted	Absent	Sample	Sspa_Oph2007_COI	GC85	COI	45	BB93881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC85	GAAGGCCTTCCGATGATC	151.17264
BB93881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7864	WGS84	BB93881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93881_Upin_Bot2011_COI	GC85	1	BB93881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93881	Sample	BB93881	targeted	Absent	Sample	Upin_Bot2011_COI	GC85	COI	45	BB93881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC85	CTTTAATTACAGCGTTTTTATTGTTGT	151.17264
BB93882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78499	WGS84	BB93882	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93882_Mgig_Bot2012_COI	GC80	1	BB93882_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93882	Sample	BB93882	targeted	Absent	Sample	Mgig_Bot2012_COI	GC80	COI	45	BB93882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC80	TCTTATTCGTTGGAGACTTTATAACCCT	151.20342
BB93883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7791	WGS84	BB93883	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93883_Cmae_Bot2011_COI	GC75	1	BB93883_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93883	Sample	BB93883	targeted	Absent	Sample	Cmae_Bot2011_COI	GC75	COI	45	BB93883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC75	ATGAACAGTCTATCCTCCTTTAG	151.19661
BB93883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7791	WGS84	BB93883	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93883_Msal_Bot2012_COI	GC75	1	BB93883_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93883	Sample	BB93883	targeted	Absent	Sample	Msal_Bot2012_COI	GC75	COI	45	BB93883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC75	GYTAGTTCCRATGATGTTAGCTG	151.19661
BB93885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82641	WGS84	BB93885	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93885_Aamu_Bax2006_COI	GC60	1	BB93885_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93885	Sample	BB93885	targeted	Absent	Sample	Aamu_Bax2006_COI	GC60	COI	45	BB93885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC60	GCACAACCGGGATCTTTACTTC	151.24732
BB93885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82641	WGS84	BB93885	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93885_Pcan_Bot2011_IGS	GC60	1	BB93885_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93885	Sample	BB93885	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC60	IGS	45	BB93885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC60	CGTAATCCTCAGTACTGGCTA	151.24732
BB93885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82641	WGS84	BB93885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93885_Sspa_Oph2007_COI	GC60	1	BB93885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93885	Sample	BB93885	targeted	Absent	Sample	Sspa_Oph2007_COI	GC60	COI	45	BB93885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC60	GAAGGCCTTCCGATGATC	151.24732
BB93885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82641	WGS84	BB93885	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93885_Upin_Bot2011_COI	GC60	1	BB93885_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93885	Sample	BB93885	targeted	Absent	Sample	Upin_Bot2011_COI	GC60	COI	45	BB93885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC60	CTTTAATTACAGCGTTTTTATTGTTGT	151.24732
BB93886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8227	WGS84	BB93886	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93886_Mgig_Bot2012_COI	GC55	1	BB93886_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93886	Sample	BB93886	targeted	Absent	Sample	Mgig_Bot2012_COI	GC55	COI	45	BB93886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC55	TCTTATTCGTTGGAGACTTTATAACCCT	151.24297
BB93887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82125	WGS84	BB93887	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93887_Cmae_Bot2011_COI	GC50	1	BB93887_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93887	Sample	BB93887	targeted	Absent	Sample	Cmae_Bot2011_COI	GC50	COI	45	BB93887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC50	ATGAACAGTCTATCCTCCTTTAG	151.23987
BB93887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82125	WGS84	BB93887	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93887_Msal_Bot2012_COI	GC50	1	BB93887_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93887	Sample	BB93887	targeted	Absent	Sample	Msal_Bot2012_COI	GC50	COI	45	BB93887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC50	GYTAGTTCCRATGATGTTAGCTG	151.23987
BB93889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83417	WGS84	BB93889	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93889_Aamu_Bax2006_COI	GC35	1	BB93889_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93889	Sample	BB93889	targeted	Absent	Sample	Aamu_Bax2006_COI	GC35	COI	45	BB93889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC35	GCACAACCGGGATCTTTACTTC	151.2657
BB93889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83417	WGS84	BB93889	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93889_Pcan_Bot2011_IGS	GC35	1	BB93889_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93889	Sample	BB93889	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC35	IGS	45	BB93889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC35	CGTAATCCTCAGTACTGGCTA	151.2657
BB93889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83417	WGS84	BB93889	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93889_Sspa_Oph2007_COI	GC35	1	BB93889_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93889	Sample	BB93889	targeted	Absent	Sample	Sspa_Oph2007_COI	GC35	COI	45	BB93889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC35	GAAGGCCTTCCGATGATC	151.2657
BB93889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83417	WGS84	BB93889	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93889_Upin_Bot2011_COI	GC35	1	BB93889_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93889	Sample	BB93889	targeted	Absent	Sample	Upin_Bot2011_COI	GC35	COI	45	BB93889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC35	CTTTAATTACAGCGTTTTTATTGTTGT	151.2657
BB93890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80771	WGS84	BB93890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93890_Cmae_Bot2011_COI	GC109	1	BB93890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93890	Sample	BB93890	targeted	Absent	Sample	Cmae_Bot2011_COI	GC109	COI	45	BB93890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC109	ATGAACAGTCTATCCTCCTTTAG	151.21666
BB93890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80771	WGS84	BB93890	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93890_Msal_Bot2012_COI	GC109	1	BB93890_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93890	Sample	BB93890	targeted	Absent	Sample	Msal_Bot2012_COI	GC109	COI	45	BB93890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC109	GYTAGTTCCRATGATGTTAGCTG	151.21666
BB93892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80503	WGS84	BB93892	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93892_Aamu_Bax2006_COI	GC103	1	BB93892_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93892	Sample	BB93892	targeted	Absent	Sample	Aamu_Bax2006_COI	GC103	COI	45	BB93892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC103	GCACAACCGGGATCTTTACTTC	151.21647
BB93892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80503	WGS84	BB93892	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93892_Pcan_Bot2011_IGS	GC103	1	BB93892_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93892	Sample	BB93892	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC103	IGS	45	BB93892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC103	CGTAATCCTCAGTACTGGCTA	151.21647
BB93892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80503	WGS84	BB93892	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93892_Sspa_Oph2007_COI	GC103	1	BB93892_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93892	Sample	BB93892	targeted	Absent	Sample	Sspa_Oph2007_COI	GC103	COI	45	BB93892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC103	GAAGGCCTTCCGATGATC	151.21647
BB93892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80503	WGS84	BB93892	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93892_Upin_Bot2011_COI	GC103	1	BB93892_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93892	Sample	BB93892	targeted	Absent	Sample	Upin_Bot2011_COI	GC103	COI	45	BB93892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC103	CTTTAATTACAGCGTTTTTATTGTTGT	151.21647
BB93893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80814	WGS84	BB93893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93893_Mgig_Bot2012_COI	GC101	1	BB93893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93893	Sample	BB93893	targeted	Absent	Sample	Mgig_Bot2012_COI	GC101	COI	45	BB93893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC101	TCTTATTCGTTGGAGACTTTATAACCCT	151.21454
BB93894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85458	WGS84	BB93894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93894_Cmae_Bot2011_COI	GC12	1	BB93894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93894	Sample	BB93894	targeted	Absent	Sample	Cmae_Bot2011_COI	GC12	COI	45	BB93894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC12	ATGAACAGTCTATCCTCCTTTAG	151.32258
BB93894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85458	WGS84	BB93894	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93894_Msal_Bot2012_COI	GC12	1	BB93894_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93894	Sample	BB93894	targeted	Absent	Sample	Msal_Bot2012_COI	GC12	COI	45	BB93894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC12	GYTAGTTCCRATGATGTTAGCTG	151.32258
BB93896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85242	WGS84	BB93896	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93896_Aamu_Bax2006_COI	GC6	1	BB93896_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93896	Sample	BB93896	targeted	Absent	Sample	Aamu_Bax2006_COI	GC6	COI	45	BB93896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC6	GCACAACCGGGATCTTTACTTC	151.31635
BB93896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85242	WGS84	BB93896	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93896_Pcan_Bot2011_IGS	GC6	1	BB93896_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93896	Sample	BB93896	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC6	IGS	45	BB93896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC6	CGTAATCCTCAGTACTGGCTA	151.31635
BB93896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85242	WGS84	BB93896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93896_Sspa_Oph2007_COI	GC6	1	BB93896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93896	Sample	BB93896	targeted	Absent	Sample	Sspa_Oph2007_COI	GC6	COI	45	BB93896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC6	GAAGGCCTTCCGATGATC	151.31635
BB93896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85242	WGS84	BB93896	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93896_Upin_Bot2011_COI	GC6	1	BB93896_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93896	Sample	BB93896	targeted	Absent	Sample	Upin_Bot2011_COI	GC6	COI	45	BB93896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC6	CTTTAATTACAGCGTTTTTATTGTTGT	151.31635
BB93897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85164	WGS84	BB93897	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93897_Mgig_Bot2012_COI	GC3	1	BB93897_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93897	Sample	BB93897	targeted	Absent	Sample	Mgig_Bot2012_COI	GC3	COI	45	BB93897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC3	TCTTATTCGTTGGAGACTTTATAACCCT	151.3116
BB93898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85254	WGS84	BB93898	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93898_Cmae_Bot2011_COI	GC1	1	BB93898_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93898	Sample	BB93898	targeted	Absent	Sample	Cmae_Bot2011_COI	GC1	COI	45	BB93898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC1	ATGAACAGTCTATCCTCCTTTAG	151.30846
BB93898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85254	WGS84	BB93898	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93898_Msal_Bot2012_COI	GC1	1	BB93898_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93898	Sample	BB93898	targeted	Absent	Sample	Msal_Bot2012_COI	GC1	COI	45	BB93898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC1	GYTAGTTCCRATGATGTTAGCTG	151.30846
BB93900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79182	WGS84	BB93900	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93900_Aamu_Bax2006_COI	GC96	1	BB93900_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93900	Sample	BB93900	targeted	Absent	Sample	Aamu_Bax2006_COI	GC96	COI	45	BB93900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC96	GCACAACCGGGATCTTTACTTC	151.18352
BB93900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79182	WGS84	BB93900	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93900_Pcan_Bot2011_IGS	GC96	1	BB93900_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93900	Sample	BB93900	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC96	IGS	45	BB93900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC96	CGTAATCCTCAGTACTGGCTA	151.18352
BB93900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79182	WGS84	BB93900	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93900_Sspa_Oph2007_COI	GC96	1	BB93900_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93900	Sample	BB93900	targeted	Absent	Sample	Sspa_Oph2007_COI	GC96	COI	45	BB93900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC96	GAAGGCCTTCCGATGATC	151.18352
BB93900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79182	WGS84	BB93900	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93900_Upin_Bot2011_COI	GC96	1	BB93900_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93900	Sample	BB93900	targeted	Absent	Sample	Upin_Bot2011_COI	GC96	COI	45	BB93900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC96	CTTTAATTACAGCGTTTTTATTGTTGT	151.18352
BB93901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79014	WGS84	BB93901	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93901_Mgig_Bot2012_COI	GC92	1	BB93901_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93901	Sample	BB93901	targeted	Absent	Sample	Mgig_Bot2012_COI	GC92	COI	45	BB93901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC92	TCTTATTCGTTGGAGACTTTATAACCCT	151.17721
BB93902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78759	WGS84	BB93902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93902_Cmae_Bot2011_COI	GC88	1	BB93902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93902	Sample	BB93902	targeted	Absent	Sample	Cmae_Bot2011_COI	GC88	COI	45	BB93902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC88	ATGAACAGTCTATCCTCCTTTAG	151.17442
BB93902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78759	WGS84	BB93902	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93902_Msal_Bot2012_COI	GC88	1	BB93902_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93902	Sample	BB93902	targeted	Absent	Sample	Msal_Bot2012_COI	GC88	COI	45	BB93902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC88	GYTAGTTCCRATGATGTTAGCTG	151.17442
BB93904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78472	WGS84	BB93904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93904_Aamu_Bax2006_COI	GC84	1	BB93904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93904	Sample	BB93904	targeted	Absent	Sample	Aamu_Bax2006_COI	GC84	COI	45	BB93904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC84	GCACAACCGGGATCTTTACTTC	151.17289
BB93904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78472	WGS84	BB93904	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93904_Pcan_Bot2011_IGS	GC84	1	BB93904_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93904	Sample	BB93904	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC84	IGS	45	BB93904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC84	CGTAATCCTCAGTACTGGCTA	151.17289
BB93904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78472	WGS84	BB93904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93904_Sspa_Oph2007_COI	GC84	1	BB93904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93904	Sample	BB93904	targeted	Absent	Sample	Sspa_Oph2007_COI	GC84	COI	45	BB93904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC84	GAAGGCCTTCCGATGATC	151.17289
BB93904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78472	WGS84	BB93904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93904_Upin_Bot2011_COI	GC84	1	BB93904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93904	Sample	BB93904	targeted	Absent	Sample	Upin_Bot2011_COI	GC84	COI	45	BB93904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC84	CTTTAATTACAGCGTTTTTATTGTTGT	151.17289
BB93905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7855	WGS84	BB93905	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93905_Mgig_Bot2012_COI	GC82	1	BB93905_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93905	Sample	BB93905	targeted	Absent	Sample	Mgig_Bot2012_COI	GC82	COI	45	BB93905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC82	TCTTATTCGTTGGAGACTTTATAACCCT	151.17113
BC05867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83717	WGS84	BC05867	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05867_Aamu_Bax2006_COI	GC14	1	BC05867_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05867	Sample	BC05867	targeted	Absent	Sample	Aamu_Bax2006_COI	GC14	COI	45	BC05867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC14	GCACAACCGGGATCTTTACTTC	151.27458
BC05867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83717	WGS84	BC05867	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05867_Pcan_Bot2011_IGS	GC14	1	BC05867_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05867	Sample	BC05867	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC14	IGS	45	BC05867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC14	CGTAATCCTCAGTACTGGCTA	151.27458
BC05867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83717	WGS84	BC05867	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05867_Sspa_Oph2007_COI	GC14	1	BC05867_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05867	Sample	BC05867	targeted	Absent	Sample	Sspa_Oph2007_COI	GC14	COI	45	BC05867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC14	GAAGGCCTTCCGATGATC	151.27458
BC05867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83717	WGS84	BC05867	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05867_Upin_Bot2011_COI	GC14	1	BC05867_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05867	Sample	BC05867	targeted	Absent	Sample	Upin_Bot2011_COI	GC14	COI	45	BC05867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC14	CTTTAATTACAGCGTTTTTATTGTTGT	151.27458
BC05868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8351	WGS84	BC05868	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05868_Mgig_Bot2012_COI	GC16	1	BC05868_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05868	Sample	BC05868	targeted	Absent	Sample	Mgig_Bot2012_COI	GC16	COI	45	BC05868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC16	TCTTATTCGTTGGAGACTTTATAACCCT	151.27295
BC05869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.835	WGS84	BC05869	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05869_Cmae_Bot2011_COI	GC18	1	BC05869_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05869	Sample	BC05869	targeted	Absent	Sample	Cmae_Bot2011_COI	GC18	COI	45	BC05869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC18	ATGAACAGTCTATCCTCCTTTAG	151.27193
BC05869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.835	WGS84	BC05869	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05869_Msal_Bot2012_COI	GC18	1	BC05869_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05869	Sample	BC05869	targeted	Absent	Sample	Msal_Bot2012_COI	GC18	COI	45	BC05869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC18	GYTAGTTCCRATGATGTTAGCTG	151.27193
BC05870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83558	WGS84	BC05870	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05870_Aamu_Bax2006_COI	GC19	1	BC05870_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05870	Sample	BC05870	targeted	Absent	Sample	Aamu_Bax2006_COI	GC19	COI	45	BC05870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC19	GCACAACCGGGATCTTTACTTC	151.26913
BC05870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83558	WGS84	BC05870	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05870_Pcan_Bot2011_IGS	GC19	1	BC05870_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05870	Sample	BC05870	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC19	IGS	45	BC05870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC19	CGTAATCCTCAGTACTGGCTA	151.26913
BC05870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83558	WGS84	BC05870	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05870_Sspa_Oph2007_COI	GC19	1	BC05870_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05870	Sample	BC05870	targeted	Absent	Sample	Sspa_Oph2007_COI	GC19	COI	45	BC05870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC19	GAAGGCCTTCCGATGATC	151.26913
BC05870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83558	WGS84	BC05870	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05870_Upin_Bot2011_COI	GC19	1	BC05870_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05870	Sample	BC05870	targeted	Absent	Sample	Upin_Bot2011_COI	GC19	COI	45	BC05870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC19	CTTTAATTACAGCGTTTTTATTGTTGT	151.26913
BC05871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83199	WGS84	BC05871	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05871_Mgig_Bot2012_COI	GC21	1	BC05871_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05871	Sample	BC05871	targeted	Absent	Sample	Mgig_Bot2012_COI	GC21	COI	45	BC05871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC21	TCTTATTCGTTGGAGACTTTATAACCCT	151.25933
BC05872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83097	WGS84	BC05872	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05872_Cmae_Bot2011_COI	GC23	1	BC05872_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05872	Sample	BC05872	targeted	Absent	Sample	Cmae_Bot2011_COI	GC23	COI	45	BC05872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC23	ATGAACAGTCTATCCTCCTTTAG	151.2546
BC05872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83097	WGS84	BC05872	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05872_Msal_Bot2012_COI	GC23	1	BC05872_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05872	Sample	BC05872	targeted	Absent	Sample	Msal_Bot2012_COI	GC23	COI	45	BC05872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC23	GYTAGTTCCRATGATGTTAGCTG	151.2546
BC05874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83216	WGS84	BC05874	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05874_Aamu_Bax2006_COI	GC37	1	BC05874_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05874	Sample	BC05874	targeted	Absent	Sample	Aamu_Bax2006_COI	GC37	COI	45	BC05874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC37	GCACAACCGGGATCTTTACTTC	151.26233
BC05874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83216	WGS84	BC05874	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05874_Pcan_Bot2011_IGS	GC37	1	BC05874_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05874	Sample	BC05874	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC37	IGS	45	BC05874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC37	CGTAATCCTCAGTACTGGCTA	151.26233
BC05874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83216	WGS84	BC05874	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05874_Sspa_Oph2007_COI	GC37	1	BC05874_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05874	Sample	BC05874	targeted	Absent	Sample	Sspa_Oph2007_COI	GC37	COI	45	BC05874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC37	GAAGGCCTTCCGATGATC	151.26233
BC05874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83216	WGS84	BC05874	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05874_Upin_Bot2011_COI	GC37	1	BC05874_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05874	Sample	BC05874	targeted	Absent	Sample	Upin_Bot2011_COI	GC37	COI	45	BC05874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC37	CTTTAATTACAGCGTTTTTATTGTTGT	151.26233
BC05875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79288	WGS84	BC05875	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05875_Mgig_Bot2012_COI	GC71	1	BC05875_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05875	Sample	BC05875	targeted	Absent	Sample	Mgig_Bot2012_COI	GC71	COI	45	BC05875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC71	TCTTATTCGTTGGAGACTTTATAACCCT	151.2117
BC05876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79447	WGS84	BC05876	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05876_Cmae_Bot2011_COI	GC72	1	BC05876_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05876	Sample	BC05876	targeted	Absent	Sample	Cmae_Bot2011_COI	GC72	COI	45	BC05876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC72	ATGAACAGTCTATCCTCCTTTAG	151.21339
BC05876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79447	WGS84	BC05876	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05876_Msal_Bot2012_COI	GC72	1	BC05876_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05876	Sample	BC05876	targeted	Absent	Sample	Msal_Bot2012_COI	GC72	COI	45	BC05876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC72	GYTAGTTCCRATGATGTTAGCTG	151.21339
BC05878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81709	WGS84	BC05878	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05878_Aamu_Bax2006_COI	GC24	1	BC05878_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05878	Sample	BC05878	targeted	Absent	Sample	Aamu_Bax2006_COI	GC24	COI	45	BC05878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC24	GCACAACCGGGATCTTTACTTC	151.23804
BC05878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81709	WGS84	BC05878	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05878_Pcan_Bot2011_IGS	GC24	1	BC05878_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05878	Sample	BC05878	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC24	IGS	45	BC05878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC24	CGTAATCCTCAGTACTGGCTA	151.23804
BC05878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81709	WGS84	BC05878	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05878_Sspa_Oph2007_COI	GC24	1	BC05878_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05878	Sample	BC05878	targeted	Absent	Sample	Sspa_Oph2007_COI	GC24	COI	45	BC05878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC24	GAAGGCCTTCCGATGATC	151.23804
BC05878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81709	WGS84	BC05878	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05878_Upin_Bot2011_COI	GC24	1	BC05878_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05878	Sample	BC05878	targeted	Absent	Sample	Upin_Bot2011_COI	GC24	COI	45	BC05878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC24	CTTTAATTACAGCGTTTTTATTGTTGT	151.23804
BC05879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81491	WGS84	BC05879	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05879_Mgig_Bot2012_COI	GC26	1	BC05879_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05879	Sample	BC05879	targeted	Absent	Sample	Mgig_Bot2012_COI	GC26	COI	45	BC05879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC26	TCTTATTCGTTGGAGACTTTATAACCCT	151.23674
BC05881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81904	WGS84	BC05881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05881_Aamu_Bax2006_COI	GC29	1	BC05881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05881	Sample	BC05881	targeted	Absent	Sample	Aamu_Bax2006_COI	GC29	COI	45	BC05881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC29	GCACAACCGGGATCTTTACTTC	151.24007
BC05881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81904	WGS84	BC05881	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05881_Pcan_Bot2011_IGS	GC29	1	BC05881_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05881	Sample	BC05881	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC29	IGS	45	BC05881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC29	CGTAATCCTCAGTACTGGCTA	151.24007
BC05881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81904	WGS84	BC05881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05881_Sspa_Oph2007_COI	GC29	1	BC05881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05881	Sample	BC05881	targeted	Absent	Sample	Sspa_Oph2007_COI	GC29	COI	45	BC05881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC29	GAAGGCCTTCCGATGATC	151.24007
BC05881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81904	WGS84	BC05881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05881_Upin_Bot2011_COI	GC29	1	BC05881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05881	Sample	BC05881	targeted	Absent	Sample	Upin_Bot2011_COI	GC29	COI	45	BC05881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC29	CTTTAATTACAGCGTTTTTATTGTTGT	151.24007
BC05882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82101	WGS84	BC05882	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05882_Mgig_Bot2012_COI	GC31	1	BC05882_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05882	Sample	BC05882	targeted	Absent	Sample	Mgig_Bot2012_COI	GC31	COI	45	BC05882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC31	TCTTATTCGTTGGAGACTTTATAACCCT	151.24208
BC05883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82391	WGS84	BC05883	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05883_Cmae_Bot2011_COI	GC32	1	BC05883_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05883	Sample	BC05883	targeted	Absent	Sample	Cmae_Bot2011_COI	GC32	COI	45	BC05883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC32	ATGAACAGTCTATCCTCCTTTAG	151.24455
BC05883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82391	WGS84	BC05883	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05883_Msal_Bot2012_COI	GC32	1	BC05883_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05883	Sample	BC05883	targeted	Absent	Sample	Msal_Bot2012_COI	GC32	COI	45	BC05883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC32	GYTAGTTCCRATGATGTTAGCTG	151.24455
BC05885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8232	WGS84	BC05885	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05885_Aamu_Bax2006_COI	GC44	1	BC05885_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05885	Sample	BC05885	targeted	Absent	Sample	Aamu_Bax2006_COI	GC44	COI	45	BC05885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC44	GCACAACCGGGATCTTTACTTC	151.24118
BC05885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8232	WGS84	BC05885	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05885_Pcan_Bot2011_IGS	GC44	1	BC05885_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05885	Sample	BC05885	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC44	IGS	45	BC05885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC44	CGTAATCCTCAGTACTGGCTA	151.24118
BC05885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8232	WGS84	BC05885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05885_Sspa_Oph2007_COI	GC44	1	BC05885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05885	Sample	BC05885	targeted	Absent	Sample	Sspa_Oph2007_COI	GC44	COI	45	BC05885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC44	GAAGGCCTTCCGATGATC	151.24118
BC05885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8232	WGS84	BC05885	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05885_Upin_Bot2011_COI	GC44	1	BC05885_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05885	Sample	BC05885	targeted	Absent	Sample	Upin_Bot2011_COI	GC44	COI	45	BC05885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC44	CTTTAATTACAGCGTTTTTATTGTTGT	151.24118
BC05886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8224	WGS84	BC05886	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05886_Mgig_Bot2012_COI	GC49	1	BC05886_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05886	Sample	BC05886	targeted	Absent	Sample	Mgig_Bot2012_COI	GC49	COI	45	BC05886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC49	TCTTATTCGTTGGAGACTTTATAACCCT	151.24152
BC05887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82011	WGS84	BC05887	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05887_Cmae_Bot2011_COI	GC51	1	BC05887_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05887	Sample	BC05887	targeted	Absent	Sample	Cmae_Bot2011_COI	GC51	COI	45	BC05887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC51	ATGAACAGTCTATCCTCCTTTAG	151.23989
BC05887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82011	WGS84	BC05887	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05887_Msal_Bot2012_COI	GC51	1	BC05887_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05887	Sample	BC05887	targeted	Absent	Sample	Msal_Bot2012_COI	GC51	COI	45	BC05887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC51	GYTAGTTCCRATGATGTTAGCTG	151.23989
BC05889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82546	WGS84	BC05889	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05889_Aamu_Bax2006_COI	GC56	1	BC05889_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05889	Sample	BC05889	targeted	Absent	Sample	Aamu_Bax2006_COI	GC56	COI	45	BC05889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC56	GCACAACCGGGATCTTTACTTC	151.24655
BC05889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82546	WGS84	BC05889	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05889_Pcan_Bot2011_IGS	GC56	1	BC05889_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05889	Sample	BC05889	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC56	IGS	45	BC05889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC56	CGTAATCCTCAGTACTGGCTA	151.24655
BC05889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82546	WGS84	BC05889	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05889_Sspa_Oph2007_COI	GC56	1	BC05889_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05889	Sample	BC05889	targeted	Absent	Sample	Sspa_Oph2007_COI	GC56	COI	45	BC05889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC56	GAAGGCCTTCCGATGATC	151.24655
BC05889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82546	WGS84	BC05889	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05889_Upin_Bot2011_COI	GC56	1	BC05889_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05889	Sample	BC05889	targeted	Absent	Sample	Upin_Bot2011_COI	GC56	COI	45	BC05889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC56	CTTTAATTACAGCGTTTTTATTGTTGT	151.24655
BC05890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82703	WGS84	BC05890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05890_Cmae_Bot2011_COI	GC59	1	BC05890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05890	Sample	BC05890	targeted	Absent	Sample	Cmae_Bot2011_COI	GC59	COI	45	BC05890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC59	ATGAACAGTCTATCCTCCTTTAG	151.24639
BC05890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82703	WGS84	BC05890	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05890_Msal_Bot2012_COI	GC59	1	BC05890_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05890	Sample	BC05890	targeted	Absent	Sample	Msal_Bot2012_COI	GC59	COI	45	BC05890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC59	GYTAGTTCCRATGATGTTAGCTG	151.24639
BC05892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82287	WGS84	BC05892	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05892_Aamu_Bax2006_COI	GC62	1	BC05892_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05892	Sample	BC05892	targeted	Absent	Sample	Aamu_Bax2006_COI	GC62	COI	45	BC05892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC62	GCACAACCGGGATCTTTACTTC	151.24617
BC05892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82287	WGS84	BC05892	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05892_Pcan_Bot2011_IGS	GC62	1	BC05892_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05892	Sample	BC05892	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC62	IGS	45	BC05892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC62	CGTAATCCTCAGTACTGGCTA	151.24617
BC05892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82287	WGS84	BC05892	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05892_Sspa_Oph2007_COI	GC62	1	BC05892_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05892	Sample	BC05892	targeted	Absent	Sample	Sspa_Oph2007_COI	GC62	COI	45	BC05892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC62	GAAGGCCTTCCGATGATC	151.24617
BC05892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82287	WGS84	BC05892	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05892_Upin_Bot2011_COI	GC62	1	BC05892_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05892	Sample	BC05892	targeted	Absent	Sample	Upin_Bot2011_COI	GC62	COI	45	BC05892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC62	CTTTAATTACAGCGTTTTTATTGTTGT	151.24617
BC05893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81925	WGS84	BC05893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05893_Mgig_Bot2012_COI	GC63	1	BC05893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05893	Sample	BC05893	targeted	Absent	Sample	Mgig_Bot2012_COI	GC63	COI	45	BC05893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC63	TCTTATTCGTTGGAGACTTTATAACCCT	151.24293
BC05894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81761	WGS84	BC05894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05894_Cmae_Bot2011_COI	GC64	1	BC05894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05894	Sample	BC05894	targeted	Absent	Sample	Cmae_Bot2011_COI	GC64	COI	45	BC05894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC64	ATGAACAGTCTATCCTCCTTTAG	151.24103
BC05894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81761	WGS84	BC05894	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05894_Msal_Bot2012_COI	GC64	1	BC05894_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05894	Sample	BC05894	targeted	Absent	Sample	Msal_Bot2012_COI	GC64	COI	45	BC05894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC64	GYTAGTTCCRATGATGTTAGCTG	151.24103
BC05896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82193	WGS84	BC05896	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05896_Aamu_Bax2006_COI	GC111	1	BC05896_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05896	Sample	BC05896	targeted	Absent	Sample	Aamu_Bax2006_COI	GC111	COI	45	BC05896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC111	GCACAACCGGGATCTTTACTTC	151.24544
BC05896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82193	WGS84	BC05896	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05896_Pcan_Bot2011_IGS	GC111	1	BC05896_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05896	Sample	BC05896	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC111	IGS	45	BC05896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC111	CGTAATCCTCAGTACTGGCTA	151.24544
BC05896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82193	WGS84	BC05896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05896_Sspa_Oph2007_COI	GC111	1	BC05896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05896	Sample	BC05896	targeted	Absent	Sample	Sspa_Oph2007_COI	GC111	COI	45	BC05896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC111	GAAGGCCTTCCGATGATC	151.24544
BC05896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82193	WGS84	BC05896	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05896_Upin_Bot2011_COI	GC111	1	BC05896_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05896	Sample	BC05896	targeted	Absent	Sample	Upin_Bot2011_COI	GC111	COI	45	BC05896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC111	CTTTAATTACAGCGTTTTTATTGTTGT	151.24544
BC05897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78028	WGS84	BC05897	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05897_Mgig_Bot2012_COI	GC77	1	BC05897_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05897	Sample	BC05897	targeted	Absent	Sample	Mgig_Bot2012_COI	GC77	COI	45	BC05897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC77	TCTTATTCGTTGGAGACTTTATAACCCT	151.19927
BC05898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78291	WGS84	BC05898	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05898_Cmae_Bot2011_COI	GC78	1	BC05898_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05898	Sample	BC05898	targeted	Absent	Sample	Cmae_Bot2011_COI	GC78	COI	45	BC05898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC78	ATGAACAGTCTATCCTCCTTTAG	151.19983
BC05898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78291	WGS84	BC05898	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05898_Msal_Bot2012_COI	GC78	1	BC05898_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05898	Sample	BC05898	targeted	Absent	Sample	Msal_Bot2012_COI	GC78	COI	45	BC05898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC78	GYTAGTTCCRATGATGTTAGCTG	151.19983
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.074	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06765_Aamu_Bax2006_COI	T5-01	1	BC06765_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Present	Sample	Aamu_Bax2006_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	GCACAACCGGGATCTTTACTTC	147.81438
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06765_Pcan_Bot2011_IGS	T5-01	1	BC06765_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-01	IGS	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	CGTAATCCTCAGTACTGGCTA	147.81438
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06765_Sspa_Oph2007_COI	T5-01	1	BC06765_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	GAAGGCCTTCCGATGATC	147.81438
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.47	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06765_Upin_Bot2011_COI	T5-01	1	BC06765_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Present	Sample	Upin_Bot2011_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06766_Cjap_Sim2018_COI	T5-02	1	BC06766_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	17/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06766_Mgig_Bot2012_COI	T5-02	1	BC06766_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06766_Mjap_Gib2022_COI	T5-02	1	BC06766_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	AATTTAATTGTGACTAGGCATGGTCTG	147.81438
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06767_Cmae_Bot2011_COI	T5-03	1	BC06767_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	ATGAACAGTCTATCCTCCTTTAG	147.87443
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06767_Mros_Gib2022_COI	T5-03	1	BC06767_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Absent	Sample	Mros_Gib2022_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87443
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06769_Aamu_Bax2006_COI	T5-05	1	BC06769_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Aamu_Bax2006_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	GCACAACCGGGATCTTTACTTC	147.87326
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06769_Pcan_Bot2011_IGS	T5-05	1	BC06769_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-05	IGS	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	CGTAATCCTCAGTACTGGCTA	147.87326
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06769_Sspa_Oph2007_COI	T5-05	1	BC06769_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	GAAGGCCTTCCGATGATC	147.87326
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06769_Upin_Bot2011_COI	T5-05	1	BC06769_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Upin_Bot2011_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.87326
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06770_Cmae_Bot2011_COI	T5-06	1	BC06770_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	ATGAACAGTCTATCCTCCTTTAG	147.87262
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06770_Mros_Gib2022_COI	T5-06	1	BC06770_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Mros_Gib2022_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87262
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.854	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06772_Aamu_Bax2006_COI	T5-08	1	BC06772_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Present	Sample	Aamu_Bax2006_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	GCACAACCGGGATCTTTACTTC	147.3022
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06772_Pcan_Bot2011_IGS	T5-08	1	BC06772_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-08	IGS	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	CGTAATCCTCAGTACTGGCTA	147.3022
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06772_Sspa_Oph2007_COI	T5-08	1	BC06772_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	GAAGGCCTTCCGATGATC	147.3022
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06772_Upin_Bot2011_COI	T5-08	1	BC06772_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Upin_Bot2011_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.3022
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06773_Cjap_Sim2018_COI	T5-09	1	BC06773_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30444
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06773_Mgig_Bot2012_COI	T5-09	1	BC06773_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.30444
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06773_Mjap_Gib2022_COI	T5-09	1	BC06773_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	AATTTAATTGTGACTAGGCATGGTCTG	147.30444
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.765	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06774_Cmae_Bot2011_COI	T5-10	1	BC06774_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Present	Sample	Cmae_Bot2011_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	ATGAACAGTCTATCCTCCTTTAG	147.33697
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	33.086	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06774_Mros_Gib2022_COI	T5-10	1	BC06774_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Present	Sample	Mros_Gib2022_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33697
BC06776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88112	WGS84	BC06776	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.774	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06776_Aamu_Bax2006_COI	T5-12	1	BC06776_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06776	Sample	BC06776	targeted	Present	Sample	Aamu_Bax2006_COI	T5-12	COI	45	BC06776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-12	GCACAACCGGGATCTTTACTTC	147.34198
BC06776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88112	WGS84	BC06776	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06776_Pcan_Bot2011_IGS	T5-12	1	BC06776_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06776	Sample	BC06776	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-12	IGS	45	BC06776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-12	CGTAATCCTCAGTACTGGCTA	147.34198
BC06776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88112	WGS84	BC06776	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06776_Sspa_Oph2007_COI	T5-12	1	BC06776_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06776	Sample	BC06776	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-12	COI	45	BC06776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-12	GAAGGCCTTCCGATGATC	147.34198
BC06776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88112	WGS84	BC06776	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06776_Upin_Bot2011_COI	T5-12	1	BC06776_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06776	Sample	BC06776	targeted	Absent	Sample	Upin_Bot2011_COI	T5-12	COI	45	BC06776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.34198
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06777_Cjap_Sim2018_COI	T5-13	1	BC06777_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33945
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06777_Mgig_Bot2012_COI	T5-13	1	BC06777_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.33945
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06777_Mjap_Gib2022_COI	T5-13	1	BC06777_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	AATTTAATTGTGACTAGGCATGGTCTG	147.33945
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.942	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06778_Cmae_Bot2011_COI	T5-14	1	BC06778_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Present	Sample	Cmae_Bot2011_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	ATGAACAGTCTATCCTCCTTTAG	147.33702
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.011	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06778_Mros_Gib2022_COI	T5-14	1	BC06778_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Present	Sample	Mros_Gib2022_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33702
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06780_Cjap_Sim2018_COI	T5-16	1	BC06780_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53983
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	26/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06780_Mgig_Bot2012_COI	T5-16	1	BC06780_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.53983
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06780_Mjap_Gib2022_COI	T5-16	1	BC06780_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	AATTTAATTGTGACTAGGCATGGTCTG	147.53983
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.218	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06781_Cmae_Bot2011_COI	T5-17	1	BC06781_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Present	Sample	Cmae_Bot2011_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	ATGAACAGTCTATCCTCCTTTAG	147.33607
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.764	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06781_Mros_Gib2022_COI	T5-17	1	BC06781_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Present	Sample	Mros_Gib2022_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33607
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.99	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06783_Aamu_Bax2006_COI	T5-19	1	BC06783_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Present	Sample	Aamu_Bax2006_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	GCACAACCGGGATCTTTACTTC	147.51957
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06783_Pcan_Bot2011_IGS	T5-19	1	BC06783_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-19	IGS	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	CGTAATCCTCAGTACTGGCTA	147.51957
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06783_Sspa_Oph2007_COI	T5-19	1	BC06783_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	GAAGGCCTTCCGATGATC	147.51957
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06783_Upin_Bot2011_COI	T5-19	1	BC06783_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Absent	Sample	Upin_Bot2011_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	CTTTAATTACAGCGTTTTTATTGTTGT	147.51957
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06784_Cjap_Sim2018_COI	T6-01	1	BC06784_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30679
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.463	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06784_Mgig_Bot2012_COI	T6-01	1	BC06784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Present	Sample	Mgig_Bot2012_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.30679
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06784_Mjap_Gib2022_COI	T6-01	1	BC06784_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	AATTTAATTGTGACTAGGCATGGTCTG	147.30679
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06785_Cmae_Bot2011_COI	T6-02	1	BC06785_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	ATGAACAGTCTATCCTCCTTTAG	147.303
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06785_Mros_Gib2022_COI	T6-02	1	BC06785_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Absent	Sample	Mros_Gib2022_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.303
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.184	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06787_Aamu_Bax2006_COI	T6-04	1	BC06787_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Present	Sample	Aamu_Bax2006_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	GCACAACCGGGATCTTTACTTC	147.33639
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06787_Pcan_Bot2011_IGS	T6-04	1	BC06787_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-04	IGS	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	CGTAATCCTCAGTACTGGCTA	147.33639
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06787_Sspa_Oph2007_COI	T6-04	1	BC06787_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	GAAGGCCTTCCGATGATC	147.33639
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06787_Upin_Bot2011_COI	T6-04	1	BC06787_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Absent	Sample	Upin_Bot2011_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.33639
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06788_Cjap_Sim2018_COI	T6-05	1	BC06788_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34157
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.306	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06788_Mgig_Bot2012_COI	T6-05	1	BC06788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Present	Sample	Mgig_Bot2012_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.34157
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06788_Mjap_Gib2022_COI	T6-05	1	BC06788_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	AATTTAATTGTGACTAGGCATGGTCTG	147.34157
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06789_Cmae_Bot2011_COI	T6-06	1	BC06789_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	ATGAACAGTCTATCCTCCTTTAG	147.34153
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06789_Mros_Gib2022_COI	T6-06	1	BC06789_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Absent	Sample	Mros_Gib2022_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34153
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06790_Aamu_Bax2006_COI	T6-07	1	BC06790_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Aamu_Bax2006_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	GCACAACCGGGATCTTTACTTC	147.33483
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06790_Pcan_Bot2011_IGS	T6-07	1	BC06790_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-07	IGS	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	CGTAATCCTCAGTACTGGCTA	147.33483
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06790_Sspa_Oph2007_COI	T6-07	1	BC06790_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	GAAGGCCTTCCGATGATC	147.33483
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06790_Upin_Bot2011_COI	T6-07	1	BC06790_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Upin_Bot2011_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.33483
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06791_Cjap_Sim2018_COI	T6-08	1	BC06791_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33618
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.078	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06791_Mgig_Bot2012_COI	T6-08	1	BC06791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Present	Sample	Mgig_Bot2012_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.33618
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06791_Mjap_Gib2022_COI	T6-08	1	BC06791_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	AATTTAATTGTGACTAGGCATGGTCTG	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06792_Cmae_Bot2011_COI	T6-09	1	BC06792_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	ATGAACAGTCTATCCTCCTTTAG	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06792_Mros_Gib2022_COI	T6-09	1	BC06792_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Absent	Sample	Mros_Gib2022_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33618
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06794_Aamu_Bax2006_COI	T6-11	1	BC06794_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Absent	Sample	Aamu_Bax2006_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	GCACAACCGGGATCTTTACTTC	147.53139
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06794_Pcan_Bot2011_IGS	T6-11	1	BC06794_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-11	IGS	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	CGTAATCCTCAGTACTGGCTA	147.53139
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06794_Sspa_Oph2007_COI	T6-11	1	BC06794_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	GAAGGCCTTCCGATGATC	147.53139
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06794_Upin_Bot2011_COI	T6-11	1	BC06794_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Absent	Sample	Upin_Bot2011_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.53139
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06795_Cjap_Sim2018_COI	T6-12	1	BC06795_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51949
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.164	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	26/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06795_Mgig_Bot2012_COI	T6-12	1	BC06795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Present	Sample	Mgig_Bot2012_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.51949
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06795_Mjap_Gib2022_COI	T6-12	1	BC06795_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	AATTTAATTGTGACTAGGCATGGTCTG	147.51949
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.876	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06796_Cmae_Bot2011_COI	T6-13	1	BC06796_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Present	Sample	Cmae_Bot2011_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	ATGAACAGTCTATCCTCCTTTAG	147.81327
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.812	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06796_Mros_Gib2022_COI	T6-13	1	BC06796_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Present	Sample	Mros_Gib2022_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81327
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06798_Aamu_Bax2006_COI	T6-15	1	BC06798_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Absent	Sample	Aamu_Bax2006_COI	T6-15	COI	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	GCACAACCGGGATCTTTACTTC	147.87386
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06798_Pcan_Bot2011_IGS	T6-15	1	BC06798_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-15	IGS	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	CGTAATCCTCAGTACTGGCTA	147.87386
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06798_Sspa_Oph2007_COI	T6-15	1	BC06798_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-15	COI	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	GAAGGCCTTCCGATGATC	147.87386
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.238	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06798_Upin_Bot2011_COI	T6-15	1	BC06798_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Present	Sample	Upin_Bot2011_COI	T6-15	COI	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.87386
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06799_Cjap_Sim2018_COI	T6-16	1	BC06799_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87236
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	28/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06799_Mgig_Bot2012_COI	T6-16	1	BC06799_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Absent	Sample	Mgig_Bot2012_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.87236
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06799_Mjap_Gib2022_COI	T6-16	1	BC06799_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	AATTTAATTGTGACTAGGCATGGTCTG	147.87236
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.154	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06800_Cmae_Bot2011_COI	T7-01	1	BC06800_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Present	Sample	Cmae_Bot2011_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	ATGAACAGTCTATCCTCCTTTAG	147.81365
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06800_Mros_Gib2022_COI	T7-01	1	BC06800_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Absent	Sample	Mros_Gib2022_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81365
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.402	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06802_Aamu_Bax2006_COI	T7-03	1	BC06802_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Present	Sample	Aamu_Bax2006_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	GCACAACCGGGATCTTTACTTC	147.87392
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06802_Pcan_Bot2011_IGS	T7-03	1	BC06802_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-03	IGS	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	CGTAATCCTCAGTACTGGCTA	147.87392
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06802_Sspa_Oph2007_COI	T7-03	1	BC06802_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	GAAGGCCTTCCGATGATC	147.87392
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.067	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06802_Upin_Bot2011_COI	T7-03	1	BC06802_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Present	Sample	Upin_Bot2011_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.87392
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06803_Cjap_Sim2018_COI	T7-04	1	BC06803_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-04	COI	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87248
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06803_Mgig_Bot2012_COI	T7-04	1	BC06803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Absent	Sample	Mgig_Bot2012_COI	T7-04	COI	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.87248
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.463	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06804_Cmae_Bot2011_COI	T7-05	1	BC06804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Present	Sample	Cmae_Bot2011_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	ATGAACAGTCTATCCTCCTTTAG	147.30842
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.758	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06804_Mros_Gib2022_COI	T7-05	1	BC06804_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Present	Sample	Mros_Gib2022_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30842
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.67	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06806_Aamu_Bax2006_COI	T7-07	1	BC06806_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Present	Sample	Aamu_Bax2006_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	GCACAACCGGGATCTTTACTTC	147.30118
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06806_Pcan_Bot2011_IGS	T7-07	1	BC06806_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-07	IGS	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	CGTAATCCTCAGTACTGGCTA	147.30118
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06806_Sspa_Oph2007_COI	T7-07	1	BC06806_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	GAAGGCCTTCCGATGATC	147.30118
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06806_Upin_Bot2011_COI	T7-07	1	BC06806_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Absent	Sample	Upin_Bot2011_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.30118
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06807_Cjap_Sim2018_COI	T7-08	1	BC06807_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33611
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.68	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06807_Mgig_Bot2012_COI	T7-08	1	BC06807_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Present	Sample	Mgig_Bot2012_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.33611
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06807_Mjap_Gib2022_COI	T7-08	1	BC06807_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	AATTTAATTGTGACTAGGCATGGTCTG	147.33611
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.104	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06808_Cmae_Bot2011_COI	T7-09	1	BC06808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Present	Sample	Cmae_Bot2011_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	ATGAACAGTCTATCCTCCTTTAG	147.34178
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.968	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06808_Mros_Gib2022_COI	T7-09	1	BC06808_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Present	Sample	Mros_Gib2022_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34178
BC06810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88559	WGS84	BC06810	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06810_Cjap_Sim2018_COI	T7-11	1	BC06810_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06810	Sample	BC06810	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-11	COI	45	BC06810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33519
BC06810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88559	WGS84	BC06810	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06810_Mgig_Bot2012_COI	T7-11	1	BC06810_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06810	Sample	BC06810	targeted	Absent	Sample	Mgig_Bot2012_COI	T7-11	COI	45	BC06810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33519
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06811_Cmae_Bot2011_COI	T7-12	1	BC06811_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Absent	Sample	Cmae_Bot2011_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	ATGAACAGTCTATCCTCCTTTAG	147.33607
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	36.436	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06811_Mros_Gib2022_COI	T7-12	1	BC06811_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Present	Sample	Mros_Gib2022_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33607
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.967	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06813_Aamu_Bax2006_COI	T7-14	1	BC06813_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Present	Sample	Aamu_Bax2006_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	GCACAACCGGGATCTTTACTTC	147.53937
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06813_Pcan_Bot2011_IGS	T7-14	1	BC06813_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-14	IGS	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	CGTAATCCTCAGTACTGGCTA	147.53937
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06813_Sspa_Oph2007_COI	T7-14	1	BC06813_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	GAAGGCCTTCCGATGATC	147.53937
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06813_Upin_Bot2011_COI	T7-14	1	BC06813_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Absent	Sample	Upin_Bot2011_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.53937
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06814_Cjap_Sim2018_COI	T7-15	1	BC06814_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53178
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.805	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	28/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06814_Mgig_Bot2012_COI	T7-15	1	BC06814_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Present	Sample	Mgig_Bot2012_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.53178
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06814_Mjap_Gib2022_COI	T7-15	1	BC06814_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	AATTTAATTGTGACTAGGCATGGTCTG	147.53178
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.927	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06815_Cmae_Bot2011_COI	T7-16	1	BC06815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Present	Sample	Cmae_Bot2011_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	ATGAACAGTCTATCCTCCTTTAG	147.51962
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.484	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06815_Mros_Gib2022_COI	T7-16	1	BC06815_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Present	Sample	Mros_Gib2022_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.51962
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06817_Aamu_Bax2006_COI	T8-02	1	BC06817_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Absent	Sample	Aamu_Bax2006_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	GCACAACCGGGATCTTTACTTC	147.87661
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06817_Pcan_Bot2011_IGS	T8-02	1	BC06817_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-02	IGS	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	CGTAATCCTCAGTACTGGCTA	147.87661
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06817_Sspa_Oph2007_COI	T8-02	1	BC06817_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	GAAGGCCTTCCGATGATC	147.87661
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06817_Upin_Bot2011_COI	T8-02	1	BC06817_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Absent	Sample	Upin_Bot2011_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.87661
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06818_Cjap_Sim2018_COI	T8-03	1	BC06818_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87373
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.952	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	17/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06818_Mgig_Bot2012_COI	T8-03	1	BC06818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Present	Sample	Mgig_Bot2012_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.87373
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06818_Mjap_Gib2022_COI	T8-03	1	BC06818_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	AATTTAATTGTGACTAGGCATGGTCTG	147.87373
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06819_Cmae_Bot2011_COI	T8-04	1	BC06819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-04	COI	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	ATGAACAGTCTATCCTCCTTTAG	147.85574
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.736	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06819_Mros_Gib2022_COI	T8-04	1	BC06819_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Present	Sample	Mros_Gib2022_COI	T8-04	COI	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.85574
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06820_Aamu_Bax2006_COI	T8-05	1	BC06820_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Absent	Sample	Aamu_Bax2006_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	GCACAACCGGGATCTTTACTTC	147.81282
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06820_Pcan_Bot2011_IGS	T8-05	1	BC06820_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-05	IGS	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	CGTAATCCTCAGTACTGGCTA	147.81282
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06820_Sspa_Oph2007_COI	T8-05	1	BC06820_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	GAAGGCCTTCCGATGATC	147.81282
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.118	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06820_Upin_Bot2011_COI	T8-05	1	BC06820_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Present	Sample	Upin_Bot2011_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.81282
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06821_Cjap_Sim2018_COI	T8-06	1	BC06821_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3021
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.926	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06821_Mgig_Bot2012_COI	T8-06	1	BC06821_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Present	Sample	Mgig_Bot2012_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.3021
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06821_Mjap_Gib2022_COI	T8-06	1	BC06821_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	AATTTAATTGTGACTAGGCATGGTCTG	147.3021
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06822_Cmae_Bot2011_COI	T8-07	1	BC06822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	ATGAACAGTCTATCCTCCTTTAG	147.30214
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06822_Mros_Gib2022_COI	T8-07	1	BC06822_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Mros_Gib2022_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30214
BC06824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87309	WGS84	BC06824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.436	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06824_Aamu_Bax2006_COI	T8-09	1	BC06824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06824	Sample	BC06824	targeted	Present	Sample	Aamu_Bax2006_COI	T8-09	COI	45	BC06824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-09	GCACAACCGGGATCTTTACTTC	147.33661
BC06824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87309	WGS84	BC06824	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06824_Pcan_Bot2011_IGS	T8-09	1	BC06824_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06824	Sample	BC06824	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-09	IGS	45	BC06824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-09	CGTAATCCTCAGTACTGGCTA	147.33661
BC06824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87309	WGS84	BC06824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06824_Sspa_Oph2007_COI	T8-09	1	BC06824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06824	Sample	BC06824	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-09	COI	45	BC06824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-09	GAAGGCCTTCCGATGATC	147.33661
BC06824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87309	WGS84	BC06824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06824_Upin_Bot2011_COI	T8-09	1	BC06824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06824	Sample	BC06824	targeted	Absent	Sample	Upin_Bot2011_COI	T8-09	COI	45	BC06824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.33661
BC06825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88173	WGS84	BC06825	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06825_Cjap_Sim2018_COI	T8-10	1	BC06825_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06825	Sample	BC06825	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-10	COI	45	BC06825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34203
BC06825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88173	WGS84	BC06825	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	23.602	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06825_Mgig_Bot2012_COI	T8-10	1	BC06825_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06825	Sample	BC06825	targeted	Present	Sample	Mgig_Bot2012_COI	T8-10	COI	45	BC06825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.34203
BC06826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88087	WGS84	BC06826	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06826_Cmae_Bot2011_COI	T8-11	1	BC06826_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06826	Sample	BC06826	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-11	COI	45	BC06826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-11	ATGAACAGTCTATCCTCCTTTAG	147.34167
BC06828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88425	WGS84	BC06828	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.68	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06828_Aamu_Bax2006_COI	T8-13	1	BC06828_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06828	Sample	BC06828	targeted	Present	Sample	Aamu_Bax2006_COI	T8-13	COI	45	BC06828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-13	GCACAACCGGGATCTTTACTTC	147.33592
BC06828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88425	WGS84	BC06828	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06828_Pcan_Bot2011_IGS	T8-13	1	BC06828_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06828	Sample	BC06828	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-13	IGS	45	BC06828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-13	CGTAATCCTCAGTACTGGCTA	147.33592
BC06828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88425	WGS84	BC06828	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06828_Sspa_Oph2007_COI	T8-13	1	BC06828_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06828	Sample	BC06828	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-13	COI	45	BC06828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-13	GAAGGCCTTCCGATGATC	147.33592
BC06828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88425	WGS84	BC06828	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06828_Upin_Bot2011_COI	T8-13	1	BC06828_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06828	Sample	BC06828	targeted	Absent	Sample	Upin_Bot2011_COI	T8-13	COI	45	BC06828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.33592
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06829_Cjap_Sim2018_COI	T8-14	1	BC06829_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33624
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.267	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06829_Mgig_Bot2012_COI	T8-14	1	BC06829_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Present	Sample	Mgig_Bot2012_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.33624
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06829_Mjap_Gib2022_COI	T8-14	1	BC06829_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	AATTTAATTGTGACTAGGCATGGTCTG	147.33624
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.889	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06831_Aamu_Bax2006_COI	T8-16	1	BC06831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Present	Sample	Aamu_Bax2006_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	GCACAACCGGGATCTTTACTTC	147.51943
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06831_Pcan_Bot2011_IGS	T8-16	1	BC06831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-16	IGS	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	CGTAATCCTCAGTACTGGCTA	147.51943
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06831_Sspa_Oph2007_COI	T8-16	1	BC06831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	GAAGGCCTTCCGATGATC	147.51943
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06831_Upin_Bot2011_COI	T8-16	1	BC06831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Absent	Sample	Upin_Bot2011_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.51943
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06832_Cjap_Sim2018_COI	T8-17	1	BC06832_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53022
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.578	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	28/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06832_Mgig_Bot2012_COI	T8-17	1	BC06832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Present	Sample	Mgig_Bot2012_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	TCTTATTCGTTGGAGACTTTATAACCCT	147.53022
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06832_Mjap_Gib2022_COI	T8-17	1	BC06832_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	AATTTAATTGTGACTAGGCATGGTCTG	147.53022
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06833_Cmae_Bot2011_COI	T8-18	1	BC06833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	ATGAACAGTCTATCCTCCTTTAG	147.53883
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.847	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06833_Mros_Gib2022_COI	T8-18	1	BC06833_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Present	Sample	Mros_Gib2022_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53883
BC11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83724	WGS84	BC11603	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.089	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11603_Mgig_Bot2012_COI	S10-02	1	BC11603_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11603	Sample	BC11603	targeted	Present	Sample	Mgig_Bot2012_COI	S10-02	COI	45	BC11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.51054
BC11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78792	WGS84	BC11607	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.725	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11607_Mgig_Bot2012_COI	S10-10	1	BC11607_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11607	Sample	BC11607	targeted	Present	Sample	Mgig_Bot2012_COI	S10-10	COI	45	BC11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.51501
BC11610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84678	WGS84	BC11610	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.754	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11610_Mgig_Bot2012_COI	S10-05	1	BC11610_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11610	Sample	BC11610	targeted	Present	Sample	Mgig_Bot2012_COI	S10-05	COI	45	BC11610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.4973
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11614_Cjap_Sim2018_COI	T15-16	1	BC11614_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53792
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.087	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	30/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11614_Mgig_Bot2012_COI	T15-16	1	BC11614_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Present	Sample	Mgig_Bot2012_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.53792
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11614_Mjap_Gib2022_COI	T15-16	1	BC11614_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	AATTTAATTGTGACTAGGCATGGTCTG	147.53792
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.578	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11615_Cmae_Bot2011_COI	T16-01	1	BC11615_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Present	Sample	Cmae_Bot2011_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	ATGAACAGTCTATCCTCCTTTAG	147.53922
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.179	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11615_Mros_Gib2022_COI	T16-01	1	BC11615_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Present	Sample	Mros_Gib2022_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53922
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.844	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11617_Aamu_Bax2006_COI	T16-03	1	BC11617_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Present	Sample	Aamu_Bax2006_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	GCACAACCGGGATCTTTACTTC	147.52225
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11617_Pcan_Bot2011_IGS	T16-03	1	BC11617_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-03	IGS	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	CGTAATCCTCAGTACTGGCTA	147.52225
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11617_Sspa_Oph2007_COI	T16-03	1	BC11617_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	GAAGGCCTTCCGATGATC	147.52225
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11617_Upin_Bot2011_COI	T16-03	1	BC11617_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Absent	Sample	Upin_Bot2011_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.52225
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11618_Cjap_Sim2018_COI	T16-04	1	BC11618_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51987
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11618_Mgig_Bot2012_COI	T16-04	1	BC11618_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Absent	Sample	Mgig_Bot2012_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.51987
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11618_Mjap_Gib2022_COI	T16-04	1	BC11618_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	AATTTAATTGTGACTAGGCATGGTCTG	147.51987
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11619_Cmae_Bot2011_COI	T16-05	1	BC11619_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Cmae_Bot2011_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	ATGAACAGTCTATCCTCCTTTAG	147.46533
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11619_Mros_Gib2022_COI	T16-05	1	BC11619_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Mros_Gib2022_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.46533
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11620_Aamu_Bax2006_COI	T16-06	1	BC11620_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Aamu_Bax2006_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	GCACAACCGGGATCTTTACTTC	147.48517
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11620_Pcan_Bot2011_IGS	T16-06	1	BC11620_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-06	IGS	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	CGTAATCCTCAGTACTGGCTA	147.48517
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11620_Sspa_Oph2007_COI	T16-06	1	BC11620_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	GAAGGCCTTCCGATGATC	147.48517
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11620_Upin_Bot2011_COI	T16-06	1	BC11620_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Upin_Bot2011_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.48517
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11621_Cjap_Sim2018_COI	T16-07	1	BC11621_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.50225
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11621_Mgig_Bot2012_COI	T16-07	1	BC11621_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Absent	Sample	Mgig_Bot2012_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.50225
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11621_Mjap_Gib2022_COI	T16-07	1	BC11621_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	AATTTAATTGTGACTAGGCATGGTCTG	147.50225
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.557	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11622_Cmae_Bot2011_COI	T16-08	1	BC11622_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Present	Sample	Cmae_Bot2011_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	ATGAACAGTCTATCCTCCTTTAG	147.52327
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11622_Mros_Gib2022_COI	T16-08	1	BC11622_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Absent	Sample	Mros_Gib2022_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.52327
BC11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83707	WGS84	BC11625	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.074	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11625_Mgig_Bot2012_COI	S11-03	1	BC11625_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11625	Sample	BC11625	targeted	Present	Sample	Mgig_Bot2012_COI	S11-03	COI	45	BC11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.50974
BC11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78343	WGS84	BC11629	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.109	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11629_Mgig_Bot2012_COI	S11-07	1	BC11629_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11629	Sample	BC11629	targeted	Present	Sample	Mgig_Bot2012_COI	S11-07	COI	45	BC11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51532
BC11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80912	WGS84	BC11632	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.262	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11632_Mgig_Bot2012_COI	S11-10	1	BC11632_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11632	Sample	BC11632	targeted	Present	Sample	Mgig_Bot2012_COI	S11-10	COI	45	BC11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.51773
BC11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84524	WGS84	BC11636	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.648	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11636_Mgig_Bot2012_COI	S12-02	1	BC11636_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11636	Sample	BC11636	targeted	Present	Sample	Mgig_Bot2012_COI	S12-02	COI	45	BC11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.49705
BC11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78276	WGS84	BC11643	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.436	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11643_Mgig_Bot2012_COI	S12-09	1	BC11643_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11643	Sample	BC11643	targeted	Present	Sample	Mgig_Bot2012_COI	S12-09	COI	45	BC11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51633
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11647_Cjap_Sim2018_COI	T17-01	1	BC11647_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53883
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	11/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11647_Mgig_Bot2012_COI	T17-01	1	BC11647_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Absent	Sample	Mgig_Bot2012_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.53883
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11647_Mjap_Gib2022_COI	T17-01	1	BC11647_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	AATTTAATTGTGACTAGGCATGGTCTG	147.53883
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.594	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11648_Cmae_Bot2011_COI	T17-03	1	BC11648_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Present	Sample	Cmae_Bot2011_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	ATGAACAGTCTATCCTCCTTTAG	147.51982
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.864	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11648_Mros_Gib2022_COI	T17-03	1	BC11648_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Present	Sample	Mros_Gib2022_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.51982
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11650_Cjap_Sim2018_COI	T17-05	1	BC11650_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.47356
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.036	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	22/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11650_Mgig_Bot2012_COI	T17-05	1	BC11650_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Present	Sample	Mgig_Bot2012_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.47356
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11650_Mjap_Gib2022_COI	T17-05	1	BC11650_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	AATTTAATTGTGACTAGGCATGGTCTG	147.47356
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.354	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11651_Cmae_Bot2011_COI	T17-07	1	BC11651_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Present	Sample	Cmae_Bot2011_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	ATGAACAGTCTATCCTCCTTTAG	147.50293
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.008	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11651_Mros_Gib2022_COI	T17-07	1	BC11651_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Present	Sample	Mros_Gib2022_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.50293
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.714	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11653_Aamu_Bax2006_COI	T17-02	1	BC11653_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Present	Sample	Aamu_Bax2006_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	GCACAACCGGGATCTTTACTTC	147.52365
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11653_Pcan_Bot2011_IGS	T17-02	1	BC11653_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-02	IGS	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	CGTAATCCTCAGTACTGGCTA	147.52365
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11653_Sspa_Oph2007_COI	T17-02	1	BC11653_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	GAAGGCCTTCCGATGATC	147.52365
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11653_Upin_Bot2011_COI	T17-02	1	BC11653_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Absent	Sample	Upin_Bot2011_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.52365
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11654_Cjap_Sim2018_COI	T17-06	1	BC11654_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.4865
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	22/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11654_Mgig_Bot2012_COI	T17-06	1	BC11654_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Absent	Sample	Mgig_Bot2012_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.4865
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11654_Mjap_Gib2022_COI	T17-06	1	BC11654_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	AATTTAATTGTGACTAGGCATGGTCTG	147.4865
BC11658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78113	WGS84	BC11658	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.024	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11658_Mgig_Bot2012_COI	S9-02	1	BC11658_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11658	Sample	BC11658	targeted	Present	Sample	Mgig_Bot2012_COI	S9-02	COI	45	BC11658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.51702
BC11661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84516	WGS84	BC11661	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11661_Mgig_Bot2012_COI	S9-05	1	BC11661_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11661	Sample	BC11661	targeted	Absent	Sample	Mgig_Bot2012_COI	S9-05	COI	45	BC11661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.49696
BC11665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83549	WGS84	BC11665	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.648	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11665_Mgig_Bot2012_COI	S9-09	1	BC11665_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11665	Sample	BC11665	targeted	Present	Sample	Mgig_Bot2012_COI	S9-09	COI	45	BC11665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.50816
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.758	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11671_Aamu_Bax2006_COI	T15-15	1	BC11671_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Present	Sample	Aamu_Bax2006_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	GCACAACCGGGATCTTTACTTC	147.5266
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11671_Pcan_Bot2011_IGS	T15-15	1	BC11671_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-15	IGS	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	CGTAATCCTCAGTACTGGCTA	147.5266
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11671_Sspa_Oph2007_COI	T15-15	1	BC11671_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	GAAGGCCTTCCGATGATC	147.5266
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.518	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11671_Upin_Bot2011_COI	T15-15	1	BC11671_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Present	Sample	Upin_Bot2011_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.5266
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11672_Cjap_Sim2018_COI	T15-14	1	BC11672_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51958
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.979	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	30/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11672_Mgig_Bot2012_COI	T15-14	1	BC11672_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Present	Sample	Mgig_Bot2012_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.51958
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11672_Mjap_Gib2022_COI	T15-14	1	BC11672_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	AATTTAATTGTGACTAGGCATGGTCTG	147.51958
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11673_Cmae_Bot2011_COI	T15-13	1	BC11673_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	ATGAACAGTCTATCCTCCTTTAG	147.3371
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11673_Mros_Gib2022_COI	T15-13	1	BC11673_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Absent	Sample	Mros_Gib2022_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.3371
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.345	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11675_Aamu_Bax2006_COI	T15-11	1	BC11675_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Present	Sample	Aamu_Bax2006_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	GCACAACCGGGATCTTTACTTC	147.33592
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11675_Pcan_Bot2011_IGS	T15-11	1	BC11675_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-11	IGS	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	CGTAATCCTCAGTACTGGCTA	147.33592
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11675_Sspa_Oph2007_COI	T15-11	1	BC11675_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	GAAGGCCTTCCGATGATC	147.33592
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11675_Upin_Bot2011_COI	T15-11	1	BC11675_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Absent	Sample	Upin_Bot2011_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33592
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11676_Cjap_Sim2018_COI	T15-10	1	BC11676_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34183
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11676_Mgig_Bot2012_COI	T15-10	1	BC11676_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.34183
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11676_Mjap_Gib2022_COI	T15-10	1	BC11676_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	AATTTAATTGTGACTAGGCATGGTCTG	147.34183
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.832	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11677_Cmae_Bot2011_COI	T15-09	1	BC11677_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Present	Sample	Cmae_Bot2011_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	ATGAACAGTCTATCCTCCTTTAG	147.34172
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11677_Mros_Gib2022_COI	T15-09	1	BC11677_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Absent	Sample	Mros_Gib2022_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34172
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11679_Aamu_Bax2006_COI	T15-07	1	BC11679_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Aamu_Bax2006_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	GCACAACCGGGATCTTTACTTC	147.30774
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11679_Pcan_Bot2011_IGS	T15-07	1	BC11679_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-07	IGS	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	CGTAATCCTCAGTACTGGCTA	147.30774
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11679_Sspa_Oph2007_COI	T15-07	1	BC11679_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	GAAGGCCTTCCGATGATC	147.30774
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11679_Upin_Bot2011_COI	T15-07	1	BC11679_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Upin_Bot2011_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.30774
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11680_Cmae_Bot2011_COI	T15-06	1	BC11680_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	ATGAACAGTCTATCCTCCTTTAG	147.30245
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11680_Mros_Gib2022_COI	T15-06	1	BC11680_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Mros_Gib2022_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30245
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.413	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11682_Aamu_Bax2006_COI	T15-04	1	BC11682_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Present	Sample	Aamu_Bax2006_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	GCACAACCGGGATCTTTACTTC	147.81383
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11682_Pcan_Bot2011_IGS	T15-04	1	BC11682_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-04	IGS	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	CGTAATCCTCAGTACTGGCTA	147.81383
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11682_Sspa_Oph2007_COI	T15-04	1	BC11682_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	GAAGGCCTTCCGATGATC	147.81383
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.992	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11682_Upin_Bot2011_COI	T15-04	1	BC11682_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Present	Sample	Upin_Bot2011_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.81383
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11683_Cjap_Sim2018_COI	T15-03	1	BC11683_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87269
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	24/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11683_Mgig_Bot2012_COI	T15-03	1	BC11683_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.87269
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11683_Mjap_Gib2022_COI	T15-03	1	BC11683_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	AATTTAATTGTGACTAGGCATGGTCTG	147.87269
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11684_Cmae_Bot2011_COI	T15-02	1	BC11684_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	ATGAACAGTCTATCCTCCTTTAG	147.87397
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11684_Mros_Gib2022_COI	T15-02	1	BC11684_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Absent	Sample	Mros_Gib2022_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87397
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.6	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11686_Aamu_Bax2006_COI	T14-11	1	BC11686_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Present	Sample	Aamu_Bax2006_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	GCACAACCGGGATCTTTACTTC	147.34177
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11686_Pcan_Bot2011_IGS	T14-11	1	BC11686_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-11	IGS	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	CGTAATCCTCAGTACTGGCTA	147.34177
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11686_Sspa_Oph2007_COI	T14-11	1	BC11686_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	GAAGGCCTTCCGATGATC	147.34177
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11686_Upin_Bot2011_COI	T14-11	1	BC11686_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Upin_Bot2011_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.34177
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11687_Cjap_Sim2018_COI	T14-15	1	BC11687_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.5197
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	8/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11687_Mgig_Bot2012_COI	T14-15	1	BC11687_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.5197
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11687_Mjap_Gib2022_COI	T14-15	1	BC11687_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	AATTTAATTGTGACTAGGCATGGTCTG	147.5197
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.198	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11688_Cmae_Bot2011_COI	T14-14	1	BC11688_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Present	Sample	Cmae_Bot2011_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	ATGAACAGTCTATCCTCCTTTAG	147.53562
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11688_Mros_Gib2022_COI	T14-14	1	BC11688_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Absent	Sample	Mros_Gib2022_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53562
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11690_Cjap_Sim2018_COI	T14-12	1	BC11690_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34184
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11690_Mgig_Bot2012_COI	T14-12	1	BC11690_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.34184
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11690_Mjap_Gib2022_COI	T14-12	1	BC11690_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	AATTTAATTGTGACTAGGCATGGTCTG	147.34184
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.502	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11691_Cmae_Bot2011_COI	T14-16	1	BC11691_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Present	Sample	Cmae_Bot2011_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	ATGAACAGTCTATCCTCCTTTAG	147.52389
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11691_Mros_Gib2022_COI	T14-16	1	BC11691_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Absent	Sample	Mros_Gib2022_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.52389
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11694_Cjap_Sim2018_COI	T14-08	1	BC11694_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33681
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11694_Mgig_Bot2012_COI	T14-08	1	BC11694_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.33681
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11694_Mjap_Gib2022_COI	T14-08	1	BC11694_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	AATTTAATTGTGACTAGGCATGGTCTG	147.33681
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11695_Cmae_Bot2011_COI	T14-07	1	BC11695_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Cmae_Bot2011_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	ATGAACAGTCTATCCTCCTTTAG	147.30768
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11695_Mros_Gib2022_COI	T14-07	1	BC11695_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Mros_Gib2022_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30768
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11697_Aamu_Bax2006_COI	T14-05	1	BC11697_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Aamu_Bax2006_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	GCACAACCGGGATCTTTACTTC	147.30227
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11697_Pcan_Bot2011_IGS	T14-05	1	BC11697_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-05	IGS	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	CGTAATCCTCAGTACTGGCTA	147.30227
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11697_Sspa_Oph2007_COI	T14-05	1	BC11697_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	GAAGGCCTTCCGATGATC	147.30227
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11697_Upin_Bot2011_COI	T14-05	1	BC11697_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Upin_Bot2011_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.30227
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11698_Cjap_Sim2018_COI	T14-04	1	BC11698_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81605
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	26/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11698_Mgig_Bot2012_COI	T14-04	1	BC11698_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.81605
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11698_Mjap_Gib2022_COI	T14-04	1	BC11698_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	AATTTAATTGTGACTAGGCATGGTCTG	147.81605
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11699_Cmae_Bot2011_COI	T14-03	1	BC11699_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Absent	Sample	Cmae_Bot2011_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	ATGAACAGTCTATCCTCCTTTAG	147.87279
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11699_Mros_Gib2022_COI	T14-03	1	BC11699_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Absent	Sample	Mros_Gib2022_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87279
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.192	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11701_Aamu_Bax2006_COI	T14-01	1	BC11701_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Present	Sample	Aamu_Bax2006_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	GCACAACCGGGATCTTTACTTC	147.87673
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11701_Pcan_Bot2011_IGS	T14-01	1	BC11701_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-01	IGS	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	CGTAATCCTCAGTACTGGCTA	147.87673
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11701_Sspa_Oph2007_COI	T14-01	1	BC11701_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	GAAGGCCTTCCGATGATC	147.87673
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	43.979	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11701_Upin_Bot2011_COI	T14-01	1	BC11701_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Present	Sample	Upin_Bot2011_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.87673
BC15785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88364	WGS84	BC15785	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.669	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15785_Aamu_Bax2006_COI	HA11	0	BC15785_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15785	Sample	BC15785	targeted	Present	Sample	Aamu_Bax2006_COI	HA11	COI	45	BC15785	1.00E-05	Nil	Australia	HA11	GCACAACCGGGATCTTTACTTC	147.33641
BC15785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88364	WGS84	BC15785	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15785_Sspa_Oph2007_COI	HA11	0	BC15785_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15785	Sample	BC15785	targeted	Absent	Sample	Sspa_Oph2007_COI	HA11	COI	45	BC15785	1.00E-05	Nil	Australia	HA11	GAAGGCCTTCCGATGATC	147.33641
BC15785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88364	WGS84	BC15785	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15785_Upin_Bot2011_COI	HA11	0	BC15785_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15785	Sample	BC15785	targeted	Absent	Sample	Upin_Bot2011_COI	HA11	COI	45	BC15785	1.00E-05	Nil	Australia	HA11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33641
BC15786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88289	WGS84	BC15786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15786_Mgig_Bot2012_COI	HA13	0	BC15786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15786	Sample	BC15786	targeted	Absent	Sample	Mgig_Bot2012_COI	HA13	COI	45	BC15786	1.00E-05	Nil	Australia	HA13	TCTTATTCGTTGGAGACTTTATAACCCT	147.33818
BC15787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84498	WGS84	BC15787	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.951	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15787_Cmae_Bot2011_COI	HA19	0	BC15787_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15787	Sample	BC15787	targeted	Present	Sample	Cmae_Bot2011_COI	HA19	COI	45	BC15787	1.00E-05	Nil	Australia	HA19	ATGAACAGTCTATCCTCCTTTAG	147.32942
BC15789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83078	WGS84	BC15789	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15789_Aamu_Bax2006_COI	HA23	0	BC15789_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15789	Sample	BC15789	targeted	Absent	Sample	Aamu_Bax2006_COI	HA23	COI	45	BC15789	1.00E-05	Nil	Australia	HA23	GCACAACCGGGATCTTTACTTC	147.32042
BC15789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83078	WGS84	BC15789	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15789_Sspa_Oph2007_COI	HA23	0	BC15789_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15789	Sample	BC15789	targeted	Absent	Sample	Sspa_Oph2007_COI	HA23	COI	45	BC15789	1.00E-05	Nil	Australia	HA23	GAAGGCCTTCCGATGATC	147.32042
BC15789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83078	WGS84	BC15789	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15789_Upin_Bot2011_COI	HA23	0	BC15789_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15789	Sample	BC15789	targeted	Absent	Sample	Upin_Bot2011_COI	HA23	COI	45	BC15789	1.00E-05	Nil	Australia	HA23	CTTTAATTACAGCGTTTTTATTGTTGT	147.32042
BC15790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88608	WGS84	BC15790	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15790_Cmae_Bot2011_COI	HA3	0	BC15790_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15790	Sample	BC15790	targeted	Absent	Sample	Cmae_Bot2011_COI	HA3	COI	45	BC15790	1.00E-05	Nil	Australia	HA3	ATGAACAGTCTATCCTCCTTTAG	147.33912
BC15792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18675	WGS84	BC15792	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15792_Sspa_Oph2007_COI	DD7	0	BC15792_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15792	Sample	BC15792	targeted	Absent	Sample	Sspa_Oph2007_COI	DD7	COI	45	BC15792	1.00E-05	Nil	Australia	DD7	GAAGGCCTTCCGATGATC	146.36488
BC15792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18675	WGS84	BC15792	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15792_Upin_Bot2011_COI	DD7	0	BC15792_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15792	Sample	BC15792	targeted	Absent	Sample	Upin_Bot2011_COI	DD7	COI	45	BC15792	1.00E-05	Nil	Australia	DD7	CTTTAATTACAGCGTTTTTATTGTTGT	146.36488
BC15793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18467	WGS84	BC15793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15793_Mgig_Bot2012_COI	DD11	0	BC15793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15793	Sample	BC15793	targeted	Absent	Sample	Mgig_Bot2012_COI	DD11	COI	45	BC15793	1.00E-05	Nil	Australia	DD11	TCTTATTCGTTGGAGACTTTATAACCCT	146.36495
BC15794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18373	WGS84	BC15794	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15794_Cmae_Bot2011_COI	DD14	0	BC15794_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15794	Sample	BC15794	targeted	Absent	Sample	Cmae_Bot2011_COI	DD14	COI	45	BC15794	1.00E-05	Nil	Australia	DD14	ATGAACAGTCTATCCTCCTTTAG	146.3646
BC15796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8438	WGS84	BC15796	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.484	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15796_Aamu_Bax2006_COI	MB24	0	BC15796_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15796	Sample	BC15796	targeted	Present	Sample	Aamu_Bax2006_COI	MB24	COI	45	BC15796	1.00E-05	Nil	Australia	MB24	GCACAACCGGGATCTTTACTTC	144.92894
BC15796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8438	WGS84	BC15796	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.62	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15796_Sspa_Oph2007_COI	MB24	0	BC15796_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15796	Sample	BC15796	targeted	Present	Sample	Sspa_Oph2007_COI	MB24	COI	45	BC15796	1.00E-05	Nil	Australia	MB24	GAAGGCCTTCCGATGATC	144.92894
BC15796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8438	WGS84	BC15796	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.546	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15796_Upin_Bot2011_COI	MB24	0	BC15796_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15796	Sample	BC15796	targeted	Present	Sample	Upin_Bot2011_COI	MB24	COI	45	BC15796	1.00E-05	Nil	Australia	MB24	CTTTAATTACAGCGTTTTTATTGTTGT	144.92894
BC15797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84189	WGS84	BC15797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15797_Mgig_Bot2012_COI	MB21	0	BC15797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15797	Sample	BC15797	targeted	Absent	Sample	Mgig_Bot2012_COI	MB21	COI	45	BC15797	1.00E-05	Nil	Australia	MB21	TCTTATTCGTTGGAGACTTTATAACCCT	144.92719
BC15798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84384	WGS84	BC15798	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15798_Cmae_Bot2011_COI	MB19	0	BC15798_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15798	Sample	BC15798	targeted	Absent	Sample	Cmae_Bot2011_COI	MB19	COI	45	BC15798	1.00E-05	Nil	Australia	MB19	ATGAACAGTCTATCCTCCTTTAG	144.92641
BC15800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84782	WGS84	BC15800	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.201	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15800_Aamu_Bax2006_COI	MB14	0	BC15800_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15800	Sample	BC15800	targeted	Present	Sample	Aamu_Bax2006_COI	MB14	COI	45	BC15800	1.00E-05	Nil	Australia	MB14	GCACAACCGGGATCTTTACTTC	144.91026
BC15800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84782	WGS84	BC15800	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.048	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15800_Sspa_Oph2007_COI	MB14	0	BC15800_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15800	Sample	BC15800	targeted	Present	Sample	Sspa_Oph2007_COI	MB14	COI	45	BC15800	1.00E-05	Nil	Australia	MB14	GAAGGCCTTCCGATGATC	144.91026
BC15800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84782	WGS84	BC15800	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.798	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15800_Upin_Bot2011_COI	MB14	0	BC15800_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15800	Sample	BC15800	targeted	Present	Sample	Upin_Bot2011_COI	MB14	COI	45	BC15800	1.00E-05	Nil	Australia	MB14	CTTTAATTACAGCGTTTTTATTGTTGT	144.91026
BC15801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82417	WGS84	BC15801	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.822	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15801_Mgig_Bot2012_COI	MB123	0	BC15801_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15801	Sample	BC15801	targeted	Present	Sample	Mgig_Bot2012_COI	MB123	COI	45	BC15801	1.00E-05	Nil	Australia	MB123	TCTTATTCGTTGGAGACTTTATAACCCT	144.9012
BC15802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82346	WGS84	BC15802	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15802_Cmae_Bot2011_COI	MB122	0	BC15802_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15802	Sample	BC15802	targeted	Absent	Sample	Cmae_Bot2011_COI	MB122	COI	45	BC15802	1.00E-05	Nil	Australia	MB122	ATGAACAGTCTATCCTCCTTTAG	144.90165
BC15804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81895	WGS84	BC15804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.413	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15804_Aamu_Bax2006_COI	MB117	0	BC15804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15804	Sample	BC15804	targeted	Present	Sample	Aamu_Bax2006_COI	MB117	COI	45	BC15804	1.00E-05	Nil	Australia	MB117	GCACAACCGGGATCTTTACTTC	144.90584
BC15804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81895	WGS84	BC15804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.204	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15804_Sspa_Oph2007_COI	MB117	0	BC15804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15804	Sample	BC15804	targeted	Present	Sample	Sspa_Oph2007_COI	MB117	COI	45	BC15804	1.00E-05	Nil	Australia	MB117	GAAGGCCTTCCGATGATC	144.90584
BC15804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81895	WGS84	BC15804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15804_Upin_Bot2011_COI	MB117	0	BC15804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15804	Sample	BC15804	targeted	Absent	Sample	Upin_Bot2011_COI	MB117	COI	45	BC15804	1.00E-05	Nil	Australia	MB117	CTTTAATTACAGCGTTTTTATTGTTGT	144.90584
BC15805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81639	WGS84	BC15805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.491	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15805_Mgig_Bot2012_COI	MB114	0	BC15805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15805	Sample	BC15805	targeted	Present	Sample	Mgig_Bot2012_COI	MB114	COI	45	BC15805	1.00E-05	Nil	Australia	MB114	TCTTATTCGTTGGAGACTTTATAACCCT	144.90628
BC15806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81908	WGS84	BC15806	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15806_Cmae_Bot2011_COI	MB111	0	BC15806_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15806	Sample	BC15806	targeted	Absent	Sample	Cmae_Bot2011_COI	MB111	COI	45	BC15806	1.00E-05	Nil	Australia	MB111	ATGAACAGTCTATCCTCCTTTAG	144.90711
BC15808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81918	WGS84	BC15808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.055	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15808_Aamu_Bax2006_COI	MB107	0	BC15808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15808	Sample	BC15808	targeted	Present	Sample	Aamu_Bax2006_COI	MB107	COI	45	BC15808	1.00E-05	Nil	Australia	MB107	GCACAACCGGGATCTTTACTTC	144.91054
BC15808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81918	WGS84	BC15808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.919	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15808_Sspa_Oph2007_COI	MB107	0	BC15808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15808	Sample	BC15808	targeted	Present	Sample	Sspa_Oph2007_COI	MB107	COI	45	BC15808	1.00E-05	Nil	Australia	MB107	GAAGGCCTTCCGATGATC	144.91054
BC15808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81918	WGS84	BC15808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15808_Upin_Bot2011_COI	MB107	0	BC15808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15808	Sample	BC15808	targeted	Absent	Sample	Upin_Bot2011_COI	MB107	COI	45	BC15808	1.00E-05	Nil	Australia	MB107	CTTTAATTACAGCGTTTTTATTGTTGT	144.91054
BC15809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81839	WGS84	BC15809	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.57	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15809_Mgig_Bot2012_COI	MB106	0	BC15809_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15809	Sample	BC15809	targeted	Present	Sample	Mgig_Bot2012_COI	MB106	COI	45	BC15809	1.00E-05	Nil	Australia	MB106	TCTTATTCGTTGGAGACTTTATAACCCT	144.91417
BC15811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8126	WGS84	BC15811	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15811_Aamu_Bax2006_COI	MB101	0	BC15811_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15811	Sample	BC15811	targeted	Absent	Sample	Aamu_Bax2006_COI	MB101	COI	45	BC15811	1.00E-05	Nil	Australia	MB101	GCACAACCGGGATCTTTACTTC	144.91319
BC15811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8126	WGS84	BC15811	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.063	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15811_Sspa_Oph2007_COI	MB101	0	BC15811_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15811	Sample	BC15811	targeted	Present	Sample	Sspa_Oph2007_COI	MB101	COI	45	BC15811	1.00E-05	Nil	Australia	MB101	GAAGGCCTTCCGATGATC	144.91319
BC15811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8126	WGS84	BC15811	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15811_Upin_Bot2011_COI	MB101	0	BC15811_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15811	Sample	BC15811	targeted	Absent	Sample	Upin_Bot2011_COI	MB101	COI	45	BC15811	1.00E-05	Nil	Australia	MB101	CTTTAATTACAGCGTTTTTATTGTTGT	144.91319
BC15812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.83951	WGS84	BC15812	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15812_Mgig_Bot2012_COI	MB1	0	BC15812_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15812	Sample	BC15812	targeted	Absent	Sample	Mgig_Bot2012_COI	MB1	COI	45	BC15812	1.00E-05	Nil	Australia	MB1	TCTTATTCGTTGGAGACTTTATAACCCT	144.90387
BC15815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81832	WGS84	BC15815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.53	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15815_Aamu_Bax2006_COI	MA94	0	BC15815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15815	Sample	BC15815	targeted	Present	Sample	Aamu_Bax2006_COI	MA94	COI	45	BC15815	1.00E-05	Nil	Australia	MA94	GCACAACCGGGATCTTTACTTC	144.91822
BC15815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81832	WGS84	BC15815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.203	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15815_Sspa_Oph2007_COI	MA94	0	BC15815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15815	Sample	BC15815	targeted	Present	Sample	Sspa_Oph2007_COI	MA94	COI	45	BC15815	1.00E-05	Nil	Australia	MA94	GAAGGCCTTCCGATGATC	144.91822
BC15815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81832	WGS84	BC15815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.642	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15815_Upin_Bot2011_COI	MA94	0	BC15815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15815	Sample	BC15815	targeted	Present	Sample	Upin_Bot2011_COI	MA94	COI	45	BC15815	1.00E-05	Nil	Australia	MA94	CTTTAATTACAGCGTTTTTATTGTTGT	144.91822
BC15816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81807	WGS84	BC15816	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15816_Mgig_Bot2012_COI	MA93	0	BC15816_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15816	Sample	BC15816	targeted	Absent	Sample	Mgig_Bot2012_COI	MA93	COI	45	BC15816	1.00E-05	Nil	Australia	MA93	TCTTATTCGTTGGAGACTTTATAACCCT	144.91519
BC15817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81763	WGS84	BC15817	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15817_Cmae_Bot2011_COI	MA92	0	BC15817_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15817	Sample	BC15817	targeted	Absent	Sample	Cmae_Bot2011_COI	MA92	COI	45	BC15817	1.00E-05	Nil	Australia	MA92	ATGAACAGTCTATCCTCCTTTAG	144.92
BC15819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81923	WGS84	BC15819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.601	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15819_Aamu_Bax2006_COI	MA89	0	BC15819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15819	Sample	BC15819	targeted	Present	Sample	Aamu_Bax2006_COI	MA89	COI	45	BC15819	1.00E-05	Nil	Australia	MA89	GCACAACCGGGATCTTTACTTC	144.91051
BC15819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81923	WGS84	BC15819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15819_Sspa_Oph2007_COI	MA89	0	BC15819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15819	Sample	BC15819	targeted	Absent	Sample	Sspa_Oph2007_COI	MA89	COI	45	BC15819	1.00E-05	Nil	Australia	MA89	GAAGGCCTTCCGATGATC	144.91051
BC15819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81923	WGS84	BC15819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15819_Upin_Bot2011_COI	MA89	0	BC15819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15819	Sample	BC15819	targeted	Absent	Sample	Upin_Bot2011_COI	MA89	COI	45	BC15819	1.00E-05	Nil	Australia	MA89	CTTTAATTACAGCGTTTTTATTGTTGT	144.91051
BC15820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85984	WGS84	BC15820	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.256	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15820_Cmae_Bot2011_COI	MA86	0	BC15820_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15820	Sample	BC15820	targeted	Present	Sample	Cmae_Bot2011_COI	MA86	COI	45	BC15820	1.00E-05	Nil	Australia	MA86	ATGAACAGTCTATCCTCCTTTAG	144.91506
BC15822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86324	WGS84	BC15822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.343	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15822_Aamu_Bax2006_COI	MA83	0	BC15822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15822	Sample	BC15822	targeted	Present	Sample	Aamu_Bax2006_COI	MA83	COI	45	BC15822	1.00E-05	Nil	Australia	MA83	GCACAACCGGGATCTTTACTTC	144.91424
BC15822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86324	WGS84	BC15822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.768	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15822_Sspa_Oph2007_COI	MA83	0	BC15822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15822	Sample	BC15822	targeted	Present	Sample	Sspa_Oph2007_COI	MA83	COI	45	BC15822	1.00E-05	Nil	Australia	MA83	GAAGGCCTTCCGATGATC	144.91424
BC15822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86324	WGS84	BC15822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.828	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15822_Upin_Bot2011_COI	MA83	0	BC15822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15822	Sample	BC15822	targeted	Present	Sample	Upin_Bot2011_COI	MA83	COI	45	BC15822	1.00E-05	Nil	Australia	MA83	CTTTAATTACAGCGTTTTTATTGTTGT	144.91424
BC15823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86202	WGS84	BC15823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15823_Mgig_Bot2012_COI	MA82	0	BC15823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15823	Sample	BC15823	targeted	Absent	Sample	Mgig_Bot2012_COI	MA82	COI	45	BC15823	1.00E-05	Nil	Australia	MA82	TCTTATTCGTTGGAGACTTTATAACCCT	144.91645
BC15824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86328	WGS84	BC15824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15824_Cmae_Bot2011_COI	MA74	0	BC15824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15824	Sample	BC15824	targeted	Absent	Sample	Cmae_Bot2011_COI	MA74	COI	45	BC15824	1.00E-05	Nil	Australia	MA74	ATGAACAGTCTATCCTCCTTTAG	144.91845
BC15827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86208	WGS84	BC15827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15827_Mgig_Bot2012_COI	MA67	0	BC15827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15827	Sample	BC15827	targeted	Absent	Sample	Mgig_Bot2012_COI	MA67	COI	45	BC15827	1.00E-05	Nil	Australia	MA67	TCTTATTCGTTGGAGACTTTATAACCCT	144.91525
BC15830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8263	WGS84	BC15830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15830_Mgig_Bot2012_COI	MA58	0	BC15830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15830	Sample	BC15830	targeted	Absent	Sample	Mgig_Bot2012_COI	MA58	COI	45	BC15830	1.00E-05	Nil	Australia	MA58	TCTTATTCGTTGGAGACTTTATAACCCT	144.90263
BC15833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81978	WGS84	BC15833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.14	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15833_Aamu_Bax2006_COI	MA52	0	BC15833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15833	Sample	BC15833	targeted	Present	Sample	Aamu_Bax2006_COI	MA52	COI	45	BC15833	1.00E-05	Nil	Australia	MA52	GCACAACCGGGATCTTTACTTC	144.92565
BC15833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81978	WGS84	BC15833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.548	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15833_Sspa_Oph2007_COI	MA52	0	BC15833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15833	Sample	BC15833	targeted	Present	Sample	Sspa_Oph2007_COI	MA52	COI	45	BC15833	1.00E-05	Nil	Australia	MA52	GAAGGCCTTCCGATGATC	144.92565
BC15833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81978	WGS84	BC15833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15833_Upin_Bot2011_COI	MA52	0	BC15833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15833	Sample	BC15833	targeted	Absent	Sample	Upin_Bot2011_COI	MA52	COI	45	BC15833	1.00E-05	Nil	Australia	MA52	CTTTAATTACAGCGTTTTTATTGTTGT	144.92565
BC15834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81754	WGS84	BC15834	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15834_Mgig_Bot2012_COI	MA46	0	BC15834_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15834	Sample	BC15834	targeted	Absent	Sample	Mgig_Bot2012_COI	MA46	COI	45	BC15834	1.00E-05	Nil	Australia	MA46	TCTTATTCGTTGGAGACTTTATAACCCT	144.92417
BC15835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85732	WGS84	BC15835	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15835_Cmae_Bot2011_COI	MA44	0	BC15835_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15835	Sample	BC15835	targeted	Absent	Sample	Cmae_Bot2011_COI	MA44	COI	45	BC15835	1.00E-05	Nil	Australia	MA44	ATGAACAGTCTATCCTCCTTTAG	144.91012
BC15837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81973	WGS84	BC15837	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.391	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15837_Aamu_Bax2006_COI	MA4	0	BC15837_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15837	Sample	BC15837	targeted	Present	Sample	Aamu_Bax2006_COI	MA4	COI	45	BC15837	1.00E-05	Nil	Australia	MA4	GCACAACCGGGATCTTTACTTC	144.92989
BC15837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81973	WGS84	BC15837	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.244	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15837_Sspa_Oph2007_COI	MA4	0	BC15837_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15837	Sample	BC15837	targeted	Present	Sample	Sspa_Oph2007_COI	MA4	COI	45	BC15837	1.00E-05	Nil	Australia	MA4	GAAGGCCTTCCGATGATC	144.92989
BC15837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81973	WGS84	BC15837	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15837_Upin_Bot2011_COI	MA4	0	BC15837_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15837	Sample	BC15837	targeted	Absent	Sample	Upin_Bot2011_COI	MA4	COI	45	BC15837	1.00E-05	Nil	Australia	MA4	CTTTAATTACAGCGTTTTTATTGTTGT	144.92989
BC15838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84279	WGS84	BC15838	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15838_Mgig_Bot2012_COI	MA37	0	BC15838_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15838	Sample	BC15838	targeted	Absent	Sample	Mgig_Bot2012_COI	MA37	COI	45	BC15838	1.00E-05	Nil	Australia	MA37	TCTTATTCGTTGGAGACTTTATAACCCT	144.90429
BC15839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84415	WGS84	BC15839	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15839_Cmae_Bot2011_COI	MA36	0	BC15839_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15839	Sample	BC15839	targeted	Absent	Sample	Cmae_Bot2011_COI	MA36	COI	45	BC15839	1.00E-05	Nil	Australia	MA36	ATGAACAGTCTATCCTCCTTTAG	144.90481
BC15840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84748	WGS84	BC15840	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.652	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15840_Aamu_Bax2006_COI	MA34	0	BC15840_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15840	Sample	BC15840	targeted	Present	Sample	Aamu_Bax2006_COI	MA34	COI	45	BC15840	1.00E-05	Nil	Australia	MA34	GCACAACCGGGATCTTTACTTC	144.90998
BC15840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84748	WGS84	BC15840	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.146	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15840_Sspa_Oph2007_COI	MA34	0	BC15840_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15840	Sample	BC15840	targeted	Present	Sample	Sspa_Oph2007_COI	MA34	COI	45	BC15840	1.00E-05	Nil	Australia	MA34	GAAGGCCTTCCGATGATC	144.90998
BC15840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84748	WGS84	BC15840	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15840_Upin_Bot2011_COI	MA34	0	BC15840_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15840	Sample	BC15840	targeted	Absent	Sample	Upin_Bot2011_COI	MA34	COI	45	BC15840	1.00E-05	Nil	Australia	MA34	CTTTAATTACAGCGTTTTTATTGTTGT	144.90998
BC15841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84667	WGS84	BC15841	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15841_Mgig_Bot2012_COI	MA31	0	BC15841_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15841	Sample	BC15841	targeted	Absent	Sample	Mgig_Bot2012_COI	MA31	COI	45	BC15841	1.00E-05	Nil	Australia	MA31	TCTTATTCGTTGGAGACTTTATAACCCT	144.90582
BC15842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81938	WGS84	BC15842	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15842_Cmae_Bot2011_COI	MA3	0	BC15842_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15842	Sample	BC15842	targeted	Absent	Sample	Cmae_Bot2011_COI	MA3	COI	45	BC15842	1.00E-05	Nil	Australia	MA3	ATGAACAGTCTATCCTCCTTTAG	144.92775
BC15844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84362	WGS84	BC15844	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.912	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15844_Aamu_Bax2006_COI	MA28	0	BC15844_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15844	Sample	BC15844	targeted	Present	Sample	Aamu_Bax2006_COI	MA28	COI	45	BC15844	1.00E-05	Nil	Australia	MA28	GCACAACCGGGATCTTTACTTC	144.90725
BC15844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84362	WGS84	BC15844	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.649	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15844_Sspa_Oph2007_COI	MA28	0	BC15844_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15844	Sample	BC15844	targeted	Present	Sample	Sspa_Oph2007_COI	MA28	COI	45	BC15844	1.00E-05	Nil	Australia	MA28	GAAGGCCTTCCGATGATC	144.90725
BC15844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84362	WGS84	BC15844	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.295	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15844_Upin_Bot2011_COI	MA28	0	BC15844_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15844	Sample	BC15844	targeted	Present	Sample	Upin_Bot2011_COI	MA28	COI	45	BC15844	1.00E-05	Nil	Australia	MA28	CTTTAATTACAGCGTTTTTATTGTTGT	144.90725
BC15845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84523	WGS84	BC15845	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15845_Mgig_Bot2012_COI	MA23	0	BC15845_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15845	Sample	BC15845	targeted	Absent	Sample	Mgig_Bot2012_COI	MA23	COI	45	BC15845	1.00E-05	Nil	Australia	MA23	TCTTATTCGTTGGAGACTTTATAACCCT	144.90766
BC15846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84488	WGS84	BC15846	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15846_Cmae_Bot2011_COI	MA124	0	BC15846_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15846	Sample	BC15846	targeted	Absent	Sample	Cmae_Bot2011_COI	MA124	COI	45	BC15846	1.00E-05	Nil	Australia	MA124	ATGAACAGTCTATCCTCCTTTAG	144.93149
BC15848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8601	WGS84	BC15848	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.262	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15848_Aamu_Bax2006_COI	MA122	0	BC15848_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15848	Sample	BC15848	targeted	Present	Sample	Aamu_Bax2006_COI	MA122	COI	45	BC15848	1.00E-05	Nil	Australia	MA122	GCACAACCGGGATCTTTACTTC	144.91211
BC15848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8601	WGS84	BC15848	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.999	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15848_Sspa_Oph2007_COI	MA122	0	BC15848_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15848	Sample	BC15848	targeted	Present	Sample	Sspa_Oph2007_COI	MA122	COI	45	BC15848	1.00E-05	Nil	Australia	MA122	GAAGGCCTTCCGATGATC	144.91211
BC15848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8601	WGS84	BC15848	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.674	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15848_Upin_Bot2011_COI	MA122	0	BC15848_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15848	Sample	BC15848	targeted	Present	Sample	Upin_Bot2011_COI	MA122	COI	45	BC15848	1.00E-05	Nil	Australia	MA122	CTTTAATTACAGCGTTTTTATTGTTGT	144.91211
BC15849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86282	WGS84	BC15849	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15849_Mgig_Bot2012_COI	MA12	0	BC15849_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15849	Sample	BC15849	targeted	Absent	Sample	Mgig_Bot2012_COI	MA12	COI	45	BC15849	1.00E-05	Nil	Australia	MA12	TCTTATTCGTTGGAGACTTTATAACCCT	144.91377
BC15851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82357	WGS84	BC15851	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.7	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15851_Aamu_Bax2006_COI	MA112	0	BC15851_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15851	Sample	BC15851	targeted	Present	Sample	Aamu_Bax2006_COI	MA112	COI	45	BC15851	1.00E-05	Nil	Australia	MA112	GCACAACCGGGATCTTTACTTC	144.90177
BC15851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82357	WGS84	BC15851	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.651	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15851_Sspa_Oph2007_COI	MA112	0	BC15851_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15851	Sample	BC15851	targeted	Present	Sample	Sspa_Oph2007_COI	MA112	COI	45	BC15851	1.00E-05	Nil	Australia	MA112	GAAGGCCTTCCGATGATC	144.90177
BC15851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82357	WGS84	BC15851	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15851_Upin_Bot2011_COI	MA112	0	BC15851_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15851	Sample	BC15851	targeted	Absent	Sample	Upin_Bot2011_COI	MA112	COI	45	BC15851	1.00E-05	Nil	Australia	MA112	CTTTAATTACAGCGTTTTTATTGTTGT	144.90177
BC15852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84545	WGS84	BC15852	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15852_Mgig_Bot2012_COI	MA111	0	BC15852_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15852	Sample	BC15852	targeted	Absent	Sample	Mgig_Bot2012_COI	MA111	COI	45	BC15852	1.00E-05	Nil	Australia	MA111	TCTTATTCGTTGGAGACTTTATAACCCT	144.93045
BC15853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82671	WGS84	BC15853	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15853_Cmae_Bot2011_COI	MA109	0	BC15853_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15853	Sample	BC15853	targeted	Absent	Sample	Cmae_Bot2011_COI	MA109	COI	45	BC15853	1.00E-05	Nil	Australia	MA109	ATGAACAGTCTATCCTCCTTTAG	144.89983
BC15855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82107	WGS84	BC15855	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.35	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15855_Aamu_Bax2006_COI	MA102	0	BC15855_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15855	Sample	BC15855	targeted	Present	Sample	Aamu_Bax2006_COI	MA102	COI	45	BC15855	1.00E-05	Nil	Australia	MA102	GCACAACCGGGATCTTTACTTC	144.9053
BC15855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82107	WGS84	BC15855	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.772	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15855_Sspa_Oph2007_COI	MA102	0	BC15855_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15855	Sample	BC15855	targeted	Present	Sample	Sspa_Oph2007_COI	MA102	COI	45	BC15855	1.00E-05	Nil	Australia	MA102	GAAGGCCTTCCGATGATC	144.9053
BC15855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82107	WGS84	BC15855	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15855_Upin_Bot2011_COI	MA102	0	BC15855_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15855	Sample	BC15855	targeted	Absent	Sample	Upin_Bot2011_COI	MA102	COI	45	BC15855	1.00E-05	Nil	Australia	MA102	CTTTAATTACAGCGTTTTTATTGTTGT	144.9053
BC15856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84654	WGS84	BC15856	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.165	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15856_Mgig_Bot2012_COI	MA101	0	BC15856_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15856	Sample	BC15856	targeted	Present	Sample	Mgig_Bot2012_COI	MA101	COI	45	BC15856	1.00E-05	Nil	Australia	MA101	TCTTATTCGTTGGAGACTTTATAACCCT	144.93095
BC15857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88505	WGS84	BC15857	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15857_Cmae_Bot2011_COI	HD18	0	BC15857_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15857	Sample	BC15857	targeted	Absent	Sample	Cmae_Bot2011_COI	HD18	COI	45	BC15857	1.00E-05	Nil	Australia	HD18	ATGAACAGTCTATCCTCCTTTAG	147.33662
BC15859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83088	WGS84	BC15859	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15859_Aamu_Bax2006_COI	HD1	0	BC15859_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15859	Sample	BC15859	targeted	Absent	Sample	Aamu_Bax2006_COI	HD1	COI	45	BC15859	1.00E-05	Nil	Australia	HD1	GCACAACCGGGATCTTTACTTC	147.32074
BC15859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83088	WGS84	BC15859	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15859_Sspa_Oph2007_COI	HD1	0	BC15859_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15859	Sample	BC15859	targeted	Absent	Sample	Sspa_Oph2007_COI	HD1	COI	45	BC15859	1.00E-05	Nil	Australia	HD1	GAAGGCCTTCCGATGATC	147.32074
BC15859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83088	WGS84	BC15859	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15859_Upin_Bot2011_COI	HD1	0	BC15859_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15859	Sample	BC15859	targeted	Absent	Sample	Upin_Bot2011_COI	HD1	COI	45	BC15859	1.00E-05	Nil	Australia	HD1	CTTTAATTACAGCGTTTTTATTGTTGT	147.32074
BC15860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8446	WGS84	BC15860	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15860_Cmae_Bot2011_COI	HC7	0	BC15860_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15860	Sample	BC15860	targeted	Absent	Sample	Cmae_Bot2011_COI	HC7	COI	45	BC15860	1.00E-05	Nil	Australia	HC7	ATGAACAGTCTATCCTCCTTTAG	147.3313
BC15862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.873	WGS84	BC15862	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15862_Aamu_Bax2006_COI	HC24	0	BC15862_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15862	Sample	BC15862	targeted	Absent	Sample	Aamu_Bax2006_COI	HC24	COI	45	BC15862	1.00E-05	Nil	Australia	HC24	GCACAACCGGGATCTTTACTTC	147.3366
BC15862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.873	WGS84	BC15862	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15862_Sspa_Oph2007_COI	HC24	0	BC15862_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15862	Sample	BC15862	targeted	Absent	Sample	Sspa_Oph2007_COI	HC24	COI	45	BC15862	1.00E-05	Nil	Australia	HC24	GAAGGCCTTCCGATGATC	147.3366
BC15862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.873	WGS84	BC15862	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15862_Upin_Bot2011_COI	HC24	0	BC15862_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15862	Sample	BC15862	targeted	Absent	Sample	Upin_Bot2011_COI	HC24	COI	45	BC15862	1.00E-05	Nil	Australia	HC24	CTTTAATTACAGCGTTTTTATTGTTGT	147.3366
BC15863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8759	WGS84	BC15863	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.053	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15863_Mgig_Bot2012_COI	HC21	0	BC15863_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15863	Sample	BC15863	targeted	Present	Sample	Mgig_Bot2012_COI	HC21	COI	45	BC15863	1.00E-05	Nil	Australia	HC21	TCTTATTCGTTGGAGACTTTATAACCCT	147.3413
BC15864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8293	WGS84	BC15864	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15864_Cmae_Bot2011_COI	HC2	0	BC15864_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15864	Sample	BC15864	targeted	Absent	Sample	Cmae_Bot2011_COI	HC2	COI	45	BC15864	1.00E-05	Nil	Australia	HC2	ATGAACAGTCTATCCTCCTTTAG	147.3182
BC15866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8839	WGS84	BC15866	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.233	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15866_Aamu_Bax2006_COI	HC14	0	BC15866_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15866	Sample	BC15866	targeted	Present	Sample	Aamu_Bax2006_COI	HC14	COI	45	BC15866	1.00E-05	Nil	Australia	HC14	GCACAACCGGGATCTTTACTTC	147.3365
BC15866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8839	WGS84	BC15866	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15866_Sspa_Oph2007_COI	HC14	0	BC15866_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15866	Sample	BC15866	targeted	Absent	Sample	Sspa_Oph2007_COI	HC14	COI	45	BC15866	1.00E-05	Nil	Australia	HC14	GAAGGCCTTCCGATGATC	147.3365
BC15866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8839	WGS84	BC15866	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.438	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15866_Upin_Bot2011_COI	HC14	0	BC15866_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15866	Sample	BC15866	targeted	Present	Sample	Upin_Bot2011_COI	HC14	COI	45	BC15866	1.00E-05	Nil	Australia	HC14	CTTTAATTACAGCGTTTTTATTGTTGT	147.3365
BC15867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88525	WGS84	BC15867	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15867_Mgig_Bot2012_COI	HB4	0	BC15867_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15867	Sample	BC15867	targeted	Absent	Sample	Mgig_Bot2012_COI	HB4	COI	45	BC15867	1.00E-05	Nil	Australia	HB4	TCTTATTCGTTGGAGACTTTATAACCCT	147.33569
BC15868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88502	WGS84	BC15868	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15868_Cmae_Bot2011_COI	HB3	0	BC15868_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15868	Sample	BC15868	targeted	Absent	Sample	Cmae_Bot2011_COI	HB3	COI	45	BC15868	1.00E-05	Nil	Australia	HB3	ATGAACAGTCTATCCTCCTTTAG	147.33439
BC15870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88582	WGS84	BC15870	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15870_Mgig_Bot2012_COI	HB2	0	BC15870_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15870	Sample	BC15870	targeted	Absent	Sample	Mgig_Bot2012_COI	HB2	COI	45	BC15870	1.00E-05	Nil	Australia	HB2	TCTTATTCGTTGGAGACTTTATAACCCT	147.33562
BC15871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84429	WGS84	BC15871	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.745	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15871_Cmae_Bot2011_COI	HB19	0	BC15871_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15871	Sample	BC15871	targeted	Present	Sample	Cmae_Bot2011_COI	HB19	COI	45	BC15871	1.00E-05	Nil	Australia	HB19	ATGAACAGTCTATCCTCCTTTAG	147.33007
BC15873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88523	WGS84	BC15873	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.293	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15873_Aamu_Bax2006_COI	HB12	0	BC15873_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15873	Sample	BC15873	targeted	Present	Sample	Aamu_Bax2006_COI	HB12	COI	45	BC15873	1.00E-05	Nil	Australia	HB12	GCACAACCGGGATCTTTACTTC	147.34082
BC15873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88523	WGS84	BC15873	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15873_Sspa_Oph2007_COI	HB12	0	BC15873_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15873	Sample	BC15873	targeted	Absent	Sample	Sspa_Oph2007_COI	HB12	COI	45	BC15873	1.00E-05	Nil	Australia	HB12	GAAGGCCTTCCGATGATC	147.34082
BC15873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88523	WGS84	BC15873	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15873_Upin_Bot2011_COI	HB12	0	BC15873_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15873	Sample	BC15873	targeted	Absent	Sample	Upin_Bot2011_COI	HB12	COI	45	BC15873	1.00E-05	Nil	Australia	HB12	CTTTAATTACAGCGTTTTTATTGTTGT	147.34082
BC15874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88666	WGS84	BC15874	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15874_Mgig_Bot2012_COI	HB11	0	BC15874_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15874	Sample	BC15874	targeted	Absent	Sample	Mgig_Bot2012_COI	HB11	COI	45	BC15874	1.00E-05	Nil	Australia	HB11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33999
BC15875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8191	WGS84	BC15875	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15875_Cmae_Bot2011_COI	MC69	0	BC15875_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15875	Sample	BC15875	targeted	Absent	Sample	Cmae_Bot2011_COI	MC69	COI	45	BC15875	1.00E-05	Nil	Australia	MC69	ATGAACAGTCTATCCTCCTTTAG	144.92265
BC15877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82039	WGS84	BC15877	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.39	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15877_Aamu_Bax2006_COI	MD97	0	BC15877_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15877	Sample	BC15877	targeted	Present	Sample	Aamu_Bax2006_COI	MD97	COI	45	BC15877	1.00E-05	Nil	Australia	MD97	GCACAACCGGGATCTTTACTTC	144.91577
BC15877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82039	WGS84	BC15877	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.522	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15877_Sspa_Oph2007_COI	MD97	0	BC15877_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15877	Sample	BC15877	targeted	Present	Sample	Sspa_Oph2007_COI	MD97	COI	45	BC15877	1.00E-05	Nil	Australia	MD97	GAAGGCCTTCCGATGATC	144.91577
BC15877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82039	WGS84	BC15877	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15877_Upin_Bot2011_COI	MD97	0	BC15877_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15877	Sample	BC15877	targeted	Absent	Sample	Upin_Bot2011_COI	MD97	COI	45	BC15877	1.00E-05	Nil	Australia	MD97	CTTTAATTACAGCGTTTTTATTGTTGT	144.91577
BC15878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81277	WGS84	BC15878	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15878_Mgig_Bot2012_COI	MD92	0	BC15878_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15878	Sample	BC15878	targeted	Absent	Sample	Mgig_Bot2012_COI	MD92	COI	45	BC15878	1.00E-05	Nil	Australia	MD92	TCTTATTCGTTGGAGACTTTATAACCCT	144.91297
BC15879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81384	WGS84	BC15879	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15879_Cmae_Bot2011_COI	MD89	0	BC15879_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15879	Sample	BC15879	targeted	Absent	Sample	Cmae_Bot2011_COI	MD89	COI	45	BC15879	1.00E-05	Nil	Australia	MD89	ATGAACAGTCTATCCTCCTTTAG	144.91298
BC15880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81641	WGS84	BC15880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.235	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15880_Aamu_Bax2006_COI	MD87	0	BC15880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15880	Sample	BC15880	targeted	Present	Sample	Aamu_Bax2006_COI	MD87	COI	45	BC15880	1.00E-05	Nil	Australia	MD87	GCACAACCGGGATCTTTACTTC	144.91328
BC15880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81641	WGS84	BC15880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.389	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15880_Sspa_Oph2007_COI	MD87	0	BC15880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15880	Sample	BC15880	targeted	Present	Sample	Sspa_Oph2007_COI	MD87	COI	45	BC15880	1.00E-05	Nil	Australia	MD87	GAAGGCCTTCCGATGATC	144.91328
BC15880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81641	WGS84	BC15880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15880_Upin_Bot2011_COI	MD87	0	BC15880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15880	Sample	BC15880	targeted	Absent	Sample	Upin_Bot2011_COI	MD87	COI	45	BC15880	1.00E-05	Nil	Australia	MD87	CTTTAATTACAGCGTTTTTATTGTTGT	144.91328
BC15881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81912	WGS84	BC15881	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15881_Mgig_Bot2012_COI	MD84	0	BC15881_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15881	Sample	BC15881	targeted	Absent	Sample	Mgig_Bot2012_COI	MD84	COI	45	BC15881	1.00E-05	Nil	Australia	MD84	TCTTATTCGTTGGAGACTTTATAACCCT	144.9153
BC15882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15882	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15882_Cmae_Bot2011_COI	MD81	0	BC15882_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15882	Sample	BC15882	targeted	Absent	Sample	Cmae_Bot2011_COI	MD81	COI	45	BC15882	1.00E-05	Nil	Australia	MD81	ATGAACAGTCTATCCTCCTTTAG	144.9188
BC15884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81769	WGS84	BC15884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.097	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15884_Aamu_Bax2006_COI	MD78	0	BC15884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15884	Sample	BC15884	targeted	Present	Sample	Aamu_Bax2006_COI	MD78	COI	45	BC15884	1.00E-05	Nil	Australia	MD78	GCACAACCGGGATCTTTACTTC	144.92064
BC15884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81769	WGS84	BC15884	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.268	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15884_Sspa_Oph2007_COI	MD78	0	BC15884_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15884	Sample	BC15884	targeted	Present	Sample	Sspa_Oph2007_COI	MD78	COI	45	BC15884	1.00E-05	Nil	Australia	MD78	GAAGGCCTTCCGATGATC	144.92064
BC15884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81769	WGS84	BC15884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15884_Upin_Bot2011_COI	MD78	0	BC15884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15884	Sample	BC15884	targeted	Absent	Sample	Upin_Bot2011_COI	MD78	COI	45	BC15884	1.00E-05	Nil	Australia	MD78	CTTTAATTACAGCGTTTTTATTGTTGT	144.92064
BC15885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81826	WGS84	BC15885	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15885_Mgig_Bot2012_COI	MD76	0	BC15885_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15885	Sample	BC15885	targeted	Absent	Sample	Mgig_Bot2012_COI	MD76	COI	45	BC15885	1.00E-05	Nil	Australia	MD76	TCTTATTCGTTGGAGACTTTATAACCCT	144.92264
BC15886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81919	WGS84	BC15886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15886_Cmae_Bot2011_COI	MD72	0	BC15886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15886	Sample	BC15886	targeted	Absent	Sample	Cmae_Bot2011_COI	MD72	COI	45	BC15886	1.00E-05	Nil	Australia	MD72	ATGAACAGTCTATCCTCCTTTAG	144.92412
BC15888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81643	WGS84	BC15888	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.978	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15888_Aamu_Bax2006_COI	MD67	0	BC15888_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15888	Sample	BC15888	targeted	Present	Sample	Aamu_Bax2006_COI	MD67	COI	45	BC15888	1.00E-05	Nil	Australia	MD67	GCACAACCGGGATCTTTACTTC	144.92564
BC15888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81643	WGS84	BC15888	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.688	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15888_Sspa_Oph2007_COI	MD67	0	BC15888_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15888	Sample	BC15888	targeted	Present	Sample	Sspa_Oph2007_COI	MD67	COI	45	BC15888	1.00E-05	Nil	Australia	MD67	GAAGGCCTTCCGATGATC	144.92564
BC15888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81643	WGS84	BC15888	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15888_Upin_Bot2011_COI	MD67	0	BC15888_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15888	Sample	BC15888	targeted	Absent	Sample	Upin_Bot2011_COI	MD67	COI	45	BC15888	1.00E-05	Nil	Australia	MD67	CTTTAATTACAGCGTTTTTATTGTTGT	144.92564
BC15889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81561	WGS84	BC15889	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15889_Mgig_Bot2012_COI	MD66	0	BC15889_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15889	Sample	BC15889	targeted	Absent	Sample	Mgig_Bot2012_COI	MD66	COI	45	BC15889	1.00E-05	Nil	Australia	MD66	TCTTATTCGTTGGAGACTTTATAACCCT	144.92688
BC15891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81838	WGS84	BC15891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.34	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15891_Aamu_Bax2006_COI	MD61	0	BC15891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15891	Sample	BC15891	targeted	Present	Sample	Aamu_Bax2006_COI	MD61	COI	45	BC15891	1.00E-05	Nil	Australia	MD61	GCACAACCGGGATCTTTACTTC	144.92872
BC15891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81838	WGS84	BC15891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.767	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15891_Sspa_Oph2007_COI	MD61	0	BC15891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15891	Sample	BC15891	targeted	Present	Sample	Sspa_Oph2007_COI	MD61	COI	45	BC15891	1.00E-05	Nil	Australia	MD61	GAAGGCCTTCCGATGATC	144.92872
BC15891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81838	WGS84	BC15891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15891_Upin_Bot2011_COI	MD61	0	BC15891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15891	Sample	BC15891	targeted	Absent	Sample	Upin_Bot2011_COI	MD61	COI	45	BC15891	1.00E-05	Nil	Australia	MD61	CTTTAATTACAGCGTTTTTATTGTTGT	144.92872
BC15892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81935	WGS84	BC15892	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15892_Mgig_Bot2012_COI	MD58	0	BC15892_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15892	Sample	BC15892	targeted	Absent	Sample	Mgig_Bot2012_COI	MD58	COI	45	BC15892	1.00E-05	Nil	Australia	MD58	TCTTATTCGTTGGAGACTTTATAACCCT	144.92941
BC15893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15893	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15893_Cmae_Bot2011_COI	MD56	0	BC15893_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15893	Sample	BC15893	targeted	Absent	Sample	Cmae_Bot2011_COI	MD56	COI	45	BC15893	1.00E-05	Nil	Australia	MD56	ATGAACAGTCTATCCTCCTTTAG	144.93145
BC15895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85976	WGS84	BC15895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.686	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15895_Aamu_Bax2006_COI	MD52	0	BC15895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15895	Sample	BC15895	targeted	Present	Sample	Aamu_Bax2006_COI	MD52	COI	45	BC15895	1.00E-05	Nil	Australia	MD52	GCACAACCGGGATCTTTACTTC	144.91078
BC15895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85976	WGS84	BC15895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.829	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15895_Sspa_Oph2007_COI	MD52	0	BC15895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15895	Sample	BC15895	targeted	Present	Sample	Sspa_Oph2007_COI	MD52	COI	45	BC15895	1.00E-05	Nil	Australia	MD52	GAAGGCCTTCCGATGATC	144.91078
BC15895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85976	WGS84	BC15895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.839	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15895_Upin_Bot2011_COI	MD52	0	BC15895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15895	Sample	BC15895	targeted	Present	Sample	Upin_Bot2011_COI	MD52	COI	45	BC15895	1.00E-05	Nil	Australia	MD52	CTTTAATTACAGCGTTTTTATTGTTGT	144.91078
BC15896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86205	WGS84	BC15896	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15896_Mgig_Bot2012_COI	MD47	0	BC15896_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15896	Sample	BC15896	targeted	Absent	Sample	Mgig_Bot2012_COI	MD47	COI	45	BC15896	1.00E-05	Nil	Australia	MD47	TCTTATTCGTTGGAGACTTTATAACCCT	144.91494
BC15897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86128	WGS84	BC15897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15897_Cmae_Bot2011_COI	MD46	0	BC15897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15897	Sample	BC15897	targeted	Absent	Sample	Cmae_Bot2011_COI	MD46	COI	45	BC15897	1.00E-05	Nil	Australia	MD46	ATGAACAGTCTATCCTCCTTTAG	144.91286
BC15899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86256	WGS84	BC15899	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.246	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15899_Aamu_Bax2006_COI	MD41	0	BC15899_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15899	Sample	BC15899	targeted	Present	Sample	Aamu_Bax2006_COI	MD41	COI	45	BC15899	1.00E-05	Nil	Australia	MD41	GCACAACCGGGATCTTTACTTC	144.91611
BC15899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86256	WGS84	BC15899	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.714	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15899_Sspa_Oph2007_COI	MD41	0	BC15899_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15899	Sample	BC15899	targeted	Present	Sample	Sspa_Oph2007_COI	MD41	COI	45	BC15899	1.00E-05	Nil	Australia	MD41	GAAGGCCTTCCGATGATC	144.91611
BC15899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86256	WGS84	BC15899	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.854	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15899_Upin_Bot2011_COI	MD41	0	BC15899_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15899	Sample	BC15899	targeted	Present	Sample	Upin_Bot2011_COI	MD41	COI	45	BC15899	1.00E-05	Nil	Australia	MD41	CTTTAATTACAGCGTTTTTATTGTTGT	144.91611
BC15900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86111	WGS84	BC15900	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15900_Mgig_Bot2012_COI	MD34	0	BC15900_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15900	Sample	BC15900	targeted	Absent	Sample	Mgig_Bot2012_COI	MD34	COI	45	BC15900	1.00E-05	Nil	Australia	MD34	TCTTATTCGTTGGAGACTTTATAACCCT	144.90524
BC15901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86453	WGS84	BC15901	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15901_Cmae_Bot2011_COI	MD32	0	BC15901_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15901	Sample	BC15901	targeted	Absent	Sample	Cmae_Bot2011_COI	MD32	COI	45	BC15901	1.00E-05	Nil	Australia	MD32	ATGAACAGTCTATCCTCCTTTAG	144.91606
BC15903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86574	WGS84	BC15903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.722	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15903_Aamu_Bax2006_COI	MD29	0	BC15903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15903	Sample	BC15903	targeted	Present	Sample	Aamu_Bax2006_COI	MD29	COI	45	BC15903	1.00E-05	Nil	Australia	MD29	GCACAACCGGGATCTTTACTTC	144.91648
BC15903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86574	WGS84	BC15903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.326	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15903_Sspa_Oph2007_COI	MD29	0	BC15903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15903	Sample	BC15903	targeted	Present	Sample	Sspa_Oph2007_COI	MD29	COI	45	BC15903	1.00E-05	Nil	Australia	MD29	GAAGGCCTTCCGATGATC	144.91648
BC15903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86574	WGS84	BC15903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.867	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15903_Upin_Bot2011_COI	MD29	0	BC15903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15903	Sample	BC15903	targeted	Present	Sample	Upin_Bot2011_COI	MD29	COI	45	BC15903	1.00E-05	Nil	Australia	MD29	CTTTAATTACAGCGTTTTTATTGTTGT	144.91648
BC15904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85005	WGS84	BC15904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15904_Mgig_Bot2012_COI	MD27	0	BC15904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15904	Sample	BC15904	targeted	Absent	Sample	Mgig_Bot2012_COI	MD27	COI	45	BC15904	1.00E-05	Nil	Australia	MD27	TCTTATTCGTTGGAGACTTTATAACCCT	144.91302
BC15905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84831	WGS84	BC15905	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15905_Cmae_Bot2011_COI	MD24	0	BC15905_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15905	Sample	BC15905	targeted	Absent	Sample	Cmae_Bot2011_COI	MD24	COI	45	BC15905	1.00E-05	Nil	Australia	MD24	ATGAACAGTCTATCCTCCTTTAG	144.91069
BC15907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84561	WGS84	BC15907	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.33	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15907_Aamu_Bax2006_COI	MD18	0	BC15907_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15907	Sample	BC15907	targeted	Present	Sample	Aamu_Bax2006_COI	MD18	COI	45	BC15907	1.00E-05	Nil	Australia	MD18	GCACAACCGGGATCTTTACTTC	144.90755
BC15907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84561	WGS84	BC15907	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.523	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15907_Sspa_Oph2007_COI	MD18	0	BC15907_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15907	Sample	BC15907	targeted	Present	Sample	Sspa_Oph2007_COI	MD18	COI	45	BC15907	1.00E-05	Nil	Australia	MD18	GAAGGCCTTCCGATGATC	144.90755
BC15907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84561	WGS84	BC15907	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.062	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15907_Upin_Bot2011_COI	MD18	0	BC15907_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15907	Sample	BC15907	targeted	Present	Sample	Upin_Bot2011_COI	MD18	COI	45	BC15907	1.00E-05	Nil	Australia	MD18	CTTTAATTACAGCGTTTTTATTGTTGT	144.90755
BC15908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84019	WGS84	BC15908	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15908_Mgig_Bot2012_COI	MD13	0	BC15908_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15908	Sample	BC15908	targeted	Absent	Sample	Mgig_Bot2012_COI	MD13	COI	45	BC15908	1.00E-05	Nil	Australia	MD13	TCTTATTCGTTGGAGACTTTATAACCCT	144.90569
BC15909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84466	WGS84	BC15909	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15909_Cmae_Bot2011_COI	MD2	0	BC15909_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15909	Sample	BC15909	targeted	Absent	Sample	Cmae_Bot2011_COI	MD2	COI	45	BC15909	1.00E-05	Nil	Australia	MD2	ATGAACAGTCTATCCTCCTTTAG	144.93199
BC15910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82758	WGS84	BC15910	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.4	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15910_Aamu_Bax2006_COI	MD126	0	BC15910_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15910	Sample	BC15910	targeted	Present	Sample	Aamu_Bax2006_COI	MD126	COI	45	BC15910	1.00E-05	Nil	Australia	MD126	GCACAACCGGGATCTTTACTTC	144.89942
BC15910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82758	WGS84	BC15910	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.14	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15910_Sspa_Oph2007_COI	MD126	0	BC15910_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15910	Sample	BC15910	targeted	Present	Sample	Sspa_Oph2007_COI	MD126	COI	45	BC15910	1.00E-05	Nil	Australia	MD126	GAAGGCCTTCCGATGATC	144.89942
BC15910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82758	WGS84	BC15910	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15910_Upin_Bot2011_COI	MD126	0	BC15910_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15910	Sample	BC15910	targeted	Absent	Sample	Upin_Bot2011_COI	MD126	COI	45	BC15910	1.00E-05	Nil	Australia	MD126	CTTTAATTACAGCGTTTTTATTGTTGT	144.89942
BC15911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82593	WGS84	BC15911	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15911_Mgig_Bot2012_COI	MD122	0	BC15911_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15911	Sample	BC15911	targeted	Absent	Sample	Mgig_Bot2012_COI	MD122	COI	45	BC15911	1.00E-05	Nil	Australia	MD122	TCTTATTCGTTGGAGACTTTATAACCCT	144.90066
BC15912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82515	WGS84	BC15912	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15912_Cmae_Bot2011_COI	MD121	0	BC15912_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15912	Sample	BC15912	targeted	Absent	Sample	Cmae_Bot2011_COI	MD121	COI	45	BC15912	1.00E-05	Nil	Australia	MD121	ATGAACAGTCTATCCTCCTTTAG	144.90263
BC15914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8234	WGS84	BC15914	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.092	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15914_Aamu_Bax2006_COI	MD116	0	BC15914_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15914	Sample	BC15914	targeted	Present	Sample	Aamu_Bax2006_COI	MD116	COI	45	BC15914	1.00E-05	Nil	Australia	MD116	GCACAACCGGGATCTTTACTTC	144.906
BC15914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8234	WGS84	BC15914	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.876	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15914_Sspa_Oph2007_COI	MD116	0	BC15914_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15914	Sample	BC15914	targeted	Present	Sample	Sspa_Oph2007_COI	MD116	COI	45	BC15914	1.00E-05	Nil	Australia	MD116	GAAGGCCTTCCGATGATC	144.906
BC15914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8234	WGS84	BC15914	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15914_Upin_Bot2011_COI	MD116	0	BC15914_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15914	Sample	BC15914	targeted	Absent	Sample	Upin_Bot2011_COI	MD116	COI	45	BC15914	1.00E-05	Nil	Australia	MD116	CTTTAATTACAGCGTTTTTATTGTTGT	144.906
BC15915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82204	WGS84	BC15915	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15915_Mgig_Bot2012_COI	MD114	0	BC15915_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15915	Sample	BC15915	targeted	Absent	Sample	Mgig_Bot2012_COI	MD114	COI	45	BC15915	1.00E-05	Nil	Australia	MD114	TCTTATTCGTTGGAGACTTTATAACCCT	144.90544
BC15916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81945	WGS84	BC15916	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15916_Cmae_Bot2011_COI	MD112	0	BC15916_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15916	Sample	BC15916	targeted	Absent	Sample	Cmae_Bot2011_COI	MD112	COI	45	BC15916	1.00E-05	Nil	Australia	MD112	ATGAACAGTCTATCCTCCTTTAG	144.90621
BC15918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82047	WGS84	BC15918	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.241	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15918_Aamu_Bax2006_COI	MD103	0	BC15918_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15918	Sample	BC15918	targeted	Present	Sample	Aamu_Bax2006_COI	MD103	COI	45	BC15918	1.00E-05	Nil	Australia	MD103	GCACAACCGGGATCTTTACTTC	144.90914
BC15918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82047	WGS84	BC15918	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.84	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15918_Sspa_Oph2007_COI	MD103	0	BC15918_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15918	Sample	BC15918	targeted	Present	Sample	Sspa_Oph2007_COI	MD103	COI	45	BC15918	1.00E-05	Nil	Australia	MD103	GAAGGCCTTCCGATGATC	144.90914
BC15918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82047	WGS84	BC15918	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15918_Upin_Bot2011_COI	MD103	0	BC15918_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15918	Sample	BC15918	targeted	Absent	Sample	Upin_Bot2011_COI	MD103	COI	45	BC15918	1.00E-05	Nil	Australia	MD103	CTTTAATTACAGCGTTTTTATTGTTGT	144.90914
BC15919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82053	WGS84	BC15919	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15919_Mgig_Bot2012_COI	MD102	0	BC15919_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15919	Sample	BC15919	targeted	Absent	Sample	Mgig_Bot2012_COI	MD102	COI	45	BC15919	1.00E-05	Nil	Australia	MD102	TCTTATTCGTTGGAGACTTTATAACCCT	144.91301
BC15921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81914	WGS84	BC15921	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.851	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15921_Aamu_Bax2006_COI	MC97	0	BC15921_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15921	Sample	BC15921	targeted	Present	Sample	Aamu_Bax2006_COI	MC97	COI	45	BC15921	1.00E-05	Nil	Australia	MC97	GCACAACCGGGATCTTTACTTC	144.91473
BC15921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81914	WGS84	BC15921	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.637	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15921_Sspa_Oph2007_COI	MC97	0	BC15921_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15921	Sample	BC15921	targeted	Present	Sample	Sspa_Oph2007_COI	MC97	COI	45	BC15921	1.00E-05	Nil	Australia	MC97	GAAGGCCTTCCGATGATC	144.91473
BC15921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81914	WGS84	BC15921	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15921_Upin_Bot2011_COI	MC97	0	BC15921_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15921	Sample	BC15921	targeted	Absent	Sample	Upin_Bot2011_COI	MC97	COI	45	BC15921	1.00E-05	Nil	Australia	MC97	CTTTAATTACAGCGTTTTTATTGTTGT	144.91473
BC15922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81548	WGS84	BC15922	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.941	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15922_Mgig_Bot2012_COI	MC93	0	BC15922_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15922	Sample	BC15922	targeted	Present	Sample	Mgig_Bot2012_COI	MC93	COI	45	BC15922	1.00E-05	Nil	Australia	MC93	TCTTATTCGTTGGAGACTTTATAACCCT	144.91301
BC15923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81339	WGS84	BC15923	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15923_Cmae_Bot2011_COI	MC92	0	BC15923_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15923	Sample	BC15923	targeted	Absent	Sample	Cmae_Bot2011_COI	MC92	COI	45	BC15923	1.00E-05	Nil	Australia	MC92	ATGAACAGTCTATCCTCCTTTAG	144.91286
BC15925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.818	WGS84	BC15925	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15925_Aamu_Bax2006_COI	MC84	0	BC15925_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15925	Sample	BC15925	targeted	Absent	Sample	Aamu_Bax2006_COI	MC84	COI	45	BC15925	1.00E-05	Nil	Australia	MC84	GCACAACCGGGATCTTTACTTC	144.92319
BC15925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.818	WGS84	BC15925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.924	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15925_Sspa_Oph2007_COI	MC84	0	BC15925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15925	Sample	BC15925	targeted	Present	Sample	Sspa_Oph2007_COI	MC84	COI	45	BC15925	1.00E-05	Nil	Australia	MC84	GAAGGCCTTCCGATGATC	144.92319
BC15925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.818	WGS84	BC15925	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15925_Upin_Bot2011_COI	MC84	0	BC15925_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15925	Sample	BC15925	targeted	Absent	Sample	Upin_Bot2011_COI	MC84	COI	45	BC15925	1.00E-05	Nil	Australia	MC84	CTTTAATTACAGCGTTTTTATTGTTGT	144.92319
BC15926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81972	WGS84	BC15926	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.687	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15926_Mgig_Bot2012_COI	MC83	0	BC15926_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15926	Sample	BC15926	targeted	Present	Sample	Mgig_Bot2012_COI	MC83	COI	45	BC15926	1.00E-05	Nil	Australia	MC83	TCTTATTCGTTGGAGACTTTATAACCCT	144.92466
BC15927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84373	WGS84	BC15927	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15927_Cmae_Bot2011_COI	MC8	0	BC15927_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15927	Sample	BC15927	targeted	Absent	Sample	Cmae_Bot2011_COI	MC8	COI	45	BC15927	1.00E-05	Nil	Australia	MC8	ATGAACAGTCTATCCTCCTTTAG	144.92868
BC15929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81951	WGS84	BC15929	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15929_Aamu_Bax2006_COI	MC76	0	BC15929_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15929	Sample	BC15929	targeted	Absent	Sample	Aamu_Bax2006_COI	MC76	COI	45	BC15929	1.00E-05	Nil	Australia	MC76	GCACAACCGGGATCTTTACTTC	144.92794
BC15929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81951	WGS84	BC15929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.673	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15929_Sspa_Oph2007_COI	MC76	0	BC15929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15929	Sample	BC15929	targeted	Present	Sample	Sspa_Oph2007_COI	MC76	COI	45	BC15929	1.00E-05	Nil	Australia	MC76	GAAGGCCTTCCGATGATC	144.92794
BC15929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81951	WGS84	BC15929	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15929_Upin_Bot2011_COI	MC76	0	BC15929_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15929	Sample	BC15929	targeted	Absent	Sample	Upin_Bot2011_COI	MC76	COI	45	BC15929	1.00E-05	Nil	Australia	MC76	CTTTAATTACAGCGTTTTTATTGTTGT	144.92794
BC15930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81842	WGS84	BC15930	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15930_Cmae_Bot2011_COI	MC74	0	BC15930_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15930	Sample	BC15930	targeted	Absent	Sample	Cmae_Bot2011_COI	MC74	COI	45	BC15930	1.00E-05	Nil	Australia	MC74	ATGAACAGTCTATCCTCCTTTAG	144.92982
BC15932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81869	WGS84	BC15932	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15932_Aamu_Bax2006_COI	MC67	0	BC15932_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15932	Sample	BC15932	targeted	Absent	Sample	Aamu_Bax2006_COI	MC67	COI	45	BC15932	1.00E-05	Nil	Australia	MC67	GCACAACCGGGATCTTTACTTC	144.91994
BC15932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81869	WGS84	BC15932	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.546	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15932_Sspa_Oph2007_COI	MC67	0	BC15932_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15932	Sample	BC15932	targeted	Present	Sample	Sspa_Oph2007_COI	MC67	COI	45	BC15932	1.00E-05	Nil	Australia	MC67	GAAGGCCTTCCGATGATC	144.91994
BC15932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81869	WGS84	BC15932	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15932_Upin_Bot2011_COI	MC67	0	BC15932_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15932	Sample	BC15932	targeted	Absent	Sample	Upin_Bot2011_COI	MC67	COI	45	BC15932	1.00E-05	Nil	Australia	MC67	CTTTAATTACAGCGTTTTTATTGTTGT	144.91994
BC15933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81948	WGS84	BC15933	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.709	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15933_Mgig_Bot2012_COI	MC63	0	BC15933_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15933	Sample	BC15933	targeted	Present	Sample	Mgig_Bot2012_COI	MC63	COI	45	BC15933	1.00E-05	Nil	Australia	MC63	TCTTATTCGTTGGAGACTTTATAACCCT	144.91605
BC15934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82262	WGS84	BC15934	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15934_Cmae_Bot2011_COI	MC61	0	BC15934_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15934	Sample	BC15934	targeted	Absent	Sample	Cmae_Bot2011_COI	MC61	COI	45	BC15934	1.00E-05	Nil	Australia	MC61	ATGAACAGTCTATCCTCCTTTAG	144.90604
BC15936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82372	WGS84	BC15936	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15936_Aamu_Bax2006_COI	MC59	0	BC15936_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15936	Sample	BC15936	targeted	Absent	Sample	Aamu_Bax2006_COI	MC59	COI	45	BC15936	1.00E-05	Nil	Australia	MC59	GCACAACCGGGATCTTTACTTC	144.90481
BC15936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82372	WGS84	BC15936	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.399	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15936_Sspa_Oph2007_COI	MC59	0	BC15936_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15936	Sample	BC15936	targeted	Present	Sample	Sspa_Oph2007_COI	MC59	COI	45	BC15936	1.00E-05	Nil	Australia	MC59	GAAGGCCTTCCGATGATC	144.90481
BC15936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82372	WGS84	BC15936	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15936_Upin_Bot2011_COI	MC59	0	BC15936_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15936	Sample	BC15936	targeted	Absent	Sample	Upin_Bot2011_COI	MC59	COI	45	BC15936	1.00E-05	Nil	Australia	MC59	CTTTAATTACAGCGTTTTTATTGTTGT	144.90481
BC15937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82384	WGS84	BC15937	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.905	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15937_Mgig_Bot2012_COI	MC58	0	BC15937_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15937	Sample	BC15937	targeted	Present	Sample	Mgig_Bot2012_COI	MC58	COI	45	BC15937	1.00E-05	Nil	Australia	MC58	TCTTATTCGTTGGAGACTTTATAACCCT	144.90353
BC15938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82375	WGS84	BC15938	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15938_Cmae_Bot2011_COI	MC57	0	BC15938_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15938	Sample	BC15938	targeted	Absent	Sample	Cmae_Bot2011_COI	MC57	COI	45	BC15938	1.00E-05	Nil	Australia	MC57	ATGAACAGTCTATCCTCCTTTAG	144.90284
BC15940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82722	WGS84	BC15940	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.128	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15940_Mgig_Bot2012_COI	MC51	0	BC15940_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15940	Sample	BC15940	targeted	Present	Sample	Mgig_Bot2012_COI	MC51	COI	45	BC15940	1.00E-05	Nil	Australia	MC51	TCTTATTCGTTGGAGACTTTATAACCCT	144.90064
BC15941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82723	WGS84	BC15941	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15941_Cmae_Bot2011_COI	MC49	0	BC15941_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15941	Sample	BC15941	targeted	Absent	Sample	Cmae_Bot2011_COI	MC49	COI	45	BC15941	1.00E-05	Nil	Australia	MC49	ATGAACAGTCTATCCTCCTTTAG	144.9018
BC15943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8442	WGS84	BC15943	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15943_Aamu_Bax2006_COI	MC3	0	BC15943_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15943	Sample	BC15943	targeted	Absent	Sample	Aamu_Bax2006_COI	MC3	COI	45	BC15943	1.00E-05	Nil	Australia	MC3	GCACAACCGGGATCTTTACTTC	144.92999
BC15943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8442	WGS84	BC15943	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.825	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15943_Sspa_Oph2007_COI	MC3	0	BC15943_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15943	Sample	BC15943	targeted	Present	Sample	Sspa_Oph2007_COI	MC3	COI	45	BC15943	1.00E-05	Nil	Australia	MC3	GAAGGCCTTCCGATGATC	144.92999
BC15943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8442	WGS84	BC15943	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15943_Upin_Bot2011_COI	MC3	0	BC15943_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15943	Sample	BC15943	targeted	Absent	Sample	Upin_Bot2011_COI	MC3	COI	45	BC15943	1.00E-05	Nil	Australia	MC3	CTTTAATTACAGCGTTTTTATTGTTGT	144.92999
BC15944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84007	WGS84	BC15944	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.332	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15944_Mgig_Bot2012_COI	MC28	0	BC15944_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15944	Sample	BC15944	targeted	Present	Sample	Mgig_Bot2012_COI	MC28	COI	45	BC15944	1.00E-05	Nil	Australia	MC28	TCTTATTCGTTGGAGACTTTATAACCCT	144.90559
BC15945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84115	WGS84	BC15945	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15945_Cmae_Bot2011_COI	MC27	0	BC15945_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15945	Sample	BC15945	targeted	Absent	Sample	Cmae_Bot2011_COI	MC27	COI	45	BC15945	1.00E-05	Nil	Australia	MC27	ATGAACAGTCTATCCTCCTTTAG	144.90495
BC15947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84617	WGS84	BC15947	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15947_Aamu_Bax2006_COI	MC21	0	BC15947_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15947	Sample	BC15947	targeted	Absent	Sample	Aamu_Bax2006_COI	MC21	COI	45	BC15947	1.00E-05	Nil	Australia	MC21	GCACAACCGGGATCTTTACTTC	144.90876
BC15947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84617	WGS84	BC15947	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.619	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15947_Sspa_Oph2007_COI	MC21	0	BC15947_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15947	Sample	BC15947	targeted	Present	Sample	Sspa_Oph2007_COI	MC21	COI	45	BC15947	1.00E-05	Nil	Australia	MC21	GAAGGCCTTCCGATGATC	144.90876
BC15947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84617	WGS84	BC15947	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15947_Upin_Bot2011_COI	MC21	0	BC15947_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15947	Sample	BC15947	targeted	Absent	Sample	Upin_Bot2011_COI	MC21	COI	45	BC15947	1.00E-05	Nil	Australia	MC21	CTTTAATTACAGCGTTTTTATTGTTGT	144.90876
BC15948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84791	WGS84	BC15948	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.444	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15948_Mgig_Bot2012_COI	MC19	0	BC15948_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15948	Sample	BC15948	targeted	Present	Sample	Mgig_Bot2012_COI	MC19	COI	45	BC15948	1.00E-05	Nil	Australia	MC19	TCTTATTCGTTGGAGACTTTATAACCCT	144.90758
BC15949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84786	WGS84	BC15949	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15949_Cmae_Bot2011_COI	MC18	0	BC15949_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15949	Sample	BC15949	targeted	Absent	Sample	Cmae_Bot2011_COI	MC18	COI	45	BC15949	1.00E-05	Nil	Australia	MC18	ATGAACAGTCTATCCTCCTTTAG	144.90858
BC15950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8496	WGS84	BC15950	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15950_Aamu_Bax2006_COI	MC14	0	BC15950_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15950	Sample	BC15950	targeted	Absent	Sample	Aamu_Bax2006_COI	MC14	COI	45	BC15950	1.00E-05	Nil	Australia	MC14	GCACAACCGGGATCTTTACTTC	144.90892
BC15950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8496	WGS84	BC15950	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.756	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15950_Sspa_Oph2007_COI	MC14	0	BC15950_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15950	Sample	BC15950	targeted	Present	Sample	Sspa_Oph2007_COI	MC14	COI	45	BC15950	1.00E-05	Nil	Australia	MC14	GAAGGCCTTCCGATGATC	144.90892
BC15950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8496	WGS84	BC15950	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15950_Upin_Bot2011_COI	MC14	0	BC15950_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15950	Sample	BC15950	targeted	Absent	Sample	Upin_Bot2011_COI	MC14	COI	45	BC15950	1.00E-05	Nil	Australia	MC14	CTTTAATTACAGCGTTTTTATTGTTGT	144.90892
BC15951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85836	WGS84	BC15951	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.675	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15951_Mgig_Bot2012_COI	MC122	0	BC15951_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15951	Sample	BC15951	targeted	Present	Sample	Mgig_Bot2012_COI	MC122	COI	45	BC15951	1.00E-05	Nil	Australia	MC122	TCTTATTCGTTGGAGACTTTATAACCCT	144.90732
BC15952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82163	WGS84	BC15952	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15952_Cmae_Bot2011_COI	MC116	0	BC15952_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15952	Sample	BC15952	targeted	Absent	Sample	Cmae_Bot2011_COI	MC116	COI	45	BC15952	1.00E-05	Nil	Australia	MC116	ATGAACAGTCTATCCTCCTTTAG	144.90604
BC15954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82137	WGS84	BC15954	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15954_Aamu_Bax2006_COI	MC113	0	BC15954_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15954	Sample	BC15954	targeted	Absent	Sample	Aamu_Bax2006_COI	MC113	COI	45	BC15954	1.00E-05	Nil	Australia	MC113	GCACAACCGGGATCTTTACTTC	144.91095
BC15954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82137	WGS84	BC15954	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.296	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15954_Sspa_Oph2007_COI	MC113	0	BC15954_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15954	Sample	BC15954	targeted	Present	Sample	Sspa_Oph2007_COI	MC113	COI	45	BC15954	1.00E-05	Nil	Australia	MC113	GAAGGCCTTCCGATGATC	144.91095
BC15954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82137	WGS84	BC15954	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15954_Upin_Bot2011_COI	MC113	0	BC15954_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15954	Sample	BC15954	targeted	Absent	Sample	Upin_Bot2011_COI	MC113	COI	45	BC15954	1.00E-05	Nil	Australia	MC113	CTTTAATTACAGCGTTTTTATTGTTGT	144.91095
BC15955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82293	WGS84	BC15955	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.921	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15955_Mgig_Bot2012_COI	MC112	0	BC15955_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15955	Sample	BC15955	targeted	Present	Sample	Mgig_Bot2012_COI	MC112	COI	45	BC15955	1.00E-05	Nil	Australia	MC112	TCTTATTCGTTGGAGACTTTATAACCCT	144.90727
BC15956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81995	WGS84	BC15956	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15956_Cmae_Bot2011_COI	MC111	0	BC15956_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15956	Sample	BC15956	targeted	Absent	Sample	Cmae_Bot2011_COI	MC111	COI	45	BC15956	1.00E-05	Nil	Australia	MC111	ATGAACAGTCTATCCTCCTTTAG	144.91018
BC15958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81946	WGS84	BC15958	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15958_Aamu_Bax2006_COI	MC109	0	BC15958_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15958	Sample	BC15958	targeted	Absent	Sample	Aamu_Bax2006_COI	MC109	COI	45	BC15958	1.00E-05	Nil	Australia	MC109	GCACAACCGGGATCTTTACTTC	144.91081
BC15958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81946	WGS84	BC15958	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.234	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15958_Sspa_Oph2007_COI	MC109	0	BC15958_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15958	Sample	BC15958	targeted	Present	Sample	Sspa_Oph2007_COI	MC109	COI	45	BC15958	1.00E-05	Nil	Australia	MC109	GAAGGCCTTCCGATGATC	144.91081
BC15958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81946	WGS84	BC15958	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15958_Upin_Bot2011_COI	MC109	0	BC15958_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15958	Sample	BC15958	targeted	Absent	Sample	Upin_Bot2011_COI	MC109	COI	45	BC15958	1.00E-05	Nil	Australia	MC109	CTTTAATTACAGCGTTTTTATTGTTGT	144.91081
BC15959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81921	WGS84	BC15959	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.041	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15959_Mgig_Bot2012_COI	MC103	0	BC15959_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15959	Sample	BC15959	targeted	Present	Sample	Mgig_Bot2012_COI	MC103	COI	45	BC15959	1.00E-05	Nil	Australia	MC103	TCTTATTCGTTGGAGACTTTATAACCCT	144.92594
BC15961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.80951	WGS84	BC15961	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15961_Aamu_Bax2006_COI	MB97	0	BC15961_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15961	Sample	BC15961	targeted	Absent	Sample	Aamu_Bax2006_COI	MB97	COI	45	BC15961	1.00E-05	Nil	Australia	MB97	GCACAACCGGGATCTTTACTTC	144.91335
BC15961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.80951	WGS84	BC15961	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.989	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15961_Sspa_Oph2007_COI	MB97	0	BC15961_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15961	Sample	BC15961	targeted	Present	Sample	Sspa_Oph2007_COI	MB97	COI	45	BC15961	1.00E-05	Nil	Australia	MB97	GAAGGCCTTCCGATGATC	144.91335
BC15961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.80951	WGS84	BC15961	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15961_Upin_Bot2011_COI	MB97	0	BC15961_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15961	Sample	BC15961	targeted	Absent	Sample	Upin_Bot2011_COI	MB97	COI	45	BC15961	1.00E-05	Nil	Australia	MB97	CTTTAATTACAGCGTTTTTATTGTTGT	144.91335
BC15962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81351	WGS84	BC15962	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.711	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15962_Mgig_Bot2012_COI	MB93	0	BC15962_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15962	Sample	BC15962	targeted	Present	Sample	Mgig_Bot2012_COI	MB93	COI	45	BC15962	1.00E-05	Nil	Australia	MB93	TCTTATTCGTTGGAGACTTTATAACCCT	144.91416
BC15963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81548	WGS84	BC15963	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15963_Cmae_Bot2011_COI	MB91	0	BC15963_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15963	Sample	BC15963	targeted	Absent	Sample	Cmae_Bot2011_COI	MB91	COI	45	BC15963	1.00E-05	Nil	Australia	MB91	ATGAACAGTCTATCCTCCTTTAG	144.9145
BC15965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81788	WGS84	BC15965	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.669	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15965_Aamu_Bax2006_COI	MB86	0	BC15965_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15965	Sample	BC15965	targeted	Present	Sample	Aamu_Bax2006_COI	MB86	COI	45	BC15965	1.00E-05	Nil	Australia	MB86	GCACAACCGGGATCTTTACTTC	144.91938
BC15965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81788	WGS84	BC15965	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.178	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15965_Sspa_Oph2007_COI	MB86	0	BC15965_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15965	Sample	BC15965	targeted	Present	Sample	Sspa_Oph2007_COI	MB86	COI	45	BC15965	1.00E-05	Nil	Australia	MB86	GAAGGCCTTCCGATGATC	144.91938
BC15965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81788	WGS84	BC15965	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15965_Upin_Bot2011_COI	MB86	0	BC15965_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15965	Sample	BC15965	targeted	Absent	Sample	Upin_Bot2011_COI	MB86	COI	45	BC15965	1.00E-05	Nil	Australia	MB86	CTTTAATTACAGCGTTTTTATTGTTGT	144.91938
BC15966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81667	WGS84	BC15966	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15966_Mgig_Bot2012_COI	MB84	0	BC15966_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15966	Sample	BC15966	targeted	Absent	Sample	Mgig_Bot2012_COI	MB84	COI	45	BC15966	1.00E-05	Nil	Australia	MB84	TCTTATTCGTTGGAGACTTTATAACCCT	144.92252
BC15967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81564	WGS84	BC15967	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15967_Cmae_Bot2011_COI	MB82	0	BC15967_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15967	Sample	BC15967	targeted	Absent	Sample	Cmae_Bot2011_COI	MB82	COI	45	BC15967	1.00E-05	Nil	Australia	MB82	ATGAACAGTCTATCCTCCTTTAG	144.92523
BC15969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81593	WGS84	BC15969	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15969_Aamu_Bax2006_COI	MB79	0	BC15969_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15969	Sample	BC15969	targeted	Absent	Sample	Aamu_Bax2006_COI	MB79	COI	45	BC15969	1.00E-05	Nil	Australia	MB79	GCACAACCGGGATCTTTACTTC	144.92751
BC15969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81593	WGS84	BC15969	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.448	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15969_Sspa_Oph2007_COI	MB79	0	BC15969_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15969	Sample	BC15969	targeted	Present	Sample	Sspa_Oph2007_COI	MB79	COI	45	BC15969	1.00E-05	Nil	Australia	MB79	GAAGGCCTTCCGATGATC	144.92751
BC15969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81593	WGS84	BC15969	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15969_Upin_Bot2011_COI	MB79	0	BC15969_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15969	Sample	BC15969	targeted	Absent	Sample	Upin_Bot2011_COI	MB79	COI	45	BC15969	1.00E-05	Nil	Australia	MB79	CTTTAATTACAGCGTTTTTATTGTTGT	144.92751
BC15970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81794	WGS84	BC15970	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15970_Cmae_Bot2011_COI	MB74	0	BC15970_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15970	Sample	BC15970	targeted	Absent	Sample	Cmae_Bot2011_COI	MB74	COI	45	BC15970	1.00E-05	Nil	Australia	MB74	ATGAACAGTCTATCCTCCTTTAG	144.93041
BC15972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81981	WGS84	BC15972	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15972_Aamu_Bax2006_COI	MB64	0	BC15972_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15972	Sample	BC15972	targeted	Absent	Sample	Aamu_Bax2006_COI	MB64	COI	45	BC15972	1.00E-05	Nil	Australia	MB64	GCACAACCGGGATCTTTACTTC	144.92026
BC15972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81981	WGS84	BC15972	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.201	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15972_Sspa_Oph2007_COI	MB64	0	BC15972_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15972	Sample	BC15972	targeted	Present	Sample	Sspa_Oph2007_COI	MB64	COI	45	BC15972	1.00E-05	Nil	Australia	MB64	GAAGGCCTTCCGATGATC	144.92026
BC15972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81981	WGS84	BC15972	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15972_Upin_Bot2011_COI	MB64	0	BC15972_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15972	Sample	BC15972	targeted	Absent	Sample	Upin_Bot2011_COI	MB64	COI	45	BC15972	1.00E-05	Nil	Australia	MB64	CTTTAATTACAGCGTTTTTATTGTTGT	144.92026
BC15973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84301	WGS84	BC15973	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15973_Mgig_Bot2012_COI	MB6	0	BC15973_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15973	Sample	BC15973	targeted	Absent	Sample	Mgig_Bot2012_COI	MB6	COI	45	BC15973	1.00E-05	Nil	Australia	MB6	TCTTATTCGTTGGAGACTTTATAACCCT	144.90526
BC15974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82323	WGS84	BC15974	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15974_Cmae_Bot2011_COI	MB59	0	BC15974_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15974	Sample	BC15974	targeted	Absent	Sample	Cmae_Bot2011_COI	MB59	COI	45	BC15974	1.00E-05	Nil	Australia	MB59	ATGAACAGTCTATCCTCCTTTAG	144.90667
BC15976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85669	WGS84	BC15976	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15976_Aamu_Bax2006_COI	MB53	0	BC15976_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15976	Sample	BC15976	targeted	Absent	Sample	Aamu_Bax2006_COI	MB53	COI	45	BC15976	1.00E-05	Nil	Australia	MB53	GCACAACCGGGATCTTTACTTC	144.90554
BC15976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85669	WGS84	BC15976	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.842	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15976_Sspa_Oph2007_COI	MB53	0	BC15976_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15976	Sample	BC15976	targeted	Present	Sample	Sspa_Oph2007_COI	MB53	COI	45	BC15976	1.00E-05	Nil	Australia	MB53	GAAGGCCTTCCGATGATC	144.90554
BC15976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85669	WGS84	BC15976	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.702	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15976_Upin_Bot2011_COI	MB53	0	BC15976_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15976	Sample	BC15976	targeted	Present	Sample	Upin_Bot2011_COI	MB53	COI	45	BC15976	1.00E-05	Nil	Australia	MB53	CTTTAATTACAGCGTTTTTATTGTTGT	144.90554
BC15977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85954	WGS84	BC15977	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15977_Mgig_Bot2012_COI	MB51	0	BC15977_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15977	Sample	BC15977	targeted	Absent	Sample	Mgig_Bot2012_COI	MB51	COI	45	BC15977	1.00E-05	Nil	Australia	MB51	TCTTATTCGTTGGAGACTTTATAACCCT	144.90766
BC15978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86119	WGS84	BC15978	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15978_Cmae_Bot2011_COI	MB48	0	BC15978_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15978	Sample	BC15978	targeted	Absent	Sample	Cmae_Bot2011_COI	MB48	COI	45	BC15978	1.00E-05	Nil	Australia	MB48	ATGAACAGTCTATCCTCCTTTAG	144.90855
BC15980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86182	WGS84	BC15980	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15980_Mgig_Bot2012_COI	MB42	0	BC15980_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15980	Sample	BC15980	targeted	Absent	Sample	Mgig_Bot2012_COI	MB42	COI	45	BC15980	1.00E-05	Nil	Australia	MB42	TCTTATTCGTTGGAGACTTTATAACCCT	144.91412
BC15981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8626	WGS84	BC15981	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15981_Cmae_Bot2011_COI	MB41	0	BC15981_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15981	Sample	BC15981	targeted	Absent	Sample	Cmae_Bot2011_COI	MB41	COI	45	BC15981	1.00E-05	Nil	Australia	MB41	ATGAACAGTCTATCCTCCTTTAG	144.91343
BC15983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8632	WGS84	BC15983	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.368	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15983_Aamu_Bax2006_COI	MB37	0	BC15983_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15983	Sample	BC15983	targeted	Present	Sample	Aamu_Bax2006_COI	MB37	COI	45	BC15983	1.00E-05	Nil	Australia	MB37	GCACAACCGGGATCTTTACTTC	144.91774
BC15983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8632	WGS84	BC15983	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.97	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15983_Sspa_Oph2007_COI	MB37	0	BC15983_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15983	Sample	BC15983	targeted	Present	Sample	Sspa_Oph2007_COI	MB37	COI	45	BC15983	1.00E-05	Nil	Australia	MB37	GAAGGCCTTCCGATGATC	144.91774
BC15983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8632	WGS84	BC15983	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.451	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15983_Upin_Bot2011_COI	MB37	0	BC15983_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15983	Sample	BC15983	targeted	Present	Sample	Upin_Bot2011_COI	MB37	COI	45	BC15983	1.00E-05	Nil	Australia	MB37	CTTTAATTACAGCGTTTTTATTGTTGT	144.91774
BC15984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86568	WGS84	BC15984	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15984_Mgig_Bot2012_COI	MB36	0	BC15984_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15984	Sample	BC15984	targeted	Absent	Sample	Mgig_Bot2012_COI	MB36	COI	45	BC15984	1.00E-05	Nil	Australia	MB36	TCTTATTCGTTGGAGACTTTATAACCCT	144.91795
BC15985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86493	WGS84	BC15985	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15985_Cmae_Bot2011_COI	MB34	0	BC15985_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15985	Sample	BC15985	targeted	Absent	Sample	Cmae_Bot2011_COI	MB34	COI	45	BC15985	1.00E-05	Nil	Australia	MB34	ATGAACAGTCTATCCTCCTTTAG	144.91762
BC15987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84065	WGS84	BC15987	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.13	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15987_Aamu_Bax2006_COI	MB3	0	BC15987_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15987	Sample	BC15987	targeted	Present	Sample	Aamu_Bax2006_COI	MB3	COI	45	BC15987	1.00E-05	Nil	Australia	MB3	GCACAACCGGGATCTTTACTTC	144.90472
BC15987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84065	WGS84	BC15987	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.122	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15987_Sspa_Oph2007_COI	MB3	0	BC15987_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15987	Sample	BC15987	targeted	Present	Sample	Sspa_Oph2007_COI	MB3	COI	45	BC15987	1.00E-05	Nil	Australia	MB3	GAAGGCCTTCCGATGATC	144.90472
BC15987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84065	WGS84	BC15987	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.832	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15987_Upin_Bot2011_COI	MB3	0	BC15987_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15987	Sample	BC15987	targeted	Present	Sample	Upin_Bot2011_COI	MB3	COI	45	BC15987	1.00E-05	Nil	Australia	MB3	CTTTAATTACAGCGTTTTTATTGTTGT	144.90472
BC15988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86288	WGS84	BC15988	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15988_Mgig_Bot2012_COI	MB29	0	BC15988_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15988	Sample	BC15988	targeted	Absent	Sample	Mgig_Bot2012_COI	MB29	COI	45	BC15988	1.00E-05	Nil	Australia	MB29	TCTTATTCGTTGGAGACTTTATAACCCT	144.91667
BC25830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.81013	WGS84	BC25830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.003	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25830_Cmae_Bot2011_COI	T18-04	1	BC25830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25830	Sample	BC25830	targeted	Present	Sample	Cmae_Bot2011_COI	T18-04	COI	45	BC25830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-04	ATGAACAGTCTATCCTCCTTTAG	147.50249
BC25832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25832_Aamu_Bax2006_COI	T18-06	1	BC25832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25832	Sample	BC25832	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-06	COI	45	BC25832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-06	GCACAACCGGGATCTTTACTTC	147.48547
BC25832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25832_Pcan_Bot2011_IGS	T18-06	1	BC25832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25832	Sample	BC25832	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-06	IGS	45	BC25832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-06	CGTAATCCTCAGTACTGGCTA	147.48547
BC25832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25832_Sspa_Oph2007_COI	T18-06	1	BC25832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25832	Sample	BC25832	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-06	COI	45	BC25832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-06	GAAGGCCTTCCGATGATC	147.48547
BC25832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25832_Upin_Bot2011_COI	T18-06	1	BC25832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25832	Sample	BC25832	targeted	Absent	Sample	Upin_Bot2011_COI	T18-06	COI	45	BC25832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.48547
BC25833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80019	WGS84	BC25833	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25833_Cjap_Sim2018_COI	T18-07	1	BC25833_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25833	Sample	BC25833	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-07	COI	45	BC25833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.46497
BC25833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80019	WGS84	BC25833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.207	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	28/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25833_Mgig_Bot2012_COI	T18-07	1	BC25833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25833	Sample	BC25833	targeted	Present	Sample	Mgig_Bot2012_COI	T18-07	COI	45	BC25833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.46497
BC25836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95973	WGS84	BC25836	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.649	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25836_Aamu_Bax2006_COI	T18-13	1	BC25836_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25836	Sample	BC25836	targeted	Present	Sample	Aamu_Bax2006_COI	T18-13	COI	45	BC25836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-13	GCACAACCGGGATCTTTACTTC	147.52782
BC25836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95973	WGS84	BC25836	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25836_Pcan_Bot2011_IGS	T18-13	1	BC25836_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25836	Sample	BC25836	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-13	IGS	45	BC25836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-13	CGTAATCCTCAGTACTGGCTA	147.52782
BC25836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95973	WGS84	BC25836	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25836_Sspa_Oph2007_COI	T18-13	1	BC25836_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25836	Sample	BC25836	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-13	COI	45	BC25836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-13	GAAGGCCTTCCGATGATC	147.52782
BC25836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95973	WGS84	BC25836	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25836_Upin_Bot2011_COI	T18-13	1	BC25836_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25836	Sample	BC25836	targeted	Absent	Sample	Upin_Bot2011_COI	T18-13	COI	45	BC25836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.52782
BC25837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25837	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25837_Cjap_Sim2018_COI	T18-14	1	BC25837_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25837	Sample	BC25837	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-14	COI	45	BC25837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52367
BC25837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25837	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.871	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	14/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25837_Mgig_Bot2012_COI	T18-14	1	BC25837_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25837	Sample	BC25837	targeted	Present	Sample	Mgig_Bot2012_COI	T18-14	COI	45	BC25837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.52367
BC25838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25838	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.692	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25838_Cmae_Bot2011_COI	T18-15	1	BC25838_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25838	Sample	BC25838	targeted	Present	Sample	Cmae_Bot2011_COI	T18-15	COI	45	BC25838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-15	ATGAACAGTCTATCCTCCTTTAG	147.52367
BC25840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25840	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25840_Cjap_Sim2018_COI	T18-17	1	BC25840_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25840	Sample	BC25840	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-17	COI	45	BC25840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.48547
BC25840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25840	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.972	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	28/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25840_Mgig_Bot2012_COI	T18-17	1	BC25840_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25840	Sample	BC25840	targeted	Present	Sample	Mgig_Bot2012_COI	T18-17	COI	45	BC25840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-17	TCTTATTCGTTGGAGACTTTATAACCCT	147.48547
BC25841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84676	WGS84	BC25841	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25841_Cmae_Bot2011_COI	S13-01	1	BC25841_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25841	Sample	BC25841	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-01	COI	45	BC25841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-01	ATGAACAGTCTATCCTCCTTTAG	138.49723
BC25843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84521	WGS84	BC25843	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25843_Aamu_Bax2006_COI	S13-03	1	BC25843_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25843	Sample	BC25843	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-03	COI	45	BC25843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-03	GCACAACCGGGATCTTTACTTC	138.4969
BC25843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84521	WGS84	BC25843	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25843_Pcan_Bot2011_IGS	S13-03	1	BC25843_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25843	Sample	BC25843	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-03	IGS	45	BC25843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-03	CGTAATCCTCAGTACTGGCTA	138.4969
BC25843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84521	WGS84	BC25843	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.336	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25843_Sspa_Oph2007_COI	S13-03	1	BC25843_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25843	Sample	BC25843	targeted	Present	Sample	Sspa_Oph2007_COI	S13-03	COI	45	BC25843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-03	GAAGGCCTTCCGATGATC	138.4969
BC25843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84521	WGS84	BC25843	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25843_Upin_Bot2011_COI	S13-03	1	BC25843_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25843	Sample	BC25843	targeted	Absent	Sample	Upin_Bot2011_COI	S13-03	COI	45	BC25843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.4969
BC25844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25844	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25844_Cjap_Sim2018_COI	S13-04	1	BC25844_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25844	Sample	BC25844	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-04	COI	45	BC25844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50784
BC25844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25844	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.49	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25844_Mgig_Bot2012_COI	S13-04	1	BC25844_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25844	Sample	BC25844	targeted	Present	Sample	Mgig_Bot2012_COI	S13-04	COI	45	BC25844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.50784
BC25845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83671	WGS84	BC25845	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25845_Cmae_Bot2011_COI	S13-05	1	BC25845_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25845	Sample	BC25845	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-05	COI	45	BC25845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-05	ATGAACAGTCTATCCTCCTTTAG	138.50903
BC25847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.7778	WGS84	BC25847	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25847_Aamu_Bax2006_COI	S13-07	1	BC25847_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25847	Sample	BC25847	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-07	COI	45	BC25847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-07	GCACAACCGGGATCTTTACTTC	138.51836
BC25847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.7778	WGS84	BC25847	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25847_Pcan_Bot2011_IGS	S13-07	1	BC25847_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25847	Sample	BC25847	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-07	IGS	45	BC25847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-07	CGTAATCCTCAGTACTGGCTA	138.51836
BC25847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.7778	WGS84	BC25847	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25847_Sspa_Oph2007_COI	S13-07	1	BC25847_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25847	Sample	BC25847	targeted	Absent	Sample	Sspa_Oph2007_COI	S13-07	COI	45	BC25847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-07	GAAGGCCTTCCGATGATC	138.51836
BC25847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.7778	WGS84	BC25847	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25847_Upin_Bot2011_COI	S13-07	1	BC25847_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25847	Sample	BC25847	targeted	Absent	Sample	Upin_Bot2011_COI	S13-07	COI	45	BC25847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.51836
BC25848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77898	WGS84	BC25848	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25848_Cjap_Sim2018_COI	S13-08	1	BC25848_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25848	Sample	BC25848	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-08	COI	45	BC25848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51781
BC25848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77898	WGS84	BC25848	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.747	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25848_Mgig_Bot2012_COI	S13-08	1	BC25848_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25848	Sample	BC25848	targeted	Present	Sample	Mgig_Bot2012_COI	S13-08	COI	45	BC25848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51781
BC25849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.78028	WGS84	BC25849	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25849_Cmae_Bot2011_COI	S13-09	1	BC25849_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25849	Sample	BC25849	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-09	COI	45	BC25849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-09	ATGAACAGTCTATCCTCCTTTAG	138.51745
BC25850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80929	WGS84	BC25850	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25850_Aamu_Bax2006_COI	S13-10	1	BC25850_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25850	Sample	BC25850	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-10	COI	45	BC25850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-10	GCACAACCGGGATCTTTACTTC	138.51861
BC25850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80929	WGS84	BC25850	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25850_Pcan_Bot2011_IGS	S13-10	1	BC25850_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25850	Sample	BC25850	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-10	IGS	45	BC25850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-10	CGTAATCCTCAGTACTGGCTA	138.51861
BC25850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80929	WGS84	BC25850	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.55	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25850_Sspa_Oph2007_COI	S13-10	1	BC25850_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25850	Sample	BC25850	targeted	Present	Sample	Sspa_Oph2007_COI	S13-10	COI	45	BC25850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-10	GAAGGCCTTCCGATGATC	138.51861
BC25850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80929	WGS84	BC25850	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25850_Upin_Bot2011_COI	S13-10	1	BC25850_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25850	Sample	BC25850	targeted	Absent	Sample	Upin_Bot2011_COI	S13-10	COI	45	BC25850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.51861
BC25851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80998	WGS84	BC25851	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25851_Cjap_Sim2018_COI	S13-11	1	BC25851_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25851	Sample	BC25851	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-11	COI	45	BC25851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51973
BC25851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80998	WGS84	BC25851	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.761	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25851_Mgig_Bot2012_COI	S13-11	1	BC25851_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25851	Sample	BC25851	targeted	Present	Sample	Mgig_Bot2012_COI	S13-11	COI	45	BC25851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51973
BC25852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81041	WGS84	BC25852	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25852_Cmae_Bot2011_COI	S13-12	1	BC25852_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25852	Sample	BC25852	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-12	COI	45	BC25852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-12	ATGAACAGTCTATCCTCCTTTAG	138.52065
BC25854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25854	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25854_Aamu_Bax2006_COI	S14-02	1	BC25854_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25854	Sample	BC25854	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-02	COI	45	BC25854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-02	GCACAACCGGGATCTTTACTTC	138.50776
BC25854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25854	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25854_Pcan_Bot2011_IGS	S14-02	1	BC25854_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25854	Sample	BC25854	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-02	IGS	45	BC25854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-02	CGTAATCCTCAGTACTGGCTA	138.50776
BC25854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25854	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25854_Sspa_Oph2007_COI	S14-02	1	BC25854_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25854	Sample	BC25854	targeted	Absent	Sample	Sspa_Oph2007_COI	S14-02	COI	45	BC25854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-02	GAAGGCCTTCCGATGATC	138.50776
BC25854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25854	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25854_Upin_Bot2011_COI	S14-02	1	BC25854_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25854	Sample	BC25854	targeted	Absent	Sample	Upin_Bot2011_COI	S14-02	COI	45	BC25854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.50776
BC25855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.8365	WGS84	BC25855	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25855_Cjap_Sim2018_COI	S14-03	1	BC25855_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25855	Sample	BC25855	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-03	COI	45	BC25855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50906
BC25855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.8365	WGS84	BC25855	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.663	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25855_Mgig_Bot2012_COI	S14-03	1	BC25855_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25855	Sample	BC25855	targeted	Present	Sample	Mgig_Bot2012_COI	S14-03	COI	45	BC25855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.50906
BC25856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83589	WGS84	BC25856	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25856_Cmae_Bot2011_COI	S14-04	1	BC25856_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25856	Sample	BC25856	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-04	COI	45	BC25856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-04	ATGAACAGTCTATCCTCCTTTAG	138.50814
BC25858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.809	WGS84	BC25858	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25858_Aamu_Bax2006_COI	S14-06	1	BC25858_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25858	Sample	BC25858	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-06	COI	45	BC25858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-06	GCACAACCGGGATCTTTACTTC	138.51736
BC25858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.809	WGS84	BC25858	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25858_Pcan_Bot2011_IGS	S14-06	1	BC25858_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25858	Sample	BC25858	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-06	IGS	45	BC25858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-06	CGTAATCCTCAGTACTGGCTA	138.51736
BC25858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.809	WGS84	BC25858	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.92	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25858_Sspa_Oph2007_COI	S14-06	1	BC25858_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25858	Sample	BC25858	targeted	Present	Sample	Sspa_Oph2007_COI	S14-06	COI	45	BC25858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-06	GAAGGCCTTCCGATGATC	138.51736
BC25858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.809	WGS84	BC25858	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25858_Upin_Bot2011_COI	S14-06	1	BC25858_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25858	Sample	BC25858	targeted	Absent	Sample	Upin_Bot2011_COI	S14-06	COI	45	BC25858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.51736
BC25859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80934	WGS84	BC25859	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25859_Cjap_Sim2018_COI	S14-07	1	BC25859_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25859	Sample	BC25859	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-07	COI	45	BC25859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51813
BC25859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80934	WGS84	BC25859	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.258	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25859_Mgig_Bot2012_COI	S14-07	1	BC25859_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25859	Sample	BC25859	targeted	Present	Sample	Mgig_Bot2012_COI	S14-07	COI	45	BC25859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51813
BC25861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81024	WGS84	BC25861	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25861_Aamu_Bax2006_COI	S14-09	1	BC25861_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25861	Sample	BC25861	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-09	COI	45	BC25861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-09	GCACAACCGGGATCTTTACTTC	138.52043
BC25861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81024	WGS84	BC25861	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25861_Pcan_Bot2011_IGS	S14-09	1	BC25861_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25861	Sample	BC25861	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-09	IGS	45	BC25861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-09	CGTAATCCTCAGTACTGGCTA	138.52043
BC25861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81024	WGS84	BC25861	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.94	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25861_Sspa_Oph2007_COI	S14-09	1	BC25861_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25861	Sample	BC25861	targeted	Present	Sample	Sspa_Oph2007_COI	S14-09	COI	45	BC25861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-09	GAAGGCCTTCCGATGATC	138.52043
BC25861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81024	WGS84	BC25861	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25861_Upin_Bot2011_COI	S14-09	1	BC25861_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25861	Sample	BC25861	targeted	Absent	Sample	Upin_Bot2011_COI	S14-09	COI	45	BC25861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.52043
BC25862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81126	WGS84	BC25862	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25862_Cjap_Sim2018_COI	S14-10	1	BC25862_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25862	Sample	BC25862	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-10	COI	45	BC25862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52292
BC25862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81126	WGS84	BC25862	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.142	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25862_Mgig_Bot2012_COI	S14-10	1	BC25862_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25862	Sample	BC25862	targeted	Present	Sample	Mgig_Bot2012_COI	S14-10	COI	45	BC25862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.52292
BC25863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77237	WGS84	BC25863	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25863_Cmae_Bot2011_COI	S15-01	1	BC25863_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25863	Sample	BC25863	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-01	COI	45	BC25863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-01	ATGAACAGTCTATCCTCCTTTAG	138.48606
BC25865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77213	WGS84	BC25865	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25865_Aamu_Bax2006_COI	S15-03	1	BC25865_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25865	Sample	BC25865	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-03	COI	45	BC25865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-03	GCACAACCGGGATCTTTACTTC	138.48451
BC25865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77213	WGS84	BC25865	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25865_Pcan_Bot2011_IGS	S15-03	1	BC25865_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25865	Sample	BC25865	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-03	IGS	45	BC25865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-03	CGTAATCCTCAGTACTGGCTA	138.48451
BC25865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77213	WGS84	BC25865	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25865_Sspa_Oph2007_COI	S15-03	1	BC25865_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25865	Sample	BC25865	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-03	COI	45	BC25865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-03	GAAGGCCTTCCGATGATC	138.48451
BC25865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77213	WGS84	BC25865	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25865_Upin_Bot2011_COI	S15-03	1	BC25865_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25865	Sample	BC25865	targeted	Absent	Sample	Upin_Bot2011_COI	S15-03	COI	45	BC25865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.48451
BC25866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77797	WGS84	BC25866	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25866_Cjap_Sim2018_COI	S15-04	1	BC25866_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25866	Sample	BC25866	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-04	COI	45	BC25866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5189
BC25866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77797	WGS84	BC25866	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.985	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25866_Mgig_Bot2012_COI	S15-04	1	BC25866_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25866	Sample	BC25866	targeted	Present	Sample	Mgig_Bot2012_COI	S15-04	COI	45	BC25866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.5189
BC25867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77886	WGS84	BC25867	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25867_Cmae_Bot2011_COI	S15-05	1	BC25867_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25867	Sample	BC25867	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-05	COI	45	BC25867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-05	ATGAACAGTCTATCCTCCTTTAG	138.51809
BC25869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80875	WGS84	BC25869	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25869_Aamu_Bax2006_COI	S15-07	1	BC25869_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25869	Sample	BC25869	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-07	COI	45	BC25869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-07	GCACAACCGGGATCTTTACTTC	138.51685
BC25869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80875	WGS84	BC25869	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25869_Pcan_Bot2011_IGS	S15-07	1	BC25869_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25869	Sample	BC25869	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-07	IGS	45	BC25869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-07	CGTAATCCTCAGTACTGGCTA	138.51685
BC25869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80875	WGS84	BC25869	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25869_Sspa_Oph2007_COI	S15-07	1	BC25869_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25869	Sample	BC25869	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-07	COI	45	BC25869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-07	GAAGGCCTTCCGATGATC	138.51685
BC25869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80875	WGS84	BC25869	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25869_Upin_Bot2011_COI	S15-07	1	BC25869_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25869	Sample	BC25869	targeted	Absent	Sample	Upin_Bot2011_COI	S15-07	COI	45	BC25869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.51685
BC25870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80904	WGS84	BC25870	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25870_Cmae_Bot2011_COI	S15-08	1	BC25870_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25870	Sample	BC25870	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-08	COI	45	BC25870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-08	ATGAACAGTCTATCCTCCTTTAG	138.51774
BC25872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83621	WGS84	BC25872	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25872_Aamu_Bax2006_COI	S15-10	1	BC25872_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25872	Sample	BC25872	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-10	COI	45	BC25872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-10	GCACAACCGGGATCTTTACTTC	138.50868
BC25872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83621	WGS84	BC25872	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25872_Pcan_Bot2011_IGS	S15-10	1	BC25872_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25872	Sample	BC25872	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-10	IGS	45	BC25872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-10	CGTAATCCTCAGTACTGGCTA	138.50868
BC25872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83621	WGS84	BC25872	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25872_Sspa_Oph2007_COI	S15-10	1	BC25872_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25872	Sample	BC25872	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-10	COI	45	BC25872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-10	GAAGGCCTTCCGATGATC	138.50868
BC25872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83621	WGS84	BC25872	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25872_Upin_Bot2011_COI	S15-10	1	BC25872_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25872	Sample	BC25872	targeted	Absent	Sample	Upin_Bot2011_COI	S15-10	COI	45	BC25872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.50868
BC25873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83683	WGS84	BC25873	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25873_Cjap_Sim2018_COI	S15-11	1	BC25873_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25873	Sample	BC25873	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-11	COI	45	BC25873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5099
BC25873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83683	WGS84	BC25873	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.119	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25873_Mgig_Bot2012_COI	S15-11	1	BC25873_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25873	Sample	BC25873	targeted	Present	Sample	Mgig_Bot2012_COI	S15-11	COI	45	BC25873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.5099
BC25874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83695	WGS84	BC25874	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25874_Cmae_Bot2011_COI	S15-12	1	BC25874_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25874	Sample	BC25874	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-12	COI	45	BC25874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-12	ATGAACAGTCTATCCTCCTTTAG	138.50914
BC25876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84566	WGS84	BC25876	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25876_Aamu_Bax2006_COI	S15-14	1	BC25876_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25876	Sample	BC25876	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-14	COI	45	BC25876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-14	GCACAACCGGGATCTTTACTTC	138.4971
BC25876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84566	WGS84	BC25876	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25876_Pcan_Bot2011_IGS	S15-14	1	BC25876_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25876	Sample	BC25876	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-14	IGS	45	BC25876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-14	CGTAATCCTCAGTACTGGCTA	138.4971
BC25876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84566	WGS84	BC25876	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25876_Sspa_Oph2007_COI	S15-14	1	BC25876_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25876	Sample	BC25876	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-14	COI	45	BC25876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-14	GAAGGCCTTCCGATGATC	138.4971
BC25876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84566	WGS84	BC25876	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25876_Upin_Bot2011_COI	S15-14	1	BC25876_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25876	Sample	BC25876	targeted	Absent	Sample	Upin_Bot2011_COI	S15-14	COI	45	BC25876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.4971
BC25877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84391	WGS84	BC25877	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25877_Cjap_Sim2018_COI	S15-15	1	BC25877_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25877	Sample	BC25877	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-15	COI	45	BC25877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49688
BC25877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84391	WGS84	BC25877	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.586	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25877_Mgig_Bot2012_COI	S15-15	1	BC25877_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25877	Sample	BC25877	targeted	Present	Sample	Mgig_Bot2012_COI	S15-15	COI	45	BC25877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.49688
BC25878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96022	WGS84	BC25878	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.701	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25878_Cmae_Bot2011_COI	T19-01	1	BC25878_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25878	Sample	BC25878	targeted	Present	Sample	Cmae_Bot2011_COI	T19-01	COI	45	BC25878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-01	ATGAACAGTCTATCCTCCTTTAG	147.52332
BC25880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.8101	WGS84	BC25880	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25880_Cjap_Sim2018_COI	T19-03	1	BC25880_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25880	Sample	BC25880	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-03	COI	45	BC25880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.50248
BC25880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.8101	WGS84	BC25880	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.38	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	17/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25880_Mgig_Bot2012_COI	T19-03	1	BC25880_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25880	Sample	BC25880	targeted	Present	Sample	Mgig_Bot2012_COI	T19-03	COI	45	BC25880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.50248
BC25881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96467	WGS84	BC25881	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25881_Cmae_Bot2011_COI	T19-04	1	BC25881_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25881	Sample	BC25881	targeted	Absent	Sample	Cmae_Bot2011_COI	T19-04	COI	45	BC25881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-04	ATGAACAGTCTATCCTCCTTTAG	147.51968
BC25883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96305	WGS84	BC25883	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.377	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25883_Aamu_Bax2006_COI	T19-06	1	BC25883_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25883	Sample	BC25883	targeted	Present	Sample	Aamu_Bax2006_COI	T19-06	COI	45	BC25883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-06	GCACAACCGGGATCTTTACTTC	147.53847
BC25883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96305	WGS84	BC25883	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25883_Pcan_Bot2011_IGS	T19-06	1	BC25883_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25883	Sample	BC25883	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-06	IGS	45	BC25883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-06	CGTAATCCTCAGTACTGGCTA	147.53847
BC25883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96305	WGS84	BC25883	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25883_Sspa_Oph2007_COI	T19-06	1	BC25883_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25883	Sample	BC25883	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-06	COI	45	BC25883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-06	GAAGGCCTTCCGATGATC	147.53847
BC25883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96305	WGS84	BC25883	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25883_Upin_Bot2011_COI	T19-06	1	BC25883_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25883	Sample	BC25883	targeted	Absent	Sample	Upin_Bot2011_COI	T19-06	COI	45	BC25883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.53847
BC25884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96014	WGS84	BC25884	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25884_Cjap_Sim2018_COI	T19-07	1	BC25884_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25884	Sample	BC25884	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-07	COI	45	BC25884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53014
BC25884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96014	WGS84	BC25884	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.116	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	18/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25884_Mgig_Bot2012_COI	T19-07	1	BC25884_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25884	Sample	BC25884	targeted	Present	Sample	Mgig_Bot2012_COI	T19-07	COI	45	BC25884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.53014
BC25885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95987	WGS84	BC25885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.364	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25885_Cmae_Bot2011_COI	T19-08	1	BC25885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25885	Sample	BC25885	targeted	Present	Sample	Cmae_Bot2011_COI	T19-08	COI	45	BC25885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-08	ATGAACAGTCTATCCTCCTTTAG	147.52361
BC25894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80318	WGS84	BC25894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25894_Aamu_Bax2006_COI	T19-17	1	BC25894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25894	Sample	BC25894	targeted	Absent	Sample	Aamu_Bax2006_COI	T19-17	COI	45	BC25894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-17	GCACAACCGGGATCTTTACTTC	147.46501
BC25894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80318	WGS84	BC25894	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25894_Pcan_Bot2011_IGS	T19-17	1	BC25894_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25894	Sample	BC25894	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-17	IGS	45	BC25894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-17	CGTAATCCTCAGTACTGGCTA	147.46501
BC25894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80318	WGS84	BC25894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25894_Sspa_Oph2007_COI	T19-17	1	BC25894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25894	Sample	BC25894	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-17	COI	45	BC25894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-17	GAAGGCCTTCCGATGATC	147.46501
BC25894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80318	WGS84	BC25894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25894_Upin_Bot2011_COI	T19-17	1	BC25894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25894	Sample	BC25894	targeted	Absent	Sample	Upin_Bot2011_COI	T19-17	COI	45	BC25894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-17	CTTTAATTACAGCGTTTTTATTGTTGT	147.46501
BC25895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25895	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25895_Cjap_Sim2018_COI	T19-18	1	BC25895_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25895	Sample	BC25895	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-18	COI	45	BC25895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.48557
BC25895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.531	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	17/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25895_Mgig_Bot2012_COI	T19-18	1	BC25895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25895	Sample	BC25895	targeted	Present	Sample	Mgig_Bot2012_COI	T19-18	COI	45	BC25895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-18	TCTTATTCGTTGGAGACTTTATAACCCT	147.48557
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29597_Aamu_Bax2006_COI	KEMB1	1	BC29597_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	GCACAACCGGGATCTTTACTTC	150.90962
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29597_Pcan_Bot2011_IGS	KEMB1	1	BC29597_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB1	IGS	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	CGTAATCCTCAGTACTGGCTA	150.90962
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29597_Sspa_Oph2007_COI	KEMB1	1	BC29597_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	GAAGGCCTTCCGATGATC	150.90962
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29597_Upin_Bot2011_COI	KEMB1	1	BC29597_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	CTTTAATTACAGCGTTTTTATTGTTGT	150.90962
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29597_Vgib_Wil2025_COI	KEMB1	1	BC29597_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	CCCCTCTTTACAAGTTCTTTAGGGT	150.90962
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29598_Asen_Wil2025_COI	KEMB2	1	BC29598_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	GTTGTTGTTACAACACATGCTCTTTTG	150.91231
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29598_Mgig_Bot2012_COI	KEMB2	1	BC29598_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	TCTTATTCGTTGGAGACTTTATAACCCT	150.91231
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29599_Cmae_Bot2011_COI	KEMB3	1	BC29599_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	ATGAACAGTCTATCCTCCTTTAG	150.9113
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29599_Msal_Bot2012_COI	KEMB3	1	BC29599_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	GYTAGTTCCRATGATGTTAGCTG	150.9113
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29601_Aamu_Bax2006_COI	KEMB5	1	BC29601_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	GCACAACCGGGATCTTTACTTC	150.91196
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29601_Pcan_Bot2011_IGS	KEMB5	1	BC29601_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB5	IGS	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	CGTAATCCTCAGTACTGGCTA	150.91196
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29601_Sspa_Oph2007_COI	KEMB5	1	BC29601_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	GAAGGCCTTCCGATGATC	150.91196
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29601_Upin_Bot2011_COI	KEMB5	1	BC29601_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	CTTTAATTACAGCGTTTTTATTGTTGT	150.91196
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29601_Vgib_Wil2025_COI	KEMB5	1	BC29601_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	CCCCTCTTTACAAGTTCTTTAGGGT	150.91196
BC29602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88101	WGS84	BC29602	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.41	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29602_Mgig_Bot2012_COI	NEWA46	1	BC29602_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29602	Sample	BC29602	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA46	COI	45	BC29602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA46	TCTTATTCGTTGGAGACTTTATAACCCT	151.75063
BC29609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2534	WGS84	BC29609	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29609_Aamu_Bax2006_COI	HAYA79	1	BC29609_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29609	Sample	BC29609	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA79	COI	45	BC29609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA79	GCACAACCGGGATCTTTACTTC	149.30644
BC29609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2534	WGS84	BC29609	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29609_Pcan_Bot2011_IGS	HAYA79	1	BC29609_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29609	Sample	BC29609	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA79	IGS	45	BC29609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA79	CGTAATCCTCAGTACTGGCTA	149.30644
BC29609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2534	WGS84	BC29609	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29609_Sspa_Oph2007_COI	HAYA79	1	BC29609_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29609	Sample	BC29609	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA79	COI	45	BC29609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA79	GAAGGCCTTCCGATGATC	149.30644
BC29609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2534	WGS84	BC29609	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29609_Upin_Bot2011_COI	HAYA79	1	BC29609_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29609	Sample	BC29609	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA79	COI	45	BC29609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA79	CTTTAATTACAGCGTTTTTATTGTTGT	149.30644
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2537	WGS84	BC29610	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29610_Cmae_Bot2011_COI	HAYA78	1	BC29610_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	ATGAACAGTCTATCCTCCTTTAG	149.30538
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2537	WGS84	BC29610	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29610_Msal_Bot2012_COI	HAYA78	1	BC29610_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	GYTAGTTCCRATGATGTTAGCTG	149.30538
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25463	WGS84	BC29612	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29612_Aamu_Bax2006_COI	HAYA76	1	BC29612_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	GCACAACCGGGATCTTTACTTC	149.3096
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25463	WGS84	BC29612	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29612_Pcan_Bot2011_IGS	HAYA76	1	BC29612_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA76	IGS	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	CGTAATCCTCAGTACTGGCTA	149.3096
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25463	WGS84	BC29612	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29612_Sspa_Oph2007_COI	HAYA76	1	BC29612_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	GAAGGCCTTCCGATGATC	149.3096
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25463	WGS84	BC29612	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29612_Upin_Bot2011_COI	HAYA76	1	BC29612_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	CTTTAATTACAGCGTTTTTATTGTTGT	149.3096
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25463	WGS84	BC29612	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29612_Vgib_Wil2025_COI	HAYA76	1	BC29612_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	CCCCTCTTTACAAGTTCTTTAGGGT	149.3096
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25406	WGS84	BC29613	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29613_Asen_Wil2025_COI	HAYA75	1	BC29613_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	GTTGTTGTTACAACACATGCTCTTTTG	149.30913
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25406	WGS84	BC29613	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29613_Mgig_Bot2012_COI	HAYA75	1	BC29613_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	TCTTATTCGTTGGAGACTTTATAACCCT	149.30913
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25515	WGS84	BC29614	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29614_Cmae_Bot2011_COI	HAYA74	1	BC29614_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	ATGAACAGTCTATCCTCCTTTAG	149.30727
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25515	WGS84	BC29614	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29614_Msal_Bot2012_COI	HAYA74	1	BC29614_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	GYTAGTTCCRATGATGTTAGCTG	149.30727
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25433	WGS84	BC29616	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29616_Aamu_Bax2006_COI	HAYA72	1	BC29616_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	GCACAACCGGGATCTTTACTTC	149.31174
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25433	WGS84	BC29616	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29616_Pcan_Bot2011_IGS	HAYA72	1	BC29616_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA72	IGS	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	CGTAATCCTCAGTACTGGCTA	149.31174
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25433	WGS84	BC29616	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29616_Sspa_Oph2007_COI	HAYA72	1	BC29616_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	GAAGGCCTTCCGATGATC	149.31174
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25433	WGS84	BC29616	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29616_Upin_Bot2011_COI	HAYA72	1	BC29616_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	CTTTAATTACAGCGTTTTTATTGTTGT	149.31174
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25433	WGS84	BC29616	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29616_Vgib_Wil2025_COI	HAYA72	1	BC29616_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	CCCCTCTTTACAAGTTCTTTAGGGT	149.31174
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25514	WGS84	BC29617	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29617_Asen_Wil2025_COI	HAYA71	1	BC29617_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	GTTGTTGTTACAACACATGCTCTTTTG	149.30984
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25514	WGS84	BC29617	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29617_Mgig_Bot2012_COI	HAYA71	1	BC29617_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	TCTTATTCGTTGGAGACTTTATAACCCT	149.30984
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25551	WGS84	BC29618	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29618_Cmae_Bot2011_COI	HAYA70	1	BC29618_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	ATGAACAGTCTATCCTCCTTTAG	149.30925
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25551	WGS84	BC29618	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29618_Msal_Bot2012_COI	HAYA70	1	BC29618_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	GYTAGTTCCRATGATGTTAGCTG	149.30925
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25554	WGS84	BC29620	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29620_Asen_Wil2025_COI	HAYA68	1	BC29620_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	GTTGTTGTTACAACACATGCTCTTTTG	149.31215
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25554	WGS84	BC29620	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29620_Mgig_Bot2012_COI	HAYA68	1	BC29620_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	TCTTATTCGTTGGAGACTTTATAACCCT	149.31215
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25542	WGS84	BC29621	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29621_Cmae_Bot2011_COI	HAYA67	1	BC29621_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	ATGAACAGTCTATCCTCCTTTAG	149.31127
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25542	WGS84	BC29621	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29621_Msal_Bot2012_COI	HAYA67	1	BC29621_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	GYTAGTTCCRATGATGTTAGCTG	149.31127
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25552	WGS84	BC29623	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29623_Aamu_Bax2006_COI	HAYA65	1	BC29623_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	GCACAACCGGGATCTTTACTTC	149.30799
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25552	WGS84	BC29623	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29623_Pcan_Bot2011_IGS	HAYA65	1	BC29623_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA65	IGS	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	CGTAATCCTCAGTACTGGCTA	149.30799
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25552	WGS84	BC29623	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29623_Sspa_Oph2007_COI	HAYA65	1	BC29623_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	GAAGGCCTTCCGATGATC	149.30799
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25552	WGS84	BC29623	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29623_Upin_Bot2011_COI	HAYA65	1	BC29623_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	CTTTAATTACAGCGTTTTTATTGTTGT	149.30799
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25552	WGS84	BC29623	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29623_Vgib_Wil2025_COI	HAYA65	1	BC29623_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	CCCCTCTTTACAAGTTCTTTAGGGT	149.30799
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25631	WGS84	BC29624	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29624_Asen_Wil2025_COI	HAYA64	1	BC29624_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	GTTGTTGTTACAACACATGCTCTTTTG	149.30702
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25631	WGS84	BC29624	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29624_Mgig_Bot2012_COI	HAYA64	1	BC29624_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	TCTTATTCGTTGGAGACTTTATAACCCT	149.30702
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25587	WGS84	BC29625	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29625_Cmae_Bot2011_COI	HAYA63	1	BC29625_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	ATGAACAGTCTATCCTCCTTTAG	149.31251
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25587	WGS84	BC29625	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29625_Msal_Bot2012_COI	HAYA63	1	BC29625_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	GYTAGTTCCRATGATGTTAGCTG	149.31251
BC29627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25593	WGS84	BC29627	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29627_Aamu_Bax2006_COI	HAYA61	1	BC29627_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29627	Sample	BC29627	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA61	COI	45	BC29627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA61	GCACAACCGGGATCTTTACTTC	149.31054
BC29627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25593	WGS84	BC29627	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29627_Pcan_Bot2011_IGS	HAYA61	1	BC29627_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29627	Sample	BC29627	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA61	IGS	45	BC29627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA61	CGTAATCCTCAGTACTGGCTA	149.31054
BC29627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25593	WGS84	BC29627	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29627_Sspa_Oph2007_COI	HAYA61	1	BC29627_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29627	Sample	BC29627	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA61	COI	45	BC29627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA61	GAAGGCCTTCCGATGATC	149.31054
BC29627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25593	WGS84	BC29627	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29627_Upin_Bot2011_COI	HAYA61	1	BC29627_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29627	Sample	BC29627	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA61	COI	45	BC29627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA61	CTTTAATTACAGCGTTTTTATTGTTGT	149.31054
BC29628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25702	WGS84	BC29628	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29628_Mgig_Bot2012_COI	HAYA60	1	BC29628_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29628	Sample	BC29628	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA60	COI	45	BC29628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA60	TCTTATTCGTTGGAGACTTTATAACCCT	149.30984
BC29629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25645	WGS84	BC29629	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29629_Cmae_Bot2011_COI	HAYA59	1	BC29629_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29629	Sample	BC29629	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA59	COI	45	BC29629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA59	ATGAACAGTCTATCCTCCTTTAG	149.30918
BC29629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25645	WGS84	BC29629	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29629_Msal_Bot2012_COI	HAYA59	1	BC29629_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29629	Sample	BC29629	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA59	COI	45	BC29629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA59	GYTAGTTCCRATGATGTTAGCTG	149.30918
BC29630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2565	WGS84	BC29630	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29630_Aamu_Bax2006_COI	HAYA58	1	BC29630_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29630	Sample	BC29630	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA58	COI	45	BC29630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA58	GCACAACCGGGATCTTTACTTC	149.30741
BC29630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2565	WGS84	BC29630	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29630_Pcan_Bot2011_IGS	HAYA58	1	BC29630_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29630	Sample	BC29630	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA58	IGS	45	BC29630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA58	CGTAATCCTCAGTACTGGCTA	149.30741
BC29630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2565	WGS84	BC29630	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29630_Sspa_Oph2007_COI	HAYA58	1	BC29630_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29630	Sample	BC29630	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA58	COI	45	BC29630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA58	GAAGGCCTTCCGATGATC	149.30741
BC29630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2565	WGS84	BC29630	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29630_Upin_Bot2011_COI	HAYA58	1	BC29630_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29630	Sample	BC29630	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA58	COI	45	BC29630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA58	CTTTAATTACAGCGTTTTTATTGTTGT	149.30741
BC29631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25743	WGS84	BC29631	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29631_Mgig_Bot2012_COI	HAYA57	1	BC29631_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29631	Sample	BC29631	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA57	COI	45	BC29631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA57	TCTTATTCGTTGGAGACTTTATAACCCT	149.3123
BC29632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25737	WGS84	BC29632	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29632_Cmae_Bot2011_COI	HAYA56	1	BC29632_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29632	Sample	BC29632	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA56	COI	45	BC29632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA56	ATGAACAGTCTATCCTCCTTTAG	149.31061
BC29632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25737	WGS84	BC29632	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29632_Msal_Bot2012_COI	HAYA56	1	BC29632_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29632	Sample	BC29632	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA56	COI	45	BC29632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA56	GYTAGTTCCRATGATGTTAGCTG	149.31061
BC29634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25781	WGS84	BC29634	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29634_Aamu_Bax2006_COI	HAYA54	1	BC29634_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29634	Sample	BC29634	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA54	COI	45	BC29634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA54	GCACAACCGGGATCTTTACTTC	149.30938
BC29634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25781	WGS84	BC29634	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29634_Pcan_Bot2011_IGS	HAYA54	1	BC29634_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29634	Sample	BC29634	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA54	IGS	45	BC29634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA54	CGTAATCCTCAGTACTGGCTA	149.30938
BC29634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25781	WGS84	BC29634	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29634_Sspa_Oph2007_COI	HAYA54	1	BC29634_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29634	Sample	BC29634	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA54	COI	45	BC29634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA54	GAAGGCCTTCCGATGATC	149.30938
BC29634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25781	WGS84	BC29634	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29634_Upin_Bot2011_COI	HAYA54	1	BC29634_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29634	Sample	BC29634	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA54	COI	45	BC29634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA54	CTTTAATTACAGCGTTTTTATTGTTGT	149.30938
BC29635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25792	WGS84	BC29635	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29635_Mgig_Bot2012_COI	HAYA53	1	BC29635_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29635	Sample	BC29635	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA53	COI	45	BC29635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA53	TCTTATTCGTTGGAGACTTTATAACCCT	149.30823
BC29636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25735	WGS84	BC29636	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29636_Cmae_Bot2011_COI	HAYA52	1	BC29636_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29636	Sample	BC29636	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA52	COI	45	BC29636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA52	ATGAACAGTCTATCCTCCTTTAG	149.31285
BC29636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25735	WGS84	BC29636	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29636_Msal_Bot2012_COI	HAYA52	1	BC29636_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29636	Sample	BC29636	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA52	COI	45	BC29636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA52	GYTAGTTCCRATGATGTTAGCTG	149.31285
BC29638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25789	WGS84	BC29638	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29638_Aamu_Bax2006_COI	HAYA50	1	BC29638_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29638	Sample	BC29638	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA50	COI	45	BC29638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA50	GCACAACCGGGATCTTTACTTC	149.30852
BC29638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25789	WGS84	BC29638	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29638_Pcan_Bot2011_IGS	HAYA50	1	BC29638_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29638	Sample	BC29638	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA50	IGS	45	BC29638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA50	CGTAATCCTCAGTACTGGCTA	149.30852
BC29638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25789	WGS84	BC29638	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29638_Sspa_Oph2007_COI	HAYA50	1	BC29638_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29638	Sample	BC29638	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA50	COI	45	BC29638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA50	GAAGGCCTTCCGATGATC	149.30852
BC29638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25789	WGS84	BC29638	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29638_Upin_Bot2011_COI	HAYA50	1	BC29638_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29638	Sample	BC29638	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA50	COI	45	BC29638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA50	CTTTAATTACAGCGTTTTTATTGTTGT	149.30852
BC29639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25811	WGS84	BC29639	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29639_Mgig_Bot2012_COI	HAYA49	1	BC29639_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29639	Sample	BC29639	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA49	COI	45	BC29639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA49	TCTTATTCGTTGGAGACTTTATAACCCT	149.31273
BC29641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25844	WGS84	BC29641	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29641_Aamu_Bax2006_COI	HAYA47	1	BC29641_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29641	Sample	BC29641	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA47	COI	45	BC29641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA47	GCACAACCGGGATCTTTACTTC	149.30923
BC29641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25844	WGS84	BC29641	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29641_Pcan_Bot2011_IGS	HAYA47	1	BC29641_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29641	Sample	BC29641	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA47	IGS	45	BC29641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA47	CGTAATCCTCAGTACTGGCTA	149.30923
BC29641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25844	WGS84	BC29641	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29641_Sspa_Oph2007_COI	HAYA47	1	BC29641_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29641	Sample	BC29641	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA47	COI	45	BC29641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA47	GAAGGCCTTCCGATGATC	149.30923
BC29641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25844	WGS84	BC29641	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29641_Upin_Bot2011_COI	HAYA47	1	BC29641_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29641	Sample	BC29641	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA47	COI	45	BC29641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA47	CTTTAATTACAGCGTTTTTATTGTTGT	149.30923
BC29642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25917	WGS84	BC29642	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29642_Mgig_Bot2012_COI	HAYA46	1	BC29642_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29642	Sample	BC29642	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA46	COI	45	BC29642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA46	TCTTATTCGTTGGAGACTTTATAACCCT	149.31302
BC29643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25875	WGS84	BC29643	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29643_Cmae_Bot2011_COI	HAYA45	1	BC29643_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29643	Sample	BC29643	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA45	COI	45	BC29643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA45	ATGAACAGTCTATCCTCCTTTAG	149.31316
BC29643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25875	WGS84	BC29643	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29643_Msal_Bot2012_COI	HAYA45	1	BC29643_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29643	Sample	BC29643	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA45	COI	45	BC29643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA45	GYTAGTTCCRATGATGTTAGCTG	149.31316
BC29645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25954	WGS84	BC29645	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29645_Aamu_Bax2006_COI	HAYA43	1	BC29645_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29645	Sample	BC29645	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA43	COI	45	BC29645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA43	GCACAACCGGGATCTTTACTTC	149.30874
BC29645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25954	WGS84	BC29645	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29645_Pcan_Bot2011_IGS	HAYA43	1	BC29645_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29645	Sample	BC29645	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA43	IGS	45	BC29645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA43	CGTAATCCTCAGTACTGGCTA	149.30874
BC29645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25954	WGS84	BC29645	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29645_Sspa_Oph2007_COI	HAYA43	1	BC29645_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29645	Sample	BC29645	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA43	COI	45	BC29645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA43	GAAGGCCTTCCGATGATC	149.30874
BC29645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25954	WGS84	BC29645	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29645_Upin_Bot2011_COI	HAYA43	1	BC29645_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29645	Sample	BC29645	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA43	COI	45	BC29645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA43	CTTTAATTACAGCGTTTTTATTGTTGT	149.30874
BC29646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25938	WGS84	BC29646	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29646_Mgig_Bot2012_COI	HAYA42	1	BC29646_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29646	Sample	BC29646	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA42	COI	45	BC29646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA42	TCTTATTCGTTGGAGACTTTATAACCCT	149.3134
BC29647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26013	WGS84	BC29647	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29647_Cmae_Bot2011_COI	HAYA41	1	BC29647_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29647	Sample	BC29647	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA41	COI	45	BC29647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA41	ATGAACAGTCTATCCTCCTTTAG	149.31281
BC29647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26013	WGS84	BC29647	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29647_Msal_Bot2012_COI	HAYA41	1	BC29647_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29647	Sample	BC29647	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA41	COI	45	BC29647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA41	GYTAGTTCCRATGATGTTAGCTG	149.31281
BC29649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26015	WGS84	BC29649	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29649_Aamu_Bax2006_COI	HAYA39	1	BC29649_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29649	Sample	BC29649	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA39	COI	45	BC29649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA39	GCACAACCGGGATCTTTACTTC	149.30916
BC29649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26015	WGS84	BC29649	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29649_Pcan_Bot2011_IGS	HAYA39	1	BC29649_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29649	Sample	BC29649	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA39	IGS	45	BC29649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA39	CGTAATCCTCAGTACTGGCTA	149.30916
BC29649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26015	WGS84	BC29649	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29649_Sspa_Oph2007_COI	HAYA39	1	BC29649_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29649	Sample	BC29649	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA39	COI	45	BC29649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA39	GAAGGCCTTCCGATGATC	149.30916
BC29649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26015	WGS84	BC29649	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29649_Upin_Bot2011_COI	HAYA39	1	BC29649_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29649	Sample	BC29649	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA39	COI	45	BC29649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA39	CTTTAATTACAGCGTTTTTATTGTTGT	149.30916
BC29650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26105	WGS84	BC29650	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29650_Cmae_Bot2011_COI	HAYA38	1	BC29650_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29650	Sample	BC29650	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA38	COI	45	BC29650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA38	ATGAACAGTCTATCCTCCTTTAG	149.31414
BC29650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26105	WGS84	BC29650	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29650_Msal_Bot2012_COI	HAYA38	1	BC29650_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29650	Sample	BC29650	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA38	COI	45	BC29650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA38	GYTAGTTCCRATGATGTTAGCTG	149.31414
BC29652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26079	WGS84	BC29652	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29652_Aamu_Bax2006_COI	HAYA36	1	BC29652_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29652	Sample	BC29652	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA36	COI	45	BC29652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA36	GCACAACCGGGATCTTTACTTC	149.31151
BC29652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26079	WGS84	BC29652	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29652_Pcan_Bot2011_IGS	HAYA36	1	BC29652_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29652	Sample	BC29652	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA36	IGS	45	BC29652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA36	CGTAATCCTCAGTACTGGCTA	149.31151
BC29652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26079	WGS84	BC29652	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29652_Sspa_Oph2007_COI	HAYA36	1	BC29652_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29652	Sample	BC29652	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA36	COI	45	BC29652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA36	GAAGGCCTTCCGATGATC	149.31151
BC29652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26079	WGS84	BC29652	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29652_Upin_Bot2011_COI	HAYA36	1	BC29652_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29652	Sample	BC29652	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA36	COI	45	BC29652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA36	CTTTAATTACAGCGTTTTTATTGTTGT	149.31151
BC29653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26043	WGS84	BC29653	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29653_Mgig_Bot2012_COI	HAYA35	1	BC29653_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29653	Sample	BC29653	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA35	COI	45	BC29653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA35	TCTTATTCGTTGGAGACTTTATAACCCT	149.30992
BC29654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26084	WGS84	BC29654	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29654_Cmae_Bot2011_COI	HAYA34	1	BC29654_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29654	Sample	BC29654	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA34	COI	45	BC29654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA34	ATGAACAGTCTATCCTCCTTTAG	149.31405
BC29654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26084	WGS84	BC29654	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29654_Msal_Bot2012_COI	HAYA34	1	BC29654_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29654	Sample	BC29654	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA34	COI	45	BC29654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA34	GYTAGTTCCRATGATGTTAGCTG	149.31405
BC29656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26191	WGS84	BC29656	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29656_Aamu_Bax2006_COI	HAYA32	1	BC29656_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29656	Sample	BC29656	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA32	COI	45	BC29656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA32	GCACAACCGGGATCTTTACTTC	149.31036
BC29656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26191	WGS84	BC29656	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29656_Pcan_Bot2011_IGS	HAYA32	1	BC29656_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29656	Sample	BC29656	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA32	IGS	45	BC29656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA32	CGTAATCCTCAGTACTGGCTA	149.31036
BC29656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26191	WGS84	BC29656	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29656_Sspa_Oph2007_COI	HAYA32	1	BC29656_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29656	Sample	BC29656	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA32	COI	45	BC29656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA32	GAAGGCCTTCCGATGATC	149.31036
BC29656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26191	WGS84	BC29656	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29656_Upin_Bot2011_COI	HAYA32	1	BC29656_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29656	Sample	BC29656	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA32	COI	45	BC29656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA32	CTTTAATTACAGCGTTTTTATTGTTGT	149.31036
BC29657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26186	WGS84	BC29657	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29657_Mgig_Bot2012_COI	HAYA31	1	BC29657_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29657	Sample	BC29657	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA31	COI	45	BC29657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA31	TCTTATTCGTTGGAGACTTTATAACCCT	149.3153
BC29658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26249	WGS84	BC29658	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29658_Cmae_Bot2011_COI	HAYA30	1	BC29658_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29658	Sample	BC29658	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA30	COI	45	BC29658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA30	ATGAACAGTCTATCCTCCTTTAG	149.31419
BC29658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26249	WGS84	BC29658	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29658_Msal_Bot2012_COI	HAYA30	1	BC29658_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29658	Sample	BC29658	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA30	COI	45	BC29658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA30	GYTAGTTCCRATGATGTTAGCTG	149.31419
BC29660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26306	WGS84	BC29660	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29660_Mgig_Bot2012_COI	HAYA28	1	BC29660_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29660	Sample	BC29660	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA28	COI	45	BC29660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA28	TCTTATTCGTTGGAGACTTTATAACCCT	149.31389
BC29661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26326	WGS84	BC29661	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29661_Cmae_Bot2011_COI	HAYA27	1	BC29661_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29661	Sample	BC29661	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA27	COI	45	BC29661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA27	ATGAACAGTCTATCCTCCTTTAG	149.31547
BC29661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26326	WGS84	BC29661	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29661_Msal_Bot2012_COI	HAYA27	1	BC29661_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29661	Sample	BC29661	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA27	COI	45	BC29661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA27	GYTAGTTCCRATGATGTTAGCTG	149.31547
BC29663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26424	WGS84	BC29663	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29663_Aamu_Bax2006_COI	HAYA25	1	BC29663_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29663	Sample	BC29663	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA25	COI	45	BC29663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA25	GCACAACCGGGATCTTTACTTC	149.3158
BC29663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26424	WGS84	BC29663	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29663_Pcan_Bot2011_IGS	HAYA25	1	BC29663_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29663	Sample	BC29663	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA25	IGS	45	BC29663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA25	CGTAATCCTCAGTACTGGCTA	149.3158
BC29663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26424	WGS84	BC29663	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29663_Sspa_Oph2007_COI	HAYA25	1	BC29663_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29663	Sample	BC29663	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA25	COI	45	BC29663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA25	GAAGGCCTTCCGATGATC	149.3158
BC29663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26424	WGS84	BC29663	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29663_Upin_Bot2011_COI	HAYA25	1	BC29663_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29663	Sample	BC29663	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA25	COI	45	BC29663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA25	CTTTAATTACAGCGTTTTTATTGTTGT	149.3158
BC29664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26401	WGS84	BC29664	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29664_Mgig_Bot2012_COI	HAYA24	1	BC29664_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29664	Sample	BC29664	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA24	COI	45	BC29664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA24	TCTTATTCGTTGGAGACTTTATAACCCT	149.31418
BC29665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26359	WGS84	BC29665	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29665_Cmae_Bot2011_COI	HAYA23	1	BC29665_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29665	Sample	BC29665	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA23	COI	45	BC29665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA23	ATGAACAGTCTATCCTCCTTTAG	149.31395
BC29665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26359	WGS84	BC29665	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29665_Msal_Bot2012_COI	HAYA23	1	BC29665_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29665	Sample	BC29665	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA23	COI	45	BC29665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA23	GYTAGTTCCRATGATGTTAGCTG	149.31395
BC29667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26507	WGS84	BC29667	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29667_Aamu_Bax2006_COI	HAYA21	1	BC29667_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29667	Sample	BC29667	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA21	COI	45	BC29667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA21	GCACAACCGGGATCTTTACTTC	149.31557
BC29667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26507	WGS84	BC29667	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29667_Pcan_Bot2011_IGS	HAYA21	1	BC29667_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29667	Sample	BC29667	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA21	IGS	45	BC29667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA21	CGTAATCCTCAGTACTGGCTA	149.31557
BC29667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26507	WGS84	BC29667	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29667_Sspa_Oph2007_COI	HAYA21	1	BC29667_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29667	Sample	BC29667	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA21	COI	45	BC29667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA21	GAAGGCCTTCCGATGATC	149.31557
BC29667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26507	WGS84	BC29667	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29667_Upin_Bot2011_COI	HAYA21	1	BC29667_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29667	Sample	BC29667	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA21	COI	45	BC29667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA21	CTTTAATTACAGCGTTTTTATTGTTGT	149.31557
BC29668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26436	WGS84	BC29668	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29668_Mgig_Bot2012_COI	HAYA20	1	BC29668_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29668	Sample	BC29668	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA20	COI	45	BC29668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA20	TCTTATTCGTTGGAGACTTTATAACCCT	149.31409
BC29669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26538	WGS84	BC29669	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29669_Cmae_Bot2011_COI	HAYA19	1	BC29669_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29669	Sample	BC29669	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA19	COI	45	BC29669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA19	ATGAACAGTCTATCCTCCTTTAG	149.3153
BC29669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26538	WGS84	BC29669	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29669_Msal_Bot2012_COI	HAYA19	1	BC29669_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29669	Sample	BC29669	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA19	COI	45	BC29669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA19	GYTAGTTCCRATGATGTTAGCTG	149.3153
BC29670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26505	WGS84	BC29670	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29670_Aamu_Bax2006_COI	HAYA18	1	BC29670_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29670	Sample	BC29670	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA18	COI	45	BC29670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA18	GCACAACCGGGATCTTTACTTC	149.31493
BC29670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26505	WGS84	BC29670	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29670_Pcan_Bot2011_IGS	HAYA18	1	BC29670_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29670	Sample	BC29670	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA18	IGS	45	BC29670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA18	CGTAATCCTCAGTACTGGCTA	149.31493
BC29670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26505	WGS84	BC29670	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29670_Sspa_Oph2007_COI	HAYA18	1	BC29670_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29670	Sample	BC29670	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA18	COI	45	BC29670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA18	GAAGGCCTTCCGATGATC	149.31493
BC29670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26505	WGS84	BC29670	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29670_Upin_Bot2011_COI	HAYA18	1	BC29670_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29670	Sample	BC29670	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA18	COI	45	BC29670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA18	CTTTAATTACAGCGTTTTTATTGTTGT	149.31493
BC29671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26574	WGS84	BC29671	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29671_Mgig_Bot2012_COI	HAYA17	1	BC29671_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29671	Sample	BC29671	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA17	COI	45	BC29671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA17	TCTTATTCGTTGGAGACTTTATAACCCT	149.31447
BC29672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26587	WGS84	BC29672	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29672_Cmae_Bot2011_COI	HAYA16	1	BC29672_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29672	Sample	BC29672	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA16	COI	45	BC29672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA16	ATGAACAGTCTATCCTCCTTTAG	149.3118
BC29672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26587	WGS84	BC29672	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29672_Msal_Bot2012_COI	HAYA16	1	BC29672_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29672	Sample	BC29672	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA16	COI	45	BC29672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA16	GYTAGTTCCRATGATGTTAGCTG	149.3118
BC29674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26576	WGS84	BC29674	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29674_Aamu_Bax2006_COI	HAYA14	1	BC29674_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29674	Sample	BC29674	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA14	COI	45	BC29674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA14	GCACAACCGGGATCTTTACTTC	149.31553
BC29674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26576	WGS84	BC29674	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29674_Pcan_Bot2011_IGS	HAYA14	1	BC29674_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29674	Sample	BC29674	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA14	IGS	45	BC29674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA14	CGTAATCCTCAGTACTGGCTA	149.31553
BC29674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26576	WGS84	BC29674	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29674_Sspa_Oph2007_COI	HAYA14	1	BC29674_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29674	Sample	BC29674	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA14	COI	45	BC29674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA14	GAAGGCCTTCCGATGATC	149.31553
BC29674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26576	WGS84	BC29674	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29674_Upin_Bot2011_COI	HAYA14	1	BC29674_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29674	Sample	BC29674	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA14	COI	45	BC29674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA14	CTTTAATTACAGCGTTTTTATTGTTGT	149.31553
BC29675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29675	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29675_Mgig_Bot2012_COI	HAYA13	1	BC29675_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29675	Sample	BC29675	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA13	COI	45	BC29675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA13	TCTTATTCGTTGGAGACTTTATAACCCT	149.31476
BC29676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29676	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29676_Cmae_Bot2011_COI	HAYA12	1	BC29676_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29676	Sample	BC29676	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA12	COI	45	BC29676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA12	ATGAACAGTCTATCCTCCTTTAG	149.31633
BC29676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29676	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29676_Msal_Bot2012_COI	HAYA12	1	BC29676_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29676	Sample	BC29676	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA12	COI	45	BC29676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA12	GYTAGTTCCRATGATGTTAGCTG	149.31633
BC29678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26727	WGS84	BC29678	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29678_Aamu_Bax2006_COI	HAYA10	1	BC29678_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29678	Sample	BC29678	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA10	COI	45	BC29678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA10	GCACAACCGGGATCTTTACTTC	149.31534
BC29678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26727	WGS84	BC29678	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29678_Pcan_Bot2011_IGS	HAYA10	1	BC29678_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29678	Sample	BC29678	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA10	IGS	45	BC29678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA10	CGTAATCCTCAGTACTGGCTA	149.31534
BC29678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26727	WGS84	BC29678	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29678_Sspa_Oph2007_COI	HAYA10	1	BC29678_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29678	Sample	BC29678	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA10	COI	45	BC29678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA10	GAAGGCCTTCCGATGATC	149.31534
BC29678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26727	WGS84	BC29678	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29678_Upin_Bot2011_COI	HAYA10	1	BC29678_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29678	Sample	BC29678	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA10	COI	45	BC29678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA10	CTTTAATTACAGCGTTTTTATTGTTGT	149.31534
BC29679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2668	WGS84	BC29679	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29679_Mgig_Bot2012_COI	HAYA9	1	BC29679_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29679	Sample	BC29679	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA9	COI	45	BC29679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA9	TCTTATTCGTTGGAGACTTTATAACCCT	149.31294
BC29681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26785	WGS84	BC29681	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29681_Aamu_Bax2006_COI	HAYA7	1	BC29681_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29681	Sample	BC29681	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA7	COI	45	BC29681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA7	GCACAACCGGGATCTTTACTTC	149.31684
BC29681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26785	WGS84	BC29681	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29681_Pcan_Bot2011_IGS	HAYA7	1	BC29681_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29681	Sample	BC29681	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA7	IGS	45	BC29681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA7	CGTAATCCTCAGTACTGGCTA	149.31684
BC29681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26785	WGS84	BC29681	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29681_Sspa_Oph2007_COI	HAYA7	1	BC29681_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29681	Sample	BC29681	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA7	COI	45	BC29681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA7	GAAGGCCTTCCGATGATC	149.31684
BC29681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26785	WGS84	BC29681	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29681_Upin_Bot2011_COI	HAYA7	1	BC29681_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29681	Sample	BC29681	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA7	COI	45	BC29681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA7	CTTTAATTACAGCGTTTTTATTGTTGT	149.31684
BC29682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26714	WGS84	BC29682	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29682_Mgig_Bot2012_COI	HAYA6	1	BC29682_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29682	Sample	BC29682	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA6	COI	45	BC29682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA6	TCTTATTCGTTGGAGACTTTATAACCCT	149.31552
BC29683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2681	WGS84	BC29683	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29683_Cmae_Bot2011_COI	HAYA5	1	BC29683_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29683	Sample	BC29683	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA5	COI	45	BC29683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA5	ATGAACAGTCTATCCTCCTTTAG	149.31408
BC29683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2681	WGS84	BC29683	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29683_Msal_Bot2012_COI	HAYA5	1	BC29683_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29683	Sample	BC29683	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA5	COI	45	BC29683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA5	GYTAGTTCCRATGATGTTAGCTG	149.31408
BC29685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26771	WGS84	BC29685	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29685_Aamu_Bax2006_COI	HAYA3	1	BC29685_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29685	Sample	BC29685	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA3	COI	45	BC29685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA3	GCACAACCGGGATCTTTACTTC	149.31608
BC29685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26771	WGS84	BC29685	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29685_Pcan_Bot2011_IGS	HAYA3	1	BC29685_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29685	Sample	BC29685	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA3	IGS	45	BC29685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA3	CGTAATCCTCAGTACTGGCTA	149.31608
BC29685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26771	WGS84	BC29685	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29685_Sspa_Oph2007_COI	HAYA3	1	BC29685_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29685	Sample	BC29685	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA3	COI	45	BC29685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA3	GAAGGCCTTCCGATGATC	149.31608
BC29685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26771	WGS84	BC29685	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29685_Upin_Bot2011_COI	HAYA3	1	BC29685_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29685	Sample	BC29685	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA3	COI	45	BC29685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA3	CTTTAATTACAGCGTTTTTATTGTTGT	149.31608
BC29686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26894	WGS84	BC29686	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29686_Mgig_Bot2012_COI	HAYA2	1	BC29686_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29686	Sample	BC29686	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA2	COI	45	BC29686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA2	TCTTATTCGTTGGAGACTTTATAACCCT	149.31393
BC29687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26989	WGS84	BC29687	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29687_Cmae_Bot2011_COI	HAYA1	1	BC29687_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29687	Sample	BC29687	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA1	COI	45	BC29687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA1	ATGAACAGTCTATCCTCCTTTAG	149.31431
BC29687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26989	WGS84	BC29687	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29687_Msal_Bot2012_COI	HAYA1	1	BC29687_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29687	Sample	BC29687	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA1	COI	45	BC29687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA1	GYTAGTTCCRATGATGTTAGCTG	149.31431
BC29689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18175	WGS84	BC29689	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29689_Aamu_Bax2006_COI	DEVA18	1	BC29689_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29689	Sample	BC29689	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA18	COI	45	BC29689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA18	GCACAACCGGGATCTTTACTTC	146.36453
BC29689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18175	WGS84	BC29689	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29689_Pcan_Bot2011_IGS	DEVA18	1	BC29689_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29689	Sample	BC29689	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA18	IGS	45	BC29689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA18	CGTAATCCTCAGTACTGGCTA	146.36453
BC29689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18175	WGS84	BC29689	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29689_Sspa_Oph2007_COI	DEVA18	1	BC29689_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29689	Sample	BC29689	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA18	COI	45	BC29689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA18	GAAGGCCTTCCGATGATC	146.36453
BC29689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18175	WGS84	BC29689	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29689_Upin_Bot2011_COI	DEVA18	1	BC29689_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29689	Sample	BC29689	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA18	COI	45	BC29689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA18	CTTTAATTACAGCGTTTTTATTGTTGT	146.36453
BC29690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18121	WGS84	BC29690	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29690_Cmae_Bot2011_COI	DEVA17	1	BC29690_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29690	Sample	BC29690	targeted	Absent	Sample	Cmae_Bot2011_COI	DEVA17	COI	45	BC29690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA17	ATGAACAGTCTATCCTCCTTTAG	146.36505
BC29690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18121	WGS84	BC29690	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29690_Msal_Bot2012_COI	DEVA17	1	BC29690_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29690	Sample	BC29690	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA17	COI	45	BC29690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA17	GYTAGTTCCRATGATGTTAGCTG	146.36505
BC29692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18176	WGS84	BC29692	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29692_Aamu_Bax2006_COI	DEVA16	1	BC29692_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29692	Sample	BC29692	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA16	COI	45	BC29692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA16	GCACAACCGGGATCTTTACTTC	146.36377
BC29692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18176	WGS84	BC29692	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29692_Pcan_Bot2011_IGS	DEVA16	1	BC29692_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29692	Sample	BC29692	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA16	IGS	45	BC29692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA16	CGTAATCCTCAGTACTGGCTA	146.36377
BC29692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18176	WGS84	BC29692	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29692_Sspa_Oph2007_COI	DEVA16	1	BC29692_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29692	Sample	BC29692	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA16	COI	45	BC29692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA16	GAAGGCCTTCCGATGATC	146.36377
BC29692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18176	WGS84	BC29692	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29692_Upin_Bot2011_COI	DEVA16	1	BC29692_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29692	Sample	BC29692	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA16	COI	45	BC29692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA16	CTTTAATTACAGCGTTTTTATTGTTGT	146.36377
BC29693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1824	WGS84	BC29693	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.193	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29693_Mgig_Bot2012_COI	DEVA15	1	BC29693_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29693	Sample	BC29693	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA15	COI	45	BC29693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA15	TCTTATTCGTTGGAGACTTTATAACCCT	146.36585
BC29694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18351	WGS84	BC29694	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29694_Cmae_Bot2011_COI	DEVA13	1	BC29694_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29694	Sample	BC29694	targeted	Absent	Sample	Cmae_Bot2011_COI	DEVA13	COI	45	BC29694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA13	ATGAACAGTCTATCCTCCTTTAG	146.36547
BC29694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18351	WGS84	BC29694	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29694_Msal_Bot2012_COI	DEVA13	1	BC29694_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29694	Sample	BC29694	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA13	COI	45	BC29694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA13	GYTAGTTCCRATGATGTTAGCTG	146.36547
BC29696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18545	WGS84	BC29696	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29696_Aamu_Bax2006_COI	DEVA10	1	BC29696_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29696	Sample	BC29696	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA10	COI	45	BC29696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA10	GCACAACCGGGATCTTTACTTC	146.36573
BC29696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18545	WGS84	BC29696	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29696_Pcan_Bot2011_IGS	DEVA10	1	BC29696_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29696	Sample	BC29696	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA10	IGS	45	BC29696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA10	CGTAATCCTCAGTACTGGCTA	146.36573
BC29696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18545	WGS84	BC29696	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29696_Sspa_Oph2007_COI	DEVA10	1	BC29696_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29696	Sample	BC29696	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA10	COI	45	BC29696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA10	GAAGGCCTTCCGATGATC	146.36573
BC29696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18545	WGS84	BC29696	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29696_Upin_Bot2011_COI	DEVA10	1	BC29696_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29696	Sample	BC29696	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA10	COI	45	BC29696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA10	CTTTAATTACAGCGTTTTTATTGTTGT	146.36573
BC29697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18569	WGS84	BC29697	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.037	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29697_Mgig_Bot2012_COI	DEVA9	1	BC29697_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29697	Sample	BC29697	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA9	COI	45	BC29697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA9	TCTTATTCGTTGGAGACTTTATAACCCT	146.36387
BC29698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18654	WGS84	BC29698	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.671	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29698_Cmae_Bot2011_COI	DEVA8	1	BC29698_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29698	Sample	BC29698	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA8	COI	45	BC29698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA8	ATGAACAGTCTATCCTCCTTTAG	146.36659
BC29698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18654	WGS84	BC29698	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29698_Msal_Bot2012_COI	DEVA8	1	BC29698_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29698	Sample	BC29698	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA8	COI	45	BC29698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA8	GYTAGTTCCRATGATGTTAGCTG	146.36659
BC29700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1878	WGS84	BC29700	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29700_Aamu_Bax2006_COI	DEVA5	1	BC29700_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29700	Sample	BC29700	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA5	COI	45	BC29700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA5	GCACAACCGGGATCTTTACTTC	146.3656
BC29700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1878	WGS84	BC29700	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29700_Pcan_Bot2011_IGS	DEVA5	1	BC29700_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29700	Sample	BC29700	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA5	IGS	45	BC29700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA5	CGTAATCCTCAGTACTGGCTA	146.3656
BC29700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1878	WGS84	BC29700	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29700_Sspa_Oph2007_COI	DEVA5	1	BC29700_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29700	Sample	BC29700	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA5	COI	45	BC29700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA5	GAAGGCCTTCCGATGATC	146.3656
BC29700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1878	WGS84	BC29700	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29700_Upin_Bot2011_COI	DEVA5	1	BC29700_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29700	Sample	BC29700	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA5	COI	45	BC29700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA5	CTTTAATTACAGCGTTTTTATTGTTGT	146.3656
BC29701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18884	WGS84	BC29701	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.122	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29701_Mgig_Bot2012_COI	DEVA4	1	BC29701_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29701	Sample	BC29701	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA4	COI	45	BC29701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA4	TCTTATTCGTTGGAGACTTTATAACCCT	146.36796
BC29702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18922	WGS84	BC29702	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.959	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29702_Cmae_Bot2011_COI	DEVA3	1	BC29702_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29702	Sample	BC29702	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA3	COI	45	BC29702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA3	ATGAACAGTCTATCCTCCTTTAG	146.3675
BC29702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18922	WGS84	BC29702	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29702_Msal_Bot2012_COI	DEVA3	1	BC29702_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29702	Sample	BC29702	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA3	COI	45	BC29702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA3	GYTAGTTCCRATGATGTTAGCTG	146.3675
BC29704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8817	WGS84	BC29704	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29704_Aamu_Bax2006_COI	NEWA45	1	BC29704_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29704	Sample	BC29704	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA45	COI	45	BC29704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA45	GCACAACCGGGATCTTTACTTC	151.7493
BC29704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8817	WGS84	BC29704	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29704_Pcan_Bot2011_IGS	NEWA45	1	BC29704_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29704	Sample	BC29704	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA45	IGS	45	BC29704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA45	CGTAATCCTCAGTACTGGCTA	151.7493
BC29704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8817	WGS84	BC29704	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29704_Sspa_Oph2007_COI	NEWA45	1	BC29704_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29704	Sample	BC29704	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA45	COI	45	BC29704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA45	GAAGGCCTTCCGATGATC	151.7493
BC29704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8817	WGS84	BC29704	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29704_Upin_Bot2011_COI	NEWA45	1	BC29704_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29704	Sample	BC29704	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA45	COI	45	BC29704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA45	CTTTAATTACAGCGTTTTTATTGTTGT	151.7493
BC29705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88231	WGS84	BC29705	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.82	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29705_Mgig_Bot2012_COI	NEWA44	1	BC29705_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29705	Sample	BC29705	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA44	COI	45	BC29705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA44	TCTTATTCGTTGGAGACTTTATAACCCT	151.75221
BC29706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88377	WGS84	BC29706	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29706_Cmae_Bot2011_COI	NEWA43	1	BC29706_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29706	Sample	BC29706	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA43	COI	45	BC29706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA43	ATGAACAGTCTATCCTCCTTTAG	151.75584
BC29706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88377	WGS84	BC29706	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29706_Msal_Bot2012_COI	NEWA43	1	BC29706_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29706	Sample	BC29706	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA43	COI	45	BC29706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA43	GYTAGTTCCRATGATGTTAGCTG	151.75584
BC29708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88557	WGS84	BC29708	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29708_Aamu_Bax2006_COI	NEWA41	1	BC29708_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29708	Sample	BC29708	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA41	COI	45	BC29708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA41	GCACAACCGGGATCTTTACTTC	151.75728
BC29708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88557	WGS84	BC29708	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29708_Pcan_Bot2011_IGS	NEWA41	1	BC29708_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29708	Sample	BC29708	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA41	IGS	45	BC29708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA41	CGTAATCCTCAGTACTGGCTA	151.75728
BC29708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88557	WGS84	BC29708	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29708_Sspa_Oph2007_COI	NEWA41	1	BC29708_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29708	Sample	BC29708	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA41	COI	45	BC29708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA41	GAAGGCCTTCCGATGATC	151.75728
BC29708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88557	WGS84	BC29708	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29708_Upin_Bot2011_COI	NEWA41	1	BC29708_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29708	Sample	BC29708	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA41	COI	45	BC29708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA41	CTTTAATTACAGCGTTTTTATTGTTGT	151.75728
BC29709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88524	WGS84	BC29709	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.997	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29709_Mgig_Bot2012_COI	NEWA40	1	BC29709_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29709	Sample	BC29709	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA40	COI	45	BC29709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA40	TCTTATTCGTTGGAGACTTTATAACCCT	151.7552
BC29711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88679	WGS84	BC29711	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29711_Aamu_Bax2006_COI	NEWA38	1	BC29711_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29711	Sample	BC29711	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA38	COI	45	BC29711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA38	GCACAACCGGGATCTTTACTTC	151.75868
BC29711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88679	WGS84	BC29711	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29711_Pcan_Bot2011_IGS	NEWA38	1	BC29711_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29711	Sample	BC29711	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA38	IGS	45	BC29711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA38	CGTAATCCTCAGTACTGGCTA	151.75868
BC29711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88679	WGS84	BC29711	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29711_Sspa_Oph2007_COI	NEWA38	1	BC29711_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29711	Sample	BC29711	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA38	COI	45	BC29711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA38	GAAGGCCTTCCGATGATC	151.75868
BC29711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88679	WGS84	BC29711	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29711_Upin_Bot2011_COI	NEWA38	1	BC29711_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29711	Sample	BC29711	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA38	COI	45	BC29711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA38	CTTTAATTACAGCGTTTTTATTGTTGT	151.75868
BC29712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88826	WGS84	BC29712	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.386	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29712_Mgig_Bot2012_COI	NEWA37	1	BC29712_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29712	Sample	BC29712	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA37	COI	45	BC29712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA37	TCTTATTCGTTGGAGACTTTATAACCCT	151.76569
BC29713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8887	WGS84	BC29713	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29713_Cmae_Bot2011_COI	NEWA36	1	BC29713_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29713	Sample	BC29713	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA36	COI	45	BC29713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA36	ATGAACAGTCTATCCTCCTTTAG	151.76318
BC29713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8887	WGS84	BC29713	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29713_Msal_Bot2012_COI	NEWA36	1	BC29713_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29713	Sample	BC29713	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA36	COI	45	BC29713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA36	GYTAGTTCCRATGATGTTAGCTG	151.76318
BC29715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88952	WGS84	BC29715	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29715_Aamu_Bax2006_COI	NEWA34	1	BC29715_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29715	Sample	BC29715	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA34	COI	45	BC29715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA34	GCACAACCGGGATCTTTACTTC	151.76945
BC29715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88952	WGS84	BC29715	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29715_Pcan_Bot2011_IGS	NEWA34	1	BC29715_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29715	Sample	BC29715	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA34	IGS	45	BC29715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA34	CGTAATCCTCAGTACTGGCTA	151.76945
BC29715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88952	WGS84	BC29715	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29715_Sspa_Oph2007_COI	NEWA34	1	BC29715_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29715	Sample	BC29715	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA34	COI	45	BC29715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA34	GAAGGCCTTCCGATGATC	151.76945
BC29715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88952	WGS84	BC29715	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29715_Upin_Bot2011_COI	NEWA34	1	BC29715_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29715	Sample	BC29715	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA34	COI	45	BC29715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA34	CTTTAATTACAGCGTTTTTATTGTTGT	151.76945
BC29716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88997	WGS84	BC29716	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.373	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29716_Mgig_Bot2012_COI	NEWA33	1	BC29716_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29716	Sample	BC29716	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA33	COI	45	BC29716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA33	TCTTATTCGTTGGAGACTTTATAACCCT	151.7669
BC29717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8901	WGS84	BC29717	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29717_Cmae_Bot2011_COI	NEWA32	1	BC29717_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29717	Sample	BC29717	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA32	COI	45	BC29717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA32	ATGAACAGTCTATCCTCCTTTAG	151.76482
BC29717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8901	WGS84	BC29717	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29717_Msal_Bot2012_COI	NEWA32	1	BC29717_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29717	Sample	BC29717	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA32	COI	45	BC29717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA32	GYTAGTTCCRATGATGTTAGCTG	151.76482
BC29719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89109	WGS84	BC29719	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29719_Aamu_Bax2006_COI	NEWA30	1	BC29719_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29719	Sample	BC29719	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA30	COI	45	BC29719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA30	GCACAACCGGGATCTTTACTTC	151.77088
BC29719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89109	WGS84	BC29719	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29719_Pcan_Bot2011_IGS	NEWA30	1	BC29719_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29719	Sample	BC29719	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA30	IGS	45	BC29719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA30	CGTAATCCTCAGTACTGGCTA	151.77088
BC29719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89109	WGS84	BC29719	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29719_Sspa_Oph2007_COI	NEWA30	1	BC29719_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29719	Sample	BC29719	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA30	COI	45	BC29719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA30	GAAGGCCTTCCGATGATC	151.77088
BC29719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89109	WGS84	BC29719	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29719_Upin_Bot2011_COI	NEWA30	1	BC29719_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29719	Sample	BC29719	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA30	COI	45	BC29719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA30	CTTTAATTACAGCGTTTTTATTGTTGT	151.77088
BC29720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89121	WGS84	BC29720	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29720_Cmae_Bot2011_COI	NEWA29	1	BC29720_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29720	Sample	BC29720	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA29	COI	45	BC29720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA29	ATGAACAGTCTATCCTCCTTTAG	151.76873
BC29720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89121	WGS84	BC29720	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29720_Msal_Bot2012_COI	NEWA29	1	BC29720_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29720	Sample	BC29720	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA29	COI	45	BC29720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA29	GYTAGTTCCRATGATGTTAGCTG	151.76873
BC29722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89277	WGS84	BC29722	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29722_Aamu_Bax2006_COI	NEWA27	1	BC29722_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29722	Sample	BC29722	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA27	COI	45	BC29722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA27	GCACAACCGGGATCTTTACTTC	151.77197
BC29722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89277	WGS84	BC29722	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29722_Pcan_Bot2011_IGS	NEWA27	1	BC29722_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29722	Sample	BC29722	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA27	IGS	45	BC29722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA27	CGTAATCCTCAGTACTGGCTA	151.77197
BC29722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89277	WGS84	BC29722	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29722_Sspa_Oph2007_COI	NEWA27	1	BC29722_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29722	Sample	BC29722	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA27	COI	45	BC29722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA27	GAAGGCCTTCCGATGATC	151.77197
BC29722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89277	WGS84	BC29722	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29722_Upin_Bot2011_COI	NEWA27	1	BC29722_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29722	Sample	BC29722	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA27	COI	45	BC29722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA27	CTTTAATTACAGCGTTTTTATTGTTGT	151.77197
BC29723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89279	WGS84	BC29723	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.483	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29723_Mgig_Bot2012_COI	NEWA26	1	BC29723_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29723	Sample	BC29723	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA26	COI	45	BC29723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA26	TCTTATTCGTTGGAGACTTTATAACCCT	151.77002
BC29724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89331	WGS84	BC29724	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29724_Cmae_Bot2011_COI	NEWA25	1	BC29724_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29724	Sample	BC29724	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA25	COI	45	BC29724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA25	ATGAACAGTCTATCCTCCTTTAG	151.76761
BC29724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89331	WGS84	BC29724	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29724_Msal_Bot2012_COI	NEWA25	1	BC29724_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29724	Sample	BC29724	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA25	COI	45	BC29724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA25	GYTAGTTCCRATGATGTTAGCTG	151.76761
BC29726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89485	WGS84	BC29726	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29726_Aamu_Bax2006_COI	NEWA23	1	BC29726_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29726	Sample	BC29726	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA23	COI	45	BC29726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA23	GCACAACCGGGATCTTTACTTC	151.76897
BC29726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89485	WGS84	BC29726	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29726_Pcan_Bot2011_IGS	NEWA23	1	BC29726_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29726	Sample	BC29726	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA23	IGS	45	BC29726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA23	CGTAATCCTCAGTACTGGCTA	151.76897
BC29726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89485	WGS84	BC29726	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29726_Sspa_Oph2007_COI	NEWA23	1	BC29726_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29726	Sample	BC29726	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA23	COI	45	BC29726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA23	GAAGGCCTTCCGATGATC	151.76897
BC29726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89485	WGS84	BC29726	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29726_Upin_Bot2011_COI	NEWA23	1	BC29726_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29726	Sample	BC29726	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA23	COI	45	BC29726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA23	CTTTAATTACAGCGTTTTTATTGTTGT	151.76897
BC29727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89627	WGS84	BC29727	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.58	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29727_Mgig_Bot2012_COI	NEWA22	1	BC29727_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29727	Sample	BC29727	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA22	COI	45	BC29727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA22	TCTTATTCGTTGGAGACTTTATAACCCT	151.77242
BC29728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89642	WGS84	BC29728	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29728_Cmae_Bot2011_COI	NEWA21	1	BC29728_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29728	Sample	BC29728	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA21	COI	45	BC29728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA21	ATGAACAGTCTATCCTCCTTTAG	151.7701
BC29728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89642	WGS84	BC29728	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29728_Msal_Bot2012_COI	NEWA21	1	BC29728_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29728	Sample	BC29728	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA21	COI	45	BC29728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA21	GYTAGTTCCRATGATGTTAGCTG	151.7701
BC29730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91424	WGS84	BC29730	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.364	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29730_Mgig_Bot2012_COI	NEWA19	1	BC29730_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29730	Sample	BC29730	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA19	COI	45	BC29730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA19	TCTTATTCGTTGGAGACTTTATAACCCT	151.77313
BC29731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91522	WGS84	BC29731	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29731_Cmae_Bot2011_COI	NEWA18	1	BC29731_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29731	Sample	BC29731	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA18	COI	45	BC29731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA18	ATGAACAGTCTATCCTCCTTTAG	151.77531
BC29731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91522	WGS84	BC29731	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29731_Msal_Bot2012_COI	NEWA18	1	BC29731_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29731	Sample	BC29731	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA18	COI	45	BC29731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA18	GYTAGTTCCRATGATGTTAGCTG	151.77531
BC29733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91741	WGS84	BC29733	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29733_Aamu_Bax2006_COI	NEWA16	1	BC29733_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29733	Sample	BC29733	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA16	COI	45	BC29733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA16	GCACAACCGGGATCTTTACTTC	151.774
BC29733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91741	WGS84	BC29733	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29733_Pcan_Bot2011_IGS	NEWA16	1	BC29733_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29733	Sample	BC29733	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA16	IGS	45	BC29733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA16	CGTAATCCTCAGTACTGGCTA	151.774
BC29733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91741	WGS84	BC29733	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29733_Sspa_Oph2007_COI	NEWA16	1	BC29733_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29733	Sample	BC29733	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA16	COI	45	BC29733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA16	GAAGGCCTTCCGATGATC	151.774
BC29733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91741	WGS84	BC29733	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29733_Upin_Bot2011_COI	NEWA16	1	BC29733_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29733	Sample	BC29733	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA16	COI	45	BC29733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA16	CTTTAATTACAGCGTTTTTATTGTTGT	151.774
BC29734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91799	WGS84	BC29734	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.245	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29734_Mgig_Bot2012_COI	NEWA15	1	BC29734_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29734	Sample	BC29734	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA15	COI	45	BC29734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA15	TCTTATTCGTTGGAGACTTTATAACCCT	151.77576
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29735_Cmae_Bot2011_COI	NEWA14	1	BC29735_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	ATGAACAGTCTATCCTCCTTTAG	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29735_Hsan_Wil2021_COI	NEWA14	1	BC29735_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	CCTGGGCCGGTATAGTAGGT	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29735_Mros_Gib2022_COI	NEWA14	1	BC29735_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29735_Msal_Bot2012_COI	NEWA14	1	BC29735_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	GYTAGTTCCRATGATGTTAGCTG	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29735_Pvir_Dia2013_COI	NEWA14	1	BC29735_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	CTTAGTGGCATTAATTCGDAATCC	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29735_Rhar_Sim2018_COI	NEWA14	1	BC29735_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	CTGCTGCTATTGCTCATG	151.777
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29737_Aamu_Bax2006_COI	NEWA12	1	BC29737_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	GCACAACCGGGATCTTTACTTC	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29737_Pcan_Bot2011_IGS	NEWA12	1	BC29737_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA12	IGS	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	CGTAATCCTCAGTACTGGCTA	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29737_Pper_Dia2013_COI	NEWA12	1	BC29737_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	CTTAGTGGCATTAATTCGDAATCC	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29737_Sspa_Oph2007_COI	NEWA12	1	BC29737_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	GAAGGCCTTCCGATGATC	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29737_Upin_Bot2011_COI	NEWA12	1	BC29737_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	CTTTAATTACAGCGTTTTTATTGTTGT	151.77158
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29738_Cjap_Sim2018_COI	NEWA11	1	BC29738_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29738_Esin_And2018_Cyb	NEWA11	1	BC29738_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA11	Cytb	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	ACCCCTCCTCATATCCAACCA	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.747	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29738_Mgig_Bot2012_COI	NEWA11	1	BC29738_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	TCTTATTCGTTGGAGACTTTATAACCCT	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29738_Mjap_Gib2022_COI	NEWA11	1	BC29738_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	AATTTAATTGTGACTAGGCATGGTCTG	151.77323
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29739_Cmae_Bot2011_COI	NEWA10	1	BC29739_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	ATGAACAGTCTATCCTCCTTTAG	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29739_Hsan_Wil2021_COI	NEWA10	1	BC29739_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	CCTGGGCCGGTATAGTAGGT	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29739_Mros_Gib2022_COI	NEWA10	1	BC29739_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29739_Msal_Bot2012_COI	NEWA10	1	BC29739_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	GYTAGTTCCRATGATGTTAGCTG	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29739_Pvir_Dia2013_COI	NEWA10	1	BC29739_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	CTTAGTGGCATTAATTCGDAATCC	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29739_Rhar_Sim2018_COI	NEWA10	1	BC29739_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	CTGCTGCTATTGCTCATG	151.77133
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29740_Aamu_Bax2006_COI	NEWA9	1	BC29740_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	GCACAACCGGGATCTTTACTTC	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29740_Pcan_Bot2011_IGS	NEWA9	1	BC29740_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA9	IGS	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	CGTAATCCTCAGTACTGGCTA	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29740_Pper_Dia2013_COI	NEWA9	1	BC29740_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	CTTAGTGGCATTAATTCGDAATCC	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29740_Sspa_Oph2007_COI	NEWA9	1	BC29740_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	GAAGGCCTTCCGATGATC	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29740_Upin_Bot2011_COI	NEWA9	1	BC29740_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	CTTTAATTACAGCGTTTTTATTGTTGT	151.7724
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29741_Cjap_Sim2018_COI	NEWA8	1	BC29741_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29741_Esin_And2018_Cyb	NEWA8	1	BC29741_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA8	Cytb	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	ACCCCTCCTCATATCCAACCA	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.671	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29741_Mgig_Bot2012_COI	NEWA8	1	BC29741_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	TCTTATTCGTTGGAGACTTTATAACCCT	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29741_Mjap_Gib2022_COI	NEWA8	1	BC29741_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	AATTTAATTGTGACTAGGCATGGTCTG	151.77154
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29742_Cmae_Bot2011_COI	NEWA7	1	BC29742_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	ATGAACAGTCTATCCTCCTTTAG	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29742_Hsan_Wil2021_COI	NEWA7	1	BC29742_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	CCTGGGCCGGTATAGTAGGT	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29742_Mros_Gib2022_COI	NEWA7	1	BC29742_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29742_Msal_Bot2012_COI	NEWA7	1	BC29742_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	GYTAGTTCCRATGATGTTAGCTG	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29742_Pvir_Dia2013_COI	NEWA7	1	BC29742_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	CTTAGTGGCATTAATTCGDAATCC	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29742_Rhar_Sim2018_COI	NEWA7	1	BC29742_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	CTGCTGCTATTGCTCATG	151.76974
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29744_Aamu_Bax2006_COI	NEWA5	1	BC29744_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	GCACAACCGGGATCTTTACTTC	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29744_Pcan_Bot2011_IGS	NEWA5	1	BC29744_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA5	IGS	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	CGTAATCCTCAGTACTGGCTA	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29744_Pper_Dia2013_COI	NEWA5	1	BC29744_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	CTTAGTGGCATTAATTCGDAATCC	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29744_Sspa_Oph2007_COI	NEWA5	1	BC29744_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	GAAGGCCTTCCGATGATC	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29744_Upin_Bot2011_COI	NEWA5	1	BC29744_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	CTTTAATTACAGCGTTTTTATTGTTGT	151.76814
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29745_Cjap_Sim2018_COI	NEWA4	1	BC29745_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29745_Esin_And2018_Cyb	NEWA4	1	BC29745_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA4	Cytb	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	ACCCCTCCTCATATCCAACCA	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.118	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29745_Mgig_Bot2012_COI	NEWA4	1	BC29745_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	TCTTATTCGTTGGAGACTTTATAACCCT	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29745_Mjap_Gib2022_COI	NEWA4	1	BC29745_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	AATTTAATTGTGACTAGGCATGGTCTG	151.77353
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29746_Cmae_Bot2011_COI	NEWA3	1	BC29746_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	ATGAACAGTCTATCCTCCTTTAG	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29746_Hsan_Wil2021_COI	NEWA3	1	BC29746_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	CCTGGGCCGGTATAGTAGGT	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29746_Mros_Gib2022_COI	NEWA3	1	BC29746_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29746_Msal_Bot2012_COI	NEWA3	1	BC29746_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	GYTAGTTCCRATGATGTTAGCTG	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29746_Pvir_Dia2013_COI	NEWA3	1	BC29746_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	CTTAGTGGCATTAATTCGDAATCC	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29746_Rhar_Sim2018_COI	NEWA3	1	BC29746_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	CTGCTGCTATTGCTCATG	151.77067
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29748_Aamu_Bax2006_COI	NEWA1	1	BC29748_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	GCACAACCGGGATCTTTACTTC	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29748_Pcan_Bot2011_IGS	NEWA1	1	BC29748_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA1	IGS	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	CGTAATCCTCAGTACTGGCTA	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29748_Pper_Dia2013_COI	NEWA1	1	BC29748_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	CTTAGTGGCATTAATTCGDAATCC	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29748_Sspa_Oph2007_COI	NEWA1	1	BC29748_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	GAAGGCCTTCCGATGATC	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29748_Upin_Bot2011_COI	NEWA1	1	BC29748_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	CTTTAATTACAGCGTTTTTATTGTTGT	151.76515
BC29749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4533	WGS84	BC29749	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.366	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29749_Mgig_Bot2012_COI	KEMA35	1	BC29749_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29749	Sample	BC29749	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA35	COI	45	BC29749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA35	TCTTATTCGTTGGAGACTTTATAACCCT	150.8953
BC29751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45615	WGS84	BC29751	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29751_Aamu_Bax2006_COI	KEMA33	1	BC29751_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29751	Sample	BC29751	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA33	COI	45	BC29751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA33	GCACAACCGGGATCTTTACTTC	150.88729
BC29751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45615	WGS84	BC29751	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29751_Pcan_Bot2011_IGS	KEMA33	1	BC29751_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29751	Sample	BC29751	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA33	IGS	45	BC29751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA33	CGTAATCCTCAGTACTGGCTA	150.88729
BC29751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45615	WGS84	BC29751	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29751_Sspa_Oph2007_COI	KEMA33	1	BC29751_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29751	Sample	BC29751	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA33	COI	45	BC29751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA33	GAAGGCCTTCCGATGATC	150.88729
BC29751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45615	WGS84	BC29751	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29751_Upin_Bot2011_COI	KEMA33	1	BC29751_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29751	Sample	BC29751	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA33	COI	45	BC29751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA33	CTTTAATTACAGCGTTTTTATTGTTGT	150.88729
BC29752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45566	WGS84	BC29752	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.602	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29752_Mgig_Bot2012_COI	KEMA32	1	BC29752_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29752	Sample	BC29752	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA32	COI	45	BC29752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA32	TCTTATTCGTTGGAGACTTTATAACCCT	150.89579
BC29753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45621	WGS84	BC29753	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29753_Cmae_Bot2011_COI	KEMA31	1	BC29753_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29753	Sample	BC29753	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA31	COI	45	BC29753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA31	ATGAACAGTCTATCCTCCTTTAG	150.89105
BC29753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45621	WGS84	BC29753	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29753_Msal_Bot2012_COI	KEMA31	1	BC29753_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29753	Sample	BC29753	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA31	COI	45	BC29753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA31	GYTAGTTCCRATGATGTTAGCTG	150.89105
BC29755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45745	WGS84	BC29755	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29755_Aamu_Bax2006_COI	KEMA29	1	BC29755_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29755	Sample	BC29755	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA29	COI	45	BC29755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA29	GCACAACCGGGATCTTTACTTC	150.89471
BC29755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45745	WGS84	BC29755	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29755_Pcan_Bot2011_IGS	KEMA29	1	BC29755_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29755	Sample	BC29755	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA29	IGS	45	BC29755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA29	CGTAATCCTCAGTACTGGCTA	150.89471
BC29755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45745	WGS84	BC29755	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29755_Sspa_Oph2007_COI	KEMA29	1	BC29755_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29755	Sample	BC29755	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA29	COI	45	BC29755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA29	GAAGGCCTTCCGATGATC	150.89471
BC29755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45745	WGS84	BC29755	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29755_Upin_Bot2011_COI	KEMA29	1	BC29755_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29755	Sample	BC29755	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA29	COI	45	BC29755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA29	CTTTAATTACAGCGTTTTTATTGTTGT	150.89471
BC29756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45774	WGS84	BC29756	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.901	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29756_Mgig_Bot2012_COI	KEMA28	1	BC29756_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29756	Sample	BC29756	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA28	COI	45	BC29756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA28	TCTTATTCGTTGGAGACTTTATAACCCT	150.89314
BC29757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45868	WGS84	BC29757	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29757_Cmae_Bot2011_COI	KEMA27	1	BC29757_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29757	Sample	BC29757	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA27	COI	45	BC29757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA27	ATGAACAGTCTATCCTCCTTTAG	150.89076
BC29757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45868	WGS84	BC29757	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29757_Msal_Bot2012_COI	KEMA27	1	BC29757_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29757	Sample	BC29757	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA27	COI	45	BC29757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA27	GYTAGTTCCRATGATGTTAGCTG	150.89076
BC29759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45991	WGS84	BC29759	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29759_Aamu_Bax2006_COI	KEMA25	1	BC29759_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29759	Sample	BC29759	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA25	COI	45	BC29759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA25	GCACAACCGGGATCTTTACTTC	150.89441
BC29759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45991	WGS84	BC29759	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29759_Pcan_Bot2011_IGS	KEMA25	1	BC29759_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29759	Sample	BC29759	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA25	IGS	45	BC29759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA25	CGTAATCCTCAGTACTGGCTA	150.89441
BC29759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45991	WGS84	BC29759	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29759_Sspa_Oph2007_COI	KEMA25	1	BC29759_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29759	Sample	BC29759	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA25	COI	45	BC29759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA25	GAAGGCCTTCCGATGATC	150.89441
BC29759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45991	WGS84	BC29759	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29759_Upin_Bot2011_COI	KEMA25	1	BC29759_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29759	Sample	BC29759	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA25	COI	45	BC29759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA25	CTTTAATTACAGCGTTTTTATTGTTGT	150.89441
BC29760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45994	WGS84	BC29760	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29760_Cmae_Bot2011_COI	KEMA24	1	BC29760_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29760	Sample	BC29760	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA24	COI	45	BC29760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA24	ATGAACAGTCTATCCTCCTTTAG	150.8924
BC29760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45994	WGS84	BC29760	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29760_Msal_Bot2012_COI	KEMA24	1	BC29760_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29760	Sample	BC29760	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA24	COI	45	BC29760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA24	GYTAGTTCCRATGATGTTAGCTG	150.8924
BC29762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46148	WGS84	BC29762	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29762_Aamu_Bax2006_COI	KEMA22	1	BC29762_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29762	Sample	BC29762	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA22	COI	45	BC29762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA22	GCACAACCGGGATCTTTACTTC	150.89598
BC29762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46148	WGS84	BC29762	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29762_Pcan_Bot2011_IGS	KEMA22	1	BC29762_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29762	Sample	BC29762	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA22	IGS	45	BC29762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA22	CGTAATCCTCAGTACTGGCTA	150.89598
BC29762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46148	WGS84	BC29762	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29762_Sspa_Oph2007_COI	KEMA22	1	BC29762_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29762	Sample	BC29762	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA22	COI	45	BC29762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA22	GAAGGCCTTCCGATGATC	150.89598
BC29762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46148	WGS84	BC29762	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29762_Upin_Bot2011_COI	KEMA22	1	BC29762_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29762	Sample	BC29762	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA22	COI	45	BC29762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA22	CTTTAATTACAGCGTTTTTATTGTTGT	150.89598
BC29763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46143	WGS84	BC29763	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.047	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29763_Mgig_Bot2012_COI	KEMA21	1	BC29763_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29763	Sample	BC29763	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA21	COI	45	BC29763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA21	TCTTATTCGTTGGAGACTTTATAACCCT	150.89383
BC29764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46245	WGS84	BC29764	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29764_Cmae_Bot2011_COI	KEMA20	1	BC29764_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29764	Sample	BC29764	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA20	COI	45	BC29764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA20	ATGAACAGTCTATCCTCCTTTAG	150.89685
BC29764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46245	WGS84	BC29764	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29764_Msal_Bot2012_COI	KEMA20	1	BC29764_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29764	Sample	BC29764	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA20	COI	45	BC29764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA20	GYTAGTTCCRATGATGTTAGCTG	150.89685
BC29766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46396	WGS84	BC29766	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29766_Aamu_Bax2006_COI	KEMA18	1	BC29766_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29766	Sample	BC29766	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA18	COI	45	BC29766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA18	GCACAACCGGGATCTTTACTTC	150.90323
BC29766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46396	WGS84	BC29766	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29766_Pcan_Bot2011_IGS	KEMA18	1	BC29766_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29766	Sample	BC29766	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA18	IGS	45	BC29766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA18	CGTAATCCTCAGTACTGGCTA	150.90323
BC29766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46396	WGS84	BC29766	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29766_Sspa_Oph2007_COI	KEMA18	1	BC29766_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29766	Sample	BC29766	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA18	COI	45	BC29766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA18	GAAGGCCTTCCGATGATC	150.90323
BC29766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46396	WGS84	BC29766	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29766_Upin_Bot2011_COI	KEMA18	1	BC29766_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29766	Sample	BC29766	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA18	COI	45	BC29766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA18	CTTTAATTACAGCGTTTTTATTGTTGT	150.90323
BC29767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46402	WGS84	BC29767	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.642	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29767_Mgig_Bot2012_COI	KEMA17	1	BC29767_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29767	Sample	BC29767	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA17	COI	45	BC29767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA17	TCTTATTCGTTGGAGACTTTATAACCCT	150.90113
BC29768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46436	WGS84	BC29768	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29768_Cmae_Bot2011_COI	KEMA16	1	BC29768_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29768	Sample	BC29768	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA16	COI	45	BC29768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA16	ATGAACAGTCTATCCTCCTTTAG	150.90839
BC29768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46436	WGS84	BC29768	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29768_Msal_Bot2012_COI	KEMA16	1	BC29768_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29768	Sample	BC29768	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA16	COI	45	BC29768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA16	GYTAGTTCCRATGATGTTAGCTG	150.90839
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46527	WGS84	BC29770	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29770_Asen_Wil2025_COI	KEMA13	1	BC29770_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	GTTGTTGTTACAACACATGCTCTTTTG	150.90138
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46527	WGS84	BC29770	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.73	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29770_Mgig_Bot2012_COI	KEMA13	1	BC29770_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	TCTTATTCGTTGGAGACTTTATAACCCT	150.90138
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46606	WGS84	BC29771	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29771_Cmae_Bot2011_COI	KEMA12	1	BC29771_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	ATGAACAGTCTATCCTCCTTTAG	150.90836
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46606	WGS84	BC29771	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29771_Msal_Bot2012_COI	KEMA12	1	BC29771_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	GYTAGTTCCRATGATGTTAGCTG	150.90836
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46723	WGS84	BC29773	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29773_Aamu_Bax2006_COI	KEMA10	1	BC29773_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	GCACAACCGGGATCTTTACTTC	150.90388
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46723	WGS84	BC29773	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29773_Pcan_Bot2011_IGS	KEMA10	1	BC29773_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA10	IGS	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	CGTAATCCTCAGTACTGGCTA	150.90388
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46723	WGS84	BC29773	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29773_Sspa_Oph2007_COI	KEMA10	1	BC29773_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	GAAGGCCTTCCGATGATC	150.90388
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46723	WGS84	BC29773	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29773_Upin_Bot2011_COI	KEMA10	1	BC29773_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	CTTTAATTACAGCGTTTTTATTGTTGT	150.90388
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46723	WGS84	BC29773	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29773_Vgib_Wil2025_COI	KEMA10	1	BC29773_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	CCCCTCTTTACAAGTTCTTTAGGGT	150.90388
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46537	WGS84	BC29774	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29774_Asen_Wil2025_COI	KEMA14	1	BC29774_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	GTTGTTGTTACAACACATGCTCTTTTG	150.90488
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46537	WGS84	BC29774	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.63	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29774_Mgig_Bot2012_COI	KEMA14	1	BC29774_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	TCTTATTCGTTGGAGACTTTATAACCCT	150.90488
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46673	WGS84	BC29775	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29775_Cmae_Bot2011_COI	KEMA9	1	BC29775_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	ATGAACAGTCTATCCTCCTTTAG	150.90128
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46673	WGS84	BC29775	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29775_Msal_Bot2012_COI	KEMA9	1	BC29775_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	GYTAGTTCCRATGATGTTAGCTG	150.90128
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46844	WGS84	BC29777	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29777_Aamu_Bax2006_COI	KEMA7	1	BC29777_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	GCACAACCGGGATCTTTACTTC	150.90496
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46844	WGS84	BC29777	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29777_Pcan_Bot2011_IGS	KEMA7	1	BC29777_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA7	IGS	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	CGTAATCCTCAGTACTGGCTA	150.90496
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46844	WGS84	BC29777	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29777_Sspa_Oph2007_COI	KEMA7	1	BC29777_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	GAAGGCCTTCCGATGATC	150.90496
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46844	WGS84	BC29777	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29777_Upin_Bot2011_COI	KEMA7	1	BC29777_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	CTTTAATTACAGCGTTTTTATTGTTGT	150.90496
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46844	WGS84	BC29777	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29777_Vgib_Wil2025_COI	KEMA7	1	BC29777_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	CCCCTCTTTACAAGTTCTTTAGGGT	150.90496
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.4701	WGS84	BC29778	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29778_Asen_Wil2025_COI	KEMA6	1	BC29778_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	GTTGTTGTTACAACACATGCTCTTTTG	150.9063
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4701	WGS84	BC29778	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.194	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29778_Mgig_Bot2012_COI	KEMA6	1	BC29778_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	TCTTATTCGTTGGAGACTTTATAACCCT	150.9063
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46879	WGS84	BC29779	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29779_Cmae_Bot2011_COI	KEMA5	1	BC29779_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	ATGAACAGTCTATCCTCCTTTAG	150.91175
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46879	WGS84	BC29779	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29779_Msal_Bot2012_COI	KEMA5	1	BC29779_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	GYTAGTTCCRATGATGTTAGCTG	150.91175
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4697	WGS84	BC29780	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29780_Aamu_Bax2006_COI	KEMA4	1	BC29780_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	GCACAACCGGGATCTTTACTTC	150.90884
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4697	WGS84	BC29780	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29780_Pcan_Bot2011_IGS	KEMA4	1	BC29780_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA4	IGS	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	CGTAATCCTCAGTACTGGCTA	150.90884
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4697	WGS84	BC29780	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29780_Sspa_Oph2007_COI	KEMA4	1	BC29780_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	GAAGGCCTTCCGATGATC	150.90884
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4697	WGS84	BC29780	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29780_Upin_Bot2011_COI	KEMA4	1	BC29780_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	CTTTAATTACAGCGTTTTTATTGTTGT	150.90884
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.4697	WGS84	BC29780	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29780_Vgib_Wil2025_COI	KEMA4	1	BC29780_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	CCCCTCTTTACAAGTTCTTTAGGGT	150.90884
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.47125	WGS84	BC29781	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29781_Asen_Wil2025_COI	KEMA3	1	BC29781_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	GTTGTTGTTACAACACATGCTCTTTTG	150.91025
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47125	WGS84	BC29781	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.604	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29781_Mgig_Bot2012_COI	KEMA3	1	BC29781_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	TCTTATTCGTTGGAGACTTTATAACCCT	150.91025
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47213	WGS84	BC29782	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29782_Cmae_Bot2011_COI	KEMA2	1	BC29782_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	ATGAACAGTCTATCCTCCTTTAG	150.91224
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47213	WGS84	BC29782	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29782_Msal_Bot2012_COI	KEMA2	1	BC29782_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	GYTAGTTCCRATGATGTTAGCTG	150.91224
BC29784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96258	WGS84	BC29784	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29784_Aamu_Bax2006_COI	BOTA40	1	BC29784_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29784	Sample	BC29784	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA40	COI	45	BC29784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA40	GCACAACCGGGATCTTTACTTC	151.19841
BC29784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96258	WGS84	BC29784	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29784_Pcan_Bot2011_IGS	BOTA40	1	BC29784_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29784	Sample	BC29784	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA40	IGS	45	BC29784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA40	CGTAATCCTCAGTACTGGCTA	151.19841
BC29784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96258	WGS84	BC29784	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29784_Sspa_Oph2007_COI	BOTA40	1	BC29784_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29784	Sample	BC29784	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA40	COI	45	BC29784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA40	GAAGGCCTTCCGATGATC	151.19841
BC29784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96258	WGS84	BC29784	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29784_Upin_Bot2011_COI	BOTA40	1	BC29784_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29784	Sample	BC29784	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA40	COI	45	BC29784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA40	CTTTAATTACAGCGTTTTTATTGTTGT	151.19841
BC29785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.95907	WGS84	BC29785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.959	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29785_Mgig_Bot2012_COI	BOTA42	1	BC29785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29785	Sample	BC29785	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA42	COI	45	BC29785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA42	TCTTATTCGTTGGAGACTTTATAACCCT	151.1975
BC29786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96074	WGS84	BC29786	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29786_Cmae_Bot2011_COI	BOTA41	1	BC29786_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29786	Sample	BC29786	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA41	COI	45	BC29786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA41	ATGAACAGTCTATCCTCCTTTAG	151.19651
BC29786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96074	WGS84	BC29786	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29786_Msal_Bot2012_COI	BOTA41	1	BC29786_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29786	Sample	BC29786	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA41	COI	45	BC29786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA41	GYTAGTTCCRATGATGTTAGCTG	151.19651
BC29788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96557	WGS84	BC29788	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29788_Aamu_Bax2006_COI	BOTA38	1	BC29788_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29788	Sample	BC29788	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA38	COI	45	BC29788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA38	GCACAACCGGGATCTTTACTTC	151.19871
BC29788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96557	WGS84	BC29788	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29788_Pcan_Bot2011_IGS	BOTA38	1	BC29788_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29788	Sample	BC29788	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA38	IGS	45	BC29788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA38	CGTAATCCTCAGTACTGGCTA	151.19871
BC29788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96557	WGS84	BC29788	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29788_Sspa_Oph2007_COI	BOTA38	1	BC29788_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29788	Sample	BC29788	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA38	COI	45	BC29788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA38	GAAGGCCTTCCGATGATC	151.19871
BC29788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96557	WGS84	BC29788	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29788_Upin_Bot2011_COI	BOTA38	1	BC29788_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29788	Sample	BC29788	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA38	COI	45	BC29788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA38	CTTTAATTACAGCGTTTTTATTGTTGT	151.19871
BC29789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96755	WGS84	BC29789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.664	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29789_Mgig_Bot2012_COI	BOTA37	1	BC29789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29789	Sample	BC29789	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA37	COI	45	BC29789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA37	TCTTATTCGTTGGAGACTTTATAACCCT	151.19821
BC29791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9685	WGS84	BC29791	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29791_Aamu_Bax2006_COI	BOTA35	1	BC29791_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29791	Sample	BC29791	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA35	COI	45	BC29791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA35	GCACAACCGGGATCTTTACTTC	151.2182
BC29791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9685	WGS84	BC29791	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29791_Pcan_Bot2011_IGS	BOTA35	1	BC29791_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29791	Sample	BC29791	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA35	IGS	45	BC29791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA35	CGTAATCCTCAGTACTGGCTA	151.2182
BC29791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9685	WGS84	BC29791	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29791_Sspa_Oph2007_COI	BOTA35	1	BC29791_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29791	Sample	BC29791	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA35	COI	45	BC29791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA35	GAAGGCCTTCCGATGATC	151.2182
BC29791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9685	WGS84	BC29791	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29791_Upin_Bot2011_COI	BOTA35	1	BC29791_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29791	Sample	BC29791	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA35	COI	45	BC29791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA35	CTTTAATTACAGCGTTTTTATTGTTGT	151.2182
BC29792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96878	WGS84	BC29792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.897	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29792_Mgig_Bot2012_COI	BOTA34	1	BC29792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29792	Sample	BC29792	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA34	COI	45	BC29792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA34	TCTTATTCGTTGGAGACTTTATAACCCT	151.21625
BC29793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96896	WGS84	BC29793	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29793_Cmae_Bot2011_COI	BOTA33	1	BC29793_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29793	Sample	BC29793	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA33	COI	45	BC29793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA33	ATGAACAGTCTATCCTCCTTTAG	151.214
BC29793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96896	WGS84	BC29793	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29793_Msal_Bot2012_COI	BOTA33	1	BC29793_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29793	Sample	BC29793	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA33	COI	45	BC29793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA33	GYTAGTTCCRATGATGTTAGCTG	151.214
BC29795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97095	WGS84	BC29795	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29795_Aamu_Bax2006_COI	BOTA31	1	BC29795_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29795	Sample	BC29795	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA31	COI	45	BC29795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA31	GCACAACCGGGATCTTTACTTC	151.19958
BC29795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97095	WGS84	BC29795	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29795_Pcan_Bot2011_IGS	BOTA31	1	BC29795_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29795	Sample	BC29795	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA31	IGS	45	BC29795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA31	CGTAATCCTCAGTACTGGCTA	151.19958
BC29795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97095	WGS84	BC29795	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29795_Sspa_Oph2007_COI	BOTA31	1	BC29795_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29795	Sample	BC29795	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA31	COI	45	BC29795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA31	GAAGGCCTTCCGATGATC	151.19958
BC29795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97095	WGS84	BC29795	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29795_Upin_Bot2011_COI	BOTA31	1	BC29795_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29795	Sample	BC29795	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA31	COI	45	BC29795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA31	CTTTAATTACAGCGTTTTTATTGTTGT	151.19958
BC29796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97016	WGS84	BC29796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.34	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29796_Mgig_Bot2012_COI	BOTA30	1	BC29796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29796	Sample	BC29796	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA30	COI	45	BC29796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA30	TCTTATTCGTTGGAGACTTTATAACCCT	151.21513
BC29797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97063	WGS84	BC29797	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29797_Cmae_Bot2011_COI	BOTA29	1	BC29797_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29797	Sample	BC29797	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA29	COI	45	BC29797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA29	ATGAACAGTCTATCCTCCTTTAG	151.21326
BC29797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97063	WGS84	BC29797	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29797_Msal_Bot2012_COI	BOTA29	1	BC29797_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29797	Sample	BC29797	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA29	COI	45	BC29797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA29	GYTAGTTCCRATGATGTTAGCTG	151.21326
BC29799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9711	WGS84	BC29799	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29799_Aamu_Bax2006_COI	BOTA27	1	BC29799_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29799	Sample	BC29799	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA27	COI	45	BC29799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA27	GCACAACCGGGATCTTTACTTC	151.20951
BC29799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9711	WGS84	BC29799	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29799_Pcan_Bot2011_IGS	BOTA27	1	BC29799_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29799	Sample	BC29799	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA27	IGS	45	BC29799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA27	CGTAATCCTCAGTACTGGCTA	151.20951
BC29799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9711	WGS84	BC29799	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29799_Sspa_Oph2007_COI	BOTA27	1	BC29799_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29799	Sample	BC29799	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA27	COI	45	BC29799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA27	GAAGGCCTTCCGATGATC	151.20951
BC29799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9711	WGS84	BC29799	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29799_Upin_Bot2011_COI	BOTA27	1	BC29799_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29799	Sample	BC29799	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA27	COI	45	BC29799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA27	CTTTAATTACAGCGTTTTTATTGTTGT	151.20951
BC29800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97167	WGS84	BC29800	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.722	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29800_Mgig_Bot2012_COI	BOTA26	1	BC29800_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29800	Sample	BC29800	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA26	COI	45	BC29800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA26	TCTTATTCGTTGGAGACTTTATAACCCT	151.20769
BC29801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97164	WGS84	BC29801	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29801_Cmae_Bot2011_COI	BOTA25	1	BC29801_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29801	Sample	BC29801	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA25	COI	45	BC29801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA25	ATGAACAGTCTATCCTCCTTTAG	151.2054
BC29801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97164	WGS84	BC29801	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29801_Msal_Bot2012_COI	BOTA25	1	BC29801_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29801	Sample	BC29801	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA25	COI	45	BC29801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA25	GYTAGTTCCRATGATGTTAGCTG	151.2054
BC29803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97218	WGS84	BC29803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29803_Aamu_Bax2006_COI	BOTA23	1	BC29803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29803	Sample	BC29803	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA23	COI	45	BC29803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA23	GCACAACCGGGATCTTTACTTC	151.20066
BC29803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97218	WGS84	BC29803	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29803_Pcan_Bot2011_IGS	BOTA23	1	BC29803_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29803	Sample	BC29803	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA23	IGS	45	BC29803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA23	CGTAATCCTCAGTACTGGCTA	151.20066
BC29803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97218	WGS84	BC29803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29803_Sspa_Oph2007_COI	BOTA23	1	BC29803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29803	Sample	BC29803	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA23	COI	45	BC29803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA23	GAAGGCCTTCCGATGATC	151.20066
BC29803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97218	WGS84	BC29803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29803_Upin_Bot2011_COI	BOTA23	1	BC29803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29803	Sample	BC29803	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA23	COI	45	BC29803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA23	CTTTAATTACAGCGTTTTTATTGTTGT	151.20066
BC29804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9725	WGS84	BC29804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.824	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29804_Mgig_Bot2012_COI	BOTA22	1	BC29804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29804	Sample	BC29804	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA22	COI	45	BC29804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA22	TCTTATTCGTTGGAGACTTTATAACCCT	151.21045
BC29805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97277	WGS84	BC29805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29805_Cmae_Bot2011_COI	BOTA21	1	BC29805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29805	Sample	BC29805	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA21	COI	45	BC29805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA21	ATGAACAGTCTATCCTCCTTTAG	151.20822
BC29805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97277	WGS84	BC29805	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29805_Msal_Bot2012_COI	BOTA21	1	BC29805_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29805	Sample	BC29805	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA21	COI	45	BC29805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA21	GYTAGTTCCRATGATGTTAGCTG	151.20822
BC29807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9734	WGS84	BC29807	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29807_Aamu_Bax2006_COI	BOTA19	1	BC29807_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29807	Sample	BC29807	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA19	COI	45	BC29807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA19	GCACAACCGGGATCTTTACTTC	151.20403
BC29807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9734	WGS84	BC29807	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29807_Pcan_Bot2011_IGS	BOTA19	1	BC29807_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29807	Sample	BC29807	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA19	IGS	45	BC29807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA19	CGTAATCCTCAGTACTGGCTA	151.20403
BC29807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9734	WGS84	BC29807	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29807_Sspa_Oph2007_COI	BOTA19	1	BC29807_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29807	Sample	BC29807	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA19	COI	45	BC29807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA19	GAAGGCCTTCCGATGATC	151.20403
BC29807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9734	WGS84	BC29807	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29807_Upin_Bot2011_COI	BOTA19	1	BC29807_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29807	Sample	BC29807	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA19	COI	45	BC29807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA19	CTTTAATTACAGCGTTTTTATTGTTGT	151.20403
BC29808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97356	WGS84	BC29808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.071	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29808_Mgig_Bot2012_COI	BOTA18	1	BC29808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29808	Sample	BC29808	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA18	COI	45	BC29808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA18	TCTTATTCGTTGGAGACTTTATAACCCT	151.2016
BC29809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9739	WGS84	BC29809	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29809_Cmae_Bot2011_COI	BOTA17	1	BC29809_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29809	Sample	BC29809	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA17	COI	45	BC29809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA17	ATGAACAGTCTATCCTCCTTTAG	151.20928
BC29809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9739	WGS84	BC29809	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29809_Msal_Bot2012_COI	BOTA17	1	BC29809_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29809	Sample	BC29809	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA17	COI	45	BC29809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA17	GYTAGTTCCRATGATGTTAGCTG	151.20928
BC29810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.974	WGS84	BC29810	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29810_Aamu_Bax2006_COI	BOTA16	1	BC29810_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29810	Sample	BC29810	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA16	COI	45	BC29810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA16	GCACAACCGGGATCTTTACTTC	151.2075
BC29810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.974	WGS84	BC29810	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29810_Pcan_Bot2011_IGS	BOTA16	1	BC29810_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29810	Sample	BC29810	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA16	IGS	45	BC29810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA16	CGTAATCCTCAGTACTGGCTA	151.2075
BC29810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.974	WGS84	BC29810	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29810_Sspa_Oph2007_COI	BOTA16	1	BC29810_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29810	Sample	BC29810	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA16	COI	45	BC29810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA16	GAAGGCCTTCCGATGATC	151.2075
BC29810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.974	WGS84	BC29810	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29810_Upin_Bot2011_COI	BOTA16	1	BC29810_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29810	Sample	BC29810	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA16	COI	45	BC29810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA16	CTTTAATTACAGCGTTTTTATTGTTGT	151.2075
BC29811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97542	WGS84	BC29811	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.38	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29811_Mgig_Bot2012_COI	BOTA15	1	BC29811_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29811	Sample	BC29811	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA15	COI	45	BC29811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA15	TCTTATTCGTTGGAGACTTTATAACCCT	151.20835
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29812_Cmae_Bot2011_COI	BOTA14	1	BC29812_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	ATGAACAGTCTATCCTCCTTTAG	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29812_Hsan_Wil2021_COI	BOTA14	1	BC29812_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	CCTGGGCCGGTATAGTAGGT	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29812_Mros_Gib2022_COI	BOTA14	1	BC29812_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29812_Msal_Bot2012_COI	BOTA14	1	BC29812_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	GYTAGTTCCRATGATGTTAGCTG	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29812_Pvir_Dia2013_COI	BOTA14	1	BC29812_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	CTTAGTGGCATTAATTCGDAATCC	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29812_Rhar_Sim2018_COI	BOTA14	1	BC29812_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	CTGCTGCTATTGCTCATG	151.20754
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29814_Aamu_Bax2006_COI	BOTA12	1	BC29814_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	GCACAACCGGGATCTTTACTTC	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29814_Pcan_Bot2011_IGS	BOTA12	1	BC29814_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA12	IGS	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	CGTAATCCTCAGTACTGGCTA	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29814_Pper_Dia2013_COI	BOTA12	1	BC29814_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	CTTAGTGGCATTAATTCGDAATCC	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29814_Sspa_Oph2007_COI	BOTA12	1	BC29814_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	GAAGGCCTTCCGATGATC	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29814_Upin_Bot2011_COI	BOTA12	1	BC29814_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	CTTTAATTACAGCGTTTTTATTGTTGT	151.20642
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29815_Cjap_Sim2018_COI	BOTA11	1	BC29815_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29815_Esin_And2018_Cyb	BOTA11	1	BC29815_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA11	Cytb	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	ACCCCTCCTCATATCCAACCA	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.893	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29815_Mgig_Bot2012_COI	BOTA11	1	BC29815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	TCTTATTCGTTGGAGACTTTATAACCCT	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29815_Mjap_Gib2022_COI	BOTA11	1	BC29815_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	AATTTAATTGTGACTAGGCATGGTCTG	151.20777
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29816_Cmae_Bot2011_COI	BOTA10	1	BC29816_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	ATGAACAGTCTATCCTCCTTTAG	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29816_Hsan_Wil2021_COI	BOTA10	1	BC29816_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	CCTGGGCCGGTATAGTAGGT	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29816_Mros_Gib2022_COI	BOTA10	1	BC29816_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29816_Msal_Bot2012_COI	BOTA10	1	BC29816_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	GYTAGTTCCRATGATGTTAGCTG	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29816_Pvir_Dia2013_COI	BOTA10	1	BC29816_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	CTTAGTGGCATTAATTCGDAATCC	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29816_Rhar_Sim2018_COI	BOTA10	1	BC29816_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	CTGCTGCTATTGCTCATG	151.20684
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29818_Aamu_Bax2006_COI	BOTA8	1	BC29818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	GCACAACCGGGATCTTTACTTC	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29818_Pcan_Bot2011_IGS	BOTA8	1	BC29818_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA8	IGS	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	CGTAATCCTCAGTACTGGCTA	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29818_Pper_Dia2013_COI	BOTA8	1	BC29818_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	CTTAGTGGCATTAATTCGDAATCC	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.418	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29818_Sspa_Oph2007_COI	BOTA8	1	BC29818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	GAAGGCCTTCCGATGATC	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29818_Upin_Bot2011_COI	BOTA8	1	BC29818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	CTTTAATTACAGCGTTTTTATTGTTGT	151.20886
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29819_Cjap_Sim2018_COI	BOTA7	1	BC29819_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29819_Esin_And2018_Cyb	BOTA7	1	BC29819_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA7	Cytb	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	ACCCCTCCTCATATCCAACCA	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.825	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29819_Mgig_Bot2012_COI	BOTA7	1	BC29819_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	TCTTATTCGTTGGAGACTTTATAACCCT	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29819_Mjap_Gib2022_COI	BOTA7	1	BC29819_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	AATTTAATTGTGACTAGGCATGGTCTG	151.21359
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29821_Aamu_Bax2006_COI	BOTA5	1	BC29821_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	GCACAACCGGGATCTTTACTTC	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29821_Pcan_Bot2011_IGS	BOTA5	1	BC29821_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA5	IGS	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	CGTAATCCTCAGTACTGGCTA	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29821_Pper_Dia2013_COI	BOTA5	1	BC29821_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	CTTAGTGGCATTAATTCGDAATCC	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.235	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29821_Sspa_Oph2007_COI	BOTA5	1	BC29821_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	GAAGGCCTTCCGATGATC	151.20803
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29822_Cjap_Sim2018_COI	BOTA4	1	BC29822_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29822_Esin_And2018_Cyb	BOTA4	1	BC29822_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA4	Cytb	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	ACCCCTCCTCATATCCAACCA	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.431	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29822_Mgig_Bot2012_COI	BOTA4	1	BC29822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	TCTTATTCGTTGGAGACTTTATAACCCT	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29822_Mjap_Gib2022_COI	BOTA4	1	BC29822_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	AATTTAATTGTGACTAGGCATGGTCTG	151.21232
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29823_Cmae_Bot2011_COI	BOTA3	1	BC29823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	ATGAACAGTCTATCCTCCTTTAG	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29823_Hsan_Wil2021_COI	BOTA3	1	BC29823_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	CCTGGGCCGGTATAGTAGGT	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29823_Mros_Gib2022_COI	BOTA3	1	BC29823_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29823_Msal_Bot2012_COI	BOTA3	1	BC29823_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	GYTAGTTCCRATGATGTTAGCTG	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29823_Pvir_Dia2013_COI	BOTA3	1	BC29823_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	CTTAGTGGCATTAATTCGDAATCC	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29823_Rhar_Sim2018_COI	BOTA3	1	BC29823_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	CTGCTGCTATTGCTCATG	151.20712
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29825_Aamu_Bax2006_COI	BOTA2	1	BC29825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	GCACAACCGGGATCTTTACTTC	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29825_Pcan_Bot2011_IGS	BOTA2	1	BC29825_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA2	IGS	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	CGTAATCCTCAGTACTGGCTA	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29825_Pper_Dia2013_COI	BOTA2	1	BC29825_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	CTTAGTGGCATTAATTCGDAATCC	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29825_Sspa_Oph2007_COI	BOTA2	1	BC29825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	GAAGGCCTTCCGATGATC	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29825_Upin_Bot2011_COI	BOTA2	1	BC29825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	CTTTAATTACAGCGTTTTTATTGTTGT	151.21153
BC29826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66552	WGS84	BC29826	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29826_Mgig_Bot2012_COI	WEIA5	1	BC29826_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29826	Sample	BC29826	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA5	COI	45	BC29826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA5	TCTTATTCGTTGGAGACTTTATAACCCT	141.85005
BC29827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67287	WGS84	BC29827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29827_Cmae_Bot2011_COI	WEIA19	1	BC29827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29827	Sample	BC29827	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA19	COI	45	BC29827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA19	ATGAACAGTCTATCCTCCTTTAG	141.86564
BC29827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67287	WGS84	BC29827	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29827_Msal_Bot2012_COI	WEIA19	1	BC29827_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29827	Sample	BC29827	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA19	COI	45	BC29827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA19	GYTAGTTCCRATGATGTTAGCTG	141.86564
BC29829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67328	WGS84	BC29829	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29829_Aamu_Bax2006_COI	WEIA17	1	BC29829_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29829	Sample	BC29829	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA17	COI	45	BC29829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA17	GCACAACCGGGATCTTTACTTC	141.87218
BC29829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67328	WGS84	BC29829	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29829_Pcan_Bot2011_IGS	WEIA17	1	BC29829_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29829	Sample	BC29829	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA17	IGS	45	BC29829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA17	CGTAATCCTCAGTACTGGCTA	141.87218
BC29829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67328	WGS84	BC29829	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29829_Sspa_Oph2007_COI	WEIA17	1	BC29829_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29829	Sample	BC29829	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA17	COI	45	BC29829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA17	GAAGGCCTTCCGATGATC	141.87218
BC29829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67328	WGS84	BC29829	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29829_Upin_Bot2011_COI	WEIA17	1	BC29829_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29829	Sample	BC29829	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA17	COI	45	BC29829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA17	CTTTAATTACAGCGTTTTTATTGTTGT	141.87218
BC29830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67318	WGS84	BC29830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29830_Cmae_Bot2011_COI	WEIA16	1	BC29830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29830	Sample	BC29830	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA16	COI	45	BC29830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA16	ATGAACAGTCTATCCTCCTTTAG	141.86865
BC29830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67318	WGS84	BC29830	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29830_Msal_Bot2012_COI	WEIA16	1	BC29830_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29830	Sample	BC29830	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA16	COI	45	BC29830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA16	GYTAGTTCCRATGATGTTAGCTG	141.86865
BC29832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67448	WGS84	BC29832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29832_Aamu_Bax2006_COI	WEIA13	1	BC29832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29832	Sample	BC29832	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA13	COI	45	BC29832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA13	GCACAACCGGGATCTTTACTTC	141.87165
BC29832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67448	WGS84	BC29832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29832_Pcan_Bot2011_IGS	WEIA13	1	BC29832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29832	Sample	BC29832	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA13	IGS	45	BC29832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA13	CGTAATCCTCAGTACTGGCTA	141.87165
BC29832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67448	WGS84	BC29832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29832_Sspa_Oph2007_COI	WEIA13	1	BC29832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29832	Sample	BC29832	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA13	COI	45	BC29832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA13	GAAGGCCTTCCGATGATC	141.87165
BC29832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67448	WGS84	BC29832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29832_Upin_Bot2011_COI	WEIA13	1	BC29832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29832	Sample	BC29832	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA13	COI	45	BC29832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA13	CTTTAATTACAGCGTTTTTATTGTTGT	141.87165
BC29833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67486	WGS84	BC29833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29833_Mgig_Bot2012_COI	WEIA12	1	BC29833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29833	Sample	BC29833	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA12	COI	45	BC29833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA12	TCTTATTCGTTGGAGACTTTATAACCCT	141.87001
BC29834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67501	WGS84	BC29834	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29834_Cmae_Bot2011_COI	WEIA11	1	BC29834_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29834	Sample	BC29834	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA11	COI	45	BC29834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA11	ATGAACAGTCTATCCTCCTTTAG	141.86849
BC29834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67501	WGS84	BC29834	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29834_Msal_Bot2012_COI	WEIA11	1	BC29834_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29834	Sample	BC29834	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA11	COI	45	BC29834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA11	GYTAGTTCCRATGATGTTAGCTG	141.86849
BC29836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66946	WGS84	BC29836	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29836_Aamu_Bax2006_COI	WEIA8	1	BC29836_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29836	Sample	BC29836	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA8	COI	45	BC29836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA8	GCACAACCGGGATCTTTACTTC	141.86323
BC29836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66946	WGS84	BC29836	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29836_Pcan_Bot2011_IGS	WEIA8	1	BC29836_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29836	Sample	BC29836	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA8	IGS	45	BC29836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA8	CGTAATCCTCAGTACTGGCTA	141.86323
BC29836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66946	WGS84	BC29836	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29836_Sspa_Oph2007_COI	WEIA8	1	BC29836_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29836	Sample	BC29836	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA8	COI	45	BC29836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA8	GAAGGCCTTCCGATGATC	141.86323
BC29836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66946	WGS84	BC29836	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29836_Upin_Bot2011_COI	WEIA8	1	BC29836_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29836	Sample	BC29836	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA8	COI	45	BC29836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA8	CTTTAATTACAGCGTTTTTATTGTTGT	141.86323
BC29837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67017	WGS84	BC29837	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29837_Mgig_Bot2012_COI	WEIA7	1	BC29837_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29837	Sample	BC29837	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA7	COI	45	BC29837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA7	TCTTATTCGTTGGAGACTTTATAACCCT	141.86209
BC29838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66604	WGS84	BC29838	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29838_Cmae_Bot2011_COI	WEIA4	1	BC29838_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29838	Sample	BC29838	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA4	COI	45	BC29838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA4	ATGAACAGTCTATCCTCCTTTAG	141.84772
BC29838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66604	WGS84	BC29838	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29838_Msal_Bot2012_COI	WEIA4	1	BC29838_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29838	Sample	BC29838	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA4	COI	45	BC29838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA4	GYTAGTTCCRATGATGTTAGCTG	141.84772
BC29840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.6676	WGS84	BC29840	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29840_Mgig_Bot2012_COI	WEIA1	1	BC29840_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29840	Sample	BC29840	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA1	COI	45	BC29840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA1	TCTTATTCGTTGGAGACTTTATAACCCT	141.846
BC29847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20909	WGS84	BC29847	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29847_Aamu_Bax2006_COI	GOVA10	1	BC29847_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29847	Sample	BC29847	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA10	COI	45	BC29847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA10	GCACAACCGGGATCTTTACTTC	136.66891
BC29847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20909	WGS84	BC29847	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29847_Pcan_Bot2011_IGS	GOVA10	1	BC29847_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29847	Sample	BC29847	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA10	IGS	45	BC29847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA10	CGTAATCCTCAGTACTGGCTA	136.66891
BC29847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20909	WGS84	BC29847	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29847_Sspa_Oph2007_COI	GOVA10	1	BC29847_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29847	Sample	BC29847	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA10	COI	45	BC29847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA10	GAAGGCCTTCCGATGATC	136.66891
BC29847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20909	WGS84	BC29847	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29847_Upin_Bot2011_COI	GOVA10	1	BC29847_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29847	Sample	BC29847	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA10	COI	45	BC29847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA10	CTTTAATTACAGCGTTTTTATTGTTGT	136.66891
BC29848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20461	WGS84	BC29848	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29848_Mgig_Bot2012_COI	GOVA5	1	BC29848_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29848	Sample	BC29848	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA5	COI	45	BC29848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA5	TCTTATTCGTTGGAGACTTTATAACCCT	136.68266
BC29849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20293	WGS84	BC29849	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29849_Cmae_Bot2011_COI	GOVA22	1	BC29849_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29849	Sample	BC29849	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA22	COI	45	BC29849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA22	ATGAACAGTCTATCCTCCTTTAG	136.66973
BC29849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20293	WGS84	BC29849	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29849_Msal_Bot2012_COI	GOVA22	1	BC29849_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29849	Sample	BC29849	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA22	COI	45	BC29849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA22	GYTAGTTCCRATGATGTTAGCTG	136.66973
BC29850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20397	WGS84	BC29850	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29850_Aamu_Bax2006_COI	GOVA21	1	BC29850_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29850	Sample	BC29850	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA21	COI	45	BC29850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA21	GCACAACCGGGATCTTTACTTC	136.67036
BC29850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20397	WGS84	BC29850	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29850_Pcan_Bot2011_IGS	GOVA21	1	BC29850_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29850	Sample	BC29850	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA21	IGS	45	BC29850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA21	CGTAATCCTCAGTACTGGCTA	136.67036
BC29850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20397	WGS84	BC29850	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29850_Sspa_Oph2007_COI	GOVA21	1	BC29850_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29850	Sample	BC29850	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA21	COI	45	BC29850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA21	GAAGGCCTTCCGATGATC	136.67036
BC29850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20397	WGS84	BC29850	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29850_Upin_Bot2011_COI	GOVA21	1	BC29850_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29850	Sample	BC29850	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA21	COI	45	BC29850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA21	CTTTAATTACAGCGTTTTTATTGTTGT	136.67036
BC29851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20509	WGS84	BC29851	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29851_Mgig_Bot2012_COI	GOVA19	1	BC29851_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29851	Sample	BC29851	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA19	COI	45	BC29851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA19	TCTTATTCGTTGGAGACTTTATAACCCT	136.66844
BC29852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20625	WGS84	BC29852	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29852_Cmae_Bot2011_COI	GOVA17	1	BC29852_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29852	Sample	BC29852	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA17	COI	45	BC29852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA17	ATGAACAGTCTATCCTCCTTTAG	136.6721
BC29852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20625	WGS84	BC29852	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29852_Msal_Bot2012_COI	GOVA17	1	BC29852_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29852	Sample	BC29852	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA17	COI	45	BC29852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA17	GYTAGTTCCRATGATGTTAGCTG	136.6721
BC29854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20891	WGS84	BC29854	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29854_Aamu_Bax2006_COI	GOVA11	1	BC29854_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29854	Sample	BC29854	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA11	COI	45	BC29854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA11	GCACAACCGGGATCTTTACTTC	136.67007
BC29854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20891	WGS84	BC29854	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29854_Pcan_Bot2011_IGS	GOVA11	1	BC29854_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29854	Sample	BC29854	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA11	IGS	45	BC29854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA11	CGTAATCCTCAGTACTGGCTA	136.67007
BC29854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20891	WGS84	BC29854	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29854_Sspa_Oph2007_COI	GOVA11	1	BC29854_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29854	Sample	BC29854	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA11	COI	45	BC29854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA11	GAAGGCCTTCCGATGATC	136.67007
BC29854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20891	WGS84	BC29854	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29854_Upin_Bot2011_COI	GOVA11	1	BC29854_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29854	Sample	BC29854	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA11	COI	45	BC29854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA11	CTTTAATTACAGCGTTTTTATTGTTGT	136.67007
BC29855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.21015	WGS84	BC29855	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29855_Mgig_Bot2012_COI	GOVA9	1	BC29855_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29855	Sample	BC29855	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA9	COI	45	BC29855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA9	TCTTATTCGTTGGAGACTTTATAACCCT	136.67167
BC29856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20147	WGS84	BC29856	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29856_Cmae_Bot2011_COI	GOVA7	1	BC29856_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29856	Sample	BC29856	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA7	COI	45	BC29856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA7	ATGAACAGTCTATCCTCCTTTAG	136.68073
BC29856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20147	WGS84	BC29856	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29856_Msal_Bot2012_COI	GOVA7	1	BC29856_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29856	Sample	BC29856	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA7	COI	45	BC29856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA7	GYTAGTTCCRATGATGTTAGCTG	136.68073
BC29858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20648	WGS84	BC29858	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29858_Aamu_Bax2006_COI	GOVA3	1	BC29858_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29858	Sample	BC29858	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA3	COI	45	BC29858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA3	GCACAACCGGGATCTTTACTTC	136.68
BC29858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20648	WGS84	BC29858	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29858_Pcan_Bot2011_IGS	GOVA3	1	BC29858_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29858	Sample	BC29858	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA3	IGS	45	BC29858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA3	CGTAATCCTCAGTACTGGCTA	136.68
BC29858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20648	WGS84	BC29858	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29858_Sspa_Oph2007_COI	GOVA3	1	BC29858_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29858	Sample	BC29858	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA3	COI	45	BC29858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA3	GAAGGCCTTCCGATGATC	136.68
BC29858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20648	WGS84	BC29858	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29858_Upin_Bot2011_COI	GOVA3	1	BC29858_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29858	Sample	BC29858	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA3	COI	45	BC29858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA3	CTTTAATTACAGCGTTTTTATTGTTGT	136.68
BC29859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19561	WGS84	BC29859	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29859_Mgig_Bot2012_COI	GOVA2	1	BC29859_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29859	Sample	BC29859	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA2	COI	45	BC29859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA2	TCTTATTCGTTGGAGACTTTATAACCCT	136.68673
BC37387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99876	WGS84	BC37387	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37387_Cmae_Bot2011_COI	BOTB6	1	BC37387_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37387	Sample	BC37387	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB6	COI	45	BC37387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB6	ATGAACAGTCTATCCTCCTTTAG	151.2101
BC37387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99876	WGS84	BC37387	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37387_Msal_Bot2012_COI	BOTB6	1	BC37387_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37387	Sample	BC37387	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB6	COI	45	BC37387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB6	GYTAGTTCCRATGATGTTAGCTG	151.2101
BC37389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99727	WGS84	BC37389	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37389_Aamu_Bax2006_COI	BOTB8	1	BC37389_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37389	Sample	BC37389	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB8	COI	45	BC37389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB8	GCACAACCGGGATCTTTACTTC	151.20967
BC37389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99727	WGS84	BC37389	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37389_Pcan_Bot2011_IGS	BOTB8	1	BC37389_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37389	Sample	BC37389	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB8	IGS	45	BC37389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB8	CGTAATCCTCAGTACTGGCTA	151.20967
BC37389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99727	WGS84	BC37389	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37389_Sspa_Oph2007_COI	BOTB8	1	BC37389_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37389	Sample	BC37389	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB8	COI	45	BC37389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB8	GAAGGCCTTCCGATGATC	151.20967
BC37389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99727	WGS84	BC37389	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37389_Upin_Bot2011_COI	BOTB8	1	BC37389_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37389	Sample	BC37389	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB8	COI	45	BC37389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB8	CTTTAATTACAGCGTTTTTATTGTTGT	151.20967
BC37390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99718	WGS84	BC37390	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37390_Cmae_Bot2011_COI	BOTB9	1	BC37390_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37390	Sample	BC37390	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB9	COI	45	BC37390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB9	ATGAACAGTCTATCCTCCTTTAG	151.21115
BC37390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99718	WGS84	BC37390	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37390_Msal_Bot2012_COI	BOTB9	1	BC37390_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37390	Sample	BC37390	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB9	COI	45	BC37390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB9	GYTAGTTCCRATGATGTTAGCTG	151.21115
BC37392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98116	WGS84	BC37392	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37392_Aamu_Bax2006_COI	BOTB11	1	BC37392_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37392	Sample	BC37392	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB11	COI	45	BC37392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB11	GCACAACCGGGATCTTTACTTC	151.20848
BC37392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98116	WGS84	BC37392	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37392_Pcan_Bot2011_IGS	BOTB11	1	BC37392_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37392	Sample	BC37392	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB11	IGS	45	BC37392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB11	CGTAATCCTCAGTACTGGCTA	151.20848
BC37392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98116	WGS84	BC37392	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37392_Sspa_Oph2007_COI	BOTB11	1	BC37392_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37392	Sample	BC37392	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB11	COI	45	BC37392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB11	GAAGGCCTTCCGATGATC	151.20848
BC37392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98116	WGS84	BC37392	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37392_Upin_Bot2011_COI	BOTB11	1	BC37392_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37392	Sample	BC37392	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB11	COI	45	BC37392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB11	CTTTAATTACAGCGTTTTTATTGTTGT	151.20848
BC37393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97923	WGS84	BC37393	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37393_Mgig_Bot2012_COI	BOTB12	1	BC37393_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37393	Sample	BC37393	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB12	COI	45	BC37393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB12	TCTTATTCGTTGGAGACTTTATAACCCT	151.20787
BC37394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97853	WGS84	BC37394	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37394_Cmae_Bot2011_COI	BOTB13	1	BC37394_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37394	Sample	BC37394	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB13	COI	45	BC37394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB13	ATGAACAGTCTATCCTCCTTTAG	151.20963
BC37394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97853	WGS84	BC37394	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37394_Msal_Bot2012_COI	BOTB13	1	BC37394_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37394	Sample	BC37394	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB13	COI	45	BC37394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB13	GYTAGTTCCRATGATGTTAGCTG	151.20963
BC37396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97617	WGS84	BC37396	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37396_Aamu_Bax2006_COI	BOTB15	1	BC37396_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37396	Sample	BC37396	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB15	COI	45	BC37396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB15	GCACAACCGGGATCTTTACTTC	151.20929
BC37396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97617	WGS84	BC37396	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37396_Pcan_Bot2011_IGS	BOTB15	1	BC37396_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37396	Sample	BC37396	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB15	IGS	45	BC37396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB15	CGTAATCCTCAGTACTGGCTA	151.20929
BC37396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97617	WGS84	BC37396	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37396_Sspa_Oph2007_COI	BOTB15	1	BC37396_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37396	Sample	BC37396	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB15	COI	45	BC37396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB15	GAAGGCCTTCCGATGATC	151.20929
BC37396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97617	WGS84	BC37396	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37396_Upin_Bot2011_COI	BOTB15	1	BC37396_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37396	Sample	BC37396	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB15	COI	45	BC37396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB15	CTTTAATTACAGCGTTTTTATTGTTGT	151.20929
BC37397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97578	WGS84	BC37397	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37397_Mgig_Bot2012_COI	BOTB16	1	BC37397_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37397	Sample	BC37397	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB16	COI	45	BC37397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB16	TCTTATTCGTTGGAGACTTTATAACCCT	151.2104
BC37398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97457	WGS84	BC37398	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37398_Cmae_Bot2011_COI	BOTB17	1	BC37398_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37398	Sample	BC37398	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB17	COI	45	BC37398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB17	ATGAACAGTCTATCCTCCTTTAG	151.2098
BC37398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97457	WGS84	BC37398	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37398_Msal_Bot2012_COI	BOTB17	1	BC37398_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37398	Sample	BC37398	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB17	COI	45	BC37398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB17	GYTAGTTCCRATGATGTTAGCTG	151.2098
BC37400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97257	WGS84	BC37400	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37400_Aamu_Bax2006_COI	BOTB19	1	BC37400_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37400	Sample	BC37400	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB19	COI	45	BC37400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB19	GCACAACCGGGATCTTTACTTC	151.20432
BC37400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97257	WGS84	BC37400	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37400_Pcan_Bot2011_IGS	BOTB19	1	BC37400_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37400	Sample	BC37400	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB19	IGS	45	BC37400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB19	CGTAATCCTCAGTACTGGCTA	151.20432
BC37400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97257	WGS84	BC37400	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.3	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37400_Sspa_Oph2007_COI	BOTB19	1	BC37400_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37400	Sample	BC37400	targeted	Present	Sample	Sspa_Oph2007_COI	BOTB19	COI	45	BC37400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB19	GAAGGCCTTCCGATGATC	151.20432
BC37400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97257	WGS84	BC37400	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37400_Upin_Bot2011_COI	BOTB19	1	BC37400_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37400	Sample	BC37400	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB19	COI	45	BC37400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB19	CTTTAATTACAGCGTTTTTATTGTTGT	151.20432
BC37401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97244	WGS84	BC37401	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37401_Mgig_Bot2012_COI	BOTB20	1	BC37401_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37401	Sample	BC37401	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB20	COI	45	BC37401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB20	TCTTATTCGTTGGAGACTTTATAACCCT	151.20641
BC37402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97291	WGS84	BC37402	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37402_Cmae_Bot2011_COI	BOTB21	1	BC37402_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37402	Sample	BC37402	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB21	COI	45	BC37402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB21	ATGAACAGTCTATCCTCCTTTAG	151.20878
BC37402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97291	WGS84	BC37402	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37402_Msal_Bot2012_COI	BOTB21	1	BC37402_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37402	Sample	BC37402	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB21	COI	45	BC37402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB21	GYTAGTTCCRATGATGTTAGCTG	151.20878
BC37404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97176	WGS84	BC37404	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37404_Aamu_Bax2006_COI	BOTB23	1	BC37404_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37404	Sample	BC37404	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB23	COI	45	BC37404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB23	GCACAACCGGGATCTTTACTTC	151.19977
BC37404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97176	WGS84	BC37404	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37404_Pcan_Bot2011_IGS	BOTB23	1	BC37404_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37404	Sample	BC37404	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB23	IGS	45	BC37404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB23	CGTAATCCTCAGTACTGGCTA	151.19977
BC37404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97176	WGS84	BC37404	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37404_Sspa_Oph2007_COI	BOTB23	1	BC37404_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37404	Sample	BC37404	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB23	COI	45	BC37404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB23	GAAGGCCTTCCGATGATC	151.19977
BC37404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97176	WGS84	BC37404	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37404_Upin_Bot2011_COI	BOTB23	1	BC37404_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37404	Sample	BC37404	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB23	COI	45	BC37404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB23	CTTTAATTACAGCGTTTTTATTGTTGT	151.19977
BC37405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97152	WGS84	BC37405	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37405_Mgig_Bot2012_COI	BOTB24	1	BC37405_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37405	Sample	BC37405	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB24	COI	45	BC37405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB24	TCTTATTCGTTGGAGACTTTATAACCCT	151.20333
BC37406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9714	WGS84	BC37406	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37406_Cmae_Bot2011_COI	BOTB25	1	BC37406_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37406	Sample	BC37406	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB25	COI	45	BC37406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB25	ATGAACAGTCTATCCTCCTTTAG	151.20539
BC37406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9714	WGS84	BC37406	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37406_Msal_Bot2012_COI	BOTB25	1	BC37406_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37406	Sample	BC37406	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB25	COI	45	BC37406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB25	GYTAGTTCCRATGATGTTAGCTG	151.20539
BC37408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97067	WGS84	BC37408	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37408_Aamu_Bax2006_COI	BOTB27	1	BC37408_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37408	Sample	BC37408	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB27	COI	45	BC37408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB27	GCACAACCGGGATCTTTACTTC	151.20974
BC37408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97067	WGS84	BC37408	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37408_Pcan_Bot2011_IGS	BOTB27	1	BC37408_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37408	Sample	BC37408	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB27	IGS	45	BC37408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB27	CGTAATCCTCAGTACTGGCTA	151.20974
BC37408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97067	WGS84	BC37408	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.354	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37408_Sspa_Oph2007_COI	BOTB27	1	BC37408_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37408	Sample	BC37408	targeted	Present	Sample	Sspa_Oph2007_COI	BOTB27	COI	45	BC37408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB27	GAAGGCCTTCCGATGATC	151.20974
BC37408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97067	WGS84	BC37408	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37408_Upin_Bot2011_COI	BOTB27	1	BC37408_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37408	Sample	BC37408	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB27	COI	45	BC37408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB27	CTTTAATTACAGCGTTTTTATTGTTGT	151.20974
BC37409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97049	WGS84	BC37409	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37409_Mgig_Bot2012_COI	BOTB28	1	BC37409_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37409	Sample	BC37409	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB28	COI	45	BC37409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB28	TCTTATTCGTTGGAGACTTTATAACCCT	151.21199
BC37411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96986	WGS84	BC37411	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37411_Aamu_Bax2006_COI	BOTB30	1	BC37411_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37411	Sample	BC37411	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB30	COI	45	BC37411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB30	GCACAACCGGGATCTTTACTTC	151.21632
BC37411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96986	WGS84	BC37411	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37411_Pcan_Bot2011_IGS	BOTB30	1	BC37411_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37411	Sample	BC37411	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB30	IGS	45	BC37411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB30	CGTAATCCTCAGTACTGGCTA	151.21632
BC37411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96986	WGS84	BC37411	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37411_Sspa_Oph2007_COI	BOTB30	1	BC37411_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37411	Sample	BC37411	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB30	COI	45	BC37411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB30	GAAGGCCTTCCGATGATC	151.21632
BC37411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96986	WGS84	BC37411	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37411_Upin_Bot2011_COI	BOTB30	1	BC37411_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37411	Sample	BC37411	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB30	COI	45	BC37411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB30	CTTTAATTACAGCGTTTTTATTGTTGT	151.21632
BC37412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97019	WGS84	BC37412	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37412_Mgig_Bot2012_COI	BOTB31	1	BC37412_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37412	Sample	BC37412	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB31	COI	45	BC37412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB31	TCTTATTCGTTGGAGACTTTATAACCCT	151.19852
BC37413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97061	WGS84	BC37413	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37413_Cmae_Bot2011_COI	BOTB32	1	BC37413_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37413	Sample	BC37413	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB32	COI	45	BC37413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB32	ATGAACAGTCTATCCTCCTTTAG	151.20139
BC37413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97061	WGS84	BC37413	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37413_Msal_Bot2012_COI	BOTB32	1	BC37413_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37413	Sample	BC37413	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB32	COI	45	BC37413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB32	GYTAGTTCCRATGATGTTAGCTG	151.20139
BC37415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9682	WGS84	BC37415	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37415_Aamu_Bax2006_COI	BOTB34	1	BC37415_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37415	Sample	BC37415	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB34	COI	45	BC37415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB34	GCACAACCGGGATCTTTACTTC	151.21721
BC37415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9682	WGS84	BC37415	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37415_Pcan_Bot2011_IGS	BOTB34	1	BC37415_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37415	Sample	BC37415	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB34	IGS	45	BC37415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB34	CGTAATCCTCAGTACTGGCTA	151.21721
BC37415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9682	WGS84	BC37415	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37415_Sspa_Oph2007_COI	BOTB34	1	BC37415_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37415	Sample	BC37415	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB34	COI	45	BC37415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB34	GAAGGCCTTCCGATGATC	151.21721
BC37415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9682	WGS84	BC37415	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37415_Upin_Bot2011_COI	BOTB34	1	BC37415_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37415	Sample	BC37415	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB34	COI	45	BC37415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB34	CTTTAATTACAGCGTTTTTATTGTTGT	151.21721
BC37416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96834	WGS84	BC37416	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37416_Mgig_Bot2012_COI	BOTB35	1	BC37416_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37416	Sample	BC37416	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB35	COI	45	BC37416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB35	TCTTATTCGTTGGAGACTTTATAACCCT	151.21836
BC37417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9683	WGS84	BC37417	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37417_Cmae_Bot2011_COI	BOTB36	1	BC37417_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37417	Sample	BC37417	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB36	COI	45	BC37417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB36	ATGAACAGTCTATCCTCCTTTAG	151.19945
BC37417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9683	WGS84	BC37417	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37417_Msal_Bot2012_COI	BOTB36	1	BC37417_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37417	Sample	BC37417	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB36	COI	45	BC37417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB36	GYTAGTTCCRATGATGTTAGCTG	151.19945
BC37419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96582	WGS84	BC37419	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37419_Aamu_Bax2006_COI	BOTB38	1	BC37419_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37419	Sample	BC37419	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB38	COI	45	BC37419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB38	GCACAACCGGGATCTTTACTTC	151.19983
BC37419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96582	WGS84	BC37419	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37419_Pcan_Bot2011_IGS	BOTB38	1	BC37419_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37419	Sample	BC37419	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB38	IGS	45	BC37419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB38	CGTAATCCTCAGTACTGGCTA	151.19983
BC37419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96582	WGS84	BC37419	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37419_Sspa_Oph2007_COI	BOTB38	1	BC37419_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37419	Sample	BC37419	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB38	COI	45	BC37419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB38	GAAGGCCTTCCGATGATC	151.19983
BC37419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96582	WGS84	BC37419	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37419_Upin_Bot2011_COI	BOTB38	1	BC37419_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37419	Sample	BC37419	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB38	COI	45	BC37419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB38	CTTTAATTACAGCGTTTTTATTGTTGT	151.19983
BC37420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96368	WGS84	BC37420	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37420_Cmae_Bot2011_COI	BOTB39	1	BC37420_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37420	Sample	BC37420	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB39	COI	45	BC37420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB39	ATGAACAGTCTATCCTCCTTTAG	151.19844
BC37420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96368	WGS84	BC37420	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37420_Msal_Bot2012_COI	BOTB39	1	BC37420_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37420	Sample	BC37420	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB39	COI	45	BC37420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB39	GYTAGTTCCRATGATGTTAGCTG	151.19844
BC37422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96071	WGS84	BC37422	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37422_Aamu_Bax2006_COI	BOTB41	1	BC37422_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37422	Sample	BC37422	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB41	COI	45	BC37422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB41	GCACAACCGGGATCTTTACTTC	151.19803
BC37422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96071	WGS84	BC37422	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37422_Pcan_Bot2011_IGS	BOTB41	1	BC37422_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37422	Sample	BC37422	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB41	IGS	45	BC37422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB41	CGTAATCCTCAGTACTGGCTA	151.19803
BC37422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96071	WGS84	BC37422	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37422_Sspa_Oph2007_COI	BOTB41	1	BC37422_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37422	Sample	BC37422	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB41	COI	45	BC37422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB41	GAAGGCCTTCCGATGATC	151.19803
BC37422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96071	WGS84	BC37422	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37422_Upin_Bot2011_COI	BOTB41	1	BC37422_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37422	Sample	BC37422	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB41	COI	45	BC37422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB41	CTTTAATTACAGCGTTTTTATTGTTGT	151.19803
BC37424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96267	WGS84	BC37424	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37424_Cmae_Bot2011_COI	BOTB40	1	BC37424_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37424	Sample	BC37424	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB40	COI	45	BC37424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB40	ATGAACAGTCTATCCTCCTTTAG	151.19922
BC37424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96267	WGS84	BC37424	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37424_Msal_Bot2012_COI	BOTB40	1	BC37424_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37424	Sample	BC37424	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB40	COI	45	BC37424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB40	GYTAGTTCCRATGATGTTAGCTG	151.19922
BC37426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8814	WGS84	BC37426	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37426_Aamu_Bax2006_COI	NEWB46	1	BC37426_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37426	Sample	BC37426	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB46	COI	45	BC37426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB46	GCACAACCGGGATCTTTACTTC	151.75162
BC37426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8814	WGS84	BC37426	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37426_Pcan_Bot2011_IGS	NEWB46	1	BC37426_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37426	Sample	BC37426	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB46	IGS	45	BC37426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB46	CGTAATCCTCAGTACTGGCTA	151.75162
BC37426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8814	WGS84	BC37426	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37426_Sspa_Oph2007_COI	NEWB46	1	BC37426_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37426	Sample	BC37426	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB46	COI	45	BC37426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB46	GAAGGCCTTCCGATGATC	151.75162
BC37426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8814	WGS84	BC37426	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37426_Upin_Bot2011_COI	NEWB46	1	BC37426_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37426	Sample	BC37426	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB46	COI	45	BC37426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB46	CTTTAATTACAGCGTTTTTATTGTTGT	151.75162
BC37427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88148	WGS84	BC37427	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.317	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37427_Mgig_Bot2012_COI	NEWB45	1	BC37427_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37427	Sample	BC37427	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB45	COI	45	BC37427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB45	TCTTATTCGTTGGAGACTTTATAACCCT	151.74943
BC37428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88286	WGS84	BC37428	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37428_Cmae_Bot2011_COI	NEWB44	1	BC37428_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37428	Sample	BC37428	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB44	COI	45	BC37428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB44	ATGAACAGTCTATCCTCCTTTAG	151.75313
BC37428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88286	WGS84	BC37428	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37428_Msal_Bot2012_COI	NEWB44	1	BC37428_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37428	Sample	BC37428	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB44	COI	45	BC37428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB44	GYTAGTTCCRATGATGTTAGCTG	151.75313
BC37430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88439	WGS84	BC37430	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37430_Mgig_Bot2012_COI	NEWB42	1	BC37430_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37430	Sample	BC37430	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB42	COI	45	BC37430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB42	TCTTATTCGTTGGAGACTTTATAACCCT	151.75453
BC37431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88566	WGS84	BC37431	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37431_Cmae_Bot2011_COI	NEWB41	1	BC37431_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37431	Sample	BC37431	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB41	COI	45	BC37431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB41	ATGAACAGTCTATCCTCCTTTAG	151.75829
BC37431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88566	WGS84	BC37431	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37431_Msal_Bot2012_COI	NEWB41	1	BC37431_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37431	Sample	BC37431	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB41	COI	45	BC37431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB41	GYTAGTTCCRATGATGTTAGCTG	151.75829
BC37433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88695	WGS84	BC37433	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37433_Aamu_Bax2006_COI	NEWB39	1	BC37433_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37433	Sample	BC37433	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB39	COI	45	BC37433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB39	GCACAACCGGGATCTTTACTTC	151.76199
BC37433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88695	WGS84	BC37433	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37433_Pcan_Bot2011_IGS	NEWB39	1	BC37433_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37433	Sample	BC37433	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB39	IGS	45	BC37433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB39	CGTAATCCTCAGTACTGGCTA	151.76199
BC37433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88695	WGS84	BC37433	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37433_Sspa_Oph2007_COI	NEWB39	1	BC37433_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37433	Sample	BC37433	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB39	COI	45	BC37433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB39	GAAGGCCTTCCGATGATC	151.76199
BC37433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88695	WGS84	BC37433	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37433_Upin_Bot2011_COI	NEWB39	1	BC37433_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37433	Sample	BC37433	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB39	COI	45	BC37433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB39	CTTTAATTACAGCGTTTTTATTGTTGT	151.76199
BC37434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8867	WGS84	BC37434	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.041	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37434_Mgig_Bot2012_COI	NEWB38	1	BC37434_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37434	Sample	BC37434	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB38	COI	45	BC37434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB38	TCTTATTCGTTGGAGACTTTATAACCCT	151.75959
BC37435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88803	WGS84	BC37435	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37435_Cmae_Bot2011_COI	NEWB37	1	BC37435_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37435	Sample	BC37435	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB37	COI	45	BC37435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB37	ATGAACAGTCTATCCTCCTTTAG	151.76579
BC37435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88803	WGS84	BC37435	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37435_Msal_Bot2012_COI	NEWB37	1	BC37435_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37435	Sample	BC37435	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB37	COI	45	BC37435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB37	GYTAGTTCCRATGATGTTAGCTG	151.76579
BC37437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8881	WGS84	BC37437	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37437_Aamu_Bax2006_COI	NEWB35	1	BC37437_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37437	Sample	BC37437	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB35	COI	45	BC37437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB35	GCACAACCGGGATCTTTACTTC	151.7608
BC37437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8881	WGS84	BC37437	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37437_Pcan_Bot2011_IGS	NEWB35	1	BC37437_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37437	Sample	BC37437	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB35	IGS	45	BC37437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB35	CGTAATCCTCAGTACTGGCTA	151.7608
BC37437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8881	WGS84	BC37437	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37437_Sspa_Oph2007_COI	NEWB35	1	BC37437_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37437	Sample	BC37437	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB35	COI	45	BC37437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB35	GAAGGCCTTCCGATGATC	151.7608
BC37437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8881	WGS84	BC37437	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37437_Upin_Bot2011_COI	NEWB35	1	BC37437_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37437	Sample	BC37437	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB35	COI	45	BC37437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB35	CTTTAATTACAGCGTTTTTATTGTTGT	151.7608
BC37438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88925	WGS84	BC37438	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37438_Mgig_Bot2012_COI	NEWB34	1	BC37438_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37438	Sample	BC37438	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB34	COI	45	BC37438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB34	TCTTATTCGTTGGAGACTTTATAACCCT	151.76953
BC37439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88954	WGS84	BC37439	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37439_Cmae_Bot2011_COI	NEWB33	1	BC37439_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37439	Sample	BC37439	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB33	COI	45	BC37439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB33	ATGAACAGTCTATCCTCCTTTAG	151.76725
BC37439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88954	WGS84	BC37439	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37439_Msal_Bot2012_COI	NEWB33	1	BC37439_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37439	Sample	BC37439	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB33	COI	45	BC37439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB33	GYTAGTTCCRATGATGTTAGCTG	151.76725
BC37440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88948	WGS84	BC37440	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37440_Aamu_Bax2006_COI	NEWB32	1	BC37440_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37440	Sample	BC37440	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB32	COI	45	BC37440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB32	GCACAACCGGGATCTTTACTTC	151.76464
BC37440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88948	WGS84	BC37440	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37440_Pcan_Bot2011_IGS	NEWB32	1	BC37440_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37440	Sample	BC37440	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB32	IGS	45	BC37440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB32	CGTAATCCTCAGTACTGGCTA	151.76464
BC37440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88948	WGS84	BC37440	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37440_Sspa_Oph2007_COI	NEWB32	1	BC37440_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37440	Sample	BC37440	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB32	COI	45	BC37440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB32	GAAGGCCTTCCGATGATC	151.76464
BC37440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88948	WGS84	BC37440	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37440_Upin_Bot2011_COI	NEWB32	1	BC37440_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37440	Sample	BC37440	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB32	COI	45	BC37440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB32	CTTTAATTACAGCGTTTTTATTGTTGT	151.76464
BC37441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89028	WGS84	BC37441	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.251	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37441_Mgig_Bot2012_COI	NEWB31	1	BC37441_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37441	Sample	BC37441	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB31	COI	45	BC37441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB31	TCTTATTCGTTGGAGACTTTATAACCCT	151.77213
BC37442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89074	WGS84	BC37442	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37442_Cmae_Bot2011_COI	NEWB30	1	BC37442_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37442	Sample	BC37442	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB30	COI	45	BC37442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB30	ATGAACAGTCTATCCTCCTTTAG	151.77097
BC37442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89074	WGS84	BC37442	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37442_Msal_Bot2012_COI	NEWB30	1	BC37442_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37442	Sample	BC37442	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB30	COI	45	BC37442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB30	GYTAGTTCCRATGATGTTAGCTG	151.77097
BC37444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89102	WGS84	BC37444	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37444_Aamu_Bax2006_COI	NEWB28	1	BC37444_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37444	Sample	BC37444	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB28	COI	45	BC37444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB28	GCACAACCGGGATCTTTACTTC	151.76518
BC37444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89102	WGS84	BC37444	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37444_Pcan_Bot2011_IGS	NEWB28	1	BC37444_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37444	Sample	BC37444	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB28	IGS	45	BC37444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB28	CGTAATCCTCAGTACTGGCTA	151.76518
BC37444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89102	WGS84	BC37444	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37444_Sspa_Oph2007_COI	NEWB28	1	BC37444_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37444	Sample	BC37444	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB28	COI	45	BC37444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB28	GAAGGCCTTCCGATGATC	151.76518
BC37444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89102	WGS84	BC37444	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37444_Upin_Bot2011_COI	NEWB28	1	BC37444_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37444	Sample	BC37444	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB28	COI	45	BC37444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB28	CTTTAATTACAGCGTTTTTATTGTTGT	151.76518
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37445_Asen_Wil2025_COI	NEWB27	1	BC37445_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	GTTGTTGTTACAACACATGCTCTTTTG	151.77239
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.277	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37445_Mgig_Bot2012_COI	NEWB27	1	BC37445_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	TCTTATTCGTTGGAGACTTTATAACCCT	151.77239
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37446_Cmae_Bot2011_COI	NEWB26	1	BC37446_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	ATGAACAGTCTATCCTCCTTTAG	151.77011
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37446_Msal_Bot2012_COI	NEWB26	1	BC37446_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	GYTAGTTCCRATGATGTTAGCTG	151.77011
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37448_Aamu_Bax2006_COI	NEWB24	1	BC37448_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	GCACAACCGGGATCTTTACTTC	151.77132
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37448_Pcan_Bot2011_IGS	NEWB24	1	BC37448_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB24	IGS	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	CGTAATCCTCAGTACTGGCTA	151.77132
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37448_Sspa_Oph2007_COI	NEWB24	1	BC37448_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	GAAGGCCTTCCGATGATC	151.77132
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37448_Upin_Bot2011_COI	NEWB24	1	BC37448_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	CTTTAATTACAGCGTTTTTATTGTTGT	151.77132
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37448_Vgib_Wil2025_COI	NEWB24	1	BC37448_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	CCCCTCTTTACAAGTTCTTTAGGGT	151.77132
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37449_Asen_Wil2025_COI	NEWB23	1	BC37449_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	GTTGTTGTTACAACACATGCTCTTTTG	151.76928
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.272	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37449_Mgig_Bot2012_COI	NEWB23	1	BC37449_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	TCTTATTCGTTGGAGACTTTATAACCCT	151.76928
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37451_Aamu_Bax2006_COI	NEWB21	1	BC37451_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	GCACAACCGGGATCTTTACTTC	151.76998
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37451_Pcan_Bot2011_IGS	NEWB21	1	BC37451_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB21	IGS	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	CGTAATCCTCAGTACTGGCTA	151.76998
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37451_Sspa_Oph2007_COI	NEWB21	1	BC37451_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	GAAGGCCTTCCGATGATC	151.76998
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37451_Upin_Bot2011_COI	NEWB21	1	BC37451_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	CTTTAATTACAGCGTTTTTATTGTTGT	151.76998
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37451_Vgib_Wil2025_COI	NEWB21	1	BC37451_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	CCCCTCTTTACAAGTTCTTTAGGGT	151.76998
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37452_Asen_Wil2025_COI	NEWB20	1	BC37452_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	GTTGTTGTTACAACACATGCTCTTTTG	151.77454
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.042	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37452_Mgig_Bot2012_COI	NEWB20	1	BC37452_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	TCTTATTCGTTGGAGACTTTATAACCCT	151.77454
BC37453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91353	WGS84	BC37453	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37453_Cmae_Bot2011_COI	NEWB19	1	BC37453_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37453	Sample	BC37453	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB19	COI	45	BC37453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB19	ATGAACAGTCTATCCTCCTTTAG	151.77278
BC37453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91353	WGS84	BC37453	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37453_Msal_Bot2012_COI	NEWB19	1	BC37453_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37453	Sample	BC37453	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB19	COI	45	BC37453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB19	GYTAGTTCCRATGATGTTAGCTG	151.77278
BC37455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91641	WGS84	BC37455	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37455_Aamu_Bax2006_COI	NEWB17	1	BC37455_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37455	Sample	BC37455	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB17	COI	45	BC37455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB17	GCACAACCGGGATCTTTACTTC	151.77645
BC37455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91641	WGS84	BC37455	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37455_Pcan_Bot2011_IGS	NEWB17	1	BC37455_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37455	Sample	BC37455	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB17	IGS	45	BC37455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB17	CGTAATCCTCAGTACTGGCTA	151.77645
BC37455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91641	WGS84	BC37455	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37455_Sspa_Oph2007_COI	NEWB17	1	BC37455_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37455	Sample	BC37455	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB17	COI	45	BC37455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB17	GAAGGCCTTCCGATGATC	151.77645
BC37455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91641	WGS84	BC37455	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37455_Upin_Bot2011_COI	NEWB17	1	BC37455_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37455	Sample	BC37455	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB17	COI	45	BC37455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB17	CTTTAATTACAGCGTTTTTATTGTTGT	151.77645
BC37456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91743	WGS84	BC37456	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37456_Mgig_Bot2012_COI	NEWB16	1	BC37456_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37456	Sample	BC37456	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB16	COI	45	BC37456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB16	TCTTATTCGTTGGAGACTTTATAACCCT	151.77377
BC37457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91821	WGS84	BC37457	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37457_Cmae_Bot2011_COI	NEWB15	1	BC37457_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37457	Sample	BC37457	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB15	COI	45	BC37457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB15	ATGAACAGTCTATCCTCCTTTAG	151.77566
BC37457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91821	WGS84	BC37457	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37457_Msal_Bot2012_COI	NEWB15	1	BC37457_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37457	Sample	BC37457	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB15	COI	45	BC37457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB15	GYTAGTTCCRATGATGTTAGCTG	151.77566
BC37459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89868	WGS84	BC37459	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37459_Aamu_Bax2006_COI	NEWB13	1	BC37459_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37459	Sample	BC37459	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB13	COI	45	BC37459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB13	GCACAACCGGGATCTTTACTTC	151.77045
BC37459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89868	WGS84	BC37459	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37459_Pcan_Bot2011_IGS	NEWB13	1	BC37459_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37459	Sample	BC37459	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB13	IGS	45	BC37459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB13	CGTAATCCTCAGTACTGGCTA	151.77045
BC37459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89868	WGS84	BC37459	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37459_Sspa_Oph2007_COI	NEWB13	1	BC37459_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37459	Sample	BC37459	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB13	COI	45	BC37459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB13	GAAGGCCTTCCGATGATC	151.77045
BC37459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89868	WGS84	BC37459	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37459_Upin_Bot2011_COI	NEWB13	1	BC37459_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37459	Sample	BC37459	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB13	COI	45	BC37459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB13	CTTTAATTACAGCGTTTTTATTGTTGT	151.77045
BC37460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90041	WGS84	BC37460	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37460_Cmae_Bot2011_COI	NEWB12	1	BC37460_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37460	Sample	BC37460	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB12	COI	45	BC37460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB12	ATGAACAGTCTATCCTCCTTTAG	151.77233
BC37460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90041	WGS84	BC37460	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37460_Msal_Bot2012_COI	NEWB12	1	BC37460_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37460	Sample	BC37460	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB12	COI	45	BC37460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB12	GYTAGTTCCRATGATGTTAGCTG	151.77233
BC37462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90186	WGS84	BC37462	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37462_Aamu_Bax2006_COI	NEWB10	1	BC37462_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37462	Sample	BC37462	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB10	COI	45	BC37462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB10	GCACAACCGGGATCTTTACTTC	151.77113
BC37462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90186	WGS84	BC37462	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37462_Pcan_Bot2011_IGS	NEWB10	1	BC37462_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37462	Sample	BC37462	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB10	IGS	45	BC37462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB10	CGTAATCCTCAGTACTGGCTA	151.77113
BC37462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90186	WGS84	BC37462	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37462_Sspa_Oph2007_COI	NEWB10	1	BC37462_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37462	Sample	BC37462	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB10	COI	45	BC37462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB10	GAAGGCCTTCCGATGATC	151.77113
BC37462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90186	WGS84	BC37462	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37462_Upin_Bot2011_COI	NEWB10	1	BC37462_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37462	Sample	BC37462	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB10	COI	45	BC37462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB10	CTTTAATTACAGCGTTTTTATTGTTGT	151.77113
BC37463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90467	WGS84	BC37463	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37463_Mgig_Bot2012_COI	NEWB9	1	BC37463_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37463	Sample	BC37463	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB9	COI	45	BC37463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB9	TCTTATTCGTTGGAGACTTTATAACCCT	151.77235
BC37464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90649	WGS84	BC37464	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37464_Cmae_Bot2011_COI	NEWB8	1	BC37464_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37464	Sample	BC37464	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB8	COI	45	BC37464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB8	ATGAACAGTCTATCCTCCTTTAG	151.77151
BC37464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90649	WGS84	BC37464	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37464_Msal_Bot2012_COI	NEWB8	1	BC37464_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37464	Sample	BC37464	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB8	COI	45	BC37464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB8	GYTAGTTCCRATGATGTTAGCTG	151.77151
BC37466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92197	WGS84	BC37466	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37466_Aamu_Bax2006_COI	NEWB6	1	BC37466_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37466	Sample	BC37466	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB6	COI	45	BC37466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB6	GCACAACCGGGATCTTTACTTC	151.77168
BC37466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92197	WGS84	BC37466	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37466_Pcan_Bot2011_IGS	NEWB6	1	BC37466_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37466	Sample	BC37466	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB6	IGS	45	BC37466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB6	CGTAATCCTCAGTACTGGCTA	151.77168
BC37466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92197	WGS84	BC37466	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37466_Sspa_Oph2007_COI	NEWB6	1	BC37466_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37466	Sample	BC37466	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB6	COI	45	BC37466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB6	GAAGGCCTTCCGATGATC	151.77168
BC37466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92197	WGS84	BC37466	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37466_Upin_Bot2011_COI	NEWB6	1	BC37466_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37466	Sample	BC37466	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB6	COI	45	BC37466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB6	CTTTAATTACAGCGTTTTTATTGTTGT	151.77168
BC37467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.9216	WGS84	BC37467	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.428	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37467_Mgig_Bot2012_COI	NEWB5	1	BC37467_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37467	Sample	BC37467	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB5	COI	45	BC37467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB5	TCTTATTCGTTGGAGACTTTATAACCCT	151.76876
BC37468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92383	WGS84	BC37468	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37468_Cmae_Bot2011_COI	NEWB4	1	BC37468_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37468	Sample	BC37468	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB4	COI	45	BC37468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB4	ATGAACAGTCTATCCTCCTTTAG	151.77345
BC37468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92383	WGS84	BC37468	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37468_Msal_Bot2012_COI	NEWB4	1	BC37468_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37468	Sample	BC37468	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB4	COI	45	BC37468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB4	GYTAGTTCCRATGATGTTAGCTG	151.77345
BC37470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92384	WGS84	BC37470	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.19	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37470_Mgig_Bot2012_COI	NEWB2	1	BC37470_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37470	Sample	BC37470	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB2	COI	45	BC37470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB2	TCTTATTCGTTGGAGACTTTATAACCCT	151.76765
BC37471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92394	WGS84	BC37471	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37471_Cmae_Bot2011_COI	NEWB1	1	BC37471_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37471	Sample	BC37471	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB1	COI	45	BC37471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB1	ATGAACAGTCTATCCTCCTTTAG	151.76439
BC37471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92394	WGS84	BC37471	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37471_Msal_Bot2012_COI	NEWB1	1	BC37471_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37471	Sample	BC37471	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB1	COI	45	BC37471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB1	GYTAGTTCCRATGATGTTAGCTG	151.76439
BC37473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00212	WGS84	BC37473	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37473_Aamu_Bax2006_COI	BOTB2	1	BC37473_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37473	Sample	BC37473	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB2	COI	45	BC37473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB2	GCACAACCGGGATCTTTACTTC	151.21116
BC37473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00212	WGS84	BC37473	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37473_Pcan_Bot2011_IGS	BOTB2	1	BC37473_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37473	Sample	BC37473	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB2	IGS	45	BC37473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB2	CGTAATCCTCAGTACTGGCTA	151.21116
BC37473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00212	WGS84	BC37473	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37473_Sspa_Oph2007_COI	BOTB2	1	BC37473_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37473	Sample	BC37473	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB2	COI	45	BC37473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB2	GAAGGCCTTCCGATGATC	151.21116
BC37473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00212	WGS84	BC37473	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37473_Upin_Bot2011_COI	BOTB2	1	BC37473_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37473	Sample	BC37473	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB2	COI	45	BC37473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB2	CTTTAATTACAGCGTTTTTATTGTTGT	151.21116
BC37474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00023	WGS84	BC37474	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37474_Mgig_Bot2012_COI	BOTB3	1	BC37474_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37474	Sample	BC37474	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB3	COI	45	BC37474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB3	TCTTATTCGTTGGAGACTTTATAACCCT	151.20815
BC37475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99948	WGS84	BC37475	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37475_Cmae_Bot2011_COI	BOTB4	1	BC37475_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37475	Sample	BC37475	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB4	COI	45	BC37475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB4	ATGAACAGTCTATCCTCCTTTAG	151.21169
BC37475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99948	WGS84	BC37475	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37475_Msal_Bot2012_COI	BOTB4	1	BC37475_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37475	Sample	BC37475	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB4	COI	45	BC37475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB4	GYTAGTTCCRATGATGTTAGCTG	151.21169
BC37477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45332	WGS84	BC37477	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37477_Aamu_Bax2006_COI	KEMB35	1	BC37477_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37477	Sample	BC37477	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB35	COI	45	BC37477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB35	GCACAACCGGGATCTTTACTTC	150.89541
BC37477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45332	WGS84	BC37477	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37477_Pcan_Bot2011_IGS	KEMB35	1	BC37477_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37477	Sample	BC37477	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB35	IGS	45	BC37477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB35	CGTAATCCTCAGTACTGGCTA	150.89541
BC37477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45332	WGS84	BC37477	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37477_Sspa_Oph2007_COI	KEMB35	1	BC37477_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37477	Sample	BC37477	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB35	COI	45	BC37477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB35	GAAGGCCTTCCGATGATC	150.89541
BC37477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45332	WGS84	BC37477	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37477_Upin_Bot2011_COI	KEMB35	1	BC37477_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37477	Sample	BC37477	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB35	COI	45	BC37477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB35	CTTTAATTACAGCGTTTTTATTGTTGT	150.89541
BC37478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45463	WGS84	BC37478	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.216	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37478_Mgig_Bot2012_COI	KEMB34	1	BC37478_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37478	Sample	BC37478	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB34	COI	45	BC37478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB34	TCTTATTCGTTGGAGACTTTATAACCCT	150.89491
BC37479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45485	WGS84	BC37479	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37479_Cmae_Bot2011_COI	KEMB33	1	BC37479_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37479	Sample	BC37479	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB33	COI	45	BC37479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB33	ATGAACAGTCTATCCTCCTTTAG	150.8876
BC37479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45485	WGS84	BC37479	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37479_Msal_Bot2012_COI	KEMB33	1	BC37479_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37479	Sample	BC37479	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB33	COI	45	BC37479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB33	GYTAGTTCCRATGATGTTAGCTG	150.8876
BC37480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45578	WGS84	BC37480	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37480_Aamu_Bax2006_COI	KEMB32	1	BC37480_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37480	Sample	BC37480	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB32	COI	45	BC37480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB32	GCACAACCGGGATCTTTACTTC	150.89543
BC37480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45578	WGS84	BC37480	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37480_Pcan_Bot2011_IGS	KEMB32	1	BC37480_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37480	Sample	BC37480	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB32	IGS	45	BC37480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB32	CGTAATCCTCAGTACTGGCTA	150.89543
BC37480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45578	WGS84	BC37480	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37480_Sspa_Oph2007_COI	KEMB32	1	BC37480_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37480	Sample	BC37480	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB32	COI	45	BC37480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB32	GAAGGCCTTCCGATGATC	150.89543
BC37480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45578	WGS84	BC37480	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37480_Upin_Bot2011_COI	KEMB32	1	BC37480_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37480	Sample	BC37480	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB32	COI	45	BC37480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB32	CTTTAATTACAGCGTTTTTATTGTTGT	150.89543
BC37481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45662	WGS84	BC37481	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.4	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37481_Mgig_Bot2012_COI	KEMB31	1	BC37481_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37481	Sample	BC37481	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB31	COI	45	BC37481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB31	TCTTATTCGTTGGAGACTTTATAACCCT	150.89192
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37482_Cmae_Bot2011_COI	KEMB30	1	BC37482_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	ATGAACAGTCTATCCTCCTTTAG	150.88835
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37482_Msal_Bot2012_COI	KEMB30	1	BC37482_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	GYTAGTTCCRATGATGTTAGCTG	150.88835
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37484_Aamu_Bax2006_COI	KEMB28	1	BC37484_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	GCACAACCGGGATCTTTACTTC	150.89275
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37484_Pcan_Bot2011_IGS	KEMB28	1	BC37484_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB28	IGS	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	CGTAATCCTCAGTACTGGCTA	150.89275
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37484_Sspa_Oph2007_COI	KEMB28	1	BC37484_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	GAAGGCCTTCCGATGATC	150.89275
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37484_Upin_Bot2011_COI	KEMB28	1	BC37484_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	CTTTAATTACAGCGTTTTTATTGTTGT	150.89275
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37484_Vgib_Wil2025_COI	KEMB28	1	BC37484_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	CCCCTCTTTACAAGTTCTTTAGGGT	150.89275
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37485_Asen_Wil2025_COI	KEMB27	1	BC37485_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	GTTGTTGTTACAACACATGCTCTTTTG	150.89094
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.757	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37485_Mgig_Bot2012_COI	KEMB27	1	BC37485_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	TCTTATTCGTTGGAGACTTTATAACCCT	150.89094
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37486_Cmae_Bot2011_COI	KEMB26	1	BC37486_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	ATGAACAGTCTATCCTCCTTTAG	150.89653
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37486_Msal_Bot2012_COI	KEMB26	1	BC37486_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	GYTAGTTCCRATGATGTTAGCTG	150.89653
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37488_Aamu_Bax2006_COI	KEMB24	1	BC37488_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	GCACAACCGGGATCTTTACTTC	150.89227
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37488_Pcan_Bot2011_IGS	KEMB24	1	BC37488_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB24	IGS	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	CGTAATCCTCAGTACTGGCTA	150.89227
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37488_Sspa_Oph2007_COI	KEMB24	1	BC37488_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	GAAGGCCTTCCGATGATC	150.89227
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37488_Upin_Bot2011_COI	KEMB24	1	BC37488_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	CTTTAATTACAGCGTTTTTATTGTTGT	150.89227
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37488_Vgib_Wil2025_COI	KEMB24	1	BC37488_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	CCCCTCTTTACAAGTTCTTTAGGGT	150.89227
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37489_Asen_Wil2025_COI	KEMB23	1	BC37489_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	GTTGTTGTTACAACACATGCTCTTTTG	150.89793
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37489_Mgig_Bot2012_COI	KEMB23	1	BC37489_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	TCTTATTCGTTGGAGACTTTATAACCCT	150.89793
BC37491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46158	WGS84	BC37491	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37491_Aamu_Bax2006_COI	KEMB21	1	BC37491_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37491	Sample	BC37491	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB21	COI	45	BC37491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB21	GCACAACCGGGATCTTTACTTC	150.89381
BC37491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46158	WGS84	BC37491	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37491_Pcan_Bot2011_IGS	KEMB21	1	BC37491_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37491	Sample	BC37491	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB21	IGS	45	BC37491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB21	CGTAATCCTCAGTACTGGCTA	150.89381
BC37491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46158	WGS84	BC37491	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37491_Sspa_Oph2007_COI	KEMB21	1	BC37491_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37491	Sample	BC37491	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB21	COI	45	BC37491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB21	GAAGGCCTTCCGATGATC	150.89381
BC37491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46158	WGS84	BC37491	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37491_Upin_Bot2011_COI	KEMB21	1	BC37491_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37491	Sample	BC37491	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB21	COI	45	BC37491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB21	CTTTAATTACAGCGTTTTTATTGTTGT	150.89381
BC37492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46283	WGS84	BC37492	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37492_Mgig_Bot2012_COI	KEMB20	1	BC37492_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37492	Sample	BC37492	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB20	COI	45	BC37492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB20	TCTTATTCGTTGGAGACTTTATAACCCT	150.89739
BC37493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46383	WGS84	BC37493	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37493_Cmae_Bot2011_COI	KEMB19	1	BC37493_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37493	Sample	BC37493	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB19	COI	45	BC37493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB19	ATGAACAGTCTATCCTCCTTTAG	150.90512
BC37493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46383	WGS84	BC37493	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37493_Msal_Bot2012_COI	KEMB19	1	BC37493_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37493	Sample	BC37493	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB19	COI	45	BC37493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB19	GYTAGTTCCRATGATGTTAGCTG	150.90512
BC37495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46364	WGS84	BC37495	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37495_Aamu_Bax2006_COI	KEMB17	1	BC37495_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37495	Sample	BC37495	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB17	COI	45	BC37495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB17	GCACAACCGGGATCTTTACTTC	150.90002
BC37495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46364	WGS84	BC37495	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37495_Pcan_Bot2011_IGS	KEMB17	1	BC37495_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37495	Sample	BC37495	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB17	IGS	45	BC37495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB17	CGTAATCCTCAGTACTGGCTA	150.90002
BC37495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46364	WGS84	BC37495	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37495_Sspa_Oph2007_COI	KEMB17	1	BC37495_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37495	Sample	BC37495	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB17	COI	45	BC37495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB17	GAAGGCCTTCCGATGATC	150.90002
BC37495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46364	WGS84	BC37495	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37495_Upin_Bot2011_COI	KEMB17	1	BC37495_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37495	Sample	BC37495	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB17	COI	45	BC37495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB17	CTTTAATTACAGCGTTTTTATTGTTGT	150.90002
BC37496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46441	WGS84	BC37496	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37496_Mgig_Bot2012_COI	KEMB16	1	BC37496_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37496	Sample	BC37496	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB16	COI	45	BC37496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB16	TCTTATTCGTTGGAGACTTTATAACCCT	150.9084
BC37497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46504	WGS84	BC37497	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37497_Cmae_Bot2011_COI	KEMB15	1	BC37497_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37497	Sample	BC37497	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB15	COI	45	BC37497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB15	ATGAACAGTCTATCCTCCTTTAG	150.90708
BC37497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46504	WGS84	BC37497	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37497_Msal_Bot2012_COI	KEMB15	1	BC37497_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37497	Sample	BC37497	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB15	COI	45	BC37497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB15	GYTAGTTCCRATGATGTTAGCTG	150.90708
BC37499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46502	WGS84	BC37499	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37499_Aamu_Bax2006_COI	KEMB13	1	BC37499_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37499	Sample	BC37499	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB13	COI	45	BC37499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB13	GCACAACCGGGATCTTTACTTC	150.90137
BC37499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46502	WGS84	BC37499	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37499_Pcan_Bot2011_IGS	KEMB13	1	BC37499_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37499	Sample	BC37499	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB13	IGS	45	BC37499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB13	CGTAATCCTCAGTACTGGCTA	150.90137
BC37499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46502	WGS84	BC37499	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37499_Sspa_Oph2007_COI	KEMB13	1	BC37499_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37499	Sample	BC37499	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB13	COI	45	BC37499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB13	GAAGGCCTTCCGATGATC	150.90137
BC37499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46502	WGS84	BC37499	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37499_Upin_Bot2011_COI	KEMB13	1	BC37499_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37499	Sample	BC37499	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB13	COI	45	BC37499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB13	CTTTAATTACAGCGTTTTTATTGTTGT	150.90137
BC37500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46622	WGS84	BC37500	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37500_Mgig_Bot2012_COI	KEMB12	1	BC37500_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37500	Sample	BC37500	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB12	COI	45	BC37500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB12	TCTTATTCGTTGGAGACTTTATAACCCT	150.90847
BC37501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46709	WGS84	BC37501	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37501_Cmae_Bot2011_COI	KEMB11	1	BC37501_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37501	Sample	BC37501	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB11	COI	45	BC37501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB11	ATGAACAGTCTATCCTCCTTTAG	150.90581
BC37501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46709	WGS84	BC37501	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37501_Msal_Bot2012_COI	KEMB11	1	BC37501_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37501	Sample	BC37501	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB11	COI	45	BC37501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB11	GYTAGTTCCRATGATGTTAGCTG	150.90581
BC37503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46663	WGS84	BC37503	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37503_Aamu_Bax2006_COI	KEMB9	1	BC37503_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37503	Sample	BC37503	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB9	COI	45	BC37503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB9	GCACAACCGGGATCTTTACTTC	150.90134
BC37503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46663	WGS84	BC37503	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37503_Pcan_Bot2011_IGS	KEMB9	1	BC37503_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37503	Sample	BC37503	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB9	IGS	45	BC37503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB9	CGTAATCCTCAGTACTGGCTA	150.90134
BC37503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46663	WGS84	BC37503	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37503_Sspa_Oph2007_COI	KEMB9	1	BC37503_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37503	Sample	BC37503	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB9	COI	45	BC37503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB9	GAAGGCCTTCCGATGATC	150.90134
BC37503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46663	WGS84	BC37503	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37503_Upin_Bot2011_COI	KEMB9	1	BC37503_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37503	Sample	BC37503	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB9	COI	45	BC37503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB9	CTTTAATTACAGCGTTTTTATTGTTGT	150.90134
BC37504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46864	WGS84	BC37504	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37504_Mgig_Bot2012_COI	KEMB8	1	BC37504_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37504	Sample	BC37504	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB8	COI	45	BC37504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB8	TCTTATTCGTTGGAGACTTTATAACCCT	150.90694
BC37505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46901	WGS84	BC37505	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37505_Cmae_Bot2011_COI	KEMB7	1	BC37505_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37505	Sample	BC37505	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB7	COI	45	BC37505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB7	ATGAACAGTCTATCCTCCTTTAG	150.90504
BC37505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46901	WGS84	BC37505	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37505_Msal_Bot2012_COI	KEMB7	1	BC37505_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37505	Sample	BC37505	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB7	COI	45	BC37505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB7	GYTAGTTCCRATGATGTTAGCTG	150.90504
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63715_Cmae_Bot2011_COI	FREB30	1	BC63715_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	ATGAACAGTCTATCCTCCTTTAG	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63715_Msal_Bot2012_COI	FREB30	1	BC63715_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Msal_Bot2012_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	GYTAGTTCCRATGATGTTAGCTG	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63715_Pvir_Dia2013_COI	FREB30	1	BC63715_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	CTTAGTGGCATTAATTCGDAATCC	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63715_Rhar_Sim2018_COI	FREB30	1	BC63715_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	CTGCTGCTATTGCTCATG	115.74996
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63717_Aamu_Bax2006_COI	FREB20	1	BC63717_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	GCACAACCGGGATCTTTACTTC	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63717_Pcan_Bot2011_IGS	FREB20	1	BC63717_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB20	IGS	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	CGTAATCCTCAGTACTGGCTA	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63717_Pper_Dia2013_COI	FREB20	1	BC63717_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Pper_Dia2013_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	CTTAGTGGCATTAATTCGDAATCC	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63717_Sspa_Oph2007_COI	FREB20	1	BC63717_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	GAAGGCCTTCCGATGATC	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63717_Upin_Bot2011_COI	FREB20	1	BC63717_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Upin_Bot2011_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	CTTTAATTACAGCGTTTTTATTGTTGT	115.74722
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63718_Cjap_Sim2018_COI	FREB15	1	BC63718_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63718_Mgig_Bot2012_COI	FREB15	1	BC63718_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	TCTTATTCGTTGGAGACTTTATAACCCT	115.74405
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63719_Cmae_Bot2011_COI	FREB10	1	BC63719_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	ATGAACAGTCTATCCTCCTTTAG	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63719_Msal_Bot2012_COI	FREB10	1	BC63719_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Msal_Bot2012_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	GYTAGTTCCRATGATGTTAGCTG	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63719_Pvir_Dia2013_COI	FREB10	1	BC63719_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	CTTAGTGGCATTAATTCGDAATCC	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63719_Rhar_Sim2018_COI	FREB10	1	BC63719_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	CTGCTGCTATTGCTCATG	115.74163
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63720_Aamu_Bax2006_COI	FREB5	1	BC63720_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	GCACAACCGGGATCTTTACTTC	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63720_Pcan_Bot2011_IGS	FREB5	1	BC63720_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB5	IGS	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	CGTAATCCTCAGTACTGGCTA	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63720_Pper_Dia2013_COI	FREB5	1	BC63720_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Pper_Dia2013_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	CTTAGTGGCATTAATTCGDAATCC	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63720_Sspa_Oph2007_COI	FREB5	1	BC63720_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	GAAGGCCTTCCGATGATC	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63720_Upin_Bot2011_COI	FREB5	1	BC63720_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Upin_Bot2011_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	CTTTAATTACAGCGTTTTTATTGTTGT	115.74062
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63721_Cjap_Sim2018_COI	FREB33	1	BC63721_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63721_Mgig_Bot2012_COI	FREB33	1	BC63721_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	TCTTATTCGTTGGAGACTTTATAACCCT	115.75105
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63722_Cmae_Bot2011_COI	FREB32	1	BC63722_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	ATGAACAGTCTATCCTCCTTTAG	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63722_Msal_Bot2012_COI	FREB32	1	BC63722_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Msal_Bot2012_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	GYTAGTTCCRATGATGTTAGCTG	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63722_Pvir_Dia2013_COI	FREB32	1	BC63722_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	CTTAGTGGCATTAATTCGDAATCC	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63722_Rhar_Sim2018_COI	FREB32	1	BC63722_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	CTGCTGCTATTGCTCATG	115.7509
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63724_Aamu_Bax2006_COI	FREB29	1	BC63724_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	GCACAACCGGGATCTTTACTTC	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63724_Pcan_Bot2011_IGS	FREB29	1	BC63724_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB29	IGS	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	CGTAATCCTCAGTACTGGCTA	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63724_Pper_Dia2013_COI	FREB29	1	BC63724_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Pper_Dia2013_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	CTTAGTGGCATTAATTCGDAATCC	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63724_Sspa_Oph2007_COI	FREB29	1	BC63724_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	GAAGGCCTTCCGATGATC	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63724_Upin_Bot2011_COI	FREB29	1	BC63724_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Upin_Bot2011_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	CTTTAATTACAGCGTTTTTATTGTTGT	115.7501
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63725_Cjap_Sim2018_COI	FREB28	1	BC63725_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63725_Mgig_Bot2012_COI	FREB28	1	BC63725_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	TCTTATTCGTTGGAGACTTTATAACCCT	115.74897
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63726_Cmae_Bot2011_COI	FREB27	1	BC63726_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	ATGAACAGTCTATCCTCCTTTAG	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63726_Msal_Bot2012_COI	FREB27	1	BC63726_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Msal_Bot2012_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	GYTAGTTCCRATGATGTTAGCTG	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63726_Pvir_Dia2013_COI	FREB27	1	BC63726_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	CTTAGTGGCATTAATTCGDAATCC	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63726_Rhar_Sim2018_COI	FREB27	1	BC63726_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	CTGCTGCTATTGCTCATG	115.74747
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63728_Aamu_Bax2006_COI	FREB24	1	BC63728_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	GCACAACCGGGATCTTTACTTC	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63728_Pcan_Bot2011_IGS	FREB24	1	BC63728_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB24	IGS	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	CGTAATCCTCAGTACTGGCTA	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63728_Pper_Dia2013_COI	FREB24	1	BC63728_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Pper_Dia2013_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	CTTAGTGGCATTAATTCGDAATCC	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63728_Sspa_Oph2007_COI	FREB24	1	BC63728_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	GAAGGCCTTCCGATGATC	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63728_Upin_Bot2011_COI	FREB24	1	BC63728_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Upin_Bot2011_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	CTTTAATTACAGCGTTTTTATTGTTGT	115.7464
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63729_Cjap_Sim2018_COI	FREB23	1	BC63729_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63729_Mgig_Bot2012_COI	FREB23	1	BC63729_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	TCTTATTCGTTGGAGACTTTATAACCCT	115.74823
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63731_Aamu_Bax2006_COI	FREB21	1	BC63731_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	GCACAACCGGGATCTTTACTTC	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63731_Pcan_Bot2011_IGS	FREB21	1	BC63731_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB21	IGS	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	CGTAATCCTCAGTACTGGCTA	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63731_Pper_Dia2013_COI	FREB21	1	BC63731_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Pper_Dia2013_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	CTTAGTGGCATTAATTCGDAATCC	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63731_Sspa_Oph2007_COI	FREB21	1	BC63731_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	GAAGGCCTTCCGATGATC	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63731_Upin_Bot2011_COI	FREB21	1	BC63731_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Upin_Bot2011_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	CTTTAATTACAGCGTTTTTATTGTTGT	115.74527
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04564	WGS84	BC63732	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	25.231	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63732_Asen_Wil2025_COI	FREB19	1	BC63732_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Present	Sample	Asen_Wil2025_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	GTTGTTGTTACAACACATGCTCTTTTG	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63732_Cjap_Sim2018_COI	FREB19	1	BC63732_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63732_Mgig_Bot2012_COI	FREB19	1	BC63732_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	TCTTATTCGTTGGAGACTTTATAACCCT	115.74577
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63733_Cmae_Bot2011_COI	FREB18	1	BC63733_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	ATGAACAGTCTATCCTCCTTTAG	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63733_Msal_Bot2012_COI	FREB18	1	BC63733_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Msal_Bot2012_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	GYTAGTTCCRATGATGTTAGCTG	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63733_Pvir_Dia2013_COI	FREB18	1	BC63733_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	CTTAGTGGCATTAATTCGDAATCC	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63733_Rhar_Sim2018_COI	FREB18	1	BC63733_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	CTGCTGCTATTGCTCATG	115.74461
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63735_Aamu_Bax2006_COI	FREB16	1	BC63735_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	GCACAACCGGGATCTTTACTTC	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63735_Pcan_Bot2011_IGS	FREB16	1	BC63735_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB16	IGS	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	CGTAATCCTCAGTACTGGCTA	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63735_Pper_Dia2013_COI	FREB16	1	BC63735_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Pper_Dia2013_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	CTTAGTGGCATTAATTCGDAATCC	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63735_Sspa_Oph2007_COI	FREB16	1	BC63735_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	GAAGGCCTTCCGATGATC	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63735_Upin_Bot2011_COI	FREB16	1	BC63735_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Upin_Bot2011_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	CTTTAATTACAGCGTTTTTATTGTTGT	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04642	WGS84	BC63735	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63735_Vgib_Wil2025_COI	FREB16	1	BC63735_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	CCCCTCTTTACAAGTTCTTTAGGGT	115.7444
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04787	WGS84	BC63736	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	23.71	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63736_Asen_Wil2025_COI	FREB14	1	BC63736_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Present	Sample	Asen_Wil2025_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	GTTGTTGTTACAACACATGCTCTTTTG	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63736_Cjap_Sim2018_COI	FREB14	1	BC63736_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63736_Mgig_Bot2012_COI	FREB14	1	BC63736_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	TCTTATTCGTTGGAGACTTTATAACCCT	115.74513
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63737_Cmae_Bot2011_COI	FREB13	1	BC63737_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	ATGAACAGTCTATCCTCCTTTAG	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63737_Msal_Bot2012_COI	FREB13	1	BC63737_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Msal_Bot2012_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	GYTAGTTCCRATGATGTTAGCTG	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63737_Pvir_Dia2013_COI	FREB13	1	BC63737_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	CTTAGTGGCATTAATTCGDAATCC	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63737_Rhar_Sim2018_COI	FREB13	1	BC63737_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	CTGCTGCTATTGCTCATG	115.74378
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63739_Aamu_Bax2006_COI	FREB11	1	BC63739_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	GCACAACCGGGATCTTTACTTC	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63739_Pcan_Bot2011_IGS	FREB11	1	BC63739_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB11	IGS	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	CGTAATCCTCAGTACTGGCTA	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63739_Pper_Dia2013_COI	FREB11	1	BC63739_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Pper_Dia2013_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	CTTAGTGGCATTAATTCGDAATCC	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63739_Sspa_Oph2007_COI	FREB11	1	BC63739_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	GAAGGCCTTCCGATGATC	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63739_Upin_Bot2011_COI	FREB11	1	BC63739_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Upin_Bot2011_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	CTTTAATTACAGCGTTTTTATTGTTGT	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04898	WGS84	BC63739	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63739_Vgib_Wil2025_COI	FREB11	1	BC63739_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	CCCCTCTTTACAAGTTCTTTAGGGT	115.74423
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63740_Cmae_Bot2011_COI	FREB9	1	BC63740_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	ATGAACAGTCTATCCTCCTTTAG	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63740_Msal_Bot2012_COI	FREB9	1	BC63740_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Msal_Bot2012_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	GYTAGTTCCRATGATGTTAGCTG	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63740_Pvir_Dia2013_COI	FREB9	1	BC63740_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	CTTAGTGGCATTAATTCGDAATCC	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63740_Rhar_Sim2018_COI	FREB9	1	BC63740_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	CTGCTGCTATTGCTCATG	115.74163
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63742_Aamu_Bax2006_COI	FREB7	1	BC63742_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	GCACAACCGGGATCTTTACTTC	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63742_Pcan_Bot2011_IGS	FREB7	1	BC63742_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB7	IGS	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	CGTAATCCTCAGTACTGGCTA	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63742_Pper_Dia2013_COI	FREB7	1	BC63742_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Pper_Dia2013_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	CTTAGTGGCATTAATTCGDAATCC	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63742_Sspa_Oph2007_COI	FREB7	1	BC63742_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	GAAGGCCTTCCGATGATC	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63742_Upin_Bot2011_COI	FREB7	1	BC63742_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Upin_Bot2011_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	CTTTAATTACAGCGTTTTTATTGTTGT	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05009	WGS84	BC63742	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63742_Vgib_Wil2025_COI	FREB7	1	BC63742_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	CCCCTCTTTACAAGTTCTTTAGGGT	115.74177
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05114	WGS84	BC63743	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	23.37	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63743_Asen_Wil2025_COI	FREB6	1	BC63743_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Present	Sample	Asen_Wil2025_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	GTTGTTGTTACAACACATGCTCTTTTG	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63743_Cjap_Sim2018_COI	FREB6	1	BC63743_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63743_Mgig_Bot2012_COI	FREB6	1	BC63743_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	TCTTATTCGTTGGAGACTTTATAACCCT	115.74209
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63744_Cmae_Bot2011_COI	FREB4	1	BC63744_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	ATGAACAGTCTATCCTCCTTTAG	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63744_Msal_Bot2012_COI	FREB4	1	BC63744_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Msal_Bot2012_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	GYTAGTTCCRATGATGTTAGCTG	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63744_Pvir_Dia2013_COI	FREB4	1	BC63744_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	CTTAGTGGCATTAATTCGDAATCC	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63744_Rhar_Sim2018_COI	FREB4	1	BC63744_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	CTGCTGCTATTGCTCATG	115.74122
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63746_Aamu_Bax2006_COI	FREB2	1	BC63746_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	GCACAACCGGGATCTTTACTTC	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63746_Pcan_Bot2011_IGS	FREB2	1	BC63746_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB2	IGS	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	CGTAATCCTCAGTACTGGCTA	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63746_Pper_Dia2013_COI	FREB2	1	BC63746_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Pper_Dia2013_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	CTTAGTGGCATTAATTCGDAATCC	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63746_Sspa_Oph2007_COI	FREB2	1	BC63746_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	GAAGGCCTTCCGATGATC	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63746_Upin_Bot2011_COI	FREB2	1	BC63746_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Upin_Bot2011_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	CTTTAATTACAGCGTTTTTATTGTTGT	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05337	WGS84	BC63746	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63746_Vgib_Wil2025_COI	FREB2	1	BC63746_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	CCCCTCTTTACAAGTTCTTTAGGGT	115.73868
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05427	WGS84	BC63747	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	28.648	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63747_Asen_Wil2025_COI	FREB1	1	BC63747_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Present	Sample	Asen_Wil2025_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	GTTGTTGTTACAACACATGCTCTTTTG	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63747_Cjap_Sim2018_COI	FREB1	1	BC63747_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63747_Mgig_Bot2012_COI	FREB1	1	BC63747_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	TCTTATTCGTTGGAGACTTTATAACCCT	115.73746
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63748_Cmae_Bot2011_COI	KWIB60	1	BC63748_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	ATGAACAGTCTATCCTCCTTTAG	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63748_Msal_Bot2012_COI	KWIB60	1	BC63748_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	GYTAGTTCCRATGATGTTAGCTG	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63748_Pvir_Dia2013_COI	KWIB60	1	BC63748_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	CTTAGTGGCATTAATTCGDAATCC	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63748_Rhar_Sim2018_COI	KWIB60	1	BC63748_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	CTGCTGCTATTGCTCATG	115.77273
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63750_Cjap_Sim2018_COI	KWIB50	1	BC63750_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63750_Mgig_Bot2012_COI	KWIB50	1	BC63750_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	TCTTATTCGTTGGAGACTTTATAACCCT	115.76393
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63751_Cmae_Bot2011_COI	KWIB45	1	BC63751_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	ATGAACAGTCTATCCTCCTTTAG	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63751_Msal_Bot2012_COI	KWIB45	1	BC63751_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	GYTAGTTCCRATGATGTTAGCTG	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63751_Pvir_Dia2013_COI	KWIB45	1	BC63751_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	CTTAGTGGCATTAATTCGDAATCC	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63751_Rhar_Sim2018_COI	KWIB45	1	BC63751_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	CTGCTGCTATTGCTCATG	115.76082
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63753_Aamu_Bax2006_COI	KWIB35	1	BC63753_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	GCACAACCGGGATCTTTACTTC	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63753_Pcan_Bot2011_IGS	KWIB35	1	BC63753_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB35	IGS	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	CGTAATCCTCAGTACTGGCTA	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63753_Pper_Dia2013_COI	KWIB35	1	BC63753_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	CTTAGTGGCATTAATTCGDAATCC	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63753_Sspa_Oph2007_COI	KWIB35	1	BC63753_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	GAAGGCCTTCCGATGATC	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63753_Upin_Bot2011_COI	KWIB35	1	BC63753_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	CTTTAATTACAGCGTTTTTATTGTTGT	115.75715
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63754_Cjap_Sim2018_COI	KWIB30	1	BC63754_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63754_Mgig_Bot2012_COI	KWIB30	1	BC63754_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	TCTTATTCGTTGGAGACTTTATAACCCT	115.75607
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63755_Cmae_Bot2011_COI	KWIB25	1	BC63755_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	ATGAACAGTCTATCCTCCTTTAG	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63755_Msal_Bot2012_COI	KWIB25	1	BC63755_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	GYTAGTTCCRATGATGTTAGCTG	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63755_Pvir_Dia2013_COI	KWIB25	1	BC63755_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	CTTAGTGGCATTAATTCGDAATCC	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63755_Rhar_Sim2018_COI	KWIB25	1	BC63755_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	CTGCTGCTATTGCTCATG	115.75317
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63757_Aamu_Bax2006_COI	KWIB15	1	BC63757_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	GCACAACCGGGATCTTTACTTC	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63757_Pcan_Bot2011_IGS	KWIB15	1	BC63757_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB15	IGS	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	CGTAATCCTCAGTACTGGCTA	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63757_Pper_Dia2013_COI	KWIB15	1	BC63757_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	CTTAGTGGCATTAATTCGDAATCC	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63757_Sspa_Oph2007_COI	KWIB15	1	BC63757_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	GAAGGCCTTCCGATGATC	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63757_Upin_Bot2011_COI	KWIB15	1	BC63757_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	CTTTAATTACAGCGTTTTTATTGTTGT	115.75598
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63758_Cjap_Sim2018_COI	KWIB10	1	BC63758_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63758_Mgig_Bot2012_COI	KWIB10	1	BC63758_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	TCTTATTCGTTGGAGACTTTATAACCCT	115.74383
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63759_Cmae_Bot2011_COI	KWIB5	1	BC63759_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	ATGAACAGTCTATCCTCCTTTAG	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63759_Msal_Bot2012_COI	KWIB5	1	BC63759_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	GYTAGTTCCRATGATGTTAGCTG	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63759_Pvir_Dia2013_COI	KWIB5	1	BC63759_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	CTTAGTGGCATTAATTCGDAATCC	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63759_Rhar_Sim2018_COI	KWIB5	1	BC63759_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	CTGCTGCTATTGCTCATG	115.74466
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63760_Aamu_Bax2006_COI	KWIB59	1	BC63760_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	GCACAACCGGGATCTTTACTTC	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63760_Pcan_Bot2011_IGS	KWIB59	1	BC63760_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB59	IGS	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	CGTAATCCTCAGTACTGGCTA	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63760_Pper_Dia2013_COI	KWIB59	1	BC63760_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	CTTAGTGGCATTAATTCGDAATCC	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63760_Sspa_Oph2007_COI	KWIB59	1	BC63760_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	GAAGGCCTTCCGATGATC	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63760_Upin_Bot2011_COI	KWIB59	1	BC63760_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	CTTTAATTACAGCGTTTTTATTGTTGT	115.77164
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63761_Cjap_Sim2018_COI	KWIB58	1	BC63761_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63761_Mgig_Bot2012_COI	KWIB58	1	BC63761_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	TCTTATTCGTTGGAGACTTTATAACCCT	115.76952
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63762_Cmae_Bot2011_COI	KWIB57	1	BC63762_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	ATGAACAGTCTATCCTCCTTTAG	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63762_Msal_Bot2012_COI	KWIB57	1	BC63762_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	GYTAGTTCCRATGATGTTAGCTG	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63762_Pvir_Dia2013_COI	KWIB57	1	BC63762_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	CTTAGTGGCATTAATTCGDAATCC	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63762_Rhar_Sim2018_COI	KWIB57	1	BC63762_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	CTGCTGCTATTGCTCATG	115.7729
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63764_Aamu_Bax2006_COI	KWIB54	1	BC63764_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	GCACAACCGGGATCTTTACTTC	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63764_Pcan_Bot2011_IGS	KWIB54	1	BC63764_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB54	IGS	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	CGTAATCCTCAGTACTGGCTA	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63764_Pper_Dia2013_COI	KWIB54	1	BC63764_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	CTTAGTGGCATTAATTCGDAATCC	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63764_Sspa_Oph2007_COI	KWIB54	1	BC63764_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	GAAGGCCTTCCGATGATC	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63764_Upin_Bot2011_COI	KWIB54	1	BC63764_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	CTTTAATTACAGCGTTTTTATTGTTGT	115.77258
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63765_Cjap_Sim2018_COI	KWIB53	1	BC63765_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63765_Mgig_Bot2012_COI	KWIB53	1	BC63765_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	TCTTATTCGTTGGAGACTTTATAACCCT	115.77138
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63766_Cmae_Bot2011_COI	KWIB52	1	BC63766_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	ATGAACAGTCTATCCTCCTTTAG	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63766_Msal_Bot2012_COI	KWIB52	1	BC63766_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	GYTAGTTCCRATGATGTTAGCTG	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63766_Pvir_Dia2013_COI	KWIB52	1	BC63766_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	CTTAGTGGCATTAATTCGDAATCC	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63766_Rhar_Sim2018_COI	KWIB52	1	BC63766_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	CTGCTGCTATTGCTCATG	115.76958
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63768_Aamu_Bax2006_COI	KWIB49	1	BC63768_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	GCACAACCGGGATCTTTACTTC	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63768_Pcan_Bot2011_IGS	KWIB49	1	BC63768_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB49	IGS	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	CGTAATCCTCAGTACTGGCTA	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63768_Pper_Dia2013_COI	KWIB49	1	BC63768_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	CTTAGTGGCATTAATTCGDAATCC	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63768_Sspa_Oph2007_COI	KWIB49	1	BC63768_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	GAAGGCCTTCCGATGATC	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63768_Upin_Bot2011_COI	KWIB49	1	BC63768_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	CTTTAATTACAGCGTTTTTATTGTTGT	115.76462
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63769_Cjap_Sim2018_COI	KWIB48	1	BC63769_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63769_Mgig_Bot2012_COI	KWIB48	1	BC63769_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	TCTTATTCGTTGGAGACTTTATAACCCT	115.76106
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63771_Aamu_Bax2006_COI	KWIB46	1	BC63771_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	GCACAACCGGGATCTTTACTTC	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63771_Pcan_Bot2011_IGS	KWIB46	1	BC63771_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB46	IGS	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	CGTAATCCTCAGTACTGGCTA	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63771_Pper_Dia2013_COI	KWIB46	1	BC63771_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	CTTAGTGGCATTAATTCGDAATCC	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63771_Sspa_Oph2007_COI	KWIB46	1	BC63771_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	GAAGGCCTTCCGATGATC	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63771_Upin_Bot2011_COI	KWIB46	1	BC63771_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	CTTTAATTACAGCGTTTTTATTGTTGT	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21083	WGS84	BC63771	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63771_Vgib_Wil2025_COI	KWIB46	1	BC63771_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	CCCCTCTTTACAAGTTCTTTAGGGT	115.76322
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21242	WGS84	BC63772	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	32.521	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63772_Asen_Wil2025_COI	KWIB44	1	BC63772_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Present	Sample	Asen_Wil2025_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	GTTGTTGTTACAACACATGCTCTTTTG	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63772_Cjap_Sim2018_COI	KWIB44	1	BC63772_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63772_Mgig_Bot2012_COI	KWIB44	1	BC63772_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	TCTTATTCGTTGGAGACTTTATAACCCT	115.76168
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63773_Cmae_Bot2011_COI	KWIB43	1	BC63773_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	ATGAACAGTCTATCCTCCTTTAG	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63773_Msal_Bot2012_COI	KWIB43	1	BC63773_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	GYTAGTTCCRATGATGTTAGCTG	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63773_Pvir_Dia2013_COI	KWIB43	1	BC63773_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	CTTAGTGGCATTAATTCGDAATCC	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63773_Rhar_Sim2018_COI	KWIB43	1	BC63773_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	CTGCTGCTATTGCTCATG	115.75911
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63775_Aamu_Bax2006_COI	KWIB41	1	BC63775_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	GCACAACCGGGATCTTTACTTC	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63775_Pcan_Bot2011_IGS	KWIB41	1	BC63775_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB41	IGS	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	CGTAATCCTCAGTACTGGCTA	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63775_Pper_Dia2013_COI	KWIB41	1	BC63775_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	CTTAGTGGCATTAATTCGDAATCC	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.002	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63775_Sspa_Oph2007_COI	KWIB41	1	BC63775_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	GAAGGCCTTCCGATGATC	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63775_Upin_Bot2011_COI	KWIB41	1	BC63775_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	CTTTAATTACAGCGTTTTTATTGTTGT	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22831	WGS84	BC63775	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63775_Vgib_Wil2025_COI	KWIB41	1	BC63775_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	CCCCTCTTTACAAGTTCTTTAGGGT	115.75533
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22993	WGS84	BC63776	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.905	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63776_Asen_Wil2025_COI	KWIB39	1	BC63776_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Present	Sample	Asen_Wil2025_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	GTTGTTGTTACAACACATGCTCTTTTG	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63776_Cjap_Sim2018_COI	KWIB39	1	BC63776_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63776_Mgig_Bot2012_COI	KWIB39	1	BC63776_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	TCTTATTCGTTGGAGACTTTATAACCCT	115.75608
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63777_Cmae_Bot2011_COI	KWIB38	1	BC63777_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	ATGAACAGTCTATCCTCCTTTAG	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63777_Msal_Bot2012_COI	KWIB38	1	BC63777_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	GYTAGTTCCRATGATGTTAGCTG	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63777_Pvir_Dia2013_COI	KWIB38	1	BC63777_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	CTTAGTGGCATTAATTCGDAATCC	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63777_Rhar_Sim2018_COI	KWIB38	1	BC63777_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	CTGCTGCTATTGCTCATG	115.75292
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63779_Aamu_Bax2006_COI	KWIB36	1	BC63779_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	GCACAACCGGGATCTTTACTTC	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63779_Pcan_Bot2011_IGS	KWIB36	1	BC63779_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB36	IGS	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	CGTAATCCTCAGTACTGGCTA	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63779_Pper_Dia2013_COI	KWIB36	1	BC63779_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	CTTAGTGGCATTAATTCGDAATCC	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63779_Sspa_Oph2007_COI	KWIB36	1	BC63779_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	GAAGGCCTTCCGATGATC	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63779_Upin_Bot2011_COI	KWIB36	1	BC63779_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	CTTTAATTACAGCGTTTTTATTGTTGT	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23195	WGS84	BC63779	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63779_Vgib_Wil2025_COI	KWIB36	1	BC63779_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	CCCCTCTTTACAAGTTCTTTAGGGT	115.75378
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63780_Cmae_Bot2011_COI	KWIB34	1	BC63780_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	ATGAACAGTCTATCCTCCTTTAG	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63780_Msal_Bot2012_COI	KWIB34	1	BC63780_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	GYTAGTTCCRATGATGTTAGCTG	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63780_Pvir_Dia2013_COI	KWIB34	1	BC63780_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	CTTAGTGGCATTAATTCGDAATCC	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63780_Rhar_Sim2018_COI	KWIB34	1	BC63780_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	CTGCTGCTATTGCTCATG	115.75612
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63782_Aamu_Bax2006_COI	KWIB32	1	BC63782_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	GCACAACCGGGATCTTTACTTC	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63782_Pcan_Bot2011_IGS	KWIB32	1	BC63782_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB32	IGS	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	CGTAATCCTCAGTACTGGCTA	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63782_Pper_Dia2013_COI	KWIB32	1	BC63782_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	CTTAGTGGCATTAATTCGDAATCC	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63782_Sspa_Oph2007_COI	KWIB32	1	BC63782_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	GAAGGCCTTCCGATGATC	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63782_Upin_Bot2011_COI	KWIB32	1	BC63782_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	CTTTAATTACAGCGTTTTTATTGTTGT	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23397	WGS84	BC63782	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63782_Vgib_Wil2025_COI	KWIB32	1	BC63782_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	CCCCTCTTTACAAGTTCTTTAGGGT	115.75315
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23656	WGS84	BC63783	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63783_Asen_Wil2025_COI	KWIB31	1	BC63783_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	GTTGTTGTTACAACACATGCTCTTTTG	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63783_Cjap_Sim2018_COI	KWIB31	1	BC63783_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63783_Mgig_Bot2012_COI	KWIB31	1	BC63783_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	TCTTATTCGTTGGAGACTTTATAACCCT	115.75781
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63784_Cmae_Bot2011_COI	KWIB29	1	BC63784_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	ATGAACAGTCTATCCTCCTTTAG	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63784_Msal_Bot2012_COI	KWIB29	1	BC63784_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	GYTAGTTCCRATGATGTTAGCTG	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63784_Pvir_Dia2013_COI	KWIB29	1	BC63784_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	CTTAGTGGCATTAATTCGDAATCC	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63784_Rhar_Sim2018_COI	KWIB29	1	BC63784_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	CTGCTGCTATTGCTCATG	115.75441
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63786_Aamu_Bax2006_COI	KWIB27	1	BC63786_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	GCACAACCGGGATCTTTACTTC	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63786_Pcan_Bot2011_IGS	KWIB27	1	BC63786_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB27	IGS	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	CGTAATCCTCAGTACTGGCTA	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63786_Pper_Dia2013_COI	KWIB27	1	BC63786_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	CTTAGTGGCATTAATTCGDAATCC	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63786_Sspa_Oph2007_COI	KWIB27	1	BC63786_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	GAAGGCCTTCCGATGATC	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63786_Upin_Bot2011_COI	KWIB27	1	BC63786_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	CTTTAATTACAGCGTTTTTATTGTTGT	115.75851
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63787_Cjap_Sim2018_COI	KWIB26	1	BC63787_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63787_Mgig_Bot2012_COI	KWIB26	1	BC63787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	TCTTATTCGTTGGAGACTTTATAACCCT	115.75575
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63788_Cmae_Bot2011_COI	KWIB24	1	BC63788_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	ATGAACAGTCTATCCTCCTTTAG	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63788_Msal_Bot2012_COI	KWIB24	1	BC63788_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	GYTAGTTCCRATGATGTTAGCTG	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63788_Pvir_Dia2013_COI	KWIB24	1	BC63788_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	CTTAGTGGCATTAATTCGDAATCC	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63788_Rhar_Sim2018_COI	KWIB24	1	BC63788_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	CTGCTGCTATTGCTCATG	115.75724
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63790_Cjap_Sim2018_COI	KWIB22	1	BC63790_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63790_Mgig_Bot2012_COI	KWIB22	1	BC63790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	TCTTATTCGTTGGAGACTTTATAACCCT	115.75808
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63791_Cmae_Bot2011_COI	KWIB21	1	BC63791_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	ATGAACAGTCTATCCTCCTTTAG	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63791_Msal_Bot2012_COI	KWIB21	1	BC63791_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	GYTAGTTCCRATGATGTTAGCTG	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63791_Pvir_Dia2013_COI	KWIB21	1	BC63791_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	CTTAGTGGCATTAATTCGDAATCC	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63791_Rhar_Sim2018_COI	KWIB21	1	BC63791_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	CTGCTGCTATTGCTCATG	115.75679
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63793_Aamu_Bax2006_COI	KWIB18	1	BC63793_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	GCACAACCGGGATCTTTACTTC	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63793_Pcan_Bot2011_IGS	KWIB18	1	BC63793_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB18	IGS	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	CGTAATCCTCAGTACTGGCTA	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63793_Pper_Dia2013_COI	KWIB18	1	BC63793_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	CTTAGTGGCATTAATTCGDAATCC	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.506	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63793_Sspa_Oph2007_COI	KWIB18	1	BC63793_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	GAAGGCCTTCCGATGATC	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63793_Upin_Bot2011_COI	KWIB18	1	BC63793_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	CTTTAATTACAGCGTTTTTATTGTTGT	115.75699
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63794_Cjap_Sim2018_COI	KWIB17	1	BC63794_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63794_Mgig_Bot2012_COI	KWIB17	1	BC63794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	TCTTATTCGTTGGAGACTTTATAACCCT	115.75376
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63795_Cmae_Bot2011_COI	KWIB16	1	BC63795_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	ATGAACAGTCTATCCTCCTTTAG	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63795_Msal_Bot2012_COI	KWIB16	1	BC63795_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	GYTAGTTCCRATGATGTTAGCTG	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63795_Pvir_Dia2013_COI	KWIB16	1	BC63795_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	CTTAGTGGCATTAATTCGDAATCC	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63795_Rhar_Sim2018_COI	KWIB16	1	BC63795_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	CTGCTGCTATTGCTCATG	115.75743
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63797_Aamu_Bax2006_COI	KWIB13	1	BC63797_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	GCACAACCGGGATCTTTACTTC	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63797_Pcan_Bot2011_IGS	KWIB13	1	BC63797_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB13	IGS	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	CGTAATCCTCAGTACTGGCTA	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63797_Pper_Dia2013_COI	KWIB13	1	BC63797_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	CTTAGTGGCATTAATTCGDAATCC	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63797_Sspa_Oph2007_COI	KWIB13	1	BC63797_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	GAAGGCCTTCCGATGATC	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63797_Upin_Bot2011_COI	KWIB13	1	BC63797_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	CTTTAATTACAGCGTTTTTATTGTTGT	115.75209
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63798_Cjap_Sim2018_COI	KWIB12	1	BC63798_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63798_Mgig_Bot2012_COI	KWIB12	1	BC63798_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	TCTTATTCGTTGGAGACTTTATAACCCT	115.75291
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63799_Cmae_Bot2011_COI	KWIB11	1	BC63799_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	ATGAACAGTCTATCCTCCTTTAG	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63799_Msal_Bot2012_COI	KWIB11	1	BC63799_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	GYTAGTTCCRATGATGTTAGCTG	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63799_Pvir_Dia2013_COI	KWIB11	1	BC63799_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	CTTAGTGGCATTAATTCGDAATCC	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63799_Rhar_Sim2018_COI	KWIB11	1	BC63799_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	CTGCTGCTATTGCTCATG	115.74522
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63801_Aamu_Bax2006_COI	KWIB8	1	BC63801_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	GCACAACCGGGATCTTTACTTC	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63801_Pcan_Bot2011_IGS	KWIB8	1	BC63801_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB8	IGS	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	CGTAATCCTCAGTACTGGCTA	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63801_Pper_Dia2013_COI	KWIB8	1	BC63801_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	CTTAGTGGCATTAATTCGDAATCC	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63801_Sspa_Oph2007_COI	KWIB8	1	BC63801_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	GAAGGCCTTCCGATGATC	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63801_Upin_Bot2011_COI	KWIB8	1	BC63801_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	CTTTAATTACAGCGTTTTTATTGTTGT	115.74448
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63802_Cjap_Sim2018_COI	KWIB7	1	BC63802_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63802_Mgig_Bot2012_COI	KWIB7	1	BC63802_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	TCTTATTCGTTGGAGACTTTATAACCCT	115.74276
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63803_Cmae_Bot2011_COI	KWIB6	1	BC63803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	ATGAACAGTCTATCCTCCTTTAG	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63803_Msal_Bot2012_COI	KWIB6	1	BC63803_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	GYTAGTTCCRATGATGTTAGCTG	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63803_Pvir_Dia2013_COI	KWIB6	1	BC63803_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	CTTAGTGGCATTAATTCGDAATCC	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63803_Rhar_Sim2018_COI	KWIB6	1	BC63803_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	CTGCTGCTATTGCTCATG	115.74078
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63805_Aamu_Bax2006_COI	KWIB3	1	BC63805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	GCACAACCGGGATCTTTACTTC	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63805_Pcan_Bot2011_IGS	KWIB3	1	BC63805_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB3	IGS	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	CGTAATCCTCAGTACTGGCTA	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63805_Pper_Dia2013_COI	KWIB3	1	BC63805_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	CTTAGTGGCATTAATTCGDAATCC	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63805_Sspa_Oph2007_COI	KWIB3	1	BC63805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	GAAGGCCTTCCGATGATC	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63805_Upin_Bot2011_COI	KWIB3	1	BC63805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	CTTTAATTACAGCGTTTTTATTGTTGT	115.74123
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63806_Cjap_Sim2018_COI	KWIB2	1	BC63806_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63806_Mgig_Bot2012_COI	KWIB2	1	BC63806_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	TCTTATTCGTTGGAGACTTTATAACCCT	115.74169
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63807_Cmae_Bot2011_COI	KWIB1	1	BC63807_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	ATGAACAGTCTATCCTCCTTTAG	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63807_Msal_Bot2012_COI	KWIB1	1	BC63807_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	GYTAGTTCCRATGATGTTAGCTG	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63807_Pvir_Dia2013_COI	KWIB1	1	BC63807_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	CTTAGTGGCATTAATTCGDAATCC	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63807_Rhar_Sim2018_COI	KWIB1	1	BC63807_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	CTGCTGCTATTGCTCATG	115.74057
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63809_Aamu_Bax2006_COI	BUNB15	1	BC63809_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	GCACAACCGGGATCTTTACTTC	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63809_Pcan_Bot2011_IGS	BUNB15	1	BC63809_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB15	IGS	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	CGTAATCCTCAGTACTGGCTA	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63809_Pper_Dia2013_COI	BUNB15	1	BC63809_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	CTTAGTGGCATTAATTCGDAATCC	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63809_Sspa_Oph2007_COI	BUNB15	1	BC63809_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	GAAGGCCTTCCGATGATC	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63809_Upin_Bot2011_COI	BUNB15	1	BC63809_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	CTTTAATTACAGCGTTTTTATTGTTGT	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.32211	WGS84	BC63809	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63809_Vgib_Wil2025_COI	BUNB15	1	BC63809_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	CCCCTCTTTACAAGTTCTTTAGGGT	115.66455
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63810_Cmae_Bot2011_COI	BUNB10	1	BC63810_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	ATGAACAGTCTATCCTCCTTTAG	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63810_Msal_Bot2012_COI	BUNB10	1	BC63810_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	GYTAGTTCCRATGATGTTAGCTG	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63810_Pvir_Dia2013_COI	BUNB10	1	BC63810_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	CTTAGTGGCATTAATTCGDAATCC	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63810_Rhar_Sim2018_COI	BUNB10	1	BC63810_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	CTGCTGCTATTGCTCATG	115.66527
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63812_Aamu_Bax2006_COI	BUNB23	1	BC63812_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	GCACAACCGGGATCTTTACTTC	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63812_Pcan_Bot2011_IGS	BUNB23	1	BC63812_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB23	IGS	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	CGTAATCCTCAGTACTGGCTA	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63812_Pper_Dia2013_COI	BUNB23	1	BC63812_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	CTTAGTGGCATTAATTCGDAATCC	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63812_Sspa_Oph2007_COI	BUNB23	1	BC63812_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	GAAGGCCTTCCGATGATC	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63812_Upin_Bot2011_COI	BUNB23	1	BC63812_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	CTTTAATTACAGCGTTTTTATTGTTGT	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.30654	WGS84	BC63812	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63812_Vgib_Wil2025_COI	BUNB23	1	BC63812_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	CCCCTCTTTACAAGTTCTTTAGGGT	115.64711
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63813_Cjap_Sim2018_COI	BUNB22	1	BC63813_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63813_Mgig_Bot2012_COI	BUNB22	1	BC63813_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	TCTTATTCGTTGGAGACTTTATAACCCT	115.64537
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63814_Cmae_Bot2011_COI	BUNB21	1	BC63814_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	ATGAACAGTCTATCCTCCTTTAG	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63814_Msal_Bot2012_COI	BUNB21	1	BC63814_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	GYTAGTTCCRATGATGTTAGCTG	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63814_Pvir_Dia2013_COI	BUNB21	1	BC63814_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	CTTAGTGGCATTAATTCGDAATCC	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63814_Rhar_Sim2018_COI	BUNB21	1	BC63814_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	CTGCTGCTATTGCTCATG	115.64716
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63816_Aamu_Bax2006_COI	BUNB18	1	BC63816_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	GCACAACCGGGATCTTTACTTC	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63816_Pcan_Bot2011_IGS	BUNB18	1	BC63816_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB18	IGS	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	CGTAATCCTCAGTACTGGCTA	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63816_Pper_Dia2013_COI	BUNB18	1	BC63816_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	CTTAGTGGCATTAATTCGDAATCC	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63816_Sspa_Oph2007_COI	BUNB18	1	BC63816_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	GAAGGCCTTCCGATGATC	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63816_Upin_Bot2011_COI	BUNB18	1	BC63816_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	CTTTAATTACAGCGTTTTTATTGTTGT	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63816_Vgib_Wil2025_COI	BUNB18	1	BC63816_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	CCCCTCTTTACAAGTTCTTTAGGGT	115.64277
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63817_Cjap_Sim2018_COI	BUNB17	1	BC63817_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63817_Mgig_Bot2012_COI	BUNB17	1	BC63817_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	TCTTATTCGTTGGAGACTTTATAACCCT	115.66296
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63818_Cmae_Bot2011_COI	BUNB16	1	BC63818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	ATGAACAGTCTATCCTCCTTTAG	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63818_Msal_Bot2012_COI	BUNB16	1	BC63818_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	GYTAGTTCCRATGATGTTAGCTG	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63818_Pvir_Dia2013_COI	BUNB16	1	BC63818_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	CTTAGTGGCATTAATTCGDAATCC	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63818_Rhar_Sim2018_COI	BUNB16	1	BC63818_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	CTGCTGCTATTGCTCATG	115.6614
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63820_Asen_Wil2025_COI	BUNB13	1	BC63820_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	GTTGTTGTTACAACACATGCTCTTTTG	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63820_Cjap_Sim2018_COI	BUNB13	1	BC63820_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63820_Mgig_Bot2012_COI	BUNB13	1	BC63820_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	TCTTATTCGTTGGAGACTTTATAACCCT	115.66204
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63821_Cmae_Bot2011_COI	BUNB12	1	BC63821_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	ATGAACAGTCTATCCTCCTTTAG	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63821_Msal_Bot2012_COI	BUNB12	1	BC63821_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	GYTAGTTCCRATGATGTTAGCTG	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63821_Pvir_Dia2013_COI	BUNB12	1	BC63821_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	CTTAGTGGCATTAATTCGDAATCC	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63821_Rhar_Sim2018_COI	BUNB12	1	BC63821_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	CTGCTGCTATTGCTCATG	115.66029
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63823_Aamu_Bax2006_COI	BUNB9	1	BC63823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	GCACAACCGGGATCTTTACTTC	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63823_Pcan_Bot2011_IGS	BUNB9	1	BC63823_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB9	IGS	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	CGTAATCCTCAGTACTGGCTA	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63823_Pper_Dia2013_COI	BUNB9	1	BC63823_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	CTTAGTGGCATTAATTCGDAATCC	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63823_Sspa_Oph2007_COI	BUNB9	1	BC63823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	GAAGGCCTTCCGATGATC	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63823_Upin_Bot2011_COI	BUNB9	1	BC63823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	CTTTAATTACAGCGTTTTTATTGTTGT	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63823_Vgib_Wil2025_COI	BUNB9	1	BC63823_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	CCCCTCTTTACAAGTTCTTTAGGGT	115.66373
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63824_Asen_Wil2025_COI	BUNB8	1	BC63824_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	GTTGTTGTTACAACACATGCTCTTTTG	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63824_Cjap_Sim2018_COI	BUNB8	1	BC63824_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63824_Mgig_Bot2012_COI	BUNB8	1	BC63824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	TCTTATTCGTTGGAGACTTTATAACCCT	115.66286
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63825_Cmae_Bot2011_COI	BUNB7	1	BC63825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	ATGAACAGTCTATCCTCCTTTAG	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63825_Msal_Bot2012_COI	BUNB7	1	BC63825_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	GYTAGTTCCRATGATGTTAGCTG	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63825_Pvir_Dia2013_COI	BUNB7	1	BC63825_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	CTTAGTGGCATTAATTCGDAATCC	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63825_Rhar_Sim2018_COI	BUNB7	1	BC63825_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	CTGCTGCTATTGCTCATG	115.66108
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63827_Aamu_Bax2006_COI	BUNB4	1	BC63827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	GCACAACCGGGATCTTTACTTC	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63827_Pcan_Bot2011_IGS	BUNB4	1	BC63827_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB4	IGS	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	CGTAATCCTCAGTACTGGCTA	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63827_Pper_Dia2013_COI	BUNB4	1	BC63827_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	CTTAGTGGCATTAATTCGDAATCC	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.816	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63827_Sspa_Oph2007_COI	BUNB4	1	BC63827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Present	Sample	Sspa_Oph2007_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	GAAGGCCTTCCGATGATC	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63827_Upin_Bot2011_COI	BUNB4	1	BC63827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	CTTTAATTACAGCGTTTTTATTGTTGT	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63827_Vgib_Wil2025_COI	BUNB4	1	BC63827_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	CCCCTCTTTACAAGTTCTTTAGGGT	115.66528
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63828_Asen_Wil2025_COI	BUNB3	1	BC63828_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	GTTGTTGTTACAACACATGCTCTTTTG	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63828_Cjap_Sim2018_COI	BUNB3	1	BC63828_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63828_Mgig_Bot2012_COI	BUNB3	1	BC63828_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	TCTTATTCGTTGGAGACTTTATAACCCT	115.66363
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63829_Cmae_Bot2011_COI	BUNB2	1	BC63829_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	ATGAACAGTCTATCCTCCTTTAG	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63829_Msal_Bot2012_COI	BUNB2	1	BC63829_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	GYTAGTTCCRATGATGTTAGCTG	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63829_Pvir_Dia2013_COI	BUNB2	1	BC63829_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	CTTAGTGGCATTAATTCGDAATCC	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63829_Rhar_Sim2018_COI	BUNB2	1	BC63829_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	CTGCTGCTATTGCTCATG	115.66723
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63830_Aamu_Bax2006_COI	BUNB1	1	BC63830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	GCACAACCGGGATCTTTACTTC	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63830_Pcan_Bot2011_IGS	BUNB1	1	BC63830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB1	IGS	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	CGTAATCCTCAGTACTGGCTA	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63830_Pper_Dia2013_COI	BUNB1	1	BC63830_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	CTTAGTGGCATTAATTCGDAATCC	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63830_Sspa_Oph2007_COI	BUNB1	1	BC63830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	GAAGGCCTTCCGATGATC	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63830_Upin_Bot2011_COI	BUNB1	1	BC63830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	CTTTAATTACAGCGTTTTTATTGTTGT	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63830_Vgib_Wil2025_COI	BUNB1	1	BC63830_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	CCCCTCTTTACAAGTTCTTTAGGGT	115.66652
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63831_Asen_Wil2025_COI	GERB15	1	BC63831_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Asen_Wil2025_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	GTTGTTGTTACAACACATGCTCTTTTG	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63831_Cjap_Sim2018_COI	GERB15	1	BC63831_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63831_Mgig_Bot2012_COI	GERB15	1	BC63831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	TCTTATTCGTTGGAGACTTTATAACCCT	114.60004
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63832_Cmae_Bot2011_COI	GERB10	1	BC63832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	ATGAACAGTCTATCCTCCTTTAG	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63832_Msal_Bot2012_COI	GERB10	1	BC63832_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Msal_Bot2012_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	GYTAGTTCCRATGATGTTAGCTG	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63832_Pvir_Dia2013_COI	GERB10	1	BC63832_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	CTTAGTGGCATTAATTCGDAATCC	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63832_Rhar_Sim2018_COI	GERB10	1	BC63832_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	CTGCTGCTATTGCTCATG	114.60076
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63834_Aamu_Bax2006_COI	GERB14	1	BC63834_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	GCACAACCGGGATCTTTACTTC	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63834_Pcan_Bot2011_IGS	GERB14	1	BC63834_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB14	IGS	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	CGTAATCCTCAGTACTGGCTA	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63834_Pper_Dia2013_COI	GERB14	1	BC63834_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Pper_Dia2013_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	CTTAGTGGCATTAATTCGDAATCC	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63834_Sspa_Oph2007_COI	GERB14	1	BC63834_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	GAAGGCCTTCCGATGATC	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63834_Upin_Bot2011_COI	GERB14	1	BC63834_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Upin_Bot2011_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	CTTTAATTACAGCGTTTTTATTGTTGT	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.7731	WGS84	BC63834	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63834_Vgib_Wil2025_COI	GERB14	1	BC63834_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	CCCCTCTTTACAAGTTCTTTAGGGT	114.60041
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77301	WGS84	BC63835	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63835_Asen_Wil2025_COI	GERB13	1	BC63835_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Asen_Wil2025_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	GTTGTTGTTACAACACATGCTCTTTTG	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63835_Cjap_Sim2018_COI	GERB13	1	BC63835_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59874
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63836_Cmae_Bot2011_COI	GERB12	1	BC63836_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	ATGAACAGTCTATCCTCCTTTAG	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63836_Msal_Bot2012_COI	GERB12	1	BC63836_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Msal_Bot2012_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	GYTAGTTCCRATGATGTTAGCTG	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63836_Pvir_Dia2013_COI	GERB12	1	BC63836_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	CTTAGTGGCATTAATTCGDAATCC	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63836_Rhar_Sim2018_COI	GERB12	1	BC63836_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	CTGCTGCTATTGCTCATG	114.59728
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63838_Aamu_Bax2006_COI	GERB9	1	BC63838_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	GCACAACCGGGATCTTTACTTC	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63838_Pcan_Bot2011_IGS	GERB9	1	BC63838_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB9	IGS	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	CGTAATCCTCAGTACTGGCTA	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63838_Pper_Dia2013_COI	GERB9	1	BC63838_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Pper_Dia2013_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	CTTAGTGGCATTAATTCGDAATCC	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63838_Sspa_Oph2007_COI	GERB9	1	BC63838_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	GAAGGCCTTCCGATGATC	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63838_Upin_Bot2011_COI	GERB9	1	BC63838_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Upin_Bot2011_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	CTTTAATTACAGCGTTTTTATTGTTGT	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.7742	WGS84	BC63838	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63838_Vgib_Wil2025_COI	GERB9	1	BC63838_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	CCCCTCTTTACAAGTTCTTTAGGGT	114.59939
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77384	WGS84	BC63839	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63839_Asen_Wil2025_COI	GERB8	1	BC63839_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Asen_Wil2025_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	GTTGTTGTTACAACACATGCTCTTTTG	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63839_Cjap_Sim2018_COI	GERB8	1	BC63839_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63839_Mgig_Bot2012_COI	GERB8	1	BC63839_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	TCTTATTCGTTGGAGACTTTATAACCCT	114.59782
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63841_Aamu_Bax2006_COI	GERB6	1	BC63841_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	GCACAACCGGGATCTTTACTTC	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63841_Pcan_Bot2011_IGS	GERB6	1	BC63841_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB6	IGS	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	CGTAATCCTCAGTACTGGCTA	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63841_Pper_Dia2013_COI	GERB6	1	BC63841_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Pper_Dia2013_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	CTTAGTGGCATTAATTCGDAATCC	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63841_Sspa_Oph2007_COI	GERB6	1	BC63841_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	GAAGGCCTTCCGATGATC	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63841_Upin_Bot2011_COI	GERB6	1	BC63841_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Upin_Bot2011_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	CTTTAATTACAGCGTTTTTATTGTTGT	114.59995
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63842_Cjap_Sim2018_COI	GERB4	1	BC63842_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63842_Mgig_Bot2012_COI	GERB4	1	BC63842_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	TCTTATTCGTTGGAGACTTTATAACCCT	114.59693
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63843_Cmae_Bot2011_COI	GERB3	1	BC63843_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	ATGAACAGTCTATCCTCCTTTAG	114.59538
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63843_Msal_Bot2012_COI	GERB3	1	BC63843_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Msal_Bot2012_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	GYTAGTTCCRATGATGTTAGCTG	114.59538
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63843_Pvir_Dia2013_COI	GERB3	1	BC63843_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	CTTAGTGGCATTAATTCGDAATCC	114.59538
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63843_Rhar_Sim2018_COI	GERB3	1	BC63843_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	CTGCTGCTATTGCTCATG	114.59538
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63845_Aamu_Bax2006_COI	KWIA60	1	BC63845_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	GCACAACCGGGATCTTTACTTC	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63845_Pcan_Bot2011_IGS	KWIA60	1	BC63845_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA60	IGS	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	CGTAATCCTCAGTACTGGCTA	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63845_Pper_Dia2013_COI	KWIA60	1	BC63845_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	CTTAGTGGCATTAATTCGDAATCC	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63845_Sspa_Oph2007_COI	KWIA60	1	BC63845_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	GAAGGCCTTCCGATGATC	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63845_Upin_Bot2011_COI	KWIA60	1	BC63845_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	CTTTAATTACAGCGTTTTTATTGTTGT	115.7731
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63846_Cjap_Sim2018_COI	KWIA59	1	BC63846_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63846_Mgig_Bot2012_COI	KWIA59	1	BC63846_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	TCTTATTCGTTGGAGACTTTATAACCCT	115.77156
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63847_Cmae_Bot2011_COI	KWIA58	1	BC63847_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	ATGAACAGTCTATCCTCCTTTAG	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63847_Msal_Bot2012_COI	KWIA58	1	BC63847_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	GYTAGTTCCRATGATGTTAGCTG	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63847_Pvir_Dia2013_COI	KWIA58	1	BC63847_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	CTTAGTGGCATTAATTCGDAATCC	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63847_Rhar_Sim2018_COI	KWIA58	1	BC63847_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	CTGCTGCTATTGCTCATG	115.76965
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63849_Aamu_Bax2006_COI	KWIA56	1	BC63849_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	GCACAACCGGGATCTTTACTTC	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63849_Pcan_Bot2011_IGS	KWIA56	1	BC63849_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA56	IGS	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	CGTAATCCTCAGTACTGGCTA	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63849_Pper_Dia2013_COI	KWIA56	1	BC63849_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	CTTAGTGGCATTAATTCGDAATCC	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63849_Sspa_Oph2007_COI	KWIA56	1	BC63849_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	GAAGGCCTTCCGATGATC	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63849_Upin_Bot2011_COI	KWIA56	1	BC63849_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	CTTTAATTACAGCGTTTTTATTGTTGT	115.77242
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63850_Cmae_Bot2011_COI	KWIA55	1	BC63850_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	ATGAACAGTCTATCCTCCTTTAG	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63850_Msal_Bot2012_COI	KWIA55	1	BC63850_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	GYTAGTTCCRATGATGTTAGCTG	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63850_Pvir_Dia2013_COI	KWIA55	1	BC63850_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	CTTAGTGGCATTAATTCGDAATCC	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63850_Rhar_Sim2018_COI	KWIA55	1	BC63850_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	CTGCTGCTATTGCTCATG	115.77384
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63852_Aamu_Bax2006_COI	KWIA53	1	BC63852_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	GCACAACCGGGATCTTTACTTC	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63852_Pcan_Bot2011_IGS	KWIA53	1	BC63852_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA53	IGS	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	CGTAATCCTCAGTACTGGCTA	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63852_Pper_Dia2013_COI	KWIA53	1	BC63852_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	CTTAGTGGCATTAATTCGDAATCC	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63852_Sspa_Oph2007_COI	KWIA53	1	BC63852_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	GAAGGCCTTCCGATGATC	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63852_Upin_Bot2011_COI	KWIA53	1	BC63852_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	CTTTAATTACAGCGTTTTTATTGTTGT	115.77131
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63853_Cjap_Sim2018_COI	KWIA52	1	BC63853_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63853_Mgig_Bot2012_COI	KWIA52	1	BC63853_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	TCTTATTCGTTGGAGACTTTATAACCCT	115.76936
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63854_Cmae_Bot2011_COI	KWIA51	1	BC63854_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	ATGAACAGTCTATCCTCCTTTAG	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63854_Msal_Bot2012_COI	KWIA51	1	BC63854_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	GYTAGTTCCRATGATGTTAGCTG	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63854_Pvir_Dia2013_COI	KWIA51	1	BC63854_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	CTTAGTGGCATTAATTCGDAATCC	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63854_Rhar_Sim2018_COI	KWIA51	1	BC63854_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	CTGCTGCTATTGCTCATG	115.76603
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63856_Aamu_Bax2006_COI	KWIA49	1	BC63856_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	GCACAACCGGGATCTTTACTTC	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63856_Pcan_Bot2011_IGS	KWIA49	1	BC63856_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA49	IGS	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	CGTAATCCTCAGTACTGGCTA	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63856_Pper_Dia2013_COI	KWIA49	1	BC63856_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	CTTAGTGGCATTAATTCGDAATCC	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63856_Sspa_Oph2007_COI	KWIA49	1	BC63856_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	GAAGGCCTTCCGATGATC	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63856_Upin_Bot2011_COI	KWIA49	1	BC63856_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	CTTTAATTACAGCGTTTTTATTGTTGT	115.76479
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63857_Cjap_Sim2018_COI	KWIA48	1	BC63857_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63857_Mgig_Bot2012_COI	KWIA48	1	BC63857_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	TCTTATTCGTTGGAGACTTTATAACCCT	115.76244
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63858_Cmae_Bot2011_COI	KWIA47	1	BC63858_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	ATGAACAGTCTATCCTCCTTTAG	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63858_Msal_Bot2012_COI	KWIA47	1	BC63858_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	GYTAGTTCCRATGATGTTAGCTG	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63858_Pvir_Dia2013_COI	KWIA47	1	BC63858_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	CTTAGTGGCATTAATTCGDAATCC	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63858_Rhar_Sim2018_COI	KWIA47	1	BC63858_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	CTGCTGCTATTGCTCATG	115.76541
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63860_Cjap_Sim2018_COI	KWIA45	1	BC63860_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63860_Mgig_Bot2012_COI	KWIA45	1	BC63860_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	TCTTATTCGTTGGAGACTTTATAACCCT	115.76077
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63861_Cmae_Bot2011_COI	KWIA44	1	BC63861_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	ATGAACAGTCTATCCTCCTTTAG	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63861_Msal_Bot2012_COI	KWIA44	1	BC63861_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	GYTAGTTCCRATGATGTTAGCTG	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63861_Pvir_Dia2013_COI	KWIA44	1	BC63861_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	CTTAGTGGCATTAATTCGDAATCC	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63861_Rhar_Sim2018_COI	KWIA44	1	BC63861_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	CTGCTGCTATTGCTCATG	115.76186
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63863_Aamu_Bax2006_COI	KWIA42	1	BC63863_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	GCACAACCGGGATCTTTACTTC	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63863_Pcan_Bot2011_IGS	KWIA42	1	BC63863_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA42	IGS	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	CGTAATCCTCAGTACTGGCTA	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63863_Pper_Dia2013_COI	KWIA42	1	BC63863_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	CTTAGTGGCATTAATTCGDAATCC	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63863_Sspa_Oph2007_COI	KWIA42	1	BC63863_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	GAAGGCCTTCCGATGATC	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63863_Upin_Bot2011_COI	KWIA42	1	BC63863_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	CTTTAATTACAGCGTTTTTATTGTTGT	115.75685
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63864_Cjap_Sim2018_COI	KWIA41	1	BC63864_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63864_Mgig_Bot2012_COI	KWIA41	1	BC63864_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	TCTTATTCGTTGGAGACTTTATAACCCT	115.75511
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63865_Cmae_Bot2011_COI	KWIA40	1	BC63865_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	ATGAACAGTCTATCCTCCTTTAG	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63865_Msal_Bot2012_COI	KWIA40	1	BC63865_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	GYTAGTTCCRATGATGTTAGCTG	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63865_Pvir_Dia2013_COI	KWIA40	1	BC63865_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	CTTAGTGGCATTAATTCGDAATCC	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63865_Rhar_Sim2018_COI	KWIA40	1	BC63865_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	CTGCTGCTATTGCTCATG	115.75719
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63867_Aamu_Bax2006_COI	KWIA38	1	BC63867_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	GCACAACCGGGATCTTTACTTC	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63867_Pcan_Bot2011_IGS	KWIA38	1	BC63867_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA38	IGS	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	CGTAATCCTCAGTACTGGCTA	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63867_Pper_Dia2013_COI	KWIA38	1	BC63867_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	CTTAGTGGCATTAATTCGDAATCC	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63867_Sspa_Oph2007_COI	KWIA38	1	BC63867_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	GAAGGCCTTCCGATGATC	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63867_Upin_Bot2011_COI	KWIA38	1	BC63867_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	CTTTAATTACAGCGTTTTTATTGTTGT	115.75354
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63868_Cjap_Sim2018_COI	KWIA37	1	BC63868_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63868_Mgig_Bot2012_COI	KWIA37	1	BC63868_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	TCTTATTCGTTGGAGACTTTATAACCCT	115.75622
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63869_Cmae_Bot2011_COI	KWIA36	1	BC63869_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	ATGAACAGTCTATCCTCCTTTAG	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63869_Msal_Bot2012_COI	KWIA36	1	BC63869_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	GYTAGTTCCRATGATGTTAGCTG	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63869_Pvir_Dia2013_COI	KWIA36	1	BC63869_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	CTTAGTGGCATTAATTCGDAATCC	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63869_Rhar_Sim2018_COI	KWIA36	1	BC63869_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	CTGCTGCTATTGCTCATG	115.75422
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63870_Aamu_Bax2006_COI	KWIA35	1	BC63870_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	GCACAACCGGGATCTTTACTTC	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63870_Pcan_Bot2011_IGS	KWIA35	1	BC63870_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA35	IGS	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	CGTAATCCTCAGTACTGGCTA	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63870_Pper_Dia2013_COI	KWIA35	1	BC63870_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	CTTAGTGGCATTAATTCGDAATCC	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63870_Sspa_Oph2007_COI	KWIA35	1	BC63870_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	GAAGGCCTTCCGATGATC	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63870_Upin_Bot2011_COI	KWIA35	1	BC63870_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	CTTTAATTACAGCGTTTTTATTGTTGT	115.757
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63871_Cjap_Sim2018_COI	KWIA34	1	BC63871_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63871_Mgig_Bot2012_COI	KWIA34	1	BC63871_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	TCTTATTCGTTGGAGACTTTATAACCCT	115.75462
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63872_Cmae_Bot2011_COI	KWIA33	1	BC63872_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	ATGAACAGTCTATCCTCCTTTAG	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63872_Msal_Bot2012_COI	KWIA33	1	BC63872_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	GYTAGTTCCRATGATGTTAGCTG	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63872_Pvir_Dia2013_COI	KWIA33	1	BC63872_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	CTTAGTGGCATTAATTCGDAATCC	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63872_Rhar_Sim2018_COI	KWIA33	1	BC63872_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	CTGCTGCTATTGCTCATG	115.75344
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63874_Aamu_Bax2006_COI	KWIA31	1	BC63874_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	GCACAACCGGGATCTTTACTTC	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63874_Pcan_Bot2011_IGS	KWIA31	1	BC63874_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA31	IGS	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	CGTAATCCTCAGTACTGGCTA	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63874_Pper_Dia2013_COI	KWIA31	1	BC63874_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	CTTAGTGGCATTAATTCGDAATCC	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63874_Sspa_Oph2007_COI	KWIA31	1	BC63874_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	GAAGGCCTTCCGATGATC	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63874_Upin_Bot2011_COI	KWIA31	1	BC63874_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	CTTTAATTACAGCGTTTTTATTGTTGT	115.7577
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63875_Cjap_Sim2018_COI	KWIA30	1	BC63875_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63875_Mgig_Bot2012_COI	KWIA30	1	BC63875_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	TCTTATTCGTTGGAGACTTTATAACCCT	115.75568
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63876_Cmae_Bot2011_COI	KWIA29	1	BC63876_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	ATGAACAGTCTATCCTCCTTTAG	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63876_Msal_Bot2012_COI	KWIA29	1	BC63876_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	GYTAGTTCCRATGATGTTAGCTG	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63876_Pvir_Dia2013_COI	KWIA29	1	BC63876_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	CTTAGTGGCATTAATTCGDAATCC	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63876_Rhar_Sim2018_COI	KWIA29	1	BC63876_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	CTGCTGCTATTGCTCATG	115.75383
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63878_Aamu_Bax2006_COI	KWIA27	1	BC63878_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	GCACAACCGGGATCTTTACTTC	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63878_Pcan_Bot2011_IGS	KWIA27	1	BC63878_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA27	IGS	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	CGTAATCCTCAGTACTGGCTA	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63878_Pper_Dia2013_COI	KWIA27	1	BC63878_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	CTTAGTGGCATTAATTCGDAATCC	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63878_Sspa_Oph2007_COI	KWIA27	1	BC63878_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	GAAGGCCTTCCGATGATC	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63878_Upin_Bot2011_COI	KWIA27	1	BC63878_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	CTTTAATTACAGCGTTTTTATTGTTGT	115.7564
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63879_Cjap_Sim2018_COI	KWIA26	1	BC63879_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63879_Mgig_Bot2012_COI	KWIA26	1	BC63879_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	TCTTATTCGTTGGAGACTTTATAACCCT	115.75454
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63881_Aamu_Bax2006_COI	KWIA24	1	BC63881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	GCACAACCGGGATCTTTACTTC	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63881_Pcan_Bot2011_IGS	KWIA24	1	BC63881_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA24	IGS	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	CGTAATCCTCAGTACTGGCTA	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63881_Pper_Dia2013_COI	KWIA24	1	BC63881_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	CTTAGTGGCATTAATTCGDAATCC	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63881_Sspa_Oph2007_COI	KWIA24	1	BC63881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	GAAGGCCTTCCGATGATC	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63881_Upin_Bot2011_COI	KWIA24	1	BC63881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	CTTTAATTACAGCGTTTTTATTGTTGT	115.75717
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63882_Cjap_Sim2018_COI	KWIA23	1	BC63882_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63882_Mgig_Bot2012_COI	KWIA23	1	BC63882_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	TCTTATTCGTTGGAGACTTTATAACCCT	115.75331
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63883_Cmae_Bot2011_COI	KWIA22	1	BC63883_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	ATGAACAGTCTATCCTCCTTTAG	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63883_Msal_Bot2012_COI	KWIA22	1	BC63883_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	GYTAGTTCCRATGATGTTAGCTG	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63883_Pvir_Dia2013_COI	KWIA22	1	BC63883_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	CTTAGTGGCATTAATTCGDAATCC	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63883_Rhar_Sim2018_COI	KWIA22	1	BC63883_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	CTGCTGCTATTGCTCATG	115.7579
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63885_Aamu_Bax2006_COI	KWIA20	1	BC63885_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	GCACAACCGGGATCTTTACTTC	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63885_Pcan_Bot2011_IGS	KWIA20	1	BC63885_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA20	IGS	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	CGTAATCCTCAGTACTGGCTA	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63885_Pper_Dia2013_COI	KWIA20	1	BC63885_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	CTTAGTGGCATTAATTCGDAATCC	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63885_Sspa_Oph2007_COI	KWIA20	1	BC63885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	GAAGGCCTTCCGATGATC	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63885_Upin_Bot2011_COI	KWIA20	1	BC63885_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	CTTTAATTACAGCGTTTTTATTGTTGT	115.75401
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63886_Cjap_Sim2018_COI	KWIA19	1	BC63886_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63886_Mgig_Bot2012_COI	KWIA19	1	BC63886_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	TCTTATTCGTTGGAGACTTTATAACCCT	115.75228
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63887_Cmae_Bot2011_COI	KWIA18	1	BC63887_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	ATGAACAGTCTATCCTCCTTTAG	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63887_Msal_Bot2012_COI	KWIA18	1	BC63887_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	GYTAGTTCCRATGATGTTAGCTG	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63887_Pvir_Dia2013_COI	KWIA18	1	BC63887_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	CTTAGTGGCATTAATTCGDAATCC	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63887_Rhar_Sim2018_COI	KWIA18	1	BC63887_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	CTGCTGCTATTGCTCATG	115.757
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63889_Aamu_Bax2006_COI	KWIA16	1	BC63889_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	GCACAACCGGGATCTTTACTTC	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63889_Pcan_Bot2011_IGS	KWIA16	1	BC63889_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA16	IGS	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	CGTAATCCTCAGTACTGGCTA	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63889_Pper_Dia2013_COI	KWIA16	1	BC63889_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	CTTAGTGGCATTAATTCGDAATCC	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63889_Sspa_Oph2007_COI	KWIA16	1	BC63889_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	GAAGGCCTTCCGATGATC	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63889_Upin_Bot2011_COI	KWIA16	1	BC63889_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	CTTTAATTACAGCGTTTTTATTGTTGT	115.75755
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63890_Cmae_Bot2011_COI	KWIA15	1	BC63890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	ATGAACAGTCTATCCTCCTTTAG	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63890_Msal_Bot2012_COI	KWIA15	1	BC63890_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	GYTAGTTCCRATGATGTTAGCTG	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63890_Pvir_Dia2013_COI	KWIA15	1	BC63890_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	CTTAGTGGCATTAATTCGDAATCC	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63890_Rhar_Sim2018_COI	KWIA15	1	BC63890_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	CTGCTGCTATTGCTCATG	115.75557
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63892_Aamu_Bax2006_COI	KWIA13	1	BC63892_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	GCACAACCGGGATCTTTACTTC	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63892_Pcan_Bot2011_IGS	KWIA13	1	BC63892_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA13	IGS	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	CGTAATCCTCAGTACTGGCTA	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63892_Pper_Dia2013_COI	KWIA13	1	BC63892_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	CTTAGTGGCATTAATTCGDAATCC	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63892_Sspa_Oph2007_COI	KWIA13	1	BC63892_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	GAAGGCCTTCCGATGATC	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63892_Upin_Bot2011_COI	KWIA13	1	BC63892_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	CTTTAATTACAGCGTTTTTATTGTTGT	115.75187
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63893_Cjap_Sim2018_COI	KWIA12	1	BC63893_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63893_Mgig_Bot2012_COI	KWIA12	1	BC63893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	TCTTATTCGTTGGAGACTTTATAACCCT	115.75246
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63894_Cmae_Bot2011_COI	KWIA11	1	BC63894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	ATGAACAGTCTATCCTCCTTTAG	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63894_Msal_Bot2012_COI	KWIA11	1	BC63894_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	GYTAGTTCCRATGATGTTAGCTG	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63894_Pvir_Dia2013_COI	KWIA11	1	BC63894_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	CTTAGTGGCATTAATTCGDAATCC	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63894_Rhar_Sim2018_COI	KWIA11	1	BC63894_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	CTGCTGCTATTGCTCATG	115.74442
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63896_Aamu_Bax2006_COI	KWIA9	1	BC63896_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	GCACAACCGGGATCTTTACTTC	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63896_Pcan_Bot2011_IGS	KWIA9	1	BC63896_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA9	IGS	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	CGTAATCCTCAGTACTGGCTA	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63896_Pper_Dia2013_COI	KWIA9	1	BC63896_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	CTTAGTGGCATTAATTCGDAATCC	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63896_Sspa_Oph2007_COI	KWIA9	1	BC63896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	GAAGGCCTTCCGATGATC	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63896_Upin_Bot2011_COI	KWIA9	1	BC63896_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	CTTTAATTACAGCGTTTTTATTGTTGT	115.74164
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63897_Cjap_Sim2018_COI	KWIA8	1	BC63897_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63897_Mgig_Bot2012_COI	KWIA8	1	BC63897_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	TCTTATTCGTTGGAGACTTTATAACCCT	115.74373
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63898_Cmae_Bot2011_COI	KWIA7	1	BC63898_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	ATGAACAGTCTATCCTCCTTTAG	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63898_Msal_Bot2012_COI	KWIA7	1	BC63898_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	GYTAGTTCCRATGATGTTAGCTG	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63898_Pvir_Dia2013_COI	KWIA7	1	BC63898_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	CTTAGTGGCATTAATTCGDAATCC	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63898_Rhar_Sim2018_COI	KWIA7	1	BC63898_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	CTGCTGCTATTGCTCATG	115.74208
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63900_Aamu_Bax2006_COI	KWIA5	1	BC63900_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	GCACAACCGGGATCTTTACTTC	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63900_Pcan_Bot2011_IGS	KWIA5	1	BC63900_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA5	IGS	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	CGTAATCCTCAGTACTGGCTA	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63900_Pper_Dia2013_COI	KWIA5	1	BC63900_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	CTTAGTGGCATTAATTCGDAATCC	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63900_Sspa_Oph2007_COI	KWIA5	1	BC63900_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	GAAGGCCTTCCGATGATC	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63900_Upin_Bot2011_COI	KWIA5	1	BC63900_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	CTTTAATTACAGCGTTTTTATTGTTGT	115.74475
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63901_Cjap_Sim2018_COI	KWIA4	1	BC63901_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63901_Mgig_Bot2012_COI	KWIA4	1	BC63901_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	TCTTATTCGTTGGAGACTTTATAACCCT	115.74282
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63902_Cmae_Bot2011_COI	KWIA3	1	BC63902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	ATGAACAGTCTATCCTCCTTTAG	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63902_Msal_Bot2012_COI	KWIA3	1	BC63902_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	GYTAGTTCCRATGATGTTAGCTG	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63902_Pvir_Dia2013_COI	KWIA3	1	BC63902_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	CTTAGTGGCATTAATTCGDAATCC	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63902_Rhar_Sim2018_COI	KWIA3	1	BC63902_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	CTGCTGCTATTGCTCATG	115.74126
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63904_Aamu_Bax2006_COI	KWIA1	1	BC63904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	GCACAACCGGGATCTTTACTTC	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63904_Pcan_Bot2011_IGS	KWIA1	1	BC63904_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA1	IGS	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	CGTAATCCTCAGTACTGGCTA	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63904_Pper_Dia2013_COI	KWIA1	1	BC63904_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	CTTAGTGGCATTAATTCGDAATCC	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63904_Sspa_Oph2007_COI	KWIA1	1	BC63904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	GAAGGCCTTCCGATGATC	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63904_Upin_Bot2011_COI	KWIA1	1	BC63904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	CTTTAATTACAGCGTTTTTATTGTTGT	115.74031
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63905_Cjap_Sim2018_COI	FREA32	1	BC63905_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63905_Mgig_Bot2012_COI	FREA32	1	BC63905_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	TCTTATTCGTTGGAGACTTTATAACCCT	115.75126
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63906_Cmae_Bot2011_COI	FREA33	1	BC63906_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	ATGAACAGTCTATCCTCCTTTAG	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63906_Msal_Bot2012_COI	FREA33	1	BC63906_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Msal_Bot2012_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	GYTAGTTCCRATGATGTTAGCTG	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63906_Pvir_Dia2013_COI	FREA33	1	BC63906_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	CTTAGTGGCATTAATTCGDAATCC	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63906_Rhar_Sim2018_COI	FREA33	1	BC63906_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	CTGCTGCTATTGCTCATG	115.75228
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63908_Aamu_Bax2006_COI	FREA10	1	BC63908_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	GCACAACCGGGATCTTTACTTC	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63908_Pcan_Bot2011_IGS	FREA10	1	BC63908_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA10	IGS	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	CGTAATCCTCAGTACTGGCTA	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63908_Pper_Dia2013_COI	FREA10	1	BC63908_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Pper_Dia2013_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	CTTAGTGGCATTAATTCGDAATCC	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63908_Sspa_Oph2007_COI	FREA10	1	BC63908_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	GAAGGCCTTCCGATGATC	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63908_Upin_Bot2011_COI	FREA10	1	BC63908_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Upin_Bot2011_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	CTTTAATTACAGCGTTTTTATTGTTGT	115.74273
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63909_Cjap_Sim2018_COI	FREA15	1	BC63909_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63909_Esin_And2018_Cyb	FREA15	1	BC63909_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Esin_And2018_Cyb	FREA15	Cytb	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	ACCCCTCCTCATATCCAACCA	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63909_Mgig_Bot2012_COI	FREA15	1	BC63909_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	TCTTATTCGTTGGAGACTTTATAACCCT	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63909_Mjap_Gib2022_COI	FREA15	1	BC63909_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	AATTTAATTGTGACTAGGCATGGTCTG	115.74348
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63911_Aamu_Bax2006_COI	FREA25	1	BC63911_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	GCACAACCGGGATCTTTACTTC	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63911_Pcan_Bot2011_IGS	FREA25	1	BC63911_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA25	IGS	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	CGTAATCCTCAGTACTGGCTA	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63911_Pper_Dia2013_COI	FREA25	1	BC63911_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Pper_Dia2013_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	CTTAGTGGCATTAATTCGDAATCC	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63911_Sspa_Oph2007_COI	FREA25	1	BC63911_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	GAAGGCCTTCCGATGATC	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63911_Upin_Bot2011_COI	FREA25	1	BC63911_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Upin_Bot2011_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	CTTTAATTACAGCGTTTTTATTGTTGT	115.74809
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63912_Cjap_Sim2018_COI	FREA30	1	BC63912_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63912_Mgig_Bot2012_COI	FREA30	1	BC63912_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	TCTTATTCGTTGGAGACTTTATAACCCT	115.75018
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63913_Cmae_Bot2011_COI	FREA1	1	BC63913_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	ATGAACAGTCTATCCTCCTTTAG	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63913_Hsan_Wil2021_COI	FREA1	1	BC63913_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	CCTGGGCCGGTATAGTAGGT	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63913_Mros_Gib2022_COI	FREA1	1	BC63913_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Mros_Gib2022_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63913_Msal_Bot2012_COI	FREA1	1	BC63913_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Msal_Bot2012_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	GYTAGTTCCRATGATGTTAGCTG	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63913_Pvir_Dia2013_COI	FREA1	1	BC63913_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	CTTAGTGGCATTAATTCGDAATCC	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63913_Rhar_Sim2018_COI	FREA1	1	BC63913_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	CTGCTGCTATTGCTCATG	115.73752
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63915_Aamu_Bax2006_COI	FREA3	1	BC63915_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	GCACAACCGGGATCTTTACTTC	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63915_Pcan_Bot2011_IGS	FREA3	1	BC63915_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA3	IGS	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	CGTAATCCTCAGTACTGGCTA	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63915_Pper_Dia2013_COI	FREA3	1	BC63915_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Pper_Dia2013_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	CTTAGTGGCATTAATTCGDAATCC	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63915_Sspa_Oph2007_COI	FREA3	1	BC63915_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	GAAGGCCTTCCGATGATC	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63915_Upin_Bot2011_COI	FREA3	1	BC63915_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Upin_Bot2011_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	CTTTAATTACAGCGTTTTTATTGTTGT	115.73986
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63916_Cjap_Sim2018_COI	FREA4	1	BC63916_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63916_Esin_And2018_Cyb	FREA4	1	BC63916_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Esin_And2018_Cyb	FREA4	Cytb	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	ACCCCTCCTCATATCCAACCA	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63916_Mgig_Bot2012_COI	FREA4	1	BC63916_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	TCTTATTCGTTGGAGACTTTATAACCCT	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63916_Mjap_Gib2022_COI	FREA4	1	BC63916_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	AATTTAATTGTGACTAGGCATGGTCTG	115.74132
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63917_Cmae_Bot2011_COI	FREA6	1	BC63917_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	ATGAACAGTCTATCCTCCTTTAG	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63917_Hsan_Wil2021_COI	FREA6	1	BC63917_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	CCTGGGCCGGTATAGTAGGT	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63917_Mros_Gib2022_COI	FREA6	1	BC63917_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Mros_Gib2022_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63917_Msal_Bot2012_COI	FREA6	1	BC63917_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Msal_Bot2012_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	GYTAGTTCCRATGATGTTAGCTG	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63917_Pvir_Dia2013_COI	FREA6	1	BC63917_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	CTTAGTGGCATTAATTCGDAATCC	115.74243
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63919_Aamu_Bax2006_COI	FREA8	1	BC63919_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	GCACAACCGGGATCTTTACTTC	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63919_Pcan_Bot2011_IGS	FREA8	1	BC63919_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA8	IGS	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	CGTAATCCTCAGTACTGGCTA	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63919_Pper_Dia2013_COI	FREA8	1	BC63919_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Pper_Dia2013_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	CTTAGTGGCATTAATTCGDAATCC	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63919_Sspa_Oph2007_COI	FREA8	1	BC63919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	GAAGGCCTTCCGATGATC	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63919_Upin_Bot2011_COI	FREA8	1	BC63919_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Upin_Bot2011_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	CTTTAATTACAGCGTTTTTATTGTTGT	115.74336
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63920_Cmae_Bot2011_COI	FREA9	1	BC63920_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	ATGAACAGTCTATCCTCCTTTAG	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63920_Hsan_Wil2021_COI	FREA9	1	BC63920_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	CCTGGGCCGGTATAGTAGGT	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63920_Mros_Gib2022_COI	FREA9	1	BC63920_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Mros_Gib2022_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63920_Msal_Bot2012_COI	FREA9	1	BC63920_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Msal_Bot2012_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	GYTAGTTCCRATGATGTTAGCTG	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63920_Pvir_Dia2013_COI	FREA9	1	BC63920_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	CTTAGTGGCATTAATTCGDAATCC	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63920_Rhar_Sim2018_COI	FREA9	1	BC63920_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	CTGCTGCTATTGCTCATG	115.74142
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63922_Aamu_Bax2006_COI	FREA12	1	BC63922_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	GCACAACCGGGATCTTTACTTC	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63922_Pcan_Bot2011_IGS	FREA12	1	BC63922_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA12	IGS	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	CGTAATCCTCAGTACTGGCTA	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63922_Pper_Dia2013_COI	FREA12	1	BC63922_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Pper_Dia2013_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	CTTAGTGGCATTAATTCGDAATCC	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63922_Sspa_Oph2007_COI	FREA12	1	BC63922_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	GAAGGCCTTCCGATGATC	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63922_Upin_Bot2011_COI	FREA12	1	BC63922_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Upin_Bot2011_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	CTTTAATTACAGCGTTTTTATTGTTGT	115.74231
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63923_Cjap_Sim2018_COI	FREA13	1	BC63923_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63923_Esin_And2018_Cyb	FREA13	1	BC63923_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Esin_And2018_Cyb	FREA13	Cytb	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	ACCCCTCCTCATATCCAACCA	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63923_Mgig_Bot2012_COI	FREA13	1	BC63923_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	TCTTATTCGTTGGAGACTTTATAACCCT	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63923_Mjap_Gib2022_COI	FREA13	1	BC63923_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	AATTTAATTGTGACTAGGCATGGTCTG	115.7438
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63924_Cmae_Bot2011_COI	FREA14	1	BC63924_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	ATGAACAGTCTATCCTCCTTTAG	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63924_Hsan_Wil2021_COI	FREA14	1	BC63924_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	CCTGGGCCGGTATAGTAGGT	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63924_Mros_Gib2022_COI	FREA14	1	BC63924_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Mros_Gib2022_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63924_Msal_Bot2012_COI	FREA14	1	BC63924_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Msal_Bot2012_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	GYTAGTTCCRATGATGTTAGCTG	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63924_Pvir_Dia2013_COI	FREA14	1	BC63924_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	CTTAGTGGCATTAATTCGDAATCC	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63924_Rhar_Sim2018_COI	FREA14	1	BC63924_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	CTGCTGCTATTGCTCATG	115.74543
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63926_Aamu_Bax2006_COI	FREA21	1	BC63926_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	GCACAACCGGGATCTTTACTTC	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63926_Pcan_Bot2011_IGS	FREA21	1	BC63926_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA21	IGS	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	CGTAATCCTCAGTACTGGCTA	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63926_Pper_Dia2013_COI	FREA21	1	BC63926_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Pper_Dia2013_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	CTTAGTGGCATTAATTCGDAATCC	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63926_Sspa_Oph2007_COI	FREA21	1	BC63926_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	GAAGGCCTTCCGATGATC	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63926_Upin_Bot2011_COI	FREA21	1	BC63926_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Upin_Bot2011_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	CTTTAATTACAGCGTTTTTATTGTTGT	115.74536
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63927_Cjap_Sim2018_COI	FREA18	1	BC63927_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63927_Esin_And2018_Cyb	FREA18	1	BC63927_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Esin_And2018_Cyb	FREA18	Cytb	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	ACCCCTCCTCATATCCAACCA	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63927_Mgig_Bot2012_COI	FREA18	1	BC63927_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	TCTTATTCGTTGGAGACTTTATAACCCT	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63927_Mjap_Gib2022_COI	FREA18	1	BC63927_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	AATTTAATTGTGACTAGGCATGGTCTG	115.74433
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63928_Cmae_Bot2011_COI	FREA19	1	BC63928_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	ATGAACAGTCTATCCTCCTTTAG	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63928_Hsan_Wil2021_COI	FREA19	1	BC63928_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	CCTGGGCCGGTATAGTAGGT	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63928_Mros_Gib2022_COI	FREA19	1	BC63928_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Mros_Gib2022_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63928_Msal_Bot2012_COI	FREA19	1	BC63928_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Msal_Bot2012_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	GYTAGTTCCRATGATGTTAGCTG	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63928_Pvir_Dia2013_COI	FREA19	1	BC63928_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	CTTAGTGGCATTAATTCGDAATCC	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63928_Rhar_Sim2018_COI	FREA19	1	BC63928_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	CTGCTGCTATTGCTCATG	115.74597
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63930_Cjap_Sim2018_COI	FREA22	1	BC63930_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63930_Esin_And2018_Cyb	FREA22	1	BC63930_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Esin_And2018_Cyb	FREA22	Cytb	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	ACCCCTCCTCATATCCAACCA	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63930_Mgig_Bot2012_COI	FREA22	1	BC63930_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	TCTTATTCGTTGGAGACTTTATAACCCT	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63930_Mjap_Gib2022_COI	FREA22	1	BC63930_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	AATTTAATTGTGACTAGGCATGGTCTG	115.74689
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63931_Cmae_Bot2011_COI	FREA23	1	BC63931_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	ATGAACAGTCTATCCTCCTTTAG	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63931_Hsan_Wil2021_COI	FREA23	1	BC63931_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	CCTGGGCCGGTATAGTAGGT	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63931_Mros_Gib2022_COI	FREA23	1	BC63931_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Mros_Gib2022_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63931_Msal_Bot2012_COI	FREA23	1	BC63931_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Msal_Bot2012_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	GYTAGTTCCRATGATGTTAGCTG	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63931_Pvir_Dia2013_COI	FREA23	1	BC63931_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	CTTAGTGGCATTAATTCGDAATCC	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63931_Rhar_Sim2018_COI	FREA23	1	BC63931_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	CTGCTGCTATTGCTCATG	115.7487
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63933_Aamu_Bax2006_COI	FREA26	1	BC63933_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	GCACAACCGGGATCTTTACTTC	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63933_Pcan_Bot2011_IGS	FREA26	1	BC63933_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA26	IGS	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	CGTAATCCTCAGTACTGGCTA	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63933_Pper_Dia2013_COI	FREA26	1	BC63933_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Pper_Dia2013_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	CTTAGTGGCATTAATTCGDAATCC	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63933_Sspa_Oph2007_COI	FREA26	1	BC63933_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	GAAGGCCTTCCGATGATC	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63933_Upin_Bot2011_COI	FREA26	1	BC63933_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Upin_Bot2011_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	CTTTAATTACAGCGTTTTTATTGTTGT	115.74984
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63934_Cjap_Sim2018_COI	FREA27	1	BC63934_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63934_Mgig_Bot2012_COI	FREA27	1	BC63934_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	TCTTATTCGTTGGAGACTTTATAACCCT	115.74776
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63935_Cmae_Bot2011_COI	FREA28	1	BC63935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	ATGAACAGTCTATCCTCCTTTAG	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63935_Msal_Bot2012_COI	FREA28	1	BC63935_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Msal_Bot2012_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	GYTAGTTCCRATGATGTTAGCTG	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63935_Pvir_Dia2013_COI	FREA28	1	BC63935_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	CTTAGTGGCATTAATTCGDAATCC	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63935_Rhar_Sim2018_COI	FREA28	1	BC63935_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	CTGCTGCTATTGCTCATG	115.7493
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63937_Aamu_Bax2006_COI	FREA31	1	BC63937_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	GCACAACCGGGATCTTTACTTC	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63937_Pcan_Bot2011_IGS	FREA31	1	BC63937_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA31	IGS	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	CGTAATCCTCAGTACTGGCTA	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63937_Pper_Dia2013_COI	FREA31	1	BC63937_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Pper_Dia2013_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	CTTAGTGGCATTAATTCGDAATCC	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63937_Sspa_Oph2007_COI	FREA31	1	BC63937_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	GAAGGCCTTCCGATGATC	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63937_Upin_Bot2011_COI	FREA31	1	BC63937_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Upin_Bot2011_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	CTTTAATTACAGCGTTTTTATTGTTGT	115.75211
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63938_Cjap_Sim2018_COI	BUNA23	1	BC63938_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63938_Esin_And2018_Cyb	BUNA23	1	BC63938_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA23	Cytb	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	ACCCCTCCTCATATCCAACCA	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63938_Mgig_Bot2012_COI	BUNA23	1	BC63938_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	TCTTATTCGTTGGAGACTTTATAACCCT	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63938_Mjap_Gib2022_COI	BUNA23	1	BC63938_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	AATTTAATTGTGACTAGGCATGGTCTG	115.64785
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63939_Cmae_Bot2011_COI	BUNA22	1	BC63939_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	ATGAACAGTCTATCCTCCTTTAG	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63939_Hsan_Wil2021_COI	BUNA22	1	BC63939_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	CCTGGGCCGGTATAGTAGGT	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63939_Mros_Gib2022_COI	BUNA22	1	BC63939_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63939_Msal_Bot2012_COI	BUNA22	1	BC63939_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	GYTAGTTCCRATGATGTTAGCTG	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63939_Pvir_Dia2013_COI	BUNA22	1	BC63939_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	CTTAGTGGCATTAATTCGDAATCC	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63939_Rhar_Sim2018_COI	BUNA22	1	BC63939_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	CTGCTGCTATTGCTCATG	115.64643
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63940_Aamu_Bax2006_COI	BUNA21	1	BC63940_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	GCACAACCGGGATCTTTACTTC	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63940_Pcan_Bot2011_IGS	BUNA21	1	BC63940_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA21	IGS	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	CGTAATCCTCAGTACTGGCTA	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63940_Pper_Dia2013_COI	BUNA21	1	BC63940_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	CTTAGTGGCATTAATTCGDAATCC	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63940_Sspa_Oph2007_COI	BUNA21	1	BC63940_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	GAAGGCCTTCCGATGATC	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63940_Upin_Bot2011_COI	BUNA21	1	BC63940_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	CTTTAATTACAGCGTTTTTATTGTTGT	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63940_Vgib_Wil2025_COI	BUNA21	1	BC63940_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	CCCCTCTTTACAAGTTCTTTAGGGT	115.64669
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63941_Asen_Wil2025_COI	BUNA20	1	BC63941_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	GTTGTTGTTACAACACATGCTCTTTTG	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63941_Cjap_Sim2018_COI	BUNA20	1	BC63941_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63941_Esin_And2018_Cyb	BUNA20	1	BC63941_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA20	Cytb	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	ACCCCTCCTCATATCCAACCA	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63941_Mgig_Bot2012_COI	BUNA20	1	BC63941_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	TCTTATTCGTTGGAGACTTTATAACCCT	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63941_Mjap_Gib2022_COI	BUNA20	1	BC63941_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	AATTTAATTGTGACTAGGCATGGTCTG	115.64524
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63942_Cmae_Bot2011_COI	BUNA19	1	BC63942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	ATGAACAGTCTATCCTCCTTTAG	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63942_Hsan_Wil2021_COI	BUNA19	1	BC63942_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	CCTGGGCCGGTATAGTAGGT	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63942_Mros_Gib2022_COI	BUNA19	1	BC63942_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63942_Msal_Bot2012_COI	BUNA19	1	BC63942_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	GYTAGTTCCRATGATGTTAGCTG	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63942_Pvir_Dia2013_COI	BUNA19	1	BC63942_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	CTTAGTGGCATTAATTCGDAATCC	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63942_Rhar_Sim2018_COI	BUNA19	1	BC63942_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	CTGCTGCTATTGCTCATG	115.64384
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63944_Aamu_Bax2006_COI	BUNA17	1	BC63944_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	GCACAACCGGGATCTTTACTTC	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63944_Pcan_Bot2011_IGS	BUNA17	1	BC63944_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA17	IGS	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	CGTAATCCTCAGTACTGGCTA	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63944_Pper_Dia2013_COI	BUNA17	1	BC63944_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	CTTAGTGGCATTAATTCGDAATCC	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63944_Sspa_Oph2007_COI	BUNA17	1	BC63944_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	GAAGGCCTTCCGATGATC	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63944_Upin_Bot2011_COI	BUNA17	1	BC63944_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	CTTTAATTACAGCGTTTTTATTGTTGT	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63944_Vgib_Wil2025_COI	BUNA17	1	BC63944_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	CCCCTCTTTACAAGTTCTTTAGGGT	115.66307
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63945_Asen_Wil2025_COI	BUNA16	1	BC63945_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	GTTGTTGTTACAACACATGCTCTTTTG	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63945_Cjap_Sim2018_COI	BUNA16	1	BC63945_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63945_Esin_And2018_Cyb	BUNA16	1	BC63945_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA16	Cytb	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	ACCCCTCCTCATATCCAACCA	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63945_Mgig_Bot2012_COI	BUNA16	1	BC63945_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	TCTTATTCGTTGGAGACTTTATAACCCT	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63945_Mjap_Gib2022_COI	BUNA16	1	BC63945_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	AATTTAATTGTGACTAGGCATGGTCTG	115.66118
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63946_Cmae_Bot2011_COI	BUNA15	1	BC63946_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	ATGAACAGTCTATCCTCCTTTAG	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63946_Hsan_Wil2021_COI	BUNA15	1	BC63946_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	CCTGGGCCGGTATAGTAGGT	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63946_Mros_Gib2022_COI	BUNA15	1	BC63946_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63946_Msal_Bot2012_COI	BUNA15	1	BC63946_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	GYTAGTTCCRATGATGTTAGCTG	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63946_Pvir_Dia2013_COI	BUNA15	1	BC63946_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	CTTAGTGGCATTAATTCGDAATCC	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63946_Rhar_Sim2018_COI	BUNA15	1	BC63946_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	CTGCTGCTATTGCTCATG	115.66542
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63948_Aamu_Bax2006_COI	BUNA13	1	BC63948_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	GCACAACCGGGATCTTTACTTC	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63948_Pcan_Bot2011_IGS	BUNA13	1	BC63948_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA13	IGS	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	CGTAATCCTCAGTACTGGCTA	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63948_Pper_Dia2013_COI	BUNA13	1	BC63948_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	CTTAGTGGCATTAATTCGDAATCC	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63948_Sspa_Oph2007_COI	BUNA13	1	BC63948_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	GAAGGCCTTCCGATGATC	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63948_Upin_Bot2011_COI	BUNA13	1	BC63948_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	CTTTAATTACAGCGTTTTTATTGTTGT	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63948_Vgib_Wil2025_COI	BUNA13	1	BC63948_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	CCCCTCTTTACAAGTTCTTTAGGGT	115.66214
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63949_Asen_Wil2025_COI	BUNA12	1	BC63949_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	GTTGTTGTTACAACACATGCTCTTTTG	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63949_Cjap_Sim2018_COI	BUNA12	1	BC63949_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63949_Esin_And2018_Cyb	BUNA12	1	BC63949_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA12	Cytb	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	ACCCCTCCTCATATCCAACCA	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63949_Mgig_Bot2012_COI	BUNA12	1	BC63949_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	TCTTATTCGTTGGAGACTTTATAACCCT	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63949_Mjap_Gib2022_COI	BUNA12	1	BC63949_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	AATTTAATTGTGACTAGGCATGGTCTG	115.66024
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63951_Aamu_Bax2006_COI	BUNA10	1	BC63951_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	GCACAACCGGGATCTTTACTTC	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63951_Pcan_Bot2011_IGS	BUNA10	1	BC63951_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA10	IGS	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	CGTAATCCTCAGTACTGGCTA	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63951_Pper_Dia2013_COI	BUNA10	1	BC63951_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	CTTAGTGGCATTAATTCGDAATCC	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63951_Sspa_Oph2007_COI	BUNA10	1	BC63951_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	GAAGGCCTTCCGATGATC	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63951_Upin_Bot2011_COI	BUNA10	1	BC63951_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	CTTTAATTACAGCGTTTTTATTGTTGT	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63951_Vgib_Wil2025_COI	BUNA10	1	BC63951_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	CCCCTCTTTACAAGTTCTTTAGGGT	115.66622
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63952_Asen_Wil2025_COI	BUNA9	1	BC63952_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	GTTGTTGTTACAACACATGCTCTTTTG	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63952_Cjap_Sim2018_COI	BUNA9	1	BC63952_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63952_Esin_And2018_Cyb	BUNA9	1	BC63952_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA9	Cytb	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	ACCCCTCCTCATATCCAACCA	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63952_Mgig_Bot2012_COI	BUNA9	1	BC63952_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	TCTTATTCGTTGGAGACTTTATAACCCT	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63952_Mjap_Gib2022_COI	BUNA9	1	BC63952_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	AATTTAATTGTGACTAGGCATGGTCTG	115.66476
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63953_Cmae_Bot2011_COI	BUNA8	1	BC63953_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	ATGAACAGTCTATCCTCCTTTAG	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63953_Hsan_Wil2021_COI	BUNA8	1	BC63953_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	CCTGGGCCGGTATAGTAGGT	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63953_Mros_Gib2022_COI	BUNA8	1	BC63953_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63953_Msal_Bot2012_COI	BUNA8	1	BC63953_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	GYTAGTTCCRATGATGTTAGCTG	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63953_Pvir_Dia2013_COI	BUNA8	1	BC63953_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	CTTAGTGGCATTAATTCGDAATCC	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63953_Rhar_Sim2018_COI	BUNA8	1	BC63953_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	CTGCTGCTATTGCTCATG	115.66291
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63955_Aamu_Bax2006_COI	BUNA6	1	BC63955_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	GCACAACCGGGATCTTTACTTC	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63955_Pcan_Bot2011_IGS	BUNA6	1	BC63955_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA6	IGS	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	CGTAATCCTCAGTACTGGCTA	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63955_Pper_Dia2013_COI	BUNA6	1	BC63955_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	CTTAGTGGCATTAATTCGDAATCC	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63955_Sspa_Oph2007_COI	BUNA6	1	BC63955_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	GAAGGCCTTCCGATGATC	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63955_Upin_Bot2011_COI	BUNA6	1	BC63955_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	CTTTAATTACAGCGTTTTTATTGTTGT	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63955_Vgib_Wil2025_COI	BUNA6	1	BC63955_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	CCCCTCTTTACAAGTTCTTTAGGGT	115.6689
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63956_Asen_Wil2025_COI	BUNA5	1	BC63956_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	GTTGTTGTTACAACACATGCTCTTTTG	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63956_Cjap_Sim2018_COI	BUNA5	1	BC63956_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63956_Esin_And2018_Cyb	BUNA5	1	BC63956_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA5	Cytb	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	ACCCCTCCTCATATCCAACCA	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63956_Mgig_Bot2012_COI	BUNA5	1	BC63956_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	TCTTATTCGTTGGAGACTTTATAACCCT	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63956_Mjap_Gib2022_COI	BUNA5	1	BC63956_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	AATTTAATTGTGACTAGGCATGGTCTG	115.66727
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63957_Cmae_Bot2011_COI	BUNA4	1	BC63957_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	ATGAACAGTCTATCCTCCTTTAG	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63957_Hsan_Wil2021_COI	BUNA4	1	BC63957_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	CCTGGGCCGGTATAGTAGGT	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63957_Mros_Gib2022_COI	BUNA4	1	BC63957_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63957_Msal_Bot2012_COI	BUNA4	1	BC63957_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	GYTAGTTCCRATGATGTTAGCTG	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63957_Pvir_Dia2013_COI	BUNA4	1	BC63957_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	CTTAGTGGCATTAATTCGDAATCC	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63957_Rhar_Sim2018_COI	BUNA4	1	BC63957_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	CTGCTGCTATTGCTCATG	115.66539
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63959_Aamu_Bax2006_COI	BUNA2	1	BC63959_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	GCACAACCGGGATCTTTACTTC	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63959_Pcan_Bot2011_IGS	BUNA2	1	BC63959_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA2	IGS	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	CGTAATCCTCAGTACTGGCTA	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63959_Pper_Dia2013_COI	BUNA2	1	BC63959_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	CTTAGTGGCATTAATTCGDAATCC	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63959_Sspa_Oph2007_COI	BUNA2	1	BC63959_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	GAAGGCCTTCCGATGATC	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63959_Upin_Bot2011_COI	BUNA2	1	BC63959_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	CTTTAATTACAGCGTTTTTATTGTTGT	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63959_Vgib_Wil2025_COI	BUNA2	1	BC63959_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	CCCCTCTTTACAAGTTCTTTAGGGT	115.66781
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63960_Cmae_Bot2011_COI	BUNA1	1	BC63960_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	ATGAACAGTCTATCCTCCTTTAG	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63960_Hsan_Wil2021_COI	BUNA1	1	BC63960_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	CCTGGGCCGGTATAGTAGGT	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63960_Mros_Gib2022_COI	BUNA1	1	BC63960_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63960_Msal_Bot2012_COI	BUNA1	1	BC63960_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	GYTAGTTCCRATGATGTTAGCTG	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63960_Pvir_Dia2013_COI	BUNA1	1	BC63960_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	CTTAGTGGCATTAATTCGDAATCC	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63960_Rhar_Sim2018_COI	BUNA1	1	BC63960_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	CTGCTGCTATTGCTCATG	115.66645
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63962_Aamu_Bax2006_COI	GERA14	1	BC63962_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	GCACAACCGGGATCTTTACTTC	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63962_Pcan_Bot2011_IGS	GERA14	1	BC63962_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA14	IGS	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	CGTAATCCTCAGTACTGGCTA	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63962_Pper_Dia2013_COI	GERA14	1	BC63962_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Pper_Dia2013_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	CTTAGTGGCATTAATTCGDAATCC	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63962_Sspa_Oph2007_COI	GERA14	1	BC63962_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	GAAGGCCTTCCGATGATC	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63962_Upin_Bot2011_COI	GERA14	1	BC63962_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Upin_Bot2011_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	CTTTAATTACAGCGTTTTTATTGTTGT	114.60049
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63963_Cjap_Sim2018_COI	GERA13	1	BC63963_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63963_Mgig_Bot2012_COI	GERA13	1	BC63963_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	TCTTATTCGTTGGAGACTTTATAACCCT	114.5987
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63964_Cmae_Bot2011_COI	GERA12	1	BC63964_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	ATGAACAGTCTATCCTCCTTTAG	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63964_Msal_Bot2012_COI	GERA12	1	BC63964_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Msal_Bot2012_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	GYTAGTTCCRATGATGTTAGCTG	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63964_Pvir_Dia2013_COI	GERA12	1	BC63964_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	CTTAGTGGCATTAATTCGDAATCC	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63964_Rhar_Sim2018_COI	GERA12	1	BC63964_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	CTGCTGCTATTGCTCATG	114.59739
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63966_Aamu_Bax2006_COI	GERA10	1	BC63966_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	GCACAACCGGGATCTTTACTTC	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63966_Pcan_Bot2011_IGS	GERA10	1	BC63966_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA10	IGS	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	CGTAATCCTCAGTACTGGCTA	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63966_Pper_Dia2013_COI	GERA10	1	BC63966_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Pper_Dia2013_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	CTTAGTGGCATTAATTCGDAATCC	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63966_Sspa_Oph2007_COI	GERA10	1	BC63966_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	GAAGGCCTTCCGATGATC	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63966_Upin_Bot2011_COI	GERA10	1	BC63966_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Upin_Bot2011_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	CTTTAATTACAGCGTTTTTATTGTTGT	114.60115
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63967_Cjap_Sim2018_COI	GERA9	1	BC63967_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63967_Mgig_Bot2012_COI	GERA9	1	BC63967_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	TCTTATTCGTTGGAGACTTTATAACCCT	114.5995
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63968_Cmae_Bot2011_COI	GERA8	1	BC63968_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	ATGAACAGTCTATCCTCCTTTAG	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63968_Msal_Bot2012_COI	GERA8	1	BC63968_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Msal_Bot2012_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	GYTAGTTCCRATGATGTTAGCTG	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63968_Pvir_Dia2013_COI	GERA8	1	BC63968_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	CTTAGTGGCATTAATTCGDAATCC	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63968_Rhar_Sim2018_COI	GERA8	1	BC63968_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	CTGCTGCTATTGCTCATG	114.59813
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63970_Cjap_Sim2018_COI	GERA6	1	BC63970_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63970_Mgig_Bot2012_COI	GERA6	1	BC63970_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	TCTTATTCGTTGGAGACTTTATAACCCT	114.6002
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63971_Cmae_Bot2011_COI	GERA5	1	BC63971_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	ATGAACAGTCTATCCTCCTTTAG	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63971_Msal_Bot2012_COI	GERA5	1	BC63971_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Msal_Bot2012_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	GYTAGTTCCRATGATGTTAGCTG	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63971_Pvir_Dia2013_COI	GERA5	1	BC63971_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	CTTAGTGGCATTAATTCGDAATCC	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63971_Rhar_Sim2018_COI	GERA5	1	BC63971_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	CTGCTGCTATTGCTCATG	114.59862
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63973_Aamu_Bax2006_COI	GERA3	1	BC63973_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	GCACAACCGGGATCTTTACTTC	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63973_Pcan_Bot2011_IGS	GERA3	1	BC63973_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA3	IGS	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	CGTAATCCTCAGTACTGGCTA	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63973_Pper_Dia2013_COI	GERA3	1	BC63973_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Pper_Dia2013_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	CTTAGTGGCATTAATTCGDAATCC	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63973_Sspa_Oph2007_COI	GERA3	1	BC63973_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	GAAGGCCTTCCGATGATC	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63973_Upin_Bot2011_COI	GERA3	1	BC63973_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Upin_Bot2011_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	CTTTAATTACAGCGTTTTTATTGTTGT	114.59549
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63974_Cjap_Sim2018_COI	GERA2	1	BC63974_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63974_Mgig_Bot2012_COI	GERA2	1	BC63974_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	TCTTATTCGTTGGAGACTTTATAACCCT	114.59598
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63975_Cmae_Bot2011_COI	GERA1	1	BC63975_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	ATGAACAGTCTATCCTCCTTTAG	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63975_Msal_Bot2012_COI	GERA1	1	BC63975_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Msal_Bot2012_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	GYTAGTTCCRATGATGTTAGCTG	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63975_Pvir_Dia2013_COI	GERA1	1	BC63975_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	CTTAGTGGCATTAATTCGDAATCC	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63975_Rhar_Sim2018_COI	GERA1	1	BC63975_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	CTGCTGCTATTGCTCATG	114.59449
BC64564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81059	WGS84	BC64564	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64564_Cjap_Sim2018_COI	ADEAPC-60	1	BC64564_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64564	Sample	BC64564	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-60	COI	45	BC64564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-60	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54369
BC64564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81059	WGS84	BC64564	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.68	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64564_Mgig_Bot2012_COI	ADEAPC-60	1	BC64564_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64564	Sample	BC64564	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-60	COI	45	BC64564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-60	TCTTATTCGTTGGAGACTTTATAACCCT	138.54369
BC64565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8123	WGS84	BC64565	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64565_Cmae_Bot2011_COI	ADEAPC-59	1	BC64565_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64565	Sample	BC64565	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-59	COI	45	BC64565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-59	ATGAACAGTCTATCCTCCTTTAG	138.53897
BC64567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81246	WGS84	BC64567	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64567_Aamu_Bax2006_COI	ADEAPC-57	1	BC64567_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64567	Sample	BC64567	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-57	COI	45	BC64567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-57	GCACAACCGGGATCTTTACTTC	138.52837
BC64567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81246	WGS84	BC64567	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64567_Pcan_Bot2011_IGS	ADEAPC-57	1	BC64567_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64567	Sample	BC64567	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-57	IGS	45	BC64567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-57	CGTAATCCTCAGTACTGGCTA	138.52837
BC64567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81246	WGS84	BC64567	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.633	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64567_Sspa_Oph2007_COI	ADEAPC-57	1	BC64567_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64567	Sample	BC64567	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-57	COI	45	BC64567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-57	GAAGGCCTTCCGATGATC	138.52837
BC64567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81246	WGS84	BC64567	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64567_Upin_Bot2011_COI	ADEAPC-57	1	BC64567_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64567	Sample	BC64567	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-57	COI	45	BC64567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-57	CTTTAATTACAGCGTTTTTATTGTTGT	138.52837
BC64568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81501	WGS84	BC64568	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64568_Cjap_Sim2018_COI	ADEAPC-56	1	BC64568_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64568	Sample	BC64568	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-56	COI	45	BC64568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-56	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51475
BC64568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81501	WGS84	BC64568	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.635	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64568_Mgig_Bot2012_COI	ADEAPC-56	1	BC64568_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64568	Sample	BC64568	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-56	COI	45	BC64568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-56	TCTTATTCGTTGGAGACTTTATAACCCT	138.51475
BC64569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81915	WGS84	BC64569	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64569_Cmae_Bot2011_COI	ADEAPC-55	1	BC64569_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64569	Sample	BC64569	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-55	COI	45	BC64569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-55	ATGAACAGTCTATCCTCCTTTAG	138.51385
BC64570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83039	WGS84	BC64570	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64570_Aamu_Bax2006_COI	ADEAPC-54	1	BC64570_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64570	Sample	BC64570	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-54	COI	45	BC64570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-54	GCACAACCGGGATCTTTACTTC	138.51097
BC64570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83039	WGS84	BC64570	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64570_Pcan_Bot2011_IGS	ADEAPC-54	1	BC64570_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64570	Sample	BC64570	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-54	IGS	45	BC64570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-54	CGTAATCCTCAGTACTGGCTA	138.51097
BC64570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83039	WGS84	BC64570	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.665	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64570_Sspa_Oph2007_COI	ADEAPC-54	1	BC64570_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64570	Sample	BC64570	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-54	COI	45	BC64570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-54	GAAGGCCTTCCGATGATC	138.51097
BC64570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83039	WGS84	BC64570	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64570_Upin_Bot2011_COI	ADEAPC-54	1	BC64570_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64570	Sample	BC64570	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-54	COI	45	BC64570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-54	CTTTAATTACAGCGTTTTTATTGTTGT	138.51097
BC64571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83047	WGS84	BC64571	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64571_Cjap_Sim2018_COI	ADEAPC-53	1	BC64571_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64571	Sample	BC64571	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-53	COI	45	BC64571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-53	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51076
BC64571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83047	WGS84	BC64571	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.937	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64571_Mgig_Bot2012_COI	ADEAPC-53	1	BC64571_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64571	Sample	BC64571	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-53	COI	45	BC64571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-53	TCTTATTCGTTGGAGACTTTATAACCCT	138.51076
BC64572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83034	WGS84	BC64572	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64572_Cmae_Bot2011_COI	ADEAPC-52	1	BC64572_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64572	Sample	BC64572	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-52	COI	45	BC64572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-52	ATGAACAGTCTATCCTCCTTTAG	138.51084
BC64574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83719	WGS84	BC64574	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64574_Aamu_Bax2006_COI	ADEAPC-50	1	BC64574_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64574	Sample	BC64574	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-50	COI	45	BC64574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-50	GCACAACCGGGATCTTTACTTC	138.5105
BC64574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83719	WGS84	BC64574	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64574_Pcan_Bot2011_IGS	ADEAPC-50	1	BC64574_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64574	Sample	BC64574	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-50	IGS	45	BC64574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-50	CGTAATCCTCAGTACTGGCTA	138.5105
BC64574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83719	WGS84	BC64574	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.93	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64574_Sspa_Oph2007_COI	ADEAPC-50	1	BC64574_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64574	Sample	BC64574	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-50	COI	45	BC64574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-50	GAAGGCCTTCCGATGATC	138.5105
BC64574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83719	WGS84	BC64574	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64574_Upin_Bot2011_COI	ADEAPC-50	1	BC64574_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64574	Sample	BC64574	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-50	COI	45	BC64574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-50	CTTTAATTACAGCGTTTTTATTGTTGT	138.5105
BC64575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83738	WGS84	BC64575	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64575_Cjap_Sim2018_COI	ADEAPC-49	1	BC64575_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64575	Sample	BC64575	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-49	COI	45	BC64575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-49	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51065
BC64575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83738	WGS84	BC64575	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.567	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64575_Mgig_Bot2012_COI	ADEAPC-49	1	BC64575_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64575	Sample	BC64575	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-49	COI	45	BC64575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-49	TCTTATTCGTTGGAGACTTTATAACCCT	138.51065
BC64576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83724	WGS84	BC64576	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64576_Cmae_Bot2011_COI	ADEAPC-48	1	BC64576_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64576	Sample	BC64576	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-48	COI	45	BC64576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-48	ATGAACAGTCTATCCTCCTTTAG	138.51052
BC64578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8079	WGS84	BC64578	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64578_Aamu_Bax2006_COI	ADEAPC-46	1	BC64578_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64578	Sample	BC64578	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-46	COI	45	BC64578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-46	GCACAACCGGGATCTTTACTTC	138.54826
BC64578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8079	WGS84	BC64578	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64578_Pcan_Bot2011_IGS	ADEAPC-46	1	BC64578_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64578	Sample	BC64578	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-46	IGS	45	BC64578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-46	CGTAATCCTCAGTACTGGCTA	138.54826
BC64578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8079	WGS84	BC64578	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.364	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64578_Sspa_Oph2007_COI	ADEAPC-46	1	BC64578_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64578	Sample	BC64578	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-46	COI	45	BC64578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-46	GAAGGCCTTCCGATGATC	138.54826
BC64578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8079	WGS84	BC64578	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64578_Upin_Bot2011_COI	ADEAPC-46	1	BC64578_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64578	Sample	BC64578	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-46	COI	45	BC64578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-46	CTTTAATTACAGCGTTTTTATTGTTGT	138.54826
BC64579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80372	WGS84	BC64579	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64579_Cjap_Sim2018_COI	ADEAPC-45	1	BC64579_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64579	Sample	BC64579	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-45	COI	45	BC64579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-45	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54048
BC64579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80372	WGS84	BC64579	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.322	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64579_Mgig_Bot2012_COI	ADEAPC-45	1	BC64579_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64579	Sample	BC64579	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-45	COI	45	BC64579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-45	TCTTATTCGTTGGAGACTTTATAACCCT	138.54048
BC64581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80329	WGS84	BC64581	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64581_Aamu_Bax2006_COI	ADEAPC-43	1	BC64581_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64581	Sample	BC64581	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-43	COI	45	BC64581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-43	GCACAACCGGGATCTTTACTTC	138.54832
BC64581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80329	WGS84	BC64581	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64581_Pcan_Bot2011_IGS	ADEAPC-43	1	BC64581_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64581	Sample	BC64581	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-43	IGS	45	BC64581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-43	CGTAATCCTCAGTACTGGCTA	138.54832
BC64581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80329	WGS84	BC64581	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.085	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64581_Sspa_Oph2007_COI	ADEAPC-43	1	BC64581_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64581	Sample	BC64581	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-43	COI	45	BC64581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-43	GAAGGCCTTCCGATGATC	138.54832
BC64581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80329	WGS84	BC64581	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64581_Upin_Bot2011_COI	ADEAPC-43	1	BC64581_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64581	Sample	BC64581	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-43	COI	45	BC64581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-43	CTTTAATTACAGCGTTTTTATTGTTGT	138.54832
BC64582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8005	WGS84	BC64582	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64582_Cjap_Sim2018_COI	ADEAPC-42	1	BC64582_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64582	Sample	BC64582	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-42	COI	45	BC64582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-42	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54783
BC64582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8005	WGS84	BC64582	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.481	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64582_Mgig_Bot2012_COI	ADEAPC-42	1	BC64582_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64582	Sample	BC64582	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-42	COI	45	BC64582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-42	TCTTATTCGTTGGAGACTTTATAACCCT	138.54783
BC64583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79895	WGS84	BC64583	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64583_Cmae_Bot2011_COI	ADEAPC-41	1	BC64583_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64583	Sample	BC64583	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-41	COI	45	BC64583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-41	ATGAACAGTCTATCCTCCTTTAG	138.54817
BC64585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79078	WGS84	BC64585	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64585_Aamu_Bax2006_COI	ADEAPC-39	1	BC64585_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64585	Sample	BC64585	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-39	COI	45	BC64585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-39	GCACAACCGGGATCTTTACTTC	138.54425
BC64585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79078	WGS84	BC64585	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64585_Pcan_Bot2011_IGS	ADEAPC-39	1	BC64585_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64585	Sample	BC64585	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-39	IGS	45	BC64585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-39	CGTAATCCTCAGTACTGGCTA	138.54425
BC64585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79078	WGS84	BC64585	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.546	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64585_Sspa_Oph2007_COI	ADEAPC-39	1	BC64585_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64585	Sample	BC64585	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-39	COI	45	BC64585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-39	GAAGGCCTTCCGATGATC	138.54425
BC64585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79078	WGS84	BC64585	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64585_Upin_Bot2011_COI	ADEAPC-39	1	BC64585_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64585	Sample	BC64585	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-39	COI	45	BC64585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-39	CTTTAATTACAGCGTTTTTATTGTTGT	138.54425
BC64586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78712	WGS84	BC64586	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64586_Cjap_Sim2018_COI	ADEAPC-38	1	BC64586_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64586	Sample	BC64586	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-38	COI	45	BC64586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-38	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54309
BC64586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78712	WGS84	BC64586	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.483	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64586_Mgig_Bot2012_COI	ADEAPC-38	1	BC64586_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64586	Sample	BC64586	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-38	COI	45	BC64586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-38	TCTTATTCGTTGGAGACTTTATAACCCT	138.54309
BC64587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78384	WGS84	BC64587	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64587_Cmae_Bot2011_COI	ADEAPC-37	1	BC64587_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64587	Sample	BC64587	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-37	COI	45	BC64587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-37	ATGAACAGTCTATCCTCCTTTAG	138.54032
BC64589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77762	WGS84	BC64589	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64589_Aamu_Bax2006_COI	ADEAPC-35	1	BC64589_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64589	Sample	BC64589	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-35	COI	45	BC64589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-35	GCACAACCGGGATCTTTACTTC	138.53481
BC64589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77762	WGS84	BC64589	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64589_Pcan_Bot2011_IGS	ADEAPC-35	1	BC64589_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64589	Sample	BC64589	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-35	IGS	45	BC64589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-35	CGTAATCCTCAGTACTGGCTA	138.53481
BC64589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77762	WGS84	BC64589	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.389	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64589_Sspa_Oph2007_COI	ADEAPC-35	1	BC64589_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64589	Sample	BC64589	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-35	COI	45	BC64589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-35	GAAGGCCTTCCGATGATC	138.53481
BC64589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77762	WGS84	BC64589	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64589_Upin_Bot2011_COI	ADEAPC-35	1	BC64589_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64589	Sample	BC64589	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-35	COI	45	BC64589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-35	CTTTAATTACAGCGTTTTTATTGTTGT	138.53481
BC64590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77469	WGS84	BC64590	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64590_Cmae_Bot2011_COI	ADEAPC-34	1	BC64590_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64590	Sample	BC64590	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-34	COI	45	BC64590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-34	ATGAACAGTCTATCCTCCTTTAG	138.53169
BC64592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76757	WGS84	BC64592	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64592_Aamu_Bax2006_COI	ADEAPC-32	1	BC64592_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64592	Sample	BC64592	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-32	COI	45	BC64592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-32	GCACAACCGGGATCTTTACTTC	138.5283
BC64592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76757	WGS84	BC64592	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64592_Pcan_Bot2011_IGS	ADEAPC-32	1	BC64592_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64592	Sample	BC64592	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-32	IGS	45	BC64592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-32	CGTAATCCTCAGTACTGGCTA	138.5283
BC64592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76757	WGS84	BC64592	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	44.225	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64592_Sspa_Oph2007_COI	ADEAPC-32	1	BC64592_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64592	Sample	BC64592	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-32	COI	45	BC64592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-32	GAAGGCCTTCCGATGATC	138.5283
BC64592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76757	WGS84	BC64592	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64592_Upin_Bot2011_COI	ADEAPC-32	1	BC64592_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64592	Sample	BC64592	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-32	COI	45	BC64592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-32	CTTTAATTACAGCGTTTTTATTGTTGT	138.5283
BC64593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76424	WGS84	BC64593	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64593_Cjap_Sim2018_COI	ADEAPC-31	1	BC64593_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64593	Sample	BC64593	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-31	COI	45	BC64593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-31	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52591
BC64593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76424	WGS84	BC64593	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.97	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64593_Mgig_Bot2012_COI	ADEAPC-31	1	BC64593_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64593	Sample	BC64593	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-31	COI	45	BC64593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-31	TCTTATTCGTTGGAGACTTTATAACCCT	138.52591
BC64594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76096	WGS84	BC64594	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64594_Cmae_Bot2011_COI	ADEAPC-30	1	BC64594_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64594	Sample	BC64594	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-30	COI	45	BC64594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-30	ATGAACAGTCTATCCTCCTTTAG	138.52349
BC64596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81098	WGS84	BC64596	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64596_Aamu_Bax2006_COI	ADEAPC-28	1	BC64596_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64596	Sample	BC64596	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-28	COI	45	BC64596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-28	GCACAACCGGGATCTTTACTTC	138.52321
BC64596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81098	WGS84	BC64596	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64596_Pcan_Bot2011_IGS	ADEAPC-28	1	BC64596_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64596	Sample	BC64596	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-28	IGS	45	BC64596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-28	CGTAATCCTCAGTACTGGCTA	138.52321
BC64596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81098	WGS84	BC64596	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.506	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64596_Sspa_Oph2007_COI	ADEAPC-28	1	BC64596_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64596	Sample	BC64596	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-28	COI	45	BC64596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-28	GAAGGCCTTCCGATGATC	138.52321
BC64596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81098	WGS84	BC64596	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64596_Upin_Bot2011_COI	ADEAPC-28	1	BC64596_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64596	Sample	BC64596	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-28	COI	45	BC64596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-28	CTTTAATTACAGCGTTTTTATTGTTGT	138.52321
BC64597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81029	WGS84	BC64597	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64597_Cjap_Sim2018_COI	ADEAPC-27	1	BC64597_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64597	Sample	BC64597	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-27	COI	45	BC64597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-27	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52168
BC64597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81029	WGS84	BC64597	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.912	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64597_Mgig_Bot2012_COI	ADEAPC-27	1	BC64597_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64597	Sample	BC64597	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-27	COI	45	BC64597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-27	TCTTATTCGTTGGAGACTTTATAACCCT	138.52168
BC64598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80908	WGS84	BC64598	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64598_Cmae_Bot2011_COI	ADEAPC-26	1	BC64598_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64598	Sample	BC64598	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-26	COI	45	BC64598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-26	ATGAACAGTCTATCCTCCTTTAG	138.51913
BC64600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80373	WGS84	BC64600	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64600_Aamu_Bax2006_COI	ADEAPC-24	1	BC64600_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64600	Sample	BC64600	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-24	COI	45	BC64600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-24	GCACAACCGGGATCTTTACTTC	138.51423
BC64600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80373	WGS84	BC64600	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64600_Pcan_Bot2011_IGS	ADEAPC-24	1	BC64600_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64600	Sample	BC64600	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-24	IGS	45	BC64600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-24	CGTAATCCTCAGTACTGGCTA	138.51423
BC64600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80373	WGS84	BC64600	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.378	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64600_Sspa_Oph2007_COI	ADEAPC-24	1	BC64600_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64600	Sample	BC64600	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-24	COI	45	BC64600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-24	GAAGGCCTTCCGATGATC	138.51423
BC64600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80373	WGS84	BC64600	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64600_Upin_Bot2011_COI	ADEAPC-24	1	BC64600_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64600	Sample	BC64600	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-24	COI	45	BC64600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-24	CTTTAATTACAGCGTTTTTATTGTTGT	138.51423
BC64601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80038	WGS84	BC64601	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64601_Cjap_Sim2018_COI	ADEAPC-23	1	BC64601_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64601	Sample	BC64601	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-23	COI	45	BC64601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-23	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51358
BC64601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80038	WGS84	BC64601	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.488	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64601_Mgig_Bot2012_COI	ADEAPC-23	1	BC64601_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64601	Sample	BC64601	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-23	COI	45	BC64601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-23	TCTTATTCGTTGGAGACTTTATAACCCT	138.51358
BC64602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79792	WGS84	BC64602	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64602_Cmae_Bot2011_COI	ADEAPC-22	1	BC64602_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64602	Sample	BC64602	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-22	COI	45	BC64602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-22	ATGAACAGTCTATCCTCCTTTAG	138.51337
BC64604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79054	WGS84	BC64604	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64604_Aamu_Bax2006_COI	ADEAPC-20	1	BC64604_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64604	Sample	BC64604	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-20	COI	45	BC64604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-20	GCACAACCGGGATCTTTACTTC	138.51486
BC64604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79054	WGS84	BC64604	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64604_Pcan_Bot2011_IGS	ADEAPC-20	1	BC64604_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64604	Sample	BC64604	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-20	IGS	45	BC64604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-20	CGTAATCCTCAGTACTGGCTA	138.51486
BC64604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79054	WGS84	BC64604	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.894	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64604_Sspa_Oph2007_COI	ADEAPC-20	1	BC64604_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64604	Sample	BC64604	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-20	COI	45	BC64604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-20	GAAGGCCTTCCGATGATC	138.51486
BC64604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79054	WGS84	BC64604	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64604_Upin_Bot2011_COI	ADEAPC-20	1	BC64604_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64604	Sample	BC64604	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-20	COI	45	BC64604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-20	CTTTAATTACAGCGTTTTTATTGTTGT	138.51486
BC64605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78832	WGS84	BC64605	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64605_Cjap_Sim2018_COI	ADEAPC-19	1	BC64605_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64605	Sample	BC64605	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-19	COI	45	BC64605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51547
BC64605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78832	WGS84	BC64605	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.039	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64605_Mgig_Bot2012_COI	ADEAPC-19	1	BC64605_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64605	Sample	BC64605	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-19	COI	45	BC64605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.51547
BC64606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78608	WGS84	BC64606	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64606_Cmae_Bot2011_COI	ADEAPC-18	1	BC64606_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64606	Sample	BC64606	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-18	COI	45	BC64606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-18	ATGAACAGTCTATCCTCCTTTAG	138.51602
BC64608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78074	WGS84	BC64608	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64608_Aamu_Bax2006_COI	ADEAPC-16	1	BC64608_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64608	Sample	BC64608	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-16	COI	45	BC64608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-16	GCACAACCGGGATCTTTACTTC	138.51766
BC64608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78074	WGS84	BC64608	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64608_Pcan_Bot2011_IGS	ADEAPC-16	1	BC64608_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64608	Sample	BC64608	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-16	IGS	45	BC64608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-16	CGTAATCCTCAGTACTGGCTA	138.51766
BC64608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78074	WGS84	BC64608	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.524	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64608_Sspa_Oph2007_COI	ADEAPC-16	1	BC64608_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64608	Sample	BC64608	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-16	COI	45	BC64608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-16	GAAGGCCTTCCGATGATC	138.51766
BC64608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78074	WGS84	BC64608	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64608_Upin_Bot2011_COI	ADEAPC-16	1	BC64608_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64608	Sample	BC64608	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-16	COI	45	BC64608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.51766
BC64609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.778	WGS84	BC64609	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64609_Cjap_Sim2018_COI	ADEAPC-15	1	BC64609_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64609	Sample	BC64609	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-15	COI	45	BC64609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51913
BC64609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.778	WGS84	BC64609	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.62	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64609_Mgig_Bot2012_COI	ADEAPC-15	1	BC64609_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64609	Sample	BC64609	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-15	COI	45	BC64609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.51913
BC64611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76972	WGS84	BC64611	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64611_Aamu_Bax2006_COI	ADEAPC-13	1	BC64611_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64611	Sample	BC64611	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-13	COI	45	BC64611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-13	GCACAACCGGGATCTTTACTTC	138.51927
BC64611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76972	WGS84	BC64611	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64611_Pcan_Bot2011_IGS	ADEAPC-13	1	BC64611_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64611	Sample	BC64611	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-13	IGS	45	BC64611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-13	CGTAATCCTCAGTACTGGCTA	138.51927
BC64611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76972	WGS84	BC64611	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.733	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64611_Sspa_Oph2007_COI	ADEAPC-13	1	BC64611_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64611	Sample	BC64611	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-13	COI	45	BC64611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-13	GAAGGCCTTCCGATGATC	138.51927
BC64611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76972	WGS84	BC64611	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64611_Upin_Bot2011_COI	ADEAPC-13	1	BC64611_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64611	Sample	BC64611	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-13	COI	45	BC64611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.51927
BC64612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76536	WGS84	BC64612	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64612_Cjap_Sim2018_COI	ADEAPC-12	1	BC64612_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64612	Sample	BC64612	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-12	COI	45	BC64612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51727
BC64612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76536	WGS84	BC64612	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.544	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64612_Mgig_Bot2012_COI	ADEAPC-12	1	BC64612_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64612	Sample	BC64612	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-12	COI	45	BC64612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.51727
BC64613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76148	WGS84	BC64613	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64613_Cmae_Bot2011_COI	ADEAPC-11	1	BC64613_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64613	Sample	BC64613	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-11	COI	45	BC64613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-11	ATGAACAGTCTATCCTCCTTTAG	138.51153
BC64615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76007	WGS84	BC64615	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64615_Aamu_Bax2006_COI	ADEAPC-09	1	BC64615_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64615	Sample	BC64615	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-09	COI	45	BC64615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-09	GCACAACCGGGATCTTTACTTC	138.50254
BC64615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76007	WGS84	BC64615	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64615_Pcan_Bot2011_IGS	ADEAPC-09	1	BC64615_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64615	Sample	BC64615	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-09	IGS	45	BC64615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-09	CGTAATCCTCAGTACTGGCTA	138.50254
BC64615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76007	WGS84	BC64615	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.119	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64615_Sspa_Oph2007_COI	ADEAPC-09	1	BC64615_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64615	Sample	BC64615	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-09	COI	45	BC64615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-09	GAAGGCCTTCCGATGATC	138.50254
BC64615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76007	WGS84	BC64615	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64615_Upin_Bot2011_COI	ADEAPC-09	1	BC64615_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64615	Sample	BC64615	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-09	COI	45	BC64615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.50254
BC64616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76234	WGS84	BC64616	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64616_Cjap_Sim2018_COI	ADEAPC-08	1	BC64616_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64616	Sample	BC64616	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-08	COI	45	BC64616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49388
BC64616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76234	WGS84	BC64616	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.34	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64616_Mgig_Bot2012_COI	ADEAPC-08	1	BC64616_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64616	Sample	BC64616	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-08	COI	45	BC64616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.49388
BC64617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76304	WGS84	BC64617	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64617_Cmae_Bot2011_COI	ADEAPC-07	1	BC64617_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64617	Sample	BC64617	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-07	COI	45	BC64617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-07	ATGAACAGTCTATCCTCCTTTAG	138.49257
BC64619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76454	WGS84	BC64619	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64619_Aamu_Bax2006_COI	ADEAPC-05	1	BC64619_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64619	Sample	BC64619	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-05	COI	45	BC64619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-05	GCACAACCGGGATCTTTACTTC	138.49035
BC64619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76454	WGS84	BC64619	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64619_Pcan_Bot2011_IGS	ADEAPC-05	1	BC64619_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64619	Sample	BC64619	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-05	IGS	45	BC64619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-05	CGTAATCCTCAGTACTGGCTA	138.49035
BC64619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76454	WGS84	BC64619	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.092	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64619_Sspa_Oph2007_COI	ADEAPC-05	1	BC64619_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64619	Sample	BC64619	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-05	COI	45	BC64619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-05	GAAGGCCTTCCGATGATC	138.49035
BC64619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76454	WGS84	BC64619	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64619_Upin_Bot2011_COI	ADEAPC-05	1	BC64619_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64619	Sample	BC64619	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-05	COI	45	BC64619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.49035
BC64620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76871	WGS84	BC64620	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.344	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64620_Cmae_Bot2011_COI	ADEAPC-04	1	BC64620_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64620	Sample	BC64620	targeted	Present	Sample	Cmae_Bot2011_COI	ADEAPC-04	COI	45	BC64620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-04	ATGAACAGTCTATCCTCCTTTAG	138.48329
BC64622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.7704	WGS84	BC64622	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64622_Aamu_Bax2006_COI	ADEAPC-02	1	BC64622_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64622	Sample	BC64622	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-02	COI	45	BC64622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-02	GCACAACCGGGATCTTTACTTC	138.48253
BC64622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.7704	WGS84	BC64622	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64622_Pcan_Bot2011_IGS	ADEAPC-02	1	BC64622_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64622	Sample	BC64622	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-02	IGS	45	BC64622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-02	CGTAATCCTCAGTACTGGCTA	138.48253
BC64622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.7704	WGS84	BC64622	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.583	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64622_Sspa_Oph2007_COI	ADEAPC-02	1	BC64622_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64622	Sample	BC64622	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-02	COI	45	BC64622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-02	GAAGGCCTTCCGATGATC	138.48253
BC64622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.7704	WGS84	BC64622	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64622_Upin_Bot2011_COI	ADEAPC-02	1	BC64622_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64622	Sample	BC64622	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-02	COI	45	BC64622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.48253
BC64623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77103	WGS84	BC64623	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64623_Cjap_Sim2018_COI	ADEAPC-01	1	BC64623_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64623	Sample	BC64623	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-01	COI	45	BC64623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48203
BC64623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77103	WGS84	BC64623	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.132	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64623_Mgig_Bot2012_COI	ADEAPC-01	1	BC64623_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64623	Sample	BC64623	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-01	COI	45	BC64623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.48203
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC76569_Cjap_Sim2018_COI	GERB1	1	BC76569_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC76569_Mgig_Bot2012_COI	GERB1	1	BC76569_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	TCTTATTCGTTGGAGACTTTATAACCCT	114.59414
BC77251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84457	WGS84	BC77251	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77251_Cmae_Bot2011_COI	S5-04	1	BC77251_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77251	Sample	BC77251	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-04	COI	45	BC77251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-04	ATGAACAGTCTATCCTCCTTTAG	138.49741
BC77253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78389	WGS84	BC77253	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77253_Aamu_Bax2006_COI	S5-14	1	BC77253_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77253	Sample	BC77253	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-14	COI	45	BC77253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-14	GCACAACCGGGATCTTTACTTC	138.51561
BC77253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78389	WGS84	BC77253	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77253_Pcan_Bot2011_IGS	S5-14	1	BC77253_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77253	Sample	BC77253	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-14	IGS	45	BC77253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-14	CGTAATCCTCAGTACTGGCTA	138.51561
BC77253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78389	WGS84	BC77253	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.863	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77253_Sspa_Oph2007_COI	S5-14	1	BC77253_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77253	Sample	BC77253	targeted	Present	Sample	Sspa_Oph2007_COI	S5-14	COI	45	BC77253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-14	GAAGGCCTTCCGATGATC	138.51561
BC77253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78389	WGS84	BC77253	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77253_Upin_Bot2011_COI	S5-14	1	BC77253_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77253	Sample	BC77253	targeted	Absent	Sample	Upin_Bot2011_COI	S5-14	COI	45	BC77253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.51561
BC77254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77376	WGS84	BC77254	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77254_Cjap_Sim2018_COI	S5-19	1	BC77254_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77254	Sample	BC77254	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-19	COI	45	BC77254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48542
BC77254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77376	WGS84	BC77254	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.998	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77254_Mgig_Bot2012_COI	S5-19	1	BC77254_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77254	Sample	BC77254	targeted	Present	Sample	Mgig_Bot2012_COI	S5-19	COI	45	BC77254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.48542
BC77255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81058	WGS84	BC77255	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77255_Cmae_Bot2011_COI	S5-23	1	BC77255_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77255	Sample	BC77255	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-23	COI	45	BC77255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-23	ATGAACAGTCTATCCTCCTTTAG	138.52126
BC77257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83669	WGS84	BC77257	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77257_Aamu_Bax2006_COI	S6-09	1	BC77257_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77257	Sample	BC77257	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-09	COI	45	BC77257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-09	GCACAACCGGGATCTTTACTTC	138.5098
BC77257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83669	WGS84	BC77257	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77257_Pcan_Bot2011_IGS	S6-09	1	BC77257_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77257	Sample	BC77257	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-09	IGS	45	BC77257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-09	CGTAATCCTCAGTACTGGCTA	138.5098
BC77257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83669	WGS84	BC77257	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77257_Sspa_Oph2007_COI	S6-09	1	BC77257_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77257	Sample	BC77257	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-09	COI	45	BC77257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-09	GAAGGCCTTCCGATGATC	138.5098
BC77257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83669	WGS84	BC77257	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77257_Upin_Bot2011_COI	S6-09	1	BC77257_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77257	Sample	BC77257	targeted	Absent	Sample	Upin_Bot2011_COI	S6-09	COI	45	BC77257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.5098
BC77258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78399	WGS84	BC77258	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77258_Cjap_Sim2018_COI	S6-14	1	BC77258_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77258	Sample	BC77258	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-14	COI	45	BC77258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51578
BC77258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78399	WGS84	BC77258	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77258_Mgig_Bot2012_COI	S6-14	1	BC77258_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77258	Sample	BC77258	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-14	COI	45	BC77258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.51578
BC77259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77244	WGS84	BC77259	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77259_Cmae_Bot2011_COI	S6-19	1	BC77259_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77259	Sample	BC77259	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-19	COI	45	BC77259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-19	ATGAACAGTCTATCCTCCTTTAG	138.48557
BC77260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81082	WGS84	BC77260	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77260_Aamu_Bax2006_COI	S6-23	1	BC77260_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77260	Sample	BC77260	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-23	COI	45	BC77260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-23	GCACAACCGGGATCTTTACTTC	138.52258
BC77260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81082	WGS84	BC77260	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77260_Pcan_Bot2011_IGS	S6-23	1	BC77260_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77260	Sample	BC77260	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-23	IGS	45	BC77260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-23	CGTAATCCTCAGTACTGGCTA	138.52258
BC77260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81082	WGS84	BC77260	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77260_Sspa_Oph2007_COI	S6-23	1	BC77260_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77260	Sample	BC77260	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-23	COI	45	BC77260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-23	GAAGGCCTTCCGATGATC	138.52258
BC77260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81082	WGS84	BC77260	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77260_Upin_Bot2011_COI	S6-23	1	BC77260_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77260	Sample	BC77260	targeted	Absent	Sample	Upin_Bot2011_COI	S6-23	COI	45	BC77260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-23	CTTTAATTACAGCGTTTTTATTGTTGT	138.52258
BC77261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84489	WGS84	BC77261	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77261_Cjap_Sim2018_COI	S7-04	1	BC77261_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77261	Sample	BC77261	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-04	COI	45	BC77261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49712
BC77261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84489	WGS84	BC77261	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77261_Mgig_Bot2012_COI	S7-04	1	BC77261_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77261	Sample	BC77261	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-04	COI	45	BC77261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49712
BC77262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83653	WGS84	BC77262	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77262_Cmae_Bot2011_COI	S7-09	1	BC77262_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77262	Sample	BC77262	targeted	Absent	Sample	Cmae_Bot2011_COI	S7-09	COI	45	BC77262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-09	ATGAACAGTCTATCCTCCTTTAG	138.50914
BC77264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77342	WGS84	BC77264	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77264_Aamu_Bax2006_COI	S7-19	1	BC77264_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77264	Sample	BC77264	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-19	COI	45	BC77264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-19	GCACAACCGGGATCTTTACTTC	138.48496
BC77264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77342	WGS84	BC77264	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77264_Pcan_Bot2011_IGS	S7-19	1	BC77264_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77264	Sample	BC77264	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-19	IGS	45	BC77264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-19	CGTAATCCTCAGTACTGGCTA	138.48496
BC77264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77342	WGS84	BC77264	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.005	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77264_Sspa_Oph2007_COI	S7-19	1	BC77264_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77264	Sample	BC77264	targeted	Present	Sample	Sspa_Oph2007_COI	S7-19	COI	45	BC77264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-19	GAAGGCCTTCCGATGATC	138.48496
BC77264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77342	WGS84	BC77264	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77264_Upin_Bot2011_COI	S7-19	1	BC77264_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77264	Sample	BC77264	targeted	Absent	Sample	Upin_Bot2011_COI	S7-19	COI	45	BC77264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.48496
BC77265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81053	WGS84	BC77265	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77265_Cjap_Sim2018_COI	S7-23	1	BC77265_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77265	Sample	BC77265	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-23	COI	45	BC77265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-23	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52131
BC77265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81053	WGS84	BC77265	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77265_Mgig_Bot2012_COI	S7-23	1	BC77265_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77265	Sample	BC77265	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-23	COI	45	BC77265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-23	TCTTATTCGTTGGAGACTTTATAACCCT	138.52131
BC77266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81019	WGS84	BC77266	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.466	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77266_Cmae_Bot2011_COI	S8-04	1	BC77266_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77266	Sample	BC77266	targeted	Present	Sample	Cmae_Bot2011_COI	S8-04	COI	45	BC77266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-04	ATGAACAGTCTATCCTCCTTTAG	138.52036
BC77268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7737	WGS84	BC77268	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77268_Aamu_Bax2006_COI	S8-14	1	BC77268_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77268	Sample	BC77268	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-14	COI	45	BC77268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-14	GCACAACCGGGATCTTTACTTC	138.48409
BC77268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7737	WGS84	BC77268	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77268_Pcan_Bot2011_IGS	S8-14	1	BC77268_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77268	Sample	BC77268	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-14	IGS	45	BC77268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-14	CGTAATCCTCAGTACTGGCTA	138.48409
BC77268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7737	WGS84	BC77268	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.847	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77268_Sspa_Oph2007_COI	S8-14	1	BC77268_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77268	Sample	BC77268	targeted	Present	Sample	Sspa_Oph2007_COI	S8-14	COI	45	BC77268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-14	GAAGGCCTTCCGATGATC	138.48409
BC77268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7737	WGS84	BC77268	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77268_Upin_Bot2011_COI	S8-14	1	BC77268_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77268	Sample	BC77268	targeted	Absent	Sample	Upin_Bot2011_COI	S8-14	COI	45	BC77268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.48409
BC77269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84801	WGS84	BC77269	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77269_Cjap_Sim2018_COI	S8-19	1	BC77269_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77269	Sample	BC77269	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-19	COI	45	BC77269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49777
BC77269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84801	WGS84	BC77269	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77269_Mgig_Bot2012_COI	S8-19	1	BC77269_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77269	Sample	BC77269	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-19	COI	45	BC77269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.49777
BC77271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77271	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77271_Aamu_Bax2006_COI	S16-10	1	BC77271_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77271	Sample	BC77271	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-10	COI	45	BC77271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-10	GCACAACCGGGATCTTTACTTC	138.52039
BC77271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77271	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77271_Pcan_Bot2011_IGS	S16-10	1	BC77271_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77271	Sample	BC77271	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-10	IGS	45	BC77271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-10	CGTAATCCTCAGTACTGGCTA	138.52039
BC77271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77271	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77271_Sspa_Oph2007_COI	S16-10	1	BC77271_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77271	Sample	BC77271	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-10	COI	45	BC77271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-10	GAAGGCCTTCCGATGATC	138.52039
BC77271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77271	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77271_Upin_Bot2011_COI	S16-10	1	BC77271_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77271	Sample	BC77271	targeted	Absent	Sample	Upin_Bot2011_COI	S16-10	COI	45	BC77271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.52039
BC77272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8366	WGS84	BC77272	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77272_Cjap_Sim2018_COI	S16-02	1	BC77272_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77272	Sample	BC77272	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-02	COI	45	BC77272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50718
BC77272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8366	WGS84	BC77272	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.308	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77272_Mgig_Bot2012_COI	S16-02	1	BC77272_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77272	Sample	BC77272	targeted	Present	Sample	Mgig_Bot2012_COI	S16-02	COI	45	BC77272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.50718
BC77273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83638	WGS84	BC77273	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77273_Cmae_Bot2011_COI	S16-03	1	BC77273_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77273	Sample	BC77273	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-03	COI	45	BC77273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-03	ATGAACAGTCTATCCTCCTTTAG	138.50866
BC77275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83426	WGS84	BC77275	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77275_Aamu_Bax2006_COI	S16-05	1	BC77275_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77275	Sample	BC77275	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-05	COI	45	BC77275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-05	GCACAACCGGGATCTTTACTTC	138.50758
BC77275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83426	WGS84	BC77275	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77275_Pcan_Bot2011_IGS	S16-05	1	BC77275_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77275	Sample	BC77275	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-05	IGS	45	BC77275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-05	CGTAATCCTCAGTACTGGCTA	138.50758
BC77275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83426	WGS84	BC77275	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77275_Sspa_Oph2007_COI	S16-05	1	BC77275_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77275	Sample	BC77275	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-05	COI	45	BC77275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-05	GAAGGCCTTCCGATGATC	138.50758
BC77275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83426	WGS84	BC77275	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77275_Upin_Bot2011_COI	S16-05	1	BC77275_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77275	Sample	BC77275	targeted	Absent	Sample	Upin_Bot2011_COI	S16-05	COI	45	BC77275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.50758
BC77276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80831	WGS84	BC77276	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77276_Cjap_Sim2018_COI	S16-06	1	BC77276_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77276	Sample	BC77276	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-06	COI	45	BC77276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.516
BC77276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80831	WGS84	BC77276	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77276_Mgig_Bot2012_COI	S16-06	1	BC77276_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77276	Sample	BC77276	targeted	Absent	Sample	Mgig_Bot2012_COI	S16-06	COI	45	BC77276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.516
BC77277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77277	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77277_Cmae_Bot2011_COI	S16-07	1	BC77277_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77277	Sample	BC77277	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-07	COI	45	BC77277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-07	ATGAACAGTCTATCCTCCTTTAG	138.51754
BC77279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80992	WGS84	BC77279	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77279_Aamu_Bax2006_COI	S16-09	1	BC77279_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77279	Sample	BC77279	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-09	COI	45	BC77279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-09	GCACAACCGGGATCTTTACTTC	138.51928
BC77279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80992	WGS84	BC77279	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77279_Pcan_Bot2011_IGS	S16-09	1	BC77279_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77279	Sample	BC77279	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-09	IGS	45	BC77279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-09	CGTAATCCTCAGTACTGGCTA	138.51928
BC77279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80992	WGS84	BC77279	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77279_Sspa_Oph2007_COI	S16-09	1	BC77279_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77279	Sample	BC77279	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-09	COI	45	BC77279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-09	GAAGGCCTTCCGATGATC	138.51928
BC77279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80992	WGS84	BC77279	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77279_Upin_Bot2011_COI	S16-09	1	BC77279_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77279	Sample	BC77279	targeted	Absent	Sample	Upin_Bot2011_COI	S16-09	COI	45	BC77279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.51928
BC77280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83901	WGS84	BC77280	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77280_Cmae_Bot2011_COI	S16-01	1	BC77280_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77280	Sample	BC77280	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-01	COI	45	BC77280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-01	ATGAACAGTCTATCCTCCTTTAG	138.50604
BC77282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77369	WGS84	BC77282	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77282_Aamu_Bax2006_COI	S17-02	1	BC77282_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77282	Sample	BC77282	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-02	COI	45	BC77282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-02	GCACAACCGGGATCTTTACTTC	138.48594
BC77282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77369	WGS84	BC77282	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77282_Pcan_Bot2011_IGS	S17-02	1	BC77282_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77282	Sample	BC77282	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-02	IGS	45	BC77282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-02	CGTAATCCTCAGTACTGGCTA	138.48594
BC77282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77369	WGS84	BC77282	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.448	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77282_Sspa_Oph2007_COI	S17-02	1	BC77282_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77282	Sample	BC77282	targeted	Present	Sample	Sspa_Oph2007_COI	S17-02	COI	45	BC77282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-02	GAAGGCCTTCCGATGATC	138.48594
BC77282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77369	WGS84	BC77282	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77282_Upin_Bot2011_COI	S17-02	1	BC77282_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77282	Sample	BC77282	targeted	Absent	Sample	Upin_Bot2011_COI	S17-02	COI	45	BC77282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.48594
BC77283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77394	WGS84	BC77283	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77283_Cjap_Sim2018_COI	S17-03	1	BC77283_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77283	Sample	BC77283	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-03	COI	45	BC77283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48586
BC77283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77394	WGS84	BC77283	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.894	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77283_Mgig_Bot2012_COI	S17-03	1	BC77283_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77283	Sample	BC77283	targeted	Present	Sample	Mgig_Bot2012_COI	S17-03	COI	45	BC77283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.48586
BC77284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77691	WGS84	BC77284	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77284_Cmae_Bot2011_COI	S17-04	1	BC77284_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77284	Sample	BC77284	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-04	COI	45	BC77284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-04	ATGAACAGTCTATCCTCCTTTAG	138.51832
BC77286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77881	WGS84	BC77286	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77286_Aamu_Bax2006_COI	S17-06	1	BC77286_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77286	Sample	BC77286	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-06	COI	45	BC77286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-06	GCACAACCGGGATCTTTACTTC	138.51774
BC77286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77881	WGS84	BC77286	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77286_Pcan_Bot2011_IGS	S17-06	1	BC77286_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77286	Sample	BC77286	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-06	IGS	45	BC77286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-06	CGTAATCCTCAGTACTGGCTA	138.51774
BC77286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77881	WGS84	BC77286	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77286_Sspa_Oph2007_COI	S17-06	1	BC77286_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77286	Sample	BC77286	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-06	COI	45	BC77286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-06	GAAGGCCTTCCGATGATC	138.51774
BC77286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77881	WGS84	BC77286	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77286_Upin_Bot2011_COI	S17-06	1	BC77286_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77286	Sample	BC77286	targeted	Absent	Sample	Upin_Bot2011_COI	S17-06	COI	45	BC77286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.51774
BC77287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80962	WGS84	BC77287	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77287_Cjap_Sim2018_COI	S17-07	1	BC77287_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77287	Sample	BC77287	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-07	COI	45	BC77287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51817
BC77287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80962	WGS84	BC77287	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.583	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77287_Mgig_Bot2012_COI	S17-07	1	BC77287_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77287	Sample	BC77287	targeted	Present	Sample	Mgig_Bot2012_COI	S17-07	COI	45	BC77287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51817
BC77288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80998	WGS84	BC77288	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77288_Cmae_Bot2011_COI	S17-08	1	BC77288_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77288	Sample	BC77288	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-08	COI	45	BC77288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-08	ATGAACAGTCTATCCTCCTTTAG	138.51955
BC77290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84498	WGS84	BC77290	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77290_Cjap_Sim2018_COI	S17-10	1	BC77290_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77290	Sample	BC77290	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-10	COI	45	BC77290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49722
BC77290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84498	WGS84	BC77290	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77290_Mgig_Bot2012_COI	S17-10	1	BC77290_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77290	Sample	BC77290	targeted	Absent	Sample	Mgig_Bot2012_COI	S17-10	COI	45	BC77290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.49722
BC77291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.844	WGS84	BC77291	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77291_Cmae_Bot2011_COI	S17-11	1	BC77291_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77291	Sample	BC77291	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-11	COI	45	BC77291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-11	ATGAACAGTCTATCCTCCTTTAG	138.4977
BC77293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8367	WGS84	BC77293	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77293_Aamu_Bax2006_COI	S17-13	1	BC77293_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77293	Sample	BC77293	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-13	COI	45	BC77293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-13	GCACAACCGGGATCTTTACTTC	138.50883
BC77293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8367	WGS84	BC77293	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77293_Pcan_Bot2011_IGS	S17-13	1	BC77293_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77293	Sample	BC77293	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-13	IGS	45	BC77293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-13	CGTAATCCTCAGTACTGGCTA	138.50883
BC77293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8367	WGS84	BC77293	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77293_Sspa_Oph2007_COI	S17-13	1	BC77293_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77293	Sample	BC77293	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-13	COI	45	BC77293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-13	GAAGGCCTTCCGATGATC	138.50883
BC77293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8367	WGS84	BC77293	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77293_Upin_Bot2011_COI	S17-13	1	BC77293_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77293	Sample	BC77293	targeted	Absent	Sample	Upin_Bot2011_COI	S17-13	COI	45	BC77293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.50883
BC77294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83721	WGS84	BC77294	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77294_Cjap_Sim2018_COI	S17-14	1	BC77294_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77294	Sample	BC77294	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-14	COI	45	BC77294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51088
BC77294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83721	WGS84	BC77294	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.38	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77294_Mgig_Bot2012_COI	S17-14	1	BC77294_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77294	Sample	BC77294	targeted	Present	Sample	Mgig_Bot2012_COI	S17-14	COI	45	BC77294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.51088
BC77295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83581	WGS84	BC77295	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77295_Cmae_Bot2011_COI	S17-15	1	BC77295_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77295	Sample	BC77295	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-15	COI	45	BC77295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-15	ATGAACAGTCTATCCTCCTTTAG	138.50807
BC77297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83622	WGS84	BC77297	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77297_Aamu_Bax2006_COI	S18-02	1	BC77297_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77297	Sample	BC77297	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-02	COI	45	BC77297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-02	GCACAACCGGGATCTTTACTTC	138.50888
BC77297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83622	WGS84	BC77297	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77297_Pcan_Bot2011_IGS	S18-02	1	BC77297_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77297	Sample	BC77297	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-02	IGS	45	BC77297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-02	CGTAATCCTCAGTACTGGCTA	138.50888
BC77297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83622	WGS84	BC77297	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.76	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77297_Sspa_Oph2007_COI	S18-02	1	BC77297_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77297	Sample	BC77297	targeted	Present	Sample	Sspa_Oph2007_COI	S18-02	COI	45	BC77297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-02	GAAGGCCTTCCGATGATC	138.50888
BC77297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83622	WGS84	BC77297	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77297_Upin_Bot2011_COI	S18-02	1	BC77297_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77297	Sample	BC77297	targeted	Absent	Sample	Upin_Bot2011_COI	S18-02	COI	45	BC77297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.50888
BC77298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83661	WGS84	BC77298	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77298_Cjap_Sim2018_COI	S18-03	1	BC77298_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77298	Sample	BC77298	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-03	COI	45	BC77298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5099
BC77298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83661	WGS84	BC77298	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.339	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77298_Mgig_Bot2012_COI	S18-03	1	BC77298_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77298	Sample	BC77298	targeted	Present	Sample	Mgig_Bot2012_COI	S18-03	COI	45	BC77298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.5099
BC77299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83696	WGS84	BC77299	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77299_Cmae_Bot2011_COI	S18-04	1	BC77299_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77299	Sample	BC77299	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-04	COI	45	BC77299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-04	ATGAACAGTCTATCCTCCTTTAG	138.51075
BC77301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80836	WGS84	BC77301	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77301_Aamu_Bax2006_COI	S18-06	1	BC77301_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77301	Sample	BC77301	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-06	COI	45	BC77301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-06	GCACAACCGGGATCTTTACTTC	138.51628
BC77301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80836	WGS84	BC77301	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77301_Pcan_Bot2011_IGS	S18-06	1	BC77301_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77301	Sample	BC77301	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-06	IGS	45	BC77301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-06	CGTAATCCTCAGTACTGGCTA	138.51628
BC77301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80836	WGS84	BC77301	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.38	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77301_Sspa_Oph2007_COI	S18-06	1	BC77301_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77301	Sample	BC77301	targeted	Present	Sample	Sspa_Oph2007_COI	S18-06	COI	45	BC77301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-06	GAAGGCCTTCCGATGATC	138.51628
BC77301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80836	WGS84	BC77301	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77301_Upin_Bot2011_COI	S18-06	1	BC77301_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77301	Sample	BC77301	targeted	Absent	Sample	Upin_Bot2011_COI	S18-06	COI	45	BC77301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.51628
BC77302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77302	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77302_Cjap_Sim2018_COI	S18-07	1	BC77302_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77302	Sample	BC77302	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-07	COI	45	BC77302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51771
BC77302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77302	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77302_Mgig_Bot2012_COI	S18-07	1	BC77302_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77302	Sample	BC77302	targeted	Absent	Sample	Mgig_Bot2012_COI	S18-07	COI	45	BC77302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51771
BC77303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77303	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77303_Cmae_Bot2011_COI	S18-08	1	BC77303_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77303	Sample	BC77303	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-08	COI	45	BC77303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-08	ATGAACAGTCTATCCTCCTTTAG	138.51854
BC77305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77305	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77305_Aamu_Bax2006_COI	S18-10	1	BC77305_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77305	Sample	BC77305	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-10	COI	45	BC77305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-10	GCACAACCGGGATCTTTACTTC	138.5198
BC77305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77305	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77305_Pcan_Bot2011_IGS	S18-10	1	BC77305_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77305	Sample	BC77305	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-10	IGS	45	BC77305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-10	CGTAATCCTCAGTACTGGCTA	138.5198
BC77305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77305	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.337	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77305_Sspa_Oph2007_COI	S18-10	1	BC77305_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77305	Sample	BC77305	targeted	Present	Sample	Sspa_Oph2007_COI	S18-10	COI	45	BC77305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-10	GAAGGCCTTCCGATGATC	138.5198
BC77305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77305	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77305_Upin_Bot2011_COI	S18-10	1	BC77305_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77305	Sample	BC77305	targeted	Absent	Sample	Upin_Bot2011_COI	S18-10	COI	45	BC77305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.5198
BC77306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77317	WGS84	BC77306	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77306_Cjap_Sim2018_COI	S19-01	1	BC77306_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77306	Sample	BC77306	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-01	COI	45	BC77306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48557
BC77306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77317	WGS84	BC77306	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77306_Mgig_Bot2012_COI	S19-01	1	BC77306_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77306	Sample	BC77306	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-01	COI	45	BC77306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.48557
BC77307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77357	WGS84	BC77307	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.727	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77307_Cmae_Bot2011_COI	S19-02	1	BC77307_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77307	Sample	BC77307	targeted	Present	Sample	Cmae_Bot2011_COI	S19-02	COI	45	BC77307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-02	ATGAACAGTCTATCCTCCTTTAG	138.48591
BC77309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77781	WGS84	BC77309	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77309_Aamu_Bax2006_COI	S19-04	1	BC77309_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77309	Sample	BC77309	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-04	COI	45	BC77309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-04	GCACAACCGGGATCTTTACTTC	138.51905
BC77309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77781	WGS84	BC77309	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77309_Pcan_Bot2011_IGS	S19-04	1	BC77309_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77309	Sample	BC77309	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-04	IGS	45	BC77309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-04	CGTAATCCTCAGTACTGGCTA	138.51905
BC77309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77781	WGS84	BC77309	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.929	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77309_Sspa_Oph2007_COI	S19-04	1	BC77309_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77309	Sample	BC77309	targeted	Present	Sample	Sspa_Oph2007_COI	S19-04	COI	45	BC77309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-04	GAAGGCCTTCCGATGATC	138.51905
BC77309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77781	WGS84	BC77309	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77309_Upin_Bot2011_COI	S19-04	1	BC77309_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77309	Sample	BC77309	targeted	Absent	Sample	Upin_Bot2011_COI	S19-04	COI	45	BC77309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.51905
BC77310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7798	WGS84	BC77310	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77310_Cmae_Bot2011_COI	S19-05	1	BC77310_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77310	Sample	BC77310	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-05	COI	45	BC77310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-05	ATGAACAGTCTATCCTCCTTTAG	138.51801
BC77312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84678	WGS84	BC77312	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77312_Aamu_Bax2006_COI	S19-07	1	BC77312_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77312	Sample	BC77312	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-07	COI	45	BC77312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-07	GCACAACCGGGATCTTTACTTC	138.49755
BC77312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84678	WGS84	BC77312	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77312_Pcan_Bot2011_IGS	S19-07	1	BC77312_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77312	Sample	BC77312	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-07	IGS	45	BC77312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-07	CGTAATCCTCAGTACTGGCTA	138.49755
BC77312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84678	WGS84	BC77312	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.035	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77312_Sspa_Oph2007_COI	S19-07	1	BC77312_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77312	Sample	BC77312	targeted	Present	Sample	Sspa_Oph2007_COI	S19-07	COI	45	BC77312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-07	GAAGGCCTTCCGATGATC	138.49755
BC77312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84678	WGS84	BC77312	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77312_Upin_Bot2011_COI	S19-07	1	BC77312_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77312	Sample	BC77312	targeted	Absent	Sample	Upin_Bot2011_COI	S19-07	COI	45	BC77312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.49755
BC77313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84552	WGS84	BC77313	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77313_Cjap_Sim2018_COI	S19-08	1	BC77313_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77313	Sample	BC77313	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-08	COI	45	BC77313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49703
BC77313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84552	WGS84	BC77313	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77313_Mgig_Bot2012_COI	S19-08	1	BC77313_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77313	Sample	BC77313	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-08	COI	45	BC77313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.49703
BC77314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84334	WGS84	BC77314	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77314_Cmae_Bot2011_COI	S19-09	1	BC77314_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77314	Sample	BC77314	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-09	COI	45	BC77314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-09	ATGAACAGTCTATCCTCCTTTAG	138.49677
BC77316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83711	WGS84	BC77316	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77316_Aamu_Bax2006_COI	S19-11	1	BC77316_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77316	Sample	BC77316	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-11	COI	45	BC77316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-11	GCACAACCGGGATCTTTACTTC	138.50972
BC77316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83711	WGS84	BC77316	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77316_Pcan_Bot2011_IGS	S19-11	1	BC77316_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77316	Sample	BC77316	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-11	IGS	45	BC77316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-11	CGTAATCCTCAGTACTGGCTA	138.50972
BC77316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83711	WGS84	BC77316	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.822	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77316_Sspa_Oph2007_COI	S19-11	1	BC77316_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77316	Sample	BC77316	targeted	Present	Sample	Sspa_Oph2007_COI	S19-11	COI	45	BC77316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-11	GAAGGCCTTCCGATGATC	138.50972
BC77316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83711	WGS84	BC77316	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77316_Upin_Bot2011_COI	S19-11	1	BC77316_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77316	Sample	BC77316	targeted	Absent	Sample	Upin_Bot2011_COI	S19-11	COI	45	BC77316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.50972
BC77317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83647	WGS84	BC77317	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77317_Cjap_Sim2018_COI	S19-12	1	BC77317_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77317	Sample	BC77317	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-12	COI	45	BC77317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50948
BC77317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83647	WGS84	BC77317	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77317_Mgig_Bot2012_COI	S19-12	1	BC77317_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77317	Sample	BC77317	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-12	COI	45	BC77317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.50948
BC77318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8092	WGS84	BC77318	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77318_Cmae_Bot2011_COI	S19-13	1	BC77318_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77318	Sample	BC77318	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-13	COI	45	BC77318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-13	ATGAACAGTCTATCCTCCTTTAG	138.5177
BC77320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81046	WGS84	BC77320	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77320_Cjap_Sim2018_COI	S19-15	1	BC77320_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77320	Sample	BC77320	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-15	COI	45	BC77320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52139
BC77320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81046	WGS84	BC77320	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77320_Mgig_Bot2012_COI	S19-15	1	BC77320_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77320	Sample	BC77320	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-15	COI	45	BC77320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.52139
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28890_Aamu_Bax2006_COI	POR-B29	1	BD28890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	GCACAACCGGGATCTTTACTTC	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28890_Pcan_Bot2011_IGS	POR-B29	1	BD28890_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B29	IGS	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	CGTAATCCTCAGTACTGGCTA	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28890_Pper_Dia2013_COI	POR-B29	1	BD28890_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	CTTAGTGGCATTAATTCGDAATCC	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28890_Sspa_Oph2007_COI	POR-B29	1	BD28890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	GAAGGCCTTCCGATGATC	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.781	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28890_Upin_Bot2011_COI	POR-B29	1	BD28890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Present	Sample	Upin_Bot2011_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	CTTTAATTACAGCGTTTTTATTGTTGT	141.62107
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28891_Cjap_Sim2018_COI	POR-B30	1	BD28891_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28891_Esin_And2018_Cyb	POR-B30	1	BD28891_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B30	Cytb	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	ACCCCTCCTCATATCCAACCA	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28891_Mgig_Bot2012_COI	POR-B30	1	BD28891_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	TCTTATTCGTTGGAGACTTTATAACCCT	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28891_Mjap_Gib2022_COI	POR-B30	1	BD28891_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	AATTTAATTGTGACTAGGCATGGTCTG	141.62277
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28892_Cmae_Bot2011_COI	POR-B31	1	BD28892_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	ATGAACAGTCTATCCTCCTTTAG	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28892_Hsan_Wil2021_COI	POR-B31	1	BD28892_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	CCTGGGCCGGTATAGTAGGT	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28892_Mros_Gib2022_COI	POR-B31	1	BD28892_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28892_Msal_Bot2012_COI	POR-B31	1	BD28892_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	GYTAGTTCCRATGATGTTAGCTG	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28892_Mstr_Wil2021_COI	POR-B31	1	BD28892_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	GTAGTGGTAACTACTCATGCTTTA	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28892_Pvir_Dia2013_COI	POR-B31	1	BD28892_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	CTTAGTGGCATTAATTCGDAATCC	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28892_Rhar_Sim2018_COI	POR-B31	1	BD28892_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	CTGCTGCTATTGCTCATG	141.61982
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28894_Aamu_Bax2006_COI	POR-B33	1	BD28894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	GCACAACCGGGATCTTTACTTC	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28894_Pcan_Bot2011_IGS	POR-B33	1	BD28894_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B33	IGS	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	CGTAATCCTCAGTACTGGCTA	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28894_Pper_Dia2013_COI	POR-B33	1	BD28894_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	CTTAGTGGCATTAATTCGDAATCC	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28894_Sspa_Oph2007_COI	POR-B33	1	BD28894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	GAAGGCCTTCCGATGATC	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.232	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28894_Upin_Bot2011_COI	POR-B33	1	BD28894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Present	Sample	Upin_Bot2011_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	CTTTAATTACAGCGTTTTTATTGTTGT	141.61708
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28895_Cjap_Sim2018_COI	POR-B34	1	BD28895_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28895_Esin_And2018_Cyb	POR-B34	1	BD28895_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B34	Cytb	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	ACCCCTCCTCATATCCAACCA	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28895_Mgig_Bot2012_COI	POR-B34	1	BD28895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	TCTTATTCGTTGGAGACTTTATAACCCT	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28895_Mjap_Gib2022_COI	POR-B34	1	BD28895_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	AATTTAATTGTGACTAGGCATGGTCTG	141.61843
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28896_Cmae_Bot2011_COI	POR-B22	1	BD28896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	ATGAACAGTCTATCCTCCTTTAG	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28896_Hsan_Wil2021_COI	POR-B22	1	BD28896_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	CCTGGGCCGGTATAGTAGGT	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28896_Mros_Gib2022_COI	POR-B22	1	BD28896_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28896_Msal_Bot2012_COI	POR-B22	1	BD28896_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	GYTAGTTCCRATGATGTTAGCTG	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28896_Mstr_Wil2021_COI	POR-B22	1	BD28896_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	GTAGTGGTAACTACTCATGCTTTA	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28896_Pvir_Dia2013_COI	POR-B22	1	BD28896_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	CTTAGTGGCATTAATTCGDAATCC	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28896_Rhar_Sim2018_COI	POR-B22	1	BD28896_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	CTGCTGCTATTGCTCATG	141.62235
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28898_Aamu_Bax2006_COI	POR-B20	1	BD28898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	GCACAACCGGGATCTTTACTTC	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28898_Pcan_Bot2011_IGS	POR-B20	1	BD28898_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B20	IGS	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	CGTAATCCTCAGTACTGGCTA	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28898_Pper_Dia2013_COI	POR-B20	1	BD28898_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	CTTAGTGGCATTAATTCGDAATCC	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28898_Sspa_Oph2007_COI	POR-B20	1	BD28898_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	GAAGGCCTTCCGATGATC	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.388	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28898_Upin_Bot2011_COI	POR-B20	1	BD28898_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Present	Sample	Upin_Bot2011_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	CTTTAATTACAGCGTTTTTATTGTTGT	141.62028
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28899_Cjap_Sim2018_COI	POR-B19	1	BD28899_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28899_Esin_And2018_Cyb	POR-B19	1	BD28899_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B19	Cytb	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	ACCCCTCCTCATATCCAACCA	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28899_Mgig_Bot2012_COI	POR-B19	1	BD28899_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	TCTTATTCGTTGGAGACTTTATAACCCT	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28899_Mjap_Gib2022_COI	POR-B19	1	BD28899_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	AATTTAATTGTGACTAGGCATGGTCTG	141.6189
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28900_Cmae_Bot2011_COI	POR-B18	1	BD28900_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	ATGAACAGTCTATCCTCCTTTAG	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28900_Hsan_Wil2021_COI	POR-B18	1	BD28900_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	CCTGGGCCGGTATAGTAGGT	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28900_Mros_Gib2022_COI	POR-B18	1	BD28900_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28900_Msal_Bot2012_COI	POR-B18	1	BD28900_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	GYTAGTTCCRATGATGTTAGCTG	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28900_Mstr_Wil2021_COI	POR-B18	1	BD28900_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	GTAGTGGTAACTACTCATGCTTTA	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28900_Pvir_Dia2013_COI	POR-B18	1	BD28900_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	CTTAGTGGCATTAATTCGDAATCC	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28900_Rhar_Sim2018_COI	POR-B18	1	BD28900_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	CTGCTGCTATTGCTCATG	141.61759
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28902_Aamu_Bax2006_COI	POR-B16	1	BD28902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	GCACAACCGGGATCTTTACTTC	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28902_Pcan_Bot2011_IGS	POR-B16	1	BD28902_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B16	IGS	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	CGTAATCCTCAGTACTGGCTA	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28902_Pper_Dia2013_COI	POR-B16	1	BD28902_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	CTTAGTGGCATTAATTCGDAATCC	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28902_Sspa_Oph2007_COI	POR-B16	1	BD28902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	GAAGGCCTTCCGATGATC	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.485	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28902_Upin_Bot2011_COI	POR-B16	1	BD28902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Present	Sample	Upin_Bot2011_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	CTTTAATTACAGCGTTTTTATTGTTGT	141.6148
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28903_Cjap_Sim2018_COI	POR-B15	1	BD28903_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28903_Esin_And2018_Cyb	POR-B15	1	BD28903_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B15	Cytb	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	ACCCCTCCTCATATCCAACCA	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28903_Mgig_Bot2012_COI	POR-B15	1	BD28903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	TCTTATTCGTTGGAGACTTTATAACCCT	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28903_Mjap_Gib2022_COI	POR-B15	1	BD28903_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	AATTTAATTGTGACTAGGCATGGTCTG	141.61341
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28904_Cmae_Bot2011_COI	POR-B14	1	BD28904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	ATGAACAGTCTATCCTCCTTTAG	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28904_Hsan_Wil2021_COI	POR-B14	1	BD28904_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	CCTGGGCCGGTATAGTAGGT	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28904_Mros_Gib2022_COI	POR-B14	1	BD28904_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28904_Msal_Bot2012_COI	POR-B14	1	BD28904_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	GYTAGTTCCRATGATGTTAGCTG	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28904_Mstr_Wil2021_COI	POR-B14	1	BD28904_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	GTAGTGGTAACTACTCATGCTTTA	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28904_Pvir_Dia2013_COI	POR-B14	1	BD28904_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	CTTAGTGGCATTAATTCGDAATCC	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28904_Rhar_Sim2018_COI	POR-B14	1	BD28904_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	CTGCTGCTATTGCTCATG	141.61401
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28906_Aamu_Bax2006_COI	POR-B12	1	BD28906_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	GCACAACCGGGATCTTTACTTC	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28906_Pcan_Bot2011_IGS	POR-B12	1	BD28906_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B12	IGS	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	CGTAATCCTCAGTACTGGCTA	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28906_Pper_Dia2013_COI	POR-B12	1	BD28906_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	CTTAGTGGCATTAATTCGDAATCC	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28906_Sspa_Oph2007_COI	POR-B12	1	BD28906_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	GAAGGCCTTCCGATGATC	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.659	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28906_Upin_Bot2011_COI	POR-B12	1	BD28906_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Present	Sample	Upin_Bot2011_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	CTTTAATTACAGCGTTTTTATTGTTGT	141.61129
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28907_Cjap_Sim2018_COI	POR-B11	1	BD28907_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28907_Esin_And2018_Cyb	POR-B11	1	BD28907_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B11	Cytb	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	ACCCCTCCTCATATCCAACCA	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28907_Mgig_Bot2012_COI	POR-B11	1	BD28907_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	TCTTATTCGTTGGAGACTTTATAACCCT	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28907_Mjap_Gib2022_COI	POR-B11	1	BD28907_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	AATTTAATTGTGACTAGGCATGGTCTG	141.62078
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28910_Cjap_Sim2018_COI	POR-B09	1	BD28910_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28910_Esin_And2018_Cyb	POR-B09	1	BD28910_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B09	Cytb	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	ACCCCTCCTCATATCCAACCA	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28910_Mgig_Bot2012_COI	POR-B09	1	BD28910_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	TCTTATTCGTTGGAGACTTTATAACCCT	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28910_Mjap_Gib2022_COI	POR-B09	1	BD28910_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	AATTTAATTGTGACTAGGCATGGTCTG	141.61892
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28911_Cmae_Bot2011_COI	POR-B08	1	BD28911_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	ATGAACAGTCTATCCTCCTTTAG	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28911_Hsan_Wil2021_COI	POR-B08	1	BD28911_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	CCTGGGCCGGTATAGTAGGT	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28911_Mros_Gib2022_COI	POR-B08	1	BD28911_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28911_Msal_Bot2012_COI	POR-B08	1	BD28911_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	GYTAGTTCCRATGATGTTAGCTG	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28911_Mstr_Wil2021_COI	POR-B08	1	BD28911_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	GTAGTGGTAACTACTCATGCTTTA	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28911_Pvir_Dia2013_COI	POR-B08	1	BD28911_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	CTTAGTGGCATTAATTCGDAATCC	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28911_Rhar_Sim2018_COI	POR-B08	1	BD28911_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	CTGCTGCTATTGCTCATG	141.61753
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28913_Aamu_Bax2006_COI	POR-B06	1	BD28913_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	GCACAACCGGGATCTTTACTTC	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28913_Pcan_Bot2011_IGS	POR-B06	1	BD28913_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B06	IGS	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	CGTAATCCTCAGTACTGGCTA	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28913_Pper_Dia2013_COI	POR-B06	1	BD28913_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	CTTAGTGGCATTAATTCGDAATCC	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28913_Sspa_Oph2007_COI	POR-B06	1	BD28913_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	GAAGGCCTTCCGATGATC	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.226	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28913_Upin_Bot2011_COI	POR-B06	1	BD28913_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Present	Sample	Upin_Bot2011_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	CTTTAATTACAGCGTTTTTATTGTTGT	141.61473
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28914_Cjap_Sim2018_COI	POR-B05	1	BD28914_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28914_Esin_And2018_Cyb	POR-B05	1	BD28914_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B05	Cytb	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	ACCCCTCCTCATATCCAACCA	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28914_Mgig_Bot2012_COI	POR-B05	1	BD28914_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	TCTTATTCGTTGGAGACTTTATAACCCT	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28914_Mjap_Gib2022_COI	POR-B05	1	BD28914_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	AATTTAATTGTGACTAGGCATGGTCTG	141.61197
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28915_Cmae_Bot2011_COI	POR-B04	1	BD28915_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	ATGAACAGTCTATCCTCCTTTAG	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28915_Hsan_Wil2021_COI	POR-B04	1	BD28915_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	CCTGGGCCGGTATAGTAGGT	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.96	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28915_Mros_Gib2022_COI	POR-B04	1	BD28915_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Present	Sample	Mros_Gib2022_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28915_Msal_Bot2012_COI	POR-B04	1	BD28915_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	GYTAGTTCCRATGATGTTAGCTG	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28915_Mstr_Wil2021_COI	POR-B04	1	BD28915_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	GTAGTGGTAACTACTCATGCTTTA	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28915_Pvir_Dia2013_COI	POR-B04	1	BD28915_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	CTTAGTGGCATTAATTCGDAATCC	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28915_Rhar_Sim2018_COI	POR-B04	1	BD28915_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	CTGCTGCTATTGCTCATG	141.61159
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28917_Aamu_Bax2006_COI	POR-B02	1	BD28917_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	GCACAACCGGGATCTTTACTTC	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28917_Pcan_Bot2011_IGS	POR-B02	1	BD28917_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B02	IGS	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	CGTAATCCTCAGTACTGGCTA	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28917_Pper_Dia2013_COI	POR-B02	1	BD28917_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	CTTAGTGGCATTAATTCGDAATCC	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.476	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28917_Sspa_Oph2007_COI	POR-B02	1	BD28917_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Present	Sample	Sspa_Oph2007_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	GAAGGCCTTCCGATGATC	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.167	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28917_Upin_Bot2011_COI	POR-B02	1	BD28917_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Present	Sample	Upin_Bot2011_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	CTTTAATTACAGCGTTTTTATTGTTGT	141.61065
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28918_Cjap_Sim2018_COI	POR-B01	1	BD28918_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28918_Esin_And2018_Cyb	POR-B01	1	BD28918_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B01	Cytb	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	ACCCCTCCTCATATCCAACCA	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28918_Mgig_Bot2012_COI	POR-B01	1	BD28918_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	TCTTATTCGTTGGAGACTTTATAACCCT	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28918_Mjap_Gib2022_COI	POR-B01	1	BD28918_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	AATTTAATTGTGACTAGGCATGGTCTG	141.60984
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28919_Cmae_Bot2011_COI	ADL-B35	1	BD28919_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	ATGAACAGTCTATCCTCCTTTAG	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28919_Hsan_Wil2021_COI	ADL-B35	1	BD28919_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	CCTGGGCCGGTATAGTAGGT	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28919_Mros_Gib2022_COI	ADL-B35	1	BD28919_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28919_Msal_Bot2012_COI	ADL-B35	1	BD28919_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	GYTAGTTCCRATGATGTTAGCTG	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28919_Mstr_Wil2021_COI	ADL-B35	1	BD28919_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	GTAGTGGTAACTACTCATGCTTTA	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28919_Pvir_Dia2013_COI	ADL-B35	1	BD28919_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	CTTAGTGGCATTAATTCGDAATCC	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28919_Rhar_Sim2018_COI	ADL-B35	1	BD28919_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	CTGCTGCTATTGCTCATG	138.51463
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28920_Aamu_Bax2006_COI	ADL-B34	1	BD28920_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	GCACAACCGGGATCTTTACTTC	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28920_Pcan_Bot2011_IGS	ADL-B34	1	BD28920_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B34	IGS	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	CGTAATCCTCAGTACTGGCTA	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28920_Pper_Dia2013_COI	ADL-B34	1	BD28920_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	CTTAGTGGCATTAATTCGDAATCC	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28920_Sspa_Oph2007_COI	ADL-B34	1	BD28920_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	GAAGGCCTTCCGATGATC	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28920_Upin_Bot2011_COI	ADL-B34	1	BD28920_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	CTTTAATTACAGCGTTTTTATTGTTGT	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81218	WGS84	BD28920	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28920_Vgib_Wil2025_COI	ADL-B34	1	BD28920_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	CCCCTCTTTACAAGTTCTTTAGGGT	138.514
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81419	WGS84	BD28921	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28921_Asen_Wil2025_COI	ADL-B33	1	BD28921_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	GTTGTTGTTACAACACATGCTCTTTTG	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28921_Cjap_Sim2018_COI	ADL-B33	1	BD28921_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28921_Esin_And2018_Cyb	ADL-B33	1	BD28921_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B33	Cytb	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	ACCCCTCCTCATATCCAACCA	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.537	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28921_Mgig_Bot2012_COI	ADL-B33	1	BD28921_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	TCTTATTCGTTGGAGACTTTATAACCCT	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28921_Mjap_Gib2022_COI	ADL-B33	1	BD28921_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	AATTTAATTGTGACTAGGCATGGTCTG	138.51399
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28922_Cmae_Bot2011_COI	ADL-B32	1	BD28922_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	ATGAACAGTCTATCCTCCTTTAG	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28922_Hsan_Wil2021_COI	ADL-B32	1	BD28922_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	CCTGGGCCGGTATAGTAGGT	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28922_Mros_Gib2022_COI	ADL-B32	1	BD28922_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28922_Msal_Bot2012_COI	ADL-B32	1	BD28922_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	GYTAGTTCCRATGATGTTAGCTG	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28922_Mstr_Wil2021_COI	ADL-B32	1	BD28922_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	GTAGTGGTAACTACTCATGCTTTA	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28922_Pvir_Dia2013_COI	ADL-B32	1	BD28922_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	CTTAGTGGCATTAATTCGDAATCC	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28922_Rhar_Sim2018_COI	ADL-B32	1	BD28922_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	CTGCTGCTATTGCTCATG	138.51323
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28924_Aamu_Bax2006_COI	ADL-B30	1	BD28924_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	GCACAACCGGGATCTTTACTTC	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28924_Pcan_Bot2011_IGS	ADL-B30	1	BD28924_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B30	IGS	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	CGTAATCCTCAGTACTGGCTA	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28924_Pper_Dia2013_COI	ADL-B30	1	BD28924_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	CTTAGTGGCATTAATTCGDAATCC	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28924_Sspa_Oph2007_COI	ADL-B30	1	BD28924_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	GAAGGCCTTCCGATGATC	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28924_Upin_Bot2011_COI	ADL-B30	1	BD28924_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	CTTTAATTACAGCGTTTTTATTGTTGT	138.51328
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28925_Cjap_Sim2018_COI	ADL-B29	1	BD28925_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28925_Esin_And2018_Cyb	ADL-B29	1	BD28925_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B29	Cytb	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	ACCCCTCCTCATATCCAACCA	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.275	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28925_Mgig_Bot2012_COI	ADL-B29	1	BD28925_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	TCTTATTCGTTGGAGACTTTATAACCCT	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28925_Mjap_Gib2022_COI	ADL-B29	1	BD28925_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	AATTTAATTGTGACTAGGCATGGTCTG	138.51263
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28926_Cmae_Bot2011_COI	ADL-B28	1	BD28926_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	ATGAACAGTCTATCCTCCTTTAG	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28926_Hsan_Wil2021_COI	ADL-B28	1	BD28926_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	CCTGGGCCGGTATAGTAGGT	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28926_Mros_Gib2022_COI	ADL-B28	1	BD28926_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28926_Msal_Bot2012_COI	ADL-B28	1	BD28926_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	GYTAGTTCCRATGATGTTAGCTG	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28926_Mstr_Wil2021_COI	ADL-B28	1	BD28926_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	GTAGTGGTAACTACTCATGCTTTA	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28926_Pvir_Dia2013_COI	ADL-B28	1	BD28926_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	CTTAGTGGCATTAATTCGDAATCC	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28926_Rhar_Sim2018_COI	ADL-B28	1	BD28926_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	CTGCTGCTATTGCTCATG	138.51205
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28928_Aamu_Bax2006_COI	ADL-B26	1	BD28928_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	GCACAACCGGGATCTTTACTTC	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28928_Pcan_Bot2011_IGS	ADL-B26	1	BD28928_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B26	IGS	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	CGTAATCCTCAGTACTGGCTA	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28928_Pper_Dia2013_COI	ADL-B26	1	BD28928_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	CTTAGTGGCATTAATTCGDAATCC	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28928_Sspa_Oph2007_COI	ADL-B26	1	BD28928_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	GAAGGCCTTCCGATGATC	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28928_Upin_Bot2011_COI	ADL-B26	1	BD28928_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	CTTTAATTACAGCGTTTTTATTGTTGT	138.51074
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28929_Cjap_Sim2018_COI	ADL-B25	1	BD28929_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28929_Esin_And2018_Cyb	ADL-B25	1	BD28929_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B25	Cytb	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	ACCCCTCCTCATATCCAACCA	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.123	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28929_Mgig_Bot2012_COI	ADL-B25	1	BD28929_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	TCTTATTCGTTGGAGACTTTATAACCCT	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28929_Mjap_Gib2022_COI	ADL-B25	1	BD28929_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	AATTTAATTGTGACTAGGCATGGTCTG	138.51172
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28931_Aamu_Bax2006_COI	ADL-B23	1	BD28931_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	GCACAACCGGGATCTTTACTTC	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28931_Pcan_Bot2011_IGS	ADL-B23	1	BD28931_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B23	IGS	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	CGTAATCCTCAGTACTGGCTA	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28931_Pper_Dia2013_COI	ADL-B23	1	BD28931_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	CTTAGTGGCATTAATTCGDAATCC	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28931_Sspa_Oph2007_COI	ADL-B23	1	BD28931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	GAAGGCCTTCCGATGATC	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28931_Upin_Bot2011_COI	ADL-B23	1	BD28931_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	CTTTAATTACAGCGTTTTTATTGTTGT	138.51105
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28932_Cjap_Sim2018_COI	ADL-B22	1	BD28932_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28932_Esin_And2018_Cyb	ADL-B22	1	BD28932_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B22	Cytb	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	ACCCCTCCTCATATCCAACCA	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.191	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28932_Mgig_Bot2012_COI	ADL-B22	1	BD28932_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	TCTTATTCGTTGGAGACTTTATAACCCT	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28932_Mjap_Gib2022_COI	ADL-B22	1	BD28932_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	AATTTAATTGTGACTAGGCATGGTCTG	138.50953
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28933_Cmae_Bot2011_COI	ADL-B21	1	BD28933_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	ATGAACAGTCTATCCTCCTTTAG	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28933_Hsan_Wil2021_COI	ADL-B21	1	BD28933_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	CCTGGGCCGGTATAGTAGGT	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28933_Mros_Gib2022_COI	ADL-B21	1	BD28933_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28933_Msal_Bot2012_COI	ADL-B21	1	BD28933_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	GYTAGTTCCRATGATGTTAGCTG	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28933_Mstr_Wil2021_COI	ADL-B21	1	BD28933_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	GTAGTGGTAACTACTCATGCTTTA	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28933_Pvir_Dia2013_COI	ADL-B21	1	BD28933_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	CTTAGTGGCATTAATTCGDAATCC	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28933_Rhar_Sim2018_COI	ADL-B21	1	BD28933_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	CTGCTGCTATTGCTCATG	138.51072
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28935_Aamu_Bax2006_COI	ADL-B19	1	BD28935_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	GCACAACCGGGATCTTTACTTC	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28935_Pcan_Bot2011_IGS	ADL-B19	1	BD28935_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B19	IGS	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	CGTAATCCTCAGTACTGGCTA	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28935_Pper_Dia2013_COI	ADL-B19	1	BD28935_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	CTTAGTGGCATTAATTCGDAATCC	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.972	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28935_Sspa_Oph2007_COI	ADL-B19	1	BD28935_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	GAAGGCCTTCCGATGATC	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28935_Upin_Bot2011_COI	ADL-B19	1	BD28935_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	CTTTAATTACAGCGTTTTTATTGTTGT	138.50973
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28936_Cjap_Sim2018_COI	ADL-B18	1	BD28936_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28936_Esin_And2018_Cyb	ADL-B18	1	BD28936_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B18	Cytb	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	ACCCCTCCTCATATCCAACCA	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.137	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28936_Mgig_Bot2012_COI	ADL-B18	1	BD28936_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	TCTTATTCGTTGGAGACTTTATAACCCT	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28936_Mjap_Gib2022_COI	ADL-B18	1	BD28936_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	AATTTAATTGTGACTAGGCATGGTCTG	138.50817
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28937_Cmae_Bot2011_COI	ADL-B17	1	BD28937_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	ATGAACAGTCTATCCTCCTTTAG	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28937_Hsan_Wil2021_COI	ADL-B17	1	BD28937_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	CCTGGGCCGGTATAGTAGGT	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28937_Mros_Gib2022_COI	ADL-B17	1	BD28937_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28937_Msal_Bot2012_COI	ADL-B17	1	BD28937_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	GYTAGTTCCRATGATGTTAGCTG	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28937_Mstr_Wil2021_COI	ADL-B17	1	BD28937_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	GTAGTGGTAACTACTCATGCTTTA	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28937_Pvir_Dia2013_COI	ADL-B17	1	BD28937_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	CTTAGTGGCATTAATTCGDAATCC	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28937_Rhar_Sim2018_COI	ADL-B17	1	BD28937_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	CTGCTGCTATTGCTCATG	138.50901
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28939_Aamu_Bax2006_COI	ADL-B15	1	BD28939_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	GCACAACCGGGATCTTTACTTC	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28939_Pcan_Bot2011_IGS	ADL-B15	1	BD28939_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B15	IGS	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	CGTAATCCTCAGTACTGGCTA	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28939_Pper_Dia2013_COI	ADL-B15	1	BD28939_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	CTTAGTGGCATTAATTCGDAATCC	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28939_Sspa_Oph2007_COI	ADL-B15	1	BD28939_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	GAAGGCCTTCCGATGATC	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28939_Upin_Bot2011_COI	ADL-B15	1	BD28939_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	CTTTAATTACAGCGTTTTTATTGTTGT	138.50806
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28940_Cmae_Bot2011_COI	ADL-B14	1	BD28940_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	ATGAACAGTCTATCCTCCTTTAG	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28940_Hsan_Wil2021_COI	ADL-B14	1	BD28940_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	CCTGGGCCGGTATAGTAGGT	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28940_Mros_Gib2022_COI	ADL-B14	1	BD28940_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28940_Msal_Bot2012_COI	ADL-B14	1	BD28940_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	GYTAGTTCCRATGATGTTAGCTG	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28940_Mstr_Wil2021_COI	ADL-B14	1	BD28940_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	GTAGTGGTAACTACTCATGCTTTA	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28940_Pvir_Dia2013_COI	ADL-B14	1	BD28940_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	CTTAGTGGCATTAATTCGDAATCC	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28940_Rhar_Sim2018_COI	ADL-B14	1	BD28940_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	CTGCTGCTATTGCTCATG	138.50873
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28942_Aamu_Bax2006_COI	ADL-B12	1	BD28942_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	GCACAACCGGGATCTTTACTTC	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28942_Pcan_Bot2011_IGS	ADL-B12	1	BD28942_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B12	IGS	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	CGTAATCCTCAGTACTGGCTA	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28942_Pper_Dia2013_COI	ADL-B12	1	BD28942_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	CTTAGTGGCATTAATTCGDAATCC	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28942_Sspa_Oph2007_COI	ADL-B12	1	BD28942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	GAAGGCCTTCCGATGATC	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28942_Upin_Bot2011_COI	ADL-B12	1	BD28942_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	CTTTAATTACAGCGTTTTTATTGTTGT	138.5093
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28943_Cjap_Sim2018_COI	ADL-B11	1	BD28943_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28943_Esin_And2018_Cyb	ADL-B11	1	BD28943_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B11	Cytb	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	ACCCCTCCTCATATCCAACCA	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.231	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28943_Mgig_Bot2012_COI	ADL-B11	1	BD28943_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	TCTTATTCGTTGGAGACTTTATAACCCT	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28943_Mjap_Gib2022_COI	ADL-B11	1	BD28943_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	AATTTAATTGTGACTAGGCATGGTCTG	138.50868
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28944_Cmae_Bot2011_COI	ADL-B10	1	BD28944_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	ATGAACAGTCTATCCTCCTTTAG	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28944_Hsan_Wil2021_COI	ADL-B10	1	BD28944_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	CCTGGGCCGGTATAGTAGGT	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28944_Mros_Gib2022_COI	ADL-B10	1	BD28944_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28944_Msal_Bot2012_COI	ADL-B10	1	BD28944_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	GYTAGTTCCRATGATGTTAGCTG	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28944_Mstr_Wil2021_COI	ADL-B10	1	BD28944_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	GTAGTGGTAACTACTCATGCTTTA	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28944_Pvir_Dia2013_COI	ADL-B10	1	BD28944_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	CTTAGTGGCATTAATTCGDAATCC	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28944_Rhar_Sim2018_COI	ADL-B10	1	BD28944_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	CTGCTGCTATTGCTCATG	138.50761
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28946_Aamu_Bax2006_COI	ADL-B08	1	BD28946_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	GCACAACCGGGATCTTTACTTC	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28946_Pcan_Bot2011_IGS	ADL-B08	1	BD28946_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B08	IGS	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	CGTAATCCTCAGTACTGGCTA	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28946_Pper_Dia2013_COI	ADL-B08	1	BD28946_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	CTTAGTGGCATTAATTCGDAATCC	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28946_Sspa_Oph2007_COI	ADL-B08	1	BD28946_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	GAAGGCCTTCCGATGATC	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28946_Upin_Bot2011_COI	ADL-B08	1	BD28946_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51028
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28947_Cjap_Sim2018_COI	ADL-B07	1	BD28947_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28947_Esin_And2018_Cyb	ADL-B07	1	BD28947_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B07	Cytb	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	ACCCCTCCTCATATCCAACCA	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.165	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28947_Mgig_Bot2012_COI	ADL-B07	1	BD28947_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	TCTTATTCGTTGGAGACTTTATAACCCT	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28947_Mjap_Gib2022_COI	ADL-B07	1	BD28947_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	AATTTAATTGTGACTAGGCATGGTCTG	138.50757
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28948_Cmae_Bot2011_COI	ADL-B06	1	BD28948_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	ATGAACAGTCTATCCTCCTTTAG	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28948_Hsan_Wil2021_COI	ADL-B06	1	BD28948_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	CCTGGGCCGGTATAGTAGGT	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28948_Mros_Gib2022_COI	ADL-B06	1	BD28948_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28948_Msal_Bot2012_COI	ADL-B06	1	BD28948_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	GYTAGTTCCRATGATGTTAGCTG	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28948_Mstr_Wil2021_COI	ADL-B06	1	BD28948_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	GTAGTGGTAACTACTCATGCTTTA	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28948_Pvir_Dia2013_COI	ADL-B06	1	BD28948_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	CTTAGTGGCATTAATTCGDAATCC	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28948_Rhar_Sim2018_COI	ADL-B06	1	BD28948_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	CTGCTGCTATTGCTCATG	138.50663
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28950_Cjap_Sim2018_COI	ADL-B04	1	BD28950_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28950_Esin_And2018_Cyb	ADL-B04	1	BD28950_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B04	Cytb	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	ACCCCTCCTCATATCCAACCA	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28950_Mgig_Bot2012_COI	ADL-B04	1	BD28950_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	TCTTATTCGTTGGAGACTTTATAACCCT	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28950_Mjap_Gib2022_COI	ADL-B04	1	BD28950_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	AATTTAATTGTGACTAGGCATGGTCTG	138.50603
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28951_Cmae_Bot2011_COI	ADL-B03	1	BD28951_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	ATGAACAGTCTATCCTCCTTTAG	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28951_Hsan_Wil2021_COI	ADL-B03	1	BD28951_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	CCTGGGCCGGTATAGTAGGT	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28951_Mros_Gib2022_COI	ADL-B03	1	BD28951_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28951_Msal_Bot2012_COI	ADL-B03	1	BD28951_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	GYTAGTTCCRATGATGTTAGCTG	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28951_Mstr_Wil2021_COI	ADL-B03	1	BD28951_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	GTAGTGGTAACTACTCATGCTTTA	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28951_Pvir_Dia2013_COI	ADL-B03	1	BD28951_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	CTTAGTGGCATTAATTCGDAATCC	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28951_Rhar_Sim2018_COI	ADL-B03	1	BD28951_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	CTGCTGCTATTGCTCATG	138.50558
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28953_Aamu_Bax2006_COI	ADL-B01	1	BD28953_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	GCACAACCGGGATCTTTACTTC	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28953_Pcan_Bot2011_IGS	ADL-B01	1	BD28953_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B01	IGS	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	CGTAATCCTCAGTACTGGCTA	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28953_Pper_Dia2013_COI	ADL-B01	1	BD28953_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	CTTAGTGGCATTAATTCGDAATCC	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28953_Sspa_Oph2007_COI	ADL-B01	1	BD28953_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	GAAGGCCTTCCGATGATC	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28953_Upin_Bot2011_COI	ADL-B01	1	BD28953_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	CTTTAATTACAGCGTTTTTATTGTTGT	138.49924
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28954_Cjap_Sim2018_COI	ADL-B70	1	BD28954_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28954_Esin_And2018_Cyb	ADL-B70	1	BD28954_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B70	Cytb	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	ACCCCTCCTCATATCCAACCA	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.753	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28954_Mgig_Bot2012_COI	ADL-B70	1	BD28954_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	TCTTATTCGTTGGAGACTTTATAACCCT	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28954_Mjap_Gib2022_COI	ADL-B70	1	BD28954_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	AATTTAATTGTGACTAGGCATGGTCTG	138.5006
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28955_Cmae_Bot2011_COI	ADL-B69	1	BD28955_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	ATGAACAGTCTATCCTCCTTTAG	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28955_Hsan_Wil2021_COI	ADL-B69	1	BD28955_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	CCTGGGCCGGTATAGTAGGT	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28955_Mros_Gib2022_COI	ADL-B69	1	BD28955_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28955_Msal_Bot2012_COI	ADL-B69	1	BD28955_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	GYTAGTTCCRATGATGTTAGCTG	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28955_Mstr_Wil2021_COI	ADL-B69	1	BD28955_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	GTAGTGGTAACTACTCATGCTTTA	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28955_Pvir_Dia2013_COI	ADL-B69	1	BD28955_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	CTTAGTGGCATTAATTCGDAATCC	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28955_Rhar_Sim2018_COI	ADL-B69	1	BD28955_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	CTGCTGCTATTGCTCATG	138.49891
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28957_Aamu_Bax2006_COI	ADL-B67	1	BD28957_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	GCACAACCGGGATCTTTACTTC	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28957_Pcan_Bot2011_IGS	ADL-B67	1	BD28957_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B67	IGS	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	CGTAATCCTCAGTACTGGCTA	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28957_Pper_Dia2013_COI	ADL-B67	1	BD28957_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	CTTAGTGGCATTAATTCGDAATCC	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28957_Sspa_Oph2007_COI	ADL-B67	1	BD28957_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	GAAGGCCTTCCGATGATC	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28957_Upin_Bot2011_COI	ADL-B67	1	BD28957_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	CTTTAATTACAGCGTTTTTATTGTTGT	138.49964
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28958_Cjap_Sim2018_COI	ADL-B66	1	BD28958_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28958_Esin_And2018_Cyb	ADL-B66	1	BD28958_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B66	Cytb	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	ACCCCTCCTCATATCCAACCA	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.516	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28958_Mgig_Bot2012_COI	ADL-B66	1	BD28958_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	TCTTATTCGTTGGAGACTTTATAACCCT	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28958_Mjap_Gib2022_COI	ADL-B66	1	BD28958_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	AATTTAATTGTGACTAGGCATGGTCTG	138.49752
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28959_Cmae_Bot2011_COI	ADL-B65	1	BD28959_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	ATGAACAGTCTATCCTCCTTTAG	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28959_Hsan_Wil2021_COI	ADL-B65	1	BD28959_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	CCTGGGCCGGTATAGTAGGT	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28959_Mros_Gib2022_COI	ADL-B65	1	BD28959_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28959_Msal_Bot2012_COI	ADL-B65	1	BD28959_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	GYTAGTTCCRATGATGTTAGCTG	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28959_Mstr_Wil2021_COI	ADL-B65	1	BD28959_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	GTAGTGGTAACTACTCATGCTTTA	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28959_Pvir_Dia2013_COI	ADL-B65	1	BD28959_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	CTTAGTGGCATTAATTCGDAATCC	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28959_Rhar_Sim2018_COI	ADL-B65	1	BD28959_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	CTGCTGCTATTGCTCATG	138.49536
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28960_Aamu_Bax2006_COI	ADL-B64	1	BD28960_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	GCACAACCGGGATCTTTACTTC	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28960_Pcan_Bot2011_IGS	ADL-B64	1	BD28960_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B64	IGS	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	CGTAATCCTCAGTACTGGCTA	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28960_Pper_Dia2013_COI	ADL-B64	1	BD28960_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	CTTAGTGGCATTAATTCGDAATCC	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.045	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28960_Sspa_Oph2007_COI	ADL-B64	1	BD28960_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	GAAGGCCTTCCGATGATC	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28960_Upin_Bot2011_COI	ADL-B64	1	BD28960_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	CTTTAATTACAGCGTTTTTATTGTTGT	138.49652
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28961_Cjap_Sim2018_COI	ADL-B63	1	BD28961_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28961_Esin_And2018_Cyb	ADL-B63	1	BD28961_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B63	Cytb	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	ACCCCTCCTCATATCCAACCA	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.358	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28961_Mgig_Bot2012_COI	ADL-B63	1	BD28961_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	TCTTATTCGTTGGAGACTTTATAACCCT	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28961_Mjap_Gib2022_COI	ADL-B63	1	BD28961_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	AATTTAATTGTGACTAGGCATGGTCTG	138.49468
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28962_Cmae_Bot2011_COI	ADL-B62	1	BD28962_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	ATGAACAGTCTATCCTCCTTTAG	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28962_Hsan_Wil2021_COI	ADL-B62	1	BD28962_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	CCTGGGCCGGTATAGTAGGT	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28962_Mros_Gib2022_COI	ADL-B62	1	BD28962_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28962_Msal_Bot2012_COI	ADL-B62	1	BD28962_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	GYTAGTTCCRATGATGTTAGCTG	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28962_Mstr_Wil2021_COI	ADL-B62	1	BD28962_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	GTAGTGGTAACTACTCATGCTTTA	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28962_Pvir_Dia2013_COI	ADL-B62	1	BD28962_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	CTTAGTGGCATTAATTCGDAATCC	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28962_Rhar_Sim2018_COI	ADL-B62	1	BD28962_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	CTGCTGCTATTGCTCATG	138.49227
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28964_Aamu_Bax2006_COI	ADL-B60	1	BD28964_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	GCACAACCGGGATCTTTACTTC	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28964_Pcan_Bot2011_IGS	ADL-B60	1	BD28964_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B60	IGS	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	CGTAATCCTCAGTACTGGCTA	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28964_Pper_Dia2013_COI	ADL-B60	1	BD28964_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	CTTAGTGGCATTAATTCGDAATCC	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28964_Sspa_Oph2007_COI	ADL-B60	1	BD28964_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	GAAGGCCTTCCGATGATC	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28964_Upin_Bot2011_COI	ADL-B60	1	BD28964_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	CTTTAATTACAGCGTTTTTATTGTTGT	138.49128
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28965_Cjap_Sim2018_COI	ADL-B59	1	BD28965_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28965_Esin_And2018_Cyb	ADL-B59	1	BD28965_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B59	Cytb	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	ACCCCTCCTCATATCCAACCA	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.165	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28965_Mgig_Bot2012_COI	ADL-B59	1	BD28965_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	TCTTATTCGTTGGAGACTTTATAACCCT	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28965_Mjap_Gib2022_COI	ADL-B59	1	BD28965_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	AATTTAATTGTGACTAGGCATGGTCTG	138.49026
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28966_Cmae_Bot2011_COI	ADL-B58	1	BD28966_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	ATGAACAGTCTATCCTCCTTTAG	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28966_Hsan_Wil2021_COI	ADL-B58	1	BD28966_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	CCTGGGCCGGTATAGTAGGT	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28966_Mros_Gib2022_COI	ADL-B58	1	BD28966_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28966_Msal_Bot2012_COI	ADL-B58	1	BD28966_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	GYTAGTTCCRATGATGTTAGCTG	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28966_Mstr_Wil2021_COI	ADL-B58	1	BD28966_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	GTAGTGGTAACTACTCATGCTTTA	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28966_Pvir_Dia2013_COI	ADL-B58	1	BD28966_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	CTTAGTGGCATTAATTCGDAATCC	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28966_Rhar_Sim2018_COI	ADL-B58	1	BD28966_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	CTGCTGCTATTGCTCATG	138.48822
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28968_Aamu_Bax2006_COI	ADL-B56	1	BD28968_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	GCACAACCGGGATCTTTACTTC	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28968_Pcan_Bot2011_IGS	ADL-B56	1	BD28968_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B56	IGS	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	CGTAATCCTCAGTACTGGCTA	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28968_Pper_Dia2013_COI	ADL-B56	1	BD28968_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	CTTAGTGGCATTAATTCGDAATCC	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28968_Sspa_Oph2007_COI	ADL-B56	1	BD28968_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	GAAGGCCTTCCGATGATC	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28968_Upin_Bot2011_COI	ADL-B56	1	BD28968_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	CTTTAATTACAGCGTTTTTATTGTTGT	138.48611
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28969_Cjap_Sim2018_COI	ADL-B55	1	BD28969_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28969_Esin_And2018_Cyb	ADL-B55	1	BD28969_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B55	Cytb	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	ACCCCTCCTCATATCCAACCA	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.728	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28969_Mgig_Bot2012_COI	ADL-B55	1	BD28969_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	TCTTATTCGTTGGAGACTTTATAACCCT	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28969_Mjap_Gib2022_COI	ADL-B55	1	BD28969_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	AATTTAATTGTGACTAGGCATGGTCTG	138.48937
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28971_Aamu_Bax2006_COI	ADL-B53	1	BD28971_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	GCACAACCGGGATCTTTACTTC	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28971_Pcan_Bot2011_IGS	ADL-B53	1	BD28971_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B53	IGS	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	CGTAATCCTCAGTACTGGCTA	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28971_Pper_Dia2013_COI	ADL-B53	1	BD28971_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	CTTAGTGGCATTAATTCGDAATCC	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28971_Sspa_Oph2007_COI	ADL-B53	1	BD28971_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	GAAGGCCTTCCGATGATC	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28971_Upin_Bot2011_COI	ADL-B53	1	BD28971_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	CTTTAATTACAGCGTTTTTATTGTTGT	138.48516
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28972_Cjap_Sim2018_COI	ADL-B52	1	BD28972_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28972_Esin_And2018_Cyb	ADL-B52	1	BD28972_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B52	Cytb	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	ACCCCTCCTCATATCCAACCA	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.012	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28972_Mgig_Bot2012_COI	ADL-B52	1	BD28972_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	TCTTATTCGTTGGAGACTTTATAACCCT	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28972_Mjap_Gib2022_COI	ADL-B52	1	BD28972_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	AATTTAATTGTGACTAGGCATGGTCTG	138.4862
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28973_Cmae_Bot2011_COI	ADL-B51	1	BD28973_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	ATGAACAGTCTATCCTCCTTTAG	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28973_Hsan_Wil2021_COI	ADL-B51	1	BD28973_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	CCTGGGCCGGTATAGTAGGT	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28973_Mros_Gib2022_COI	ADL-B51	1	BD28973_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28973_Msal_Bot2012_COI	ADL-B51	1	BD28973_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	GYTAGTTCCRATGATGTTAGCTG	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28973_Mstr_Wil2021_COI	ADL-B51	1	BD28973_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	GTAGTGGTAACTACTCATGCTTTA	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28973_Pvir_Dia2013_COI	ADL-B51	1	BD28973_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	CTTAGTGGCATTAATTCGDAATCC	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28973_Rhar_Sim2018_COI	ADL-B51	1	BD28973_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	CTGCTGCTATTGCTCATG	138.48404
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28975_Aamu_Bax2006_COI	ADL-B49	1	BD28975_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	GCACAACCGGGATCTTTACTTC	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28975_Pcan_Bot2011_IGS	ADL-B49	1	BD28975_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B49	IGS	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	CGTAATCCTCAGTACTGGCTA	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28975_Pper_Dia2013_COI	ADL-B49	1	BD28975_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	CTTAGTGGCATTAATTCGDAATCC	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	44.161	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28975_Sspa_Oph2007_COI	ADL-B49	1	BD28975_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	GAAGGCCTTCCGATGATC	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28975_Upin_Bot2011_COI	ADL-B49	1	BD28975_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	CTTTAATTACAGCGTTTTTATTGTTGT	138.48311
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28976_Cjap_Sim2018_COI	ADL-B48	1	BD28976_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28976_Esin_And2018_Cyb	ADL-B48	1	BD28976_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B48	Cytb	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	ACCCCTCCTCATATCCAACCA	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.669	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28976_Mgig_Bot2012_COI	ADL-B48	1	BD28976_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	TCTTATTCGTTGGAGACTTTATAACCCT	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28976_Mjap_Gib2022_COI	ADL-B48	1	BD28976_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	AATTTAATTGTGACTAGGCATGGTCTG	138.48421
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28977_Cmae_Bot2011_COI	ADL-B47	1	BD28977_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	ATGAACAGTCTATCCTCCTTTAG	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28977_Hsan_Wil2021_COI	ADL-B47	1	BD28977_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	CCTGGGCCGGTATAGTAGGT	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28977_Mros_Gib2022_COI	ADL-B47	1	BD28977_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28977_Msal_Bot2012_COI	ADL-B47	1	BD28977_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	GYTAGTTCCRATGATGTTAGCTG	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28977_Mstr_Wil2021_COI	ADL-B47	1	BD28977_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	GTAGTGGTAACTACTCATGCTTTA	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28977_Pvir_Dia2013_COI	ADL-B47	1	BD28977_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	CTTAGTGGCATTAATTCGDAATCC	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28977_Rhar_Sim2018_COI	ADL-B47	1	BD28977_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	CTGCTGCTATTGCTCATG	138.48203
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28979_Aamu_Bax2006_COI	ADL-B45	1	BD28979_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	GCACAACCGGGATCTTTACTTC	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28979_Pcan_Bot2011_IGS	ADL-B45	1	BD28979_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B45	IGS	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	CGTAATCCTCAGTACTGGCTA	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28979_Pper_Dia2013_COI	ADL-B45	1	BD28979_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	CTTAGTGGCATTAATTCGDAATCC	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28979_Sspa_Oph2007_COI	ADL-B45	1	BD28979_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	GAAGGCCTTCCGATGATC	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28979_Upin_Bot2011_COI	ADL-B45	1	BD28979_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	CTTTAATTACAGCGTTTTTATTGTTGT	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77618	WGS84	BD28979	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28979_Vgib_Wil2025_COI	ADL-B45	1	BD28979_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	CCCCTCTTTACAAGTTCTTTAGGGT	138.48234
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28980_Cmae_Bot2011_COI	ADL-B44	1	BD28980_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	ATGAACAGTCTATCCTCCTTTAG	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28980_Hsan_Wil2021_COI	ADL-B44	1	BD28980_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	CCTGGGCCGGTATAGTAGGT	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28980_Mros_Gib2022_COI	ADL-B44	1	BD28980_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28980_Msal_Bot2012_COI	ADL-B44	1	BD28980_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	GYTAGTTCCRATGATGTTAGCTG	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28980_Mstr_Wil2021_COI	ADL-B44	1	BD28980_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	GTAGTGGTAACTACTCATGCTTTA	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28980_Pvir_Dia2013_COI	ADL-B44	1	BD28980_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	CTTAGTGGCATTAATTCGDAATCC	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28980_Rhar_Sim2018_COI	ADL-B44	1	BD28980_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	CTGCTGCTATTGCTCATG	138.48112
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28982_Aamu_Bax2006_COI	ADL-B42	1	BD28982_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	GCACAACCGGGATCTTTACTTC	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28982_Pcan_Bot2011_IGS	ADL-B42	1	BD28982_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B42	IGS	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	CGTAATCCTCAGTACTGGCTA	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28982_Pper_Dia2013_COI	ADL-B42	1	BD28982_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	CTTAGTGGCATTAATTCGDAATCC	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28982_Sspa_Oph2007_COI	ADL-B42	1	BD28982_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	GAAGGCCTTCCGATGATC	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28982_Upin_Bot2011_COI	ADL-B42	1	BD28982_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	CTTTAATTACAGCGTTTTTATTGTTGT	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77789	WGS84	BD28982	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28982_Vgib_Wil2025_COI	ADL-B42	1	BD28982_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	CCCCTCTTTACAAGTTCTTTAGGGT	138.47916
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.7795	WGS84	BD28983	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28983_Asen_Wil2025_COI	ADL-B41	1	BD28983_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	GTTGTTGTTACAACACATGCTCTTTTG	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28983_Cjap_Sim2018_COI	ADL-B41	1	BD28983_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28983_Esin_And2018_Cyb	ADL-B41	1	BD28983_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B41	Cytb	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	ACCCCTCCTCATATCCAACCA	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28983_Mgig_Bot2012_COI	ADL-B41	1	BD28983_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	TCTTATTCGTTGGAGACTTTATAACCCT	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28983_Mjap_Gib2022_COI	ADL-B41	1	BD28983_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	AATTTAATTGTGACTAGGCATGGTCTG	138.47823
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28984_Cmae_Bot2011_COI	ADL-B40	1	BD28984_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	ATGAACAGTCTATCCTCCTTTAG	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28984_Hsan_Wil2021_COI	ADL-B40	1	BD28984_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	CCTGGGCCGGTATAGTAGGT	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28984_Mros_Gib2022_COI	ADL-B40	1	BD28984_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28984_Msal_Bot2012_COI	ADL-B40	1	BD28984_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	GYTAGTTCCRATGATGTTAGCTG	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28984_Mstr_Wil2021_COI	ADL-B40	1	BD28984_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	GTAGTGGTAACTACTCATGCTTTA	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28984_Pvir_Dia2013_COI	ADL-B40	1	BD28984_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	CTTAGTGGCATTAATTCGDAATCC	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28984_Rhar_Sim2018_COI	ADL-B40	1	BD28984_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	CTGCTGCTATTGCTCATG	138.47714
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28986_Aamu_Bax2006_COI	ADL-B38	1	BD28986_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	GCACAACCGGGATCTTTACTTC	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28986_Pcan_Bot2011_IGS	ADL-B38	1	BD28986_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B38	IGS	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	CGTAATCCTCAGTACTGGCTA	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28986_Pper_Dia2013_COI	ADL-B38	1	BD28986_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	CTTAGTGGCATTAATTCGDAATCC	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28986_Sspa_Oph2007_COI	ADL-B38	1	BD28986_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	GAAGGCCTTCCGATGATC	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28986_Upin_Bot2011_COI	ADL-B38	1	BD28986_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	CTTTAATTACAGCGTTTTTATTGTTGT	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78276	WGS84	BD28986	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28986_Vgib_Wil2025_COI	ADL-B38	1	BD28986_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	CCCCTCTTTACAAGTTCTTTAGGGT	138.47417
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78416	WGS84	BD28987	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28987_Asen_Wil2025_COI	ADL-B37	1	BD28987_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	GTTGTTGTTACAACACATGCTCTTTTG	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28987_Cjap_Sim2018_COI	ADL-B37	1	BD28987_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28987_Esin_And2018_Cyb	ADL-B37	1	BD28987_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B37	Cytb	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	ACCCCTCCTCATATCCAACCA	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.039	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28987_Mgig_Bot2012_COI	ADL-B37	1	BD28987_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	TCTTATTCGTTGGAGACTTTATAACCCT	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28987_Mjap_Gib2022_COI	ADL-B37	1	BD28987_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	AATTTAATTGTGACTAGGCATGGTCTG	138.47512
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28988_Cmae_Bot2011_COI	ADL-B36	1	BD28988_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	ATGAACAGTCTATCCTCCTTTAG	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28988_Hsan_Wil2021_COI	ADL-B36	1	BD28988_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	CCTGGGCCGGTATAGTAGGT	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28988_Mros_Gib2022_COI	ADL-B36	1	BD28988_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28988_Msal_Bot2012_COI	ADL-B36	1	BD28988_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	GYTAGTTCCRATGATGTTAGCTG	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28988_Mstr_Wil2021_COI	ADL-B36	1	BD28988_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	GTAGTGGTAACTACTCATGCTTTA	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28988_Pvir_Dia2013_COI	ADL-B36	1	BD28988_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	CTTAGTGGCATTAATTCGDAATCC	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28988_Rhar_Sim2018_COI	ADL-B36	1	BD28988_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	CTGCTGCTATTGCTCATG	138.47309
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28990_Cjap_Sim2018_COI	PLO-B39	1	BD28990_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28990_Esin_And2018_Cyb	PLO-B39	1	BD28990_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B39	Cytb	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	ACCCCTCCTCATATCCAACCA	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28990_Mgig_Bot2012_COI	PLO-B39	1	BD28990_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	TCTTATTCGTTGGAGACTTTATAACCCT	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28990_Mjap_Gib2022_COI	PLO-B39	1	BD28990_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	AATTTAATTGTGACTAGGCATGGTCTG	135.86996
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28991_Cmae_Bot2011_COI	PLO-B38	1	BD28991_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	ATGAACAGTCTATCCTCCTTTAG	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28991_Hsan_Wil2021_COI	PLO-B38	1	BD28991_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	CCTGGGCCGGTATAGTAGGT	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28991_Mros_Gib2022_COI	PLO-B38	1	BD28991_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28991_Msal_Bot2012_COI	PLO-B38	1	BD28991_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	GYTAGTTCCRATGATGTTAGCTG	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28991_Mstr_Wil2021_COI	PLO-B38	1	BD28991_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	GTAGTGGTAACTACTCATGCTTTA	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28991_Pvir_Dia2013_COI	PLO-B38	1	BD28991_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	CTTAGTGGCATTAATTCGDAATCC	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28991_Rhar_Sim2018_COI	PLO-B38	1	BD28991_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	CTGCTGCTATTGCTCATG	135.87269
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28993_Aamu_Bax2006_COI	PLO-B36	1	BD28993_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	GCACAACCGGGATCTTTACTTC	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28993_Pcan_Bot2011_IGS	PLO-B36	1	BD28993_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B36	IGS	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	CGTAATCCTCAGTACTGGCTA	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28993_Pper_Dia2013_COI	PLO-B36	1	BD28993_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	CTTAGTGGCATTAATTCGDAATCC	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28993_Sspa_Oph2007_COI	PLO-B36	1	BD28993_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	GAAGGCCTTCCGATGATC	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28993_Upin_Bot2011_COI	PLO-B36	1	BD28993_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	CTTTAATTACAGCGTTTTTATTGTTGT	135.87749
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28994_Cjap_Sim2018_COI	THE-B35	1	BD28994_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28994_Esin_And2018_Cyb	THE-B35	1	BD28994_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B35	Cytb	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	ACCCCTCCTCATATCCAACCA	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28994_Mgig_Bot2012_COI	THE-B35	1	BD28994_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	TCTTATTCGTTGGAGACTTTATAACCCT	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28994_Mjap_Gib2022_COI	THE-B35	1	BD28994_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	AATTTAATTGTGACTAGGCATGGTCTG	133.63829
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28995_Cmae_Bot2011_COI	THE-B34	1	BD28995_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	ATGAACAGTCTATCCTCCTTTAG	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28995_Hsan_Wil2021_COI	THE-B34	1	BD28995_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	CCTGGGCCGGTATAGTAGGT	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28995_Mros_Gib2022_COI	THE-B34	1	BD28995_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28995_Msal_Bot2012_COI	THE-B34	1	BD28995_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	GYTAGTTCCRATGATGTTAGCTG	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28995_Mstr_Wil2021_COI	THE-B34	1	BD28995_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	GTAGTGGTAACTACTCATGCTTTA	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28995_Pvir_Dia2013_COI	THE-B34	1	BD28995_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	CTTAGTGGCATTAATTCGDAATCC	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28995_Rhar_Sim2018_COI	THE-B34	1	BD28995_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	CTGCTGCTATTGCTCATG	133.63995
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28997_Aamu_Bax2006_COI	THE-B32	1	BD28997_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	GCACAACCGGGATCTTTACTTC	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28997_Pcan_Bot2011_IGS	THE-B32	1	BD28997_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B32	IGS	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	CGTAATCCTCAGTACTGGCTA	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28997_Pper_Dia2013_COI	THE-B32	1	BD28997_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	CTTAGTGGCATTAATTCGDAATCC	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28997_Sspa_Oph2007_COI	THE-B32	1	BD28997_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	GAAGGCCTTCCGATGATC	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28997_Upin_Bot2011_COI	THE-B32	1	BD28997_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	CTTTAATTACAGCGTTTTTATTGTTGT	133.63493
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28998_Cjap_Sim2018_COI	THE-B31	1	BD28998_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28998_Esin_And2018_Cyb	THE-B31	1	BD28998_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B31	Cytb	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	ACCCCTCCTCATATCCAACCA	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.717	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28998_Mgig_Bot2012_COI	THE-B31	1	BD28998_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	TCTTATTCGTTGGAGACTTTATAACCCT	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28998_Mjap_Gib2022_COI	THE-B31	1	BD28998_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	AATTTAATTGTGACTAGGCATGGTCTG	133.63652
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28999_Cmae_Bot2011_COI	THE-B30	1	BD28999_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	ATGAACAGTCTATCCTCCTTTAG	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28999_Hsan_Wil2021_COI	THE-B30	1	BD28999_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	CCTGGGCCGGTATAGTAGGT	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28999_Mros_Gib2022_COI	THE-B30	1	BD28999_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28999_Msal_Bot2012_COI	THE-B30	1	BD28999_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	GYTAGTTCCRATGATGTTAGCTG	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28999_Mstr_Wil2021_COI	THE-B30	1	BD28999_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	GTAGTGGTAACTACTCATGCTTTA	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28999_Pvir_Dia2013_COI	THE-B30	1	BD28999_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	CTTAGTGGCATTAATTCGDAATCC	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28999_Rhar_Sim2018_COI	THE-B30	1	BD28999_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	CTGCTGCTATTGCTCATG	133.63797
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29000_Aamu_Bax2006_COI	THE-B29	1	BD29000_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	GCACAACCGGGATCTTTACTTC	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29000_Pcan_Bot2011_IGS	THE-B29	1	BD29000_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B29	IGS	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	CGTAATCCTCAGTACTGGCTA	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29000_Pper_Dia2013_COI	THE-B29	1	BD29000_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	CTTAGTGGCATTAATTCGDAATCC	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29000_Sspa_Oph2007_COI	THE-B29	1	BD29000_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	GAAGGCCTTCCGATGATC	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29000_Upin_Bot2011_COI	THE-B29	1	BD29000_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	CTTTAATTACAGCGTTTTTATTGTTGT	133.6387
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29001_Cjap_Sim2018_COI	THE-B28	1	BD29001_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29001_Esin_And2018_Cyb	THE-B28	1	BD29001_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B28	Cytb	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	ACCCCTCCTCATATCCAACCA	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29001_Mgig_Bot2012_COI	THE-B28	1	BD29001_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	TCTTATTCGTTGGAGACTTTATAACCCT	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29001_Mjap_Gib2022_COI	THE-B28	1	BD29001_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	AATTTAATTGTGACTAGGCATGGTCTG	133.63921
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29002_Cmae_Bot2011_COI	THE-B27	1	BD29002_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	ATGAACAGTCTATCCTCCTTTAG	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29002_Hsan_Wil2021_COI	THE-B27	1	BD29002_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	CCTGGGCCGGTATAGTAGGT	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29002_Mros_Gib2022_COI	THE-B27	1	BD29002_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29002_Msal_Bot2012_COI	THE-B27	1	BD29002_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	GYTAGTTCCRATGATGTTAGCTG	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29002_Mstr_Wil2021_COI	THE-B27	1	BD29002_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	GTAGTGGTAACTACTCATGCTTTA	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29002_Pvir_Dia2013_COI	THE-B27	1	BD29002_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	CTTAGTGGCATTAATTCGDAATCC	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29002_Rhar_Sim2018_COI	THE-B27	1	BD29002_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	CTGCTGCTATTGCTCATG	133.64049
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29004_Aamu_Bax2006_COI	THE-B25	1	BD29004_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	GCACAACCGGGATCTTTACTTC	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29004_Pcan_Bot2011_IGS	THE-B25	1	BD29004_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B25	IGS	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	CGTAATCCTCAGTACTGGCTA	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29004_Pper_Dia2013_COI	THE-B25	1	BD29004_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	CTTAGTGGCATTAATTCGDAATCC	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29004_Sspa_Oph2007_COI	THE-B25	1	BD29004_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	GAAGGCCTTCCGATGATC	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29004_Upin_Bot2011_COI	THE-B25	1	BD29004_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	CTTTAATTACAGCGTTTTTATTGTTGT	133.63725
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29005_Cjap_Sim2018_COI	THE-B24	1	BD29005_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29005_Esin_And2018_Cyb	THE-B24	1	BD29005_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B24	Cytb	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	ACCCCTCCTCATATCCAACCA	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.449	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29005_Mgig_Bot2012_COI	THE-B24	1	BD29005_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	TCTTATTCGTTGGAGACTTTATAACCCT	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29005_Mjap_Gib2022_COI	THE-B24	1	BD29005_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	AATTTAATTGTGACTAGGCATGGTCTG	133.63886
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29006_Cmae_Bot2011_COI	THE-B23	1	BD29006_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	ATGAACAGTCTATCCTCCTTTAG	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29006_Hsan_Wil2021_COI	THE-B23	1	BD29006_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	CCTGGGCCGGTATAGTAGGT	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29006_Mros_Gib2022_COI	THE-B23	1	BD29006_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29006_Msal_Bot2012_COI	THE-B23	1	BD29006_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	GYTAGTTCCRATGATGTTAGCTG	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29006_Mstr_Wil2021_COI	THE-B23	1	BD29006_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	GTAGTGGTAACTACTCATGCTTTA	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29006_Pvir_Dia2013_COI	THE-B23	1	BD29006_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	CTTAGTGGCATTAATTCGDAATCC	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29006_Rhar_Sim2018_COI	THE-B23	1	BD29006_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	CTGCTGCTATTGCTCATG	133.64018
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29008_Aamu_Bax2006_COI	THE-B21	1	BD29008_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	GCACAACCGGGATCTTTACTTC	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29008_Pcan_Bot2011_IGS	THE-B21	1	BD29008_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B21	IGS	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	CGTAATCCTCAGTACTGGCTA	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29008_Pper_Dia2013_COI	THE-B21	1	BD29008_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	CTTAGTGGCATTAATTCGDAATCC	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29008_Sspa_Oph2007_COI	THE-B21	1	BD29008_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	GAAGGCCTTCCGATGATC	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29008_Upin_Bot2011_COI	THE-B21	1	BD29008_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	CTTTAATTACAGCGTTTTTATTGTTGT	133.63514
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29009_Cjap_Sim2018_COI	THE-B20	1	BD29009_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29009_Esin_And2018_Cyb	THE-B20	1	BD29009_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B20	Cytb	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	ACCCCTCCTCATATCCAACCA	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.176	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29009_Mgig_Bot2012_COI	THE-B20	1	BD29009_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	TCTTATTCGTTGGAGACTTTATAACCCT	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29009_Mjap_Gib2022_COI	THE-B20	1	BD29009_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	AATTTAATTGTGACTAGGCATGGTCTG	133.63656
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29011_Aamu_Bax2006_COI	THE-B18	1	BD29011_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	GCACAACCGGGATCTTTACTTC	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29011_Pcan_Bot2011_IGS	THE-B18	1	BD29011_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B18	IGS	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	CGTAATCCTCAGTACTGGCTA	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29011_Pper_Dia2013_COI	THE-B18	1	BD29011_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	CTTAGTGGCATTAATTCGDAATCC	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29011_Sspa_Oph2007_COI	THE-B18	1	BD29011_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	GAAGGCCTTCCGATGATC	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29011_Upin_Bot2011_COI	THE-B18	1	BD29011_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	CTTTAATTACAGCGTTTTTATTGTTGT	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15349	WGS84	BD29011	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29011_Vgib_Wil2025_COI	THE-B18	1	BD29011_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	CCCCTCTTTACAAGTTCTTTAGGGT	133.63787
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15352	WGS84	BD29012	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29012_Asen_Wil2025_COI	THE-B17	1	BD29012_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	GTTGTTGTTACAACACATGCTCTTTTG	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29012_Cjap_Sim2018_COI	THE-B17	1	BD29012_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29012_Esin_And2018_Cyb	THE-B17	1	BD29012_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B17	Cytb	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	ACCCCTCCTCATATCCAACCA	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.326	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29012_Mgig_Bot2012_COI	THE-B17	1	BD29012_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	TCTTATTCGTTGGAGACTTTATAACCCT	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29012_Mjap_Gib2022_COI	THE-B17	1	BD29012_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	AATTTAATTGTGACTAGGCATGGTCTG	133.63895
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29013_Cmae_Bot2011_COI	THE-B16	1	BD29013_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	ATGAACAGTCTATCCTCCTTTAG	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29013_Hsan_Wil2021_COI	THE-B16	1	BD29013_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	CCTGGGCCGGTATAGTAGGT	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29013_Mros_Gib2022_COI	THE-B16	1	BD29013_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29013_Msal_Bot2012_COI	THE-B16	1	BD29013_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	GYTAGTTCCRATGATGTTAGCTG	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29013_Mstr_Wil2021_COI	THE-B16	1	BD29013_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	GTAGTGGTAACTACTCATGCTTTA	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29013_Pvir_Dia2013_COI	THE-B16	1	BD29013_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	CTTAGTGGCATTAATTCGDAATCC	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29013_Rhar_Sim2018_COI	THE-B16	1	BD29013_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	CTGCTGCTATTGCTCATG	133.64047
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29015_Aamu_Bax2006_COI	THE-B14	1	BD29015_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	GCACAACCGGGATCTTTACTTC	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29015_Pcan_Bot2011_IGS	THE-B14	1	BD29015_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B14	IGS	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	CGTAATCCTCAGTACTGGCTA	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29015_Pper_Dia2013_COI	THE-B14	1	BD29015_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	CTTAGTGGCATTAATTCGDAATCC	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29015_Sspa_Oph2007_COI	THE-B14	1	BD29015_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	GAAGGCCTTCCGATGATC	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29015_Upin_Bot2011_COI	THE-B14	1	BD29015_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	CTTTAATTACAGCGTTTTTATTGTTGT	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15222	WGS84	BD29015	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29015_Vgib_Wil2025_COI	THE-B14	1	BD29015_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	CCCCTCTTTACAAGTTCTTTAGGGT	133.63854
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15214	WGS84	BD29016	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29016_Asen_Wil2025_COI	THE-B13	1	BD29016_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	GTTGTTGTTACAACACATGCTCTTTTG	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29016_Cjap_Sim2018_COI	THE-B13	1	BD29016_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29016_Esin_And2018_Cyb	THE-B13	1	BD29016_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B13	Cytb	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	ACCCCTCCTCATATCCAACCA	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	41.48	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29016_Mgig_Bot2012_COI	THE-B13	1	BD29016_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	TCTTATTCGTTGGAGACTTTATAACCCT	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29016_Mjap_Gib2022_COI	THE-B13	1	BD29016_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	AATTTAATTGTGACTAGGCATGGTCTG	133.63703
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29017_Cmae_Bot2011_COI	THE-B12	1	BD29017_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	ATGAACAGTCTATCCTCCTTTAG	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29017_Hsan_Wil2021_COI	THE-B12	1	BD29017_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	CCTGGGCCGGTATAGTAGGT	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29017_Mros_Gib2022_COI	THE-B12	1	BD29017_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29017_Msal_Bot2012_COI	THE-B12	1	BD29017_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	GYTAGTTCCRATGATGTTAGCTG	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29017_Mstr_Wil2021_COI	THE-B12	1	BD29017_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	GTAGTGGTAACTACTCATGCTTTA	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29017_Pvir_Dia2013_COI	THE-B12	1	BD29017_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	CTTAGTGGCATTAATTCGDAATCC	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29017_Rhar_Sim2018_COI	THE-B12	1	BD29017_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	CTGCTGCTATTGCTCATG	133.63559
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29019_Aamu_Bax2006_COI	THE-B10	1	BD29019_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	GCACAACCGGGATCTTTACTTC	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29019_Pcan_Bot2011_IGS	THE-B10	1	BD29019_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B10	IGS	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	CGTAATCCTCAGTACTGGCTA	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29019_Pper_Dia2013_COI	THE-B10	1	BD29019_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	CTTAGTGGCATTAATTCGDAATCC	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29019_Sspa_Oph2007_COI	THE-B10	1	BD29019_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	GAAGGCCTTCCGATGATC	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29019_Upin_Bot2011_COI	THE-B10	1	BD29019_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	CTTTAATTACAGCGTTTTTATTGTTGT	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15113	WGS84	BD29019	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29019_Vgib_Wil2025_COI	THE-B10	1	BD29019_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	CCCCTCTTTACAAGTTCTTTAGGGT	133.63644
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29020_Cmae_Bot2011_COI	THE-B09	1	BD29020_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	ATGAACAGTCTATCCTCCTTTAG	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29020_Hsan_Wil2021_COI	THE-B09	1	BD29020_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	CCTGGGCCGGTATAGTAGGT	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29020_Mros_Gib2022_COI	THE-B09	1	BD29020_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29020_Msal_Bot2012_COI	THE-B09	1	BD29020_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	GYTAGTTCCRATGATGTTAGCTG	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29020_Mstr_Wil2021_COI	THE-B09	1	BD29020_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	GTAGTGGTAACTACTCATGCTTTA	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29020_Pvir_Dia2013_COI	THE-B09	1	BD29020_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	CTTAGTGGCATTAATTCGDAATCC	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29020_Rhar_Sim2018_COI	THE-B09	1	BD29020_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	CTGCTGCTATTGCTCATG	133.63801
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29022_Aamu_Bax2006_COI	THE-B07	1	BD29022_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	GCACAACCGGGATCTTTACTTC	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29022_Pcan_Bot2011_IGS	THE-B07	1	BD29022_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B07	IGS	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	CGTAATCCTCAGTACTGGCTA	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29022_Pper_Dia2013_COI	THE-B07	1	BD29022_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	CTTAGTGGCATTAATTCGDAATCC	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29022_Sspa_Oph2007_COI	THE-B07	1	BD29022_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	GAAGGCCTTCCGATGATC	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29022_Upin_Bot2011_COI	THE-B07	1	BD29022_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	CTTTAATTACAGCGTTTTTATTGTTGT	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15125	WGS84	BD29022	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29022_Vgib_Wil2025_COI	THE-B07	1	BD29022_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	CCCCTCTTTACAAGTTCTTTAGGGT	133.64093
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15118	WGS84	BD29023	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29023_Asen_Wil2025_COI	THE-B06	1	BD29023_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	GTTGTTGTTACAACACATGCTCTTTTG	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29023_Cjap_Sim2018_COI	THE-B06	1	BD29023_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29023_Esin_And2018_Cyb	THE-B06	1	BD29023_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B06	Cytb	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	ACCCCTCCTCATATCCAACCA	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.708	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29023_Mgig_Bot2012_COI	THE-B06	1	BD29023_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	TCTTATTCGTTGGAGACTTTATAACCCT	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29023_Mjap_Gib2022_COI	THE-B06	1	BD29023_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	AATTTAATTGTGACTAGGCATGGTCTG	133.64194
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29024_Cmae_Bot2011_COI	THE-B05	1	BD29024_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	ATGAACAGTCTATCCTCCTTTAG	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29024_Hsan_Wil2021_COI	THE-B05	1	BD29024_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	CCTGGGCCGGTATAGTAGGT	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29024_Mros_Gib2022_COI	THE-B05	1	BD29024_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29024_Msal_Bot2012_COI	THE-B05	1	BD29024_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	GYTAGTTCCRATGATGTTAGCTG	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29024_Mstr_Wil2021_COI	THE-B05	1	BD29024_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	GTAGTGGTAACTACTCATGCTTTA	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29024_Pvir_Dia2013_COI	THE-B05	1	BD29024_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	CTTAGTGGCATTAATTCGDAATCC	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29024_Rhar_Sim2018_COI	THE-B05	1	BD29024_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	CTGCTGCTATTGCTCATG	133.64111
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29026_Aamu_Bax2006_COI	THE-B03	1	BD29026_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	GCACAACCGGGATCTTTACTTC	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29026_Pcan_Bot2011_IGS	THE-B03	1	BD29026_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B03	IGS	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	CGTAATCCTCAGTACTGGCTA	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29026_Pper_Dia2013_COI	THE-B03	1	BD29026_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	CTTAGTGGCATTAATTCGDAATCC	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29026_Sspa_Oph2007_COI	THE-B03	1	BD29026_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	GAAGGCCTTCCGATGATC	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29026_Upin_Bot2011_COI	THE-B03	1	BD29026_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	CTTTAATTACAGCGTTTTTATTGTTGT	133.63801
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29027_Cjap_Sim2018_COI	THE-B02	1	BD29027_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29027_Esin_And2018_Cyb	THE-B02	1	BD29027_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B02	Cytb	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	ACCCCTCCTCATATCCAACCA	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.617	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29027_Mgig_Bot2012_COI	THE-B02	1	BD29027_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	TCTTATTCGTTGGAGACTTTATAACCCT	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29027_Mjap_Gib2022_COI	THE-B02	1	BD29027_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	AATTTAATTGTGACTAGGCATGGTCTG	133.63706
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29028_Cmae_Bot2011_COI	THE-B01	1	BD29028_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	ATGAACAGTCTATCCTCCTTTAG	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29028_Hsan_Wil2021_COI	THE-B01	1	BD29028_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	CCTGGGCCGGTATAGTAGGT	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29028_Mros_Gib2022_COI	THE-B01	1	BD29028_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29028_Msal_Bot2012_COI	THE-B01	1	BD29028_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	GYTAGTTCCRATGATGTTAGCTG	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29028_Mstr_Wil2021_COI	THE-B01	1	BD29028_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	GTAGTGGTAACTACTCATGCTTTA	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29028_Pvir_Dia2013_COI	THE-B01	1	BD29028_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	CTTAGTGGCATTAATTCGDAATCC	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29028_Rhar_Sim2018_COI	THE-B01	1	BD29028_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	CTGCTGCTATTGCTCATG	133.63559
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29030_Cjap_Sim2018_COI	PGI-B34	1	BD29030_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29030_Esin_And2018_Cyb	PGI-B34	1	BD29030_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B34	Cytb	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	ACCCCTCCTCATATCCAACCA	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29030_Mgig_Bot2012_COI	PGI-B34	1	BD29030_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	TCTTATTCGTTGGAGACTTTATAACCCT	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29030_Mjap_Gib2022_COI	PGI-B34	1	BD29030_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	AATTTAATTGTGACTAGGCATGGTCTG	137.76884
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29031_Cmae_Bot2011_COI	PGI-B33	1	BD29031_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	ATGAACAGTCTATCCTCCTTTAG	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29031_Hsan_Wil2021_COI	PGI-B33	1	BD29031_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	CCTGGGCCGGTATAGTAGGT	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29031_Mros_Gib2022_COI	PGI-B33	1	BD29031_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29031_Msal_Bot2012_COI	PGI-B33	1	BD29031_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	GYTAGTTCCRATGATGTTAGCTG	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29031_Mstr_Wil2021_COI	PGI-B33	1	BD29031_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	GTAGTGGTAACTACTCATGCTTTA	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29031_Pvir_Dia2013_COI	PGI-B33	1	BD29031_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	CTTAGTGGCATTAATTCGDAATCC	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29031_Rhar_Sim2018_COI	PGI-B33	1	BD29031_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	CTGCTGCTATTGCTCATG	137.76708
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29033_Aamu_Bax2006_COI	PGI-B31	1	BD29033_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	GCACAACCGGGATCTTTACTTC	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29033_Pcan_Bot2011_IGS	PGI-B31	1	BD29033_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B31	IGS	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	CGTAATCCTCAGTACTGGCTA	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29033_Pper_Dia2013_COI	PGI-B31	1	BD29033_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	CTTAGTGGCATTAATTCGDAATCC	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29033_Sspa_Oph2007_COI	PGI-B31	1	BD29033_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	GAAGGCCTTCCGATGATC	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29033_Upin_Bot2011_COI	PGI-B31	1	BD29033_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	CTTTAATTACAGCGTTTTTATTGTTGT	137.76381
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29034_Cjap_Sim2018_COI	PGI-B30	1	BD29034_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29034_Esin_And2018_Cyb	PGI-B30	1	BD29034_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B30	Cytb	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	ACCCCTCCTCATATCCAACCA	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29034_Mgig_Bot2012_COI	PGI-B30	1	BD29034_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	TCTTATTCGTTGGAGACTTTATAACCCT	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29034_Mjap_Gib2022_COI	PGI-B30	1	BD29034_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	AATTTAATTGTGACTAGGCATGGTCTG	137.77147
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29035_Cmae_Bot2011_COI	PGI-B29	1	BD29035_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	ATGAACAGTCTATCCTCCTTTAG	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29035_Hsan_Wil2021_COI	PGI-B29	1	BD29035_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	CCTGGGCCGGTATAGTAGGT	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29035_Mros_Gib2022_COI	PGI-B29	1	BD29035_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29035_Msal_Bot2012_COI	PGI-B29	1	BD29035_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	GYTAGTTCCRATGATGTTAGCTG	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29035_Mstr_Wil2021_COI	PGI-B29	1	BD29035_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	GTAGTGGTAACTACTCATGCTTTA	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29035_Pvir_Dia2013_COI	PGI-B29	1	BD29035_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	CTTAGTGGCATTAATTCGDAATCC	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29035_Rhar_Sim2018_COI	PGI-B29	1	BD29035_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	CTGCTGCTATTGCTCATG	137.76983
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29037_Aamu_Bax2006_COI	PGI-B27	1	BD29037_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	GCACAACCGGGATCTTTACTTC	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29037_Pcan_Bot2011_IGS	PGI-B27	1	BD29037_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B27	IGS	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	CGTAATCCTCAGTACTGGCTA	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29037_Pper_Dia2013_COI	PGI-B27	1	BD29037_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	CTTAGTGGCATTAATTCGDAATCC	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29037_Sspa_Oph2007_COI	PGI-B27	1	BD29037_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	GAAGGCCTTCCGATGATC	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29037_Upin_Bot2011_COI	PGI-B27	1	BD29037_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	CTTTAATTACAGCGTTTTTATTGTTGT	137.7663
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29038_Cjap_Sim2018_COI	PGI-B26	1	BD29038_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29038_Esin_And2018_Cyb	PGI-B26	1	BD29038_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B26	Cytb	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	ACCCCTCCTCATATCCAACCA	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29038_Mgig_Bot2012_COI	PGI-B26	1	BD29038_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	TCTTATTCGTTGGAGACTTTATAACCCT	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29038_Mjap_Gib2022_COI	PGI-B26	1	BD29038_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	AATTTAATTGTGACTAGGCATGGTCTG	137.7646
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29039_Cmae_Bot2011_COI	PGI-B25	1	BD29039_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	ATGAACAGTCTATCCTCCTTTAG	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29039_Hsan_Wil2021_COI	PGI-B25	1	BD29039_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	CCTGGGCCGGTATAGTAGGT	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29039_Mros_Gib2022_COI	PGI-B25	1	BD29039_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29039_Msal_Bot2012_COI	PGI-B25	1	BD29039_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	GYTAGTTCCRATGATGTTAGCTG	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29039_Mstr_Wil2021_COI	PGI-B25	1	BD29039_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	GTAGTGGTAACTACTCATGCTTTA	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29039_Pvir_Dia2013_COI	PGI-B25	1	BD29039_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	CTTAGTGGCATTAATTCGDAATCC	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29039_Rhar_Sim2018_COI	PGI-B25	1	BD29039_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	CTGCTGCTATTGCTCATG	137.77239
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29040_Aamu_Bax2006_COI	PGI-B24	1	BD29040_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	GCACAACCGGGATCTTTACTTC	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29040_Pcan_Bot2011_IGS	PGI-B24	1	BD29040_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B24	IGS	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	CGTAATCCTCAGTACTGGCTA	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29040_Pper_Dia2013_COI	PGI-B24	1	BD29040_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	CTTAGTGGCATTAATTCGDAATCC	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29040_Sspa_Oph2007_COI	PGI-B24	1	BD29040_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	GAAGGCCTTCCGATGATC	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29040_Upin_Bot2011_COI	PGI-B24	1	BD29040_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	CTTTAATTACAGCGTTTTTATTGTTGT	137.77071
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29041_Cjap_Sim2018_COI	PGI-B23	1	BD29041_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29041_Esin_And2018_Cyb	PGI-B23	1	BD29041_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B23	Cytb	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	ACCCCTCCTCATATCCAACCA	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29041_Mgig_Bot2012_COI	PGI-B23	1	BD29041_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	TCTTATTCGTTGGAGACTTTATAACCCT	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29041_Mjap_Gib2022_COI	PGI-B23	1	BD29041_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	AATTTAATTGTGACTAGGCATGGTCTG	137.76897
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29042_Cmae_Bot2011_COI	PGI-B22	1	BD29042_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	ATGAACAGTCTATCCTCCTTTAG	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29042_Hsan_Wil2021_COI	PGI-B22	1	BD29042_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	CCTGGGCCGGTATAGTAGGT	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29042_Mros_Gib2022_COI	PGI-B22	1	BD29042_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29042_Msal_Bot2012_COI	PGI-B22	1	BD29042_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	GYTAGTTCCRATGATGTTAGCTG	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29042_Mstr_Wil2021_COI	PGI-B22	1	BD29042_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	GTAGTGGTAACTACTCATGCTTTA	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29042_Pvir_Dia2013_COI	PGI-B22	1	BD29042_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	CTTAGTGGCATTAATTCGDAATCC	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29042_Rhar_Sim2018_COI	PGI-B22	1	BD29042_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	CTGCTGCTATTGCTCATG	137.76718
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29044_Aamu_Bax2006_COI	PGI-B20	1	BD29044_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	GCACAACCGGGATCTTTACTTC	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29044_Pcan_Bot2011_IGS	PGI-B20	1	BD29044_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B20	IGS	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	CGTAATCCTCAGTACTGGCTA	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29044_Pper_Dia2013_COI	PGI-B20	1	BD29044_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	CTTAGTGGCATTAATTCGDAATCC	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29044_Sspa_Oph2007_COI	PGI-B20	1	BD29044_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	GAAGGCCTTCCGATGATC	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29044_Upin_Bot2011_COI	PGI-B20	1	BD29044_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	CTTTAATTACAGCGTTTTTATTGTTGT	137.76397
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29045_Cjap_Sim2018_COI	PGI-B19	1	BD29045_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29045_Esin_And2018_Cyb	PGI-B19	1	BD29045_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B19	Cytb	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	ACCCCTCCTCATATCCAACCA	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29045_Mgig_Bot2012_COI	PGI-B19	1	BD29045_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	TCTTATTCGTTGGAGACTTTATAACCCT	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29045_Mjap_Gib2022_COI	PGI-B19	1	BD29045_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	AATTTAATTGTGACTAGGCATGGTCTG	137.77334
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29046_Cmae_Bot2011_COI	PGI-B18	1	BD29046_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	ATGAACAGTCTATCCTCCTTTAG	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29046_Hsan_Wil2021_COI	PGI-B18	1	BD29046_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	CCTGGGCCGGTATAGTAGGT	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29046_Mros_Gib2022_COI	PGI-B18	1	BD29046_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29046_Msal_Bot2012_COI	PGI-B18	1	BD29046_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	GYTAGTTCCRATGATGTTAGCTG	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29046_Mstr_Wil2021_COI	PGI-B18	1	BD29046_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	GTAGTGGTAACTACTCATGCTTTA	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29046_Pvir_Dia2013_COI	PGI-B18	1	BD29046_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	CTTAGTGGCATTAATTCGDAATCC	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29046_Rhar_Sim2018_COI	PGI-B18	1	BD29046_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	CTGCTGCTATTGCTCATG	137.77165
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29048_Aamu_Bax2006_COI	PGI-B16	1	BD29048_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	GCACAACCGGGATCTTTACTTC	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29048_Pcan_Bot2011_IGS	PGI-B16	1	BD29048_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B16	IGS	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	CGTAATCCTCAGTACTGGCTA	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29048_Pper_Dia2013_COI	PGI-B16	1	BD29048_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	CTTAGTGGCATTAATTCGDAATCC	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29048_Sspa_Oph2007_COI	PGI-B16	1	BD29048_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	GAAGGCCTTCCGATGATC	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29048_Upin_Bot2011_COI	PGI-B16	1	BD29048_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	CTTTAATTACAGCGTTTTTATTGTTGT	137.77252
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29049_Cjap_Sim2018_COI	PGI-B15	1	BD29049_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29049_Esin_And2018_Cyb	PGI-B15	1	BD29049_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B15	Cytb	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	ACCCCTCCTCATATCCAACCA	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29049_Mgig_Bot2012_COI	PGI-B15	1	BD29049_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	TCTTATTCGTTGGAGACTTTATAACCCT	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29049_Mjap_Gib2022_COI	PGI-B15	1	BD29049_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	AATTTAATTGTGACTAGGCATGGTCTG	137.77077
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29051_Aamu_Bax2006_COI	PGI-B13	1	BD29051_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	GCACAACCGGGATCTTTACTTC	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29051_Pcan_Bot2011_IGS	PGI-B13	1	BD29051_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B13	IGS	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	CGTAATCCTCAGTACTGGCTA	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29051_Pper_Dia2013_COI	PGI-B13	1	BD29051_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	CTTAGTGGCATTAATTCGDAATCC	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29051_Sspa_Oph2007_COI	PGI-B13	1	BD29051_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	GAAGGCCTTCCGATGATC	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29051_Upin_Bot2011_COI	PGI-B13	1	BD29051_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	CTTTAATTACAGCGTTTTTATTGTTGT	137.7673
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29052_Cjap_Sim2018_COI	PGI-B12	1	BD29052_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29052_Esin_And2018_Cyb	PGI-B12	1	BD29052_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B12	Cytb	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	ACCCCTCCTCATATCCAACCA	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29052_Mgig_Bot2012_COI	PGI-B12	1	BD29052_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	TCTTATTCGTTGGAGACTTTATAACCCT	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29052_Mjap_Gib2022_COI	PGI-B12	1	BD29052_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	AATTTAATTGTGACTAGGCATGGTCTG	137.76561
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29053_Cmae_Bot2011_COI	PGI-B11	1	BD29053_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	ATGAACAGTCTATCCTCCTTTAG	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29053_Hsan_Wil2021_COI	PGI-B11	1	BD29053_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	CCTGGGCCGGTATAGTAGGT	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29053_Mros_Gib2022_COI	PGI-B11	1	BD29053_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29053_Msal_Bot2012_COI	PGI-B11	1	BD29053_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	GYTAGTTCCRATGATGTTAGCTG	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29053_Mstr_Wil2021_COI	PGI-B11	1	BD29053_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	GTAGTGGTAACTACTCATGCTTTA	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29053_Pvir_Dia2013_COI	PGI-B11	1	BD29053_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	CTTAGTGGCATTAATTCGDAATCC	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29053_Rhar_Sim2018_COI	PGI-B11	1	BD29053_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	CTGCTGCTATTGCTCATG	137.76396
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29055_Aamu_Bax2006_COI	PGI-B09	1	BD29055_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	GCACAACCGGGATCTTTACTTC	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29055_Pcan_Bot2011_IGS	PGI-B09	1	BD29055_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B09	IGS	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	CGTAATCCTCAGTACTGGCTA	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29055_Pper_Dia2013_COI	PGI-B09	1	BD29055_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	CTTAGTGGCATTAATTCGDAATCC	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29055_Sspa_Oph2007_COI	PGI-B09	1	BD29055_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	GAAGGCCTTCCGATGATC	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29055_Upin_Bot2011_COI	PGI-B09	1	BD29055_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	CTTTAATTACAGCGTTTTTATTGTTGT	137.76986
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29056_Cjap_Sim2018_COI	PGI-B08	1	BD29056_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29056_Esin_And2018_Cyb	PGI-B08	1	BD29056_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B08	Cytb	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	ACCCCTCCTCATATCCAACCA	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29056_Mgig_Bot2012_COI	PGI-B08	1	BD29056_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	TCTTATTCGTTGGAGACTTTATAACCCT	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29056_Mjap_Gib2022_COI	PGI-B08	1	BD29056_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	AATTTAATTGTGACTAGGCATGGTCTG	137.76814
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29057_Cmae_Bot2011_COI	PGI-B07	1	BD29057_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	ATGAACAGTCTATCCTCCTTTAG	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29057_Hsan_Wil2021_COI	PGI-B07	1	BD29057_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	CCTGGGCCGGTATAGTAGGT	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29057_Mros_Gib2022_COI	PGI-B07	1	BD29057_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29057_Msal_Bot2012_COI	PGI-B07	1	BD29057_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	GYTAGTTCCRATGATGTTAGCTG	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29057_Mstr_Wil2021_COI	PGI-B07	1	BD29057_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	GTAGTGGTAACTACTCATGCTTTA	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29057_Pvir_Dia2013_COI	PGI-B07	1	BD29057_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	CTTAGTGGCATTAATTCGDAATCC	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29057_Rhar_Sim2018_COI	PGI-B07	1	BD29057_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	CTGCTGCTATTGCTCATG	137.76643
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29059_Aamu_Bax2006_COI	PGI-B05	1	BD29059_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	GCACAACCGGGATCTTTACTTC	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29059_Pcan_Bot2011_IGS	PGI-B05	1	BD29059_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B05	IGS	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	CGTAATCCTCAGTACTGGCTA	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29059_Pper_Dia2013_COI	PGI-B05	1	BD29059_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	CTTAGTGGCATTAATTCGDAATCC	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29059_Sspa_Oph2007_COI	PGI-B05	1	BD29059_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	GAAGGCCTTCCGATGATC	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29059_Upin_Bot2011_COI	PGI-B05	1	BD29059_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	CTTTAATTACAGCGTTTTTATTGTTGT	137.77076
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29060_Cmae_Bot2011_COI	PGI-B04	1	BD29060_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	ATGAACAGTCTATCCTCCTTTAG	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29060_Hsan_Wil2021_COI	PGI-B04	1	BD29060_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	CCTGGGCCGGTATAGTAGGT	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29060_Mros_Gib2022_COI	PGI-B04	1	BD29060_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29060_Msal_Bot2012_COI	PGI-B04	1	BD29060_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	GYTAGTTCCRATGATGTTAGCTG	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29060_Mstr_Wil2021_COI	PGI-B04	1	BD29060_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	GTAGTGGTAACTACTCATGCTTTA	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29060_Pvir_Dia2013_COI	PGI-B04	1	BD29060_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	CTTAGTGGCATTAATTCGDAATCC	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29060_Rhar_Sim2018_COI	PGI-B04	1	BD29060_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	CTGCTGCTATTGCTCATG	137.76916
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29062_Aamu_Bax2006_COI	PGI-B02	1	BD29062_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	GCACAACCGGGATCTTTACTTC	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29062_Pcan_Bot2011_IGS	PGI-B02	1	BD29062_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B02	IGS	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	CGTAATCCTCAGTACTGGCTA	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29062_Pper_Dia2013_COI	PGI-B02	1	BD29062_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	CTTAGTGGCATTAATTCGDAATCC	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29062_Sspa_Oph2007_COI	PGI-B02	1	BD29062_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	GAAGGCCTTCCGATGATC	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29062_Upin_Bot2011_COI	PGI-B02	1	BD29062_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	CTTTAATTACAGCGTTTTTATTGTTGT	137.76554
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29063_Cjap_Sim2018_COI	PGI-B01	1	BD29063_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29063_Esin_And2018_Cyb	PGI-B01	1	BD29063_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B01	Cytb	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	ACCCCTCCTCATATCCAACCA	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29063_Mgig_Bot2012_COI	PGI-B01	1	BD29063_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	TCTTATTCGTTGGAGACTTTATAACCCT	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29063_Mjap_Gib2022_COI	PGI-B01	1	BD29063_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	AATTTAATTGTGACTAGGCATGGTCTG	137.76396
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29064_Cmae_Bot2011_COI	KPT-B35	1	BD29064_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	ATGAACAGTCTATCCTCCTTTAG	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29064_Hsan_Wil2021_COI	KPT-B35	1	BD29064_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	CCTGGGCCGGTATAGTAGGT	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29064_Mros_Gib2022_COI	KPT-B35	1	BD29064_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29064_Msal_Bot2012_COI	KPT-B35	1	BD29064_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	GYTAGTTCCRATGATGTTAGCTG	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29064_Mstr_Wil2021_COI	KPT-B35	1	BD29064_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	GTAGTGGTAACTACTCATGCTTTA	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29064_Pvir_Dia2013_COI	KPT-B35	1	BD29064_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	CTTAGTGGCATTAATTCGDAATCC	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29064_Rhar_Sim2018_COI	KPT-B35	1	BD29064_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	CTGCTGCTATTGCTCATG	137.78064
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29066_Aamu_Bax2006_COI	KPT-B33	1	BD29066_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	GCACAACCGGGATCTTTACTTC	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29066_Pcan_Bot2011_IGS	KPT-B33	1	BD29066_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B33	IGS	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	CGTAATCCTCAGTACTGGCTA	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29066_Pper_Dia2013_COI	KPT-B33	1	BD29066_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	CTTAGTGGCATTAATTCGDAATCC	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29066_Sspa_Oph2007_COI	KPT-B33	1	BD29066_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	GAAGGCCTTCCGATGATC	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29066_Upin_Bot2011_COI	KPT-B33	1	BD29066_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	CTTTAATTACAGCGTTTTTATTGTTGT	137.77795
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29067_Cjap_Sim2018_COI	KPT-B32	1	BD29067_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29067_Esin_And2018_Cyb	KPT-B32	1	BD29067_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B32	Cytb	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	ACCCCTCCTCATATCCAACCA	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29067_Mgig_Bot2012_COI	KPT-B32	1	BD29067_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	TCTTATTCGTTGGAGACTTTATAACCCT	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29067_Mjap_Gib2022_COI	KPT-B32	1	BD29067_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	AATTTAATTGTGACTAGGCATGGTCTG	137.77676
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29068_Cmae_Bot2011_COI	KPT-B31	1	BD29068_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	ATGAACAGTCTATCCTCCTTTAG	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29068_Hsan_Wil2021_COI	KPT-B31	1	BD29068_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	CCTGGGCCGGTATAGTAGGT	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29068_Mros_Gib2022_COI	KPT-B31	1	BD29068_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29068_Msal_Bot2012_COI	KPT-B31	1	BD29068_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	GYTAGTTCCRATGATGTTAGCTG	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29068_Mstr_Wil2021_COI	KPT-B31	1	BD29068_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	GTAGTGGTAACTACTCATGCTTTA	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29068_Pvir_Dia2013_COI	KPT-B31	1	BD29068_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	CTTAGTGGCATTAATTCGDAATCC	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29068_Rhar_Sim2018_COI	KPT-B31	1	BD29068_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	CTGCTGCTATTGCTCATG	137.77987
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29070_Cjap_Sim2018_COI	KPT-B29	1	BD29070_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29070_Esin_And2018_Cyb	KPT-B29	1	BD29070_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B29	Cytb	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	ACCCCTCCTCATATCCAACCA	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29070_Mgig_Bot2012_COI	KPT-B29	1	BD29070_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	TCTTATTCGTTGGAGACTTTATAACCCT	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29070_Mjap_Gib2022_COI	KPT-B29	1	BD29070_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	AATTTAATTGTGACTAGGCATGGTCTG	137.7774
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29071_Cmae_Bot2011_COI	KPT-B28	1	BD29071_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	ATGAACAGTCTATCCTCCTTTAG	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29071_Hsan_Wil2021_COI	KPT-B28	1	BD29071_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	CCTGGGCCGGTATAGTAGGT	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29071_Mros_Gib2022_COI	KPT-B28	1	BD29071_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29071_Msal_Bot2012_COI	KPT-B28	1	BD29071_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	GYTAGTTCCRATGATGTTAGCTG	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29071_Mstr_Wil2021_COI	KPT-B28	1	BD29071_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	GTAGTGGTAACTACTCATGCTTTA	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29071_Pvir_Dia2013_COI	KPT-B28	1	BD29071_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	CTTAGTGGCATTAATTCGDAATCC	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29071_Rhar_Sim2018_COI	KPT-B28	1	BD29071_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	CTGCTGCTATTGCTCATG	137.77599
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29073_Aamu_Bax2006_COI	KPT-B26	1	BD29073_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	GCACAACCGGGATCTTTACTTC	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29073_Pcan_Bot2011_IGS	KPT-B26	1	BD29073_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B26	IGS	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	CGTAATCCTCAGTACTGGCTA	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29073_Pper_Dia2013_COI	KPT-B26	1	BD29073_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	CTTAGTGGCATTAATTCGDAATCC	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29073_Sspa_Oph2007_COI	KPT-B26	1	BD29073_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	GAAGGCCTTCCGATGATC	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29073_Upin_Bot2011_COI	KPT-B26	1	BD29073_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	CTTTAATTACAGCGTTTTTATTGTTGT	137.77788
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29074_Cjap_Sim2018_COI	KPT-B25	1	BD29074_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29074_Esin_And2018_Cyb	KPT-B25	1	BD29074_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B25	Cytb	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	ACCCCTCCTCATATCCAACCA	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29074_Mgig_Bot2012_COI	KPT-B25	1	BD29074_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	TCTTATTCGTTGGAGACTTTATAACCCT	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29074_Mjap_Gib2022_COI	KPT-B25	1	BD29074_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	AATTTAATTGTGACTAGGCATGGTCTG	137.77663
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29075_Cmae_Bot2011_COI	KPT-B24	1	BD29075_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	ATGAACAGTCTATCCTCCTTTAG	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29075_Hsan_Wil2021_COI	KPT-B24	1	BD29075_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	CCTGGGCCGGTATAGTAGGT	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29075_Mros_Gib2022_COI	KPT-B24	1	BD29075_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29075_Msal_Bot2012_COI	KPT-B24	1	BD29075_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	GYTAGTTCCRATGATGTTAGCTG	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29075_Mstr_Wil2021_COI	KPT-B24	1	BD29075_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	GTAGTGGTAACTACTCATGCTTTA	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29075_Pvir_Dia2013_COI	KPT-B24	1	BD29075_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	CTTAGTGGCATTAATTCGDAATCC	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29075_Rhar_Sim2018_COI	KPT-B24	1	BD29075_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	CTGCTGCTATTGCTCATG	137.77851
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29077_Aamu_Bax2006_COI	KPT-B22	1	BD29077_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	GCACAACCGGGATCTTTACTTC	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29077_Pcan_Bot2011_IGS	KPT-B22	1	BD29077_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B22	IGS	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	CGTAATCCTCAGTACTGGCTA	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29077_Pper_Dia2013_COI	KPT-B22	1	BD29077_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	CTTAGTGGCATTAATTCGDAATCC	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29077_Sspa_Oph2007_COI	KPT-B22	1	BD29077_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	GAAGGCCTTCCGATGATC	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29077_Upin_Bot2011_COI	KPT-B22	1	BD29077_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	CTTTAATTACAGCGTTTTTATTGTTGT	137.77776
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29078_Cjap_Sim2018_COI	KPT-B21	1	BD29078_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29078_Esin_And2018_Cyb	KPT-B21	1	BD29078_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B21	Cytb	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	ACCCCTCCTCATATCCAACCA	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29078_Mgig_Bot2012_COI	KPT-B21	1	BD29078_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	TCTTATTCGTTGGAGACTTTATAACCCT	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29078_Mjap_Gib2022_COI	KPT-B21	1	BD29078_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	AATTTAATTGTGACTAGGCATGGTCTG	137.77675
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29079_Cmae_Bot2011_COI	KPT-B20	1	BD29079_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	ATGAACAGTCTATCCTCCTTTAG	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29079_Hsan_Wil2021_COI	KPT-B20	1	BD29079_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	CCTGGGCCGGTATAGTAGGT	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29079_Mros_Gib2022_COI	KPT-B20	1	BD29079_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29079_Msal_Bot2012_COI	KPT-B20	1	BD29079_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	GYTAGTTCCRATGATGTTAGCTG	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29079_Mstr_Wil2021_COI	KPT-B20	1	BD29079_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	GTAGTGGTAACTACTCATGCTTTA	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29079_Pvir_Dia2013_COI	KPT-B20	1	BD29079_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	CTTAGTGGCATTAATTCGDAATCC	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29079_Rhar_Sim2018_COI	KPT-B20	1	BD29079_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	CTGCTGCTATTGCTCATG	137.77984
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29080_Aamu_Bax2006_COI	KPT-B19	1	BD29080_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	GCACAACCGGGATCTTTACTTC	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29080_Pcan_Bot2011_IGS	KPT-B19	1	BD29080_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B19	IGS	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	CGTAATCCTCAGTACTGGCTA	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29080_Pper_Dia2013_COI	KPT-B19	1	BD29080_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	CTTAGTGGCATTAATTCGDAATCC	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29080_Sspa_Oph2007_COI	KPT-B19	1	BD29080_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	GAAGGCCTTCCGATGATC	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29080_Upin_Bot2011_COI	KPT-B19	1	BD29080_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	CTTTAATTACAGCGTTTTTATTGTTGT	137.77857
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29081_Cjap_Sim2018_COI	KPT-B18	1	BD29081_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29081_Esin_And2018_Cyb	KPT-B18	1	BD29081_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B18	Cytb	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	ACCCCTCCTCATATCCAACCA	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29081_Mgig_Bot2012_COI	KPT-B18	1	BD29081_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	TCTTATTCGTTGGAGACTTTATAACCCT	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29081_Mjap_Gib2022_COI	KPT-B18	1	BD29081_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	AATTTAATTGTGACTAGGCATGGTCTG	137.77734
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29082_Cmae_Bot2011_COI	KPT-B17	1	BD29082_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	ATGAACAGTCTATCCTCCTTTAG	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29082_Hsan_Wil2021_COI	KPT-B17	1	BD29082_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	CCTGGGCCGGTATAGTAGGT	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29082_Mros_Gib2022_COI	KPT-B17	1	BD29082_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29082_Msal_Bot2012_COI	KPT-B17	1	BD29082_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	GYTAGTTCCRATGATGTTAGCTG	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29082_Mstr_Wil2021_COI	KPT-B17	1	BD29082_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	GTAGTGGTAACTACTCATGCTTTA	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29082_Pvir_Dia2013_COI	KPT-B17	1	BD29082_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	CTTAGTGGCATTAATTCGDAATCC	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29082_Rhar_Sim2018_COI	KPT-B17	1	BD29082_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	CTGCTGCTATTGCTCATG	137.77611
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29084_Aamu_Bax2006_COI	KPT-B15	1	BD29084_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	GCACAACCGGGATCTTTACTTC	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29084_Pcan_Bot2011_IGS	KPT-B15	1	BD29084_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B15	IGS	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	CGTAATCCTCAGTACTGGCTA	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29084_Pper_Dia2013_COI	KPT-B15	1	BD29084_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	CTTAGTGGCATTAATTCGDAATCC	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29084_Sspa_Oph2007_COI	KPT-B15	1	BD29084_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	GAAGGCCTTCCGATGATC	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29084_Upin_Bot2011_COI	KPT-B15	1	BD29084_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	CTTTAATTACAGCGTTTTTATTGTTGT	137.77929
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29085_Cjap_Sim2018_COI	KPT-B14	1	BD29085_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29085_Esin_And2018_Cyb	KPT-B14	1	BD29085_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B14	Cytb	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	ACCCCTCCTCATATCCAACCA	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29085_Mgig_Bot2012_COI	KPT-B14	1	BD29085_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	TCTTATTCGTTGGAGACTTTATAACCCT	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29085_Mjap_Gib2022_COI	KPT-B14	1	BD29085_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	AATTTAATTGTGACTAGGCATGGTCTG	137.77798
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29086_Cmae_Bot2011_COI	KPT-B13	1	BD29086_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	ATGAACAGTCTATCCTCCTTTAG	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29086_Hsan_Wil2021_COI	KPT-B13	1	BD29086_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	CCTGGGCCGGTATAGTAGGT	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29086_Mros_Gib2022_COI	KPT-B13	1	BD29086_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29086_Msal_Bot2012_COI	KPT-B13	1	BD29086_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	GYTAGTTCCRATGATGTTAGCTG	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29086_Mstr_Wil2021_COI	KPT-B13	1	BD29086_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	GTAGTGGTAACTACTCATGCTTTA	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29086_Pvir_Dia2013_COI	KPT-B13	1	BD29086_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	CTTAGTGGCATTAATTCGDAATCC	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29086_Rhar_Sim2018_COI	KPT-B13	1	BD29086_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	CTGCTGCTATTGCTCATG	137.77686
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29088_Aamu_Bax2006_COI	KPT-B11	1	BD29088_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	GCACAACCGGGATCTTTACTTC	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29088_Pcan_Bot2011_IGS	KPT-B11	1	BD29088_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B11	IGS	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	CGTAATCCTCAGTACTGGCTA	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29088_Pper_Dia2013_COI	KPT-B11	1	BD29088_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	CTTAGTGGCATTAATTCGDAATCC	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29088_Sspa_Oph2007_COI	KPT-B11	1	BD29088_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	GAAGGCCTTCCGATGATC	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29088_Upin_Bot2011_COI	KPT-B11	1	BD29088_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	CTTTAATTACAGCGTTTTTATTGTTGT	137.77407
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29089_Cjap_Sim2018_COI	KPT-B10	1	BD29089_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29089_Esin_And2018_Cyb	KPT-B10	1	BD29089_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B10	Cytb	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	ACCCCTCCTCATATCCAACCA	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29089_Mgig_Bot2012_COI	KPT-B10	1	BD29089_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	TCTTATTCGTTGGAGACTTTATAACCCT	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29089_Mjap_Gib2022_COI	KPT-B10	1	BD29089_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	AATTTAATTGTGACTAGGCATGGTCTG	137.78008
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29091_Aamu_Bax2006_COI	KPT-B08	1	BD29091_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	GCACAACCGGGATCTTTACTTC	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29091_Pcan_Bot2011_IGS	KPT-B08	1	BD29091_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B08	IGS	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	CGTAATCCTCAGTACTGGCTA	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29091_Pper_Dia2013_COI	KPT-B08	1	BD29091_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	CTTAGTGGCATTAATTCGDAATCC	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29091_Sspa_Oph2007_COI	KPT-B08	1	BD29091_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	GAAGGCCTTCCGATGATC	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29091_Upin_Bot2011_COI	KPT-B08	1	BD29091_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	CTTTAATTACAGCGTTTTTATTGTTGT	137.77739
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29092_Cjap_Sim2018_COI	KPT-B07	1	BD29092_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29092_Esin_And2018_Cyb	KPT-B07	1	BD29092_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B07	Cytb	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	ACCCCTCCTCATATCCAACCA	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29092_Mgig_Bot2012_COI	KPT-B07	1	BD29092_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	TCTTATTCGTTGGAGACTTTATAACCCT	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29092_Mjap_Gib2022_COI	KPT-B07	1	BD29092_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	AATTTAATTGTGACTAGGCATGGTCTG	137.77614
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29093_Cmae_Bot2011_COI	KPT-B06	1	BD29093_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	ATGAACAGTCTATCCTCCTTTAG	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29093_Hsan_Wil2021_COI	KPT-B06	1	BD29093_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	CCTGGGCCGGTATAGTAGGT	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29093_Mros_Gib2022_COI	KPT-B06	1	BD29093_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29093_Msal_Bot2012_COI	KPT-B06	1	BD29093_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	GYTAGTTCCRATGATGTTAGCTG	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29093_Mstr_Wil2021_COI	KPT-B06	1	BD29093_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	GTAGTGGTAACTACTCATGCTTTA	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29093_Pvir_Dia2013_COI	KPT-B06	1	BD29093_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	CTTAGTGGCATTAATTCGDAATCC	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29093_Rhar_Sim2018_COI	KPT-B06	1	BD29093_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	CTGCTGCTATTGCTCATG	137.77479
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29095_Aamu_Bax2006_COI	KPT-B04	1	BD29095_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	GCACAACCGGGATCTTTACTTC	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29095_Pcan_Bot2011_IGS	KPT-B04	1	BD29095_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B04	IGS	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	CGTAATCCTCAGTACTGGCTA	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29095_Pper_Dia2013_COI	KPT-B04	1	BD29095_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	CTTAGTGGCATTAATTCGDAATCC	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29095_Sspa_Oph2007_COI	KPT-B04	1	BD29095_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	GAAGGCCTTCCGATGATC	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29095_Upin_Bot2011_COI	KPT-B04	1	BD29095_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	CTTTAATTACAGCGTTTTTATTGTTGT	137.77813
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29096_Cjap_Sim2018_COI	KPT-B03	1	BD29096_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29096_Esin_And2018_Cyb	KPT-B03	1	BD29096_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B03	Cytb	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	ACCCCTCCTCATATCCAACCA	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29096_Mgig_Bot2012_COI	KPT-B03	1	BD29096_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	TCTTATTCGTTGGAGACTTTATAACCCT	137.77684
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29097_Cmae_Bot2011_COI	KPT-B02	1	BD29097_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	ATGAACAGTCTATCCTCCTTTAG	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29097_Hsan_Wil2021_COI	KPT-B02	1	BD29097_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	CCTGGGCCGGTATAGTAGGT	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29097_Mros_Gib2022_COI	KPT-B02	1	BD29097_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29097_Msal_Bot2012_COI	KPT-B02	1	BD29097_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	GYTAGTTCCRATGATGTTAGCTG	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29097_Mstr_Wil2021_COI	KPT-B02	1	BD29097_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	GTAGTGGTAACTACTCATGCTTTA	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29097_Pvir_Dia2013_COI	KPT-B02	1	BD29097_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	CTTAGTGGCATTAATTCGDAATCC	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29097_Rhar_Sim2018_COI	KPT-B02	1	BD29097_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	CTGCTGCTATTGCTCATG	137.77554
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29099_Aamu_Bax2006_COI	PLO-B35	1	BD29099_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	GCACAACCGGGATCTTTACTTC	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29099_Pcan_Bot2011_IGS	PLO-B35	1	BD29099_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B35	IGS	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	CGTAATCCTCAGTACTGGCTA	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29099_Pper_Dia2013_COI	PLO-B35	1	BD29099_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	CTTAGTGGCATTAATTCGDAATCC	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29099_Sspa_Oph2007_COI	PLO-B35	1	BD29099_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	GAAGGCCTTCCGATGATC	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29099_Upin_Bot2011_COI	PLO-B35	1	BD29099_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	CTTTAATTACAGCGTTTTTATTGTTGT	135.87716
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29100_Cjap_Sim2018_COI	PLO-B34	1	BD29100_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29100_Esin_And2018_Cyb	PLO-B34	1	BD29100_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B34	Cytb	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	ACCCCTCCTCATATCCAACCA	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29100_Mgig_Bot2012_COI	PLO-B34	1	BD29100_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	TCTTATTCGTTGGAGACTTTATAACCCT	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29100_Mjap_Gib2022_COI	PLO-B34	1	BD29100_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	AATTTAATTGTGACTAGGCATGGTCTG	135.87284
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29101_Cmae_Bot2011_COI	PLO-B33	1	BD29101_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	ATGAACAGTCTATCCTCCTTTAG	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29101_Hsan_Wil2021_COI	PLO-B33	1	BD29101_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	CCTGGGCCGGTATAGTAGGT	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29101_Mros_Gib2022_COI	PLO-B33	1	BD29101_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29101_Msal_Bot2012_COI	PLO-B33	1	BD29101_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	GYTAGTTCCRATGATGTTAGCTG	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29101_Mstr_Wil2021_COI	PLO-B33	1	BD29101_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	GTAGTGGTAACTACTCATGCTTTA	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29101_Pvir_Dia2013_COI	PLO-B33	1	BD29101_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	CTTAGTGGCATTAATTCGDAATCC	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29101_Rhar_Sim2018_COI	PLO-B33	1	BD29101_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	CTGCTGCTATTGCTCATG	135.87046
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29103_Aamu_Bax2006_COI	PLO-B31	1	BD29103_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	GCACAACCGGGATCTTTACTTC	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29103_Pcan_Bot2011_IGS	PLO-B31	1	BD29103_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B31	IGS	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	CGTAATCCTCAGTACTGGCTA	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29103_Pper_Dia2013_COI	PLO-B31	1	BD29103_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	CTTAGTGGCATTAATTCGDAATCC	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29103_Sspa_Oph2007_COI	PLO-B31	1	BD29103_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	GAAGGCCTTCCGATGATC	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29103_Upin_Bot2011_COI	PLO-B31	1	BD29103_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	CTTTAATTACAGCGTTTTTATTGTTGT	135.86615
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29104_Cjap_Sim2018_COI	PLO-B30	1	BD29104_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29104_Esin_And2018_Cyb	PLO-B30	1	BD29104_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B30	Cytb	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	ACCCCTCCTCATATCCAACCA	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29104_Mgig_Bot2012_COI	PLO-B30	1	BD29104_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	TCTTATTCGTTGGAGACTTTATAACCCT	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29104_Mjap_Gib2022_COI	PLO-B30	1	BD29104_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	AATTTAATTGTGACTAGGCATGGTCTG	135.86831
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29105_Cmae_Bot2011_COI	PLO-B29	1	BD29105_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	ATGAACAGTCTATCCTCCTTTAG	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29105_Hsan_Wil2021_COI	PLO-B29	1	BD29105_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	CCTGGGCCGGTATAGTAGGT	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29105_Mros_Gib2022_COI	PLO-B29	1	BD29105_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29105_Msal_Bot2012_COI	PLO-B29	1	BD29105_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	GYTAGTTCCRATGATGTTAGCTG	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29105_Mstr_Wil2021_COI	PLO-B29	1	BD29105_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	GTAGTGGTAACTACTCATGCTTTA	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29105_Pvir_Dia2013_COI	PLO-B29	1	BD29105_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	CTTAGTGGCATTAATTCGDAATCC	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29105_Rhar_Sim2018_COI	PLO-B29	1	BD29105_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	CTGCTGCTATTGCTCATG	135.86743
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29107_Aamu_Bax2006_COI	PLO-B27	1	BD29107_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	GCACAACCGGGATCTTTACTTC	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29107_Pcan_Bot2011_IGS	PLO-B27	1	BD29107_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B27	IGS	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	CGTAATCCTCAGTACTGGCTA	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29107_Pper_Dia2013_COI	PLO-B27	1	BD29107_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	CTTAGTGGCATTAATTCGDAATCC	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29107_Sspa_Oph2007_COI	PLO-B27	1	BD29107_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	GAAGGCCTTCCGATGATC	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29107_Upin_Bot2011_COI	PLO-B27	1	BD29107_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	CTTTAATTACAGCGTTTTTATTGTTGT	135.86401
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29108_Cjap_Sim2018_COI	PLO-B26	1	BD29108_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29108_Esin_And2018_Cyb	PLO-B26	1	BD29108_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B26	Cytb	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	ACCCCTCCTCATATCCAACCA	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29108_Mgig_Bot2012_COI	PLO-B26	1	BD29108_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	TCTTATTCGTTGGAGACTTTATAACCCT	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29108_Mjap_Gib2022_COI	PLO-B26	1	BD29108_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	AATTTAATTGTGACTAGGCATGGTCTG	135.86628
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29109_Cmae_Bot2011_COI	PLO-B25	1	BD29109_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	ATGAACAGTCTATCCTCCTTTAG	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29109_Hsan_Wil2021_COI	PLO-B25	1	BD29109_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	CCTGGGCCGGTATAGTAGGT	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29109_Mros_Gib2022_COI	PLO-B25	1	BD29109_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29109_Msal_Bot2012_COI	PLO-B25	1	BD29109_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	GYTAGTTCCRATGATGTTAGCTG	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29109_Mstr_Wil2021_COI	PLO-B25	1	BD29109_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	GTAGTGGTAACTACTCATGCTTTA	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29109_Pvir_Dia2013_COI	PLO-B25	1	BD29109_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	CTTAGTGGCATTAATTCGDAATCC	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29109_Rhar_Sim2018_COI	PLO-B25	1	BD29109_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	CTGCTGCTATTGCTCATG	135.86842
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29110_Aamu_Bax2006_COI	PLO-B24	1	BD29110_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	GCACAACCGGGATCTTTACTTC	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29110_Pcan_Bot2011_IGS	PLO-B24	1	BD29110_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B24	IGS	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	CGTAATCCTCAGTACTGGCTA	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29110_Pper_Dia2013_COI	PLO-B24	1	BD29110_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	CTTAGTGGCATTAATTCGDAATCC	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29110_Sspa_Oph2007_COI	PLO-B24	1	BD29110_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	GAAGGCCTTCCGATGATC	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29110_Upin_Bot2011_COI	PLO-B24	1	BD29110_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	CTTTAATTACAGCGTTTTTATTGTTGT	135.86741
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29111_Cjap_Sim2018_COI	PLO-B23	1	BD29111_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29111_Esin_And2018_Cyb	PLO-B23	1	BD29111_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B23	Cytb	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	ACCCCTCCTCATATCCAACCA	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29111_Mgig_Bot2012_COI	PLO-B23	1	BD29111_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	TCTTATTCGTTGGAGACTTTATAACCCT	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29111_Mjap_Gib2022_COI	PLO-B23	1	BD29111_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	AATTTAATTGTGACTAGGCATGGTCTG	135.86511
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29112_Cmae_Bot2011_COI	PLO-B22	1	BD29112_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	ATGAACAGTCTATCCTCCTTTAG	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29112_Hsan_Wil2021_COI	PLO-B22	1	BD29112_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	CCTGGGCCGGTATAGTAGGT	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29112_Mros_Gib2022_COI	PLO-B22	1	BD29112_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29112_Msal_Bot2012_COI	PLO-B22	1	BD29112_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	GYTAGTTCCRATGATGTTAGCTG	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29112_Mstr_Wil2021_COI	PLO-B22	1	BD29112_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	GTAGTGGTAACTACTCATGCTTTA	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29112_Pvir_Dia2013_COI	PLO-B22	1	BD29112_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	CTTAGTGGCATTAATTCGDAATCC	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29112_Rhar_Sim2018_COI	PLO-B22	1	BD29112_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	CTGCTGCTATTGCTCATG	135.86435
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29114_Aamu_Bax2006_COI	PLO-B20	1	BD29114_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	GCACAACCGGGATCTTTACTTC	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29114_Pcan_Bot2011_IGS	PLO-B20	1	BD29114_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B20	IGS	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	CGTAATCCTCAGTACTGGCTA	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29114_Pper_Dia2013_COI	PLO-B20	1	BD29114_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	CTTAGTGGCATTAATTCGDAATCC	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29114_Sspa_Oph2007_COI	PLO-B20	1	BD29114_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	GAAGGCCTTCCGATGATC	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29114_Upin_Bot2011_COI	PLO-B20	1	BD29114_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	CTTTAATTACAGCGTTTTTATTGTTGT	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71153	WGS84	BD29114	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29114_Vgib_Wil2025_COI	PLO-B20	1	BD29114_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	CCCCTCTTTACAAGTTCTTTAGGGT	135.8694
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71152	WGS84	BD29115	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29115_Asen_Wil2025_COI	PLO-B19	1	BD29115_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	GTTGTTGTTACAACACATGCTCTTTTG	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29115_Cjap_Sim2018_COI	PLO-B19	1	BD29115_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29115_Esin_And2018_Cyb	PLO-B19	1	BD29115_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B19	Cytb	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	ACCCCTCCTCATATCCAACCA	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29115_Mgig_Bot2012_COI	PLO-B19	1	BD29115_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	TCTTATTCGTTGGAGACTTTATAACCCT	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29115_Mjap_Gib2022_COI	PLO-B19	1	BD29115_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	AATTTAATTGTGACTAGGCATGGTCTG	135.87177
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29116_Cmae_Bot2011_COI	PLO-B18	1	BD29116_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	ATGAACAGTCTATCCTCCTTTAG	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29116_Hsan_Wil2021_COI	PLO-B18	1	BD29116_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	CCTGGGCCGGTATAGTAGGT	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29116_Mros_Gib2022_COI	PLO-B18	1	BD29116_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29116_Msal_Bot2012_COI	PLO-B18	1	BD29116_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	GYTAGTTCCRATGATGTTAGCTG	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29116_Mstr_Wil2021_COI	PLO-B18	1	BD29116_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	GTAGTGGTAACTACTCATGCTTTA	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29116_Pvir_Dia2013_COI	PLO-B18	1	BD29116_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	CTTAGTGGCATTAATTCGDAATCC	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29116_Rhar_Sim2018_COI	PLO-B18	1	BD29116_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	CTGCTGCTATTGCTCATG	135.87388
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29118_Aamu_Bax2006_COI	PLO-B16	1	BD29118_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	GCACAACCGGGATCTTTACTTC	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29118_Pcan_Bot2011_IGS	PLO-B16	1	BD29118_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B16	IGS	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	CGTAATCCTCAGTACTGGCTA	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29118_Pper_Dia2013_COI	PLO-B16	1	BD29118_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	CTTAGTGGCATTAATTCGDAATCC	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29118_Sspa_Oph2007_COI	PLO-B16	1	BD29118_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	GAAGGCCTTCCGATGATC	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29118_Upin_Bot2011_COI	PLO-B16	1	BD29118_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	CTTTAATTACAGCGTTTTTATTGTTGT	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71312	WGS84	BD29118	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29118_Vgib_Wil2025_COI	PLO-B16	1	BD29118_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	CCCCTCTTTACAAGTTCTTTAGGGT	135.87288
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71468	WGS84	BD29119	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29119_Asen_Wil2025_COI	PLO-B15	1	BD29119_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	GTTGTTGTTACAACACATGCTCTTTTG	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29119_Cjap_Sim2018_COI	PLO-B15	1	BD29119_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29119_Esin_And2018_Cyb	PLO-B15	1	BD29119_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B15	Cytb	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	ACCCCTCCTCATATCCAACCA	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29119_Mgig_Bot2012_COI	PLO-B15	1	BD29119_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	TCTTATTCGTTGGAGACTTTATAACCCT	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29119_Mjap_Gib2022_COI	PLO-B15	1	BD29119_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	AATTTAATTGTGACTAGGCATGGTCTG	135.87184
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29121_Aamu_Bax2006_COI	PLO-B13	1	BD29121_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	GCACAACCGGGATCTTTACTTC	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29121_Pcan_Bot2011_IGS	PLO-B13	1	BD29121_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B13	IGS	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	CGTAATCCTCAGTACTGGCTA	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29121_Pper_Dia2013_COI	PLO-B13	1	BD29121_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	CTTAGTGGCATTAATTCGDAATCC	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29121_Sspa_Oph2007_COI	PLO-B13	1	BD29121_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	GAAGGCCTTCCGATGATC	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29121_Upin_Bot2011_COI	PLO-B13	1	BD29121_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	CTTTAATTACAGCGTTTTTATTGTTGT	135.87618
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29122_Cjap_Sim2018_COI	PLO-B12	1	BD29122_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29122_Esin_And2018_Cyb	PLO-B12	1	BD29122_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B12	Cytb	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	ACCCCTCCTCATATCCAACCA	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29122_Mgig_Bot2012_COI	PLO-B12	1	BD29122_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	TCTTATTCGTTGGAGACTTTATAACCCT	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29122_Mjap_Gib2022_COI	PLO-B12	1	BD29122_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	AATTTAATTGTGACTAGGCATGGTCTG	135.87842
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29123_Cmae_Bot2011_COI	PLO-B11	1	BD29123_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	ATGAACAGTCTATCCTCCTTTAG	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29123_Hsan_Wil2021_COI	PLO-B11	1	BD29123_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	CCTGGGCCGGTATAGTAGGT	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29123_Mros_Gib2022_COI	PLO-B11	1	BD29123_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29123_Msal_Bot2012_COI	PLO-B11	1	BD29123_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	GYTAGTTCCRATGATGTTAGCTG	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29123_Mstr_Wil2021_COI	PLO-B11	1	BD29123_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	GTAGTGGTAACTACTCATGCTTTA	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29123_Pvir_Dia2013_COI	PLO-B11	1	BD29123_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	CTTAGTGGCATTAATTCGDAATCC	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29123_Rhar_Sim2018_COI	PLO-B11	1	BD29123_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	CTGCTGCTATTGCTCATG	135.87959
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29125_Aamu_Bax2006_COI	PLO-B09	1	BD29125_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	GCACAACCGGGATCTTTACTTC	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29125_Pcan_Bot2011_IGS	PLO-B09	1	BD29125_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B09	IGS	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	CGTAATCCTCAGTACTGGCTA	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29125_Pper_Dia2013_COI	PLO-B09	1	BD29125_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	CTTAGTGGCATTAATTCGDAATCC	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29125_Sspa_Oph2007_COI	PLO-B09	1	BD29125_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	GAAGGCCTTCCGATGATC	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29125_Upin_Bot2011_COI	PLO-B09	1	BD29125_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	CTTTAATTACAGCGTTTTTATTGTTGT	135.87514
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29126_Cjap_Sim2018_COI	PLO-B08	1	BD29126_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29126_Esin_And2018_Cyb	PLO-B08	1	BD29126_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B08	Cytb	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	ACCCCTCCTCATATCCAACCA	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29126_Mgig_Bot2012_COI	PLO-B08	1	BD29126_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	TCTTATTCGTTGGAGACTTTATAACCCT	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29126_Mjap_Gib2022_COI	PLO-B08	1	BD29126_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	AATTTAATTGTGACTAGGCATGGTCTG	135.87292
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29127_Cmae_Bot2011_COI	PLO-B07	1	BD29127_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	ATGAACAGTCTATCCTCCTTTAG	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29127_Hsan_Wil2021_COI	PLO-B07	1	BD29127_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	CCTGGGCCGGTATAGTAGGT	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29127_Mros_Gib2022_COI	PLO-B07	1	BD29127_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29127_Msal_Bot2012_COI	PLO-B07	1	BD29127_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	GYTAGTTCCRATGATGTTAGCTG	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29127_Mstr_Wil2021_COI	PLO-B07	1	BD29127_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	GTAGTGGTAACTACTCATGCTTTA	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29127_Pvir_Dia2013_COI	PLO-B07	1	BD29127_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	CTTAGTGGCATTAATTCGDAATCC	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29127_Rhar_Sim2018_COI	PLO-B07	1	BD29127_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	CTGCTGCTATTGCTCATG	135.8712
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29129_Aamu_Bax2006_COI	PLO-B05	1	BD29129_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	GCACAACCGGGATCTTTACTTC	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29129_Pcan_Bot2011_IGS	PLO-B05	1	BD29129_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B05	IGS	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	CGTAATCCTCAGTACTGGCTA	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29129_Pper_Dia2013_COI	PLO-B05	1	BD29129_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	CTTAGTGGCATTAATTCGDAATCC	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29129_Sspa_Oph2007_COI	PLO-B05	1	BD29129_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	GAAGGCCTTCCGATGATC	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29129_Upin_Bot2011_COI	PLO-B05	1	BD29129_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	CTTTAATTACAGCGTTTTTATTGTTGT	135.87293
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29130_Cmae_Bot2011_COI	PLO-B04	1	BD29130_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	ATGAACAGTCTATCCTCCTTTAG	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29130_Hsan_Wil2021_COI	PLO-B04	1	BD29130_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	CCTGGGCCGGTATAGTAGGT	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29130_Mros_Gib2022_COI	PLO-B04	1	BD29130_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29130_Msal_Bot2012_COI	PLO-B04	1	BD29130_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	GYTAGTTCCRATGATGTTAGCTG	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29130_Mstr_Wil2021_COI	PLO-B04	1	BD29130_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	GTAGTGGTAACTACTCATGCTTTA	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29130_Pvir_Dia2013_COI	PLO-B04	1	BD29130_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	CTTAGTGGCATTAATTCGDAATCC	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29130_Rhar_Sim2018_COI	PLO-B04	1	BD29130_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	CTGCTGCTATTGCTCATG	135.87408
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29132_Aamu_Bax2006_COI	PLO-B02	1	BD29132_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	GCACAACCGGGATCTTTACTTC	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29132_Pcan_Bot2011_IGS	PLO-B02	1	BD29132_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B02	IGS	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	CGTAATCCTCAGTACTGGCTA	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29132_Pper_Dia2013_COI	PLO-B02	1	BD29132_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	CTTAGTGGCATTAATTCGDAATCC	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29132_Sspa_Oph2007_COI	PLO-B02	1	BD29132_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	GAAGGCCTTCCGATGATC	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29132_Upin_Bot2011_COI	PLO-B02	1	BD29132_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	CTTTAATTACAGCGTTTTTATTGTTGT	135.87846
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29133_Cjap_Sim2018_COI	PLO-B01	1	BD29133_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29133_Esin_And2018_Cyb	PLO-B01	1	BD29133_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B01	Cytb	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	ACCCCTCCTCATATCCAACCA	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29133_Mgig_Bot2012_COI	PLO-B01	1	BD29133_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	TCTTATTCGTTGGAGACTTTATAACCCT	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29133_Mjap_Gib2022_COI	PLO-B01	1	BD29133_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	AATTTAATTGTGACTAGGCATGGTCTG	135.88088
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.717	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29134_Cmae_Bot2011_COI	ADL-A70	1	BD29134_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	ATGAACAGTCTATCCTCCTTTAG	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29134_Hsan_Wil2021_COI	ADL-A70	1	BD29134_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	CCTGGGCCGGTATAGTAGGT	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29134_Mros_Gib2022_COI	ADL-A70	1	BD29134_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29134_Msal_Bot2012_COI	ADL-A70	1	BD29134_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	GYTAGTTCCRATGATGTTAGCTG	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29134_Mstr_Wil2021_COI	ADL-A70	1	BD29134_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	GTAGTGGTAACTACTCATGCTTTA	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29134_Pvir_Dia2013_COI	ADL-A70	1	BD29134_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	CTTAGTGGCATTAATTCGDAATCC	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29134_Rhar_Sim2018_COI	ADL-A70	1	BD29134_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	CTGCTGCTATTGCTCATG	138.51415
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29136_Aamu_Bax2006_COI	ADL-A68	1	BD29136_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	GCACAACCGGGATCTTTACTTC	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29136_Pcan_Bot2011_IGS	ADL-A68	1	BD29136_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A68	IGS	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	CGTAATCCTCAGTACTGGCTA	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29136_Pper_Dia2013_COI	ADL-A68	1	BD29136_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	CTTAGTGGCATTAATTCGDAATCC	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.19	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29136_Sspa_Oph2007_COI	ADL-A68	1	BD29136_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	GAAGGCCTTCCGATGATC	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29136_Upin_Bot2011_COI	ADL-A68	1	BD29136_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	CTTTAATTACAGCGTTTTTATTGTTGT	138.51366
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29137_Cjap_Sim2018_COI	ADL-A67	1	BD29137_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29137_Esin_And2018_Cyb	ADL-A67	1	BD29137_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A67	Cytb	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	ACCCCTCCTCATATCCAACCA	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29137_Mgig_Bot2012_COI	ADL-A67	1	BD29137_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	TCTTATTCGTTGGAGACTTTATAACCCT	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29137_Mjap_Gib2022_COI	ADL-A67	1	BD29137_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	AATTTAATTGTGACTAGGCATGGTCTG	138.51305
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	26.525	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29138_Cmae_Bot2011_COI	ADL-A66	1	BD29138_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	ATGAACAGTCTATCCTCCTTTAG	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29138_Hsan_Wil2021_COI	ADL-A66	1	BD29138_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	CCTGGGCCGGTATAGTAGGT	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29138_Mros_Gib2022_COI	ADL-A66	1	BD29138_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29138_Msal_Bot2012_COI	ADL-A66	1	BD29138_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	GYTAGTTCCRATGATGTTAGCTG	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29138_Mstr_Wil2021_COI	ADL-A66	1	BD29138_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	GTAGTGGTAACTACTCATGCTTTA	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29138_Pvir_Dia2013_COI	ADL-A66	1	BD29138_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	CTTAGTGGCATTAATTCGDAATCC	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29138_Rhar_Sim2018_COI	ADL-A66	1	BD29138_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	CTGCTGCTATTGCTCATG	138.51382
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29140_Cjap_Sim2018_COI	ADL-A64	1	BD29140_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29140_Esin_And2018_Cyb	ADL-A64	1	BD29140_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A64	Cytb	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	ACCCCTCCTCATATCCAACCA	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29140_Mgig_Bot2012_COI	ADL-A64	1	BD29140_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	TCTTATTCGTTGGAGACTTTATAACCCT	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29140_Mjap_Gib2022_COI	ADL-A64	1	BD29140_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	AATTTAATTGTGACTAGGCATGGTCTG	138.51268
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	26.563	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29141_Cmae_Bot2011_COI	ADL-A63	1	BD29141_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	ATGAACAGTCTATCCTCCTTTAG	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29141_Hsan_Wil2021_COI	ADL-A63	1	BD29141_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	CCTGGGCCGGTATAGTAGGT	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29141_Mros_Gib2022_COI	ADL-A63	1	BD29141_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29141_Msal_Bot2012_COI	ADL-A63	1	BD29141_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	GYTAGTTCCRATGATGTTAGCTG	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29141_Mstr_Wil2021_COI	ADL-A63	1	BD29141_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	GTAGTGGTAACTACTCATGCTTTA	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29141_Pvir_Dia2013_COI	ADL-A63	1	BD29141_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	CTTAGTGGCATTAATTCGDAATCC	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29141_Rhar_Sim2018_COI	ADL-A63	1	BD29141_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	CTGCTGCTATTGCTCATG	138.51165
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29143_Aamu_Bax2006_COI	ADL-A61	1	BD29143_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	GCACAACCGGGATCTTTACTTC	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29143_Pcan_Bot2011_IGS	ADL-A61	1	BD29143_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A61	IGS	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	CGTAATCCTCAGTACTGGCTA	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29143_Pper_Dia2013_COI	ADL-A61	1	BD29143_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	CTTAGTGGCATTAATTCGDAATCC	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.089	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29143_Sspa_Oph2007_COI	ADL-A61	1	BD29143_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	GAAGGCCTTCCGATGATC	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29143_Upin_Bot2011_COI	ADL-A61	1	BD29143_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	CTTTAATTACAGCGTTTTTATTGTTGT	138.51199
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29144_Cjap_Sim2018_COI	ADL-A60	1	BD29144_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29144_Esin_And2018_Cyb	ADL-A60	1	BD29144_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A60	Cytb	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	ACCCCTCCTCATATCCAACCA	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29144_Mgig_Bot2012_COI	ADL-A60	1	BD29144_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	TCTTATTCGTTGGAGACTTTATAACCCT	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29144_Mjap_Gib2022_COI	ADL-A60	1	BD29144_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	AATTTAATTGTGACTAGGCATGGTCTG	138.5106
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.822	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29145_Cmae_Bot2011_COI	ADL-A59	1	BD29145_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	ATGAACAGTCTATCCTCCTTTAG	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29145_Hsan_Wil2021_COI	ADL-A59	1	BD29145_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	CCTGGGCCGGTATAGTAGGT	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29145_Mros_Gib2022_COI	ADL-A59	1	BD29145_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29145_Msal_Bot2012_COI	ADL-A59	1	BD29145_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	GYTAGTTCCRATGATGTTAGCTG	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29145_Mstr_Wil2021_COI	ADL-A59	1	BD29145_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	GTAGTGGTAACTACTCATGCTTTA	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29145_Pvir_Dia2013_COI	ADL-A59	1	BD29145_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	CTTAGTGGCATTAATTCGDAATCC	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29145_Rhar_Sim2018_COI	ADL-A59	1	BD29145_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	CTGCTGCTATTGCTCATG	138.51145
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29147_Aamu_Bax2006_COI	ADL-A57	1	BD29147_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	GCACAACCGGGATCTTTACTTC	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29147_Pcan_Bot2011_IGS	ADL-A57	1	BD29147_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A57	IGS	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	CGTAATCCTCAGTACTGGCTA	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29147_Pper_Dia2013_COI	ADL-A57	1	BD29147_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	CTTAGTGGCATTAATTCGDAATCC	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.384	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29147_Sspa_Oph2007_COI	ADL-A57	1	BD29147_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	GAAGGCCTTCCGATGATC	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29147_Upin_Bot2011_COI	ADL-A57	1	BD29147_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	CTTTAATTACAGCGTTTTTATTGTTGT	138.51067
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29148_Cjap_Sim2018_COI	ADL-A56	1	BD29148_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29148_Esin_And2018_Cyb	ADL-A56	1	BD29148_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A56	Cytb	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	ACCCCTCCTCATATCCAACCA	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29148_Mgig_Bot2012_COI	ADL-A56	1	BD29148_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	TCTTATTCGTTGGAGACTTTATAACCCT	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29148_Mjap_Gib2022_COI	ADL-A56	1	BD29148_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	AATTTAATTGTGACTAGGCATGGTCTG	138.50936
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.72	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29149_Cmae_Bot2011_COI	ADL-A55	1	BD29149_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	ATGAACAGTCTATCCTCCTTTAG	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29149_Hsan_Wil2021_COI	ADL-A55	1	BD29149_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	CCTGGGCCGGTATAGTAGGT	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29149_Mros_Gib2022_COI	ADL-A55	1	BD29149_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29149_Msal_Bot2012_COI	ADL-A55	1	BD29149_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	GYTAGTTCCRATGATGTTAGCTG	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29149_Mstr_Wil2021_COI	ADL-A55	1	BD29149_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	GTAGTGGTAACTACTCATGCTTTA	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29149_Pvir_Dia2013_COI	ADL-A55	1	BD29149_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	CTTAGTGGCATTAATTCGDAATCC	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29149_Rhar_Sim2018_COI	ADL-A55	1	BD29149_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	CTGCTGCTATTGCTCATG	138.51001
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29150_Aamu_Bax2006_COI	ADL-A54	1	BD29150_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	GCACAACCGGGATCTTTACTTC	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29150_Pcan_Bot2011_IGS	ADL-A54	1	BD29150_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A54	IGS	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	CGTAATCCTCAGTACTGGCTA	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29150_Pper_Dia2013_COI	ADL-A54	1	BD29150_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	CTTAGTGGCATTAATTCGDAATCC	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.007	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29150_Sspa_Oph2007_COI	ADL-A54	1	BD29150_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	GAAGGCCTTCCGATGATC	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29150_Upin_Bot2011_COI	ADL-A54	1	BD29150_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	CTTTAATTACAGCGTTTTTATTGTTGT	138.50873
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29151_Cjap_Sim2018_COI	ADL-A53	1	BD29151_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29151_Esin_And2018_Cyb	ADL-A53	1	BD29151_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A53	Cytb	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	ACCCCTCCTCATATCCAACCA	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29151_Mgig_Bot2012_COI	ADL-A53	1	BD29151_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	TCTTATTCGTTGGAGACTTTATAACCCT	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29151_Mjap_Gib2022_COI	ADL-A53	1	BD29151_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	AATTTAATTGTGACTAGGCATGGTCTG	138.5094
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29152_Cmae_Bot2011_COI	ADL-A52	1	BD29152_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	ATGAACAGTCTATCCTCCTTTAG	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29152_Hsan_Wil2021_COI	ADL-A52	1	BD29152_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	CCTGGGCCGGTATAGTAGGT	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29152_Mros_Gib2022_COI	ADL-A52	1	BD29152_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29152_Msal_Bot2012_COI	ADL-A52	1	BD29152_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	GYTAGTTCCRATGATGTTAGCTG	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29152_Mstr_Wil2021_COI	ADL-A52	1	BD29152_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	GTAGTGGTAACTACTCATGCTTTA	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29152_Pvir_Dia2013_COI	ADL-A52	1	BD29152_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	CTTAGTGGCATTAATTCGDAATCC	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29152_Rhar_Sim2018_COI	ADL-A52	1	BD29152_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	CTGCTGCTATTGCTCATG	138.50802
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29154_Aamu_Bax2006_COI	ADL-A50	1	BD29154_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	GCACAACCGGGATCTTTACTTC	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29154_Pcan_Bot2011_IGS	ADL-A50	1	BD29154_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A50	IGS	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	CGTAATCCTCAGTACTGGCTA	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29154_Pper_Dia2013_COI	ADL-A50	1	BD29154_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	CTTAGTGGCATTAATTCGDAATCC	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.89	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29154_Sspa_Oph2007_COI	ADL-A50	1	BD29154_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	GAAGGCCTTCCGATGATC	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29154_Upin_Bot2011_COI	ADL-A50	1	BD29154_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	CTTTAATTACAGCGTTTTTATTGTTGT	138.5073
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29155_Cjap_Sim2018_COI	ADL-A49	1	BD29155_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29155_Esin_And2018_Cyb	ADL-A49	1	BD29155_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A49	Cytb	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	ACCCCTCCTCATATCCAACCA	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29155_Mgig_Bot2012_COI	ADL-A49	1	BD29155_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	TCTTATTCGTTGGAGACTTTATAACCCT	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29155_Mjap_Gib2022_COI	ADL-A49	1	BD29155_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	AATTTAATTGTGACTAGGCATGGTCTG	138.50794
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29156_Cmae_Bot2011_COI	ADL-A48	1	BD29156_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	ATGAACAGTCTATCCTCCTTTAG	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29156_Hsan_Wil2021_COI	ADL-A48	1	BD29156_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	CCTGGGCCGGTATAGTAGGT	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29156_Mros_Gib2022_COI	ADL-A48	1	BD29156_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29156_Msal_Bot2012_COI	ADL-A48	1	BD29156_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	GYTAGTTCCRATGATGTTAGCTG	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29156_Mstr_Wil2021_COI	ADL-A48	1	BD29156_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	GTAGTGGTAACTACTCATGCTTTA	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29156_Pvir_Dia2013_COI	ADL-A48	1	BD29156_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	CTTAGTGGCATTAATTCGDAATCC	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29156_Rhar_Sim2018_COI	ADL-A48	1	BD29156_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	CTGCTGCTATTGCTCATG	138.50888
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29158_Aamu_Bax2006_COI	ADL-A46	1	BD29158_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	GCACAACCGGGATCTTTACTTC	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29158_Pcan_Bot2011_IGS	ADL-A46	1	BD29158_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A46	IGS	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	CGTAATCCTCAGTACTGGCTA	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29158_Pper_Dia2013_COI	ADL-A46	1	BD29158_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	CTTAGTGGCATTAATTCGDAATCC	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.451	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29158_Sspa_Oph2007_COI	ADL-A46	1	BD29158_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	GAAGGCCTTCCGATGATC	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29158_Upin_Bot2011_COI	ADL-A46	1	BD29158_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	CTTTAATTACAGCGTTTTTATTGTTGT	138.50951
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29159_Cjap_Sim2018_COI	ADL-A45	1	BD29159_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29159_Esin_And2018_Cyb	ADL-A45	1	BD29159_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A45	Cytb	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	ACCCCTCCTCATATCCAACCA	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29159_Mgig_Bot2012_COI	ADL-A45	1	BD29159_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	TCTTATTCGTTGGAGACTTTATAACCCT	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29159_Mjap_Gib2022_COI	ADL-A45	1	BD29159_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	AATTTAATTGTGACTAGGCATGGTCTG	138.50813
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29161_Aamu_Bax2006_COI	ADL-A43	1	BD29161_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	GCACAACCGGGATCTTTACTTC	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29161_Pcan_Bot2011_IGS	ADL-A43	1	BD29161_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A43	IGS	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	CGTAATCCTCAGTACTGGCTA	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29161_Pper_Dia2013_COI	ADL-A43	1	BD29161_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	CTTAGTGGCATTAATTCGDAATCC	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.532	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29161_Sspa_Oph2007_COI	ADL-A43	1	BD29161_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	GAAGGCCTTCCGATGATC	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29161_Upin_Bot2011_COI	ADL-A43	1	BD29161_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	CTTTAATTACAGCGTTTTTATTGTTGT	138.50739
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29162_Cjap_Sim2018_COI	ADL-A42	1	BD29162_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29162_Esin_And2018_Cyb	ADL-A42	1	BD29162_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A42	Cytb	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	ACCCCTCCTCATATCCAACCA	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29162_Mgig_Bot2012_COI	ADL-A42	1	BD29162_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	TCTTATTCGTTGGAGACTTTATAACCCT	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29162_Mjap_Gib2022_COI	ADL-A42	1	BD29162_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	AATTTAATTGTGACTAGGCATGGTCTG	138.50687
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.457	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29163_Cmae_Bot2011_COI	ADL-A41	1	BD29163_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	ATGAACAGTCTATCCTCCTTTAG	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29163_Hsan_Wil2021_COI	ADL-A41	1	BD29163_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	CCTGGGCCGGTATAGTAGGT	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29163_Mros_Gib2022_COI	ADL-A41	1	BD29163_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29163_Msal_Bot2012_COI	ADL-A41	1	BD29163_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	GYTAGTTCCRATGATGTTAGCTG	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29163_Mstr_Wil2021_COI	ADL-A41	1	BD29163_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	GTAGTGGTAACTACTCATGCTTTA	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29163_Pvir_Dia2013_COI	ADL-A41	1	BD29163_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	CTTAGTGGCATTAATTCGDAATCC	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29163_Rhar_Sim2018_COI	ADL-A41	1	BD29163_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	CTGCTGCTATTGCTCATG	138.50654
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29165_Aamu_Bax2006_COI	ADL-A39	1	BD29165_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	GCACAACCGGGATCTTTACTTC	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29165_Pcan_Bot2011_IGS	ADL-A39	1	BD29165_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A39	IGS	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	CGTAATCCTCAGTACTGGCTA	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29165_Pper_Dia2013_COI	ADL-A39	1	BD29165_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	CTTAGTGGCATTAATTCGDAATCC	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.951	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29165_Sspa_Oph2007_COI	ADL-A39	1	BD29165_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	GAAGGCCTTCCGATGATC	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29165_Upin_Bot2011_COI	ADL-A39	1	BD29165_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	CTTTAATTACAGCGTTTTTATTGTTGT	138.50579
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29166_Cjap_Sim2018_COI	ADL-A38	1	BD29166_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29166_Esin_And2018_Cyb	ADL-A38	1	BD29166_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A38	Cytb	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	ACCCCTCCTCATATCCAACCA	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29166_Mgig_Bot2012_COI	ADL-A38	1	BD29166_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	TCTTATTCGTTGGAGACTTTATAACCCT	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29166_Mjap_Gib2022_COI	ADL-A38	1	BD29166_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	AATTTAATTGTGACTAGGCATGGTCTG	138.50531
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29167_Cmae_Bot2011_COI	ADL-A37	1	BD29167_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	ATGAACAGTCTATCCTCCTTTAG	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29167_Hsan_Wil2021_COI	ADL-A37	1	BD29167_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	CCTGGGCCGGTATAGTAGGT	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29167_Mros_Gib2022_COI	ADL-A37	1	BD29167_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29167_Msal_Bot2012_COI	ADL-A37	1	BD29167_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	GYTAGTTCCRATGATGTTAGCTG	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29167_Mstr_Wil2021_COI	ADL-A37	1	BD29167_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	GTAGTGGTAACTACTCATGCTTTA	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29167_Pvir_Dia2013_COI	ADL-A37	1	BD29167_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	CTTAGTGGCATTAATTCGDAATCC	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29167_Rhar_Sim2018_COI	ADL-A37	1	BD29167_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	CTGCTGCTATTGCTCATG	138.50054
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29169_Aamu_Bax2006_COI	ADL-A35	1	BD29169_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	GCACAACCGGGATCTTTACTTC	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29169_Pcan_Bot2011_IGS	ADL-A35	1	BD29169_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A35	IGS	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	CGTAATCCTCAGTACTGGCTA	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29169_Pper_Dia2013_COI	ADL-A35	1	BD29169_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	CTTAGTGGCATTAATTCGDAATCC	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.382	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29169_Sspa_Oph2007_COI	ADL-A35	1	BD29169_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	GAAGGCCTTCCGATGATC	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29169_Upin_Bot2011_COI	ADL-A35	1	BD29169_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	CTTTAATTACAGCGTTTTTATTGTTGT	138.5
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.513	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29170_Cmae_Bot2011_COI	ADL-A34	1	BD29170_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	ATGAACAGTCTATCCTCCTTTAG	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29170_Hsan_Wil2021_COI	ADL-A34	1	BD29170_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	CCTGGGCCGGTATAGTAGGT	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29170_Mros_Gib2022_COI	ADL-A34	1	BD29170_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29170_Msal_Bot2012_COI	ADL-A34	1	BD29170_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	GYTAGTTCCRATGATGTTAGCTG	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29170_Mstr_Wil2021_COI	ADL-A34	1	BD29170_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	GTAGTGGTAACTACTCATGCTTTA	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29170_Pvir_Dia2013_COI	ADL-A34	1	BD29170_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	CTTAGTGGCATTAATTCGDAATCC	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29170_Rhar_Sim2018_COI	ADL-A34	1	BD29170_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	CTGCTGCTATTGCTCATG	138.49805
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29172_Aamu_Bax2006_COI	ADL-A32	1	BD29172_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	GCACAACCGGGATCTTTACTTC	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29172_Pcan_Bot2011_IGS	ADL-A32	1	BD29172_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A32	IGS	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	CGTAATCCTCAGTACTGGCTA	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29172_Pper_Dia2013_COI	ADL-A32	1	BD29172_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	CTTAGTGGCATTAATTCGDAATCC	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.506	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29172_Sspa_Oph2007_COI	ADL-A32	1	BD29172_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	GAAGGCCTTCCGATGATC	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29172_Upin_Bot2011_COI	ADL-A32	1	BD29172_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	CTTTAATTACAGCGTTTTTATTGTTGT	138.49913
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29173_Cjap_Sim2018_COI	ADL-A31	1	BD29173_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29173_Esin_And2018_Cyb	ADL-A31	1	BD29173_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A31	Cytb	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	ACCCCTCCTCATATCCAACCA	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29173_Mgig_Bot2012_COI	ADL-A31	1	BD29173_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	TCTTATTCGTTGGAGACTTTATAACCCT	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29173_Mjap_Gib2022_COI	ADL-A31	1	BD29173_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	AATTTAATTGTGACTAGGCATGGTCTG	138.49696
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.303	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29174_Cmae_Bot2011_COI	ADL-A30	1	BD29174_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	ATGAACAGTCTATCCTCCTTTAG	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29174_Hsan_Wil2021_COI	ADL-A30	1	BD29174_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	CCTGGGCCGGTATAGTAGGT	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29174_Mros_Gib2022_COI	ADL-A30	1	BD29174_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29174_Msal_Bot2012_COI	ADL-A30	1	BD29174_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	GYTAGTTCCRATGATGTTAGCTG	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29174_Mstr_Wil2021_COI	ADL-A30	1	BD29174_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	GTAGTGGTAACTACTCATGCTTTA	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29174_Pvir_Dia2013_COI	ADL-A30	1	BD29174_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	CTTAGTGGCATTAATTCGDAATCC	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29174_Rhar_Sim2018_COI	ADL-A30	1	BD29174_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	CTGCTGCTATTGCTCATG	138.49501
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29176_Aamu_Bax2006_COI	ADL-A28	1	BD29176_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	GCACAACCGGGATCTTTACTTC	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29176_Pcan_Bot2011_IGS	ADL-A28	1	BD29176_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A28	IGS	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	CGTAATCCTCAGTACTGGCTA	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29176_Pper_Dia2013_COI	ADL-A28	1	BD29176_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	CTTAGTGGCATTAATTCGDAATCC	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.122	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29176_Sspa_Oph2007_COI	ADL-A28	1	BD29176_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	GAAGGCCTTCCGATGATC	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29176_Upin_Bot2011_COI	ADL-A28	1	BD29176_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	CTTTAATTACAGCGTTTTTATTGTTGT	138.49389
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29177_Cjap_Sim2018_COI	ADL-A27	1	BD29177_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29177_Esin_And2018_Cyb	ADL-A27	1	BD29177_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A27	Cytb	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	ACCCCTCCTCATATCCAACCA	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29177_Mgig_Bot2012_COI	ADL-A27	1	BD29177_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	TCTTATTCGTTGGAGACTTTATAACCCT	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29177_Mjap_Gib2022_COI	ADL-A27	1	BD29177_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	AATTTAATTGTGACTAGGCATGGTCTG	138.4918
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.37	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29178_Cmae_Bot2011_COI	ADL-A26	1	BD29178_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	ATGAACAGTCTATCCTCCTTTAG	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29178_Hsan_Wil2021_COI	ADL-A26	1	BD29178_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	CCTGGGCCGGTATAGTAGGT	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29178_Mros_Gib2022_COI	ADL-A26	1	BD29178_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29178_Msal_Bot2012_COI	ADL-A26	1	BD29178_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	GYTAGTTCCRATGATGTTAGCTG	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29178_Mstr_Wil2021_COI	ADL-A26	1	BD29178_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	GTAGTGGTAACTACTCATGCTTTA	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29178_Pvir_Dia2013_COI	ADL-A26	1	BD29178_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	CTTAGTGGCATTAATTCGDAATCC	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29178_Rhar_Sim2018_COI	ADL-A26	1	BD29178_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	CTGCTGCTATTGCTCATG	138.49301
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29180_Cjap_Sim2018_COI	ADL-A24	1	BD29180_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29180_Esin_And2018_Cyb	ADL-A24	1	BD29180_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A24	Cytb	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	ACCCCTCCTCATATCCAACCA	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29180_Mgig_Bot2012_COI	ADL-A24	1	BD29180_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	TCTTATTCGTTGGAGACTTTATAACCCT	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29180_Mjap_Gib2022_COI	ADL-A24	1	BD29180_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	AATTTAATTGTGACTAGGCATGGTCTG	138.48685
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.829	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29181_Cmae_Bot2011_COI	ADL-A23	1	BD29181_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	ATGAACAGTCTATCCTCCTTTAG	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29181_Hsan_Wil2021_COI	ADL-A23	1	BD29181_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	CCTGGGCCGGTATAGTAGGT	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29181_Mros_Gib2022_COI	ADL-A23	1	BD29181_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29181_Msal_Bot2012_COI	ADL-A23	1	BD29181_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	GYTAGTTCCRATGATGTTAGCTG	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29181_Mstr_Wil2021_COI	ADL-A23	1	BD29181_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	GTAGTGGTAACTACTCATGCTTTA	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29181_Pvir_Dia2013_COI	ADL-A23	1	BD29181_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	CTTAGTGGCATTAATTCGDAATCC	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29181_Rhar_Sim2018_COI	ADL-A23	1	BD29181_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	CTGCTGCTATTGCTCATG	138.48983
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29183_Aamu_Bax2006_COI	ADL-A21	1	BD29183_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	GCACAACCGGGATCTTTACTTC	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29183_Pcan_Bot2011_IGS	ADL-A21	1	BD29183_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A21	IGS	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	CGTAATCCTCAGTACTGGCTA	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29183_Pper_Dia2013_COI	ADL-A21	1	BD29183_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	CTTAGTGGCATTAATTCGDAATCC	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.444	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29183_Sspa_Oph2007_COI	ADL-A21	1	BD29183_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	GAAGGCCTTCCGATGATC	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29183_Upin_Bot2011_COI	ADL-A21	1	BD29183_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	CTTTAATTACAGCGTTTTTATTGTTGT	138.48607
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29184_Cjap_Sim2018_COI	ADL-A20	1	BD29184_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29184_Esin_And2018_Cyb	ADL-A20	1	BD29184_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A20	Cytb	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	ACCCCTCCTCATATCCAACCA	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29184_Mgig_Bot2012_COI	ADL-A20	1	BD29184_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	TCTTATTCGTTGGAGACTTTATAACCCT	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29184_Mjap_Gib2022_COI	ADL-A20	1	BD29184_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	AATTTAATTGTGACTAGGCATGGTCTG	138.4887
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.272	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29185_Cmae_Bot2011_COI	ADL-A19	1	BD29185_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	ATGAACAGTCTATCCTCCTTTAG	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29185_Hsan_Wil2021_COI	ADL-A19	1	BD29185_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	CCTGGGCCGGTATAGTAGGT	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29185_Mros_Gib2022_COI	ADL-A19	1	BD29185_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29185_Msal_Bot2012_COI	ADL-A19	1	BD29185_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	GYTAGTTCCRATGATGTTAGCTG	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29185_Mstr_Wil2021_COI	ADL-A19	1	BD29185_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	GTAGTGGTAACTACTCATGCTTTA	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29185_Pvir_Dia2013_COI	ADL-A19	1	BD29185_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	CTTAGTGGCATTAATTCGDAATCC	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29185_Rhar_Sim2018_COI	ADL-A19	1	BD29185_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	CTGCTGCTATTGCTCATG	138.48667
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29187_Aamu_Bax2006_COI	ADL-A17	1	BD29187_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	GCACAACCGGGATCTTTACTTC	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29187_Pcan_Bot2011_IGS	ADL-A17	1	BD29187_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A17	IGS	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	CGTAATCCTCAGTACTGGCTA	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29187_Pper_Dia2013_COI	ADL-A17	1	BD29187_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	CTTAGTGGCATTAATTCGDAATCC	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.39	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29187_Sspa_Oph2007_COI	ADL-A17	1	BD29187_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	GAAGGCCTTCCGATGATC	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29187_Upin_Bot2011_COI	ADL-A17	1	BD29187_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	CTTTAATTACAGCGTTTTTATTGTTGT	138.48592
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29188_Cjap_Sim2018_COI	ADL-A16	1	BD29188_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29188_Esin_And2018_Cyb	ADL-A16	1	BD29188_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A16	Cytb	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	ACCCCTCCTCATATCCAACCA	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29188_Mgig_Bot2012_COI	ADL-A16	1	BD29188_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	TCTTATTCGTTGGAGACTTTATAACCCT	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29188_Mjap_Gib2022_COI	ADL-A16	1	BD29188_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	AATTTAATTGTGACTAGGCATGGTCTG	138.48413
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.419	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29189_Cmae_Bot2011_COI	ADL-A15	1	BD29189_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	ATGAACAGTCTATCCTCCTTTAG	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29189_Hsan_Wil2021_COI	ADL-A15	1	BD29189_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	CCTGGGCCGGTATAGTAGGT	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29189_Mros_Gib2022_COI	ADL-A15	1	BD29189_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29189_Msal_Bot2012_COI	ADL-A15	1	BD29189_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	GYTAGTTCCRATGATGTTAGCTG	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29189_Mstr_Wil2021_COI	ADL-A15	1	BD29189_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	GTAGTGGTAACTACTCATGCTTTA	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29189_Pvir_Dia2013_COI	ADL-A15	1	BD29189_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	CTTAGTGGCATTAATTCGDAATCC	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29189_Rhar_Sim2018_COI	ADL-A15	1	BD29189_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	CTGCTGCTATTGCTCATG	138.48498
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29190_Aamu_Bax2006_COI	ADL-A14	1	BD29190_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	GCACAACCGGGATCTTTACTTC	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29190_Pcan_Bot2011_IGS	ADL-A14	1	BD29190_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A14	IGS	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	CGTAATCCTCAGTACTGGCTA	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29190_Pper_Dia2013_COI	ADL-A14	1	BD29190_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	CTTAGTGGCATTAATTCGDAATCC	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.946	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29190_Sspa_Oph2007_COI	ADL-A14	1	BD29190_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	GAAGGCCTTCCGATGATC	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29190_Upin_Bot2011_COI	ADL-A14	1	BD29190_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	CTTTAATTACAGCGTTTTTATTGTTGT	138.48294
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29191_Cjap_Sim2018_COI	ADL-A13	1	BD29191_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29191_Esin_And2018_Cyb	ADL-A13	1	BD29191_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A13	Cytb	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	ACCCCTCCTCATATCCAACCA	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29191_Mgig_Bot2012_COI	ADL-A13	1	BD29191_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	TCTTATTCGTTGGAGACTTTATAACCCT	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29191_Mjap_Gib2022_COI	ADL-A13	1	BD29191_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	AATTTAATTGTGACTAGGCATGGTCTG	138.48374
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29192_Cmae_Bot2011_COI	ADL-A12	1	BD29192_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	ATGAACAGTCTATCCTCCTTTAG	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29192_Hsan_Wil2021_COI	ADL-A12	1	BD29192_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	CCTGGGCCGGTATAGTAGGT	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29192_Mros_Gib2022_COI	ADL-A12	1	BD29192_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29192_Msal_Bot2012_COI	ADL-A12	1	BD29192_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	GYTAGTTCCRATGATGTTAGCTG	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29192_Mstr_Wil2021_COI	ADL-A12	1	BD29192_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	GTAGTGGTAACTACTCATGCTTTA	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29192_Pvir_Dia2013_COI	ADL-A12	1	BD29192_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	CTTAGTGGCATTAATTCGDAATCC	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29192_Rhar_Sim2018_COI	ADL-A12	1	BD29192_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	CTGCTGCTATTGCTCATG	138.48197
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29194_Aamu_Bax2006_COI	ADL-A10	1	BD29194_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	GCACAACCGGGATCTTTACTTC	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29194_Pcan_Bot2011_IGS	ADL-A10	1	BD29194_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A10	IGS	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	CGTAATCCTCAGTACTGGCTA	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29194_Pper_Dia2013_COI	ADL-A10	1	BD29194_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	CTTAGTGGCATTAATTCGDAATCC	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.635	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29194_Sspa_Oph2007_COI	ADL-A10	1	BD29194_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	GAAGGCCTTCCGATGATC	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29194_Upin_Bot2011_COI	ADL-A10	1	BD29194_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	CTTTAATTACAGCGTTTTTATTGTTGT	138.4815
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29195_Cjap_Sim2018_COI	ADL-A09	1	BD29195_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29195_Esin_And2018_Cyb	ADL-A09	1	BD29195_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A09	Cytb	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	ACCCCTCCTCATATCCAACCA	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29195_Mgig_Bot2012_COI	ADL-A09	1	BD29195_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	TCTTATTCGTTGGAGACTTTATAACCCT	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29195_Mjap_Gib2022_COI	ADL-A09	1	BD29195_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	AATTTAATTGTGACTAGGCATGGTCTG	138.48218
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.543	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29196_Cmae_Bot2011_COI	ADL-A08	1	BD29196_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	ATGAACAGTCTATCCTCCTTTAG	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29196_Hsan_Wil2021_COI	ADL-A08	1	BD29196_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	CCTGGGCCGGTATAGTAGGT	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29196_Mros_Gib2022_COI	ADL-A08	1	BD29196_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29196_Msal_Bot2012_COI	ADL-A08	1	BD29196_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	GYTAGTTCCRATGATGTTAGCTG	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29196_Mstr_Wil2021_COI	ADL-A08	1	BD29196_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	GTAGTGGTAACTACTCATGCTTTA	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29196_Pvir_Dia2013_COI	ADL-A08	1	BD29196_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	CTTAGTGGCATTAATTCGDAATCC	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29196_Rhar_Sim2018_COI	ADL-A08	1	BD29196_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	CTGCTGCTATTGCTCATG	138.47978
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29198_Aamu_Bax2006_COI	ADL-A06	1	BD29198_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	GCACAACCGGGATCTTTACTTC	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29198_Pcan_Bot2011_IGS	ADL-A06	1	BD29198_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A06	IGS	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	CGTAATCCTCAGTACTGGCTA	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29198_Pper_Dia2013_COI	ADL-A06	1	BD29198_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	CTTAGTGGCATTAATTCGDAATCC	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.356	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29198_Sspa_Oph2007_COI	ADL-A06	1	BD29198_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	GAAGGCCTTCCGATGATC	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29198_Upin_Bot2011_COI	ADL-A06	1	BD29198_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	CTTTAATTACAGCGTTTTTATTGTTGT	138.47728
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29199_Cjap_Sim2018_COI	ADL-A05	1	BD29199_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29199_Esin_And2018_Cyb	ADL-A05	1	BD29199_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A05	Cytb	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	ACCCCTCCTCATATCCAACCA	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29199_Mgig_Bot2012_COI	ADL-A05	1	BD29199_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	TCTTATTCGTTGGAGACTTTATAACCCT	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29199_Mjap_Gib2022_COI	ADL-A05	1	BD29199_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	AATTTAATTGTGACTAGGCATGGTCTG	138.47665
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.276	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29200_Cmae_Bot2011_COI	ADL-A04	1	BD29200_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	ATGAACAGTCTATCCTCCTTTAG	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29200_Hsan_Wil2021_COI	ADL-A04	1	BD29200_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	CCTGGGCCGGTATAGTAGGT	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29200_Mros_Gib2022_COI	ADL-A04	1	BD29200_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29200_Msal_Bot2012_COI	ADL-A04	1	BD29200_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	GYTAGTTCCRATGATGTTAGCTG	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29200_Mstr_Wil2021_COI	ADL-A04	1	BD29200_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	GTAGTGGTAACTACTCATGCTTTA	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29200_Pvir_Dia2013_COI	ADL-A04	1	BD29200_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	CTTAGTGGCATTAATTCGDAATCC	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29200_Rhar_Sim2018_COI	ADL-A04	1	BD29200_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	CTGCTGCTATTGCTCATG	138.47562
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29202_Aamu_Bax2006_COI	ADL-A02	1	BD29202_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	GCACAACCGGGATCTTTACTTC	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29202_Pcan_Bot2011_IGS	ADL-A02	1	BD29202_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A02	IGS	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	CGTAATCCTCAGTACTGGCTA	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29202_Pper_Dia2013_COI	ADL-A02	1	BD29202_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	CTTAGTGGCATTAATTCGDAATCC	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29202_Sspa_Oph2007_COI	ADL-A02	1	BD29202_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	GAAGGCCTTCCGATGATC	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29202_Upin_Bot2011_COI	ADL-A02	1	BD29202_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	CTTTAATTACAGCGTTTTTATTGTTGT	138.47521
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29203_Cjap_Sim2018_COI	ADL-A01	1	BD29203_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29203_Esin_And2018_Cyb	ADL-A01	1	BD29203_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A01	Cytb	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	ACCCCTCCTCATATCCAACCA	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29203_Mgig_Bot2012_COI	ADL-A01	1	BD29203_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	TCTTATTCGTTGGAGACTTTATAACCCT	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29203_Mjap_Gib2022_COI	ADL-A01	1	BD29203_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	AATTTAATTGTGACTAGGCATGGTCTG	138.47237
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29210_Cjap_Sim2018_COI	THE-A34	1	BD29210_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29210_Esin_And2018_Cyb	THE-A34	1	BD29210_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A34	Cytb	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	ACCCCTCCTCATATCCAACCA	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29210_Mgig_Bot2012_COI	THE-A34	1	BD29210_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	TCTTATTCGTTGGAGACTTTATAACCCT	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29210_Mjap_Gib2022_COI	THE-A34	1	BD29210_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	AATTTAATTGTGACTAGGCATGGTCTG	133.63997
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29211_Cmae_Bot2011_COI	THE-A33	1	BD29211_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	ATGAACAGTCTATCCTCCTTTAG	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29211_Hsan_Wil2021_COI	THE-A33	1	BD29211_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	CCTGGGCCGGTATAGTAGGT	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29211_Mros_Gib2022_COI	THE-A33	1	BD29211_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29211_Msal_Bot2012_COI	THE-A33	1	BD29211_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	GYTAGTTCCRATGATGTTAGCTG	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29211_Mstr_Wil2021_COI	THE-A33	1	BD29211_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	GTAGTGGTAACTACTCATGCTTTA	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29211_Pvir_Dia2013_COI	THE-A33	1	BD29211_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	CTTAGTGGCATTAATTCGDAATCC	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29211_Rhar_Sim2018_COI	THE-A33	1	BD29211_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	CTGCTGCTATTGCTCATG	133.63854
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29213_Aamu_Bax2006_COI	THE-A31	1	BD29213_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	GCACAACCGGGATCTTTACTTC	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29213_Pcan_Bot2011_IGS	THE-A31	1	BD29213_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A31	IGS	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	CGTAATCCTCAGTACTGGCTA	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29213_Pper_Dia2013_COI	THE-A31	1	BD29213_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	CTTAGTGGCATTAATTCGDAATCC	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29213_Sspa_Oph2007_COI	THE-A31	1	BD29213_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	GAAGGCCTTCCGATGATC	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29213_Upin_Bot2011_COI	THE-A31	1	BD29213_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	CTTTAATTACAGCGTTTTTATTGTTGT	133.63489
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29214_Cjap_Sim2018_COI	THE-A30	1	BD29214_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29214_Esin_And2018_Cyb	THE-A30	1	BD29214_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A30	Cytb	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	ACCCCTCCTCATATCCAACCA	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29214_Mgig_Bot2012_COI	THE-A30	1	BD29214_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	TCTTATTCGTTGGAGACTTTATAACCCT	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29214_Mjap_Gib2022_COI	THE-A30	1	BD29214_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	AATTTAATTGTGACTAGGCATGGTCTG	133.63639
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29215_Cmae_Bot2011_COI	THE-A29	1	BD29215_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	ATGAACAGTCTATCCTCCTTTAG	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29215_Hsan_Wil2021_COI	THE-A29	1	BD29215_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	CCTGGGCCGGTATAGTAGGT	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29215_Mros_Gib2022_COI	THE-A29	1	BD29215_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29215_Msal_Bot2012_COI	THE-A29	1	BD29215_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	GYTAGTTCCRATGATGTTAGCTG	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29215_Mstr_Wil2021_COI	THE-A29	1	BD29215_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	GTAGTGGTAACTACTCATGCTTTA	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29215_Pvir_Dia2013_COI	THE-A29	1	BD29215_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	CTTAGTGGCATTAATTCGDAATCC	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29215_Rhar_Sim2018_COI	THE-A29	1	BD29215_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	CTGCTGCTATTGCTCATG	133.63786
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29217_Aamu_Bax2006_COI	THE-A27	1	BD29217_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	GCACAACCGGGATCTTTACTTC	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29217_Pcan_Bot2011_IGS	THE-A27	1	BD29217_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A27	IGS	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	CGTAATCCTCAGTACTGGCTA	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29217_Pper_Dia2013_COI	THE-A27	1	BD29217_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	CTTAGTGGCATTAATTCGDAATCC	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29217_Sspa_Oph2007_COI	THE-A27	1	BD29217_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	GAAGGCCTTCCGATGATC	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29217_Upin_Bot2011_COI	THE-A27	1	BD29217_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	CTTTAATTACAGCGTTTTTATTGTTGT	133.64061
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29218_Cjap_Sim2018_COI	THE-A26	1	BD29218_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29218_Esin_And2018_Cyb	THE-A26	1	BD29218_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A26	Cytb	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	ACCCCTCCTCATATCCAACCA	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29218_Mgig_Bot2012_COI	THE-A26	1	BD29218_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	TCTTATTCGTTGGAGACTTTATAACCCT	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29218_Mjap_Gib2022_COI	THE-A26	1	BD29218_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	AATTTAATTGTGACTAGGCATGGTCTG	133.64141
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29219_Cmae_Bot2011_COI	THE-A25	1	BD29219_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	ATGAACAGTCTATCCTCCTTTAG	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29219_Hsan_Wil2021_COI	THE-A25	1	BD29219_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	CCTGGGCCGGTATAGTAGGT	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29219_Mros_Gib2022_COI	THE-A25	1	BD29219_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29219_Msal_Bot2012_COI	THE-A25	1	BD29219_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	GYTAGTTCCRATGATGTTAGCTG	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29219_Mstr_Wil2021_COI	THE-A25	1	BD29219_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	GTAGTGGTAACTACTCATGCTTTA	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29219_Pvir_Dia2013_COI	THE-A25	1	BD29219_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	CTTAGTGGCATTAATTCGDAATCC	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29219_Rhar_Sim2018_COI	THE-A25	1	BD29219_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	CTGCTGCTATTGCTCATG	133.64001
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29220_Aamu_Bax2006_COI	THE-A24	1	BD29220_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	GCACAACCGGGATCTTTACTTC	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29220_Pcan_Bot2011_IGS	THE-A24	1	BD29220_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A24	IGS	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	CGTAATCCTCAGTACTGGCTA	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29220_Pper_Dia2013_COI	THE-A24	1	BD29220_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	CTTAGTGGCATTAATTCGDAATCC	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29220_Sspa_Oph2007_COI	THE-A24	1	BD29220_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	GAAGGCCTTCCGATGATC	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29220_Upin_Bot2011_COI	THE-A24	1	BD29220_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	CTTTAATTACAGCGTTTTTATTGTTGT	133.63854
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29221_Cjap_Sim2018_COI	THE-A23	1	BD29221_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29221_Esin_And2018_Cyb	THE-A23	1	BD29221_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A23	Cytb	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	ACCCCTCCTCATATCCAACCA	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29221_Mgig_Bot2012_COI	THE-A23	1	BD29221_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	TCTTATTCGTTGGAGACTTTATAACCCT	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29221_Mjap_Gib2022_COI	THE-A23	1	BD29221_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	AATTTAATTGTGACTAGGCATGGTCTG	133.63701
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29222_Cmae_Bot2011_COI	THE-A22	1	BD29222_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	ATGAACAGTCTATCCTCCTTTAG	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29222_Hsan_Wil2021_COI	THE-A22	1	BD29222_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	CCTGGGCCGGTATAGTAGGT	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29222_Mros_Gib2022_COI	THE-A22	1	BD29222_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29222_Msal_Bot2012_COI	THE-A22	1	BD29222_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	GYTAGTTCCRATGATGTTAGCTG	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29222_Mstr_Wil2021_COI	THE-A22	1	BD29222_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	GTAGTGGTAACTACTCATGCTTTA	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29222_Pvir_Dia2013_COI	THE-A22	1	BD29222_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	CTTAGTGGCATTAATTCGDAATCC	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29222_Rhar_Sim2018_COI	THE-A22	1	BD29222_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	CTGCTGCTATTGCTCATG	133.63571
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29224_Aamu_Bax2006_COI	THE-A20	1	BD29224_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	GCACAACCGGGATCTTTACTTC	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29224_Pcan_Bot2011_IGS	THE-A20	1	BD29224_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A20	IGS	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	CGTAATCCTCAGTACTGGCTA	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29224_Pper_Dia2013_COI	THE-A20	1	BD29224_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	CTTAGTGGCATTAATTCGDAATCC	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29224_Sspa_Oph2007_COI	THE-A20	1	BD29224_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	GAAGGCCTTCCGATGATC	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29224_Upin_Bot2011_COI	THE-A20	1	BD29224_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	CTTTAATTACAGCGTTTTTATTGTTGT	133.63631
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29225_Cjap_Sim2018_COI	THE-A19	1	BD29225_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29225_Esin_And2018_Cyb	THE-A19	1	BD29225_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A19	Cytb	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	ACCCCTCCTCATATCCAACCA	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29225_Mgig_Bot2012_COI	THE-A19	1	BD29225_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	TCTTATTCGTTGGAGACTTTATAACCCT	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29225_Mjap_Gib2022_COI	THE-A19	1	BD29225_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	AATTTAATTGTGACTAGGCATGGTCTG	133.63764
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29226_Cmae_Bot2011_COI	THE-A18	1	BD29226_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	ATGAACAGTCTATCCTCCTTTAG	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29226_Hsan_Wil2021_COI	THE-A18	1	BD29226_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	CCTGGGCCGGTATAGTAGGT	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29226_Mros_Gib2022_COI	THE-A18	1	BD29226_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29226_Msal_Bot2012_COI	THE-A18	1	BD29226_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	GYTAGTTCCRATGATGTTAGCTG	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29226_Mstr_Wil2021_COI	THE-A18	1	BD29226_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	GTAGTGGTAACTACTCATGCTTTA	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29226_Pvir_Dia2013_COI	THE-A18	1	BD29226_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	CTTAGTGGCATTAATTCGDAATCC	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29226_Rhar_Sim2018_COI	THE-A18	1	BD29226_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	CTGCTGCTATTGCTCATG	133.6378
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29228_Aamu_Bax2006_COI	THE-A16	1	BD29228_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	GCACAACCGGGATCTTTACTTC	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29228_Pcan_Bot2011_IGS	THE-A16	1	BD29228_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A16	IGS	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	CGTAATCCTCAGTACTGGCTA	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29228_Pper_Dia2013_COI	THE-A16	1	BD29228_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	CTTAGTGGCATTAATTCGDAATCC	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29228_Sspa_Oph2007_COI	THE-A16	1	BD29228_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	GAAGGCCTTCCGATGATC	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29228_Upin_Bot2011_COI	THE-A16	1	BD29228_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	CTTTAATTACAGCGTTTTTATTGTTGT	133.64079
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29229_Cjap_Sim2018_COI	THE-A15	1	BD29229_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29229_Esin_And2018_Cyb	THE-A15	1	BD29229_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A15	Cytb	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	ACCCCTCCTCATATCCAACCA	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29229_Mgig_Bot2012_COI	THE-A15	1	BD29229_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	TCTTATTCGTTGGAGACTTTATAACCCT	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29229_Mjap_Gib2022_COI	THE-A15	1	BD29229_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	AATTTAATTGTGACTAGGCATGGTCTG	133.63994
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29231_Aamu_Bax2006_COI	THE-A13	1	BD29231_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	GCACAACCGGGATCTTTACTTC	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29231_Pcan_Bot2011_IGS	THE-A13	1	BD29231_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A13	IGS	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	CGTAATCCTCAGTACTGGCTA	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29231_Pper_Dia2013_COI	THE-A13	1	BD29231_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	CTTAGTGGCATTAATTCGDAATCC	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29231_Sspa_Oph2007_COI	THE-A13	1	BD29231_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	GAAGGCCTTCCGATGATC	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29231_Upin_Bot2011_COI	THE-A13	1	BD29231_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	CTTTAATTACAGCGTTTTTATTGTTGT	133.63711
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29232_Cjap_Sim2018_COI	THE-A11	1	BD29232_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29232_Esin_And2018_Cyb	THE-A11	1	BD29232_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A11	Cytb	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	ACCCCTCCTCATATCCAACCA	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29232_Mgig_Bot2012_COI	THE-A11	1	BD29232_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	TCTTATTCGTTGGAGACTTTATAACCCT	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29232_Mjap_Gib2022_COI	THE-A11	1	BD29232_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	AATTTAATTGTGACTAGGCATGGTCTG	133.6349
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29233_Cmae_Bot2011_COI	THE-A12	1	BD29233_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	ATGAACAGTCTATCCTCCTTTAG	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29233_Hsan_Wil2021_COI	THE-A12	1	BD29233_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	CCTGGGCCGGTATAGTAGGT	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29233_Mros_Gib2022_COI	THE-A12	1	BD29233_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29233_Msal_Bot2012_COI	THE-A12	1	BD29233_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	GYTAGTTCCRATGATGTTAGCTG	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29233_Mstr_Wil2021_COI	THE-A12	1	BD29233_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	GTAGTGGTAACTACTCATGCTTTA	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29233_Pvir_Dia2013_COI	THE-A12	1	BD29233_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	CTTAGTGGCATTAATTCGDAATCC	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29233_Rhar_Sim2018_COI	THE-A12	1	BD29233_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	CTGCTGCTATTGCTCATG	133.63562
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29235_Aamu_Bax2006_COI	THE-A09	1	BD29235_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	GCACAACCGGGATCTTTACTTC	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29235_Pcan_Bot2011_IGS	THE-A09	1	BD29235_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A09	IGS	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	CGTAATCCTCAGTACTGGCTA	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29235_Pper_Dia2013_COI	THE-A09	1	BD29235_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	CTTAGTGGCATTAATTCGDAATCC	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29235_Sspa_Oph2007_COI	THE-A09	1	BD29235_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	GAAGGCCTTCCGATGATC	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29235_Upin_Bot2011_COI	THE-A09	1	BD29235_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	CTTTAATTACAGCGTTTTTATTGTTGT	133.63786
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29236_Cjap_Sim2018_COI	THE-A08	1	BD29236_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29236_Esin_And2018_Cyb	THE-A08	1	BD29236_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A08	Cytb	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	ACCCCTCCTCATATCCAACCA	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29236_Mgig_Bot2012_COI	THE-A08	1	BD29236_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	TCTTATTCGTTGGAGACTTTATAACCCT	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29236_Mjap_Gib2022_COI	THE-A08	1	BD29236_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	AATTTAATTGTGACTAGGCATGGTCTG	133.63907
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29237_Cmae_Bot2011_COI	THE-A07	1	BD29237_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	ATGAACAGTCTATCCTCCTTTAG	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29237_Hsan_Wil2021_COI	THE-A07	1	BD29237_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	CCTGGGCCGGTATAGTAGGT	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29237_Mros_Gib2022_COI	THE-A07	1	BD29237_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29237_Msal_Bot2012_COI	THE-A07	1	BD29237_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	GYTAGTTCCRATGATGTTAGCTG	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29237_Mstr_Wil2021_COI	THE-A07	1	BD29237_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	GTAGTGGTAACTACTCATGCTTTA	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29237_Pvir_Dia2013_COI	THE-A07	1	BD29237_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	CTTAGTGGCATTAATTCGDAATCC	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29237_Rhar_Sim2018_COI	THE-A07	1	BD29237_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	CTGCTGCTATTGCTCATG	133.64068
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29239_Aamu_Bax2006_COI	THE-A05	1	BD29239_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	GCACAACCGGGATCTTTACTTC	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29239_Pcan_Bot2011_IGS	THE-A05	1	BD29239_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A05	IGS	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	CGTAATCCTCAGTACTGGCTA	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29239_Pper_Dia2013_COI	THE-A05	1	BD29239_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	CTTAGTGGCATTAATTCGDAATCC	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29239_Sspa_Oph2007_COI	THE-A05	1	BD29239_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	GAAGGCCTTCCGATGATC	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29239_Upin_Bot2011_COI	THE-A05	1	BD29239_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	CTTTAATTACAGCGTTTTTATTGTTGT	133.64142
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29240_Cmae_Bot2011_COI	THE-A04	1	BD29240_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	ATGAACAGTCTATCCTCCTTTAG	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29240_Hsan_Wil2021_COI	THE-A04	1	BD29240_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	CCTGGGCCGGTATAGTAGGT	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29240_Mros_Gib2022_COI	THE-A04	1	BD29240_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29240_Msal_Bot2012_COI	THE-A04	1	BD29240_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	GYTAGTTCCRATGATGTTAGCTG	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29240_Mstr_Wil2021_COI	THE-A04	1	BD29240_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	GTAGTGGTAACTACTCATGCTTTA	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29240_Pvir_Dia2013_COI	THE-A04	1	BD29240_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	CTTAGTGGCATTAATTCGDAATCC	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29240_Rhar_Sim2018_COI	THE-A04	1	BD29240_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	CTGCTGCTATTGCTCATG	133.63986
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29242_Aamu_Bax2006_COI	THE-A02	1	BD29242_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	GCACAACCGGGATCTTTACTTC	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29242_Pcan_Bot2011_IGS	THE-A02	1	BD29242_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A02	IGS	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	CGTAATCCTCAGTACTGGCTA	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29242_Pper_Dia2013_COI	THE-A02	1	BD29242_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	CTTAGTGGCATTAATTCGDAATCC	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29242_Sspa_Oph2007_COI	THE-A02	1	BD29242_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	GAAGGCCTTCCGATGATC	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29242_Upin_Bot2011_COI	THE-A02	1	BD29242_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	CTTTAATTACAGCGTTTTTATTGTTGT	133.6372
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29243_Cjap_Sim2018_COI	THE-A01	1	BD29243_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29243_Esin_And2018_Cyb	THE-A01	1	BD29243_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A01	Cytb	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	ACCCCTCCTCATATCCAACCA	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29243_Mgig_Bot2012_COI	THE-A01	1	BD29243_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	TCTTATTCGTTGGAGACTTTATAACCCT	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29243_Mjap_Gib2022_COI	THE-A01	1	BD29243_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	AATTTAATTGTGACTAGGCATGGTCTG	133.63322
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29244_Cmae_Bot2011_COI	PLO-A40	1	BD29244_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	ATGAACAGTCTATCCTCCTTTAG	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29244_Hsan_Wil2021_COI	PLO-A40	1	BD29244_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	CCTGGGCCGGTATAGTAGGT	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29244_Mros_Gib2022_COI	PLO-A40	1	BD29244_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29244_Msal_Bot2012_COI	PLO-A40	1	BD29244_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	GYTAGTTCCRATGATGTTAGCTG	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29244_Mstr_Wil2021_COI	PLO-A40	1	BD29244_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	GTAGTGGTAACTACTCATGCTTTA	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29244_Pvir_Dia2013_COI	PLO-A40	1	BD29244_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	CTTAGTGGCATTAATTCGDAATCC	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29244_Rhar_Sim2018_COI	PLO-A40	1	BD29244_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	CTGCTGCTATTGCTCATG	135.87311
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29246_Aamu_Bax2006_COI	PLO-A38	1	BD29246_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	GCACAACCGGGATCTTTACTTC	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29246_Pcan_Bot2011_IGS	PLO-A38	1	BD29246_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A38	IGS	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	CGTAATCCTCAGTACTGGCTA	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29246_Pper_Dia2013_COI	PLO-A38	1	BD29246_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	CTTAGTGGCATTAATTCGDAATCC	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.971	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29246_Sspa_Oph2007_COI	PLO-A38	1	BD29246_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	GAAGGCCTTCCGATGATC	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29246_Upin_Bot2011_COI	PLO-A38	1	BD29246_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	CTTTAATTACAGCGTTTTTATTGTTGT	135.87366
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29247_Cjap_Sim2018_COI	PLO-A37	1	BD29247_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29247_Esin_And2018_Cyb	PLO-A37	1	BD29247_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A37	Cytb	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	ACCCCTCCTCATATCCAACCA	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29247_Mgig_Bot2012_COI	PLO-A37	1	BD29247_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	TCTTATTCGTTGGAGACTTTATAACCCT	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29247_Mjap_Gib2022_COI	PLO-A37	1	BD29247_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	AATTTAATTGTGACTAGGCATGGTCTG	135.87481
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29248_Cmae_Bot2011_COI	PLO-A36	1	BD29248_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	ATGAACAGTCTATCCTCCTTTAG	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29248_Hsan_Wil2021_COI	PLO-A36	1	BD29248_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	CCTGGGCCGGTATAGTAGGT	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29248_Mros_Gib2022_COI	PLO-A36	1	BD29248_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29248_Msal_Bot2012_COI	PLO-A36	1	BD29248_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	GYTAGTTCCRATGATGTTAGCTG	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29248_Mstr_Wil2021_COI	PLO-A36	1	BD29248_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	GTAGTGGTAACTACTCATGCTTTA	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29248_Pvir_Dia2013_COI	PLO-A36	1	BD29248_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	CTTAGTGGCATTAATTCGDAATCC	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29248_Rhar_Sim2018_COI	PLO-A36	1	BD29248_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	CTGCTGCTATTGCTCATG	135.87696
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29250_Cjap_Sim2018_COI	PLO-A34	1	BD29250_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29250_Esin_And2018_Cyb	PLO-A34	1	BD29250_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A34	Cytb	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	ACCCCTCCTCATATCCAACCA	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29250_Mgig_Bot2012_COI	PLO-A34	1	BD29250_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	TCTTATTCGTTGGAGACTTTATAACCCT	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29250_Mjap_Gib2022_COI	PLO-A34	1	BD29250_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	AATTTAATTGTGACTAGGCATGGTCTG	135.8746
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29251_Cmae_Bot2011_COI	PLO-A33	1	BD29251_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	ATGAACAGTCTATCCTCCTTTAG	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29251_Hsan_Wil2021_COI	PLO-A33	1	BD29251_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	CCTGGGCCGGTATAGTAGGT	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29251_Mros_Gib2022_COI	PLO-A33	1	BD29251_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29251_Msal_Bot2012_COI	PLO-A33	1	BD29251_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	GYTAGTTCCRATGATGTTAGCTG	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29251_Mstr_Wil2021_COI	PLO-A33	1	BD29251_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	GTAGTGGTAACTACTCATGCTTTA	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29251_Pvir_Dia2013_COI	PLO-A33	1	BD29251_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	CTTAGTGGCATTAATTCGDAATCC	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29251_Rhar_Sim2018_COI	PLO-A33	1	BD29251_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	CTGCTGCTATTGCTCATG	135.87175
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29253_Aamu_Bax2006_COI	PLO-A31	1	BD29253_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	GCACAACCGGGATCTTTACTTC	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29253_Pcan_Bot2011_IGS	PLO-A31	1	BD29253_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A31	IGS	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	CGTAATCCTCAGTACTGGCTA	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29253_Pper_Dia2013_COI	PLO-A31	1	BD29253_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	CTTAGTGGCATTAATTCGDAATCC	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.042	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29253_Sspa_Oph2007_COI	PLO-A31	1	BD29253_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	GAAGGCCTTCCGATGATC	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29253_Upin_Bot2011_COI	PLO-A31	1	BD29253_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	CTTTAATTACAGCGTTTTTATTGTTGT	135.87589
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29254_Cjap_Sim2018_COI	PLO-A30	1	BD29254_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29254_Esin_And2018_Cyb	PLO-A30	1	BD29254_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A30	Cytb	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	ACCCCTCCTCATATCCAACCA	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29254_Mgig_Bot2012_COI	PLO-A30	1	BD29254_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	TCTTATTCGTTGGAGACTTTATAACCCT	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29254_Mjap_Gib2022_COI	PLO-A30	1	BD29254_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	AATTTAATTGTGACTAGGCATGGTCTG	135.87692
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29255_Cmae_Bot2011_COI	PLO-A29	1	BD29255_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	ATGAACAGTCTATCCTCCTTTAG	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29255_Hsan_Wil2021_COI	PLO-A29	1	BD29255_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	CCTGGGCCGGTATAGTAGGT	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29255_Mros_Gib2022_COI	PLO-A29	1	BD29255_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29255_Msal_Bot2012_COI	PLO-A29	1	BD29255_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	GYTAGTTCCRATGATGTTAGCTG	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29255_Mstr_Wil2021_COI	PLO-A29	1	BD29255_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	GTAGTGGTAACTACTCATGCTTTA	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29255_Pvir_Dia2013_COI	PLO-A29	1	BD29255_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	CTTAGTGGCATTAATTCGDAATCC	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29255_Rhar_Sim2018_COI	PLO-A29	1	BD29255_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	CTGCTGCTATTGCTCATG	135.87799
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29257_Aamu_Bax2006_COI	PLO-A27	1	BD29257_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	GCACAACCGGGATCTTTACTTC	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29257_Pcan_Bot2011_IGS	PLO-A27	1	BD29257_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A27	IGS	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	CGTAATCCTCAGTACTGGCTA	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29257_Pper_Dia2013_COI	PLO-A27	1	BD29257_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	CTTAGTGGCATTAATTCGDAATCC	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.523	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29257_Sspa_Oph2007_COI	PLO-A27	1	BD29257_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	GAAGGCCTTCCGATGATC	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29257_Upin_Bot2011_COI	PLO-A27	1	BD29257_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	CTTTAATTACAGCGTTTTTATTGTTGT	135.87467
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29258_Cjap_Sim2018_COI	PLO-A26	1	BD29258_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29258_Esin_And2018_Cyb	PLO-A26	1	BD29258_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A26	Cytb	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	ACCCCTCCTCATATCCAACCA	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29258_Mgig_Bot2012_COI	PLO-A26	1	BD29258_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	TCTTATTCGTTGGAGACTTTATAACCCT	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29258_Mjap_Gib2022_COI	PLO-A26	1	BD29258_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	AATTTAATTGTGACTAGGCATGGTCTG	135.87367
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29259_Cmae_Bot2011_COI	PLO-A25	1	BD29259_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	ATGAACAGTCTATCCTCCTTTAG	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29259_Hsan_Wil2021_COI	PLO-A25	1	BD29259_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	CCTGGGCCGGTATAGTAGGT	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29259_Mros_Gib2022_COI	PLO-A25	1	BD29259_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29259_Msal_Bot2012_COI	PLO-A25	1	BD29259_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	GYTAGTTCCRATGATGTTAGCTG	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29259_Mstr_Wil2021_COI	PLO-A25	1	BD29259_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	GTAGTGGTAACTACTCATGCTTTA	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29259_Pvir_Dia2013_COI	PLO-A25	1	BD29259_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	CTTAGTGGCATTAATTCGDAATCC	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29259_Rhar_Sim2018_COI	PLO-A25	1	BD29259_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	CTGCTGCTATTGCTCATG	135.87256
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29260_Aamu_Bax2006_COI	PLO-A22	1	BD29260_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	GCACAACCGGGATCTTTACTTC	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29260_Pcan_Bot2011_IGS	PLO-A22	1	BD29260_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A22	IGS	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	CGTAATCCTCAGTACTGGCTA	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29260_Pper_Dia2013_COI	PLO-A22	1	BD29260_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	CTTAGTGGCATTAATTCGDAATCC	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.07	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29260_Sspa_Oph2007_COI	PLO-A22	1	BD29260_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	GAAGGCCTTCCGATGATC	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29260_Upin_Bot2011_COI	PLO-A22	1	BD29260_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	CTTTAATTACAGCGTTTTTATTGTTGT	135.86801
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29261_Cjap_Sim2018_COI	PLO-A24	1	BD29261_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29261_Esin_And2018_Cyb	PLO-A24	1	BD29261_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A24	Cytb	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	ACCCCTCCTCATATCCAACCA	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29261_Mgig_Bot2012_COI	PLO-A24	1	BD29261_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	TCTTATTCGTTGGAGACTTTATAACCCT	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29261_Mjap_Gib2022_COI	PLO-A24	1	BD29261_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	AATTTAATTGTGACTAGGCATGGTCTG	135.87139
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29262_Cmae_Bot2011_COI	PLO-A23	1	BD29262_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	ATGAACAGTCTATCCTCCTTTAG	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29262_Hsan_Wil2021_COI	PLO-A23	1	BD29262_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	CCTGGGCCGGTATAGTAGGT	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29262_Mros_Gib2022_COI	PLO-A23	1	BD29262_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29262_Msal_Bot2012_COI	PLO-A23	1	BD29262_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	GYTAGTTCCRATGATGTTAGCTG	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29262_Mstr_Wil2021_COI	PLO-A23	1	BD29262_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	GTAGTGGTAACTACTCATGCTTTA	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29262_Pvir_Dia2013_COI	PLO-A23	1	BD29262_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	CTTAGTGGCATTAATTCGDAATCC	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29262_Rhar_Sim2018_COI	PLO-A23	1	BD29262_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	CTGCTGCTATTGCTCATG	135.87022
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29264_Aamu_Bax2006_COI	PLO-A21	1	BD29264_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	GCACAACCGGGATCTTTACTTC	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29264_Pcan_Bot2011_IGS	PLO-A21	1	BD29264_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A21	IGS	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	CGTAATCCTCAGTACTGGCTA	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29264_Pper_Dia2013_COI	PLO-A21	1	BD29264_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	CTTAGTGGCATTAATTCGDAATCC	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.026	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29264_Sspa_Oph2007_COI	PLO-A21	1	BD29264_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	GAAGGCCTTCCGATGATC	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29264_Upin_Bot2011_COI	PLO-A21	1	BD29264_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	CTTTAATTACAGCGTTTTTATTGTTGT	135.86904
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29265_Cjap_Sim2018_COI	PLO-A20	1	BD29265_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29265_Esin_And2018_Cyb	PLO-A20	1	BD29265_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A20	Cytb	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	ACCCCTCCTCATATCCAACCA	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29265_Mgig_Bot2012_COI	PLO-A20	1	BD29265_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	TCTTATTCGTTGGAGACTTTATAACCCT	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29265_Mjap_Gib2022_COI	PLO-A20	1	BD29265_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	AATTTAATTGTGACTAGGCATGGTCTG	135.86686
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29266_Cmae_Bot2011_COI	PLO-A19	1	BD29266_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	ATGAACAGTCTATCCTCCTTTAG	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29266_Hsan_Wil2021_COI	PLO-A19	1	BD29266_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	CCTGGGCCGGTATAGTAGGT	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29266_Mros_Gib2022_COI	PLO-A19	1	BD29266_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29266_Msal_Bot2012_COI	PLO-A19	1	BD29266_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	GYTAGTTCCRATGATGTTAGCTG	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29266_Mstr_Wil2021_COI	PLO-A19	1	BD29266_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	GTAGTGGTAACTACTCATGCTTTA	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29266_Pvir_Dia2013_COI	PLO-A19	1	BD29266_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	CTTAGTGGCATTAATTCGDAATCC	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29266_Rhar_Sim2018_COI	PLO-A19	1	BD29266_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	CTGCTGCTATTGCTCATG	135.86574
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29268_Aamu_Bax2006_COI	PLO-A17	1	BD29268_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	GCACAACCGGGATCTTTACTTC	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29268_Pcan_Bot2011_IGS	PLO-A17	1	BD29268_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A17	IGS	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	CGTAATCCTCAGTACTGGCTA	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29268_Pper_Dia2013_COI	PLO-A17	1	BD29268_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	CTTAGTGGCATTAATTCGDAATCC	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.25	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29268_Sspa_Oph2007_COI	PLO-A17	1	BD29268_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	GAAGGCCTTCCGATGATC	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29268_Upin_Bot2011_COI	PLO-A17	1	BD29268_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	CTTTAATTACAGCGTTTTTATTGTTGT	135.8648
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29269_Cjap_Sim2018_COI	PLO-A16	1	BD29269_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29269_Esin_And2018_Cyb	PLO-A16	1	BD29269_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A16	Cytb	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	ACCCCTCCTCATATCCAACCA	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29269_Mgig_Bot2012_COI	PLO-A16	1	BD29269_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	TCTTATTCGTTGGAGACTTTATAACCCT	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29269_Mjap_Gib2022_COI	PLO-A16	1	BD29269_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	AATTTAATTGTGACTAGGCATGGTCTG	135.86685
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29271_Aamu_Bax2006_COI	PLO-A14	1	BD29271_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	GCACAACCGGGATCTTTACTTC	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29271_Pcan_Bot2011_IGS	PLO-A14	1	BD29271_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A14	IGS	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	CGTAATCCTCAGTACTGGCTA	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29271_Pper_Dia2013_COI	PLO-A14	1	BD29271_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	CTTAGTGGCATTAATTCGDAATCC	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.99	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29271_Sspa_Oph2007_COI	PLO-A14	1	BD29271_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	GAAGGCCTTCCGATGATC	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29271_Upin_Bot2011_COI	PLO-A14	1	BD29271_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	CTTTAATTACAGCGTTTTTATTGTTGT	135.86587
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29272_Cjap_Sim2018_COI	PLO-A13	1	BD29272_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29272_Esin_And2018_Cyb	PLO-A13	1	BD29272_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A13	Cytb	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	ACCCCTCCTCATATCCAACCA	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29272_Mgig_Bot2012_COI	PLO-A13	1	BD29272_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	TCTTATTCGTTGGAGACTTTATAACCCT	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29272_Mjap_Gib2022_COI	PLO-A13	1	BD29272_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	AATTTAATTGTGACTAGGCATGGTCTG	135.86812
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29273_Cmae_Bot2011_COI	PLO-A12	1	BD29273_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	ATGAACAGTCTATCCTCCTTTAG	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29273_Hsan_Wil2021_COI	PLO-A12	1	BD29273_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	CCTGGGCCGGTATAGTAGGT	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29273_Mros_Gib2022_COI	PLO-A12	1	BD29273_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29273_Msal_Bot2012_COI	PLO-A12	1	BD29273_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	GYTAGTTCCRATGATGTTAGCTG	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29273_Mstr_Wil2021_COI	PLO-A12	1	BD29273_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	GTAGTGGTAACTACTCATGCTTTA	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29273_Pvir_Dia2013_COI	PLO-A12	1	BD29273_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	CTTAGTGGCATTAATTCGDAATCC	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29273_Rhar_Sim2018_COI	PLO-A12	1	BD29273_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	CTGCTGCTATTGCTCATG	135.86717
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29275_Aamu_Bax2006_COI	PLO-A10	1	BD29275_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	GCACAACCGGGATCTTTACTTC	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29275_Pcan_Bot2011_IGS	PLO-A10	1	BD29275_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A10	IGS	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	CGTAATCCTCAGTACTGGCTA	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29275_Pper_Dia2013_COI	PLO-A10	1	BD29275_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	CTTAGTGGCATTAATTCGDAATCC	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.092	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29275_Sspa_Oph2007_COI	PLO-A10	1	BD29275_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	GAAGGCCTTCCGATGATC	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29275_Upin_Bot2011_COI	PLO-A10	1	BD29275_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	CTTTAATTACAGCGTTTTTATTGTTGT	135.8636
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29276_Cjap_Sim2018_COI	PLO-A09	1	BD29276_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29276_Esin_And2018_Cyb	PLO-A09	1	BD29276_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A09	Cytb	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	ACCCCTCCTCATATCCAACCA	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29276_Mgig_Bot2012_COI	PLO-A09	1	BD29276_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	TCTTATTCGTTGGAGACTTTATAACCCT	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29276_Mjap_Gib2022_COI	PLO-A09	1	BD29276_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	AATTTAATTGTGACTAGGCATGGTCTG	135.87123
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29277_Cmae_Bot2011_COI	PLO-A08	1	BD29277_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	ATGAACAGTCTATCCTCCTTTAG	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29277_Hsan_Wil2021_COI	PLO-A08	1	BD29277_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	CCTGGGCCGGTATAGTAGGT	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29277_Mros_Gib2022_COI	PLO-A08	1	BD29277_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29277_Msal_Bot2012_COI	PLO-A08	1	BD29277_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	GYTAGTTCCRATGATGTTAGCTG	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29277_Mstr_Wil2021_COI	PLO-A08	1	BD29277_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	GTAGTGGTAACTACTCATGCTTTA	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29277_Pvir_Dia2013_COI	PLO-A08	1	BD29277_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	CTTAGTGGCATTAATTCGDAATCC	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29277_Rhar_Sim2018_COI	PLO-A08	1	BD29277_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	CTGCTGCTATTGCTCATG	135.87218
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29279_Aamu_Bax2006_COI	PLO-A06	1	BD29279_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	GCACAACCGGGATCTTTACTTC	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29279_Pcan_Bot2011_IGS	PLO-A06	1	BD29279_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A06	IGS	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	CGTAATCCTCAGTACTGGCTA	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29279_Pper_Dia2013_COI	PLO-A06	1	BD29279_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	CTTAGTGGCATTAATTCGDAATCC	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.838	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29279_Sspa_Oph2007_COI	PLO-A06	1	BD29279_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	GAAGGCCTTCCGATGATC	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29279_Upin_Bot2011_COI	PLO-A06	1	BD29279_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	CTTTAATTACAGCGTTTTTATTGTTGT	135.8739
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29280_Cmae_Bot2011_COI	PLO-A05	1	BD29280_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	ATGAACAGTCTATCCTCCTTTAG	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29280_Hsan_Wil2021_COI	PLO-A05	1	BD29280_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	CCTGGGCCGGTATAGTAGGT	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29280_Mros_Gib2022_COI	PLO-A05	1	BD29280_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29280_Msal_Bot2012_COI	PLO-A05	1	BD29280_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	GYTAGTTCCRATGATGTTAGCTG	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29280_Mstr_Wil2021_COI	PLO-A05	1	BD29280_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	GTAGTGGTAACTACTCATGCTTTA	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29280_Pvir_Dia2013_COI	PLO-A05	1	BD29280_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	CTTAGTGGCATTAATTCGDAATCC	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29280_Rhar_Sim2018_COI	PLO-A05	1	BD29280_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	CTGCTGCTATTGCTCATG	135.87604
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29282_Aamu_Bax2006_COI	PLO-A03	1	BD29282_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	GCACAACCGGGATCTTTACTTC	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29282_Pcan_Bot2011_IGS	PLO-A03	1	BD29282_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A03	IGS	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	CGTAATCCTCAGTACTGGCTA	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29282_Pper_Dia2013_COI	PLO-A03	1	BD29282_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	CTTAGTGGCATTAATTCGDAATCC	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.376	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29282_Sspa_Oph2007_COI	PLO-A03	1	BD29282_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	GAAGGCCTTCCGATGATC	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29282_Upin_Bot2011_COI	PLO-A03	1	BD29282_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	CTTTAATTACAGCGTTTTTATTGTTGT	135.87891
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29283_Cjap_Sim2018_COI	PLO-A02	1	BD29283_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29283_Esin_And2018_Cyb	PLO-A02	1	BD29283_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A02	Cytb	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	ACCCCTCCTCATATCCAACCA	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29283_Mgig_Bot2012_COI	PLO-A02	1	BD29283_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	TCTTATTCGTTGGAGACTTTATAACCCT	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29283_Mjap_Gib2022_COI	PLO-A02	1	BD29283_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	AATTTAATTGTGACTAGGCATGGTCTG	135.87965
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29284_Cmae_Bot2011_COI	PLO-A01	1	BD29284_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	ATGAACAGTCTATCCTCCTTTAG	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29284_Hsan_Wil2021_COI	PLO-A01	1	BD29284_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	CCTGGGCCGGTATAGTAGGT	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29284_Mros_Gib2022_COI	PLO-A01	1	BD29284_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29284_Msal_Bot2012_COI	PLO-A01	1	BD29284_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	GYTAGTTCCRATGATGTTAGCTG	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29284_Mstr_Wil2021_COI	PLO-A01	1	BD29284_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	GTAGTGGTAACTACTCATGCTTTA	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29284_Pvir_Dia2013_COI	PLO-A01	1	BD29284_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	CTTAGTGGCATTAATTCGDAATCC	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29284_Rhar_Sim2018_COI	PLO-A01	1	BD29284_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	CTGCTGCTATTGCTCATG	135.8807
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29286_Aamu_Bax2006_COI	POR-A34	1	BD29286_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	GCACAACCGGGATCTTTACTTC	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29286_Pcan_Bot2011_IGS	POR-A34	1	BD29286_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A34	IGS	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	CGTAATCCTCAGTACTGGCTA	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29286_Pper_Dia2013_COI	POR-A34	1	BD29286_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	CTTAGTGGCATTAATTCGDAATCC	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.254	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29286_Sspa_Oph2007_COI	POR-A34	1	BD29286_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	GAAGGCCTTCCGATGATC	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.69	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29286_Upin_Bot2011_COI	POR-A34	1	BD29286_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Present	Sample	Upin_Bot2011_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	CTTTAATTACAGCGTTTTTATTGTTGT	141.61249
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29287_Cjap_Sim2018_COI	POR-A33	1	BD29287_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29287_Esin_And2018_Cyb	POR-A33	1	BD29287_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A33	Cytb	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	ACCCCTCCTCATATCCAACCA	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29287_Mgig_Bot2012_COI	POR-A33	1	BD29287_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	TCTTATTCGTTGGAGACTTTATAACCCT	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29287_Mjap_Gib2022_COI	POR-A33	1	BD29287_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	AATTTAATTGTGACTAGGCATGGTCTG	141.61111
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29288_Cmae_Bot2011_COI	POR-A32	1	BD29288_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	ATGAACAGTCTATCCTCCTTTAG	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29288_Hsan_Wil2021_COI	POR-A32	1	BD29288_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	CCTGGGCCGGTATAGTAGGT	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29288_Mros_Gib2022_COI	POR-A32	1	BD29288_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29288_Msal_Bot2012_COI	POR-A32	1	BD29288_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	GYTAGTTCCRATGATGTTAGCTG	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29288_Mstr_Wil2021_COI	POR-A32	1	BD29288_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	GTAGTGGTAACTACTCATGCTTTA	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29288_Pvir_Dia2013_COI	POR-A32	1	BD29288_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	CTTAGTGGCATTAATTCGDAATCC	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29288_Rhar_Sim2018_COI	POR-A32	1	BD29288_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	CTGCTGCTATTGCTCATG	141.62275
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29290_Asen_Wil2025_COI	POR-A30	1	BD29290_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	GTTGTTGTTACAACACATGCTCTTTTG	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29290_Cjap_Sim2018_COI	POR-A30	1	BD29290_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29290_Esin_And2018_Cyb	POR-A30	1	BD29290_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A30	Cytb	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	ACCCCTCCTCATATCCAACCA	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29290_Mgig_Bot2012_COI	POR-A30	1	BD29290_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	TCTTATTCGTTGGAGACTTTATAACCCT	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29290_Mjap_Gib2022_COI	POR-A30	1	BD29290_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	AATTTAATTGTGACTAGGCATGGTCTG	141.61982
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29291_Cmae_Bot2011_COI	POR-A29	1	BD29291_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	ATGAACAGTCTATCCTCCTTTAG	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29291_Hsan_Wil2021_COI	POR-A29	1	BD29291_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	CCTGGGCCGGTATAGTAGGT	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	38.78	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29291_Mros_Gib2022_COI	POR-A29	1	BD29291_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Present	Sample	Mros_Gib2022_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29291_Msal_Bot2012_COI	POR-A29	1	BD29291_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	GYTAGTTCCRATGATGTTAGCTG	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29291_Mstr_Wil2021_COI	POR-A29	1	BD29291_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	GTAGTGGTAACTACTCATGCTTTA	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29291_Pvir_Dia2013_COI	POR-A29	1	BD29291_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	CTTAGTGGCATTAATTCGDAATCC	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29291_Rhar_Sim2018_COI	POR-A29	1	BD29291_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	CTGCTGCTATTGCTCATG	141.61874
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29293_Aamu_Bax2006_COI	POR-A27	1	BD29293_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	GCACAACCGGGATCTTTACTTC	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29293_Pcan_Bot2011_IGS	POR-A27	1	BD29293_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A27	IGS	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	CGTAATCCTCAGTACTGGCTA	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29293_Pper_Dia2013_COI	POR-A27	1	BD29293_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	CTTAGTGGCATTAATTCGDAATCC	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.121	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29293_Sspa_Oph2007_COI	POR-A27	1	BD29293_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	GAAGGCCTTCCGATGATC	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.767	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29293_Upin_Bot2011_COI	POR-A27	1	BD29293_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Present	Sample	Upin_Bot2011_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	CTTTAATTACAGCGTTTTTATTGTTGT	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29293_Vgib_Wil2025_COI	POR-A27	1	BD29293_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	CCCCTCTTTACAAGTTCTTTAGGGT	141.61596
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29294_Asen_Wil2025_COI	POR-A26	1	BD29294_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	GTTGTTGTTACAACACATGCTCTTTTG	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29294_Cjap_Sim2018_COI	POR-A26	1	BD29294_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29294_Esin_And2018_Cyb	POR-A26	1	BD29294_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A26	Cytb	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	ACCCCTCCTCATATCCAACCA	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29294_Mgig_Bot2012_COI	POR-A26	1	BD29294_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	TCTTATTCGTTGGAGACTTTATAACCCT	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29294_Mjap_Gib2022_COI	POR-A26	1	BD29294_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	AATTTAATTGTGACTAGGCATGGTCTG	141.61454
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29295_Cmae_Bot2011_COI	POR-A25	1	BD29295_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	ATGAACAGTCTATCCTCCTTTAG	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29295_Hsan_Wil2021_COI	POR-A25	1	BD29295_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	CCTGGGCCGGTATAGTAGGT	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.533	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29295_Mros_Gib2022_COI	POR-A25	1	BD29295_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Present	Sample	Mros_Gib2022_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29295_Msal_Bot2012_COI	POR-A25	1	BD29295_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	GYTAGTTCCRATGATGTTAGCTG	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29295_Mstr_Wil2021_COI	POR-A25	1	BD29295_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	GTAGTGGTAACTACTCATGCTTTA	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29295_Pvir_Dia2013_COI	POR-A25	1	BD29295_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	CTTAGTGGCATTAATTCGDAATCC	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29295_Rhar_Sim2018_COI	POR-A25	1	BD29295_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	CTGCTGCTATTGCTCATG	141.61313
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29297_Aamu_Bax2006_COI	POR-A23	1	BD29297_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	GCACAACCGGGATCTTTACTTC	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29297_Pcan_Bot2011_IGS	POR-A23	1	BD29297_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A23	IGS	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	CGTAATCCTCAGTACTGGCTA	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29297_Pper_Dia2013_COI	POR-A23	1	BD29297_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	CTTAGTGGCATTAATTCGDAATCC	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29297_Sspa_Oph2007_COI	POR-A23	1	BD29297_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	GAAGGCCTTCCGATGATC	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.708	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29297_Upin_Bot2011_COI	POR-A23	1	BD29297_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Present	Sample	Upin_Bot2011_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	CTTTAATTACAGCGTTTTTATTGTTGT	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29297_Vgib_Wil2025_COI	POR-A23	1	BD29297_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	CCCCTCTTTACAAGTTCTTTAGGGT	141.62089
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29298_Asen_Wil2025_COI	POR-A22	1	BD29298_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	GTTGTTGTTACAACACATGCTCTTTTG	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29298_Cjap_Sim2018_COI	POR-A22	1	BD29298_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29298_Esin_And2018_Cyb	POR-A22	1	BD29298_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A22	Cytb	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	ACCCCTCCTCATATCCAACCA	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29298_Mgig_Bot2012_COI	POR-A22	1	BD29298_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	TCTTATTCGTTGGAGACTTTATAACCCT	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29298_Mjap_Gib2022_COI	POR-A22	1	BD29298_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	AATTTAATTGTGACTAGGCATGGTCTG	141.61961
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29299_Cmae_Bot2011_COI	POR-A21	1	BD29299_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	ATGAACAGTCTATCCTCCTTTAG	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29299_Hsan_Wil2021_COI	POR-A21	1	BD29299_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	CCTGGGCCGGTATAGTAGGT	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29299_Mros_Gib2022_COI	POR-A21	1	BD29299_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29299_Msal_Bot2012_COI	POR-A21	1	BD29299_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	GYTAGTTCCRATGATGTTAGCTG	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29299_Mstr_Wil2021_COI	POR-A21	1	BD29299_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	GTAGTGGTAACTACTCATGCTTTA	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29299_Pvir_Dia2013_COI	POR-A21	1	BD29299_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	CTTAGTGGCATTAATTCGDAATCC	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29299_Rhar_Sim2018_COI	POR-A21	1	BD29299_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	CTGCTGCTATTGCTCATG	141.61823
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29301_Aamu_Bax2006_COI	POR-A19	1	BD29301_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	GCACAACCGGGATCTTTACTTC	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29301_Pcan_Bot2011_IGS	POR-A19	1	BD29301_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A19	IGS	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	CGTAATCCTCAGTACTGGCTA	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29301_Pper_Dia2013_COI	POR-A19	1	BD29301_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	CTTAGTGGCATTAATTCGDAATCC	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.261	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29301_Sspa_Oph2007_COI	POR-A19	1	BD29301_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	GAAGGCCTTCCGATGATC	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.198	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29301_Upin_Bot2011_COI	POR-A19	1	BD29301_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Present	Sample	Upin_Bot2011_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	CTTTAATTACAGCGTTTTTATTGTTGT	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29301_Vgib_Wil2025_COI	POR-A19	1	BD29301_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	CCCCTCTTTACAAGTTCTTTAGGGT	141.6153
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29302_Asen_Wil2025_COI	POR-A18	1	BD29302_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	GTTGTTGTTACAACACATGCTCTTTTG	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29302_Cjap_Sim2018_COI	POR-A18	1	BD29302_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29302_Esin_And2018_Cyb	POR-A18	1	BD29302_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A18	Cytb	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	ACCCCTCCTCATATCCAACCA	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29302_Mgig_Bot2012_COI	POR-A18	1	BD29302_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	TCTTATTCGTTGGAGACTTTATAACCCT	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29302_Mjap_Gib2022_COI	POR-A18	1	BD29302_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	AATTTAATTGTGACTAGGCATGGTCTG	141.61393
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29303_Cmae_Bot2011_COI	POR-A17	1	BD29303_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	ATGAACAGTCTATCCTCCTTTAG	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29303_Hsan_Wil2021_COI	POR-A17	1	BD29303_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	CCTGGGCCGGTATAGTAGGT	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29303_Mros_Gib2022_COI	POR-A17	1	BD29303_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29303_Msal_Bot2012_COI	POR-A17	1	BD29303_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	GYTAGTTCCRATGATGTTAGCTG	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29303_Mstr_Wil2021_COI	POR-A17	1	BD29303_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	GTAGTGGTAACTACTCATGCTTTA	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29303_Pvir_Dia2013_COI	POR-A17	1	BD29303_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	CTTAGTGGCATTAATTCGDAATCC	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29303_Rhar_Sim2018_COI	POR-A17	1	BD29303_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	CTGCTGCTATTGCTCATG	141.61256
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29305_Aamu_Bax2006_COI	POR-A15	1	BD29305_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	GCACAACCGGGATCTTTACTTC	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29305_Pcan_Bot2011_IGS	POR-A15	1	BD29305_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A15	IGS	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	CGTAATCCTCAGTACTGGCTA	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29305_Pper_Dia2013_COI	POR-A15	1	BD29305_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	CTTAGTGGCATTAATTCGDAATCC	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29305_Sspa_Oph2007_COI	POR-A15	1	BD29305_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	GAAGGCCTTCCGATGATC	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.765	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29305_Upin_Bot2011_COI	POR-A15	1	BD29305_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Present	Sample	Upin_Bot2011_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	CTTTAATTACAGCGTTTTTATTGTTGT	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29305_Vgib_Wil2025_COI	POR-A15	1	BD29305_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	CCCCTCTTTACAAGTTCTTTAGGGT	141.6203
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29306_Asen_Wil2025_COI	POR-A14	1	BD29306_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	GTTGTTGTTACAACACATGCTCTTTTG	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29306_Cjap_Sim2018_COI	POR-A14	1	BD29306_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29306_Esin_And2018_Cyb	POR-A14	1	BD29306_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A14	Cytb	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	ACCCCTCCTCATATCCAACCA	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29306_Mgig_Bot2012_COI	POR-A14	1	BD29306_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	TCTTATTCGTTGGAGACTTTATAACCCT	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29306_Mjap_Gib2022_COI	POR-A14	1	BD29306_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	AATTTAATTGTGACTAGGCATGGTCTG	141.61892
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29307_Cmae_Bot2011_COI	POR-A13	1	BD29307_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	ATGAACAGTCTATCCTCCTTTAG	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29307_Hsan_Wil2021_COI	POR-A13	1	BD29307_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	CCTGGGCCGGTATAGTAGGT	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29307_Mros_Gib2022_COI	POR-A13	1	BD29307_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29307_Msal_Bot2012_COI	POR-A13	1	BD29307_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	GYTAGTTCCRATGATGTTAGCTG	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29307_Mstr_Wil2021_COI	POR-A13	1	BD29307_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	GTAGTGGTAACTACTCATGCTTTA	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29307_Pvir_Dia2013_COI	POR-A13	1	BD29307_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	CTTAGTGGCATTAATTCGDAATCC	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29307_Rhar_Sim2018_COI	POR-A13	1	BD29307_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	CTGCTGCTATTGCTCATG	141.61754
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29309_Aamu_Bax2006_COI	POR-A11	1	BD29309_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	GCACAACCGGGATCTTTACTTC	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29309_Pcan_Bot2011_IGS	POR-A11	1	BD29309_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A11	IGS	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	CGTAATCCTCAGTACTGGCTA	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29309_Pper_Dia2013_COI	POR-A11	1	BD29309_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	CTTAGTGGCATTAATTCGDAATCC	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.737	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29309_Sspa_Oph2007_COI	POR-A11	1	BD29309_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	GAAGGCCTTCCGATGATC	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.924	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29309_Upin_Bot2011_COI	POR-A11	1	BD29309_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Present	Sample	Upin_Bot2011_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	CTTTAATTACAGCGTTTTTATTGTTGT	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29309_Vgib_Wil2025_COI	POR-A11	1	BD29309_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	CCCCTCTTTACAAGTTCTTTAGGGT	141.61467
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29310_Cmae_Bot2011_COI	POR-A10	1	BD29310_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	ATGAACAGTCTATCCTCCTTTAG	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29310_Hsan_Wil2021_COI	POR-A10	1	BD29310_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	CCTGGGCCGGTATAGTAGGT	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.97	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29310_Mros_Gib2022_COI	POR-A10	1	BD29310_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Present	Sample	Mros_Gib2022_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29310_Msal_Bot2012_COI	POR-A10	1	BD29310_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	GYTAGTTCCRATGATGTTAGCTG	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29310_Mstr_Wil2021_COI	POR-A10	1	BD29310_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	GTAGTGGTAACTACTCATGCTTTA	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29310_Pvir_Dia2013_COI	POR-A10	1	BD29310_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	CTTAGTGGCATTAATTCGDAATCC	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29310_Rhar_Sim2018_COI	POR-A10	1	BD29310_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	CTGCTGCTATTGCTCATG	141.61328
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29312_Aamu_Bax2006_COI	POR-A08	1	BD29312_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	GCACAACCGGGATCTTTACTTC	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29312_Pcan_Bot2011_IGS	POR-A08	1	BD29312_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A08	IGS	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	CGTAATCCTCAGTACTGGCTA	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29312_Pper_Dia2013_COI	POR-A08	1	BD29312_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	CTTAGTGGCATTAATTCGDAATCC	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29312_Sspa_Oph2007_COI	POR-A08	1	BD29312_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	GAAGGCCTTCCGATGATC	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.037	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29312_Upin_Bot2011_COI	POR-A08	1	BD29312_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Present	Sample	Upin_Bot2011_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	CTTTAATTACAGCGTTTTTATTGTTGT	141.61066
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29313_Asen_Wil2025_COI	POR-A07	1	BD29313_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	GTTGTTGTTACAACACATGCTCTTTTG	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29313_Cjap_Sim2018_COI	POR-A07	1	BD29313_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29313_Esin_And2018_Cyb	POR-A07	1	BD29313_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A07	Cytb	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	ACCCCTCCTCATATCCAACCA	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29313_Mgig_Bot2012_COI	POR-A07	1	BD29313_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	TCTTATTCGTTGGAGACTTTATAACCCT	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29313_Mjap_Gib2022_COI	POR-A07	1	BD29313_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	AATTTAATTGTGACTAGGCATGGTCTG	141.62051
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29314_Cmae_Bot2011_COI	POR-A06	1	BD29314_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	ATGAACAGTCTATCCTCCTTTAG	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29314_Hsan_Wil2021_COI	POR-A06	1	BD29314_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	CCTGGGCCGGTATAGTAGGT	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29314_Mros_Gib2022_COI	POR-A06	1	BD29314_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29314_Msal_Bot2012_COI	POR-A06	1	BD29314_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	GYTAGTTCCRATGATGTTAGCTG	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29314_Mstr_Wil2021_COI	POR-A06	1	BD29314_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	GTAGTGGTAACTACTCATGCTTTA	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29314_Pvir_Dia2013_COI	POR-A06	1	BD29314_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	CTTAGTGGCATTAATTCGDAATCC	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29314_Rhar_Sim2018_COI	POR-A06	1	BD29314_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	CTGCTGCTATTGCTCATG	141.61969
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29316_Aamu_Bax2006_COI	POR-A04	1	BD29316_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	GCACAACCGGGATCTTTACTTC	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29316_Pcan_Bot2011_IGS	POR-A04	1	BD29316_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A04	IGS	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	CGTAATCCTCAGTACTGGCTA	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29316_Pper_Dia2013_COI	POR-A04	1	BD29316_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	CTTAGTGGCATTAATTCGDAATCC	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29316_Sspa_Oph2007_COI	POR-A04	1	BD29316_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	GAAGGCCTTCCGATGATC	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29316_Upin_Bot2011_COI	POR-A04	1	BD29316_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Upin_Bot2011_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	CTTTAATTACAGCGTTTTTATTGTTGT	141.61684
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29317_Cjap_Sim2018_COI	POR-A03	1	BD29317_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29317_Esin_And2018_Cyb	POR-A03	1	BD29317_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A03	Cytb	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	ACCCCTCCTCATATCCAACCA	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29317_Mgig_Bot2012_COI	POR-A03	1	BD29317_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	TCTTATTCGTTGGAGACTTTATAACCCT	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29317_Mjap_Gib2022_COI	POR-A03	1	BD29317_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	AATTTAATTGTGACTAGGCATGGTCTG	141.61122
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29318_Cmae_Bot2011_COI	POR-A02	1	BD29318_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	ATGAACAGTCTATCCTCCTTTAG	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29318_Hsan_Wil2021_COI	POR-A02	1	BD29318_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	CCTGGGCCGGTATAGTAGGT	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	33.308	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29318_Mros_Gib2022_COI	POR-A02	1	BD29318_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Present	Sample	Mros_Gib2022_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29318_Msal_Bot2012_COI	POR-A02	1	BD29318_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	GYTAGTTCCRATGATGTTAGCTG	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29318_Mstr_Wil2021_COI	POR-A02	1	BD29318_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	GTAGTGGTAACTACTCATGCTTTA	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29318_Pvir_Dia2013_COI	POR-A02	1	BD29318_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	CTTAGTGGCATTAATTCGDAATCC	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29318_Rhar_Sim2018_COI	POR-A02	1	BD29318_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	CTGCTGCTATTGCTCATG	141.62044
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29320_Cjap_Sim2018_COI	KPT-A35	1	BD29320_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29320_Esin_And2018_Cyb	KPT-A35	1	BD29320_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A35	Cytb	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	ACCCCTCCTCATATCCAACCA	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29320_Mgig_Bot2012_COI	KPT-A35	1	BD29320_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	TCTTATTCGTTGGAGACTTTATAACCCT	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29320_Mjap_Gib2022_COI	KPT-A35	1	BD29320_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	AATTTAATTGTGACTAGGCATGGTCTG	137.77845
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29321_Cmae_Bot2011_COI	KPT-A34	1	BD29321_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	ATGAACAGTCTATCCTCCTTTAG	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29321_Hsan_Wil2021_COI	KPT-A34	1	BD29321_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	CCTGGGCCGGTATAGTAGGT	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29321_Mros_Gib2022_COI	KPT-A34	1	BD29321_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29321_Msal_Bot2012_COI	KPT-A34	1	BD29321_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	GYTAGTTCCRATGATGTTAGCTG	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29321_Mstr_Wil2021_COI	KPT-A34	1	BD29321_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	GTAGTGGTAACTACTCATGCTTTA	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29321_Pvir_Dia2013_COI	KPT-A34	1	BD29321_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	CTTAGTGGCATTAATTCGDAATCC	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29321_Rhar_Sim2018_COI	KPT-A34	1	BD29321_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	CTGCTGCTATTGCTCATG	137.7771
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29323_Aamu_Bax2006_COI	KPT-A32	1	BD29323_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	GCACAACCGGGATCTTTACTTC	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29323_Pcan_Bot2011_IGS	KPT-A32	1	BD29323_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A32	IGS	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	CGTAATCCTCAGTACTGGCTA	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29323_Pper_Dia2013_COI	KPT-A32	1	BD29323_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	CTTAGTGGCATTAATTCGDAATCC	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29323_Sspa_Oph2007_COI	KPT-A32	1	BD29323_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	GAAGGCCTTCCGATGATC	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29323_Upin_Bot2011_COI	KPT-A32	1	BD29323_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	CTTTAATTACAGCGTTTTTATTGTTGT	137.77736
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29324_Cjap_Sim2018_COI	KPT-A31	1	BD29324_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29324_Esin_And2018_Cyb	KPT-A31	1	BD29324_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A31	Cytb	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	ACCCCTCCTCATATCCAACCA	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29324_Mgig_Bot2012_COI	KPT-A31	1	BD29324_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	TCTTATTCGTTGGAGACTTTATAACCCT	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29324_Mjap_Gib2022_COI	KPT-A31	1	BD29324_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	AATTTAATTGTGACTAGGCATGGTCTG	137.77984
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29325_Cmae_Bot2011_COI	KPT-A30	1	BD29325_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	ATGAACAGTCTATCCTCCTTTAG	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29325_Hsan_Wil2021_COI	KPT-A30	1	BD29325_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	CCTGGGCCGGTATAGTAGGT	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29325_Mros_Gib2022_COI	KPT-A30	1	BD29325_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29325_Msal_Bot2012_COI	KPT-A30	1	BD29325_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	GYTAGTTCCRATGATGTTAGCTG	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29325_Mstr_Wil2021_COI	KPT-A30	1	BD29325_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	GTAGTGGTAACTACTCATGCTTTA	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29325_Pvir_Dia2013_COI	KPT-A30	1	BD29325_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	CTTAGTGGCATTAATTCGDAATCC	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29325_Rhar_Sim2018_COI	KPT-A30	1	BD29325_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	CTGCTGCTATTGCTCATG	137.77845
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29327_Aamu_Bax2006_COI	KPT-A28	1	BD29327_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	GCACAACCGGGATCTTTACTTC	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29327_Pcan_Bot2011_IGS	KPT-A28	1	BD29327_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A28	IGS	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	CGTAATCCTCAGTACTGGCTA	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29327_Pper_Dia2013_COI	KPT-A28	1	BD29327_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	CTTAGTGGCATTAATTCGDAATCC	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29327_Sspa_Oph2007_COI	KPT-A28	1	BD29327_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	GAAGGCCTTCCGATGATC	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29327_Upin_Bot2011_COI	KPT-A28	1	BD29327_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	CTTTAATTACAGCGTTTTTATTGTTGT	137.77558
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29328_Cjap_Sim2018_COI	KPT-A27	1	BD29328_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29328_Esin_And2018_Cyb	KPT-A27	1	BD29328_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A27	Cytb	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	ACCCCTCCTCATATCCAACCA	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29328_Mgig_Bot2012_COI	KPT-A27	1	BD29328_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	TCTTATTCGTTGGAGACTTTATAACCCT	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29328_Mjap_Gib2022_COI	KPT-A27	1	BD29328_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	AATTTAATTGTGACTAGGCATGGTCTG	137.77676
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29329_Cmae_Bot2011_COI	KPT-A26	1	BD29329_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	ATGAACAGTCTATCCTCCTTTAG	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29329_Hsan_Wil2021_COI	KPT-A26	1	BD29329_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	CCTGGGCCGGTATAGTAGGT	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29329_Mros_Gib2022_COI	KPT-A26	1	BD29329_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29329_Msal_Bot2012_COI	KPT-A26	1	BD29329_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	GYTAGTTCCRATGATGTTAGCTG	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29329_Mstr_Wil2021_COI	KPT-A26	1	BD29329_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	GTAGTGGTAACTACTCATGCTTTA	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29329_Pvir_Dia2013_COI	KPT-A26	1	BD29329_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	CTTAGTGGCATTAATTCGDAATCC	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29329_Rhar_Sim2018_COI	KPT-A26	1	BD29329_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	CTGCTGCTATTGCTCATG	137.77811
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29330_Aamu_Bax2006_COI	KPT-A25	1	BD29330_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	GCACAACCGGGATCTTTACTTC	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29330_Pcan_Bot2011_IGS	KPT-A25	1	BD29330_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A25	IGS	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	CGTAATCCTCAGTACTGGCTA	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29330_Pper_Dia2013_COI	KPT-A25	1	BD29330_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	CTTAGTGGCATTAATTCGDAATCC	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29330_Sspa_Oph2007_COI	KPT-A25	1	BD29330_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	GAAGGCCTTCCGATGATC	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29330_Upin_Bot2011_COI	KPT-A25	1	BD29330_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	CTTTAATTACAGCGTTTTTATTGTTGT	137.77937
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29331_Cjap_Sim2018_COI	KPT-A24	1	BD29331_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29331_Esin_And2018_Cyb	KPT-A24	1	BD29331_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A24	Cytb	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	ACCCCTCCTCATATCCAACCA	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29331_Mgig_Bot2012_COI	KPT-A24	1	BD29331_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	TCTTATTCGTTGGAGACTTTATAACCCT	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29331_Mjap_Gib2022_COI	KPT-A24	1	BD29331_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	AATTTAATTGTGACTAGGCATGGTCTG	137.78109
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29332_Cmae_Bot2011_COI	KPT-A23	1	BD29332_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	ATGAACAGTCTATCCTCCTTTAG	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29332_Hsan_Wil2021_COI	KPT-A23	1	BD29332_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	CCTGGGCCGGTATAGTAGGT	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29332_Mros_Gib2022_COI	KPT-A23	1	BD29332_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29332_Msal_Bot2012_COI	KPT-A23	1	BD29332_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	GYTAGTTCCRATGATGTTAGCTG	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29332_Mstr_Wil2021_COI	KPT-A23	1	BD29332_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	GTAGTGGTAACTACTCATGCTTTA	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29332_Pvir_Dia2013_COI	KPT-A23	1	BD29332_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	CTTAGTGGCATTAATTCGDAATCC	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29332_Rhar_Sim2018_COI	KPT-A23	1	BD29332_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	CTGCTGCTATTGCTCATG	137.77976
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29334_Aamu_Bax2006_COI	KPT-A21	1	BD29334_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	GCACAACCGGGATCTTTACTTC	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29334_Pcan_Bot2011_IGS	KPT-A21	1	BD29334_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A21	IGS	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	CGTAATCCTCAGTACTGGCTA	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29334_Pper_Dia2013_COI	KPT-A21	1	BD29334_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	CTTAGTGGCATTAATTCGDAATCC	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29334_Sspa_Oph2007_COI	KPT-A21	1	BD29334_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	GAAGGCCTTCCGATGATC	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29334_Upin_Bot2011_COI	KPT-A21	1	BD29334_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	CTTTAATTACAGCGTTTTTATTGTTGT	137.77639
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29335_Cjap_Sim2018_COI	KPT-A20	1	BD29335_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29335_Esin_And2018_Cyb	KPT-A20	1	BD29335_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A20	Cytb	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	ACCCCTCCTCATATCCAACCA	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29335_Mgig_Bot2012_COI	KPT-A20	1	BD29335_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	TCTTATTCGTTGGAGACTTTATAACCCT	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29335_Mjap_Gib2022_COI	KPT-A20	1	BD29335_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	AATTTAATTGTGACTAGGCATGGTCTG	137.77572
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29336_Cmae_Bot2011_COI	KPT-A19	1	BD29336_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	ATGAACAGTCTATCCTCCTTTAG	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29336_Hsan_Wil2021_COI	KPT-A19	1	BD29336_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	CCTGGGCCGGTATAGTAGGT	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29336_Mros_Gib2022_COI	KPT-A19	1	BD29336_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29336_Msal_Bot2012_COI	KPT-A19	1	BD29336_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	GYTAGTTCCRATGATGTTAGCTG	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29336_Mstr_Wil2021_COI	KPT-A19	1	BD29336_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	GTAGTGGTAACTACTCATGCTTTA	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29336_Pvir_Dia2013_COI	KPT-A19	1	BD29336_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	CTTAGTGGCATTAATTCGDAATCC	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29336_Rhar_Sim2018_COI	KPT-A19	1	BD29336_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	CTGCTGCTATTGCTCATG	137.77707
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29338_Aamu_Bax2006_COI	KPT-A17	1	BD29338_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	GCACAACCGGGATCTTTACTTC	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29338_Pcan_Bot2011_IGS	KPT-A17	1	BD29338_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A17	IGS	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	CGTAATCCTCAGTACTGGCTA	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29338_Pper_Dia2013_COI	KPT-A17	1	BD29338_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	CTTAGTGGCATTAATTCGDAATCC	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29338_Sspa_Oph2007_COI	KPT-A17	1	BD29338_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	GAAGGCCTTCCGATGATC	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29338_Upin_Bot2011_COI	KPT-A17	1	BD29338_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	CTTTAATTACAGCGTTTTTATTGTTGT	137.77943
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29339_Cjap_Sim2018_COI	KPT-A16	1	BD29339_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29339_Esin_And2018_Cyb	KPT-A16	1	BD29339_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A16	Cytb	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	ACCCCTCCTCATATCCAACCA	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29339_Mgig_Bot2012_COI	KPT-A16	1	BD29339_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	TCTTATTCGTTGGAGACTTTATAACCCT	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29339_Mjap_Gib2022_COI	KPT-A16	1	BD29339_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	AATTTAATTGTGACTAGGCATGGTCTG	137.78114
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29341_Aamu_Bax2006_COI	KPT-A14	1	BD29341_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	GCACAACCGGGATCTTTACTTC	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29341_Pcan_Bot2011_IGS	KPT-A14	1	BD29341_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A14	IGS	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	CGTAATCCTCAGTACTGGCTA	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29341_Pper_Dia2013_COI	KPT-A14	1	BD29341_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	CTTAGTGGCATTAATTCGDAATCC	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29341_Sspa_Oph2007_COI	KPT-A14	1	BD29341_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	GAAGGCCTTCCGATGATC	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29341_Upin_Bot2011_COI	KPT-A14	1	BD29341_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	CTTTAATTACAGCGTTTTTATTGTTGT	137.7785
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29342_Cjap_Sim2018_COI	KPT-A13	1	BD29342_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29342_Esin_And2018_Cyb	KPT-A13	1	BD29342_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A13	Cytb	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	ACCCCTCCTCATATCCAACCA	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29342_Mgig_Bot2012_COI	KPT-A13	1	BD29342_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	TCTTATTCGTTGGAGACTTTATAACCCT	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29342_Mjap_Gib2022_COI	KPT-A13	1	BD29342_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	AATTTAATTGTGACTAGGCATGGTCTG	137.77723
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29343_Cmae_Bot2011_COI	KPT-A12	1	BD29343_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	ATGAACAGTCTATCCTCCTTTAG	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29343_Hsan_Wil2021_COI	KPT-A12	1	BD29343_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	CCTGGGCCGGTATAGTAGGT	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29343_Mros_Gib2022_COI	KPT-A12	1	BD29343_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29343_Msal_Bot2012_COI	KPT-A12	1	BD29343_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	GYTAGTTCCRATGATGTTAGCTG	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29343_Mstr_Wil2021_COI	KPT-A12	1	BD29343_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	GTAGTGGTAACTACTCATGCTTTA	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29343_Pvir_Dia2013_COI	KPT-A12	1	BD29343_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	CTTAGTGGCATTAATTCGDAATCC	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29343_Rhar_Sim2018_COI	KPT-A12	1	BD29343_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	CTGCTGCTATTGCTCATG	137.77589
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29345_Aamu_Bax2006_COI	KPT-A10	1	BD29345_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	GCACAACCGGGATCTTTACTTC	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29345_Pcan_Bot2011_IGS	KPT-A10	1	BD29345_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A10	IGS	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	CGTAATCCTCAGTACTGGCTA	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29345_Pper_Dia2013_COI	KPT-A10	1	BD29345_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	CTTAGTGGCATTAATTCGDAATCC	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29345_Sspa_Oph2007_COI	KPT-A10	1	BD29345_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	GAAGGCCTTCCGATGATC	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29345_Upin_Bot2011_COI	KPT-A10	1	BD29345_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	CTTTAATTACAGCGTTTTTATTGTTGT	137.77399
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29346_Cjap_Sim2018_COI	KPT-A09	1	BD29346_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29346_Esin_And2018_Cyb	KPT-A09	1	BD29346_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A09	Cytb	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	ACCCCTCCTCATATCCAACCA	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29346_Mgig_Bot2012_COI	KPT-A09	1	BD29346_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	TCTTATTCGTTGGAGACTTTATAACCCT	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29346_Mjap_Gib2022_COI	KPT-A09	1	BD29346_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	AATTTAATTGTGACTAGGCATGGTCTG	137.7745
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29347_Hsan_Wil2021_COI	KPT-A08	1	BD29347_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	CCTGGGCCGGTATAGTAGGT	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29347_Mros_Gib2022_COI	KPT-A08	1	BD29347_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29347_Msal_Bot2012_COI	KPT-A08	1	BD29347_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	GYTAGTTCCRATGATGTTAGCTG	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29347_Mstr_Wil2021_COI	KPT-A08	1	BD29347_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	GTAGTGGTAACTACTCATGCTTTA	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29347_Pvir_Dia2013_COI	KPT-A08	1	BD29347_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	CTTAGTGGCATTAATTCGDAATCC	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29347_Rhar_Sim2018_COI	KPT-A08	1	BD29347_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	CTGCTGCTATTGCTCATG	137.77573
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29349_Aamu_Bax2006_COI	KPT-A06	1	BD29349_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	GCACAACCGGGATCTTTACTTC	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29349_Pcan_Bot2011_IGS	KPT-A06	1	BD29349_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A06	IGS	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	CGTAATCCTCAGTACTGGCTA	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29349_Pper_Dia2013_COI	KPT-A06	1	BD29349_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	CTTAGTGGCATTAATTCGDAATCC	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29349_Sspa_Oph2007_COI	KPT-A06	1	BD29349_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	GAAGGCCTTCCGATGATC	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29349_Upin_Bot2011_COI	KPT-A06	1	BD29349_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	CTTTAATTACAGCGTTTTTATTGTTGT	137.77908
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29350_Cmae_Bot2011_COI	KPT-A05	1	BD29350_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	ATGAACAGTCTATCCTCCTTTAG	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29350_Hsan_Wil2021_COI	KPT-A05	1	BD29350_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	CCTGGGCCGGTATAGTAGGT	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29350_Mros_Gib2022_COI	KPT-A05	1	BD29350_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29350_Msal_Bot2012_COI	KPT-A05	1	BD29350_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	GYTAGTTCCRATGATGTTAGCTG	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29350_Mstr_Wil2021_COI	KPT-A05	1	BD29350_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	GTAGTGGTAACTACTCATGCTTTA	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29350_Pvir_Dia2013_COI	KPT-A05	1	BD29350_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	CTTAGTGGCATTAATTCGDAATCC	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29350_Rhar_Sim2018_COI	KPT-A05	1	BD29350_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	CTGCTGCTATTGCTCATG	137.78049
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29352_Aamu_Bax2006_COI	KPT-A03	1	BD29352_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	GCACAACCGGGATCTTTACTTC	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29352_Pcan_Bot2011_IGS	KPT-A03	1	BD29352_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A03	IGS	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	CGTAATCCTCAGTACTGGCTA	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29352_Pper_Dia2013_COI	KPT-A03	1	BD29352_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	CTTAGTGGCATTAATTCGDAATCC	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29352_Sspa_Oph2007_COI	KPT-A03	1	BD29352_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	GAAGGCCTTCCGATGATC	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29352_Upin_Bot2011_COI	KPT-A03	1	BD29352_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	CTTTAATTACAGCGTTTTTATTGTTGT	137.7773
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29353_Cjap_Sim2018_COI	KPT-A02	1	BD29353_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29353_Esin_And2018_Cyb	KPT-A02	1	BD29353_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A02	Cytb	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	ACCCCTCCTCATATCCAACCA	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29353_Mgig_Bot2012_COI	KPT-A02	1	BD29353_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	TCTTATTCGTTGGAGACTTTATAACCCT	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29353_Mjap_Gib2022_COI	KPT-A02	1	BD29353_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	AATTTAATTGTGACTAGGCATGGTCTG	137.77582
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29354_Cmae_Bot2011_COI	PGI-A35	1	BD29354_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	ATGAACAGTCTATCCTCCTTTAG	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29354_Hsan_Wil2021_COI	PGI-A35	1	BD29354_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	CCTGGGCCGGTATAGTAGGT	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29354_Mros_Gib2022_COI	PGI-A35	1	BD29354_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29354_Msal_Bot2012_COI	PGI-A35	1	BD29354_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	GYTAGTTCCRATGATGTTAGCTG	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29354_Mstr_Wil2021_COI	PGI-A35	1	BD29354_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	GTAGTGGTAACTACTCATGCTTTA	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29354_Pvir_Dia2013_COI	PGI-A35	1	BD29354_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	CTTAGTGGCATTAATTCGDAATCC	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29354_Rhar_Sim2018_COI	PGI-A35	1	BD29354_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	CTGCTGCTATTGCTCATG	137.76478
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29356_Aamu_Bax2006_COI	PGI-A33	1	BD29356_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	GCACAACCGGGATCTTTACTTC	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29356_Pcan_Bot2011_IGS	PGI-A33	1	BD29356_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A33	IGS	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	CGTAATCCTCAGTACTGGCTA	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29356_Pper_Dia2013_COI	PGI-A33	1	BD29356_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	CTTAGTGGCATTAATTCGDAATCC	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29356_Sspa_Oph2007_COI	PGI-A33	1	BD29356_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	GAAGGCCTTCCGATGATC	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29356_Upin_Bot2011_COI	PGI-A33	1	BD29356_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	CTTTAATTACAGCGTTTTTATTGTTGT	137.76808
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29357_Cjap_Sim2018_COI	PGI-A32	1	BD29357_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29357_Esin_And2018_Cyb	PGI-A32	1	BD29357_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A32	Cytb	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	ACCCCTCCTCATATCCAACCA	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29357_Mgig_Bot2012_COI	PGI-A32	1	BD29357_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	TCTTATTCGTTGGAGACTTTATAACCCT	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29357_Mjap_Gib2022_COI	PGI-A32	1	BD29357_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	AATTTAATTGTGACTAGGCATGGTCTG	137.76976
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29358_Cmae_Bot2011_COI	PGI-A31	1	BD29358_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	ATGAACAGTCTATCCTCCTTTAG	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29358_Hsan_Wil2021_COI	PGI-A31	1	BD29358_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	CCTGGGCCGGTATAGTAGGT	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29358_Mros_Gib2022_COI	PGI-A31	1	BD29358_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29358_Msal_Bot2012_COI	PGI-A31	1	BD29358_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	GYTAGTTCCRATGATGTTAGCTG	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29358_Mstr_Wil2021_COI	PGI-A31	1	BD29358_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	GTAGTGGTAACTACTCATGCTTTA	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29358_Pvir_Dia2013_COI	PGI-A31	1	BD29358_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	CTTAGTGGCATTAATTCGDAATCC	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29358_Rhar_Sim2018_COI	PGI-A31	1	BD29358_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	CTGCTGCTATTGCTCATG	137.77129
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29360_Cjap_Sim2018_COI	PGI-A29	1	BD29360_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29360_Esin_And2018_Cyb	PGI-A29	1	BD29360_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A29	Cytb	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	ACCCCTCCTCATATCCAACCA	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29360_Mgig_Bot2012_COI	PGI-A29	1	BD29360_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	TCTTATTCGTTGGAGACTTTATAACCCT	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29360_Mjap_Gib2022_COI	PGI-A29	1	BD29360_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	AATTTAATTGTGACTAGGCATGGTCTG	137.77066
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29361_Cmae_Bot2011_COI	PGI-A28	1	BD29361_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	ATGAACAGTCTATCCTCCTTTAG	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29361_Hsan_Wil2021_COI	PGI-A28	1	BD29361_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	CCTGGGCCGGTATAGTAGGT	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29361_Mros_Gib2022_COI	PGI-A28	1	BD29361_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29361_Msal_Bot2012_COI	PGI-A28	1	BD29361_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	GYTAGTTCCRATGATGTTAGCTG	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29361_Mstr_Wil2021_COI	PGI-A28	1	BD29361_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	GTAGTGGTAACTACTCATGCTTTA	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29361_Pvir_Dia2013_COI	PGI-A28	1	BD29361_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	CTTAGTGGCATTAATTCGDAATCC	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29361_Rhar_Sim2018_COI	PGI-A28	1	BD29361_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	CTGCTGCTATTGCTCATG	137.76915
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29363_Aamu_Bax2006_COI	PGI-A26	1	BD29363_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	GCACAACCGGGATCTTTACTTC	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29363_Pcan_Bot2011_IGS	PGI-A26	1	BD29363_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A26	IGS	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	CGTAATCCTCAGTACTGGCTA	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29363_Pper_Dia2013_COI	PGI-A26	1	BD29363_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	CTTAGTGGCATTAATTCGDAATCC	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29363_Sspa_Oph2007_COI	PGI-A26	1	BD29363_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	GAAGGCCTTCCGATGATC	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29363_Upin_Bot2011_COI	PGI-A26	1	BD29363_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	CTTTAATTACAGCGTTTTTATTGTTGT	137.76572
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29364_Cjap_Sim2018_COI	PGI-A25	1	BD29364_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29364_Esin_And2018_Cyb	PGI-A25	1	BD29364_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A25	Cytb	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	ACCCCTCCTCATATCCAACCA	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29364_Mgig_Bot2012_COI	PGI-A25	1	BD29364_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	TCTTATTCGTTGGAGACTTTATAACCCT	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29364_Mjap_Gib2022_COI	PGI-A25	1	BD29364_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	AATTTAATTGTGACTAGGCATGGTCTG	137.76465
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29365_Cmae_Bot2011_COI	PGI-A24	1	BD29365_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	ATGAACAGTCTATCCTCCTTTAG	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29365_Hsan_Wil2021_COI	PGI-A24	1	BD29365_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	CCTGGGCCGGTATAGTAGGT	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29365_Mros_Gib2022_COI	PGI-A24	1	BD29365_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29365_Msal_Bot2012_COI	PGI-A24	1	BD29365_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	GYTAGTTCCRATGATGTTAGCTG	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29365_Mstr_Wil2021_COI	PGI-A24	1	BD29365_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	GTAGTGGTAACTACTCATGCTTTA	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29365_Pvir_Dia2013_COI	PGI-A24	1	BD29365_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	CTTAGTGGCATTAATTCGDAATCC	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29365_Rhar_Sim2018_COI	PGI-A24	1	BD29365_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	CTGCTGCTATTGCTCATG	137.76629
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29367_Aamu_Bax2006_COI	PGI-A22	1	BD29367_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	GCACAACCGGGATCTTTACTTC	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29367_Pcan_Bot2011_IGS	PGI-A22	1	BD29367_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A22	IGS	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	CGTAATCCTCAGTACTGGCTA	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29367_Pper_Dia2013_COI	PGI-A22	1	BD29367_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	CTTAGTGGCATTAATTCGDAATCC	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29367_Sspa_Oph2007_COI	PGI-A22	1	BD29367_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	GAAGGCCTTCCGATGATC	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29367_Upin_Bot2011_COI	PGI-A22	1	BD29367_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	CTTTAATTACAGCGTTTTTATTGTTGT	137.76822
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29368_Cjap_Sim2018_COI	PGI-A21	1	BD29368_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29368_Esin_And2018_Cyb	PGI-A21	1	BD29368_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A21	Cytb	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	ACCCCTCCTCATATCCAACCA	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29368_Mgig_Bot2012_COI	PGI-A21	1	BD29368_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	TCTTATTCGTTGGAGACTTTATAACCCT	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29368_Mjap_Gib2022_COI	PGI-A21	1	BD29368_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	AATTTAATTGTGACTAGGCATGGTCTG	137.77254
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29369_Cmae_Bot2011_COI	PGI-A20	1	BD29369_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	ATGAACAGTCTATCCTCCTTTAG	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29369_Hsan_Wil2021_COI	PGI-A20	1	BD29369_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	CCTGGGCCGGTATAGTAGGT	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29369_Mros_Gib2022_COI	PGI-A20	1	BD29369_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29369_Msal_Bot2012_COI	PGI-A20	1	BD29369_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	GYTAGTTCCRATGATGTTAGCTG	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29369_Mstr_Wil2021_COI	PGI-A20	1	BD29369_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	GTAGTGGTAACTACTCATGCTTTA	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29369_Pvir_Dia2013_COI	PGI-A20	1	BD29369_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	CTTAGTGGCATTAATTCGDAATCC	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29369_Rhar_Sim2018_COI	PGI-A20	1	BD29369_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	CTGCTGCTATTGCTCATG	137.77337
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29370_Aamu_Bax2006_COI	PGI-A19	1	BD29370_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	GCACAACCGGGATCTTTACTTC	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29370_Pcan_Bot2011_IGS	PGI-A19	1	BD29370_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A19	IGS	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	CGTAATCCTCAGTACTGGCTA	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29370_Pper_Dia2013_COI	PGI-A19	1	BD29370_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	CTTAGTGGCATTAATTCGDAATCC	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29370_Sspa_Oph2007_COI	PGI-A19	1	BD29370_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	GAAGGCCTTCCGATGATC	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29370_Upin_Bot2011_COI	PGI-A19	1	BD29370_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	CTTTAATTACAGCGTTTTTATTGTTGT	137.77052
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29371_Cjap_Sim2018_COI	PGI-A18	1	BD29371_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29371_Esin_And2018_Cyb	PGI-A18	1	BD29371_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A18	Cytb	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	ACCCCTCCTCATATCCAACCA	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29371_Mgig_Bot2012_COI	PGI-A18	1	BD29371_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	TCTTATTCGTTGGAGACTTTATAACCCT	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29371_Mjap_Gib2022_COI	PGI-A18	1	BD29371_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	AATTTAATTGTGACTAGGCATGGTCTG	137.77238
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29372_Cmae_Bot2011_COI	PGI-A17	1	BD29372_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	ATGAACAGTCTATCCTCCTTTAG	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29372_Hsan_Wil2021_COI	PGI-A17	1	BD29372_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	CCTGGGCCGGTATAGTAGGT	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29372_Mros_Gib2022_COI	PGI-A17	1	BD29372_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29372_Msal_Bot2012_COI	PGI-A17	1	BD29372_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	GYTAGTTCCRATGATGTTAGCTG	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29372_Mstr_Wil2021_COI	PGI-A17	1	BD29372_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	GTAGTGGTAACTACTCATGCTTTA	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29372_Pvir_Dia2013_COI	PGI-A17	1	BD29372_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	CTTAGTGGCATTAATTCGDAATCC	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29372_Rhar_Sim2018_COI	PGI-A17	1	BD29372_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	CTGCTGCTATTGCTCATG	137.77262
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29374_Aamu_Bax2006_COI	PGI-A15	1	BD29374_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	GCACAACCGGGATCTTTACTTC	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29374_Pcan_Bot2011_IGS	PGI-A15	1	BD29374_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A15	IGS	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	CGTAATCCTCAGTACTGGCTA	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29374_Pper_Dia2013_COI	PGI-A15	1	BD29374_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	CTTAGTGGCATTAATTCGDAATCC	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29374_Sspa_Oph2007_COI	PGI-A15	1	BD29374_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	GAAGGCCTTCCGATGATC	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29374_Upin_Bot2011_COI	PGI-A15	1	BD29374_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	CTTTAATTACAGCGTTTTTATTGTTGT	137.7716
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29375_Cjap_Sim2018_COI	PGI-A14	1	BD29375_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29375_Esin_And2018_Cyb	PGI-A14	1	BD29375_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A14	Cytb	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	ACCCCTCCTCATATCCAACCA	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29375_Mgig_Bot2012_COI	PGI-A14	1	BD29375_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	TCTTATTCGTTGGAGACTTTATAACCCT	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29375_Mjap_Gib2022_COI	PGI-A14	1	BD29375_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	AATTTAATTGTGACTAGGCATGGTCTG	137.76969
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29376_Cmae_Bot2011_COI	PGI-A13	1	BD29376_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	ATGAACAGTCTATCCTCCTTTAG	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29376_Hsan_Wil2021_COI	PGI-A13	1	BD29376_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	CCTGGGCCGGTATAGTAGGT	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29376_Mros_Gib2022_COI	PGI-A13	1	BD29376_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29376_Msal_Bot2012_COI	PGI-A13	1	BD29376_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	GYTAGTTCCRATGATGTTAGCTG	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29376_Mstr_Wil2021_COI	PGI-A13	1	BD29376_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	GTAGTGGTAACTACTCATGCTTTA	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29376_Pvir_Dia2013_COI	PGI-A13	1	BD29376_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	CTTAGTGGCATTAATTCGDAATCC	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29376_Rhar_Sim2018_COI	PGI-A13	1	BD29376_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	CTGCTGCTATTGCTCATG	137.76788
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29378_Aamu_Bax2006_COI	PGI-A11	1	BD29378_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	GCACAACCGGGATCTTTACTTC	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29378_Pcan_Bot2011_IGS	PGI-A11	1	BD29378_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A11	IGS	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	CGTAATCCTCAGTACTGGCTA	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29378_Pper_Dia2013_COI	PGI-A11	1	BD29378_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	CTTAGTGGCATTAATTCGDAATCC	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29378_Sspa_Oph2007_COI	PGI-A11	1	BD29378_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	GAAGGCCTTCCGATGATC	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29378_Upin_Bot2011_COI	PGI-A11	1	BD29378_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	CTTTAATTACAGCGTTTTTATTGTTGT	137.76389
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29379_Cjap_Sim2018_COI	PGI-A10	1	BD29379_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29379_Esin_And2018_Cyb	PGI-A10	1	BD29379_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A10	Cytb	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	ACCCCTCCTCATATCCAACCA	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29379_Mgig_Bot2012_COI	PGI-A10	1	BD29379_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	TCTTATTCGTTGGAGACTTTATAACCCT	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29379_Mjap_Gib2022_COI	PGI-A10	1	BD29379_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	AATTTAATTGTGACTAGGCATGGTCTG	137.76517
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29381_Aamu_Bax2006_COI	PGI-A08	1	BD29381_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	GCACAACCGGGATCTTTACTTC	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29381_Pcan_Bot2011_IGS	PGI-A08	1	BD29381_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A08	IGS	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	CGTAATCCTCAGTACTGGCTA	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29381_Pper_Dia2013_COI	PGI-A08	1	BD29381_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	CTTAGTGGCATTAATTCGDAATCC	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29381_Sspa_Oph2007_COI	PGI-A08	1	BD29381_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	GAAGGCCTTCCGATGATC	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29381_Upin_Bot2011_COI	PGI-A08	1	BD29381_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	CTTTAATTACAGCGTTTTTATTGTTGT	137.76859
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29382_Cjap_Sim2018_COI	PGI-A07	1	BD29382_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29382_Esin_And2018_Cyb	PGI-A07	1	BD29382_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A07	Cytb	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	ACCCCTCCTCATATCCAACCA	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29382_Mgig_Bot2012_COI	PGI-A07	1	BD29382_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	TCTTATTCGTTGGAGACTTTATAACCCT	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29382_Mjap_Gib2022_COI	PGI-A07	1	BD29382_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	AATTTAATTGTGACTAGGCATGGTCTG	137.77035
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29383_Cmae_Bot2011_COI	PGI-A06	1	BD29383_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	ATGAACAGTCTATCCTCCTTTAG	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29383_Hsan_Wil2021_COI	PGI-A06	1	BD29383_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	CCTGGGCCGGTATAGTAGGT	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29383_Mros_Gib2022_COI	PGI-A06	1	BD29383_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29383_Msal_Bot2012_COI	PGI-A06	1	BD29383_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	GYTAGTTCCRATGATGTTAGCTG	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29383_Mstr_Wil2021_COI	PGI-A06	1	BD29383_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	GTAGTGGTAACTACTCATGCTTTA	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29383_Pvir_Dia2013_COI	PGI-A06	1	BD29383_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	CTTAGTGGCATTAATTCGDAATCC	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29383_Rhar_Sim2018_COI	PGI-A06	1	BD29383_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	CTGCTGCTATTGCTCATG	137.77222
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29385_Aamu_Bax2006_COI	PGI-A03	1	BD29385_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	GCACAACCGGGATCTTTACTTC	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29385_Pcan_Bot2011_IGS	PGI-A03	1	BD29385_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A03	IGS	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	CGTAATCCTCAGTACTGGCTA	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29385_Pper_Dia2013_COI	PGI-A03	1	BD29385_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	CTTAGTGGCATTAATTCGDAATCC	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29385_Sspa_Oph2007_COI	PGI-A03	1	BD29385_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	GAAGGCCTTCCGATGATC	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29385_Upin_Bot2011_COI	PGI-A03	1	BD29385_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	CTTTAATTACAGCGTTTTTATTGTTGT	137.76775
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29386_Cjap_Sim2018_COI	PGI-A05	1	BD29386_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29386_Esin_And2018_Cyb	PGI-A05	1	BD29386_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A05	Cytb	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	ACCCCTCCTCATATCCAACCA	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29386_Mgig_Bot2012_COI	PGI-A05	1	BD29386_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	TCTTATTCGTTGGAGACTTTATAACCCT	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29386_Mjap_Gib2022_COI	PGI-A05	1	BD29386_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	AATTTAATTGTGACTAGGCATGGTCTG	137.77132
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29387_Cmae_Bot2011_COI	PGI-A04	1	BD29387_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	ATGAACAGTCTATCCTCCTTTAG	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29387_Hsan_Wil2021_COI	PGI-A04	1	BD29387_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	CCTGGGCCGGTATAGTAGGT	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29387_Mros_Gib2022_COI	PGI-A04	1	BD29387_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29387_Msal_Bot2012_COI	PGI-A04	1	BD29387_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	GYTAGTTCCRATGATGTTAGCTG	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29387_Mstr_Wil2021_COI	PGI-A04	1	BD29387_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	GTAGTGGTAACTACTCATGCTTTA	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29387_Pvir_Dia2013_COI	PGI-A04	1	BD29387_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	CTTAGTGGCATTAATTCGDAATCC	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29387_Rhar_Sim2018_COI	PGI-A04	1	BD29387_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	CTGCTGCTATTGCTCATG	137.7696
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29389_Aamu_Bax2006_COI	KPT-A01	1	BD29389_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	GCACAACCGGGATCTTTACTTC	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29389_Pcan_Bot2011_IGS	KPT-A01	1	BD29389_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A01	IGS	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	CGTAATCCTCAGTACTGGCTA	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29389_Pper_Dia2013_COI	KPT-A01	1	BD29389_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	CTTAGTGGCATTAATTCGDAATCC	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29389_Sspa_Oph2007_COI	KPT-A01	1	BD29389_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	GAAGGCCTTCCGATGATC	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29389_Upin_Bot2011_COI	KPT-A01	1	BD29389_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	CTTTAATTACAGCGTTTTTATTGTTGT	137.7746
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42627_Pper_Dia2013_COI	ADV-01	1	BD42627_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-01	COI	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	CTTAGTGGCATTAATTCGDAATCC	144.9092464
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82108727	WGS84	BD42627	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42627_Vgib_Wil2025_COI	ADV-01	0	BD42627_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-01	COI	45	BD42627	1.00E-05	Nil	Australia	ADV-01	CCCCTCTTTACAAGTTCTTTAGGGT	144.9092464
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82164777	WGS84	BD42628	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.37	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42628_Asen_Wil2025_COI	ADV-02	0	BD42628_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Present	Sample	Asen_Wil2025_COI	ADV-02	COI	45	BD42628	1.00E-05	Nil	Australia	ADV-02	GTTGTTGTTACAACACATGCTCTTTTG	144.9096108
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42628_Cjap_Sim2018_COI	ADV-02	1	BD42628_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-02	COI	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9096108
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42628_Esin_And2018_Cyb	ADV-02	1	BD42628_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-02	Cytb	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	ACCCCTCCTCATATCCAACCA	144.9096108
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42628_Mjap_Gib2022_COI	ADV-02	1	BD42628_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-02	COI	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	AATTTAATTGTGACTAGGCATGGTCTG	144.9096108
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42629_Hsan_Wil2021_COI	ADV-03	1	BD42629_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-03	COI	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	CCTGGGCCGGTATAGTAGGT	144.9094286
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42629_Mros_Gib2022_COI	ADV-03	1	BD42629_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-03	COI	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9094286
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42629_Pvir_Dia2013_COI	ADV-03	1	BD42629_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-03	COI	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	CTTAGTGGCATTAATTCGDAATCC	144.9094286
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42629_Rhar_Sim2018_COI	ADV-03	1	BD42629_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-03	COI	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	CTGCTGCTATTGCTCATG	144.9094286
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42630_Pper_Dia2013_COI	ADV-04	1	BD42630_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-04	COI	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	CTTAGTGGCATTAATTCGDAATCC	144.9090642
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82080701	WGS84	BD42630	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42630_Vgib_Wil2025_COI	ADV-04	0	BD42630_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-04	COI	45	BD42630	1.00E-05	Nil	Australia	ADV-04	CCCCTCTTTACAAGTTCTTTAGGGT	144.9090642
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8205268	WGS84	BD42631	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.079	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42631_Asen_Wil2025_COI	ADV-05	0	BD42631_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Present	Sample	Asen_Wil2025_COI	ADV-05	COI	45	BD42631	1.00E-05	Nil	Australia	ADV-05	GTTGTTGTTACAACACATGCTCTTTTG	144.9088819
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42631_Cjap_Sim2018_COI	ADV-05	1	BD42631_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-05	COI	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9088819
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42631_Esin_And2018_Cyb	ADV-05	1	BD42631_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-05	Cytb	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	ACCCCTCCTCATATCCAACCA	144.9088819
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42631_Mjap_Gib2022_COI	ADV-05	1	BD42631_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-05	COI	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	AATTTAATTGTGACTAGGCATGGTCTG	144.9088819
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	31.451	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42632_Hsan_Wil2021_COI	ADV-06	1	BD42632_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-06	COI	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	CCTGGGCCGGTATAGTAGGT	144.8998675
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42632_Mros_Gib2022_COI	ADV-06	1	BD42632_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-06	COI	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.8998675
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42632_Pvir_Dia2013_COI	ADV-06	1	BD42632_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-06	COI	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	CTTAGTGGCATTAATTCGDAATCC	144.8998675
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42632_Rhar_Sim2018_COI	ADV-06	1	BD42632_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-06	COI	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	CTGCTGCTATTGCTCATG	144.8998675
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42634_Pper_Dia2013_COI	ADV-08	1	BD42634_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-08	COI	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	CTTAGTGGCATTAATTCGDAATCC	144.8995694
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82777317	WGS84	BD42634	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	35.179	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42634_Vgib_Wil2025_COI	ADV-08	0	BD42634_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Present	Sample	Vgib_Wil2025_COI	ADV-08	COI	45	BD42634	1.00E-05	Nil	Australia	ADV-08	CCCCTCTTTACAAGTTCTTTAGGGT	144.8995694
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82803522	WGS84	BD42635	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	35.182	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42635_Asen_Wil2025_COI	ADV-09	0	BD42635_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Present	Sample	Asen_Wil2025_COI	ADV-09	COI	45	BD42635	1.00E-05	Nil	Australia	ADV-09	GTTGTTGTTACAACACATGCTCTTTTG	144.9001655
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42635_Cjap_Sim2018_COI	ADV-09	1	BD42635_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-09	COI	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9001655
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42635_Esin_And2018_Cyb	ADV-09	1	BD42635_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-09	Cytb	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	ACCCCTCCTCATATCCAACCA	144.9001655
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42635_Mjap_Gib2022_COI	ADV-09	1	BD42635_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-09	COI	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	AATTTAATTGTGACTAGGCATGGTCTG	144.9001655
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42636_Hsan_Wil2021_COI	ADV-10	1	BD42636_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-10	COI	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	CCTGGGCCGGTATAGTAGGT	144.9215243
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42636_Mros_Gib2022_COI	ADV-10	1	BD42636_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-10	COI	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9215243
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42636_Pvir_Dia2013_COI	ADV-10	1	BD42636_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-10	COI	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	CTTAGTGGCATTAATTCGDAATCC	144.9215243
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42636_Rhar_Sim2018_COI	ADV-10	1	BD42636_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-10	COI	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	CTGCTGCTATTGCTCATG	144.9215243
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42638_Pper_Dia2013_COI	ADV-12	1	BD42638_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-12	COI	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	CTTAGTGGCATTAATTCGDAATCC	144.9287502
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84243228	WGS84	BD42638	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42638_Vgib_Wil2025_COI	ADV-12	0	BD42638_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-12	COI	45	BD42638	1.00E-05	Nil	Australia	ADV-12	CCCCTCTTTACAAGTTCTTTAGGGT	144.9287502
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84265504	WGS84	BD42639	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	35.715	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42639_Asen_Wil2025_COI	ADV-13	0	BD42639_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Present	Sample	Asen_Wil2025_COI	ADV-13	COI	45	BD42639	1.00E-05	Nil	Australia	ADV-13	GTTGTTGTTACAACACATGCTCTTTTG	144.9298511
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42639_Cjap_Sim2018_COI	ADV-13	1	BD42639_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-13	COI	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9298511
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42639_Esin_And2018_Cyb	ADV-13	1	BD42639_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-13	Cytb	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	ACCCCTCCTCATATCCAACCA	144.9298511
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42639_Mjap_Gib2022_COI	ADV-13	1	BD42639_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-13	COI	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	AATTTAATTGTGACTAGGCATGGTCTG	144.9298511
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42641_Pper_Dia2013_COI	ADV-15	1	BD42641_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-15	COI	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	CTTAGTGGCATTAATTCGDAATCC	144.9281998
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84232086	WGS84	BD42641	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42641_Vgib_Wil2025_COI	ADV-15	0	BD42641_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-15	COI	45	BD42641	1.00E-05	Nil	Australia	ADV-15	CCCCTCTTTACAAGTTCTTTAGGGT	144.9281998
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84220947	WGS84	BD42642	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.409	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42642_Asen_Wil2025_COI	ADV-16	0	BD42642_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Present	Sample	Asen_Wil2025_COI	ADV-16	COI	45	BD42642	1.00E-05	Nil	Australia	ADV-16	GTTGTTGTTACAACACATGCTCTTTTG	144.9276494
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42642_Cjap_Sim2018_COI	ADV-16	1	BD42642_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-16	COI	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9276494
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42642_Esin_And2018_Cyb	ADV-16	1	BD42642_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-16	Cytb	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	ACCCCTCCTCATATCCAACCA	144.9276494
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42642_Mjap_Gib2022_COI	ADV-16	1	BD42642_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-16	COI	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	AATTTAATTGTGACTAGGCATGGTCTG	144.9276494
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42643_Hsan_Wil2021_COI	ADV-17	1	BD42643_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-17	COI	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	CCTGGGCCGGTATAGTAGGT	144.9217039
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42643_Mros_Gib2022_COI	ADV-17	1	BD42643_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-17	COI	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9217039
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42643_Pvir_Dia2013_COI	ADV-17	1	BD42643_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-17	COI	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	CTTAGTGGCATTAATTCGDAATCC	144.9217039
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42643_Rhar_Sim2018_COI	ADV-17	1	BD42643_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-17	COI	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	CTGCTGCTATTGCTCATG	144.9217039
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42645_Pper_Dia2013_COI	ADV-19	1	BD42645_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-19	COI	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	CTTAGTGGCATTAATTCGDAATCC	144.9217938
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81895614	WGS84	BD42645	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42645_Vgib_Wil2025_COI	ADV-19	0	BD42645_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-19	COI	45	BD42645	1.00E-05	Nil	Australia	ADV-19	CCCCTCTTTACAAGTTCTTTAGGGT	144.9217938
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81921681	WGS84	BD42646	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42646_Asen_Wil2025_COI	ADV-20	0	BD42646_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-20	COI	45	BD42646	1.00E-05	Nil	Australia	ADV-20	GTTGTTGTTACAACACATGCTCTTTTG	144.9218836
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42646_Cjap_Sim2018_COI	ADV-20	1	BD42646_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-20	COI	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9218836
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42646_Esin_And2018_Cyb	ADV-20	1	BD42646_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-20	Cytb	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	ACCCCTCCTCATATCCAACCA	144.9218836
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42646_Mjap_Gib2022_COI	ADV-20	1	BD42646_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-20	COI	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	AATTTAATTGTGACTAGGCATGGTCTG	144.9218836
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42647_Hsan_Wil2021_COI	ADV-21	1	BD42647_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-21	COI	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	CCTGGGCCGGTATAGTAGGT	144.9156239
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42647_Mros_Gib2022_COI	ADV-21	1	BD42647_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-21	COI	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9156239
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42647_Pvir_Dia2013_COI	ADV-21	1	BD42647_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-21	COI	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	CTTAGTGGCATTAATTCGDAATCC	144.9156239
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42647_Rhar_Sim2018_COI	ADV-21	1	BD42647_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-21	COI	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	CTGCTGCTATTGCTCATG	144.9156239
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42649_Pper_Dia2013_COI	ADV-23	1	BD42649_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-23	COI	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	CTTAGTGGCATTAATTCGDAATCC	144.9154956
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81944634	WGS84	BD42649	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42649_Vgib_Wil2025_COI	ADV-23	0	BD42649_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-23	COI	45	BD42649	1.00E-05	Nil	Australia	ADV-23	CCCCTCTTTACAAGTTCTTTAGGGT	144.9154956
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42650_Hsan_Wil2021_COI	ADV-24	1	BD42650_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-24	COI	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	CCTGGGCCGGTATAGTAGGT	144.9157521
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42650_Mros_Gib2022_COI	ADV-24	1	BD42650_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-24	COI	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9157521
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42650_Pvir_Dia2013_COI	ADV-24	1	BD42650_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-24	COI	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	CTTAGTGGCATTAATTCGDAATCC	144.9157521
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42650_Rhar_Sim2018_COI	ADV-24	1	BD42650_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-24	COI	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	CTGCTGCTATTGCTCATG	144.9157521
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42652_Pper_Dia2013_COI	ADV-30	1	BD42652_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-30	COI	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	CTTAGTGGCATTAATTCGDAATCC	144.906424
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84537488	WGS84	BD42652	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42652_Vgib_Wil2025_COI	ADV-30	0	BD42652_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-30	COI	45	BD42652	1.00E-05	Nil	Australia	ADV-30	CCCCTCTTTACAAGTTCTTTAGGGT	144.906424
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84526043	WGS84	BD42653	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42653_Asen_Wil2025_COI	ADV-29	0	BD42653_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-29	COI	45	BD42653	1.00E-05	Nil	Australia	ADV-29	GTTGTTGTTACAACACATGCTCTTTTG	144.9067328
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42653_Cjap_Sim2018_COI	ADV-29	1	BD42653_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-29	COI	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9067328
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42653_Esin_And2018_Cyb	ADV-29	1	BD42653_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-29	Cytb	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	ACCCCTCCTCATATCCAACCA	144.9067328
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42653_Mjap_Gib2022_COI	ADV-29	1	BD42653_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-29	COI	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	AATTTAATTGTGACTAGGCATGGTCTG	144.9067328
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42654_Hsan_Wil2021_COI	ADV-28	1	BD42654_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-28	COI	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	CCTGGGCCGGTATAGTAGGT	144.9073504
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42654_Mros_Gib2022_COI	ADV-28	1	BD42654_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-28	COI	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9073504
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42654_Pvir_Dia2013_COI	ADV-28	1	BD42654_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-28	COI	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	CTTAGTGGCATTAATTCGDAATCC	144.9073504
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42654_Rhar_Sim2018_COI	ADV-28	1	BD42654_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-28	COI	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	CTGCTGCTATTGCTCATG	144.9073504
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42656_Pper_Dia2013_COI	ADV-26	1	BD42656_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-26	COI	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	CTTAGTGGCATTAATTCGDAATCC	144.9070416
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84514602	WGS84	BD42656	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42656_Vgib_Wil2025_COI	ADV-26	0	BD42656_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-26	COI	45	BD42656	1.00E-05	Nil	Australia	ADV-26	CCCCTCTTTACAAGTTCTTTAGGGT	144.9070416
BD44108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55406	WGS84	BD44108	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44108_Vgib_Wil2025_COI	ESAV-212	0	BD44108_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44108	Sample	BD44108	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-212	COI	45	BD44108	1.00E-05	Nil	Australia	ESAV-212	CCCCTCTTTACAAGTTCTTTAGGGT	147.87962
BD44109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55448	WGS84	BD44109	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44109_Asen_Wil2025_COI	ESAV-213	0	BD44109_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44109	Sample	BD44109	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-213	COI	45	BD44109	1.00E-05	Nil	Australia	ESAV-213	GTTGTTGTTACAACACATGCTCTTTTG	147.87745
BD44109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55448	WGS84	BD44109	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	31.314	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44109_Mjap_Gib2022_COI	ESAV-213	1	BD44109_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44109	Sample	BD44109	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-213	COI	45	BD44109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-213	AATTTAATTGTGACTAGGCATGGTCTG	147.87745
BD44112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55723	WGS84	BD44112	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44112_Asen_Wil2025_COI	ESAV-216	0	BD44112_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44112	Sample	BD44112	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-216	COI	45	BD44112	1.00E-05	Nil	Australia	ESAV-216	GTTGTTGTTACAACACATGCTCTTTTG	147.87394
BD44112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55723	WGS84	BD44112	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	34.55	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44112_Mjap_Gib2022_COI	ESAV-216	1	BD44112_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44112	Sample	BD44112	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-216	COI	45	BD44112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-216	AATTTAATTGTGACTAGGCATGGTCTG	147.87394
BD44113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55757	WGS84	BD44113	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.855	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44113_Mros_Gib2022_COI	ESAV-217	1	BD44113_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44113	Sample	BD44113	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-217	COI	45	BD44113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-217	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87285
BD44116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54629	WGS84	BD44116	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	33.157	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44116_Mjap_Gib2022_COI	ESAV-220	1	BD44116_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44116	Sample	BD44116	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-220	COI	45	BD44116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-220	AATTTAATTGTGACTAGGCATGGTCTG	147.92917
BD44117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5204	WGS84	BD44117	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	33.051	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44117_Mros_Gib2022_COI	ESAV-221	1	BD44117_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44117	Sample	BD44117	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-221	COI	45	BD44117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-221	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.91448
BD44119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15041	WGS84	BD44119	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	33.29	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44119_Vgib_Wil2025_COI	ESAV-204	0	BD44119_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44119	Sample	BD44119	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-204	COI	45	BD44119	1.00E-05	Nil	Australia	ESAV-204	CCCCTCTTTACAAGTTCTTTAGGGT	147.28248
BD44120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.14877	WGS84	BD44120	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44120_Mros_Gib2022_COI	ESAV-203	1	BD44120_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44120	Sample	BD44120	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-203	COI	45	BD44120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-203	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28186
BD44123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55084	WGS84	BD44123	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	32.227	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44123_Mjap_Gib2022_COI	ESAV-225	1	BD44123_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44123	Sample	BD44123	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-225	COI	45	BD44123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-225	AATTTAATTGTGACTAGGCATGGTCTG	147.90735
BD44124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51226	WGS84	BD44124	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.742	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44124_Mros_Gib2022_COI	ESAV-224	1	BD44124_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44124	Sample	BD44124	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-224	COI	45	BD44124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-224	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.91468
BD44126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15334	WGS84	BD44126	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.913	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44126_Vgib_Wil2025_COI	ESAV-206	0	BD44126_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44126	Sample	BD44126	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-206	COI	45	BD44126	1.00E-05	Nil	Australia	ESAV-206	CCCCTCTTTACAAGTTCTTTAGGGT	147.28351
BD44127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15435	WGS84	BD44127	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44127_Asen_Wil2025_COI	ESAV-207	0	BD44127_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44127	Sample	BD44127	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-207	COI	45	BD44127	1.00E-05	Nil	Australia	ESAV-207	GTTGTTGTTACAACACATGCTCTTTTG	147.2836
BD44127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15435	WGS84	BD44127	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44127_Mjap_Gib2022_COI	ESAV-207	1	BD44127_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44127	Sample	BD44127	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-207	COI	45	BD44127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-207	AATTTAATTGTGACTAGGCATGGTCTG	147.2836
BD44128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1557	WGS84	BD44128	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.654	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44128_Mros_Gib2022_COI	ESAV-208	1	BD44128_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44128	Sample	BD44128	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-208	COI	45	BD44128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-208	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28187
BD44130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15936	WGS84	BD44130	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44130_Asen_Wil2025_COI	ESAV-210	0	BD44130_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44130	Sample	BD44130	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-210	COI	45	BD44130	1.00E-05	Nil	Australia	ESAV-210	GTTGTTGTTACAACACATGCTCTTTTG	147.27943
BD44130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15936	WGS84	BD44130	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44130_Mjap_Gib2022_COI	ESAV-210	1	BD44130_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44130	Sample	BD44130	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-210	COI	45	BD44130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-210	AATTTAATTGTGACTAGGCATGGTCTG	147.27943
BD44131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.154	WGS84	BD44131	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.071	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44131_Mros_Gib2022_COI	ESAV-191	1	BD44131_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44131	Sample	BD44131	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-191	COI	45	BD44131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-191	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28375
BD44133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15661	WGS84	BD44133	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	29.325	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44133_Vgib_Wil2025_COI	ESAV-194	0	BD44133_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44133	Sample	BD44133	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-194	COI	45	BD44133	1.00E-05	Nil	Australia	ESAV-194	CCCCTCTTTACAAGTTCTTTAGGGT	147.28693
BD44134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15551	WGS84	BD44134	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.612	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44134_Asen_Wil2025_COI	ESAV-196	0	BD44134_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44134	Sample	BD44134	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-196	COI	45	BD44134	1.00E-05	Nil	Australia	ESAV-196	GTTGTTGTTACAACACATGCTCTTTTG	147.289
BD44134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15551	WGS84	BD44134	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44134_Mjap_Gib2022_COI	ESAV-196	1	BD44134_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44134	Sample	BD44134	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-196	COI	45	BD44134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-196	AATTTAATTGTGACTAGGCATGGTCTG	147.289
BD44135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15543	WGS84	BD44135	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.49	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44135_Mros_Gib2022_COI	ESAV-197	1	BD44135_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44135	Sample	BD44135	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-197	COI	45	BD44135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-197	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.29045
BD44137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15657	WGS84	BD44137	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.539	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44137_Vgib_Wil2025_COI	ESAV-201	0	BD44137_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44137	Sample	BD44137	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-201	COI	45	BD44137	1.00E-05	Nil	Australia	ESAV-201	CCCCTCTTTACAAGTTCTTTAGGGT	147.28881
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15883	WGS84	BD44138	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44138_Asen_Wil2025_COI	ESAV-001	0	BD44138_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-001	COI	45	BD44138	1.00E-05	Nil	Australia	ESAV-001	GTTGTTGTTACAACACATGCTCTTTTG	147.24663
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44138_Cjap_Sim2018_COI	ESAV-001	1	BD44138_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-001	COI	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.24663
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44138_Esin_And2018_Cyb	ESAV-001	1	BD44138_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-001	Cytb	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	ACCCCTCCTCATATCCAACCA	147.24663
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44138_Mjap_Gib2022_COI	ESAV-001	1	BD44138_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-001	COI	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	AATTTAATTGTGACTAGGCATGGTCTG	147.24663
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44139_Hsan_Wil2021_COI	ESAV-002	1	BD44139_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-002	COI	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	CCTGGGCCGGTATAGTAGGT	147.24847
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.362	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44139_Mros_Gib2022_COI	ESAV-002	1	BD44139_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-002	COI	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.24847
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44139_Pvir_Dia2013_COI	ESAV-002	1	BD44139_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-002	COI	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	CTTAGTGGCATTAATTCGDAATCC	147.24847
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44139_Rhar_Sim2018_COI	ESAV-002	1	BD44139_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-002	COI	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	CTGCTGCTATTGCTCATG	147.24847
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44140_Pper_Dia2013_COI	ESAV-003	1	BD44140_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-003	COI	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	CTTAGTGGCATTAATTCGDAATCC	147.25183
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15882	WGS84	BD44140	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.129	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44140_Vgib_Wil2025_COI	ESAV-003	0	BD44140_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-003	COI	45	BD44140	1.00E-05	Nil	Australia	ESAV-003	CCCCTCTTTACAAGTTCTTTAGGGT	147.25183
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15967	WGS84	BD44141	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44141_Asen_Wil2025_COI	ESAV-004	0	BD44141_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-004	COI	45	BD44141	1.00E-05	Nil	Australia	ESAV-004	GTTGTTGTTACAACACATGCTCTTTTG	147.25625
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44141_Cjap_Sim2018_COI	ESAV-004	1	BD44141_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-004	COI	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.25625
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44141_Esin_And2018_Cyb	ESAV-004	1	BD44141_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-004	Cytb	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	ACCCCTCCTCATATCCAACCA	147.25625
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44141_Mjap_Gib2022_COI	ESAV-004	1	BD44141_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-004	COI	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	AATTTAATTGTGACTAGGCATGGTCTG	147.25625
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44142_Hsan_Wil2021_COI	ESAV-005	1	BD44142_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-005	COI	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	CCTGGGCCGGTATAGTAGGT	147.25847
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.063	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44142_Mros_Gib2022_COI	ESAV-005	1	BD44142_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-005	COI	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.25847
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44142_Pvir_Dia2013_COI	ESAV-005	1	BD44142_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-005	COI	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	CTTAGTGGCATTAATTCGDAATCC	147.25847
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44142_Rhar_Sim2018_COI	ESAV-005	1	BD44142_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-005	COI	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	CTGCTGCTATTGCTCATG	147.25847
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44144_Pper_Dia2013_COI	ESAV-007	1	BD44144_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-007	COI	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	CTTAGTGGCATTAATTCGDAATCC	147.25872
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.16088	WGS84	BD44144	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	33.13	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44144_Vgib_Wil2025_COI	ESAV-007	0	BD44144_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-007	COI	45	BD44144	1.00E-05	Nil	Australia	ESAV-007	CCCCTCTTTACAAGTTCTTTAGGGT	147.25872
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15707	WGS84	BD44145	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.063	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44145_Asen_Wil2025_COI	ESAV-008	0	BD44145_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-008	COI	45	BD44145	1.00E-05	Nil	Australia	ESAV-008	GTTGTTGTTACAACACATGCTCTTTTG	147.28853
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44145_Cjap_Sim2018_COI	ESAV-008	1	BD44145_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-008	COI	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.28853
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44145_Esin_And2018_Cyb	ESAV-008	1	BD44145_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-008	Cytb	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	ACCCCTCCTCATATCCAACCA	147.28853
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44145_Mjap_Gib2022_COI	ESAV-008	1	BD44145_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-008	COI	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	AATTTAATTGTGACTAGGCATGGTCTG	147.28853
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44146_Hsan_Wil2021_COI	ESAV-009	1	BD44146_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-009	COI	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	CCTGGGCCGGTATAGTAGGT	147.28775
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.822	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44146_Mros_Gib2022_COI	ESAV-009	1	BD44146_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-009	COI	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28775
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44146_Pvir_Dia2013_COI	ESAV-009	1	BD44146_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-009	COI	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	CTTAGTGGCATTAATTCGDAATCC	147.28775
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44146_Rhar_Sim2018_COI	ESAV-009	1	BD44146_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-009	COI	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	CTGCTGCTATTGCTCATG	147.28775
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44148_Pper_Dia2013_COI	ESAV-011	1	BD44148_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-011	COI	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	CTTAGTGGCATTAATTCGDAATCC	147.28832
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15335	WGS84	BD44148	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	28.953	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44148_Vgib_Wil2025_COI	ESAV-011	0	BD44148_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-011	COI	45	BD44148	1.00E-05	Nil	Australia	ESAV-011	CCCCTCTTTACAAGTTCTTTAGGGT	147.28832
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15212	WGS84	BD44149	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44149_Asen_Wil2025_COI	ESAV-012	0	BD44149_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-012	COI	45	BD44149	1.00E-05	Nil	Australia	ESAV-012	GTTGTTGTTACAACACATGCTCTTTTG	147.28903
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44149_Cjap_Sim2018_COI	ESAV-012	1	BD44149_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-012	COI	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.28903
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44149_Esin_And2018_Cyb	ESAV-012	1	BD44149_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-012	Cytb	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	ACCCCTCCTCATATCCAACCA	147.28903
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44149_Mjap_Gib2022_COI	ESAV-012	1	BD44149_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-012	COI	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	AATTTAATTGTGACTAGGCATGGTCTG	147.28903
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44151_Pper_Dia2013_COI	ESAV-014	1	BD44151_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-014	COI	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	CTTAGTGGCATTAATTCGDAATCC	147.2887
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15225	WGS84	BD44151	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.757	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44151_Vgib_Wil2025_COI	ESAV-014	0	BD44151_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-014	COI	45	BD44151	1.00E-05	Nil	Australia	ESAV-014	CCCCTCTTTACAAGTTCTTTAGGGT	147.2887
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44152_Cjap_Sim2018_COI	ESAV-015	1	BD44152_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-015	COI	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.28717
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44152_Esin_And2018_Cyb	ESAV-015	1	BD44152_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-015	Cytb	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	ACCCCTCCTCATATCCAACCA	147.28717
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44152_Mjap_Gib2022_COI	ESAV-015	1	BD44152_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-015	COI	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	AATTTAATTGTGACTAGGCATGGTCTG	147.28717
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44153_Hsan_Wil2021_COI	ESAV-016	1	BD44153_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-016	COI	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	CCTGGGCCGGTATAGTAGGT	147.88314
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.919	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44153_Mros_Gib2022_COI	ESAV-016	1	BD44153_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-016	COI	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.88314
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44153_Pvir_Dia2013_COI	ESAV-016	1	BD44153_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-016	COI	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	CTTAGTGGCATTAATTCGDAATCC	147.88314
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44153_Rhar_Sim2018_COI	ESAV-016	1	BD44153_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-016	COI	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	CTGCTGCTATTGCTCATG	147.88314
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44155_Pper_Dia2013_COI	ESAV-019	1	BD44155_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-019	COI	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	CTTAGTGGCATTAATTCGDAATCC	147.88082
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44156_Cjap_Sim2018_COI	ESAV-020	1	BD44156_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-020	COI	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87966
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44156_Esin_And2018_Cyb	ESAV-020	1	BD44156_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-020	Cytb	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	ACCCCTCCTCATATCCAACCA	147.87966
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	36.697	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44156_Mjap_Gib2022_COI	ESAV-020	1	BD44156_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-020	COI	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	AATTTAATTGTGACTAGGCATGGTCTG	147.87966
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44157_Hsan_Wil2021_COI	ESAV-022	1	BD44157_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-022	COI	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	CCTGGGCCGGTATAGTAGGT	147.8773
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.039	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44157_Mros_Gib2022_COI	ESAV-022	1	BD44157_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-022	COI	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.8773
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44157_Pvir_Dia2013_COI	ESAV-022	1	BD44157_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-022	COI	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	CTTAGTGGCATTAATTCGDAATCC	147.8773
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44157_Rhar_Sim2018_COI	ESAV-022	1	BD44157_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-022	COI	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	CTGCTGCTATTGCTCATG	147.8773
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44159_Pper_Dia2013_COI	ESAV-028	1	BD44159_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-028	COI	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	CTTAGTGGCATTAATTCGDAATCC	147.87426
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44160_Hsan_Wil2021_COI	ESAV-030	1	BD44160_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-030	COI	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	CCTGGGCCGGTATAGTAGGT	147.87273
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44160_Mros_Gib2022_COI	ESAV-030	1	BD44160_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-030	COI	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87273
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44160_Pvir_Dia2013_COI	ESAV-030	1	BD44160_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-030	COI	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	CTTAGTGGCATTAATTCGDAATCC	147.87273
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44160_Rhar_Sim2018_COI	ESAV-030	1	BD44160_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-030	COI	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	CTGCTGCTATTGCTCATG	147.87273
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44162_Pper_Dia2013_COI	ESAV-033	1	BD44162_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-033	COI	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	CTTAGTGGCATTAATTCGDAATCC	147.92926
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44163_Cjap_Sim2018_COI	ESAV-034	1	BD44163_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-034	COI	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.91372
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44163_Esin_And2018_Cyb	ESAV-034	1	BD44163_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-034	Cytb	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	ACCCCTCCTCATATCCAACCA	147.91372
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	31.615	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44163_Mjap_Gib2022_COI	ESAV-034	1	BD44163_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-034	COI	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	AATTTAATTGTGACTAGGCATGGTCTG	147.91372
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44164_Hsan_Wil2021_COI	ESAV-035	1	BD44164_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-035	COI	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	CCTGGGCCGGTATAGTAGGT	147.9142
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.434	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44164_Mros_Gib2022_COI	ESAV-035	1	BD44164_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-035	COI	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.9142
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44164_Pvir_Dia2013_COI	ESAV-035	1	BD44164_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-035	COI	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	CTTAGTGGCATTAATTCGDAATCC	147.9142
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44164_Rhar_Sim2018_COI	ESAV-035	1	BD44164_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-035	COI	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	CTGCTGCTATTGCTCATG	147.9142
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44166_Pper_Dia2013_COI	ESAV-037	1	BD44166_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-037	COI	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	CTTAGTGGCATTAATTCGDAATCC	147.9156
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44167_Cjap_Sim2018_COI	ESAV-038	1	BD44167_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-038	COI	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.91067
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44167_Esin_And2018_Cyb	ESAV-038	1	BD44167_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-038	Cytb	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	ACCCCTCCTCATATCCAACCA	147.91067
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	36.784	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44167_Mjap_Gib2022_COI	ESAV-038	1	BD44167_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-038	COI	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	AATTTAATTGTGACTAGGCATGGTCTG	147.91067
BD44168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55335	WGS84	BD44168	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44168_Mros_Gib2022_COI	ESAV-039	1	BD44168_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44168	Sample	BD44168	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-039	COI	45	BD44168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-039	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.89548
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD44708_Cmae_Bot2011_COI	POR-B23	1	BD44708_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	ATGAACAGTCTATCCTCCTTTAG	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44708_Hsan_Wil2021_COI	POR-B23	1	BD44708_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	CCTGGGCCGGTATAGTAGGT	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44708_Mros_Gib2022_COI	POR-B23	1	BD44708_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD44708_Msal_Bot2012_COI	POR-B23	1	BD44708_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	GYTAGTTCCRATGATGTTAGCTG	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD44708_Mstr_Wil2021_COI	POR-B23	1	BD44708_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	GTAGTGGTAACTACTCATGCTTTA	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44708_Pvir_Dia2013_COI	POR-B23	1	BD44708_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	CTTAGTGGCATTAATTCGDAATCC	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44708_Rhar_Sim2018_COI	POR-B23	1	BD44708_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	CTGCTGCTATTGCTCATG	141.61277
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44710_Cjap_Sim2018_COI	POR-B25	1	BD44710_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44710_Esin_And2018_Cyb	POR-B25	1	BD44710_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B25	Cytb	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	ACCCCTCCTCATATCCAACCA	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44710_Mjap_Gib2022_COI	POR-B25	1	BD44710_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	AATTTAATTGTGACTAGGCATGGTCTG	141.61558
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD44711_Cmae_Bot2011_COI	POR-B26	1	BD44711_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	ATGAACAGTCTATCCTCCTTTAG	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44711_Hsan_Wil2021_COI	POR-B26	1	BD44711_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	CCTGGGCCGGTATAGTAGGT	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44711_Mros_Gib2022_COI	POR-B26	1	BD44711_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD44711_Msal_Bot2012_COI	POR-B26	1	BD44711_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	GYTAGTTCCRATGATGTTAGCTG	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD44711_Mstr_Wil2021_COI	POR-B26	1	BD44711_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	GTAGTGGTAACTACTCATGCTTTA	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44711_Pvir_Dia2013_COI	POR-B26	1	BD44711_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	CTTAGTGGCATTAATTCGDAATCC	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44711_Rhar_Sim2018_COI	POR-B26	1	BD44711_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	CTGCTGCTATTGCTCATG	141.61693
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD44713_Aamu_Bax2006_COI	POR-B28	1	BD44713_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	GCACAACCGGGATCTTTACTTC	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD44713_Pcan_Bot2011_IGS	POR-B28	1	BD44713_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B28	IGS	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	CGTAATCCTCAGTACTGGCTA	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44713_Pper_Dia2013_COI	POR-B28	1	BD44713_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	CTTAGTGGCATTAATTCGDAATCC	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD44713_Sspa_Oph2007_COI	POR-B28	1	BD44713_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	GAAGGCCTTCCGATGATC	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.782	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD44713_Upin_Bot2011_COI	POR-B28	1	BD44713_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Present	Sample	Upin_Bot2011_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	CTTTAATTACAGCGTTTTTATTGTTGT	141.61972
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44714_Cjap_Sim2018_COI	POR-B35	1	BD44714_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44714_Esin_And2018_Cyb	POR-B35	1	BD44714_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B35	Cytb	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	ACCCCTCCTCATATCCAACCA	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD44714_Mgig_Bot2012_COI	POR-B35	1	BD44714_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	TCTTATTCGTTGGAGACTTTATAACCCT	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44714_Mjap_Gib2022_COI	POR-B35	1	BD44714_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	AATTTAATTGTGACTAGGCATGGTCTG	141.61984
BD47584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.95996	WGS84	BD47584	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47584_Esin_And2018_Cyb	KPT-A38	1	BD47584_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47584	Sample	BD47584	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A38	Cytb	45	BD47584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A38	ACCCCTCCTCATATCCAACCA	137.77597
BD47584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.95996	WGS84	BD47584	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47584_Mjap_Gib2022_COI	KPT-A38	1	BD47584_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47584	Sample	BD47584	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A38	COI	45	BD47584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A38	AATTTAATTGTGACTAGGCATGGTCTG	137.77597
BD47588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85466	WGS84	BD47588	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47588_Esin_And2018_Cyb	ESAV-099	1	BD47588_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47588	Sample	BD47588	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-099	Cytb	45	BD47588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-099	ACCCCTCCTCATATCCAACCA	138.46573
BD47588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85466	WGS84	BD47588	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47588_Mjap_Gib2022_COI	ESAV-099	1	BD47588_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47588	Sample	BD47588	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-099	COI	45	BD47588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-099	AATTTAATTGTGACTAGGCATGGTCTG	138.46573
BD47591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84307	WGS84	BD47591	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47591_Esin_And2018_Cyb	ESAV-048	1	BD47591_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47591	Sample	BD47591	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-048	Cytb	45	BD47591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-048	ACCCCTCCTCATATCCAACCA	138.46395
BD47591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84307	WGS84	BD47591	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47591_Mjap_Gib2022_COI	ESAV-048	1	BD47591_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47591	Sample	BD47591	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-048	COI	45	BD47591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-048	AATTTAATTGTGACTAGGCATGGTCTG	138.46395
BD47592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8198	WGS84	BD47592	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47592_Msal_Bot2012_COI	GC30	1	BD47592_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47592	Sample	BD47592	targeted	Absent	Sample	Msal_Bot2012_COI	GC30	COI	45	BD47592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC30	GYTAGTTCCRATGATGTTAGCTG	151.24001
BD47592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8198	WGS84	BD47592	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47592_Pvir_Dia2013_COI	GC30	1	BD47592_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47592	Sample	BD47592	targeted	Absent	Sample	Pvir_Dia2013_COI	GC30	COI	45	BD47592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC30	CTTAGTGGCATTAATTCGDAATCC	151.24001
BD47595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86929	WGS84	BD47595	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47595_Esin_And2018_Cyb	ESAV-116	1	BD47595_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47595	Sample	BD47595	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-116	Cytb	45	BD47595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-116	ACCCCTCCTCATATCCAACCA	138.46534
BD47595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86929	WGS84	BD47595	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47595_Mjap_Gib2022_COI	ESAV-116	1	BD47595_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47595	Sample	BD47595	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-116	COI	45	BD47595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-116	AATTTAATTGTGACTAGGCATGGTCTG	138.46534
BD47599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82765	WGS84	BD47599	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47599_Esin_And2018_Cyb	ESAV-065	1	BD47599_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47599	Sample	BD47599	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-065	Cytb	45	BD47599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-065	ACCCCTCCTCATATCCAACCA	138.46243
BD47599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82765	WGS84	BD47599	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47599_Mjap_Gib2022_COI	ESAV-065	1	BD47599_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47599	Sample	BD47599	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-065	COI	45	BD47599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-065	AATTTAATTGTGACTAGGCATGGTCTG	138.46243
BD47600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82069	WGS84	BD47600	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47600_Hsan_Wil2021_COI	GB100	1	BD47600_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47600	Sample	BD47600	targeted	Absent	Sample	Hsan_Wil2021_COI	GB100	COI	45	BD47600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB100	CCTGGGCCGGTATAGTAGGT	151.24147
BD47600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82069	WGS84	BD47600	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47600_Msal_Bot2012_COI	GB100	1	BD47600_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47600	Sample	BD47600	targeted	Absent	Sample	Msal_Bot2012_COI	GB100	COI	45	BD47600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB100	GYTAGTTCCRATGATGTTAGCTG	151.24147
BD47603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.85332	WGS84	BD47603	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47603_Esin_And2018_Cyb	GC7	1	BD47603_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47603	Sample	BD47603	targeted	Absent	Sample	Esin_And2018_Cyb	GC7	Cytb	45	BD47603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC7	ACCCCTCCTCATATCCAACCA	151.31846
BD47603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.85332	WGS84	BD47603	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47603_Mjap_Gib2022_COI	GC7	1	BD47603_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47603	Sample	BD47603	targeted	Absent	Sample	Mjap_Gib2022_COI	GC7	COI	45	BD47603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC7	AATTTAATTGTGACTAGGCATGGTCTG	151.31846
BD47607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85707	WGS84	BD47607	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47607_Esin_And2018_Cyb	ESAV-101	1	BD47607_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47607	Sample	BD47607	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-101	Cytb	45	BD47607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-101	ACCCCTCCTCATATCCAACCA	138.46583
BD47607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85707	WGS84	BD47607	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47607_Mjap_Gib2022_COI	ESAV-101	1	BD47607_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47607	Sample	BD47607	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-101	COI	45	BD47607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-101	AATTTAATTGTGACTAGGCATGGTCTG	138.46583
BD47610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83408	WGS84	BD47610	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47610_Esin_And2018_Cyb	ESAV-058	1	BD47610_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47610	Sample	BD47610	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-058	Cytb	45	BD47610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-058	ACCCCTCCTCATATCCAACCA	138.46243
BD47610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83408	WGS84	BD47610	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47610_Mjap_Gib2022_COI	ESAV-058	1	BD47610_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47610	Sample	BD47610	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-058	COI	45	BD47610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-058	AATTTAATTGTGACTAGGCATGGTCTG	138.46243
BD47614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-32.14554	WGS84	BD47614	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47614_Esin_And2018_Cyb	THE-A40	1	BD47614_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47614	Sample	BD47614	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A40	Cytb	45	BD47614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A40	ACCCCTCCTCATATCCAACCA	133.63971
BD47614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-32.14554	WGS84	BD47614	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47614_Mjap_Gib2022_COI	THE-A40	1	BD47614_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47614	Sample	BD47614	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A40	COI	45	BD47614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A40	AATTTAATTGTGACTAGGCATGGTCTG	133.63971
BD47617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80134	WGS84	BD47617	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47617_Pcan_Bot2011_IGS	ESAV-177	1	BD47617_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47617	Sample	BD47617	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-177	IGS	45	BD47617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-177	CGTAATCCTCAGTACTGGCTA	138.48248
BD47618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86113	WGS84	BD47618	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47618_Esin_And2018_Cyb	ESAV-125	1	BD47618_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47618	Sample	BD47618	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-125	Cytb	45	BD47618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-125	ACCCCTCCTCATATCCAACCA	138.46938
BD47618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86113	WGS84	BD47618	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47618_Mjap_Gib2022_COI	ESAV-125	1	BD47618_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47618	Sample	BD47618	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-125	COI	45	BD47618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-125	AATTTAATTGTGACTAGGCATGGTCTG	138.46938
BD47621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81487	WGS84	BD47621	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47621_Esin_And2018_Cyb	ESAV-081	1	BD47621_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47621	Sample	BD47621	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-081	Cytb	45	BD47621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-081	ACCCCTCCTCATATCCAACCA	138.46389
BD47621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81487	WGS84	BD47621	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47621_Mjap_Gib2022_COI	ESAV-081	1	BD47621_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47621	Sample	BD47621	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-081	COI	45	BD47621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-081	AATTTAATTGTGACTAGGCATGGTCTG	138.46389
BD47622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83492	WGS84	BD47622	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47622_Hsan_Wil2021_COI	GB19	1	BD47622_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47622	Sample	BD47622	targeted	Absent	Sample	Hsan_Wil2021_COI	GB19	COI	45	BD47622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB19	CCTGGGCCGGTATAGTAGGT	151.27091
BD47622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83492	WGS84	BD47622	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47622_Msal_Bot2012_COI	GB19	1	BD47622_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47622	Sample	BD47622	targeted	Absent	Sample	Msal_Bot2012_COI	GB19	COI	45	BD47622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB19	GYTAGTTCCRATGATGTTAGCTG	151.27091
BD47624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-32.14771	WGS84	BD47624	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47624_Pcan_Bot2011_IGS	THE-A37	1	BD47624_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47624	Sample	BD47624	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A37	IGS	45	BD47624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A37	CGTAATCCTCAGTACTGGCTA	133.63948
BD47624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-32.14771	WGS84	BD47624	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47624_Pper_Dia2013_COI	THE-A37	1	BD47624_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47624	Sample	BD47624	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A37	COI	45	BD47624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A37	CTTAGTGGCATTAATTCGDAATCC	133.63948
BD47624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-32.14771	WGS84	BD47624	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47624_Vgib_Wil2025_COI	THE-A37	1	BD47624_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47624	Sample	BD47624	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-A37	COI	45	BD47624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A37	CCCCTCTTTACAAGTTCTTTAGGGT	133.63948
BD47625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85025	WGS84	BD47625	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47625_Esin_And2018_Cyb	ESAV-134	1	BD47625_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47625	Sample	BD47625	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-134	Cytb	45	BD47625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-134	ACCCCTCCTCATATCCAACCA	138.4712
BD47628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81925	WGS84	BD47628	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47628_Pcan_Bot2011_IGS	GC52	1	BD47628_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47628	Sample	BD47628	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC52	IGS	45	BD47628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC52	CGTAATCCTCAGTACTGGCTA	151.23972
BD47628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81925	WGS84	BD47628	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47628_Pper_Dia2013_COI	GC52	1	BD47628_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47628	Sample	BD47628	targeted	Absent	Sample	Pper_Dia2013_COI	GC52	COI	45	BD47628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC52	CTTAGTGGCATTAATTCGDAATCC	151.23972
BD47628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81925	WGS84	BD47628	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47628_Vgib_Wil2025_COI	GC52	1	BD47628_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47628	Sample	BD47628	targeted	Absent	Sample	Vgib_Wil2025_COI	GC52	COI	45	BD47628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC52	CCCCTCTTTACAAGTTCTTTAGGGT	151.23972
BD47635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8347	WGS84	BD47635	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47635_Pcan_Bot2011_IGS	GA39	1	BD47635_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47635	Sample	BD47635	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA39	IGS	45	BD47635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA39	CGTAATCCTCAGTACTGGCTA	151.27295
BD47635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8347	WGS84	BD47635	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47635_Pper_Dia2013_COI	GA39	1	BD47635_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47635	Sample	BD47635	targeted	Absent	Sample	Pper_Dia2013_COI	GA39	COI	45	BD47635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA39	CTTAGTGGCATTAATTCGDAATCC	151.27295
BD47635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8347	WGS84	BD47635	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47635_Vgib_Wil2025_COI	GA39	1	BD47635_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47635	Sample	BD47635	targeted	Absent	Sample	Vgib_Wil2025_COI	GA39	COI	45	BD47635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA39	CCCCTCTTTACAAGTTCTTTAGGGT	151.27295
BD47639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78006	WGS84	BD47639	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47639_Pcan_Bot2011_IGS	GD75	1	BD47639_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47639	Sample	BD47639	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD75	IGS	45	BD47639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD75	CGTAATCCTCAGTACTGGCTA	151.19812
BD47639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78006	WGS84	BD47639	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47639_Pper_Dia2013_COI	GD75	1	BD47639_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47639	Sample	BD47639	targeted	Absent	Sample	Pper_Dia2013_COI	GD75	COI	45	BD47639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD75	CTTAGTGGCATTAATTCGDAATCC	151.19812
BD47639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78006	WGS84	BD47639	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47639_Vgib_Wil2025_COI	GD75	1	BD47639_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47639	Sample	BD47639	targeted	Absent	Sample	Vgib_Wil2025_COI	GD75	COI	45	BD47639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD75	CCCCTCTTTACAAGTTCTTTAGGGT	151.19812
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47642_Pcan_Bot2011_IGS	GC28	1	BD47642_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC28	IGS	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	CGTAATCCTCAGTACTGGCTA	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47642_Pper_Dia2013_COI	GC28	1	BD47642_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Pper_Dia2013_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	CTTAGTGGCATTAATTCGDAATCC	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47642_Vgib_Wil2025_COI	GC28	1	BD47642_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Vgib_Wil2025_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	CCCCTCTTTACAAGTTCTTTAGGGT	151.23943
BD47643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84129	WGS84	BD47643	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47643_Esin_And2018_Cyb	ESAV-050	1	BD47643_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47643	Sample	BD47643	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-050	Cytb	45	BD47643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-050	ACCCCTCCTCATATCCAACCA	138.46358
BD47646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.8368	WGS84	BD47646	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47646_Pcan_Bot2011_IGS	ESAV-055	1	BD47646_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47646	Sample	BD47646	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-055	IGS	45	BD47646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-055	CGTAATCCTCAGTACTGGCTA	138.46281
BD47646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.8368	WGS84	BD47646	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47646_Pper_Dia2013_COI	ESAV-055	1	BD47646_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47646	Sample	BD47646	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-055	COI	45	BD47646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-055	CTTAGTGGCATTAATTCGDAATCC	138.46281
BD47646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.8368	WGS84	BD47646	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47646_Vgib_Wil2025_COI	ESAV-055	1	BD47646_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47646	Sample	BD47646	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-055	COI	45	BD47646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-055	CCCCTCTTTACAAGTTCTTTAGGGT	138.46281
BD47647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83858	WGS84	BD47647	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47647_Esin_And2018_Cyb	ESAV-053	1	BD47647_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47647	Sample	BD47647	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-053	Cytb	45	BD47647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-053	ACCCCTCCTCATATCCAACCA	138.4633
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47648_Msal_Bot2012_COI	ESAV-046	1	BD47648_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-046	COI	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	GYTAGTTCCRATGATGTTAGCTG	138.46418
BD47653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83202	WGS84	BD47653	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47653_Pcan_Bot2011_IGS	GC38	1	BD47653_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47653	Sample	BD47653	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC38	IGS	45	BD47653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC38	CGTAATCCTCAGTACTGGCTA	151.26034
BD47653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83202	WGS84	BD47653	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47653_Pper_Dia2013_COI	GC38	1	BD47653_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47653	Sample	BD47653	targeted	Absent	Sample	Pper_Dia2013_COI	GC38	COI	45	BD47653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC38	CTTAGTGGCATTAATTCGDAATCC	151.26034
BD47653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83202	WGS84	BD47653	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47653_Vgib_Wil2025_COI	GC38	1	BD47653_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47653	Sample	BD47653	targeted	Absent	Sample	Vgib_Wil2025_COI	GC38	COI	45	BD47653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC38	CCCCTCTTTACAAGTTCTTTAGGGT	151.26034
BD47654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01847	WGS84	BD47654	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47654_Esin_And2018_Cyb	ESAV-235	1	BD47654_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47654	Sample	BD47654	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-235	Cytb	45	BD47654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-235	ACCCCTCCTCATATCCAACCA	137.7687
BD47657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80774	WGS84	BD47657	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47657_Pcan_Bot2011_IGS	ESAV-171	1	BD47657_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47657	Sample	BD47657	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-171	IGS	45	BD47657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-171	CGTAATCCTCAGTACTGGCTA	138.48428
BD47657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80774	WGS84	BD47657	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47657_Pper_Dia2013_COI	ESAV-171	1	BD47657_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47657	Sample	BD47657	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-171	COI	45	BD47657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-171	CTTAGTGGCATTAATTCGDAATCC	138.48428
BD47657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80774	WGS84	BD47657	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47657_Vgib_Wil2025_COI	ESAV-171	1	BD47657_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47657	Sample	BD47657	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-171	COI	45	BD47657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-171	CCCCTCTTTACAAGTTCTTTAGGGT	138.48428
BD47660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83589	WGS84	BD47660	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47660_Pcan_Bot2011_IGS	ESAV-056	1	BD47660_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47660	Sample	BD47660	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-056	IGS	45	BD47660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-056	CGTAATCCTCAGTACTGGCTA	138.46256
BD47660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83589	WGS84	BD47660	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47660_Pper_Dia2013_COI	ESAV-056	1	BD47660_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47660	Sample	BD47660	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-056	COI	45	BD47660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-056	CTTAGTGGCATTAATTCGDAATCC	138.46256
BD47660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83589	WGS84	BD47660	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47660_Vgib_Wil2025_COI	ESAV-056	1	BD47660_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47660	Sample	BD47660	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-056	COI	45	BD47660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-056	CCCCTCTTTACAAGTTCTTTAGGGT	138.46256
BD47661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78689	WGS84	BD47661	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47661_Esin_And2018_Cyb	GB49	1	BD47661_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47661	Sample	BD47661	targeted	Absent	Sample	Esin_And2018_Cyb	GB49	Cytb	45	BD47661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB49	ACCCCTCCTCATATCCAACCA	151.17419
BD47661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78689	WGS84	BD47661	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47661_Mjap_Gib2022_COI	GB49	1	BD47661_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47661	Sample	BD47661	targeted	Absent	Sample	Mjap_Gib2022_COI	GB49	COI	45	BD47661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB49	AATTTAATTGTGACTAGGCATGGTCTG	151.17419
BD47662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83533	WGS84	BD47662	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47662_Msal_Bot2012_COI	GB17	1	BD47662_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47662	Sample	BD47662	targeted	Absent	Sample	Msal_Bot2012_COI	GB17	COI	45	BD47662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB17	GYTAGTTCCRATGATGTTAGCTG	151.27433
BD47666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82322	WGS84	BD47666	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47666_Hsan_Wil2021_COI	ESAV-156	1	BD47666_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47666	Sample	BD47666	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-156	COI	45	BD47666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-156	CCTGGGCCGGTATAGTAGGT	138.48114
BD47666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82322	WGS84	BD47666	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47666_Msal_Bot2012_COI	ESAV-156	1	BD47666_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47666	Sample	BD47666	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-156	COI	45	BD47666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-156	GYTAGTTCCRATGATGTTAGCTG	138.48114
BD47666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82322	WGS84	BD47666	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47666_Pvir_Dia2013_COI	ESAV-156	1	BD47666_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47666	Sample	BD47666	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-156	COI	45	BD47666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-156	CTTAGTGGCATTAATTCGDAATCC	138.48114
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47671_Pcan_Bot2011_IGS	GB67	1	BD47671_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB67	IGS	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	CGTAATCCTCAGTACTGGCTA	151.24434
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47671_Vgib_Wil2025_COI	GB67	1	BD47671_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Vgib_Wil2025_COI	GB67	COI	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	CCCCTCTTTACAAGTTCTTTAGGGT	151.24434
BD47673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79208	WGS84	BD47673	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47673_Hsan_Wil2021_COI	GA46	1	BD47673_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47673	Sample	BD47673	targeted	Absent	Sample	Hsan_Wil2021_COI	GA46	COI	45	BD47673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA46	CCTGGGCCGGTATAGTAGGT	151.18109
BD47673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79208	WGS84	BD47673	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47673_Msal_Bot2012_COI	GA46	1	BD47673_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47673	Sample	BD47673	targeted	Absent	Sample	Msal_Bot2012_COI	GA46	COI	45	BD47673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA46	GYTAGTTCCRATGATGTTAGCTG	151.18109
BD47673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79208	WGS84	BD47673	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47673_Pvir_Dia2013_COI	GA46	1	BD47673_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47673	Sample	BD47673	targeted	Absent	Sample	Pvir_Dia2013_COI	GA46	COI	45	BD47673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA46	CTTAGTGGCATTAATTCGDAATCC	151.18109
BD47677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86224	WGS84	BD47677	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47677_Hsan_Wil2021_COI	ESAV-106	1	BD47677_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47677	Sample	BD47677	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-106	COI	45	BD47677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-106	CCTGGGCCGGTATAGTAGGT	138.46486
BD47677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86224	WGS84	BD47677	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47677_Msal_Bot2012_COI	ESAV-106	1	BD47677_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47677	Sample	BD47677	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-106	COI	45	BD47677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-106	GYTAGTTCCRATGATGTTAGCTG	138.46486
BD47677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86224	WGS84	BD47677	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47677_Pvir_Dia2013_COI	ESAV-106	1	BD47677_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47677	Sample	BD47677	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-106	COI	45	BD47677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-106	CTTAGTGGCATTAATTCGDAATCC	138.46486
BD47680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84594	WGS84	BD47680	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47680_Hsan_Wil2021_COI	ESAV-045	1	BD47680_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47680	Sample	BD47680	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-045	COI	45	BD47680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-045	CCTGGGCCGGTATAGTAGGT	138.46425
BD47680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84594	WGS84	BD47680	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47680_Msal_Bot2012_COI	ESAV-045	1	BD47680_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47680	Sample	BD47680	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-045	COI	45	BD47680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-045	GYTAGTTCCRATGATGTTAGCTG	138.46425
BD47680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84594	WGS84	BD47680	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47680_Pvir_Dia2013_COI	ESAV-045	1	BD47680_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47680	Sample	BD47680	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-045	COI	45	BD47680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-045	CTTAGTGGCATTAATTCGDAATCC	138.46425
BD47684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14682	WGS84	BD47684	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47684_Hsan_Wil2021_COI	THE-A39	1	BD47684_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47684	Sample	BD47684	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A39	COI	45	BD47684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A39	CCTGGGCCGGTATAGTAGGT	133.6405
BD47684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14682	WGS84	BD47684	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47684_Msal_Bot2012_COI	THE-A39	1	BD47684_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47684	Sample	BD47684	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A39	COI	45	BD47684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A39	GYTAGTTCCRATGATGTTAGCTG	133.6405
BD47684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14682	WGS84	BD47684	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47684_Pvir_Dia2013_COI	THE-A39	1	BD47684_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47684	Sample	BD47684	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A39	COI	45	BD47684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A39	CTTAGTGGCATTAATTCGDAATCC	133.6405
BD47688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.8318	WGS84	BD47688	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47688_Hsan_Wil2021_COI	ESAV-147	1	BD47688_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47688	Sample	BD47688	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-147	COI	45	BD47688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-147	CCTGGGCCGGTATAGTAGGT	138.47308
BD47688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.8318	WGS84	BD47688	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47688_Msal_Bot2012_COI	ESAV-147	1	BD47688_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47688	Sample	BD47688	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-147	COI	45	BD47688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-147	GYTAGTTCCRATGATGTTAGCTG	138.47308
BD47688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.8318	WGS84	BD47688	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47688_Pvir_Dia2013_COI	ESAV-147	1	BD47688_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47688	Sample	BD47688	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-147	COI	45	BD47688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-147	CTTAGTGGCATTAATTCGDAATCC	138.47308
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47693_Pcan_Bot2011_IGS	GB78	1	BD47693_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB78	IGS	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	CGTAATCCTCAGTACTGGCTA	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47693_Pper_Dia2013_COI	GB78	1	BD47693_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Pper_Dia2013_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	CTTAGTGGCATTAATTCGDAATCC	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47693_Vgib_Wil2025_COI	GB78	1	BD47693_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Vgib_Wil2025_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	CCCCTCTTTACAAGTTCTTTAGGGT	151.23999
BD47695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80921	WGS84	BD47695	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47695_Hsan_Wil2021_COI	ESAV-169	1	BD47695_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47695	Sample	BD47695	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-169	COI	45	BD47695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-169	CCTGGGCCGGTATAGTAGGT	138.48396
BD47695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80921	WGS84	BD47695	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47695_Msal_Bot2012_COI	ESAV-169	1	BD47695_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47695	Sample	BD47695	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-169	COI	45	BD47695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-169	GYTAGTTCCRATGATGTTAGCTG	138.48396
BD47695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80921	WGS84	BD47695	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47695_Pvir_Dia2013_COI	ESAV-169	1	BD47695_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47695	Sample	BD47695	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-169	COI	45	BD47695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-169	CTTAGTGGCATTAATTCGDAATCC	138.48396
BD47697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86348	WGS84	BD47697	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47697_Vgib_Wil2025_COI	ESAV-123	1	BD47697_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47697	Sample	BD47697	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-123	COI	45	BD47697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-123	CCCCTCTTTACAAGTTCTTTAGGGT	138.46865
BD47699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80842	WGS84	BD47699	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47699_Hsan_Wil2021_COI	ESAV-086	1	BD47699_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47699	Sample	BD47699	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-086	COI	45	BD47699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-086	CCTGGGCCGGTATAGTAGGT	138.46443
BD47699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80842	WGS84	BD47699	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47699_Msal_Bot2012_COI	ESAV-086	1	BD47699_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47699	Sample	BD47699	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-086	COI	45	BD47699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-086	GYTAGTTCCRATGATGTTAGCTG	138.46443
BD47699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80842	WGS84	BD47699	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47699_Pvir_Dia2013_COI	ESAV-086	1	BD47699_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47699	Sample	BD47699	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-086	COI	45	BD47699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-086	CTTAGTGGCATTAATTCGDAATCC	138.46443
BD47701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84218	WGS84	BD47701	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47701_Pcan_Bot2011_IGS	ESAV-049	1	BD47701_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47701	Sample	BD47701	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-049	IGS	45	BD47701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-049	CGTAATCCTCAGTACTGGCTA	138.4638
BD47701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84218	WGS84	BD47701	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47701_Vgib_Wil2025_COI	ESAV-049	1	BD47701_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47701	Sample	BD47701	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-049	COI	45	BD47701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-049	CCCCTCTTTACAAGTTCTTTAGGGT	138.4638
BD47703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.78623	WGS84	BD47703	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47703_Hsan_Wil2021_COI	GA58	1	BD47703_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47703	Sample	BD47703	targeted	Absent	Sample	Hsan_Wil2021_COI	GA58	COI	45	BD47703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA58	CCTGGGCCGGTATAGTAGGT	151.17514
BD47703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.78623	WGS84	BD47703	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47703_Msal_Bot2012_COI	GA58	1	BD47703_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47703	Sample	BD47703	targeted	Absent	Sample	Msal_Bot2012_COI	GA58	COI	45	BD47703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA58	GYTAGTTCCRATGATGTTAGCTG	151.17514
BD47703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.78623	WGS84	BD47703	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47703_Pvir_Dia2013_COI	GA58	1	BD47703_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47703	Sample	BD47703	targeted	Absent	Sample	Pvir_Dia2013_COI	GA58	COI	45	BD47703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA58	CTTAGTGGCATTAATTCGDAATCC	151.17514
BD47706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82832	WGS84	BD47706	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47706_Asen_Wil2025_COI	ESAV-150	1	BD47706_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47706	Sample	BD47706	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-150	COI	45	BD47706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-150	GTTGTTGTTACAACACATGCTCTTTTG	138.47577
BD47710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84682	WGS84	BD47710	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47710_Rhar_Sim2018_COI	ESAV-044	1	BD47710_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47710	Sample	BD47710	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-044	COI	45	BD47710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-044	CTGCTGCTATTGCTCATG	138.46405
BD47713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82125	WGS84	BD47713	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47713_Asen_Wil2025_COI	GA22	1	BD47713_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47713	Sample	BD47713	targeted	Absent	Sample	Asen_Wil2025_COI	GA22	COI	45	BD47713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA22	GTTGTTGTTACAACACATGCTCTTTTG	151.24104
BD47714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80039	WGS84	BD47714	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47714_Rhar_Sim2018_COI	ESAV-178	1	BD47714_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47714	Sample	BD47714	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-178	COI	45	BD47714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-178	CTGCTGCTATTGCTCATG	138.48192
BD47718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82857	WGS84	BD47718	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47718_Rhar_Sim2018_COI	ESAV-064	1	BD47718_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47718	Sample	BD47718	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-064	COI	45	BD47718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-064	CTGCTGCTATTGCTCATG	138.46228
BD47720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84397	WGS84	BD47720	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47720_Asen_Wil2025_COI	ESAV-047	1	BD47720_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47720	Sample	BD47720	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-047	COI	45	BD47720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-047	GTTGTTGTTACAACACATGCTCTTTTG	138.46396
BD47721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83703	WGS84	BD47721	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47721_Rhar_Sim2018_COI	GC15	1	BD47721_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47721	Sample	BD47721	targeted	Absent	Sample	Rhar_Sim2018_COI	GC15	COI	45	BD47721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC15	CTGCTGCTATTGCTCATG	151.27364
BD47724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81567	WGS84	BD47724	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47724_Asen_Wil2025_COI	ESAV-164	1	BD47724_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47724	Sample	BD47724	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-164	COI	45	BD47724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-164	GTTGTTGTTACAACACATGCTCTTTTG	138.48347
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47727_Pcan_Bot2011_IGS	GB25	1	BD47727_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB25	IGS	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	CGTAATCCTCAGTACTGGCTA	151.26186
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47727_Vgib_Wil2025_COI	GB25	1	BD47727_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Vgib_Wil2025_COI	GB25	COI	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	CCCCTCTTTACAAGTTCTTTAGGGT	151.26186
BD47728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.7899	WGS84	BD47728	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47728_Asen_Wil2025_COI	GC93	1	BD47728_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47728	Sample	BD47728	targeted	Absent	Sample	Asen_Wil2025_COI	GC93	COI	45	BD47728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC93	GTTGTTGTTACAACACATGCTCTTTTG	151.17787
BD47729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84391	WGS84	BD47729	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47729_Rhar_Sim2018_COI	ESAV-091	1	BD47729_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47729	Sample	BD47729	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-091	COI	45	BD47729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-091	CTGCTGCTATTGCTCATG	138.46741
BD47732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82044	WGS84	BD47732	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47732_Rhar_Sim2018_COI	GD13	1	BD47732_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47732	Sample	BD47732	targeted	Absent	Sample	Rhar_Sim2018_COI	GD13	COI	45	BD47732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD13	CTGCTGCTATTGCTCATG	151.24022
BD47735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83609	WGS84	BD47735	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47735_Asen_Wil2025_COI	ESAV-143	1	BD47735_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47735	Sample	BD47735	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-143	COI	45	BD47735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-143	GTTGTTGTTACAACACATGCTCTTTTG	138.4729
BD47736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.85575	WGS84	BD47736	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47736_Msal_Bot2012_COI	ESAV-130	1	BD47736_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47736	Sample	BD47736	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-130	COI	45	BD47736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-130	GYTAGTTCCRATGATGTTAGCTG	138.47129
BD47736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.85575	WGS84	BD47736	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47736_Rhar_Sim2018_COI	ESAV-130	1	BD47736_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47736	Sample	BD47736	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-130	COI	45	BD47736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-130	CTGCTGCTATTGCTCATG	138.47129
BD47738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.849	WGS84	BD47738	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47738_Pcan_Bot2011_IGS	ESAV-041	1	BD47738_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47738	Sample	BD47738	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-041	IGS	45	BD47738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-041	CGTAATCCTCAGTACTGGCTA	138.4635
BD47738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.849	WGS84	BD47738	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47738_Vgib_Wil2025_COI	ESAV-041	1	BD47738_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47738	Sample	BD47738	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-041	COI	45	BD47738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-041	CCCCTCTTTACAAGTTCTTTAGGGT	138.4635
BD47739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.8171	WGS84	BD47739	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47739_Asen_Wil2025_COI	MA100	1	BD47739_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47739	Sample	BD47739	targeted	Absent	Sample	Asen_Wil2025_COI	MA100	COI	45	BD47739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA100	GTTGTTGTTACAACACATGCTCTTTTG	151.23818
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47742_Asen_Wil2025_COI	GB62	1	BD47742_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Asen_Wil2025_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	GTTGTTGTTACAACACATGCTCTTTTG	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47742_Cjap_Sim2018_COI	GB62	1	BD47742_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Cjap_Sim2018_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47742_Esin_And2018_Cyb	GB62	1	BD47742_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Esin_And2018_Cyb	GB62	Cytb	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	ACCCCTCCTCATATCCAACCA	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47742_Mjap_Gib2022_COI	GB62	1	BD47742_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Mjap_Gib2022_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	AATTTAATTGTGACTAGGCATGGTCTG	151.178
BD47743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.85192	WGS84	BD47743	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47743_Msal_Bot2012_COI	GA34	1	BD47743_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47743	Sample	BD47743	targeted	Absent	Sample	Msal_Bot2012_COI	GA34	COI	45	BD47743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA34	GYTAGTTCCRATGATGTTAGCTG	151.30959
BD47743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.85192	WGS84	BD47743	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47743_Rhar_Sim2018_COI	GA34	1	BD47743_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47743	Sample	BD47743	targeted	Absent	Sample	Rhar_Sim2018_COI	GA34	COI	45	BD47743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA34	CTGCTGCTATTGCTCATG	151.30959
BD47747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84725	WGS84	BD47747	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47747_Pvir_Dia2013_COI	ESAV-093	1	BD47747_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47747	Sample	BD47747	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-093	COI	45	BD47747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-093	CTTAGTGGCATTAATTCGDAATCC	138.46694
BD47750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8214	WGS84	BD47750	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47750_Mros_Gib2022_COI	GB75	1	BD47750_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47750	Sample	BD47750	targeted	Absent	Sample	Mros_Gib2022_COI	GB75	COI	45	BD47750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB75	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2451
BD47750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8214	WGS84	BD47750	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47750_Pvir_Dia2013_COI	GB75	1	BD47750_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47750	Sample	BD47750	targeted	Absent	Sample	Pvir_Dia2013_COI	GB75	COI	45	BD47750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB75	CTTAGTGGCATTAATTCGDAATCC	151.2451
BD47754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.79951	WGS84	BD47754	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47754_Mros_Gib2022_COI	ESAV-179	1	BD47754_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47754	Sample	BD47754	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-179	COI	45	BD47754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-179	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48135
BD47754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.79951	WGS84	BD47754	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47754_Pvir_Dia2013_COI	ESAV-179	1	BD47754_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47754	Sample	BD47754	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-179	COI	45	BD47754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-179	CTTAGTGGCATTAATTCGDAATCC	138.48135
BD47758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.82219	WGS84	BD47758	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47758_Mros_Gib2022_COI	ESAV-071	1	BD47758_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47758	Sample	BD47758	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-071	COI	45	BD47758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-071	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46291
BD47758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.82219	WGS84	BD47758	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47758_Pvir_Dia2013_COI	ESAV-071	1	BD47758_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47758	Sample	BD47758	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-071	COI	45	BD47758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-071	CTTAGTGGCATTAATTCGDAATCC	138.46291
BD47761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.7806	WGS84	BD47761	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47761_Mros_Gib2022_COI	GC76	1	BD47761_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47761	Sample	BD47761	targeted	Absent	Sample	Mros_Gib2022_COI	GC76	COI	45	BD47761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC76	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.19817
BD47761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.7806	WGS84	BD47761	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47761_Pvir_Dia2013_COI	GC76	1	BD47761_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47761	Sample	BD47761	targeted	Absent	Sample	Pvir_Dia2013_COI	GC76	COI	45	BD47761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC76	CTTAGTGGCATTAATTCGDAATCC	151.19817
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47763_Vgib_Wil2025_COI	GB11	1	BD47763_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Vgib_Wil2025_COI	GB11	COI	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	CCCCTCTTTACAAGTTCTTTAGGGT	151.31153
BD47765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.83283	WGS84	BD47765	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47765_Mros_Gib2022_COI	ESAV-146	1	BD47765_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47765	Sample	BD47765	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-146	COI	45	BD47765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-146	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47297
BD47765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.83283	WGS84	BD47765	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47765_Pvir_Dia2013_COI	ESAV-146	1	BD47765_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47765	Sample	BD47765	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-146	COI	45	BD47765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-146	CTTAGTGGCATTAATTCGDAATCC	138.47297
BD47769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86389	WGS84	BD47769	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47769_Mros_Gib2022_COI	ESAV-108	1	BD47769_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47769	Sample	BD47769	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-108	COI	45	BD47769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-108	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46517
BD47769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86389	WGS84	BD47769	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47769_Pvir_Dia2013_COI	ESAV-108	1	BD47769_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47769	Sample	BD47769	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-108	COI	45	BD47769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-108	CTTAGTGGCATTAATTCGDAATCC	138.46517
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47770_Pper_Dia2013_COI	ESAV-042	1	BD47770_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-042	COI	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	CTTAGTGGCATTAATTCGDAATCC	138.46339
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47770_Vgib_Wil2025_COI	ESAV-042	1	BD47770_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-042	COI	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	CCCCTCTTTACAAGTTCTTTAGGGT	138.46339
BD47772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.81593	WGS84	BD47772	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47772_Mros_Gib2022_COI	GD5	1	BD47772_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47772	Sample	BD47772	targeted	Absent	Sample	Mros_Gib2022_COI	GD5	COI	45	BD47772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD5	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.23638
BD47772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.81593	WGS84	BD47772	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47772_Pvir_Dia2013_COI	GD5	1	BD47772_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47772	Sample	BD47772	targeted	Absent	Sample	Pvir_Dia2013_COI	GD5	COI	45	BD47772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD5	CTTAGTGGCATTAATTCGDAATCC	151.23638
BD47775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.81663	WGS84	BD47775	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47775_Esin_And2018_Cyb	ESAV-163	1	BD47775_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47775	Sample	BD47775	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-163	Cytb	45	BD47775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-163	ACCCCTCCTCATATCCAACCA	138.48299
BD47776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86604	WGS84	BD47776	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47776_Mros_Gib2022_COI	ESAV-120	1	BD47776_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47776	Sample	BD47776	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-120	COI	45	BD47776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-120	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46741
BD47776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86604	WGS84	BD47776	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47776_Pvir_Dia2013_COI	ESAV-120	1	BD47776_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47776	Sample	BD47776	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-120	COI	45	BD47776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-120	CTTAGTGGCATTAATTCGDAATCC	138.46741
BD47779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83588	WGS84	BD47779	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47779_Esin_And2018_Cyb	GD41	1	BD47779_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47779	Sample	BD47779	targeted	Absent	Sample	Esin_And2018_Cyb	GD41	Cytb	45	BD47779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD41	ACCCCTCCTCATATCCAACCA	151.2694
BD47783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.83077	WGS84	BD47783	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47783_Mros_Gib2022_COI	ESAV-148	1	BD47783_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47783	Sample	BD47783	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-148	COI	45	BD47783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-148	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47333
BD47783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.83077	WGS84	BD47783	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47783_Pvir_Dia2013_COI	ESAV-148	1	BD47783_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47783	Sample	BD47783	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-148	COI	45	BD47783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-148	CTTAGTGGCATTAATTCGDAATCC	138.47333
BD47786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8468	WGS84	BD47786	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47786_Cjap_Sim2018_COI	ESAV-137	1	BD47786_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47786	Sample	BD47786	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-137	COI	45	BD47786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-137	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47084
BD47793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82215	WGS84	BD47793	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47793_Cjap_Sim2018_COI	GC46	1	BD47793_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47793	Sample	BD47793	targeted	Absent	Sample	Cjap_Sim2018_COI	GC46	COI	45	BD47793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC46	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.24051
BD47794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82305	WGS84	BD47794	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47794_Mros_Gib2022_COI	GB72	1	BD47794_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47794	Sample	BD47794	targeted	Absent	Sample	Mros_Gib2022_COI	GB72	COI	45	BD47794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB72	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.24253
BD47794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82305	WGS84	BD47794	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47794_Pvir_Dia2013_COI	GB72	1	BD47794_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47794	Sample	BD47794	targeted	Absent	Sample	Pvir_Dia2013_COI	GB72	COI	45	BD47794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB72	CTTAGTGGCATTAATTCGDAATCC	151.24253
BD47797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.82552	WGS84	BD47797	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47797_Cjap_Sim2018_COI	ESAV-153	1	BD47797_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47797	Sample	BD47797	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-153	COI	45	BD47797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-153	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47834
BD47801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8593	WGS84	BD47801	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47801_Cjap_Sim2018_COI	ESAV-103	1	BD47801_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47801	Sample	BD47801	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-103	COI	45	BD47801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-103	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46538
BD47805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-32.14546	WGS84	BD47805	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47805_Cjap_Sim2018_COI	THE-B36	1	BD47805_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47805	Sample	BD47805	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B36	COI	45	BD47805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B36	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63681
BD47806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.71776	WGS84	BD47806	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47806_Pvir_Dia2013_COI	ESAV-190	1	BD47806_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47806	Sample	BD47806	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-190	COI	45	BD47806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-190	CTTAGTGGCATTAATTCGDAATCC	135.88088
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47809_Asen_Wil2025_COI	GB86	1	BD47809_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Asen_Wil2025_COI	GB86	COI	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	GTTGTTGTTACAACACATGCTCTTTTG	151.19615
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47809_Cjap_Sim2018_COI	GB86	1	BD47809_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Cjap_Sim2018_COI	GB86	COI	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.19615
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47809_Esin_And2018_Cyb	GB86	1	BD47809_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Esin_And2018_Cyb	GB86	Cytb	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	ACCCCTCCTCATATCCAACCA	151.19615
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47809_Mjap_Gib2022_COI	GB86	1	BD47809_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Mjap_Gib2022_COI	GB86	COI	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	AATTTAATTGTGACTAGGCATGGTCTG	151.19615
BD47812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80529	WGS84	BD47812	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47812_Cjap_Sim2018_COI	ESAV-173	1	BD47812_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47812	Sample	BD47812	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-173	COI	45	BD47812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-173	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48388
BD47816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.85356	WGS84	BD47816	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47816_Cjap_Sim2018_COI	ESAV-098	1	BD47816_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47816	Sample	BD47816	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-098	COI	45	BD47816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-098	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46576
BD47822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8314	WGS84	BD47822	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47822_Vgib_Wil2025_COI	GD35	1	BD47822_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47822	Sample	BD47822	targeted	Absent	Sample	Vgib_Wil2025_COI	GD35	COI	45	BD47822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD35	CCCCTCTTTACAAGTTCTTTAGGGT	151.25702
BD47823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83627	WGS84	BD47823	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47823_Asen_Wil2025_COI	GC17	1	BD47823_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47823	Sample	BD47823	targeted	Absent	Sample	Asen_Wil2025_COI	GC17	COI	45	BD47823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC17	GTTGTTGTTACAACACATGCTCTTTTG	151.2724
BD47823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83627	WGS84	BD47823	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47823_Cjap_Sim2018_COI	GC17	1	BD47823_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47823	Sample	BD47823	targeted	Absent	Sample	Cjap_Sim2018_COI	GC17	COI	45	BD47823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC17	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2724
BD47823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83627	WGS84	BD47823	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47823_Esin_And2018_Cyb	GC17	1	BD47823_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47823	Sample	BD47823	targeted	Absent	Sample	Esin_And2018_Cyb	GC17	Cytb	45	BD47823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC17	ACCCCTCCTCATATCCAACCA	151.2724
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11307_Aamu_Bax2006_COI	CNSA-001	1	BE11307_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	GCACAACCGGGATCTTTACTTC	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11307_Pcan_Bot2011_IGS	CNSA-001	1	BE11307_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-001	IGS	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	CGTAATCCTCAGTACTGGCTA	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11307_Pper_Dia2013_COI	CNSA-001	1	BE11307_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	CTTAGTGGCATTAATTCGDAATCC	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11307_Sspa_Oph2007_COI	CNSA-001	1	BE11307_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	GAAGGCCTTCCGATGATC	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11307_Upin_Bot2011_COI	CNSA-001	1	BE11307_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	CTTTAATTACAGCGTTTTTATTGTTGT	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11307_Vgib_Wil2025_COI	CNSA-001	1	BE11307_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	CCCCTCTTTACAAGTTCTTTAGGGT	145.77385
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11308_Asen_Wil2025_COI	CNSA-002	1	BE11308_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	GTTGTTGTTACAACACATGCTCTTTTG	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11308_Cjap_Sim2018_COI	CNSA-002	1	BE11308_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11308_Esin_And2018_Cyb	CNSA-002	1	BE11308_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-002	Cytb	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	ACCCCTCCTCATATCCAACCA	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11308_Mgig_Bot2012_COI	CNSA-002	1	BE11308_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	TCTTATTCGTTGGAGACTTTATAACCCT	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11308_Mjap_Gib2022_COI	CNSA-002	1	BE11308_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	AATTTAATTGTGACTAGGCATGGTCTG	145.77346
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11309_Cmae_Bot2011_COI	CNSA-003	1	BE11309_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	ATGAACAGTCTATCCTCCTTTAG	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11309_Hsan_Wil2021_COI	CNSA-003	1	BE11309_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	CCTGGGCCGGTATAGTAGGT	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11309_Mros_Gib2022_COI	CNSA-003	1	BE11309_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11309_Msal_Bot2012_COI	CNSA-003	1	BE11309_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	GYTAGTTCCRATGATGTTAGCTG	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11309_Mstr_Wil2021_COI	CNSA-003	1	BE11309_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	GTAGTGGTAACTACTCATGCTTTA	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11309_Pvir_Dia2013_COI	CNSA-003	1	BE11309_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	CTTAGTGGCATTAATTCGDAATCC	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11309_Rhar_Sim2018_COI	CNSA-003	1	BE11309_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	CTGCTGCTATTGCTCATG	145.77464
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11310_Aamu_Bax2006_COI	CNSA-004	1	BE11310_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	GCACAACCGGGATCTTTACTTC	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11310_Pcan_Bot2011_IGS	CNSA-004	1	BE11310_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-004	IGS	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	CGTAATCCTCAGTACTGGCTA	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11310_Pper_Dia2013_COI	CNSA-004	1	BE11310_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	CTTAGTGGCATTAATTCGDAATCC	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11310_Sspa_Oph2007_COI	CNSA-004	1	BE11310_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	GAAGGCCTTCCGATGATC	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11310_Upin_Bot2011_COI	CNSA-004	1	BE11310_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	CTTTAATTACAGCGTTTTTATTGTTGT	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11310_Vgib_Wil2025_COI	CNSA-004	1	BE11310_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	CCCCTCTTTACAAGTTCTTTAGGGT	145.77622
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11311_Asen_Wil2025_COI	CNSA-005	1	BE11311_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	GTTGTTGTTACAACACATGCTCTTTTG	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11311_Cjap_Sim2018_COI	CNSA-005	1	BE11311_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11311_Esin_And2018_Cyb	CNSA-005	1	BE11311_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-005	Cytb	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	ACCCCTCCTCATATCCAACCA	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11311_Mgig_Bot2012_COI	CNSA-005	1	BE11311_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	TCTTATTCGTTGGAGACTTTATAACCCT	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11311_Mjap_Gib2022_COI	CNSA-005	1	BE11311_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	AATTTAATTGTGACTAGGCATGGTCTG	145.77936
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11312_Cmae_Bot2011_COI	CNSA-006	1	BE11312_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	ATGAACAGTCTATCCTCCTTTAG	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11312_Hsan_Wil2021_COI	CNSA-006	1	BE11312_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	CCTGGGCCGGTATAGTAGGT	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11312_Mros_Gib2022_COI	CNSA-006	1	BE11312_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11312_Msal_Bot2012_COI	CNSA-006	1	BE11312_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	GYTAGTTCCRATGATGTTAGCTG	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11312_Mstr_Wil2021_COI	CNSA-006	1	BE11312_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	GTAGTGGTAACTACTCATGCTTTA	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11312_Pvir_Dia2013_COI	CNSA-006	1	BE11312_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	CTTAGTGGCATTAATTCGDAATCC	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11312_Rhar_Sim2018_COI	CNSA-006	1	BE11312_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	CTGCTGCTATTGCTCATG	145.78256
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11314_Aamu_Bax2006_COI	CNSA-008	1	BE11314_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	GCACAACCGGGATCTTTACTTC	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11314_Pcan_Bot2011_IGS	CNSA-008	1	BE11314_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-008	IGS	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	CGTAATCCTCAGTACTGGCTA	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11314_Pper_Dia2013_COI	CNSA-008	1	BE11314_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	CTTAGTGGCATTAATTCGDAATCC	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11314_Sspa_Oph2007_COI	CNSA-008	1	BE11314_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	GAAGGCCTTCCGATGATC	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11314_Upin_Bot2011_COI	CNSA-008	1	BE11314_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	CTTTAATTACAGCGTTTTTATTGTTGT	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11314_Vgib_Wil2025_COI	CNSA-008	1	BE11314_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	CCCCTCTTTACAAGTTCTTTAGGGT	145.78272
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11315_Asen_Wil2025_COI	CNSA-009	1	BE11315_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	GTTGTTGTTACAACACATGCTCTTTTG	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11315_Cjap_Sim2018_COI	CNSA-009	1	BE11315_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11315_Esin_And2018_Cyb	CNSA-009	1	BE11315_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-009	Cytb	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	ACCCCTCCTCATATCCAACCA	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11315_Mgig_Bot2012_COI	CNSA-009	1	BE11315_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	TCTTATTCGTTGGAGACTTTATAACCCT	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11315_Mjap_Gib2022_COI	CNSA-009	1	BE11315_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	AATTTAATTGTGACTAGGCATGGTCTG	145.78196
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11316_Cmae_Bot2011_COI	CNSA-010	1	BE11316_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	ATGAACAGTCTATCCTCCTTTAG	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11316_Hsan_Wil2021_COI	CNSA-010	1	BE11316_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	CCTGGGCCGGTATAGTAGGT	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11316_Mros_Gib2022_COI	CNSA-010	1	BE11316_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11316_Msal_Bot2012_COI	CNSA-010	1	BE11316_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	GYTAGTTCCRATGATGTTAGCTG	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11316_Mstr_Wil2021_COI	CNSA-010	1	BE11316_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	GTAGTGGTAACTACTCATGCTTTA	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11316_Pvir_Dia2013_COI	CNSA-010	1	BE11316_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	CTTAGTGGCATTAATTCGDAATCC	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11316_Rhar_Sim2018_COI	CNSA-010	1	BE11316_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	CTGCTGCTATTGCTCATG	145.78192
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11318_Aamu_Bax2006_COI	CNSA-012	1	BE11318_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	GCACAACCGGGATCTTTACTTC	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11318_Pcan_Bot2011_IGS	CNSA-012	1	BE11318_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-012	IGS	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	CGTAATCCTCAGTACTGGCTA	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11318_Pper_Dia2013_COI	CNSA-012	1	BE11318_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	CTTAGTGGCATTAATTCGDAATCC	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11318_Sspa_Oph2007_COI	CNSA-012	1	BE11318_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	GAAGGCCTTCCGATGATC	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11318_Upin_Bot2011_COI	CNSA-012	1	BE11318_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	CTTTAATTACAGCGTTTTTATTGTTGT	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11318_Vgib_Wil2025_COI	CNSA-012	1	BE11318_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	CCCCTCTTTACAAGTTCTTTAGGGT	145.78149
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11319_Asen_Wil2025_COI	CNSA-013	1	BE11319_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	GTTGTTGTTACAACACATGCTCTTTTG	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11319_Cjap_Sim2018_COI	CNSA-013	1	BE11319_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11319_Esin_And2018_Cyb	CNSA-013	1	BE11319_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-013	Cytb	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	ACCCCTCCTCATATCCAACCA	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11319_Mgig_Bot2012_COI	CNSA-013	1	BE11319_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	TCTTATTCGTTGGAGACTTTATAACCCT	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11319_Mjap_Gib2022_COI	CNSA-013	1	BE11319_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	AATTTAATTGTGACTAGGCATGGTCTG	145.78071
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11321_Aamu_Bax2006_COI	CNSA-015	1	BE11321_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	GCACAACCGGGATCTTTACTTC	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11321_Pcan_Bot2011_IGS	CNSA-015	1	BE11321_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-015	IGS	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	CGTAATCCTCAGTACTGGCTA	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11321_Pper_Dia2013_COI	CNSA-015	1	BE11321_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	CTTAGTGGCATTAATTCGDAATCC	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11321_Sspa_Oph2007_COI	CNSA-015	1	BE11321_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	GAAGGCCTTCCGATGATC	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11321_Upin_Bot2011_COI	CNSA-015	1	BE11321_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	CTTTAATTACAGCGTTTTTATTGTTGT	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11321_Vgib_Wil2025_COI	CNSA-015	1	BE11321_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	CCCCTCTTTACAAGTTCTTTAGGGT	145.78151
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11322_Asen_Wil2025_COI	CNSA-016	1	BE11322_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	GTTGTTGTTACAACACATGCTCTTTTG	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11322_Cjap_Sim2018_COI	CNSA-016	1	BE11322_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11322_Esin_And2018_Cyb	CNSA-016	1	BE11322_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-016	Cytb	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	ACCCCTCCTCATATCCAACCA	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11322_Mgig_Bot2012_COI	CNSA-016	1	BE11322_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	TCTTATTCGTTGGAGACTTTATAACCCT	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11322_Mjap_Gib2022_COI	CNSA-016	1	BE11322_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	AATTTAATTGTGACTAGGCATGGTCTG	145.78048
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11323_Cmae_Bot2011_COI	CNSA-017	1	BE11323_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	ATGAACAGTCTATCCTCCTTTAG	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11323_Hsan_Wil2021_COI	CNSA-017	1	BE11323_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	CCTGGGCCGGTATAGTAGGT	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11323_Mros_Gib2022_COI	CNSA-017	1	BE11323_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11323_Msal_Bot2012_COI	CNSA-017	1	BE11323_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	GYTAGTTCCRATGATGTTAGCTG	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11323_Mstr_Wil2021_COI	CNSA-017	1	BE11323_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	GTAGTGGTAACTACTCATGCTTTA	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11323_Pvir_Dia2013_COI	CNSA-017	1	BE11323_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	CTTAGTGGCATTAATTCGDAATCC	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11323_Rhar_Sim2018_COI	CNSA-017	1	BE11323_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	CTGCTGCTATTGCTCATG	145.78215
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11325_Aamu_Bax2006_COI	CNSA-019	1	BE11325_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	GCACAACCGGGATCTTTACTTC	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11325_Pcan_Bot2011_IGS	CNSA-019	1	BE11325_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-019	IGS	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	CGTAATCCTCAGTACTGGCTA	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11325_Pper_Dia2013_COI	CNSA-019	1	BE11325_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	CTTAGTGGCATTAATTCGDAATCC	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11325_Sspa_Oph2007_COI	CNSA-019	1	BE11325_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	GAAGGCCTTCCGATGATC	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11325_Upin_Bot2011_COI	CNSA-019	1	BE11325_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	CTTTAATTACAGCGTTTTTATTGTTGT	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11325_Vgib_Wil2025_COI	CNSA-019	1	BE11325_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	CCCCTCTTTACAAGTTCTTTAGGGT	145.78272
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11326_Asen_Wil2025_COI	CNSA-020	1	BE11326_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	GTTGTTGTTACAACACATGCTCTTTTG	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11326_Cjap_Sim2018_COI	CNSA-020	1	BE11326_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11326_Esin_And2018_Cyb	CNSA-020	1	BE11326_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-020	Cytb	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	ACCCCTCCTCATATCCAACCA	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11326_Mgig_Bot2012_COI	CNSA-020	1	BE11326_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	TCTTATTCGTTGGAGACTTTATAACCCT	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11326_Mjap_Gib2022_COI	CNSA-020	1	BE11326_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	AATTTAATTGTGACTAGGCATGGTCTG	145.78211
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11327_Cmae_Bot2011_COI	CNSA-021	1	BE11327_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	ATGAACAGTCTATCCTCCTTTAG	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11327_Hsan_Wil2021_COI	CNSA-021	1	BE11327_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	CCTGGGCCGGTATAGTAGGT	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11327_Mros_Gib2022_COI	CNSA-021	1	BE11327_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11327_Msal_Bot2012_COI	CNSA-021	1	BE11327_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	GYTAGTTCCRATGATGTTAGCTG	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11327_Mstr_Wil2021_COI	CNSA-021	1	BE11327_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	GTAGTGGTAACTACTCATGCTTTA	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11327_Pvir_Dia2013_COI	CNSA-021	1	BE11327_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	CTTAGTGGCATTAATTCGDAATCC	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11327_Rhar_Sim2018_COI	CNSA-021	1	BE11327_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	CTGCTGCTATTGCTCATG	145.7835
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11329_Aamu_Bax2006_COI	CNSA-023	1	BE11329_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	GCACAACCGGGATCTTTACTTC	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11329_Pcan_Bot2011_IGS	CNSA-023	1	BE11329_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-023	IGS	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	CGTAATCCTCAGTACTGGCTA	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11329_Pper_Dia2013_COI	CNSA-023	1	BE11329_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	CTTAGTGGCATTAATTCGDAATCC	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11329_Sspa_Oph2007_COI	CNSA-023	1	BE11329_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	GAAGGCCTTCCGATGATC	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11329_Upin_Bot2011_COI	CNSA-023	1	BE11329_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	CTTTAATTACAGCGTTTTTATTGTTGT	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11329_Vgib_Wil2025_COI	CNSA-023	1	BE11329_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	CCCCTCTTTACAAGTTCTTTAGGGT	145.78342
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11330_Cmae_Bot2011_COI	CNSA-024	1	BE11330_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	ATGAACAGTCTATCCTCCTTTAG	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11330_Hsan_Wil2021_COI	CNSA-024	1	BE11330_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	CCTGGGCCGGTATAGTAGGT	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11330_Mros_Gib2022_COI	CNSA-024	1	BE11330_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11330_Msal_Bot2012_COI	CNSA-024	1	BE11330_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	GYTAGTTCCRATGATGTTAGCTG	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11330_Mstr_Wil2021_COI	CNSA-024	1	BE11330_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	GTAGTGGTAACTACTCATGCTTTA	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11330_Pvir_Dia2013_COI	CNSA-024	1	BE11330_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	CTTAGTGGCATTAATTCGDAATCC	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11330_Rhar_Sim2018_COI	CNSA-024	1	BE11330_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	CTGCTGCTATTGCTCATG	145.78272
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11332_Aamu_Bax2006_COI	CNSA-026	1	BE11332_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	GCACAACCGGGATCTTTACTTC	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11332_Pcan_Bot2011_IGS	CNSA-026	1	BE11332_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-026	IGS	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	CGTAATCCTCAGTACTGGCTA	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11332_Pper_Dia2013_COI	CNSA-026	1	BE11332_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	CTTAGTGGCATTAATTCGDAATCC	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11332_Sspa_Oph2007_COI	CNSA-026	1	BE11332_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	GAAGGCCTTCCGATGATC	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11332_Upin_Bot2011_COI	CNSA-026	1	BE11332_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	CTTTAATTACAGCGTTTTTATTGTTGT	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11332_Vgib_Wil2025_COI	CNSA-026	1	BE11332_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	CCCCTCTTTACAAGTTCTTTAGGGT	145.78469
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11333_Asen_Wil2025_COI	CNSA-027	1	BE11333_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	GTTGTTGTTACAACACATGCTCTTTTG	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11333_Cjap_Sim2018_COI	CNSA-027	1	BE11333_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11333_Esin_And2018_Cyb	CNSA-027	1	BE11333_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-027	Cytb	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	ACCCCTCCTCATATCCAACCA	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11333_Mgig_Bot2012_COI	CNSA-027	1	BE11333_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	TCTTATTCGTTGGAGACTTTATAACCCT	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11333_Mjap_Gib2022_COI	CNSA-027	1	BE11333_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	AATTTAATTGTGACTAGGCATGGTCTG	145.78387
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11334_Cmae_Bot2011_COI	CNSA-028	1	BE11334_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	ATGAACAGTCTATCCTCCTTTAG	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11334_Hsan_Wil2021_COI	CNSA-028	1	BE11334_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	CCTGGGCCGGTATAGTAGGT	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11334_Mros_Gib2022_COI	CNSA-028	1	BE11334_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11334_Msal_Bot2012_COI	CNSA-028	1	BE11334_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	GYTAGTTCCRATGATGTTAGCTG	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11334_Mstr_Wil2021_COI	CNSA-028	1	BE11334_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	GTAGTGGTAACTACTCATGCTTTA	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11334_Pvir_Dia2013_COI	CNSA-028	1	BE11334_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	CTTAGTGGCATTAATTCGDAATCC	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11334_Rhar_Sim2018_COI	CNSA-028	1	BE11334_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	CTGCTGCTATTGCTCATG	145.78504
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11336_Aamu_Bax2006_COI	CNSA-030	1	BE11336_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	GCACAACCGGGATCTTTACTTC	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11336_Pcan_Bot2011_IGS	CNSA-030	1	BE11336_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-030	IGS	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	CGTAATCCTCAGTACTGGCTA	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11336_Pper_Dia2013_COI	CNSA-030	1	BE11336_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	CTTAGTGGCATTAATTCGDAATCC	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11336_Sspa_Oph2007_COI	CNSA-030	1	BE11336_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	GAAGGCCTTCCGATGATC	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11336_Upin_Bot2011_COI	CNSA-030	1	BE11336_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	CTTTAATTACAGCGTTTTTATTGTTGT	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11336_Vgib_Wil2025_COI	CNSA-030	1	BE11336_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	CCCCTCTTTACAAGTTCTTTAGGGT	145.78522
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11337_Asen_Wil2025_COI	CNSA-031	1	BE11337_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	GTTGTTGTTACAACACATGCTCTTTTG	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11337_Cjap_Sim2018_COI	CNSA-031	1	BE11337_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11337_Esin_And2018_Cyb	CNSA-031	1	BE11337_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-031	Cytb	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	ACCCCTCCTCATATCCAACCA	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11337_Mgig_Bot2012_COI	CNSA-031	1	BE11337_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	TCTTATTCGTTGGAGACTTTATAACCCT	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11337_Mjap_Gib2022_COI	CNSA-031	1	BE11337_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	AATTTAATTGTGACTAGGCATGGTCTG	145.78462
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11338_Cmae_Bot2011_COI	CNSA-032	1	BE11338_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	ATGAACAGTCTATCCTCCTTTAG	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11338_Hsan_Wil2021_COI	CNSA-032	1	BE11338_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	CCTGGGCCGGTATAGTAGGT	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11338_Mros_Gib2022_COI	CNSA-032	1	BE11338_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11338_Msal_Bot2012_COI	CNSA-032	1	BE11338_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	GYTAGTTCCRATGATGTTAGCTG	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11338_Mstr_Wil2021_COI	CNSA-032	1	BE11338_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	GTAGTGGTAACTACTCATGCTTTA	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11338_Pvir_Dia2013_COI	CNSA-032	1	BE11338_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	CTTAGTGGCATTAATTCGDAATCC	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11338_Rhar_Sim2018_COI	CNSA-032	1	BE11338_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	CTGCTGCTATTGCTCATG	145.78565
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11340_Asen_Wil2025_COI	CNSA-034	1	BE11340_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	GTTGTTGTTACAACACATGCTCTTTTG	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11340_Cjap_Sim2018_COI	CNSA-034	1	BE11340_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11340_Esin_And2018_Cyb	CNSA-034	1	BE11340_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-034	Cytb	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	ACCCCTCCTCATATCCAACCA	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11340_Mgig_Bot2012_COI	CNSA-034	1	BE11340_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	TCTTATTCGTTGGAGACTTTATAACCCT	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11340_Mjap_Gib2022_COI	CNSA-034	1	BE11340_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	AATTTAATTGTGACTAGGCATGGTCTG	145.7838
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11341_Cmae_Bot2011_COI	CNSA-035	1	BE11341_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	ATGAACAGTCTATCCTCCTTTAG	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11341_Hsan_Wil2021_COI	CNSA-035	1	BE11341_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	CCTGGGCCGGTATAGTAGGT	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11341_Mros_Gib2022_COI	CNSA-035	1	BE11341_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11341_Msal_Bot2012_COI	CNSA-035	1	BE11341_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	GYTAGTTCCRATGATGTTAGCTG	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11341_Mstr_Wil2021_COI	CNSA-035	1	BE11341_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	GTAGTGGTAACTACTCATGCTTTA	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11341_Pvir_Dia2013_COI	CNSA-035	1	BE11341_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	CTTAGTGGCATTAATTCGDAATCC	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11341_Rhar_Sim2018_COI	CNSA-035	1	BE11341_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	CTGCTGCTATTGCTCATG	145.78241
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11347_Aamu_Bax2006_COI	TSVA-002	1	BE11347_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	GCACAACCGGGATCTTTACTTC	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11347_Pcan_Bot2011_IGS	TSVA-002	1	BE11347_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-002	IGS	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	CGTAATCCTCAGTACTGGCTA	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11347_Pper_Dia2013_COI	TSVA-002	1	BE11347_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	CTTAGTGGCATTAATTCGDAATCC	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11347_Sspa_Oph2007_COI	TSVA-002	1	BE11347_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	GAAGGCCTTCCGATGATC	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11347_Upin_Bot2011_COI	TSVA-002	1	BE11347_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	CTTTAATTACAGCGTTTTTATTGTTGT	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11347_Vgib_Wil2025_COI	TSVA-002	1	BE11347_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	CCCCTCTTTACAAGTTCTTTAGGGT	146.84366
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11348_Asen_Wil2025_COI	TSVA-003	1	BE11348_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	GTTGTTGTTACAACACATGCTCTTTTG	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11348_Cjap_Sim2018_COI	TSVA-003	1	BE11348_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11348_Esin_And2018_Cyb	TSVA-003	1	BE11348_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-003	Cytb	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	ACCCCTCCTCATATCCAACCA	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11348_Mgig_Bot2012_COI	TSVA-003	1	BE11348_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	TCTTATTCGTTGGAGACTTTATAACCCT	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11348_Mjap_Gib2022_COI	TSVA-003	1	BE11348_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	AATTTAATTGTGACTAGGCATGGTCTG	146.8419
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11349_Cmae_Bot2011_COI	TSVA-004	1	BE11349_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	ATGAACAGTCTATCCTCCTTTAG	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11349_Hsan_Wil2021_COI	TSVA-004	1	BE11349_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	CCTGGGCCGGTATAGTAGGT	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11349_Mros_Gib2022_COI	TSVA-004	1	BE11349_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11349_Msal_Bot2012_COI	TSVA-004	1	BE11349_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	GYTAGTTCCRATGATGTTAGCTG	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11349_Mstr_Wil2021_COI	TSVA-004	1	BE11349_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	GTAGTGGTAACTACTCATGCTTTA	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11349_Pvir_Dia2013_COI	TSVA-004	1	BE11349_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	CTTAGTGGCATTAATTCGDAATCC	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11349_Rhar_Sim2018_COI	TSVA-004	1	BE11349_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	CTGCTGCTATTGCTCATG	146.84148
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11350_Aamu_Bax2006_COI	TSVA-005	1	BE11350_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	GCACAACCGGGATCTTTACTTC	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11350_Pcan_Bot2011_IGS	TSVA-005	1	BE11350_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-005	IGS	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	CGTAATCCTCAGTACTGGCTA	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11350_Pper_Dia2013_COI	TSVA-005	1	BE11350_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	CTTAGTGGCATTAATTCGDAATCC	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11350_Sspa_Oph2007_COI	TSVA-005	1	BE11350_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	GAAGGCCTTCCGATGATC	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11350_Upin_Bot2011_COI	TSVA-005	1	BE11350_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	CTTTAATTACAGCGTTTTTATTGTTGT	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11350_Vgib_Wil2025_COI	TSVA-005	1	BE11350_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	CCCCTCTTTACAAGTTCTTTAGGGT	146.84022
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11351_Asen_Wil2025_COI	TSVA-006	1	BE11351_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	GTTGTTGTTACAACACATGCTCTTTTG	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11351_Cjap_Sim2018_COI	TSVA-006	1	BE11351_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11351_Esin_And2018_Cyb	TSVA-006	1	BE11351_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-006	Cytb	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	ACCCCTCCTCATATCCAACCA	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11351_Mgig_Bot2012_COI	TSVA-006	1	BE11351_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	TCTTATTCGTTGGAGACTTTATAACCCT	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11351_Mjap_Gib2022_COI	TSVA-006	1	BE11351_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	AATTTAATTGTGACTAGGCATGGTCTG	146.83958
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11352_Cmae_Bot2011_COI	TSVA-007	1	BE11352_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	ATGAACAGTCTATCCTCCTTTAG	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11352_Hsan_Wil2021_COI	TSVA-007	1	BE11352_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	CCTGGGCCGGTATAGTAGGT	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11352_Mros_Gib2022_COI	TSVA-007	1	BE11352_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11352_Msal_Bot2012_COI	TSVA-007	1	BE11352_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	GYTAGTTCCRATGATGTTAGCTG	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11352_Mstr_Wil2021_COI	TSVA-007	1	BE11352_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	GTAGTGGTAACTACTCATGCTTTA	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11352_Pvir_Dia2013_COI	TSVA-007	1	BE11352_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	CTTAGTGGCATTAATTCGDAATCC	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11352_Rhar_Sim2018_COI	TSVA-007	1	BE11352_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	CTGCTGCTATTGCTCATG	146.84007
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11354_Aamu_Bax2006_COI	TSVA-009	1	BE11354_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	GCACAACCGGGATCTTTACTTC	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11354_Pcan_Bot2011_IGS	TSVA-009	1	BE11354_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-009	IGS	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	CGTAATCCTCAGTACTGGCTA	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11354_Pper_Dia2013_COI	TSVA-009	1	BE11354_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	CTTAGTGGCATTAATTCGDAATCC	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11354_Sspa_Oph2007_COI	TSVA-009	1	BE11354_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	GAAGGCCTTCCGATGATC	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11354_Upin_Bot2011_COI	TSVA-009	1	BE11354_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	CTTTAATTACAGCGTTTTTATTGTTGT	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11354_Vgib_Wil2025_COI	TSVA-009	1	BE11354_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	CCCCTCTTTACAAGTTCTTTAGGGT	146.83964
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11355_Asen_Wil2025_COI	TSVA-010	1	BE11355_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	GTTGTTGTTACAACACATGCTCTTTTG	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11355_Cjap_Sim2018_COI	TSVA-010	1	BE11355_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11355_Esin_And2018_Cyb	TSVA-010	1	BE11355_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-010	Cytb	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	ACCCCTCCTCATATCCAACCA	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11355_Mgig_Bot2012_COI	TSVA-010	1	BE11355_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	TCTTATTCGTTGGAGACTTTATAACCCT	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11355_Mjap_Gib2022_COI	TSVA-010	1	BE11355_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	AATTTAATTGTGACTAGGCATGGTCTG	146.84064
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11356_Cmae_Bot2011_COI	TSVA-011	1	BE11356_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	ATGAACAGTCTATCCTCCTTTAG	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11356_Hsan_Wil2021_COI	TSVA-011	1	BE11356_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	CCTGGGCCGGTATAGTAGGT	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11356_Mros_Gib2022_COI	TSVA-011	1	BE11356_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11356_Msal_Bot2012_COI	TSVA-011	1	BE11356_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	GYTAGTTCCRATGATGTTAGCTG	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11356_Mstr_Wil2021_COI	TSVA-011	1	BE11356_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	GTAGTGGTAACTACTCATGCTTTA	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11356_Pvir_Dia2013_COI	TSVA-011	1	BE11356_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	CTTAGTGGCATTAATTCGDAATCC	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11356_Rhar_Sim2018_COI	TSVA-011	1	BE11356_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	CTGCTGCTATTGCTCATG	146.84188
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11358_Aamu_Bax2006_COI	TSVA-013	1	BE11358_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	GCACAACCGGGATCTTTACTTC	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11358_Pcan_Bot2011_IGS	TSVA-013	1	BE11358_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-013	IGS	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	CGTAATCCTCAGTACTGGCTA	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11358_Pper_Dia2013_COI	TSVA-013	1	BE11358_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	CTTAGTGGCATTAATTCGDAATCC	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11358_Sspa_Oph2007_COI	TSVA-013	1	BE11358_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	GAAGGCCTTCCGATGATC	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11358_Upin_Bot2011_COI	TSVA-013	1	BE11358_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	CTTTAATTACAGCGTTTTTATTGTTGT	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11358_Vgib_Wil2025_COI	TSVA-013	1	BE11358_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	CCCCTCTTTACAAGTTCTTTAGGGT	146.83785
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11359_Asen_Wil2025_COI	TSVA-014	1	BE11359_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	GTTGTTGTTACAACACATGCTCTTTTG	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11359_Cjap_Sim2018_COI	TSVA-014	1	BE11359_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11359_Esin_And2018_Cyb	TSVA-014	1	BE11359_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-014	Cytb	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	ACCCCTCCTCATATCCAACCA	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11359_Mgig_Bot2012_COI	TSVA-014	1	BE11359_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	TCTTATTCGTTGGAGACTTTATAACCCT	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11359_Mjap_Gib2022_COI	TSVA-014	1	BE11359_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	AATTTAATTGTGACTAGGCATGGTCTG	146.83647
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11361_Aamu_Bax2006_COI	TSVA-016	1	BE11361_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	GCACAACCGGGATCTTTACTTC	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11361_Pcan_Bot2011_IGS	TSVA-016	1	BE11361_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-016	IGS	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	CGTAATCCTCAGTACTGGCTA	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11361_Pper_Dia2013_COI	TSVA-016	1	BE11361_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	CTTAGTGGCATTAATTCGDAATCC	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11361_Sspa_Oph2007_COI	TSVA-016	1	BE11361_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	GAAGGCCTTCCGATGATC	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11361_Upin_Bot2011_COI	TSVA-016	1	BE11361_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	CTTTAATTACAGCGTTTTTATTGTTGT	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11361_Vgib_Wil2025_COI	TSVA-016	1	BE11361_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	CCCCTCTTTACAAGTTCTTTAGGGT	146.83612
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11362_Asen_Wil2025_COI	TSVA-017	1	BE11362_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	GTTGTTGTTACAACACATGCTCTTTTG	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11362_Cjap_Sim2018_COI	TSVA-017	1	BE11362_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11362_Esin_And2018_Cyb	TSVA-017	1	BE11362_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-017	Cytb	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	ACCCCTCCTCATATCCAACCA	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11362_Mgig_Bot2012_COI	TSVA-017	1	BE11362_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	TCTTATTCGTTGGAGACTTTATAACCCT	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11362_Mjap_Gib2022_COI	TSVA-017	1	BE11362_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	AATTTAATTGTGACTAGGCATGGTCTG	146.83385
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11363_Cmae_Bot2011_COI	TSVA-018	1	BE11363_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	ATGAACAGTCTATCCTCCTTTAG	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11363_Hsan_Wil2021_COI	TSVA-018	1	BE11363_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	CCTGGGCCGGTATAGTAGGT	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11363_Mros_Gib2022_COI	TSVA-018	1	BE11363_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11363_Msal_Bot2012_COI	TSVA-018	1	BE11363_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	GYTAGTTCCRATGATGTTAGCTG	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11363_Mstr_Wil2021_COI	TSVA-018	1	BE11363_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	GTAGTGGTAACTACTCATGCTTTA	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11363_Pvir_Dia2013_COI	TSVA-018	1	BE11363_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	CTTAGTGGCATTAATTCGDAATCC	146.83475
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11365_Aamu_Bax2006_COI	TSVA-020	1	BE11365_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	GCACAACCGGGATCTTTACTTC	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11365_Pcan_Bot2011_IGS	TSVA-020	1	BE11365_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-020	IGS	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	CGTAATCCTCAGTACTGGCTA	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11365_Pper_Dia2013_COI	TSVA-020	1	BE11365_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	CTTAGTGGCATTAATTCGDAATCC	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11365_Sspa_Oph2007_COI	TSVA-020	1	BE11365_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	GAAGGCCTTCCGATGATC	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11365_Upin_Bot2011_COI	TSVA-020	1	BE11365_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	CTTTAATTACAGCGTTTTTATTGTTGT	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11365_Vgib_Wil2025_COI	TSVA-020	1	BE11365_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	CCCCTCTTTACAAGTTCTTTAGGGT	146.83638
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11366_Asen_Wil2025_COI	TSVA-021	1	BE11366_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	GTTGTTGTTACAACACATGCTCTTTTG	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11366_Cjap_Sim2018_COI	TSVA-021	1	BE11366_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11366_Esin_And2018_Cyb	TSVA-021	1	BE11366_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-021	Cytb	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	ACCCCTCCTCATATCCAACCA	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11366_Mgig_Bot2012_COI	TSVA-021	1	BE11366_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	TCTTATTCGTTGGAGACTTTATAACCCT	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11366_Mjap_Gib2022_COI	TSVA-021	1	BE11366_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	AATTTAATTGTGACTAGGCATGGTCTG	146.83773
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11367_Cmae_Bot2011_COI	TSVA-022	1	BE11367_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	ATGAACAGTCTATCCTCCTTTAG	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11367_Hsan_Wil2021_COI	TSVA-022	1	BE11367_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	CCTGGGCCGGTATAGTAGGT	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11367_Mros_Gib2022_COI	TSVA-022	1	BE11367_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11367_Msal_Bot2012_COI	TSVA-022	1	BE11367_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	GYTAGTTCCRATGATGTTAGCTG	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11367_Mstr_Wil2021_COI	TSVA-022	1	BE11367_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	GTAGTGGTAACTACTCATGCTTTA	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11367_Pvir_Dia2013_COI	TSVA-022	1	BE11367_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	CTTAGTGGCATTAATTCGDAATCC	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11367_Rhar_Sim2018_COI	TSVA-022	1	BE11367_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	CTGCTGCTATTGCTCATG	146.83784
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11369_Aamu_Bax2006_COI	TSVA-024	1	BE11369_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	GCACAACCGGGATCTTTACTTC	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11369_Pcan_Bot2011_IGS	TSVA-024	1	BE11369_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-024	IGS	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	CGTAATCCTCAGTACTGGCTA	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11369_Pper_Dia2013_COI	TSVA-024	1	BE11369_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	CTTAGTGGCATTAATTCGDAATCC	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11369_Sspa_Oph2007_COI	TSVA-024	1	BE11369_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	GAAGGCCTTCCGATGATC	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11369_Upin_Bot2011_COI	TSVA-024	1	BE11369_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	CTTTAATTACAGCGTTTTTATTGTTGT	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11369_Vgib_Wil2025_COI	TSVA-024	1	BE11369_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	CCCCTCTTTACAAGTTCTTTAGGGT	146.835
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11370_Cmae_Bot2011_COI	TSVA-025	1	BE11370_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	ATGAACAGTCTATCCTCCTTTAG	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11370_Hsan_Wil2021_COI	TSVA-025	1	BE11370_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	CCTGGGCCGGTATAGTAGGT	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11370_Mros_Gib2022_COI	TSVA-025	1	BE11370_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11370_Msal_Bot2012_COI	TSVA-025	1	BE11370_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	GYTAGTTCCRATGATGTTAGCTG	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11370_Mstr_Wil2021_COI	TSVA-025	1	BE11370_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	GTAGTGGTAACTACTCATGCTTTA	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11370_Pvir_Dia2013_COI	TSVA-025	1	BE11370_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	CTTAGTGGCATTAATTCGDAATCC	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11370_Rhar_Sim2018_COI	TSVA-025	1	BE11370_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	CTGCTGCTATTGCTCATG	146.83313
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11372_Aamu_Bax2006_COI	TSVA-027	1	BE11372_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	GCACAACCGGGATCTTTACTTC	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11372_Pcan_Bot2011_IGS	TSVA-027	1	BE11372_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-027	IGS	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	CGTAATCCTCAGTACTGGCTA	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11372_Pper_Dia2013_COI	TSVA-027	1	BE11372_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	CTTAGTGGCATTAATTCGDAATCC	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11372_Sspa_Oph2007_COI	TSVA-027	1	BE11372_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	GAAGGCCTTCCGATGATC	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11372_Upin_Bot2011_COI	TSVA-027	1	BE11372_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	CTTTAATTACAGCGTTTTTATTGTTGT	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11372_Vgib_Wil2025_COI	TSVA-027	1	BE11372_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	CCCCTCTTTACAAGTTCTTTAGGGT	146.83145
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11373_Asen_Wil2025_COI	TSVA-028	1	BE11373_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	GTTGTTGTTACAACACATGCTCTTTTG	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11373_Cjap_Sim2018_COI	TSVA-028	1	BE11373_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11373_Esin_And2018_Cyb	TSVA-028	1	BE11373_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-028	Cytb	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	ACCCCTCCTCATATCCAACCA	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11373_Mgig_Bot2012_COI	TSVA-028	1	BE11373_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	TCTTATTCGTTGGAGACTTTATAACCCT	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11373_Mjap_Gib2022_COI	TSVA-028	1	BE11373_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	AATTTAATTGTGACTAGGCATGGTCTG	146.83453
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11374_Cmae_Bot2011_COI	TSVA-029	1	BE11374_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	ATGAACAGTCTATCCTCCTTTAG	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11374_Hsan_Wil2021_COI	TSVA-029	1	BE11374_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	CCTGGGCCGGTATAGTAGGT	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11374_Mros_Gib2022_COI	TSVA-029	1	BE11374_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11374_Msal_Bot2012_COI	TSVA-029	1	BE11374_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	GYTAGTTCCRATGATGTTAGCTG	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11374_Mstr_Wil2021_COI	TSVA-029	1	BE11374_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	GTAGTGGTAACTACTCATGCTTTA	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11374_Pvir_Dia2013_COI	TSVA-029	1	BE11374_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	CTTAGTGGCATTAATTCGDAATCC	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11374_Rhar_Sim2018_COI	TSVA-029	1	BE11374_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	CTGCTGCTATTGCTCATG	146.83552
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11376_Aamu_Bax2006_COI	TSVA-031	1	BE11376_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	GCACAACCGGGATCTTTACTTC	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11376_Pcan_Bot2011_IGS	TSVA-031	1	BE11376_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-031	IGS	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	CGTAATCCTCAGTACTGGCTA	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11376_Pper_Dia2013_COI	TSVA-031	1	BE11376_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	CTTAGTGGCATTAATTCGDAATCC	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11376_Sspa_Oph2007_COI	TSVA-031	1	BE11376_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	GAAGGCCTTCCGATGATC	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11376_Upin_Bot2011_COI	TSVA-031	1	BE11376_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	CTTTAATTACAGCGTTTTTATTGTTGT	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11376_Vgib_Wil2025_COI	TSVA-031	1	BE11376_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	CCCCTCTTTACAAGTTCTTTAGGGT	146.83336
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11377_Asen_Wil2025_COI	TSVA-032	1	BE11377_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	GTTGTTGTTACAACACATGCTCTTTTG	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11377_Cjap_Sim2018_COI	TSVA-032	1	BE11377_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11377_Esin_And2018_Cyb	TSVA-032	1	BE11377_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-032	Cytb	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	ACCCCTCCTCATATCCAACCA	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11377_Mgig_Bot2012_COI	TSVA-032	1	BE11377_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	TCTTATTCGTTGGAGACTTTATAACCCT	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11377_Mjap_Gib2022_COI	TSVA-032	1	BE11377_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	AATTTAATTGTGACTAGGCATGGTCTG	146.83341
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11378_Cmae_Bot2011_COI	TSVA-033	1	BE11378_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	ATGAACAGTCTATCCTCCTTTAG	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11378_Hsan_Wil2021_COI	TSVA-033	1	BE11378_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	CCTGGGCCGGTATAGTAGGT	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11378_Mros_Gib2022_COI	TSVA-033	1	BE11378_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11378_Msal_Bot2012_COI	TSVA-033	1	BE11378_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	GYTAGTTCCRATGATGTTAGCTG	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11378_Mstr_Wil2021_COI	TSVA-033	1	BE11378_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	GTAGTGGTAACTACTCATGCTTTA	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11378_Pvir_Dia2013_COI	TSVA-033	1	BE11378_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	CTTAGTGGCATTAATTCGDAATCC	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11378_Rhar_Sim2018_COI	TSVA-033	1	BE11378_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	CTGCTGCTATTGCTCATG	146.83416
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11380_Asen_Wil2025_COI	TSVA-035	1	BE11380_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	GTTGTTGTTACAACACATGCTCTTTTG	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11380_Cjap_Sim2018_COI	TSVA-035	1	BE11380_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11380_Esin_And2018_Cyb	TSVA-035	1	BE11380_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-035	Cytb	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	ACCCCTCCTCATATCCAACCA	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11380_Mgig_Bot2012_COI	TSVA-035	1	BE11380_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	TCTTATTCGTTGGAGACTTTATAACCCT	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11380_Mjap_Gib2022_COI	TSVA-035	1	BE11380_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	AATTTAATTGTGACTAGGCATGGTCTG	146.83379
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11385_Cmae_Bot2011_COI	GLTA-001	1	BE11385_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	ATGAACAGTCTATCCTCCTTTAG	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11385_Hsan_Wil2021_COI	GLTA-001	1	BE11385_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	CCTGGGCCGGTATAGTAGGT	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11385_Mros_Gib2022_COI	GLTA-001	1	BE11385_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11385_Msal_Bot2012_COI	GLTA-001	1	BE11385_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	GYTAGTTCCRATGATGTTAGCTG	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11385_Mstr_Wil2021_COI	GLTA-001	1	BE11385_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	GTAGTGGTAACTACTCATGCTTTA	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11385_Pvir_Dia2013_COI	GLTA-001	1	BE11385_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	CTTAGTGGCATTAATTCGDAATCC	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11385_Rhar_Sim2018_COI	GLTA-001	1	BE11385_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	CTGCTGCTATTGCTCATG	151.32361
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11387_Aamu_Bax2006_COI	GLTA-003	1	BE11387_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	GCACAACCGGGATCTTTACTTC	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11387_Pcan_Bot2011_IGS	GLTA-003	1	BE11387_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-003	IGS	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	CGTAATCCTCAGTACTGGCTA	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11387_Pper_Dia2013_COI	GLTA-003	1	BE11387_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	CTTAGTGGCATTAATTCGDAATCC	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11387_Sspa_Oph2007_COI	GLTA-003	1	BE11387_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	GAAGGCCTTCCGATGATC	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11387_Upin_Bot2011_COI	GLTA-003	1	BE11387_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	CTTTAATTACAGCGTTTTTATTGTTGT	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11387_Vgib_Wil2025_COI	GLTA-003	1	BE11387_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	CCCCTCTTTACAAGTTCTTTAGGGT	151.32533
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11388_Asen_Wil2025_COI	GLTA-004	1	BE11388_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	GTTGTTGTTACAACACATGCTCTTTTG	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11388_Cjap_Sim2018_COI	GLTA-004	1	BE11388_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11388_Esin_And2018_Cyb	GLTA-004	1	BE11388_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-004	Cytb	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	ACCCCTCCTCATATCCAACCA	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11388_Mgig_Bot2012_COI	GLTA-004	1	BE11388_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	TCTTATTCGTTGGAGACTTTATAACCCT	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11388_Mjap_Gib2022_COI	GLTA-004	1	BE11388_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	AATTTAATTGTGACTAGGCATGGTCTG	151.32448
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11389_Cmae_Bot2011_COI	GLTA-005	1	BE11389_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	ATGAACAGTCTATCCTCCTTTAG	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11389_Hsan_Wil2021_COI	GLTA-005	1	BE11389_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	CCTGGGCCGGTATAGTAGGT	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11389_Mros_Gib2022_COI	GLTA-005	1	BE11389_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11389_Msal_Bot2012_COI	GLTA-005	1	BE11389_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	GYTAGTTCCRATGATGTTAGCTG	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11389_Mstr_Wil2021_COI	GLTA-005	1	BE11389_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	GTAGTGGTAACTACTCATGCTTTA	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11389_Pvir_Dia2013_COI	GLTA-005	1	BE11389_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	CTTAGTGGCATTAATTCGDAATCC	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11389_Rhar_Sim2018_COI	GLTA-005	1	BE11389_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	CTGCTGCTATTGCTCATG	151.32614
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11390_Aamu_Bax2006_COI	GLTA-006	1	BE11390_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	GCACAACCGGGATCTTTACTTC	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11390_Pcan_Bot2011_IGS	GLTA-006	1	BE11390_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-006	IGS	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	CGTAATCCTCAGTACTGGCTA	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11390_Pper_Dia2013_COI	GLTA-006	1	BE11390_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	CTTAGTGGCATTAATTCGDAATCC	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11390_Sspa_Oph2007_COI	GLTA-006	1	BE11390_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	GAAGGCCTTCCGATGATC	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11390_Upin_Bot2011_COI	GLTA-006	1	BE11390_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	CTTTAATTACAGCGTTTTTATTGTTGT	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11390_Vgib_Wil2025_COI	GLTA-006	1	BE11390_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	CCCCTCTTTACAAGTTCTTTAGGGT	151.32283
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11391_Asen_Wil2025_COI	GLTA-007	1	BE11391_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	GTTGTTGTTACAACACATGCTCTTTTG	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11391_Cjap_Sim2018_COI	GLTA-007	1	BE11391_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11391_Esin_And2018_Cyb	GLTA-007	1	BE11391_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-007	Cytb	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	ACCCCTCCTCATATCCAACCA	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11391_Mgig_Bot2012_COI	GLTA-007	1	BE11391_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	TCTTATTCGTTGGAGACTTTATAACCCT	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11391_Mjap_Gib2022_COI	GLTA-007	1	BE11391_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	AATTTAATTGTGACTAGGCATGGTCTG	151.32495
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11392_Cmae_Bot2011_COI	GLTA-008	1	BE11392_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	ATGAACAGTCTATCCTCCTTTAG	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11392_Hsan_Wil2021_COI	GLTA-008	1	BE11392_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	CCTGGGCCGGTATAGTAGGT	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11392_Mros_Gib2022_COI	GLTA-008	1	BE11392_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11392_Msal_Bot2012_COI	GLTA-008	1	BE11392_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	GYTAGTTCCRATGATGTTAGCTG	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11392_Mstr_Wil2021_COI	GLTA-008	1	BE11392_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	GTAGTGGTAACTACTCATGCTTTA	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11392_Pvir_Dia2013_COI	GLTA-008	1	BE11392_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	CTTAGTGGCATTAATTCGDAATCC	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11392_Rhar_Sim2018_COI	GLTA-008	1	BE11392_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	CTGCTGCTATTGCTCATG	151.31684
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11394_Aamu_Bax2006_COI	GLTA-010	1	BE11394_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	GCACAACCGGGATCTTTACTTC	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11394_Pcan_Bot2011_IGS	GLTA-010	1	BE11394_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-010	IGS	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	CGTAATCCTCAGTACTGGCTA	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11394_Pper_Dia2013_COI	GLTA-010	1	BE11394_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	CTTAGTGGCATTAATTCGDAATCC	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11394_Sspa_Oph2007_COI	GLTA-010	1	BE11394_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	GAAGGCCTTCCGATGATC	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11394_Upin_Bot2011_COI	GLTA-010	1	BE11394_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	CTTTAATTACAGCGTTTTTATTGTTGT	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11394_Vgib_Wil2025_COI	GLTA-010	1	BE11394_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	CCCCTCTTTACAAGTTCTTTAGGGT	151.32062
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11395_Asen_Wil2025_COI	GLTA-011	1	BE11395_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	GTTGTTGTTACAACACATGCTCTTTTG	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11395_Cjap_Sim2018_COI	GLTA-011	1	BE11395_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11395_Esin_And2018_Cyb	GLTA-011	1	BE11395_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-011	Cytb	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	ACCCCTCCTCATATCCAACCA	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11395_Mgig_Bot2012_COI	GLTA-011	1	BE11395_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	TCTTATTCGTTGGAGACTTTATAACCCT	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11395_Mjap_Gib2022_COI	GLTA-011	1	BE11395_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	AATTTAATTGTGACTAGGCATGGTCTG	151.32214
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11396_Cmae_Bot2011_COI	GLTA-012	1	BE11396_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	ATGAACAGTCTATCCTCCTTTAG	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11396_Hsan_Wil2021_COI	GLTA-012	1	BE11396_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	CCTGGGCCGGTATAGTAGGT	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11396_Mros_Gib2022_COI	GLTA-012	1	BE11396_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11396_Msal_Bot2012_COI	GLTA-012	1	BE11396_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	GYTAGTTCCRATGATGTTAGCTG	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11396_Mstr_Wil2021_COI	GLTA-012	1	BE11396_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	GTAGTGGTAACTACTCATGCTTTA	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11396_Pvir_Dia2013_COI	GLTA-012	1	BE11396_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	CTTAGTGGCATTAATTCGDAATCC	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11396_Rhar_Sim2018_COI	GLTA-012	1	BE11396_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	CTGCTGCTATTGCTCATG	151.32342
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11398_Aamu_Bax2006_COI	GLTA-014	1	BE11398_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	GCACAACCGGGATCTTTACTTC	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11398_Pcan_Bot2011_IGS	GLTA-014	1	BE11398_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-014	IGS	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	CGTAATCCTCAGTACTGGCTA	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11398_Pper_Dia2013_COI	GLTA-014	1	BE11398_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	CTTAGTGGCATTAATTCGDAATCC	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11398_Sspa_Oph2007_COI	GLTA-014	1	BE11398_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	GAAGGCCTTCCGATGATC	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11398_Upin_Bot2011_COI	GLTA-014	1	BE11398_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	CTTTAATTACAGCGTTTTTATTGTTGT	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11398_Vgib_Wil2025_COI	GLTA-014	1	BE11398_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	CCCCTCTTTACAAGTTCTTTAGGGT	151.31563
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11399_Asen_Wil2025_COI	GLTA-015	1	BE11399_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	GTTGTTGTTACAACACATGCTCTTTTG	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11399_Cjap_Sim2018_COI	GLTA-015	1	BE11399_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11399_Esin_And2018_Cyb	GLTA-015	1	BE11399_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-015	Cytb	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	ACCCCTCCTCATATCCAACCA	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11399_Mgig_Bot2012_COI	GLTA-015	1	BE11399_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	TCTTATTCGTTGGAGACTTTATAACCCT	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11399_Mjap_Gib2022_COI	GLTA-015	1	BE11399_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	AATTTAATTGTGACTAGGCATGGTCTG	151.31768
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11400_Cmae_Bot2011_COI	GLTA-016	1	BE11400_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	ATGAACAGTCTATCCTCCTTTAG	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11400_Hsan_Wil2021_COI	GLTA-016	1	BE11400_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	CCTGGGCCGGTATAGTAGGT	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11400_Mros_Gib2022_COI	GLTA-016	1	BE11400_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11400_Msal_Bot2012_COI	GLTA-016	1	BE11400_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	GYTAGTTCCRATGATGTTAGCTG	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11400_Mstr_Wil2021_COI	GLTA-016	1	BE11400_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	GTAGTGGTAACTACTCATGCTTTA	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11400_Pvir_Dia2013_COI	GLTA-016	1	BE11400_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	CTTAGTGGCATTAATTCGDAATCC	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11400_Rhar_Sim2018_COI	GLTA-016	1	BE11400_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	CTGCTGCTATTGCTCATG	151.31914
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11402_Aamu_Bax2006_COI	GLTA-018	1	BE11402_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	GCACAACCGGGATCTTTACTTC	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11402_Pcan_Bot2011_IGS	GLTA-018	1	BE11402_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-018	IGS	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	CGTAATCCTCAGTACTGGCTA	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11402_Pper_Dia2013_COI	GLTA-018	1	BE11402_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	CTTAGTGGCATTAATTCGDAATCC	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11402_Sspa_Oph2007_COI	GLTA-018	1	BE11402_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	GAAGGCCTTCCGATGATC	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11402_Upin_Bot2011_COI	GLTA-018	1	BE11402_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	CTTTAATTACAGCGTTTTTATTGTTGT	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11402_Vgib_Wil2025_COI	GLTA-018	1	BE11402_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	CCCCTCTTTACAAGTTCTTTAGGGT	151.32283
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11403_Asen_Wil2025_COI	GLTA-019	1	BE11403_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	GTTGTTGTTACAACACATGCTCTTTTG	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11403_Cjap_Sim2018_COI	GLTA-019	1	BE11403_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11403_Esin_And2018_Cyb	GLTA-019	1	BE11403_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-019	Cytb	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	ACCCCTCCTCATATCCAACCA	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11403_Mgig_Bot2012_COI	GLTA-019	1	BE11403_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	TCTTATTCGTTGGAGACTTTATAACCCT	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11403_Mjap_Gib2022_COI	GLTA-019	1	BE11403_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	AATTTAATTGTGACTAGGCATGGTCTG	151.30614
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11404_Cmae_Bot2011_COI	GLTA-020	1	BE11404_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	ATGAACAGTCTATCCTCCTTTAG	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11404_Hsan_Wil2021_COI	GLTA-020	1	BE11404_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	CCTGGGCCGGTATAGTAGGT	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11404_Mros_Gib2022_COI	GLTA-020	1	BE11404_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11404_Msal_Bot2012_COI	GLTA-020	1	BE11404_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	GYTAGTTCCRATGATGTTAGCTG	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11404_Mstr_Wil2021_COI	GLTA-020	1	BE11404_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	GTAGTGGTAACTACTCATGCTTTA	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11404_Pvir_Dia2013_COI	GLTA-020	1	BE11404_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	CTTAGTGGCATTAATTCGDAATCC	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11404_Rhar_Sim2018_COI	GLTA-020	1	BE11404_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	CTGCTGCTATTGCTCATG	151.308
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11406_Aamu_Bax2006_COI	GLTA-022	1	BE11406_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	GCACAACCGGGATCTTTACTTC	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11406_Pcan_Bot2011_IGS	GLTA-022	1	BE11406_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-022	IGS	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	CGTAATCCTCAGTACTGGCTA	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11406_Pper_Dia2013_COI	GLTA-022	1	BE11406_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	CTTAGTGGCATTAATTCGDAATCC	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11406_Sspa_Oph2007_COI	GLTA-022	1	BE11406_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	GAAGGCCTTCCGATGATC	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11406_Upin_Bot2011_COI	GLTA-022	1	BE11406_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	CTTTAATTACAGCGTTTTTATTGTTGT	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11406_Vgib_Wil2025_COI	GLTA-022	1	BE11406_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	CCCCTCTTTACAAGTTCTTTAGGGT	151.31087
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11407_Asen_Wil2025_COI	GLTA-023	1	BE11407_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	GTTGTTGTTACAACACATGCTCTTTTG	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11407_Cjap_Sim2018_COI	GLTA-023	1	BE11407_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11407_Esin_And2018_Cyb	GLTA-023	1	BE11407_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-023	Cytb	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	ACCCCTCCTCATATCCAACCA	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11407_Mgig_Bot2012_COI	GLTA-023	1	BE11407_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	TCTTATTCGTTGGAGACTTTATAACCCT	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11407_Mjap_Gib2022_COI	GLTA-023	1	BE11407_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	AATTTAATTGTGACTAGGCATGGTCTG	151.31293
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11408_Cmae_Bot2011_COI	GLTA-024	1	BE11408_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	ATGAACAGTCTATCCTCCTTTAG	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11408_Hsan_Wil2021_COI	GLTA-024	1	BE11408_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	CCTGGGCCGGTATAGTAGGT	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11408_Mros_Gib2022_COI	GLTA-024	1	BE11408_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11408_Msal_Bot2012_COI	GLTA-024	1	BE11408_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	GYTAGTTCCRATGATGTTAGCTG	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11408_Mstr_Wil2021_COI	GLTA-024	1	BE11408_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	GTAGTGGTAACTACTCATGCTTTA	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11408_Pvir_Dia2013_COI	GLTA-024	1	BE11408_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	CTTAGTGGCATTAATTCGDAATCC	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11408_Rhar_Sim2018_COI	GLTA-024	1	BE11408_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	CTGCTGCTATTGCTCATG	151.31454
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11410_Asen_Wil2025_COI	GLTA-026	1	BE11410_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	GTTGTTGTTACAACACATGCTCTTTTG	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11410_Cjap_Sim2018_COI	GLTA-026	1	BE11410_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11410_Esin_And2018_Cyb	GLTA-026	1	BE11410_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-026	Cytb	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	ACCCCTCCTCATATCCAACCA	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11410_Mgig_Bot2012_COI	GLTA-026	1	BE11410_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	TCTTATTCGTTGGAGACTTTATAACCCT	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11410_Mjap_Gib2022_COI	GLTA-026	1	BE11410_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	AATTTAATTGTGACTAGGCATGGTCTG	151.31826
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11411_Cmae_Bot2011_COI	GLTA-027	1	BE11411_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	ATGAACAGTCTATCCTCCTTTAG	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11411_Hsan_Wil2021_COI	GLTA-027	1	BE11411_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	CCTGGGCCGGTATAGTAGGT	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11411_Mros_Gib2022_COI	GLTA-027	1	BE11411_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11411_Msal_Bot2012_COI	GLTA-027	1	BE11411_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	GYTAGTTCCRATGATGTTAGCTG	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11411_Mstr_Wil2021_COI	GLTA-027	1	BE11411_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	GTAGTGGTAACTACTCATGCTTTA	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11411_Pvir_Dia2013_COI	GLTA-027	1	BE11411_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	CTTAGTGGCATTAATTCGDAATCC	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11411_Rhar_Sim2018_COI	GLTA-027	1	BE11411_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	CTGCTGCTATTGCTCATG	151.31994
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11413_Aamu_Bax2006_COI	GLTA-029	1	BE11413_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	GCACAACCGGGATCTTTACTTC	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11413_Pcan_Bot2011_IGS	GLTA-029	1	BE11413_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-029	IGS	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	CGTAATCCTCAGTACTGGCTA	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11413_Pper_Dia2013_COI	GLTA-029	1	BE11413_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	CTTAGTGGCATTAATTCGDAATCC	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11413_Sspa_Oph2007_COI	GLTA-029	1	BE11413_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	GAAGGCCTTCCGATGATC	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11413_Upin_Bot2011_COI	GLTA-029	1	BE11413_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	CTTTAATTACAGCGTTTTTATTGTTGT	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11413_Vgib_Wil2025_COI	GLTA-029	1	BE11413_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	CCCCTCTTTACAAGTTCTTTAGGGT	151.3087
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11414_Asen_Wil2025_COI	GLTA-030	1	BE11414_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	GTTGTTGTTACAACACATGCTCTTTTG	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11414_Cjap_Sim2018_COI	GLTA-030	1	BE11414_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11414_Esin_And2018_Cyb	GLTA-030	1	BE11414_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-030	Cytb	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	ACCCCTCCTCATATCCAACCA	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11414_Mgig_Bot2012_COI	GLTA-030	1	BE11414_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	TCTTATTCGTTGGAGACTTTATAACCCT	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11414_Mjap_Gib2022_COI	GLTA-030	1	BE11414_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	AATTTAATTGTGACTAGGCATGGTCTG	151.31051
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11415_Cmae_Bot2011_COI	GLTA-031	1	BE11415_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	ATGAACAGTCTATCCTCCTTTAG	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11415_Mros_Gib2022_COI	GLTA-031	1	BE11415_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11415_Msal_Bot2012_COI	GLTA-031	1	BE11415_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	GYTAGTTCCRATGATGTTAGCTG	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11415_Mstr_Wil2021_COI	GLTA-031	1	BE11415_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	GTAGTGGTAACTACTCATGCTTTA	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11415_Pvir_Dia2013_COI	GLTA-031	1	BE11415_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	CTTAGTGGCATTAATTCGDAATCC	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11415_Rhar_Sim2018_COI	GLTA-031	1	BE11415_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	CTGCTGCTATTGCTCATG	151.3121
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11417_Aamu_Bax2006_COI	GLTA-033	1	BE11417_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	GCACAACCGGGATCTTTACTTC	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11417_Pcan_Bot2011_IGS	GLTA-033	1	BE11417_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-033	IGS	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	CGTAATCCTCAGTACTGGCTA	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11417_Pper_Dia2013_COI	GLTA-033	1	BE11417_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	CTTAGTGGCATTAATTCGDAATCC	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11417_Sspa_Oph2007_COI	GLTA-033	1	BE11417_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	GAAGGCCTTCCGATGATC	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11417_Upin_Bot2011_COI	GLTA-033	1	BE11417_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	CTTTAATTACAGCGTTTTTATTGTTGT	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11417_Vgib_Wil2025_COI	GLTA-033	1	BE11417_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	CCCCTCTTTACAAGTTCTTTAGGGT	151.3157
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11418_Asen_Wil2025_COI	GLTA-034	1	BE11418_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	GTTGTTGTTACAACACATGCTCTTTTG	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11418_Cjap_Sim2018_COI	GLTA-034	1	BE11418_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11418_Esin_And2018_Cyb	GLTA-034	1	BE11418_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-034	Cytb	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	ACCCCTCCTCATATCCAACCA	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11418_Mgig_Bot2012_COI	GLTA-034	1	BE11418_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	TCTTATTCGTTGGAGACTTTATAACCCT	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11418_Mjap_Gib2022_COI	GLTA-034	1	BE11418_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	AATTTAATTGTGACTAGGCATGGTCTG	151.31106
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11419_Cmae_Bot2011_COI	GLTA-035	1	BE11419_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	ATGAACAGTCTATCCTCCTTTAG	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11419_Hsan_Wil2021_COI	GLTA-035	1	BE11419_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	CCTGGGCCGGTATAGTAGGT	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11419_Mros_Gib2022_COI	GLTA-035	1	BE11419_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11419_Msal_Bot2012_COI	GLTA-035	1	BE11419_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	GYTAGTTCCRATGATGTTAGCTG	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11419_Mstr_Wil2021_COI	GLTA-035	1	BE11419_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	GTAGTGGTAACTACTCATGCTTTA	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11419_Pvir_Dia2013_COI	GLTA-035	1	BE11419_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	CTTAGTGGCATTAATTCGDAATCC	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11419_Rhar_Sim2018_COI	GLTA-035	1	BE11419_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	CTGCTGCTATTGCTCATG	151.31275
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11424_Aamu_Bax2006_COI	GLTA-036	1	BE11424_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	GCACAACCGGGATCTTTACTTC	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11424_Pcan_Bot2011_IGS	GLTA-036	1	BE11424_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-036	IGS	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	CGTAATCCTCAGTACTGGCTA	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11424_Pper_Dia2013_COI	GLTA-036	1	BE11424_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	CTTAGTGGCATTAATTCGDAATCC	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11424_Sspa_Oph2007_COI	GLTA-036	1	BE11424_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	GAAGGCCTTCCGATGATC	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11424_Upin_Bot2011_COI	GLTA-036	1	BE11424_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	CTTTAATTACAGCGTTTTTATTGTTGT	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11424_Vgib_Wil2025_COI	GLTA-036	1	BE11424_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	CCCCTCTTTACAAGTTCTTTAGGGT	151.27517
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11425_Asen_Wil2025_COI	GLTA-037	1	BE11425_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	GTTGTTGTTACAACACATGCTCTTTTG	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11425_Cjap_Sim2018_COI	GLTA-037	1	BE11425_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11425_Esin_And2018_Cyb	GLTA-037	1	BE11425_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-037	Cytb	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	ACCCCTCCTCATATCCAACCA	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11425_Mgig_Bot2012_COI	GLTA-037	1	BE11425_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	TCTTATTCGTTGGAGACTTTATAACCCT	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11425_Mjap_Gib2022_COI	GLTA-037	1	BE11425_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	AATTTAATTGTGACTAGGCATGGTCTG	151.27401
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11426_Cmae_Bot2011_COI	GLTA-038	1	BE11426_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	ATGAACAGTCTATCCTCCTTTAG	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11426_Hsan_Wil2021_COI	GLTA-038	1	BE11426_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	CCTGGGCCGGTATAGTAGGT	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11426_Mros_Gib2022_COI	GLTA-038	1	BE11426_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11426_Msal_Bot2012_COI	GLTA-038	1	BE11426_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	GYTAGTTCCRATGATGTTAGCTG	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11426_Mstr_Wil2021_COI	GLTA-038	1	BE11426_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	GTAGTGGTAACTACTCATGCTTTA	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11426_Pvir_Dia2013_COI	GLTA-038	1	BE11426_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	CTTAGTGGCATTAATTCGDAATCC	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11426_Rhar_Sim2018_COI	GLTA-038	1	BE11426_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	CTGCTGCTATTGCTCATG	151.27728
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11428_Aamu_Bax2006_COI	GLTA-040	1	BE11428_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	GCACAACCGGGATCTTTACTTC	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11428_Pcan_Bot2011_IGS	GLTA-040	1	BE11428_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-040	IGS	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	CGTAATCCTCAGTACTGGCTA	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11428_Pper_Dia2013_COI	GLTA-040	1	BE11428_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	CTTAGTGGCATTAATTCGDAATCC	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11428_Sspa_Oph2007_COI	GLTA-040	1	BE11428_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	GAAGGCCTTCCGATGATC	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11428_Upin_Bot2011_COI	GLTA-040	1	BE11428_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	CTTTAATTACAGCGTTTTTATTGTTGT	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11428_Vgib_Wil2025_COI	GLTA-040	1	BE11428_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	CCCCTCTTTACAAGTTCTTTAGGGT	151.2752
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11429_Asen_Wil2025_COI	GLTA-041	1	BE11429_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	GTTGTTGTTACAACACATGCTCTTTTG	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11429_Cjap_Sim2018_COI	GLTA-041	1	BE11429_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11429_Esin_And2018_Cyb	GLTA-041	1	BE11429_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-041	Cytb	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	ACCCCTCCTCATATCCAACCA	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11429_Mgig_Bot2012_COI	GLTA-041	1	BE11429_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	TCTTATTCGTTGGAGACTTTATAACCCT	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11429_Mjap_Gib2022_COI	GLTA-041	1	BE11429_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	AATTTAATTGTGACTAGGCATGGTCTG	151.27704
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11431_Aamu_Bax2006_COI	GLTA-043	1	BE11431_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	GCACAACCGGGATCTTTACTTC	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11431_Pcan_Bot2011_IGS	GLTA-043	1	BE11431_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-043	IGS	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	CGTAATCCTCAGTACTGGCTA	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11431_Pper_Dia2013_COI	GLTA-043	1	BE11431_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	CTTAGTGGCATTAATTCGDAATCC	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11431_Sspa_Oph2007_COI	GLTA-043	1	BE11431_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	GAAGGCCTTCCGATGATC	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11431_Upin_Bot2011_COI	GLTA-043	1	BE11431_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	CTTTAATTACAGCGTTTTTATTGTTGT	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11431_Vgib_Wil2025_COI	GLTA-043	1	BE11431_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	CCCCTCTTTACAAGTTCTTTAGGGT	151.26895
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11432_Asen_Wil2025_COI	GLTA-044	1	BE11432_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	GTTGTTGTTACAACACATGCTCTTTTG	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11432_Cjap_Sim2018_COI	GLTA-044	1	BE11432_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11432_Esin_And2018_Cyb	GLTA-044	1	BE11432_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-044	Cytb	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	ACCCCTCCTCATATCCAACCA	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11432_Mgig_Bot2012_COI	GLTA-044	1	BE11432_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	TCTTATTCGTTGGAGACTTTATAACCCT	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11432_Mjap_Gib2022_COI	GLTA-044	1	BE11432_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	AATTTAATTGTGACTAGGCATGGTCTG	151.27076
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11433_Cmae_Bot2011_COI	GLTA-045	1	BE11433_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	ATGAACAGTCTATCCTCCTTTAG	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11433_Hsan_Wil2021_COI	GLTA-045	1	BE11433_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	CCTGGGCCGGTATAGTAGGT	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11433_Mros_Gib2022_COI	GLTA-045	1	BE11433_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11433_Msal_Bot2012_COI	GLTA-045	1	BE11433_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	GYTAGTTCCRATGATGTTAGCTG	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11433_Mstr_Wil2021_COI	GLTA-045	1	BE11433_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	GTAGTGGTAACTACTCATGCTTTA	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11433_Pvir_Dia2013_COI	GLTA-045	1	BE11433_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	CTTAGTGGCATTAATTCGDAATCC	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11433_Rhar_Sim2018_COI	GLTA-045	1	BE11433_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	CTGCTGCTATTGCTCATG	151.27266
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11435_Aamu_Bax2006_COI	GLTA-047	1	BE11435_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	GCACAACCGGGATCTTTACTTC	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11435_Pcan_Bot2011_IGS	GLTA-047	1	BE11435_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-047	IGS	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	CGTAATCCTCAGTACTGGCTA	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11435_Pper_Dia2013_COI	GLTA-047	1	BE11435_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	CTTAGTGGCATTAATTCGDAATCC	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11435_Sspa_Oph2007_COI	GLTA-047	1	BE11435_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	GAAGGCCTTCCGATGATC	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11435_Upin_Bot2011_COI	GLTA-047	1	BE11435_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	CTTTAATTACAGCGTTTTTATTGTTGT	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11435_Vgib_Wil2025_COI	GLTA-047	1	BE11435_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	CCCCTCTTTACAAGTTCTTTAGGGT	151.27718
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11436_Asen_Wil2025_COI	GLTA-048	1	BE11436_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	GTTGTTGTTACAACACATGCTCTTTTG	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11436_Cjap_Sim2018_COI	GLTA-048	1	BE11436_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11436_Esin_And2018_Cyb	GLTA-048	1	BE11436_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-048	Cytb	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	ACCCCTCCTCATATCCAACCA	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11436_Mgig_Bot2012_COI	GLTA-048	1	BE11436_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	TCTTATTCGTTGGAGACTTTATAACCCT	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11436_Mjap_Gib2022_COI	GLTA-048	1	BE11436_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	AATTTAATTGTGACTAGGCATGGTCTG	151.26603
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11437_Cmae_Bot2011_COI	GLTA-049	1	BE11437_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	ATGAACAGTCTATCCTCCTTTAG	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11437_Hsan_Wil2021_COI	GLTA-049	1	BE11437_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	CCTGGGCCGGTATAGTAGGT	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11437_Mros_Gib2022_COI	GLTA-049	1	BE11437_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11437_Msal_Bot2012_COI	GLTA-049	1	BE11437_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	GYTAGTTCCRATGATGTTAGCTG	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11437_Mstr_Wil2021_COI	GLTA-049	1	BE11437_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	GTAGTGGTAACTACTCATGCTTTA	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11437_Pvir_Dia2013_COI	GLTA-049	1	BE11437_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	CTTAGTGGCATTAATTCGDAATCC	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11437_Rhar_Sim2018_COI	GLTA-049	1	BE11437_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	CTGCTGCTATTGCTCATG	151.26763
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11439_Aamu_Bax2006_COI	GLTA-051	1	BE11439_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	GCACAACCGGGATCTTTACTTC	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11439_Pcan_Bot2011_IGS	GLTA-051	1	BE11439_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-051	IGS	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	CGTAATCCTCAGTACTGGCTA	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11439_Pper_Dia2013_COI	GLTA-051	1	BE11439_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	CTTAGTGGCATTAATTCGDAATCC	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11439_Sspa_Oph2007_COI	GLTA-051	1	BE11439_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	GAAGGCCTTCCGATGATC	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11439_Upin_Bot2011_COI	GLTA-051	1	BE11439_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	CTTTAATTACAGCGTTTTTATTGTTGT	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11439_Vgib_Wil2025_COI	GLTA-051	1	BE11439_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	CCCCTCTTTACAAGTTCTTTAGGGT	151.27186
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11440_Cmae_Bot2011_COI	GLTA-052	1	BE11440_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	ATGAACAGTCTATCCTCCTTTAG	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11440_Hsan_Wil2021_COI	GLTA-052	1	BE11440_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	CCTGGGCCGGTATAGTAGGT	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11440_Mros_Gib2022_COI	GLTA-052	1	BE11440_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11440_Msal_Bot2012_COI	GLTA-052	1	BE11440_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	GYTAGTTCCRATGATGTTAGCTG	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11440_Mstr_Wil2021_COI	GLTA-052	1	BE11440_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	GTAGTGGTAACTACTCATGCTTTA	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11440_Pvir_Dia2013_COI	GLTA-052	1	BE11440_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	CTTAGTGGCATTAATTCGDAATCC	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11440_Rhar_Sim2018_COI	GLTA-052	1	BE11440_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	CTGCTGCTATTGCTCATG	151.27336
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11442_Aamu_Bax2006_COI	GLTA-054	1	BE11442_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	GCACAACCGGGATCTTTACTTC	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11442_Pcan_Bot2011_IGS	GLTA-054	1	BE11442_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-054	IGS	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	CGTAATCCTCAGTACTGGCTA	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11442_Pper_Dia2013_COI	GLTA-054	1	BE11442_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	CTTAGTGGCATTAATTCGDAATCC	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11442_Sspa_Oph2007_COI	GLTA-054	1	BE11442_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	GAAGGCCTTCCGATGATC	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11442_Upin_Bot2011_COI	GLTA-054	1	BE11442_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	CTTTAATTACAGCGTTTTTATTGTTGT	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11442_Vgib_Wil2025_COI	GLTA-054	1	BE11442_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	CCCCTCTTTACAAGTTCTTTAGGGT	151.26466
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11443_Asen_Wil2025_COI	GLTA-055	1	BE11443_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	GTTGTTGTTACAACACATGCTCTTTTG	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11443_Cjap_Sim2018_COI	GLTA-055	1	BE11443_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11443_Esin_And2018_Cyb	GLTA-055	1	BE11443_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-055	Cytb	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	ACCCCTCCTCATATCCAACCA	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11443_Mgig_Bot2012_COI	GLTA-055	1	BE11443_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	TCTTATTCGTTGGAGACTTTATAACCCT	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11443_Mjap_Gib2022_COI	GLTA-055	1	BE11443_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	AATTTAATTGTGACTAGGCATGGTCTG	151.26634
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11444_Cmae_Bot2011_COI	GLTA-056	1	BE11444_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	ATGAACAGTCTATCCTCCTTTAG	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11444_Hsan_Wil2021_COI	GLTA-056	1	BE11444_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	CCTGGGCCGGTATAGTAGGT	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11444_Mros_Gib2022_COI	GLTA-056	1	BE11444_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11444_Msal_Bot2012_COI	GLTA-056	1	BE11444_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	GYTAGTTCCRATGATGTTAGCTG	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11444_Mstr_Wil2021_COI	GLTA-056	1	BE11444_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	GTAGTGGTAACTACTCATGCTTTA	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11444_Pvir_Dia2013_COI	GLTA-056	1	BE11444_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	CTTAGTGGCATTAATTCGDAATCC	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11444_Rhar_Sim2018_COI	GLTA-056	1	BE11444_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	CTGCTGCTATTGCTCATG	151.26801
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11446_Aamu_Bax2006_COI	GLTA-058	1	BE11446_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	GCACAACCGGGATCTTTACTTC	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11446_Pcan_Bot2011_IGS	GLTA-058	1	BE11446_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-058	IGS	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	CGTAATCCTCAGTACTGGCTA	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11446_Pper_Dia2013_COI	GLTA-058	1	BE11446_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	CTTAGTGGCATTAATTCGDAATCC	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11446_Sspa_Oph2007_COI	GLTA-058	1	BE11446_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	GAAGGCCTTCCGATGATC	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11446_Upin_Bot2011_COI	GLTA-058	1	BE11446_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	CTTTAATTACAGCGTTTTTATTGTTGT	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11446_Vgib_Wil2025_COI	GLTA-058	1	BE11446_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	CCCCTCTTTACAAGTTCTTTAGGGT	151.25936
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11447_Asen_Wil2025_COI	GLTA-059	1	BE11447_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	GTTGTTGTTACAACACATGCTCTTTTG	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11447_Cjap_Sim2018_COI	GLTA-059	1	BE11447_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11447_Esin_And2018_Cyb	GLTA-059	1	BE11447_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-059	Cytb	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	ACCCCTCCTCATATCCAACCA	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11447_Mgig_Bot2012_COI	GLTA-059	1	BE11447_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	TCTTATTCGTTGGAGACTTTATAACCCT	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11447_Mjap_Gib2022_COI	GLTA-059	1	BE11447_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	AATTTAATTGTGACTAGGCATGGTCTG	151.26129
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11448_Cmae_Bot2011_COI	GLTA-060	1	BE11448_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	ATGAACAGTCTATCCTCCTTTAG	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11448_Hsan_Wil2021_COI	GLTA-060	1	BE11448_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	CCTGGGCCGGTATAGTAGGT	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11448_Mros_Gib2022_COI	GLTA-060	1	BE11448_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11448_Msal_Bot2012_COI	GLTA-060	1	BE11448_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	GYTAGTTCCRATGATGTTAGCTG	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11448_Mstr_Wil2021_COI	GLTA-060	1	BE11448_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	GTAGTGGTAACTACTCATGCTTTA	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11448_Pvir_Dia2013_COI	GLTA-060	1	BE11448_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	CTTAGTGGCATTAATTCGDAATCC	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11448_Rhar_Sim2018_COI	GLTA-060	1	BE11448_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	CTGCTGCTATTGCTCATG	151.26294
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11450_Asen_Wil2025_COI	GLTA-062	1	BE11450_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	GTTGTTGTTACAACACATGCTCTTTTG	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11450_Cjap_Sim2018_COI	GLTA-062	1	BE11450_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11450_Esin_And2018_Cyb	GLTA-062	1	BE11450_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-062	Cytb	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	ACCCCTCCTCATATCCAACCA	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11450_Mgig_Bot2012_COI	GLTA-062	1	BE11450_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	TCTTATTCGTTGGAGACTTTATAACCCT	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11450_Mjap_Gib2022_COI	GLTA-062	1	BE11450_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	AATTTAATTGTGACTAGGCATGGTCTG	151.25286
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11451_Cmae_Bot2011_COI	GLTA-063	1	BE11451_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	ATGAACAGTCTATCCTCCTTTAG	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11451_Hsan_Wil2021_COI	GLTA-063	1	BE11451_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	CCTGGGCCGGTATAGTAGGT	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11451_Mros_Gib2022_COI	GLTA-063	1	BE11451_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11451_Msal_Bot2012_COI	GLTA-063	1	BE11451_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	GYTAGTTCCRATGATGTTAGCTG	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11451_Mstr_Wil2021_COI	GLTA-063	1	BE11451_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	GTAGTGGTAACTACTCATGCTTTA	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11451_Pvir_Dia2013_COI	GLTA-063	1	BE11451_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	CTTAGTGGCATTAATTCGDAATCC	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11451_Rhar_Sim2018_COI	GLTA-063	1	BE11451_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	CTGCTGCTATTGCTCATG	151.25459
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11453_Aamu_Bax2006_COI	GLTA-065	1	BE11453_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	GCACAACCGGGATCTTTACTTC	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11453_Pcan_Bot2011_IGS	GLTA-065	1	BE11453_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-065	IGS	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	CGTAATCCTCAGTACTGGCTA	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11453_Pper_Dia2013_COI	GLTA-065	1	BE11453_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	CTTAGTGGCATTAATTCGDAATCC	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11453_Sspa_Oph2007_COI	GLTA-065	1	BE11453_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	GAAGGCCTTCCGATGATC	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11453_Upin_Bot2011_COI	GLTA-065	1	BE11453_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	CTTTAATTACAGCGTTTTTATTGTTGT	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11453_Vgib_Wil2025_COI	GLTA-065	1	BE11453_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	CCCCTCTTTACAAGTTCTTTAGGGT	151.25786
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11454_Asen_Wil2025_COI	GLTA-066	1	BE11454_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	GTTGTTGTTACAACACATGCTCTTTTG	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11454_Cjap_Sim2018_COI	GLTA-066	1	BE11454_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11454_Esin_And2018_Cyb	GLTA-066	1	BE11454_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-066	Cytb	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	ACCCCTCCTCATATCCAACCA	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11454_Mgig_Bot2012_COI	GLTA-066	1	BE11454_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	TCTTATTCGTTGGAGACTTTATAACCCT	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11454_Mjap_Gib2022_COI	GLTA-066	1	BE11454_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	AATTTAATTGTGACTAGGCATGGTCTG	151.26022
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11455_Cmae_Bot2011_COI	GLTA-067	1	BE11455_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	ATGAACAGTCTATCCTCCTTTAG	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11455_Hsan_Wil2021_COI	GLTA-067	1	BE11455_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	CCTGGGCCGGTATAGTAGGT	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11455_Mros_Gib2022_COI	GLTA-067	1	BE11455_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11455_Msal_Bot2012_COI	GLTA-067	1	BE11455_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	GYTAGTTCCRATGATGTTAGCTG	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11455_Mstr_Wil2021_COI	GLTA-067	1	BE11455_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	GTAGTGGTAACTACTCATGCTTTA	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11455_Pvir_Dia2013_COI	GLTA-067	1	BE11455_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	CTTAGTGGCATTAATTCGDAATCC	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11455_Rhar_Sim2018_COI	GLTA-067	1	BE11455_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	CTGCTGCTATTGCTCATG	151.26156
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11457_Aamu_Bax2006_COI	GLTA-069	1	BE11457_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	GCACAACCGGGATCTTTACTTC	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11457_Pcan_Bot2011_IGS	GLTA-069	1	BE11457_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-069	IGS	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	CGTAATCCTCAGTACTGGCTA	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11457_Pper_Dia2013_COI	GLTA-069	1	BE11457_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	CTTAGTGGCATTAATTCGDAATCC	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11457_Sspa_Oph2007_COI	GLTA-069	1	BE11457_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	GAAGGCCTTCCGATGATC	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11457_Upin_Bot2011_COI	GLTA-069	1	BE11457_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	CTTTAATTACAGCGTTTTTATTGTTGT	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11457_Vgib_Wil2025_COI	GLTA-069	1	BE11457_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	CCCCTCTTTACAAGTTCTTTAGGGT	151.25516
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11458_Asen_Wil2025_COI	GLTA-070	1	BE11458_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	GTTGTTGTTACAACACATGCTCTTTTG	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11458_Cjap_Sim2018_COI	GLTA-070	1	BE11458_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11458_Esin_And2018_Cyb	GLTA-070	1	BE11458_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-070	Cytb	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	ACCCCTCCTCATATCCAACCA	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11458_Mgig_Bot2012_COI	GLTA-070	1	BE11458_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	TCTTATTCGTTGGAGACTTTATAACCCT	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11458_Mjap_Gib2022_COI	GLTA-070	1	BE11458_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	AATTTAATTGTGACTAGGCATGGTCTG	151.25701
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11464_Aamu_Bax2006_COI	GLTA-077	1	BE11464_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	GCACAACCGGGATCTTTACTTC	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11464_Pcan_Bot2011_IGS	GLTA-077	1	BE11464_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-077	IGS	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	CGTAATCCTCAGTACTGGCTA	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11464_Pper_Dia2013_COI	GLTA-077	1	BE11464_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	CTTAGTGGCATTAATTCGDAATCC	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11464_Sspa_Oph2007_COI	GLTA-077	1	BE11464_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	GAAGGCCTTCCGATGATC	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11464_Upin_Bot2011_COI	GLTA-077	1	BE11464_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	CTTTAATTACAGCGTTTTTATTGTTGT	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11464_Vgib_Wil2025_COI	GLTA-077	1	BE11464_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	CCCCTCTTTACAAGTTCTTTAGGGT	151.18717
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11465_Asen_Wil2025_COI	GLTA-078	1	BE11465_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	GTTGTTGTTACAACACATGCTCTTTTG	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11465_Cjap_Sim2018_COI	GLTA-078	1	BE11465_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11465_Esin_And2018_Cyb	GLTA-078	1	BE11465_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-078	Cytb	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	ACCCCTCCTCATATCCAACCA	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11465_Mgig_Bot2012_COI	GLTA-078	1	BE11465_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	TCTTATTCGTTGGAGACTTTATAACCCT	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11465_Mjap_Gib2022_COI	GLTA-078	1	BE11465_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	AATTTAATTGTGACTAGGCATGGTCTG	151.18864
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11466_Cmae_Bot2011_COI	GLTA-079	1	BE11466_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	ATGAACAGTCTATCCTCCTTTAG	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11466_Hsan_Wil2021_COI	GLTA-079	1	BE11466_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	CCTGGGCCGGTATAGTAGGT	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11466_Mros_Gib2022_COI	GLTA-079	1	BE11466_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11466_Msal_Bot2012_COI	GLTA-079	1	BE11466_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	GYTAGTTCCRATGATGTTAGCTG	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11466_Mstr_Wil2021_COI	GLTA-079	1	BE11466_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	GTAGTGGTAACTACTCATGCTTTA	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11466_Pvir_Dia2013_COI	GLTA-079	1	BE11466_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	CTTAGTGGCATTAATTCGDAATCC	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11466_Rhar_Sim2018_COI	GLTA-079	1	BE11466_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	CTGCTGCTATTGCTCATG	151.18113
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11468_Aamu_Bax2006_COI	GLTA-081	1	BE11468_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	GCACAACCGGGATCTTTACTTC	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11468_Pcan_Bot2011_IGS	GLTA-081	1	BE11468_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-081	IGS	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	CGTAATCCTCAGTACTGGCTA	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11468_Pper_Dia2013_COI	GLTA-081	1	BE11468_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	CTTAGTGGCATTAATTCGDAATCC	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11468_Sspa_Oph2007_COI	GLTA-081	1	BE11468_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	GAAGGCCTTCCGATGATC	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11468_Upin_Bot2011_COI	GLTA-081	1	BE11468_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	CTTTAATTACAGCGTTTTTATTGTTGT	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11468_Vgib_Wil2025_COI	GLTA-081	1	BE11468_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	CCCCTCTTTACAAGTTCTTTAGGGT	151.18752
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11469_Asen_Wil2025_COI	GLTA-082	1	BE11469_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	GTTGTTGTTACAACACATGCTCTTTTG	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11469_Cjap_Sim2018_COI	GLTA-082	1	BE11469_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11469_Esin_And2018_Cyb	GLTA-082	1	BE11469_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-082	Cytb	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	ACCCCTCCTCATATCCAACCA	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11469_Mgig_Bot2012_COI	GLTA-082	1	BE11469_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	TCTTATTCGTTGGAGACTTTATAACCCT	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11469_Mjap_Gib2022_COI	GLTA-082	1	BE11469_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	AATTTAATTGTGACTAGGCATGGTCTG	151.18347
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11471_Aamu_Bax2006_COI	GLTA-084	1	BE11471_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	GCACAACCGGGATCTTTACTTC	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11471_Pcan_Bot2011_IGS	GLTA-084	1	BE11471_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-084	IGS	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	CGTAATCCTCAGTACTGGCTA	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11471_Pper_Dia2013_COI	GLTA-084	1	BE11471_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	CTTAGTGGCATTAATTCGDAATCC	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11471_Sspa_Oph2007_COI	GLTA-084	1	BE11471_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	GAAGGCCTTCCGATGATC	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11471_Upin_Bot2011_COI	GLTA-084	1	BE11471_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	CTTTAATTACAGCGTTTTTATTGTTGT	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11471_Vgib_Wil2025_COI	GLTA-084	1	BE11471_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	CCCCTCTTTACAAGTTCTTTAGGGT	151.18654
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11472_Asen_Wil2025_COI	GLTA-085	1	BE11472_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	GTTGTTGTTACAACACATGCTCTTTTG	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11472_Cjap_Sim2018_COI	GLTA-085	1	BE11472_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11472_Esin_And2018_Cyb	GLTA-085	1	BE11472_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-085	Cytb	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	ACCCCTCCTCATATCCAACCA	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11472_Mgig_Bot2012_COI	GLTA-085	1	BE11472_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	TCTTATTCGTTGGAGACTTTATAACCCT	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11472_Mjap_Gib2022_COI	GLTA-085	1	BE11472_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	AATTTAATTGTGACTAGGCATGGTCTG	151.18227
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11473_Cmae_Bot2011_COI	GLTA-086	1	BE11473_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	ATGAACAGTCTATCCTCCTTTAG	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11473_Hsan_Wil2021_COI	GLTA-086	1	BE11473_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	CCTGGGCCGGTATAGTAGGT	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11473_Mros_Gib2022_COI	GLTA-086	1	BE11473_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11473_Msal_Bot2012_COI	GLTA-086	1	BE11473_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	GYTAGTTCCRATGATGTTAGCTG	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11473_Mstr_Wil2021_COI	GLTA-086	1	BE11473_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	GTAGTGGTAACTACTCATGCTTTA	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11473_Pvir_Dia2013_COI	GLTA-086	1	BE11473_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	CTTAGTGGCATTAATTCGDAATCC	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11473_Rhar_Sim2018_COI	GLTA-086	1	BE11473_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	CTGCTGCTATTGCTCATG	151.18417
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11475_Aamu_Bax2006_COI	GLTA-088	1	BE11475_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	GCACAACCGGGATCTTTACTTC	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11475_Pcan_Bot2011_IGS	GLTA-088	1	BE11475_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-088	IGS	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	CGTAATCCTCAGTACTGGCTA	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11475_Pper_Dia2013_COI	GLTA-088	1	BE11475_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	CTTAGTGGCATTAATTCGDAATCC	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11475_Sspa_Oph2007_COI	GLTA-088	1	BE11475_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	GAAGGCCTTCCGATGATC	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11475_Upin_Bot2011_COI	GLTA-088	1	BE11475_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	CTTTAATTACAGCGTTTTTATTGTTGT	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11475_Vgib_Wil2025_COI	GLTA-088	1	BE11475_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	CCCCTCTTTACAAGTTCTTTAGGGT	151.18114
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11476_Asen_Wil2025_COI	GLTA-089	1	BE11476_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	GTTGTTGTTACAACACATGCTCTTTTG	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11476_Cjap_Sim2018_COI	GLTA-089	1	BE11476_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11476_Esin_And2018_Cyb	GLTA-089	1	BE11476_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-089	Cytb	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	ACCCCTCCTCATATCCAACCA	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11476_Mgig_Bot2012_COI	GLTA-089	1	BE11476_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	TCTTATTCGTTGGAGACTTTATAACCCT	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11476_Mjap_Gib2022_COI	GLTA-089	1	BE11476_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	AATTTAATTGTGACTAGGCATGGTCTG	151.18258
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11477_Cmae_Bot2011_COI	GLTA-090	1	BE11477_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	ATGAACAGTCTATCCTCCTTTAG	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11477_Hsan_Wil2021_COI	GLTA-090	1	BE11477_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	CCTGGGCCGGTATAGTAGGT	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11477_Mros_Gib2022_COI	GLTA-090	1	BE11477_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11477_Msal_Bot2012_COI	GLTA-090	1	BE11477_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	GYTAGTTCCRATGATGTTAGCTG	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11477_Mstr_Wil2021_COI	GLTA-090	1	BE11477_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	GTAGTGGTAACTACTCATGCTTTA	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11477_Pvir_Dia2013_COI	GLTA-090	1	BE11477_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	CTTAGTGGCATTAATTCGDAATCC	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11477_Rhar_Sim2018_COI	GLTA-090	1	BE11477_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	CTGCTGCTATTGCTCATG	151.18425
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11479_Aamu_Bax2006_COI	GLTA-092	1	BE11479_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	GCACAACCGGGATCTTTACTTC	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11479_Pcan_Bot2011_IGS	GLTA-092	1	BE11479_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-092	IGS	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	CGTAATCCTCAGTACTGGCTA	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11479_Pper_Dia2013_COI	GLTA-092	1	BE11479_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	CTTAGTGGCATTAATTCGDAATCC	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11479_Sspa_Oph2007_COI	GLTA-092	1	BE11479_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	GAAGGCCTTCCGATGATC	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11479_Upin_Bot2011_COI	GLTA-092	1	BE11479_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	CTTTAATTACAGCGTTTTTATTGTTGT	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11479_Vgib_Wil2025_COI	GLTA-092	1	BE11479_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	CCCCTCTTTACAAGTTCTTTAGGGT	151.1796
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11480_Cmae_Bot2011_COI	GLTA-093	1	BE11480_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	ATGAACAGTCTATCCTCCTTTAG	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11480_Hsan_Wil2021_COI	GLTA-093	1	BE11480_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	CCTGGGCCGGTATAGTAGGT	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11480_Mros_Gib2022_COI	GLTA-093	1	BE11480_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11480_Msal_Bot2012_COI	GLTA-093	1	BE11480_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	GYTAGTTCCRATGATGTTAGCTG	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11480_Mstr_Wil2021_COI	GLTA-093	1	BE11480_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	GTAGTGGTAACTACTCATGCTTTA	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11480_Pvir_Dia2013_COI	GLTA-093	1	BE11480_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	CTTAGTGGCATTAATTCGDAATCC	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11480_Rhar_Sim2018_COI	GLTA-093	1	BE11480_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	CTGCTGCTATTGCTCATG	151.18133
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11482_Aamu_Bax2006_COI	GLTA-095	1	BE11482_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	GCACAACCGGGATCTTTACTTC	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11482_Pcan_Bot2011_IGS	GLTA-095	1	BE11482_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-095	IGS	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	CGTAATCCTCAGTACTGGCTA	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11482_Pper_Dia2013_COI	GLTA-095	1	BE11482_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	CTTAGTGGCATTAATTCGDAATCC	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11482_Sspa_Oph2007_COI	GLTA-095	1	BE11482_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	GAAGGCCTTCCGATGATC	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11482_Upin_Bot2011_COI	GLTA-095	1	BE11482_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	CTTTAATTACAGCGTTTTTATTGTTGT	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11482_Vgib_Wil2025_COI	GLTA-095	1	BE11482_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	CCCCTCTTTACAAGTTCTTTAGGGT	151.17867
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11483_Asen_Wil2025_COI	GLTA-096	1	BE11483_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	GTTGTTGTTACAACACATGCTCTTTTG	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11483_Cjap_Sim2018_COI	GLTA-096	1	BE11483_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11483_Esin_And2018_Cyb	GLTA-096	1	BE11483_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-096	Cytb	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	ACCCCTCCTCATATCCAACCA	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11483_Mgig_Bot2012_COI	GLTA-096	1	BE11483_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	TCTTATTCGTTGGAGACTTTATAACCCT	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11483_Mjap_Gib2022_COI	GLTA-096	1	BE11483_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	AATTTAATTGTGACTAGGCATGGTCTG	151.18079
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11484_Cmae_Bot2011_COI	GLTA-097	1	BE11484_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	ATGAACAGTCTATCCTCCTTTAG	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11484_Hsan_Wil2021_COI	GLTA-097	1	BE11484_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	CCTGGGCCGGTATAGTAGGT	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11484_Mros_Gib2022_COI	GLTA-097	1	BE11484_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11484_Msal_Bot2012_COI	GLTA-097	1	BE11484_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	GYTAGTTCCRATGATGTTAGCTG	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11484_Mstr_Wil2021_COI	GLTA-097	1	BE11484_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	GTAGTGGTAACTACTCATGCTTTA	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11484_Pvir_Dia2013_COI	GLTA-097	1	BE11484_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	CTTAGTGGCATTAATTCGDAATCC	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11484_Rhar_Sim2018_COI	GLTA-097	1	BE11484_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	CTGCTGCTATTGCTCATG	151.17661
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11486_Aamu_Bax2006_COI	GLTA-099	1	BE11486_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	GCACAACCGGGATCTTTACTTC	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11486_Pcan_Bot2011_IGS	GLTA-099	1	BE11486_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-099	IGS	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	CGTAATCCTCAGTACTGGCTA	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11486_Pper_Dia2013_COI	GLTA-099	1	BE11486_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	CTTAGTGGCATTAATTCGDAATCC	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11486_Sspa_Oph2007_COI	GLTA-099	1	BE11486_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	GAAGGCCTTCCGATGATC	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11486_Upin_Bot2011_COI	GLTA-099	1	BE11486_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	CTTTAATTACAGCGTTTTTATTGTTGT	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11486_Vgib_Wil2025_COI	GLTA-099	1	BE11486_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	CCCCTCTTTACAAGTTCTTTAGGGT	151.1738
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11487_Asen_Wil2025_COI	GLTA-100	1	BE11487_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	GTTGTTGTTACAACACATGCTCTTTTG	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11487_Cjap_Sim2018_COI	GLTA-100	1	BE11487_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11487_Esin_And2018_Cyb	GLTA-100	1	BE11487_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-100	Cytb	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	ACCCCTCCTCATATCCAACCA	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11487_Mgig_Bot2012_COI	GLTA-100	1	BE11487_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	TCTTATTCGTTGGAGACTTTATAACCCT	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11487_Mjap_Gib2022_COI	GLTA-100	1	BE11487_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	AATTTAATTGTGACTAGGCATGGTCTG	151.17533
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11488_Cmae_Bot2011_COI	GLTA-101	1	BE11488_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	ATGAACAGTCTATCCTCCTTTAG	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11488_Hsan_Wil2021_COI	GLTA-101	1	BE11488_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	CCTGGGCCGGTATAGTAGGT	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11488_Mros_Gib2022_COI	GLTA-101	1	BE11488_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11488_Msal_Bot2012_COI	GLTA-101	1	BE11488_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	GYTAGTTCCRATGATGTTAGCTG	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11488_Mstr_Wil2021_COI	GLTA-101	1	BE11488_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	GTAGTGGTAACTACTCATGCTTTA	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11488_Pvir_Dia2013_COI	GLTA-101	1	BE11488_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	CTTAGTGGCATTAATTCGDAATCC	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11488_Rhar_Sim2018_COI	GLTA-101	1	BE11488_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	CTGCTGCTATTGCTCATG	151.17702
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11490_Asen_Wil2025_COI	GLTA-103	1	BE11490_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	GTTGTTGTTACAACACATGCTCTTTTG	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11490_Cjap_Sim2018_COI	GLTA-103	1	BE11490_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11490_Esin_And2018_Cyb	GLTA-103	1	BE11490_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-103	Cytb	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	ACCCCTCCTCATATCCAACCA	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11490_Mgig_Bot2012_COI	GLTA-103	1	BE11490_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	TCTTATTCGTTGGAGACTTTATAACCCT	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11490_Mjap_Gib2022_COI	GLTA-103	1	BE11490_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	AATTTAATTGTGACTAGGCATGGTCTG	151.17436
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11491_Cmae_Bot2011_COI	GLTA-104	1	BE11491_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	ATGAACAGTCTATCCTCCTTTAG	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11491_Hsan_Wil2021_COI	GLTA-104	1	BE11491_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	CCTGGGCCGGTATAGTAGGT	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11491_Mros_Gib2022_COI	GLTA-104	1	BE11491_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11491_Msal_Bot2012_COI	GLTA-104	1	BE11491_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	GYTAGTTCCRATGATGTTAGCTG	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11491_Mstr_Wil2021_COI	GLTA-104	1	BE11491_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	GTAGTGGTAACTACTCATGCTTTA	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11491_Pvir_Dia2013_COI	GLTA-104	1	BE11491_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	CTTAGTGGCATTAATTCGDAATCC	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11491_Rhar_Sim2018_COI	GLTA-104	1	BE11491_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	CTGCTGCTATTGCTCATG	151.17602
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11497_Aamu_Bax2006_COI	GLTA-071	1	BE11497_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	GCACAACCGGGATCTTTACTTC	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11497_Pcan_Bot2011_IGS	GLTA-071	1	BE11497_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-071	IGS	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	CGTAATCCTCAGTACTGGCTA	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11497_Pper_Dia2013_COI	GLTA-071	1	BE11497_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	CTTAGTGGCATTAATTCGDAATCC	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11497_Sspa_Oph2007_COI	GLTA-071	1	BE11497_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	GAAGGCCTTCCGATGATC	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11497_Upin_Bot2011_COI	GLTA-071	1	BE11497_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	CTTTAATTACAGCGTTTTTATTGTTGT	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11497_Vgib_Wil2025_COI	GLTA-071	1	BE11497_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	CCCCTCTTTACAAGTTCTTTAGGGT	151.1845
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11498_Asen_Wil2025_COI	GLTA-072	1	BE11498_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	GTTGTTGTTACAACACATGCTCTTTTG	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11498_Cjap_Sim2018_COI	GLTA-072	1	BE11498_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11498_Esin_And2018_Cyb	GLTA-072	1	BE11498_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-072	Cytb	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	ACCCCTCCTCATATCCAACCA	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11498_Mgig_Bot2012_COI	GLTA-072	1	BE11498_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	TCTTATTCGTTGGAGACTTTATAACCCT	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11498_Mjap_Gib2022_COI	GLTA-072	1	BE11498_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	AATTTAATTGTGACTAGGCATGGTCTG	151.18449
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11499_Cmae_Bot2011_COI	GLTA-073	1	BE11499_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	ATGAACAGTCTATCCTCCTTTAG	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11499_Hsan_Wil2021_COI	GLTA-073	1	BE11499_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	CCTGGGCCGGTATAGTAGGT	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11499_Mros_Gib2022_COI	GLTA-073	1	BE11499_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11499_Msal_Bot2012_COI	GLTA-073	1	BE11499_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	GYTAGTTCCRATGATGTTAGCTG	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11499_Mstr_Wil2021_COI	GLTA-073	1	BE11499_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	GTAGTGGTAACTACTCATGCTTTA	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11499_Pvir_Dia2013_COI	GLTA-073	1	BE11499_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	CTTAGTGGCATTAATTCGDAATCC	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11499_Rhar_Sim2018_COI	GLTA-073	1	BE11499_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	CTGCTGCTATTGCTCATG	151.18617
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11501_Aamu_Bax2006_COI	GLTA-075	1	BE11501_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	GCACAACCGGGATCTTTACTTC	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11501_Pcan_Bot2011_IGS	GLTA-075	1	BE11501_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-075	IGS	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	CGTAATCCTCAGTACTGGCTA	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11501_Pper_Dia2013_COI	GLTA-075	1	BE11501_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	CTTAGTGGCATTAATTCGDAATCC	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11501_Sspa_Oph2007_COI	GLTA-075	1	BE11501_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	GAAGGCCTTCCGATGATC	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11501_Upin_Bot2011_COI	GLTA-075	1	BE11501_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	CTTTAATTACAGCGTTTTTATTGTTGT	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11501_Vgib_Wil2025_COI	GLTA-075	1	BE11501_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	CCCCTCTTTACAAGTTCTTTAGGGT	151.1821
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11502_Asen_Wil2025_COI	DRWA-001	1	BE11502_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	GTTGTTGTTACAACACATGCTCTTTTG	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11502_Cjap_Sim2018_COI	DRWA-001	1	BE11502_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11502_Esin_And2018_Cyb	DRWA-001	1	BE11502_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-001	Cytb	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	ACCCCTCCTCATATCCAACCA	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11502_Mgig_Bot2012_COI	DRWA-001	1	BE11502_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	TCTTATTCGTTGGAGACTTTATAACCCT	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11502_Mjap_Gib2022_COI	DRWA-001	1	BE11502_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	AATTTAATTGTGACTAGGCATGGTCTG	130.84747
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11503_Cmae_Bot2011_COI	DRWA-002	1	BE11503_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	ATGAACAGTCTATCCTCCTTTAG	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11503_Hsan_Wil2021_COI	DRWA-002	1	BE11503_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	CCTGGGCCGGTATAGTAGGT	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11503_Mros_Gib2022_COI	DRWA-002	1	BE11503_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11503_Msal_Bot2012_COI	DRWA-002	1	BE11503_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	GYTAGTTCCRATGATGTTAGCTG	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11503_Mstr_Wil2021_COI	DRWA-002	1	BE11503_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	GTAGTGGTAACTACTCATGCTTTA	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11503_Pvir_Dia2013_COI	DRWA-002	1	BE11503_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	CTTAGTGGCATTAATTCGDAATCC	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11503_Rhar_Sim2018_COI	DRWA-002	1	BE11503_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	CTGCTGCTATTGCTCATG	130.84915
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11505_Aamu_Bax2006_COI	DRWA-004	1	BE11505_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	GCACAACCGGGATCTTTACTTC	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11505_Pcan_Bot2011_IGS	DRWA-004	1	BE11505_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-004	IGS	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	CGTAATCCTCAGTACTGGCTA	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11505_Pper_Dia2013_COI	DRWA-004	1	BE11505_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	CTTAGTGGCATTAATTCGDAATCC	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11505_Sspa_Oph2007_COI	DRWA-004	1	BE11505_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	GAAGGCCTTCCGATGATC	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11505_Upin_Bot2011_COI	DRWA-004	1	BE11505_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	CTTTAATTACAGCGTTTTTATTGTTGT	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11505_Vgib_Wil2025_COI	DRWA-004	1	BE11505_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	CCCCTCTTTACAAGTTCTTTAGGGT	130.852
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11506_Asen_Wil2025_COI	DRWA-005	1	BE11506_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	GTTGTTGTTACAACACATGCTCTTTTG	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11506_Cjap_Sim2018_COI	DRWA-005	1	BE11506_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11506_Esin_And2018_Cyb	DRWA-005	1	BE11506_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-005	Cytb	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	ACCCCTCCTCATATCCAACCA	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11506_Mgig_Bot2012_COI	DRWA-005	1	BE11506_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	TCTTATTCGTTGGAGACTTTATAACCCT	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11506_Mjap_Gib2022_COI	DRWA-005	1	BE11506_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	AATTTAATTGTGACTAGGCATGGTCTG	130.85324
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11507_Cmae_Bot2011_COI	DRWA-006	1	BE11507_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	ATGAACAGTCTATCCTCCTTTAG	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11507_Hsan_Wil2021_COI	DRWA-006	1	BE11507_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	CCTGGGCCGGTATAGTAGGT	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11507_Mros_Gib2022_COI	DRWA-006	1	BE11507_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11507_Msal_Bot2012_COI	DRWA-006	1	BE11507_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	GYTAGTTCCRATGATGTTAGCTG	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11507_Mstr_Wil2021_COI	DRWA-006	1	BE11507_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	GTAGTGGTAACTACTCATGCTTTA	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11507_Pvir_Dia2013_COI	DRWA-006	1	BE11507_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	CTTAGTGGCATTAATTCGDAATCC	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11507_Rhar_Sim2018_COI	DRWA-006	1	BE11507_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	CTGCTGCTATTGCTCATG	130.84666
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11509_Aamu_Bax2006_COI	DRWA-008	1	BE11509_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	GCACAACCGGGATCTTTACTTC	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11509_Pcan_Bot2011_IGS	DRWA-008	1	BE11509_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-008	IGS	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	CGTAATCCTCAGTACTGGCTA	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11509_Pper_Dia2013_COI	DRWA-008	1	BE11509_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	CTTAGTGGCATTAATTCGDAATCC	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11509_Sspa_Oph2007_COI	DRWA-008	1	BE11509_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	GAAGGCCTTCCGATGATC	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11509_Upin_Bot2011_COI	DRWA-008	1	BE11509_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	CTTTAATTACAGCGTTTTTATTGTTGT	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11509_Vgib_Wil2025_COI	DRWA-008	1	BE11509_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	CCCCTCTTTACAAGTTCTTTAGGGT	130.84983
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11510_Cmae_Bot2011_COI	DRWA-009	1	BE11510_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	ATGAACAGTCTATCCTCCTTTAG	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11510_Hsan_Wil2021_COI	DRWA-009	1	BE11510_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	CCTGGGCCGGTATAGTAGGT	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11510_Mros_Gib2022_COI	DRWA-009	1	BE11510_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11510_Msal_Bot2012_COI	DRWA-009	1	BE11510_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	GYTAGTTCCRATGATGTTAGCTG	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11510_Mstr_Wil2021_COI	DRWA-009	1	BE11510_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	GTAGTGGTAACTACTCATGCTTTA	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11510_Pvir_Dia2013_COI	DRWA-009	1	BE11510_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	CTTAGTGGCATTAATTCGDAATCC	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11510_Rhar_Sim2018_COI	DRWA-009	1	BE11510_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	CTGCTGCTATTGCTCATG	130.85118
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11512_Aamu_Bax2006_COI	DRWA-011	1	BE11512_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	GCACAACCGGGATCTTTACTTC	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11512_Pcan_Bot2011_IGS	DRWA-011	1	BE11512_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-011	IGS	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	CGTAATCCTCAGTACTGGCTA	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11512_Pper_Dia2013_COI	DRWA-011	1	BE11512_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	CTTAGTGGCATTAATTCGDAATCC	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11512_Sspa_Oph2007_COI	DRWA-011	1	BE11512_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	GAAGGCCTTCCGATGATC	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11512_Upin_Bot2011_COI	DRWA-011	1	BE11512_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	CTTTAATTACAGCGTTTTTATTGTTGT	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11512_Vgib_Wil2025_COI	DRWA-011	1	BE11512_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	CCCCTCTTTACAAGTTCTTTAGGGT	130.85392
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11513_Asen_Wil2025_COI	DRWA-012	1	BE11513_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	GTTGTTGTTACAACACATGCTCTTTTG	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11513_Cjap_Sim2018_COI	DRWA-012	1	BE11513_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11513_Esin_And2018_Cyb	DRWA-012	1	BE11513_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-012	Cytb	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	ACCCCTCCTCATATCCAACCA	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11513_Mgig_Bot2012_COI	DRWA-012	1	BE11513_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	TCTTATTCGTTGGAGACTTTATAACCCT	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11513_Mjap_Gib2022_COI	DRWA-012	1	BE11513_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	AATTTAATTGTGACTAGGCATGGTCTG	130.84598
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11514_Cmae_Bot2011_COI	DRWA-013	1	BE11514_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	ATGAACAGTCTATCCTCCTTTAG	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11514_Hsan_Wil2021_COI	DRWA-013	1	BE11514_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	CCTGGGCCGGTATAGTAGGT	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11514_Mros_Gib2022_COI	DRWA-013	1	BE11514_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11514_Msal_Bot2012_COI	DRWA-013	1	BE11514_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	GYTAGTTCCRATGATGTTAGCTG	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11514_Mstr_Wil2021_COI	DRWA-013	1	BE11514_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	GTAGTGGTAACTACTCATGCTTTA	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11514_Pvir_Dia2013_COI	DRWA-013	1	BE11514_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	CTTAGTGGCATTAATTCGDAATCC	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11514_Rhar_Sim2018_COI	DRWA-013	1	BE11514_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	CTGCTGCTATTGCTCATG	130.84737
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11516_Aamu_Bax2006_COI	DRWA-015	1	BE11516_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	GCACAACCGGGATCTTTACTTC	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11516_Pcan_Bot2011_IGS	DRWA-015	1	BE11516_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-015	IGS	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	CGTAATCCTCAGTACTGGCTA	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11516_Pper_Dia2013_COI	DRWA-015	1	BE11516_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	CTTAGTGGCATTAATTCGDAATCC	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11516_Sspa_Oph2007_COI	DRWA-015	1	BE11516_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	GAAGGCCTTCCGATGATC	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11516_Upin_Bot2011_COI	DRWA-015	1	BE11516_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	CTTTAATTACAGCGTTTTTATTGTTGT	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11516_Vgib_Wil2025_COI	DRWA-015	1	BE11516_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	CCCCTCTTTACAAGTTCTTTAGGGT	130.84672
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11517_Asen_Wil2025_COI	DRWA-016	1	BE11517_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	GTTGTTGTTACAACACATGCTCTTTTG	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11517_Cjap_Sim2018_COI	DRWA-016	1	BE11517_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11517_Esin_And2018_Cyb	DRWA-016	1	BE11517_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-016	Cytb	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	ACCCCTCCTCATATCCAACCA	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11517_Mgig_Bot2012_COI	DRWA-016	1	BE11517_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	TCTTATTCGTTGGAGACTTTATAACCCT	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11517_Mjap_Gib2022_COI	DRWA-016	1	BE11517_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	AATTTAATTGTGACTAGGCATGGTCTG	130.84482
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11518_Cmae_Bot2011_COI	DRWA-017	1	BE11518_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	ATGAACAGTCTATCCTCCTTTAG	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11518_Hsan_Wil2021_COI	DRWA-017	1	BE11518_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	CCTGGGCCGGTATAGTAGGT	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11518_Mros_Gib2022_COI	DRWA-017	1	BE11518_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11518_Msal_Bot2012_COI	DRWA-017	1	BE11518_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	GYTAGTTCCRATGATGTTAGCTG	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11518_Mstr_Wil2021_COI	DRWA-017	1	BE11518_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	GTAGTGGTAACTACTCATGCTTTA	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11518_Pvir_Dia2013_COI	DRWA-017	1	BE11518_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	CTTAGTGGCATTAATTCGDAATCC	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11518_Rhar_Sim2018_COI	DRWA-017	1	BE11518_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	CTGCTGCTATTGCTCATG	130.846
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11520_Asen_Wil2025_COI	DRWA-019	1	BE11520_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	GTTGTTGTTACAACACATGCTCTTTTG	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11520_Cjap_Sim2018_COI	DRWA-019	1	BE11520_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11520_Esin_And2018_Cyb	DRWA-019	1	BE11520_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-019	Cytb	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	ACCCCTCCTCATATCCAACCA	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11520_Mgig_Bot2012_COI	DRWA-019	1	BE11520_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	TCTTATTCGTTGGAGACTTTATAACCCT	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11520_Mjap_Gib2022_COI	DRWA-019	1	BE11520_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	AATTTAATTGTGACTAGGCATGGTCTG	130.84673
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11521_Cmae_Bot2011_COI	DRWA-020	1	BE11521_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	ATGAACAGTCTATCCTCCTTTAG	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11521_Hsan_Wil2021_COI	DRWA-020	1	BE11521_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	CCTGGGCCGGTATAGTAGGT	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11521_Mros_Gib2022_COI	DRWA-020	1	BE11521_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11521_Msal_Bot2012_COI	DRWA-020	1	BE11521_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	GYTAGTTCCRATGATGTTAGCTG	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11521_Mstr_Wil2021_COI	DRWA-020	1	BE11521_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	GTAGTGGTAACTACTCATGCTTTA	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11521_Pvir_Dia2013_COI	DRWA-020	1	BE11521_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	CTTAGTGGCATTAATTCGDAATCC	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11521_Rhar_Sim2018_COI	DRWA-020	1	BE11521_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	CTGCTGCTATTGCTCATG	130.84479
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11523_Aamu_Bax2006_COI	DRWA-022	1	BE11523_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	GCACAACCGGGATCTTTACTTC	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11523_Pcan_Bot2011_IGS	DRWA-022	1	BE11523_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-022	IGS	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	CGTAATCCTCAGTACTGGCTA	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11523_Pper_Dia2013_COI	DRWA-022	1	BE11523_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	CTTAGTGGCATTAATTCGDAATCC	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11523_Sspa_Oph2007_COI	DRWA-022	1	BE11523_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	GAAGGCCTTCCGATGATC	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11523_Upin_Bot2011_COI	DRWA-022	1	BE11523_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	CTTTAATTACAGCGTTTTTATTGTTGT	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11523_Vgib_Wil2025_COI	DRWA-022	1	BE11523_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	CCCCTCTTTACAAGTTCTTTAGGGT	130.84736
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11524_Asen_Wil2025_COI	DRWA-023	1	BE11524_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	GTTGTTGTTACAACACATGCTCTTTTG	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11524_Cjap_Sim2018_COI	DRWA-023	1	BE11524_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11524_Esin_And2018_Cyb	DRWA-023	1	BE11524_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-023	Cytb	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	ACCCCTCCTCATATCCAACCA	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11524_Mgig_Bot2012_COI	DRWA-023	1	BE11524_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	TCTTATTCGTTGGAGACTTTATAACCCT	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11524_Mjap_Gib2022_COI	DRWA-023	1	BE11524_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	AATTTAATTGTGACTAGGCATGGTCTG	130.84894
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11525_Cmae_Bot2011_COI	DRWA-024	1	BE11525_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	ATGAACAGTCTATCCTCCTTTAG	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11525_Hsan_Wil2021_COI	DRWA-024	1	BE11525_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	CCTGGGCCGGTATAGTAGGT	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11525_Mros_Gib2022_COI	DRWA-024	1	BE11525_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11525_Msal_Bot2012_COI	DRWA-024	1	BE11525_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	GYTAGTTCCRATGATGTTAGCTG	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11525_Mstr_Wil2021_COI	DRWA-024	1	BE11525_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	GTAGTGGTAACTACTCATGCTTTA	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11525_Pvir_Dia2013_COI	DRWA-024	1	BE11525_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	CTTAGTGGCATTAATTCGDAATCC	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11525_Rhar_Sim2018_COI	DRWA-024	1	BE11525_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	CTGCTGCTATTGCTCATG	130.85033
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11527_Aamu_Bax2006_COI	DRWA-026	1	BE11527_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	GCACAACCGGGATCTTTACTTC	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11527_Pcan_Bot2011_IGS	DRWA-026	1	BE11527_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-026	IGS	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	CGTAATCCTCAGTACTGGCTA	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11527_Pper_Dia2013_COI	DRWA-026	1	BE11527_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	CTTAGTGGCATTAATTCGDAATCC	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11527_Sspa_Oph2007_COI	DRWA-026	1	BE11527_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	GAAGGCCTTCCGATGATC	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11527_Upin_Bot2011_COI	DRWA-026	1	BE11527_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	CTTTAATTACAGCGTTTTTATTGTTGT	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11527_Vgib_Wil2025_COI	DRWA-026	1	BE11527_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	CCCCTCTTTACAAGTTCTTTAGGGT	130.84675
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11528_Asen_Wil2025_COI	DRWA-027	1	BE11528_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	GTTGTTGTTACAACACATGCTCTTTTG	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11528_Cjap_Sim2018_COI	DRWA-027	1	BE11528_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11528_Esin_And2018_Cyb	DRWA-027	1	BE11528_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-027	Cytb	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	ACCCCTCCTCATATCCAACCA	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11528_Mgig_Bot2012_COI	DRWA-027	1	BE11528_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	TCTTATTCGTTGGAGACTTTATAACCCT	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11528_Mjap_Gib2022_COI	DRWA-027	1	BE11528_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	AATTTAATTGTGACTAGGCATGGTCTG	130.848
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11529_Cmae_Bot2011_COI	DRWA-028	1	BE11529_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	ATGAACAGTCTATCCTCCTTTAG	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11529_Hsan_Wil2021_COI	DRWA-028	1	BE11529_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	CCTGGGCCGGTATAGTAGGT	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11529_Mros_Gib2022_COI	DRWA-028	1	BE11529_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11529_Msal_Bot2012_COI	DRWA-028	1	BE11529_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	GYTAGTTCCRATGATGTTAGCTG	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11529_Mstr_Wil2021_COI	DRWA-028	1	BE11529_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	GTAGTGGTAACTACTCATGCTTTA	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11529_Pvir_Dia2013_COI	DRWA-028	1	BE11529_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	CTTAGTGGCATTAATTCGDAATCC	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11529_Rhar_Sim2018_COI	DRWA-028	1	BE11529_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	CTGCTGCTATTGCTCATG	130.84944
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11530_Aamu_Bax2006_COI	DRWA-029	1	BE11530_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	GCACAACCGGGATCTTTACTTC	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11530_Pcan_Bot2011_IGS	DRWA-029	1	BE11530_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-029	IGS	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	CGTAATCCTCAGTACTGGCTA	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11530_Pper_Dia2013_COI	DRWA-029	1	BE11530_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	CTTAGTGGCATTAATTCGDAATCC	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11530_Sspa_Oph2007_COI	DRWA-029	1	BE11530_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	GAAGGCCTTCCGATGATC	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11530_Upin_Bot2011_COI	DRWA-029	1	BE11530_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	CTTTAATTACAGCGTTTTTATTGTTGT	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11530_Vgib_Wil2025_COI	DRWA-029	1	BE11530_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	CCCCTCTTTACAAGTTCTTTAGGGT	130.84747
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11531_Asen_Wil2025_COI	DRWA-030	1	BE11531_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	GTTGTTGTTACAACACATGCTCTTTTG	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11531_Cjap_Sim2018_COI	DRWA-030	1	BE11531_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11531_Esin_And2018_Cyb	DRWA-030	1	BE11531_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-030	Cytb	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	ACCCCTCCTCATATCCAACCA	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11531_Mgig_Bot2012_COI	DRWA-030	1	BE11531_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	TCTTATTCGTTGGAGACTTTATAACCCT	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11531_Mjap_Gib2022_COI	DRWA-030	1	BE11531_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	AATTTAATTGTGACTAGGCATGGTCTG	130.84867
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11532_Cmae_Bot2011_COI	DRWA-031	1	BE11532_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	ATGAACAGTCTATCCTCCTTTAG	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11532_Hsan_Wil2021_COI	DRWA-031	1	BE11532_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	CCTGGGCCGGTATAGTAGGT	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11532_Mros_Gib2022_COI	DRWA-031	1	BE11532_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11532_Msal_Bot2012_COI	DRWA-031	1	BE11532_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	GYTAGTTCCRATGATGTTAGCTG	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11532_Mstr_Wil2021_COI	DRWA-031	1	BE11532_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	GTAGTGGTAACTACTCATGCTTTA	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11532_Pvir_Dia2013_COI	DRWA-031	1	BE11532_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	CTTAGTGGCATTAATTCGDAATCC	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11532_Rhar_Sim2018_COI	DRWA-031	1	BE11532_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	CTGCTGCTATTGCTCATG	130.85026
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11534_Aamu_Bax2006_COI	DRWA-033	1	BE11534_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	GCACAACCGGGATCTTTACTTC	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11534_Pcan_Bot2011_IGS	DRWA-033	1	BE11534_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-033	IGS	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	CGTAATCCTCAGTACTGGCTA	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11534_Pper_Dia2013_COI	DRWA-033	1	BE11534_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	CTTAGTGGCATTAATTCGDAATCC	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11534_Sspa_Oph2007_COI	DRWA-033	1	BE11534_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	GAAGGCCTTCCGATGATC	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11534_Upin_Bot2011_COI	DRWA-033	1	BE11534_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	CTTTAATTACAGCGTTTTTATTGTTGT	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11534_Vgib_Wil2025_COI	DRWA-033	1	BE11534_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	CCCCTCTTTACAAGTTCTTTAGGGT	130.84738
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11535_Asen_Wil2025_COI	DRWA-034	1	BE11535_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	GTTGTTGTTACAACACATGCTCTTTTG	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11535_Cjap_Sim2018_COI	DRWA-034	1	BE11535_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11535_Esin_And2018_Cyb	DRWA-034	1	BE11535_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-034	Cytb	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	ACCCCTCCTCATATCCAACCA	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11535_Mgig_Bot2012_COI	DRWA-034	1	BE11535_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	TCTTATTCGTTGGAGACTTTATAACCCT	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11535_Mjap_Gib2022_COI	DRWA-034	1	BE11535_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	AATTTAATTGTGACTAGGCATGGTCTG	130.84775
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11536_Cmae_Bot2011_COI	DRWA-035	1	BE11536_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	ATGAACAGTCTATCCTCCTTTAG	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11536_Hsan_Wil2021_COI	DRWA-035	1	BE11536_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	CCTGGGCCGGTATAGTAGGT	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11536_Mros_Gib2022_COI	DRWA-035	1	BE11536_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11536_Msal_Bot2012_COI	DRWA-035	1	BE11536_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	GYTAGTTCCRATGATGTTAGCTG	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11536_Mstr_Wil2021_COI	DRWA-035	1	BE11536_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	GTAGTGGTAACTACTCATGCTTTA	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11536_Pvir_Dia2013_COI	DRWA-035	1	BE11536_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	CTTAGTGGCATTAATTCGDAATCC	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11536_Rhar_Sim2018_COI	DRWA-035	1	BE11536_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	CTGCTGCTATTGCTCATG	130.85165
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11541_Aamu_Bax2006_COI	DRWA-106	1	BE11541_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	GCACAACCGGGATCTTTACTTC	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11541_Pcan_Bot2011_IGS	DRWA-106	1	BE11541_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-106	IGS	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	CGTAATCCTCAGTACTGGCTA	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11541_Pper_Dia2013_COI	DRWA-106	1	BE11541_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	CTTAGTGGCATTAATTCGDAATCC	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11541_Sspa_Oph2007_COI	DRWA-106	1	BE11541_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	GAAGGCCTTCCGATGATC	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11541_Upin_Bot2011_COI	DRWA-106	1	BE11541_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	CTTTAATTACAGCGTTTTTATTGTTGT	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11541_Vgib_Wil2025_COI	DRWA-106	1	BE11541_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	CCCCTCTTTACAAGTTCTTTAGGGT	130.90486
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11542_Asen_Wil2025_COI	DRWA-107	1	BE11542_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	GTTGTTGTTACAACACATGCTCTTTTG	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11542_Cjap_Sim2018_COI	DRWA-107	1	BE11542_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11542_Esin_And2018_Cyb	DRWA-107	1	BE11542_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-107	Cytb	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	ACCCCTCCTCATATCCAACCA	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11542_Mgig_Bot2012_COI	DRWA-107	1	BE11542_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	TCTTATTCGTTGGAGACTTTATAACCCT	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11542_Mjap_Gib2022_COI	DRWA-107	1	BE11542_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	AATTTAATTGTGACTAGGCATGGTCTG	130.9044
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11543_Cmae_Bot2011_COI	DRWA-108	1	BE11543_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	ATGAACAGTCTATCCTCCTTTAG	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11543_Hsan_Wil2021_COI	DRWA-108	1	BE11543_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	CCTGGGCCGGTATAGTAGGT	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11543_Mros_Gib2022_COI	DRWA-108	1	BE11543_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11543_Msal_Bot2012_COI	DRWA-108	1	BE11543_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	GYTAGTTCCRATGATGTTAGCTG	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11543_Mstr_Wil2021_COI	DRWA-108	1	BE11543_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	GTAGTGGTAACTACTCATGCTTTA	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11543_Pvir_Dia2013_COI	DRWA-108	1	BE11543_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	CTTAGTGGCATTAATTCGDAATCC	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11543_Rhar_Sim2018_COI	DRWA-108	1	BE11543_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	CTGCTGCTATTGCTCATG	130.90387
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11545_Aamu_Bax2006_COI	DRWA-110	1	BE11545_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	GCACAACCGGGATCTTTACTTC	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11545_Pcan_Bot2011_IGS	DRWA-110	1	BE11545_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-110	IGS	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	CGTAATCCTCAGTACTGGCTA	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11545_Pper_Dia2013_COI	DRWA-110	1	BE11545_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	CTTAGTGGCATTAATTCGDAATCC	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11545_Sspa_Oph2007_COI	DRWA-110	1	BE11545_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	GAAGGCCTTCCGATGATC	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11545_Upin_Bot2011_COI	DRWA-110	1	BE11545_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	CTTTAATTACAGCGTTTTTATTGTTGT	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11545_Vgib_Wil2025_COI	DRWA-110	1	BE11545_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	CCCCTCTTTACAAGTTCTTTAGGGT	130.90553
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11546_Asen_Wil2025_COI	DRWA-111	1	BE11546_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	GTTGTTGTTACAACACATGCTCTTTTG	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11546_Cjap_Sim2018_COI	DRWA-111	1	BE11546_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11546_Esin_And2018_Cyb	DRWA-111	1	BE11546_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-111	Cytb	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	ACCCCTCCTCATATCCAACCA	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11546_Mgig_Bot2012_COI	DRWA-111	1	BE11546_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	TCTTATTCGTTGGAGACTTTATAACCCT	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11546_Mjap_Gib2022_COI	DRWA-111	1	BE11546_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	AATTTAATTGTGACTAGGCATGGTCTG	130.90358
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11547_Cmae_Bot2011_COI	DRWA-112	1	BE11547_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	ATGAACAGTCTATCCTCCTTTAG	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11547_Hsan_Wil2021_COI	DRWA-112	1	BE11547_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	CCTGGGCCGGTATAGTAGGT	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11547_Mros_Gib2022_COI	DRWA-112	1	BE11547_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11547_Msal_Bot2012_COI	DRWA-112	1	BE11547_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	GYTAGTTCCRATGATGTTAGCTG	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11547_Mstr_Wil2021_COI	DRWA-112	1	BE11547_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	GTAGTGGTAACTACTCATGCTTTA	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11547_Pvir_Dia2013_COI	DRWA-112	1	BE11547_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	CTTAGTGGCATTAATTCGDAATCC	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11547_Rhar_Sim2018_COI	DRWA-112	1	BE11547_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	CTGCTGCTATTGCTCATG	130.90503
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11549_Aamu_Bax2006_COI	DRWA-114	1	BE11549_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	GCACAACCGGGATCTTTACTTC	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11549_Pcan_Bot2011_IGS	DRWA-114	1	BE11549_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-114	IGS	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	CGTAATCCTCAGTACTGGCTA	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11549_Pper_Dia2013_COI	DRWA-114	1	BE11549_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	CTTAGTGGCATTAATTCGDAATCC	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11549_Sspa_Oph2007_COI	DRWA-114	1	BE11549_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	GAAGGCCTTCCGATGATC	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11549_Upin_Bot2011_COI	DRWA-114	1	BE11549_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	CTTTAATTACAGCGTTTTTATTGTTGT	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11549_Vgib_Wil2025_COI	DRWA-114	1	BE11549_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	CCCCTCTTTACAAGTTCTTTAGGGT	130.90575
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11550_Cmae_Bot2011_COI	DRWA-115	1	BE11550_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	ATGAACAGTCTATCCTCCTTTAG	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11550_Hsan_Wil2021_COI	DRWA-115	1	BE11550_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	CCTGGGCCGGTATAGTAGGT	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11550_Mros_Gib2022_COI	DRWA-115	1	BE11550_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11550_Msal_Bot2012_COI	DRWA-115	1	BE11550_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	GYTAGTTCCRATGATGTTAGCTG	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11550_Mstr_Wil2021_COI	DRWA-115	1	BE11550_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	GTAGTGGTAACTACTCATGCTTTA	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11550_Pvir_Dia2013_COI	DRWA-115	1	BE11550_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	CTTAGTGGCATTAATTCGDAATCC	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11550_Rhar_Sim2018_COI	DRWA-115	1	BE11550_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	CTGCTGCTATTGCTCATG	130.90702
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11552_Aamu_Bax2006_COI	DRWA-117	1	BE11552_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	GCACAACCGGGATCTTTACTTC	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11552_Pcan_Bot2011_IGS	DRWA-117	1	BE11552_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-117	IGS	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	CGTAATCCTCAGTACTGGCTA	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11552_Pper_Dia2013_COI	DRWA-117	1	BE11552_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	CTTAGTGGCATTAATTCGDAATCC	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11552_Sspa_Oph2007_COI	DRWA-117	1	BE11552_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	GAAGGCCTTCCGATGATC	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11552_Upin_Bot2011_COI	DRWA-117	1	BE11552_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	CTTTAATTACAGCGTTTTTATTGTTGT	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11552_Vgib_Wil2025_COI	DRWA-117	1	BE11552_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	CCCCTCTTTACAAGTTCTTTAGGGT	130.90413
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11553_Asen_Wil2025_COI	DRWA-118	1	BE11553_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	GTTGTTGTTACAACACATGCTCTTTTG	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11553_Cjap_Sim2018_COI	DRWA-118	1	BE11553_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11553_Esin_And2018_Cyb	DRWA-118	1	BE11553_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-118	Cytb	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	ACCCCTCCTCATATCCAACCA	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11553_Mgig_Bot2012_COI	DRWA-118	1	BE11553_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	TCTTATTCGTTGGAGACTTTATAACCCT	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11553_Mjap_Gib2022_COI	DRWA-118	1	BE11553_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	AATTTAATTGTGACTAGGCATGGTCTG	130.90494
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11554_Cmae_Bot2011_COI	DRWA-119	1	BE11554_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	ATGAACAGTCTATCCTCCTTTAG	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11554_Hsan_Wil2021_COI	DRWA-119	1	BE11554_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	CCTGGGCCGGTATAGTAGGT	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11554_Mros_Gib2022_COI	DRWA-119	1	BE11554_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11554_Msal_Bot2012_COI	DRWA-119	1	BE11554_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	GYTAGTTCCRATGATGTTAGCTG	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11554_Mstr_Wil2021_COI	DRWA-119	1	BE11554_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	GTAGTGGTAACTACTCATGCTTTA	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11554_Pvir_Dia2013_COI	DRWA-119	1	BE11554_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	CTTAGTGGCATTAATTCGDAATCC	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11554_Rhar_Sim2018_COI	DRWA-119	1	BE11554_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	CTGCTGCTATTGCTCATG	130.90649
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11556_Aamu_Bax2006_COI	DRWA-121	1	BE11556_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	GCACAACCGGGATCTTTACTTC	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11556_Pcan_Bot2011_IGS	DRWA-121	1	BE11556_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-121	IGS	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	CGTAATCCTCAGTACTGGCTA	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11556_Pper_Dia2013_COI	DRWA-121	1	BE11556_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	CTTAGTGGCATTAATTCGDAATCC	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11556_Sspa_Oph2007_COI	DRWA-121	1	BE11556_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	GAAGGCCTTCCGATGATC	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11556_Upin_Bot2011_COI	DRWA-121	1	BE11556_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	CTTTAATTACAGCGTTTTTATTGTTGT	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11556_Vgib_Wil2025_COI	DRWA-121	1	BE11556_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	CCCCTCTTTACAAGTTCTTTAGGGT	130.90885
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11557_Asen_Wil2025_COI	DRWA-122	1	BE11557_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	GTTGTTGTTACAACACATGCTCTTTTG	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11557_Cjap_Sim2018_COI	DRWA-122	1	BE11557_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11557_Esin_And2018_Cyb	DRWA-122	1	BE11557_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-122	Cytb	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	ACCCCTCCTCATATCCAACCA	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11557_Mgig_Bot2012_COI	DRWA-122	1	BE11557_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	TCTTATTCGTTGGAGACTTTATAACCCT	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11557_Mjap_Gib2022_COI	DRWA-122	1	BE11557_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	AATTTAATTGTGACTAGGCATGGTCTG	130.91004
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11558_Cmae_Bot2011_COI	DRWA-123	1	BE11558_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	ATGAACAGTCTATCCTCCTTTAG	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11558_Hsan_Wil2021_COI	DRWA-123	1	BE11558_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	CCTGGGCCGGTATAGTAGGT	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11558_Mros_Gib2022_COI	DRWA-123	1	BE11558_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11558_Msal_Bot2012_COI	DRWA-123	1	BE11558_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	GYTAGTTCCRATGATGTTAGCTG	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11558_Mstr_Wil2021_COI	DRWA-123	1	BE11558_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	GTAGTGGTAACTACTCATGCTTTA	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11558_Pvir_Dia2013_COI	DRWA-123	1	BE11558_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	CTTAGTGGCATTAATTCGDAATCC	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11558_Rhar_Sim2018_COI	DRWA-123	1	BE11558_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	CTGCTGCTATTGCTCATG	130.9111
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11560_Asen_Wil2025_COI	DRWA-125	1	BE11560_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	GTTGTTGTTACAACACATGCTCTTTTG	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11560_Cjap_Sim2018_COI	DRWA-125	1	BE11560_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11560_Esin_And2018_Cyb	DRWA-125	1	BE11560_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-125	Cytb	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	ACCCCTCCTCATATCCAACCA	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11560_Mgig_Bot2012_COI	DRWA-125	1	BE11560_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	TCTTATTCGTTGGAGACTTTATAACCCT	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11560_Mjap_Gib2022_COI	DRWA-125	1	BE11560_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	AATTTAATTGTGACTAGGCATGGTCTG	130.91337
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11561_Cmae_Bot2011_COI	DRWA-126	1	BE11561_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	ATGAACAGTCTATCCTCCTTTAG	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11561_Hsan_Wil2021_COI	DRWA-126	1	BE11561_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	CCTGGGCCGGTATAGTAGGT	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11561_Mros_Gib2022_COI	DRWA-126	1	BE11561_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11561_Msal_Bot2012_COI	DRWA-126	1	BE11561_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	GYTAGTTCCRATGATGTTAGCTG	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11561_Mstr_Wil2021_COI	DRWA-126	1	BE11561_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	GTAGTGGTAACTACTCATGCTTTA	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11561_Pvir_Dia2013_COI	DRWA-126	1	BE11561_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	CTTAGTGGCATTAATTCGDAATCC	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11561_Rhar_Sim2018_COI	DRWA-126	1	BE11561_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	CTGCTGCTATTGCTCATG	130.90563
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11563_Aamu_Bax2006_COI	DRWA-128	1	BE11563_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	GCACAACCGGGATCTTTACTTC	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11563_Pcan_Bot2011_IGS	DRWA-128	1	BE11563_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-128	IGS	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	CGTAATCCTCAGTACTGGCTA	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11563_Pper_Dia2013_COI	DRWA-128	1	BE11563_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	CTTAGTGGCATTAATTCGDAATCC	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11563_Sspa_Oph2007_COI	DRWA-128	1	BE11563_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	GAAGGCCTTCCGATGATC	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11563_Upin_Bot2011_COI	DRWA-128	1	BE11563_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	CTTTAATTACAGCGTTTTTATTGTTGT	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11563_Vgib_Wil2025_COI	DRWA-128	1	BE11563_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	CCCCTCTTTACAAGTTCTTTAGGGT	130.90833
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11564_Asen_Wil2025_COI	DRWA-129	1	BE11564_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	GTTGTTGTTACAACACATGCTCTTTTG	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11564_Cjap_Sim2018_COI	DRWA-129	1	BE11564_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11564_Esin_And2018_Cyb	DRWA-129	1	BE11564_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-129	Cytb	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	ACCCCTCCTCATATCCAACCA	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11564_Mgig_Bot2012_COI	DRWA-129	1	BE11564_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	TCTTATTCGTTGGAGACTTTATAACCCT	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11564_Mjap_Gib2022_COI	DRWA-129	1	BE11564_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	AATTTAATTGTGACTAGGCATGGTCTG	130.90933
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11565_Cmae_Bot2011_COI	DRWA-130	1	BE11565_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	ATGAACAGTCTATCCTCCTTTAG	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11565_Hsan_Wil2021_COI	DRWA-130	1	BE11565_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	CCTGGGCCGGTATAGTAGGT	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11565_Mros_Gib2022_COI	DRWA-130	1	BE11565_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11565_Msal_Bot2012_COI	DRWA-130	1	BE11565_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	GYTAGTTCCRATGATGTTAGCTG	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11565_Mstr_Wil2021_COI	DRWA-130	1	BE11565_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	GTAGTGGTAACTACTCATGCTTTA	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11565_Pvir_Dia2013_COI	DRWA-130	1	BE11565_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	CTTAGTGGCATTAATTCGDAATCC	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11565_Rhar_Sim2018_COI	DRWA-130	1	BE11565_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	CTGCTGCTATTGCTCATG	130.91053
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11567_Aamu_Bax2006_COI	DRWA-132	1	BE11567_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	GCACAACCGGGATCTTTACTTC	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11567_Pcan_Bot2011_IGS	DRWA-132	1	BE11567_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-132	IGS	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	CGTAATCCTCAGTACTGGCTA	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11567_Pper_Dia2013_COI	DRWA-132	1	BE11567_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	CTTAGTGGCATTAATTCGDAATCC	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11567_Sspa_Oph2007_COI	DRWA-132	1	BE11567_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	GAAGGCCTTCCGATGATC	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11567_Upin_Bot2011_COI	DRWA-132	1	BE11567_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	CTTTAATTACAGCGTTTTTATTGTTGT	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11567_Vgib_Wil2025_COI	DRWA-132	1	BE11567_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	CCCCTCTTTACAAGTTCTTTAGGGT	130.91302
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11568_Asen_Wil2025_COI	DRWA-133	1	BE11568_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	GTTGTTGTTACAACACATGCTCTTTTG	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11568_Cjap_Sim2018_COI	DRWA-133	1	BE11568_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11568_Esin_And2018_Cyb	DRWA-133	1	BE11568_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-133	Cytb	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	ACCCCTCCTCATATCCAACCA	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11568_Mgig_Bot2012_COI	DRWA-133	1	BE11568_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	TCTTATTCGTTGGAGACTTTATAACCCT	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11568_Mjap_Gib2022_COI	DRWA-133	1	BE11568_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	AATTTAATTGTGACTAGGCATGGTCTG	130.91413
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11569_Cmae_Bot2011_COI	DRWA-134	1	BE11569_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	ATGAACAGTCTATCCTCCTTTAG	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11569_Hsan_Wil2021_COI	DRWA-134	1	BE11569_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	CCTGGGCCGGTATAGTAGGT	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11569_Mros_Gib2022_COI	DRWA-134	1	BE11569_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11569_Msal_Bot2012_COI	DRWA-134	1	BE11569_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	GYTAGTTCCRATGATGTTAGCTG	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11569_Mstr_Wil2021_COI	DRWA-134	1	BE11569_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	GTAGTGGTAACTACTCATGCTTTA	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11569_Pvir_Dia2013_COI	DRWA-134	1	BE11569_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	CTTAGTGGCATTAATTCGDAATCC	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11569_Rhar_Sim2018_COI	DRWA-134	1	BE11569_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	CTGCTGCTATTGCTCATG	130.91543
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11570_Aamu_Bax2006_COI	DRWA-135	1	BE11570_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	GCACAACCGGGATCTTTACTTC	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11570_Pcan_Bot2011_IGS	DRWA-135	1	BE11570_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-135	IGS	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	CGTAATCCTCAGTACTGGCTA	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11570_Pper_Dia2013_COI	DRWA-135	1	BE11570_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	CTTAGTGGCATTAATTCGDAATCC	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11570_Sspa_Oph2007_COI	DRWA-135	1	BE11570_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	GAAGGCCTTCCGATGATC	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11570_Upin_Bot2011_COI	DRWA-135	1	BE11570_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	CTTTAATTACAGCGTTTTTATTGTTGT	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11570_Vgib_Wil2025_COI	DRWA-135	1	BE11570_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	CCCCTCTTTACAAGTTCTTTAGGGT	130.90891
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11571_Asen_Wil2025_COI	DRWA-136	1	BE11571_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	GTTGTTGTTACAACACATGCTCTTTTG	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11571_Cjap_Sim2018_COI	DRWA-136	1	BE11571_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11571_Esin_And2018_Cyb	DRWA-136	1	BE11571_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-136	Cytb	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	ACCCCTCCTCATATCCAACCA	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11571_Mgig_Bot2012_COI	DRWA-136	1	BE11571_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	TCTTATTCGTTGGAGACTTTATAACCCT	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11571_Mjap_Gib2022_COI	DRWA-136	1	BE11571_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	AATTTAATTGTGACTAGGCATGGTCTG	130.91023
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11572_Cmae_Bot2011_COI	DRWA-137	1	BE11572_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	ATGAACAGTCTATCCTCCTTTAG	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11572_Hsan_Wil2021_COI	DRWA-137	1	BE11572_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	CCTGGGCCGGTATAGTAGGT	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11572_Mros_Gib2022_COI	DRWA-137	1	BE11572_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11572_Msal_Bot2012_COI	DRWA-137	1	BE11572_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	GYTAGTTCCRATGATGTTAGCTG	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11572_Mstr_Wil2021_COI	DRWA-137	1	BE11572_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	GTAGTGGTAACTACTCATGCTTTA	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11572_Pvir_Dia2013_COI	DRWA-137	1	BE11572_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	CTTAGTGGCATTAATTCGDAATCC	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11572_Rhar_Sim2018_COI	DRWA-137	1	BE11572_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	CTGCTGCTATTGCTCATG	130.91144
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11574_Aamu_Bax2006_COI	DRWA-139	1	BE11574_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	GCACAACCGGGATCTTTACTTC	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11574_Pcan_Bot2011_IGS	DRWA-139	1	BE11574_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-139	IGS	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	CGTAATCCTCAGTACTGGCTA	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11574_Pper_Dia2013_COI	DRWA-139	1	BE11574_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	CTTAGTGGCATTAATTCGDAATCC	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11574_Sspa_Oph2007_COI	DRWA-139	1	BE11574_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	GAAGGCCTTCCGATGATC	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11574_Upin_Bot2011_COI	DRWA-139	1	BE11574_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	CTTTAATTACAGCGTTTTTATTGTTGT	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11574_Vgib_Wil2025_COI	DRWA-139	1	BE11574_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	CCCCTCTTTACAAGTTCTTTAGGGT	130.91373
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11575_Asen_Wil2025_COI	DRWA-140	1	BE11575_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	GTTGTTGTTACAACACATGCTCTTTTG	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11575_Cjap_Sim2018_COI	DRWA-140	1	BE11575_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11575_Esin_And2018_Cyb	DRWA-140	1	BE11575_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-140	Cytb	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	ACCCCTCCTCATATCCAACCA	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11575_Mgig_Bot2012_COI	DRWA-140	1	BE11575_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	TCTTATTCGTTGGAGACTTTATAACCCT	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11575_Mjap_Gib2022_COI	DRWA-140	1	BE11575_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	AATTTAATTGTGACTAGGCATGGTCTG	130.91305
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11581_Aamu_Bax2006_COI	DRWA-037	1	BE11581_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	GCACAACCGGGATCTTTACTTC	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11581_Pcan_Bot2011_IGS	DRWA-037	1	BE11581_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-037	IGS	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	CGTAATCCTCAGTACTGGCTA	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11581_Pper_Dia2013_COI	DRWA-037	1	BE11581_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	CTTAGTGGCATTAATTCGDAATCC	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11581_Sspa_Oph2007_COI	DRWA-037	1	BE11581_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	GAAGGCCTTCCGATGATC	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11581_Upin_Bot2011_COI	DRWA-037	1	BE11581_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	CTTTAATTACAGCGTTTTTATTGTTGT	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11581_Vgib_Wil2025_COI	DRWA-037	1	BE11581_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	CCCCTCTTTACAAGTTCTTTAGGGT	130.92944
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11582_Asen_Wil2025_COI	DRWA-038	1	BE11582_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	GTTGTTGTTACAACACATGCTCTTTTG	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11582_Cjap_Sim2018_COI	DRWA-038	1	BE11582_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11582_Esin_And2018_Cyb	DRWA-038	1	BE11582_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-038	Cytb	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	ACCCCTCCTCATATCCAACCA	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11582_Mgig_Bot2012_COI	DRWA-038	1	BE11582_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	TCTTATTCGTTGGAGACTTTATAACCCT	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11582_Mjap_Gib2022_COI	DRWA-038	1	BE11582_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	AATTTAATTGTGACTAGGCATGGTCTG	130.93065
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11583_Cmae_Bot2011_COI	DRWA-039	1	BE11583_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	ATGAACAGTCTATCCTCCTTTAG	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11583_Hsan_Wil2021_COI	DRWA-039	1	BE11583_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	CCTGGGCCGGTATAGTAGGT	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11583_Mros_Gib2022_COI	DRWA-039	1	BE11583_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11583_Msal_Bot2012_COI	DRWA-039	1	BE11583_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	GYTAGTTCCRATGATGTTAGCTG	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11583_Mstr_Wil2021_COI	DRWA-039	1	BE11583_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	GTAGTGGTAACTACTCATGCTTTA	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11583_Pvir_Dia2013_COI	DRWA-039	1	BE11583_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	CTTAGTGGCATTAATTCGDAATCC	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11583_Rhar_Sim2018_COI	DRWA-039	1	BE11583_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	CTGCTGCTATTGCTCATG	130.93139
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11585_Aamu_Bax2006_COI	DRWA-041	1	BE11585_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	GCACAACCGGGATCTTTACTTC	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11585_Pcan_Bot2011_IGS	DRWA-041	1	BE11585_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-041	IGS	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	CGTAATCCTCAGTACTGGCTA	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11585_Pper_Dia2013_COI	DRWA-041	1	BE11585_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	CTTAGTGGCATTAATTCGDAATCC	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11585_Sspa_Oph2007_COI	DRWA-041	1	BE11585_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	GAAGGCCTTCCGATGATC	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11585_Upin_Bot2011_COI	DRWA-041	1	BE11585_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	CTTTAATTACAGCGTTTTTATTGTTGT	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11585_Vgib_Wil2025_COI	DRWA-041	1	BE11585_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	CCCCTCTTTACAAGTTCTTTAGGGT	130.93095
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11586_Asen_Wil2025_COI	DRWA-042	1	BE11586_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	GTTGTTGTTACAACACATGCTCTTTTG	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11586_Cjap_Sim2018_COI	DRWA-042	1	BE11586_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11586_Esin_And2018_Cyb	DRWA-042	1	BE11586_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-042	Cytb	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	ACCCCTCCTCATATCCAACCA	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11586_Mgig_Bot2012_COI	DRWA-042	1	BE11586_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	TCTTATTCGTTGGAGACTTTATAACCCT	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11586_Mjap_Gib2022_COI	DRWA-042	1	BE11586_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	AATTTAATTGTGACTAGGCATGGTCTG	130.93261
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11587_Cmae_Bot2011_COI	DRWA-043	1	BE11587_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	ATGAACAGTCTATCCTCCTTTAG	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11587_Hsan_Wil2021_COI	DRWA-043	1	BE11587_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	CCTGGGCCGGTATAGTAGGT	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11587_Mros_Gib2022_COI	DRWA-043	1	BE11587_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11587_Msal_Bot2012_COI	DRWA-043	1	BE11587_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	GYTAGTTCCRATGATGTTAGCTG	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11587_Mstr_Wil2021_COI	DRWA-043	1	BE11587_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	GTAGTGGTAACTACTCATGCTTTA	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11587_Pvir_Dia2013_COI	DRWA-043	1	BE11587_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	CTTAGTGGCATTAATTCGDAATCC	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11587_Rhar_Sim2018_COI	DRWA-043	1	BE11587_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	CTGCTGCTATTGCTCATG	130.9301
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11589_Aamu_Bax2006_COI	DRWA-045	1	BE11589_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	GCACAACCGGGATCTTTACTTC	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11589_Pcan_Bot2011_IGS	DRWA-045	1	BE11589_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-045	IGS	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	CGTAATCCTCAGTACTGGCTA	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11589_Pper_Dia2013_COI	DRWA-045	1	BE11589_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	CTTAGTGGCATTAATTCGDAATCC	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11589_Sspa_Oph2007_COI	DRWA-045	1	BE11589_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	GAAGGCCTTCCGATGATC	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11589_Upin_Bot2011_COI	DRWA-045	1	BE11589_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	CTTTAATTACAGCGTTTTTATTGTTGT	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11589_Vgib_Wil2025_COI	DRWA-045	1	BE11589_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	CCCCTCTTTACAAGTTCTTTAGGGT	130.9322
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11590_Cmae_Bot2011_COI	DRWA-046	1	BE11590_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	ATGAACAGTCTATCCTCCTTTAG	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11590_Hsan_Wil2021_COI	DRWA-046	1	BE11590_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	CCTGGGCCGGTATAGTAGGT	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11590_Mros_Gib2022_COI	DRWA-046	1	BE11590_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11590_Msal_Bot2012_COI	DRWA-046	1	BE11590_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	GYTAGTTCCRATGATGTTAGCTG	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11590_Mstr_Wil2021_COI	DRWA-046	1	BE11590_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	GTAGTGGTAACTACTCATGCTTTA	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11590_Pvir_Dia2013_COI	DRWA-046	1	BE11590_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	CTTAGTGGCATTAATTCGDAATCC	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11590_Rhar_Sim2018_COI	DRWA-046	1	BE11590_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	CTGCTGCTATTGCTCATG	130.92965
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11592_Aamu_Bax2006_COI	DRWA-048	1	BE11592_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	GCACAACCGGGATCTTTACTTC	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11592_Pcan_Bot2011_IGS	DRWA-048	1	BE11592_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-048	IGS	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	CGTAATCCTCAGTACTGGCTA	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11592_Pper_Dia2013_COI	DRWA-048	1	BE11592_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	CTTAGTGGCATTAATTCGDAATCC	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11592_Sspa_Oph2007_COI	DRWA-048	1	BE11592_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	GAAGGCCTTCCGATGATC	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11592_Upin_Bot2011_COI	DRWA-048	1	BE11592_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	CTTTAATTACAGCGTTTTTATTGTTGT	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11592_Vgib_Wil2025_COI	DRWA-048	1	BE11592_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	CCCCTCTTTACAAGTTCTTTAGGGT	130.9317
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11593_Asen_Wil2025_COI	DRWA-049	1	BE11593_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	GTTGTTGTTACAACACATGCTCTTTTG	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11593_Cjap_Sim2018_COI	DRWA-049	1	BE11593_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11593_Esin_And2018_Cyb	DRWA-049	1	BE11593_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-049	Cytb	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	ACCCCTCCTCATATCCAACCA	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11593_Mgig_Bot2012_COI	DRWA-049	1	BE11593_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	TCTTATTCGTTGGAGACTTTATAACCCT	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11593_Mjap_Gib2022_COI	DRWA-049	1	BE11593_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	AATTTAATTGTGACTAGGCATGGTCTG	130.92916
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11594_Cmae_Bot2011_COI	DRWA-050	1	BE11594_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	ATGAACAGTCTATCCTCCTTTAG	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11594_Hsan_Wil2021_COI	DRWA-050	1	BE11594_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	CCTGGGCCGGTATAGTAGGT	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11594_Mros_Gib2022_COI	DRWA-050	1	BE11594_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11594_Msal_Bot2012_COI	DRWA-050	1	BE11594_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	GYTAGTTCCRATGATGTTAGCTG	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11594_Mstr_Wil2021_COI	DRWA-050	1	BE11594_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	GTAGTGGTAACTACTCATGCTTTA	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11594_Pvir_Dia2013_COI	DRWA-050	1	BE11594_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	CTTAGTGGCATTAATTCGDAATCC	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11594_Rhar_Sim2018_COI	DRWA-050	1	BE11594_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	CTGCTGCTATTGCTCATG	130.93018
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11596_Aamu_Bax2006_COI	DRWA-052	1	BE11596_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	GCACAACCGGGATCTTTACTTC	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11596_Pcan_Bot2011_IGS	DRWA-052	1	BE11596_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-052	IGS	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	CGTAATCCTCAGTACTGGCTA	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11596_Pper_Dia2013_COI	DRWA-052	1	BE11596_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	CTTAGTGGCATTAATTCGDAATCC	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11596_Sspa_Oph2007_COI	DRWA-052	1	BE11596_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	GAAGGCCTTCCGATGATC	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11596_Upin_Bot2011_COI	DRWA-052	1	BE11596_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	CTTTAATTACAGCGTTTTTATTGTTGT	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11596_Vgib_Wil2025_COI	DRWA-052	1	BE11596_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	CCCCTCTTTACAAGTTCTTTAGGGT	130.9322
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11597_Asen_Wil2025_COI	DRWA-053	1	BE11597_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	GTTGTTGTTACAACACATGCTCTTTTG	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11597_Cjap_Sim2018_COI	DRWA-053	1	BE11597_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11597_Esin_And2018_Cyb	DRWA-053	1	BE11597_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-053	Cytb	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	ACCCCTCCTCATATCCAACCA	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11597_Mgig_Bot2012_COI	DRWA-053	1	BE11597_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	TCTTATTCGTTGGAGACTTTATAACCCT	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11597_Mjap_Gib2022_COI	DRWA-053	1	BE11597_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	AATTTAATTGTGACTAGGCATGGTCTG	130.9286
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11598_Cmae_Bot2011_COI	DRWA-054	1	BE11598_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	ATGAACAGTCTATCCTCCTTTAG	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11598_Hsan_Wil2021_COI	DRWA-054	1	BE11598_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	CCTGGGCCGGTATAGTAGGT	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11598_Mros_Gib2022_COI	DRWA-054	1	BE11598_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11598_Msal_Bot2012_COI	DRWA-054	1	BE11598_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	GYTAGTTCCRATGATGTTAGCTG	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11598_Mstr_Wil2021_COI	DRWA-054	1	BE11598_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	GTAGTGGTAACTACTCATGCTTTA	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11598_Pvir_Dia2013_COI	DRWA-054	1	BE11598_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	CTTAGTGGCATTAATTCGDAATCC	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11598_Rhar_Sim2018_COI	DRWA-054	1	BE11598_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	CTGCTGCTATTGCTCATG	130.92966
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11600_Aamu_Bax2006_COI	DRWA-056	1	BE11600_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	GCACAACCGGGATCTTTACTTC	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11600_Pcan_Bot2011_IGS	DRWA-056	1	BE11600_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-056	IGS	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	CGTAATCCTCAGTACTGGCTA	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11600_Pper_Dia2013_COI	DRWA-056	1	BE11600_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	CTTAGTGGCATTAATTCGDAATCC	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11600_Sspa_Oph2007_COI	DRWA-056	1	BE11600_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	GAAGGCCTTCCGATGATC	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11600_Upin_Bot2011_COI	DRWA-056	1	BE11600_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	CTTTAATTACAGCGTTTTTATTGTTGT	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11600_Vgib_Wil2025_COI	DRWA-056	1	BE11600_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	CCCCTCTTTACAAGTTCTTTAGGGT	130.93181
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11601_Asen_Wil2025_COI	DRWA-057	1	BE11601_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	GTTGTTGTTACAACACATGCTCTTTTG	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11601_Cjap_Sim2018_COI	DRWA-057	1	BE11601_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11601_Esin_And2018_Cyb	DRWA-057	1	BE11601_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-057	Cytb	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	ACCCCTCCTCATATCCAACCA	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11601_Mgig_Bot2012_COI	DRWA-057	1	BE11601_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	TCTTATTCGTTGGAGACTTTATAACCCT	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11601_Mjap_Gib2022_COI	DRWA-057	1	BE11601_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	AATTTAATTGTGACTAGGCATGGTCTG	130.92821
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11602_Cmae_Bot2011_COI	DRWA-058	1	BE11602_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	ATGAACAGTCTATCCTCCTTTAG	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11602_Hsan_Wil2021_COI	DRWA-058	1	BE11602_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	CCTGGGCCGGTATAGTAGGT	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11602_Mros_Gib2022_COI	DRWA-058	1	BE11602_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11602_Msal_Bot2012_COI	DRWA-058	1	BE11602_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	GYTAGTTCCRATGATGTTAGCTG	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11602_Mstr_Wil2021_COI	DRWA-058	1	BE11602_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	GTAGTGGTAACTACTCATGCTTTA	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11602_Pvir_Dia2013_COI	DRWA-058	1	BE11602_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	CTTAGTGGCATTAATTCGDAATCC	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11602_Rhar_Sim2018_COI	DRWA-058	1	BE11602_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	CTGCTGCTATTGCTCATG	130.92919
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11604_Aamu_Bax2006_COI	DRWA-060	1	BE11604_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	GCACAACCGGGATCTTTACTTC	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11604_Pcan_Bot2011_IGS	DRWA-060	1	BE11604_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-060	IGS	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	CGTAATCCTCAGTACTGGCTA	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11604_Pper_Dia2013_COI	DRWA-060	1	BE11604_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	CTTAGTGGCATTAATTCGDAATCC	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11604_Sspa_Oph2007_COI	DRWA-060	1	BE11604_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	GAAGGCCTTCCGATGATC	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11604_Upin_Bot2011_COI	DRWA-060	1	BE11604_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	CTTTAATTACAGCGTTTTTATTGTTGT	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11604_Vgib_Wil2025_COI	DRWA-060	1	BE11604_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	CCCCTCTTTACAAGTTCTTTAGGGT	130.93128
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11605_Asen_Wil2025_COI	DRWA-061	1	BE11605_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	GTTGTTGTTACAACACATGCTCTTTTG	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11605_Cjap_Sim2018_COI	DRWA-061	1	BE11605_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11605_Esin_And2018_Cyb	DRWA-061	1	BE11605_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-061	Cytb	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	ACCCCTCCTCATATCCAACCA	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11605_Mgig_Bot2012_COI	DRWA-061	1	BE11605_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	TCTTATTCGTTGGAGACTTTATAACCCT	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11605_Mjap_Gib2022_COI	DRWA-061	1	BE11605_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	AATTTAATTGTGACTAGGCATGGTCTG	130.92762
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11606_Cmae_Bot2011_COI	DRWA-062	1	BE11606_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	ATGAACAGTCTATCCTCCTTTAG	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11606_Hsan_Wil2021_COI	DRWA-062	1	BE11606_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	CCTGGGCCGGTATAGTAGGT	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11606_Mros_Gib2022_COI	DRWA-062	1	BE11606_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11606_Msal_Bot2012_COI	DRWA-062	1	BE11606_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	GYTAGTTCCRATGATGTTAGCTG	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11606_Mstr_Wil2021_COI	DRWA-062	1	BE11606_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	GTAGTGGTAACTACTCATGCTTTA	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11606_Pvir_Dia2013_COI	DRWA-062	1	BE11606_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	CTTAGTGGCATTAATTCGDAATCC	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11606_Rhar_Sim2018_COI	DRWA-062	1	BE11606_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	CTGCTGCTATTGCTCATG	130.92868
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11608_Aamu_Bax2006_COI	DRWA-064	1	BE11608_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	GCACAACCGGGATCTTTACTTC	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11608_Pcan_Bot2011_IGS	DRWA-064	1	BE11608_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-064	IGS	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	CGTAATCCTCAGTACTGGCTA	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11608_Pper_Dia2013_COI	DRWA-064	1	BE11608_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	CTTAGTGGCATTAATTCGDAATCC	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11608_Sspa_Oph2007_COI	DRWA-064	1	BE11608_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	GAAGGCCTTCCGATGATC	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11608_Upin_Bot2011_COI	DRWA-064	1	BE11608_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	CTTTAATTACAGCGTTTTTATTGTTGT	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11608_Vgib_Wil2025_COI	DRWA-064	1	BE11608_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	CCCCTCTTTACAAGTTCTTTAGGGT	130.93076
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11609_Asen_Wil2025_COI	DRWA-065	1	BE11609_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	GTTGTTGTTACAACACATGCTCTTTTG	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11609_Cjap_Sim2018_COI	DRWA-065	1	BE11609_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11609_Esin_And2018_Cyb	DRWA-065	1	BE11609_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-065	Cytb	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	ACCCCTCCTCATATCCAACCA	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11609_Mgig_Bot2012_COI	DRWA-065	1	BE11609_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	TCTTATTCGTTGGAGACTTTATAACCCT	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11609_Mjap_Gib2022_COI	DRWA-065	1	BE11609_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	AATTTAATTGTGACTAGGCATGGTCTG	130.92723
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11615_Aamu_Bax2006_COI	DRWA-067	1	BE11615_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	GCACAACCGGGATCTTTACTTC	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11615_Pcan_Bot2011_IGS	DRWA-067	1	BE11615_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-067	IGS	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	CGTAATCCTCAGTACTGGCTA	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11615_Pper_Dia2013_COI	DRWA-067	1	BE11615_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	CTTAGTGGCATTAATTCGDAATCC	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11615_Sspa_Oph2007_COI	DRWA-067	1	BE11615_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	GAAGGCCTTCCGATGATC	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11615_Upin_Bot2011_COI	DRWA-067	1	BE11615_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	CTTTAATTACAGCGTTTTTATTGTTGT	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11615_Vgib_Wil2025_COI	DRWA-067	1	BE11615_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	CCCCTCTTTACAAGTTCTTTAGGGT	130.92913
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11616_Asen_Wil2025_COI	DRWA-068	1	BE11616_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	GTTGTTGTTACAACACATGCTCTTTTG	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11616_Cjap_Sim2018_COI	DRWA-068	1	BE11616_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11616_Esin_And2018_Cyb	DRWA-068	1	BE11616_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-068	Cytb	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	ACCCCTCCTCATATCCAACCA	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11616_Mgig_Bot2012_COI	DRWA-068	1	BE11616_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	TCTTATTCGTTGGAGACTTTATAACCCT	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11616_Mjap_Gib2022_COI	DRWA-068	1	BE11616_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	AATTTAATTGTGACTAGGCATGGTCTG	130.93016
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11617_Cmae_Bot2011_COI	DRWA-069	1	BE11617_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	ATGAACAGTCTATCCTCCTTTAG	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11617_Hsan_Wil2021_COI	DRWA-069	1	BE11617_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	CCTGGGCCGGTATAGTAGGT	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11617_Mros_Gib2022_COI	DRWA-069	1	BE11617_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11617_Msal_Bot2012_COI	DRWA-069	1	BE11617_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	GYTAGTTCCRATGATGTTAGCTG	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11617_Mstr_Wil2021_COI	DRWA-069	1	BE11617_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	GTAGTGGTAACTACTCATGCTTTA	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11617_Pvir_Dia2013_COI	DRWA-069	1	BE11617_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	CTTAGTGGCATTAATTCGDAATCC	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11617_Rhar_Sim2018_COI	DRWA-069	1	BE11617_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	CTGCTGCTATTGCTCATG	130.92765
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11619_Aamu_Bax2006_COI	DRWA-071	1	BE11619_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	GCACAACCGGGATCTTTACTTC	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11619_Pcan_Bot2011_IGS	DRWA-071	1	BE11619_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-071	IGS	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	CGTAATCCTCAGTACTGGCTA	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11619_Pper_Dia2013_COI	DRWA-071	1	BE11619_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	CTTAGTGGCATTAATTCGDAATCC	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11619_Sspa_Oph2007_COI	DRWA-071	1	BE11619_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	GAAGGCCTTCCGATGATC	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11619_Upin_Bot2011_COI	DRWA-071	1	BE11619_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	CTTTAATTACAGCGTTTTTATTGTTGT	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11619_Vgib_Wil2025_COI	DRWA-071	1	BE11619_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	CCCCTCTTTACAAGTTCTTTAGGGT	130.88164
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11620_Cmae_Bot2011_COI	DRWA-072	1	BE11620_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	ATGAACAGTCTATCCTCCTTTAG	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11620_Hsan_Wil2021_COI	DRWA-072	1	BE11620_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	CCTGGGCCGGTATAGTAGGT	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11620_Mros_Gib2022_COI	DRWA-072	1	BE11620_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11620_Msal_Bot2012_COI	DRWA-072	1	BE11620_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	GYTAGTTCCRATGATGTTAGCTG	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11620_Mstr_Wil2021_COI	DRWA-072	1	BE11620_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	GTAGTGGTAACTACTCATGCTTTA	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11620_Pvir_Dia2013_COI	DRWA-072	1	BE11620_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	CTTAGTGGCATTAATTCGDAATCC	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11620_Rhar_Sim2018_COI	DRWA-072	1	BE11620_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	CTGCTGCTATTGCTCATG	130.8886
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11622_Aamu_Bax2006_COI	DRWA-074	1	BE11622_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	GCACAACCGGGATCTTTACTTC	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11622_Pcan_Bot2011_IGS	DRWA-074	1	BE11622_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-074	IGS	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	CGTAATCCTCAGTACTGGCTA	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11622_Pper_Dia2013_COI	DRWA-074	1	BE11622_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	CTTAGTGGCATTAATTCGDAATCC	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11622_Sspa_Oph2007_COI	DRWA-074	1	BE11622_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	GAAGGCCTTCCGATGATC	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11622_Upin_Bot2011_COI	DRWA-074	1	BE11622_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	CTTTAATTACAGCGTTTTTATTGTTGT	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11622_Vgib_Wil2025_COI	DRWA-074	1	BE11622_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	CCCCTCTTTACAAGTTCTTTAGGGT	130.89165
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11623_Asen_Wil2025_COI	DRWA-075	1	BE11623_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	GTTGTTGTTACAACACATGCTCTTTTG	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11623_Cjap_Sim2018_COI	DRWA-075	1	BE11623_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11623_Esin_And2018_Cyb	DRWA-075	1	BE11623_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-075	Cytb	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	ACCCCTCCTCATATCCAACCA	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11623_Mgig_Bot2012_COI	DRWA-075	1	BE11623_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	TCTTATTCGTTGGAGACTTTATAACCCT	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11623_Mjap_Gib2022_COI	DRWA-075	1	BE11623_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	AATTTAATTGTGACTAGGCATGGTCTG	130.87952
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11624_Cmae_Bot2011_COI	DRWA-076	1	BE11624_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	ATGAACAGTCTATCCTCCTTTAG	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11624_Hsan_Wil2021_COI	DRWA-076	1	BE11624_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	CCTGGGCCGGTATAGTAGGT	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11624_Mros_Gib2022_COI	DRWA-076	1	BE11624_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11624_Msal_Bot2012_COI	DRWA-076	1	BE11624_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	GYTAGTTCCRATGATGTTAGCTG	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11624_Mstr_Wil2021_COI	DRWA-076	1	BE11624_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	GTAGTGGTAACTACTCATGCTTTA	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11624_Pvir_Dia2013_COI	DRWA-076	1	BE11624_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	CTTAGTGGCATTAATTCGDAATCC	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11624_Rhar_Sim2018_COI	DRWA-076	1	BE11624_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	CTGCTGCTATTGCTCATG	130.88109
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11626_Aamu_Bax2006_COI	DRWA-078	1	BE11626_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	GCACAACCGGGATCTTTACTTC	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11626_Pcan_Bot2011_IGS	DRWA-078	1	BE11626_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-078	IGS	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	CGTAATCCTCAGTACTGGCTA	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11626_Pper_Dia2013_COI	DRWA-078	1	BE11626_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	CTTAGTGGCATTAATTCGDAATCC	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11626_Sspa_Oph2007_COI	DRWA-078	1	BE11626_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	GAAGGCCTTCCGATGATC	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11626_Upin_Bot2011_COI	DRWA-078	1	BE11626_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	CTTTAATTACAGCGTTTTTATTGTTGT	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11626_Vgib_Wil2025_COI	DRWA-078	1	BE11626_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	CCCCTCTTTACAAGTTCTTTAGGGT	130.88409
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11627_Asen_Wil2025_COI	DRWA-079	1	BE11627_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	GTTGTTGTTACAACACATGCTCTTTTG	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11627_Cjap_Sim2018_COI	DRWA-079	1	BE11627_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11627_Esin_And2018_Cyb	DRWA-079	1	BE11627_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-079	Cytb	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	ACCCCTCCTCATATCCAACCA	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11627_Mgig_Bot2012_COI	DRWA-079	1	BE11627_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	TCTTATTCGTTGGAGACTTTATAACCCT	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11627_Mjap_Gib2022_COI	DRWA-079	1	BE11627_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	AATTTAATTGTGACTAGGCATGGTCTG	130.88853
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11628_Cmae_Bot2011_COI	DRWA-080	1	BE11628_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	ATGAACAGTCTATCCTCCTTTAG	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11628_Hsan_Wil2021_COI	DRWA-080	1	BE11628_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	CCTGGGCCGGTATAGTAGGT	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11628_Mros_Gib2022_COI	DRWA-080	1	BE11628_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11628_Msal_Bot2012_COI	DRWA-080	1	BE11628_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	GYTAGTTCCRATGATGTTAGCTG	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11628_Mstr_Wil2021_COI	DRWA-080	1	BE11628_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	GTAGTGGTAACTACTCATGCTTTA	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11628_Pvir_Dia2013_COI	DRWA-080	1	BE11628_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	CTTAGTGGCATTAATTCGDAATCC	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11628_Rhar_Sim2018_COI	DRWA-080	1	BE11628_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	CTGCTGCTATTGCTCATG	130.88966
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11630_Asen_Wil2025_COI	DRWA-082	1	BE11630_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	GTTGTTGTTACAACACATGCTCTTTTG	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11630_Cjap_Sim2018_COI	DRWA-082	1	BE11630_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11630_Esin_And2018_Cyb	DRWA-082	1	BE11630_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-082	Cytb	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	ACCCCTCCTCATATCCAACCA	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11630_Mgig_Bot2012_COI	DRWA-082	1	BE11630_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	TCTTATTCGTTGGAGACTTTATAACCCT	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11630_Mjap_Gib2022_COI	DRWA-082	1	BE11630_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	AATTTAATTGTGACTAGGCATGGTCTG	130.88028
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11631_Cmae_Bot2011_COI	DRWA-083	1	BE11631_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	ATGAACAGTCTATCCTCCTTTAG	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11631_Hsan_Wil2021_COI	DRWA-083	1	BE11631_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	CCTGGGCCGGTATAGTAGGT	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11631_Mros_Gib2022_COI	DRWA-083	1	BE11631_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11631_Msal_Bot2012_COI	DRWA-083	1	BE11631_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	GYTAGTTCCRATGATGTTAGCTG	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11631_Mstr_Wil2021_COI	DRWA-083	1	BE11631_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	GTAGTGGTAACTACTCATGCTTTA	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11631_Pvir_Dia2013_COI	DRWA-083	1	BE11631_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	CTTAGTGGCATTAATTCGDAATCC	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11631_Rhar_Sim2018_COI	DRWA-083	1	BE11631_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	CTGCTGCTATTGCTCATG	130.88173
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11633_Aamu_Bax2006_COI	DRWA-085	1	BE11633_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	GCACAACCGGGATCTTTACTTC	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11633_Pcan_Bot2011_IGS	DRWA-085	1	BE11633_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-085	IGS	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	CGTAATCCTCAGTACTGGCTA	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11633_Pper_Dia2013_COI	DRWA-085	1	BE11633_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	CTTAGTGGCATTAATTCGDAATCC	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11633_Sspa_Oph2007_COI	DRWA-085	1	BE11633_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	GAAGGCCTTCCGATGATC	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11633_Upin_Bot2011_COI	DRWA-085	1	BE11633_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	CTTTAATTACAGCGTTTTTATTGTTGT	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11633_Vgib_Wil2025_COI	DRWA-085	1	BE11633_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	CCCCTCTTTACAAGTTCTTTAGGGT	130.87809
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11634_Asen_Wil2025_COI	DRWA-086	1	BE11634_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	GTTGTTGTTACAACACATGCTCTTTTG	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11634_Cjap_Sim2018_COI	DRWA-086	1	BE11634_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11634_Esin_And2018_Cyb	DRWA-086	1	BE11634_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-086	Cytb	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	ACCCCTCCTCATATCCAACCA	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11634_Mgig_Bot2012_COI	DRWA-086	1	BE11634_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	TCTTATTCGTTGGAGACTTTATAACCCT	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11634_Mjap_Gib2022_COI	DRWA-086	1	BE11634_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	AATTTAATTGTGACTAGGCATGGTCTG	130.87958
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11635_Cmae_Bot2011_COI	DRWA-087	1	BE11635_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	ATGAACAGTCTATCCTCCTTTAG	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11635_Hsan_Wil2021_COI	DRWA-087	1	BE11635_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	CCTGGGCCGGTATAGTAGGT	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11635_Mros_Gib2022_COI	DRWA-087	1	BE11635_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11635_Msal_Bot2012_COI	DRWA-087	1	BE11635_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	GYTAGTTCCRATGATGTTAGCTG	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11635_Mstr_Wil2021_COI	DRWA-087	1	BE11635_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	GTAGTGGTAACTACTCATGCTTTA	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11635_Pvir_Dia2013_COI	DRWA-087	1	BE11635_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	CTTAGTGGCATTAATTCGDAATCC	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11635_Rhar_Sim2018_COI	DRWA-087	1	BE11635_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	CTGCTGCTATTGCTCATG	130.88106
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11637_Aamu_Bax2006_COI	DRWA-089	1	BE11637_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	GCACAACCGGGATCTTTACTTC	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11637_Pcan_Bot2011_IGS	DRWA-089	1	BE11637_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-089	IGS	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	CGTAATCCTCAGTACTGGCTA	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11637_Pper_Dia2013_COI	DRWA-089	1	BE11637_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	CTTAGTGGCATTAATTCGDAATCC	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11637_Sspa_Oph2007_COI	DRWA-089	1	BE11637_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	GAAGGCCTTCCGATGATC	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11637_Upin_Bot2011_COI	DRWA-089	1	BE11637_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	CTTTAATTACAGCGTTTTTATTGTTGT	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11637_Vgib_Wil2025_COI	DRWA-089	1	BE11637_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	CCCCTCTTTACAAGTTCTTTAGGGT	130.8761
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11638_Asen_Wil2025_COI	DRWA-090	1	BE11638_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	GTTGTTGTTACAACACATGCTCTTTTG	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11638_Cjap_Sim2018_COI	DRWA-090	1	BE11638_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11638_Esin_And2018_Cyb	DRWA-090	1	BE11638_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-090	Cytb	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	ACCCCTCCTCATATCCAACCA	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11638_Mgig_Bot2012_COI	DRWA-090	1	BE11638_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	TCTTATTCGTTGGAGACTTTATAACCCT	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11638_Mjap_Gib2022_COI	DRWA-090	1	BE11638_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	AATTTAATTGTGACTAGGCATGGTCTG	130.87752
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11639_Cmae_Bot2011_COI	DRWA-091	1	BE11639_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	ATGAACAGTCTATCCTCCTTTAG	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11639_Hsan_Wil2021_COI	DRWA-091	1	BE11639_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	CCTGGGCCGGTATAGTAGGT	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11639_Mros_Gib2022_COI	DRWA-091	1	BE11639_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11639_Msal_Bot2012_COI	DRWA-091	1	BE11639_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	GYTAGTTCCRATGATGTTAGCTG	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11639_Mstr_Wil2021_COI	DRWA-091	1	BE11639_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	GTAGTGGTAACTACTCATGCTTTA	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11639_Pvir_Dia2013_COI	DRWA-091	1	BE11639_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	CTTAGTGGCATTAATTCGDAATCC	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11639_Rhar_Sim2018_COI	DRWA-091	1	BE11639_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	CTGCTGCTATTGCTCATG	130.87904
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11640_Aamu_Bax2006_COI	DRWA-092	1	BE11640_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	GCACAACCGGGATCTTTACTTC	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11640_Pcan_Bot2011_IGS	DRWA-092	1	BE11640_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-092	IGS	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	CGTAATCCTCAGTACTGGCTA	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11640_Pper_Dia2013_COI	DRWA-092	1	BE11640_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	CTTAGTGGCATTAATTCGDAATCC	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11640_Sspa_Oph2007_COI	DRWA-092	1	BE11640_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	GAAGGCCTTCCGATGATC	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11640_Upin_Bot2011_COI	DRWA-092	1	BE11640_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	CTTTAATTACAGCGTTTTTATTGTTGT	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11640_Vgib_Wil2025_COI	DRWA-092	1	BE11640_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	CCCCTCTTTACAAGTTCTTTAGGGT	130.87405
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11641_Asen_Wil2025_COI	DRWA-093	1	BE11641_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	GTTGTTGTTACAACACATGCTCTTTTG	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11641_Cjap_Sim2018_COI	DRWA-093	1	BE11641_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11641_Esin_And2018_Cyb	DRWA-093	1	BE11641_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-093	Cytb	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	ACCCCTCCTCATATCCAACCA	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11641_Mgig_Bot2012_COI	DRWA-093	1	BE11641_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	TCTTATTCGTTGGAGACTTTATAACCCT	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11641_Mjap_Gib2022_COI	DRWA-093	1	BE11641_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	AATTTAATTGTGACTAGGCATGGTCTG	130.87532
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11642_Cmae_Bot2011_COI	DRWA-094	1	BE11642_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	ATGAACAGTCTATCCTCCTTTAG	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11642_Hsan_Wil2021_COI	DRWA-094	1	BE11642_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	CCTGGGCCGGTATAGTAGGT	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11642_Mros_Gib2022_COI	DRWA-094	1	BE11642_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11642_Msal_Bot2012_COI	DRWA-094	1	BE11642_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	GYTAGTTCCRATGATGTTAGCTG	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11642_Mstr_Wil2021_COI	DRWA-094	1	BE11642_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	GTAGTGGTAACTACTCATGCTTTA	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11642_Pvir_Dia2013_COI	DRWA-094	1	BE11642_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	CTTAGTGGCATTAATTCGDAATCC	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11642_Rhar_Sim2018_COI	DRWA-094	1	BE11642_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	CTGCTGCTATTGCTCATG	130.8769
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11644_Aamu_Bax2006_COI	DRWA-096	1	BE11644_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	GCACAACCGGGATCTTTACTTC	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11644_Pcan_Bot2011_IGS	DRWA-096	1	BE11644_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-096	IGS	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	CGTAATCCTCAGTACTGGCTA	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11644_Pper_Dia2013_COI	DRWA-096	1	BE11644_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	CTTAGTGGCATTAATTCGDAATCC	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11644_Sspa_Oph2007_COI	DRWA-096	1	BE11644_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	GAAGGCCTTCCGATGATC	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11644_Upin_Bot2011_COI	DRWA-096	1	BE11644_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	CTTTAATTACAGCGTTTTTATTGTTGT	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11644_Vgib_Wil2025_COI	DRWA-096	1	BE11644_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	CCCCTCTTTACAAGTTCTTTAGGGT	130.87454
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11645_Asen_Wil2025_COI	DRWA-097	1	BE11645_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	GTTGTTGTTACAACACATGCTCTTTTG	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11645_Cjap_Sim2018_COI	DRWA-097	1	BE11645_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11645_Esin_And2018_Cyb	DRWA-097	1	BE11645_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-097	Cytb	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	ACCCCTCCTCATATCCAACCA	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11645_Mgig_Bot2012_COI	DRWA-097	1	BE11645_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	TCTTATTCGTTGGAGACTTTATAACCCT	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11645_Mjap_Gib2022_COI	DRWA-097	1	BE11645_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	AATTTAATTGTGACTAGGCATGGTCTG	130.87592
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11646_Cmae_Bot2011_COI	DRWA-098	1	BE11646_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	ATGAACAGTCTATCCTCCTTTAG	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11646_Hsan_Wil2021_COI	DRWA-098	1	BE11646_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	CCTGGGCCGGTATAGTAGGT	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11646_Mros_Gib2022_COI	DRWA-098	1	BE11646_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11646_Msal_Bot2012_COI	DRWA-098	1	BE11646_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	GYTAGTTCCRATGATGTTAGCTG	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11646_Mstr_Wil2021_COI	DRWA-098	1	BE11646_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	GTAGTGGTAACTACTCATGCTTTA	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11646_Pvir_Dia2013_COI	DRWA-098	1	BE11646_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	CTTAGTGGCATTAATTCGDAATCC	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11646_Rhar_Sim2018_COI	DRWA-098	1	BE11646_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	CTGCTGCTATTGCTCATG	130.87388
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11648_Aamu_Bax2006_COI	DRWA-100	1	BE11648_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	GCACAACCGGGATCTTTACTTC	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11648_Pcan_Bot2011_IGS	DRWA-100	1	BE11648_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-100	IGS	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	CGTAATCCTCAGTACTGGCTA	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11648_Pper_Dia2013_COI	DRWA-100	1	BE11648_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	CTTAGTGGCATTAATTCGDAATCC	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11648_Sspa_Oph2007_COI	DRWA-100	1	BE11648_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	GAAGGCCTTCCGATGATC	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11648_Upin_Bot2011_COI	DRWA-100	1	BE11648_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	CTTTAATTACAGCGTTTTTATTGTTGT	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11648_Vgib_Wil2025_COI	DRWA-100	1	BE11648_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	CCCCTCTTTACAAGTTCTTTAGGGT	130.87492
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11649_Asen_Wil2025_COI	DRWA-101	1	BE11649_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	GTTGTTGTTACAACACATGCTCTTTTG	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11649_Cjap_Sim2018_COI	DRWA-101	1	BE11649_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11649_Esin_And2018_Cyb	DRWA-101	1	BE11649_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-101	Cytb	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	ACCCCTCCTCATATCCAACCA	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11649_Mgig_Bot2012_COI	DRWA-101	1	BE11649_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	TCTTATTCGTTGGAGACTTTATAACCCT	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11649_Mjap_Gib2022_COI	DRWA-101	1	BE11649_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	AATTTAATTGTGACTAGGCATGGTCTG	130.87622
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11651_Aamu_Bax2006_COI	DRWA-103	1	BE11651_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	GCACAACCGGGATCTTTACTTC	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11651_Pcan_Bot2011_IGS	DRWA-103	1	BE11651_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-103	IGS	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	CGTAATCCTCAGTACTGGCTA	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11651_Pper_Dia2013_COI	DRWA-103	1	BE11651_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	CTTAGTGGCATTAATTCGDAATCC	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11651_Sspa_Oph2007_COI	DRWA-103	1	BE11651_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	GAAGGCCTTCCGATGATC	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11651_Upin_Bot2011_COI	DRWA-103	1	BE11651_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	CTTTAATTACAGCGTTTTTATTGTTGT	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11651_Vgib_Wil2025_COI	DRWA-103	1	BE11651_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	CCCCTCTTTACAAGTTCTTTAGGGT	130.87559
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11652_Asen_Wil2025_COI	DRWA-104	1	BE11652_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	GTTGTTGTTACAACACATGCTCTTTTG	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11652_Cjap_Sim2018_COI	DRWA-104	1	BE11652_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11652_Esin_And2018_Cyb	DRWA-104	1	BE11652_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-104	Cytb	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	ACCCCTCCTCATATCCAACCA	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11652_Mgig_Bot2012_COI	DRWA-104	1	BE11652_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	TCTTATTCGTTGGAGACTTTATAACCCT	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11652_Mjap_Gib2022_COI	DRWA-104	1	BE11652_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	AATTTAATTGTGACTAGGCATGGTCTG	130.87494
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11653_Cmae_Bot2011_COI	DRWA-105	1	BE11653_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	ATGAACAGTCTATCCTCCTTTAG	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11653_Hsan_Wil2021_COI	DRWA-105	1	BE11653_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	CCTGGGCCGGTATAGTAGGT	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11653_Mros_Gib2022_COI	DRWA-105	1	BE11653_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11653_Msal_Bot2012_COI	DRWA-105	1	BE11653_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	GYTAGTTCCRATGATGTTAGCTG	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11653_Mstr_Wil2021_COI	DRWA-105	1	BE11653_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	GTAGTGGTAACTACTCATGCTTTA	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11653_Pvir_Dia2013_COI	DRWA-105	1	BE11653_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	CTTAGTGGCATTAATTCGDAATCC	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11653_Rhar_Sim2018_COI	DRWA-105	1	BE11653_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	CTGCTGCTATTGCTCATG	130.87621
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11798_Aamu_Bax2006_COI	DRWB-101	1	BE11798_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	GCACAACCGGGATCTTTACTTC	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11798_Pcan_Bot2011_IGS	DRWB-101	1	BE11798_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-101	IGS	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	CGTAATCCTCAGTACTGGCTA	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11798_Pper_Dia2013_COI	DRWB-101	1	BE11798_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	CTTAGTGGCATTAATTCGDAATCC	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11798_Sspa_Oph2007_COI	DRWB-101	1	BE11798_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	GAAGGCCTTCCGATGATC	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11798_Upin_Bot2011_COI	DRWB-101	1	BE11798_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	CTTTAATTACAGCGTTTTTATTGTTGT	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11798_Vgib_Wil2025_COI	DRWB-101	1	BE11798_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	CCCCTCTTTACAAGTTCTTTAGGGT	130.9282
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11799_Asen_Wil2025_COI	DRWB-102	1	BE11799_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	GTTGTTGTTACAACACATGCTCTTTTG	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11799_Cjap_Sim2018_COI	DRWB-102	1	BE11799_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11799_Esin_And2018_Cyb	DRWB-102	1	BE11799_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-102	Cytb	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	ACCCCTCCTCATATCCAACCA	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11799_Mgig_Bot2012_COI	DRWB-102	1	BE11799_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	TCTTATTCGTTGGAGACTTTATAACCCT	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11799_Mjap_Gib2022_COI	DRWB-102	1	BE11799_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	AATTTAATTGTGACTAGGCATGGTCTG	130.9292
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11800_Cmae_Bot2011_COI	DRWB-103	1	BE11800_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	ATGAACAGTCTATCCTCCTTTAG	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11800_Hsan_Wil2021_COI	DRWB-103	1	BE11800_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	CCTGGGCCGGTATAGTAGGT	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11800_Mros_Gib2022_COI	DRWB-103	1	BE11800_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11800_Msal_Bot2012_COI	DRWB-103	1	BE11800_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	GYTAGTTCCRATGATGTTAGCTG	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11800_Mstr_Wil2021_COI	DRWB-103	1	BE11800_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	GTAGTGGTAACTACTCATGCTTTA	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11800_Pvir_Dia2013_COI	DRWB-103	1	BE11800_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	CTTAGTGGCATTAATTCGDAATCC	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11800_Rhar_Sim2018_COI	DRWB-103	1	BE11800_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	CTGCTGCTATTGCTCATG	130.93014
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11802_Aamu_Bax2006_COI	DRWB-105	1	BE11802_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	GCACAACCGGGATCTTTACTTC	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11802_Pcan_Bot2011_IGS	DRWB-105	1	BE11802_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-105	IGS	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	CGTAATCCTCAGTACTGGCTA	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11802_Pper_Dia2013_COI	DRWB-105	1	BE11802_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	CTTAGTGGCATTAATTCGDAATCC	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11802_Sspa_Oph2007_COI	DRWB-105	1	BE11802_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	GAAGGCCTTCCGATGATC	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11802_Upin_Bot2011_COI	DRWB-105	1	BE11802_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	CTTTAATTACAGCGTTTTTATTGTTGT	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11802_Vgib_Wil2025_COI	DRWB-105	1	BE11802_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	CCCCTCTTTACAAGTTCTTTAGGGT	130.92863
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11803_Asen_Wil2025_COI	DRWB-106	1	BE11803_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	GTTGTTGTTACAACACATGCTCTTTTG	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11803_Cjap_Sim2018_COI	DRWB-106	1	BE11803_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11803_Esin_And2018_Cyb	DRWB-106	1	BE11803_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-106	Cytb	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	ACCCCTCCTCATATCCAACCA	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11803_Mgig_Bot2012_COI	DRWB-106	1	BE11803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	TCTTATTCGTTGGAGACTTTATAACCCT	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11803_Mjap_Gib2022_COI	DRWB-106	1	BE11803_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	AATTTAATTGTGACTAGGCATGGTCTG	130.88186
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11804_Cmae_Bot2011_COI	DRWB-107	1	BE11804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	ATGAACAGTCTATCCTCCTTTAG	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11804_Hsan_Wil2021_COI	DRWB-107	1	BE11804_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	CCTGGGCCGGTATAGTAGGT	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11804_Mros_Gib2022_COI	DRWB-107	1	BE11804_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11804_Msal_Bot2012_COI	DRWB-107	1	BE11804_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	GYTAGTTCCRATGATGTTAGCTG	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11804_Mstr_Wil2021_COI	DRWB-107	1	BE11804_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	GTAGTGGTAACTACTCATGCTTTA	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11804_Pvir_Dia2013_COI	DRWB-107	1	BE11804_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	CTTAGTGGCATTAATTCGDAATCC	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11804_Rhar_Sim2018_COI	DRWB-107	1	BE11804_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	CTGCTGCTATTGCTCATG	130.88878
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11806_Aamu_Bax2006_COI	DRWB-109	1	BE11806_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	GCACAACCGGGATCTTTACTTC	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11806_Pcan_Bot2011_IGS	DRWB-109	1	BE11806_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-109	IGS	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	CGTAATCCTCAGTACTGGCTA	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11806_Pper_Dia2013_COI	DRWB-109	1	BE11806_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	CTTAGTGGCATTAATTCGDAATCC	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11806_Sspa_Oph2007_COI	DRWB-109	1	BE11806_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	GAAGGCCTTCCGATGATC	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11806_Upin_Bot2011_COI	DRWB-109	1	BE11806_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	CTTTAATTACAGCGTTTTTATTGTTGT	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11806_Vgib_Wil2025_COI	DRWB-109	1	BE11806_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	CCCCTCTTTACAAGTTCTTTAGGGT	130.89198
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11807_Asen_Wil2025_COI	DRWB-110	1	BE11807_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	GTTGTTGTTACAACACATGCTCTTTTG	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11807_Cjap_Sim2018_COI	DRWB-110	1	BE11807_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11807_Esin_And2018_Cyb	DRWB-110	1	BE11807_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-110	Cytb	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	ACCCCTCCTCATATCCAACCA	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11807_Mgig_Bot2012_COI	DRWB-110	1	BE11807_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	TCTTATTCGTTGGAGACTTTATAACCCT	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11807_Mjap_Gib2022_COI	DRWB-110	1	BE11807_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	AATTTAATTGTGACTAGGCATGGTCTG	130.87946
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11808_Cmae_Bot2011_COI	DRWB-111	1	BE11808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	ATGAACAGTCTATCCTCCTTTAG	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11808_Hsan_Wil2021_COI	DRWB-111	1	BE11808_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	CCTGGGCCGGTATAGTAGGT	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11808_Mros_Gib2022_COI	DRWB-111	1	BE11808_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11808_Msal_Bot2012_COI	DRWB-111	1	BE11808_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	GYTAGTTCCRATGATGTTAGCTG	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11808_Mstr_Wil2021_COI	DRWB-111	1	BE11808_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	GTAGTGGTAACTACTCATGCTTTA	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11808_Pvir_Dia2013_COI	DRWB-111	1	BE11808_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	CTTAGTGGCATTAATTCGDAATCC	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11808_Rhar_Sim2018_COI	DRWB-111	1	BE11808_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	CTGCTGCTATTGCTCATG	130.88104
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11810_Asen_Wil2025_COI	DRWB-113	1	BE11810_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	GTTGTTGTTACAACACATGCTCTTTTG	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11810_Cjap_Sim2018_COI	DRWB-113	1	BE11810_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11810_Esin_And2018_Cyb	DRWB-113	1	BE11810_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-113	Cytb	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	ACCCCTCCTCATATCCAACCA	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11810_Mgig_Bot2012_COI	DRWB-113	1	BE11810_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	TCTTATTCGTTGGAGACTTTATAACCCT	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11810_Mjap_Gib2022_COI	DRWB-113	1	BE11810_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	AATTTAATTGTGACTAGGCATGGTCTG	130.88405
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11811_Cmae_Bot2011_COI	DRWB-114	1	BE11811_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	ATGAACAGTCTATCCTCCTTTAG	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11811_Hsan_Wil2021_COI	DRWB-114	1	BE11811_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	CCTGGGCCGGTATAGTAGGT	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11811_Mros_Gib2022_COI	DRWB-114	1	BE11811_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11811_Msal_Bot2012_COI	DRWB-114	1	BE11811_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	GYTAGTTCCRATGATGTTAGCTG	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11811_Mstr_Wil2021_COI	DRWB-114	1	BE11811_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	GTAGTGGTAACTACTCATGCTTTA	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11811_Pvir_Dia2013_COI	DRWB-114	1	BE11811_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	CTTAGTGGCATTAATTCGDAATCC	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11811_Rhar_Sim2018_COI	DRWB-114	1	BE11811_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	CTGCTGCTATTGCTCATG	130.88842
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11813_Aamu_Bax2006_COI	DRWB-116	1	BE11813_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	GCACAACCGGGATCTTTACTTC	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11813_Pcan_Bot2011_IGS	DRWB-116	1	BE11813_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-116	IGS	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	CGTAATCCTCAGTACTGGCTA	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11813_Pper_Dia2013_COI	DRWB-116	1	BE11813_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	CTTAGTGGCATTAATTCGDAATCC	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11813_Sspa_Oph2007_COI	DRWB-116	1	BE11813_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	GAAGGCCTTCCGATGATC	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11813_Upin_Bot2011_COI	DRWB-116	1	BE11813_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	CTTTAATTACAGCGTTTTTATTGTTGT	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11813_Vgib_Wil2025_COI	DRWB-116	1	BE11813_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	CCCCTCTTTACAAGTTCTTTAGGGT	130.87898
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11814_Asen_Wil2025_COI	DRWB-117	1	BE11814_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	GTTGTTGTTACAACACATGCTCTTTTG	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11814_Cjap_Sim2018_COI	DRWB-117	1	BE11814_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11814_Esin_And2018_Cyb	DRWB-117	1	BE11814_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-117	Cytb	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	ACCCCTCCTCATATCCAACCA	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11814_Mgig_Bot2012_COI	DRWB-117	1	BE11814_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	TCTTATTCGTTGGAGACTTTATAACCCT	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11814_Mjap_Gib2022_COI	DRWB-117	1	BE11814_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	AATTTAATTGTGACTAGGCATGGTCTG	130.88021
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11815_Cmae_Bot2011_COI	DRWB-118	1	BE11815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	ATGAACAGTCTATCCTCCTTTAG	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11815_Hsan_Wil2021_COI	DRWB-118	1	BE11815_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	CCTGGGCCGGTATAGTAGGT	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11815_Mros_Gib2022_COI	DRWB-118	1	BE11815_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11815_Msal_Bot2012_COI	DRWB-118	1	BE11815_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	GYTAGTTCCRATGATGTTAGCTG	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11815_Mstr_Wil2021_COI	DRWB-118	1	BE11815_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	GTAGTGGTAACTACTCATGCTTTA	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11815_Pvir_Dia2013_COI	DRWB-118	1	BE11815_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	CTTAGTGGCATTAATTCGDAATCC	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11815_Rhar_Sim2018_COI	DRWB-118	1	BE11815_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	CTGCTGCTATTGCTCATG	130.88173
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11817_Aamu_Bax2006_COI	DRWB-120	1	BE11817_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	GCACAACCGGGATCTTTACTTC	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11817_Pcan_Bot2011_IGS	DRWB-120	1	BE11817_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-120	IGS	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	CGTAATCCTCAGTACTGGCTA	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11817_Pper_Dia2013_COI	DRWB-120	1	BE11817_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	CTTAGTGGCATTAATTCGDAATCC	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11817_Sspa_Oph2007_COI	DRWB-120	1	BE11817_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	GAAGGCCTTCCGATGATC	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11817_Upin_Bot2011_COI	DRWB-120	1	BE11817_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	CTTTAATTACAGCGTTTTTATTGTTGT	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11817_Vgib_Wil2025_COI	DRWB-120	1	BE11817_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	CCCCTCTTTACAAGTTCTTTAGGGT	130.87813
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11818_Asen_Wil2025_COI	DRWB-121	1	BE11818_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	GTTGTTGTTACAACACATGCTCTTTTG	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11818_Cjap_Sim2018_COI	DRWB-121	1	BE11818_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11818_Esin_And2018_Cyb	DRWB-121	1	BE11818_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-121	Cytb	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	ACCCCTCCTCATATCCAACCA	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11818_Mgig_Bot2012_COI	DRWB-121	1	BE11818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	TCTTATTCGTTGGAGACTTTATAACCCT	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11818_Mjap_Gib2022_COI	DRWB-121	1	BE11818_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	AATTTAATTGTGACTAGGCATGGTCTG	130.87964
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11819_Cmae_Bot2011_COI	DRWB-122	1	BE11819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	ATGAACAGTCTATCCTCCTTTAG	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11819_Hsan_Wil2021_COI	DRWB-122	1	BE11819_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	CCTGGGCCGGTATAGTAGGT	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11819_Mros_Gib2022_COI	DRWB-122	1	BE11819_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11819_Msal_Bot2012_COI	DRWB-122	1	BE11819_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	GYTAGTTCCRATGATGTTAGCTG	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11819_Mstr_Wil2021_COI	DRWB-122	1	BE11819_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	GTAGTGGTAACTACTCATGCTTTA	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11819_Pvir_Dia2013_COI	DRWB-122	1	BE11819_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	CTTAGTGGCATTAATTCGDAATCC	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11819_Rhar_Sim2018_COI	DRWB-122	1	BE11819_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	CTGCTGCTATTGCTCATG	130.88122
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11820_Aamu_Bax2006_COI	DRWB-123	1	BE11820_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	GCACAACCGGGATCTTTACTTC	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11820_Pcan_Bot2011_IGS	DRWB-123	1	BE11820_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-123	IGS	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	CGTAATCCTCAGTACTGGCTA	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11820_Pper_Dia2013_COI	DRWB-123	1	BE11820_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	CTTAGTGGCATTAATTCGDAATCC	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11820_Sspa_Oph2007_COI	DRWB-123	1	BE11820_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	GAAGGCCTTCCGATGATC	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11820_Upin_Bot2011_COI	DRWB-123	1	BE11820_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	CTTTAATTACAGCGTTTTTATTGTTGT	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11820_Vgib_Wil2025_COI	DRWB-123	1	BE11820_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	CCCCTCTTTACAAGTTCTTTAGGGT	130.87459
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11821_Asen_Wil2025_COI	DRWB-124	1	BE11821_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	GTTGTTGTTACAACACATGCTCTTTTG	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11821_Cjap_Sim2018_COI	DRWB-124	1	BE11821_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11821_Esin_And2018_Cyb	DRWB-124	1	BE11821_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-124	Cytb	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	ACCCCTCCTCATATCCAACCA	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11821_Mgig_Bot2012_COI	DRWB-124	1	BE11821_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	TCTTATTCGTTGGAGACTTTATAACCCT	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11821_Mjap_Gib2022_COI	DRWB-124	1	BE11821_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	AATTTAATTGTGACTAGGCATGGTCTG	130.87608
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11822_Cmae_Bot2011_COI	DRWB-125	1	BE11822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	ATGAACAGTCTATCCTCCTTTAG	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11822_Hsan_Wil2021_COI	DRWB-125	1	BE11822_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	CCTGGGCCGGTATAGTAGGT	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11822_Mros_Gib2022_COI	DRWB-125	1	BE11822_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11822_Msal_Bot2012_COI	DRWB-125	1	BE11822_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	GYTAGTTCCRATGATGTTAGCTG	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11822_Mstr_Wil2021_COI	DRWB-125	1	BE11822_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	GTAGTGGTAACTACTCATGCTTTA	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11822_Pvir_Dia2013_COI	DRWB-125	1	BE11822_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	CTTAGTGGCATTAATTCGDAATCC	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11822_Rhar_Sim2018_COI	DRWB-125	1	BE11822_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	CTGCTGCTATTGCTCATG	130.87747
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11824_Aamu_Bax2006_COI	DRWB-127	1	BE11824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	GCACAACCGGGATCTTTACTTC	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11824_Pcan_Bot2011_IGS	DRWB-127	1	BE11824_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-127	IGS	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	CGTAATCCTCAGTACTGGCTA	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11824_Pper_Dia2013_COI	DRWB-127	1	BE11824_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	CTTAGTGGCATTAATTCGDAATCC	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11824_Sspa_Oph2007_COI	DRWB-127	1	BE11824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	GAAGGCCTTCCGATGATC	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11824_Upin_Bot2011_COI	DRWB-127	1	BE11824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	CTTTAATTACAGCGTTTTTATTGTTGT	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11824_Vgib_Wil2025_COI	DRWB-127	1	BE11824_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	CCCCTCTTTACAAGTTCTTTAGGGT	130.87409
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11825_Asen_Wil2025_COI	DRWB-128	1	BE11825_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	GTTGTTGTTACAACACATGCTCTTTTG	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11825_Cjap_Sim2018_COI	DRWB-128	1	BE11825_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11825_Esin_And2018_Cyb	DRWB-128	1	BE11825_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-128	Cytb	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	ACCCCTCCTCATATCCAACCA	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11825_Mjap_Gib2022_COI	DRWB-128	1	BE11825_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	AATTTAATTGTGACTAGGCATGGTCTG	130.87545
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11826_Cmae_Bot2011_COI	DRWB-129	1	BE11826_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	ATGAACAGTCTATCCTCCTTTAG	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11826_Hsan_Wil2021_COI	DRWB-129	1	BE11826_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	CCTGGGCCGGTATAGTAGGT	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11826_Mros_Gib2022_COI	DRWB-129	1	BE11826_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11826_Msal_Bot2012_COI	DRWB-129	1	BE11826_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	GYTAGTTCCRATGATGTTAGCTG	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11826_Mstr_Wil2021_COI	DRWB-129	1	BE11826_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	GTAGTGGTAACTACTCATGCTTTA	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11826_Pvir_Dia2013_COI	DRWB-129	1	BE11826_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	CTTAGTGGCATTAATTCGDAATCC	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11826_Rhar_Sim2018_COI	DRWB-129	1	BE11826_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	CTGCTGCTATTGCTCATG	130.87695
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11828_Aamu_Bax2006_COI	DRWB-131	1	BE11828_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	GCACAACCGGGATCTTTACTTC	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11828_Pcan_Bot2011_IGS	DRWB-131	1	BE11828_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-131	IGS	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	CGTAATCCTCAGTACTGGCTA	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11828_Pper_Dia2013_COI	DRWB-131	1	BE11828_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	CTTAGTGGCATTAATTCGDAATCC	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11828_Sspa_Oph2007_COI	DRWB-131	1	BE11828_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	GAAGGCCTTCCGATGATC	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11828_Upin_Bot2011_COI	DRWB-131	1	BE11828_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	CTTTAATTACAGCGTTTTTATTGTTGT	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11828_Vgib_Wil2025_COI	DRWB-131	1	BE11828_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	CCCCTCTTTACAAGTTCTTTAGGGT	130.87468
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11829_Asen_Wil2025_COI	DRWB-132	1	BE11829_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	GTTGTTGTTACAACACATGCTCTTTTG	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11829_Cjap_Sim2018_COI	DRWB-132	1	BE11829_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11829_Esin_And2018_Cyb	DRWB-132	1	BE11829_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-132	Cytb	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	ACCCCTCCTCATATCCAACCA	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11829_Mgig_Bot2012_COI	DRWB-132	1	BE11829_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	TCTTATTCGTTGGAGACTTTATAACCCT	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11829_Mjap_Gib2022_COI	DRWB-132	1	BE11829_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	AATTTAATTGTGACTAGGCATGGTCTG	130.87609
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11831_Aamu_Bax2006_COI	DRWB-134	1	BE11831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	GCACAACCGGGATCTTTACTTC	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11831_Pcan_Bot2011_IGS	DRWB-134	1	BE11831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-134	IGS	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	CGTAATCCTCAGTACTGGCTA	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11831_Pper_Dia2013_COI	DRWB-134	1	BE11831_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	CTTAGTGGCATTAATTCGDAATCC	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11831_Sspa_Oph2007_COI	DRWB-134	1	BE11831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	GAAGGCCTTCCGATGATC	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11831_Upin_Bot2011_COI	DRWB-134	1	BE11831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	CTTTAATTACAGCGTTTTTATTGTTGT	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11831_Vgib_Wil2025_COI	DRWB-134	1	BE11831_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	CCCCTCTTTACAAGTTCTTTAGGGT	130.87337
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11832_Asen_Wil2025_COI	DRWB-135	1	BE11832_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	GTTGTTGTTACAACACATGCTCTTTTG	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11832_Cjap_Sim2018_COI	DRWB-135	1	BE11832_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11832_Esin_And2018_Cyb	DRWB-135	1	BE11832_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-135	Cytb	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	ACCCCTCCTCATATCCAACCA	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11832_Mgig_Bot2012_COI	DRWB-135	1	BE11832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	TCTTATTCGTTGGAGACTTTATAACCCT	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11832_Mjap_Gib2022_COI	DRWB-135	1	BE11832_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	AATTTAATTGTGACTAGGCATGGTCTG	130.87487
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11876_Asen_Wil2025_COI	DRWB-071	1	BE11876_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	GTTGTTGTTACAACACATGCTCTTTTG	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11876_Cjap_Sim2018_COI	DRWB-071	1	BE11876_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11876_Esin_And2018_Cyb	DRWB-071	1	BE11876_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-071	Cytb	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	ACCCCTCCTCATATCCAACCA	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11876_Mgig_Bot2012_COI	DRWB-071	1	BE11876_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	TCTTATTCGTTGGAGACTTTATAACCCT	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11876_Mjap_Gib2022_COI	DRWB-071	1	BE11876_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	AATTTAATTGTGACTAGGCATGGTCTG	130.93133
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11877_Cmae_Bot2011_COI	DRWB-072	1	BE11877_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	ATGAACAGTCTATCCTCCTTTAG	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11877_Hsan_Wil2021_COI	DRWB-072	1	BE11877_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	CCTGGGCCGGTATAGTAGGT	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11877_Mros_Gib2022_COI	DRWB-072	1	BE11877_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11877_Msal_Bot2012_COI	DRWB-072	1	BE11877_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	GYTAGTTCCRATGATGTTAGCTG	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11877_Mstr_Wil2021_COI	DRWB-072	1	BE11877_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	GTAGTGGTAACTACTCATGCTTTA	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11877_Pvir_Dia2013_COI	DRWB-072	1	BE11877_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	CTTAGTGGCATTAATTCGDAATCC	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11877_Rhar_Sim2018_COI	DRWB-072	1	BE11877_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	CTGCTGCTATTGCTCATG	130.93003
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11879_Aamu_Bax2006_COI	DRWB-074	1	BE11879_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	GCACAACCGGGATCTTTACTTC	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11879_Pcan_Bot2011_IGS	DRWB-074	1	BE11879_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-074	IGS	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	CGTAATCCTCAGTACTGGCTA	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11879_Pper_Dia2013_COI	DRWB-074	1	BE11879_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	CTTAGTGGCATTAATTCGDAATCC	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11879_Sspa_Oph2007_COI	DRWB-074	1	BE11879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	GAAGGCCTTCCGATGATC	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11879_Upin_Bot2011_COI	DRWB-074	1	BE11879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	CTTTAATTACAGCGTTTTTATTGTTGT	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11879_Vgib_Wil2025_COI	DRWB-074	1	BE11879_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	CCCCTCTTTACAAGTTCTTTAGGGT	130.93201
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11880_Cmae_Bot2011_COI	DRWB-075	1	BE11880_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	ATGAACAGTCTATCCTCCTTTAG	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11880_Hsan_Wil2021_COI	DRWB-075	1	BE11880_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	CCTGGGCCGGTATAGTAGGT	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11880_Mros_Gib2022_COI	DRWB-075	1	BE11880_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11880_Msal_Bot2012_COI	DRWB-075	1	BE11880_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	GYTAGTTCCRATGATGTTAGCTG	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11880_Mstr_Wil2021_COI	DRWB-075	1	BE11880_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	GTAGTGGTAACTACTCATGCTTTA	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11880_Pvir_Dia2013_COI	DRWB-075	1	BE11880_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	CTTAGTGGCATTAATTCGDAATCC	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11880_Rhar_Sim2018_COI	DRWB-075	1	BE11880_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	CTGCTGCTATTGCTCATG	130.93056
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11882_Aamu_Bax2006_COI	DRWB-077	1	BE11882_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	GCACAACCGGGATCTTTACTTC	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11882_Pcan_Bot2011_IGS	DRWB-077	1	BE11882_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-077	IGS	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	CGTAATCCTCAGTACTGGCTA	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11882_Pper_Dia2013_COI	DRWB-077	1	BE11882_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	CTTAGTGGCATTAATTCGDAATCC	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11882_Sspa_Oph2007_COI	DRWB-077	1	BE11882_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	GAAGGCCTTCCGATGATC	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11882_Upin_Bot2011_COI	DRWB-077	1	BE11882_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	CTTTAATTACAGCGTTTTTATTGTTGT	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11882_Vgib_Wil2025_COI	DRWB-077	1	BE11882_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	CCCCTCTTTACAAGTTCTTTAGGGT	130.93254
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11883_Asen_Wil2025_COI	DRWB-078	1	BE11883_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	GTTGTTGTTACAACACATGCTCTTTTG	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11883_Cjap_Sim2018_COI	DRWB-078	1	BE11883_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11883_Esin_And2018_Cyb	DRWB-078	1	BE11883_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-078	Cytb	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	ACCCCTCCTCATATCCAACCA	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11883_Mgig_Bot2012_COI	DRWB-078	1	BE11883_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	TCTTATTCGTTGGAGACTTTATAACCCT	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11883_Mjap_Gib2022_COI	DRWB-078	1	BE11883_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	AATTTAATTGTGACTAGGCATGGTCTG	130.9301
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11884_Cmae_Bot2011_COI	DRWB-079	1	BE11884_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	ATGAACAGTCTATCCTCCTTTAG	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11884_Hsan_Wil2021_COI	DRWB-079	1	BE11884_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	CCTGGGCCGGTATAGTAGGT	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11884_Mros_Gib2022_COI	DRWB-079	1	BE11884_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11884_Msal_Bot2012_COI	DRWB-079	1	BE11884_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	GYTAGTTCCRATGATGTTAGCTG	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11884_Mstr_Wil2021_COI	DRWB-079	1	BE11884_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	GTAGTGGTAACTACTCATGCTTTA	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11884_Pvir_Dia2013_COI	DRWB-079	1	BE11884_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	CTTAGTGGCATTAATTCGDAATCC	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11884_Rhar_Sim2018_COI	DRWB-079	1	BE11884_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	CTGCTGCTATTGCTCATG	130.93108
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11886_Aamu_Bax2006_COI	DRWB-081	1	BE11886_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	GCACAACCGGGATCTTTACTTC	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11886_Pcan_Bot2011_IGS	DRWB-081	1	BE11886_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-081	IGS	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	CGTAATCCTCAGTACTGGCTA	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11886_Pper_Dia2013_COI	DRWB-081	1	BE11886_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	CTTAGTGGCATTAATTCGDAATCC	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11886_Sspa_Oph2007_COI	DRWB-081	1	BE11886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	GAAGGCCTTCCGATGATC	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11886_Upin_Bot2011_COI	DRWB-081	1	BE11886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	CTTTAATTACAGCGTTTTTATTGTTGT	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11886_Vgib_Wil2025_COI	DRWB-081	1	BE11886_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	CCCCTCTTTACAAGTTCTTTAGGGT	130.92961
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11887_Asen_Wil2025_COI	DRWB-082	1	BE11887_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	GTTGTTGTTACAACACATGCTCTTTTG	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11887_Cjap_Sim2018_COI	DRWB-082	1	BE11887_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11887_Esin_And2018_Cyb	DRWB-082	1	BE11887_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-082	Cytb	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	ACCCCTCCTCATATCCAACCA	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11887_Mgig_Bot2012_COI	DRWB-082	1	BE11887_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	TCTTATTCGTTGGAGACTTTATAACCCT	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11887_Mjap_Gib2022_COI	DRWB-082	1	BE11887_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	AATTTAATTGTGACTAGGCATGGTCTG	130.93061
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11888_Cmae_Bot2011_COI	DRWB-083	1	BE11888_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	ATGAACAGTCTATCCTCCTTTAG	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11888_Hsan_Wil2021_COI	DRWB-083	1	BE11888_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	CCTGGGCCGGTATAGTAGGT	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11888_Mros_Gib2022_COI	DRWB-083	1	BE11888_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11888_Msal_Bot2012_COI	DRWB-083	1	BE11888_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	GYTAGTTCCRATGATGTTAGCTG	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11888_Mstr_Wil2021_COI	DRWB-083	1	BE11888_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	GTAGTGGTAACTACTCATGCTTTA	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11888_Pvir_Dia2013_COI	DRWB-083	1	BE11888_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	CTTAGTGGCATTAATTCGDAATCC	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11888_Rhar_Sim2018_COI	DRWB-083	1	BE11888_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	CTGCTGCTATTGCTCATG	130.9316
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11890_Asen_Wil2025_COI	DRWB-085	1	BE11890_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	GTTGTTGTTACAACACATGCTCTTTTG	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11890_Cjap_Sim2018_COI	DRWB-085	1	BE11890_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11890_Esin_And2018_Cyb	DRWB-085	1	BE11890_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-085	Cytb	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	ACCCCTCCTCATATCCAACCA	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11890_Mgig_Bot2012_COI	DRWB-085	1	BE11890_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	TCTTATTCGTTGGAGACTTTATAACCCT	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11890_Mjap_Gib2022_COI	DRWB-085	1	BE11890_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	AATTTAATTGTGACTAGGCATGGTCTG	130.93012
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11891_Cmae_Bot2011_COI	DRWB-086	1	BE11891_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	ATGAACAGTCTATCCTCCTTTAG	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11891_Hsan_Wil2021_COI	DRWB-086	1	BE11891_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	CCTGGGCCGGTATAGTAGGT	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11891_Mros_Gib2022_COI	DRWB-086	1	BE11891_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11891_Msal_Bot2012_COI	DRWB-086	1	BE11891_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	GYTAGTTCCRATGATGTTAGCTG	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11891_Mstr_Wil2021_COI	DRWB-086	1	BE11891_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	GTAGTGGTAACTACTCATGCTTTA	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11891_Pvir_Dia2013_COI	DRWB-086	1	BE11891_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	CTTAGTGGCATTAATTCGDAATCC	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11891_Rhar_Sim2018_COI	DRWB-086	1	BE11891_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	CTGCTGCTATTGCTCATG	130.93112
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11893_Aamu_Bax2006_COI	DRWB-088	1	BE11893_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	GCACAACCGGGATCTTTACTTC	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11893_Pcan_Bot2011_IGS	DRWB-088	1	BE11893_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-088	IGS	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	CGTAATCCTCAGTACTGGCTA	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11893_Pper_Dia2013_COI	DRWB-088	1	BE11893_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	CTTAGTGGCATTAATTCGDAATCC	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11893_Sspa_Oph2007_COI	DRWB-088	1	BE11893_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	GAAGGCCTTCCGATGATC	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11893_Upin_Bot2011_COI	DRWB-088	1	BE11893_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	CTTTAATTACAGCGTTTTTATTGTTGT	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11893_Vgib_Wil2025_COI	DRWB-088	1	BE11893_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	CCCCTCTTTACAAGTTCTTTAGGGT	130.92859
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11894_Asen_Wil2025_COI	DRWB-089	1	BE11894_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	GTTGTTGTTACAACACATGCTCTTTTG	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11894_Cjap_Sim2018_COI	DRWB-089	1	BE11894_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11894_Esin_And2018_Cyb	DRWB-089	1	BE11894_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-089	Cytb	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	ACCCCTCCTCATATCCAACCA	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11894_Mgig_Bot2012_COI	DRWB-089	1	BE11894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	TCTTATTCGTTGGAGACTTTATAACCCT	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11894_Mjap_Gib2022_COI	DRWB-089	1	BE11894_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	AATTTAATTGTGACTAGGCATGGTCTG	130.92963
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11895_Cmae_Bot2011_COI	DRWB-090	1	BE11895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	ATGAACAGTCTATCCTCCTTTAG	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11895_Hsan_Wil2021_COI	DRWB-090	1	BE11895_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	CCTGGGCCGGTATAGTAGGT	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11895_Mros_Gib2022_COI	DRWB-090	1	BE11895_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11895_Msal_Bot2012_COI	DRWB-090	1	BE11895_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	GYTAGTTCCRATGATGTTAGCTG	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11895_Mstr_Wil2021_COI	DRWB-090	1	BE11895_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	GTAGTGGTAACTACTCATGCTTTA	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11895_Pvir_Dia2013_COI	DRWB-090	1	BE11895_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	CTTAGTGGCATTAATTCGDAATCC	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11895_Rhar_Sim2018_COI	DRWB-090	1	BE11895_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	CTGCTGCTATTGCTCATG	130.93065
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11897_Aamu_Bax2006_COI	DRWB-092	1	BE11897_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	GCACAACCGGGATCTTTACTTC	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11897_Pcan_Bot2011_IGS	DRWB-092	1	BE11897_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-092	IGS	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	CGTAATCCTCAGTACTGGCTA	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11897_Pper_Dia2013_COI	DRWB-092	1	BE11897_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	CTTAGTGGCATTAATTCGDAATCC	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11897_Sspa_Oph2007_COI	DRWB-092	1	BE11897_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	GAAGGCCTTCCGATGATC	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11897_Upin_Bot2011_COI	DRWB-092	1	BE11897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	CTTTAATTACAGCGTTTTTATTGTTGT	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11897_Vgib_Wil2025_COI	DRWB-092	1	BE11897_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	CCCCTCTTTACAAGTTCTTTAGGGT	130.9281
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11898_Asen_Wil2025_COI	DRWB-093	1	BE11898_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	GTTGTTGTTACAACACATGCTCTTTTG	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11898_Cjap_Sim2018_COI	DRWB-093	1	BE11898_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11898_Esin_And2018_Cyb	DRWB-093	1	BE11898_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-093	Cytb	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	ACCCCTCCTCATATCCAACCA	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11898_Mgig_Bot2012_COI	DRWB-093	1	BE11898_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	TCTTATTCGTTGGAGACTTTATAACCCT	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11898_Mjap_Gib2022_COI	DRWB-093	1	BE11898_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	AATTTAATTGTGACTAGGCATGGTCTG	130.92915
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11899_Cmae_Bot2011_COI	DRWB-094	1	BE11899_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	ATGAACAGTCTATCCTCCTTTAG	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11899_Hsan_Wil2021_COI	DRWB-094	1	BE11899_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	CCTGGGCCGGTATAGTAGGT	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11899_Mros_Gib2022_COI	DRWB-094	1	BE11899_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11899_Msal_Bot2012_COI	DRWB-094	1	BE11899_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	GYTAGTTCCRATGATGTTAGCTG	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11899_Mstr_Wil2021_COI	DRWB-094	1	BE11899_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	GTAGTGGTAACTACTCATGCTTTA	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11899_Pvir_Dia2013_COI	DRWB-094	1	BE11899_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	CTTAGTGGCATTAATTCGDAATCC	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11899_Rhar_Sim2018_COI	DRWB-094	1	BE11899_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	CTGCTGCTATTGCTCATG	130.93013
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11901_Aamu_Bax2006_COI	DRWB-096	1	BE11901_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	GCACAACCGGGATCTTTACTTC	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11901_Pcan_Bot2011_IGS	DRWB-096	1	BE11901_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-096	IGS	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	CGTAATCCTCAGTACTGGCTA	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11901_Pper_Dia2013_COI	DRWB-096	1	BE11901_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	CTTAGTGGCATTAATTCGDAATCC	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11901_Sspa_Oph2007_COI	DRWB-096	1	BE11901_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	GAAGGCCTTCCGATGATC	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11901_Upin_Bot2011_COI	DRWB-096	1	BE11901_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	CTTTAATTACAGCGTTTTTATTGTTGT	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11901_Vgib_Wil2025_COI	DRWB-096	1	BE11901_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	CCCCTCTTTACAAGTTCTTTAGGGT	130.92764
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11902_Asen_Wil2025_COI	DRWB-097	1	BE11902_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	GTTGTTGTTACAACACATGCTCTTTTG	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11902_Cjap_Sim2018_COI	DRWB-097	1	BE11902_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11902_Esin_And2018_Cyb	DRWB-097	1	BE11902_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-097	Cytb	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	ACCCCTCCTCATATCCAACCA	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11902_Mgig_Bot2012_COI	DRWB-097	1	BE11902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	TCTTATTCGTTGGAGACTTTATAACCCT	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11902_Mjap_Gib2022_COI	DRWB-097	1	BE11902_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	AATTTAATTGTGACTAGGCATGGTCTG	130.92861
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11903_Cmae_Bot2011_COI	DRWB-098	1	BE11903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	ATGAACAGTCTATCCTCCTTTAG	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11903_Hsan_Wil2021_COI	DRWB-098	1	BE11903_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	CCTGGGCCGGTATAGTAGGT	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11903_Mros_Gib2022_COI	DRWB-098	1	BE11903_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11903_Msal_Bot2012_COI	DRWB-098	1	BE11903_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	GYTAGTTCCRATGATGTTAGCTG	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11903_Mstr_Wil2021_COI	DRWB-098	1	BE11903_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	GTAGTGGTAACTACTCATGCTTTA	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11903_Pvir_Dia2013_COI	DRWB-098	1	BE11903_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	CTTAGTGGCATTAATTCGDAATCC	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11903_Rhar_Sim2018_COI	DRWB-098	1	BE11903_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	CTGCTGCTATTGCTCATG	130.92962
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11913_Asen_Wil2025_COI	GLTB-006	1	BE11913_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	GTTGTTGTTACAACACATGCTCTTTTG	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11913_Cjap_Sim2018_COI	GLTB-006	1	BE11913_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11913_Esin_And2018_Cyb	GLTB-006	1	BE11913_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-006	Cytb	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	ACCCCTCCTCATATCCAACCA	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11913_Mgig_Bot2012_COI	GLTB-006	1	BE11913_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	TCTTATTCGTTGGAGACTTTATAACCCT	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11913_Mjap_Gib2022_COI	GLTB-006	1	BE11913_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	AATTTAATTGTGACTAGGCATGGTCTG	151.32344
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11914_Cmae_Bot2011_COI	GLTB-018	1	BE11914_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	ATGAACAGTCTATCCTCCTTTAG	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11914_Hsan_Wil2021_COI	GLTB-018	1	BE11914_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	CCTGGGCCGGTATAGTAGGT	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11914_Mros_Gib2022_COI	GLTB-018	1	BE11914_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11914_Msal_Bot2012_COI	GLTB-018	1	BE11914_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	GYTAGTTCCRATGATGTTAGCTG	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11914_Mstr_Wil2021_COI	GLTB-018	1	BE11914_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	GTAGTGGTAACTACTCATGCTTTA	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11914_Pvir_Dia2013_COI	GLTB-018	1	BE11914_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	CTTAGTGGCATTAATTCGDAATCC	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11914_Rhar_Sim2018_COI	GLTB-018	1	BE11914_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	CTGCTGCTATTGCTCATG	151.32254
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11916_Aamu_Bax2006_COI	GLTB-024	1	BE11916_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	GCACAACCGGGATCTTTACTTC	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11916_Pcan_Bot2011_IGS	GLTB-024	1	BE11916_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-024	IGS	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	CGTAATCCTCAGTACTGGCTA	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11916_Pper_Dia2013_COI	GLTB-024	1	BE11916_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	CTTAGTGGCATTAATTCGDAATCC	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11916_Sspa_Oph2007_COI	GLTB-024	1	BE11916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	GAAGGCCTTCCGATGATC	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11916_Upin_Bot2011_COI	GLTB-024	1	BE11916_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	CTTTAATTACAGCGTTTTTATTGTTGT	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11916_Vgib_Wil2025_COI	GLTB-024	1	BE11916_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	CCCCTCTTTACAAGTTCTTTAGGGT	151.31444
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11917_Asen_Wil2025_COI	GLTB-030	1	BE11917_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	GTTGTTGTTACAACACATGCTCTTTTG	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11917_Cjap_Sim2018_COI	GLTB-030	1	BE11917_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11917_Esin_And2018_Cyb	GLTB-030	1	BE11917_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-030	Cytb	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	ACCCCTCCTCATATCCAACCA	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11917_Mgig_Bot2012_COI	GLTB-030	1	BE11917_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	TCTTATTCGTTGGAGACTTTATAACCCT	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11917_Mjap_Gib2022_COI	GLTB-030	1	BE11917_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	AATTTAATTGTGACTAGGCATGGTCTG	151.31018
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11918_Cmae_Bot2011_COI	GLTB-029	1	BE11918_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	ATGAACAGTCTATCCTCCTTTAG	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11918_Mros_Gib2022_COI	GLTB-029	1	BE11918_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11918_Msal_Bot2012_COI	GLTB-029	1	BE11918_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	GYTAGTTCCRATGATGTTAGCTG	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11918_Mstr_Wil2021_COI	GLTB-029	1	BE11918_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	GTAGTGGTAACTACTCATGCTTTA	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11918_Pvir_Dia2013_COI	GLTB-029	1	BE11918_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	CTTAGTGGCATTAATTCGDAATCC	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11918_Rhar_Sim2018_COI	GLTB-029	1	BE11918_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	CTGCTGCTATTGCTCATG	151.30871
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11920_Asen_Wil2025_COI	GLTB-004	1	BE11920_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	GTTGTTGTTACAACACATGCTCTTTTG	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11920_Cjap_Sim2018_COI	GLTB-004	1	BE11920_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11920_Esin_And2018_Cyb	GLTB-004	1	BE11920_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-004	Cytb	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	ACCCCTCCTCATATCCAACCA	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11920_Mgig_Bot2012_COI	GLTB-004	1	BE11920_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	TCTTATTCGTTGGAGACTTTATAACCCT	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11920_Mjap_Gib2022_COI	GLTB-004	1	BE11920_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	AATTTAATTGTGACTAGGCATGGTCTG	151.32434
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11921_Cmae_Bot2011_COI	GLTB-010	1	BE11921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	ATGAACAGTCTATCCTCCTTTAG	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11921_Hsan_Wil2021_COI	GLTB-010	1	BE11921_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	CCTGGGCCGGTATAGTAGGT	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11921_Mros_Gib2022_COI	GLTB-010	1	BE11921_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11921_Msal_Bot2012_COI	GLTB-010	1	BE11921_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	GYTAGTTCCRATGATGTTAGCTG	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11921_Mstr_Wil2021_COI	GLTB-010	1	BE11921_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	GTAGTGGTAACTACTCATGCTTTA	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11921_Pvir_Dia2013_COI	GLTB-010	1	BE11921_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	CTTAGTGGCATTAATTCGDAATCC	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11921_Rhar_Sim2018_COI	GLTB-010	1	BE11921_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	CTGCTGCTATTGCTCATG	151.32044
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11923_Aamu_Bax2006_COI	GLTB-017	1	BE11923_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	GCACAACCGGGATCTTTACTTC	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11923_Pcan_Bot2011_IGS	GLTB-017	1	BE11923_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-017	IGS	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	CGTAATCCTCAGTACTGGCTA	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11923_Pper_Dia2013_COI	GLTB-017	1	BE11923_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	CTTAGTGGCATTAATTCGDAATCC	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11923_Sspa_Oph2007_COI	GLTB-017	1	BE11923_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	GAAGGCCTTCCGATGATC	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11923_Upin_Bot2011_COI	GLTB-017	1	BE11923_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	CTTTAATTACAGCGTTTTTATTGTTGT	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11923_Vgib_Wil2025_COI	GLTB-017	1	BE11923_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	CCCCTCTTTACAAGTTCTTTAGGGT	151.32109
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11924_Asen_Wil2025_COI	GLTB-023	1	BE11924_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	GTTGTTGTTACAACACATGCTCTTTTG	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11924_Cjap_Sim2018_COI	GLTB-023	1	BE11924_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11924_Esin_And2018_Cyb	GLTB-023	1	BE11924_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-023	Cytb	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	ACCCCTCCTCATATCCAACCA	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11924_Mgig_Bot2012_COI	GLTB-023	1	BE11924_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	TCTTATTCGTTGGAGACTTTATAACCCT	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11924_Mjap_Gib2022_COI	GLTB-023	1	BE11924_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	AATTTAATTGTGACTAGGCATGGTCTG	151.31305
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11925_Cmae_Bot2011_COI	GLTB-022	1	BE11925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	ATGAACAGTCTATCCTCCTTTAG	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11925_Hsan_Wil2021_COI	GLTB-022	1	BE11925_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	CCTGGGCCGGTATAGTAGGT	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11925_Mros_Gib2022_COI	GLTB-022	1	BE11925_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11925_Msal_Bot2012_COI	GLTB-022	1	BE11925_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	GYTAGTTCCRATGATGTTAGCTG	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11925_Mstr_Wil2021_COI	GLTB-022	1	BE11925_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	GTAGTGGTAACTACTCATGCTTTA	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11925_Pvir_Dia2013_COI	GLTB-022	1	BE11925_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	CTTAGTGGCATTAATTCGDAATCC	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11925_Rhar_Sim2018_COI	GLTB-022	1	BE11925_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	CTGCTGCTATTGCTCATG	151.31132
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11927_Aamu_Bax2006_COI	GLTB-003	1	BE11927_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	GCACAACCGGGATCTTTACTTC	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11927_Pcan_Bot2011_IGS	GLTB-003	1	BE11927_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-003	IGS	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	CGTAATCCTCAGTACTGGCTA	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11927_Pper_Dia2013_COI	GLTB-003	1	BE11927_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	CTTAGTGGCATTAATTCGDAATCC	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11927_Sspa_Oph2007_COI	GLTB-003	1	BE11927_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	GAAGGCCTTCCGATGATC	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11927_Upin_Bot2011_COI	GLTB-003	1	BE11927_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	CTTTAATTACAGCGTTTTTATTGTTGT	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11927_Vgib_Wil2025_COI	GLTB-003	1	BE11927_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	CCCCTCTTTACAAGTTCTTTAGGGT	151.32561
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11928_Asen_Wil2025_COI	GLTB-028	1	BE11928_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	GTTGTTGTTACAACACATGCTCTTTTG	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11928_Cjap_Sim2018_COI	GLTB-028	1	BE11928_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11928_Esin_And2018_Cyb	GLTB-028	1	BE11928_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-028	Cytb	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	ACCCCTCCTCATATCCAACCA	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11928_Mgig_Bot2012_COI	GLTB-028	1	BE11928_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	TCTTATTCGTTGGAGACTTTATAACCCT	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11928_Mjap_Gib2022_COI	GLTB-028	1	BE11928_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	AATTTAATTGTGACTAGGCATGGTCTG	151.30658
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11929_Cmae_Bot2011_COI	GLTB-009	1	BE11929_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	ATGAACAGTCTATCCTCCTTTAG	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11929_Hsan_Wil2021_COI	GLTB-009	1	BE11929_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	CCTGGGCCGGTATAGTAGGT	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11929_Mros_Gib2022_COI	GLTB-009	1	BE11929_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11929_Msal_Bot2012_COI	GLTB-009	1	BE11929_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	GYTAGTTCCRATGATGTTAGCTG	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11929_Mstr_Wil2021_COI	GLTB-009	1	BE11929_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	GTAGTGGTAACTACTCATGCTTTA	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11929_Pvir_Dia2013_COI	GLTB-009	1	BE11929_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	CTTAGTGGCATTAATTCGDAATCC	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11929_Rhar_Sim2018_COI	GLTB-009	1	BE11929_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	CTGCTGCTATTGCTCATG	151.31877
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11930_Aamu_Bax2006_COI	GLTB-015	1	BE11930_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	GCACAACCGGGATCTTTACTTC	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11930_Pcan_Bot2011_IGS	GLTB-015	1	BE11930_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-015	IGS	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	CGTAATCCTCAGTACTGGCTA	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11930_Pper_Dia2013_COI	GLTB-015	1	BE11930_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	CTTAGTGGCATTAATTCGDAATCC	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11930_Sspa_Oph2007_COI	GLTB-015	1	BE11930_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	GAAGGCCTTCCGATGATC	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11930_Upin_Bot2011_COI	GLTB-015	1	BE11930_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	CTTTAATTACAGCGTTTTTATTGTTGT	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11930_Vgib_Wil2025_COI	GLTB-015	1	BE11930_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	CCCCTCTTTACAAGTTCTTTAGGGT	151.31768
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11931_Asen_Wil2025_COI	GLTB-027	1	BE11931_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	GTTGTTGTTACAACACATGCTCTTTTG	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11931_Cjap_Sim2018_COI	GLTB-027	1	BE11931_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11931_Esin_And2018_Cyb	GLTB-027	1	BE11931_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-027	Cytb	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	ACCCCTCCTCATATCCAACCA	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11931_Mgig_Bot2012_COI	GLTB-027	1	BE11931_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	TCTTATTCGTTGGAGACTTTATAACCCT	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11931_Mjap_Gib2022_COI	GLTB-027	1	BE11931_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	AATTTAATTGTGACTAGGCATGGTCTG	151.31963
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11932_Cmae_Bot2011_COI	GLTB-021	1	BE11932_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	ATGAACAGTCTATCCTCCTTTAG	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11932_Hsan_Wil2021_COI	GLTB-021	1	BE11932_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	CCTGGGCCGGTATAGTAGGT	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11932_Mros_Gib2022_COI	GLTB-021	1	BE11932_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11932_Msal_Bot2012_COI	GLTB-021	1	BE11932_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	GYTAGTTCCRATGATGTTAGCTG	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11932_Mstr_Wil2021_COI	GLTB-021	1	BE11932_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	GTAGTGGTAACTACTCATGCTTTA	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11932_Pvir_Dia2013_COI	GLTB-021	1	BE11932_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	CTTAGTGGCATTAATTCGDAATCC	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11932_Rhar_Sim2018_COI	GLTB-021	1	BE11932_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	CTGCTGCTATTGCTCATG	151.3098
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11934_Aamu_Bax2006_COI	GLTB-008	1	BE11934_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	GCACAACCGGGATCTTTACTTC	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11934_Pcan_Bot2011_IGS	GLTB-008	1	BE11934_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-008	IGS	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	CGTAATCCTCAGTACTGGCTA	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11934_Pper_Dia2013_COI	GLTB-008	1	BE11934_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	CTTAGTGGCATTAATTCGDAATCC	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11934_Sspa_Oph2007_COI	GLTB-008	1	BE11934_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	GAAGGCCTTCCGATGATC	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11934_Upin_Bot2011_COI	GLTB-008	1	BE11934_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	CTTTAATTACAGCGTTTTTATTGTTGT	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11934_Vgib_Wil2025_COI	GLTB-008	1	BE11934_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	CCCCTCTTTACAAGTTCTTTAGGGT	151.31702
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11935_Asen_Wil2025_COI	GLTB-026	1	BE11935_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	GTTGTTGTTACAACACATGCTCTTTTG	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11935_Cjap_Sim2018_COI	GLTB-026	1	BE11935_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11935_Esin_And2018_Cyb	GLTB-026	1	BE11935_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-026	Cytb	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	ACCCCTCCTCATATCCAACCA	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11935_Mgig_Bot2012_COI	GLTB-026	1	BE11935_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	TCTTATTCGTTGGAGACTTTATAACCCT	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11935_Mjap_Gib2022_COI	GLTB-026	1	BE11935_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	AATTTAATTGTGACTAGGCATGGTCTG	151.31817
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11936_Cmae_Bot2011_COI	GLTB-020	1	BE11936_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	ATGAACAGTCTATCCTCCTTTAG	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11936_Hsan_Wil2021_COI	GLTB-020	1	BE11936_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	CCTGGGCCGGTATAGTAGGT	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11936_Mros_Gib2022_COI	GLTB-020	1	BE11936_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11936_Msal_Bot2012_COI	GLTB-020	1	BE11936_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	GYTAGTTCCRATGATGTTAGCTG	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11936_Mstr_Wil2021_COI	GLTB-020	1	BE11936_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	GTAGTGGTAACTACTCATGCTTTA	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11936_Pvir_Dia2013_COI	GLTB-020	1	BE11936_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	CTTAGTGGCATTAATTCGDAATCC	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11936_Rhar_Sim2018_COI	GLTB-020	1	BE11936_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	CTGCTGCTATTGCTCATG	151.30812
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11938_Aamu_Bax2006_COI	GLTB-025	1	BE11938_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	GCACAACCGGGATCTTTACTTC	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11938_Pcan_Bot2011_IGS	GLTB-025	1	BE11938_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-025	IGS	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	CGTAATCCTCAGTACTGGCTA	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11938_Pper_Dia2013_COI	GLTB-025	1	BE11938_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	CTTAGTGGCATTAATTCGDAATCC	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11938_Sspa_Oph2007_COI	GLTB-025	1	BE11938_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	GAAGGCCTTCCGATGATC	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11938_Upin_Bot2011_COI	GLTB-025	1	BE11938_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	CTTTAATTACAGCGTTTTTATTGTTGT	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11938_Vgib_Wil2025_COI	GLTB-025	1	BE11938_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	CCCCTCTTTACAAGTTCTTTAGGGT	151.31631
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11939_Asen_Wil2025_COI	GLTB-007	1	BE11939_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	GTTGTTGTTACAACACATGCTCTTTTG	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11939_Cjap_Sim2018_COI	GLTB-007	1	BE11939_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11939_Esin_And2018_Cyb	GLTB-007	1	BE11939_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-007	Cytb	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	ACCCCTCCTCATATCCAACCA	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11939_Mgig_Bot2012_COI	GLTB-007	1	BE11939_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	TCTTATTCGTTGGAGACTTTATAACCCT	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11939_Mjap_Gib2022_COI	GLTB-007	1	BE11939_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	AATTTAATTGTGACTAGGCATGGTCTG	151.32538
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11941_Aamu_Bax2006_COI	GLTB-001	1	BE11941_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	GCACAACCGGGATCTTTACTTC	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11941_Pcan_Bot2011_IGS	GLTB-001	1	BE11941_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-001	IGS	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	CGTAATCCTCAGTACTGGCTA	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11941_Pper_Dia2013_COI	GLTB-001	1	BE11941_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	CTTAGTGGCATTAATTCGDAATCC	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11941_Sspa_Oph2007_COI	GLTB-001	1	BE11941_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	GAAGGCCTTCCGATGATC	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11941_Upin_Bot2011_COI	GLTB-001	1	BE11941_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	CTTTAATTACAGCGTTTTTATTGTTGT	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11941_Vgib_Wil2025_COI	GLTB-001	1	BE11941_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	CCCCTCTTTACAAGTTCTTTAGGGT	151.32441
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11942_Asen_Wil2025_COI	GLTB-002	1	BE11942_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	GTTGTTGTTACAACACATGCTCTTTTG	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11942_Cjap_Sim2018_COI	GLTB-002	1	BE11942_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11942_Esin_And2018_Cyb	GLTB-002	1	BE11942_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-002	Cytb	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	ACCCCTCCTCATATCCAACCA	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11942_Mgig_Bot2012_COI	GLTB-002	1	BE11942_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	TCTTATTCGTTGGAGACTTTATAACCCT	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11942_Mjap_Gib2022_COI	GLTB-002	1	BE11942_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	AATTTAATTGTGACTAGGCATGGTCTG	151.32348
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11943_Cmae_Bot2011_COI	GLTB-031	1	BE11943_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	ATGAACAGTCTATCCTCCTTTAG	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11943_Mros_Gib2022_COI	GLTB-031	1	BE11943_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11943_Msal_Bot2012_COI	GLTB-031	1	BE11943_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	GYTAGTTCCRATGATGTTAGCTG	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11943_Mstr_Wil2021_COI	GLTB-031	1	BE11943_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	GTAGTGGTAACTACTCATGCTTTA	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11943_Pvir_Dia2013_COI	GLTB-031	1	BE11943_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	CTTAGTGGCATTAATTCGDAATCC	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11943_Rhar_Sim2018_COI	GLTB-031	1	BE11943_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	CTGCTGCTATTGCTCATG	151.31176
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11945_Aamu_Bax2006_COI	GLTB-033	1	BE11945_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	GCACAACCGGGATCTTTACTTC	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11945_Pcan_Bot2011_IGS	GLTB-033	1	BE11945_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-033	IGS	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	CGTAATCCTCAGTACTGGCTA	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11945_Pper_Dia2013_COI	GLTB-033	1	BE11945_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	CTTAGTGGCATTAATTCGDAATCC	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11945_Sspa_Oph2007_COI	GLTB-033	1	BE11945_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	GAAGGCCTTCCGATGATC	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11945_Upin_Bot2011_COI	GLTB-033	1	BE11945_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	CTTTAATTACAGCGTTTTTATTGTTGT	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11945_Vgib_Wil2025_COI	GLTB-033	1	BE11945_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	CCCCTCTTTACAAGTTCTTTAGGGT	151.31518
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11946_Asen_Wil2025_COI	GLTB-034	1	BE11946_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	GTTGTTGTTACAACACATGCTCTTTTG	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11946_Cjap_Sim2018_COI	GLTB-034	1	BE11946_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11946_Esin_And2018_Cyb	GLTB-034	1	BE11946_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-034	Cytb	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	ACCCCTCCTCATATCCAACCA	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11946_Mgig_Bot2012_COI	GLTB-034	1	BE11946_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	TCTTATTCGTTGGAGACTTTATAACCCT	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11946_Mjap_Gib2022_COI	GLTB-034	1	BE11946_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	AATTTAATTGTGACTAGGCATGGTCTG	151.31117
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11947_Cmae_Bot2011_COI	GLTB-035	1	BE11947_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	ATGAACAGTCTATCCTCCTTTAG	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11947_Hsan_Wil2021_COI	GLTB-035	1	BE11947_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	CCTGGGCCGGTATAGTAGGT	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11947_Mros_Gib2022_COI	GLTB-035	1	BE11947_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11947_Msal_Bot2012_COI	GLTB-035	1	BE11947_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	GYTAGTTCCRATGATGTTAGCTG	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11947_Mstr_Wil2021_COI	GLTB-035	1	BE11947_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	GTAGTGGTAACTACTCATGCTTTA	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11947_Pvir_Dia2013_COI	GLTB-035	1	BE11947_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	CTTAGTGGCATTAATTCGDAATCC	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11947_Rhar_Sim2018_COI	GLTB-035	1	BE11947_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	CTGCTGCTATTGCTCATG	151.31271
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11952_Aamu_Bax2006_COI	GLTB-041	1	BE11952_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	GCACAACCGGGATCTTTACTTC	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11952_Pcan_Bot2011_IGS	GLTB-041	1	BE11952_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-041	IGS	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	CGTAATCCTCAGTACTGGCTA	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11952_Pper_Dia2013_COI	GLTB-041	1	BE11952_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	CTTAGTGGCATTAATTCGDAATCC	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11952_Sspa_Oph2007_COI	GLTB-041	1	BE11952_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	GAAGGCCTTCCGATGATC	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11952_Upin_Bot2011_COI	GLTB-041	1	BE11952_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	CTTTAATTACAGCGTTTTTATTGTTGT	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11952_Vgib_Wil2025_COI	GLTB-041	1	BE11952_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	CCCCTCTTTACAAGTTCTTTAGGGT	151.27678
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11953_Asen_Wil2025_COI	GLTB-042	1	BE11953_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	GTTGTTGTTACAACACATGCTCTTTTG	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11953_Cjap_Sim2018_COI	GLTB-042	1	BE11953_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11953_Esin_And2018_Cyb	GLTB-042	1	BE11953_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-042	Cytb	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	ACCCCTCCTCATATCCAACCA	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11953_Mgig_Bot2012_COI	GLTB-042	1	BE11953_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	TCTTATTCGTTGGAGACTTTATAACCCT	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11953_Mjap_Gib2022_COI	GLTB-042	1	BE11953_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	AATTTAATTGTGACTAGGCATGGTCTG	151.2668
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11954_Cmae_Bot2011_COI	GLTB-043	1	BE11954_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	ATGAACAGTCTATCCTCCTTTAG	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11954_Hsan_Wil2021_COI	GLTB-043	1	BE11954_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	CCTGGGCCGGTATAGTAGGT	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11954_Mros_Gib2022_COI	GLTB-043	1	BE11954_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11954_Msal_Bot2012_COI	GLTB-043	1	BE11954_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	GYTAGTTCCRATGATGTTAGCTG	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11954_Mstr_Wil2021_COI	GLTB-043	1	BE11954_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	GTAGTGGTAACTACTCATGCTTTA	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11954_Pvir_Dia2013_COI	GLTB-043	1	BE11954_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	CTTAGTGGCATTAATTCGDAATCC	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11954_Rhar_Sim2018_COI	GLTB-043	1	BE11954_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	CTGCTGCTATTGCTCATG	151.26832
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11956_Aamu_Bax2006_COI	GLTB-045	1	BE11956_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	GCACAACCGGGATCTTTACTTC	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11956_Pcan_Bot2011_IGS	GLTB-045	1	BE11956_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-045	IGS	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	CGTAATCCTCAGTACTGGCTA	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11956_Pper_Dia2013_COI	GLTB-045	1	BE11956_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	CTTAGTGGCATTAATTCGDAATCC	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11956_Sspa_Oph2007_COI	GLTB-045	1	BE11956_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	GAAGGCCTTCCGATGATC	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11956_Upin_Bot2011_COI	GLTB-045	1	BE11956_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	CTTTAATTACAGCGTTTTTATTGTTGT	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11956_Vgib_Wil2025_COI	GLTB-045	1	BE11956_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	CCCCTCTTTACAAGTTCTTTAGGGT	151.27238
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11957_Asen_Wil2025_COI	GLTB-046	1	BE11957_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	GTTGTTGTTACAACACATGCTCTTTTG	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11957_Cjap_Sim2018_COI	GLTB-046	1	BE11957_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11957_Esin_And2018_Cyb	GLTB-046	1	BE11957_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-046	Cytb	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	ACCCCTCCTCATATCCAACCA	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11957_Mgig_Bot2012_COI	GLTB-046	1	BE11957_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	TCTTATTCGTTGGAGACTTTATAACCCT	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11957_Mjap_Gib2022_COI	GLTB-046	1	BE11957_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	AATTTAATTGTGACTAGGCATGGTCTG	151.27382
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11958_Cmae_Bot2011_COI	GLTB-053	1	BE11958_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	ATGAACAGTCTATCCTCCTTTAG	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11958_Hsan_Wil2021_COI	GLTB-053	1	BE11958_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	CCTGGGCCGGTATAGTAGGT	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11958_Mros_Gib2022_COI	GLTB-053	1	BE11958_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11958_Msal_Bot2012_COI	GLTB-053	1	BE11958_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	GYTAGTTCCRATGATGTTAGCTG	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11958_Mstr_Wil2021_COI	GLTB-053	1	BE11958_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	GTAGTGGTAACTACTCATGCTTTA	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11958_Pvir_Dia2013_COI	GLTB-053	1	BE11958_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	CTTAGTGGCATTAATTCGDAATCC	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11958_Rhar_Sim2018_COI	GLTB-053	1	BE11958_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	CTGCTGCTATTGCTCATG	151.26226
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11960_Asen_Wil2025_COI	GLTB-048	1	BE11960_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	GTTGTTGTTACAACACATGCTCTTTTG	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11960_Cjap_Sim2018_COI	GLTB-048	1	BE11960_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11960_Esin_And2018_Cyb	GLTB-048	1	BE11960_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-048	Cytb	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	ACCCCTCCTCATATCCAACCA	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11960_Mgig_Bot2012_COI	GLTB-048	1	BE11960_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	TCTTATTCGTTGGAGACTTTATAACCCT	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11960_Mjap_Gib2022_COI	GLTB-048	1	BE11960_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	AATTTAATTGTGACTAGGCATGGTCTG	151.26536
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11961_Cmae_Bot2011_COI	GLTB-049	1	BE11961_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	ATGAACAGTCTATCCTCCTTTAG	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11961_Hsan_Wil2021_COI	GLTB-049	1	BE11961_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	CCTGGGCCGGTATAGTAGGT	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11961_Mros_Gib2022_COI	GLTB-049	1	BE11961_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11961_Msal_Bot2012_COI	GLTB-049	1	BE11961_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	GYTAGTTCCRATGATGTTAGCTG	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11961_Mstr_Wil2021_COI	GLTB-049	1	BE11961_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	GTAGTGGTAACTACTCATGCTTTA	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11961_Pvir_Dia2013_COI	GLTB-049	1	BE11961_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	CTTAGTGGCATTAATTCGDAATCC	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11961_Rhar_Sim2018_COI	GLTB-049	1	BE11961_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	CTGCTGCTATTGCTCATG	151.26732
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11963_Aamu_Bax2006_COI	GLTB-051	1	BE11963_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	GCACAACCGGGATCTTTACTTC	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11963_Pcan_Bot2011_IGS	GLTB-051	1	BE11963_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-051	IGS	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	CGTAATCCTCAGTACTGGCTA	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11963_Pper_Dia2013_COI	GLTB-051	1	BE11963_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	CTTAGTGGCATTAATTCGDAATCC	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11963_Sspa_Oph2007_COI	GLTB-051	1	BE11963_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	GAAGGCCTTCCGATGATC	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11963_Upin_Bot2011_COI	GLTB-051	1	BE11963_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	CTTTAATTACAGCGTTTTTATTGTTGT	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11963_Vgib_Wil2025_COI	GLTB-051	1	BE11963_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	CCCCTCTTTACAAGTTCTTTAGGGT	151.27092
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11964_Asen_Wil2025_COI	GLTB-052	1	BE11964_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	GTTGTTGTTACAACACATGCTCTTTTG	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11964_Cjap_Sim2018_COI	GLTB-052	1	BE11964_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11964_Esin_And2018_Cyb	GLTB-052	1	BE11964_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-052	Cytb	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	ACCCCTCCTCATATCCAACCA	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11964_Mgig_Bot2012_COI	GLTB-052	1	BE11964_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	TCTTATTCGTTGGAGACTTTATAACCCT	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11964_Mjap_Gib2022_COI	GLTB-052	1	BE11964_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	AATTTAATTGTGACTAGGCATGGTCTG	151.27254
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11965_Cmae_Bot2011_COI	GLTB-054	1	BE11965_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	ATGAACAGTCTATCCTCCTTTAG	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11965_Hsan_Wil2021_COI	GLTB-054	1	BE11965_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	CCTGGGCCGGTATAGTAGGT	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11965_Mros_Gib2022_COI	GLTB-054	1	BE11965_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11965_Msal_Bot2012_COI	GLTB-054	1	BE11965_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	GYTAGTTCCRATGATGTTAGCTG	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11965_Mstr_Wil2021_COI	GLTB-054	1	BE11965_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	GTAGTGGTAACTACTCATGCTTTA	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11965_Pvir_Dia2013_COI	GLTB-054	1	BE11965_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	CTTAGTGGCATTAATTCGDAATCC	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11965_Rhar_Sim2018_COI	GLTB-054	1	BE11965_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	CTGCTGCTATTGCTCATG	151.26444
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11967_Aamu_Bax2006_COI	GLTB-056	1	BE11967_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	GCACAACCGGGATCTTTACTTC	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11967_Pcan_Bot2011_IGS	GLTB-056	1	BE11967_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-056	IGS	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	CGTAATCCTCAGTACTGGCTA	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11967_Pper_Dia2013_COI	GLTB-056	1	BE11967_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	CTTAGTGGCATTAATTCGDAATCC	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11967_Sspa_Oph2007_COI	GLTB-056	1	BE11967_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	GAAGGCCTTCCGATGATC	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11967_Upin_Bot2011_COI	GLTB-056	1	BE11967_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	CTTTAATTACAGCGTTTTTATTGTTGT	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11967_Vgib_Wil2025_COI	GLTB-056	1	BE11967_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	CCCCTCTTTACAAGTTCTTTAGGGT	151.26783
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11968_Asen_Wil2025_COI	GLTB-057	1	BE11968_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	GTTGTTGTTACAACACATGCTCTTTTG	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11968_Cjap_Sim2018_COI	GLTB-057	1	BE11968_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11968_Esin_And2018_Cyb	GLTB-057	1	BE11968_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-057	Cytb	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	ACCCCTCCTCATATCCAACCA	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11968_Mgig_Bot2012_COI	GLTB-057	1	BE11968_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	TCTTATTCGTTGGAGACTTTATAACCCT	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11968_Mjap_Gib2022_COI	GLTB-057	1	BE11968_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	AATTTAATTGTGACTAGGCATGGTCTG	151.25752
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11969_Cmae_Bot2011_COI	GLTB-058	1	BE11969_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	ATGAACAGTCTATCCTCCTTTAG	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11969_Hsan_Wil2021_COI	GLTB-058	1	BE11969_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	CCTGGGCCGGTATAGTAGGT	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11969_Mros_Gib2022_COI	GLTB-058	1	BE11969_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11969_Msal_Bot2012_COI	GLTB-058	1	BE11969_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	GYTAGTTCCRATGATGTTAGCTG	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11969_Mstr_Wil2021_COI	GLTB-058	1	BE11969_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	GTAGTGGTAACTACTCATGCTTTA	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11969_Pvir_Dia2013_COI	GLTB-058	1	BE11969_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	CTTAGTGGCATTAATTCGDAATCC	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11969_Rhar_Sim2018_COI	GLTB-058	1	BE11969_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	CTGCTGCTATTGCTCATG	151.25955
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11970_Aamu_Bax2006_COI	GLTB-059	1	BE11970_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	GCACAACCGGGATCTTTACTTC	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11970_Pcan_Bot2011_IGS	GLTB-059	1	BE11970_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-059	IGS	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	CGTAATCCTCAGTACTGGCTA	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11970_Pper_Dia2013_COI	GLTB-059	1	BE11970_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	CTTAGTGGCATTAATTCGDAATCC	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11970_Sspa_Oph2007_COI	GLTB-059	1	BE11970_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	GAAGGCCTTCCGATGATC	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11970_Upin_Bot2011_COI	GLTB-059	1	BE11970_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	CTTTAATTACAGCGTTTTTATTGTTGT	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11970_Vgib_Wil2025_COI	GLTB-059	1	BE11970_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	CCCCTCTTTACAAGTTCTTTAGGGT	151.26152
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11971_Asen_Wil2025_COI	GLTB-060	1	BE11971_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	GTTGTTGTTACAACACATGCTCTTTTG	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11971_Cjap_Sim2018_COI	GLTB-060	1	BE11971_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11971_Esin_And2018_Cyb	GLTB-060	1	BE11971_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-060	Cytb	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	ACCCCTCCTCATATCCAACCA	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11971_Mgig_Bot2012_COI	GLTB-060	1	BE11971_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	TCTTATTCGTTGGAGACTTTATAACCCT	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11971_Mjap_Gib2022_COI	GLTB-060	1	BE11971_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	AATTTAATTGTGACTAGGCATGGTCTG	151.26334
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11972_Cmae_Bot2011_COI	GLTB-061	1	BE11972_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	ATGAACAGTCTATCCTCCTTTAG	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11972_Hsan_Wil2021_COI	GLTB-061	1	BE11972_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	CCTGGGCCGGTATAGTAGGT	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11972_Mros_Gib2022_COI	GLTB-061	1	BE11972_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11972_Msal_Bot2012_COI	GLTB-061	1	BE11972_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	GYTAGTTCCRATGATGTTAGCTG	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11972_Mstr_Wil2021_COI	GLTB-061	1	BE11972_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	GTAGTGGTAACTACTCATGCTTTA	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11972_Pvir_Dia2013_COI	GLTB-061	1	BE11972_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	CTTAGTGGCATTAATTCGDAATCC	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11972_Rhar_Sim2018_COI	GLTB-061	1	BE11972_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	CTGCTGCTATTGCTCATG	151.26491
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11974_Aamu_Bax2006_COI	GLTB-063	1	BE11974_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	GCACAACCGGGATCTTTACTTC	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11974_Pcan_Bot2011_IGS	GLTB-063	1	BE11974_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-063	IGS	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	CGTAATCCTCAGTACTGGCTA	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11974_Pper_Dia2013_COI	GLTB-063	1	BE11974_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	CTTAGTGGCATTAATTCGDAATCC	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11974_Sspa_Oph2007_COI	GLTB-063	1	BE11974_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	GAAGGCCTTCCGATGATC	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11974_Upin_Bot2011_COI	GLTB-063	1	BE11974_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	CTTTAATTACAGCGTTTTTATTGTTGT	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11974_Vgib_Wil2025_COI	GLTB-063	1	BE11974_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	CCCCTCTTTACAAGTTCTTTAGGGT	151.25482
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11975_Asen_Wil2025_COI	GLTB-064	1	BE11975_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	GTTGTTGTTACAACACATGCTCTTTTG	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11975_Cjap_Sim2018_COI	GLTB-064	1	BE11975_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11975_Esin_And2018_Cyb	GLTB-064	1	BE11975_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-064	Cytb	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	ACCCCTCCTCATATCCAACCA	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11975_Mgig_Bot2012_COI	GLTB-064	1	BE11975_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	TCTTATTCGTTGGAGACTTTATAACCCT	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11975_Mjap_Gib2022_COI	GLTB-064	1	BE11975_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	AATTTAATTGTGACTAGGCATGGTCTG	151.25657
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11976_Cmae_Bot2011_COI	GLTB-065	1	BE11976_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	ATGAACAGTCTATCCTCCTTTAG	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11976_Hsan_Wil2021_COI	GLTB-065	1	BE11976_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	CCTGGGCCGGTATAGTAGGT	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11976_Mros_Gib2022_COI	GLTB-065	1	BE11976_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11976_Msal_Bot2012_COI	GLTB-065	1	BE11976_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	GYTAGTTCCRATGATGTTAGCTG	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11976_Mstr_Wil2021_COI	GLTB-065	1	BE11976_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	GTAGTGGTAACTACTCATGCTTTA	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11976_Pvir_Dia2013_COI	GLTB-065	1	BE11976_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	CTTAGTGGCATTAATTCGDAATCC	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11976_Rhar_Sim2018_COI	GLTB-065	1	BE11976_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	CTGCTGCTATTGCTCATG	151.25842
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11978_Aamu_Bax2006_COI	GLTB-067	1	BE11978_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	GCACAACCGGGATCTTTACTTC	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11978_Pcan_Bot2011_IGS	GLTB-067	1	BE11978_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-067	IGS	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	CGTAATCCTCAGTACTGGCTA	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11978_Pper_Dia2013_COI	GLTB-067	1	BE11978_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	CTTAGTGGCATTAATTCGDAATCC	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11978_Sspa_Oph2007_COI	GLTB-067	1	BE11978_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	GAAGGCCTTCCGATGATC	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11978_Upin_Bot2011_COI	GLTB-067	1	BE11978_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	CTTTAATTACAGCGTTTTTATTGTTGT	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11978_Vgib_Wil2025_COI	GLTB-067	1	BE11978_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	CCCCTCTTTACAAGTTCTTTAGGGT	151.26178
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11979_Asen_Wil2025_COI	GLTB-068	1	BE11979_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	GTTGTTGTTACAACACATGCTCTTTTG	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11979_Cjap_Sim2018_COI	GLTB-068	1	BE11979_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11979_Esin_And2018_Cyb	GLTB-068	1	BE11979_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-068	Cytb	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	ACCCCTCCTCATATCCAACCA	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11979_Mgig_Bot2012_COI	GLTB-068	1	BE11979_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	TCTTATTCGTTGGAGACTTTATAACCCT	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11979_Mjap_Gib2022_COI	GLTB-068	1	BE11979_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	AATTTAATTGTGACTAGGCATGGTCTG	151.25338
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11981_Aamu_Bax2006_COI	GLTB-070	1	BE11981_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	GCACAACCGGGATCTTTACTTC	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11981_Pcan_Bot2011_IGS	GLTB-070	1	BE11981_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-070	IGS	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	CGTAATCCTCAGTACTGGCTA	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11981_Pper_Dia2013_COI	GLTB-070	1	BE11981_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	CTTAGTGGCATTAATTCGDAATCC	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11981_Sspa_Oph2007_COI	GLTB-070	1	BE11981_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	GAAGGCCTTCCGATGATC	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11981_Upin_Bot2011_COI	GLTB-070	1	BE11981_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	CTTTAATTACAGCGTTTTTATTGTTGT	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11981_Vgib_Wil2025_COI	GLTB-070	1	BE11981_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	CCCCTCTTTACAAGTTCTTTAGGGT	151.2573
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11982_Asen_Wil2025_COI	GLTB-036	1	BE11982_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	GTTGTTGTTACAACACATGCTCTTTTG	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11982_Cjap_Sim2018_COI	GLTB-036	1	BE11982_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11982_Esin_And2018_Cyb	GLTB-036	1	BE11982_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-036	Cytb	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	ACCCCTCCTCATATCCAACCA	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11982_Mgig_Bot2012_COI	GLTB-036	1	BE11982_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	TCTTATTCGTTGGAGACTTTATAACCCT	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11982_Mjap_Gib2022_COI	GLTB-036	1	BE11982_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	AATTTAATTGTGACTAGGCATGGTCTG	151.27472
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11983_Cmae_Bot2011_COI	GLTB-037	1	BE11983_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	ATGAACAGTCTATCCTCCTTTAG	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11983_Hsan_Wil2021_COI	GLTB-037	1	BE11983_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	CCTGGGCCGGTATAGTAGGT	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11983_Mros_Gib2022_COI	GLTB-037	1	BE11983_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11983_Msal_Bot2012_COI	GLTB-037	1	BE11983_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	GYTAGTTCCRATGATGTTAGCTG	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11983_Mstr_Wil2021_COI	GLTB-037	1	BE11983_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	GTAGTGGTAACTACTCATGCTTTA	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11983_Pvir_Dia2013_COI	GLTB-037	1	BE11983_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	CTTAGTGGCATTAATTCGDAATCC	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11983_Rhar_Sim2018_COI	GLTB-037	1	BE11983_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	CTGCTGCTATTGCTCATG	151.27454
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11985_Aamu_Bax2006_COI	GLTB-039	1	BE11985_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	GCACAACCGGGATCTTTACTTC	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11985_Pcan_Bot2011_IGS	GLTB-039	1	BE11985_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-039	IGS	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	CGTAATCCTCAGTACTGGCTA	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11985_Pper_Dia2013_COI	GLTB-039	1	BE11985_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	CTTAGTGGCATTAATTCGDAATCC	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11985_Sspa_Oph2007_COI	GLTB-039	1	BE11985_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	GAAGGCCTTCCGATGATC	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11985_Upin_Bot2011_COI	GLTB-039	1	BE11985_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	CTTTAATTACAGCGTTTTTATTGTTGT	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11985_Vgib_Wil2025_COI	GLTB-039	1	BE11985_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	CCCCTCTTTACAAGTTCTTTAGGGT	151.27637
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11986_Asen_Wil2025_COI	GLTB-040	1	BE11986_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	GTTGTTGTTACAACACATGCTCTTTTG	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11986_Cjap_Sim2018_COI	GLTB-040	1	BE11986_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11986_Esin_And2018_Cyb	GLTB-040	1	BE11986_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-040	Cytb	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	ACCCCTCCTCATATCCAACCA	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11986_Mgig_Bot2012_COI	GLTB-040	1	BE11986_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	TCTTATTCGTTGGAGACTTTATAACCCT	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11986_Mjap_Gib2022_COI	GLTB-040	1	BE11986_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	AATTTAATTGTGACTAGGCATGGTCTG	151.27501
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11992_Aamu_Bax2006_COI	GLTB-072	1	BE11992_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	GCACAACCGGGATCTTTACTTC	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11992_Pcan_Bot2011_IGS	GLTB-072	1	BE11992_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-072	IGS	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	CGTAATCCTCAGTACTGGCTA	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11992_Pper_Dia2013_COI	GLTB-072	1	BE11992_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	CTTAGTGGCATTAATTCGDAATCC	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11992_Sspa_Oph2007_COI	GLTB-072	1	BE11992_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	GAAGGCCTTCCGATGATC	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11992_Upin_Bot2011_COI	GLTB-072	1	BE11992_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	CTTTAATTACAGCGTTTTTATTGTTGT	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11992_Vgib_Wil2025_COI	GLTB-072	1	BE11992_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	CCCCTCTTTACAAGTTCTTTAGGGT	151.1841
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11993_Asen_Wil2025_COI	GLTB-073	1	BE11993_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	GTTGTTGTTACAACACATGCTCTTTTG	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11993_Cjap_Sim2018_COI	GLTB-073	1	BE11993_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11993_Esin_And2018_Cyb	GLTB-073	1	BE11993_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-073	Cytb	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	ACCCCTCCTCATATCCAACCA	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11993_Mgig_Bot2012_COI	GLTB-073	1	BE11993_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	TCTTATTCGTTGGAGACTTTATAACCCT	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11993_Mjap_Gib2022_COI	GLTB-073	1	BE11993_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	AATTTAATTGTGACTAGGCATGGTCTG	151.18557
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11994_Cmae_Bot2011_COI	GLTB-074	1	BE11994_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	ATGAACAGTCTATCCTCCTTTAG	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11994_Hsan_Wil2021_COI	GLTB-074	1	BE11994_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	CCTGGGCCGGTATAGTAGGT	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11994_Mros_Gib2022_COI	GLTB-074	1	BE11994_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11994_Msal_Bot2012_COI	GLTB-074	1	BE11994_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	GYTAGTTCCRATGATGTTAGCTG	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11994_Mstr_Wil2021_COI	GLTB-074	1	BE11994_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	GTAGTGGTAACTACTCATGCTTTA	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11994_Pvir_Dia2013_COI	GLTB-074	1	BE11994_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	CTTAGTGGCATTAATTCGDAATCC	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11994_Rhar_Sim2018_COI	GLTB-074	1	BE11994_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	CTGCTGCTATTGCTCATG	151.18773
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11996_Aamu_Bax2006_COI	GLTB-076	1	BE11996_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	GCACAACCGGGATCTTTACTTC	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11996_Pcan_Bot2011_IGS	GLTB-076	1	BE11996_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-076	IGS	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	CGTAATCCTCAGTACTGGCTA	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11996_Pper_Dia2013_COI	GLTB-076	1	BE11996_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	CTTAGTGGCATTAATTCGDAATCC	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11996_Sspa_Oph2007_COI	GLTB-076	1	BE11996_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	GAAGGCCTTCCGATGATC	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11996_Upin_Bot2011_COI	GLTB-076	1	BE11996_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	CTTTAATTACAGCGTTTTTATTGTTGT	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11996_Vgib_Wil2025_COI	GLTB-076	1	BE11996_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	CCCCTCTTTACAAGTTCTTTAGGGT	151.18546
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11997_Asen_Wil2025_COI	GLTB-077	1	BE11997_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	GTTGTTGTTACAACACATGCTCTTTTG	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11997_Cjap_Sim2018_COI	GLTB-077	1	BE11997_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11997_Esin_And2018_Cyb	GLTB-077	1	BE11997_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-077	Cytb	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	ACCCCTCCTCATATCCAACCA	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11997_Mgig_Bot2012_COI	GLTB-077	1	BE11997_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	TCTTATTCGTTGGAGACTTTATAACCCT	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11997_Mjap_Gib2022_COI	GLTB-077	1	BE11997_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	AATTTAATTGTGACTAGGCATGGTCTG	151.18751
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11998_Cmae_Bot2011_COI	GLTB-078	1	BE11998_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	ATGAACAGTCTATCCTCCTTTAG	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11998_Hsan_Wil2021_COI	GLTB-078	1	BE11998_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	CCTGGGCCGGTATAGTAGGT	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11998_Mros_Gib2022_COI	GLTB-078	1	BE11998_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11998_Msal_Bot2012_COI	GLTB-078	1	BE11998_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	GYTAGTTCCRATGATGTTAGCTG	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11998_Mstr_Wil2021_COI	GLTB-078	1	BE11998_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	GTAGTGGTAACTACTCATGCTTTA	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11998_Pvir_Dia2013_COI	GLTB-078	1	BE11998_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	CTTAGTGGCATTAATTCGDAATCC	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11998_Rhar_Sim2018_COI	GLTB-078	1	BE11998_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	CTGCTGCTATTGCTCATG	151.18853
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12000_Asen_Wil2025_COI	GLTB-080	1	BE12000_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	GTTGTTGTTACAACACATGCTCTTTTG	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12000_Cjap_Sim2018_COI	GLTB-080	1	BE12000_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12000_Esin_And2018_Cyb	GLTB-080	1	BE12000_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-080	Cytb	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	ACCCCTCCTCATATCCAACCA	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12000_Mgig_Bot2012_COI	GLTB-080	1	BE12000_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	TCTTATTCGTTGGAGACTTTATAACCCT	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12000_Mjap_Gib2022_COI	GLTB-080	1	BE12000_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	AATTTAATTGTGACTAGGCATGGTCTG	151.18576
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12001_Cmae_Bot2011_COI	GLTB-081	1	BE12001_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	ATGAACAGTCTATCCTCCTTTAG	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12001_Hsan_Wil2021_COI	GLTB-081	1	BE12001_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	CCTGGGCCGGTATAGTAGGT	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12001_Mros_Gib2022_COI	GLTB-081	1	BE12001_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12001_Msal_Bot2012_COI	GLTB-081	1	BE12001_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	GYTAGTTCCRATGATGTTAGCTG	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12001_Mstr_Wil2021_COI	GLTB-081	1	BE12001_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	GTAGTGGTAACTACTCATGCTTTA	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12001_Pvir_Dia2013_COI	GLTB-081	1	BE12001_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	CTTAGTGGCATTAATTCGDAATCC	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12001_Rhar_Sim2018_COI	GLTB-081	1	BE12001_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	CTGCTGCTATTGCTCATG	151.1876
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12003_Aamu_Bax2006_COI	GLTB-083	1	BE12003_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	GCACAACCGGGATCTTTACTTC	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12003_Pcan_Bot2011_IGS	GLTB-083	1	BE12003_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-083	IGS	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	CGTAATCCTCAGTACTGGCTA	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12003_Pper_Dia2013_COI	GLTB-083	1	BE12003_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	CTTAGTGGCATTAATTCGDAATCC	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12003_Sspa_Oph2007_COI	GLTB-083	1	BE12003_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	GAAGGCCTTCCGATGATC	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12003_Upin_Bot2011_COI	GLTB-083	1	BE12003_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	CTTTAATTACAGCGTTTTTATTGTTGT	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12003_Vgib_Wil2025_COI	GLTB-083	1	BE12003_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	CCCCTCTTTACAAGTTCTTTAGGGT	151.18459
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12004_Asen_Wil2025_COI	GLTB-084	1	BE12004_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	GTTGTTGTTACAACACATGCTCTTTTG	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12004_Cjap_Sim2018_COI	GLTB-084	1	BE12004_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12004_Esin_And2018_Cyb	GLTB-084	1	BE12004_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-084	Cytb	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	ACCCCTCCTCATATCCAACCA	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12004_Mgig_Bot2012_COI	GLTB-084	1	BE12004_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	TCTTATTCGTTGGAGACTTTATAACCCT	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12004_Mjap_Gib2022_COI	GLTB-084	1	BE12004_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	AATTTAATTGTGACTAGGCATGGTCTG	151.18668
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12005_Cmae_Bot2011_COI	GLTB-085	1	BE12005_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	ATGAACAGTCTATCCTCCTTTAG	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12005_Hsan_Wil2021_COI	GLTB-085	1	BE12005_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	CCTGGGCCGGTATAGTAGGT	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12005_Mros_Gib2022_COI	GLTB-085	1	BE12005_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12005_Msal_Bot2012_COI	GLTB-085	1	BE12005_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	GYTAGTTCCRATGATGTTAGCTG	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12005_Mstr_Wil2021_COI	GLTB-085	1	BE12005_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	GTAGTGGTAACTACTCATGCTTTA	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12005_Pvir_Dia2013_COI	GLTB-085	1	BE12005_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	CTTAGTGGCATTAATTCGDAATCC	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12005_Rhar_Sim2018_COI	GLTB-085	1	BE12005_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	CTGCTGCTATTGCTCATG	151.18209
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12007_Aamu_Bax2006_COI	GLTB-087	1	BE12007_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	GCACAACCGGGATCTTTACTTC	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12007_Pcan_Bot2011_IGS	GLTB-087	1	BE12007_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-087	IGS	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	CGTAATCCTCAGTACTGGCTA	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12007_Pper_Dia2013_COI	GLTB-087	1	BE12007_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	CTTAGTGGCATTAATTCGDAATCC	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12007_Sspa_Oph2007_COI	GLTB-087	1	BE12007_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	GAAGGCCTTCCGATGATC	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12007_Upin_Bot2011_COI	GLTB-087	1	BE12007_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	CTTTAATTACAGCGTTTTTATTGTTGT	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12007_Vgib_Wil2025_COI	GLTB-087	1	BE12007_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	CCCCTCTTTACAAGTTCTTTAGGGT	151.18521
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12008_Asen_Wil2025_COI	GLTB-088	1	BE12008_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	GTTGTTGTTACAACACATGCTCTTTTG	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12008_Cjap_Sim2018_COI	GLTB-088	1	BE12008_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12008_Esin_And2018_Cyb	GLTB-088	1	BE12008_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-088	Cytb	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	ACCCCTCCTCATATCCAACCA	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12008_Mgig_Bot2012_COI	GLTB-088	1	BE12008_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	TCTTATTCGTTGGAGACTTTATAACCCT	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12008_Mjap_Gib2022_COI	GLTB-088	1	BE12008_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	AATTTAATTGTGACTAGGCATGGTCTG	151.18112
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12009_Cmae_Bot2011_COI	GLTB-089	1	BE12009_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	ATGAACAGTCTATCCTCCTTTAG	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12009_Hsan_Wil2021_COI	GLTB-089	1	BE12009_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	CCTGGGCCGGTATAGTAGGT	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12009_Mros_Gib2022_COI	GLTB-089	1	BE12009_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12009_Msal_Bot2012_COI	GLTB-089	1	BE12009_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	GYTAGTTCCRATGATGTTAGCTG	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12009_Mstr_Wil2021_COI	GLTB-089	1	BE12009_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	GTAGTGGTAACTACTCATGCTTTA	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12009_Pvir_Dia2013_COI	GLTB-089	1	BE12009_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	CTTAGTGGCATTAATTCGDAATCC	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12009_Rhar_Sim2018_COI	GLTB-089	1	BE12009_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	CTGCTGCTATTGCTCATG	151.18263
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12010_Aamu_Bax2006_COI	GLTB-090	1	BE12010_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	GCACAACCGGGATCTTTACTTC	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12010_Pcan_Bot2011_IGS	GLTB-090	1	BE12010_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-090	IGS	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	CGTAATCCTCAGTACTGGCTA	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12010_Pper_Dia2013_COI	GLTB-090	1	BE12010_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	CTTAGTGGCATTAATTCGDAATCC	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12010_Sspa_Oph2007_COI	GLTB-090	1	BE12010_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	GAAGGCCTTCCGATGATC	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12010_Upin_Bot2011_COI	GLTB-090	1	BE12010_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	CTTTAATTACAGCGTTTTTATTGTTGT	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12010_Vgib_Wil2025_COI	GLTB-090	1	BE12010_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	CCCCTCTTTACAAGTTCTTTAGGGT	151.1843
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12011_Asen_Wil2025_COI	GLTB-091	1	BE12011_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	GTTGTTGTTACAACACATGCTCTTTTG	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12011_Cjap_Sim2018_COI	GLTB-091	1	BE12011_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12011_Esin_And2018_Cyb	GLTB-091	1	BE12011_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-091	Cytb	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	ACCCCTCCTCATATCCAACCA	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12011_Mgig_Bot2012_COI	GLTB-091	1	BE12011_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	TCTTATTCGTTGGAGACTTTATAACCCT	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12011_Mjap_Gib2022_COI	GLTB-091	1	BE12011_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	AATTTAATTGTGACTAGGCATGGTCTG	151.17847
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12012_Cmae_Bot2011_COI	GLTB-092	1	BE12012_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	ATGAACAGTCTATCCTCCTTTAG	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12012_Hsan_Wil2021_COI	GLTB-092	1	BE12012_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	CCTGGGCCGGTATAGTAGGT	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12012_Mros_Gib2022_COI	GLTB-092	1	BE12012_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12012_Msal_Bot2012_COI	GLTB-092	1	BE12012_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	GYTAGTTCCRATGATGTTAGCTG	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12012_Mstr_Wil2021_COI	GLTB-092	1	BE12012_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	GTAGTGGTAACTACTCATGCTTTA	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12012_Pvir_Dia2013_COI	GLTB-092	1	BE12012_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	CTTAGTGGCATTAATTCGDAATCC	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12012_Rhar_Sim2018_COI	GLTB-092	1	BE12012_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	CTGCTGCTATTGCTCATG	151.17995
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12014_Aamu_Bax2006_COI	GLTB-094	1	BE12014_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	GCACAACCGGGATCTTTACTTC	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12014_Pcan_Bot2011_IGS	GLTB-094	1	BE12014_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-094	IGS	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	CGTAATCCTCAGTACTGGCTA	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12014_Pper_Dia2013_COI	GLTB-094	1	BE12014_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	CTTAGTGGCATTAATTCGDAATCC	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12014_Sspa_Oph2007_COI	GLTB-094	1	BE12014_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	GAAGGCCTTCCGATGATC	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12014_Upin_Bot2011_COI	GLTB-094	1	BE12014_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	CTTTAATTACAGCGTTTTTATTGTTGT	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12014_Vgib_Wil2025_COI	GLTB-094	1	BE12014_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	CCCCTCTTTACAAGTTCTTTAGGGT	151.17722
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12015_Asen_Wil2025_COI	GLTB-095	1	BE12015_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	GTTGTTGTTACAACACATGCTCTTTTG	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12015_Cjap_Sim2018_COI	GLTB-095	1	BE12015_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12015_Esin_And2018_Cyb	GLTB-095	1	BE12015_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-095	Cytb	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	ACCCCTCCTCATATCCAACCA	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12015_Mgig_Bot2012_COI	GLTB-095	1	BE12015_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	TCTTATTCGTTGGAGACTTTATAACCCT	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12015_Mjap_Gib2022_COI	GLTB-095	1	BE12015_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	AATTTAATTGTGACTAGGCATGGTCTG	151.17895
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12016_Cmae_Bot2011_COI	GLTB-096	1	BE12016_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	ATGAACAGTCTATCCTCCTTTAG	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12016_Hsan_Wil2021_COI	GLTB-096	1	BE12016_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	CCTGGGCCGGTATAGTAGGT	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12016_Mros_Gib2022_COI	GLTB-096	1	BE12016_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12016_Msal_Bot2012_COI	GLTB-096	1	BE12016_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	GYTAGTTCCRATGATGTTAGCTG	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12016_Mstr_Wil2021_COI	GLTB-096	1	BE12016_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	GTAGTGGTAACTACTCATGCTTTA	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12016_Pvir_Dia2013_COI	GLTB-096	1	BE12016_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	CTTAGTGGCATTAATTCGDAATCC	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12016_Rhar_Sim2018_COI	GLTB-096	1	BE12016_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	CTGCTGCTATTGCTCATG	151.18077
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12018_Aamu_Bax2006_COI	GLTB-098	1	BE12018_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	GCACAACCGGGATCTTTACTTC	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12018_Pcan_Bot2011_IGS	GLTB-098	1	BE12018_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-098	IGS	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	CGTAATCCTCAGTACTGGCTA	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12018_Pper_Dia2013_COI	GLTB-098	1	BE12018_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	CTTAGTGGCATTAATTCGDAATCC	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12018_Sspa_Oph2007_COI	GLTB-098	1	BE12018_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	GAAGGCCTTCCGATGATC	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12018_Upin_Bot2011_COI	GLTB-098	1	BE12018_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	CTTTAATTACAGCGTTTTTATTGTTGT	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12018_Vgib_Wil2025_COI	GLTB-098	1	BE12018_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	CCCCTCTTTACAAGTTCTTTAGGGT	151.17811
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12019_Asen_Wil2025_COI	GLTB-099	1	BE12019_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	GTTGTTGTTACAACACATGCTCTTTTG	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12019_Cjap_Sim2018_COI	GLTB-099	1	BE12019_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12019_Esin_And2018_Cyb	GLTB-099	1	BE12019_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-099	Cytb	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	ACCCCTCCTCATATCCAACCA	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12019_Mgig_Bot2012_COI	GLTB-099	1	BE12019_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	TCTTATTCGTTGGAGACTTTATAACCCT	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12019_Mjap_Gib2022_COI	GLTB-099	1	BE12019_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	AATTTAATTGTGACTAGGCATGGTCTG	151.17342
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12021_Aamu_Bax2006_COI	GLTB-101	1	BE12021_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	GCACAACCGGGATCTTTACTTC	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12021_Pcan_Bot2011_IGS	GLTB-101	1	BE12021_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-101	IGS	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	CGTAATCCTCAGTACTGGCTA	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12021_Pper_Dia2013_COI	GLTB-101	1	BE12021_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	CTTAGTGGCATTAATTCGDAATCC	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12021_Sspa_Oph2007_COI	GLTB-101	1	BE12021_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	GAAGGCCTTCCGATGATC	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12021_Upin_Bot2011_COI	GLTB-101	1	BE12021_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	CTTTAATTACAGCGTTTTTATTGTTGT	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12021_Vgib_Wil2025_COI	GLTB-101	1	BE12021_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	CCCCTCTTTACAAGTTCTTTAGGGT	151.17671
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12022_Asen_Wil2025_COI	GLTB-102	1	BE12022_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	GTTGTTGTTACAACACATGCTCTTTTG	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12022_Cjap_Sim2018_COI	GLTB-102	1	BE12022_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12022_Esin_And2018_Cyb	GLTB-102	1	BE12022_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-102	Cytb	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	ACCCCTCCTCATATCCAACCA	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12022_Mgig_Bot2012_COI	GLTB-102	1	BE12022_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	TCTTATTCGTTGGAGACTTTATAACCCT	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12022_Mjap_Gib2022_COI	GLTB-102	1	BE12022_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	AATTTAATTGTGACTAGGCATGGTCTG	151.17257
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12023_Cmae_Bot2011_COI	GLTB-103	1	BE12023_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	ATGAACAGTCTATCCTCCTTTAG	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12023_Hsan_Wil2021_COI	GLTB-103	1	BE12023_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	CCTGGGCCGGTATAGTAGGT	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12023_Mros_Gib2022_COI	GLTB-103	1	BE12023_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12023_Msal_Bot2012_COI	GLTB-103	1	BE12023_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	GYTAGTTCCRATGATGTTAGCTG	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12023_Mstr_Wil2021_COI	GLTB-103	1	BE12023_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	GTAGTGGTAACTACTCATGCTTTA	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12023_Pvir_Dia2013_COI	GLTB-103	1	BE12023_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	CTTAGTGGCATTAATTCGDAATCC	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12023_Rhar_Sim2018_COI	GLTB-103	1	BE12023_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	CTGCTGCTATTGCTCATG	151.17423
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12025_Aamu_Bax2006_COI	GLTB-105	1	BE12025_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	GCACAACCGGGATCTTTACTTC	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12025_Pcan_Bot2011_IGS	GLTB-105	1	BE12025_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-105	IGS	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	CGTAATCCTCAGTACTGGCTA	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12025_Pper_Dia2013_COI	GLTB-105	1	BE12025_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	CTTAGTGGCATTAATTCGDAATCC	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12025_Sspa_Oph2007_COI	GLTB-105	1	BE12025_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	GAAGGCCTTCCGATGATC	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12025_Upin_Bot2011_COI	GLTB-105	1	BE12025_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	CTTTAATTACAGCGTTTTTATTGTTGT	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12025_Vgib_Wil2025_COI	GLTB-105	1	BE12025_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	CCCCTCTTTACAAGTTCTTTAGGGT	151.17319
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32692_Cmae_Bot2011_COI	CNSB-001	1	BE32692_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	ATGAACAGTCTATCCTCCTTTAG	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32692_Hsan_Wil2021_COI	CNSB-001	1	BE32692_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	CCTGGGCCGGTATAGTAGGT	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32692_Mros_Gib2022_COI	CNSB-001	1	BE32692_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32692_Msal_Bot2012_COI	CNSB-001	1	BE32692_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	GYTAGTTCCRATGATGTTAGCTG	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32692_Mstr_Wil2021_COI	CNSB-001	1	BE32692_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	GTAGTGGTAACTACTCATGCTTTA	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32692_Pvir_Dia2013_COI	CNSB-001	1	BE32692_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	CTTAGTGGCATTAATTCGDAATCC	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32692_Rhar_Sim2018_COI	CNSB-001	1	BE32692_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	CTGCTGCTATTGCTCATG	145.77391
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32694_Aamu_Bax2006_COI	CNSB-003	1	BE32694_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	GCACAACCGGGATCTTTACTTC	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32694_Pcan_Bot2011_IGS	CNSB-003	1	BE32694_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-003	IGS	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	CGTAATCCTCAGTACTGGCTA	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32694_Pper_Dia2013_COI	CNSB-003	1	BE32694_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	CTTAGTGGCATTAATTCGDAATCC	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32694_Sspa_Oph2007_COI	CNSB-003	1	BE32694_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	GAAGGCCTTCCGATGATC	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32694_Upin_Bot2011_COI	CNSB-003	1	BE32694_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	CTTTAATTACAGCGTTTTTATTGTTGT	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32694_Vgib_Wil2025_COI	CNSB-003	1	BE32694_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	CCCCTCTTTACAAGTTCTTTAGGGT	145.7747
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32695_Asen_Wil2025_COI	CNSB-004	1	BE32695_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	GTTGTTGTTACAACACATGCTCTTTTG	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32695_Cjap_Sim2018_COI	CNSB-004	1	BE32695_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32695_Esin_And2018_Cyb	CNSB-004	1	BE32695_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-004	Cytb	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	ACCCCTCCTCATATCCAACCA	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32695_Mgig_Bot2012_COI	CNSB-004	1	BE32695_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	TCTTATTCGTTGGAGACTTTATAACCCT	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32695_Mjap_Gib2022_COI	CNSB-004	1	BE32695_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	AATTTAATTGTGACTAGGCATGGTCTG	145.7763
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32696_Cmae_Bot2011_COI	CNSB-005	1	BE32696_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	ATGAACAGTCTATCCTCCTTTAG	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32696_Hsan_Wil2021_COI	CNSB-005	1	BE32696_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	CCTGGGCCGGTATAGTAGGT	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32696_Mros_Gib2022_COI	CNSB-005	1	BE32696_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32696_Msal_Bot2012_COI	CNSB-005	1	BE32696_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	GYTAGTTCCRATGATGTTAGCTG	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32696_Mstr_Wil2021_COI	CNSB-005	1	BE32696_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	GTAGTGGTAACTACTCATGCTTTA	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32696_Pvir_Dia2013_COI	CNSB-005	1	BE32696_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	CTTAGTGGCATTAATTCGDAATCC	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32696_Rhar_Sim2018_COI	CNSB-005	1	BE32696_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	CTGCTGCTATTGCTCATG	145.77936
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32698_Aamu_Bax2006_COI	CNSB-007	1	BE32698_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	GCACAACCGGGATCTTTACTTC	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32698_Pcan_Bot2011_IGS	CNSB-007	1	BE32698_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-007	IGS	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	CGTAATCCTCAGTACTGGCTA	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32698_Pper_Dia2013_COI	CNSB-007	1	BE32698_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	CTTAGTGGCATTAATTCGDAATCC	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32698_Sspa_Oph2007_COI	CNSB-007	1	BE32698_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	GAAGGCCTTCCGATGATC	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32698_Upin_Bot2011_COI	CNSB-007	1	BE32698_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	CTTTAATTACAGCGTTTTTATTGTTGT	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32698_Vgib_Wil2025_COI	CNSB-007	1	BE32698_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	CCCCTCTTTACAAGTTCTTTAGGGT	145.78164
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32699_Asen_Wil2025_COI	CNSB-008	1	BE32699_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	GTTGTTGTTACAACACATGCTCTTTTG	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32699_Cjap_Sim2018_COI	CNSB-008	1	BE32699_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32699_Esin_And2018_Cyb	CNSB-008	1	BE32699_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-008	Cytb	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	ACCCCTCCTCATATCCAACCA	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32699_Mgig_Bot2012_COI	CNSB-008	1	BE32699_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	TCTTATTCGTTGGAGACTTTATAACCCT	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32699_Mjap_Gib2022_COI	CNSB-008	1	BE32699_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	AATTTAATTGTGACTAGGCATGGTCTG	145.78279
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32700_Cmae_Bot2011_COI	CNSB-009	1	BE32700_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	ATGAACAGTCTATCCTCCTTTAG	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32700_Hsan_Wil2021_COI	CNSB-009	1	BE32700_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	CCTGGGCCGGTATAGTAGGT	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32700_Mros_Gib2022_COI	CNSB-009	1	BE32700_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32700_Msal_Bot2012_COI	CNSB-009	1	BE32700_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	GYTAGTTCCRATGATGTTAGCTG	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32700_Mstr_Wil2021_COI	CNSB-009	1	BE32700_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	GTAGTGGTAACTACTCATGCTTTA	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32700_Pvir_Dia2013_COI	CNSB-009	1	BE32700_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	CTTAGTGGCATTAATTCGDAATCC	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32700_Rhar_Sim2018_COI	CNSB-009	1	BE32700_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	CTGCTGCTATTGCTCATG	145.78192
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32702_Aamu_Bax2006_COI	CNSB-011	1	BE32702_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	GCACAACCGGGATCTTTACTTC	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32702_Pcan_Bot2011_IGS	CNSB-011	1	BE32702_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-011	IGS	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	CGTAATCCTCAGTACTGGCTA	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32702_Pper_Dia2013_COI	CNSB-011	1	BE32702_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	CTTAGTGGCATTAATTCGDAATCC	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32702_Sspa_Oph2007_COI	CNSB-011	1	BE32702_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	GAAGGCCTTCCGATGATC	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32702_Upin_Bot2011_COI	CNSB-011	1	BE32702_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	CTTTAATTACAGCGTTTTTATTGTTGT	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32702_Vgib_Wil2025_COI	CNSB-011	1	BE32702_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	CCCCTCTTTACAAGTTCTTTAGGGT	145.78247
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32703_Asen_Wil2025_COI	CNSB-012	1	BE32703_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	GTTGTTGTTACAACACATGCTCTTTTG	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32703_Cjap_Sim2018_COI	CNSB-012	1	BE32703_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32703_Esin_And2018_Cyb	CNSB-012	1	BE32703_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-012	Cytb	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	ACCCCTCCTCATATCCAACCA	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32703_Mgig_Bot2012_COI	CNSB-012	1	BE32703_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	TCTTATTCGTTGGAGACTTTATAACCCT	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32703_Mjap_Gib2022_COI	CNSB-012	1	BE32703_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	AATTTAATTGTGACTAGGCATGGTCTG	145.78168
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32704_Cmae_Bot2011_COI	CNSB-013	1	BE32704_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	ATGAACAGTCTATCCTCCTTTAG	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32704_Hsan_Wil2021_COI	CNSB-013	1	BE32704_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	CCTGGGCCGGTATAGTAGGT	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32704_Mros_Gib2022_COI	CNSB-013	1	BE32704_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32704_Msal_Bot2012_COI	CNSB-013	1	BE32704_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	GYTAGTTCCRATGATGTTAGCTG	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32704_Mstr_Wil2021_COI	CNSB-013	1	BE32704_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	GTAGTGGTAACTACTCATGCTTTA	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32704_Pvir_Dia2013_COI	CNSB-013	1	BE32704_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	CTTAGTGGCATTAATTCGDAATCC	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32704_Rhar_Sim2018_COI	CNSB-013	1	BE32704_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	CTGCTGCTATTGCTCATG	145.78072
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32706_Aamu_Bax2006_COI	CNSB-015	1	BE32706_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	GCACAACCGGGATCTTTACTTC	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32706_Pcan_Bot2011_IGS	CNSB-015	1	BE32706_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-015	IGS	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	CGTAATCCTCAGTACTGGCTA	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32706_Pper_Dia2013_COI	CNSB-015	1	BE32706_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	CTTAGTGGCATTAATTCGDAATCC	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32706_Sspa_Oph2007_COI	CNSB-015	1	BE32706_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	GAAGGCCTTCCGATGATC	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32706_Upin_Bot2011_COI	CNSB-015	1	BE32706_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	CTTTAATTACAGCGTTTTTATTGTTGT	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32706_Vgib_Wil2025_COI	CNSB-015	1	BE32706_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	CCCCTCTTTACAAGTTCTTTAGGGT	145.78114
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32707_Asen_Wil2025_COI	CNSB-016	1	BE32707_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	GTTGTTGTTACAACACATGCTCTTTTG	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32707_Cjap_Sim2018_COI	CNSB-016	1	BE32707_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32707_Esin_And2018_Cyb	CNSB-016	1	BE32707_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-016	Cytb	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	ACCCCTCCTCATATCCAACCA	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32707_Mjap_Gib2022_COI	CNSB-016	1	BE32707_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	AATTTAATTGTGACTAGGCATGGTCTG	145.78074
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32708_Cmae_Bot2011_COI	CNSB-017	1	BE32708_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	ATGAACAGTCTATCCTCCTTTAG	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32708_Hsan_Wil2021_COI	CNSB-017	1	BE32708_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	CCTGGGCCGGTATAGTAGGT	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32708_Mros_Gib2022_COI	CNSB-017	1	BE32708_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32708_Msal_Bot2012_COI	CNSB-017	1	BE32708_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	GYTAGTTCCRATGATGTTAGCTG	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32708_Mstr_Wil2021_COI	CNSB-017	1	BE32708_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	GTAGTGGTAACTACTCATGCTTTA	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32708_Pvir_Dia2013_COI	CNSB-017	1	BE32708_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	CTTAGTGGCATTAATTCGDAATCC	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32708_Rhar_Sim2018_COI	CNSB-017	1	BE32708_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	CTGCTGCTATTGCTCATG	145.7822
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32710_Asen_Wil2025_COI	CNSB-019	1	BE32710_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	GTTGTTGTTACAACACATGCTCTTTTG	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32710_Cjap_Sim2018_COI	CNSB-019	1	BE32710_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32710_Esin_And2018_Cyb	CNSB-019	1	BE32710_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-019	Cytb	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	ACCCCTCCTCATATCCAACCA	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32710_Mgig_Bot2012_COI	CNSB-019	1	BE32710_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	TCTTATTCGTTGGAGACTTTATAACCCT	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32710_Mjap_Gib2022_COI	CNSB-019	1	BE32710_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	AATTTAATTGTGACTAGGCATGGTCTG	145.78283
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32711_Cmae_Bot2011_COI	CNSB-020	1	BE32711_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	ATGAACAGTCTATCCTCCTTTAG	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32711_Hsan_Wil2021_COI	CNSB-020	1	BE32711_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	CCTGGGCCGGTATAGTAGGT	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32711_Mros_Gib2022_COI	CNSB-020	1	BE32711_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32711_Msal_Bot2012_COI	CNSB-020	1	BE32711_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	GYTAGTTCCRATGATGTTAGCTG	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32711_Mstr_Wil2021_COI	CNSB-020	1	BE32711_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	GTAGTGGTAACTACTCATGCTTTA	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32711_Pvir_Dia2013_COI	CNSB-020	1	BE32711_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	CTTAGTGGCATTAATTCGDAATCC	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32711_Rhar_Sim2018_COI	CNSB-020	1	BE32711_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	CTGCTGCTATTGCTCATG	145.78186
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32713_Aamu_Bax2006_COI	CNSB-022	1	BE32713_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	GCACAACCGGGATCTTTACTTC	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32713_Pcan_Bot2011_IGS	CNSB-022	1	BE32713_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-022	IGS	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	CGTAATCCTCAGTACTGGCTA	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32713_Pper_Dia2013_COI	CNSB-022	1	BE32713_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	CTTAGTGGCATTAATTCGDAATCC	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32713_Sspa_Oph2007_COI	CNSB-022	1	BE32713_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	GAAGGCCTTCCGATGATC	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32713_Upin_Bot2011_COI	CNSB-022	1	BE32713_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	CTTTAATTACAGCGTTTTTATTGTTGT	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32713_Vgib_Wil2025_COI	CNSB-022	1	BE32713_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	CCCCTCTTTACAAGTTCTTTAGGGT	145.78269
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32714_Asen_Wil2025_COI	CNSB-023	1	BE32714_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	GTTGTTGTTACAACACATGCTCTTTTG	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32714_Cjap_Sim2018_COI	CNSB-023	1	BE32714_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32714_Esin_And2018_Cyb	CNSB-023	1	BE32714_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-023	Cytb	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	ACCCCTCCTCATATCCAACCA	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32714_Mjap_Gib2022_COI	CNSB-023	1	BE32714_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	AATTTAATTGTGACTAGGCATGGTCTG	145.78405
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32715_Cmae_Bot2011_COI	CNSB-024	1	BE32715_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	ATGAACAGTCTATCCTCCTTTAG	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32715_Hsan_Wil2021_COI	CNSB-024	1	BE32715_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	CCTGGGCCGGTATAGTAGGT	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32715_Mros_Gib2022_COI	CNSB-024	1	BE32715_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32715_Msal_Bot2012_COI	CNSB-024	1	BE32715_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	GYTAGTTCCRATGATGTTAGCTG	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32715_Mstr_Wil2021_COI	CNSB-024	1	BE32715_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	GTAGTGGTAACTACTCATGCTTTA	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32715_Pvir_Dia2013_COI	CNSB-024	1	BE32715_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	CTTAGTGGCATTAATTCGDAATCC	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32715_Rhar_Sim2018_COI	CNSB-024	1	BE32715_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	CTGCTGCTATTGCTCATG	145.78184
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32717_Aamu_Bax2006_COI	CNSB-026	1	BE32717_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	GCACAACCGGGATCTTTACTTC	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32717_Pcan_Bot2011_IGS	CNSB-026	1	BE32717_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-026	IGS	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	CGTAATCCTCAGTACTGGCTA	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32717_Pper_Dia2013_COI	CNSB-026	1	BE32717_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	CTTAGTGGCATTAATTCGDAATCC	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32717_Sspa_Oph2007_COI	CNSB-026	1	BE32717_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	GAAGGCCTTCCGATGATC	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32717_Upin_Bot2011_COI	CNSB-026	1	BE32717_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	CTTTAATTACAGCGTTTTTATTGTTGT	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32717_Vgib_Wil2025_COI	CNSB-026	1	BE32717_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	CCCCTCTTTACAAGTTCTTTAGGGT	145.78234
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32718_Asen_Wil2025_COI	CNSB-027	1	BE32718_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	GTTGTTGTTACAACACATGCTCTTTTG	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32718_Cjap_Sim2018_COI	CNSB-027	1	BE32718_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32718_Esin_And2018_Cyb	CNSB-027	1	BE32718_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-027	Cytb	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	ACCCCTCCTCATATCCAACCA	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32718_Mgig_Bot2012_COI	CNSB-027	1	BE32718_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	TCTTATTCGTTGGAGACTTTATAACCCT	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32718_Mjap_Gib2022_COI	CNSB-027	1	BE32718_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	AATTTAATTGTGACTAGGCATGGTCTG	145.78385
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32719_Cmae_Bot2011_COI	CNSB-028	1	BE32719_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	ATGAACAGTCTATCCTCCTTTAG	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32719_Hsan_Wil2021_COI	CNSB-028	1	BE32719_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	CCTGGGCCGGTATAGTAGGT	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32719_Mros_Gib2022_COI	CNSB-028	1	BE32719_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32719_Msal_Bot2012_COI	CNSB-028	1	BE32719_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	GYTAGTTCCRATGATGTTAGCTG	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32719_Mstr_Wil2021_COI	CNSB-028	1	BE32719_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	GTAGTGGTAACTACTCATGCTTTA	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32719_Pvir_Dia2013_COI	CNSB-028	1	BE32719_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	CTTAGTGGCATTAATTCGDAATCC	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32719_Rhar_Sim2018_COI	CNSB-028	1	BE32719_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	CTGCTGCTATTGCTCATG	145.78334
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32720_Aamu_Bax2006_COI	CNSB-029	1	BE32720_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	GCACAACCGGGATCTTTACTTC	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32720_Pcan_Bot2011_IGS	CNSB-029	1	BE32720_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-029	IGS	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	CGTAATCCTCAGTACTGGCTA	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32720_Pper_Dia2013_COI	CNSB-029	1	BE32720_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	CTTAGTGGCATTAATTCGDAATCC	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32720_Sspa_Oph2007_COI	CNSB-029	1	BE32720_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	GAAGGCCTTCCGATGATC	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32720_Upin_Bot2011_COI	CNSB-029	1	BE32720_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	CTTTAATTACAGCGTTTTTATTGTTGT	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32720_Vgib_Wil2025_COI	CNSB-029	1	BE32720_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	CCCCTCTTTACAAGTTCTTTAGGGT	145.78461
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32721_Asen_Wil2025_COI	CNSB-030	1	BE32721_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	GTTGTTGTTACAACACATGCTCTTTTG	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32721_Cjap_Sim2018_COI	CNSB-030	1	BE32721_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32721_Esin_And2018_Cyb	CNSB-030	1	BE32721_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-030	Cytb	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	ACCCCTCCTCATATCCAACCA	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32721_Mgig_Bot2012_COI	CNSB-030	1	BE32721_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	TCTTATTCGTTGGAGACTTTATAACCCT	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32721_Mjap_Gib2022_COI	CNSB-030	1	BE32721_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	AATTTAATTGTGACTAGGCATGGTCTG	145.78369
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32722_Cmae_Bot2011_COI	CNSB-031	1	BE32722_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	ATGAACAGTCTATCCTCCTTTAG	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32722_Hsan_Wil2021_COI	CNSB-031	1	BE32722_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	CCTGGGCCGGTATAGTAGGT	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32722_Mros_Gib2022_COI	CNSB-031	1	BE32722_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32722_Msal_Bot2012_COI	CNSB-031	1	BE32722_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	GYTAGTTCCRATGATGTTAGCTG	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32722_Mstr_Wil2021_COI	CNSB-031	1	BE32722_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	GTAGTGGTAACTACTCATGCTTTA	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32722_Pvir_Dia2013_COI	CNSB-031	1	BE32722_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	CTTAGTGGCATTAATTCGDAATCC	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32722_Rhar_Sim2018_COI	CNSB-031	1	BE32722_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	CTGCTGCTATTGCTCATG	145.78513
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32724_Aamu_Bax2006_COI	CNSB-033	1	BE32724_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	GCACAACCGGGATCTTTACTTC	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32724_Pcan_Bot2011_IGS	CNSB-033	1	BE32724_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-033	IGS	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	CGTAATCCTCAGTACTGGCTA	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32724_Pper_Dia2013_COI	CNSB-033	1	BE32724_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	CTTAGTGGCATTAATTCGDAATCC	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32724_Sspa_Oph2007_COI	CNSB-033	1	BE32724_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	GAAGGCCTTCCGATGATC	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32724_Upin_Bot2011_COI	CNSB-033	1	BE32724_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	CTTTAATTACAGCGTTTTTATTGTTGT	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32724_Vgib_Wil2025_COI	CNSB-033	1	BE32724_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	CCCCTCTTTACAAGTTCTTTAGGGT	145.78517
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32725_Asen_Wil2025_COI	CNSB-034	1	BE32725_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	GTTGTTGTTACAACACATGCTCTTTTG	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32725_Cjap_Sim2018_COI	CNSB-034	1	BE32725_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32725_Esin_And2018_Cyb	CNSB-034	1	BE32725_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-034	Cytb	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	ACCCCTCCTCATATCCAACCA	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32725_Mgig_Bot2012_COI	CNSB-034	1	BE32725_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	TCTTATTCGTTGGAGACTTTATAACCCT	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32725_Mjap_Gib2022_COI	CNSB-034	1	BE32725_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	AATTTAATTGTGACTAGGCATGGTCTG	145.78422
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32726_Cmae_Bot2011_COI	CNSB-035	1	BE32726_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	ATGAACAGTCTATCCTCCTTTAG	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32726_Hsan_Wil2021_COI	CNSB-035	1	BE32726_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	CCTGGGCCGGTATAGTAGGT	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32726_Mros_Gib2022_COI	CNSB-035	1	BE32726_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32726_Msal_Bot2012_COI	CNSB-035	1	BE32726_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	GYTAGTTCCRATGATGTTAGCTG	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32726_Mstr_Wil2021_COI	CNSB-035	1	BE32726_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	GTAGTGGTAACTACTCATGCTTTA	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32726_Pvir_Dia2013_COI	CNSB-035	1	BE32726_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	CTTAGTGGCATTAATTCGDAATCC	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32726_Rhar_Sim2018_COI	CNSB-035	1	BE32726_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	CTGCTGCTATTGCTCATG	145.78546
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32733_Cmae_Bot2011_COI	TSVB-001	1	BE32733_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	ATGAACAGTCTATCCTCCTTTAG	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32733_Hsan_Wil2021_COI	TSVB-001	1	BE32733_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	CCTGGGCCGGTATAGTAGGT	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32733_Mros_Gib2022_COI	TSVB-001	1	BE32733_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32733_Msal_Bot2012_COI	TSVB-001	1	BE32733_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	GYTAGTTCCRATGATGTTAGCTG	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32733_Mstr_Wil2021_COI	TSVB-001	1	BE32733_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	GTAGTGGTAACTACTCATGCTTTA	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32733_Pvir_Dia2013_COI	TSVB-001	1	BE32733_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	CTTAGTGGCATTAATTCGDAATCC	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32733_Rhar_Sim2018_COI	TSVB-001	1	BE32733_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	CTGCTGCTATTGCTCATG	146.84256
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32735_Aamu_Bax2006_COI	TSVB-003	1	BE32735_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	GCACAACCGGGATCTTTACTTC	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32735_Pcan_Bot2011_IGS	TSVB-003	1	BE32735_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-003	IGS	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	CGTAATCCTCAGTACTGGCTA	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32735_Pper_Dia2013_COI	TSVB-003	1	BE32735_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	CTTAGTGGCATTAATTCGDAATCC	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32735_Sspa_Oph2007_COI	TSVB-003	1	BE32735_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	GAAGGCCTTCCGATGATC	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32735_Upin_Bot2011_COI	TSVB-003	1	BE32735_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	CTTTAATTACAGCGTTTTTATTGTTGT	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32735_Vgib_Wil2025_COI	TSVB-003	1	BE32735_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	CCCCTCTTTACAAGTTCTTTAGGGT	146.84007
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32736_Asen_Wil2025_COI	TSVB-004	1	BE32736_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	GTTGTTGTTACAACACATGCTCTTTTG	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32736_Cjap_Sim2018_COI	TSVB-004	1	BE32736_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32736_Esin_And2018_Cyb	TSVB-004	1	BE32736_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-004	Cytb	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	ACCCCTCCTCATATCCAACCA	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32736_Mgig_Bot2012_COI	TSVB-004	1	BE32736_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	TCTTATTCGTTGGAGACTTTATAACCCT	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32736_Mjap_Gib2022_COI	TSVB-004	1	BE32736_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	AATTTAATTGTGACTAGGCATGGTCTG	146.8404
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32737_Cmae_Bot2011_COI	TSVB-005	1	BE32737_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	ATGAACAGTCTATCCTCCTTTAG	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32737_Hsan_Wil2021_COI	TSVB-005	1	BE32737_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	CCTGGGCCGGTATAGTAGGT	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32737_Mros_Gib2022_COI	TSVB-005	1	BE32737_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32737_Msal_Bot2012_COI	TSVB-005	1	BE32737_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	GYTAGTTCCRATGATGTTAGCTG	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32737_Mstr_Wil2021_COI	TSVB-005	1	BE32737_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	GTAGTGGTAACTACTCATGCTTTA	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32737_Pvir_Dia2013_COI	TSVB-005	1	BE32737_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	CTTAGTGGCATTAATTCGDAATCC	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32737_Rhar_Sim2018_COI	TSVB-005	1	BE32737_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	CTGCTGCTATTGCTCATG	146.84199
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32739_Aamu_Bax2006_COI	TSVB-007	1	BE32739_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	GCACAACCGGGATCTTTACTTC	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32739_Pcan_Bot2011_IGS	TSVB-007	1	BE32739_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-007	IGS	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	CGTAATCCTCAGTACTGGCTA	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32739_Pper_Dia2013_COI	TSVB-007	1	BE32739_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	CTTAGTGGCATTAATTCGDAATCC	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32739_Sspa_Oph2007_COI	TSVB-007	1	BE32739_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	GAAGGCCTTCCGATGATC	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32739_Upin_Bot2011_COI	TSVB-007	1	BE32739_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	CTTTAATTACAGCGTTTTTATTGTTGT	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32739_Vgib_Wil2025_COI	TSVB-007	1	BE32739_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	CCCCTCTTTACAAGTTCTTTAGGGT	146.8396
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32740_Cmae_Bot2011_COI	TSVB-008	1	BE32740_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	ATGAACAGTCTATCCTCCTTTAG	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32740_Hsan_Wil2021_COI	TSVB-008	1	BE32740_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	CCTGGGCCGGTATAGTAGGT	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32740_Mros_Gib2022_COI	TSVB-008	1	BE32740_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32740_Msal_Bot2012_COI	TSVB-008	1	BE32740_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	GYTAGTTCCRATGATGTTAGCTG	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32740_Mstr_Wil2021_COI	TSVB-008	1	BE32740_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	GTAGTGGTAACTACTCATGCTTTA	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32740_Pvir_Dia2013_COI	TSVB-008	1	BE32740_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	CTTAGTGGCATTAATTCGDAATCC	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32740_Rhar_Sim2018_COI	TSVB-008	1	BE32740_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	CTGCTGCTATTGCTCATG	146.83793
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32742_Aamu_Bax2006_COI	TSVB-010	1	BE32742_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	GCACAACCGGGATCTTTACTTC	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32742_Pcan_Bot2011_IGS	TSVB-010	1	BE32742_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-010	IGS	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	CGTAATCCTCAGTACTGGCTA	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32742_Pper_Dia2013_COI	TSVB-010	1	BE32742_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	CTTAGTGGCATTAATTCGDAATCC	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32742_Sspa_Oph2007_COI	TSVB-010	1	BE32742_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	GAAGGCCTTCCGATGATC	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32742_Upin_Bot2011_COI	TSVB-010	1	BE32742_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	CTTTAATTACAGCGTTTTTATTGTTGT	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32742_Vgib_Wil2025_COI	TSVB-010	1	BE32742_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	CCCCTCTTTACAAGTTCTTTAGGGT	146.83856
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32743_Asen_Wil2025_COI	TSVB-011	1	BE32743_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	GTTGTTGTTACAACACATGCTCTTTTG	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32743_Cjap_Sim2018_COI	TSVB-011	1	BE32743_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32743_Esin_And2018_Cyb	TSVB-011	1	BE32743_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-011	Cytb	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	ACCCCTCCTCATATCCAACCA	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32743_Mgig_Bot2012_COI	TSVB-011	1	BE32743_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	TCTTATTCGTTGGAGACTTTATAACCCT	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32743_Mjap_Gib2022_COI	TSVB-011	1	BE32743_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	AATTTAATTGTGACTAGGCATGGTCTG	146.83706
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32744_Cmae_Bot2011_COI	TSVB-012	1	BE32744_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	ATGAACAGTCTATCCTCCTTTAG	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32744_Hsan_Wil2021_COI	TSVB-012	1	BE32744_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	CCTGGGCCGGTATAGTAGGT	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32744_Mros_Gib2022_COI	TSVB-012	1	BE32744_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32744_Msal_Bot2012_COI	TSVB-012	1	BE32744_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	GYTAGTTCCRATGATGTTAGCTG	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32744_Mstr_Wil2021_COI	TSVB-012	1	BE32744_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	GTAGTGGTAACTACTCATGCTTTA	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32744_Pvir_Dia2013_COI	TSVB-012	1	BE32744_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	CTTAGTGGCATTAATTCGDAATCC	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32744_Rhar_Sim2018_COI	TSVB-012	1	BE32744_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	CTGCTGCTATTGCTCATG	146.83539
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32746_Aamu_Bax2006_COI	TSVB-014	1	BE32746_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	GCACAACCGGGATCTTTACTTC	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32746_Pcan_Bot2011_IGS	TSVB-014	1	BE32746_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-014	IGS	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	CGTAATCCTCAGTACTGGCTA	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32746_Pper_Dia2013_COI	TSVB-014	1	BE32746_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	CTTAGTGGCATTAATTCGDAATCC	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32746_Sspa_Oph2007_COI	TSVB-014	1	BE32746_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	GAAGGCCTTCCGATGATC	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32746_Upin_Bot2011_COI	TSVB-014	1	BE32746_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	CTTTAATTACAGCGTTTTTATTGTTGT	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32746_Vgib_Wil2025_COI	TSVB-014	1	BE32746_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	CCCCTCTTTACAAGTTCTTTAGGGT	146.83263
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32747_Asen_Wil2025_COI	TSVB-015	1	BE32747_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	GTTGTTGTTACAACACATGCTCTTTTG	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32747_Cjap_Sim2018_COI	TSVB-015	1	BE32747_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32747_Esin_And2018_Cyb	TSVB-015	1	BE32747_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-015	Cytb	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	ACCCCTCCTCATATCCAACCA	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32747_Mgig_Bot2012_COI	TSVB-015	1	BE32747_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	TCTTATTCGTTGGAGACTTTATAACCCT	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32747_Mjap_Gib2022_COI	TSVB-015	1	BE32747_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	AATTTAATTGTGACTAGGCATGGTCTG	146.83915
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32748_Cmae_Bot2011_COI	TSVB-016	1	BE32748_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	ATGAACAGTCTATCCTCCTTTAG	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32748_Hsan_Wil2021_COI	TSVB-016	1	BE32748_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	CCTGGGCCGGTATAGTAGGT	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32748_Mros_Gib2022_COI	TSVB-016	1	BE32748_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32748_Msal_Bot2012_COI	TSVB-016	1	BE32748_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	GYTAGTTCCRATGATGTTAGCTG	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32748_Mstr_Wil2021_COI	TSVB-016	1	BE32748_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	GTAGTGGTAACTACTCATGCTTTA	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32748_Pvir_Dia2013_COI	TSVB-016	1	BE32748_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	CTTAGTGGCATTAATTCGDAATCC	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32748_Rhar_Sim2018_COI	TSVB-016	1	BE32748_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	CTGCTGCTATTGCTCATG	146.83762
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32750_Asen_Wil2025_COI	TSVB-018	1	BE32750_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	GTTGTTGTTACAACACATGCTCTTTTG	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32750_Cjap_Sim2018_COI	TSVB-018	1	BE32750_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32750_Esin_And2018_Cyb	TSVB-018	1	BE32750_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-018	Cytb	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	ACCCCTCCTCATATCCAACCA	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32750_Mgig_Bot2012_COI	TSVB-018	1	BE32750_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	TCTTATTCGTTGGAGACTTTATAACCCT	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32750_Mjap_Gib2022_COI	TSVB-018	1	BE32750_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	AATTTAATTGTGACTAGGCATGGTCTG	146.83444
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32751_Cmae_Bot2011_COI	TSVB-019	1	BE32751_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	ATGAACAGTCTATCCTCCTTTAG	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32751_Hsan_Wil2021_COI	TSVB-019	1	BE32751_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	CCTGGGCCGGTATAGTAGGT	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32751_Mros_Gib2022_COI	TSVB-019	1	BE32751_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32751_Msal_Bot2012_COI	TSVB-019	1	BE32751_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	GYTAGTTCCRATGATGTTAGCTG	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32751_Mstr_Wil2021_COI	TSVB-019	1	BE32751_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	GTAGTGGTAACTACTCATGCTTTA	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32751_Pvir_Dia2013_COI	TSVB-019	1	BE32751_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	CTTAGTGGCATTAATTCGDAATCC	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32751_Rhar_Sim2018_COI	TSVB-019	1	BE32751_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	CTGCTGCTATTGCTCATG	146.83331
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32753_Aamu_Bax2006_COI	TSVB-021	1	BE32753_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	GCACAACCGGGATCTTTACTTC	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32753_Pcan_Bot2011_IGS	TSVB-021	1	BE32753_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-021	IGS	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	CGTAATCCTCAGTACTGGCTA	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32753_Pper_Dia2013_COI	TSVB-021	1	BE32753_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	CTTAGTGGCATTAATTCGDAATCC	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32753_Sspa_Oph2007_COI	TSVB-021	1	BE32753_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	GAAGGCCTTCCGATGATC	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32753_Upin_Bot2011_COI	TSVB-021	1	BE32753_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	CTTTAATTACAGCGTTTTTATTGTTGT	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32753_Vgib_Wil2025_COI	TSVB-021	1	BE32753_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	CCCCTCTTTACAAGTTCTTTAGGGT	146.83614
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32754_Asen_Wil2025_COI	TSVB-022	1	BE32754_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	GTTGTTGTTACAACACATGCTCTTTTG	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32754_Cjap_Sim2018_COI	TSVB-022	1	BE32754_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32754_Esin_And2018_Cyb	TSVB-022	1	BE32754_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-022	Cytb	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	ACCCCTCCTCATATCCAACCA	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32754_Mgig_Bot2012_COI	TSVB-022	1	BE32754_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	TCTTATTCGTTGGAGACTTTATAACCCT	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32754_Mjap_Gib2022_COI	TSVB-022	1	BE32754_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	AATTTAATTGTGACTAGGCATGGTCTG	146.83487
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32755_Cmae_Bot2011_COI	TSVB-023	1	BE32755_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	ATGAACAGTCTATCCTCCTTTAG	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32755_Hsan_Wil2021_COI	TSVB-023	1	BE32755_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	CCTGGGCCGGTATAGTAGGT	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32755_Mros_Gib2022_COI	TSVB-023	1	BE32755_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32755_Msal_Bot2012_COI	TSVB-023	1	BE32755_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	GYTAGTTCCRATGATGTTAGCTG	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32755_Mstr_Wil2021_COI	TSVB-023	1	BE32755_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	GTAGTGGTAACTACTCATGCTTTA	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32755_Pvir_Dia2013_COI	TSVB-023	1	BE32755_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	CTTAGTGGCATTAATTCGDAATCC	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32755_Rhar_Sim2018_COI	TSVB-023	1	BE32755_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	CTGCTGCTATTGCTCATG	146.83892
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32757_Aamu_Bax2006_COI	TSVB-025	1	BE32757_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	GCACAACCGGGATCTTTACTTC	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32757_Pcan_Bot2011_IGS	TSVB-025	1	BE32757_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-025	IGS	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	CGTAATCCTCAGTACTGGCTA	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32757_Pper_Dia2013_COI	TSVB-025	1	BE32757_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	CTTAGTGGCATTAATTCGDAATCC	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32757_Sspa_Oph2007_COI	TSVB-025	1	BE32757_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	GAAGGCCTTCCGATGATC	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32757_Upin_Bot2011_COI	TSVB-025	1	BE32757_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	CTTTAATTACAGCGTTTTTATTGTTGT	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32757_Vgib_Wil2025_COI	TSVB-025	1	BE32757_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	CCCCTCTTTACAAGTTCTTTAGGGT	146.83361
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32758_Asen_Wil2025_COI	TSVB-026	1	BE32758_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	GTTGTTGTTACAACACATGCTCTTTTG	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32758_Cjap_Sim2018_COI	TSVB-026	1	BE32758_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32758_Esin_And2018_Cyb	TSVB-026	1	BE32758_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-026	Cytb	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	ACCCCTCCTCATATCCAACCA	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32758_Mgig_Bot2012_COI	TSVB-026	1	BE32758_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	TCTTATTCGTTGGAGACTTTATAACCCT	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32758_Mjap_Gib2022_COI	TSVB-026	1	BE32758_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	AATTTAATTGTGACTAGGCATGGTCTG	146.83467
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32759_Cmae_Bot2011_COI	TSVB-027	1	BE32759_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	ATGAACAGTCTATCCTCCTTTAG	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32759_Hsan_Wil2021_COI	TSVB-027	1	BE32759_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	CCTGGGCCGGTATAGTAGGT	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32759_Mros_Gib2022_COI	TSVB-027	1	BE32759_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32759_Msal_Bot2012_COI	TSVB-027	1	BE32759_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	GYTAGTTCCRATGATGTTAGCTG	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32759_Mstr_Wil2021_COI	TSVB-027	1	BE32759_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	GTAGTGGTAACTACTCATGCTTTA	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32759_Pvir_Dia2013_COI	TSVB-027	1	BE32759_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	CTTAGTGGCATTAATTCGDAATCC	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32759_Rhar_Sim2018_COI	TSVB-027	1	BE32759_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	CTGCTGCTATTGCTCATG	146.83533
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32760_Aamu_Bax2006_COI	TSVB-028	1	BE32760_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	GCACAACCGGGATCTTTACTTC	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32760_Pcan_Bot2011_IGS	TSVB-028	1	BE32760_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-028	IGS	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	CGTAATCCTCAGTACTGGCTA	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32760_Pper_Dia2013_COI	TSVB-028	1	BE32760_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	CTTAGTGGCATTAATTCGDAATCC	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32760_Sspa_Oph2007_COI	TSVB-028	1	BE32760_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	GAAGGCCTTCCGATGATC	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32760_Upin_Bot2011_COI	TSVB-028	1	BE32760_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	CTTTAATTACAGCGTTTTTATTGTTGT	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32760_Vgib_Wil2025_COI	TSVB-028	1	BE32760_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	CCCCTCTTTACAAGTTCTTTAGGGT	146.83458
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32761_Asen_Wil2025_COI	TSVB-029	1	BE32761_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	GTTGTTGTTACAACACATGCTCTTTTG	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32761_Cjap_Sim2018_COI	TSVB-029	1	BE32761_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32761_Esin_And2018_Cyb	TSVB-029	1	BE32761_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-029	Cytb	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	ACCCCTCCTCATATCCAACCA	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32761_Mgig_Bot2012_COI	TSVB-029	1	BE32761_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	TCTTATTCGTTGGAGACTTTATAACCCT	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32761_Mjap_Gib2022_COI	TSVB-029	1	BE32761_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	AATTTAATTGTGACTAGGCATGGTCTG	146.83352
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32762_Cmae_Bot2011_COI	TSVB-030	1	BE32762_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	ATGAACAGTCTATCCTCCTTTAG	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32762_Mros_Gib2022_COI	TSVB-030	1	BE32762_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32762_Msal_Bot2012_COI	TSVB-030	1	BE32762_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	GYTAGTTCCRATGATGTTAGCTG	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32762_Mstr_Wil2021_COI	TSVB-030	1	BE32762_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	GTAGTGGTAACTACTCATGCTTTA	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32762_Pvir_Dia2013_COI	TSVB-030	1	BE32762_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	CTTAGTGGCATTAATTCGDAATCC	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32762_Rhar_Sim2018_COI	TSVB-030	1	BE32762_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	CTGCTGCTATTGCTCATG	146.83326
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32764_Aamu_Bax2006_COI	TSVB-032	1	BE32764_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	GCACAACCGGGATCTTTACTTC	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32764_Pcan_Bot2011_IGS	TSVB-032	1	BE32764_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-032	IGS	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	CGTAATCCTCAGTACTGGCTA	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32764_Pper_Dia2013_COI	TSVB-032	1	BE32764_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	CTTAGTGGCATTAATTCGDAATCC	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32764_Sspa_Oph2007_COI	TSVB-032	1	BE32764_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	GAAGGCCTTCCGATGATC	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32764_Upin_Bot2011_COI	TSVB-032	1	BE32764_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	CTTTAATTACAGCGTTTTTATTGTTGT	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32764_Vgib_Wil2025_COI	TSVB-032	1	BE32764_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	CCCCTCTTTACAAGTTCTTTAGGGT	146.83404
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32765_Asen_Wil2025_COI	TSVB-033	1	BE32765_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	GTTGTTGTTACAACACATGCTCTTTTG	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32765_Cjap_Sim2018_COI	TSVB-033	1	BE32765_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32765_Esin_And2018_Cyb	TSVB-033	1	BE32765_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-033	Cytb	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	ACCCCTCCTCATATCCAACCA	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32765_Mgig_Bot2012_COI	TSVB-033	1	BE32765_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	TCTTATTCGTTGGAGACTTTATAACCCT	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32765_Mjap_Gib2022_COI	TSVB-033	1	BE32765_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	AATTTAATTGTGACTAGGCATGGTCTG	146.83413
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32766_Cmae_Bot2011_COI	TSVB-034	1	BE32766_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	ATGAACAGTCTATCCTCCTTTAG	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32766_Hsan_Wil2021_COI	TSVB-034	1	BE32766_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	CCTGGGCCGGTATAGTAGGT	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32766_Mros_Gib2022_COI	TSVB-034	1	BE32766_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32766_Msal_Bot2012_COI	TSVB-034	1	BE32766_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	GYTAGTTCCRATGATGTTAGCTG	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32766_Mstr_Wil2021_COI	TSVB-034	1	BE32766_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	GTAGTGGTAACTACTCATGCTTTA	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32766_Pvir_Dia2013_COI	TSVB-034	1	BE32766_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	CTTAGTGGCATTAATTCGDAATCC	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32766_Rhar_Sim2018_COI	TSVB-034	1	BE32766_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	CTGCTGCTATTGCTCATG	146.83207
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32775_Aamu_Bax2006_COI	DRWB-002	1	BE32775_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	GCACAACCGGGATCTTTACTTC	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32775_Pcan_Bot2011_IGS	DRWB-002	1	BE32775_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-002	IGS	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	CGTAATCCTCAGTACTGGCTA	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32775_Pper_Dia2013_COI	DRWB-002	1	BE32775_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	CTTAGTGGCATTAATTCGDAATCC	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32775_Sspa_Oph2007_COI	DRWB-002	1	BE32775_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	GAAGGCCTTCCGATGATC	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32775_Upin_Bot2011_COI	DRWB-002	1	BE32775_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	CTTTAATTACAGCGTTTTTATTGTTGT	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32775_Vgib_Wil2025_COI	DRWB-002	1	BE32775_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	CCCCTCTTTACAAGTTCTTTAGGGT	130.90463
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32776_Asen_Wil2025_COI	DRWB-003	1	BE32776_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	GTTGTTGTTACAACACATGCTCTTTTG	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32776_Cjap_Sim2018_COI	DRWB-003	1	BE32776_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32776_Esin_And2018_Cyb	DRWB-003	1	BE32776_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-003	Cytb	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	ACCCCTCCTCATATCCAACCA	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32776_Mgig_Bot2012_COI	DRWB-003	1	BE32776_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	TCTTATTCGTTGGAGACTTTATAACCCT	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32776_Mjap_Gib2022_COI	DRWB-003	1	BE32776_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	AATTTAATTGTGACTAGGCATGGTCTG	130.90357
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32777_Cmae_Bot2011_COI	DRWB-004	1	BE32777_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	ATGAACAGTCTATCCTCCTTTAG	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32777_Hsan_Wil2021_COI	DRWB-004	1	BE32777_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	CCTGGGCCGGTATAGTAGGT	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32777_Mros_Gib2022_COI	DRWB-004	1	BE32777_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32777_Msal_Bot2012_COI	DRWB-004	1	BE32777_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	GYTAGTTCCRATGATGTTAGCTG	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32777_Mstr_Wil2021_COI	DRWB-004	1	BE32777_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	GTAGTGGTAACTACTCATGCTTTA	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32777_Pvir_Dia2013_COI	DRWB-004	1	BE32777_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	CTTAGTGGCATTAATTCGDAATCC	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32777_Rhar_Sim2018_COI	DRWB-004	1	BE32777_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	CTGCTGCTATTGCTCATG	130.9043
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32779_Aamu_Bax2006_COI	DRWB-006	1	BE32779_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	GCACAACCGGGATCTTTACTTC	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32779_Pcan_Bot2011_IGS	DRWB-006	1	BE32779_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-006	IGS	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	CGTAATCCTCAGTACTGGCTA	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32779_Pper_Dia2013_COI	DRWB-006	1	BE32779_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	CTTAGTGGCATTAATTCGDAATCC	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32779_Sspa_Oph2007_COI	DRWB-006	1	BE32779_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	GAAGGCCTTCCGATGATC	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32779_Upin_Bot2011_COI	DRWB-006	1	BE32779_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	CTTTAATTACAGCGTTTTTATTGTTGT	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32779_Vgib_Wil2025_COI	DRWB-006	1	BE32779_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	CCCCTCTTTACAAGTTCTTTAGGGT	130.90367
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32780_Cmae_Bot2011_COI	DRWB-007	1	BE32780_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	ATGAACAGTCTATCCTCCTTTAG	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32780_Hsan_Wil2021_COI	DRWB-007	1	BE32780_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	CCTGGGCCGGTATAGTAGGT	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32780_Mros_Gib2022_COI	DRWB-007	1	BE32780_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32780_Msal_Bot2012_COI	DRWB-007	1	BE32780_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	GYTAGTTCCRATGATGTTAGCTG	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32780_Mstr_Wil2021_COI	DRWB-007	1	BE32780_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	GTAGTGGTAACTACTCATGCTTTA	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32780_Pvir_Dia2013_COI	DRWB-007	1	BE32780_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	CTTAGTGGCATTAATTCGDAATCC	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32780_Rhar_Sim2018_COI	DRWB-007	1	BE32780_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	CTGCTGCTATTGCTCATG	130.90486
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32782_Aamu_Bax2006_COI	DRWB-009	1	BE32782_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	GCACAACCGGGATCTTTACTTC	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32782_Pcan_Bot2011_IGS	DRWB-009	1	BE32782_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-009	IGS	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	CGTAATCCTCAGTACTGGCTA	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32782_Pper_Dia2013_COI	DRWB-009	1	BE32782_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	CTTAGTGGCATTAATTCGDAATCC	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32782_Sspa_Oph2007_COI	DRWB-009	1	BE32782_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	GAAGGCCTTCCGATGATC	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32782_Upin_Bot2011_COI	DRWB-009	1	BE32782_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	CTTTAATTACAGCGTTTTTATTGTTGT	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32782_Vgib_Wil2025_COI	DRWB-009	1	BE32782_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	CCCCTCTTTACAAGTTCTTTAGGGT	130.90555
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32783_Asen_Wil2025_COI	DRWB-010	1	BE32783_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	GTTGTTGTTACAACACATGCTCTTTTG	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32783_Cjap_Sim2018_COI	DRWB-010	1	BE32783_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32783_Esin_And2018_Cyb	DRWB-010	1	BE32783_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-010	Cytb	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	ACCCCTCCTCATATCCAACCA	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32783_Mgig_Bot2012_COI	DRWB-010	1	BE32783_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	TCTTATTCGTTGGAGACTTTATAACCCT	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32783_Mjap_Gib2022_COI	DRWB-010	1	BE32783_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	AATTTAATTGTGACTAGGCATGGTCTG	130.90673
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32784_Cmae_Bot2011_COI	DRWB-011	1	BE32784_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	ATGAACAGTCTATCCTCCTTTAG	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32784_Hsan_Wil2021_COI	DRWB-011	1	BE32784_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	CCTGGGCCGGTATAGTAGGT	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32784_Mros_Gib2022_COI	DRWB-011	1	BE32784_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32784_Msal_Bot2012_COI	DRWB-011	1	BE32784_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	GYTAGTTCCRATGATGTTAGCTG	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32784_Mstr_Wil2021_COI	DRWB-011	1	BE32784_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	GTAGTGGTAACTACTCATGCTTTA	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32784_Pvir_Dia2013_COI	DRWB-011	1	BE32784_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	CTTAGTGGCATTAATTCGDAATCC	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32784_Rhar_Sim2018_COI	DRWB-011	1	BE32784_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	CTGCTGCTATTGCTCATG	130.90786
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32786_Aamu_Bax2006_COI	DRWB-013	1	BE32786_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	GCACAACCGGGATCTTTACTTC	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32786_Pcan_Bot2011_IGS	DRWB-013	1	BE32786_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-013	IGS	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	CGTAATCCTCAGTACTGGCTA	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32786_Pper_Dia2013_COI	DRWB-013	1	BE32786_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	CTTAGTGGCATTAATTCGDAATCC	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32786_Sspa_Oph2007_COI	DRWB-013	1	BE32786_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	GAAGGCCTTCCGATGATC	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32786_Upin_Bot2011_COI	DRWB-013	1	BE32786_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	CTTTAATTACAGCGTTTTTATTGTTGT	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32786_Vgib_Wil2025_COI	DRWB-013	1	BE32786_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	CCCCTCTTTACAAGTTCTTTAGGGT	130.90491
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32787_Asen_Wil2025_COI	DRWB-014	1	BE32787_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	GTTGTTGTTACAACACATGCTCTTTTG	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32787_Cjap_Sim2018_COI	DRWB-014	1	BE32787_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32787_Esin_And2018_Cyb	DRWB-014	1	BE32787_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-014	Cytb	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	ACCCCTCCTCATATCCAACCA	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32787_Mgig_Bot2012_COI	DRWB-014	1	BE32787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	TCTTATTCGTTGGAGACTTTATAACCCT	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32787_Mjap_Gib2022_COI	DRWB-014	1	BE32787_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	AATTTAATTGTGACTAGGCATGGTCTG	130.90613
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32788_Cmae_Bot2011_COI	DRWB-015	1	BE32788_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	ATGAACAGTCTATCCTCCTTTAG	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32788_Hsan_Wil2021_COI	DRWB-015	1	BE32788_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	CCTGGGCCGGTATAGTAGGT	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32788_Mros_Gib2022_COI	DRWB-015	1	BE32788_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32788_Msal_Bot2012_COI	DRWB-015	1	BE32788_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	GYTAGTTCCRATGATGTTAGCTG	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32788_Mstr_Wil2021_COI	DRWB-015	1	BE32788_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	GTAGTGGTAACTACTCATGCTTTA	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32788_Pvir_Dia2013_COI	DRWB-015	1	BE32788_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	CTTAGTGGCATTAATTCGDAATCC	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32788_Rhar_Sim2018_COI	DRWB-015	1	BE32788_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	CTGCTGCTATTGCTCATG	130.90725
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32790_Asen_Wil2025_COI	DRWB-017	1	BE32790_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	GTTGTTGTTACAACACATGCTCTTTTG	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32790_Cjap_Sim2018_COI	DRWB-017	1	BE32790_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32790_Esin_And2018_Cyb	DRWB-017	1	BE32790_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-017	Cytb	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	ACCCCTCCTCATATCCAACCA	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32790_Mgig_Bot2012_COI	DRWB-017	1	BE32790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	TCTTATTCGTTGGAGACTTTATAACCCT	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32790_Mjap_Gib2022_COI	DRWB-017	1	BE32790_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	AATTTAATTGTGACTAGGCATGGTCTG	130.90959
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32791_Cmae_Bot2011_COI	DRWB-018	1	BE32791_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	ATGAACAGTCTATCCTCCTTTAG	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32791_Hsan_Wil2021_COI	DRWB-018	1	BE32791_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	CCTGGGCCGGTATAGTAGGT	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32791_Mros_Gib2022_COI	DRWB-018	1	BE32791_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32791_Msal_Bot2012_COI	DRWB-018	1	BE32791_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	GYTAGTTCCRATGATGTTAGCTG	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32791_Mstr_Wil2021_COI	DRWB-018	1	BE32791_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	GTAGTGGTAACTACTCATGCTTTA	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32791_Pvir_Dia2013_COI	DRWB-018	1	BE32791_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	CTTAGTGGCATTAATTCGDAATCC	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32791_Rhar_Sim2018_COI	DRWB-018	1	BE32791_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	CTGCTGCTATTGCTCATG	130.91082
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32793_Aamu_Bax2006_COI	DRWB-020	1	BE32793_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	GCACAACCGGGATCTTTACTTC	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32793_Pcan_Bot2011_IGS	DRWB-020	1	BE32793_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-020	IGS	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	CGTAATCCTCAGTACTGGCTA	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32793_Pper_Dia2013_COI	DRWB-020	1	BE32793_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	CTTAGTGGCATTAATTCGDAATCC	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32793_Sspa_Oph2007_COI	DRWB-020	1	BE32793_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	GAAGGCCTTCCGATGATC	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32793_Upin_Bot2011_COI	DRWB-020	1	BE32793_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	CTTTAATTACAGCGTTTTTATTGTTGT	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32793_Vgib_Wil2025_COI	DRWB-020	1	BE32793_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	CCCCTCTTTACAAGTTCTTTAGGGT	130.91324
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32794_Asen_Wil2025_COI	DRWB-021	1	BE32794_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	GTTGTTGTTACAACACATGCTCTTTTG	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32794_Cjap_Sim2018_COI	DRWB-021	1	BE32794_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32794_Esin_And2018_Cyb	DRWB-021	1	BE32794_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-021	Cytb	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	ACCCCTCCTCATATCCAACCA	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32794_Mgig_Bot2012_COI	DRWB-021	1	BE32794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	TCTTATTCGTTGGAGACTTTATAACCCT	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32794_Mjap_Gib2022_COI	DRWB-021	1	BE32794_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	AATTTAATTGTGACTAGGCATGGTCTG	130.90562
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32795_Cmae_Bot2011_COI	DRWB-022	1	BE32795_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	ATGAACAGTCTATCCTCCTTTAG	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32795_Hsan_Wil2021_COI	DRWB-022	1	BE32795_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	CCTGGGCCGGTATAGTAGGT	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32795_Mros_Gib2022_COI	DRWB-022	1	BE32795_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32795_Msal_Bot2012_COI	DRWB-022	1	BE32795_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	GYTAGTTCCRATGATGTTAGCTG	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32795_Mstr_Wil2021_COI	DRWB-022	1	BE32795_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	GTAGTGGTAACTACTCATGCTTTA	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32795_Pvir_Dia2013_COI	DRWB-022	1	BE32795_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	CTTAGTGGCATTAATTCGDAATCC	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32795_Rhar_Sim2018_COI	DRWB-022	1	BE32795_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	CTGCTGCTATTGCTCATG	130.90676
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32797_Aamu_Bax2006_COI	DRWB-024	1	BE32797_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	GCACAACCGGGATCTTTACTTC	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32797_Pcan_Bot2011_IGS	DRWB-024	1	BE32797_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-024	IGS	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	CGTAATCCTCAGTACTGGCTA	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32797_Pper_Dia2013_COI	DRWB-024	1	BE32797_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	CTTAGTGGCATTAATTCGDAATCC	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32797_Sspa_Oph2007_COI	DRWB-024	1	BE32797_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	GAAGGCCTTCCGATGATC	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32797_Upin_Bot2011_COI	DRWB-024	1	BE32797_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	CTTTAATTACAGCGTTTTTATTGTTGT	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32797_Vgib_Wil2025_COI	DRWB-024	1	BE32797_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	CCCCTCTTTACAAGTTCTTTAGGGT	130.90914
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32798_Asen_Wil2025_COI	DRWB-025	1	BE32798_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	GTTGTTGTTACAACACATGCTCTTTTG	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32798_Cjap_Sim2018_COI	DRWB-025	1	BE32798_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32798_Esin_And2018_Cyb	DRWB-025	1	BE32798_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-025	Cytb	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	ACCCCTCCTCATATCCAACCA	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32798_Mgig_Bot2012_COI	DRWB-025	1	BE32798_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	TCTTATTCGTTGGAGACTTTATAACCCT	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32798_Mjap_Gib2022_COI	DRWB-025	1	BE32798_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	AATTTAATTGTGACTAGGCATGGTCTG	130.91038
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32799_Cmae_Bot2011_COI	DRWB-026	1	BE32799_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	ATGAACAGTCTATCCTCCTTTAG	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32799_Hsan_Wil2021_COI	DRWB-026	1	BE32799_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	CCTGGGCCGGTATAGTAGGT	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32799_Mros_Gib2022_COI	DRWB-026	1	BE32799_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32799_Msal_Bot2012_COI	DRWB-026	1	BE32799_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	GYTAGTTCCRATGATGTTAGCTG	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32799_Mstr_Wil2021_COI	DRWB-026	1	BE32799_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	GTAGTGGTAACTACTCATGCTTTA	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32799_Pvir_Dia2013_COI	DRWB-026	1	BE32799_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	CTTAGTGGCATTAATTCGDAATCC	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32799_Rhar_Sim2018_COI	DRWB-026	1	BE32799_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	CTGCTGCTATTGCTCATG	130.91155
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32801_Aamu_Bax2006_COI	DRWB-028	1	BE32801_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	GCACAACCGGGATCTTTACTTC	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32801_Pcan_Bot2011_IGS	DRWB-028	1	BE32801_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-028	IGS	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	CGTAATCCTCAGTACTGGCTA	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32801_Pper_Dia2013_COI	DRWB-028	1	BE32801_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	CTTAGTGGCATTAATTCGDAATCC	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32801_Sspa_Oph2007_COI	DRWB-028	1	BE32801_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	GAAGGCCTTCCGATGATC	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32801_Upin_Bot2011_COI	DRWB-028	1	BE32801_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	CTTTAATTACAGCGTTTTTATTGTTGT	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32801_Vgib_Wil2025_COI	DRWB-028	1	BE32801_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	CCCCTCTTTACAAGTTCTTTAGGGT	130.91384
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32802_Asen_Wil2025_COI	DRWB-029	1	BE32802_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	GTTGTTGTTACAACACATGCTCTTTTG	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32802_Cjap_Sim2018_COI	DRWB-029	1	BE32802_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32802_Esin_And2018_Cyb	DRWB-029	1	BE32802_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-029	Cytb	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	ACCCCTCCTCATATCCAACCA	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32802_Mgig_Bot2012_COI	DRWB-029	1	BE32802_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	TCTTATTCGTTGGAGACTTTATAACCCT	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32802_Mjap_Gib2022_COI	DRWB-029	1	BE32802_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	AATTTAATTGTGACTAGGCATGGTCTG	130.91506
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32803_Cmae_Bot2011_COI	DRWB-030	1	BE32803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	ATGAACAGTCTATCCTCCTTTAG	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32803_Hsan_Wil2021_COI	DRWB-030	1	BE32803_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	CCTGGGCCGGTATAGTAGGT	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32803_Mros_Gib2022_COI	DRWB-030	1	BE32803_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32803_Msal_Bot2012_COI	DRWB-030	1	BE32803_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	GYTAGTTCCRATGATGTTAGCTG	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32803_Mstr_Wil2021_COI	DRWB-030	1	BE32803_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	GTAGTGGTAACTACTCATGCTTTA	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32803_Pvir_Dia2013_COI	DRWB-030	1	BE32803_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	CTTAGTGGCATTAATTCGDAATCC	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32803_Rhar_Sim2018_COI	DRWB-030	1	BE32803_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	CTGCTGCTATTGCTCATG	130.90862
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32805_Aamu_Bax2006_COI	DRWB-032	1	BE32805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	GCACAACCGGGATCTTTACTTC	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32805_Pcan_Bot2011_IGS	DRWB-032	1	BE32805_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-032	IGS	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	CGTAATCCTCAGTACTGGCTA	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32805_Pper_Dia2013_COI	DRWB-032	1	BE32805_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	CTTAGTGGCATTAATTCGDAATCC	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32805_Sspa_Oph2007_COI	DRWB-032	1	BE32805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	GAAGGCCTTCCGATGATC	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32805_Upin_Bot2011_COI	DRWB-032	1	BE32805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	CTTTAATTACAGCGTTTTTATTGTTGT	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32805_Vgib_Wil2025_COI	DRWB-032	1	BE32805_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	CCCCTCTTTACAAGTTCTTTAGGGT	130.91099
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32806_Asen_Wil2025_COI	DRWB-033	1	BE32806_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	GTTGTTGTTACAACACATGCTCTTTTG	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32806_Cjap_Sim2018_COI	DRWB-033	1	BE32806_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32806_Esin_And2018_Cyb	DRWB-033	1	BE32806_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-033	Cytb	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	ACCCCTCCTCATATCCAACCA	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32806_Mgig_Bot2012_COI	DRWB-033	1	BE32806_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	TCTTATTCGTTGGAGACTTTATAACCCT	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32806_Mjap_Gib2022_COI	DRWB-033	1	BE32806_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	AATTTAATTGTGACTAGGCATGGTCTG	130.91223
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32807_Cmae_Bot2011_COI	DRWB-034	1	BE32807_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	ATGAACAGTCTATCCTCCTTTAG	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32807_Hsan_Wil2021_COI	DRWB-034	1	BE32807_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	CCTGGGCCGGTATAGTAGGT	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32807_Mros_Gib2022_COI	DRWB-034	1	BE32807_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32807_Msal_Bot2012_COI	DRWB-034	1	BE32807_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	GYTAGTTCCRATGATGTTAGCTG	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32807_Mstr_Wil2021_COI	DRWB-034	1	BE32807_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	GTAGTGGTAACTACTCATGCTTTA	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32807_Pvir_Dia2013_COI	DRWB-034	1	BE32807_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	CTTAGTGGCATTAATTCGDAATCC	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32807_Rhar_Sim2018_COI	DRWB-034	1	BE32807_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	CTGCTGCTATTGCTCATG	130.91346
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32816_Aamu_Bax2006_COI	DRWB-037	1	BE32816_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	GCACAACCGGGATCTTTACTTC	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32816_Pcan_Bot2011_IGS	DRWB-037	1	BE32816_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-037	IGS	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	CGTAATCCTCAGTACTGGCTA	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32816_Pper_Dia2013_COI	DRWB-037	1	BE32816_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	CTTAGTGGCATTAATTCGDAATCC	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32816_Sspa_Oph2007_COI	DRWB-037	1	BE32816_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	GAAGGCCTTCCGATGATC	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32816_Upin_Bot2011_COI	DRWB-037	1	BE32816_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	CTTTAATTACAGCGTTTTTATTGTTGT	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32816_Vgib_Wil2025_COI	DRWB-037	1	BE32816_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	CCCCTCTTTACAAGTTCTTTAGGGT	130.84861
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32817_Asen_Wil2025_COI	DRWB-038	1	BE32817_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	GTTGTTGTTACAACACATGCTCTTTTG	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32817_Cjap_Sim2018_COI	DRWB-038	1	BE32817_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32817_Esin_And2018_Cyb	DRWB-038	1	BE32817_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-038	Cytb	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	ACCCCTCCTCATATCCAACCA	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32817_Mjap_Gib2022_COI	DRWB-038	1	BE32817_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	AATTTAATTGTGACTAGGCATGGTCTG	130.84991
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32818_Cmae_Bot2011_COI	DRWB-039	1	BE32818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	ATGAACAGTCTATCCTCCTTTAG	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32818_Hsan_Wil2021_COI	DRWB-039	1	BE32818_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	CCTGGGCCGGTATAGTAGGT	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32818_Mros_Gib2022_COI	DRWB-039	1	BE32818_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32818_Msal_Bot2012_COI	DRWB-039	1	BE32818_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	GYTAGTTCCRATGATGTTAGCTG	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32818_Mstr_Wil2021_COI	DRWB-039	1	BE32818_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	GTAGTGGTAACTACTCATGCTTTA	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32818_Pvir_Dia2013_COI	DRWB-039	1	BE32818_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	CTTAGTGGCATTAATTCGDAATCC	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32818_Rhar_Sim2018_COI	DRWB-039	1	BE32818_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	CTGCTGCTATTGCTCATG	130.85119
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32821_Cmae_Bot2011_COI	DRWB-042	1	BE32821_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	ATGAACAGTCTATCCTCCTTTAG	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32821_Hsan_Wil2021_COI	DRWB-042	1	BE32821_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	CCTGGGCCGGTATAGTAGGT	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32821_Mros_Gib2022_COI	DRWB-042	1	BE32821_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32821_Msal_Bot2012_COI	DRWB-042	1	BE32821_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	GYTAGTTCCRATGATGTTAGCTG	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32821_Mstr_Wil2021_COI	DRWB-042	1	BE32821_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	GTAGTGGTAACTACTCATGCTTTA	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32821_Pvir_Dia2013_COI	DRWB-042	1	BE32821_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	CTTAGTGGCATTAATTCGDAATCC	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32821_Rhar_Sim2018_COI	DRWB-042	1	BE32821_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	CTGCTGCTATTGCTCATG	130.84791
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32823_Aamu_Bax2006_COI	DRWB-044	1	BE32823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	GCACAACCGGGATCTTTACTTC	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32823_Pcan_Bot2011_IGS	DRWB-044	1	BE32823_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-044	IGS	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	CGTAATCCTCAGTACTGGCTA	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32823_Pper_Dia2013_COI	DRWB-044	1	BE32823_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	CTTAGTGGCATTAATTCGDAATCC	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32823_Sspa_Oph2007_COI	DRWB-044	1	BE32823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	GAAGGCCTTCCGATGATC	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32823_Upin_Bot2011_COI	DRWB-044	1	BE32823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	CTTTAATTACAGCGTTTTTATTGTTGT	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32823_Vgib_Wil2025_COI	DRWB-044	1	BE32823_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	CCCCTCTTTACAAGTTCTTTAGGGT	130.85045
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32824_Asen_Wil2025_COI	DRWB-045	1	BE32824_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	GTTGTTGTTACAACACATGCTCTTTTG	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32824_Cjap_Sim2018_COI	DRWB-045	1	BE32824_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32824_Esin_And2018_Cyb	DRWB-045	1	BE32824_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-045	Cytb	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	ACCCCTCCTCATATCCAACCA	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32824_Mgig_Bot2012_COI	DRWB-045	1	BE32824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	TCTTATTCGTTGGAGACTTTATAACCCT	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32824_Mjap_Gib2022_COI	DRWB-045	1	BE32824_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	AATTTAATTGTGACTAGGCATGGTCTG	130.8519
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32825_Cmae_Bot2011_COI	DRWB-046	1	BE32825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	ATGAACAGTCTATCCTCCTTTAG	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32825_Hsan_Wil2021_COI	DRWB-046	1	BE32825_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	CCTGGGCCGGTATAGTAGGT	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32825_Mros_Gib2022_COI	DRWB-046	1	BE32825_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32825_Msal_Bot2012_COI	DRWB-046	1	BE32825_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	GYTAGTTCCRATGATGTTAGCTG	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32825_Mstr_Wil2021_COI	DRWB-046	1	BE32825_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	GTAGTGGTAACTACTCATGCTTTA	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32825_Pvir_Dia2013_COI	DRWB-046	1	BE32825_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	CTTAGTGGCATTAATTCGDAATCC	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32825_Rhar_Sim2018_COI	DRWB-046	1	BE32825_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	CTGCTGCTATTGCTCATG	130.85325
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32827_Aamu_Bax2006_COI	DRWB-048	1	BE32827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	GCACAACCGGGATCTTTACTTC	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32827_Pcan_Bot2011_IGS	DRWB-048	1	BE32827_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-048	IGS	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	CGTAATCCTCAGTACTGGCTA	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32827_Pper_Dia2013_COI	DRWB-048	1	BE32827_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	CTTAGTGGCATTAATTCGDAATCC	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32827_Sspa_Oph2007_COI	DRWB-048	1	BE32827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	GAAGGCCTTCCGATGATC	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32827_Upin_Bot2011_COI	DRWB-048	1	BE32827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	CTTTAATTACAGCGTTTTTATTGTTGT	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32827_Vgib_Wil2025_COI	DRWB-048	1	BE32827_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	CCCCTCTTTACAAGTTCTTTAGGGT	130.8473
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32828_Asen_Wil2025_COI	DRWB-049	1	BE32828_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	GTTGTTGTTACAACACATGCTCTTTTG	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32828_Cjap_Sim2018_COI	DRWB-049	1	BE32828_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32828_Esin_And2018_Cyb	DRWB-049	1	BE32828_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-049	Cytb	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	ACCCCTCCTCATATCCAACCA	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32828_Mgig_Bot2012_COI	DRWB-049	1	BE32828_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	TCTTATTCGTTGGAGACTTTATAACCCT	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32828_Mjap_Gib2022_COI	DRWB-049	1	BE32828_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	AATTTAATTGTGACTAGGCATGGTCTG	130.84536
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32829_Cmae_Bot2011_COI	DRWB-050	1	BE32829_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	ATGAACAGTCTATCCTCCTTTAG	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32829_Hsan_Wil2021_COI	DRWB-050	1	BE32829_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	CCTGGGCCGGTATAGTAGGT	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32829_Mros_Gib2022_COI	DRWB-050	1	BE32829_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32829_Msal_Bot2012_COI	DRWB-050	1	BE32829_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	GYTAGTTCCRATGATGTTAGCTG	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32829_Mstr_Wil2021_COI	DRWB-050	1	BE32829_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	GTAGTGGTAACTACTCATGCTTTA	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32829_Pvir_Dia2013_COI	DRWB-050	1	BE32829_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	CTTAGTGGCATTAATTCGDAATCC	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32829_Rhar_Sim2018_COI	DRWB-050	1	BE32829_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	CTGCTGCTATTGCTCATG	130.84662
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32830_Aamu_Bax2006_COI	DRWB-051	1	BE32830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	GCACAACCGGGATCTTTACTTC	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32830_Pcan_Bot2011_IGS	DRWB-051	1	BE32830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-051	IGS	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	CGTAATCCTCAGTACTGGCTA	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32830_Pper_Dia2013_COI	DRWB-051	1	BE32830_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	CTTAGTGGCATTAATTCGDAATCC	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32830_Sspa_Oph2007_COI	DRWB-051	1	BE32830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	GAAGGCCTTCCGATGATC	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32830_Upin_Bot2011_COI	DRWB-051	1	BE32830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	CTTTAATTACAGCGTTTTTATTGTTGT	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32830_Vgib_Wil2025_COI	DRWB-051	1	BE32830_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	CCCCTCTTTACAAGTTCTTTAGGGT	130.848
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32831_Asen_Wil2025_COI	DRWB-052	1	BE32831_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	GTTGTTGTTACAACACATGCTCTTTTG	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32831_Cjap_Sim2018_COI	DRWB-052	1	BE32831_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32831_Esin_And2018_Cyb	DRWB-052	1	BE32831_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-052	Cytb	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	ACCCCTCCTCATATCCAACCA	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32831_Mgig_Bot2012_COI	DRWB-052	1	BE32831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	TCTTATTCGTTGGAGACTTTATAACCCT	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32831_Mjap_Gib2022_COI	DRWB-052	1	BE32831_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	AATTTAATTGTGACTAGGCATGGTCTG	130.85133
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32832_Cmae_Bot2011_COI	DRWB-053	1	BE32832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	ATGAACAGTCTATCCTCCTTTAG	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32832_Hsan_Wil2021_COI	DRWB-053	1	BE32832_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	CCTGGGCCGGTATAGTAGGT	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32832_Mros_Gib2022_COI	DRWB-053	1	BE32832_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32832_Msal_Bot2012_COI	DRWB-053	1	BE32832_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	GYTAGTTCCRATGATGTTAGCTG	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32832_Mstr_Wil2021_COI	DRWB-053	1	BE32832_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	GTAGTGGTAACTACTCATGCTTTA	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32832_Pvir_Dia2013_COI	DRWB-053	1	BE32832_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	CTTAGTGGCATTAATTCGDAATCC	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32832_Rhar_Sim2018_COI	DRWB-053	1	BE32832_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	CTGCTGCTATTGCTCATG	130.84465
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32834_Aamu_Bax2006_COI	DRWB-055	1	BE32834_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	GCACAACCGGGATCTTTACTTC	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32834_Pcan_Bot2011_IGS	DRWB-055	1	BE32834_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-055	IGS	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	CGTAATCCTCAGTACTGGCTA	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32834_Pper_Dia2013_COI	DRWB-055	1	BE32834_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	CTTAGTGGCATTAATTCGDAATCC	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32834_Sspa_Oph2007_COI	DRWB-055	1	BE32834_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	GAAGGCCTTCCGATGATC	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32834_Upin_Bot2011_COI	DRWB-055	1	BE32834_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	CTTTAATTACAGCGTTTTTATTGTTGT	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32834_Vgib_Wil2025_COI	DRWB-055	1	BE32834_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	CCCCTCTTTACAAGTTCTTTAGGGT	130.84732
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32835_Asen_Wil2025_COI	DRWB-056	1	BE32835_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	GTTGTTGTTACAACACATGCTCTTTTG	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32835_Cjap_Sim2018_COI	DRWB-056	1	BE32835_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32835_Esin_And2018_Cyb	DRWB-056	1	BE32835_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-056	Cytb	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	ACCCCTCCTCATATCCAACCA	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32835_Mgig_Bot2012_COI	DRWB-056	1	BE32835_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	TCTTATTCGTTGGAGACTTTATAACCCT	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32835_Mjap_Gib2022_COI	DRWB-056	1	BE32835_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	AATTTAATTGTGACTAGGCATGGTCTG	130.84535
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32836_Cmae_Bot2011_COI	DRWB-057	1	BE32836_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	ATGAACAGTCTATCCTCCTTTAG	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32836_Hsan_Wil2021_COI	DRWB-057	1	BE32836_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	CCTGGGCCGGTATAGTAGGT	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32836_Mros_Gib2022_COI	DRWB-057	1	BE32836_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32836_Msal_Bot2012_COI	DRWB-057	1	BE32836_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	GYTAGTTCCRATGATGTTAGCTG	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32836_Mstr_Wil2021_COI	DRWB-057	1	BE32836_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	GTAGTGGTAACTACTCATGCTTTA	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32836_Pvir_Dia2013_COI	DRWB-057	1	BE32836_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	CTTAGTGGCATTAATTCGDAATCC	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32836_Rhar_Sim2018_COI	DRWB-057	1	BE32836_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	CTGCTGCTATTGCTCATG	130.84667
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32838_Aamu_Bax2006_COI	DRWB-059	1	BE32838_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	GCACAACCGGGATCTTTACTTC	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32838_Pcan_Bot2011_IGS	DRWB-059	1	BE32838_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-059	IGS	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	CGTAATCCTCAGTACTGGCTA	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32838_Pper_Dia2013_COI	DRWB-059	1	BE32838_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	CTTAGTGGCATTAATTCGDAATCC	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32838_Sspa_Oph2007_COI	DRWB-059	1	BE32838_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	GAAGGCCTTCCGATGATC	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32838_Upin_Bot2011_COI	DRWB-059	1	BE32838_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	CTTTAATTACAGCGTTTTTATTGTTGT	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32838_Vgib_Wil2025_COI	DRWB-059	1	BE32838_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	CCCCTCTTTACAAGTTCTTTAGGGT	130.84464
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32839_Asen_Wil2025_COI	DRWB-060	1	BE32839_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	GTTGTTGTTACAACACATGCTCTTTTG	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32839_Cjap_Sim2018_COI	DRWB-060	1	BE32839_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32839_Esin_And2018_Cyb	DRWB-060	1	BE32839_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-060	Cytb	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	ACCCCTCCTCATATCCAACCA	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32839_Mgig_Bot2012_COI	DRWB-060	1	BE32839_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	TCTTATTCGTTGGAGACTTTATAACCCT	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32839_Mjap_Gib2022_COI	DRWB-060	1	BE32839_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	AATTTAATTGTGACTAGGCATGGTCTG	130.84601
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32841_Aamu_Bax2006_COI	DRWB-062	1	BE32841_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	GCACAACCGGGATCTTTACTTC	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32841_Pcan_Bot2011_IGS	DRWB-062	1	BE32841_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-062	IGS	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	CGTAATCCTCAGTACTGGCTA	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32841_Pper_Dia2013_COI	DRWB-062	1	BE32841_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	CTTAGTGGCATTAATTCGDAATCC	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32841_Sspa_Oph2007_COI	DRWB-062	1	BE32841_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	GAAGGCCTTCCGATGATC	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32841_Upin_Bot2011_COI	DRWB-062	1	BE32841_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	CTTTAATTACAGCGTTTTTATTGTTGT	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32841_Vgib_Wil2025_COI	DRWB-062	1	BE32841_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	CCCCTCTTTACAAGTTCTTTAGGGT	130.84871
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32842_Asen_Wil2025_COI	DRWB-063	1	BE32842_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	GTTGTTGTTACAACACATGCTCTTTTG	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32842_Cjap_Sim2018_COI	DRWB-063	1	BE32842_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32842_Esin_And2018_Cyb	DRWB-063	1	BE32842_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-063	Cytb	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	ACCCCTCCTCATATCCAACCA	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32842_Mgig_Bot2012_COI	DRWB-063	1	BE32842_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	TCTTATTCGTTGGAGACTTTATAACCCT	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32842_Mjap_Gib2022_COI	DRWB-063	1	BE32842_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	AATTTAATTGTGACTAGGCATGGTCTG	130.85008
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32843_Cmae_Bot2011_COI	DRWB-064	1	BE32843_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	ATGAACAGTCTATCCTCCTTTAG	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32843_Hsan_Wil2021_COI	DRWB-064	1	BE32843_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	CCTGGGCCGGTATAGTAGGT	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32843_Mros_Gib2022_COI	DRWB-064	1	BE32843_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32843_Msal_Bot2012_COI	DRWB-064	1	BE32843_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	GYTAGTTCCRATGATGTTAGCTG	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32843_Mstr_Wil2021_COI	DRWB-064	1	BE32843_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	GTAGTGGTAACTACTCATGCTTTA	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32843_Pvir_Dia2013_COI	DRWB-064	1	BE32843_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	CTTAGTGGCATTAATTCGDAATCC	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32843_Rhar_Sim2018_COI	DRWB-064	1	BE32843_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	CTGCTGCTATTGCTCATG	130.84544
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32845_Aamu_Bax2006_COI	DRWB-066	1	BE32845_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	GCACAACCGGGATCTTTACTTC	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32845_Pcan_Bot2011_IGS	DRWB-066	1	BE32845_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-066	IGS	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	CGTAATCCTCAGTACTGGCTA	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32845_Pper_Dia2013_COI	DRWB-066	1	BE32845_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	CTTAGTGGCATTAATTCGDAATCC	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32845_Sspa_Oph2007_COI	DRWB-066	1	BE32845_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	GAAGGCCTTCCGATGATC	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32845_Upin_Bot2011_COI	DRWB-066	1	BE32845_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	CTTTAATTACAGCGTTTTTATTGTTGT	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32845_Vgib_Wil2025_COI	DRWB-066	1	BE32845_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	CCCCTCTTTACAAGTTCTTTAGGGT	130.8481
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32846_Asen_Wil2025_COI	DRWB-067	1	BE32846_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	GTTGTTGTTACAACACATGCTCTTTTG	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32846_Cjap_Sim2018_COI	DRWB-067	1	BE32846_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32846_Esin_And2018_Cyb	DRWB-067	1	BE32846_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-067	Cytb	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	ACCCCTCCTCATATCCAACCA	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32846_Mgig_Bot2012_COI	DRWB-067	1	BE32846_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	TCTTATTCGTTGGAGACTTTATAACCCT	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32846_Mjap_Gib2022_COI	DRWB-067	1	BE32846_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	AATTTAATTGTGACTAGGCATGGTCTG	130.84942
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32847_Cmae_Bot2011_COI	DRWB-068	1	BE32847_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	ATGAACAGTCTATCCTCCTTTAG	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32847_Hsan_Wil2021_COI	DRWB-068	1	BE32847_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	CCTGGGCCGGTATAGTAGGT	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32847_Mros_Gib2022_COI	DRWB-068	1	BE32847_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32847_Msal_Bot2012_COI	DRWB-068	1	BE32847_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	GYTAGTTCCRATGATGTTAGCTG	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32847_Mstr_Wil2021_COI	DRWB-068	1	BE32847_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	GTAGTGGTAACTACTCATGCTTTA	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32847_Pvir_Dia2013_COI	DRWB-068	1	BE32847_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	CTTAGTGGCATTAATTCGDAATCC	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32847_Rhar_Sim2018_COI	DRWB-068	1	BE32847_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	CTGCTGCTATTGCTCATG	130.84744
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32849_Aamu_Bax2006_COI	DRWB-070	1	BE32849_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	GCACAACCGGGATCTTTACTTC	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32849_Pcan_Bot2011_IGS	DRWB-070	1	BE32849_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-070	IGS	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	CGTAATCCTCAGTACTGGCTA	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32849_Pper_Dia2013_COI	DRWB-070	1	BE32849_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	CTTAGTGGCATTAATTCGDAATCC	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32849_Sspa_Oph2007_COI	DRWB-070	1	BE32849_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	GAAGGCCTTCCGATGATC	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32849_Upin_Bot2011_COI	DRWB-070	1	BE32849_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	CTTTAATTACAGCGTTTTTATTGTTGT	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32849_Vgib_Wil2025_COI	DRWB-070	1	BE32849_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	CCCCTCTTTACAAGTTCTTTAGGGT	130.85013
id	detectionCriteria	pcrPlateID	decimalLatitude	geodeticDatum	materialSampleID	pcrProbeSeq	pcrProbeRepoter	ampVisMethod	basisOfRecord	quantificationCycle	coordinateUncertaintyInMetres	targetTaxonomicAssay	sampleMaterialProcess	pcrInhibitionScaleFactor	pcrProbeQuencher	associatedReferences	parentEventID	eventDate	pcrPrimerREV	biome	scientificName	pcrEfficiency	pcrAmpliconSize	catalogueNumber	sourceMaterialID	pcrInhibitionCheck01	occurrenceID	samplingProtocol	sampleCollectionMethod	eventID	eventTypeHierarchy	sampleName	assayType	occurrenceStatus	eventType	assayName	materialEntityID	pcrTargetGene	pcrCycles	eventHierarchy	coordinatePrecision	pcrInhibitionCheck	country	fieldNumber	pcrPrimerFWD	decimalLongitude
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83962_Cmae_Bot2011_COI	C4	1	BA83962_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Cmae_Bot2011_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	ATGAACAGTCTATCCTCCTTTAG	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83962_Hsan_Wil2021_COI	C4	1	BA83962_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Hsan_Wil2021_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	CCTGGGCCGGTATAGTAGGT	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83962_Mros_Gib2022_COI	C4	1	BA83962_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Mros_Gib2022_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83962_Msal_Bot2012_COI	C4	1	BA83962_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Msal_Bot2012_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	GYTAGTTCCRATGATGTTAGCTG	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83962_Pvir_Dia2013_COI	C4	1	BA83962_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Pvir_Dia2013_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	CTTAGTGGCATTAATTCGDAATCC	145.77379
BA83962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95175	WGS84	BA83962	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83962_Rhar_Sim2018_COI	C4	1	BA83962_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83962	Sample	BA83962	targeted	Absent	Sample	Rhar_Sim2018_COI	C4	COI	45	BA83962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C4	CTGCTGCTATTGCTCATG	145.77379
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83964_Aamu_Bax2006_COI	C6	1	BA83964_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Aamu_Bax2006_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	GCACAACCGGGATCTTTACTTC	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83964_Pcan_Bot2011_IGS	C6	1	BA83964_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Pcan_Bot2011_IGS	C6	IGS	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	CGTAATCCTCAGTACTGGCTA	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83964_Pper_Dia2013_COI	C6	1	BA83964_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Pper_Dia2013_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	CTTAGTGGCATTAATTCGDAATCC	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83964_Sspa_Oph2007_COI	C6	1	BA83964_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Sspa_Oph2007_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	GAAGGCCTTCCGATGATC	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83964_Upin_Bot2011_COI	C6	1	BA83964_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Upin_Bot2011_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	CTTTAATTACAGCGTTTTTATTGTTGT	145.7847
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83965_Cjap_Sim2018_COI	C10	1	BA83965_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Cjap_Sim2018_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83965_Esin_And2018_Cyb	C10	1	BA83965_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Esin_And2018_Cyb	C10	Cytb	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	ACCCCTCCTCATATCCAACCA	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83965_Mgig_Bot2012_COI	C10	1	BA83965_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Mgig_Bot2012_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	TCTTATTCGTTGGAGACTTTATAACCCT	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA83965_Mjap_Gib2022_COI	C10	1	BA83965_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Mjap_Gib2022_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	AATTTAATTGTGACTAGGCATGGTCTG	145.78334
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83966_Cmae_Bot2011_COI	C11	1	BA83966_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Cmae_Bot2011_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	ATGAACAGTCTATCCTCCTTTAG	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83966_Hsan_Wil2021_COI	C11	1	BA83966_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Hsan_Wil2021_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	CCTGGGCCGGTATAGTAGGT	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83966_Mros_Gib2022_COI	C11	1	BA83966_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Mros_Gib2022_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83966_Msal_Bot2012_COI	C11	1	BA83966_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Msal_Bot2012_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	GYTAGTTCCRATGATGTTAGCTG	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83966_Pvir_Dia2013_COI	C11	1	BA83966_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Pvir_Dia2013_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	CTTAGTGGCATTAATTCGDAATCC	145.7845
BA83966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83966	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83966_Rhar_Sim2018_COI	C11	1	BA83966_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83966	Sample	BA83966	targeted	Absent	Sample	Rhar_Sim2018_COI	C11	COI	45	BA83966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C11	CTGCTGCTATTGCTCATG	145.7845
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83968_Aamu_Bax2006_COI	C16	1	BA83968_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Aamu_Bax2006_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	GCACAACCGGGATCTTTACTTC	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83968_Pcan_Bot2011_IGS	C16	1	BA83968_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Pcan_Bot2011_IGS	C16	IGS	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	CGTAATCCTCAGTACTGGCTA	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83968_Pper_Dia2013_COI	C16	1	BA83968_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Pper_Dia2013_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	CTTAGTGGCATTAATTCGDAATCC	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83968_Sspa_Oph2007_COI	C16	1	BA83968_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Sspa_Oph2007_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	GAAGGCCTTCCGATGATC	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83968_Upin_Bot2011_COI	C16	1	BA83968_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Upin_Bot2011_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	CTTTAATTACAGCGTTTTTATTGTTGT	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83969_Cjap_Sim2018_COI	C17	1	BA83969_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Cjap_Sim2018_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83969_Esin_And2018_Cyb	C17	1	BA83969_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Esin_And2018_Cyb	C17	Cytb	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	ACCCCTCCTCATATCCAACCA	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83969_Mgig_Bot2012_COI	C17	1	BA83969_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Mgig_Bot2012_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	TCTTATTCGTTGGAGACTTTATAACCCT	145.78604
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83971_Aamu_Bax2006_COI	C22	1	BA83971_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Aamu_Bax2006_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	GCACAACCGGGATCTTTACTTC	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83971_Pcan_Bot2011_IGS	C22	1	BA83971_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Pcan_Bot2011_IGS	C22	IGS	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	CGTAATCCTCAGTACTGGCTA	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83971_Pper_Dia2013_COI	C22	1	BA83971_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Pper_Dia2013_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	CTTAGTGGCATTAATTCGDAATCC	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83971_Sspa_Oph2007_COI	C22	1	BA83971_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Sspa_Oph2007_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	GAAGGCCTTCCGATGATC	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83971_Upin_Bot2011_COI	C22	1	BA83971_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Upin_Bot2011_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	CTTTAATTACAGCGTTTTTATTGTTGT	145.78376
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83972_Cjap_Sim2018_COI	C23	1	BA83972_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Cjap_Sim2018_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83972_Esin_And2018_Cyb	C23	1	BA83972_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Esin_And2018_Cyb	C23	Cytb	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	ACCCCTCCTCATATCCAACCA	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83972_Mgig_Bot2012_COI	C23	1	BA83972_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Mgig_Bot2012_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	TCTTATTCGTTGGAGACTTTATAACCCT	145.78649
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83973_Cmae_Bot2011_COI	C24	1	BA83973_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Cmae_Bot2011_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	ATGAACAGTCTATCCTCCTTTAG	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83973_Hsan_Wil2021_COI	C24	1	BA83973_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Hsan_Wil2021_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	CCTGGGCCGGTATAGTAGGT	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83973_Mros_Gib2022_COI	C24	1	BA83973_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Mros_Gib2022_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83973_Msal_Bot2012_COI	C24	1	BA83973_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Msal_Bot2012_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	GYTAGTTCCRATGATGTTAGCTG	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83973_Pvir_Dia2013_COI	C24	1	BA83973_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Pvir_Dia2013_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	CTTAGTGGCATTAATTCGDAATCC	145.78334
BA83973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83973	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83973_Rhar_Sim2018_COI	C24	1	BA83973_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83973	Sample	BA83973	targeted	Absent	Sample	Rhar_Sim2018_COI	C24	COI	45	BA83973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C24	CTGCTGCTATTGCTCATG	145.78334
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83975_Aamu_Bax2006_COI	C29	1	BA83975_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Aamu_Bax2006_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	GCACAACCGGGATCTTTACTTC	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83975_Pcan_Bot2011_IGS	C29	1	BA83975_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Pcan_Bot2011_IGS	C29	IGS	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	CGTAATCCTCAGTACTGGCTA	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83975_Pper_Dia2013_COI	C29	1	BA83975_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Pper_Dia2013_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	CTTAGTGGCATTAATTCGDAATCC	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83975_Sspa_Oph2007_COI	C29	1	BA83975_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Sspa_Oph2007_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	GAAGGCCTTCCGATGATC	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83975_Upin_Bot2011_COI	C29	1	BA83975_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Upin_Bot2011_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	CTTTAATTACAGCGTTTTTATTGTTGT	145.78312
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83976_Cjap_Sim2018_COI	C30	1	BA83976_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Cjap_Sim2018_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83976_Esin_And2018_Cyb	C30	1	BA83976_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Esin_And2018_Cyb	C30	Cytb	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	ACCCCTCCTCATATCCAACCA	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83976_Mgig_Bot2012_COI	C30	1	BA83976_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Mgig_Bot2012_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	TCTTATTCGTTGGAGACTTTATAACCCT	145.7845
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83977_Cmae_Bot2011_COI	A85	1	BA83977_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Cmae_Bot2011_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	ATGAACAGTCTATCCTCCTTTAG	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83977_Hsan_Wil2021_COI	A85	1	BA83977_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Hsan_Wil2021_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	CCTGGGCCGGTATAGTAGGT	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83977_Mros_Gib2022_COI	A85	1	BA83977_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Mros_Gib2022_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83977_Msal_Bot2012_COI	A85	1	BA83977_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Msal_Bot2012_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	GYTAGTTCCRATGATGTTAGCTG	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83977_Pvir_Dia2013_COI	A85	1	BA83977_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Pvir_Dia2013_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	CTTAGTGGCATTAATTCGDAATCC	138.52559
BA83977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81109	WGS84	BA83977	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83977_Rhar_Sim2018_COI	A85	1	BA83977_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83977	Sample	BA83977	targeted	Absent	Sample	Rhar_Sim2018_COI	A85	COI	45	BA83977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A85	CTGCTGCTATTGCTCATG	138.52559
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83979_Aamu_Bax2006_COI	A87	1	BA83979_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Aamu_Bax2006_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	GCACAACCGGGATCTTTACTTC	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83979_Pcan_Bot2011_IGS	A87	1	BA83979_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Pcan_Bot2011_IGS	A87	IGS	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	CGTAATCCTCAGTACTGGCTA	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83979_Pper_Dia2013_COI	A87	1	BA83979_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Pper_Dia2013_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	CTTAGTGGCATTAATTCGDAATCC	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83979_Sspa_Oph2007_COI	A87	1	BA83979_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Sspa_Oph2007_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	GAAGGCCTTCCGATGATC	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83979_Upin_Bot2011_COI	A87	1	BA83979_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Upin_Bot2011_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	CTTTAATTACAGCGTTTTTATTGTTGT	138.51686
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83980_Cmae_Bot2011_COI	A88	1	BA83980_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Cmae_Bot2011_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	ATGAACAGTCTATCCTCCTTTAG	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83980_Hsan_Wil2021_COI	A88	1	BA83980_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Hsan_Wil2021_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	CCTGGGCCGGTATAGTAGGT	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83980_Mros_Gib2022_COI	A88	1	BA83980_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Mros_Gib2022_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83980_Msal_Bot2012_COI	A88	1	BA83980_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Msal_Bot2012_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	GYTAGTTCCRATGATGTTAGCTG	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83980_Pvir_Dia2013_COI	A88	1	BA83980_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Pvir_Dia2013_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	CTTAGTGGCATTAATTCGDAATCC	138.51964
BA83980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80985	WGS84	BA83980	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83980_Rhar_Sim2018_COI	A88	1	BA83980_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83980	Sample	BA83980	targeted	Absent	Sample	Rhar_Sim2018_COI	A88	COI	45	BA83980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A88	CTGCTGCTATTGCTCATG	138.51964
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83982_Aamu_Bax2006_COI	A90	1	BA83982_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Aamu_Bax2006_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	GCACAACCGGGATCTTTACTTC	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83982_Pcan_Bot2011_IGS	A90	1	BA83982_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Pcan_Bot2011_IGS	A90	IGS	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	CGTAATCCTCAGTACTGGCTA	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83982_Pper_Dia2013_COI	A90	1	BA83982_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Pper_Dia2013_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	CTTAGTGGCATTAATTCGDAATCC	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83982_Sspa_Oph2007_COI	A90	1	BA83982_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Sspa_Oph2007_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	GAAGGCCTTCCGATGATC	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83982_Upin_Bot2011_COI	A90	1	BA83982_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Upin_Bot2011_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	CTTTAATTACAGCGTTTTTATTGTTGT	138.51743
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83983_Cjap_Sim2018_COI	A91	1	BA83983_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Cjap_Sim2018_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83983_Esin_And2018_Cyb	A91	1	BA83983_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Esin_And2018_Cyb	A91	Cytb	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	ACCCCTCCTCATATCCAACCA	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83983_Mgig_Bot2012_COI	A91	1	BA83983_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Mgig_Bot2012_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	TCTTATTCGTTGGAGACTTTATAACCCT	138.52058
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83984_Cmae_Bot2011_COI	A92	1	BA83984_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Cmae_Bot2011_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	ATGAACAGTCTATCCTCCTTTAG	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83984_Hsan_Wil2021_COI	A92	1	BA83984_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Hsan_Wil2021_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	CCTGGGCCGGTATAGTAGGT	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83984_Mros_Gib2022_COI	A92	1	BA83984_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Mros_Gib2022_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83984_Msal_Bot2012_COI	A92	1	BA83984_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Msal_Bot2012_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	GYTAGTTCCRATGATGTTAGCTG	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83984_Pvir_Dia2013_COI	A92	1	BA83984_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Pvir_Dia2013_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	CTTAGTGGCATTAATTCGDAATCC	138.51769
BA83984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80885	WGS84	BA83984	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83984_Rhar_Sim2018_COI	A92	1	BA83984_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83984	Sample	BA83984	targeted	Absent	Sample	Rhar_Sim2018_COI	A92	COI	45	BA83984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A92	CTGCTGCTATTGCTCATG	138.51769
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83986_Aamu_Bax2006_COI	A94	1	BA83986_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Aamu_Bax2006_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	GCACAACCGGGATCTTTACTTC	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83986_Pcan_Bot2011_IGS	A94	1	BA83986_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Pcan_Bot2011_IGS	A94	IGS	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	CGTAATCCTCAGTACTGGCTA	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83986_Pper_Dia2013_COI	A94	1	BA83986_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Pper_Dia2013_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	CTTAGTGGCATTAATTCGDAATCC	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83986_Sspa_Oph2007_COI	A94	1	BA83986_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Sspa_Oph2007_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	GAAGGCCTTCCGATGATC	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83986_Upin_Bot2011_COI	A94	1	BA83986_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Upin_Bot2011_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	CTTTAATTACAGCGTTTTTATTGTTGT	138.52268
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83987_Cjap_Sim2018_COI	A95	1	BA83987_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Cjap_Sim2018_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83987_Esin_And2018_Cyb	A95	1	BA83987_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Esin_And2018_Cyb	A95	Cytb	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	ACCCCTCCTCATATCCAACCA	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83987_Mgig_Bot2012_COI	A95	1	BA83987_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Mgig_Bot2012_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	TCTTATTCGTTGGAGACTTTATAACCCT	138.52086
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.861	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83988_Cmae_Bot2011_COI	A96	1	BA83988_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Present	Sample	Cmae_Bot2011_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	ATGAACAGTCTATCCTCCTTTAG	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83988_Hsan_Wil2021_COI	A96	1	BA83988_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Hsan_Wil2021_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	CCTGGGCCGGTATAGTAGGT	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83988_Mros_Gib2022_COI	A96	1	BA83988_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Mros_Gib2022_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83988_Msal_Bot2012_COI	A96	1	BA83988_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Msal_Bot2012_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	GYTAGTTCCRATGATGTTAGCTG	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83988_Pvir_Dia2013_COI	A96	1	BA83988_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Pvir_Dia2013_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	CTTAGTGGCATTAATTCGDAATCC	138.51953
BA83988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80949	WGS84	BA83988	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83988_Rhar_Sim2018_COI	A96	1	BA83988_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83988	Sample	BA83988	targeted	Absent	Sample	Rhar_Sim2018_COI	A96	COI	45	BA83988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A96	CTGCTGCTATTGCTCATG	138.51953
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA87564_Pper_Dia2013_COI	D5	1	BA87564_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Pper_Dia2013_COI	D5	COI	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	CTTAGTGGCATTAATTCGDAATCC	130.85486
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA87565_Cjap_Sim2018_COI	D6	1	BA87565_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Cjap_Sim2018_COI	D6	COI	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87387
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA87565_Esin_And2018_Cyb	D6	1	BA87565_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Esin_And2018_Cyb	D6	Cytb	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	ACCCCTCCTCATATCCAACCA	130.87387
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA87565_Mjap_Gib2022_COI	D6	1	BA87565_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Mjap_Gib2022_COI	D6	COI	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	AATTTAATTGTGACTAGGCATGGTCTG	130.87387
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA87566_Hsan_Wil2021_COI	D10	1	BA87566_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Hsan_Wil2021_COI	D10	COI	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	CCTGGGCCGGTATAGTAGGT	130.87402
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA87566_Mros_Gib2022_COI	D10	1	BA87566_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Mros_Gib2022_COI	D10	COI	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87402
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA87566_Pvir_Dia2013_COI	D10	1	BA87566_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Pvir_Dia2013_COI	D10	COI	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	CTTAGTGGCATTAATTCGDAATCC	130.87402
BA87566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.49014	WGS84	BA87566	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA87566_Rhar_Sim2018_COI	D10	1	BA87566_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87566	Sample	BA87566	targeted	Absent	Sample	Rhar_Sim2018_COI	D10	COI	45	BA87566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D10	CTGCTGCTATTGCTCATG	130.87402
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA87568_Pper_Dia2013_COI	D12	1	BA87568_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Pper_Dia2013_COI	D12	COI	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	CTTAGTGGCATTAATTCGDAATCC	130.8249
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA87569_Cjap_Sim2018_COI	D22	1	BA87569_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Cjap_Sim2018_COI	D22	COI	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.82694
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA87569_Esin_And2018_Cyb	D22	1	BA87569_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Esin_And2018_Cyb	D22	Cytb	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	ACCCCTCCTCATATCCAACCA	130.82694
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA87569_Mjap_Gib2022_COI	D22	1	BA87569_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Mjap_Gib2022_COI	D22	COI	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	AATTTAATTGTGACTAGGCATGGTCTG	130.82694
BA92893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-42.88391	WGS84	BA92893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.922	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_1	29/05/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA92893_Mgig_Bot2012_COI	H17	1	BA92893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92893	Sample	BA92893	targeted	Present	Sample	Mgig_Bot2012_COI	H17	COI	45	BA92893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H17	TCTTATTCGTTGGAGACTTTATAACCCT	147.33791
BA92908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77803	WGS84	BA92908	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.51481628	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92908_Sspa_Oph2007_COI	NP14	1	BA92908_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92908	Sample	BA92908	targeted	Present	Sample	Sspa_Oph2007_COI	NP14	COI	45	BA92908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP14	GAAGGCCTTCCGATGATC	138.48172
BA92911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77537	WGS84	BA92911	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.99704361	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92911_Sspa_Oph2007_COI	NP13	1	BA92911_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92911	Sample	BA92911	targeted	Present	Sample	Sspa_Oph2007_COI	NP13	COI	45	BA92911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP13	GAAGGCCTTCCGATGATC	138.48363
BA92919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77593	WGS84	BA92919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.5731926	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92919_Sspa_Oph2007_COI	NP5	1	BA92919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92919	Sample	BA92919	targeted	Present	Sample	Sspa_Oph2007_COI	NP5	COI	45	BA92919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP5	GAAGGCCTTCCGATGATC	138.48309
BA92935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77575	WGS84	BA92935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.658	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92935_Cmae_Bot2011_COI	NP7	1	BA92935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92935	Sample	BA92935	targeted	Present	Sample	Cmae_Bot2011_COI	NP7	COI	45	BA92935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP7	ATGAACAGTCTATCCTCCTTTAG	138.48302
BA92942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77755	WGS84	BA92942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.657	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92942_Cmae_Bot2011_COI	NP16	1	BA92942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92942	Sample	BA92942	targeted	Present	Sample	Cmae_Bot2011_COI	NP16	COI	45	BA92942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP16	ATGAACAGTCTATCCTCCTTTAG	138.48188
BA92948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77739	WGS84	BA92948	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.50551605	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92948_Sspa_Oph2007_COI	NP18	1	BA92948_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92948	Sample	BA92948	targeted	Present	Sample	Sspa_Oph2007_COI	NP18	COI	45	BA92948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP18	GAAGGCCTTCCGATGATC	138.48206
BA99033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.82031	WGS84	BA99033	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.522	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99033_Aamu_Bax2006_COI	M30	1	BA99033_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99033	Sample	BA99033	targeted	Present	Sample	Aamu_Bax2006_COI	M30	COI	45	BA99033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M30	GCACAACCGGGATCTTTACTTC	144.90882
BA99035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84518	WGS84	BA99035	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.632	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA99035_Cmae_Bot2011_COI	M5	1	BA99035_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99035	Sample	BA99035	targeted	Present	Sample	Cmae_Bot2011_COI	M5	COI	45	BA99035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M5	ATGAACAGTCTATCCTCCTTTAG	144.9296
BA99037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84242	WGS84	BA99037	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.713	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99037_Aamu_Bax2006_COI	M10	1	BA99037_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99037	Sample	BA99037	targeted	Present	Sample	Aamu_Bax2006_COI	M10	COI	45	BA99037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M10	GCACAACCGGGATCTTTACTTC	144.92592
BA99037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84242	WGS84	BA99037	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.111	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA99037_Upin_Bot2011_COI	M10	1	BA99037_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99037	Sample	BA99037	targeted	Present	Sample	Upin_Bot2011_COI	M10	COI	45	BA99037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M10	CTTTAATTACAGCGTTTTTATTGTTGT	144.92592
BA99040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84124	WGS84	BA99040	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.779	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99040_Aamu_Bax2006_COI	M22	1	BA99040_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99040	Sample	BA99040	targeted	Present	Sample	Aamu_Bax2006_COI	M22	COI	45	BA99040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M22	GCACAACCGGGATCTTTACTTC	144.92811
BA99040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84124	WGS84	BA99040	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.146	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA99040_Upin_Bot2011_COI	M22	1	BA99040_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99040	Sample	BA99040	targeted	Present	Sample	Upin_Bot2011_COI	M22	COI	45	BA99040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M22	CTTTAATTACAGCGTTTTTATTGTTGT	144.92811
BB10185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81392	WGS84	BB10185	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.319	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10185_Cmae_Bot2011_COI	AS4	1	BB10185_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10185	Sample	BB10185	targeted	Present	Sample	Cmae_Bot2011_COI	AS4	COI	45	BB10185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS4	ATGAACAGTCTATCCTCCTTTAG	138.51292
BB10189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81784	WGS84	BB10189	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.494	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10189_Cmae_Bot2011_COI	AS8	1	BB10189_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10189	Sample	BB10189	targeted	Present	Sample	Cmae_Bot2011_COI	AS8	COI	45	BB10189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS8	ATGAACAGTCTATCCTCCTTTAG	138.5121
BB10192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.8304	WGS84	BB10192	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.5	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10192_Cmae_Bot2011_COI	AS11	1	BB10192_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10192	Sample	BB10192	targeted	Present	Sample	Cmae_Bot2011_COI	AS11	COI	45	BB10192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS11	ATGAACAGTCTATCCTCCTTTAG	138.50724
BB10196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.80937	WGS84	BB10196	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.029	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10196_Cmae_Bot2011_COI	AS15	1	BB10196_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10196	Sample	BB10196	targeted	Present	Sample	Cmae_Bot2011_COI	AS15	COI	45	BB10196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS15	ATGAACAGTCTATCCTCCTTTAG	138.51838
BB10200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.80911	WGS84	BB10200	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.418	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10200_Cmae_Bot2011_COI	AS19	1	BB10200_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10200	Sample	BB10200	targeted	Present	Sample	Cmae_Bot2011_COI	AS19	COI	45	BB10200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS19	ATGAACAGTCTATCCTCCTTTAG	138.51731
BB10204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.82393	WGS84	BB10204	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.663	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10204_Cmae_Bot2011_COI	AS23	1	BB10204_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10204	Sample	BB10204	targeted	Present	Sample	Cmae_Bot2011_COI	AS23	COI	45	BB10204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS23	ATGAACAGTCTATCCTCCTTTAG	138.50893
BB12679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88398	WGS84	BB12679	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	24.627	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12679_Cmae_Bot2011_COI	H37	1	BB12679_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12679	Sample	BB12679	targeted	Present	Sample	Cmae_Bot2011_COI	H37	COI	45	BB12679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H37	ATGAACAGTCTATCCTCCTTTAG	147.33726
BB12681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88622	WGS84	BB12681	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	22.271	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12681_Mgig_Bot2012_COI	H39	1	BB12681_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12681	Sample	BB12681	targeted	Present	Sample	Mgig_Bot2012_COI	H39	COI	45	BB12681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H39	TCTTATTCGTTGGAGACTTTATAACCCT	147.33941
BB12682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.84406	WGS84	BB12682	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.423	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12682_Cmae_Bot2011_COI	H40	1	BB12682_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12682	Sample	BB12682	targeted	Present	Sample	Cmae_Bot2011_COI	H40	COI	45	BB12682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H40	ATGAACAGTCTATCCTCCTTTAG	147.33059
BB12685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88086	WGS84	BB12685	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	21.935	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12685_Mgig_Bot2012_COI	H43	1	BB12685_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12685	Sample	BB12685	targeted	Present	Sample	Mgig_Bot2012_COI	H43	COI	45	BB12685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H43	TCTTATTCGTTGGAGACTTTATAACCCT	147.34152
BB12686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88485	WGS84	BB12686	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	24.767	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12686_Cmae_Bot2011_COI	H44	1	BB12686_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12686	Sample	BB12686	targeted	Present	Sample	Cmae_Bot2011_COI	H44	COI	45	BB12686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H44	ATGAACAGTCTATCCTCCTTTAG	147.33488
BB12689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87697	WGS84	BB12689	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.937	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12689_Mgig_Bot2012_COI	H47	1	BB12689_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12689	Sample	BB12689	targeted	Present	Sample	Mgig_Bot2012_COI	H47	COI	45	BB12689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H47	TCTTATTCGTTGGAGACTTTATAACCCT	147.34149
BB12692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88285	WGS84	BB12692	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	20.607	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12692_Mgig_Bot2012_COI	H50	1	BB12692_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12692	Sample	BB12692	targeted	Present	Sample	Mgig_Bot2012_COI	H50	COI	45	BB12692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H50	TCTTATTCGTTGGAGACTTTATAACCCT	147.33683
BB12693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.82529	WGS84	BB12693	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.559	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12693_Cmae_Bot2011_COI	H51	1	BB12693_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12693	Sample	BB12693	targeted	Present	Sample	Cmae_Bot2011_COI	H51	COI	45	BB12693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H51	ATGAACAGTCTATCCTCCTTTAG	147.30513
BB12696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88292	WGS84	BB12696	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.897	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12696_Mgig_Bot2012_COI	H54	1	BB12696_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12696	Sample	BB12696	targeted	Present	Sample	Mgig_Bot2012_COI	H54	COI	45	BB12696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H54	TCTTATTCGTTGGAGACTTTATAACCCT	147.33613
BB16134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.82682	WGS84	BB16134	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB16134_Aamu_Bax2006_COI	AS42	1	BB16134_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16134	Sample	BB16134	targeted	Absent	Sample	Aamu_Bax2006_COI	AS42	COI	45	BB16134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS42	GCACAACCGGGATCTTTACTTC	138.50958
BB16134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.82682	WGS84	BB16134	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.48899841	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB16134_Sspa_Oph2007_COI	AS42	1	BB16134_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16134	Sample	BB16134	targeted	Present	Sample	Sspa_Oph2007_COI	AS42	COI	45	BB16134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS42	GAAGGCCTTCCGATGATC	138.50958
BB17781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84763	WGS84	BB17781	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.285	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17781_Mgig_Bot2012_COI	S38	1	BB17781_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17781	Sample	BB17781	targeted	Present	Sample	Mgig_Bot2012_COI	S38	COI	45	BB17781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S38	TCTTATTCGTTGGAGACTTTATAACCCT	151.2748
BB17785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84507	WGS84	BB17785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.388	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17785_Mgig_Bot2012_COI	S42	1	BB17785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17785	Sample	BB17785	targeted	Present	Sample	Mgig_Bot2012_COI	S42	COI	45	BB17785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S42	TCTTATTCGTTGGAGACTTTATAACCCT	151.27772
BB17789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84025	WGS84	BB17789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.747	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17789_Mgig_Bot2012_COI	S46	1	BB17789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17789	Sample	BB17789	targeted	Present	Sample	Mgig_Bot2012_COI	S46	COI	45	BB17789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S46	TCTTATTCGTTGGAGACTTTATAACCCT	151.25423
BB17792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84835	WGS84	BB17792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.316	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17792_Mgig_Bot2012_COI	S49	1	BB17792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17792	Sample	BB17792	targeted	Present	Sample	Mgig_Bot2012_COI	S49	COI	45	BB17792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S49	TCTTATTCGTTGGAGACTTTATAACCCT	151.27371
BB17796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84006	WGS84	BB17796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.37	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17796_Mgig_Bot2012_COI	S53	1	BB17796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17796	Sample	BB17796	targeted	Present	Sample	Mgig_Bot2012_COI	S53	COI	45	BB17796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S53	TCTTATTCGTTGGAGACTTTATAACCCT	151.25398
BB32903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01742	WGS84	BB32903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32903_Aamu_Bax2006_COI	PS-A02	1	BB32903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32903	Sample	BB32903	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A02	COI	45	BB32903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A02	GCACAACCGGGATCTTTACTTC	137.59663
BB32903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01742	WGS84	BB32903	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32903_Pcan_Bot2011_IGS	PS-A02	1	BB32903_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32903	Sample	BB32903	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A02	IGS	45	BB32903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A02	CGTAATCCTCAGTACTGGCTA	137.59663
BB32903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01742	WGS84	BB32903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.66200066	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32903_Sspa_Oph2007_COI	PS-A02	1	BB32903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32903	Sample	BB32903	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A02	COI	45	BB32903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A02	GAAGGCCTTCCGATGATC	137.59663
BB32903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01742	WGS84	BB32903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32903_Upin_Bot2011_COI	PS-A02	1	BB32903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32903	Sample	BB32903	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A02	COI	45	BB32903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A02	CTTTAATTACAGCGTTTTTATTGTTGT	137.59663
BB32904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01922	WGS84	BB32904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32904_Mgig_Bot2012_COI	PS-A03	1	BB32904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32904	Sample	BB32904	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A03	COI	45	BB32904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A03	TCTTATTCGTTGGAGACTTTATAACCCT	137.59862
BB32905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02878	WGS84	BB32905	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32905_Cmae_Bot2011_COI	PS-A04	1	BB32905_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32905	Sample	BB32905	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A04	COI	45	BB32905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A04	ATGAACAGTCTATCCTCCTTTAG	137.60044
BB32905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02878	WGS84	BB32905	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32905_Msal_Bot2012_COI	PS-A04	1	BB32905_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32905	Sample	BB32905	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A04	COI	45	BB32905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A04	GYTAGTTCCRATGATGTTAGCTG	137.60044
BB32907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03665	WGS84	BB32907	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32907_Aamu_Bax2006_COI	PS-A06	1	BB32907_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32907	Sample	BB32907	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A06	COI	45	BB32907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A06	GCACAACCGGGATCTTTACTTC	137.59798
BB32907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03665	WGS84	BB32907	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32907_Pcan_Bot2011_IGS	PS-A06	1	BB32907_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32907	Sample	BB32907	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A06	IGS	45	BB32907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A06	CGTAATCCTCAGTACTGGCTA	137.59798
BB32907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03665	WGS84	BB32907	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.77099991	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32907_Sspa_Oph2007_COI	PS-A06	1	BB32907_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32907	Sample	BB32907	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A06	COI	45	BB32907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A06	GAAGGCCTTCCGATGATC	137.59798
BB32907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03665	WGS84	BB32907	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32907_Upin_Bot2011_COI	PS-A06	1	BB32907_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32907	Sample	BB32907	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A06	COI	45	BB32907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A06	CTTTAATTACAGCGTTTTTATTGTTGT	137.59798
BB32908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03939	WGS84	BB32908	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32908_Mgig_Bot2012_COI	PS-A07	1	BB32908_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32908	Sample	BB32908	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A07	COI	45	BB32908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A07	TCTTATTCGTTGGAGACTTTATAACCCT	137.59628
BB32909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04109	WGS84	BB32909	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32909_Cmae_Bot2011_COI	PS-A08	1	BB32909_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32909	Sample	BB32909	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A08	COI	45	BB32909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A08	ATGAACAGTCTATCCTCCTTTAG	137.59478
BB32909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04109	WGS84	BB32909	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32909_Msal_Bot2012_COI	PS-A08	1	BB32909_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32909	Sample	BB32909	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A08	COI	45	BB32909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A08	GYTAGTTCCRATGATGTTAGCTG	137.59478
BB32910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03907	WGS84	BB32910	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32910_Aamu_Bax2006_COI	PS-A09	1	BB32910_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32910	Sample	BB32910	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A09	COI	45	BB32910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A09	GCACAACCGGGATCTTTACTTC	137.59498
BB32910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03907	WGS84	BB32910	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32910_Pcan_Bot2011_IGS	PS-A09	1	BB32910_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32910	Sample	BB32910	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A09	IGS	45	BB32910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A09	CGTAATCCTCAGTACTGGCTA	137.59498
BB32910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03907	WGS84	BB32910	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.95400047	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32910_Sspa_Oph2007_COI	PS-A09	1	BB32910_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32910	Sample	BB32910	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A09	COI	45	BB32910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A09	GAAGGCCTTCCGATGATC	137.59498
BB32910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03907	WGS84	BB32910	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32910_Upin_Bot2011_COI	PS-A09	1	BB32910_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32910	Sample	BB32910	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A09	COI	45	BB32910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A09	CTTTAATTACAGCGTTTTTATTGTTGT	137.59498
BB32911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03909	WGS84	BB32911	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32911_Mgig_Bot2012_COI	PS-A10	1	BB32911_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32911	Sample	BB32911	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A10	COI	45	BB32911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A10	TCTTATTCGTTGGAGACTTTATAACCCT	137.59297
BB32912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01736	WGS84	BB32912	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32912_Cmae_Bot2011_COI	PS-A11	1	BB32912_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32912	Sample	BB32912	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A11	COI	45	BB32912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A11	ATGAACAGTCTATCCTCCTTTAG	137.60109
BB32912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01736	WGS84	BB32912	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32912_Msal_Bot2012_COI	PS-A11	1	BB32912_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32912	Sample	BB32912	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A11	COI	45	BB32912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A11	GYTAGTTCCRATGATGTTAGCTG	137.60109
BB32914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01807	WGS84	BB32914	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32914_Aamu_Bax2006_COI	PS-A13	1	BB32914_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32914	Sample	BB32914	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A13	COI	45	BB32914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A13	GCACAACCGGGATCTTTACTTC	137.59817
BB32914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01807	WGS84	BB32914	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32914_Pcan_Bot2011_IGS	PS-A13	1	BB32914_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32914	Sample	BB32914	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A13	IGS	45	BB32914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A13	CGTAATCCTCAGTACTGGCTA	137.59817
BB32914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01807	WGS84	BB32914	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.83499908	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32914_Sspa_Oph2007_COI	PS-A13	1	BB32914_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32914	Sample	BB32914	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A13	COI	45	BB32914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A13	GAAGGCCTTCCGATGATC	137.59817
BB32914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01807	WGS84	BB32914	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32914_Upin_Bot2011_COI	PS-A13	1	BB32914_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32914	Sample	BB32914	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A13	COI	45	BB32914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A13	CTTTAATTACAGCGTTTTTATTGTTGT	137.59817
BB32915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01772	WGS84	BB32915	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32915_Mgig_Bot2012_COI	PS-A14	1	BB32915_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32915	Sample	BB32915	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A14	COI	45	BB32915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A14	TCTTATTCGTTGGAGACTTTATAACCCT	137.59601
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01845	WGS84	BB32916	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32916_Cmae_Bot2011_COI	PS-A15	1	BB32916_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	ATGAACAGTCTATCCTCCTTTAG	137.59697
BB32916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01845	WGS84	BB32916	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32916_Msal_Bot2012_COI	PS-A15	1	BB32916_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32916	Sample	BB32916	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A15	COI	45	BB32916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A15	GYTAGTTCCRATGATGTTAGCTG	137.59697
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02044	WGS84	BB32918	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32918_Aamu_Bax2006_COI	PS-A17	1	BB32918_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	GCACAACCGGGATCTTTACTTC	137.60071
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02044	WGS84	BB32918	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32918_Pcan_Bot2011_IGS	PS-A17	1	BB32918_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A17	IGS	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	CGTAATCCTCAGTACTGGCTA	137.60071
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02044	WGS84	BB32918	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.67000008	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32918_Sspa_Oph2007_COI	PS-A17	1	BB32918_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	GAAGGCCTTCCGATGATC	137.60071
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02044	WGS84	BB32918	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32918_Upin_Bot2011_COI	PS-A17	1	BB32918_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	CTTTAATTACAGCGTTTTTATTGTTGT	137.60071
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.02044	WGS84	BB32918	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32918_Vgib_Wil2025_COI	PS-A17	1	BB32918_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	CCCCTCTTTACAAGTTCTTTAGGGT	137.60071
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.02985	WGS84	BB32919	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32919_Asen_Wil2025_COI	PS-A18	1	BB32919_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	GTTGTTGTTACAACACATGCTCTTTTG	137.60018
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02985	WGS84	BB32919	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32919_Mgig_Bot2012_COI	PS-A18	1	BB32919_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	TCTTATTCGTTGGAGACTTTATAACCCT	137.60018
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03389	WGS84	BB32921	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32921_Aamu_Bax2006_COI	PS-A20	1	BB32921_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	GCACAACCGGGATCTTTACTTC	137.60019
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03389	WGS84	BB32921	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32921_Pcan_Bot2011_IGS	PS-A20	1	BB32921_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A20	IGS	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	CGTAATCCTCAGTACTGGCTA	137.60019
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03389	WGS84	BB32921	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.70000076	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32921_Sspa_Oph2007_COI	PS-A20	1	BB32921_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	GAAGGCCTTCCGATGATC	137.60019
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03389	WGS84	BB32921	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32921_Upin_Bot2011_COI	PS-A20	1	BB32921_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	CTTTAATTACAGCGTTTTTATTGTTGT	137.60019
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03389	WGS84	BB32921	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32921_Vgib_Wil2025_COI	PS-A20	1	BB32921_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	CCCCTCTTTACAAGTTCTTTAGGGT	137.60019
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03692	WGS84	BB32922	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32922_Asen_Wil2025_COI	PS-A21	1	BB32922_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	GTTGTTGTTACAACACATGCTCTTTTG	137.59936
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03692	WGS84	BB32922	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32922_Mgig_Bot2012_COI	PS-A21	1	BB32922_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	TCTTATTCGTTGGAGACTTTATAACCCT	137.59936
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03696	WGS84	BB32923	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32923_Cmae_Bot2011_COI	PS-A22	1	BB32923_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	ATGAACAGTCTATCCTCCTTTAG	137.59708
BB32923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03696	WGS84	BB32923	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32923_Msal_Bot2012_COI	PS-A22	1	BB32923_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32923	Sample	BB32923	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A22	COI	45	BB32923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A22	GYTAGTTCCRATGATGTTAGCTG	137.59708
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04269	WGS84	BB32925	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32925_Aamu_Bax2006_COI	PS-A24	1	BB32925_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	GCACAACCGGGATCTTTACTTC	137.59376
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04269	WGS84	BB32925	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32925_Pcan_Bot2011_IGS	PS-A24	1	BB32925_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A24	IGS	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	CGTAATCCTCAGTACTGGCTA	137.59376
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04269	WGS84	BB32925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.97200012	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32925_Sspa_Oph2007_COI	PS-A24	1	BB32925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	GAAGGCCTTCCGATGATC	137.59376
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04269	WGS84	BB32925	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32925_Upin_Bot2011_COI	PS-A24	1	BB32925_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	CTTTAATTACAGCGTTTTTATTGTTGT	137.59376
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04269	WGS84	BB32925	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32925_Vgib_Wil2025_COI	PS-A24	1	BB32925_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	CCCCTCTTTACAAGTTCTTTAGGGT	137.59376
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.0418	WGS84	BB32926	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32926_Asen_Wil2025_COI	PS-A25	1	BB32926_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	GTTGTTGTTACAACACATGCTCTTTTG	137.59386
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.0418	WGS84	BB32926	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32926_Mgig_Bot2012_COI	PS-A25	1	BB32926_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	TCTTATTCGTTGGAGACTTTATAACCCT	137.59386
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04086	WGS84	BB32927	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32927_Cmae_Bot2011_COI	PS-A26	1	BB32927_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	ATGAACAGTCTATCCTCCTTTAG	137.59588
BB32927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04086	WGS84	BB32927	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32927_Msal_Bot2012_COI	PS-A26	1	BB32927_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32927	Sample	BB32927	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A26	COI	45	BB32927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A26	GYTAGTTCCRATGATGTTAGCTG	137.59588
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04087	WGS84	BB32929	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32929_Aamu_Bax2006_COI	PS-A28	1	BB32929_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	GCACAACCGGGATCTTTACTTC	137.59434
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04087	WGS84	BB32929	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32929_Pcan_Bot2011_IGS	PS-A28	1	BB32929_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A28	IGS	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	CGTAATCCTCAGTACTGGCTA	137.59434
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04087	WGS84	BB32929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.15000153	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32929_Sspa_Oph2007_COI	PS-A28	1	BB32929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	GAAGGCCTTCCGATGATC	137.59434
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04087	WGS84	BB32929	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32929_Upin_Bot2011_COI	PS-A28	1	BB32929_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	CTTTAATTACAGCGTTTTTATTGTTGT	137.59434
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04087	WGS84	BB32929	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32929_Vgib_Wil2025_COI	PS-A28	1	BB32929_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	CCCCTCTTTACAAGTTCTTTAGGGT	137.59434
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03918	WGS84	BB32930	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32930_Cmae_Bot2011_COI	PS-A29	1	BB32930_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	ATGAACAGTCTATCCTCCTTTAG	137.59354
BB32930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03918	WGS84	BB32930	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32930_Msal_Bot2012_COI	PS-A29	1	BB32930_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32930	Sample	BB32930	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A29	COI	45	BB32930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A29	GYTAGTTCCRATGATGTTAGCTG	137.59354
BB32937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71952	WGS84	BB32937	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32937_Mgig_Bot2012_COI	PS-B01	1	BB32937_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32937	Sample	BB32937	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B01	COI	45	BB32937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B01	TCTTATTCGTTGGAGACTTTATAACCCT	135.86182
BB32938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72023	WGS84	BB32938	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32938_Cmae_Bot2011_COI	PS-B02	1	BB32938_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32938	Sample	BB32938	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B02	COI	45	BB32938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B02	ATGAACAGTCTATCCTCCTTTAG	135.86471
BB32938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72023	WGS84	BB32938	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32938_Msal_Bot2012_COI	PS-B02	1	BB32938_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32938	Sample	BB32938	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B02	COI	45	BB32938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B02	GYTAGTTCCRATGATGTTAGCTG	135.86471
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71539	WGS84	BB32940	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32940_Asen_Wil2025_COI	PS-B04	1	BB32940_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	GTTGTTGTTACAACACATGCTCTTTTG	135.86716
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71539	WGS84	BB32940	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32940_Mgig_Bot2012_COI	PS-B04	1	BB32940_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	TCTTATTCGTTGGAGACTTTATAACCCT	135.86716
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71512	WGS84	BB32941	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32941_Cmae_Bot2011_COI	PS-B05	1	BB32941_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	ATGAACAGTCTATCCTCCTTTAG	135.87171
BB32941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71512	WGS84	BB32941	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32941_Msal_Bot2012_COI	PS-B05	1	BB32941_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32941	Sample	BB32941	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B05	COI	45	BB32941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B05	GYTAGTTCCRATGATGTTAGCTG	135.87171
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72029	WGS84	BB32943	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32943_Aamu_Bax2006_COI	PS-B07	1	BB32943_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	GCACAACCGGGATCTTTACTTC	135.87318
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72029	WGS84	BB32943	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32943_Pcan_Bot2011_IGS	PS-B07	1	BB32943_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B07	IGS	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	CGTAATCCTCAGTACTGGCTA	135.87318
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72029	WGS84	BB32943	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32943_Sspa_Oph2007_COI	PS-B07	1	BB32943_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	GAAGGCCTTCCGATGATC	135.87318
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72029	WGS84	BB32943	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32943_Upin_Bot2011_COI	PS-B07	1	BB32943_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	CTTTAATTACAGCGTTTTTATTGTTGT	135.87318
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.72029	WGS84	BB32943	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32943_Vgib_Wil2025_COI	PS-B07	1	BB32943_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	CCCCTCTTTACAAGTTCTTTAGGGT	135.87318
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71838	WGS84	BB32944	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32944_Asen_Wil2025_COI	PS-B08	1	BB32944_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	GTTGTTGTTACAACACATGCTCTTTTG	135.87715
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71838	WGS84	BB32944	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32944_Mgig_Bot2012_COI	PS-B08	1	BB32944_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	TCTTATTCGTTGGAGACTTTATAACCCT	135.87715
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72015	WGS84	BB32945	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32945_Cmae_Bot2011_COI	PS-B09	1	BB32945_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	ATGAACAGTCTATCCTCCTTTAG	135.8806
BB32945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72015	WGS84	BB32945	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32945_Msal_Bot2012_COI	PS-B09	1	BB32945_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32945	Sample	BB32945	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B09	COI	45	BB32945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B09	GYTAGTTCCRATGATGTTAGCTG	135.8806
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71863	WGS84	BB32947	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32947_Aamu_Bax2006_COI	PS-B11	1	BB32947_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	GCACAACCGGGATCTTTACTTC	135.86087
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71863	WGS84	BB32947	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32947_Pcan_Bot2011_IGS	PS-B11	1	BB32947_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B11	IGS	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	CGTAATCCTCAGTACTGGCTA	135.86087
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71863	WGS84	BB32947	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32947_Sspa_Oph2007_COI	PS-B11	1	BB32947_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	GAAGGCCTTCCGATGATC	135.86087
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71863	WGS84	BB32947	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32947_Upin_Bot2011_COI	PS-B11	1	BB32947_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	CTTTAATTACAGCGTTTTTATTGTTGT	135.86087
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71863	WGS84	BB32947	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32947_Vgib_Wil2025_COI	PS-B11	1	BB32947_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	CCCCTCTTTACAAGTTCTTTAGGGT	135.86087
BB32948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72021	WGS84	BB32948	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32948_Mgig_Bot2012_COI	PS-B12	1	BB32948_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32948	Sample	BB32948	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B12	COI	45	BB32948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B12	TCTTATTCGTTGGAGACTTTATAACCCT	135.8631
BB32949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72033	WGS84	BB32949	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32949_Cmae_Bot2011_COI	PS-B13	1	BB32949_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32949	Sample	BB32949	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B13	COI	45	BB32949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B13	ATGAACAGTCTATCCTCCTTTAG	135.86433
BB32949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72033	WGS84	BB32949	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32949_Msal_Bot2012_COI	PS-B13	1	BB32949_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32949	Sample	BB32949	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B13	COI	45	BB32949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B13	GYTAGTTCCRATGATGTTAGCTG	135.86433
BB32950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72022	WGS84	BB32950	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32950_Aamu_Bax2006_COI	PS-B14	1	BB32950_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32950	Sample	BB32950	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B14	COI	45	BB32950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B14	GCACAACCGGGATCTTTACTTC	135.86322
BB32950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72022	WGS84	BB32950	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32950_Pcan_Bot2011_IGS	PS-B14	1	BB32950_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32950	Sample	BB32950	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B14	IGS	45	BB32950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B14	CGTAATCCTCAGTACTGGCTA	135.86322
BB32950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72022	WGS84	BB32950	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.47299957	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32950_Sspa_Oph2007_COI	PS-B14	1	BB32950_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32950	Sample	BB32950	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B14	COI	45	BB32950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B14	GAAGGCCTTCCGATGATC	135.86322
BB32950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72022	WGS84	BB32950	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32950_Upin_Bot2011_COI	PS-B14	1	BB32950_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32950	Sample	BB32950	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B14	COI	45	BB32950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B14	CTTTAATTACAGCGTTTTTATTGTTGT	135.86322
BB32951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71958	WGS84	BB32951	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32951_Mgig_Bot2012_COI	PS-B15	1	BB32951_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32951	Sample	BB32951	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B15	COI	45	BB32951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B15	TCTTATTCGTTGGAGACTTTATAACCCT	135.86192
BB32952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71716	WGS84	BB32952	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32952_Cmae_Bot2011_COI	PS-B16	1	BB32952_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32952	Sample	BB32952	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B16	COI	45	BB32952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B16	ATGAACAGTCTATCCTCCTTTAG	135.8641
BB32952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71716	WGS84	BB32952	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32952_Msal_Bot2012_COI	PS-B16	1	BB32952_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32952	Sample	BB32952	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B16	COI	45	BB32952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B16	GYTAGTTCCRATGATGTTAGCTG	135.8641
BB32954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71403	WGS84	BB32954	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32954_Aamu_Bax2006_COI	PS-B18	1	BB32954_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32954	Sample	BB32954	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B18	COI	45	BB32954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B18	GCACAACCGGGATCTTTACTTC	135.86807
BB32954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71403	WGS84	BB32954	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32954_Pcan_Bot2011_IGS	PS-B18	1	BB32954_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32954	Sample	BB32954	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B18	IGS	45	BB32954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B18	CGTAATCCTCAGTACTGGCTA	135.86807
BB32954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71403	WGS84	BB32954	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.74499893	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32954_Sspa_Oph2007_COI	PS-B18	1	BB32954_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32954	Sample	BB32954	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B18	COI	45	BB32954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B18	GAAGGCCTTCCGATGATC	135.86807
BB32954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71403	WGS84	BB32954	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32954_Upin_Bot2011_COI	PS-B18	1	BB32954_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32954	Sample	BB32954	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B18	COI	45	BB32954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B18	CTTTAATTACAGCGTTTTTATTGTTGT	135.86807
BB32955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71395	WGS84	BB32955	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32955_Mgig_Bot2012_COI	PS-B19	1	BB32955_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32955	Sample	BB32955	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B19	COI	45	BB32955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B19	TCTTATTCGTTGGAGACTTTATAACCCT	135.87171
BB32956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71632	WGS84	BB32956	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32956_Cmae_Bot2011_COI	PS-B20	1	BB32956_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32956	Sample	BB32956	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B20	COI	45	BB32956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B20	ATGAACAGTCTATCCTCCTTTAG	135.87171
BB32956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71632	WGS84	BB32956	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32956_Msal_Bot2012_COI	PS-B20	1	BB32956_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32956	Sample	BB32956	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B20	COI	45	BB32956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B20	GYTAGTTCCRATGATGTTAGCTG	135.87171
BB32958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72017	WGS84	BB32958	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32958_Aamu_Bax2006_COI	PS-B22	1	BB32958_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32958	Sample	BB32958	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B22	COI	45	BB32958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B22	GCACAACCGGGATCTTTACTTC	135.87103
BB32958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72017	WGS84	BB32958	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32958_Pcan_Bot2011_IGS	PS-B22	1	BB32958_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32958	Sample	BB32958	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B22	IGS	45	BB32958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B22	CGTAATCCTCAGTACTGGCTA	135.87103
BB32958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72017	WGS84	BB32958	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.76699829	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32958_Sspa_Oph2007_COI	PS-B22	1	BB32958_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32958	Sample	BB32958	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B22	COI	45	BB32958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B22	GAAGGCCTTCCGATGATC	135.87103
BB32958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72017	WGS84	BB32958	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32958_Upin_Bot2011_COI	PS-B22	1	BB32958_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32958	Sample	BB32958	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B22	COI	45	BB32958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B22	CTTTAATTACAGCGTTTTTATTGTTGT	135.87103
BB32959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72081	WGS84	BB32959	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32959_Mgig_Bot2012_COI	PS-B23	1	BB32959_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32959	Sample	BB32959	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B23	COI	45	BB32959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B23	TCTTATTCGTTGGAGACTTTATAACCCT	135.87177
BB32961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71855	WGS84	BB32961	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32961_Aamu_Bax2006_COI	PS-B25	1	BB32961_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32961	Sample	BB32961	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B25	COI	45	BB32961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B25	GCACAACCGGGATCTTTACTTC	135.87565
BB32961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71855	WGS84	BB32961	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32961_Pcan_Bot2011_IGS	PS-B25	1	BB32961_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32961	Sample	BB32961	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B25	IGS	45	BB32961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B25	CGTAATCCTCAGTACTGGCTA	135.87565
BB32961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71855	WGS84	BB32961	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32961_Sspa_Oph2007_COI	PS-B25	1	BB32961_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32961	Sample	BB32961	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B25	COI	45	BB32961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B25	GAAGGCCTTCCGATGATC	135.87565
BB32961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71855	WGS84	BB32961	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32961_Upin_Bot2011_COI	PS-B25	1	BB32961_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32961	Sample	BB32961	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B25	COI	45	BB32961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B25	CTTTAATTACAGCGTTTTTATTGTTGT	135.87565
BB32962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71858	WGS84	BB32962	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32962_Mgig_Bot2012_COI	PS-B26	1	BB32962_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32962	Sample	BB32962	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B26	COI	45	BB32962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B26	TCTTATTCGTTGGAGACTTTATAACCCT	135.87847
BB32963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71932	WGS84	BB32963	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32963_Cmae_Bot2011_COI	PS-B27	1	BB32963_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32963	Sample	BB32963	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B27	COI	45	BB32963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B27	ATGAACAGTCTATCCTCCTTTAG	135.87964
BB32963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71932	WGS84	BB32963	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32963_Msal_Bot2012_COI	PS-B27	1	BB32963_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32963	Sample	BB32963	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B27	COI	45	BB32963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B27	GYTAGTTCCRATGATGTTAGCTG	135.87964
BB32965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72362	WGS84	BB32965	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32965_Aamu_Bax2006_COI	PS-B29	1	BB32965_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32965	Sample	BB32965	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B29	COI	45	BB32965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B29	GCACAACCGGGATCTTTACTTC	135.88477
BB32965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72362	WGS84	BB32965	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32965_Pcan_Bot2011_IGS	PS-B29	1	BB32965_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32965	Sample	BB32965	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B29	IGS	45	BB32965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B29	CGTAATCCTCAGTACTGGCTA	135.88477
BB32965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72362	WGS84	BB32965	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32965_Sspa_Oph2007_COI	PS-B29	1	BB32965_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32965	Sample	BB32965	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B29	COI	45	BB32965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B29	GAAGGCCTTCCGATGATC	135.88477
BB32965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72362	WGS84	BB32965	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32965_Upin_Bot2011_COI	PS-B29	1	BB32965_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32965	Sample	BB32965	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B29	COI	45	BB32965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B29	CTTTAATTACAGCGTTTTTATTGTTGT	135.88477
BB32966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72586	WGS84	BB32966	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32966_Mgig_Bot2012_COI	PS-B30	1	BB32966_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32966	Sample	BB32966	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B30	COI	45	BB32966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B30	TCTTATTCGTTGGAGACTTTATAACCCT	135.88634
BB32972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93052	WGS84	BB32972	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32972_Aamu_Bax2006_COI	PS-C01	1	BB32972_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32972	Sample	BB32972	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C01	COI	45	BB32972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C01	GCACAACCGGGATCTTTACTTC	137.61115
BB32972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93052	WGS84	BB32972	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32972_Pcan_Bot2011_IGS	PS-C01	1	BB32972_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32972	Sample	BB32972	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C01	IGS	45	BB32972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C01	CGTAATCCTCAGTACTGGCTA	137.61115
BB32972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93052	WGS84	BB32972	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32972_Sspa_Oph2007_COI	PS-C01	1	BB32972_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32972	Sample	BB32972	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C01	COI	45	BB32972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C01	GAAGGCCTTCCGATGATC	137.61115
BB32972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93052	WGS84	BB32972	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32972_Upin_Bot2011_COI	PS-C01	1	BB32972_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32972	Sample	BB32972	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C01	COI	45	BB32972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C01	CTTTAATTACAGCGTTTTTATTGTTGT	137.61115
BB32973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93135	WGS84	BB32973	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32973_Mgig_Bot2012_COI	PS-C02	1	BB32973_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32973	Sample	BB32973	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C02	COI	45	BB32973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C02	TCTTATTCGTTGGAGACTTTATAACCCT	137.60781
BB32974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92647	WGS84	BB32974	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32974_Cmae_Bot2011_COI	PS-C03	1	BB32974_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32974	Sample	BB32974	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C03	COI	45	BB32974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C03	ATGAACAGTCTATCCTCCTTTAG	137.61272
BB32974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92647	WGS84	BB32974	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32974_Msal_Bot2012_COI	PS-C03	1	BB32974_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32974	Sample	BB32974	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C03	COI	45	BB32974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C03	GYTAGTTCCRATGATGTTAGCTG	137.61272
BB32976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92643	WGS84	BB32976	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32976_Aamu_Bax2006_COI	PS-C05	1	BB32976_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32976	Sample	BB32976	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C05	COI	45	BB32976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C05	GCACAACCGGGATCTTTACTTC	137.62
BB32976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92643	WGS84	BB32976	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32976_Pcan_Bot2011_IGS	PS-C05	1	BB32976_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32976	Sample	BB32976	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C05	IGS	45	BB32976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C05	CGTAATCCTCAGTACTGGCTA	137.62
BB32976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92643	WGS84	BB32976	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32976_Sspa_Oph2007_COI	PS-C05	1	BB32976_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32976	Sample	BB32976	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C05	COI	45	BB32976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C05	GAAGGCCTTCCGATGATC	137.62
BB32976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92643	WGS84	BB32976	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32976_Upin_Bot2011_COI	PS-C05	1	BB32976_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32976	Sample	BB32976	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C05	COI	45	BB32976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C05	CTTTAATTACAGCGTTTTTATTGTTGT	137.62
BB32977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92558	WGS84	BB32977	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32977_Mgig_Bot2012_COI	PS-C06	1	BB32977_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32977	Sample	BB32977	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C06	COI	45	BB32977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C06	TCTTATTCGTTGGAGACTTTATAACCCT	137.62398
BB32978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92124	WGS84	BB32978	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32978_Cmae_Bot2011_COI	PS-C07	1	BB32978_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32978	Sample	BB32978	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C07	COI	45	BB32978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C07	ATGAACAGTCTATCCTCCTTTAG	137.62582
BB32978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92124	WGS84	BB32978	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32978_Msal_Bot2012_COI	PS-C07	1	BB32978_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32978	Sample	BB32978	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C07	COI	45	BB32978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C07	GYTAGTTCCRATGATGTTAGCTG	137.62582
BB32980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92255	WGS84	BB32980	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32980_Mgig_Bot2012_COI	PS-C09	1	BB32980_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32980	Sample	BB32980	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C09	COI	45	BB32980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C09	TCTTATTCGTTGGAGACTTTATAACCCT	137.63506
BB32981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92123	WGS84	BB32981	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32981_Cmae_Bot2011_COI	PS-C10	1	BB32981_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32981	Sample	BB32981	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C10	COI	45	BB32981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C10	ATGAACAGTCTATCCTCCTTTAG	137.63952
BB32981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92123	WGS84	BB32981	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32981_Msal_Bot2012_COI	PS-C10	1	BB32981_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32981	Sample	BB32981	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C10	COI	45	BB32981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C10	GYTAGTTCCRATGATGTTAGCTG	137.63952
BB32983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93096	WGS84	BB32983	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32983_Aamu_Bax2006_COI	PS-C12	1	BB32983_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32983	Sample	BB32983	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C12	COI	45	BB32983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C12	GCACAACCGGGATCTTTACTTC	137.61016
BB32983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93096	WGS84	BB32983	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32983_Pcan_Bot2011_IGS	PS-C12	1	BB32983_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32983	Sample	BB32983	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C12	IGS	45	BB32983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C12	CGTAATCCTCAGTACTGGCTA	137.61016
BB32983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93096	WGS84	BB32983	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32983_Sspa_Oph2007_COI	PS-C12	1	BB32983_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32983	Sample	BB32983	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C12	COI	45	BB32983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C12	GAAGGCCTTCCGATGATC	137.61016
BB32983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93096	WGS84	BB32983	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32983_Upin_Bot2011_COI	PS-C12	1	BB32983_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32983	Sample	BB32983	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C12	COI	45	BB32983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C12	CTTTAATTACAGCGTTTTTATTGTTGT	137.61016
BB32984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93119	WGS84	BB32984	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32984_Mgig_Bot2012_COI	PS-C13	1	BB32984_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32984	Sample	BB32984	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C13	COI	45	BB32984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C13	TCTTATTCGTTGGAGACTTTATAACCCT	137.609
BB32985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93162	WGS84	BB32985	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32985_Cmae_Bot2011_COI	PS-C14	1	BB32985_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32985	Sample	BB32985	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C14	COI	45	BB32985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C14	ATGAACAGTCTATCCTCCTTTAG	137.60666
BB32985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93162	WGS84	BB32985	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32985_Msal_Bot2012_COI	PS-C14	1	BB32985_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32985	Sample	BB32985	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C14	COI	45	BB32985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C14	GYTAGTTCCRATGATGTTAGCTG	137.60666
BB32987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92631	WGS84	BB32987	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32987_Aamu_Bax2006_COI	PS-C16	1	BB32987_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32987	Sample	BB32987	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C16	COI	45	BB32987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C16	GCACAACCGGGATCTTTACTTC	137.6116
BB32987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92631	WGS84	BB32987	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32987_Pcan_Bot2011_IGS	PS-C16	1	BB32987_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32987	Sample	BB32987	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C16	IGS	45	BB32987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C16	CGTAATCCTCAGTACTGGCTA	137.6116
BB32987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92631	WGS84	BB32987	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32987_Sspa_Oph2007_COI	PS-C16	1	BB32987_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32987	Sample	BB32987	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C16	COI	45	BB32987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C16	GAAGGCCTTCCGATGATC	137.6116
BB32987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92631	WGS84	BB32987	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32987_Upin_Bot2011_COI	PS-C16	1	BB32987_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32987	Sample	BB32987	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C16	COI	45	BB32987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C16	CTTTAATTACAGCGTTTTTATTGTTGT	137.6116
BB32988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92372	WGS84	BB32988	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32988_Mgig_Bot2012_COI	PS-C17	1	BB32988_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32988	Sample	BB32988	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C17	COI	45	BB32988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C17	TCTTATTCGTTGGAGACTTTATAACCCT	137.61164
BB32989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92406	WGS84	BB32989	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32989_Cmae_Bot2011_COI	PS-C18	1	BB32989_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32989	Sample	BB32989	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C18	COI	45	BB32989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C18	ATGAACAGTCTATCCTCCTTTAG	137.61464
BB32989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92406	WGS84	BB32989	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32989_Msal_Bot2012_COI	PS-C18	1	BB32989_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32989	Sample	BB32989	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C18	COI	45	BB32989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C18	GYTAGTTCCRATGATGTTAGCTG	137.61464
BB32990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92591	WGS84	BB32990	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32990_Aamu_Bax2006_COI	PS-C19	1	BB32990_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32990	Sample	BB32990	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C19	COI	45	BB32990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C19	GCACAACCGGGATCTTTACTTC	137.61858
BB32990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92591	WGS84	BB32990	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32990_Pcan_Bot2011_IGS	PS-C19	1	BB32990_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32990	Sample	BB32990	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C19	IGS	45	BB32990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C19	CGTAATCCTCAGTACTGGCTA	137.61858
BB32990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92591	WGS84	BB32990	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32990_Sspa_Oph2007_COI	PS-C19	1	BB32990_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32990	Sample	BB32990	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C19	COI	45	BB32990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C19	GAAGGCCTTCCGATGATC	137.61858
BB32990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92591	WGS84	BB32990	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32990_Upin_Bot2011_COI	PS-C19	1	BB32990_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32990	Sample	BB32990	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C19	COI	45	BB32990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C19	CTTTAATTACAGCGTTTTTATTGTTGT	137.61858
BB32991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.9266	WGS84	BB32991	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32991_Mgig_Bot2012_COI	PS-C20	1	BB32991_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32991	Sample	BB32991	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C20	COI	45	BB32991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C20	TCTTATTCGTTGGAGACTTTATAACCCT	137.62115
BB32992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92572	WGS84	BB32992	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32992_Cmae_Bot2011_COI	PS-C21	1	BB32992_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32992	Sample	BB32992	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C21	COI	45	BB32992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C21	ATGAACAGTCTATCCTCCTTTAG	137.62211
BB32992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92572	WGS84	BB32992	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32992_Msal_Bot2012_COI	PS-C21	1	BB32992_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32992	Sample	BB32992	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C21	COI	45	BB32992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C21	GYTAGTTCCRATGATGTTAGCTG	137.62211
BB32994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92304	WGS84	BB32994	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32994_Aamu_Bax2006_COI	PS-C23	1	BB32994_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32994	Sample	BB32994	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C23	COI	45	BB32994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C23	GCACAACCGGGATCTTTACTTC	137.62427
BB32994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92304	WGS84	BB32994	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32994_Pcan_Bot2011_IGS	PS-C23	1	BB32994_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32994	Sample	BB32994	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C23	IGS	45	BB32994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C23	CGTAATCCTCAGTACTGGCTA	137.62427
BB32994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92304	WGS84	BB32994	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32994_Sspa_Oph2007_COI	PS-C23	1	BB32994_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32994	Sample	BB32994	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C23	COI	45	BB32994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C23	GAAGGCCTTCCGATGATC	137.62427
BB32994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92304	WGS84	BB32994	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32994_Upin_Bot2011_COI	PS-C23	1	BB32994_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32994	Sample	BB32994	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C23	COI	45	BB32994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C23	CTTTAATTACAGCGTTTTTATTGTTGT	137.62427
BB32995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92047	WGS84	BB32995	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32995_Mgig_Bot2012_COI	PS-C24	1	BB32995_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32995	Sample	BB32995	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C24	COI	45	BB32995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C24	TCTTATTCGTTGGAGACTTTATAACCCT	137.62665
BB32996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92203	WGS84	BB32996	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32996_Cmae_Bot2011_COI	PS-C25	1	BB32996_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32996	Sample	BB32996	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C25	COI	45	BB32996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C25	ATGAACAGTCTATCCTCCTTTAG	137.62572
BB32996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92203	WGS84	BB32996	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32996_Msal_Bot2012_COI	PS-C25	1	BB32996_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32996	Sample	BB32996	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C25	COI	45	BB32996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C25	GYTAGTTCCRATGATGTTAGCTG	137.62572
BB32998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92361	WGS84	BB32998	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32998_Aamu_Bax2006_COI	PS-C27	1	BB32998_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32998	Sample	BB32998	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C27	COI	45	BB32998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C27	GCACAACCGGGATCTTTACTTC	137.63321
BB32998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92361	WGS84	BB32998	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32998_Pcan_Bot2011_IGS	PS-C27	1	BB32998_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32998	Sample	BB32998	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C27	IGS	45	BB32998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C27	CGTAATCCTCAGTACTGGCTA	137.63321
BB32998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92361	WGS84	BB32998	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32998_Sspa_Oph2007_COI	PS-C27	1	BB32998_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32998	Sample	BB32998	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C27	COI	45	BB32998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C27	GAAGGCCTTCCGATGATC	137.63321
BB32998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92361	WGS84	BB32998	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32998_Upin_Bot2011_COI	PS-C27	1	BB32998_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32998	Sample	BB32998	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C27	COI	45	BB32998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C27	CTTTAATTACAGCGTTTTTATTGTTGT	137.63321
BB32999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92169	WGS84	BB32999	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32999_Mgig_Bot2012_COI	PS-C28	1	BB32999_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32999	Sample	BB32999	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C28	COI	45	BB32999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C28	TCTTATTCGTTGGAGACTTTATAACCCT	137.63669
BB33001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.91952	WGS84	BB33001	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB33001_Aamu_Bax2006_COI	PS-C30	1	BB33001_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33001	Sample	BB33001	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C30	COI	45	BB33001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C30	GCACAACCGGGATCTTTACTTC	137.63993
BB33001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.91952	WGS84	BB33001	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB33001_Pcan_Bot2011_IGS	PS-C30	1	BB33001_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33001	Sample	BB33001	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C30	IGS	45	BB33001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C30	CGTAATCCTCAGTACTGGCTA	137.63993
BB33001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.91952	WGS84	BB33001	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB33001_Sspa_Oph2007_COI	PS-C30	1	BB33001_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33001	Sample	BB33001	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C30	COI	45	BB33001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C30	GAAGGCCTTCCGATGATC	137.63993
BB33001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.91952	WGS84	BB33001	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB33001_Upin_Bot2011_COI	PS-C30	1	BB33001_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33001	Sample	BB33001	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C30	COI	45	BB33001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C30	CTTTAATTACAGCGTTTTTATTGTTGT	137.63993
BB46885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.82076	WGS84	BB46885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.072	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB46885_Cmae_Bot2011_COI	M44	1	BB46885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46885	Sample	BB46885	targeted	Present	Sample	Cmae_Bot2011_COI	M44	COI	45	BB46885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M44	ATGAACAGTCTATCCTCCTTTAG	144.9125
BB46887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.83822	WGS84	BB46887	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.901	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46887_Upin_Bot2011_COI	M46	1	BB46887_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46887	Sample	BB46887	targeted	Present	Sample	Upin_Bot2011_COI	M46	COI	45	BB46887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M46	CTTTAATTACAGCGTTTTTATTGTTGT	144.89792
BB46890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84857	WGS84	BB46890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.429	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46890_Aamu_Bax2006_COI	M49	1	BB46890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46890	Sample	BB46890	targeted	Present	Sample	Aamu_Bax2006_COI	M49	COI	45	BB46890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M49	GCACAACCGGGATCTTTACTTC	144.91035
BB46890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84857	WGS84	BB46890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.96668	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46890_Sspa_Oph2007_COI	M49	1	BB46890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46890	Sample	BB46890	targeted	Present	Sample	Sspa_Oph2007_COI	M49	COI	45	BB46890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M49	GAAGGCCTTCCGATGATC	144.91035
BB46890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84857	WGS84	BB46890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.559	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46890_Upin_Bot2011_COI	M49	1	BB46890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46890	Sample	BB46890	targeted	Present	Sample	Upin_Bot2011_COI	M49	COI	45	BB46890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M49	CTTTAATTACAGCGTTTTTATTGTTGT	144.91035
BB46894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84244	WGS84	BB46894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.887	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46894_Upin_Bot2011_COI	M53	1	BB46894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46894	Sample	BB46894	targeted	Present	Sample	Upin_Bot2011_COI	M53	COI	45	BB46894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M53	CTTTAATTACAGCGTTTTTATTGTTGT	144.92301
BB46896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.82346	WGS84	BB46896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.29	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB46896_Cmae_Bot2011_COI	M55	1	BB46896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46896	Sample	BB46896	targeted	Present	Sample	Cmae_Bot2011_COI	M55	COI	45	BB46896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M55	ATGAACAGTCTATCCTCCTTTAG	144.90545
BB57685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77745	WGS84	BB57685	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57685_Aamu_Bax2006_COI	S1-02	1	BB57685_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57685	Sample	BB57685	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-02	COI	45	BB57685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-02	GCACAACCGGGATCTTTACTTC	138.51629
BB57685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77745	WGS84	BB57685	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57685_Pcan_Bot2011_IGS	S1-02	1	BB57685_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57685	Sample	BB57685	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-02	IGS	45	BB57685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-02	CGTAATCCTCAGTACTGGCTA	138.51629
BB57685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77745	WGS84	BB57685	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.45	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57685_Sspa_Oph2007_COI	S1-02	1	BB57685_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57685	Sample	BB57685	targeted	Present	Sample	Sspa_Oph2007_COI	S1-02	COI	45	BB57685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-02	GAAGGCCTTCCGATGATC	138.51629
BB57685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77745	WGS84	BB57685	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57685_Upin_Bot2011_COI	S1-02	1	BB57685_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57685	Sample	BB57685	targeted	Absent	Sample	Upin_Bot2011_COI	S1-02	COI	45	BB57685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.51629
BB57686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77915	WGS84	BB57686	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57686_Cjap_Sim2018_COI	S1-04	1	BB57686_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57686	Sample	BB57686	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-04	COI	45	BB57686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51673
BB57686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.77915	WGS84	BB57686	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.814	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57686_Mgig_Bot2012_COI	S1-04	1	BB57686_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57686	Sample	BB57686	targeted	Present	Sample	Mgig_Bot2012_COI	S1-04	COI	45	BB57686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.51673
BB57687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84686	WGS84	BB57687	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57687_Cmae_Bot2011_COI	S1-07	1	BB57687_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57687	Sample	BB57687	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-07	COI	45	BB57687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-07	ATGAACAGTCTATCCTCCTTTAG	138.49747
BB57689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8371	WGS84	BB57689	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57689_Aamu_Bax2006_COI	S1-12	1	BB57689_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57689	Sample	BB57689	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-12	COI	45	BB57689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-12	GCACAACCGGGATCTTTACTTC	138.5104
BB57689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8371	WGS84	BB57689	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57689_Pcan_Bot2011_IGS	S1-12	1	BB57689_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57689	Sample	BB57689	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-12	IGS	45	BB57689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-12	CGTAATCCTCAGTACTGGCTA	138.5104
BB57689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8371	WGS84	BB57689	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57689_Sspa_Oph2007_COI	S1-12	1	BB57689_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57689	Sample	BB57689	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-12	COI	45	BB57689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-12	GAAGGCCTTCCGATGATC	138.5104
BB57689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8371	WGS84	BB57689	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57689_Upin_Bot2011_COI	S1-12	1	BB57689_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57689	Sample	BB57689	targeted	Absent	Sample	Upin_Bot2011_COI	S1-12	COI	45	BB57689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.5104
BB57690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8365	WGS84	BB57690	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57690_Cmae_Bot2011_COI	S1-14	1	BB57690_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57690	Sample	BB57690	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-14	COI	45	BB57690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-14	ATGAACAGTCTATCCTCCTTTAG	138.50875
BB57692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81094	WGS84	BB57692	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57692_Aamu_Bax2006_COI	S1-19	1	BB57692_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57692	Sample	BB57692	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-19	COI	45	BB57692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-19	GCACAACCGGGATCTTTACTTC	138.52211
BB57692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81094	WGS84	BB57692	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57692_Pcan_Bot2011_IGS	S1-19	1	BB57692_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57692	Sample	BB57692	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-19	IGS	45	BB57692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-19	CGTAATCCTCAGTACTGGCTA	138.52211
BB57692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81094	WGS84	BB57692	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57692_Sspa_Oph2007_COI	S1-19	1	BB57692_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57692	Sample	BB57692	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-19	COI	45	BB57692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-19	GAAGGCCTTCCGATGATC	138.52211
BB57692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81094	WGS84	BB57692	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57692_Upin_Bot2011_COI	S1-19	1	BB57692_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57692	Sample	BB57692	targeted	Absent	Sample	Upin_Bot2011_COI	S1-19	COI	45	BB57692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.52211
BB57693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77643	WGS84	BB57693	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57693_Cjap_Sim2018_COI	S1-01	1	BB57693_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57693	Sample	BB57693	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-01	COI	45	BB57693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51619
BB57693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.77643	WGS84	BB57693	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.884	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57693_Mgig_Bot2012_COI	S1-01	1	BB57693_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57693	Sample	BB57693	targeted	Present	Sample	Mgig_Bot2012_COI	S1-01	COI	45	BB57693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.51619
BB57694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7783	WGS84	BB57694	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57694_Cmae_Bot2011_COI	S1-03	1	BB57694_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57694	Sample	BB57694	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-03	COI	45	BB57694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-03	ATGAACAGTCTATCCTCCTTTAG	138.51642
BB57696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84503	WGS84	BB57696	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57696_Aamu_Bax2006_COI	S1-06	1	BB57696_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57696	Sample	BB57696	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-06	COI	45	BB57696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-06	GCACAACCGGGATCTTTACTTC	138.49721
BB57696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84503	WGS84	BB57696	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57696_Pcan_Bot2011_IGS	S1-06	1	BB57696_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57696	Sample	BB57696	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-06	IGS	45	BB57696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-06	CGTAATCCTCAGTACTGGCTA	138.49721
BB57696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84503	WGS84	BB57696	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57696_Sspa_Oph2007_COI	S1-06	1	BB57696_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57696	Sample	BB57696	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-06	COI	45	BB57696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-06	GAAGGCCTTCCGATGATC	138.49721
BB57696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84503	WGS84	BB57696	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57696_Upin_Bot2011_COI	S1-06	1	BB57696_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57696	Sample	BB57696	targeted	Absent	Sample	Upin_Bot2011_COI	S1-06	COI	45	BB57696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.49721
BB57697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84714	WGS84	BB57697	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57697_Cjap_Sim2018_COI	S1-08	1	BB57697_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57697	Sample	BB57697	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-08	COI	45	BB57697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49732
BB57697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84714	WGS84	BB57697	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.894	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57697_Mgig_Bot2012_COI	S1-08	1	BB57697_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57697	Sample	BB57697	targeted	Present	Sample	Mgig_Bot2012_COI	S1-08	COI	45	BB57697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.49732
BB57698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84408	WGS84	BB57698	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57698_Cmae_Bot2011_COI	S1-10	1	BB57698_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57698	Sample	BB57698	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-10	COI	45	BB57698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-10	ATGAACAGTCTATCCTCCTTTAG	138.49724
BB57700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83672	WGS84	BB57700	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57700_Aamu_Bax2006_COI	S1-13	1	BB57700_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57700	Sample	BB57700	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-13	COI	45	BB57700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-13	GCACAACCGGGATCTTTACTTC	138.50894
BB57700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83672	WGS84	BB57700	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57700_Pcan_Bot2011_IGS	S1-13	1	BB57700_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57700	Sample	BB57700	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-13	IGS	45	BB57700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-13	CGTAATCCTCAGTACTGGCTA	138.50894
BB57700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83672	WGS84	BB57700	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57700_Sspa_Oph2007_COI	S1-13	1	BB57700_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57700	Sample	BB57700	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-13	COI	45	BB57700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-13	GAAGGCCTTCCGATGATC	138.50894
BB57700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83672	WGS84	BB57700	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57700_Upin_Bot2011_COI	S1-13	1	BB57700_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57700	Sample	BB57700	targeted	Absent	Sample	Upin_Bot2011_COI	S1-13	COI	45	BB57700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.50894
BB57701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB57701	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57701_Cjap_Sim2018_COI	S1-15	1	BB57701_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57701	Sample	BB57701	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-15	COI	45	BB57701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50926
BB57701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83674	WGS84	BB57701	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.04	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57701_Mgig_Bot2012_COI	S1-15	1	BB57701_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57701	Sample	BB57701	targeted	Present	Sample	Mgig_Bot2012_COI	S1-15	COI	45	BB57701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.50926
BB57702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80916	WGS84	BB57702	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57702_Cmae_Bot2011_COI	S1-16	1	BB57702_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57702	Sample	BB57702	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-16	COI	45	BB57702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-16	ATGAACAGTCTATCCTCCTTTAG	138.51899
BB57704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82663	WGS84	BB57704	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57704_Aamu_Bax2006_COI	T2-05	1	BB57704_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57704	Sample	BB57704	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-05	COI	45	BB57704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-05	GCACAACCGGGATCTTTACTTC	147.3027
BB57704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82663	WGS84	BB57704	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57704_Pcan_Bot2011_IGS	T2-05	1	BB57704_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57704	Sample	BB57704	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-05	IGS	45	BB57704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-05	CGTAATCCTCAGTACTGGCTA	147.3027
BB57704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82663	WGS84	BB57704	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57704_Sspa_Oph2007_COI	T2-05	1	BB57704_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57704	Sample	BB57704	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-05	COI	45	BB57704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-05	GAAGGCCTTCCGATGATC	147.3027
BB57704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82663	WGS84	BB57704	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57704_Upin_Bot2011_COI	T2-05	1	BB57704_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57704	Sample	BB57704	targeted	Absent	Sample	Upin_Bot2011_COI	T2-05	COI	45	BB57704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.3027
BB57705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87703	WGS84	BB57705	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57705_Cjap_Sim2018_COI	T2-13	1	BB57705_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57705	Sample	BB57705	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-13	COI	45	BB57705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34127
BB57705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87703	WGS84	BB57705	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.437	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57705_Mgig_Bot2012_COI	T2-13	1	BB57705_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57705	Sample	BB57705	targeted	Present	Sample	Mgig_Bot2012_COI	T2-13	COI	45	BB57705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.34127
BB57706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88618	WGS84	BB57706	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57706_Cmae_Bot2011_COI	T2-06	1	BB57706_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57706	Sample	BB57706	targeted	Absent	Sample	Cmae_Bot2011_COI	T2-06	COI	45	BB57706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-06	ATGAACAGTCTATCCTCCTTTAG	147.33863
BB57708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9596	WGS84	BB57708	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57708_Aamu_Bax2006_COI	T2-07	1	BB57708_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57708	Sample	BB57708	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-07	COI	45	BB57708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-07	GCACAACCGGGATCTTTACTTC	147.52467
BB57708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9596	WGS84	BB57708	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57708_Pcan_Bot2011_IGS	T2-07	1	BB57708_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57708	Sample	BB57708	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-07	IGS	45	BB57708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-07	CGTAATCCTCAGTACTGGCTA	147.52467
BB57708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9596	WGS84	BB57708	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57708_Sspa_Oph2007_COI	T2-07	1	BB57708_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57708	Sample	BB57708	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-07	COI	45	BB57708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-07	GAAGGCCTTCCGATGATC	147.52467
BB57708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9596	WGS84	BB57708	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57708_Upin_Bot2011_COI	T2-07	1	BB57708_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57708	Sample	BB57708	targeted	Absent	Sample	Upin_Bot2011_COI	T2-07	COI	45	BB57708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.52467
BB57709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96028	WGS84	BB57709	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57709_Cjap_Sim2018_COI	T3-03	1	BB57709_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57709	Sample	BB57709	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-03	COI	45	BB57709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53093
BB57709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96028	WGS84	BB57709	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.273	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	4/04/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57709_Mgig_Bot2012_COI	T3-03	1	BB57709_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57709	Sample	BB57709	targeted	Present	Sample	Mgig_Bot2012_COI	T3-03	COI	45	BB57709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.53093
BB57711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95936	WGS84	BB57711	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.255	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57711_Aamu_Bax2006_COI	T3-05	1	BB57711_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57711	Sample	BB57711	targeted	Present	Sample	Aamu_Bax2006_COI	T3-05	COI	45	BB57711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-05	GCACAACCGGGATCTTTACTTC	147.52541
BB57711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95936	WGS84	BB57711	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57711_Pcan_Bot2011_IGS	T3-05	1	BB57711_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57711	Sample	BB57711	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-05	IGS	45	BB57711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-05	CGTAATCCTCAGTACTGGCTA	147.52541
BB57711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95936	WGS84	BB57711	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57711_Sspa_Oph2007_COI	T3-05	1	BB57711_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57711	Sample	BB57711	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-05	COI	45	BB57711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-05	GAAGGCCTTCCGATGATC	147.52541
BB57711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95936	WGS84	BB57711	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57711_Upin_Bot2011_COI	T3-05	1	BB57711_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57711	Sample	BB57711	targeted	Absent	Sample	Upin_Bot2011_COI	T3-05	COI	45	BB57711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.52541
BB57712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82837	WGS84	BB57712	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57712_Cjap_Sim2018_COI	T2-01	1	BB57712_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57712	Sample	BB57712	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-01	COI	45	BB57712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30181
BB57712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82837	WGS84	BB57712	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.776	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	23/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57712_Mgig_Bot2012_COI	T2-01	1	BB57712_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57712	Sample	BB57712	targeted	Present	Sample	Mgig_Bot2012_COI	T2-01	COI	45	BB57712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.30181
BB57713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82503	WGS84	BB57713	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.607	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57713_Cmae_Bot2011_COI	T2-09	1	BB57713_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57713	Sample	BB57713	targeted	Present	Sample	Cmae_Bot2011_COI	T2-09	COI	45	BB57713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-09	ATGAACAGTCTATCCTCCTTTAG	147.30534
BB57715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88566	WGS84	BB57715	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57715_Aamu_Bax2006_COI	T2-02	1	BB57715_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57715	Sample	BB57715	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-02	COI	45	BB57715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-02	GCACAACCGGGATCTTTACTTC	147.33452
BB57715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88566	WGS84	BB57715	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57715_Pcan_Bot2011_IGS	T2-02	1	BB57715_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57715	Sample	BB57715	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-02	IGS	45	BB57715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-02	CGTAATCCTCAGTACTGGCTA	147.33452
BB57715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88566	WGS84	BB57715	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57715_Sspa_Oph2007_COI	T2-02	1	BB57715_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57715	Sample	BB57715	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-02	COI	45	BB57715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-02	GAAGGCCTTCCGATGATC	147.33452
BB57715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88566	WGS84	BB57715	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57715_Upin_Bot2011_COI	T2-02	1	BB57715_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57715	Sample	BB57715	targeted	Absent	Sample	Upin_Bot2011_COI	T2-02	COI	45	BB57715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.33452
BB57716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88465	WGS84	BB57716	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57716_Cjap_Sim2018_COI	T2-10	1	BB57716_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57716	Sample	BB57716	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-10	COI	45	BB57716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33621
BB57716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88465	WGS84	BB57716	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.067	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57716_Mgig_Bot2012_COI	T2-10	1	BB57716_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57716	Sample	BB57716	targeted	Present	Sample	Mgig_Bot2012_COI	T2-10	COI	45	BB57716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.33621
BB57717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88259	WGS84	BB57717	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.481	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57717_Cmae_Bot2011_COI	T2-16	1	BB57717_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57717	Sample	BB57717	targeted	Present	Sample	Cmae_Bot2011_COI	T2-16	COI	45	BB57717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-16	ATGAACAGTCTATCCTCCTTTAG	147.33612
BB57719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96959	WGS84	BB57719	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.429	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57719_Aamu_Bax2006_COI	T2-11	1	BB57719_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57719	Sample	BB57719	targeted	Present	Sample	Aamu_Bax2006_COI	T2-11	COI	45	BB57719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-11	GCACAACCGGGATCTTTACTTC	147.51602
BB57719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96959	WGS84	BB57719	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57719_Pcan_Bot2011_IGS	T2-11	1	BB57719_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57719	Sample	BB57719	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-11	IGS	45	BB57719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-11	CGTAATCCTCAGTACTGGCTA	147.51602
BB57719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96959	WGS84	BB57719	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57719_Sspa_Oph2007_COI	T2-11	1	BB57719_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57719	Sample	BB57719	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-11	COI	45	BB57719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-11	GAAGGCCTTCCGATGATC	147.51602
BB57719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96959	WGS84	BB57719	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57719_Upin_Bot2011_COI	T2-11	1	BB57719_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57719	Sample	BB57719	targeted	Absent	Sample	Upin_Bot2011_COI	T2-11	COI	45	BB57719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.51602
BB57720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84978	WGS84	BB57720	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57720_Cmae_Bot2011_COI	T3-06	1	BB57720_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57720	Sample	BB57720	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-06	COI	45	BB57720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-06	ATGAACAGTCTATCCTCCTTTAG	147.8733
BB57722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85306	WGS84	BB57722	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57722_Aamu_Bax2006_COI	T2-12	1	BB57722_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57722	Sample	BB57722	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-12	COI	45	BB57722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-12	GCACAACCGGGATCTTTACTTC	147.87211
BB57722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85306	WGS84	BB57722	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57722_Pcan_Bot2011_IGS	T2-12	1	BB57722_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57722	Sample	BB57722	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-12	IGS	45	BB57722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-12	CGTAATCCTCAGTACTGGCTA	147.87211
BB57722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85306	WGS84	BB57722	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57722_Sspa_Oph2007_COI	T2-12	1	BB57722_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57722	Sample	BB57722	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-12	COI	45	BB57722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-12	GAAGGCCTTCCGATGATC	147.87211
BB57722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85306	WGS84	BB57722	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57722_Upin_Bot2011_COI	T2-12	1	BB57722_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57722	Sample	BB57722	targeted	Absent	Sample	Upin_Bot2011_COI	T2-12	COI	45	BB57722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.87211
BB57723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87738	WGS84	BB57723	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57723_Cjap_Sim2018_COI	T3-04	1	BB57723_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57723	Sample	BB57723	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-04	COI	45	BB57723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34147
BB57723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87738	WGS84	BB57723	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.729	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57723_Mgig_Bot2012_COI	T3-04	1	BB57723_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57723	Sample	BB57723	targeted	Present	Sample	Mgig_Bot2012_COI	T3-04	COI	45	BB57723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.34147
BB57724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84683	WGS84	BB57724	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57724_Cmae_Bot2011_COI	S2-01	1	BB57724_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57724	Sample	BB57724	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-01	COI	45	BB57724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-01	ATGAACAGTCTATCCTCCTTTAG	138.49744
BB57726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7866	WGS84	BB57726	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57726_Aamu_Bax2006_COI	S2-12	1	BB57726_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57726	Sample	BB57726	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-12	COI	45	BB57726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-12	GCACAACCGGGATCTTTACTTC	138.51435
BB57726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7866	WGS84	BB57726	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57726_Pcan_Bot2011_IGS	S2-12	1	BB57726_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57726	Sample	BB57726	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-12	IGS	45	BB57726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-12	CGTAATCCTCAGTACTGGCTA	138.51435
BB57726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7866	WGS84	BB57726	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.477	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57726_Sspa_Oph2007_COI	S2-12	1	BB57726_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57726	Sample	BB57726	targeted	Present	Sample	Sspa_Oph2007_COI	S2-12	COI	45	BB57726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-12	GAAGGCCTTCCGATGATC	138.51435
BB57726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7866	WGS84	BB57726	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57726_Upin_Bot2011_COI	S2-12	1	BB57726_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57726	Sample	BB57726	targeted	Absent	Sample	Upin_Bot2011_COI	S2-12	COI	45	BB57726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.51435
BB57727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83676	WGS84	BB57727	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57727_Cjap_Sim2018_COI	S2-09	1	BB57727_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57727	Sample	BB57727	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-09	COI	45	BB57727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50937
BB57727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83676	WGS84	BB57727	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57727_Mgig_Bot2012_COI	S2-09	1	BB57727_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57727	Sample	BB57727	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-09	COI	45	BB57727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.50937
BB57728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83658	WGS84	BB57728	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57728_Cmae_Bot2011_COI	S2-07	1	BB57728_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57728	Sample	BB57728	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-07	COI	45	BB57728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-07	ATGAACAGTCTATCCTCCTTTAG	138.50823
BB57730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84664	WGS84	BB57730	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57730_Cjap_Sim2018_COI	S2-02	1	BB57730_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57730	Sample	BB57730	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-02	COI	45	BB57730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49722
BB57730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84664	WGS84	BB57730	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57730_Mgig_Bot2012_COI	S2-02	1	BB57730_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57730	Sample	BB57730	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-02	COI	45	BB57730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.49722
BB57731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82563	WGS84	BB57731	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57731_Cmae_Bot2011_COI	T3-09	1	BB57731_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57731	Sample	BB57731	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-09	COI	45	BB57731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-09	ATGAACAGTCTATCCTCCTTTAG	147.30458
BB57733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82744	WGS84	BB57733	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57733_Aamu_Bax2006_COI	T3-08	1	BB57733_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57733	Sample	BB57733	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-08	COI	45	BB57733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-08	GCACAACCGGGATCTTTACTTC	147.30218
BB57733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82744	WGS84	BB57733	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57733_Pcan_Bot2011_IGS	T3-08	1	BB57733_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57733	Sample	BB57733	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-08	IGS	45	BB57733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-08	CGTAATCCTCAGTACTGGCTA	147.30218
BB57733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82744	WGS84	BB57733	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57733_Sspa_Oph2007_COI	T3-08	1	BB57733_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57733	Sample	BB57733	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-08	COI	45	BB57733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-08	GAAGGCCTTCCGATGATC	147.30218
BB57733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82744	WGS84	BB57733	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57733_Upin_Bot2011_COI	T3-08	1	BB57733_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57733	Sample	BB57733	targeted	Absent	Sample	Upin_Bot2011_COI	T3-08	COI	45	BB57733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.30218
BB57734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85563	WGS84	BB57734	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57734_Cjap_Sim2018_COI	T3-12	1	BB57734_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57734	Sample	BB57734	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-12	COI	45	BB57734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87212
BB57734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85563	WGS84	BB57734	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.352	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	4/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57734_Mgig_Bot2012_COI	T3-12	1	BB57734_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57734	Sample	BB57734	targeted	Present	Sample	Mgig_Bot2012_COI	T3-12	COI	45	BB57734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.87212
BB57735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88567	WGS84	BB57735	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.425	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57735_Cmae_Bot2011_COI	T3-02	1	BB57735_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57735	Sample	BB57735	targeted	Present	Sample	Cmae_Bot2011_COI	T3-02	COI	45	BB57735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-02	ATGAACAGTCTATCCTCCTTTAG	147.33476
BB57737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88386	WGS84	BB57737	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57737_Aamu_Bax2006_COI	T3-11	1	BB57737_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57737	Sample	BB57737	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-11	COI	45	BB57737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-11	GCACAACCGGGATCTTTACTTC	147.33667
BB57737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88386	WGS84	BB57737	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57737_Pcan_Bot2011_IGS	T3-11	1	BB57737_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57737	Sample	BB57737	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-11	IGS	45	BB57737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-11	CGTAATCCTCAGTACTGGCTA	147.33667
BB57737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88386	WGS84	BB57737	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57737_Sspa_Oph2007_COI	T3-11	1	BB57737_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57737	Sample	BB57737	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-11	COI	45	BB57737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-11	GAAGGCCTTCCGATGATC	147.33667
BB57737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88386	WGS84	BB57737	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57737_Upin_Bot2011_COI	T3-11	1	BB57737_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57737	Sample	BB57737	targeted	Absent	Sample	Upin_Bot2011_COI	T3-11	COI	45	BB57737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33667
BB57738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88476	WGS84	BB57738	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57738_Cjap_Sim2018_COI	T3-17	1	BB57738_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57738	Sample	BB57738	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-17	COI	45	BB57738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33551
BB57738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88476	WGS84	BB57738	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.472	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57738_Mgig_Bot2012_COI	T3-17	1	BB57738_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57738	Sample	BB57738	targeted	Present	Sample	Mgig_Bot2012_COI	T3-17	COI	45	BB57738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-17	TCTTATTCGTTGGAGACTTTATAACCCT	147.33551
BB57740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96226	WGS84	BB57740	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57740_Aamu_Bax2006_COI	T3-10	1	BB57740_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57740	Sample	BB57740	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-10	COI	45	BB57740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-10	GCACAACCGGGATCTTTACTTC	147.51947
BB57740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96226	WGS84	BB57740	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57740_Pcan_Bot2011_IGS	T3-10	1	BB57740_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57740	Sample	BB57740	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-10	IGS	45	BB57740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-10	CGTAATCCTCAGTACTGGCTA	147.51947
BB57740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96226	WGS84	BB57740	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57740_Sspa_Oph2007_COI	T3-10	1	BB57740_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57740	Sample	BB57740	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-10	COI	45	BB57740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-10	GAAGGCCTTCCGATGATC	147.51947
BB57740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96226	WGS84	BB57740	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.108	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57740_Upin_Bot2011_COI	T3-10	1	BB57740_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57740	Sample	BB57740	targeted	Present	Sample	Upin_Bot2011_COI	T3-10	COI	45	BB57740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.51947
BB57741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88326	WGS84	BB57741	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57741_Cjap_Sim2018_COI	T3-16	1	BB57741_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57741	Sample	BB57741	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-16	COI	45	BB57741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33803
BB57741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88326	WGS84	BB57741	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.307	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57741_Mgig_Bot2012_COI	T3-16	1	BB57741_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57741	Sample	BB57741	targeted	Present	Sample	Mgig_Bot2012_COI	T3-16	COI	45	BB57741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.33803
BB57742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88955	WGS84	BB57742	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57742_Cmae_Bot2011_COI	T3-13	1	BB57742_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57742	Sample	BB57742	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-13	COI	45	BB57742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-13	ATGAACAGTCTATCCTCCTTTAG	147.81318
BB57744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87892	WGS84	BB57744	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57744_Aamu_Bax2006_COI	T3-14	1	BB57744_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57744	Sample	BB57744	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-14	COI	45	BB57744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-14	GCACAACCGGGATCTTTACTTC	147.34139
BB57744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87892	WGS84	BB57744	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57744_Pcan_Bot2011_IGS	T3-14	1	BB57744_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57744	Sample	BB57744	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-14	IGS	45	BB57744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-14	CGTAATCCTCAGTACTGGCTA	147.34139
BB57744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87892	WGS84	BB57744	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57744_Sspa_Oph2007_COI	T3-14	1	BB57744_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57744	Sample	BB57744	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-14	COI	45	BB57744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-14	GAAGGCCTTCCGATGATC	147.34139
BB57744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87892	WGS84	BB57744	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.111	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57744_Upin_Bot2011_COI	T3-14	1	BB57744_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57744	Sample	BB57744	targeted	Present	Sample	Upin_Bot2011_COI	T3-14	COI	45	BB57744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.34139
BB73901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82045	WGS84	BB73901	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73901_Mgig_Bot2012_COI	MA51	1	BB73901_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73901	Sample	BB73901	targeted	Absent	Sample	Mgig_Bot2012_COI	MA51	COI	45	BB73901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA51	TCTTATTCGTTGGAGACTTTATAACCCT	144.93041
BB73902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82086	WGS84	BB73902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73902_Cmae_Bot2011_COI	MA49	1	BB73902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73902	Sample	BB73902	targeted	Absent	Sample	Cmae_Bot2011_COI	MA49	COI	45	BB73902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA49	ATGAACAGTCTATCCTCCTTTAG	144.91451
BB73902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82086	WGS84	BB73902	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73902_Msal_Bot2012_COI	MA49	1	BB73902_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73902	Sample	BB73902	targeted	Absent	Sample	Msal_Bot2012_COI	MA49	COI	45	BB73902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA49	GYTAGTTCCRATGATGTTAGCTG	144.91451
BB73904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82918	WGS84	BB73904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.301	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73904_Aamu_Bax2006_COI	MA47	1	BB73904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73904	Sample	BB73904	targeted	Present	Sample	Aamu_Bax2006_COI	MA47	COI	45	BB73904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA47	GCACAACCGGGATCTTTACTTC	144.90038
BB73904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82918	WGS84	BB73904	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73904_Pcan_Bot2011_IGS	MA47	1	BB73904_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73904	Sample	BB73904	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA47	IGS	45	BB73904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA47	CGTAATCCTCAGTACTGGCTA	144.90038
BB73904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82918	WGS84	BB73904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73904_Sspa_Oph2007_COI	MA47	1	BB73904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73904	Sample	BB73904	targeted	Absent	Sample	Sspa_Oph2007_COI	MA47	COI	45	BB73904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA47	GAAGGCCTTCCGATGATC	144.90038
BB73904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82918	WGS84	BB73904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73904_Upin_Bot2011_COI	MA47	1	BB73904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73904	Sample	BB73904	targeted	Absent	Sample	Upin_Bot2011_COI	MA47	COI	45	BB73904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA47	CTTTAATTACAGCGTTTTTATTGTTGT	144.90038
BB73905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.35748	WGS84	BB73905	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73905_Mgig_Bot2012_COI	BA20	1	BB73905_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73905	Sample	BB73905	targeted	Absent	Sample	Mgig_Bot2012_COI	BA20	COI	45	BB73905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA20	TCTTATTCGTTGGAGACTTTATAACCCT	153.18045
BB73906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82042	WGS84	BB73906	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73906_Cmae_Bot2011_COI	MA17	1	BB73906_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73906	Sample	BB73906	targeted	Absent	Sample	Cmae_Bot2011_COI	MA17	COI	45	BB73906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA17	ATGAACAGTCTATCCTCCTTTAG	144.91668
BB73906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82042	WGS84	BB73906	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73906_Msal_Bot2012_COI	MA17	1	BB73906_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73906	Sample	BB73906	targeted	Absent	Sample	Msal_Bot2012_COI	MA17	COI	45	BB73906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA17	GYTAGTTCCRATGATGTTAGCTG	144.91668
BB73908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81914	WGS84	BB73908	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.413	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73908_Aamu_Bax2006_COI	MA6	1	BB73908_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73908	Sample	BB73908	targeted	Present	Sample	Aamu_Bax2006_COI	MA6	COI	45	BB73908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA6	GCACAACCGGGATCTTTACTTC	144.92582
BB73908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81914	WGS84	BB73908	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73908_Pcan_Bot2011_IGS	MA6	1	BB73908_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73908	Sample	BB73908	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA6	IGS	45	BB73908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA6	CGTAATCCTCAGTACTGGCTA	144.92582
BB73908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81914	WGS84	BB73908	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.27	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73908_Sspa_Oph2007_COI	MA6	1	BB73908_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73908	Sample	BB73908	targeted	Present	Sample	Sspa_Oph2007_COI	MA6	COI	45	BB73908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA6	GAAGGCCTTCCGATGATC	144.92582
BB73908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81914	WGS84	BB73908	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73908_Upin_Bot2011_COI	MA6	1	BB73908_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73908	Sample	BB73908	targeted	Absent	Sample	Upin_Bot2011_COI	MA6	COI	45	BB73908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA6	CTTTAATTACAGCGTTTTTATTGTTGT	144.92582
BB73909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.4463	WGS84	BB73909	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73909_Mgig_Bot2012_COI	BA6	1	BB73909_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73909	Sample	BB73909	targeted	Absent	Sample	Mgig_Bot2012_COI	BA6	COI	45	BB73909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA6	TCTTATTCGTTGGAGACTTTATAACCCT	153.07695
BB73911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB73911	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.441	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73911_Aamu_Bax2006_COI	MA2	1	BB73911_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73911	Sample	BB73911	targeted	Present	Sample	Aamu_Bax2006_COI	MA2	COI	45	BB73911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA2	GCACAACCGGGATCTTTACTTC	144.93107
BB73911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB73911	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73911_Pcan_Bot2011_IGS	MA2	1	BB73911_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73911	Sample	BB73911	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA2	IGS	45	BB73911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA2	CGTAATCCTCAGTACTGGCTA	144.93107
BB73911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB73911	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73911_Sspa_Oph2007_COI	MA2	1	BB73911_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73911	Sample	BB73911	targeted	Absent	Sample	Sspa_Oph2007_COI	MA2	COI	45	BB73911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA2	GAAGGCCTTCCGATGATC	144.93107
BB73911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB73911	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73911_Upin_Bot2011_COI	MA2	1	BB73911_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73911	Sample	BB73911	targeted	Absent	Sample	Upin_Bot2011_COI	MA2	COI	45	BB73911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA2	CTTTAATTACAGCGTTTTTATTGTTGT	144.93107
BB73912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81865	WGS84	BB73912	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73912_Mgig_Bot2012_COI	MA1	1	BB73912_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73912	Sample	BB73912	targeted	Absent	Sample	Mgig_Bot2012_COI	MA1	COI	45	BB73912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA1	TCTTATTCGTTGGAGACTTTATAACCCT	144.9245
BB73913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81128	WGS84	BB73913	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73913_Cmae_Bot2011_COI	GA110	1	BB73913_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73913	Sample	BB73913	targeted	Absent	Sample	Cmae_Bot2011_COI	GA110	COI	45	BB73913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA110	ATGAACAGTCTATCCTCCTTTAG	144.91423
BB73913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81128	WGS84	BB73913	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73913_Msal_Bot2012_COI	GA110	1	BB73913_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73913	Sample	BB73913	targeted	Absent	Sample	Msal_Bot2012_COI	GA110	COI	45	BB73913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA110	GYTAGTTCCRATGATGTTAGCTG	144.91423
BB73915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82059	WGS84	BB73915	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.14	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73915_Aamu_Bax2006_COI	GA100	1	BB73915_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73915	Sample	BB73915	targeted	Present	Sample	Aamu_Bax2006_COI	GA100	COI	45	BB73915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA100	GCACAACCGGGATCTTTACTTC	144.90597
BB73915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82059	WGS84	BB73915	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73915_Pcan_Bot2011_IGS	GA100	1	BB73915_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73915	Sample	BB73915	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA100	IGS	45	BB73915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA100	CGTAATCCTCAGTACTGGCTA	144.90597
BB73915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82059	WGS84	BB73915	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73915_Sspa_Oph2007_COI	GA100	1	BB73915_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73915	Sample	BB73915	targeted	Absent	Sample	Sspa_Oph2007_COI	GA100	COI	45	BB73915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA100	GAAGGCCTTCCGATGATC	144.90597
BB73915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82059	WGS84	BB73915	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73915_Upin_Bot2011_COI	GA100	1	BB73915_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73915	Sample	BB73915	targeted	Absent	Sample	Upin_Bot2011_COI	GA100	COI	45	BB73915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA100	CTTTAATTACAGCGTTTTTATTGTTGT	144.90597
BB73916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82708	WGS84	BB73916	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73916_Mgig_Bot2012_COI	GA95	1	BB73916_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73916	Sample	BB73916	targeted	Absent	Sample	Mgig_Bot2012_COI	GA95	COI	45	BB73916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA95	TCTTATTCGTTGGAGACTTTATAACCCT	144.89976
BB73917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81858	WGS84	BB73917	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73917_Cmae_Bot2011_COI	GA90	1	BB73917_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73917	Sample	BB73917	targeted	Absent	Sample	Cmae_Bot2011_COI	GA90	COI	45	BB73917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA90	ATGAACAGTCTATCCTCCTTTAG	144.9152
BB73917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81858	WGS84	BB73917	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73917_Msal_Bot2012_COI	GA90	1	BB73917_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73917	Sample	BB73917	targeted	Absent	Sample	Msal_Bot2012_COI	GA90	COI	45	BB73917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA90	GYTAGTTCCRATGATGTTAGCTG	144.9152
BB73919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81129	WGS84	BB73919	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.4	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73919_Aamu_Bax2006_COI	GA80	1	BB73919_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73919	Sample	BB73919	targeted	Present	Sample	Aamu_Bax2006_COI	GA80	COI	45	BB73919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA80	GCACAACCGGGATCTTTACTTC	144.91354
BB73919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81129	WGS84	BB73919	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73919_Pcan_Bot2011_IGS	GA80	1	BB73919_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73919	Sample	BB73919	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA80	IGS	45	BB73919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA80	CGTAATCCTCAGTACTGGCTA	144.91354
BB73919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81129	WGS84	BB73919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73919_Sspa_Oph2007_COI	GA80	1	BB73919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73919	Sample	BB73919	targeted	Absent	Sample	Sspa_Oph2007_COI	GA80	COI	45	BB73919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA80	GAAGGCCTTCCGATGATC	144.91354
BB73919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81129	WGS84	BB73919	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73919_Upin_Bot2011_COI	GA80	1	BB73919_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73919	Sample	BB73919	targeted	Absent	Sample	Upin_Bot2011_COI	GA80	COI	45	BB73919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA80	CTTTAATTACAGCGTTTTTATTGTTGT	144.91354
BB73920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81899	WGS84	BB73920	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73920_Cmae_Bot2011_COI	GA75	1	BB73920_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73920	Sample	BB73920	targeted	Absent	Sample	Cmae_Bot2011_COI	GA75	COI	45	BB73920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA75	ATGAACAGTCTATCCTCCTTTAG	144.91136
BB73920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81899	WGS84	BB73920	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73920_Msal_Bot2012_COI	GA75	1	BB73920_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73920	Sample	BB73920	targeted	Absent	Sample	Msal_Bot2012_COI	GA75	COI	45	BB73920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA75	GYTAGTTCCRATGATGTTAGCTG	144.91136
BB73922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82287	WGS84	BB73922	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.265	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73922_Aamu_Bax2006_COI	GA65	1	BB73922_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73922	Sample	BB73922	targeted	Present	Sample	Aamu_Bax2006_COI	GA65	COI	45	BB73922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA65	GCACAACCGGGATCTTTACTTC	144.90529
BB73922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82287	WGS84	BB73922	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73922_Pcan_Bot2011_IGS	GA65	1	BB73922_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73922	Sample	BB73922	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA65	IGS	45	BB73922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA65	CGTAATCCTCAGTACTGGCTA	144.90529
BB73922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82287	WGS84	BB73922	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	42.676	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73922_Sspa_Oph2007_COI	GA65	1	BB73922_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73922	Sample	BB73922	targeted	Present	Sample	Sspa_Oph2007_COI	GA65	COI	45	BB73922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA65	GAAGGCCTTCCGATGATC	144.90529
BB73922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82287	WGS84	BB73922	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73922_Upin_Bot2011_COI	GA65	1	BB73922_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73922	Sample	BB73922	targeted	Absent	Sample	Upin_Bot2011_COI	GA65	COI	45	BB73922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA65	CTTTAATTACAGCGTTTTTATTGTTGT	144.90529
BB73923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44494	WGS84	BB73923	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73923_Mgig_Bot2012_COI	BA13	1	BB73923_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73923	Sample	BB73923	targeted	Absent	Sample	Mgig_Bot2012_COI	BA13	COI	45	BB73923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA13	TCTTATTCGTTGGAGACTTTATAACCCT	153.08106
BB73924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81631	WGS84	BB73924	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73924_Cmae_Bot2011_COI	GA50	1	BB73924_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73924	Sample	BB73924	targeted	Absent	Sample	Cmae_Bot2011_COI	GA50	COI	45	BB73924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA50	ATGAACAGTCTATCCTCCTTTAG	144.91317
BB73924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81631	WGS84	BB73924	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73924_Msal_Bot2012_COI	GA50	1	BB73924_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73924	Sample	BB73924	targeted	Absent	Sample	Msal_Bot2012_COI	GA50	COI	45	BB73924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA50	GYTAGTTCCRATGATGTTAGCTG	144.91317
BB73926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44611	WGS84	BB73926	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73926_Aamu_Bax2006_COI	BA14	1	BB73926_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73926	Sample	BB73926	targeted	Absent	Sample	Aamu_Bax2006_COI	BA14	COI	45	BB73926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA14	GCACAACCGGGATCTTTACTTC	153.08337
BB73926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44611	WGS84	BB73926	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73926_Pcan_Bot2011_IGS	BA14	1	BB73926_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73926	Sample	BB73926	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA14	IGS	45	BB73926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA14	CGTAATCCTCAGTACTGGCTA	153.08337
BB73926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44611	WGS84	BB73926	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73926_Sspa_Oph2007_COI	BA14	1	BB73926_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73926	Sample	BB73926	targeted	Absent	Sample	Sspa_Oph2007_COI	BA14	COI	45	BB73926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA14	GAAGGCCTTCCGATGATC	153.08337
BB73926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44611	WGS84	BB73926	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73926_Upin_Bot2011_COI	BA14	1	BB73926_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73926	Sample	BB73926	targeted	Absent	Sample	Upin_Bot2011_COI	BA14	COI	45	BB73926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA14	CTTTAATTACAGCGTTTTTATTGTTGT	153.08337
BB73927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81859	WGS84	BB73927	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73927_Mgig_Bot2012_COI	GA35	1	BB73927_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73927	Sample	BB73927	targeted	Absent	Sample	Mgig_Bot2012_COI	GA35	COI	45	BB73927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA35	TCTTATTCGTTGGAGACTTTATAACCCT	144.90601
BB73928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81867	WGS84	BB73928	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73928_Cmae_Bot2011_COI	GA30	1	BB73928_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73928	Sample	BB73928	targeted	Absent	Sample	Cmae_Bot2011_COI	GA30	COI	45	BB73928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA30	ATGAACAGTCTATCCTCCTTTAG	144.91782
BB73928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81867	WGS84	BB73928	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73928_Msal_Bot2012_COI	GA30	1	BB73928_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73928	Sample	BB73928	targeted	Absent	Sample	Msal_Bot2012_COI	GA30	COI	45	BB73928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA30	GYTAGTTCCRATGATGTTAGCTG	144.91782
BB73930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37364	WGS84	BB73930	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73930_Mgig_Bot2012_COI	BA30	1	BB73930_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73930	Sample	BB73930	targeted	Absent	Sample	Mgig_Bot2012_COI	BA30	COI	45	BB73930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA30	TCTTATTCGTTGGAGACTTTATAACCCT	153.16922
BB73931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44806	WGS84	BB73931	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73931_Cmae_Bot2011_COI	BA17	1	BB73931_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73931	Sample	BB73931	targeted	Absent	Sample	Cmae_Bot2011_COI	BA17	COI	45	BB73931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA17	ATGAACAGTCTATCCTCCTTTAG	153.094
BB73931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44806	WGS84	BB73931	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73931_Msal_Bot2012_COI	BA17	1	BB73931_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73931	Sample	BB73931	targeted	Absent	Sample	Msal_Bot2012_COI	BA17	COI	45	BB73931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA17	GYTAGTTCCRATGATGTTAGCTG	153.094
BB73933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43303	WGS84	BB73933	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73933_Aamu_Bax2006_COI	BA18	1	BB73933_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73933	Sample	BB73933	targeted	Absent	Sample	Aamu_Bax2006_COI	BA18	COI	45	BB73933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA18	GCACAACCGGGATCTTTACTTC	153.11629
BB73933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43303	WGS84	BB73933	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73933_Pcan_Bot2011_IGS	BA18	1	BB73933_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73933	Sample	BB73933	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA18	IGS	45	BB73933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA18	CGTAATCCTCAGTACTGGCTA	153.11629
BB73933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43303	WGS84	BB73933	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73933_Sspa_Oph2007_COI	BA18	1	BB73933_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73933	Sample	BB73933	targeted	Absent	Sample	Sspa_Oph2007_COI	BA18	COI	45	BB73933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA18	GAAGGCCTTCCGATGATC	153.11629
BB73933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43303	WGS84	BB73933	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73933_Upin_Bot2011_COI	BA18	1	BB73933_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73933	Sample	BB73933	targeted	Absent	Sample	Upin_Bot2011_COI	BA18	COI	45	BB73933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA18	CTTTAATTACAGCGTTTTTATTGTTGT	153.11629
BB73934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44723	WGS84	BB73934	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73934_Mgig_Bot2012_COI	BA15	1	BB73934_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73934	Sample	BB73934	targeted	Absent	Sample	Mgig_Bot2012_COI	BA15	COI	45	BB73934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA15	TCTTATTCGTTGGAGACTTTATAACCCT	153.09789
BB73935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85877	WGS84	BB73935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.351	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73935_Cmae_Bot2011_COI	MA119	1	BB73935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73935	Sample	BB73935	targeted	Present	Sample	Cmae_Bot2011_COI	MA119	COI	45	BB73935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA119	ATGAACAGTCTATCCTCCTTTAG	144.91141
BB73935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85877	WGS84	BB73935	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73935_Msal_Bot2012_COI	MA119	1	BB73935_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73935	Sample	BB73935	targeted	Absent	Sample	Msal_Bot2012_COI	MA119	COI	45	BB73935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA119	GYTAGTTCCRATGATGTTAGCTG	144.91141
BB73937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86045	WGS84	BB73937	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.918	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73937_Aamu_Bax2006_COI	MA87	1	BB73937_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73937	Sample	BB73937	targeted	Present	Sample	Aamu_Bax2006_COI	MA87	COI	45	BB73937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA87	GCACAACCGGGATCTTTACTTC	144.91439
BB73937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86045	WGS84	BB73937	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73937_Pcan_Bot2011_IGS	MA87	1	BB73937_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73937	Sample	BB73937	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA87	IGS	45	BB73937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA87	CGTAATCCTCAGTACTGGCTA	144.91439
BB73937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86045	WGS84	BB73937	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.624	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73937_Sspa_Oph2007_COI	MA87	1	BB73937_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73937	Sample	BB73937	targeted	Present	Sample	Sspa_Oph2007_COI	MA87	COI	45	BB73937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA87	GAAGGCCTTCCGATGATC	144.91439
BB73937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86045	WGS84	BB73937	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.152	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73937_Upin_Bot2011_COI	MA87	1	BB73937_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73937	Sample	BB73937	targeted	Present	Sample	Upin_Bot2011_COI	MA87	COI	45	BB73937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA87	CTTTAATTACAGCGTTTTTATTGTTGT	144.91439
BB73938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36254	WGS84	BB73938	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73938_Mgig_Bot2012_COI	BA24	1	BB73938_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73938	Sample	BB73938	targeted	Absent	Sample	Mgig_Bot2012_COI	BA24	COI	45	BB73938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA24	TCTTATTCGTTGGAGACTTTATAACCCT	153.17555
BB73939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36686	WGS84	BB73939	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73939_Cmae_Bot2011_COI	BA26	1	BB73939_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73939	Sample	BB73939	targeted	Absent	Sample	Cmae_Bot2011_COI	BA26	COI	45	BB73939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA26	ATGAACAGTCTATCCTCCTTTAG	153.17334
BB73939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36686	WGS84	BB73939	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73939_Msal_Bot2012_COI	BA26	1	BB73939_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73939	Sample	BB73939	targeted	Absent	Sample	Msal_Bot2012_COI	BA26	COI	45	BB73939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA26	GYTAGTTCCRATGATGTTAGCTG	153.17334
BB73940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3702	WGS84	BB73940	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73940_Aamu_Bax2006_COI	BA28	1	BB73940_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73940	Sample	BB73940	targeted	Absent	Sample	Aamu_Bax2006_COI	BA28	COI	45	BB73940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA28	GCACAACCGGGATCTTTACTTC	153.1708
BB73940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3702	WGS84	BB73940	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73940_Pcan_Bot2011_IGS	BA28	1	BB73940_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73940	Sample	BB73940	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA28	IGS	45	BB73940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA28	CGTAATCCTCAGTACTGGCTA	153.1708
BB73940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3702	WGS84	BB73940	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73940_Sspa_Oph2007_COI	BA28	1	BB73940_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73940	Sample	BB73940	targeted	Absent	Sample	Sspa_Oph2007_COI	BA28	COI	45	BB73940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA28	GAAGGCCTTCCGATGATC	153.1708
BB73940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3702	WGS84	BB73940	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73940_Upin_Bot2011_COI	BA28	1	BB73940_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73940	Sample	BB73940	targeted	Absent	Sample	Upin_Bot2011_COI	BA28	COI	45	BB73940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA28	CTTTAATTACAGCGTTTTTATTGTTGT	153.1708
BB73941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86508	WGS84	BB73941	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73941_Mgig_Bot2012_COI	MA81	1	BB73941_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73941	Sample	BB73941	targeted	Absent	Sample	Mgig_Bot2012_COI	MA81	COI	45	BB73941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA81	TCTTATTCGTTGGAGACTTTATAACCCT	144.91763
BB73942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86185	WGS84	BB73942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73942_Cmae_Bot2011_COI	MA79	1	BB73942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73942	Sample	BB73942	targeted	Absent	Sample	Cmae_Bot2011_COI	MA79	COI	45	BB73942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA79	ATGAACAGTCTATCCTCCTTTAG	144.91458
BB73942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86185	WGS84	BB73942	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73942_Msal_Bot2012_COI	MA79	1	BB73942_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73942	Sample	BB73942	targeted	Absent	Sample	Msal_Bot2012_COI	MA79	COI	45	BB73942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA79	GYTAGTTCCRATGATGTTAGCTG	144.91458
BB73944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86423	WGS84	BB73944	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.011	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73944_Aamu_Bax2006_COI	MA76	1	BB73944_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73944	Sample	BB73944	targeted	Present	Sample	Aamu_Bax2006_COI	MA76	COI	45	BB73944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA76	GCACAACCGGGATCTTTACTTC	144.91825
BB73944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86423	WGS84	BB73944	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73944_Pcan_Bot2011_IGS	MA76	1	BB73944_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73944	Sample	BB73944	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA76	IGS	45	BB73944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA76	CGTAATCCTCAGTACTGGCTA	144.91825
BB73944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86423	WGS84	BB73944	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73944_Sspa_Oph2007_COI	MA76	1	BB73944_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73944	Sample	BB73944	targeted	Absent	Sample	Sspa_Oph2007_COI	MA76	COI	45	BB73944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA76	GAAGGCCTTCCGATGATC	144.91825
BB73944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86423	WGS84	BB73944	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.258	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73944_Upin_Bot2011_COI	MA76	1	BB73944_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73944	Sample	BB73944	targeted	Present	Sample	Upin_Bot2011_COI	MA76	COI	45	BB73944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA76	CTTTAATTACAGCGTTTTTATTGTTGT	144.91825
BB73945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43165	WGS84	BB73945	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73945_Mgig_Bot2012_COI	BA65	1	BB73945_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73945	Sample	BB73945	targeted	Absent	Sample	Mgig_Bot2012_COI	BA65	COI	45	BB73945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA65	TCTTATTCGTTGGAGACTTTATAACCCT	153.12161
BB73946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37653	WGS84	BB73946	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73946_Cmae_Bot2011_COI	BA32	1	BB73946_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73946	Sample	BB73946	targeted	Absent	Sample	Cmae_Bot2011_COI	BA32	COI	45	BB73946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA32	ATGAACAGTCTATCCTCCTTTAG	153.16689
BB73946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37653	WGS84	BB73946	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73946_Msal_Bot2012_COI	BA32	1	BB73946_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73946	Sample	BB73946	targeted	Absent	Sample	Msal_Bot2012_COI	BA32	COI	45	BB73946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA32	GYTAGTTCCRATGATGTTAGCTG	153.16689
BB73948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38147	WGS84	BB73948	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73948_Aamu_Bax2006_COI	BA36	1	BB73948_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73948	Sample	BB73948	targeted	Absent	Sample	Aamu_Bax2006_COI	BA36	COI	45	BB73948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA36	GCACAACCGGGATCTTTACTTC	153.16215
BB73948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38147	WGS84	BB73948	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73948_Pcan_Bot2011_IGS	BA36	1	BB73948_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73948	Sample	BB73948	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA36	IGS	45	BB73948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA36	CGTAATCCTCAGTACTGGCTA	153.16215
BB73948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38147	WGS84	BB73948	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73948_Sspa_Oph2007_COI	BA36	1	BB73948_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73948	Sample	BB73948	targeted	Absent	Sample	Sspa_Oph2007_COI	BA36	COI	45	BB73948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA36	GAAGGCCTTCCGATGATC	153.16215
BB73948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38147	WGS84	BB73948	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73948_Upin_Bot2011_COI	BA36	1	BB73948_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73948	Sample	BB73948	targeted	Absent	Sample	Upin_Bot2011_COI	BA36	COI	45	BB73948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA36	CTTTAATTACAGCGTTTTTATTGTTGT	153.16215
BB73949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8579	WGS84	BB73949	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73949_Mgig_Bot2012_COI	MA43	1	BB73949_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73949	Sample	BB73949	targeted	Absent	Sample	Mgig_Bot2012_COI	MA43	COI	45	BB73949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA43	TCTTATTCGTTGGAGACTTTATAACCCT	144.91104
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.561	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73951_Aamu_Bax2006_COI	MA19	1	BB73951_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Present	Sample	Aamu_Bax2006_COI	MA19	COI	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	GCACAACCGGGATCTTTACTTC	144.90637
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73951_Pcan_Bot2011_IGS	MA19	1	BB73951_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA19	IGS	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	CGTAATCCTCAGTACTGGCTA	144.90637
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.231	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73951_Sspa_Oph2007_COI	MA19	1	BB73951_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Present	Sample	Sspa_Oph2007_COI	MA19	COI	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	GAAGGCCTTCCGATGATC	144.90637
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.733	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73951_Upin_Bot2011_COI	MA19	1	BB73951_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Present	Sample	Upin_Bot2011_COI	MA19	COI	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	CTTTAATTACAGCGTTTTTATTGTTGT	144.90637
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73952_Mgig_Bot2012_COI	MA18	1	BB73952_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Absent	Sample	Mgig_Bot2012_COI	MA18	COI	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	TCTTATTCGTTGGAGACTTTATAACCCT	144.90781
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73953_Cmae_Bot2011_COI	MA16	1	BB73953_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Absent	Sample	Cmae_Bot2011_COI	MA16	COI	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	ATGAACAGTCTATCCTCCTTTAG	144.91541
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73953_Hsan_Wil2021_COI	MA16	1	BB73953_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Absent	Sample	Hsan_Wil2021_COI	MA16	COI	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	CCTGGGCCGGTATAGTAGGT	144.91541
BB73953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86424	WGS84	BB73953	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73953_Msal_Bot2012_COI	MA16	1	BB73953_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73953	Sample	BB73953	targeted	Absent	Sample	Msal_Bot2012_COI	MA16	COI	45	BB73953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA16	GYTAGTTCCRATGATGTTAGCTG	144.91541
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.099	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73955_Aamu_Bax2006_COI	MA13	1	BB73955_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Present	Sample	Aamu_Bax2006_COI	MA13	COI	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	GCACAACCGGGATCTTTACTTC	144.9161
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73955_Pcan_Bot2011_IGS	MA13	1	BB73955_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA13	IGS	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	CGTAATCCTCAGTACTGGCTA	144.9161
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73955_Sspa_Oph2007_COI	MA13	1	BB73955_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Absent	Sample	Sspa_Oph2007_COI	MA13	COI	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	GAAGGCCTTCCGATGATC	144.9161
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73955_Upin_Bot2011_COI	MA13	1	BB73955_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Absent	Sample	Upin_Bot2011_COI	MA13	COI	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	CTTTAATTACAGCGTTTTTATTGTTGT	144.9161
BB73956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38681	WGS84	BB73956	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73956_Mgig_Bot2012_COI	BA40	1	BB73956_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73956	Sample	BB73956	targeted	Absent	Sample	Mgig_Bot2012_COI	BA40	COI	45	BB73956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA40	TCTTATTCGTTGGAGACTTTATAACCCT	153.15913
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.164	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73957_Cmae_Bot2011_COI	MA11	1	BB73957_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Present	Sample	Cmae_Bot2011_COI	MA11	COI	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	ATGAACAGTCTATCCTCCTTTAG	144.91348
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73957_Hsan_Wil2021_COI	MA11	1	BB73957_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Absent	Sample	Hsan_Wil2021_COI	MA11	COI	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	CCTGGGCCGGTATAGTAGGT	144.91348
BB73957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86352	WGS84	BB73957	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73957_Msal_Bot2012_COI	MA11	1	BB73957_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73957	Sample	BB73957	targeted	Absent	Sample	Msal_Bot2012_COI	MA11	COI	45	BB73957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA11	GYTAGTTCCRATGATGTTAGCTG	144.91348
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.55	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73959_Aamu_Bax2006_COI	MA8	1	BB73959_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Present	Sample	Aamu_Bax2006_COI	MA8	COI	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	GCACAACCGGGATCTTTACTTC	144.9099
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73959_Pcan_Bot2011_IGS	MA8	1	BB73959_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA8	IGS	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	CGTAATCCTCAGTACTGGCTA	144.9099
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.611	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73959_Sspa_Oph2007_COI	MA8	1	BB73959_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Present	Sample	Sspa_Oph2007_COI	MA8	COI	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	GAAGGCCTTCCGATGATC	144.9099
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.595	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73959_Upin_Bot2011_COI	MA8	1	BB73959_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Present	Sample	Upin_Bot2011_COI	MA8	COI	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	CTTTAATTACAGCGTTTTTATTGTTGT	144.9099
BB73960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.39185	WGS84	BB73960	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73960_Cmae_Bot2011_COI	BA43	1	BB73960_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73960	Sample	BB73960	targeted	Absent	Sample	Cmae_Bot2011_COI	BA43	COI	45	BB73960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA43	ATGAACAGTCTATCCTCCTTTAG	153.15692
BB73960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.39185	WGS84	BB73960	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73960_Msal_Bot2012_COI	BA43	1	BB73960_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73960	Sample	BB73960	targeted	Absent	Sample	Msal_Bot2012_COI	BA43	COI	45	BB73960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA43	GYTAGTTCCRATGATGTTAGCTG	153.15692
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.557	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73962_Aamu_Bax2006_COI	MA33	1	BB73962_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Present	Sample	Aamu_Bax2006_COI	MA33	COI	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	GCACAACCGGGATCTTTACTTC	144.91212
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73962_Pcan_Bot2011_IGS	MA33	1	BB73962_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA33	IGS	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	CGTAATCCTCAGTACTGGCTA	144.91212
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.122	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73962_Sspa_Oph2007_COI	MA33	1	BB73962_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Present	Sample	Sspa_Oph2007_COI	MA33	COI	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	GAAGGCCTTCCGATGATC	144.91212
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.319	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73962_Upin_Bot2011_COI	MA33	1	BB73962_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Present	Sample	Upin_Bot2011_COI	MA33	COI	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	CTTTAATTACAGCGTTTTTATTGTTGT	144.91212
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73963_Mgig_Bot2012_COI	MA32	1	BB73963_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Absent	Sample	Mgig_Bot2012_COI	MA32	COI	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	TCTTATTCGTTGGAGACTTTATAACCCT	144.90916
BB73964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40078	WGS84	BB73964	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73964_Cmae_Bot2011_COI	BA44	1	BB73964_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73964	Sample	BB73964	targeted	Absent	Sample	Cmae_Bot2011_COI	BA44	COI	45	BB73964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA44	ATGAACAGTCTATCCTCCTTTAG	153.16349
BB73964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40078	WGS84	BB73964	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73964_Msal_Bot2012_COI	BA44	1	BB73964_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73964	Sample	BB73964	targeted	Absent	Sample	Msal_Bot2012_COI	BA44	COI	45	BB73964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA44	GYTAGTTCCRATGATGTTAGCTG	153.16349
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.931	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73966_Aamu_Bax2006_COI	MA27	1	BB73966_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Present	Sample	Aamu_Bax2006_COI	MA27	COI	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	GCACAACCGGGATCTTTACTTC	144.90642
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73966_Pcan_Bot2011_IGS	MA27	1	BB73966_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA27	IGS	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	CGTAATCCTCAGTACTGGCTA	144.90642
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	42.925	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73966_Sspa_Oph2007_COI	MA27	1	BB73966_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Present	Sample	Sspa_Oph2007_COI	MA27	COI	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	GAAGGCCTTCCGATGATC	144.90642
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.051	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73966_Upin_Bot2011_COI	MA27	1	BB73966_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Present	Sample	Upin_Bot2011_COI	MA27	COI	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	CTTTAATTACAGCGTTTTTATTGTTGT	144.90642
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.754	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73967_Mgig_Bot2012_COI	MA26	1	BB73967_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Present	Sample	Mgig_Bot2012_COI	MA26	COI	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	TCTTATTCGTTGGAGACTTTATAACCCT	144.90739
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73968_Cmae_Bot2011_COI	MA24	1	BB73968_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Absent	Sample	Cmae_Bot2011_COI	MA24	COI	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	ATGAACAGTCTATCCTCCTTTAG	144.90697
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73968_Hsan_Wil2021_COI	MA24	1	BB73968_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Absent	Sample	Hsan_Wil2021_COI	MA24	COI	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	CCTGGGCCGGTATAGTAGGT	144.90697
BB73968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84629	WGS84	BB73968	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73968_Msal_Bot2012_COI	MA24	1	BB73968_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73968	Sample	BB73968	targeted	Absent	Sample	Msal_Bot2012_COI	MA24	COI	45	BB73968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA24	GYTAGTTCCRATGATGTTAGCTG	144.90697
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73970_Mgig_Bot2012_COI	MA22	1	BB73970_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Absent	Sample	Mgig_Bot2012_COI	MA22	COI	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	TCTTATTCGTTGGAGACTTTATAACCCT	144.90906
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73971_Cmae_Bot2011_COI	MA21	1	BB73971_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Absent	Sample	Cmae_Bot2011_COI	MA21	COI	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	ATGAACAGTCTATCCTCCTTTAG	144.91224
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73971_Hsan_Wil2021_COI	MA21	1	BB73971_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Absent	Sample	Hsan_Wil2021_COI	MA21	COI	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	CCTGGGCCGGTATAGTAGGT	144.91224
BB73971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85143	WGS84	BB73971	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73971_Msal_Bot2012_COI	MA21	1	BB73971_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73971	Sample	BB73971	targeted	Absent	Sample	Msal_Bot2012_COI	MA21	COI	45	BB73971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA21	GYTAGTTCCRATGATGTTAGCTG	144.91224
BB73973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40277	WGS84	BB73973	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73973_Aamu_Bax2006_COI	BA49	1	BB73973_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73973	Sample	BB73973	targeted	Absent	Sample	Aamu_Bax2006_COI	BA49	COI	45	BB73973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA49	GCACAACCGGGATCTTTACTTC	153.15406
BB73973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40277	WGS84	BB73973	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73973_Pcan_Bot2011_IGS	BA49	1	BB73973_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73973	Sample	BB73973	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA49	IGS	45	BB73973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA49	CGTAATCCTCAGTACTGGCTA	153.15406
BB73973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40277	WGS84	BB73973	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73973_Sspa_Oph2007_COI	BA49	1	BB73973_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73973	Sample	BB73973	targeted	Absent	Sample	Sspa_Oph2007_COI	BA49	COI	45	BB73973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA49	GAAGGCCTTCCGATGATC	153.15406
BB73973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40277	WGS84	BB73973	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73973_Upin_Bot2011_COI	BA49	1	BB73973_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73973	Sample	BB73973	targeted	Absent	Sample	Upin_Bot2011_COI	BA49	COI	45	BB73973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA49	CTTTAATTACAGCGTTTTTATTGTTGT	153.15406
BB73974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40781	WGS84	BB73974	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73974_Mgig_Bot2012_COI	BA52	1	BB73974_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73974	Sample	BB73974	targeted	Absent	Sample	Mgig_Bot2012_COI	BA52	COI	45	BB73974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA52	TCTTATTCGTTGGAGACTTTATAACCCT	153.1502
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73975_Cmae_Bot2011_COI	MA121	1	BB73975_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Absent	Sample	Cmae_Bot2011_COI	MA121	COI	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	ATGAACAGTCTATCCTCCTTTAG	144.93272
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73975_Hsan_Wil2021_COI	MA121	1	BB73975_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Absent	Sample	Hsan_Wil2021_COI	MA121	COI	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	CCTGGGCCGGTATAGTAGGT	144.93272
BB73975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84406	WGS84	BB73975	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73975_Msal_Bot2012_COI	MA121	1	BB73975_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73975	Sample	BB73975	targeted	Absent	Sample	Msal_Bot2012_COI	MA121	COI	45	BB73975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA121	GYTAGTTCCRATGATGTTAGCTG	144.93272
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.432	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73977_Aamu_Bax2006_COI	MA116	1	BB73977_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Present	Sample	Aamu_Bax2006_COI	MA116	COI	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	GCACAACCGGGATCTTTACTTC	144.93082
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73977_Pcan_Bot2011_IGS	MA116	1	BB73977_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA116	IGS	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	CGTAATCCTCAGTACTGGCTA	144.93082
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.665	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73977_Sspa_Oph2007_COI	MA116	1	BB73977_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Present	Sample	Sspa_Oph2007_COI	MA116	COI	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	GAAGGCCTTCCGATGATC	144.93082
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.56	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73977_Upin_Bot2011_COI	MA116	1	BB73977_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Present	Sample	Upin_Bot2011_COI	MA116	COI	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	CTTTAATTACAGCGTTTTTATTGTTGT	144.93082
BB73978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.41523	WGS84	BB73978	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73978_Mgig_Bot2012_COI	BA53	1	BB73978_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73978	Sample	BB73978	targeted	Absent	Sample	Mgig_Bot2012_COI	BA53	COI	45	BB73978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA53	TCTTATTCGTTGGAGACTTTATAACCCT	153.14303
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73979_Cmae_Bot2011_COI	MA113	1	BB73979_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Absent	Sample	Cmae_Bot2011_COI	MA113	COI	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	ATGAACAGTCTATCCTCCTTTAG	144.92943
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73979_Hsan_Wil2021_COI	MA113	1	BB73979_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Absent	Sample	Hsan_Wil2021_COI	MA113	COI	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	CCTGGGCCGGTATAGTAGGT	144.92943
BB73979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84253	WGS84	BB73979	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73979_Msal_Bot2012_COI	MA113	1	BB73979_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73979	Sample	BB73979	targeted	Absent	Sample	Msal_Bot2012_COI	MA113	COI	45	BB73979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA113	GYTAGTTCCRATGATGTTAGCTG	144.92943
BB73980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42093	WGS84	BB73980	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73980_Aamu_Bax2006_COI	BA54	1	BB73980_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73980	Sample	BB73980	targeted	Absent	Sample	Aamu_Bax2006_COI	BA54	COI	45	BB73980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA54	GCACAACCGGGATCTTTACTTC	153.13708
BB73980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42093	WGS84	BB73980	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73980_Pcan_Bot2011_IGS	BA54	1	BB73980_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73980	Sample	BB73980	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA54	IGS	45	BB73980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA54	CGTAATCCTCAGTACTGGCTA	153.13708
BB73980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42093	WGS84	BB73980	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73980_Sspa_Oph2007_COI	BA54	1	BB73980_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73980	Sample	BB73980	targeted	Absent	Sample	Sspa_Oph2007_COI	BA54	COI	45	BB73980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA54	GAAGGCCTTCCGATGATC	153.13708
BB73980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42093	WGS84	BB73980	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73980_Upin_Bot2011_COI	BA54	1	BB73980_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73980	Sample	BB73980	targeted	Absent	Sample	Upin_Bot2011_COI	BA54	COI	45	BB73980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA54	CTTTAATTACAGCGTTTTTATTGTTGT	153.13708
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73981_Mgig_Bot2012_COI	MA108	1	BB73981_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Absent	Sample	Mgig_Bot2012_COI	MA108	COI	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	TCTTATTCGTTGGAGACTTTATAACCCT	144.92753
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73982_Cmae_Bot2011_COI	MA66	1	BB73982_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Absent	Sample	Cmae_Bot2011_COI	MA66	COI	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	ATGAACAGTCTATCCTCCTTTAG	144.90195
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73982_Hsan_Wil2021_COI	MA66	1	BB73982_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Absent	Sample	Hsan_Wil2021_COI	MA66	COI	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	CCTGGGCCGGTATAGTAGGT	144.90195
BB73982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82698	WGS84	BB73982	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73982_Msal_Bot2012_COI	MA66	1	BB73982_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73982	Sample	BB73982	targeted	Absent	Sample	Msal_Bot2012_COI	MA66	COI	45	BB73982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA66	GYTAGTTCCRATGATGTTAGCTG	144.90195
BB73984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80612	WGS84	BB73984	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73984_Aamu_Bax2006_COI	GA11	1	BB73984_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73984	Sample	BB73984	targeted	Absent	Sample	Aamu_Bax2006_COI	GA11	COI	45	BB73984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA11	GCACAACCGGGATCTTTACTTC	151.21916
BB73984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80612	WGS84	BB73984	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73984_Pcan_Bot2011_IGS	GA11	1	BB73984_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73984	Sample	BB73984	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA11	IGS	45	BB73984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA11	CGTAATCCTCAGTACTGGCTA	151.21916
BB73984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80612	WGS84	BB73984	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73984_Sspa_Oph2007_COI	GA11	1	BB73984_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73984	Sample	BB73984	targeted	Absent	Sample	Sspa_Oph2007_COI	GA11	COI	45	BB73984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA11	GAAGGCCTTCCGATGATC	151.21916
BB73984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80612	WGS84	BB73984	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73984_Upin_Bot2011_COI	GA11	1	BB73984_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73984	Sample	BB73984	targeted	Absent	Sample	Upin_Bot2011_COI	GA11	COI	45	BB73984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA11	CTTTAATTACAGCGTTTTTATTGTTGT	151.21916
BB73985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80663	WGS84	BB73985	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73985_Mgig_Bot2012_COI	GA9	1	BB73985_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73985	Sample	BB73985	targeted	Absent	Sample	Mgig_Bot2012_COI	GA9	COI	45	BB73985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA9	TCTTATTCGTTGGAGACTTTATAACCCT	151.21743
BB73986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42312	WGS84	BB73986	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73986_Cmae_Bot2011_COI	BA56	1	BB73986_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73986	Sample	BB73986	targeted	Absent	Sample	Cmae_Bot2011_COI	BA56	COI	45	BB73986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA56	ATGAACAGTCTATCCTCCTTTAG	153.13199
BB73986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42312	WGS84	BB73986	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73986_Msal_Bot2012_COI	BA56	1	BB73986_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73986	Sample	BB73986	targeted	Absent	Sample	Msal_Bot2012_COI	BA56	COI	45	BB73986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA56	GYTAGTTCCRATGATGTTAGCTG	153.13199
BB73988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42525	WGS84	BB73988	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73988_Aamu_Bax2006_COI	BA57	1	BB73988_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73988	Sample	BB73988	targeted	Absent	Sample	Aamu_Bax2006_COI	BA57	COI	45	BB73988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA57	GCACAACCGGGATCTTTACTTC	153.12768
BB73988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42525	WGS84	BB73988	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73988_Pcan_Bot2011_IGS	BA57	1	BB73988_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73988	Sample	BB73988	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA57	IGS	45	BB73988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA57	CGTAATCCTCAGTACTGGCTA	153.12768
BB73988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42525	WGS84	BB73988	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73988_Sspa_Oph2007_COI	BA57	1	BB73988_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73988	Sample	BB73988	targeted	Absent	Sample	Sspa_Oph2007_COI	BA57	COI	45	BB73988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA57	GAAGGCCTTCCGATGATC	153.12768
BB73988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42525	WGS84	BB73988	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73988_Upin_Bot2011_COI	BA57	1	BB73988_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73988	Sample	BB73988	targeted	Absent	Sample	Upin_Bot2011_COI	BA57	COI	45	BB73988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA57	CTTTAATTACAGCGTTTTTATTGTTGT	153.12768
BB73989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80729	WGS84	BB73989	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73989_Mgig_Bot2012_COI	GA4	1	BB73989_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73989	Sample	BB73989	targeted	Absent	Sample	Mgig_Bot2012_COI	GA4	COI	45	BB73989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA4	TCTTATTCGTTGGAGACTTTATAACCCT	151.21688
BB73991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80628	WGS84	BB73991	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73991_Aamu_Bax2006_COI	GA2	1	BB73991_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73991	Sample	BB73991	targeted	Absent	Sample	Aamu_Bax2006_COI	GA2	COI	45	BB73991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA2	GCACAACCGGGATCTTTACTTC	151.21494
BB73991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80628	WGS84	BB73991	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73991_Pcan_Bot2011_IGS	GA2	1	BB73991_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73991	Sample	BB73991	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA2	IGS	45	BB73991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA2	CGTAATCCTCAGTACTGGCTA	151.21494
BB73991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80628	WGS84	BB73991	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73991_Sspa_Oph2007_COI	GA2	1	BB73991_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73991	Sample	BB73991	targeted	Absent	Sample	Sspa_Oph2007_COI	GA2	COI	45	BB73991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA2	GAAGGCCTTCCGATGATC	151.21494
BB73991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80628	WGS84	BB73991	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73991_Upin_Bot2011_COI	GA2	1	BB73991_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73991	Sample	BB73991	targeted	Absent	Sample	Upin_Bot2011_COI	GA2	COI	45	BB73991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA2	CTTTAATTACAGCGTTTTTATTGTTGT	151.21494
BB73992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42892	WGS84	BB73992	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73992_Mgig_Bot2012_COI	BA64	1	BB73992_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73992	Sample	BB73992	targeted	Absent	Sample	Mgig_Bot2012_COI	BA64	COI	45	BB73992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA64	TCTTATTCGTTGGAGACTTTATAACCCT	153.12154
BB73993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85375	WGS84	BB73993	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73993_Cmae_Bot2011_COI	MA35	1	BB73993_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73993	Sample	BB73993	targeted	Absent	Sample	Cmae_Bot2011_COI	MA35	COI	45	BB73993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA35	ATGAACAGTCTATCCTCCTTTAG	151.30827
BB73993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85375	WGS84	BB73993	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73993_Msal_Bot2012_COI	MA35	1	BB73993_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73993	Sample	BB73993	targeted	Absent	Sample	Msal_Bot2012_COI	MA35	COI	45	BB73993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA35	GYTAGTTCCRATGATGTTAGCTG	151.30827
BB73995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85533	WGS84	BB73995	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73995_Aamu_Bax2006_COI	MA25	1	BB73995_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73995	Sample	BB73995	targeted	Absent	Sample	Aamu_Bax2006_COI	MA25	COI	45	BB73995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA25	GCACAACCGGGATCTTTACTTC	151.32178
BB73995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85533	WGS84	BB73995	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73995_Pcan_Bot2011_IGS	MA25	1	BB73995_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73995	Sample	BB73995	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA25	IGS	45	BB73995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA25	CGTAATCCTCAGTACTGGCTA	151.32178
BB73995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85533	WGS84	BB73995	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73995_Sspa_Oph2007_COI	MA25	1	BB73995_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73995	Sample	BB73995	targeted	Absent	Sample	Sspa_Oph2007_COI	MA25	COI	45	BB73995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA25	GAAGGCCTTCCGATGATC	151.32178
BB73995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85533	WGS84	BB73995	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73995_Upin_Bot2011_COI	MA25	1	BB73995_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73995	Sample	BB73995	targeted	Absent	Sample	Upin_Bot2011_COI	MA25	COI	45	BB73995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA25	CTTTAATTACAGCGTTTTTATTGTTGT	151.32178
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.749	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73996_Mgig_Bot2012_COI	MA97	1	BB73996_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Present	Sample	Mgig_Bot2012_COI	MA97	COI	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	TCTTATTCGTTGGAGACTTTATAACCCT	144.93099
BB73997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85066	WGS84	BB73997	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73997_Cmae_Bot2011_COI	GA33	1	BB73997_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73997	Sample	BB73997	targeted	Absent	Sample	Cmae_Bot2011_COI	GA33	COI	45	BB73997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA33	ATGAACAGTCTATCCTCCTTTAG	151.31174
BB73997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85066	WGS84	BB73997	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73997_Msal_Bot2012_COI	GA33	1	BB73997_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73997	Sample	BB73997	targeted	Absent	Sample	Msal_Bot2012_COI	GA33	COI	45	BB73997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA33	GYTAGTTCCRATGATGTTAGCTG	151.31174
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.898	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73999_Aamu_Bax2006_COI	MA39	1	BB73999_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Present	Sample	Aamu_Bax2006_COI	MA39	COI	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	GCACAACCGGGATCTTTACTTC	144.90391
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73999_Pcan_Bot2011_IGS	MA39	1	BB73999_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA39	IGS	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	CGTAATCCTCAGTACTGGCTA	144.90391
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.403	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73999_Sspa_Oph2007_COI	MA39	1	BB73999_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Present	Sample	Sspa_Oph2007_COI	MA39	COI	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	GAAGGCCTTCCGATGATC	144.90391
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.976	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73999_Upin_Bot2011_COI	MA39	1	BB73999_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Present	Sample	Upin_Bot2011_COI	MA39	COI	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	CTTTAATTACAGCGTTTTTATTGTTGT	144.90391
BB74000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85211	WGS84	BB74000	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74000_Cmae_Bot2011_COI	GA29	1	BB74000_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74000	Sample	BB74000	targeted	Absent	Sample	Cmae_Bot2011_COI	GA29	COI	45	BB74000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA29	ATGAACAGTCTATCCTCCTTTAG	151.31866
BB74000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85211	WGS84	BB74000	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74000_Msal_Bot2012_COI	GA29	1	BB74000_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74000	Sample	BB74000	targeted	Absent	Sample	Msal_Bot2012_COI	GA29	COI	45	BB74000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA29	GYTAGTTCCRATGATGTTAGCTG	151.31866
BB74002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85391	WGS84	BB74002	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74002_Aamu_Bax2006_COI	GA27	1	BB74002_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74002	Sample	BB74002	targeted	Absent	Sample	Aamu_Bax2006_COI	GA27	COI	45	BB74002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA27	GCACAACCGGGATCTTTACTTC	151.31835
BB74002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85391	WGS84	BB74002	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74002_Pcan_Bot2011_IGS	GA27	1	BB74002_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74002	Sample	BB74002	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA27	IGS	45	BB74002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA27	CGTAATCCTCAGTACTGGCTA	151.31835
BB74002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85391	WGS84	BB74002	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74002_Sspa_Oph2007_COI	GA27	1	BB74002_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74002	Sample	BB74002	targeted	Absent	Sample	Sspa_Oph2007_COI	GA27	COI	45	BB74002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA27	GAAGGCCTTCCGATGATC	151.31835
BB74002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85391	WGS84	BB74002	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74002_Upin_Bot2011_COI	GA27	1	BB74002_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74002	Sample	BB74002	targeted	Absent	Sample	Upin_Bot2011_COI	GA27	COI	45	BB74002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA27	CTTTAATTACAGCGTTTTTATTGTTGT	151.31835
BB74003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8401	WGS84	BB74003	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74003_Mgig_Bot2012_COI	MA41	1	BB74003_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74003	Sample	BB74003	targeted	Absent	Sample	Mgig_Bot2012_COI	MA41	COI	45	BB74003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA41	TCTTATTCGTTGGAGACTTTATAACCCT	144.90565
BB74004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85331	WGS84	BB74004	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74004_Cmae_Bot2011_COI	GA24	1	BB74004_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74004	Sample	BB74004	targeted	Absent	Sample	Cmae_Bot2011_COI	GA24	COI	45	BB74004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA24	ATGAACAGTCTATCCTCCTTTAG	151.32146
BB74004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85331	WGS84	BB74004	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74004_Msal_Bot2012_COI	GA24	1	BB74004_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74004	Sample	BB74004	targeted	Absent	Sample	Msal_Bot2012_COI	GA24	COI	45	BB74004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA24	GYTAGTTCCRATGATGTTAGCTG	151.32146
BB74006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8879	WGS84	BB74006	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.704	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74006_Aamu_Bax2006_COI	HA4	1	BB74006_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74006	Sample	BB74006	targeted	Present	Sample	Aamu_Bax2006_COI	HA4	COI	45	BB74006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA4	GCACAACCGGGATCTTTACTTC	147.3418
BB74006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8879	WGS84	BB74006	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74006_Pcan_Bot2011_IGS	HA4	1	BB74006_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74006	Sample	BB74006	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA4	IGS	45	BB74006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA4	CGTAATCCTCAGTACTGGCTA	147.3418
BB74006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8879	WGS84	BB74006	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74006_Sspa_Oph2007_COI	HA4	1	BB74006_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74006	Sample	BB74006	targeted	Absent	Sample	Sspa_Oph2007_COI	HA4	COI	45	BB74006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA4	GAAGGCCTTCCGATGATC	147.3418
BB74006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8879	WGS84	BB74006	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74006_Upin_Bot2011_COI	HA4	1	BB74006_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74006	Sample	BB74006	targeted	Absent	Sample	Upin_Bot2011_COI	HA4	COI	45	BB74006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA4	CTTTAATTACAGCGTTTTTATTGTTGT	147.3418
BB74007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78588	WGS84	BB74007	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74007_Mgig_Bot2012_COI	MA60	1	BB74007_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74007	Sample	BB74007	targeted	Absent	Sample	Mgig_Bot2012_COI	MA60	COI	45	BB74007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA60	TCTTATTCGTTGGAGACTTTATAACCCT	151.17218
BB74008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78775	WGS84	BB74008	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74008_Cmae_Bot2011_COI	MA55	1	BB74008_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74008	Sample	BB74008	targeted	Absent	Sample	Cmae_Bot2011_COI	MA55	COI	45	BB74008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA55	ATGAACAGTCTATCCTCCTTTAG	151.17457
BB74008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78775	WGS84	BB74008	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74008_Msal_Bot2012_COI	MA55	1	BB74008_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74008	Sample	BB74008	targeted	Absent	Sample	Msal_Bot2012_COI	MA55	COI	45	BB74008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA55	GYTAGTTCCRATGATGTTAGCTG	151.17457
BB74010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.794	WGS84	BB74010	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74010_Mgig_Bot2012_COI	MA45	1	BB74010_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74010	Sample	BB74010	targeted	Absent	Sample	Mgig_Bot2012_COI	MA45	COI	45	BB74010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA45	TCTTATTCGTTGGAGACTTTATAACCCT	151.1844
BB74011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78735	WGS84	BB74011	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74011_Cmae_Bot2011_COI	GA62	1	BB74011_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74011	Sample	BB74011	targeted	Absent	Sample	Cmae_Bot2011_COI	GA62	COI	45	BB74011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA62	ATGAACAGTCTATCCTCCTTTAG	151.17788
BB74011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78735	WGS84	BB74011	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74011_Msal_Bot2012_COI	GA62	1	BB74011_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74011	Sample	BB74011	targeted	Absent	Sample	Msal_Bot2012_COI	GA62	COI	45	BB74011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA62	GYTAGTTCCRATGATGTTAGCTG	151.17788
BB74013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87954	WGS84	BB74013	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.714	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74013_Aamu_Bax2006_COI	HA7	1	BB74013_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74013	Sample	BB74013	targeted	Present	Sample	Aamu_Bax2006_COI	HA7	COI	45	BB74013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA7	GCACAACCGGGATCTTTACTTC	147.34488
BB74013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87954	WGS84	BB74013	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74013_Pcan_Bot2011_IGS	HA7	1	BB74013_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74013	Sample	BB74013	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA7	IGS	45	BB74013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA7	CGTAATCCTCAGTACTGGCTA	147.34488
BB74013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87954	WGS84	BB74013	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74013_Sspa_Oph2007_COI	HA7	1	BB74013_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74013	Sample	BB74013	targeted	Absent	Sample	Sspa_Oph2007_COI	HA7	COI	45	BB74013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA7	GAAGGCCTTCCGATGATC	147.34488
BB74013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87954	WGS84	BB74013	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74013_Upin_Bot2011_COI	HA7	1	BB74013_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74013	Sample	BB74013	targeted	Absent	Sample	Upin_Bot2011_COI	HA7	COI	45	BB74013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA7	CTTTAATTACAGCGTTTTTATTGTTGT	147.34488
BB74014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78625	WGS84	BB74014	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74014_Mgig_Bot2012_COI	GA57	1	BB74014_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74014	Sample	BB74014	targeted	Absent	Sample	Mgig_Bot2012_COI	GA57	COI	45	BB74014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA57	TCTTATTCGTTGGAGACTTTATAACCCT	151.17631
BB74015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78668	WGS84	BB74015	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74015_Cmae_Bot2011_COI	GA56	1	BB74015_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74015	Sample	BB74015	targeted	Absent	Sample	Cmae_Bot2011_COI	GA56	COI	45	BB74015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA56	ATGAACAGTCTATCCTCCTTTAG	151.17519
BB74015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78668	WGS84	BB74015	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74015_Msal_Bot2012_COI	GA56	1	BB74015_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74015	Sample	BB74015	targeted	Absent	Sample	Msal_Bot2012_COI	GA56	COI	45	BB74015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA56	GYTAGTTCCRATGATGTTAGCTG	151.17519
BB74017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78949	WGS84	BB74017	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74017_Aamu_Bax2006_COI	GA53	1	BB74017_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74017	Sample	BB74017	targeted	Absent	Sample	Aamu_Bax2006_COI	GA53	COI	45	BB74017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA53	GCACAACCGGGATCTTTACTTC	151.17594
BB74017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78949	WGS84	BB74017	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74017_Pcan_Bot2011_IGS	GA53	1	BB74017_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74017	Sample	BB74017	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA53	IGS	45	BB74017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA53	CGTAATCCTCAGTACTGGCTA	151.17594
BB74017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78949	WGS84	BB74017	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74017_Sspa_Oph2007_COI	GA53	1	BB74017_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74017	Sample	BB74017	targeted	Absent	Sample	Sspa_Oph2007_COI	GA53	COI	45	BB74017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA53	GAAGGCCTTCCGATGATC	151.17594
BB74017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78949	WGS84	BB74017	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74017_Upin_Bot2011_COI	GA53	1	BB74017_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74017	Sample	BB74017	targeted	Absent	Sample	Upin_Bot2011_COI	GA53	COI	45	BB74017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA53	CTTTAATTACAGCGTTTTTATTGTTGT	151.17594
BB74018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88512	WGS84	BB74018	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74018_Mgig_Bot2012_COI	HA9	1	BB74018_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74018	Sample	BB74018	targeted	Absent	Sample	Mgig_Bot2012_COI	HA9	COI	45	BB74018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA9	TCTTATTCGTTGGAGACTTTATAACCCT	147.33365
BB74019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78956	WGS84	BB74019	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74019_Cmae_Bot2011_COI	GA51	1	BB74019_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74019	Sample	BB74019	targeted	Absent	Sample	Cmae_Bot2011_COI	GA51	COI	45	BB74019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA51	ATGAACAGTCTATCCTCCTTTAG	151.17817
BB74019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78956	WGS84	BB74019	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74019_Msal_Bot2012_COI	GA51	1	BB74019_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74019	Sample	BB74019	targeted	Absent	Sample	Msal_Bot2012_COI	GA51	COI	45	BB74019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA51	GYTAGTTCCRATGATGTTAGCTG	151.17817
BB74020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79134	WGS84	BB74020	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74020_Aamu_Bax2006_COI	GA49	1	BB74020_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74020	Sample	BB74020	targeted	Absent	Sample	Aamu_Bax2006_COI	GA49	COI	45	BB74020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA49	GCACAACCGGGATCTTTACTTC	151.18417
BB74020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79134	WGS84	BB74020	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74020_Pcan_Bot2011_IGS	GA49	1	BB74020_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74020	Sample	BB74020	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA49	IGS	45	BB74020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA49	CGTAATCCTCAGTACTGGCTA	151.18417
BB74020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79134	WGS84	BB74020	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74020_Sspa_Oph2007_COI	GA49	1	BB74020_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74020	Sample	BB74020	targeted	Absent	Sample	Sspa_Oph2007_COI	GA49	COI	45	BB74020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA49	GAAGGCCTTCCGATGATC	151.18417
BB74020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79134	WGS84	BB74020	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74020_Upin_Bot2011_COI	GA49	1	BB74020_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74020	Sample	BB74020	targeted	Absent	Sample	Upin_Bot2011_COI	GA49	COI	45	BB74020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA49	CTTTAATTACAGCGTTTTTATTGTTGT	151.18417
BB74021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7912	WGS84	BB74021	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74021_Mgig_Bot2012_COI	GA48	1	BB74021_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74021	Sample	BB74021	targeted	Absent	Sample	Mgig_Bot2012_COI	GA48	COI	45	BB74021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA48	TCTTATTCGTTGGAGACTTTATAACCCT	151.17933
BB74022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79159	WGS84	BB74022	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74022_Cmae_Bot2011_COI	GA47	1	BB74022_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74022	Sample	BB74022	targeted	Absent	Sample	Cmae_Bot2011_COI	GA47	COI	45	BB74022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA47	ATGAACAGTCTATCCTCCTTTAG	151.1805
BB74022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79159	WGS84	BB74022	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74022_Msal_Bot2012_COI	GA47	1	BB74022_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74022	Sample	BB74022	targeted	Absent	Sample	Msal_Bot2012_COI	GA47	COI	45	BB74022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA47	GYTAGTTCCRATGATGTTAGCTG	151.1805
BB74024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78496	WGS84	BB74024	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74024_Aamu_Bax2006_COI	MA70	1	BB74024_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74024	Sample	BB74024	targeted	Absent	Sample	Aamu_Bax2006_COI	MA70	COI	45	BB74024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA70	GCACAACCGGGATCTTTACTTC	151.20333
BB74024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78496	WGS84	BB74024	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74024_Pcan_Bot2011_IGS	MA70	1	BB74024_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74024	Sample	BB74024	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA70	IGS	45	BB74024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA70	CGTAATCCTCAGTACTGGCTA	151.20333
BB74024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78496	WGS84	BB74024	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74024_Sspa_Oph2007_COI	MA70	1	BB74024_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74024	Sample	BB74024	targeted	Absent	Sample	Sspa_Oph2007_COI	MA70	COI	45	BB74024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA70	GAAGGCCTTCCGATGATC	151.20333
BB74024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78496	WGS84	BB74024	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74024_Upin_Bot2011_COI	MA70	1	BB74024_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74024	Sample	BB74024	targeted	Absent	Sample	Upin_Bot2011_COI	MA70	COI	45	BB74024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA70	CTTTAATTACAGCGTTTTTATTGTTGT	151.20333
BB74025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77966	WGS84	BB74025	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74025_Mgig_Bot2012_COI	MA65	1	BB74025_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74025	Sample	BB74025	targeted	Absent	Sample	Mgig_Bot2012_COI	MA65	COI	45	BB74025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA65	TCTTATTCGTTGGAGACTTTATAACCCT	151.19784
BB74026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84997	WGS84	BB74026	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74026_Cmae_Bot2011_COI	HA14	1	BB74026_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74026	Sample	BB74026	targeted	Absent	Sample	Cmae_Bot2011_COI	HA14	COI	45	BB74026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA14	ATGAACAGTCTATCCTCCTTTAG	147.33006
BB74026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84997	WGS84	BB74026	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74026_Msal_Bot2012_COI	HA14	1	BB74026_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74026	Sample	BB74026	targeted	Absent	Sample	Msal_Bot2012_COI	HA14	COI	45	BB74026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA14	GYTAGTTCCRATGATGTTAGCTG	147.33006
BB74028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7813	WGS84	BB74028	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74028_Aamu_Bax2006_COI	GA67	1	BB74028_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74028	Sample	BB74028	targeted	Absent	Sample	Aamu_Bax2006_COI	GA67	COI	45	BB74028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA67	GCACAACCGGGATCTTTACTTC	151.1994
BB74028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7813	WGS84	BB74028	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74028_Pcan_Bot2011_IGS	GA67	1	BB74028_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74028	Sample	BB74028	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA67	IGS	45	BB74028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA67	CGTAATCCTCAGTACTGGCTA	151.1994
BB74028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7813	WGS84	BB74028	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74028_Sspa_Oph2007_COI	GA67	1	BB74028_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74028	Sample	BB74028	targeted	Absent	Sample	Sspa_Oph2007_COI	GA67	COI	45	BB74028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA67	GAAGGCCTTCCGATGATC	151.1994
BB74028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7813	WGS84	BB74028	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74028_Upin_Bot2011_COI	GA67	1	BB74028_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74028	Sample	BB74028	targeted	Absent	Sample	Upin_Bot2011_COI	GA67	COI	45	BB74028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA67	CTTTAATTACAGCGTTTTTATTGTTGT	151.1994
BB74029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84711	WGS84	BB74029	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74029_Mgig_Bot2012_COI	HA16	1	BB74029_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74029	Sample	BB74029	targeted	Absent	Sample	Mgig_Bot2012_COI	HA16	COI	45	BB74029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA16	TCTTATTCGTTGGAGACTTTATAACCCT	147.32992
BB74031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77756	WGS84	BB74031	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74031_Aamu_Bax2006_COI	GA63	1	BB74031_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74031	Sample	BB74031	targeted	Absent	Sample	Aamu_Bax2006_COI	GA63	COI	45	BB74031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA63	GCACAACCGGGATCTTTACTTC	151.19502
BB74031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77756	WGS84	BB74031	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74031_Pcan_Bot2011_IGS	GA63	1	BB74031_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74031	Sample	BB74031	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA63	IGS	45	BB74031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA63	CGTAATCCTCAGTACTGGCTA	151.19502
BB74031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77756	WGS84	BB74031	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74031_Sspa_Oph2007_COI	GA63	1	BB74031_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74031	Sample	BB74031	targeted	Absent	Sample	Sspa_Oph2007_COI	GA63	COI	45	BB74031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA63	GAAGGCCTTCCGATGATC	151.19502
BB74031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77756	WGS84	BB74031	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74031_Upin_Bot2011_COI	GA63	1	BB74031_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74031	Sample	BB74031	targeted	Absent	Sample	Upin_Bot2011_COI	GA63	COI	45	BB74031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA63	CTTTAATTACAGCGTTTTTATTGTTGT	151.19502
BB74032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84416	WGS84	BB74032	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74032_Mgig_Bot2012_COI	HA18	1	BB74032_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74032	Sample	BB74032	targeted	Absent	Sample	Mgig_Bot2012_COI	HA18	COI	45	BB74032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA18	TCTTATTCGTTGGAGACTTTATAACCCT	147.32978
BB74033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79513	WGS84	BB74033	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74033_Cmae_Bot2011_COI	GA111	1	BB74033_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74033	Sample	BB74033	targeted	Absent	Sample	Cmae_Bot2011_COI	GA111	COI	45	BB74033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA111	ATGAACAGTCTATCCTCCTTTAG	151.21416
BB74033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79513	WGS84	BB74033	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74033_Msal_Bot2012_COI	GA111	1	BB74033_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74033	Sample	BB74033	targeted	Absent	Sample	Msal_Bot2012_COI	GA111	COI	45	BB74033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA111	GYTAGTTCCRATGATGTTAGCTG	151.21416
BB74035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79115	WGS84	BB74035	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74035_Aamu_Bax2006_COI	GA72	1	BB74035_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74035	Sample	BB74035	targeted	Absent	Sample	Aamu_Bax2006_COI	GA72	COI	45	BB74035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA72	GCACAACCGGGATCTTTACTTC	151.2105
BB74035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79115	WGS84	BB74035	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74035_Pcan_Bot2011_IGS	GA72	1	BB74035_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74035	Sample	BB74035	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA72	IGS	45	BB74035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA72	CGTAATCCTCAGTACTGGCTA	151.2105
BB74035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79115	WGS84	BB74035	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74035_Sspa_Oph2007_COI	GA72	1	BB74035_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74035	Sample	BB74035	targeted	Absent	Sample	Sspa_Oph2007_COI	GA72	COI	45	BB74035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA72	GAAGGCCTTCCGATGATC	151.2105
BB74035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79115	WGS84	BB74035	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74035_Upin_Bot2011_COI	GA72	1	BB74035_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74035	Sample	BB74035	targeted	Absent	Sample	Upin_Bot2011_COI	GA72	COI	45	BB74035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA72	CTTTAATTACAGCGTTTTTATTGTTGT	151.2105
BB74036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79117	WGS84	BB74036	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74036_Mgig_Bot2012_COI	GA71	1	BB74036_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74036	Sample	BB74036	targeted	Absent	Sample	Mgig_Bot2012_COI	GA71	COI	45	BB74036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA71	TCTTATTCGTTGGAGACTTTATAACCCT	151.21073
BB74037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82416	WGS84	BB74037	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74037_Cmae_Bot2011_COI	GA103	1	BB74037_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74037	Sample	BB74037	targeted	Absent	Sample	Cmae_Bot2011_COI	GA103	COI	45	BB74037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA103	ATGAACAGTCTATCCTCCTTTAG	151.24835
BB74037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82416	WGS84	BB74037	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74037_Msal_Bot2012_COI	GA103	1	BB74037_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74037	Sample	BB74037	targeted	Absent	Sample	Msal_Bot2012_COI	GA103	COI	45	BB74037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA103	GYTAGTTCCRATGATGTTAGCTG	151.24835
BB74039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.83217	WGS84	BB74039	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74039_Aamu_Bax2006_COI	HA24	1	BB74039_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74039	Sample	BB74039	targeted	Absent	Sample	Aamu_Bax2006_COI	HA24	COI	45	BB74039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA24	GCACAACCGGGATCTTTACTTC	147.32211
BB74039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.83217	WGS84	BB74039	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74039_Pcan_Bot2011_IGS	HA24	1	BB74039_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74039	Sample	BB74039	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA24	IGS	45	BB74039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA24	CGTAATCCTCAGTACTGGCTA	147.32211
BB74039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.83217	WGS84	BB74039	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74039_Sspa_Oph2007_COI	HA24	1	BB74039_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74039	Sample	BB74039	targeted	Absent	Sample	Sspa_Oph2007_COI	HA24	COI	45	BB74039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA24	GAAGGCCTTCCGATGATC	147.32211
BB74039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.83217	WGS84	BB74039	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74039_Upin_Bot2011_COI	HA24	1	BB74039_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74039	Sample	BB74039	targeted	Absent	Sample	Upin_Bot2011_COI	HA24	COI	45	BB74039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA24	CTTTAATTACAGCGTTTTTATTGTTGT	147.32211
BB74040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82597	WGS84	BB74040	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74040_Cmae_Bot2011_COI	MA95	1	BB74040_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74040	Sample	BB74040	targeted	Absent	Sample	Cmae_Bot2011_COI	MA95	COI	45	BB74040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA95	ATGAACAGTCTATCCTCCTTTAG	151.24899
BB74040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82597	WGS84	BB74040	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74040_Msal_Bot2012_COI	MA95	1	BB74040_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74040	Sample	BB74040	targeted	Absent	Sample	Msal_Bot2012_COI	MA95	COI	45	BB74040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA95	GYTAGTTCCRATGATGTTAGCTG	151.24899
BB74042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82275	WGS84	BB74042	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.245	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74042_Aamu_Bax2006_COI	MA71	1	BB74042_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74042	Sample	BB74042	targeted	Present	Sample	Aamu_Bax2006_COI	MA71	COI	45	BB74042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA71	GCACAACCGGGATCTTTACTTC	144.90789
BB74042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82275	WGS84	BB74042	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74042_Pcan_Bot2011_IGS	MA71	1	BB74042_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74042	Sample	BB74042	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA71	IGS	45	BB74042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA71	CGTAATCCTCAGTACTGGCTA	144.90789
BB74042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82275	WGS84	BB74042	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74042_Sspa_Oph2007_COI	MA71	1	BB74042_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74042	Sample	BB74042	targeted	Absent	Sample	Sspa_Oph2007_COI	MA71	COI	45	BB74042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA71	GAAGGCCTTCCGATGATC	144.90789
BB74042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82275	WGS84	BB74042	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74042_Upin_Bot2011_COI	MA71	1	BB74042_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74042	Sample	BB74042	targeted	Absent	Sample	Upin_Bot2011_COI	MA71	COI	45	BB74042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA71	CTTTAATTACAGCGTTTTTATTGTTGT	144.90789
BB74043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82556	WGS84	BB74043	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74043_Mgig_Bot2012_COI	MA63	1	BB74043_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74043	Sample	BB74043	targeted	Absent	Sample	Mgig_Bot2012_COI	MA63	COI	45	BB74043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA63	TCTTATTCGTTGGAGACTTTATAACCCT	144.90337
BB74044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88449	WGS84	BB74044	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.206	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74044_Cmae_Bot2011_COI	HA10	1	BB74044_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74044	Sample	BB74044	targeted	Present	Sample	Cmae_Bot2011_COI	HA10	COI	45	BB74044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA10	ATGAACAGTCTATCCTCCTTTAG	147.33547
BB74044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88449	WGS84	BB74044	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74044_Msal_Bot2012_COI	HA10	1	BB74044_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74044	Sample	BB74044	targeted	Absent	Sample	Msal_Bot2012_COI	HA10	COI	45	BB74044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA10	GYTAGTTCCRATGATGTTAGCTG	147.33547
BB74046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81672	WGS84	BB74046	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74046_Aamu_Bax2006_COI	GA107	1	BB74046_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74046	Sample	BB74046	targeted	Absent	Sample	Aamu_Bax2006_COI	GA107	COI	45	BB74046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA107	GCACAACCGGGATCTTTACTTC	151.24042
BB74046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81672	WGS84	BB74046	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74046_Pcan_Bot2011_IGS	GA107	1	BB74046_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74046	Sample	BB74046	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA107	IGS	45	BB74046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA107	CGTAATCCTCAGTACTGGCTA	151.24042
BB74046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81672	WGS84	BB74046	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74046_Sspa_Oph2007_COI	GA107	1	BB74046_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74046	Sample	BB74046	targeted	Absent	Sample	Sspa_Oph2007_COI	GA107	COI	45	BB74046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA107	GAAGGCCTTCCGATGATC	151.24042
BB74046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81672	WGS84	BB74046	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74046_Upin_Bot2011_COI	GA107	1	BB74046_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74046	Sample	BB74046	targeted	Absent	Sample	Upin_Bot2011_COI	GA107	COI	45	BB74046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA107	CTTTAATTACAGCGTTTTTATTGTTGT	151.24042
BB74047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8179	WGS84	BB74047	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74047_Mgig_Bot2012_COI	GA106	1	BB74047_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74047	Sample	BB74047	targeted	Absent	Sample	Mgig_Bot2012_COI	GA106	COI	45	BB74047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA106	TCTTATTCGTTGGAGACTTTATAACCCT	151.24243
BB74048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82161	WGS84	BB74048	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74048_Cmae_Bot2011_COI	GA104	1	BB74048_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74048	Sample	BB74048	targeted	Absent	Sample	Cmae_Bot2011_COI	GA104	COI	45	BB74048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA104	ATGAACAGTCTATCCTCCTTTAG	151.24523
BB74048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82161	WGS84	BB74048	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74048_Msal_Bot2012_COI	GA104	1	BB74048_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74048	Sample	BB74048	targeted	Absent	Sample	Msal_Bot2012_COI	GA104	COI	45	BB74048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA104	GYTAGTTCCRATGATGTTAGCTG	151.24523
BB74050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81682	WGS84	BB74050	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74050_Mgig_Bot2012_COI	GA101	1	BB74050_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74050	Sample	BB74050	targeted	Absent	Sample	Mgig_Bot2012_COI	GA101	COI	45	BB74050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA101	TCTTATTCGTTGGAGACTTTATAACCCT	151.23872
BB74051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81866	WGS84	BB74051	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74051_Cmae_Bot2011_COI	GA99	1	BB74051_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74051	Sample	BB74051	targeted	Absent	Sample	Cmae_Bot2011_COI	GA99	COI	45	BB74051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA99	ATGAACAGTCTATCCTCCTTTAG	151.23785
BB74051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81866	WGS84	BB74051	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74051_Msal_Bot2012_COI	GA99	1	BB74051_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74051	Sample	BB74051	targeted	Absent	Sample	Msal_Bot2012_COI	GA99	COI	45	BB74051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA99	GYTAGTTCCRATGATGTTAGCTG	151.23785
BB74053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82761	WGS84	BB74053	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74053_Aamu_Bax2006_COI	GA97	1	BB74053_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74053	Sample	BB74053	targeted	Absent	Sample	Aamu_Bax2006_COI	GA97	COI	45	BB74053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA97	GCACAACCGGGATCTTTACTTC	151.24723
BB74053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82761	WGS84	BB74053	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74053_Pcan_Bot2011_IGS	GA97	1	BB74053_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74053	Sample	BB74053	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA97	IGS	45	BB74053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA97	CGTAATCCTCAGTACTGGCTA	151.24723
BB74053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82761	WGS84	BB74053	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74053_Sspa_Oph2007_COI	GA97	1	BB74053_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74053	Sample	BB74053	targeted	Absent	Sample	Sspa_Oph2007_COI	GA97	COI	45	BB74053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA97	GAAGGCCTTCCGATGATC	151.24723
BB74053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82761	WGS84	BB74053	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74053_Upin_Bot2011_COI	GA97	1	BB74053_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74053	Sample	BB74053	targeted	Absent	Sample	Upin_Bot2011_COI	GA97	COI	45	BB74053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA97	CTTTAATTACAGCGTTTTTATTGTTGT	151.24723
BB74054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82638	WGS84	BB74054	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74054_Mgig_Bot2012_COI	GA96	1	BB74054_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74054	Sample	BB74054	targeted	Absent	Sample	Mgig_Bot2012_COI	GA96	COI	45	BB74054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA96	TCTTATTCGTTGGAGACTTTATAACCCT	151.24668
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74055_Cmae_Bot2011_COI	MA118	1	BB74055_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Absent	Sample	Cmae_Bot2011_COI	MA118	COI	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	ATGAACAGTCTATCCTCCTTTAG	144.92535
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74055_Hsan_Wil2021_COI	MA118	1	BB74055_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Absent	Sample	Hsan_Wil2021_COI	MA118	COI	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	CCTGGGCCGGTATAGTAGGT	144.92535
BB74055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81611	WGS84	BB74055	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74055_Msal_Bot2012_COI	MA118	1	BB74055_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74055	Sample	BB74055	targeted	Absent	Sample	Msal_Bot2012_COI	MA118	COI	45	BB74055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA118	GYTAGTTCCRATGATGTTAGCTG	144.92535
BB74057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82383	WGS84	BB74057	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74057_Aamu_Bax2006_COI	GA92	1	BB74057_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74057	Sample	BB74057	targeted	Absent	Sample	Aamu_Bax2006_COI	GA92	COI	45	BB74057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA92	GCACAACCGGGATCTTTACTTC	151.24413
BB74057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82383	WGS84	BB74057	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74057_Pcan_Bot2011_IGS	GA92	1	BB74057_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74057	Sample	BB74057	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA92	IGS	45	BB74057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA92	CGTAATCCTCAGTACTGGCTA	151.24413
BB74057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82383	WGS84	BB74057	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74057_Sspa_Oph2007_COI	GA92	1	BB74057_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74057	Sample	BB74057	targeted	Absent	Sample	Sspa_Oph2007_COI	GA92	COI	45	BB74057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA92	GAAGGCCTTCCGATGATC	151.24413
BB74057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82383	WGS84	BB74057	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74057_Upin_Bot2011_COI	GA92	1	BB74057_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74057	Sample	BB74057	targeted	Absent	Sample	Upin_Bot2011_COI	GA92	COI	45	BB74057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA92	CTTTAATTACAGCGTTTTTATTGTTGT	151.24413
BB74058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82347	WGS84	BB74058	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74058_Mgig_Bot2012_COI	GA91	1	BB74058_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74058	Sample	BB74058	targeted	Absent	Sample	Mgig_Bot2012_COI	GA91	COI	45	BB74058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA91	TCTTATTCGTTGGAGACTTTATAACCCT	151.24428
BB74059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44339	WGS84	BB74059	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74059_Cmae_Bot2011_COI	BA1	1	BB74059_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74059	Sample	BB74059	targeted	Absent	Sample	Cmae_Bot2011_COI	BA1	COI	45	BB74059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA1	ATGAACAGTCTATCCTCCTTTAG	153.07218
BB74059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44339	WGS84	BB74059	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74059_Msal_Bot2012_COI	BA1	1	BB74059_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74059	Sample	BB74059	targeted	Absent	Sample	Msal_Bot2012_COI	BA1	COI	45	BB74059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA1	GYTAGTTCCRATGATGTTAGCTG	153.07218
BB74060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82381	WGS84	BB74060	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74060_Aamu_Bax2006_COI	GA88	1	BB74060_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74060	Sample	BB74060	targeted	Absent	Sample	Aamu_Bax2006_COI	GA88	COI	45	BB74060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA88	GCACAACCGGGATCTTTACTTC	151.24304
BB74060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82381	WGS84	BB74060	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74060_Pcan_Bot2011_IGS	GA88	1	BB74060_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74060	Sample	BB74060	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA88	IGS	45	BB74060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA88	CGTAATCCTCAGTACTGGCTA	151.24304
BB74060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82381	WGS84	BB74060	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74060_Sspa_Oph2007_COI	GA88	1	BB74060_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74060	Sample	BB74060	targeted	Absent	Sample	Sspa_Oph2007_COI	GA88	COI	45	BB74060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA88	GAAGGCCTTCCGATGATC	151.24304
BB74060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82381	WGS84	BB74060	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74060_Upin_Bot2011_COI	GA88	1	BB74060_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74060	Sample	BB74060	targeted	Absent	Sample	Upin_Bot2011_COI	GA88	COI	45	BB74060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA88	CTTTAATTACAGCGTTTTTATTGTTGT	151.24304
BB74061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82289	WGS84	BB74061	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74061_Mgig_Bot2012_COI	GA87	1	BB74061_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74061	Sample	BB74061	targeted	Absent	Sample	Mgig_Bot2012_COI	GA87	COI	45	BB74061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA87	TCTTATTCGTTGGAGACTTTATAACCCT	151.24174
BB74062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82186	WGS84	BB74062	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74062_Cmae_Bot2011_COI	GA86	1	BB74062_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74062	Sample	BB74062	targeted	Absent	Sample	Cmae_Bot2011_COI	GA86	COI	45	BB74062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA86	ATGAACAGTCTATCCTCCTTTAG	151.24211
BB74062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82186	WGS84	BB74062	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74062_Msal_Bot2012_COI	GA86	1	BB74062_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74062	Sample	BB74062	targeted	Absent	Sample	Msal_Bot2012_COI	GA86	COI	45	BB74062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA86	GYTAGTTCCRATGATGTTAGCTG	151.24211
BB74064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82104	WGS84	BB74064	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74064_Aamu_Bax2006_COI	GA83	1	BB74064_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74064	Sample	BB74064	targeted	Absent	Sample	Aamu_Bax2006_COI	GA83	COI	45	BB74064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA83	GCACAACCGGGATCTTTACTTC	151.24161
BB74064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82104	WGS84	BB74064	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74064_Pcan_Bot2011_IGS	GA83	1	BB74064_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74064	Sample	BB74064	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA83	IGS	45	BB74064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA83	CGTAATCCTCAGTACTGGCTA	151.24161
BB74064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82104	WGS84	BB74064	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74064_Sspa_Oph2007_COI	GA83	1	BB74064_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74064	Sample	BB74064	targeted	Absent	Sample	Sspa_Oph2007_COI	GA83	COI	45	BB74064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA83	GAAGGCCTTCCGATGATC	151.24161
BB74064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82104	WGS84	BB74064	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74064_Upin_Bot2011_COI	GA83	1	BB74064_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74064	Sample	BB74064	targeted	Absent	Sample	Upin_Bot2011_COI	GA83	COI	45	BB74064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA83	CTTTAATTACAGCGTTTTTATTGTTGT	151.24161
BB74065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82039	WGS84	BB74065	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74065_Mgig_Bot2012_COI	GA82	1	BB74065_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74065	Sample	BB74065	targeted	Absent	Sample	Mgig_Bot2012_COI	GA82	COI	45	BB74065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA82	TCTTATTCGTTGGAGACTTTATAACCCT	151.24193
BB74066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82005	WGS84	BB74066	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74066_Cmae_Bot2011_COI	GA81	1	BB74066_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74066	Sample	BB74066	targeted	Absent	Sample	Cmae_Bot2011_COI	GA81	COI	45	BB74066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA81	ATGAACAGTCTATCCTCCTTTAG	151.24155
BB74066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82005	WGS84	BB74066	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74066_Msal_Bot2012_COI	GA81	1	BB74066_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74066	Sample	BB74066	targeted	Absent	Sample	Msal_Bot2012_COI	GA81	COI	45	BB74066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA81	GYTAGTTCCRATGATGTTAGCTG	151.24155
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.77	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74068_Aamu_Bax2006_COI	MA69	1	BB74068_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Present	Sample	Aamu_Bax2006_COI	MA69	COI	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	GCACAACCGGGATCTTTACTTC	144.90659
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74068_Pcan_Bot2011_IGS	MA69	1	BB74068_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA69	IGS	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	CGTAATCCTCAGTACTGGCTA	144.90659
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.126	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74068_Sspa_Oph2007_COI	MA69	1	BB74068_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Present	Sample	Sspa_Oph2007_COI	MA69	COI	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	GAAGGCCTTCCGATGATC	144.90659
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74068_Upin_Bot2011_COI	MA69	1	BB74068_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Absent	Sample	Upin_Bot2011_COI	MA69	COI	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	CTTTAATTACAGCGTTTTTATTGTTGT	144.90659
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74069_Mgig_Bot2012_COI	MA72	1	BB74069_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Absent	Sample	Mgig_Bot2012_COI	MA72	COI	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	TCTTATTCGTTGGAGACTTTATAACCCT	144.90996
BB74071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81913	WGS84	BB74071	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74071_Aamu_Bax2006_COI	GA18	1	BB74071_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74071	Sample	BB74071	targeted	Absent	Sample	Aamu_Bax2006_COI	GA18	COI	45	BB74071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA18	GCACAACCGGGATCTTTACTTC	151.23931
BB74071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81913	WGS84	BB74071	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74071_Pcan_Bot2011_IGS	GA18	1	BB74071_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74071	Sample	BB74071	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA18	IGS	45	BB74071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA18	CGTAATCCTCAGTACTGGCTA	151.23931
BB74071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81913	WGS84	BB74071	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74071_Sspa_Oph2007_COI	GA18	1	BB74071_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74071	Sample	BB74071	targeted	Absent	Sample	Sspa_Oph2007_COI	GA18	COI	45	BB74071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA18	GAAGGCCTTCCGATGATC	151.23931
BB74071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81913	WGS84	BB74071	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74071_Upin_Bot2011_COI	GA18	1	BB74071_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74071	Sample	BB74071	targeted	Absent	Sample	Upin_Bot2011_COI	GA18	COI	45	BB74071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA18	CTTTAATTACAGCGTTTTTATTGTTGT	151.23931
BB74072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81501	WGS84	BB74072	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74072_Mgig_Bot2012_COI	GA17	1	BB74072_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74072	Sample	BB74072	targeted	Absent	Sample	Mgig_Bot2012_COI	GA17	COI	45	BB74072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA17	TCTTATTCGTTGGAGACTTTATAACCCT	151.23683
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74073_Cmae_Bot2011_COI	MA54	1	BB74073_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Absent	Sample	Cmae_Bot2011_COI	MA54	COI	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	ATGAACAGTCTATCCTCCTTTAG	144.92822
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74073_Hsan_Wil2021_COI	MA54	1	BB74073_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Absent	Sample	Hsan_Wil2021_COI	MA54	COI	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	CCTGGGCCGGTATAGTAGGT	144.92822
BB74073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8202	WGS84	BB74073	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74073_Msal_Bot2012_COI	MA54	1	BB74073_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74073	Sample	BB74073	targeted	Absent	Sample	Msal_Bot2012_COI	MA54	COI	45	BB74073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA54	GYTAGTTCCRATGATGTTAGCTG	144.92822
BB74075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81598	WGS84	BB74075	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74075_Aamu_Bax2006_COI	GA13	1	BB74075_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74075	Sample	BB74075	targeted	Absent	Sample	Aamu_Bax2006_COI	GA13	COI	45	BB74075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA13	GCACAACCGGGATCTTTACTTC	151.23583
BB74075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81598	WGS84	BB74075	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74075_Pcan_Bot2011_IGS	GA13	1	BB74075_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74075	Sample	BB74075	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA13	IGS	45	BB74075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA13	CGTAATCCTCAGTACTGGCTA	151.23583
BB74075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81598	WGS84	BB74075	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74075_Sspa_Oph2007_COI	GA13	1	BB74075_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74075	Sample	BB74075	targeted	Absent	Sample	Sspa_Oph2007_COI	GA13	COI	45	BB74075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA13	GAAGGCCTTCCGATGATC	151.23583
BB74075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81598	WGS84	BB74075	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74075_Upin_Bot2011_COI	GA13	1	BB74075_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74075	Sample	BB74075	targeted	Absent	Sample	Upin_Bot2011_COI	GA13	COI	45	BB74075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA13	CTTTAATTACAGCGTTTTTATTGTTGT	151.23583
BB74076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83155	WGS84	BB74076	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74076_Mgig_Bot2012_COI	MA75	1	BB74076_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74076	Sample	BB74076	targeted	Absent	Sample	Mgig_Bot2012_COI	MA75	COI	45	BB74076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA75	TCTTATTCGTTGGAGACTTTATAACCCT	151.25742
BB74077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83422	WGS84	BB74077	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74077_Cmae_Bot2011_COI	MA40	1	BB74077_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74077	Sample	BB74077	targeted	Absent	Sample	Cmae_Bot2011_COI	MA40	COI	45	BB74077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA40	ATGAACAGTCTATCCTCCTTTAG	151.27119
BB74077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83422	WGS84	BB74077	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74077_Msal_Bot2012_COI	MA40	1	BB74077_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74077	Sample	BB74077	targeted	Absent	Sample	Msal_Bot2012_COI	MA40	COI	45	BB74077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA40	GYTAGTTCCRATGATGTTAGCTG	151.27119
BB74079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83171	WGS84	BB74079	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74079_Aamu_Bax2006_COI	GA77	1	BB74079_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74079	Sample	BB74079	targeted	Absent	Sample	Aamu_Bax2006_COI	GA77	COI	45	BB74079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA77	GCACAACCGGGATCTTTACTTC	151.262
BB74079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83171	WGS84	BB74079	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74079_Pcan_Bot2011_IGS	GA77	1	BB74079_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74079	Sample	BB74079	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA77	IGS	45	BB74079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA77	CGTAATCCTCAGTACTGGCTA	151.262
BB74079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83171	WGS84	BB74079	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74079_Sspa_Oph2007_COI	GA77	1	BB74079_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74079	Sample	BB74079	targeted	Absent	Sample	Sspa_Oph2007_COI	GA77	COI	45	BB74079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA77	GAAGGCCTTCCGATGATC	151.262
BB74079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83171	WGS84	BB74079	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74079_Upin_Bot2011_COI	GA77	1	BB74079_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74079	Sample	BB74079	targeted	Absent	Sample	Upin_Bot2011_COI	GA77	COI	45	BB74079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA77	CTTTAATTACAGCGTTTTTATTGTTGT	151.262
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.268	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74080_Cmae_Bot2011_COI	MA104	1	BB74080_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Present	Sample	Cmae_Bot2011_COI	MA104	COI	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	ATGAACAGTCTATCCTCCTTTAG	144.90901
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74080_Hsan_Wil2021_COI	MA104	1	BB74080_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Absent	Sample	Hsan_Wil2021_COI	MA104	COI	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	CCTGGGCCGGTATAGTAGGT	144.90901
BB74080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82058	WGS84	BB74080	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74080_Msal_Bot2012_COI	MA104	1	BB74080_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74080	Sample	BB74080	targeted	Absent	Sample	Msal_Bot2012_COI	MA104	COI	45	BB74080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA104	GYTAGTTCCRATGATGTTAGCTG	144.90901
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.048	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74082_Aamu_Bax2006_COI	MA106	1	BB74082_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Present	Sample	Aamu_Bax2006_COI	MA106	COI	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	GCACAACCGGGATCTTTACTTC	144.91161
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74082_Pcan_Bot2011_IGS	MA106	1	BB74082_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA106	IGS	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	CGTAATCCTCAGTACTGGCTA	144.91161
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.693	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74082_Sspa_Oph2007_COI	MA106	1	BB74082_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Present	Sample	Sspa_Oph2007_COI	MA106	COI	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	GAAGGCCTTCCGATGATC	144.91161
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74082_Upin_Bot2011_COI	MA106	1	BB74082_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Absent	Sample	Upin_Bot2011_COI	MA106	COI	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	CTTTAATTACAGCGTTTTTATTGTTGT	144.91161
BB74083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83492	WGS84	BB74083	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74083_Mgig_Bot2012_COI	GA43	1	BB74083_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74083	Sample	BB74083	targeted	Absent	Sample	Mgig_Bot2012_COI	GA43	COI	45	BB74083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA43	TCTTATTCGTTGGAGACTTTATAACCCT	151.26834
BB74084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82352	WGS84	BB74084	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74084_Cmae_Bot2011_COI	MA107	1	BB74084_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74084	Sample	BB74084	targeted	Absent	Sample	Cmae_Bot2011_COI	MA107	COI	45	BB74084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA107	ATGAACAGTCTATCCTCCTTTAG	144.90269
BB74084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82352	WGS84	BB74084	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74084_Msal_Bot2012_COI	MA107	1	BB74084_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74084	Sample	BB74084	targeted	Absent	Sample	Msal_Bot2012_COI	MA107	COI	45	BB74084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA107	GYTAGTTCCRATGATGTTAGCTG	144.90269
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.894	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74086_Aamu_Bax2006_COI	MA53	1	BB74086_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Present	Sample	Aamu_Bax2006_COI	MA53	COI	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	GCACAACCGGGATCTTTACTTC	144.92399
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74086_Pcan_Bot2011_IGS	MA53	1	BB74086_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA53	IGS	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	CGTAATCCTCAGTACTGGCTA	144.92399
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74086_Sspa_Oph2007_COI	MA53	1	BB74086_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Absent	Sample	Sspa_Oph2007_COI	MA53	COI	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	GAAGGCCTTCCGATGATC	144.92399
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74086_Upin_Bot2011_COI	MA53	1	BB74086_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Absent	Sample	Upin_Bot2011_COI	MA53	COI	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	CTTTAATTACAGCGTTTTTATTGTTGT	144.92399
BB74087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8363	WGS84	BB74087	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74087_Mgig_Bot2012_COI	GA38	1	BB74087_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74087	Sample	BB74087	targeted	Absent	Sample	Mgig_Bot2012_COI	GA38	COI	45	BB74087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA38	TCTTATTCGTTGGAGACTTTATAACCCT	151.27496
BB74088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83619	WGS84	BB74088	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74088_Cmae_Bot2011_COI	GA37	1	BB74088_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74088	Sample	BB74088	targeted	Absent	Sample	Cmae_Bot2011_COI	GA37	COI	45	BB74088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA37	ATGAACAGTCTATCCTCCTTTAG	151.2719
BB74088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83619	WGS84	BB74088	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74088_Msal_Bot2012_COI	GA37	1	BB74088_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74088	Sample	BB74088	targeted	Absent	Sample	Msal_Bot2012_COI	GA37	COI	45	BB74088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA37	GYTAGTTCCRATGATGTTAGCTG	151.2719
BB74187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85244	WGS84	BB74187	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74187_Cmae_Bot2011_COI	T1-01	1	BB74187_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74187	Sample	BB74187	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-01	COI	45	BB74187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-01	ATGAACAGTCTATCCTCCTTTAG	147.86938
BB74189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85804	WGS84	BB74189	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74189_Aamu_Bax2006_COI	T1-17	1	BB74189_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74189	Sample	BB74189	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-17	COI	45	BB74189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-17	GCACAACCGGGATCTTTACTTC	147.86795
BB74189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85804	WGS84	BB74189	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74189_Pcan_Bot2011_IGS	T1-17	1	BB74189_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74189	Sample	BB74189	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-17	IGS	45	BB74189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-17	CGTAATCCTCAGTACTGGCTA	147.86795
BB74189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85804	WGS84	BB74189	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74189_Sspa_Oph2007_COI	T1-17	1	BB74189_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74189	Sample	BB74189	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-17	COI	45	BB74189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-17	GAAGGCCTTCCGATGATC	147.86795
BB74189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85804	WGS84	BB74189	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74189_Upin_Bot2011_COI	T1-17	1	BB74189_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74189	Sample	BB74189	targeted	Absent	Sample	Upin_Bot2011_COI	T1-17	COI	45	BB74189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-17	CTTTAATTACAGCGTTTTTATTGTTGT	147.86795
BB74190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95973	WGS84	BB74190	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74190_Cmae_Bot2011_COI	T1-02	1	BB74190_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74190	Sample	BB74190	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-02	COI	45	BB74190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-02	ATGAACAGTCTATCCTCCTTTAG	147.52492
BB74192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96019	WGS84	BB74192	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.276	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74192_Aamu_Bax2006_COI	T1-18	1	BB74192_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74192	Sample	BB74192	targeted	Present	Sample	Aamu_Bax2006_COI	T1-18	COI	45	BB74192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-18	GCACAACCGGGATCTTTACTTC	147.52897
BB74192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96019	WGS84	BB74192	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74192_Pcan_Bot2011_IGS	T1-18	1	BB74192_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74192	Sample	BB74192	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-18	IGS	45	BB74192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-18	CGTAATCCTCAGTACTGGCTA	147.52897
BB74192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96019	WGS84	BB74192	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74192_Sspa_Oph2007_COI	T1-18	1	BB74192_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74192	Sample	BB74192	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-18	COI	45	BB74192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-18	GAAGGCCTTCCGATGATC	147.52897
BB74192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96019	WGS84	BB74192	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74192_Upin_Bot2011_COI	T1-18	1	BB74192_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74192	Sample	BB74192	targeted	Absent	Sample	Upin_Bot2011_COI	T1-18	COI	45	BB74192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-18	CTTTAATTACAGCGTTTTTATTGTTGT	147.52897
BB74193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88525	WGS84	BB74193	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74193_Cjap_Sim2018_COI	T1-03	1	BB74193_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74193	Sample	BB74193	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-03	COI	45	BB74193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3358
BB74193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88525	WGS84	BB74193	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.794	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74193_Mgig_Bot2012_COI	T1-03	1	BB74193_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74193	Sample	BB74193	targeted	Present	Sample	Mgig_Bot2012_COI	T1-03	COI	45	BB74193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.3358
BB74194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88441	WGS84	BB74194	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74194_Cmae_Bot2011_COI	T1-11	1	BB74194_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74194	Sample	BB74194	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-11	COI	45	BB74194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-11	ATGAACAGTCTATCCTCCTTTAG	147.33665
BB74196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87764	WGS84	BB74196	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74196_Aamu_Bax2006_COI	T1-04	1	BB74196_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74196	Sample	BB74196	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-04	COI	45	BB74196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-04	GCACAACCGGGATCTTTACTTC	147.34464
BB74196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87764	WGS84	BB74196	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74196_Pcan_Bot2011_IGS	T1-04	1	BB74196_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74196	Sample	BB74196	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-04	IGS	45	BB74196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-04	CGTAATCCTCAGTACTGGCTA	147.34464
BB74196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87764	WGS84	BB74196	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74196_Sspa_Oph2007_COI	T1-04	1	BB74196_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74196	Sample	BB74196	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-04	COI	45	BB74196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-04	GAAGGCCTTCCGATGATC	147.34464
BB74196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87764	WGS84	BB74196	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74196_Upin_Bot2011_COI	T1-04	1	BB74196_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74196	Sample	BB74196	targeted	Absent	Sample	Upin_Bot2011_COI	T1-04	COI	45	BB74196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.34464
BB74197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82601	WGS84	BB74197	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74197_Cjap_Sim2018_COI	T1-12	1	BB74197_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74197	Sample	BB74197	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-12	COI	45	BB74197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30451
BB74197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82601	WGS84	BB74197	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.677	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74197_Mgig_Bot2012_COI	T1-12	1	BB74197_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74197	Sample	BB74197	targeted	Present	Sample	Mgig_Bot2012_COI	T1-12	COI	45	BB74197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.30451
BB74198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82426	WGS84	BB74198	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74198_Cmae_Bot2011_COI	T1-20	1	BB74198_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74198	Sample	BB74198	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-20	COI	45	BB74198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-20	ATGAACAGTCTATCCTCCTTTAG	147.30787
BB74200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84483	WGS84	BB74200	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74200_Aamu_Bax2006_COI	S2-05	1	BB74200_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74200	Sample	BB74200	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-05	COI	45	BB74200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-05	GCACAACCGGGATCTTTACTTC	138.49685
BB74200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84483	WGS84	BB74200	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74200_Pcan_Bot2011_IGS	S2-05	1	BB74200_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74200	Sample	BB74200	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-05	IGS	45	BB74200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-05	CGTAATCCTCAGTACTGGCTA	138.49685
BB74200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84483	WGS84	BB74200	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.095	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74200_Sspa_Oph2007_COI	S2-05	1	BB74200_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74200	Sample	BB74200	targeted	Present	Sample	Sspa_Oph2007_COI	S2-05	COI	45	BB74200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-05	GAAGGCCTTCCGATGATC	138.49685
BB74200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84483	WGS84	BB74200	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74200_Upin_Bot2011_COI	S2-05	1	BB74200_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74200	Sample	BB74200	targeted	Absent	Sample	Upin_Bot2011_COI	S2-05	COI	45	BB74200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.49685
BB74201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83684	WGS84	BB74201	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74201_Cjap_Sim2018_COI	S2-08	1	BB74201_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74201	Sample	BB74201	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-08	COI	45	BB74201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50934
BB74201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83684	WGS84	BB74201	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74201_Mgig_Bot2012_COI	S2-08	1	BB74201_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74201	Sample	BB74201	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-08	COI	45	BB74201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.50934
BB74202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78745	WGS84	BB74202	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74202_Cmae_Bot2011_COI	S2-11	1	BB74202_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74202	Sample	BB74202	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-11	COI	45	BB74202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-11	ATGAACAGTCTATCCTCCTTTAG	138.51416
BB74204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78612	WGS84	BB74204	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74204_Aamu_Bax2006_COI	S2-13	1	BB74204_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74204	Sample	BB74204	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-13	COI	45	BB74204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-13	GCACAACCGGGATCTTTACTTC	138.51493
BB74204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78612	WGS84	BB74204	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74204_Pcan_Bot2011_IGS	S2-13	1	BB74204_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74204	Sample	BB74204	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-13	IGS	45	BB74204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-13	CGTAATCCTCAGTACTGGCTA	138.51493
BB74204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78612	WGS84	BB74204	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.84	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74204_Sspa_Oph2007_COI	S2-13	1	BB74204_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74204	Sample	BB74204	targeted	Present	Sample	Sspa_Oph2007_COI	S2-13	COI	45	BB74204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-13	GAAGGCCTTCCGATGATC	138.51493
BB74204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78612	WGS84	BB74204	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74204_Upin_Bot2011_COI	S2-13	1	BB74204_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74204	Sample	BB74204	targeted	Absent	Sample	Upin_Bot2011_COI	S2-13	COI	45	BB74204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.51493
BB74205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80954	WGS84	BB74205	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74205_Cjap_Sim2018_COI	S2-19	1	BB74205_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74205	Sample	BB74205	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-19	COI	45	BB74205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51877
BB74205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80954	WGS84	BB74205	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74205_Mgig_Bot2012_COI	S2-19	1	BB74205_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74205	Sample	BB74205	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-19	COI	45	BB74205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.51877
BB74206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78616	WGS84	BB74206	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.503	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74206_Cmae_Bot2011_COI	S2-14	1	BB74206_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74206	Sample	BB74206	targeted	Present	Sample	Cmae_Bot2011_COI	S2-14	COI	45	BB74206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-14	ATGAACAGTCTATCCTCCTTTAG	138.51483
BB75216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78716	WGS84	BB75216	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75216_Aamu_Bax2006_COI	S2-15	1	BB75216_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75216	Sample	BB75216	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-15	COI	45	BB75216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-15	GCACAACCGGGATCTTTACTTC	138.51476
BB75216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78716	WGS84	BB75216	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75216_Pcan_Bot2011_IGS	S2-15	1	BB75216_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75216	Sample	BB75216	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-15	IGS	45	BB75216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-15	CGTAATCCTCAGTACTGGCTA	138.51476
BB75216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78716	WGS84	BB75216	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.096	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75216_Sspa_Oph2007_COI	S2-15	1	BB75216_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75216	Sample	BB75216	targeted	Present	Sample	Sspa_Oph2007_COI	S2-15	COI	45	BB75216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-15	GAAGGCCTTCCGATGATC	138.51476
BB75216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78716	WGS84	BB75216	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75216_Upin_Bot2011_COI	S2-15	1	BB75216_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75216	Sample	BB75216	targeted	Absent	Sample	Upin_Bot2011_COI	S2-15	COI	45	BB75216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-15	CTTTAATTACAGCGTTTTTATTGTTGT	138.51476
BB75217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80872	WGS84	BB75217	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75217_Cjap_Sim2018_COI	S2-16	1	BB75217_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75217	Sample	BB75217	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-16	COI	45	BB75217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51653
BB75217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80872	WGS84	BB75217	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75217_Mgig_Bot2012_COI	S2-16	1	BB75217_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75217	Sample	BB75217	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-16	COI	45	BB75217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.51653
BB75218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80908	WGS84	BB75218	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75218_Cmae_Bot2011_COI	S2-17	1	BB75218_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75218	Sample	BB75218	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-17	COI	45	BB75218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-17	ATGAACAGTCTATCCTCCTTTAG	138.51763
BB75221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85207	WGS84	BB75221	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75221_Cmae_Bot2011_COI	T1-05	1	BB75221_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75221	Sample	BB75221	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-05	COI	45	BB75221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-05	ATGAACAGTCTATCCTCCTTTAG	147.87165
BB75223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96422	WGS84	BB75223	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.89	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75223_Aamu_Bax2006_COI	T1-06	1	BB75223_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75223	Sample	BB75223	targeted	Present	Sample	Aamu_Bax2006_COI	T1-06	COI	45	BB75223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-06	GCACAACCGGGATCTTTACTTC	147.51968
BB75223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96422	WGS84	BB75223	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75223_Pcan_Bot2011_IGS	T1-06	1	BB75223_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75223	Sample	BB75223	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-06	IGS	45	BB75223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-06	CGTAATCCTCAGTACTGGCTA	147.51968
BB75223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96422	WGS84	BB75223	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75223_Sspa_Oph2007_COI	T1-06	1	BB75223_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75223	Sample	BB75223	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-06	COI	45	BB75223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-06	GAAGGCCTTCCGATGATC	147.51968
BB75223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96422	WGS84	BB75223	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75223_Upin_Bot2011_COI	T1-06	1	BB75223_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75223	Sample	BB75223	targeted	Absent	Sample	Upin_Bot2011_COI	T1-06	COI	45	BB75223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.51968
BB75225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88573	WGS84	BB75225	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75225_Cmae_Bot2011_COI	T1-07	1	BB75225_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75225	Sample	BB75225	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-07	COI	45	BB75225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-07	ATGAACAGTCTATCCTCCTTTAG	147.33828
BB75227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87996	WGS84	BB75227	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75227_Aamu_Bax2006_COI	T1-08	1	BB75227_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75227	Sample	BB75227	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-08	COI	45	BB75227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-08	GCACAACCGGGATCTTTACTTC	147.34157
BB75227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87996	WGS84	BB75227	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75227_Pcan_Bot2011_IGS	T1-08	1	BB75227_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75227	Sample	BB75227	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-08	IGS	45	BB75227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-08	CGTAATCCTCAGTACTGGCTA	147.34157
BB75227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87996	WGS84	BB75227	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75227_Sspa_Oph2007_COI	T1-08	1	BB75227_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75227	Sample	BB75227	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-08	COI	45	BB75227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-08	GAAGGCCTTCCGATGATC	147.34157
BB75227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87996	WGS84	BB75227	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75227_Upin_Bot2011_COI	T1-08	1	BB75227_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75227	Sample	BB75227	targeted	Absent	Sample	Upin_Bot2011_COI	T1-08	COI	45	BB75227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.34157
BB75228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82735	WGS84	BB75228	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75228_Cjap_Sim2018_COI	T1-16	1	BB75228_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75228	Sample	BB75228	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-16	COI	45	BB75228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3021
BB75228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82735	WGS84	BB75228	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.724	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75228_Mgig_Bot2012_COI	T1-16	1	BB75228_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75228	Sample	BB75228	targeted	Present	Sample	Mgig_Bot2012_COI	T1-16	COI	45	BB75228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.3021
BB75229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82712	WGS84	BB75229	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.027	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75229_Cmae_Bot2011_COI	T4-02	1	BB75229_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75229	Sample	BB75229	targeted	Present	Sample	Cmae_Bot2011_COI	T4-02	COI	45	BB75229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-02	ATGAACAGTCTATCCTCCTTTAG	147.3019
BB75230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87299	WGS84	BB75230	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.425	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75230_Aamu_Bax2006_COI	T4-04	1	BB75230_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75230	Sample	BB75230	targeted	Present	Sample	Aamu_Bax2006_COI	T4-04	COI	45	BB75230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-04	GCACAACCGGGATCTTTACTTC	147.33679
BB75230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87299	WGS84	BB75230	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75230_Pcan_Bot2011_IGS	T4-04	1	BB75230_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75230	Sample	BB75230	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-04	IGS	45	BB75230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-04	CGTAATCCTCAGTACTGGCTA	147.33679
BB75230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87299	WGS84	BB75230	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75230_Sspa_Oph2007_COI	T4-04	1	BB75230_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75230	Sample	BB75230	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-04	COI	45	BB75230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-04	GAAGGCCTTCCGATGATC	147.33679
BB75230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87299	WGS84	BB75230	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75230_Upin_Bot2011_COI	T4-04	1	BB75230_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75230	Sample	BB75230	targeted	Absent	Sample	Upin_Bot2011_COI	T4-04	COI	45	BB75230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.33679
BB75231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89813	WGS84	BB75231	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75231_Cjap_Sim2018_COI	T4-06	1	BB75231_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75231	Sample	BB75231	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-06	COI	45	BB75231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34208
BB75231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.89813	WGS84	BB75231	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75231_Mgig_Bot2012_COI	T4-06	1	BB75231_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75231	Sample	BB75231	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-06	COI	45	BB75231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.34208
BB75232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89955	WGS84	BB75232	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75232_Cmae_Bot2011_COI	T4-08	1	BB75232_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75232	Sample	BB75232	targeted	Absent	Sample	Cmae_Bot2011_COI	T4-08	COI	45	BB75232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-08	ATGAACAGTCTATCCTCCTTTAG	147.33941
BB75234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85713	WGS84	BB75234	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.61	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75234_Aamu_Bax2006_COI	T4-12	1	BB75234_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75234	Sample	BB75234	targeted	Present	Sample	Aamu_Bax2006_COI	T4-12	COI	45	BB75234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-12	GCACAACCGGGATCTTTACTTC	147.87276
BB75234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85713	WGS84	BB75234	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75234_Pcan_Bot2011_IGS	T4-12	1	BB75234_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75234	Sample	BB75234	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-12	IGS	45	BB75234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-12	CGTAATCCTCAGTACTGGCTA	147.87276
BB75234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85713	WGS84	BB75234	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75234_Sspa_Oph2007_COI	T4-12	1	BB75234_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75234	Sample	BB75234	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-12	COI	45	BB75234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-12	GAAGGCCTTCCGATGATC	147.87276
BB75234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85713	WGS84	BB75234	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.571	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75234_Upin_Bot2011_COI	T4-12	1	BB75234_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75234	Sample	BB75234	targeted	Present	Sample	Upin_Bot2011_COI	T4-12	COI	45	BB75234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.87276
BB75235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95924	WGS84	BB75235	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75235_Cjap_Sim2018_COI	T4-14	1	BB75235_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75235	Sample	BB75235	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-14	COI	45	BB75235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52587
BB75235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.95924	WGS84	BB75235	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75235_Mgig_Bot2012_COI	T4-14	1	BB75235_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75235	Sample	BB75235	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-14	COI	45	BB75235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.52587
BB75236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82928	WGS84	BB75236	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.85	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75236_Cmae_Bot2011_COI	T4-01	1	BB75236_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75236	Sample	BB75236	targeted	Present	Sample	Cmae_Bot2011_COI	T4-01	COI	45	BB75236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-01	ATGAACAGTCTATCCTCCTTTAG	147.30265
BB75238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87854	WGS84	BB75238	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.153	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75238_Aamu_Bax2006_COI	T4-05	1	BB75238_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75238	Sample	BB75238	targeted	Present	Sample	Aamu_Bax2006_COI	T4-05	COI	45	BB75238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-05	GCACAACCGGGATCTTTACTTC	147.34183
BB75238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87854	WGS84	BB75238	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75238_Pcan_Bot2011_IGS	T4-05	1	BB75238_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75238	Sample	BB75238	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-05	IGS	45	BB75238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-05	CGTAATCCTCAGTACTGGCTA	147.34183
BB75238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87854	WGS84	BB75238	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75238_Sspa_Oph2007_COI	T4-05	1	BB75238_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75238	Sample	BB75238	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-05	COI	45	BB75238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-05	GAAGGCCTTCCGATGATC	147.34183
BB75238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87854	WGS84	BB75238	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75238_Upin_Bot2011_COI	T4-05	1	BB75238_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75238	Sample	BB75238	targeted	Absent	Sample	Upin_Bot2011_COI	T4-05	COI	45	BB75238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.34183
BB75239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89951	WGS84	BB75239	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75239_Cjap_Sim2018_COI	T4-07	1	BB75239_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75239	Sample	BB75239	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-07	COI	45	BB75239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33637
BB75239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.89951	WGS84	BB75239	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75239_Mgig_Bot2012_COI	T4-07	1	BB75239_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75239	Sample	BB75239	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-07	COI	45	BB75239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.33637
BB75241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.8512	WGS84	BB75241	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75241_Aamu_Bax2006_COI	T4-11	1	BB75241_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75241	Sample	BB75241	targeted	Absent	Sample	Aamu_Bax2006_COI	T4-11	COI	45	BB75241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-11	GCACAACCGGGATCTTTACTTC	147.87357
BB75241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.8512	WGS84	BB75241	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75241_Pcan_Bot2011_IGS	T4-11	1	BB75241_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75241	Sample	BB75241	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-11	IGS	45	BB75241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-11	CGTAATCCTCAGTACTGGCTA	147.87357
BB75241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.8512	WGS84	BB75241	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75241_Sspa_Oph2007_COI	T4-11	1	BB75241_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75241	Sample	BB75241	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-11	COI	45	BB75241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-11	GAAGGCCTTCCGATGATC	147.87357
BB75241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.8512	WGS84	BB75241	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.169	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75241_Upin_Bot2011_COI	T4-11	1	BB75241_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75241	Sample	BB75241	targeted	Present	Sample	Upin_Bot2011_COI	T4-11	COI	45	BB75241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.87357
BB75242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96131	WGS84	BB75242	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75242_Cjap_Sim2018_COI	T4-13	1	BB75242_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75242	Sample	BB75242	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-13	COI	45	BB75242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53343
BB75242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96131	WGS84	BB75242	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75242_Mgig_Bot2012_COI	T4-13	1	BB75242_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75242	Sample	BB75242	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-13	COI	45	BB75242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.53343
BB75243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96162	WGS84	BB75243	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.102	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75243_Cmae_Bot2011_COI	T4-15	1	BB75243_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75243	Sample	BB75243	targeted	Present	Sample	Cmae_Bot2011_COI	T4-15	COI	45	BB75243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-15	ATGAACAGTCTATCCTCCTTTAG	147.51952
BB75247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81044	WGS84	BB75247	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75247_Cmae_Bot2011_COI	S1-20	1	BB75247_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75247	Sample	BB75247	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-20	COI	45	BB75247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-20	ATGAACAGTCTATCCTCCTTTAG	138.52108
BB80076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.7824	WGS84	BB80076	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80076_Cmae_Bot2011_COI	S4-01	1	BB80076_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80076	Sample	BB80076	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-01	COI	45	BB80076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-01	ATGAACAGTCTATCCTCCTTTAG	138.51709
BB80078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78422	WGS84	BB80078	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80078_Aamu_Bax2006_COI	S4-03	1	BB80078_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80078	Sample	BB80078	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-03	COI	45	BB80078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-03	GCACAACCGGGATCTTTACTTC	138.51657
BB80078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78422	WGS84	BB80078	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80078_Pcan_Bot2011_IGS	S4-03	1	BB80078_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80078	Sample	BB80078	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-03	IGS	45	BB80078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-03	CGTAATCCTCAGTACTGGCTA	138.51657
BB80078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78422	WGS84	BB80078	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80078_Sspa_Oph2007_COI	S4-03	1	BB80078_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80078	Sample	BB80078	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-03	COI	45	BB80078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-03	GAAGGCCTTCCGATGATC	138.51657
BB80078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78422	WGS84	BB80078	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80078_Upin_Bot2011_COI	S4-03	1	BB80078_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80078	Sample	BB80078	targeted	Absent	Sample	Upin_Bot2011_COI	S4-03	COI	45	BB80078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.51657
BB80079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78544	WGS84	BB80079	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.414	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80079_Mgig_Bot2012_COI	S4-04	1	BB80079_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80079	Sample	BB80079	targeted	Present	Sample	Mgig_Bot2012_COI	S4-04	COI	45	BB80079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.51585
BB80081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84367	WGS84	BB80081	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80081_Aamu_Bax2006_COI	S4-06	1	BB80081_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80081	Sample	BB80081	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-06	COI	45	BB80081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-06	GCACAACCGGGATCTTTACTTC	138.49764
BB80081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84367	WGS84	BB80081	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80081_Pcan_Bot2011_IGS	S4-06	1	BB80081_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80081	Sample	BB80081	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-06	IGS	45	BB80081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-06	CGTAATCCTCAGTACTGGCTA	138.49764
BB80081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84367	WGS84	BB80081	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80081_Sspa_Oph2007_COI	S4-06	1	BB80081_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80081	Sample	BB80081	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-06	COI	45	BB80081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-06	GAAGGCCTTCCGATGATC	138.49764
BB80081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84367	WGS84	BB80081	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80081_Upin_Bot2011_COI	S4-06	1	BB80081_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80081	Sample	BB80081	targeted	Absent	Sample	Upin_Bot2011_COI	S4-06	COI	45	BB80081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.49764
BB80082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84439	WGS84	BB80082	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80082_Cjap_Sim2018_COI	S4-07	1	BB80082_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80082	Sample	BB80082	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-07	COI	45	BB80082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49743
BB80082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84439	WGS84	BB80082	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.77	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80082_Mgig_Bot2012_COI	S4-07	1	BB80082_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80082	Sample	BB80082	targeted	Present	Sample	Mgig_Bot2012_COI	S4-07	COI	45	BB80082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.49743
BB80086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84731	WGS84	BB80086	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.385	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80086_Mgig_Bot2012_COI	S4-10	1	BB80086_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80086	Sample	BB80086	targeted	Present	Sample	Mgig_Bot2012_COI	S4-10	COI	45	BB80086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.49764
BB80087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83727	WGS84	BB80087	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80087_Cmae_Bot2011_COI	S4-11	1	BB80087_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80087	Sample	BB80087	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-11	COI	45	BB80087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-11	ATGAACAGTCTATCCTCCTTTAG	138.50732
BB80089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83697	WGS84	BB80089	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80089_Aamu_Bax2006_COI	S4-13	1	BB80089_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80089	Sample	BB80089	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-13	COI	45	BB80089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-13	GCACAACCGGGATCTTTACTTC	138.50959
BB80089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83697	WGS84	BB80089	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80089_Pcan_Bot2011_IGS	S4-13	1	BB80089_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80089	Sample	BB80089	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-13	IGS	45	BB80089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-13	CGTAATCCTCAGTACTGGCTA	138.50959
BB80089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83697	WGS84	BB80089	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.492	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80089_Sspa_Oph2007_COI	S4-13	1	BB80089_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80089	Sample	BB80089	targeted	Present	Sample	Sspa_Oph2007_COI	S4-13	COI	45	BB80089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-13	GAAGGCCTTCCGATGATC	138.50959
BB80089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83697	WGS84	BB80089	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80089_Upin_Bot2011_COI	S4-13	1	BB80089_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80089	Sample	BB80089	targeted	Absent	Sample	Upin_Bot2011_COI	S4-13	COI	45	BB80089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.50959
BB80106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78368	WGS84	BB80106	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80106_Cmae_Bot2011_COI	S3-20	1	BB80106_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80106	Sample	BB80106	targeted	Absent	Sample	Cmae_Bot2011_COI	S3-20	COI	45	BB80106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-20	ATGAACAGTCTATCCTCCTTTAG	138.5164
BB80108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78601	WGS84	BB80108	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80108_Aamu_Bax2006_COI	S3-18	1	BB80108_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80108	Sample	BB80108	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-18	COI	45	BB80108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-18	GCACAACCGGGATCTTTACTTC	138.51598
BB80108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78601	WGS84	BB80108	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80108_Pcan_Bot2011_IGS	S3-18	1	BB80108_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80108	Sample	BB80108	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-18	IGS	45	BB80108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-18	CGTAATCCTCAGTACTGGCTA	138.51598
BB80108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78601	WGS84	BB80108	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80108_Sspa_Oph2007_COI	S3-18	1	BB80108_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80108	Sample	BB80108	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-18	COI	45	BB80108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-18	GAAGGCCTTCCGATGATC	138.51598
BB80108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78601	WGS84	BB80108	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80108_Upin_Bot2011_COI	S3-18	1	BB80108_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80108	Sample	BB80108	targeted	Absent	Sample	Upin_Bot2011_COI	S3-18	COI	45	BB80108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.51598
BB80109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78732	WGS84	BB80109	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.881	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80109_Mgig_Bot2012_COI	S3-17	1	BB80109_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80109	Sample	BB80109	targeted	Present	Sample	Mgig_Bot2012_COI	S3-17	COI	45	BB80109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.51553
BB80111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81136	WGS84	BB80111	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80111_Aamu_Bax2006_COI	S3-15	1	BB80111_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80111	Sample	BB80111	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-15	COI	45	BB80111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-15	GCACAACCGGGATCTTTACTTC	138.52303
BB80111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81136	WGS84	BB80111	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80111_Pcan_Bot2011_IGS	S3-15	1	BB80111_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80111	Sample	BB80111	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-15	IGS	45	BB80111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-15	CGTAATCCTCAGTACTGGCTA	138.52303
BB80111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81136	WGS84	BB80111	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80111_Sspa_Oph2007_COI	S3-15	1	BB80111_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80111	Sample	BB80111	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-15	COI	45	BB80111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-15	GAAGGCCTTCCGATGATC	138.52303
BB80111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81136	WGS84	BB80111	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80111_Upin_Bot2011_COI	S3-15	1	BB80111_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80111	Sample	BB80111	targeted	Absent	Sample	Upin_Bot2011_COI	S3-15	COI	45	BB80111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-15	CTTTAATTACAGCGTTTTTATTGTTGT	138.52303
BB80112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81082	WGS84	BB80112	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80112_Cjap_Sim2018_COI	S3-14	1	BB80112_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80112	Sample	BB80112	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-14	COI	45	BB80112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52156
BB80112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.81082	WGS84	BB80112	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.445	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80112_Mgig_Bot2012_COI	S3-14	1	BB80112_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80112	Sample	BB80112	targeted	Present	Sample	Mgig_Bot2012_COI	S3-14	COI	45	BB80112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.52156
BB80113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81015	WGS84	BB80113	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80113_Cmae_Bot2011_COI	S3-13	1	BB80113_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80113	Sample	BB80113	targeted	Absent	Sample	Cmae_Bot2011_COI	S3-13	COI	45	BB80113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-13	ATGAACAGTCTATCCTCCTTTAG	138.52006
BB80115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80903	WGS84	BB80115	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80115_Aamu_Bax2006_COI	S3-11	1	BB80115_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80115	Sample	BB80115	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-11	COI	45	BB80115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-11	GCACAACCGGGATCTTTACTTC	138.51747
BB80115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80903	WGS84	BB80115	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80115_Pcan_Bot2011_IGS	S3-11	1	BB80115_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80115	Sample	BB80115	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-11	IGS	45	BB80115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-11	CGTAATCCTCAGTACTGGCTA	138.51747
BB80115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80903	WGS84	BB80115	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80115_Sspa_Oph2007_COI	S3-11	1	BB80115_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80115	Sample	BB80115	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-11	COI	45	BB80115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-11	GAAGGCCTTCCGATGATC	138.51747
BB80115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80903	WGS84	BB80115	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80115_Upin_Bot2011_COI	S3-11	1	BB80115_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80115	Sample	BB80115	targeted	Absent	Sample	Upin_Bot2011_COI	S3-11	COI	45	BB80115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.51747
BB80116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB80116	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80116_Cjap_Sim2018_COI	S3-08	1	BB80116_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80116	Sample	BB80116	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-08	COI	45	BB80116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50858
BB80116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83674	WGS84	BB80116	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.638	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80116_Mgig_Bot2012_COI	S3-08	1	BB80116_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80116	Sample	BB80116	targeted	Present	Sample	Mgig_Bot2012_COI	S3-08	COI	45	BB80116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.50858
BB80117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83634	WGS84	BB80117	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.996	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80117_Cmae_Bot2011_COI	S3-09	1	BB80117_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80117	Sample	BB80117	targeted	Present	Sample	Cmae_Bot2011_COI	S3-09	COI	45	BB80117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-09	ATGAACAGTCTATCCTCCTTTAG	138.5092
BB80119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83679	WGS84	BB80119	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80119_Aamu_Bax2006_COI	S3-07	1	BB80119_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80119	Sample	BB80119	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-07	COI	45	BB80119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-07	GCACAACCGGGATCTTTACTTC	138.50733
BB80119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83679	WGS84	BB80119	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80119_Pcan_Bot2011_IGS	S3-07	1	BB80119_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80119	Sample	BB80119	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-07	IGS	45	BB80119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-07	CGTAATCCTCAGTACTGGCTA	138.50733
BB80119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83679	WGS84	BB80119	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.996	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80119_Sspa_Oph2007_COI	S3-07	1	BB80119_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80119	Sample	BB80119	targeted	Present	Sample	Sspa_Oph2007_COI	S3-07	COI	45	BB80119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-07	GAAGGCCTTCCGATGATC	138.50733
BB80119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83679	WGS84	BB80119	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80119_Upin_Bot2011_COI	S3-07	1	BB80119_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80119	Sample	BB80119	targeted	Absent	Sample	Upin_Bot2011_COI	S3-07	COI	45	BB80119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.50733
BB80120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83819	WGS84	BB80120	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80120_Cmae_Bot2011_COI	S3-06	1	BB80120_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80120	Sample	BB80120	targeted	Absent	Sample	Cmae_Bot2011_COI	S3-06	COI	45	BB80120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-06	ATGAACAGTCTATCCTCCTTTAG	138.50691
BB80122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84355	WGS84	BB80122	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80122_Aamu_Bax2006_COI	S3-04	1	BB80122_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80122	Sample	BB80122	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-04	COI	45	BB80122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-04	GCACAACCGGGATCTTTACTTC	138.49824
BB80122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84355	WGS84	BB80122	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80122_Pcan_Bot2011_IGS	S3-04	1	BB80122_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80122	Sample	BB80122	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-04	IGS	45	BB80122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-04	CGTAATCCTCAGTACTGGCTA	138.49824
BB80122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84355	WGS84	BB80122	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80122_Sspa_Oph2007_COI	S3-04	1	BB80122_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80122	Sample	BB80122	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-04	COI	45	BB80122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-04	GAAGGCCTTCCGATGATC	138.49824
BB80122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84355	WGS84	BB80122	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80122_Upin_Bot2011_COI	S3-04	1	BB80122_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80122	Sample	BB80122	targeted	Absent	Sample	Upin_Bot2011_COI	S3-04	COI	45	BB80122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.49824
BB80123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84423	WGS84	BB80123	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80123_Cjap_Sim2018_COI	S3-03	1	BB80123_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80123	Sample	BB80123	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-03	COI	45	BB80123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49776
BB80123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84423	WGS84	BB80123	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.482	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80123_Mgig_Bot2012_COI	S3-03	1	BB80123_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80123	Sample	BB80123	targeted	Present	Sample	Mgig_Bot2012_COI	S3-03	COI	45	BB80123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.49776
BB80124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8452	WGS84	BB80124	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.127	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80124_Cmae_Bot2011_COI	S3-02	1	BB80124_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80124	Sample	BB80124	targeted	Present	Sample	Cmae_Bot2011_COI	S3-02	COI	45	BB80124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-02	ATGAACAGTCTATCCTCCTTTAG	138.4972
BB80145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38774	WGS84	BB80145	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80145_Mgig_Bot2012_COI	BB22	1	BB80145_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80145	Sample	BB80145	targeted	Absent	Sample	Mgig_Bot2012_COI	BB22	COI	45	BB80145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB22	TCTTATTCGTTGGAGACTTTATAACCCT	153.15869
BB80146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35841	WGS84	BB80146	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80146_Cmae_Bot2011_COI	BB2	1	BB80146_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80146	Sample	BB80146	targeted	Absent	Sample	Cmae_Bot2011_COI	BB2	COI	45	BB80146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB2	ATGAACAGTCTATCCTCCTTTAG	153.17941
BB80146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35841	WGS84	BB80146	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80146_Msal_Bot2012_COI	BB2	1	BB80146_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80146	Sample	BB80146	targeted	Absent	Sample	Msal_Bot2012_COI	BB2	COI	45	BB80146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB2	GYTAGTTCCRATGATGTTAGCTG	153.17941
BB80148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38	WGS84	BB80148	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80148_Aamu_Bax2006_COI	BB16	1	BB80148_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80148	Sample	BB80148	targeted	Absent	Sample	Aamu_Bax2006_COI	BB16	COI	45	BB80148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB16	GCACAACCGGGATCTTTACTTC	153.16327
BB80148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38	WGS84	BB80148	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80148_Pcan_Bot2011_IGS	BB16	1	BB80148_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80148	Sample	BB80148	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB16	IGS	45	BB80148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB16	CGTAATCCTCAGTACTGGCTA	153.16327
BB80148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38	WGS84	BB80148	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80148_Sspa_Oph2007_COI	BB16	1	BB80148_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80148	Sample	BB80148	targeted	Absent	Sample	Sspa_Oph2007_COI	BB16	COI	45	BB80148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB16	GAAGGCCTTCCGATGATC	153.16327
BB80148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38	WGS84	BB80148	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80148_Upin_Bot2011_COI	BB16	1	BB80148_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80148	Sample	BB80148	targeted	Absent	Sample	Upin_Bot2011_COI	BB16	COI	45	BB80148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB16	CTTTAATTACAGCGTTTTTATTGTTGT	153.16327
BB80149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.376	WGS84	BB80149	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80149_Mgig_Bot2012_COI	BB13	1	BB80149_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80149	Sample	BB80149	targeted	Absent	Sample	Mgig_Bot2012_COI	BB13	COI	45	BB80149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB13	TCTTATTCGTTGGAGACTTTATAACCCT	153.16704
BB80151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35698	WGS84	BB80151	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80151_Aamu_Bax2006_COI	BB1	1	BB80151_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80151	Sample	BB80151	targeted	Absent	Sample	Aamu_Bax2006_COI	BB1	COI	45	BB80151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB1	GCACAACCGGGATCTTTACTTC	153.18056
BB80151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35698	WGS84	BB80151	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80151_Pcan_Bot2011_IGS	BB1	1	BB80151_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80151	Sample	BB80151	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB1	IGS	45	BB80151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB1	CGTAATCCTCAGTACTGGCTA	153.18056
BB80151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35698	WGS84	BB80151	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80151_Sspa_Oph2007_COI	BB1	1	BB80151_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80151	Sample	BB80151	targeted	Absent	Sample	Sspa_Oph2007_COI	BB1	COI	45	BB80151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB1	GAAGGCCTTCCGATGATC	153.18056
BB80151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35698	WGS84	BB80151	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80151_Upin_Bot2011_COI	BB1	1	BB80151_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80151	Sample	BB80151	targeted	Absent	Sample	Upin_Bot2011_COI	BB1	COI	45	BB80151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB1	CTTTAATTACAGCGTTTTTATTGTTGT	153.18056
BB80152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44756	WGS84	BB80152	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80152_Mgig_Bot2012_COI	BB64	1	BB80152_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80152	Sample	BB80152	targeted	Absent	Sample	Mgig_Bot2012_COI	BB64	COI	45	BB80152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB64	TCTTATTCGTTGGAGACTTTATAACCCT	153.09609
BB80153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44718	WGS84	BB80153	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80153_Cmae_Bot2011_COI	BB63	1	BB80153_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80153	Sample	BB80153	targeted	Absent	Sample	Cmae_Bot2011_COI	BB63	COI	45	BB80153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB63	ATGAACAGTCTATCCTCCTTTAG	153.09844
BB80153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44718	WGS84	BB80153	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80153_Msal_Bot2012_COI	BB63	1	BB80153_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80153	Sample	BB80153	targeted	Absent	Sample	Msal_Bot2012_COI	BB63	COI	45	BB80153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB63	GYTAGTTCCRATGATGTTAGCTG	153.09844
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80155_Aamu_Bax2006_COI	MB85	1	BB80155_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Absent	Sample	Aamu_Bax2006_COI	MB85	COI	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	GCACAACCGGGATCTTTACTTC	144.92112
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80155_Pcan_Bot2011_IGS	MB85	1	BB80155_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB85	IGS	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	CGTAATCCTCAGTACTGGCTA	144.92112
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.95352173	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80155_Sspa_Oph2007_COI	MB85	1	BB80155_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Present	Sample	Sspa_Oph2007_COI	MB85	COI	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	GAAGGCCTTCCGATGATC	144.92112
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80155_Upin_Bot2011_COI	MB85	1	BB80155_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Absent	Sample	Upin_Bot2011_COI	MB85	COI	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	CTTTAATTACAGCGTTTTTATTGTTGT	144.92112
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80156_Mgig_Bot2012_COI	MB80	1	BB80156_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Absent	Sample	Mgig_Bot2012_COI	MB80	COI	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	TCTTATTCGTTGGAGACTTTATAACCCT	144.92768
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80157_Cmae_Bot2011_COI	MB65	1	BB80157_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Cmae_Bot2011_COI	MB65	COI	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	ATGAACAGTCTATCCTCCTTTAG	144.922
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80157_Hsan_Wil2021_COI	MB65	1	BB80157_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Hsan_Wil2021_COI	MB65	COI	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	CCTGGGCCGGTATAGTAGGT	144.922
BB80157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81992	WGS84	BB80157	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80157_Msal_Bot2012_COI	MB65	1	BB80157_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80157	Sample	BB80157	targeted	Absent	Sample	Msal_Bot2012_COI	MB65	COI	45	BB80157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB65	GYTAGTTCCRATGATGTTAGCTG	144.922
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80159_Aamu_Bax2006_COI	MB105	1	BB80159_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Absent	Sample	Aamu_Bax2006_COI	MB105	COI	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	GCACAACCGGGATCTTTACTTC	144.91163
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80159_Pcan_Bot2011_IGS	MB105	1	BB80159_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB105	IGS	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	CGTAATCCTCAGTACTGGCTA	144.91163
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.37466812	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80159_Sspa_Oph2007_COI	MB105	1	BB80159_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Present	Sample	Sspa_Oph2007_COI	MB105	COI	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	GAAGGCCTTCCGATGATC	144.91163
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	43.409	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80159_Upin_Bot2011_COI	MB105	1	BB80159_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Present	Sample	Upin_Bot2011_COI	MB105	COI	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	CTTTAATTACAGCGTTTTTATTGTTGT	144.91163
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80160_Cmae_Bot2011_COI	MB100	1	BB80160_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Absent	Sample	Cmae_Bot2011_COI	MB100	COI	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	ATGAACAGTCTATCCTCCTTTAG	144.91317
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80160_Hsan_Wil2021_COI	MB100	1	BB80160_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Absent	Sample	Hsan_Wil2021_COI	MB100	COI	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	CCTGGGCCGGTATAGTAGGT	144.91317
BB80160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81133	WGS84	BB80160	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80160_Msal_Bot2012_COI	MB100	1	BB80160_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80160	Sample	BB80160	targeted	Absent	Sample	Msal_Bot2012_COI	MB100	COI	45	BB80160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB100	GYTAGTTCCRATGATGTTAGCTG	144.91317
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80162_Aamu_Bax2006_COI	MB110	1	BB80162_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Absent	Sample	Aamu_Bax2006_COI	MB110	COI	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	GCACAACCGGGATCTTTACTTC	144.90763
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80162_Pcan_Bot2011_IGS	MB110	1	BB80162_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB110	IGS	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	CGTAATCCTCAGTACTGGCTA	144.90763
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.53149414	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80162_Sspa_Oph2007_COI	MB110	1	BB80162_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Present	Sample	Sspa_Oph2007_COI	MB110	COI	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	GAAGGCCTTCCGATGATC	144.90763
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80162_Upin_Bot2011_COI	MB110	1	BB80162_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Absent	Sample	Upin_Bot2011_COI	MB110	COI	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	CTTTAATTACAGCGTTTTTATTGTTGT	144.90763
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80163_Mgig_Bot2012_COI	MB50	1	BB80163_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Absent	Sample	Mgig_Bot2012_COI	MB50	COI	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	TCTTATTCGTTGGAGACTTTATAACCCT	144.90737
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80164_Cmae_Bot2011_COI	MB45	1	BB80164_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Absent	Sample	Cmae_Bot2011_COI	MB45	COI	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	ATGAACAGTCTATCCTCCTTTAG	144.91008
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80164_Hsan_Wil2021_COI	MB45	1	BB80164_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Absent	Sample	Hsan_Wil2021_COI	MB45	COI	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	CCTGGGCCGGTATAGTAGGT	144.91008
BB80164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86129	WGS84	BB80164	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80164_Msal_Bot2012_COI	MB45	1	BB80164_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80164	Sample	BB80164	targeted	Absent	Sample	Msal_Bot2012_COI	MB45	COI	45	BB80164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB45	GYTAGTTCCRATGATGTTAGCTG	144.91008
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.685	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80166_Aamu_Bax2006_COI	MB35	1	BB80166_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Present	Sample	Aamu_Bax2006_COI	MB35	COI	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	GCACAACCGGGATCTTTACTTC	144.91773
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80166_Pcan_Bot2011_IGS	MB35	1	BB80166_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB35	IGS	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	CGTAATCCTCAGTACTGGCTA	144.91773
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.60627365	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80166_Sspa_Oph2007_COI	MB35	1	BB80166_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Present	Sample	Sspa_Oph2007_COI	MB35	COI	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	GAAGGCCTTCCGATGATC	144.91773
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.076	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80166_Upin_Bot2011_COI	MB35	1	BB80166_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Present	Sample	Upin_Bot2011_COI	MB35	COI	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	CTTTAATTACAGCGTTTTTATTGTTGT	144.91773
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80167_Mgig_Bot2012_COI	MB30	1	BB80167_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Absent	Sample	Mgig_Bot2012_COI	MB30	COI	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	TCTTATTCGTTGGAGACTTTATAACCCT	144.91604
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80168_Cmae_Bot2011_COI	MB5	1	BB80168_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Absent	Sample	Cmae_Bot2011_COI	MB5	COI	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	ATGAACAGTCTATCCTCCTTTAG	144.9048
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80168_Hsan_Wil2021_COI	MB5	1	BB80168_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Absent	Sample	Hsan_Wil2021_COI	MB5	COI	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	CCTGGGCCGGTATAGTAGGT	144.9048
BB80168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84114	WGS84	BB80168	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80168_Msal_Bot2012_COI	MB5	1	BB80168_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80168	Sample	BB80168	targeted	Absent	Sample	Msal_Bot2012_COI	MB5	COI	45	BB80168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB5	GYTAGTTCCRATGATGTTAGCTG	144.9048
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80170_Mgig_Bot2012_COI	MB10	1	BB80170_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Absent	Sample	Mgig_Bot2012_COI	MB10	COI	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	TCTTATTCGTTGGAGACTTTATAACCCT	144.90737
BB80171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84287	WGS84	BB80171	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80171_Cmae_Bot2011_COI	MB20	1	BB80171_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80171	Sample	BB80171	targeted	Absent	Sample	Cmae_Bot2011_COI	MB20	COI	45	BB80171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB20	ATGAACAGTCTATCCTCCTTTAG	144.92678
BB80171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84287	WGS84	BB80171	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80171_Msal_Bot2012_COI	MB20	1	BB80171_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80171	Sample	BB80171	targeted	Absent	Sample	Msal_Bot2012_COI	MB20	COI	45	BB80171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB20	GYTAGTTCCRATGATGTTAGCTG	144.92678
BB80177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81974	WGS84	BB80177	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80177_Aamu_Bax2006_COI	MB108	1	BB80177_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80177	Sample	BB80177	targeted	Absent	Sample	Aamu_Bax2006_COI	MB108	COI	45	BB80177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB108	GCACAACCGGGATCTTTACTTC	144.90959
BB80177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81974	WGS84	BB80177	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80177_Pcan_Bot2011_IGS	MB108	1	BB80177_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80177	Sample	BB80177	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB108	IGS	45	BB80177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB108	CGTAATCCTCAGTACTGGCTA	144.90959
BB80177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81974	WGS84	BB80177	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.15131378	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80177_Sspa_Oph2007_COI	MB108	1	BB80177_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80177	Sample	BB80177	targeted	Present	Sample	Sspa_Oph2007_COI	MB108	COI	45	BB80177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB108	GAAGGCCTTCCGATGATC	144.90959
BB80177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81974	WGS84	BB80177	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	41.338	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80177_Upin_Bot2011_COI	MB108	1	BB80177_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80177	Sample	BB80177	targeted	Present	Sample	Upin_Bot2011_COI	MB108	COI	45	BB80177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB108	CTTTAATTACAGCGTTTTTATTGTTGT	144.90959
BB80178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81639	WGS84	BB80178	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.757	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80178_Mgig_Bot2012_COI	MB104	1	BB80178_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80178	Sample	BB80178	targeted	Present	Sample	Mgig_Bot2012_COI	MB104	COI	45	BB80178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB104	TCTTATTCGTTGGAGACTTTATAACCCT	144.91286
BB80179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81378	WGS84	BB80179	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80179_Cmae_Bot2011_COI	MB102	1	BB80179_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80179	Sample	BB80179	targeted	Absent	Sample	Cmae_Bot2011_COI	MB102	COI	45	BB80179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB102	ATGAACAGTCTATCCTCCTTTAG	144.91311
BB80179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81378	WGS84	BB80179	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80179_Msal_Bot2012_COI	MB102	1	BB80179_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80179	Sample	BB80179	targeted	Absent	Sample	Msal_Bot2012_COI	MB102	COI	45	BB80179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB102	GYTAGTTCCRATGATGTTAGCTG	144.91311
BB80180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81	WGS84	BB80180	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80180_Aamu_Bax2006_COI	MB98	1	BB80180_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80180	Sample	BB80180	targeted	Absent	Sample	Aamu_Bax2006_COI	MB98	COI	45	BB80180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB98	GCACAACCGGGATCTTTACTTC	144.91304
BB80180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81	WGS84	BB80180	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80180_Pcan_Bot2011_IGS	MB98	1	BB80180_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80180	Sample	BB80180	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB98	IGS	45	BB80180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB98	CGTAATCCTCAGTACTGGCTA	144.91304
BB80180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81	WGS84	BB80180	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.78356934	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80180_Sspa_Oph2007_COI	MB98	1	BB80180_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80180	Sample	BB80180	targeted	Present	Sample	Sspa_Oph2007_COI	MB98	COI	45	BB80180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB98	GAAGGCCTTCCGATGATC	144.91304
BB80180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81	WGS84	BB80180	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80180_Upin_Bot2011_COI	MB98	1	BB80180_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80180	Sample	BB80180	targeted	Absent	Sample	Upin_Bot2011_COI	MB98	COI	45	BB80180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB98	CTTTAATTACAGCGTTTTTATTGTTGT	144.91304
BB80181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.80956	WGS84	BB80181	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.497	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80181_Mgig_Bot2012_COI	MB96	1	BB80181_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80181	Sample	BB80181	targeted	Present	Sample	Mgig_Bot2012_COI	MB96	COI	45	BB80181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB96	TCTTATTCGTTGGAGACTTTATAACCCT	144.91452
BB80182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81211	WGS84	BB80182	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80182_Cmae_Bot2011_COI	MB94	1	BB80182_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80182	Sample	BB80182	targeted	Absent	Sample	Cmae_Bot2011_COI	MB94	COI	45	BB80182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB94	ATGAACAGTCTATCCTCCTTTAG	144.91485
BB80182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81211	WGS84	BB80182	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80182_Msal_Bot2012_COI	MB94	1	BB80182_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80182	Sample	BB80182	targeted	Absent	Sample	Msal_Bot2012_COI	MB94	COI	45	BB80182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB94	GYTAGTTCCRATGATGTTAGCTG	144.91485
BB80184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81828	WGS84	BB80184	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.463	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80184_Aamu_Bax2006_COI	MB89	1	BB80184_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80184	Sample	BB80184	targeted	Present	Sample	Aamu_Bax2006_COI	MB89	COI	45	BB80184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB89	GCACAACCGGGATCTTTACTTC	144.91499
BB80184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81828	WGS84	BB80184	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80184_Pcan_Bot2011_IGS	MB89	1	BB80184_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80184	Sample	BB80184	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB89	IGS	45	BB80184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB89	CGTAATCCTCAGTACTGGCTA	144.91499
BB80184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81828	WGS84	BB80184	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.20515823	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80184_Sspa_Oph2007_COI	MB89	1	BB80184_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80184	Sample	BB80184	targeted	Present	Sample	Sspa_Oph2007_COI	MB89	COI	45	BB80184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB89	GAAGGCCTTCCGATGATC	144.91499
BB80184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81828	WGS84	BB80184	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80184_Upin_Bot2011_COI	MB89	1	BB80184_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80184	Sample	BB80184	targeted	Absent	Sample	Upin_Bot2011_COI	MB89	COI	45	BB80184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB89	CTTTAATTACAGCGTTTTTATTGTTGT	144.91499
BB80185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81841	WGS84	BB80185	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80185_Mgig_Bot2012_COI	MB87	1	BB80185_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80185	Sample	BB80185	targeted	Absent	Sample	Mgig_Bot2012_COI	MB87	COI	45	BB80185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB87	TCTTATTCGTTGGAGACTTTATAACCCT	144.91788
BB80186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81617	WGS84	BB80186	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80186_Cmae_Bot2011_COI	MB83	1	BB80186_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80186	Sample	BB80186	targeted	Absent	Sample	Cmae_Bot2011_COI	MB83	COI	45	BB80186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB83	ATGAACAGTCTATCCTCCTTTAG	144.92378
BB80186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81617	WGS84	BB80186	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80186_Msal_Bot2012_COI	MB83	1	BB80186_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80186	Sample	BB80186	targeted	Absent	Sample	Msal_Bot2012_COI	MB83	COI	45	BB80186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB83	GYTAGTTCCRATGATGTTAGCTG	144.92378
BB80188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81667	WGS84	BB80188	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80188_Aamu_Bax2006_COI	MB78	1	BB80188_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80188	Sample	BB80188	targeted	Absent	Sample	Aamu_Bax2006_COI	MB78	COI	45	BB80188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB78	GCACAACCGGGATCTTTACTTC	144.92656
BB80188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81667	WGS84	BB80188	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80188_Pcan_Bot2011_IGS	MB78	1	BB80188_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80188	Sample	BB80188	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB78	IGS	45	BB80188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB78	CGTAATCCTCAGTACTGGCTA	144.92656
BB80188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81667	WGS84	BB80188	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80188_Sspa_Oph2007_COI	MB78	1	BB80188_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80188	Sample	BB80188	targeted	Absent	Sample	Sspa_Oph2007_COI	MB78	COI	45	BB80188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB78	GAAGGCCTTCCGATGATC	144.92656
BB80188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81667	WGS84	BB80188	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80188_Upin_Bot2011_COI	MB78	1	BB80188_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80188	Sample	BB80188	targeted	Absent	Sample	Upin_Bot2011_COI	MB78	COI	45	BB80188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB78	CTTTAATTACAGCGTTTTTATTGTTGT	144.92656
BB80189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81818	WGS84	BB80189	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80189_Mgig_Bot2012_COI	MB76	1	BB80189_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80189	Sample	BB80189	targeted	Absent	Sample	Mgig_Bot2012_COI	MB76	COI	45	BB80189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB76	TCTTATTCGTTGGAGACTTTATAACCCT	144.92699
BB80191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81807	WGS84	BB80191	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80191_Aamu_Bax2006_COI	MB72	1	BB80191_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80191	Sample	BB80191	targeted	Absent	Sample	Aamu_Bax2006_COI	MB72	COI	45	BB80191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB72	GCACAACCGGGATCTTTACTTC	144.93189
BB80191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81807	WGS84	BB80191	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80191_Pcan_Bot2011_IGS	MB72	1	BB80191_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80191	Sample	BB80191	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB72	IGS	45	BB80191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB72	CGTAATCCTCAGTACTGGCTA	144.93189
BB80191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81807	WGS84	BB80191	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80191_Sspa_Oph2007_COI	MB72	1	BB80191_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80191	Sample	BB80191	targeted	Absent	Sample	Sspa_Oph2007_COI	MB72	COI	45	BB80191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB72	GAAGGCCTTCCGATGATC	144.93189
BB80191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81807	WGS84	BB80191	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80191_Upin_Bot2011_COI	MB72	1	BB80191_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80191	Sample	BB80191	targeted	Absent	Sample	Upin_Bot2011_COI	MB72	COI	45	BB80191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB72	CTTTAATTACAGCGTTTTTATTGTTGT	144.93189
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81889	WGS84	BB80192	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	38.033	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80192_Asen_Wil2025_COI	MB71	1	BB80192_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Present	Sample	Asen_Wil2025_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	GTTGTTGTTACAACACATGCTCTTTTG	144.93166
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81889	WGS84	BB80192	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.92	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80192_Mgig_Bot2012_COI	MB71	1	BB80192_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Present	Sample	Mgig_Bot2012_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	TCTTATTCGTTGGAGACTTTATAACCCT	144.93166
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82058	WGS84	BB80193	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80193_Cmae_Bot2011_COI	MB69	1	BB80193_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Absent	Sample	Cmae_Bot2011_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	ATGAACAGTCTATCCTCCTTTAG	144.93081
BB80193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82058	WGS84	BB80193	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80193_Msal_Bot2012_COI	MB69	1	BB80193_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80193	Sample	BB80193	targeted	Absent	Sample	Msal_Bot2012_COI	MB69	COI	45	BB80193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB69	GYTAGTTCCRATGATGTTAGCTG	144.93081
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81776	WGS84	BB80195	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80195_Aamu_Bax2006_COI	MB77	1	BB80195_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Absent	Sample	Aamu_Bax2006_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	GCACAACCGGGATCTTTACTTC	144.92569
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81776	WGS84	BB80195	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80195_Pcan_Bot2011_IGS	MB77	1	BB80195_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB77	IGS	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	CGTAATCCTCAGTACTGGCTA	144.92569
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81776	WGS84	BB80195	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.83216476	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80195_Sspa_Oph2007_COI	MB77	1	BB80195_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Present	Sample	Sspa_Oph2007_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	GAAGGCCTTCCGATGATC	144.92569
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81776	WGS84	BB80195	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80195_Upin_Bot2011_COI	MB77	1	BB80195_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Absent	Sample	Upin_Bot2011_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	CTTTAATTACAGCGTTTTTATTGTTGT	144.92569
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81776	WGS84	BB80195	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80195_Vgib_Wil2025_COI	MB77	1	BB80195_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Absent	Sample	Vgib_Wil2025_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	CCCCTCTTTACAAGTTCTTTAGGGT	144.92569
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81986	WGS84	BB80196	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.661	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80196_Asen_Wil2025_COI	MB66	1	BB80196_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Present	Sample	Asen_Wil2025_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	GTTGTTGTTACAACACATGCTCTTTTG	144.9239
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81986	WGS84	BB80196	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80196_Mgig_Bot2012_COI	MB66	1	BB80196_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Mgig_Bot2012_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	TCTTATTCGTTGGAGACTTTATAACCCT	144.9239
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82016	WGS84	BB80197	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80197_Cmae_Bot2011_COI	MB63	1	BB80197_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Absent	Sample	Cmae_Bot2011_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	ATGAACAGTCTATCCTCCTTTAG	144.91788
BB80197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82016	WGS84	BB80197	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80197_Msal_Bot2012_COI	MB63	1	BB80197_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80197	Sample	BB80197	targeted	Absent	Sample	Msal_Bot2012_COI	MB63	COI	45	BB80197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB63	GYTAGTTCCRATGATGTTAGCTG	144.91788
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82144	WGS84	BB80199	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80199_Aamu_Bax2006_COI	MB61	1	BB80199_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Absent	Sample	Aamu_Bax2006_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	GCACAACCGGGATCTTTACTTC	144.91152
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82144	WGS84	BB80199	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80199_Pcan_Bot2011_IGS	MB61	1	BB80199_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB61	IGS	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	CGTAATCCTCAGTACTGGCTA	144.91152
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82144	WGS84	BB80199	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.75011826	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80199_Sspa_Oph2007_COI	MB61	1	BB80199_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Present	Sample	Sspa_Oph2007_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	GAAGGCCTTCCGATGATC	144.91152
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82144	WGS84	BB80199	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80199_Upin_Bot2011_COI	MB61	1	BB80199_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Absent	Sample	Upin_Bot2011_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	CTTTAATTACAGCGTTTTTATTGTTGT	144.91152
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82144	WGS84	BB80199	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80199_Vgib_Wil2025_COI	MB61	1	BB80199_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Absent	Sample	Vgib_Wil2025_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	CCCCTCTTTACAAGTTCTTTAGGGT	144.91152
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82492	WGS84	BB80200	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	35.678	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80200_Asen_Wil2025_COI	MB58	1	BB80200_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Present	Sample	Asen_Wil2025_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	GTTGTTGTTACAACACATGCTCTTTTG	144.90413
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82492	WGS84	BB80200	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80200_Mgig_Bot2012_COI	MB58	1	BB80200_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Absent	Sample	Mgig_Bot2012_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	TCTTATTCGTTGGAGACTTTATAACCCT	144.90413
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82853	WGS84	BB80201	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80201_Cmae_Bot2011_COI	MB56	1	BB80201_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Absent	Sample	Cmae_Bot2011_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	ATGAACAGTCTATCCTCCTTTAG	144.90063
BB80201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82853	WGS84	BB80201	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80201_Msal_Bot2012_COI	MB56	1	BB80201_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80201	Sample	BB80201	targeted	Absent	Sample	Msal_Bot2012_COI	MB56	COI	45	BB80201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB56	GYTAGTTCCRATGATGTTAGCTG	144.90063
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.825	WGS84	BB80203	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80203_Aamu_Bax2006_COI	MB124	1	BB80203_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Absent	Sample	Aamu_Bax2006_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	GCACAACCGGGATCTTTACTTC	144.90061
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.825	WGS84	BB80203	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80203_Pcan_Bot2011_IGS	MB124	1	BB80203_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB124	IGS	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	CGTAATCCTCAGTACTGGCTA	144.90061
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.825	WGS84	BB80203	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.79381561	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80203_Sspa_Oph2007_COI	MB124	1	BB80203_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Present	Sample	Sspa_Oph2007_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	GAAGGCCTTCCGATGATC	144.90061
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.825	WGS84	BB80203	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80203_Upin_Bot2011_COI	MB124	1	BB80203_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Absent	Sample	Upin_Bot2011_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	CTTTAATTACAGCGTTTTTATTGTTGT	144.90061
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.825	WGS84	BB80203	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80203_Vgib_Wil2025_COI	MB124	1	BB80203_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Absent	Sample	Vgib_Wil2025_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	CCCCTCTTTACAAGTTCTTTAGGGT	144.90061
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82329	WGS84	BB80204	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.065	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80204_Asen_Wil2025_COI	MB121	1	BB80204_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Present	Sample	Asen_Wil2025_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	GTTGTTGTTACAACACATGCTCTTTTG	144.90234
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82329	WGS84	BB80204	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.721	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80204_Mgig_Bot2012_COI	MB121	1	BB80204_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Present	Sample	Mgig_Bot2012_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	TCTTATTCGTTGGAGACTTTATAACCCT	144.90234
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82145	WGS84	BB80205	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80205_Cmae_Bot2011_COI	MB119	1	BB80205_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Absent	Sample	Cmae_Bot2011_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	ATGAACAGTCTATCCTCCTTTAG	144.90501
BB80205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82145	WGS84	BB80205	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80205_Msal_Bot2012_COI	MB119	1	BB80205_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80205	Sample	BB80205	targeted	Absent	Sample	Msal_Bot2012_COI	MB119	COI	45	BB80205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB119	GYTAGTTCCRATGATGTTAGCTG	144.90501
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81725	WGS84	BB80207	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.182	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80207_Aamu_Bax2006_COI	MB113	1	BB80207_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Present	Sample	Aamu_Bax2006_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	GCACAACCGGGATCTTTACTTC	144.90634
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81725	WGS84	BB80207	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80207_Pcan_Bot2011_IGS	MB113	1	BB80207_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB113	IGS	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	CGTAATCCTCAGTACTGGCTA	144.90634
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81725	WGS84	BB80207	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.44155502	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80207_Sspa_Oph2007_COI	MB113	1	BB80207_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Present	Sample	Sspa_Oph2007_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	GAAGGCCTTCCGATGATC	144.90634
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81725	WGS84	BB80207	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80207_Upin_Bot2011_COI	MB113	1	BB80207_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Absent	Sample	Upin_Bot2011_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	CTTTAATTACAGCGTTTTTATTGTTGT	144.90634
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81725	WGS84	BB80207	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	35.812	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80207_Vgib_Wil2025_COI	MB113	1	BB80207_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Present	Sample	Vgib_Wil2025_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	CCCCTCTTTACAAGTTCTTTAGGGT	144.90634
BB80208	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81853	WGS84	BB80208	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.718	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80208_Mgig_Bot2012_COI	MB112	1	BB80208_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80208	Sample	BB80208	targeted	Present	Sample	Mgig_Bot2012_COI	MB112	COI	45	BB80208	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB112	TCTTATTCGTTGGAGACTTTATAACCCT	144.90678
BB80209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85495	WGS84	BB80209	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80209_Cmae_Bot2011_COI	MB54	1	BB80209_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80209	Sample	BB80209	targeted	Absent	Sample	Cmae_Bot2011_COI	MB54	COI	45	BB80209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB54	ATGAACAGTCTATCCTCCTTTAG	144.90386
BB80209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85495	WGS84	BB80209	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80209_Msal_Bot2012_COI	MB54	1	BB80209_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80209	Sample	BB80209	targeted	Absent	Sample	Msal_Bot2012_COI	MB54	COI	45	BB80209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB54	GYTAGTTCCRATGATGTTAGCTG	144.90386
BB80210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85743	WGS84	BB80210	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.91	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80210_Aamu_Bax2006_COI	MB52	1	BB80210_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80210	Sample	BB80210	targeted	Present	Sample	Aamu_Bax2006_COI	MB52	COI	45	BB80210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB52	GCACAACCGGGATCTTTACTTC	144.90744
BB80210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85743	WGS84	BB80210	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80210_Pcan_Bot2011_IGS	MB52	1	BB80210_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80210	Sample	BB80210	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB52	IGS	45	BB80210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB52	CGTAATCCTCAGTACTGGCTA	144.90744
BB80210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85743	WGS84	BB80210	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.79454422	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80210_Sspa_Oph2007_COI	MB52	1	BB80210_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80210	Sample	BB80210	targeted	Present	Sample	Sspa_Oph2007_COI	MB52	COI	45	BB80210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB52	GAAGGCCTTCCGATGATC	144.90744
BB80210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85743	WGS84	BB80210	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.613	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80210_Upin_Bot2011_COI	MB52	1	BB80210_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80210	Sample	BB80210	targeted	Present	Sample	Upin_Bot2011_COI	MB52	COI	45	BB80210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB52	CTTTAATTACAGCGTTTTTATTGTTGT	144.90744
BB80211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.861	WGS84	BB80211	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.837	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80211_Mgig_Bot2012_COI	MB49	1	BB80211_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80211	Sample	BB80211	targeted	Present	Sample	Mgig_Bot2012_COI	MB49	COI	45	BB80211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB49	TCTTATTCGTTGGAGACTTTATAACCCT	144.90817
BB80212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86015	WGS84	BB80212	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80212_Cmae_Bot2011_COI	MB47	1	BB80212_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80212	Sample	BB80212	targeted	Absent	Sample	Cmae_Bot2011_COI	MB47	COI	45	BB80212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB47	ATGAACAGTCTATCCTCCTTTAG	144.90951
BB80212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86015	WGS84	BB80212	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80212_Msal_Bot2012_COI	MB47	1	BB80212_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80212	Sample	BB80212	targeted	Absent	Sample	Msal_Bot2012_COI	MB47	COI	45	BB80212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB47	GYTAGTTCCRATGATGTTAGCTG	144.90951
BB80214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86081	WGS84	BB80214	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.693	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80214_Aamu_Bax2006_COI	MB43	1	BB80214_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80214	Sample	BB80214	targeted	Present	Sample	Aamu_Bax2006_COI	MB43	COI	45	BB80214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB43	GCACAACCGGGATCTTTACTTC	144.91408
BB80214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86081	WGS84	BB80214	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80214_Pcan_Bot2011_IGS	MB43	1	BB80214_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80214	Sample	BB80214	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB43	IGS	45	BB80214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB43	CGTAATCCTCAGTACTGGCTA	144.91408
BB80214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86081	WGS84	BB80214	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.04564285	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80214_Sspa_Oph2007_COI	MB43	1	BB80214_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80214	Sample	BB80214	targeted	Present	Sample	Sspa_Oph2007_COI	MB43	COI	45	BB80214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB43	GAAGGCCTTCCGATGATC	144.91408
BB80214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86081	WGS84	BB80214	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.791	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80214_Upin_Bot2011_COI	MB43	1	BB80214_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80214	Sample	BB80214	targeted	Present	Sample	Upin_Bot2011_COI	MB43	COI	45	BB80214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB43	CTTTAATTACAGCGTTTTTATTGTTGT	144.91408
BB80215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86302	WGS84	BB80215	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80215_Mgig_Bot2012_COI	MB39	1	BB80215_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80215	Sample	BB80215	targeted	Absent	Sample	Mgig_Bot2012_COI	MB39	COI	45	BB80215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB39	TCTTATTCGTTGGAGACTTTATAACCCT	144.9144
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80216_Cmae_Bot2011_COI	MB38	1	BB80216_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Absent	Sample	Cmae_Bot2011_COI	MB38	COI	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	ATGAACAGTCTATCCTCCTTTAG	144.91498
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80216_Hsan_Wil2021_COI	MB38	1	BB80216_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Absent	Sample	Hsan_Wil2021_COI	MB38	COI	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	CCTGGGCCGGTATAGTAGGT	144.91498
BB80216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8622	WGS84	BB80216	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80216_Msal_Bot2012_COI	MB38	1	BB80216_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80216	Sample	BB80216	targeted	Absent	Sample	Msal_Bot2012_COI	MB38	COI	45	BB80216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB38	GYTAGTTCCRATGATGTTAGCTG	144.91498
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.552	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80218_Aamu_Bax2006_COI	MB32	1	BB80218_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Present	Sample	Aamu_Bax2006_COI	MB32	COI	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	GCACAACCGGGATCTTTACTTC	144.91483
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80218_Pcan_Bot2011_IGS	MB32	1	BB80218_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB32	IGS	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	CGTAATCCTCAGTACTGGCTA	144.91483
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.49763107	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80218_Sspa_Oph2007_COI	MB32	1	BB80218_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Present	Sample	Sspa_Oph2007_COI	MB32	COI	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	GAAGGCCTTCCGATGATC	144.91483
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.247	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80218_Upin_Bot2011_COI	MB32	1	BB80218_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Present	Sample	Upin_Bot2011_COI	MB32	COI	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	CTTTAATTACAGCGTTTTTATTGTTGT	144.91483
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80219_Mgig_Bot2012_COI	MB2	1	BB80219_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Absent	Sample	Mgig_Bot2012_COI	MB2	COI	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	TCTTATTCGTTGGAGACTTTATAACCCT	144.90381
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.069	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80221_Aamu_Bax2006_COI	MB7	1	BB80221_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Present	Sample	Aamu_Bax2006_COI	MB7	COI	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	GCACAACCGGGATCTTTACTTC	144.90603
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80221_Pcan_Bot2011_IGS	MB7	1	BB80221_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB7	IGS	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	CGTAATCCTCAGTACTGGCTA	144.90603
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.32262421	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80221_Sspa_Oph2007_COI	MB7	1	BB80221_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Present	Sample	Sspa_Oph2007_COI	MB7	COI	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	GAAGGCCTTCCGATGATC	144.90603
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.278	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80221_Upin_Bot2011_COI	MB7	1	BB80221_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Present	Sample	Upin_Bot2011_COI	MB7	COI	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	CTTTAATTACAGCGTTTTTATTGTTGT	144.90603
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80222_Mgig_Bot2012_COI	MB16	1	BB80222_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Absent	Sample	Mgig_Bot2012_COI	MB16	COI	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	TCTTATTCGTTGGAGACTTTATAACCCT	144.91447
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80223_Cmae_Bot2011_COI	MB13	1	BB80223_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Absent	Sample	Cmae_Bot2011_COI	MB13	COI	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	ATGAACAGTCTATCCTCCTTTAG	144.91033
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80223_Hsan_Wil2021_COI	MB13	1	BB80223_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Absent	Sample	Hsan_Wil2021_COI	MB13	COI	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	CCTGGGCCGGTATAGTAGGT	144.91033
BB80223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84745	WGS84	BB80223	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80223_Msal_Bot2012_COI	MB13	1	BB80223_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80223	Sample	BB80223	targeted	Absent	Sample	Msal_Bot2012_COI	MB13	COI	45	BB80223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB13	GYTAGTTCCRATGATGTTAGCTG	144.91033
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.836	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80225_Aamu_Bax2006_COI	MB11	1	BB80225_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Present	Sample	Aamu_Bax2006_COI	MB11	COI	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	GCACAACCGGGATCTTTACTTC	144.90635
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80225_Pcan_Bot2011_IGS	MB11	1	BB80225_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB11	IGS	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	CGTAATCCTCAGTACTGGCTA	144.90635
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.06782913	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80225_Sspa_Oph2007_COI	MB11	1	BB80225_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Present	Sample	Sspa_Oph2007_COI	MB11	COI	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	GAAGGCCTTCCGATGATC	144.90635
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.645	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80225_Upin_Bot2011_COI	MB11	1	BB80225_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Present	Sample	Upin_Bot2011_COI	MB11	COI	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	CTTTAATTACAGCGTTTTTATTGTTGT	144.90635
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80226_Mgig_Bot2012_COI	MB28	1	BB80226_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Absent	Sample	Mgig_Bot2012_COI	MB28	COI	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	TCTTATTCGTTGGAGACTTTATAACCCT	144.9324
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80227_Cmae_Bot2011_COI	MB26	1	BB80227_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Absent	Sample	Cmae_Bot2011_COI	MB26	COI	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	ATGAACAGTCTATCCTCCTTTAG	144.92895
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	45.51	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80227_Hsan_Wil2021_COI	MB26	1	BB80227_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Present	Sample	Hsan_Wil2021_COI	MB26	COI	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	CCTGGGCCGGTATAGTAGGT	144.92895
BB80227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84783	WGS84	BB80227	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80227_Msal_Bot2012_COI	MB26	1	BB80227_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80227	Sample	BB80227	targeted	Absent	Sample	Msal_Bot2012_COI	MB26	COI	45	BB80227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB26	GYTAGTTCCRATGATGTTAGCTG	144.92895
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.235	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80229_Aamu_Bax2006_COI	MB22	1	BB80229_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Present	Sample	Aamu_Bax2006_COI	MB22	COI	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	GCACAACCGGGATCTTTACTTC	144.92823
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80229_Pcan_Bot2011_IGS	MB22	1	BB80229_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB22	IGS	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	CGTAATCCTCAGTACTGGCTA	144.92823
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.33444214	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80229_Sspa_Oph2007_COI	MB22	1	BB80229_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Present	Sample	Sspa_Oph2007_COI	MB22	COI	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	GAAGGCCTTCCGATGATC	144.92823
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.685	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80229_Upin_Bot2011_COI	MB22	1	BB80229_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Present	Sample	Upin_Bot2011_COI	MB22	COI	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	CTTTAATTACAGCGTTTTTATTGTTGT	144.92823
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80230_Cmae_Bot2011_COI	MB18	1	BB80230_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Absent	Sample	Cmae_Bot2011_COI	MB18	COI	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	ATGAACAGTCTATCCTCCTTTAG	144.92605
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.831	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80230_Hsan_Wil2021_COI	MB18	1	BB80230_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Present	Sample	Hsan_Wil2021_COI	MB18	COI	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	CCTGGGCCGGTATAGTAGGT	144.92605
BB80230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8449	WGS84	BB80230	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80230_Msal_Bot2012_COI	MB18	1	BB80230_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80230	Sample	BB80230	targeted	Absent	Sample	Msal_Bot2012_COI	MB18	COI	45	BB80230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB18	GYTAGTTCCRATGATGTTAGCTG	144.92605
BB80232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88741	WGS84	BB80232	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.115	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80232_Aamu_Bax2006_COI	HB10	1	BB80232_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80232	Sample	BB80232	targeted	Present	Sample	Aamu_Bax2006_COI	HB10	COI	45	BB80232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB10	GCACAACCGGGATCTTTACTTC	147.34018
BB80232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88741	WGS84	BB80232	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80232_Pcan_Bot2011_IGS	HB10	1	BB80232_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80232	Sample	BB80232	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB10	IGS	45	BB80232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB10	CGTAATCCTCAGTACTGGCTA	147.34018
BB80232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88741	WGS84	BB80232	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80232_Sspa_Oph2007_COI	HB10	1	BB80232_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80232	Sample	BB80232	targeted	Absent	Sample	Sspa_Oph2007_COI	HB10	COI	45	BB80232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB10	GAAGGCCTTCCGATGATC	147.34018
BB80232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88741	WGS84	BB80232	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80232_Upin_Bot2011_COI	HB10	1	BB80232_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80232	Sample	BB80232	targeted	Absent	Sample	Upin_Bot2011_COI	HB10	COI	45	BB80232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB10	CTTTAATTACAGCGTTTTTATTGTTGT	147.34018
BB80233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88499	WGS84	BB80233	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80233_Mgig_Bot2012_COI	HB5	1	BB80233_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80233	Sample	BB80233	targeted	Absent	Sample	Mgig_Bot2012_COI	HB5	COI	45	BB80233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB5	TCTTATTCGTTGGAGACTTTATAACCCT	147.33665
BB80240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.885	WGS84	BB80240	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80240_Mgig_Bot2012_COI	HB8	1	BB80240_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80240	Sample	BB80240	targeted	Absent	Sample	Mgig_Bot2012_COI	HB8	COI	45	BB80240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB8	TCTTATTCGTTGGAGACTTTATAACCCT	147.33265
BB80241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88368	WGS84	BB80241	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80241_Cmae_Bot2011_COI	HB7	1	BB80241_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80241	Sample	BB80241	targeted	Absent	Sample	Cmae_Bot2011_COI	HB7	COI	45	BB80241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB7	ATGAACAGTCTATCCTCCTTTAG	147.33443
BB80241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88368	WGS84	BB80241	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80241_Msal_Bot2012_COI	HB7	1	BB80241_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80241	Sample	BB80241	targeted	Absent	Sample	Msal_Bot2012_COI	HB7	COI	45	BB80241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB7	GYTAGTTCCRATGATGTTAGCTG	147.33443
BB80243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88098	WGS84	BB80243	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.428	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80243_Aamu_Bax2006_COI	HB13	1	BB80243_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80243	Sample	BB80243	targeted	Present	Sample	Aamu_Bax2006_COI	HB13	COI	45	BB80243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB13	GCACAACCGGGATCTTTACTTC	147.34197
BB80243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88098	WGS84	BB80243	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80243_Pcan_Bot2011_IGS	HB13	1	BB80243_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80243	Sample	BB80243	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB13	IGS	45	BB80243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB13	CGTAATCCTCAGTACTGGCTA	147.34197
BB80243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88098	WGS84	BB80243	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80243_Sspa_Oph2007_COI	HB13	1	BB80243_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80243	Sample	BB80243	targeted	Absent	Sample	Sspa_Oph2007_COI	HB13	COI	45	BB80243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB13	GAAGGCCTTCCGATGATC	147.34197
BB80243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88098	WGS84	BB80243	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80243_Upin_Bot2011_COI	HB13	1	BB80243_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80243	Sample	BB80243	targeted	Absent	Sample	Upin_Bot2011_COI	HB13	COI	45	BB80243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB13	CTTTAATTACAGCGTTTTTATTGTTGT	147.34197
BB80244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88586	WGS84	BB80244	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80244_Mgig_Bot2012_COI	HB1	1	BB80244_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80244	Sample	BB80244	targeted	Absent	Sample	Mgig_Bot2012_COI	HB1	COI	45	BB80244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB1	TCTTATTCGTTGGAGACTTTATAACCCT	147.33887
BB80245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84503	WGS84	BB80245	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.605	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80245_Cmae_Bot2011_COI	HB21	1	BB80245_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80245	Sample	BB80245	targeted	Present	Sample	Cmae_Bot2011_COI	HB21	COI	45	BB80245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB21	ATGAACAGTCTATCCTCCTTTAG	147.32826
BB80245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84503	WGS84	BB80245	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80245_Msal_Bot2012_COI	HB21	1	BB80245_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80245	Sample	BB80245	targeted	Absent	Sample	Msal_Bot2012_COI	HB21	COI	45	BB80245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB21	GYTAGTTCCRATGATGTTAGCTG	147.32826
BB80247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84651	WGS84	BB80247	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.023	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80247_Aamu_Bax2006_COI	HB16	1	BB80247_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80247	Sample	BB80247	targeted	Present	Sample	Aamu_Bax2006_COI	HB16	COI	45	BB80247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB16	GCACAACCGGGATCTTTACTTC	147.32893
BB80247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84651	WGS84	BB80247	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80247_Pcan_Bot2011_IGS	HB16	1	BB80247_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80247	Sample	BB80247	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB16	IGS	45	BB80247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB16	CGTAATCCTCAGTACTGGCTA	147.32893
BB80247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84651	WGS84	BB80247	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80247_Sspa_Oph2007_COI	HB16	1	BB80247_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80247	Sample	BB80247	targeted	Absent	Sample	Sspa_Oph2007_COI	HB16	COI	45	BB80247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB16	GAAGGCCTTCCGATGATC	147.32893
BB80247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84651	WGS84	BB80247	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80247_Upin_Bot2011_COI	HB16	1	BB80247_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80247	Sample	BB80247	targeted	Absent	Sample	Upin_Bot2011_COI	HB16	COI	45	BB80247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB16	CTTTAATTACAGCGTTTTTATTGTTGT	147.32893
BB80248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84752	WGS84	BB80248	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80248_Mgig_Bot2012_COI	HB14	1	BB80248_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80248	Sample	BB80248	targeted	Absent	Sample	Mgig_Bot2012_COI	HB14	COI	45	BB80248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB14	TCTTATTCGTTGGAGACTTTATAACCCT	147.32763
BB80249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.83215	WGS84	BB80249	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80249_Cmae_Bot2011_COI	HB24	1	BB80249_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80249	Sample	BB80249	targeted	Absent	Sample	Cmae_Bot2011_COI	HB24	COI	45	BB80249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB24	ATGAACAGTCTATCCTCCTTTAG	147.32207
BB80249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.83215	WGS84	BB80249	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80249_Msal_Bot2012_COI	HB24	1	BB80249_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80249	Sample	BB80249	targeted	Absent	Sample	Msal_Bot2012_COI	HB24	COI	45	BB80249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB24	GYTAGTTCCRATGATGTTAGCTG	147.32207
BB80250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.82857	WGS84	BB80250	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.373	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80250_Aamu_Bax2006_COI	HB22	1	BB80250_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80250	Sample	BB80250	targeted	Present	Sample	Aamu_Bax2006_COI	HB22	COI	45	BB80250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB22	GCACAACCGGGATCTTTACTTC	147.31482
BB80250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.82857	WGS84	BB80250	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80250_Pcan_Bot2011_IGS	HB22	1	BB80250_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80250	Sample	BB80250	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB22	IGS	45	BB80250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB22	CGTAATCCTCAGTACTGGCTA	147.31482
BB80250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.82857	WGS84	BB80250	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80250_Sspa_Oph2007_COI	HB22	1	BB80250_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80250	Sample	BB80250	targeted	Absent	Sample	Sspa_Oph2007_COI	HB22	COI	45	BB80250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB22	GAAGGCCTTCCGATGATC	147.31482
BB80250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.82857	WGS84	BB80250	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80250_Upin_Bot2011_COI	HB22	1	BB80250_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80250	Sample	BB80250	targeted	Absent	Sample	Upin_Bot2011_COI	HB22	COI	45	BB80250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB22	CTTTAATTACAGCGTTTTTATTGTTGT	147.31482
BB80251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81897	WGS84	BB80251	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80251_Mgig_Bot2012_COI	GB110	1	BB80251_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80251	Sample	BB80251	targeted	Absent	Sample	Mgig_Bot2012_COI	GB110	COI	45	BB80251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB110	TCTTATTCGTTGGAGACTTTATAACCCT	151.23877
BB80252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79283	WGS84	BB80252	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80252_Cmae_Bot2011_COI	GB95	1	BB80252_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80252	Sample	BB80252	targeted	Absent	Sample	Cmae_Bot2011_COI	GB95	COI	45	BB80252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB95	ATGAACAGTCTATCCTCCTTTAG	151.21146
BB80252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79283	WGS84	BB80252	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80252_Msal_Bot2012_COI	GB95	1	BB80252_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80252	Sample	BB80252	targeted	Absent	Sample	Msal_Bot2012_COI	GB95	COI	45	BB80252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB95	GYTAGTTCCRATGATGTTAGCTG	151.21146
BB80254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78354	WGS84	BB80254	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80254_Aamu_Bax2006_COI	GB90	1	BB80254_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80254	Sample	BB80254	targeted	Absent	Sample	Aamu_Bax2006_COI	GB90	COI	45	BB80254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB90	GCACAACCGGGATCTTTACTTC	151.20098
BB80254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78354	WGS84	BB80254	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80254_Pcan_Bot2011_IGS	GB90	1	BB80254_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80254	Sample	BB80254	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB90	IGS	45	BB80254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB90	CGTAATCCTCAGTACTGGCTA	151.20098
BB80254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78354	WGS84	BB80254	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80254_Sspa_Oph2007_COI	GB90	1	BB80254_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80254	Sample	BB80254	targeted	Absent	Sample	Sspa_Oph2007_COI	GB90	COI	45	BB80254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB90	GAAGGCCTTCCGATGATC	151.20098
BB80254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78354	WGS84	BB80254	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80254_Upin_Bot2011_COI	GB90	1	BB80254_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80254	Sample	BB80254	targeted	Absent	Sample	Upin_Bot2011_COI	GB90	COI	45	BB80254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB90	CTTTAATTACAGCGTTTTTATTGTTGT	151.20098
BB80255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77796	WGS84	BB80255	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80255_Mgig_Bot2012_COI	GB85	1	BB80255_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80255	Sample	BB80255	targeted	Absent	Sample	Mgig_Bot2012_COI	GB85	COI	45	BB80255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB85	TCTTATTCGTTGGAGACTTTATAACCCT	151.19555
BB80256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81467	WGS84	BB80256	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80256_Cmae_Bot2011_COI	GB80	1	BB80256_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80256	Sample	BB80256	targeted	Absent	Sample	Cmae_Bot2011_COI	GB80	COI	45	BB80256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB80	ATGAACAGTCTATCCTCCTTTAG	151.23642
BB80256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81467	WGS84	BB80256	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80256_Msal_Bot2012_COI	GB80	1	BB80256_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80256	Sample	BB80256	targeted	Absent	Sample	Msal_Bot2012_COI	GB80	COI	45	BB80256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB80	GYTAGTTCCRATGATGTTAGCTG	151.23642
BB80258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78741	WGS84	BB80258	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80258_Aamu_Bax2006_COI	GB50	1	BB80258_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80258	Sample	BB80258	targeted	Absent	Sample	Aamu_Bax2006_COI	GB50	COI	45	BB80258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB50	GCACAACCGGGATCTTTACTTC	151.1737
BB80258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78741	WGS84	BB80258	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80258_Pcan_Bot2011_IGS	GB50	1	BB80258_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80258	Sample	BB80258	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB50	IGS	45	BB80258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB50	CGTAATCCTCAGTACTGGCTA	151.1737
BB80258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78741	WGS84	BB80258	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80258_Sspa_Oph2007_COI	GB50	1	BB80258_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80258	Sample	BB80258	targeted	Absent	Sample	Sspa_Oph2007_COI	GB50	COI	45	BB80258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB50	GAAGGCCTTCCGATGATC	151.1737
BB80258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78741	WGS84	BB80258	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80258_Upin_Bot2011_COI	GB50	1	BB80258_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80258	Sample	BB80258	targeted	Absent	Sample	Upin_Bot2011_COI	GB50	COI	45	BB80258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB50	CTTTAATTACAGCGTTTTTATTGTTGT	151.1737
BB80259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.7849	WGS84	BB80259	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80259_Mgig_Bot2012_COI	GB45	1	BB80259_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80259	Sample	BB80259	targeted	Absent	Sample	Mgig_Bot2012_COI	GB45	COI	45	BB80259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB45	TCTTATTCGTTGGAGACTTTATAACCCT	151.17149
BB80261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8056	WGS84	BB80261	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80261_Aamu_Bax2006_COI	GB35	1	BB80261_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80261	Sample	BB80261	targeted	Absent	Sample	Aamu_Bax2006_COI	GB35	COI	45	BB80261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB35	GCACAACCGGGATCTTTACTTC	151.21581
BB80261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8056	WGS84	BB80261	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80261_Pcan_Bot2011_IGS	GB35	1	BB80261_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80261	Sample	BB80261	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB35	IGS	45	BB80261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB35	CGTAATCCTCAGTACTGGCTA	151.21581
BB80261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8056	WGS84	BB80261	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80261_Sspa_Oph2007_COI	GB35	1	BB80261_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80261	Sample	BB80261	targeted	Absent	Sample	Sspa_Oph2007_COI	GB35	COI	45	BB80261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB35	GAAGGCCTTCCGATGATC	151.21581
BB80261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8056	WGS84	BB80261	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80261_Upin_Bot2011_COI	GB35	1	BB80261_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80261	Sample	BB80261	targeted	Absent	Sample	Upin_Bot2011_COI	GB35	COI	45	BB80261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB35	CTTTAATTACAGCGTTTTTATTGTTGT	151.21581
BB80262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83508	WGS84	BB80262	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80262_Mgig_Bot2012_COI	GB20	1	BB80262_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80262	Sample	BB80262	targeted	Absent	Sample	Mgig_Bot2012_COI	GB20	COI	45	BB80262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB20	TCTTATTCGTTGGAGACTTTATAACCCT	151.26929
BB80263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8365	WGS84	BB80263	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80263_Cmae_Bot2011_COI	GB15	1	BB80263_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80263	Sample	BB80263	targeted	Absent	Sample	Cmae_Bot2011_COI	GB15	COI	45	BB80263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB15	ATGAACAGTCTATCCTCCTTTAG	151.2728
BB80263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8365	WGS84	BB80263	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80263_Msal_Bot2012_COI	GB15	1	BB80263_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80263	Sample	BB80263	targeted	Absent	Sample	Msal_Bot2012_COI	GB15	COI	45	BB80263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB15	GYTAGTTCCRATGATGTTAGCTG	151.2728
BB80265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.853	WGS84	BB80265	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80265_Aamu_Bax2006_COI	GB5	1	BB80265_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80265	Sample	BB80265	targeted	Absent	Sample	Aamu_Bax2006_COI	GB5	COI	45	BB80265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB5	GCACAACCGGGATCTTTACTTC	151.32026
BB80265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.853	WGS84	BB80265	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80265_Pcan_Bot2011_IGS	GB5	1	BB80265_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80265	Sample	BB80265	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB5	IGS	45	BB80265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB5	CGTAATCCTCAGTACTGGCTA	151.32026
BB80265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.853	WGS84	BB80265	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80265_Sspa_Oph2007_COI	GB5	1	BB80265_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80265	Sample	BB80265	targeted	Absent	Sample	Sspa_Oph2007_COI	GB5	COI	45	BB80265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB5	GAAGGCCTTCCGATGATC	151.32026
BB80265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.853	WGS84	BB80265	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80265_Upin_Bot2011_COI	GB5	1	BB80265_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80265	Sample	BB80265	targeted	Absent	Sample	Upin_Bot2011_COI	GB5	COI	45	BB80265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB5	CTTTAATTACAGCGTTTTTATTGTTGT	151.32026
BB80272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80744	WGS84	BB80272	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80272_Aamu_Bax2006_COI	GB42	1	BB80272_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80272	Sample	BB80272	targeted	Absent	Sample	Aamu_Bax2006_COI	GB42	COI	45	BB80272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB42	GCACAACCGGGATCTTTACTTC	151.21814
BB80272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80744	WGS84	BB80272	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80272_Pcan_Bot2011_IGS	GB42	1	BB80272_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80272	Sample	BB80272	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB42	IGS	45	BB80272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB42	CGTAATCCTCAGTACTGGCTA	151.21814
BB80272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80744	WGS84	BB80272	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80272_Sspa_Oph2007_COI	GB42	1	BB80272_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80272	Sample	BB80272	targeted	Absent	Sample	Sspa_Oph2007_COI	GB42	COI	45	BB80272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB42	GAAGGCCTTCCGATGATC	151.21814
BB80272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80744	WGS84	BB80272	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80272_Upin_Bot2011_COI	GB42	1	BB80272_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80272	Sample	BB80272	targeted	Absent	Sample	Upin_Bot2011_COI	GB42	COI	45	BB80272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB42	CTTTAATTACAGCGTTTTTATTGTTGT	151.21814
BB80273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80713	WGS84	BB80273	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80273_Mgig_Bot2012_COI	GB38	1	BB80273_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80273	Sample	BB80273	targeted	Absent	Sample	Mgig_Bot2012_COI	GB38	COI	45	BB80273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB38	TCTTATTCGTTGGAGACTTTATAACCCT	151.21483
BB80274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80665	WGS84	BB80274	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80274_Cmae_Bot2011_COI	GB37	1	BB80274_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80274	Sample	BB80274	targeted	Absent	Sample	Cmae_Bot2011_COI	GB37	COI	45	BB80274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB37	ATGAACAGTCTATCCTCCTTTAG	151.2207
BB80274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80665	WGS84	BB80274	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80274_Msal_Bot2012_COI	GB37	1	BB80274_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80274	Sample	BB80274	targeted	Absent	Sample	Msal_Bot2012_COI	GB37	COI	45	BB80274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB37	GYTAGTTCCRATGATGTTAGCTG	151.2207
BB80276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79094	WGS84	BB80276	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80276_Aamu_Bax2006_COI	GB58	1	BB80276_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80276	Sample	BB80276	targeted	Absent	Sample	Aamu_Bax2006_COI	GB58	COI	45	BB80276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB58	GCACAACCGGGATCTTTACTTC	151.1826
BB80276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79094	WGS84	BB80276	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80276_Pcan_Bot2011_IGS	GB58	1	BB80276_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80276	Sample	BB80276	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB58	IGS	45	BB80276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB58	CGTAATCCTCAGTACTGGCTA	151.1826
BB80276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79094	WGS84	BB80276	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80276_Sspa_Oph2007_COI	GB58	1	BB80276_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80276	Sample	BB80276	targeted	Absent	Sample	Sspa_Oph2007_COI	GB58	COI	45	BB80276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB58	GAAGGCCTTCCGATGATC	151.1826
BB80276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79094	WGS84	BB80276	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80276_Upin_Bot2011_COI	GB58	1	BB80276_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80276	Sample	BB80276	targeted	Absent	Sample	Upin_Bot2011_COI	GB58	COI	45	BB80276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB58	CTTTAATTACAGCGTTTTTATTGTTGT	151.1826
BB80277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78985	WGS84	BB80277	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80277_Mgig_Bot2012_COI	GB57	1	BB80277_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80277	Sample	BB80277	targeted	Absent	Sample	Mgig_Bot2012_COI	GB57	COI	45	BB80277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB57	TCTTATTCGTTGGAGACTTTATAACCCT	151.18016
BB80278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78795	WGS84	BB80278	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80278_Cmae_Bot2011_COI	GB56	1	BB80278_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80278	Sample	BB80278	targeted	Absent	Sample	Cmae_Bot2011_COI	GB56	COI	45	BB80278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB56	ATGAACAGTCTATCCTCCTTTAG	151.17782
BB80278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78795	WGS84	BB80278	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80278_Msal_Bot2012_COI	GB56	1	BB80278_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80278	Sample	BB80278	targeted	Absent	Sample	Msal_Bot2012_COI	GB56	COI	45	BB80278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB56	GYTAGTTCCRATGATGTTAGCTG	151.17782
BB80280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78708	WGS84	BB80280	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80280_Mgig_Bot2012_COI	GB52	1	BB80280_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80280	Sample	BB80280	targeted	Absent	Sample	Mgig_Bot2012_COI	GB52	COI	45	BB80280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB52	TCTTATTCGTTGGAGACTTTATAACCCT	151.17666
BB80281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78569	WGS84	BB80281	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80281_Cmae_Bot2011_COI	GB48	1	BB80281_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80281	Sample	BB80281	targeted	Absent	Sample	Cmae_Bot2011_COI	GB48	COI	45	BB80281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB48	ATGAACAGTCTATCCTCCTTTAG	151.17473
BB80281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78569	WGS84	BB80281	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80281_Msal_Bot2012_COI	GB48	1	BB80281_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80281	Sample	BB80281	targeted	Absent	Sample	Msal_Bot2012_COI	GB48	COI	45	BB80281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB48	GYTAGTTCCRATGATGTTAGCTG	151.17473
BB80283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80283	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80283_Aamu_Bax2006_COI	GB92	1	BB80283_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80283	Sample	BB80283	targeted	Absent	Sample	Aamu_Bax2006_COI	GB92	COI	45	BB80283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB92	GCACAACCGGGATCTTTACTTC	151.20462
BB80283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80283	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80283_Pcan_Bot2011_IGS	GB92	1	BB80283_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80283	Sample	BB80283	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB92	IGS	45	BB80283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB92	CGTAATCCTCAGTACTGGCTA	151.20462
BB80283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80283	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80283_Sspa_Oph2007_COI	GB92	1	BB80283_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80283	Sample	BB80283	targeted	Absent	Sample	Sspa_Oph2007_COI	GB92	COI	45	BB80283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB92	GAAGGCCTTCCGATGATC	151.20462
BB80283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80283	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80283_Upin_Bot2011_COI	GB92	1	BB80283_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80283	Sample	BB80283	targeted	Absent	Sample	Upin_Bot2011_COI	GB92	COI	45	BB80283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB92	CTTTAATTACAGCGTTTTTATTGTTGT	151.20462
BB80284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78078	WGS84	BB80284	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80284_Mgig_Bot2012_COI	GB89	1	BB80284_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80284	Sample	BB80284	targeted	Absent	Sample	Mgig_Bot2012_COI	GB89	COI	45	BB80284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB89	TCTTATTCGTTGGAGACTTTATAACCCT	151.19976
BB80285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77979	WGS84	BB80285	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80285_Cmae_Bot2011_COI	GB87	1	BB80285_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80285	Sample	BB80285	targeted	Absent	Sample	Cmae_Bot2011_COI	GB87	COI	45	BB80285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB87	ATGAACAGTCTATCCTCCTTTAG	151.19725
BB80285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77979	WGS84	BB80285	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80285_Msal_Bot2012_COI	GB87	1	BB80285_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80285	Sample	BB80285	targeted	Absent	Sample	Msal_Bot2012_COI	GB87	COI	45	BB80285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB87	GYTAGTTCCRATGATGTTAGCTG	151.19725
BB80287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81718	WGS84	BB80287	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80287_Aamu_Bax2006_COI	GB84	1	BB80287_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80287	Sample	BB80287	targeted	Absent	Sample	Aamu_Bax2006_COI	GB84	COI	45	BB80287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB84	GCACAACCGGGATCTTTACTTC	151.23821
BB80287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81718	WGS84	BB80287	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80287_Pcan_Bot2011_IGS	GB84	1	BB80287_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80287	Sample	BB80287	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB84	IGS	45	BB80287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB84	CGTAATCCTCAGTACTGGCTA	151.23821
BB80287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81718	WGS84	BB80287	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80287_Sspa_Oph2007_COI	GB84	1	BB80287_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80287	Sample	BB80287	targeted	Absent	Sample	Sspa_Oph2007_COI	GB84	COI	45	BB80287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB84	GAAGGCCTTCCGATGATC	151.23821
BB80287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81718	WGS84	BB80287	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80287_Upin_Bot2011_COI	GB84	1	BB80287_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80287	Sample	BB80287	targeted	Absent	Sample	Upin_Bot2011_COI	GB84	COI	45	BB80287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB84	CTTTAATTACAGCGTTTTTATTGTTGT	151.23821
BB80288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82325	WGS84	BB80288	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80288_Mgig_Bot2012_COI	GB98	1	BB80288_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80288	Sample	BB80288	targeted	Absent	Sample	Mgig_Bot2012_COI	GB98	COI	45	BB80288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB98	TCTTATTCGTTGGAGACTTTATAACCCT	151.24116
BB80289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81519	WGS84	BB80289	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80289_Cmae_Bot2011_COI	GB79	1	BB80289_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80289	Sample	BB80289	targeted	Absent	Sample	Cmae_Bot2011_COI	GB79	COI	45	BB80289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB79	ATGAACAGTCTATCCTCCTTTAG	151.23887
BB80289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81519	WGS84	BB80289	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80289_Msal_Bot2012_COI	GB79	1	BB80289_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80289	Sample	BB80289	targeted	Absent	Sample	Msal_Bot2012_COI	GB79	COI	45	BB80289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB79	GYTAGTTCCRATGATGTTAGCTG	151.23887
BB80290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81831	WGS84	BB80290	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80290_Aamu_Bax2006_COI	GB77	1	BB80290_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80290	Sample	BB80290	targeted	Absent	Sample	Aamu_Bax2006_COI	GB77	COI	45	BB80290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB77	GCACAACCGGGATCTTTACTTC	151.24192
BB80290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81831	WGS84	BB80290	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80290_Pcan_Bot2011_IGS	GB77	1	BB80290_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80290	Sample	BB80290	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB77	IGS	45	BB80290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB77	CGTAATCCTCAGTACTGGCTA	151.24192
BB80290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81831	WGS84	BB80290	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80290_Sspa_Oph2007_COI	GB77	1	BB80290_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80290	Sample	BB80290	targeted	Absent	Sample	Sspa_Oph2007_COI	GB77	COI	45	BB80290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB77	GAAGGCCTTCCGATGATC	151.24192
BB80290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81831	WGS84	BB80290	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80290_Upin_Bot2011_COI	GB77	1	BB80290_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80290	Sample	BB80290	targeted	Absent	Sample	Upin_Bot2011_COI	GB77	COI	45	BB80290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB77	CTTTAATTACAGCGTTTTTATTGTTGT	151.24192
BB80291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82048	WGS84	BB80291	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80291_Mgig_Bot2012_COI	GB76	1	BB80291_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80291	Sample	BB80291	targeted	Absent	Sample	Mgig_Bot2012_COI	GB76	COI	45	BB80291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB76	TCTTATTCGTTGGAGACTTTATAACCCT	151.24452
BB80292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82116	WGS84	BB80292	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80292_Cmae_Bot2011_COI	GB74	1	BB80292_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80292	Sample	BB80292	targeted	Absent	Sample	Cmae_Bot2011_COI	GB74	COI	45	BB80292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB74	ATGAACAGTCTATCCTCCTTTAG	151.24266
BB80292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82116	WGS84	BB80292	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80292_Msal_Bot2012_COI	GB74	1	BB80292_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80292	Sample	BB80292	targeted	Absent	Sample	Msal_Bot2012_COI	GB74	COI	45	BB80292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB74	GYTAGTTCCRATGATGTTAGCTG	151.24266
BB80294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82273	WGS84	BB80294	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80294_Aamu_Bax2006_COI	GB69	1	BB80294_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80294	Sample	BB80294	targeted	Absent	Sample	Aamu_Bax2006_COI	GB69	COI	45	BB80294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB69	GCACAACCGGGATCTTTACTTC	151.24409
BB80294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82273	WGS84	BB80294	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80294_Pcan_Bot2011_IGS	GB69	1	BB80294_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80294	Sample	BB80294	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB69	IGS	45	BB80294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB69	CGTAATCCTCAGTACTGGCTA	151.24409
BB80294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82273	WGS84	BB80294	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80294_Sspa_Oph2007_COI	GB69	1	BB80294_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80294	Sample	BB80294	targeted	Absent	Sample	Sspa_Oph2007_COI	GB69	COI	45	BB80294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB69	GAAGGCCTTCCGATGATC	151.24409
BB80294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82273	WGS84	BB80294	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80294_Upin_Bot2011_COI	GB69	1	BB80294_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80294	Sample	BB80294	targeted	Absent	Sample	Upin_Bot2011_COI	GB69	COI	45	BB80294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB69	CTTTAATTACAGCGTTTTTATTGTTGT	151.24409
BB80295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8244	WGS84	BB80295	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80295_Mgig_Bot2012_COI	GB68	1	BB80295_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80295	Sample	BB80295	targeted	Absent	Sample	Mgig_Bot2012_COI	GB68	COI	45	BB80295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB68	TCTTATTCGTTGGAGACTTTATAACCCT	151.24366
BB80296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82437	WGS84	BB80296	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80296_Cmae_Bot2011_COI	GB66	1	BB80296_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80296	Sample	BB80296	targeted	Absent	Sample	Cmae_Bot2011_COI	GB66	COI	45	BB80296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB66	ATGAACAGTCTATCCTCCTTTAG	151.24506
BB80296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82437	WGS84	BB80296	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80296_Msal_Bot2012_COI	GB66	1	BB80296_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80296	Sample	BB80296	targeted	Absent	Sample	Msal_Bot2012_COI	GB66	COI	45	BB80296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB66	GYTAGTTCCRATGATGTTAGCTG	151.24506
BB80298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8268	WGS84	BB80298	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80298_Aamu_Bax2006_COI	GB34	1	BB80298_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80298	Sample	BB80298	targeted	Absent	Sample	Aamu_Bax2006_COI	GB34	COI	45	BB80298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB34	GCACAACCGGGATCTTTACTTC	151.24598
BB80298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8268	WGS84	BB80298	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80298_Pcan_Bot2011_IGS	GB34	1	BB80298_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80298	Sample	BB80298	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB34	IGS	45	BB80298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB34	CGTAATCCTCAGTACTGGCTA	151.24598
BB80298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8268	WGS84	BB80298	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80298_Sspa_Oph2007_COI	GB34	1	BB80298_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80298	Sample	BB80298	targeted	Absent	Sample	Sspa_Oph2007_COI	GB34	COI	45	BB80298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB34	GAAGGCCTTCCGATGATC	151.24598
BB80298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8268	WGS84	BB80298	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80298_Upin_Bot2011_COI	GB34	1	BB80298_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80298	Sample	BB80298	targeted	Absent	Sample	Upin_Bot2011_COI	GB34	COI	45	BB80298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB34	CTTTAATTACAGCGTTTTTATTGTTGT	151.24598
BB80299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82251	WGS84	BB80299	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80299_Mgig_Bot2012_COI	GB33	1	BB80299_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80299	Sample	BB80299	targeted	Absent	Sample	Mgig_Bot2012_COI	GB33	COI	45	BB80299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB33	TCTTATTCGTTGGAGACTTTATAACCCT	151.24644
BB80300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82617	WGS84	BB80300	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80300_Cmae_Bot2011_COI	GB31	1	BB80300_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80300	Sample	BB80300	targeted	Absent	Sample	Cmae_Bot2011_COI	GB31	COI	45	BB80300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB31	ATGAACAGTCTATCCTCCTTTAG	151.24826
BB80300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82617	WGS84	BB80300	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80300_Msal_Bot2012_COI	GB31	1	BB80300_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80300	Sample	BB80300	targeted	Absent	Sample	Msal_Bot2012_COI	GB31	COI	45	BB80300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB31	GYTAGTTCCRATGATGTTAGCTG	151.24826
BB80302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81859	WGS84	BB80302	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80302_Aamu_Bax2006_COI	GB109	1	BB80302_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80302	Sample	BB80302	targeted	Absent	Sample	Aamu_Bax2006_COI	GB109	COI	45	BB80302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB109	GCACAACCGGGATCTTTACTTC	151.23987
BB80302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81859	WGS84	BB80302	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80302_Pcan_Bot2011_IGS	GB109	1	BB80302_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80302	Sample	BB80302	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB109	IGS	45	BB80302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB109	CGTAATCCTCAGTACTGGCTA	151.23987
BB80302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81859	WGS84	BB80302	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80302_Sspa_Oph2007_COI	GB109	1	BB80302_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80302	Sample	BB80302	targeted	Absent	Sample	Sspa_Oph2007_COI	GB109	COI	45	BB80302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB109	GAAGGCCTTCCGATGATC	151.23987
BB80302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81859	WGS84	BB80302	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80302_Upin_Bot2011_COI	GB109	1	BB80302_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80302	Sample	BB80302	targeted	Absent	Sample	Upin_Bot2011_COI	GB109	COI	45	BB80302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB109	CTTTAATTACAGCGTTTTTATTGTTGT	151.23987
BB80303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82008	WGS84	BB80303	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80303_Mgig_Bot2012_COI	GB107	1	BB80303_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80303	Sample	BB80303	targeted	Absent	Sample	Mgig_Bot2012_COI	GB107	COI	45	BB80303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB107	TCTTATTCGTTGGAGACTTTATAACCCT	151.23915
BB80304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82071	WGS84	BB80304	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80304_Cmae_Bot2011_COI	GB104	1	BB80304_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80304	Sample	BB80304	targeted	Absent	Sample	Cmae_Bot2011_COI	GB104	COI	45	BB80304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB104	ATGAACAGTCTATCCTCCTTTAG	151.2411
BB80304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82071	WGS84	BB80304	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80304_Msal_Bot2012_COI	GB104	1	BB80304_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80304	Sample	BB80304	targeted	Absent	Sample	Msal_Bot2012_COI	GB104	COI	45	BB80304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB104	GYTAGTTCCRATGATGTTAGCTG	151.2411
BB80306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82155	WGS84	BB80306	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80306_Aamu_Bax2006_COI	GB102	1	BB80306_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80306	Sample	BB80306	targeted	Absent	Sample	Aamu_Bax2006_COI	GB102	COI	45	BB80306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB102	GCACAACCGGGATCTTTACTTC	151.24001
BB80306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82155	WGS84	BB80306	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80306_Pcan_Bot2011_IGS	GB102	1	BB80306_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80306	Sample	BB80306	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB102	IGS	45	BB80306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB102	CGTAATCCTCAGTACTGGCTA	151.24001
BB80306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82155	WGS84	BB80306	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80306_Sspa_Oph2007_COI	GB102	1	BB80306_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80306	Sample	BB80306	targeted	Absent	Sample	Sspa_Oph2007_COI	GB102	COI	45	BB80306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB102	GAAGGCCTTCCGATGATC	151.24001
BB80306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82155	WGS84	BB80306	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80306_Upin_Bot2011_COI	GB102	1	BB80306_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80306	Sample	BB80306	targeted	Absent	Sample	Upin_Bot2011_COI	GB102	COI	45	BB80306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB102	CTTTAATTACAGCGTTTTTATTGTTGT	151.24001
BB80307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79354	WGS84	BB80307	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80307_Mgig_Bot2012_COI	GB96	1	BB80307_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80307	Sample	BB80307	targeted	Absent	Sample	Mgig_Bot2012_COI	GB96	COI	45	BB80307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB96	TCTTATTCGTTGGAGACTTTATAACCCT	151.21271
BB80308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79074	WGS84	BB80308	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80308_Cmae_Bot2011_COI	GB93	1	BB80308_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80308	Sample	BB80308	targeted	Absent	Sample	Cmae_Bot2011_COI	GB93	COI	45	BB80308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB93	ATGAACAGTCTATCCTCCTTTAG	151.21098
BB80308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79074	WGS84	BB80308	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80308_Msal_Bot2012_COI	GB93	1	BB80308_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80308	Sample	BB80308	targeted	Absent	Sample	Msal_Bot2012_COI	GB93	COI	45	BB80308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB93	GYTAGTTCCRATGATGTTAGCTG	151.21098
BB80310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83125	WGS84	BB80310	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80310_Mgig_Bot2012_COI	GB27	1	BB80310_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80310	Sample	BB80310	targeted	Absent	Sample	Mgig_Bot2012_COI	GB27	COI	45	BB80310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB27	TCTTATTCGTTGGAGACTTTATAACCCT	151.25757
BB80311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83146	WGS84	BB80311	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80311_Cmae_Bot2011_COI	GB26	1	BB80311_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80311	Sample	BB80311	targeted	Absent	Sample	Cmae_Bot2011_COI	GB26	COI	45	BB80311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB26	ATGAACAGTCTATCCTCCTTTAG	151.2596
BB80311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83146	WGS84	BB80311	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80311_Msal_Bot2012_COI	GB26	1	BB80311_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80311	Sample	BB80311	targeted	Absent	Sample	Msal_Bot2012_COI	GB26	COI	45	BB80311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB26	GYTAGTTCCRATGATGTTAGCTG	151.2596
BB80313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83295	WGS84	BB80313	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80313_Aamu_Bax2006_COI	GB23	1	BB80313_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80313	Sample	BB80313	targeted	Absent	Sample	Aamu_Bax2006_COI	GB23	COI	45	BB80313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB23	GCACAACCGGGATCTTTACTTC	151.2651
BB80313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83295	WGS84	BB80313	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80313_Pcan_Bot2011_IGS	GB23	1	BB80313_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80313	Sample	BB80313	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB23	IGS	45	BB80313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB23	CGTAATCCTCAGTACTGGCTA	151.2651
BB80313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83295	WGS84	BB80313	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80313_Sspa_Oph2007_COI	GB23	1	BB80313_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80313	Sample	BB80313	targeted	Absent	Sample	Sspa_Oph2007_COI	GB23	COI	45	BB80313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB23	GAAGGCCTTCCGATGATC	151.2651
BB80313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83295	WGS84	BB80313	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80313_Upin_Bot2011_COI	GB23	1	BB80313_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80313	Sample	BB80313	targeted	Absent	Sample	Upin_Bot2011_COI	GB23	COI	45	BB80313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB23	CTTTAATTACAGCGTTTTTATTGTTGT	151.2651
BB80314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83452	WGS84	BB80314	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80314_Mgig_Bot2012_COI	GB22	1	BB80314_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80314	Sample	BB80314	targeted	Absent	Sample	Mgig_Bot2012_COI	GB22	COI	45	BB80314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB22	TCTTATTCGTTGGAGACTTTATAACCCT	151.267
BB80315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83503	WGS84	BB80315	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80315_Cmae_Bot2011_COI	GB18	1	BB80315_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80315	Sample	BB80315	targeted	Absent	Sample	Cmae_Bot2011_COI	GB18	COI	45	BB80315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB18	ATGAACAGTCTATCCTCCTTTAG	151.27135
BB80315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83503	WGS84	BB80315	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80315_Msal_Bot2012_COI	GB18	1	BB80315_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80315	Sample	BB80315	targeted	Absent	Sample	Msal_Bot2012_COI	GB18	COI	45	BB80315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB18	GYTAGTTCCRATGATGTTAGCTG	151.27135
BB80317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8531	WGS84	BB80317	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80317_Aamu_Bax2006_COI	GB12	1	BB80317_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80317	Sample	BB80317	targeted	Absent	Sample	Aamu_Bax2006_COI	GB12	COI	45	BB80317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB12	GCACAACCGGGATCTTTACTTC	151.30906
BB80317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8531	WGS84	BB80317	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80317_Pcan_Bot2011_IGS	GB12	1	BB80317_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80317	Sample	BB80317	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB12	IGS	45	BB80317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB12	CGTAATCCTCAGTACTGGCTA	151.30906
BB80317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8531	WGS84	BB80317	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80317_Sspa_Oph2007_COI	GB12	1	BB80317_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80317	Sample	BB80317	targeted	Absent	Sample	Sspa_Oph2007_COI	GB12	COI	45	BB80317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB12	GAAGGCCTTCCGATGATC	151.30906
BB80317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8531	WGS84	BB80317	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80317_Upin_Bot2011_COI	GB12	1	BB80317_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80317	Sample	BB80317	targeted	Absent	Sample	Upin_Bot2011_COI	GB12	COI	45	BB80317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB12	CTTTAATTACAGCGTTTTTATTGTTGT	151.30906
BB80318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85238	WGS84	BB80318	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80318_Mgig_Bot2012_COI	GB8	1	BB80318_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80318	Sample	BB80318	targeted	Absent	Sample	Mgig_Bot2012_COI	GB8	COI	45	BB80318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB8	TCTTATTCGTTGGAGACTTTATAACCCT	151.31641
BB80320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85326	WGS84	BB80320	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80320_Aamu_Bax2006_COI	GB6	1	BB80320_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80320	Sample	BB80320	targeted	Absent	Sample	Aamu_Bax2006_COI	GB6	COI	45	BB80320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB6	GCACAACCGGGATCTTTACTTC	151.31809
BB80320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85326	WGS84	BB80320	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80320_Pcan_Bot2011_IGS	GB6	1	BB80320_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80320	Sample	BB80320	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB6	IGS	45	BB80320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB6	CGTAATCCTCAGTACTGGCTA	151.31809
BB80320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85326	WGS84	BB80320	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80320_Sspa_Oph2007_COI	GB6	1	BB80320_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80320	Sample	BB80320	targeted	Absent	Sample	Sspa_Oph2007_COI	GB6	COI	45	BB80320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB6	GAAGGCCTTCCGATGATC	151.31809
BB80320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85326	WGS84	BB80320	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80320_Upin_Bot2011_COI	GB6	1	BB80320_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80320	Sample	BB80320	targeted	Absent	Sample	Upin_Bot2011_COI	GB6	COI	45	BB80320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB6	CTTTAATTACAGCGTTTTTATTGTTGT	151.31809
BB80478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40392	WGS84	BB80478	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80478_Aamu_Bax2006_COI	BB60	1	BB80478_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80478	Sample	BB80478	targeted	Absent	Sample	Aamu_Bax2006_COI	BB60	COI	45	BB80478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB60	GCACAACCGGGATCTTTACTTC	153.15318
BB80478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40392	WGS84	BB80478	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80478_Pcan_Bot2011_IGS	BB60	1	BB80478_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80478	Sample	BB80478	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB60	IGS	45	BB80478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB60	CGTAATCCTCAGTACTGGCTA	153.15318
BB80478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40392	WGS84	BB80478	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80478_Sspa_Oph2007_COI	BB60	1	BB80478_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80478	Sample	BB80478	targeted	Absent	Sample	Sspa_Oph2007_COI	BB60	COI	45	BB80478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB60	GAAGGCCTTCCGATGATC	153.15318
BB80478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40392	WGS84	BB80478	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80478_Upin_Bot2011_COI	BB60	1	BB80478_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80478	Sample	BB80478	targeted	Absent	Sample	Upin_Bot2011_COI	BB60	COI	45	BB80478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB60	CTTTAATTACAGCGTTTTTATTGTTGT	153.15318
BB80479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4006	WGS84	BB80479	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80479_Mgig_Bot2012_COI	BB25	1	BB80479_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80479	Sample	BB80479	targeted	Absent	Sample	Mgig_Bot2012_COI	BB25	COI	45	BB80479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB25	TCTTATTCGTTGGAGACTTTATAACCCT	153.16275
BB80481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38453	WGS84	BB80481	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80481_Aamu_Bax2006_COI	BB20	1	BB80481_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80481	Sample	BB80481	targeted	Absent	Sample	Aamu_Bax2006_COI	BB20	COI	45	BB80481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB20	GCACAACCGGGATCTTTACTTC	153.15992
BB80481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38453	WGS84	BB80481	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80481_Pcan_Bot2011_IGS	BB20	1	BB80481_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80481	Sample	BB80481	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB20	IGS	45	BB80481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB20	CGTAATCCTCAGTACTGGCTA	153.15992
BB80481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38453	WGS84	BB80481	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80481_Sspa_Oph2007_COI	BB20	1	BB80481_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80481	Sample	BB80481	targeted	Absent	Sample	Sspa_Oph2007_COI	BB20	COI	45	BB80481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB20	GAAGGCCTTCCGATGATC	153.15992
BB80481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38453	WGS84	BB80481	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80481_Upin_Bot2011_COI	BB20	1	BB80481_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80481	Sample	BB80481	targeted	Absent	Sample	Upin_Bot2011_COI	BB20	COI	45	BB80481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB20	CTTTAATTACAGCGTTTTTATTGTTGT	153.15992
BB80482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37816	WGS84	BB80482	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80482_Mgig_Bot2012_COI	BB15	1	BB80482_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80482	Sample	BB80482	targeted	Absent	Sample	Mgig_Bot2012_COI	BB15	COI	45	BB80482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB15	TCTTATTCGTTGGAGACTTTATAACCCT	153.16523
BB80483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41377	WGS84	BB80483	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80483_Cmae_Bot2011_COI	BB57	1	BB80483_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80483	Sample	BB80483	targeted	Absent	Sample	Cmae_Bot2011_COI	BB57	COI	45	BB80483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB57	ATGAACAGTCTATCCTCCTTTAG	153.14357
BB80483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41377	WGS84	BB80483	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80483_Msal_Bot2012_COI	BB57	1	BB80483_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80483	Sample	BB80483	targeted	Absent	Sample	Msal_Bot2012_COI	BB57	COI	45	BB80483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB57	GYTAGTTCCRATGATGTTAGCTG	153.14357
BB80485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42242	WGS84	BB80485	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80485_Aamu_Bax2006_COI	BB54	1	BB80485_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80485	Sample	BB80485	targeted	Absent	Sample	Aamu_Bax2006_COI	BB54	COI	45	BB80485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB54	GCACAACCGGGATCTTTACTTC	153.13425
BB80485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42242	WGS84	BB80485	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80485_Pcan_Bot2011_IGS	BB54	1	BB80485_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80485	Sample	BB80485	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB54	IGS	45	BB80485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB54	CGTAATCCTCAGTACTGGCTA	153.13425
BB80485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42242	WGS84	BB80485	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80485_Sspa_Oph2007_COI	BB54	1	BB80485_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80485	Sample	BB80485	targeted	Absent	Sample	Sspa_Oph2007_COI	BB54	COI	45	BB80485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB54	GAAGGCCTTCCGATGATC	153.13425
BB80485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42242	WGS84	BB80485	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80485_Upin_Bot2011_COI	BB54	1	BB80485_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80485	Sample	BB80485	targeted	Absent	Sample	Upin_Bot2011_COI	BB54	COI	45	BB80485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB54	CTTTAATTACAGCGTTTTTATTGTTGT	153.13425
BB80486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42478	WGS84	BB80486	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80486_Mgig_Bot2012_COI	BB53	1	BB80486_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80486	Sample	BB80486	targeted	Absent	Sample	Mgig_Bot2012_COI	BB53	COI	45	BB80486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB53	TCTTATTCGTTGGAGACTTTATAACCCT	153.12951
BB80487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42763	WGS84	BB80487	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80487_Cmae_Bot2011_COI	BB52	1	BB80487_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80487	Sample	BB80487	targeted	Absent	Sample	Cmae_Bot2011_COI	BB52	COI	45	BB80487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB52	ATGAACAGTCTATCCTCCTTTAG	153.12369
BB80487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42763	WGS84	BB80487	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80487_Msal_Bot2012_COI	BB52	1	BB80487_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80487	Sample	BB80487	targeted	Absent	Sample	Msal_Bot2012_COI	BB52	COI	45	BB80487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB52	GYTAGTTCCRATGATGTTAGCTG	153.12369
BB80489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.43049	WGS84	BB80489	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80489_Aamu_Bax2006_COI	BB44	1	BB80489_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80489	Sample	BB80489	targeted	Absent	Sample	Aamu_Bax2006_COI	BB44	COI	45	BB80489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB44	GCACAACCGGGATCTTTACTTC	153.11875
BB80489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.43049	WGS84	BB80489	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80489_Pcan_Bot2011_IGS	BB44	1	BB80489_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80489	Sample	BB80489	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB44	IGS	45	BB80489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB44	CGTAATCCTCAGTACTGGCTA	153.11875
BB80489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.43049	WGS84	BB80489	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80489_Sspa_Oph2007_COI	BB44	1	BB80489_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80489	Sample	BB80489	targeted	Absent	Sample	Sspa_Oph2007_COI	BB44	COI	45	BB80489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB44	GAAGGCCTTCCGATGATC	153.11875
BB80489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.43049	WGS84	BB80489	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80489_Upin_Bot2011_COI	BB44	1	BB80489_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80489	Sample	BB80489	targeted	Absent	Sample	Upin_Bot2011_COI	BB44	COI	45	BB80489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB44	CTTTAATTACAGCGTTTTTATTGTTGT	153.11875
BB80490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40702	WGS84	BB80490	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80490_Cmae_Bot2011_COI	BB62	1	BB80490_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80490	Sample	BB80490	targeted	Absent	Sample	Cmae_Bot2011_COI	BB62	COI	45	BB80490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB62	ATGAACAGTCTATCCTCCTTTAG	153.15063
BB80490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40702	WGS84	BB80490	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80490_Msal_Bot2012_COI	BB62	1	BB80490_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80490	Sample	BB80490	targeted	Absent	Sample	Msal_Bot2012_COI	BB62	COI	45	BB80490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB62	GYTAGTTCCRATGATGTTAGCTG	153.15063
BB80492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40135	WGS84	BB80492	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80492_Aamu_Bax2006_COI	BB27	1	BB80492_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80492	Sample	BB80492	targeted	Absent	Sample	Aamu_Bax2006_COI	BB27	COI	45	BB80492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB27	GCACAACCGGGATCTTTACTTC	153.16103
BB80492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40135	WGS84	BB80492	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80492_Pcan_Bot2011_IGS	BB27	1	BB80492_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80492	Sample	BB80492	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB27	IGS	45	BB80492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB27	CGTAATCCTCAGTACTGGCTA	153.16103
BB80492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40135	WGS84	BB80492	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80492_Sspa_Oph2007_COI	BB27	1	BB80492_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80492	Sample	BB80492	targeted	Absent	Sample	Sspa_Oph2007_COI	BB27	COI	45	BB80492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB27	GAAGGCCTTCCGATGATC	153.16103
BB80492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40135	WGS84	BB80492	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80492_Upin_Bot2011_COI	BB27	1	BB80492_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80492	Sample	BB80492	targeted	Absent	Sample	Upin_Bot2011_COI	BB27	COI	45	BB80492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB27	CTTTAATTACAGCGTTTTTATTGTTGT	153.16103
BB80493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44583	WGS84	BB80493	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80493_Mgig_Bot2012_COI	BB42	1	BB80493_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80493	Sample	BB80493	targeted	Absent	Sample	Mgig_Bot2012_COI	BB42	COI	45	BB80493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB42	TCTTATTCGTTGGAGACTTTATAACCCT	153.08345
BB80494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44631	WGS84	BB80494	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80494_Cmae_Bot2011_COI	BB39	1	BB80494_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80494	Sample	BB80494	targeted	Absent	Sample	Cmae_Bot2011_COI	BB39	COI	45	BB80494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB39	ATGAACAGTCTATCCTCCTTTAG	153.0775
BB80494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44631	WGS84	BB80494	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80494_Msal_Bot2012_COI	BB39	1	BB80494_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80494	Sample	BB80494	targeted	Absent	Sample	Msal_Bot2012_COI	BB39	COI	45	BB80494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB39	GYTAGTTCCRATGATGTTAGCTG	153.0775
BB80496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44558	WGS84	BB80496	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80496_Aamu_Bax2006_COI	BB36	1	BB80496_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80496	Sample	BB80496	targeted	Absent	Sample	Aamu_Bax2006_COI	BB36	COI	45	BB80496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB36	GCACAACCGGGATCTTTACTTC	153.07838
BB80496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44558	WGS84	BB80496	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80496_Pcan_Bot2011_IGS	BB36	1	BB80496_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80496	Sample	BB80496	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB36	IGS	45	BB80496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB36	CGTAATCCTCAGTACTGGCTA	153.07838
BB80496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44558	WGS84	BB80496	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80496_Sspa_Oph2007_COI	BB36	1	BB80496_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80496	Sample	BB80496	targeted	Absent	Sample	Sspa_Oph2007_COI	BB36	COI	45	BB80496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB36	GAAGGCCTTCCGATGATC	153.07838
BB80496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44558	WGS84	BB80496	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80496_Upin_Bot2011_COI	BB36	1	BB80496_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80496	Sample	BB80496	targeted	Absent	Sample	Upin_Bot2011_COI	BB36	COI	45	BB80496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB36	CTTTAATTACAGCGTTTTTATTGTTGT	153.07838
BB80497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44567	WGS84	BB80497	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80497_Mgig_Bot2012_COI	BB34	1	BB80497_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80497	Sample	BB80497	targeted	Absent	Sample	Mgig_Bot2012_COI	BB34	COI	45	BB80497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB34	TCTTATTCGTTGGAGACTTTATAACCCT	153.07495
BB80498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44397	WGS84	BB80498	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80498_Cmae_Bot2011_COI	BB31	1	BB80498_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80498	Sample	BB80498	targeted	Absent	Sample	Cmae_Bot2011_COI	BB31	COI	45	BB80498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB31	ATGAACAGTCTATCCTCCTTTAG	153.07629
BB80498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44397	WGS84	BB80498	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80498_Msal_Bot2012_COI	BB31	1	BB80498_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80498	Sample	BB80498	targeted	Absent	Sample	Msal_Bot2012_COI	BB31	COI	45	BB80498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB31	GYTAGTTCCRATGATGTTAGCTG	153.07629
BB80500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.3698	WGS84	BB80500	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80500_Aamu_Bax2006_COI	BB9	1	BB80500_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80500	Sample	BB80500	targeted	Absent	Sample	Aamu_Bax2006_COI	BB9	COI	45	BB80500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB9	GCACAACCGGGATCTTTACTTC	153.17124
BB80500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.3698	WGS84	BB80500	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80500_Pcan_Bot2011_IGS	BB9	1	BB80500_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80500	Sample	BB80500	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB9	IGS	45	BB80500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB9	CGTAATCCTCAGTACTGGCTA	153.17124
BB80500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.3698	WGS84	BB80500	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80500_Sspa_Oph2007_COI	BB9	1	BB80500_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80500	Sample	BB80500	targeted	Absent	Sample	Sspa_Oph2007_COI	BB9	COI	45	BB80500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB9	GAAGGCCTTCCGATGATC	153.17124
BB80500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.3698	WGS84	BB80500	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80500_Upin_Bot2011_COI	BB9	1	BB80500_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80500	Sample	BB80500	targeted	Absent	Sample	Upin_Bot2011_COI	BB9	COI	45	BB80500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB9	CTTTAATTACAGCGTTTTTATTGTTGT	153.17124
BB80501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36806	WGS84	BB80501	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80501_Mgig_Bot2012_COI	BB8	1	BB80501_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80501	Sample	BB80501	targeted	Absent	Sample	Mgig_Bot2012_COI	BB8	COI	45	BB80501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB8	TCTTATTCGTTGGAGACTTTATAACCCT	153.17231
BB80502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36487	WGS84	BB80502	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80502_Cmae_Bot2011_COI	BB6	1	BB80502_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80502	Sample	BB80502	targeted	Absent	Sample	Cmae_Bot2011_COI	BB6	COI	45	BB80502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB6	ATGAACAGTCTATCCTCCTTTAG	153.1748
BB80502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36487	WGS84	BB80502	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80502_Msal_Bot2012_COI	BB6	1	BB80502_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80502	Sample	BB80502	targeted	Absent	Sample	Msal_Bot2012_COI	BB6	COI	45	BB80502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB6	GYTAGTTCCRATGATGTTAGCTG	153.1748
BB80504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.39048	WGS84	BB80504	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80504_Aamu_Bax2006_COI	BB24	1	BB80504_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80504	Sample	BB80504	targeted	Absent	Sample	Aamu_Bax2006_COI	BB24	COI	45	BB80504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB24	GCACAACCGGGATCTTTACTTC	153.15714
BB80504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.39048	WGS84	BB80504	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80504_Pcan_Bot2011_IGS	BB24	1	BB80504_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80504	Sample	BB80504	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB24	IGS	45	BB80504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB24	CGTAATCCTCAGTACTGGCTA	153.15714
BB80504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.39048	WGS84	BB80504	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80504_Sspa_Oph2007_COI	BB24	1	BB80504_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80504	Sample	BB80504	targeted	Absent	Sample	Sspa_Oph2007_COI	BB24	COI	45	BB80504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB24	GAAGGCCTTCCGATGATC	153.15714
BB80504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.39048	WGS84	BB80504	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80504_Upin_Bot2011_COI	BB24	1	BB80504_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80504	Sample	BB80504	targeted	Absent	Sample	Upin_Bot2011_COI	BB24	COI	45	BB80504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB24	CTTTAATTACAGCGTTTTTATTGTTGT	153.15714
BB87783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84762	WGS84	BB87783	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87783_Cmae_Bot2011_COI	T11-01	1	BB87783_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87783	Sample	BB87783	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-01	COI	45	BB87783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-01	ATGAACAGTCTATCCTCCTTTAG	147.87521
BB87785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85535	WGS84	BB87785	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87785_Aamu_Bax2006_COI	T11-03	1	BB87785_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87785	Sample	BB87785	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-03	COI	45	BB87785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-03	GCACAACCGGGATCTTTACTTC	147.87278
BB87785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85535	WGS84	BB87785	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87785_Pcan_Bot2011_IGS	T11-03	1	BB87785_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87785	Sample	BB87785	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-03	IGS	45	BB87785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-03	CGTAATCCTCAGTACTGGCTA	147.87278
BB87785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85535	WGS84	BB87785	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87785_Sspa_Oph2007_COI	T11-03	1	BB87785_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87785	Sample	BB87785	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-03	COI	45	BB87785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-03	GAAGGCCTTCCGATGATC	147.87278
BB87785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85535	WGS84	BB87785	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87785_Upin_Bot2011_COI	T11-03	1	BB87785_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87785	Sample	BB87785	targeted	Absent	Sample	Upin_Bot2011_COI	T11-03	COI	45	BB87785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.87278
BB87786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88943	WGS84	BB87786	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87786_Cjap_Sim2018_COI	T11-04	1	BB87786_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87786	Sample	BB87786	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-04	COI	45	BB87786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81458
BB87786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88943	WGS84	BB87786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.067	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	27/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87786_Mgig_Bot2012_COI	T11-04	1	BB87786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87786	Sample	BB87786	targeted	Present	Sample	Mgig_Bot2012_COI	T11-04	COI	45	BB87786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.81458
BB87787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96278	WGS84	BB87787	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87787_Cmae_Bot2011_COI	T11-05	1	BB87787_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87787	Sample	BB87787	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-05	COI	45	BB87787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-05	ATGAACAGTCTATCCTCCTTTAG	147.53883
BB87789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96348	WGS84	BB87789	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.457	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87789_Aamu_Bax2006_COI	T11-07	1	BB87789_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87789	Sample	BB87789	targeted	Present	Sample	Aamu_Bax2006_COI	T11-07	COI	45	BB87789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-07	GCACAACCGGGATCTTTACTTC	147.51931
BB87789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96348	WGS84	BB87789	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87789_Pcan_Bot2011_IGS	T11-07	1	BB87789_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87789	Sample	BB87789	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-07	IGS	45	BB87789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-07	CGTAATCCTCAGTACTGGCTA	147.51931
BB87789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96348	WGS84	BB87789	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87789_Sspa_Oph2007_COI	T11-07	1	BB87789_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87789	Sample	BB87789	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-07	COI	45	BB87789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-07	GAAGGCCTTCCGATGATC	147.51931
BB87789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96348	WGS84	BB87789	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87789_Upin_Bot2011_COI	T11-07	1	BB87789_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87789	Sample	BB87789	targeted	Absent	Sample	Upin_Bot2011_COI	T11-07	COI	45	BB87789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.51931
BB87790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82515	WGS84	BB87790	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87790_Cmae_Bot2011_COI	T11-08	1	BB87790_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87790	Sample	BB87790	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-08	COI	45	BB87790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-08	ATGAACAGTCTATCCTCCTTTAG	147.3078
BB87792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82887	WGS84	BB87792	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87792_Aamu_Bax2006_COI	T11-10	1	BB87792_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87792	Sample	BB87792	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-10	COI	45	BB87792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-10	GCACAACCGGGATCTTTACTTC	147.30192
BB87792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82887	WGS84	BB87792	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87792_Pcan_Bot2011_IGS	T11-10	1	BB87792_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87792	Sample	BB87792	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-10	IGS	45	BB87792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-10	CGTAATCCTCAGTACTGGCTA	147.30192
BB87792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82887	WGS84	BB87792	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87792_Sspa_Oph2007_COI	T11-10	1	BB87792_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87792	Sample	BB87792	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-10	COI	45	BB87792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-10	GAAGGCCTTCCGATGATC	147.30192
BB87792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82887	WGS84	BB87792	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87792_Upin_Bot2011_COI	T11-10	1	BB87792_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87792	Sample	BB87792	targeted	Absent	Sample	Upin_Bot2011_COI	T11-10	COI	45	BB87792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.30192
BB87793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87793	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87793_Cjap_Sim2018_COI	T11-11	1	BB87793_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87793	Sample	BB87793	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-11	COI	45	BB87793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33688
BB87793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88552	WGS84	BB87793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.226	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87793_Mgig_Bot2012_COI	T11-11	1	BB87793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87793	Sample	BB87793	targeted	Present	Sample	Mgig_Bot2012_COI	T11-11	COI	45	BB87793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33688
BB87794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88265	WGS84	BB87794	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87794_Cmae_Bot2011_COI	T11-13	1	BB87794_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87794	Sample	BB87794	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-13	COI	45	BB87794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-13	ATGAACAGTCTATCCTCCTTTAG	147.33628
BB87796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88082	WGS84	BB87796	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.432	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87796_Aamu_Bax2006_COI	T11-14	1	BB87796_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87796	Sample	BB87796	targeted	Present	Sample	Aamu_Bax2006_COI	T11-14	COI	45	BB87796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-14	GCACAACCGGGATCTTTACTTC	147.3422
BB87796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88082	WGS84	BB87796	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87796_Pcan_Bot2011_IGS	T11-14	1	BB87796_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87796	Sample	BB87796	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-14	IGS	45	BB87796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-14	CGTAATCCTCAGTACTGGCTA	147.3422
BB87796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88082	WGS84	BB87796	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87796_Sspa_Oph2007_COI	T11-14	1	BB87796_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87796	Sample	BB87796	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-14	COI	45	BB87796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-14	GAAGGCCTTCCGATGATC	147.3422
BB87796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88082	WGS84	BB87796	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87796_Upin_Bot2011_COI	T11-14	1	BB87796_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87796	Sample	BB87796	targeted	Absent	Sample	Upin_Bot2011_COI	T11-14	COI	45	BB87796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.3422
BB87797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87836	WGS84	BB87797	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87797_Cjap_Sim2018_COI	T11-15	1	BB87797_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87797	Sample	BB87797	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-15	COI	45	BB87797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34177
BB87797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87836	WGS84	BB87797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.059	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87797_Mgig_Bot2012_COI	T11-15	1	BB87797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87797	Sample	BB87797	targeted	Present	Sample	Mgig_Bot2012_COI	T11-15	COI	45	BB87797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.34177
BB87798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87305	WGS84	BB87798	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87798_Cmae_Bot2011_COI	T11-16	1	BB87798_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87798	Sample	BB87798	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-16	COI	45	BB87798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-16	ATGAACAGTCTATCCTCCTTTAG	147.3363
BB87800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87800	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87800_Aamu_Bax2006_COI	S7-03	1	BB87800_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87800	Sample	BB87800	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-03	COI	45	BB87800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-03	GCACAACCGGGATCTTTACTTC	138.49696
BB87800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87800	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87800_Pcan_Bot2011_IGS	S7-03	1	BB87800_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87800	Sample	BB87800	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-03	IGS	45	BB87800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-03	CGTAATCCTCAGTACTGGCTA	138.49696
BB87800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87800	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.608	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87800_Sspa_Oph2007_COI	S7-03	1	BB87800_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87800	Sample	BB87800	targeted	Present	Sample	Sspa_Oph2007_COI	S7-03	COI	45	BB87800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-03	GAAGGCCTTCCGATGATC	138.49696
BB87800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87800	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87800_Upin_Bot2011_COI	S7-03	1	BB87800_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87800	Sample	BB87800	targeted	Absent	Sample	Upin_Bot2011_COI	S7-03	COI	45	BB87800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.49696
BB87804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83704	WGS84	BB87804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87804_Aamu_Bax2006_COI	S7-07	1	BB87804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87804	Sample	BB87804	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-07	COI	45	BB87804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-07	GCACAACCGGGATCTTTACTTC	138.50986
BB87804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83704	WGS84	BB87804	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87804_Pcan_Bot2011_IGS	S7-07	1	BB87804_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87804	Sample	BB87804	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-07	IGS	45	BB87804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-07	CGTAATCCTCAGTACTGGCTA	138.50986
BB87804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83704	WGS84	BB87804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.563	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87804_Sspa_Oph2007_COI	S7-07	1	BB87804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87804	Sample	BB87804	targeted	Present	Sample	Sspa_Oph2007_COI	S7-07	COI	45	BB87804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-07	GAAGGCCTTCCGATGATC	138.50986
BB87804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83704	WGS84	BB87804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87804_Upin_Bot2011_COI	S7-07	1	BB87804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87804	Sample	BB87804	targeted	Absent	Sample	Upin_Bot2011_COI	S7-07	COI	45	BB87804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.50986
BB87805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87805	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87805_Cjap_Sim2018_COI	S7-08	1	BB87805_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87805	Sample	BB87805	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-08	COI	45	BB87805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51031
BB87805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83703	WGS84	BB87805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.015	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87805_Mgig_Bot2012_COI	S7-08	1	BB87805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87805	Sample	BB87805	targeted	Present	Sample	Mgig_Bot2012_COI	S7-08	COI	45	BB87805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51031
BB87808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78381	WGS84	BB87808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87808_Aamu_Bax2006_COI	S7-11	1	BB87808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87808	Sample	BB87808	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-11	COI	45	BB87808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-11	GCACAACCGGGATCTTTACTTC	138.51578
BB87808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78381	WGS84	BB87808	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87808_Pcan_Bot2011_IGS	S7-11	1	BB87808_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87808	Sample	BB87808	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-11	IGS	45	BB87808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-11	CGTAATCCTCAGTACTGGCTA	138.51578
BB87808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78381	WGS84	BB87808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.936	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87808_Sspa_Oph2007_COI	S7-11	1	BB87808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87808	Sample	BB87808	targeted	Present	Sample	Sspa_Oph2007_COI	S7-11	COI	45	BB87808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-11	GAAGGCCTTCCGATGATC	138.51578
BB87808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78381	WGS84	BB87808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87808_Upin_Bot2011_COI	S7-11	1	BB87808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87808	Sample	BB87808	targeted	Absent	Sample	Upin_Bot2011_COI	S7-11	COI	45	BB87808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.51578
BB87809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78326	WGS84	BB87809	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87809_Cjap_Sim2018_COI	S7-12	1	BB87809_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87809	Sample	BB87809	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-12	COI	45	BB87809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51601
BB87809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78326	WGS84	BB87809	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87809_Mgig_Bot2012_COI	S7-12	1	BB87809_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87809	Sample	BB87809	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-12	COI	45	BB87809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.51601
BB87813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77223	WGS84	BB87813	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.701	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87813_Cmae_Bot2011_COI	S7-16	1	BB87813_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87813	Sample	BB87813	targeted	Present	Sample	Cmae_Bot2011_COI	S7-16	COI	45	BB87813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-16	ATGAACAGTCTATCCTCCTTTAG	138.48596
BB87815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77396	WGS84	BB87815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87815_Aamu_Bax2006_COI	S7-18	1	BB87815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87815	Sample	BB87815	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-18	COI	45	BB87815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-18	GCACAACCGGGATCTTTACTTC	138.486
BB87815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77396	WGS84	BB87815	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87815_Pcan_Bot2011_IGS	S7-18	1	BB87815_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87815	Sample	BB87815	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-18	IGS	45	BB87815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-18	CGTAATCCTCAGTACTGGCTA	138.486
BB87815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77396	WGS84	BB87815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87815_Sspa_Oph2007_COI	S7-18	1	BB87815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87815	Sample	BB87815	targeted	Absent	Sample	Sspa_Oph2007_COI	S7-18	COI	45	BB87815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-18	GAAGGCCTTCCGATGATC	138.486
BB87815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77396	WGS84	BB87815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87815_Upin_Bot2011_COI	S7-18	1	BB87815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87815	Sample	BB87815	targeted	Absent	Sample	Upin_Bot2011_COI	S7-18	COI	45	BB87815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.486
BB87819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80999	WGS84	BB87819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87819_Aamu_Bax2006_COI	S7-22	1	BB87819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87819	Sample	BB87819	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-22	COI	45	BB87819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-22	GCACAACCGGGATCTTTACTTC	138.51976
BB87819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80999	WGS84	BB87819	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87819_Pcan_Bot2011_IGS	S7-22	1	BB87819_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87819	Sample	BB87819	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-22	IGS	45	BB87819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-22	CGTAATCCTCAGTACTGGCTA	138.51976
BB87819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80999	WGS84	BB87819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.318	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87819_Sspa_Oph2007_COI	S7-22	1	BB87819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87819	Sample	BB87819	targeted	Present	Sample	Sspa_Oph2007_COI	S7-22	COI	45	BB87819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-22	GAAGGCCTTCCGATGATC	138.51976
BB87819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80999	WGS84	BB87819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87819_Upin_Bot2011_COI	S7-22	1	BB87819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87819	Sample	BB87819	targeted	Absent	Sample	Upin_Bot2011_COI	S7-22	COI	45	BB87819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-22	CTTTAATTACAGCGTTTTTATTGTTGT	138.51976
BB87822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81158	WGS84	BB87822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87822_Aamu_Bax2006_COI	S7-25	1	BB87822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87822	Sample	BB87822	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-25	COI	45	BB87822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-25	GCACAACCGGGATCTTTACTTC	138.52358
BB87822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81158	WGS84	BB87822	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87822_Pcan_Bot2011_IGS	S7-25	1	BB87822_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87822	Sample	BB87822	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-25	IGS	45	BB87822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-25	CGTAATCCTCAGTACTGGCTA	138.52358
BB87822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81158	WGS84	BB87822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.605	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87822_Sspa_Oph2007_COI	S7-25	1	BB87822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87822	Sample	BB87822	targeted	Present	Sample	Sspa_Oph2007_COI	S7-25	COI	45	BB87822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-25	GAAGGCCTTCCGATGATC	138.52358
BB87822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81158	WGS84	BB87822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87822_Upin_Bot2011_COI	S7-25	1	BB87822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87822	Sample	BB87822	targeted	Absent	Sample	Upin_Bot2011_COI	S7-25	COI	45	BB87822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-25	CTTTAATTACAGCGTTTTTATTGTTGT	138.52358
BB87823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84719	WGS84	BB87823	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87823_Cjap_Sim2018_COI	S7-01	1	BB87823_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87823	Sample	BB87823	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-01	COI	45	BB87823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4975
BB87823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84719	WGS84	BB87823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.008	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87823_Mgig_Bot2012_COI	S7-01	1	BB87823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87823	Sample	BB87823	targeted	Present	Sample	Mgig_Bot2012_COI	S7-01	COI	45	BB87823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.4975
BB87824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84768	WGS84	BB87824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87824_Cmae_Bot2011_COI	T12-01	1	BB87824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87824	Sample	BB87824	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-01	COI	45	BB87824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-01	ATGAACAGTCTATCCTCCTTTAG	147.8754
BB87826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85596	WGS84	BB87826	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87826_Aamu_Bax2006_COI	T12-03	1	BB87826_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87826	Sample	BB87826	targeted	Absent	Sample	Aamu_Bax2006_COI	T12-03	COI	45	BB87826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-03	GCACAACCGGGATCTTTACTTC	147.87236
BB87826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85596	WGS84	BB87826	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87826_Pcan_Bot2011_IGS	T12-03	1	BB87826_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87826	Sample	BB87826	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-03	IGS	45	BB87826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-03	CGTAATCCTCAGTACTGGCTA	147.87236
BB87826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85596	WGS84	BB87826	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87826_Sspa_Oph2007_COI	T12-03	1	BB87826_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87826	Sample	BB87826	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-03	COI	45	BB87826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-03	GAAGGCCTTCCGATGATC	147.87236
BB87826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85596	WGS84	BB87826	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.147	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87826_Upin_Bot2011_COI	T12-03	1	BB87826_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87826	Sample	BB87826	targeted	Present	Sample	Upin_Bot2011_COI	T12-03	COI	45	BB87826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.87236
BB87827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88956	WGS84	BB87827	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87827_Cjap_Sim2018_COI	T12-04	1	BB87827_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87827	Sample	BB87827	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-04	COI	45	BB87827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81287
BB87827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88956	WGS84	BB87827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.141	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	28/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87827_Mgig_Bot2012_COI	T12-04	1	BB87827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87827	Sample	BB87827	targeted	Present	Sample	Mgig_Bot2012_COI	T12-04	COI	45	BB87827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.81287
BB87828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88174	WGS84	BB87828	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87828_Cmae_Bot2011_COI	T12-05	1	BB87828_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87828	Sample	BB87828	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-05	COI	45	BB87828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-05	ATGAACAGTCTATCCTCCTTTAG	147.34223
BB87830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87348	WGS84	BB87830	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87830_Cjap_Sim2018_COI	T12-07	1	BB87830_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87830	Sample	BB87830	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-07	COI	45	BB87830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33661
BB87830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87348	WGS84	BB87830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87830_Mgig_Bot2012_COI	T12-07	1	BB87830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87830	Sample	BB87830	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-07	COI	45	BB87830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.33661
BB87831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82866	WGS84	BB87831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87831_Cmae_Bot2011_COI	T12-08	1	BB87831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87831	Sample	BB87831	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-08	COI	45	BB87831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-08	ATGAACAGTCTATCCTCCTTTAG	147.30169
BB87833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82518	WGS84	BB87833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87833_Aamu_Bax2006_COI	T12-10	1	BB87833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87833	Sample	BB87833	targeted	Absent	Sample	Aamu_Bax2006_COI	T12-10	COI	45	BB87833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-10	GCACAACCGGGATCTTTACTTC	147.30494
BB87833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82518	WGS84	BB87833	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87833_Pcan_Bot2011_IGS	T12-10	1	BB87833_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87833	Sample	BB87833	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-10	IGS	45	BB87833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-10	CGTAATCCTCAGTACTGGCTA	147.30494
BB87833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82518	WGS84	BB87833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87833_Sspa_Oph2007_COI	T12-10	1	BB87833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87833	Sample	BB87833	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-10	COI	45	BB87833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-10	GAAGGCCTTCCGATGATC	147.30494
BB87833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82518	WGS84	BB87833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87833_Upin_Bot2011_COI	T12-10	1	BB87833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87833	Sample	BB87833	targeted	Absent	Sample	Upin_Bot2011_COI	T12-10	COI	45	BB87833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.30494
BB87834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88553	WGS84	BB87834	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87834_Cjap_Sim2018_COI	T12-11	1	BB87834_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87834	Sample	BB87834	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-11	COI	45	BB87834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33569
BB87834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88553	WGS84	BB87834	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.751	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87834_Mgig_Bot2012_COI	T12-11	1	BB87834_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87834	Sample	BB87834	targeted	Present	Sample	Mgig_Bot2012_COI	T12-11	COI	45	BB87834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33569
BB87835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88464	WGS84	BB87835	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87835_Cmae_Bot2011_COI	T12-12	1	BB87835_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87835	Sample	BB87835	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-12	COI	45	BB87835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-12	ATGAACAGTCTATCCTCCTTTAG	147.33707
BB87837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83653	WGS84	BB87837	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87837_Aamu_Bax2006_COI	S8-25	1	BB87837_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87837	Sample	BB87837	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-25	COI	45	BB87837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-25	GCACAACCGGGATCTTTACTTC	138.50737
BB87837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83653	WGS84	BB87837	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87837_Pcan_Bot2011_IGS	S8-25	1	BB87837_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87837	Sample	BB87837	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-25	IGS	45	BB87837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-25	CGTAATCCTCAGTACTGGCTA	138.50737
BB87837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83653	WGS84	BB87837	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.135	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87837_Sspa_Oph2007_COI	S8-25	1	BB87837_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87837	Sample	BB87837	targeted	Present	Sample	Sspa_Oph2007_COI	S8-25	COI	45	BB87837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-25	GAAGGCCTTCCGATGATC	138.50737
BB87837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83653	WGS84	BB87837	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87837_Upin_Bot2011_COI	S8-25	1	BB87837_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87837	Sample	BB87837	targeted	Absent	Sample	Upin_Bot2011_COI	S8-25	COI	45	BB87837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-25	CTTTAATTACAGCGTTTTTATTGTTGT	138.50737
BB87839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83645	WGS84	BB87839	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87839_Cmae_Bot2011_COI	S8-24	1	BB87839_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87839	Sample	BB87839	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-24	COI	45	BB87839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-24	ATGAACAGTCTATCCTCCTTTAG	138.50846
BB87840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96205	WGS84	BB87840	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.511	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87840_Aamu_Bax2006_COI	T13-01	1	BB87840_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87840	Sample	BB87840	targeted	Present	Sample	Aamu_Bax2006_COI	T13-01	COI	45	BB87840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-01	GCACAACCGGGATCTTTACTTC	147.53986
BB87840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96205	WGS84	BB87840	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87840_Pcan_Bot2011_IGS	T13-01	1	BB87840_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87840	Sample	BB87840	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-01	IGS	45	BB87840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-01	CGTAATCCTCAGTACTGGCTA	147.53986
BB87840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96205	WGS84	BB87840	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87840_Sspa_Oph2007_COI	T13-01	1	BB87840_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87840	Sample	BB87840	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-01	COI	45	BB87840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-01	GAAGGCCTTCCGATGATC	147.53986
BB87840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96205	WGS84	BB87840	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87840_Upin_Bot2011_COI	T13-01	1	BB87840_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87840	Sample	BB87840	targeted	Absent	Sample	Upin_Bot2011_COI	T13-01	COI	45	BB87840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.53986
BB87841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96032	WGS84	BB87841	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87841_Cjap_Sim2018_COI	T13-02	1	BB87841_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87841	Sample	BB87841	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-02	COI	45	BB87841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53078
BB87841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96032	WGS84	BB87841	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.128	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87841_Mgig_Bot2012_COI	T13-02	1	BB87841_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87841	Sample	BB87841	targeted	Present	Sample	Mgig_Bot2012_COI	T13-02	COI	45	BB87841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.53078
BB87842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96236	WGS84	BB87842	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87842_Cmae_Bot2011_COI	T13-03	1	BB87842_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87842	Sample	BB87842	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-03	COI	45	BB87842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-03	ATGAACAGTCTATCCTCCTTTAG	147.51954
BB87844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85093	WGS84	BB87844	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87844_Aamu_Bax2006_COI	T13-05	1	BB87844_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87844	Sample	BB87844	targeted	Absent	Sample	Aamu_Bax2006_COI	T13-05	COI	45	BB87844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-05	GCACAACCGGGATCTTTACTTC	147.87402
BB87844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85093	WGS84	BB87844	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87844_Pcan_Bot2011_IGS	T13-05	1	BB87844_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87844	Sample	BB87844	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-05	IGS	45	BB87844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-05	CGTAATCCTCAGTACTGGCTA	147.87402
BB87844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85093	WGS84	BB87844	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87844_Sspa_Oph2007_COI	T13-05	1	BB87844_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87844	Sample	BB87844	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-05	COI	45	BB87844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-05	GAAGGCCTTCCGATGATC	147.87402
BB87844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85093	WGS84	BB87844	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87844_Upin_Bot2011_COI	T13-05	1	BB87844_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87844	Sample	BB87844	targeted	Absent	Sample	Upin_Bot2011_COI	T13-05	COI	45	BB87844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.87402
BB87845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85552	WGS84	BB87845	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87845_Cjap_Sim2018_COI	T13-06	1	BB87845_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87845	Sample	BB87845	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-06	COI	45	BB87845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87256
BB87845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85552	WGS84	BB87845	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87845_Mgig_Bot2012_COI	T13-06	1	BB87845_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87845	Sample	BB87845	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-06	COI	45	BB87845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.87256
BB87846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88938	WGS84	BB87846	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87846_Cmae_Bot2011_COI	T13-07	1	BB87846_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87846	Sample	BB87846	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-07	COI	45	BB87846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-07	ATGAACAGTCTATCCTCCTTTAG	147.81479
BB87848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82819	WGS84	BB87848	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87848_Aamu_Bax2006_COI	T13-09	1	BB87848_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87848	Sample	BB87848	targeted	Absent	Sample	Aamu_Bax2006_COI	T13-09	COI	45	BB87848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-09	GCACAACCGGGATCTTTACTTC	147.30194
BB87848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82819	WGS84	BB87848	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87848_Pcan_Bot2011_IGS	T13-09	1	BB87848_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87848	Sample	BB87848	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-09	IGS	45	BB87848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-09	CGTAATCCTCAGTACTGGCTA	147.30194
BB87848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82819	WGS84	BB87848	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87848_Sspa_Oph2007_COI	T13-09	1	BB87848_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87848	Sample	BB87848	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-09	COI	45	BB87848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-09	GAAGGCCTTCCGATGATC	147.30194
BB87848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82819	WGS84	BB87848	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87848_Upin_Bot2011_COI	T13-09	1	BB87848_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87848	Sample	BB87848	targeted	Absent	Sample	Upin_Bot2011_COI	T13-09	COI	45	BB87848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.30194
BB87849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82561	WGS84	BB87849	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87849_Cjap_Sim2018_COI	T13-10	1	BB87849_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87849	Sample	BB87849	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-10	COI	45	BB87849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30381
BB87849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82561	WGS84	BB87849	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87849_Mgig_Bot2012_COI	T13-10	1	BB87849_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87849	Sample	BB87849	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-10	COI	45	BB87849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.30381
BB87851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88126	WGS84	BB87851	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.779	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87851_Aamu_Bax2006_COI	T13-12	1	BB87851_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87851	Sample	BB87851	targeted	Present	Sample	Aamu_Bax2006_COI	T13-12	COI	45	BB87851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-12	GCACAACCGGGATCTTTACTTC	147.34203
BB87851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88126	WGS84	BB87851	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87851_Pcan_Bot2011_IGS	T13-12	1	BB87851_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87851	Sample	BB87851	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-12	IGS	45	BB87851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-12	CGTAATCCTCAGTACTGGCTA	147.34203
BB87851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88126	WGS84	BB87851	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87851_Sspa_Oph2007_COI	T13-12	1	BB87851_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87851	Sample	BB87851	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-12	COI	45	BB87851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-12	GAAGGCCTTCCGATGATC	147.34203
BB87851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88126	WGS84	BB87851	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87851_Upin_Bot2011_COI	T13-12	1	BB87851_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87851	Sample	BB87851	targeted	Absent	Sample	Upin_Bot2011_COI	T13-12	COI	45	BB87851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.34203
BB87852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87988	WGS84	BB87852	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87852_Cjap_Sim2018_COI	T13-13	1	BB87852_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87852	Sample	BB87852	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-13	COI	45	BB87852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3422
BB87852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87988	WGS84	BB87852	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87852_Mgig_Bot2012_COI	T13-13	1	BB87852_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87852	Sample	BB87852	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-13	COI	45	BB87852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.3422
BB87853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.86907	WGS84	BB87853	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87853_Cmae_Bot2011_COI	T13-14	1	BB87853_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87853	Sample	BB87853	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-14	COI	45	BB87853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-14	ATGAACAGTCTATCCTCCTTTAG	147.33633
BB87855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88312	WGS84	BB87855	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.289	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87855_Aamu_Bax2006_COI	T13-16	1	BB87855_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87855	Sample	BB87855	targeted	Present	Sample	Aamu_Bax2006_COI	T13-16	COI	45	BB87855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-16	GCACAACCGGGATCTTTACTTC	147.33675
BB87855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88312	WGS84	BB87855	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87855_Pcan_Bot2011_IGS	T13-16	1	BB87855_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87855	Sample	BB87855	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-16	IGS	45	BB87855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-16	CGTAATCCTCAGTACTGGCTA	147.33675
BB87855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88312	WGS84	BB87855	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87855_Sspa_Oph2007_COI	T13-16	1	BB87855_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87855	Sample	BB87855	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-16	COI	45	BB87855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-16	GAAGGCCTTCCGATGATC	147.33675
BB87855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88312	WGS84	BB87855	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87855_Upin_Bot2011_COI	T13-16	1	BB87855_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87855	Sample	BB87855	targeted	Absent	Sample	Upin_Bot2011_COI	T13-16	COI	45	BB87855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.33675
BB87856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84432	WGS84	BB87856	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87856_Cjap_Sim2018_COI	S8-16	1	BB87856_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87856	Sample	BB87856	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-16	COI	45	BB87856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49778
BB87856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84432	WGS84	BB87856	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.607	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87856_Mgig_Bot2012_COI	S8-16	1	BB87856_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87856	Sample	BB87856	targeted	Present	Sample	Mgig_Bot2012_COI	S8-16	COI	45	BB87856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.49778
BB87859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77317	WGS84	BB87859	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87859_Aamu_Bax2006_COI	S8-13	1	BB87859_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87859	Sample	BB87859	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-13	COI	45	BB87859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-13	GCACAACCGGGATCTTTACTTC	138.48449
BB87859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77317	WGS84	BB87859	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87859_Pcan_Bot2011_IGS	S8-13	1	BB87859_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87859	Sample	BB87859	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-13	IGS	45	BB87859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-13	CGTAATCCTCAGTACTGGCTA	138.48449
BB87859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77317	WGS84	BB87859	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.191	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87859_Sspa_Oph2007_COI	S8-13	1	BB87859_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87859	Sample	BB87859	targeted	Present	Sample	Sspa_Oph2007_COI	S8-13	COI	45	BB87859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-13	GAAGGCCTTCCGATGATC	138.48449
BB87859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77317	WGS84	BB87859	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87859_Upin_Bot2011_COI	S8-13	1	BB87859_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87859	Sample	BB87859	targeted	Absent	Sample	Upin_Bot2011_COI	S8-13	COI	45	BB87859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.48449
BB87860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77362	WGS84	BB87860	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87860_Cmae_Bot2011_COI	S8-12	1	BB87860_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87860	Sample	BB87860	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-12	COI	45	BB87860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-12	ATGAACAGTCTATCCTCCTTTAG	138.48487
BB87863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83652	WGS84	BB87863	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87863_Cjap_Sim2018_COI	S8-21	1	BB87863_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87863	Sample	BB87863	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-21	COI	45	BB87863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-21	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50853
BB87863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83652	WGS84	BB87863	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.068	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87863_Mgig_Bot2012_COI	S8-21	1	BB87863_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87863	Sample	BB87863	targeted	Present	Sample	Mgig_Bot2012_COI	S8-21	COI	45	BB87863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-21	TCTTATTCGTTGGAGACTTTATAACCCT	138.50853
BB87864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78706	WGS84	BB87864	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87864_Cmae_Bot2011_COI	S8-08	1	BB87864_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87864	Sample	BB87864	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-08	COI	45	BB87864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-08	ATGAACAGTCTATCCTCCTTTAG	138.51353
BB87866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78942	WGS84	BB87866	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87866_Aamu_Bax2006_COI	S8-06	1	BB87866_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87866	Sample	BB87866	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-06	COI	45	BB87866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-06	GCACAACCGGGATCTTTACTTC	138.51307
BB87866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78942	WGS84	BB87866	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87866_Pcan_Bot2011_IGS	S8-06	1	BB87866_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87866	Sample	BB87866	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-06	IGS	45	BB87866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-06	CGTAATCCTCAGTACTGGCTA	138.51307
BB87866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78942	WGS84	BB87866	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.316	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87866_Sspa_Oph2007_COI	S8-06	1	BB87866_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87866	Sample	BB87866	targeted	Present	Sample	Sspa_Oph2007_COI	S8-06	COI	45	BB87866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-06	GAAGGCCTTCCGATGATC	138.51307
BB87866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78942	WGS84	BB87866	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87866_Upin_Bot2011_COI	S8-06	1	BB87866_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87866	Sample	BB87866	targeted	Absent	Sample	Upin_Bot2011_COI	S8-06	COI	45	BB87866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.51307
BB87867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84712	WGS84	BB87867	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87867_Cjap_Sim2018_COI	S8-18	1	BB87867_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87867	Sample	BB87867	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-18	COI	45	BB87867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4976
BB87867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84712	WGS84	BB87867	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.105	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87867_Mgig_Bot2012_COI	S8-18	1	BB87867_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87867	Sample	BB87867	targeted	Present	Sample	Mgig_Bot2012_COI	S8-18	COI	45	BB87867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.4976
BB87868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87868	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87868_Cmae_Bot2011_COI	S8-17	1	BB87868_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87868	Sample	BB87868	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-17	COI	45	BB87868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-17	ATGAACAGTCTATCCTCCTTTAG	138.49707
BB87870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80894	WGS84	BB87870	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87870_Cjap_Sim2018_COI	S8-02	1	BB87870_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87870	Sample	BB87870	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-02	COI	45	BB87870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51889
BB87870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.80894	WGS84	BB87870	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87870_Mgig_Bot2012_COI	S8-02	1	BB87870_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87870	Sample	BB87870	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-02	COI	45	BB87870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.51889
BB87871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81008	WGS84	BB87871	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.072	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87871_Cmae_Bot2011_COI	S8-01	1	BB87871_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87871	Sample	BB87871	targeted	Present	Sample	Cmae_Bot2011_COI	S8-01	COI	45	BB87871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-01	ATGAACAGTCTATCCTCCTTTAG	138.51996
BB87875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8111	WGS84	BB87875	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87875_Cmae_Bot2011_COI	S6-22	1	BB87875_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87875	Sample	BB87875	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-22	COI	45	BB87875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-22	ATGAACAGTCTATCCTCCTTTAG	138.52346
BB87879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77353	WGS84	BB87879	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87879_Cmae_Bot2011_COI	S6-18	1	BB87879_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87879	Sample	BB87879	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-18	COI	45	BB87879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-18	ATGAACAGTCTATCCTCCTTTAG	138.48586
BB87880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77382	WGS84	BB87880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87880_Aamu_Bax2006_COI	S6-17	1	BB87880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87880	Sample	BB87880	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-17	COI	45	BB87880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-17	GCACAACCGGGATCTTTACTTC	138.48565
BB87880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77382	WGS84	BB87880	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87880_Pcan_Bot2011_IGS	S6-17	1	BB87880_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87880	Sample	BB87880	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-17	IGS	45	BB87880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-17	CGTAATCCTCAGTACTGGCTA	138.48565
BB87880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77382	WGS84	BB87880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87880_Sspa_Oph2007_COI	S6-17	1	BB87880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87880	Sample	BB87880	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-17	COI	45	BB87880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-17	GAAGGCCTTCCGATGATC	138.48565
BB87880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77382	WGS84	BB87880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87880_Upin_Bot2011_COI	S6-17	1	BB87880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87880	Sample	BB87880	targeted	Absent	Sample	Upin_Bot2011_COI	S6-17	COI	45	BB87880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.48565
BB87881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77307	WGS84	BB87881	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87881_Cjap_Sim2018_COI	S6-16	1	BB87881_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87881	Sample	BB87881	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-16	COI	45	BB87881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48439
BB87881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77307	WGS84	BB87881	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87881_Mgig_Bot2012_COI	S6-16	1	BB87881_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87881	Sample	BB87881	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-16	COI	45	BB87881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.48439
BB87884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78507	WGS84	BB87884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87884_Aamu_Bax2006_COI	S6-13	1	BB87884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87884	Sample	BB87884	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-13	COI	45	BB87884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-13	GCACAACCGGGATCTTTACTTC	138.51573
BB87884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78507	WGS84	BB87884	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87884_Pcan_Bot2011_IGS	S6-13	1	BB87884_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87884	Sample	BB87884	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-13	IGS	45	BB87884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-13	CGTAATCCTCAGTACTGGCTA	138.51573
BB87884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78507	WGS84	BB87884	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87884_Sspa_Oph2007_COI	S6-13	1	BB87884_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87884	Sample	BB87884	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-13	COI	45	BB87884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-13	GAAGGCCTTCCGATGATC	138.51573
BB87884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78507	WGS84	BB87884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87884_Upin_Bot2011_COI	S6-13	1	BB87884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87884	Sample	BB87884	targeted	Absent	Sample	Upin_Bot2011_COI	S6-13	COI	45	BB87884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.51573
BB87885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78633	WGS84	BB87885	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87885_Cjap_Sim2018_COI	S6-12	1	BB87885_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87885	Sample	BB87885	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-12	COI	45	BB87885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51551
BB87885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78633	WGS84	BB87885	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87885_Mgig_Bot2012_COI	S6-12	1	BB87885_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87885	Sample	BB87885	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-12	COI	45	BB87885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.51551
BB87886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78716	WGS84	BB87886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87886_Cmae_Bot2011_COI	S6-11	1	BB87886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87886	Sample	BB87886	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-11	COI	45	BB87886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-11	ATGAACAGTCTATCCTCCTTTAG	138.51506
BB87889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83736	WGS84	BB87889	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87889_Cjap_Sim2018_COI	S6-08	1	BB87889_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87889	Sample	BB87889	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-08	COI	45	BB87889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51038
BB87889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83736	WGS84	BB87889	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87889_Mgig_Bot2012_COI	S6-08	1	BB87889_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87889	Sample	BB87889	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-08	COI	45	BB87889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51038
BB87891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83658	WGS84	BB87891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87891_Aamu_Bax2006_COI	S6-06	1	BB87891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87891	Sample	BB87891	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-06	COI	45	BB87891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-06	GCACAACCGGGATCTTTACTTC	138.50805
BB87891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83658	WGS84	BB87891	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87891_Pcan_Bot2011_IGS	S6-06	1	BB87891_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87891	Sample	BB87891	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-06	IGS	45	BB87891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-06	CGTAATCCTCAGTACTGGCTA	138.50805
BB87891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83658	WGS84	BB87891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87891_Sspa_Oph2007_COI	S6-06	1	BB87891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87891	Sample	BB87891	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-06	COI	45	BB87891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-06	GAAGGCCTTCCGATGATC	138.50805
BB87891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83658	WGS84	BB87891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87891_Upin_Bot2011_COI	S6-06	1	BB87891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87891	Sample	BB87891	targeted	Absent	Sample	Upin_Bot2011_COI	S6-06	COI	45	BB87891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.50805
BB87895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84619	WGS84	BB87895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87895_Aamu_Bax2006_COI	S6-02	1	BB87895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87895	Sample	BB87895	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-02	COI	45	BB87895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-02	GCACAACCGGGATCTTTACTTC	138.49699
BB87895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84619	WGS84	BB87895	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87895_Pcan_Bot2011_IGS	S6-02	1	BB87895_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87895	Sample	BB87895	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-02	IGS	45	BB87895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-02	CGTAATCCTCAGTACTGGCTA	138.49699
BB87895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84619	WGS84	BB87895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87895_Sspa_Oph2007_COI	S6-02	1	BB87895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87895	Sample	BB87895	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-02	COI	45	BB87895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-02	GAAGGCCTTCCGATGATC	138.49699
BB87895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84619	WGS84	BB87895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87895_Upin_Bot2011_COI	S6-02	1	BB87895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87895	Sample	BB87895	targeted	Absent	Sample	Upin_Bot2011_COI	S6-02	COI	45	BB87895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.49699
BB87896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87896	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87896_Cjap_Sim2018_COI	S6-01	1	BB87896_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87896	Sample	BB87896	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-01	COI	45	BB87896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49739
BB87896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84698	WGS84	BB87896	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87896_Mgig_Bot2012_COI	S6-01	1	BB87896_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87896	Sample	BB87896	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-01	COI	45	BB87896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.49739
BB87897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85592	WGS84	BB87897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87897_Cmae_Bot2011_COI	T10-16	1	BB87897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87897	Sample	BB87897	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-16	COI	45	BB87897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-16	ATGAACAGTCTATCCTCCTTTAG	147.87241
BB87899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84692	WGS84	BB87899	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87899_Aamu_Bax2006_COI	T10-14	1	BB87899_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87899	Sample	BB87899	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-14	COI	45	BB87899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-14	GCACAACCGGGATCTTTACTTC	147.87696
BB87899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84692	WGS84	BB87899	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87899_Pcan_Bot2011_IGS	T10-14	1	BB87899_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87899	Sample	BB87899	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-14	IGS	45	BB87899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-14	CGTAATCCTCAGTACTGGCTA	147.87696
BB87899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84692	WGS84	BB87899	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87899_Sspa_Oph2007_COI	T10-14	1	BB87899_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87899	Sample	BB87899	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-14	COI	45	BB87899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-14	GAAGGCCTTCCGATGATC	147.87696
BB87899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84692	WGS84	BB87899	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87899_Upin_Bot2011_COI	T10-14	1	BB87899_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87899	Sample	BB87899	targeted	Absent	Sample	Upin_Bot2011_COI	T10-14	COI	45	BB87899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.87696
BB87900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88961	WGS84	BB87900	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87900_Cjap_Sim2018_COI	T10-13	1	BB87900_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87900	Sample	BB87900	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-13	COI	45	BB87900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81247
BB87900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88961	WGS84	BB87900	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.542	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	29/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87900_Mgig_Bot2012_COI	T10-13	1	BB87900_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87900	Sample	BB87900	targeted	Present	Sample	Mgig_Bot2012_COI	T10-13	COI	45	BB87900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.81247
BB87901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88273	WGS84	BB87901	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87901_Cmae_Bot2011_COI	T10-12	1	BB87901_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87901	Sample	BB87901	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-12	COI	45	BB87901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-12	ATGAACAGTCTATCCTCCTTTAG	147.33619
BB87903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87903_Aamu_Bax2006_COI	T10-10	1	BB87903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87903	Sample	BB87903	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-10	COI	45	BB87903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-10	GCACAACCGGGATCTTTACTTC	147.33542
BB87903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87903	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87903_Pcan_Bot2011_IGS	T10-10	1	BB87903_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87903	Sample	BB87903	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-10	IGS	45	BB87903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-10	CGTAATCCTCAGTACTGGCTA	147.33542
BB87903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87903_Sspa_Oph2007_COI	T10-10	1	BB87903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87903	Sample	BB87903	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-10	COI	45	BB87903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-10	GAAGGCCTTCCGATGATC	147.33542
BB87903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87903_Upin_Bot2011_COI	T10-10	1	BB87903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87903	Sample	BB87903	targeted	Absent	Sample	Upin_Bot2011_COI	T10-10	COI	45	BB87903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.33542
BB87904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87881	WGS84	BB87904	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87904_Cjap_Sim2018_COI	T10-09	1	BB87904_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87904	Sample	BB87904	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-09	COI	45	BB87904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34167
BB87904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87881	WGS84	BB87904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.868	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87904_Mgig_Bot2012_COI	T10-09	1	BB87904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87904	Sample	BB87904	targeted	Present	Sample	Mgig_Bot2012_COI	T10-09	COI	45	BB87904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.34167
BB87905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88078	WGS84	BB87905	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87905_Cmae_Bot2011_COI	T10-08	1	BB87905_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87905	Sample	BB87905	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-08	COI	45	BB87905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-08	ATGAACAGTCTATCCTCCTTTAG	147.34172
BB87907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82895	WGS84	BB87907	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87907_Aamu_Bax2006_COI	T10-06	1	BB87907_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87907	Sample	BB87907	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-06	COI	45	BB87907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-06	GCACAACCGGGATCTTTACTTC	147.30205
BB87907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82895	WGS84	BB87907	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87907_Pcan_Bot2011_IGS	T10-06	1	BB87907_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87907	Sample	BB87907	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-06	IGS	45	BB87907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-06	CGTAATCCTCAGTACTGGCTA	147.30205
BB87907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82895	WGS84	BB87907	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87907_Sspa_Oph2007_COI	T10-06	1	BB87907_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87907	Sample	BB87907	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-06	COI	45	BB87907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-06	GAAGGCCTTCCGATGATC	147.30205
BB87907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82895	WGS84	BB87907	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87907_Upin_Bot2011_COI	T10-06	1	BB87907_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87907	Sample	BB87907	targeted	Absent	Sample	Upin_Bot2011_COI	T10-06	COI	45	BB87907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.30205
BB87908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82753	WGS84	BB87908	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87908_Cjap_Sim2018_COI	T10-05	1	BB87908_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87908	Sample	BB87908	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-05	COI	45	BB87908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30173
BB87908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82753	WGS84	BB87908	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.365	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87908_Mgig_Bot2012_COI	T10-05	1	BB87908_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87908	Sample	BB87908	targeted	Present	Sample	Mgig_Bot2012_COI	T10-05	COI	45	BB87908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.30173
BB87909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82538	WGS84	BB87909	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.349	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87909_Cmae_Bot2011_COI	T10-04	1	BB87909_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87909	Sample	BB87909	targeted	Present	Sample	Cmae_Bot2011_COI	T10-04	COI	45	BB87909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-04	ATGAACAGTCTATCCTCCTTTAG	147.30734
BB87910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96186	WGS84	BB87910	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87910_Aamu_Bax2006_COI	T10-03	1	BB87910_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87910	Sample	BB87910	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-03	COI	45	BB87910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-03	GCACAACCGGGATCTTTACTTC	147.51957
BB87910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96186	WGS84	BB87910	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87910_Pcan_Bot2011_IGS	T10-03	1	BB87910_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87910	Sample	BB87910	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-03	IGS	45	BB87910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-03	CGTAATCCTCAGTACTGGCTA	147.51957
BB87910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96186	WGS84	BB87910	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87910_Sspa_Oph2007_COI	T10-03	1	BB87910_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87910	Sample	BB87910	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-03	COI	45	BB87910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-03	GAAGGCCTTCCGATGATC	147.51957
BB87910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96186	WGS84	BB87910	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87910_Upin_Bot2011_COI	T10-03	1	BB87910_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87910	Sample	BB87910	targeted	Absent	Sample	Upin_Bot2011_COI	T10-03	COI	45	BB87910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.51957
BB87911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96019	WGS84	BB87911	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87911_Cjap_Sim2018_COI	T10-02	1	BB87911_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87911	Sample	BB87911	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-02	COI	45	BB87911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53113
BB87911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96019	WGS84	BB87911	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.505	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87911_Mgig_Bot2012_COI	T10-02	1	BB87911_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87911	Sample	BB87911	targeted	Present	Sample	Mgig_Bot2012_COI	T10-02	COI	45	BB87911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.53113
BB87912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96295	WGS84	BB87912	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87912_Cmae_Bot2011_COI	T10-01	1	BB87912_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87912	Sample	BB87912	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-01	COI	45	BB87912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-01	ATGAACAGTCTATCCTCCTTTAG	147.53907
BB87915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81003	WGS84	BB87915	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87915_Cjap_Sim2018_COI	S5-22	1	BB87915_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87915	Sample	BB87915	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-22	COI	45	BB87915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-22	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52013
BB87915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.81003	WGS84	BB87915	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.315	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87915_Mgig_Bot2012_COI	S5-22	1	BB87915_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87915	Sample	BB87915	targeted	Present	Sample	Mgig_Bot2012_COI	S5-22	COI	45	BB87915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-22	TCTTATTCGTTGGAGACTTTATAACCCT	138.52013
BB87916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81005	WGS84	BB87916	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87916_Cmae_Bot2011_COI	S5-21	1	BB87916_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87916	Sample	BB87916	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-21	COI	45	BB87916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-21	ATGAACAGTCTATCCTCCTTTAG	138.51897
BB87919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77349	WGS84	BB87919	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87919_Cjap_Sim2018_COI	S5-18	1	BB87919_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87919	Sample	BB87919	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-18	COI	45	BB87919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48577
BB87919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77349	WGS84	BB87919	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87919_Mgig_Bot2012_COI	S5-18	1	BB87919_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87919	Sample	BB87919	targeted	Absent	Sample	Mgig_Bot2012_COI	S5-18	COI	45	BB87919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.48577
BB87921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77137	WGS84	BB87921	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87921_Aamu_Bax2006_COI	S5-16	1	BB87921_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87921	Sample	BB87921	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-16	COI	45	BB87921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-16	GCACAACCGGGATCTTTACTTC	138.48612
BB87921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77137	WGS84	BB87921	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87921_Pcan_Bot2011_IGS	S5-16	1	BB87921_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87921	Sample	BB87921	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-16	IGS	45	BB87921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-16	CGTAATCCTCAGTACTGGCTA	138.48612
BB87921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77137	WGS84	BB87921	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.15	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87921_Sspa_Oph2007_COI	S5-16	1	BB87921_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87921	Sample	BB87921	targeted	Present	Sample	Sspa_Oph2007_COI	S5-16	COI	45	BB87921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-16	GAAGGCCTTCCGATGATC	138.48612
BB87921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77137	WGS84	BB87921	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87921_Upin_Bot2011_COI	S5-16	1	BB87921_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87921	Sample	BB87921	targeted	Absent	Sample	Upin_Bot2011_COI	S5-16	COI	45	BB87921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.48612
BB87925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78656	WGS84	BB87925	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87925_Aamu_Bax2006_COI	S5-12	1	BB87925_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87925	Sample	BB87925	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-12	COI	45	BB87925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-12	GCACAACCGGGATCTTTACTTC	138.51548
BB87925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78656	WGS84	BB87925	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87925_Pcan_Bot2011_IGS	S5-12	1	BB87925_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87925	Sample	BB87925	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-12	IGS	45	BB87925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-12	CGTAATCCTCAGTACTGGCTA	138.51548
BB87925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78656	WGS84	BB87925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.185	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87925_Sspa_Oph2007_COI	S5-12	1	BB87925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87925	Sample	BB87925	targeted	Present	Sample	Sspa_Oph2007_COI	S5-12	COI	45	BB87925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-12	GAAGGCCTTCCGATGATC	138.51548
BB87925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78656	WGS84	BB87925	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87925_Upin_Bot2011_COI	S5-12	1	BB87925_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87925	Sample	BB87925	targeted	Absent	Sample	Upin_Bot2011_COI	S5-12	COI	45	BB87925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.51548
BB87926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78764	WGS84	BB87926	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87926_Cjap_Sim2018_COI	S5-11	1	BB87926_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87926	Sample	BB87926	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-11	COI	45	BB87926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51513
BB87926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78764	WGS84	BB87926	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.708	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87926_Mgig_Bot2012_COI	S5-11	1	BB87926_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87926	Sample	BB87926	targeted	Present	Sample	Mgig_Bot2012_COI	S5-11	COI	45	BB87926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51513
BB87929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83656	WGS84	BB87929	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87929_Aamu_Bax2006_COI	S5-08	1	BB87929_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87929	Sample	BB87929	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-08	COI	45	BB87929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-08	GCACAACCGGGATCTTTACTTC	138.50972
BB87929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83656	WGS84	BB87929	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87929_Pcan_Bot2011_IGS	S5-08	1	BB87929_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87929	Sample	BB87929	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-08	IGS	45	BB87929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-08	CGTAATCCTCAGTACTGGCTA	138.50972
BB87929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83656	WGS84	BB87929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.371	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87929_Sspa_Oph2007_COI	S5-08	1	BB87929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87929	Sample	BB87929	targeted	Present	Sample	Sspa_Oph2007_COI	S5-08	COI	45	BB87929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-08	GAAGGCCTTCCGATGATC	138.50972
BB87929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83656	WGS84	BB87929	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87929_Upin_Bot2011_COI	S5-08	1	BB87929_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87929	Sample	BB87929	targeted	Absent	Sample	Upin_Bot2011_COI	S5-08	COI	45	BB87929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.50972
BB87930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83622	WGS84	BB87930	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87930_Cmae_Bot2011_COI	S5-07	1	BB87930_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87930	Sample	BB87930	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-07	COI	45	BB87930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-07	ATGAACAGTCTATCCTCCTTTAG	138.50894
BB87934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8452	WGS84	BB87934	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87934_Cmae_Bot2011_COI	S5-03	1	BB87934_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87934	Sample	BB87934	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-03	COI	45	BB87934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-03	ATGAACAGTCTATCCTCCTTTAG	138.49715
BB87936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87936	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87936_Aamu_Bax2006_COI	S5-01	1	BB87936_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87936	Sample	BB87936	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-01	COI	45	BB87936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-01	GCACAACCGGGATCTTTACTTC	138.49736
BB87936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87936	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87936_Pcan_Bot2011_IGS	S5-01	1	BB87936_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87936	Sample	BB87936	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-01	IGS	45	BB87936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-01	CGTAATCCTCAGTACTGGCTA	138.49736
BB87936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87936	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.514	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87936_Sspa_Oph2007_COI	S5-01	1	BB87936_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87936	Sample	BB87936	targeted	Present	Sample	Sspa_Oph2007_COI	S5-01	COI	45	BB87936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-01	GAAGGCCTTCCGATGATC	138.49736
BB87936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87936	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87936_Upin_Bot2011_COI	S5-01	1	BB87936_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87936	Sample	BB87936	targeted	Absent	Sample	Upin_Bot2011_COI	S5-01	COI	45	BB87936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.49736
BB87937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88278	WGS84	BB87937	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87937_Cjap_Sim2018_COI	T9-16	1	BB87937_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87937	Sample	BB87937	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-16	COI	45	BB87937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33629
BB87937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88278	WGS84	BB87937	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.231	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87937_Mgig_Bot2012_COI	T9-16	1	BB87937_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87937	Sample	BB87937	targeted	Present	Sample	Mgig_Bot2012_COI	T9-16	COI	45	BB87937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.33629
BB87938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88434	WGS84	BB87938	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87938_Cmae_Bot2011_COI	T9-15	1	BB87938_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87938	Sample	BB87938	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-15	COI	45	BB87938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-15	ATGAACAGTCTATCCTCCTTTAG	147.33624
BB87940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87919	WGS84	BB87940	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87940_Cjap_Sim2018_COI	T9-13	1	BB87940_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87940	Sample	BB87940	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-13	COI	45	BB87940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3417
BB87940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.87919	WGS84	BB87940	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.684	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87940_Mgig_Bot2012_COI	T9-13	1	BB87940_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87940	Sample	BB87940	targeted	Present	Sample	Mgig_Bot2012_COI	T9-13	COI	45	BB87940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.3417
BB87941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8813	WGS84	BB87941	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87941_Cmae_Bot2011_COI	T9-12	1	BB87941_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87941	Sample	BB87941	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-12	COI	45	BB87941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-12	ATGAACAGTCTATCCTCCTTTAG	147.3416
BB87943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82517	WGS84	BB87943	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87943_Aamu_Bax2006_COI	T9-10	1	BB87943_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87943	Sample	BB87943	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-10	COI	45	BB87943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-10	GCACAACCGGGATCTTTACTTC	147.30705
BB87943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82517	WGS84	BB87943	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87943_Pcan_Bot2011_IGS	T9-10	1	BB87943_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87943	Sample	BB87943	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-10	IGS	45	BB87943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-10	CGTAATCCTCAGTACTGGCTA	147.30705
BB87943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82517	WGS84	BB87943	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87943_Sspa_Oph2007_COI	T9-10	1	BB87943_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87943	Sample	BB87943	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-10	COI	45	BB87943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-10	GAAGGCCTTCCGATGATC	147.30705
BB87943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82517	WGS84	BB87943	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87943_Upin_Bot2011_COI	T9-10	1	BB87943_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87943	Sample	BB87943	targeted	Absent	Sample	Upin_Bot2011_COI	T9-10	COI	45	BB87943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.30705
BB87944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82698	WGS84	BB87944	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87944_Cjap_Sim2018_COI	T9-09	1	BB87944_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87944	Sample	BB87944	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-09	COI	45	BB87944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30243
BB87944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82698	WGS84	BB87944	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.188	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87944_Mgig_Bot2012_COI	T9-09	1	BB87944_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87944	Sample	BB87944	targeted	Present	Sample	Mgig_Bot2012_COI	T9-09	COI	45	BB87944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.30243
BB87945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8292	WGS84	BB87945	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87945_Cmae_Bot2011_COI	T9-08	1	BB87945_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87945	Sample	BB87945	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-08	COI	45	BB87945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-08	ATGAACAGTCTATCCTCCTTTAG	147.30183
BB87947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85078	WGS84	BB87947	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87947_Aamu_Bax2006_COI	T9-06	1	BB87947_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87947	Sample	BB87947	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-06	COI	45	BB87947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-06	GCACAACCGGGATCTTTACTTC	147.87365
BB87947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85078	WGS84	BB87947	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87947_Pcan_Bot2011_IGS	T9-06	1	BB87947_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87947	Sample	BB87947	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-06	IGS	45	BB87947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-06	CGTAATCCTCAGTACTGGCTA	147.87365
BB87947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85078	WGS84	BB87947	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87947_Sspa_Oph2007_COI	T9-06	1	BB87947_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87947	Sample	BB87947	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-06	COI	45	BB87947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-06	GAAGGCCTTCCGATGATC	147.87365
BB87947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85078	WGS84	BB87947	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87947_Upin_Bot2011_COI	T9-06	1	BB87947_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87947	Sample	BB87947	targeted	Absent	Sample	Upin_Bot2011_COI	T9-06	COI	45	BB87947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.87365
BB87948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84699	WGS84	BB87948	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87948_Cjap_Sim2018_COI	T9-05	1	BB87948_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87948	Sample	BB87948	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-05	COI	45	BB87948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87517
BB87948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.84699	WGS84	BB87948	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.16	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	23/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87948_Mgig_Bot2012_COI	T9-05	1	BB87948_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87948	Sample	BB87948	targeted	Present	Sample	Mgig_Bot2012_COI	T9-05	COI	45	BB87948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.87517
BB87949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88957	WGS84	BB87949	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.681	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87949_Cmae_Bot2011_COI	T9-04	1	BB87949_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87949	Sample	BB87949	targeted	Present	Sample	Cmae_Bot2011_COI	T9-04	COI	45	BB87949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-04	ATGAACAGTCTATCCTCCTTTAG	147.81295
BB87950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96213	WGS84	BB87950	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87950_Aamu_Bax2006_COI	T9-03	1	BB87950_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87950	Sample	BB87950	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-03	COI	45	BB87950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-03	GCACAACCGGGATCTTTACTTC	147.51947
BB87950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96213	WGS84	BB87950	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87950_Pcan_Bot2011_IGS	T9-03	1	BB87950_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87950	Sample	BB87950	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-03	IGS	45	BB87950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-03	CGTAATCCTCAGTACTGGCTA	147.51947
BB87950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96213	WGS84	BB87950	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87950_Sspa_Oph2007_COI	T9-03	1	BB87950_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87950	Sample	BB87950	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-03	COI	45	BB87950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-03	GAAGGCCTTCCGATGATC	147.51947
BB87950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96213	WGS84	BB87950	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87950_Upin_Bot2011_COI	T9-03	1	BB87950_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87950	Sample	BB87950	targeted	Absent	Sample	Upin_Bot2011_COI	T9-03	COI	45	BB87950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.51947
BB87951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96033	WGS84	BB87951	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87951_Cjap_Sim2018_COI	T9-02	1	BB87951_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87951	Sample	BB87951	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-02	COI	45	BB87951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53072
BB87951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96033	WGS84	BB87951	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.291	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	22/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87951_Mgig_Bot2012_COI	T9-02	1	BB87951_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87951	Sample	BB87951	targeted	Present	Sample	Mgig_Bot2012_COI	T9-02	COI	45	BB87951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.53072
BB87952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96322	WGS84	BB87952	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87952_Cmae_Bot2011_COI	T9-01	1	BB87952_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87952	Sample	BB87952	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-01	COI	45	BB87952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-01	ATGAACAGTCTATCCTCCTTTAG	147.5383
BB87955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80982	WGS84	BB87955	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87955_Cjap_Sim2018_COI	S4-18	1	BB87955_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87955	Sample	BB87955	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-18	COI	45	BB87955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51977
BB87955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.80982	WGS84	BB87955	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.233	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87955_Mgig_Bot2012_COI	S4-18	1	BB87955_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87955	Sample	BB87955	targeted	Present	Sample	Mgig_Bot2012_COI	S4-18	COI	45	BB87955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.51977
BB87956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80946	WGS84	BB87956	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87956_Cmae_Bot2011_COI	S4-17	1	BB87956_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87956	Sample	BB87956	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-17	COI	45	BB87956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-17	ATGAACAGTCTATCCTCCTTTAG	138.51847
BB87959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83615	WGS84	BB87959	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.714	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87959_Mgig_Bot2012_COI	S4-15	1	BB87959_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87959	Sample	BB87959	targeted	Present	Sample	Mgig_Bot2012_COI	S4-15	COI	45	BB87959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.5083
BB93410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18844	WGS84	BB93410	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93410_Aamu_Bax2006_COI	DD4	1	BB93410_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93410	Sample	BB93410	targeted	Absent	Sample	Aamu_Bax2006_COI	DD4	COI	45	BB93410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD4	GCACAACCGGGATCTTTACTTC	146.36786
BB93410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18844	WGS84	BB93410	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93410_Pcan_Bot2011_IGS	DD4	1	BB93410_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93410	Sample	BB93410	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD4	IGS	45	BB93410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD4	CGTAATCCTCAGTACTGGCTA	146.36786
BB93410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18844	WGS84	BB93410	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93410_Sspa_Oph2007_COI	DD4	1	BB93410_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93410	Sample	BB93410	targeted	Absent	Sample	Sspa_Oph2007_COI	DD4	COI	45	BB93410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD4	GAAGGCCTTCCGATGATC	146.36786
BB93410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18844	WGS84	BB93410	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93410_Upin_Bot2011_COI	DD4	1	BB93410_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93410	Sample	BB93410	targeted	Absent	Sample	Upin_Bot2011_COI	DD4	COI	45	BB93410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD4	CTTTAATTACAGCGTTTTTATTGTTGT	146.36786
BB93412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85169	WGS84	BB93412	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93412_Cmae_Bot2011_COI	GD95	1	BB93412_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93412	Sample	BB93412	targeted	Absent	Sample	Cmae_Bot2011_COI	GD95	COI	45	BB93412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD95	ATGAACAGTCTATCCTCCTTTAG	151.31021
BB93412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85169	WGS84	BB93412	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93412_Msal_Bot2012_COI	GD95	1	BB93412_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93412	Sample	BB93412	targeted	Absent	Sample	Msal_Bot2012_COI	GD95	COI	45	BB93412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD95	GYTAGTTCCRATGATGTTAGCTG	151.31021
BB93414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7897	WGS84	BB93414	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93414_Aamu_Bax2006_COI	GD80	1	BB93414_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93414	Sample	BB93414	targeted	Absent	Sample	Aamu_Bax2006_COI	GD80	COI	45	BB93414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD80	GCACAACCGGGATCTTTACTTC	151.20943
BB93414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7897	WGS84	BB93414	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93414_Pcan_Bot2011_IGS	GD80	1	BB93414_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93414	Sample	BB93414	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD80	IGS	45	BB93414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD80	CGTAATCCTCAGTACTGGCTA	151.20943
BB93414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7897	WGS84	BB93414	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93414_Sspa_Oph2007_COI	GD80	1	BB93414_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93414	Sample	BB93414	targeted	Absent	Sample	Sspa_Oph2007_COI	GD80	COI	45	BB93414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD80	GAAGGCCTTCCGATGATC	151.20943
BB93414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7897	WGS84	BB93414	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93414_Upin_Bot2011_COI	GD80	1	BB93414_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93414	Sample	BB93414	targeted	Absent	Sample	Upin_Bot2011_COI	GD80	COI	45	BB93414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD80	CTTTAATTACAGCGTTTTTATTGTTGT	151.20943
BB93415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78606	WGS84	BB93415	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93415_Mgig_Bot2012_COI	GD70	1	BB93415_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93415	Sample	BB93415	targeted	Absent	Sample	Mgig_Bot2012_COI	GD70	COI	45	BB93415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD70	TCTTATTCGTTGGAGACTTTATAACCCT	151.17164
BB93416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78758	WGS84	BB93416	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93416_Cmae_Bot2011_COI	GD65	1	BB93416_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93416	Sample	BB93416	targeted	Absent	Sample	Cmae_Bot2011_COI	GD65	COI	45	BB93416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD65	ATGAACAGTCTATCCTCCTTTAG	151.17433
BB93416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78758	WGS84	BB93416	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93416_Msal_Bot2012_COI	GD65	1	BB93416_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93416	Sample	BB93416	targeted	Absent	Sample	Msal_Bot2012_COI	GD65	COI	45	BB93416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD65	GYTAGTTCCRATGATGTTAGCTG	151.17433
BB93418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80527	WGS84	BB93418	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93418_Aamu_Bax2006_COI	GD50	1	BB93418_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93418	Sample	BB93418	targeted	Absent	Sample	Aamu_Bax2006_COI	GD50	COI	45	BB93418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD50	GCACAACCGGGATCTTTACTTC	151.21539
BB93418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80527	WGS84	BB93418	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93418_Pcan_Bot2011_IGS	GD50	1	BB93418_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93418	Sample	BB93418	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD50	IGS	45	BB93418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD50	CGTAATCCTCAGTACTGGCTA	151.21539
BB93418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80527	WGS84	BB93418	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93418_Sspa_Oph2007_COI	GD50	1	BB93418_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93418	Sample	BB93418	targeted	Absent	Sample	Sspa_Oph2007_COI	GD50	COI	45	BB93418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD50	GAAGGCCTTCCGATGATC	151.21539
BB93418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80527	WGS84	BB93418	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93418_Upin_Bot2011_COI	GD50	1	BB93418_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93418	Sample	BB93418	targeted	Absent	Sample	Upin_Bot2011_COI	GD50	COI	45	BB93418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD50	CTTTAATTACAGCGTTTTTATTGTTGT	151.21539
BB93419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81656	WGS84	BB93419	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93419_Mgig_Bot2012_COI	GD107	1	BB93419_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93419	Sample	BB93419	targeted	Absent	Sample	Mgig_Bot2012_COI	GD107	COI	45	BB93419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD107	TCTTATTCGTTGGAGACTTTATAACCCT	151.24038
BB93421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83117	WGS84	BB93421	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93421_Aamu_Bax2006_COI	GD34	1	BB93421_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93421	Sample	BB93421	targeted	Absent	Sample	Aamu_Bax2006_COI	GD34	COI	45	BB93421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD34	GCACAACCGGGATCTTTACTTC	151.25505
BB93421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83117	WGS84	BB93421	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93421_Pcan_Bot2011_IGS	GD34	1	BB93421_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93421	Sample	BB93421	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD34	IGS	45	BB93421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD34	CGTAATCCTCAGTACTGGCTA	151.25505
BB93421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83117	WGS84	BB93421	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93421_Sspa_Oph2007_COI	GD34	1	BB93421_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93421	Sample	BB93421	targeted	Absent	Sample	Sspa_Oph2007_COI	GD34	COI	45	BB93421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD34	GAAGGCCTTCCGATGATC	151.25505
BB93421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83117	WGS84	BB93421	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93421_Upin_Bot2011_COI	GD34	1	BB93421_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93421	Sample	BB93421	targeted	Absent	Sample	Upin_Bot2011_COI	GD34	COI	45	BB93421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD34	CTTTAATTACAGCGTTTTTATTGTTGT	151.25505
BB93422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83171	WGS84	BB93422	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93422_Mgig_Bot2012_COI	GD36	1	BB93422_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93422	Sample	BB93422	targeted	Absent	Sample	Mgig_Bot2012_COI	GD36	COI	45	BB93422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD36	TCTTATTCGTTGGAGACTTTATAACCCT	151.25897
BB93423	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83198	WGS84	BB93423	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93423_Cmae_Bot2011_COI	GD37	1	BB93423_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93423	Sample	BB93423	targeted	Absent	Sample	Cmae_Bot2011_COI	GD37	COI	45	BB93423	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD37	ATGAACAGTCTATCCTCCTTTAG	151.26071
BB93423	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83198	WGS84	BB93423	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93423_Msal_Bot2012_COI	GD37	1	BB93423_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93423	Sample	BB93423	targeted	Absent	Sample	Msal_Bot2012_COI	GD37	COI	45	BB93423	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD37	GYTAGTTCCRATGATGTTAGCTG	151.26071
BB93425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83282	WGS84	BB93425	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93425_Aamu_Bax2006_COI	GD42	1	BB93425_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93425	Sample	BB93425	targeted	Absent	Sample	Aamu_Bax2006_COI	GD42	COI	45	BB93425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD42	GCACAACCGGGATCTTTACTTC	151.26352
BB93425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83282	WGS84	BB93425	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93425_Pcan_Bot2011_IGS	GD42	1	BB93425_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93425	Sample	BB93425	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD42	IGS	45	BB93425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD42	CGTAATCCTCAGTACTGGCTA	151.26352
BB93425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83282	WGS84	BB93425	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93425_Sspa_Oph2007_COI	GD42	1	BB93425_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93425	Sample	BB93425	targeted	Absent	Sample	Sspa_Oph2007_COI	GD42	COI	45	BB93425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD42	GAAGGCCTTCCGATGATC	151.26352
BB93425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83282	WGS84	BB93425	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93425_Upin_Bot2011_COI	GD42	1	BB93425_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93425	Sample	BB93425	targeted	Absent	Sample	Upin_Bot2011_COI	GD42	COI	45	BB93425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD42	CTTTAATTACAGCGTTTTTATTGTTGT	151.26352
BB93426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8062	WGS84	BB93426	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93426_Mgig_Bot2012_COI	GD43	1	BB93426_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93426	Sample	BB93426	targeted	Absent	Sample	Mgig_Bot2012_COI	GD43	COI	45	BB93426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD43	TCTTATTCGTTGGAGACTTTATAACCCT	151.22027
BB93427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83562	WGS84	BB93427	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93427_Cmae_Bot2011_COI	GD101	1	BB93427_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93427	Sample	BB93427	targeted	Absent	Sample	Cmae_Bot2011_COI	GD101	COI	45	BB93427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD101	ATGAACAGTCTATCCTCCTTTAG	151.27317
BB93427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83562	WGS84	BB93427	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93427_Msal_Bot2012_COI	GD101	1	BB93427_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93427	Sample	BB93427	targeted	Absent	Sample	Msal_Bot2012_COI	GD101	COI	45	BB93427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD101	GYTAGTTCCRATGATGTTAGCTG	151.27317
BB93429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80704	WGS84	BB93429	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93429_Aamu_Bax2006_COI	GD47	1	BB93429_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93429	Sample	BB93429	targeted	Absent	Sample	Aamu_Bax2006_COI	GD47	COI	45	BB93429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD47	GCACAACCGGGATCTTTACTTC	151.21656
BB93429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80704	WGS84	BB93429	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93429_Pcan_Bot2011_IGS	GD47	1	BB93429_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93429	Sample	BB93429	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD47	IGS	45	BB93429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD47	CGTAATCCTCAGTACTGGCTA	151.21656
BB93429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80704	WGS84	BB93429	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93429_Sspa_Oph2007_COI	GD47	1	BB93429_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93429	Sample	BB93429	targeted	Absent	Sample	Sspa_Oph2007_COI	GD47	COI	45	BB93429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD47	GAAGGCCTTCCGATGATC	151.21656
BB93429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80704	WGS84	BB93429	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93429_Upin_Bot2011_COI	GD47	1	BB93429_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93429	Sample	BB93429	targeted	Absent	Sample	Upin_Bot2011_COI	GD47	COI	45	BB93429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD47	CTTTAATTACAGCGTTTTTATTGTTGT	151.21656
BB93430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80758	WGS84	BB93430	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93430_Cmae_Bot2011_COI	GD48	1	BB93430_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93430	Sample	BB93430	targeted	Absent	Sample	Cmae_Bot2011_COI	GD48	COI	45	BB93430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD48	ATGAACAGTCTATCCTCCTTTAG	151.21655
BB93430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80758	WGS84	BB93430	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93430_Msal_Bot2012_COI	GD48	1	BB93430_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93430	Sample	BB93430	targeted	Absent	Sample	Msal_Bot2012_COI	GD48	COI	45	BB93430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD48	GYTAGTTCCRATGATGTTAGCTG	151.21655
BB93432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79141	WGS84	BB93432	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93432_Aamu_Bax2006_COI	GD54	1	BB93432_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93432	Sample	BB93432	targeted	Absent	Sample	Aamu_Bax2006_COI	GD54	COI	45	BB93432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD54	GCACAACCGGGATCTTTACTTC	151.17851
BB93432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79141	WGS84	BB93432	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93432_Pcan_Bot2011_IGS	GD54	1	BB93432_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93432	Sample	BB93432	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD54	IGS	45	BB93432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD54	CGTAATCCTCAGTACTGGCTA	151.17851
BB93432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79141	WGS84	BB93432	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93432_Sspa_Oph2007_COI	GD54	1	BB93432_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93432	Sample	BB93432	targeted	Absent	Sample	Sspa_Oph2007_COI	GD54	COI	45	BB93432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD54	GAAGGCCTTCCGATGATC	151.17851
BB93432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79141	WGS84	BB93432	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93432_Upin_Bot2011_COI	GD54	1	BB93432_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93432	Sample	BB93432	targeted	Absent	Sample	Upin_Bot2011_COI	GD54	COI	45	BB93432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD54	CTTTAATTACAGCGTTTTTATTGTTGT	151.17851
BB93433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79121	WGS84	BB93433	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93433_Mgig_Bot2012_COI	GD56	1	BB93433_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93433	Sample	BB93433	targeted	Absent	Sample	Mgig_Bot2012_COI	GD56	COI	45	BB93433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD56	TCTTATTCGTTGGAGACTTTATAACCCT	151.18219
BB93434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78964	WGS84	BB93434	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93434_Cmae_Bot2011_COI	GD58	1	BB93434_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93434	Sample	BB93434	targeted	Absent	Sample	Cmae_Bot2011_COI	GD58	COI	45	BB93434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD58	ATGAACAGTCTATCCTCCTTTAG	151.1796
BB93434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78964	WGS84	BB93434	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93434_Msal_Bot2012_COI	GD58	1	BB93434_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93434	Sample	BB93434	targeted	Absent	Sample	Msal_Bot2012_COI	GD58	COI	45	BB93434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD58	GYTAGTTCCRATGATGTTAGCTG	151.1796
BB93436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78713	WGS84	BB93436	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93436_Aamu_Bax2006_COI	GD63	1	BB93436_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93436	Sample	BB93436	targeted	Absent	Sample	Aamu_Bax2006_COI	GD63	COI	45	BB93436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD63	GCACAACCGGGATCTTTACTTC	151.17623
BB93436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78713	WGS84	BB93436	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93436_Pcan_Bot2011_IGS	GD63	1	BB93436_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93436	Sample	BB93436	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD63	IGS	45	BB93436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD63	CGTAATCCTCAGTACTGGCTA	151.17623
BB93436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78713	WGS84	BB93436	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93436_Sspa_Oph2007_COI	GD63	1	BB93436_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93436	Sample	BB93436	targeted	Absent	Sample	Sspa_Oph2007_COI	GD63	COI	45	BB93436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD63	GAAGGCCTTCCGATGATC	151.17623
BB93436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78713	WGS84	BB93436	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93436_Upin_Bot2011_COI	GD63	1	BB93436_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93436	Sample	BB93436	targeted	Absent	Sample	Upin_Bot2011_COI	GD63	COI	45	BB93436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD63	CTTTAATTACAGCGTTTTTATTGTTGT	151.17623
BB93437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78794	WGS84	BB93437	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93437_Mgig_Bot2012_COI	GD66	1	BB93437_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93437	Sample	BB93437	targeted	Absent	Sample	Mgig_Bot2012_COI	GD66	COI	45	BB93437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD66	TCTTATTCGTTGGAGACTTTATAACCCT	151.17475
BB93438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78494	WGS84	BB93438	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93438_Cmae_Bot2011_COI	GD68	1	BB93438_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93438	Sample	BB93438	targeted	Absent	Sample	Cmae_Bot2011_COI	GD68	COI	45	BB93438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD68	ATGAACAGTCTATCCTCCTTTAG	151.17338
BB93438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78494	WGS84	BB93438	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93438_Msal_Bot2012_COI	GD68	1	BB93438_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93438	Sample	BB93438	targeted	Absent	Sample	Msal_Bot2012_COI	GD68	COI	45	BB93438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD68	GYTAGTTCCRATGATGTTAGCTG	151.17338
BB93440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7776	WGS84	BB93440	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93440_Mgig_Bot2012_COI	GD72	1	BB93440_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93440	Sample	BB93440	targeted	Absent	Sample	Mgig_Bot2012_COI	GD72	COI	45	BB93440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD72	TCTTATTCGTTGGAGACTTTATAACCCT	151.19514
BB93441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.77933	WGS84	BB93441	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93441_Cmae_Bot2011_COI	GD74	1	BB93441_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93441	Sample	BB93441	targeted	Absent	Sample	Cmae_Bot2011_COI	GD74	COI	45	BB93441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD74	ATGAACAGTCTATCCTCCTTTAG	151.19654
BB93441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.77933	WGS84	BB93441	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93441_Msal_Bot2012_COI	GD74	1	BB93441_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93441	Sample	BB93441	targeted	Absent	Sample	Msal_Bot2012_COI	GD74	COI	45	BB93441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD74	GYTAGTTCCRATGATGTTAGCTG	151.19654
BB93443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78389	WGS84	BB93443	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93443_Aamu_Bax2006_COI	GD78	1	BB93443_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93443	Sample	BB93443	targeted	Absent	Sample	Aamu_Bax2006_COI	GD78	COI	45	BB93443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD78	GCACAACCGGGATCTTTACTTC	151.20134
BB93443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78389	WGS84	BB93443	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93443_Pcan_Bot2011_IGS	GD78	1	BB93443_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93443	Sample	BB93443	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD78	IGS	45	BB93443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD78	CGTAATCCTCAGTACTGGCTA	151.20134
BB93443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78389	WGS84	BB93443	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93443_Sspa_Oph2007_COI	GD78	1	BB93443_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93443	Sample	BB93443	targeted	Absent	Sample	Sspa_Oph2007_COI	GD78	COI	45	BB93443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD78	GAAGGCCTTCCGATGATC	151.20134
BB93443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78389	WGS84	BB93443	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93443_Upin_Bot2011_COI	GD78	1	BB93443_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93443	Sample	BB93443	targeted	Absent	Sample	Upin_Bot2011_COI	GD78	COI	45	BB93443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD78	CTTTAATTACAGCGTTTTTATTGTTGT	151.20134
BB93444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78485	WGS84	BB93444	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93444_Mgig_Bot2012_COI	GD79	1	BB93444_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93444	Sample	BB93444	targeted	Absent	Sample	Mgig_Bot2012_COI	GD79	COI	45	BB93444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD79	TCTTATTCGTTGGAGACTTTATAACCCT	151.20342
BB93445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79192	WGS84	BB93445	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93445_Cmae_Bot2011_COI	GD82	1	BB93445_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93445	Sample	BB93445	targeted	Absent	Sample	Cmae_Bot2011_COI	GD82	COI	45	BB93445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD82	ATGAACAGTCTATCCTCCTTTAG	151.21144
BB93445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79192	WGS84	BB93445	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93445_Msal_Bot2012_COI	GD82	1	BB93445_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93445	Sample	BB93445	targeted	Absent	Sample	Msal_Bot2012_COI	GD82	COI	45	BB93445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD82	GYTAGTTCCRATGATGTTAGCTG	151.21144
BB93447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85551	WGS84	BB93447	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93447_Aamu_Bax2006_COI	GD86	1	BB93447_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93447	Sample	BB93447	targeted	Absent	Sample	Aamu_Bax2006_COI	GD86	COI	45	BB93447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD86	GCACAACCGGGATCTTTACTTC	151.32168
BB93447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85551	WGS84	BB93447	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93447_Pcan_Bot2011_IGS	GD86	1	BB93447_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93447	Sample	BB93447	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD86	IGS	45	BB93447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD86	CGTAATCCTCAGTACTGGCTA	151.32168
BB93447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85551	WGS84	BB93447	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93447_Sspa_Oph2007_COI	GD86	1	BB93447_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93447	Sample	BB93447	targeted	Absent	Sample	Sspa_Oph2007_COI	GD86	COI	45	BB93447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD86	GAAGGCCTTCCGATGATC	151.32168
BB93447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85551	WGS84	BB93447	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93447_Upin_Bot2011_COI	GD86	1	BB93447_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93447	Sample	BB93447	targeted	Absent	Sample	Upin_Bot2011_COI	GD86	COI	45	BB93447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD86	CTTTAATTACAGCGTTTTTATTGTTGT	151.32168
BB93448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85314	WGS84	BB93448	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93448_Mgig_Bot2012_COI	GD88	1	BB93448_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93448	Sample	BB93448	targeted	Absent	Sample	Mgig_Bot2012_COI	GD88	COI	45	BB93448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD88	TCTTATTCGTTGGAGACTTTATAACCCT	151.31966
BB93449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8526	WGS84	BB93449	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93449_Cmae_Bot2011_COI	GD91	1	BB93449_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93449	Sample	BB93449	targeted	Absent	Sample	Cmae_Bot2011_COI	GD91	COI	45	BB93449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD91	ATGAACAGTCTATCCTCCTTTAG	151.31603
BB93449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8526	WGS84	BB93449	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93449_Msal_Bot2012_COI	GD91	1	BB93449_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93449	Sample	BB93449	targeted	Absent	Sample	Msal_Bot2012_COI	GD91	COI	45	BB93449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD91	GYTAGTTCCRATGATGTTAGCTG	151.31603
BB93450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85126	WGS84	BB93450	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93450_Aamu_Bax2006_COI	GD93	1	BB93450_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93450	Sample	BB93450	targeted	Absent	Sample	Aamu_Bax2006_COI	GD93	COI	45	BB93450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD93	GCACAACCGGGATCTTTACTTC	151.31393
BB93450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85126	WGS84	BB93450	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93450_Pcan_Bot2011_IGS	GD93	1	BB93450_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93450	Sample	BB93450	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD93	IGS	45	BB93450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD93	CGTAATCCTCAGTACTGGCTA	151.31393
BB93450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85126	WGS84	BB93450	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93450_Sspa_Oph2007_COI	GD93	1	BB93450_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93450	Sample	BB93450	targeted	Absent	Sample	Sspa_Oph2007_COI	GD93	COI	45	BB93450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD93	GAAGGCCTTCCGATGATC	151.31393
BB93450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85126	WGS84	BB93450	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93450_Upin_Bot2011_COI	GD93	1	BB93450_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93450	Sample	BB93450	targeted	Absent	Sample	Upin_Bot2011_COI	GD93	COI	45	BB93450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD93	CTTTAATTACAGCGTTTTTATTGTTGT	151.31393
BB93451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85305	WGS84	BB93451	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93451_Mgig_Bot2012_COI	GD97	1	BB93451_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93451	Sample	BB93451	targeted	Absent	Sample	Mgig_Bot2012_COI	GD97	COI	45	BB93451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD97	TCTTATTCGTTGGAGACTTTATAACCCT	151.3086
BB93452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81936	WGS84	BB93452	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93452_Cmae_Bot2011_COI	GD10	1	BB93452_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93452	Sample	BB93452	targeted	Absent	Sample	Cmae_Bot2011_COI	GD10	COI	45	BB93452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD10	ATGAACAGTCTATCCTCCTTTAG	151.24067
BB93452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81936	WGS84	BB93452	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93452_Msal_Bot2012_COI	GD10	1	BB93452_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93452	Sample	BB93452	targeted	Absent	Sample	Msal_Bot2012_COI	GD10	COI	45	BB93452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD10	GYTAGTTCCRATGATGTTAGCTG	151.24067
BB93454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82217	WGS84	BB93454	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93454_Aamu_Bax2006_COI	GD20	1	BB93454_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93454	Sample	BB93454	targeted	Absent	Sample	Aamu_Bax2006_COI	GD20	COI	45	BB93454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD20	GCACAACCGGGATCTTTACTTC	151.24371
BB93454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82217	WGS84	BB93454	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93454_Pcan_Bot2011_IGS	GD20	1	BB93454_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93454	Sample	BB93454	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD20	IGS	45	BB93454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD20	CGTAATCCTCAGTACTGGCTA	151.24371
BB93454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82217	WGS84	BB93454	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93454_Sspa_Oph2007_COI	GD20	1	BB93454_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93454	Sample	BB93454	targeted	Absent	Sample	Sspa_Oph2007_COI	GD20	COI	45	BB93454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD20	GAAGGCCTTCCGATGATC	151.24371
BB93454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82217	WGS84	BB93454	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93454_Upin_Bot2011_COI	GD20	1	BB93454_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93454	Sample	BB93454	targeted	Absent	Sample	Upin_Bot2011_COI	GD20	COI	45	BB93454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD20	CTTTAATTACAGCGTTTTTATTGTTGT	151.24371
BB93455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82269	WGS84	BB93455	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93455_Mgig_Bot2012_COI	GD25	1	BB93455_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93455	Sample	BB93455	targeted	Absent	Sample	Mgig_Bot2012_COI	GD25	COI	45	BB93455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD25	TCTTATTCGTTGGAGACTTTATAACCCT	151.24141
BB93456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82442	WGS84	BB93456	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93456_Cmae_Bot2011_COI	GD30	1	BB93456_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93456	Sample	BB93456	targeted	Absent	Sample	Cmae_Bot2011_COI	GD30	COI	45	BB93456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD30	ATGAACAGTCTATCCTCCTTTAG	151.24865
BB93456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82442	WGS84	BB93456	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93456_Msal_Bot2012_COI	GD30	1	BB93456_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93456	Sample	BB93456	targeted	Absent	Sample	Msal_Bot2012_COI	GD30	COI	45	BB93456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD30	GYTAGTTCCRATGATGTTAGCTG	151.24865
BB93458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82097	WGS84	BB93458	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93458_Aamu_Bax2006_COI	GD110	1	BB93458_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93458	Sample	BB93458	targeted	Absent	Sample	Aamu_Bax2006_COI	GD110	COI	45	BB93458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD110	GCACAACCGGGATCTTTACTTC	151.24484
BB93458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82097	WGS84	BB93458	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93458_Pcan_Bot2011_IGS	GD110	1	BB93458_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93458	Sample	BB93458	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD110	IGS	45	BB93458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD110	CGTAATCCTCAGTACTGGCTA	151.24484
BB93458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82097	WGS84	BB93458	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93458_Sspa_Oph2007_COI	GD110	1	BB93458_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93458	Sample	BB93458	targeted	Absent	Sample	Sspa_Oph2007_COI	GD110	COI	45	BB93458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD110	GAAGGCCTTCCGATGATC	151.24484
BB93458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82097	WGS84	BB93458	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93458_Upin_Bot2011_COI	GD110	1	BB93458_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93458	Sample	BB93458	targeted	Absent	Sample	Upin_Bot2011_COI	GD110	COI	45	BB93458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD110	CTTTAATTACAGCGTTTTTATTGTTGT	151.24484
BB93459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83496	WGS84	BB93459	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93459_Mgig_Bot2012_COI	GD40	1	BB93459_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93459	Sample	BB93459	targeted	Absent	Sample	Mgig_Bot2012_COI	GD40	COI	45	BB93459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD40	TCTTATTCGTTGGAGACTTTATAACCCT	151.26835
BB93461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80734	WGS84	BB93461	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93461_Aamu_Bax2006_COI	GD45	1	BB93461_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93461	Sample	BB93461	targeted	Absent	Sample	Aamu_Bax2006_COI	GD45	COI	45	BB93461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD45	GCACAACCGGGATCTTTACTTC	151.21889
BB93461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80734	WGS84	BB93461	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93461_Pcan_Bot2011_IGS	GD45	1	BB93461_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93461	Sample	BB93461	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD45	IGS	45	BB93461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD45	CGTAATCCTCAGTACTGGCTA	151.21889
BB93461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80734	WGS84	BB93461	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93461_Sspa_Oph2007_COI	GD45	1	BB93461_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93461	Sample	BB93461	targeted	Absent	Sample	Sspa_Oph2007_COI	GD45	COI	45	BB93461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD45	GAAGGCCTTCCGATGATC	151.21889
BB93461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80734	WGS84	BB93461	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93461_Upin_Bot2011_COI	GD45	1	BB93461_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93461	Sample	BB93461	targeted	Absent	Sample	Upin_Bot2011_COI	GD45	COI	45	BB93461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD45	CTTTAATTACAGCGTTTTTATTGTTGT	151.21889
BB93462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82706	WGS84	BB93462	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93462_Mgig_Bot2012_COI	GD1	1	BB93462_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93462	Sample	BB93462	targeted	Absent	Sample	Mgig_Bot2012_COI	GD1	COI	45	BB93462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD1	TCTTATTCGTTGGAGACTTTATAACCCT	151.24741
BB93463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82693	WGS84	BB93463	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93463_Cmae_Bot2011_COI	GD2	1	BB93463_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93463	Sample	BB93463	targeted	Absent	Sample	Cmae_Bot2011_COI	GD2	COI	45	BB93463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD2	ATGAACAGTCTATCCTCCTTTAG	151.24805
BB93463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82693	WGS84	BB93463	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93463_Msal_Bot2012_COI	GD2	1	BB93463_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93463	Sample	BB93463	targeted	Absent	Sample	Msal_Bot2012_COI	GD2	COI	45	BB93463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD2	GYTAGTTCCRATGATGTTAGCTG	151.24805
BB93465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81546	WGS84	BB93465	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93465_Aamu_Bax2006_COI	GD4	1	BB93465_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93465	Sample	BB93465	targeted	Absent	Sample	Aamu_Bax2006_COI	GD4	COI	45	BB93465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD4	GCACAACCGGGATCTTTACTTC	151.23717
BB93465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81546	WGS84	BB93465	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93465_Pcan_Bot2011_IGS	GD4	1	BB93465_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93465	Sample	BB93465	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD4	IGS	45	BB93465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD4	CGTAATCCTCAGTACTGGCTA	151.23717
BB93465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81546	WGS84	BB93465	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93465_Sspa_Oph2007_COI	GD4	1	BB93465_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93465	Sample	BB93465	targeted	Absent	Sample	Sspa_Oph2007_COI	GD4	COI	45	BB93465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD4	GAAGGCCTTCCGATGATC	151.23717
BB93465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81546	WGS84	BB93465	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93465_Upin_Bot2011_COI	GD4	1	BB93465_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93465	Sample	BB93465	targeted	Absent	Sample	Upin_Bot2011_COI	GD4	COI	45	BB93465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD4	CTTTAATTACAGCGTTTTTATTGTTGT	151.23717
BB93466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8178	WGS84	BB93466	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93466_Mgig_Bot2012_COI	GD7	1	BB93466_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93466	Sample	BB93466	targeted	Absent	Sample	Mgig_Bot2012_COI	GD7	COI	45	BB93466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD7	TCTTATTCGTTGGAGACTTTATAACCCT	151.23909
BB93467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81887	WGS84	BB93467	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93467_Cmae_Bot2011_COI	GD9	1	BB93467_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93467	Sample	BB93467	targeted	Absent	Sample	Cmae_Bot2011_COI	GD9	COI	45	BB93467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD9	ATGAACAGTCTATCCTCCTTTAG	151.23964
BB93467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81887	WGS84	BB93467	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93467_Msal_Bot2012_COI	GD9	1	BB93467_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93467	Sample	BB93467	targeted	Absent	Sample	Msal_Bot2012_COI	GD9	COI	45	BB93467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD9	GYTAGTTCCRATGATGTTAGCTG	151.23964
BB93469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82166	WGS84	BB93469	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93469_Aamu_Bax2006_COI	GD14	1	BB93469_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93469	Sample	BB93469	targeted	Absent	Sample	Aamu_Bax2006_COI	GD14	COI	45	BB93469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD14	GCACAACCGGGATCTTTACTTC	151.24024
BB93469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82166	WGS84	BB93469	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93469_Pcan_Bot2011_IGS	GD14	1	BB93469_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93469	Sample	BB93469	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD14	IGS	45	BB93469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD14	CGTAATCCTCAGTACTGGCTA	151.24024
BB93469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82166	WGS84	BB93469	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93469_Sspa_Oph2007_COI	GD14	1	BB93469_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93469	Sample	BB93469	targeted	Absent	Sample	Sspa_Oph2007_COI	GD14	COI	45	BB93469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD14	GAAGGCCTTCCGATGATC	151.24024
BB93469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82166	WGS84	BB93469	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93469_Upin_Bot2011_COI	GD14	1	BB93469_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93469	Sample	BB93469	targeted	Absent	Sample	Upin_Bot2011_COI	GD14	COI	45	BB93469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD14	CTTTAATTACAGCGTTTTTATTGTTGT	151.24024
BB93470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81992	WGS84	BB93470	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93470_Cmae_Bot2011_COI	GD17	1	BB93470_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93470	Sample	BB93470	targeted	Absent	Sample	Cmae_Bot2011_COI	GD17	COI	45	BB93470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD17	ATGAACAGTCTATCCTCCTTTAG	151.24107
BB93470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81992	WGS84	BB93470	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93470_Msal_Bot2012_COI	GD17	1	BB93470_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93470	Sample	BB93470	targeted	Absent	Sample	Msal_Bot2012_COI	GD17	COI	45	BB93470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD17	GYTAGTTCCRATGATGTTAGCTG	151.24107
BB93472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82256	WGS84	BB93472	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93472_Aamu_Bax2006_COI	GD21	1	BB93472_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93472	Sample	BB93472	targeted	Absent	Sample	Aamu_Bax2006_COI	GD21	COI	45	BB93472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD21	GCACAACCGGGATCTTTACTTC	151.24253
BB93472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82256	WGS84	BB93472	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93472_Pcan_Bot2011_IGS	GD21	1	BB93472_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93472	Sample	BB93472	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD21	IGS	45	BB93472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD21	CGTAATCCTCAGTACTGGCTA	151.24253
BB93472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82256	WGS84	BB93472	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93472_Sspa_Oph2007_COI	GD21	1	BB93472_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93472	Sample	BB93472	targeted	Absent	Sample	Sspa_Oph2007_COI	GD21	COI	45	BB93472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD21	GAAGGCCTTCCGATGATC	151.24253
BB93472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82256	WGS84	BB93472	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93472_Upin_Bot2011_COI	GD21	1	BB93472_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93472	Sample	BB93472	targeted	Absent	Sample	Upin_Bot2011_COI	GD21	COI	45	BB93472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD21	CTTTAATTACAGCGTTTTTATTGTTGT	151.24253
BB93473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82382	WGS84	BB93473	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93473_Mgig_Bot2012_COI	GD27	1	BB93473_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93473	Sample	BB93473	targeted	Absent	Sample	Mgig_Bot2012_COI	GD27	COI	45	BB93473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD27	TCTTATTCGTTGGAGACTTTATAACCCT	151.24513
BB93474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82492	WGS84	BB93474	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93474_Cmae_Bot2011_COI	GD28	1	BB93474_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93474	Sample	BB93474	targeted	Absent	Sample	Cmae_Bot2011_COI	GD28	COI	45	BB93474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD28	ATGAACAGTCTATCCTCCTTTAG	151.24519
BB93474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82492	WGS84	BB93474	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93474_Msal_Bot2012_COI	GD28	1	BB93474_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93474	Sample	BB93474	targeted	Absent	Sample	Msal_Bot2012_COI	GD28	COI	45	BB93474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD28	GYTAGTTCCRATGATGTTAGCTG	151.24519
BB93476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82527	WGS84	BB93476	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93476_Aamu_Bax2006_COI	GD33	1	BB93476_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93476	Sample	BB93476	targeted	Absent	Sample	Aamu_Bax2006_COI	GD33	COI	45	BB93476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD33	GCACAACCGGGATCTTTACTTC	151.24526
BB93476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82527	WGS84	BB93476	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93476_Pcan_Bot2011_IGS	GD33	1	BB93476_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93476	Sample	BB93476	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD33	IGS	45	BB93476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD33	CGTAATCCTCAGTACTGGCTA	151.24526
BB93476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82527	WGS84	BB93476	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93476_Sspa_Oph2007_COI	GD33	1	BB93476_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93476	Sample	BB93476	targeted	Absent	Sample	Sspa_Oph2007_COI	GD33	COI	45	BB93476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD33	GAAGGCCTTCCGATGATC	151.24526
BB93476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82527	WGS84	BB93476	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93476_Upin_Bot2011_COI	GD33	1	BB93476_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93476	Sample	BB93476	targeted	Absent	Sample	Upin_Bot2011_COI	GD33	COI	45	BB93476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD33	CTTTAATTACAGCGTTTTTATTGTTGT	151.24526
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93477_Mgig_Bot2012_COI	MD125	1	BB93477_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Absent	Sample	Mgig_Bot2012_COI	MD125	COI	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	TCTTATTCGTTGGAGACTTTATAACCCT	144.90157
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93478_Cmae_Bot2011_COI	MD120	1	BB93478_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Absent	Sample	Cmae_Bot2011_COI	MD120	COI	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	ATGAACAGTCTATCCTCCTTTAG	144.90171
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93478_Hsan_Wil2021_COI	MD120	1	BB93478_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Absent	Sample	Hsan_Wil2021_COI	MD120	COI	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	CCTGGGCCGGTATAGTAGGT	144.90171
BB93478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82517	WGS84	BB93478	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93478_Msal_Bot2012_COI	MD120	1	BB93478_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93478	Sample	BB93478	targeted	Absent	Sample	Msal_Bot2012_COI	MD120	COI	45	BB93478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD120	GYTAGTTCCRATGATGTTAGCTG	144.90171
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93480_Mgig_Bot2012_COI	MD105	1	BB93480_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Absent	Sample	Mgig_Bot2012_COI	MD105	COI	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	TCTTATTCGTTGGAGACTTTATAACCCT	144.91047
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.614	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93481_Cmae_Bot2011_COI	MD100	1	BB93481_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Present	Sample	Cmae_Bot2011_COI	MD100	COI	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	ATGAACAGTCTATCCTCCTTTAG	144.91348
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93481_Hsan_Wil2021_COI	MD100	1	BB93481_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Absent	Sample	Hsan_Wil2021_COI	MD100	COI	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	CCTGGGCCGGTATAGTAGGT	144.91348
BB93481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81915	WGS84	BB93481	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93481_Msal_Bot2012_COI	MD100	1	BB93481_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93481	Sample	BB93481	targeted	Absent	Sample	Msal_Bot2012_COI	MD100	COI	45	BB93481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD100	GYTAGTTCCRATGATGTTAGCTG	144.91348
BB93483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81802	WGS84	BB93483	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.608	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93483_Aamu_Bax2006_COI	MD85	1	BB93483_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93483	Sample	BB93483	targeted	Present	Sample	Aamu_Bax2006_COI	MD85	COI	45	BB93483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD85	GCACAACCGGGATCTTTACTTC	144.91266
BB93483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81802	WGS84	BB93483	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93483_Pcan_Bot2011_IGS	MD85	1	BB93483_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93483	Sample	BB93483	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD85	IGS	45	BB93483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD85	CGTAATCCTCAGTACTGGCTA	144.91266
BB93483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81802	WGS84	BB93483	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.032	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93483_Sspa_Oph2007_COI	MD85	1	BB93483_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93483	Sample	BB93483	targeted	Present	Sample	Sspa_Oph2007_COI	MD85	COI	45	BB93483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD85	GAAGGCCTTCCGATGATC	144.91266
BB93483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81802	WGS84	BB93483	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.285	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93483_Upin_Bot2011_COI	MD85	1	BB93483_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93483	Sample	BB93483	targeted	Present	Sample	Upin_Bot2011_COI	MD85	COI	45	BB93483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD85	CTTTAATTACAGCGTTTTTATTGTTGT	144.91266
BB93484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81957	WGS84	BB93484	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93484_Mgig_Bot2012_COI	MD80	1	BB93484_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93484	Sample	BB93484	targeted	Absent	Sample	Mgig_Bot2012_COI	MD80	COI	45	BB93484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD80	TCTTATTCGTTGGAGACTTTATAACCCT	144.91825
BB93485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81669	WGS84	BB93485	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93485_Cmae_Bot2011_COI	MD70	1	BB93485_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93485	Sample	BB93485	targeted	Absent	Sample	Cmae_Bot2011_COI	MD70	COI	45	BB93485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD70	ATGAACAGTCTATCCTCCTTTAG	144.92406
BB93485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81669	WGS84	BB93485	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93485_Msal_Bot2012_COI	MD70	1	BB93485_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93485	Sample	BB93485	targeted	Absent	Sample	Msal_Bot2012_COI	MD70	COI	45	BB93485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD70	GYTAGTTCCRATGATGTTAGCTG	144.92406
BB93487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8189	WGS84	BB93487	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.152	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93487_Aamu_Bax2006_COI	MD60	1	BB93487_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93487	Sample	BB93487	targeted	Present	Sample	Aamu_Bax2006_COI	MD60	COI	45	BB93487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD60	GCACAACCGGGATCTTTACTTC	144.92881
BB93487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8189	WGS84	BB93487	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93487_Pcan_Bot2011_IGS	MD60	1	BB93487_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93487	Sample	BB93487	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD60	IGS	45	BB93487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD60	CGTAATCCTCAGTACTGGCTA	144.92881
BB93487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8189	WGS84	BB93487	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.998	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93487_Sspa_Oph2007_COI	MD60	1	BB93487_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93487	Sample	BB93487	targeted	Present	Sample	Sspa_Oph2007_COI	MD60	COI	45	BB93487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD60	GAAGGCCTTCCGATGATC	144.92881
BB93487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8189	WGS84	BB93487	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93487_Upin_Bot2011_COI	MD60	1	BB93487_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93487	Sample	BB93487	targeted	Absent	Sample	Upin_Bot2011_COI	MD60	COI	45	BB93487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD60	CTTTAATTACAGCGTTTTTATTGTTGT	144.92881
BB93488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86098	WGS84	BB93488	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93488_Mgig_Bot2012_COI	MD55	1	BB93488_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93488	Sample	BB93488	targeted	Absent	Sample	Mgig_Bot2012_COI	MD55	COI	45	BB93488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD55	TCTTATTCGTTGGAGACTTTATAACCCT	144.90725
BB93489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93489	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.515	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93489_Cmae_Bot2011_COI	MD45	1	BB93489_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93489	Sample	BB93489	targeted	Present	Sample	Cmae_Bot2011_COI	MD45	COI	45	BB93489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD45	ATGAACAGTCTATCCTCCTTTAG	144.91313
BB93489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93489	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93489_Msal_Bot2012_COI	MD45	1	BB93489_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93489	Sample	BB93489	targeted	Absent	Sample	Msal_Bot2012_COI	MD45	COI	45	BB93489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD45	GYTAGTTCCRATGATGTTAGCTG	144.91313
BB93490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93490	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.578	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93490_Aamu_Bax2006_COI	MD35	1	BB93490_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93490	Sample	BB93490	targeted	Present	Sample	Aamu_Bax2006_COI	MD35	COI	45	BB93490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD35	GCACAACCGGGATCTTTACTTC	144.90751
BB93490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93490	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93490_Pcan_Bot2011_IGS	MD35	1	BB93490_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93490	Sample	BB93490	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD35	IGS	45	BB93490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD35	CGTAATCCTCAGTACTGGCTA	144.90751
BB93490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93490	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.635	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93490_Sspa_Oph2007_COI	MD35	1	BB93490_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93490	Sample	BB93490	targeted	Present	Sample	Sspa_Oph2007_COI	MD35	COI	45	BB93490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD35	GAAGGCCTTCCGATGATC	144.90751
BB93490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93490	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.963	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93490_Upin_Bot2011_COI	MD35	1	BB93490_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93490	Sample	BB93490	targeted	Present	Sample	Upin_Bot2011_COI	MD35	COI	45	BB93490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD35	CTTTAATTACAGCGTTTTTATTGTTGT	144.90751
BB93491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86484	WGS84	BB93491	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93491_Mgig_Bot2012_COI	MD30	1	BB93491_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93491	Sample	BB93491	targeted	Absent	Sample	Mgig_Bot2012_COI	MD30	COI	45	BB93491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD30	TCTTATTCGTTGGAGACTTTATAACCCT	144.91759
BB93492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8467	WGS84	BB93492	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.701	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93492_Cmae_Bot2011_COI	MD20	1	BB93492_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93492	Sample	BB93492	targeted	Present	Sample	Cmae_Bot2011_COI	MD20	COI	45	BB93492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD20	ATGAACAGTCTATCCTCCTTTAG	144.90834
BB93492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8467	WGS84	BB93492	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93492_Msal_Bot2012_COI	MD20	1	BB93492_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93492	Sample	BB93492	targeted	Absent	Sample	Msal_Bot2012_COI	MD20	COI	45	BB93492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD20	GYTAGTTCCRATGATGTTAGCTG	144.90834
BB93494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82574	WGS84	BB93494	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.559	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93494_Aamu_Bax2006_COI	MD123	1	BB93494_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93494	Sample	BB93494	targeted	Present	Sample	Aamu_Bax2006_COI	MD123	COI	45	BB93494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD123	GCACAACCGGGATCTTTACTTC	144.90211
BB93494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82574	WGS84	BB93494	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93494_Pcan_Bot2011_IGS	MD123	1	BB93494_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93494	Sample	BB93494	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD123	IGS	45	BB93494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD123	CGTAATCCTCAGTACTGGCTA	144.90211
BB93494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82574	WGS84	BB93494	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.287	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93494_Sspa_Oph2007_COI	MD123	1	BB93494_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93494	Sample	BB93494	targeted	Present	Sample	Sspa_Oph2007_COI	MD123	COI	45	BB93494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD123	GAAGGCCTTCCGATGATC	144.90211
BB93494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82574	WGS84	BB93494	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93494_Upin_Bot2011_COI	MD123	1	BB93494_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93494	Sample	BB93494	targeted	Absent	Sample	Upin_Bot2011_COI	MD123	COI	45	BB93494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD123	CTTTAATTACAGCGTTTTTATTGTTGT	144.90211
BB93495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8245	WGS84	BB93495	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93495_Mgig_Bot2012_COI	MD119	1	BB93495_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93495	Sample	BB93495	targeted	Absent	Sample	Mgig_Bot2012_COI	MD119	COI	45	BB93495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD119	TCTTATTCGTTGGAGACTTTATAACCCT	144.904
BB93496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82348	WGS84	BB93496	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93496_Cmae_Bot2011_COI	MD117	1	BB93496_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93496	Sample	BB93496	targeted	Absent	Sample	Cmae_Bot2011_COI	MD117	COI	45	BB93496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD117	ATGAACAGTCTATCCTCCTTTAG	144.90236
BB93496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82348	WGS84	BB93496	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93496_Msal_Bot2012_COI	MD117	1	BB93496_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93496	Sample	BB93496	targeted	Absent	Sample	Msal_Bot2012_COI	MD117	COI	45	BB93496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD117	GYTAGTTCCRATGATGTTAGCTG	144.90236
BB93498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81641	WGS84	BB93498	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.364	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93498_Aamu_Bax2006_COI	MD111	1	BB93498_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93498	Sample	BB93498	targeted	Present	Sample	Aamu_Bax2006_COI	MD111	COI	45	BB93498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD111	GCACAACCGGGATCTTTACTTC	144.90597
BB93498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81641	WGS84	BB93498	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93498_Pcan_Bot2011_IGS	MD111	1	BB93498_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93498	Sample	BB93498	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD111	IGS	45	BB93498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD111	CGTAATCCTCAGTACTGGCTA	144.90597
BB93498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81641	WGS84	BB93498	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.988	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93498_Sspa_Oph2007_COI	MD111	1	BB93498_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93498	Sample	BB93498	targeted	Present	Sample	Sspa_Oph2007_COI	MD111	COI	45	BB93498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD111	GAAGGCCTTCCGATGATC	144.90597
BB93498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81641	WGS84	BB93498	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93498_Upin_Bot2011_COI	MD111	1	BB93498_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93498	Sample	BB93498	targeted	Absent	Sample	Upin_Bot2011_COI	MD111	COI	45	BB93498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD111	CTTTAATTACAGCGTTTTTATTGTTGT	144.90597
BB93499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82141	WGS84	BB93499	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93499_Mgig_Bot2012_COI	MD108	1	BB93499_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93499	Sample	BB93499	targeted	Absent	Sample	Mgig_Bot2012_COI	MD108	COI	45	BB93499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD108	TCTTATTCGTTGGAGACTTTATAACCCT	144.90788
BB93500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82007	WGS84	BB93500	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93500_Cmae_Bot2011_COI	MD107	1	BB93500_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93500	Sample	BB93500	targeted	Absent	Sample	Cmae_Bot2011_COI	MD107	COI	45	BB93500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD107	ATGAACAGTCTATCCTCCTTTAG	144.90792
BB93500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82007	WGS84	BB93500	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93500_Msal_Bot2012_COI	MD107	1	BB93500_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93500	Sample	BB93500	targeted	Absent	Sample	Msal_Bot2012_COI	MD107	COI	45	BB93500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD107	GYTAGTTCCRATGATGTTAGCTG	144.90792
BB93502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82112	WGS84	BB93502	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.349	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93502_Aamu_Bax2006_COI	MD104	1	BB93502_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93502	Sample	BB93502	targeted	Present	Sample	Aamu_Bax2006_COI	MD104	COI	45	BB93502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD104	GCACAACCGGGATCTTTACTTC	144.91172
BB93502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82112	WGS84	BB93502	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93502_Pcan_Bot2011_IGS	MD104	1	BB93502_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93502	Sample	BB93502	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD104	IGS	45	BB93502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD104	CGTAATCCTCAGTACTGGCTA	144.91172
BB93502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82112	WGS84	BB93502	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.604	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93502_Sspa_Oph2007_COI	MD104	1	BB93502_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93502	Sample	BB93502	targeted	Present	Sample	Sspa_Oph2007_COI	MD104	COI	45	BB93502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD104	GAAGGCCTTCCGATGATC	144.91172
BB93502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82112	WGS84	BB93502	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93502_Upin_Bot2011_COI	MD104	1	BB93502_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93502	Sample	BB93502	targeted	Absent	Sample	Upin_Bot2011_COI	MD104	COI	45	BB93502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD104	CTTTAATTACAGCGTTTTTATTGTTGT	144.91172
BB93503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8201	WGS84	BB93503	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93503_Mgig_Bot2012_COI	MD101	1	BB93503_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93503	Sample	BB93503	targeted	Absent	Sample	Mgig_Bot2012_COI	MD101	COI	45	BB93503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD101	TCTTATTCGTTGGAGACTTTATAACCCT	144.91155
BB93504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93504	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93504_Cmae_Bot2011_COI	MD98	1	BB93504_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93504	Sample	BB93504	targeted	Absent	Sample	Cmae_Bot2011_COI	MD98	COI	45	BB93504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD98	ATGAACAGTCTATCCTCCTTTAG	144.91364
BB93504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93504	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93504_Msal_Bot2012_COI	MD98	1	BB93504_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93504	Sample	BB93504	targeted	Absent	Sample	Msal_Bot2012_COI	MD98	COI	45	BB93504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD98	GYTAGTTCCRATGATGTTAGCTG	144.91364
BB93506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81156	WGS84	BB93506	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.102	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93506_Aamu_Bax2006_COI	MD94	1	BB93506_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93506	Sample	BB93506	targeted	Present	Sample	Aamu_Bax2006_COI	MD94	COI	45	BB93506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD94	GCACAACCGGGATCTTTACTTC	144.91409
BB93506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81156	WGS84	BB93506	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93506_Pcan_Bot2011_IGS	MD94	1	BB93506_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93506	Sample	BB93506	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD94	IGS	45	BB93506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD94	CGTAATCCTCAGTACTGGCTA	144.91409
BB93506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81156	WGS84	BB93506	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.621	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93506_Sspa_Oph2007_COI	MD94	1	BB93506_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93506	Sample	BB93506	targeted	Present	Sample	Sspa_Oph2007_COI	MD94	COI	45	BB93506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD94	GAAGGCCTTCCGATGATC	144.91409
BB93506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81156	WGS84	BB93506	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93506_Upin_Bot2011_COI	MD94	1	BB93506_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93506	Sample	BB93506	targeted	Absent	Sample	Upin_Bot2011_COI	MD94	COI	45	BB93506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD94	CTTTAATTACAGCGTTTTTATTGTTGT	144.91409
BB93507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81165	WGS84	BB93507	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93507_Mgig_Bot2012_COI	MD93	1	BB93507_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93507	Sample	BB93507	targeted	Absent	Sample	Mgig_Bot2012_COI	MD93	COI	45	BB93507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD93	TCTTATTCGTTGGAGACTTTATAACCCT	144.91297
BB93508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81445	WGS84	BB93508	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93508_Cmae_Bot2011_COI	MD91	1	BB93508_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93508	Sample	BB93508	targeted	Absent	Sample	Cmae_Bot2011_COI	MD91	COI	45	BB93508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD91	ATGAACAGTCTATCCTCCTTTAG	144.91369
BB93508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81445	WGS84	BB93508	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93508_Msal_Bot2012_COI	MD91	1	BB93508_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93508	Sample	BB93508	targeted	Absent	Sample	Msal_Bot2012_COI	MD91	COI	45	BB93508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD91	GYTAGTTCCRATGATGTTAGCTG	144.91369
BB93510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81717	WGS84	BB93510	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93510_Mgig_Bot2012_COI	MD86	1	BB93510_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93510	Sample	BB93510	targeted	Absent	Sample	Mgig_Bot2012_COI	MD86	COI	45	BB93510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD86	TCTTATTCGTTGGAGACTTTATAACCCT	144.91329
BB93511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81836	WGS84	BB93511	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93511_Cmae_Bot2011_COI	MD83	1	BB93511_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93511	Sample	BB93511	targeted	Absent	Sample	Cmae_Bot2011_COI	MD83	COI	45	BB93511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD83	ATGAACAGTCTATCCTCCTTTAG	144.91492
BB93511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81836	WGS84	BB93511	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93511_Msal_Bot2012_COI	MD83	1	BB93511_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93511	Sample	BB93511	targeted	Absent	Sample	Msal_Bot2012_COI	MD83	COI	45	BB93511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD83	GYTAGTTCCRATGATGTTAGCTG	144.91492
BB93513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81971	WGS84	BB93513	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.771	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93513_Aamu_Bax2006_COI	MD79	1	BB93513_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93513	Sample	BB93513	targeted	Present	Sample	Aamu_Bax2006_COI	MD79	COI	45	BB93513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD79	GCACAACCGGGATCTTTACTTC	144.91962
BB93513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81971	WGS84	BB93513	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93513_Pcan_Bot2011_IGS	MD79	1	BB93513_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93513	Sample	BB93513	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD79	IGS	45	BB93513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD79	CGTAATCCTCAGTACTGGCTA	144.91962
BB93513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81971	WGS84	BB93513	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.854	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93513_Sspa_Oph2007_COI	MD79	1	BB93513_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93513	Sample	BB93513	targeted	Present	Sample	Sspa_Oph2007_COI	MD79	COI	45	BB93513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD79	GAAGGCCTTCCGATGATC	144.91962
BB93513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81971	WGS84	BB93513	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93513_Upin_Bot2011_COI	MD79	1	BB93513_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93513	Sample	BB93513	targeted	Absent	Sample	Upin_Bot2011_COI	MD79	COI	45	BB93513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD79	CTTTAATTACAGCGTTTTTATTGTTGT	144.91962
BB93514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81923	WGS84	BB93514	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93514_Mgig_Bot2012_COI	MD77	1	BB93514_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93514	Sample	BB93514	targeted	Absent	Sample	Mgig_Bot2012_COI	MD77	COI	45	BB93514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD77	TCTTATTCGTTGGAGACTTTATAACCCT	144.92092
BB93515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81903	WGS84	BB93515	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93515_Cmae_Bot2011_COI	MD74	1	BB93515_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93515	Sample	BB93515	targeted	Absent	Sample	Cmae_Bot2011_COI	MD74	COI	45	BB93515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD74	ATGAACAGTCTATCCTCCTTTAG	144.92243
BB93515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81903	WGS84	BB93515	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93515_Msal_Bot2012_COI	MD74	1	BB93515_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93515	Sample	BB93515	targeted	Absent	Sample	Msal_Bot2012_COI	MD74	COI	45	BB93515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD74	GYTAGTTCCRATGATGTTAGCTG	144.92243
BB93517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81825	WGS84	BB93517	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.557	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93517_Aamu_Bax2006_COI	MD71	1	BB93517_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93517	Sample	BB93517	targeted	Present	Sample	Aamu_Bax2006_COI	MD71	COI	45	BB93517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD71	GCACAACCGGGATCTTTACTTC	144.92325
BB93517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81825	WGS84	BB93517	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93517_Pcan_Bot2011_IGS	MD71	1	BB93517_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93517	Sample	BB93517	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD71	IGS	45	BB93517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD71	CGTAATCCTCAGTACTGGCTA	144.92325
BB93517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81825	WGS84	BB93517	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.767	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93517_Sspa_Oph2007_COI	MD71	1	BB93517_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93517	Sample	BB93517	targeted	Present	Sample	Sspa_Oph2007_COI	MD71	COI	45	BB93517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD71	GAAGGCCTTCCGATGATC	144.92325
BB93517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81825	WGS84	BB93517	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93517_Upin_Bot2011_COI	MD71	1	BB93517_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93517	Sample	BB93517	targeted	Absent	Sample	Upin_Bot2011_COI	MD71	COI	45	BB93517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD71	CTTTAATTACAGCGTTTTTATTGTTGT	144.92325
BB93518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81672	WGS84	BB93518	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93518_Mgig_Bot2012_COI	MD68	1	BB93518_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93518	Sample	BB93518	targeted	Absent	Sample	Mgig_Bot2012_COI	MD68	COI	45	BB93518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD68	TCTTATTCGTTGGAGACTTTATAACCCT	144.92453
BB93519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8179	WGS84	BB93519	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93519_Cmae_Bot2011_COI	MD62	1	BB93519_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93519	Sample	BB93519	targeted	Absent	Sample	Cmae_Bot2011_COI	MD62	COI	45	BB93519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD62	ATGAACAGTCTATCCTCCTTTAG	144.92486
BB93519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8179	WGS84	BB93519	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93519_Msal_Bot2012_COI	MD62	1	BB93519_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93519	Sample	BB93519	targeted	Absent	Sample	Msal_Bot2012_COI	MD62	COI	45	BB93519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD62	GYTAGTTCCRATGATGTTAGCTG	144.92486
BB93520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81935	WGS84	BB93520	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.266	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93520_Aamu_Bax2006_COI	MD63	1	BB93520_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93520	Sample	BB93520	targeted	Present	Sample	Aamu_Bax2006_COI	MD63	COI	45	BB93520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD63	GCACAACCGGGATCTTTACTTC	144.92706
BB93520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81935	WGS84	BB93520	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93520_Pcan_Bot2011_IGS	MD63	1	BB93520_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93520	Sample	BB93520	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD63	IGS	45	BB93520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD63	CGTAATCCTCAGTACTGGCTA	144.92706
BB93520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81935	WGS84	BB93520	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.58	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93520_Sspa_Oph2007_COI	MD63	1	BB93520_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93520	Sample	BB93520	targeted	Present	Sample	Sspa_Oph2007_COI	MD63	COI	45	BB93520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD63	GAAGGCCTTCCGATGATC	144.92706
BB93520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81935	WGS84	BB93520	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93520_Upin_Bot2011_COI	MD63	1	BB93520_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93520	Sample	BB93520	targeted	Absent	Sample	Upin_Bot2011_COI	MD63	COI	45	BB93520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD63	CTTTAATTACAGCGTTTTTATTGTTGT	144.92706
BB93521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82012	WGS84	BB93521	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93521_Mgig_Bot2012_COI	MD59	1	BB93521_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93521	Sample	BB93521	targeted	Absent	Sample	Mgig_Bot2012_COI	MD59	COI	45	BB93521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD59	TCTTATTCGTTGGAGACTTTATAACCCT	144.92885
BB93522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93522	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.594	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93522_Cmae_Bot2011_COI	MD57	1	BB93522_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93522	Sample	BB93522	targeted	Present	Sample	Cmae_Bot2011_COI	MD57	COI	45	BB93522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD57	ATGAACAGTCTATCCTCCTTTAG	144.93141
BB93522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81993	WGS84	BB93522	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93522_Msal_Bot2012_COI	MD57	1	BB93522_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93522	Sample	BB93522	targeted	Absent	Sample	Msal_Bot2012_COI	MD57	COI	45	BB93522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD57	GYTAGTTCCRATGATGTTAGCTG	144.93141
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.306	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93524_Aamu_Bax2006_COI	MD51	1	BB93524_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Present	Sample	Aamu_Bax2006_COI	MD51	COI	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	GCACAACCGGGATCTTTACTTC	144.91089
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93524_Pcan_Bot2011_IGS	MD51	1	BB93524_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD51	IGS	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	CGTAATCCTCAGTACTGGCTA	144.91089
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.206	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93524_Sspa_Oph2007_COI	MD51	1	BB93524_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Present	Sample	Sspa_Oph2007_COI	MD51	COI	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	GAAGGCCTTCCGATGATC	144.91089
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.451	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93524_Upin_Bot2011_COI	MD51	1	BB93524_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Present	Sample	Upin_Bot2011_COI	MD51	COI	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	CTTTAATTACAGCGTTTTTATTGTTGT	144.91089
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93525_Mgig_Bot2012_COI	MD49	1	BB93525_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Absent	Sample	Mgig_Bot2012_COI	MD49	COI	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	TCTTATTCGTTGGAGACTTTATAACCCT	144.91331
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93526_Cmae_Bot2011_COI	MD48	1	BB93526_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Absent	Sample	Cmae_Bot2011_COI	MD48	COI	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	ATGAACAGTCTATCCTCCTTTAG	144.91415
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93526_Hsan_Wil2021_COI	MD48	1	BB93526_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Absent	Sample	Hsan_Wil2021_COI	MD48	COI	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	CCTGGGCCGGTATAGTAGGT	144.91415
BB93526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86072	WGS84	BB93526	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93526_Msal_Bot2012_COI	MD48	1	BB93526_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93526	Sample	BB93526	targeted	Absent	Sample	Msal_Bot2012_COI	MD48	COI	45	BB93526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD48	GYTAGTTCCRATGATGTTAGCTG	144.91415
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.712	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93528_Aamu_Bax2006_COI	MD43	1	BB93528_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Present	Sample	Aamu_Bax2006_COI	MD43	COI	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	GCACAACCGGGATCTTTACTTC	144.91599
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93528_Pcan_Bot2011_IGS	MD43	1	BB93528_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD43	IGS	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	CGTAATCCTCAGTACTGGCTA	144.91599
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.66	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93528_Sspa_Oph2007_COI	MD43	1	BB93528_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Present	Sample	Sspa_Oph2007_COI	MD43	COI	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	GAAGGCCTTCCGATGATC	144.91599
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.142	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93528_Upin_Bot2011_COI	MD43	1	BB93528_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Present	Sample	Upin_Bot2011_COI	MD43	COI	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	CTTTAATTACAGCGTTTTTATTGTTGT	144.91599
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93529_Mgig_Bot2012_COI	MD39	1	BB93529_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Absent	Sample	Mgig_Bot2012_COI	MD39	COI	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	TCTTATTCGTTGGAGACTTTATAACCCT	144.91853
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.88	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93531_Aamu_Bax2006_COI	MD37	1	BB93531_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Present	Sample	Aamu_Bax2006_COI	MD37	COI	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	GCACAACCGGGATCTTTACTTC	144.9044
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93531_Pcan_Bot2011_IGS	MD37	1	BB93531_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD37	IGS	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	CGTAATCCTCAGTACTGGCTA	144.9044
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.6	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93531_Sspa_Oph2007_COI	MD37	1	BB93531_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Present	Sample	Sspa_Oph2007_COI	MD37	COI	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	GAAGGCCTTCCGATGATC	144.9044
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93531_Upin_Bot2011_COI	MD37	1	BB93531_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Absent	Sample	Upin_Bot2011_COI	MD37	COI	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	CTTTAATTACAGCGTTTTTATTGTTGT	144.9044
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93532_Mgig_Bot2012_COI	MD36	1	BB93532_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Absent	Sample	Mgig_Bot2012_COI	MD36	COI	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	TCTTATTCGTTGGAGACTTTATAACCCT	144.90613
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.136	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93533_Cmae_Bot2011_COI	MD33	1	BB93533_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Present	Sample	Cmae_Bot2011_COI	MD33	COI	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	ATGAACAGTCTATCCTCCTTTAG	144.91481
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93533_Hsan_Wil2021_COI	MD33	1	BB93533_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Absent	Sample	Hsan_Wil2021_COI	MD33	COI	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	CCTGGGCCGGTATAGTAGGT	144.91481
BB93533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86409	WGS84	BB93533	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93533_Msal_Bot2012_COI	MD33	1	BB93533_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93533	Sample	BB93533	targeted	Absent	Sample	Msal_Bot2012_COI	MD33	COI	45	BB93533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD33	GYTAGTTCCRATGATGTTAGCTG	144.91481
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.987	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93535_Aamu_Bax2006_COI	MD26	1	BB93535_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Present	Sample	Aamu_Bax2006_COI	MD26	COI	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	GCACAACCGGGATCTTTACTTC	144.91145
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93535_Pcan_Bot2011_IGS	MD26	1	BB93535_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD26	IGS	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	CGTAATCCTCAGTACTGGCTA	144.91145
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	26.317	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93535_Sspa_Oph2007_COI	MD26	1	BB93535_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Present	Sample	Sspa_Oph2007_COI	MD26	COI	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	GAAGGCCTTCCGATGATC	144.91145
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.662	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93535_Upin_Bot2011_COI	MD26	1	BB93535_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Present	Sample	Upin_Bot2011_COI	MD26	COI	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	CTTTAATTACAGCGTTTTTATTGTTGT	144.91145
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93536_Mgig_Bot2012_COI	MD23	1	BB93536_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Absent	Sample	Mgig_Bot2012_COI	MD23	COI	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	TCTTATTCGTTGGAGACTTTATAACCCT	144.90969
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.245	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93537_Cmae_Bot2011_COI	MD21	1	BB93537_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Present	Sample	Cmae_Bot2011_COI	MD21	COI	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	ATGAACAGTCTATCCTCCTTTAG	144.90981
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93537_Hsan_Wil2021_COI	MD21	1	BB93537_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Absent	Sample	Hsan_Wil2021_COI	MD21	COI	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	CCTGGGCCGGTATAGTAGGT	144.90981
BB93537	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8472	WGS84	BB93537	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93537_Msal_Bot2012_COI	MD21	1	BB93537_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93537	Sample	BB93537	targeted	Absent	Sample	Msal_Bot2012_COI	MD21	COI	45	BB93537	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD21	GYTAGTTCCRATGATGTTAGCTG	144.90981
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.134	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93539_Aamu_Bax2006_COI	MD17	1	BB93539_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Present	Sample	Aamu_Bax2006_COI	MD17	COI	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	GCACAACCGGGATCTTTACTTC	144.90621
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93539_Pcan_Bot2011_IGS	MD17	1	BB93539_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD17	IGS	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	CGTAATCCTCAGTACTGGCTA	144.90621
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.657	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93539_Sspa_Oph2007_COI	MD17	1	BB93539_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Present	Sample	Sspa_Oph2007_COI	MD17	COI	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	GAAGGCCTTCCGATGATC	144.90621
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.805	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93539_Upin_Bot2011_COI	MD17	1	BB93539_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Present	Sample	Upin_Bot2011_COI	MD17	COI	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	CTTTAATTACAGCGTTTTTATTGTTGT	144.90621
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93540_Cmae_Bot2011_COI	MD16	1	BB93540_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Absent	Sample	Cmae_Bot2011_COI	MD16	COI	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	ATGAACAGTCTATCCTCCTTTAG	144.90716
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93540_Hsan_Wil2021_COI	MD16	1	BB93540_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Absent	Sample	Hsan_Wil2021_COI	MD16	COI	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	CCTGGGCCGGTATAGTAGGT	144.90716
BB93540	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84345	WGS84	BB93540	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93540_Msal_Bot2012_COI	MD16	1	BB93540_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93540	Sample	BB93540	targeted	Absent	Sample	Msal_Bot2012_COI	MD16	COI	45	BB93540	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD16	GYTAGTTCCRATGATGTTAGCTG	144.90716
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.947	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93542_Aamu_Bax2006_COI	MD12	1	BB93542_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Present	Sample	Aamu_Bax2006_COI	MD12	COI	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	GCACAACCGGGATCTTTACTTC	144.90411
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93542_Pcan_Bot2011_IGS	MD12	1	BB93542_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD12	IGS	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	CGTAATCCTCAGTACTGGCTA	144.90411
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.295	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93542_Sspa_Oph2007_COI	MD12	1	BB93542_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Present	Sample	Sspa_Oph2007_COI	MD12	COI	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	GAAGGCCTTCCGATGATC	144.90411
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.956	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93542_Upin_Bot2011_COI	MD12	1	BB93542_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Present	Sample	Upin_Bot2011_COI	MD12	COI	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	CTTTAATTACAGCGTTTTTATTGTTGT	144.90411
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93543_Mgig_Bot2012_COI	MD11	1	BB93543_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Absent	Sample	Mgig_Bot2012_COI	MD11	COI	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	TCTTATTCGTTGGAGACTTTATAACCCT	144.92611
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93544_Cmae_Bot2011_COI	MD9	1	BB93544_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Absent	Sample	Cmae_Bot2011_COI	MD9	COI	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	ATGAACAGTCTATCCTCCTTTAG	144.92807
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93544_Hsan_Wil2021_COI	MD9	1	BB93544_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Absent	Sample	Hsan_Wil2021_COI	MD9	COI	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	CCTGGGCCGGTATAGTAGGT	144.92807
BB93544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84261	WGS84	BB93544	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93544_Msal_Bot2012_COI	MD9	1	BB93544_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93544	Sample	BB93544	targeted	Absent	Sample	Msal_Bot2012_COI	MD9	COI	45	BB93544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD9	GYTAGTTCCRATGATGTTAGCTG	144.92807
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.858	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93546_Aamu_Bax2006_COI	MD6	1	BB93546_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Present	Sample	Aamu_Bax2006_COI	MD6	COI	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	GCACAACCGGGATCTTTACTTC	144.92993
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93546_Pcan_Bot2011_IGS	MD6	1	BB93546_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD6	IGS	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	CGTAATCCTCAGTACTGGCTA	144.92993
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.985	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93546_Sspa_Oph2007_COI	MD6	1	BB93546_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Present	Sample	Sspa_Oph2007_COI	MD6	COI	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	GAAGGCCTTCCGATGATC	144.92993
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.068	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93546_Upin_Bot2011_COI	MD6	1	BB93546_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Present	Sample	Upin_Bot2011_COI	MD6	COI	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	CTTTAATTACAGCGTTTTTATTGTTGT	144.92993
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93547_Mgig_Bot2012_COI	MD4	1	BB93547_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Absent	Sample	Mgig_Bot2012_COI	MD4	COI	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	TCTTATTCGTTGGAGACTTTATAACCCT	144.92859
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.413	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93548_Cmae_Bot2011_COI	MD3	1	BB93548_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Present	Sample	Cmae_Bot2011_COI	MD3	COI	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	ATGAACAGTCTATCCTCCTTTAG	144.93105
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93548_Hsan_Wil2021_COI	MD3	1	BB93548_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Absent	Sample	Hsan_Wil2021_COI	MD3	COI	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	CCTGGGCCGGTATAGTAGGT	144.93105
BB93548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84723	WGS84	BB93548	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93548_Msal_Bot2012_COI	MD3	1	BB93548_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93548	Sample	BB93548	targeted	Absent	Sample	Msal_Bot2012_COI	MD3	COI	45	BB93548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD3	GYTAGTTCCRATGATGTTAGCTG	144.93105
BB93550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37147	WGS84	BB93550	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93550_Mgig_Bot2012_COI	BD55	1	BB93550_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93550	Sample	BB93550	targeted	Absent	Sample	Mgig_Bot2012_COI	BD55	COI	45	BB93550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD55	TCTTATTCGTTGGAGACTTTATAACCCT	153.1703
BB93551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37878	WGS84	BB93551	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93551_Cmae_Bot2011_COI	BD50	1	BB93551_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93551	Sample	BB93551	targeted	Absent	Sample	Cmae_Bot2011_COI	BD50	COI	45	BB93551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD50	ATGAACAGTCTATCCTCCTTTAG	153.1647
BB93551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37878	WGS84	BB93551	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93551_Msal_Bot2012_COI	BD50	1	BB93551_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93551	Sample	BB93551	targeted	Absent	Sample	Msal_Bot2012_COI	BD50	COI	45	BB93551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD50	GYTAGTTCCRATGATGTTAGCTG	153.1647
BB93553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43042	WGS84	BB93553	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93553_Aamu_Bax2006_COI	BD25	1	BB93553_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93553	Sample	BB93553	targeted	Absent	Sample	Aamu_Bax2006_COI	BD25	COI	45	BB93553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD25	GCACAACCGGGATCTTTACTTC	153.12364
BB93553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43042	WGS84	BB93553	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93553_Pcan_Bot2011_IGS	BD25	1	BB93553_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93553	Sample	BB93553	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD25	IGS	45	BB93553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD25	CGTAATCCTCAGTACTGGCTA	153.12364
BB93553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43042	WGS84	BB93553	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93553_Sspa_Oph2007_COI	BD25	1	BB93553_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93553	Sample	BB93553	targeted	Absent	Sample	Sspa_Oph2007_COI	BD25	COI	45	BB93553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD25	GAAGGCCTTCCGATGATC	153.12364
BB93553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43042	WGS84	BB93553	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93553_Upin_Bot2011_COI	BD25	1	BB93553_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93553	Sample	BB93553	targeted	Absent	Sample	Upin_Bot2011_COI	BD25	COI	45	BB93553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD25	CTTTAATTACAGCGTTTTTATTGTTGT	153.12364
BB93554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44768	WGS84	BB93554	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93554_Mgig_Bot2012_COI	BD15	1	BB93554_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93554	Sample	BB93554	targeted	Absent	Sample	Mgig_Bot2012_COI	BD15	COI	45	BB93554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD15	TCTTATTCGTTGGAGACTTTATAACCCT	153.0949
BB93555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3571	WGS84	BB93555	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93555_Cmae_Bot2011_COI	BD64	1	BB93555_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93555	Sample	BB93555	targeted	Absent	Sample	Cmae_Bot2011_COI	BD64	COI	45	BB93555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD64	ATGAACAGTCTATCCTCCTTTAG	153.18065
BB93555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3571	WGS84	BB93555	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93555_Msal_Bot2012_COI	BD64	1	BB93555_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93555	Sample	BB93555	targeted	Absent	Sample	Msal_Bot2012_COI	BD64	COI	45	BB93555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD64	GYTAGTTCCRATGATGTTAGCTG	153.18065
BB93557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36529	WGS84	BB93557	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93557_Aamu_Bax2006_COI	BD59	1	BB93557_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93557	Sample	BB93557	targeted	Absent	Sample	Aamu_Bax2006_COI	BD59	COI	45	BB93557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD59	GCACAACCGGGATCTTTACTTC	153.17455
BB93557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36529	WGS84	BB93557	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93557_Pcan_Bot2011_IGS	BD59	1	BB93557_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93557	Sample	BB93557	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD59	IGS	45	BB93557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD59	CGTAATCCTCAGTACTGGCTA	153.17455
BB93557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36529	WGS84	BB93557	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93557_Sspa_Oph2007_COI	BD59	1	BB93557_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93557	Sample	BB93557	targeted	Absent	Sample	Sspa_Oph2007_COI	BD59	COI	45	BB93557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD59	GAAGGCCTTCCGATGATC	153.17455
BB93557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36529	WGS84	BB93557	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93557_Upin_Bot2011_COI	BD59	1	BB93557_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93557	Sample	BB93557	targeted	Absent	Sample	Upin_Bot2011_COI	BD59	COI	45	BB93557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD59	CTTTAATTACAGCGTTTTTATTGTTGT	153.17455
BB93558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36857	WGS84	BB93558	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93558_Mgig_Bot2012_COI	BD57	1	BB93558_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93558	Sample	BB93558	targeted	Absent	Sample	Mgig_Bot2012_COI	BD57	COI	45	BB93558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD57	TCTTATTCGTTGGAGACTTTATAACCCT	153.17257
BB93559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37292	WGS84	BB93559	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93559_Cmae_Bot2011_COI	BD54	1	BB93559_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93559	Sample	BB93559	targeted	Absent	Sample	Cmae_Bot2011_COI	BD54	COI	45	BB93559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD54	ATGAACAGTCTATCCTCCTTTAG	153.16914
BB93559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37292	WGS84	BB93559	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93559_Msal_Bot2012_COI	BD54	1	BB93559_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93559	Sample	BB93559	targeted	Absent	Sample	Msal_Bot2012_COI	BD54	COI	45	BB93559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD54	GYTAGTTCCRATGATGTTAGCTG	153.16914
BB93560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37475	WGS84	BB93560	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93560_Aamu_Bax2006_COI	BD53	1	BB93560_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93560	Sample	BB93560	targeted	Absent	Sample	Aamu_Bax2006_COI	BD53	COI	45	BB93560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD53	GCACAACCGGGATCTTTACTTC	153.16829
BB93560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37475	WGS84	BB93560	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93560_Pcan_Bot2011_IGS	BD53	1	BB93560_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93560	Sample	BB93560	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD53	IGS	45	BB93560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD53	CGTAATCCTCAGTACTGGCTA	153.16829
BB93560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37475	WGS84	BB93560	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93560_Sspa_Oph2007_COI	BD53	1	BB93560_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93560	Sample	BB93560	targeted	Absent	Sample	Sspa_Oph2007_COI	BD53	COI	45	BB93560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD53	GAAGGCCTTCCGATGATC	153.16829
BB93560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37475	WGS84	BB93560	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93560_Upin_Bot2011_COI	BD53	1	BB93560_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93560	Sample	BB93560	targeted	Absent	Sample	Upin_Bot2011_COI	BD53	COI	45	BB93560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD53	CTTTAATTACAGCGTTTTTATTGTTGT	153.16829
BB93561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3773	WGS84	BB93561	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93561_Mgig_Bot2012_COI	BD51	1	BB93561_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93561	Sample	BB93561	targeted	Absent	Sample	Mgig_Bot2012_COI	BD51	COI	45	BB93561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD51	TCTTATTCGTTGGAGACTTTATAACCCT	153.16576
BB93562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38117	WGS84	BB93562	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93562_Cmae_Bot2011_COI	BD48	1	BB93562_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93562	Sample	BB93562	targeted	Absent	Sample	Cmae_Bot2011_COI	BD48	COI	45	BB93562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD48	ATGAACAGTCTATCCTCCTTTAG	153.16243
BB93562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38117	WGS84	BB93562	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93562_Msal_Bot2012_COI	BD48	1	BB93562_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93562	Sample	BB93562	targeted	Absent	Sample	Msal_Bot2012_COI	BD48	COI	45	BB93562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD48	GYTAGTTCCRATGATGTTAGCTG	153.16243
BB93564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38628	WGS84	BB93564	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93564_Aamu_Bax2006_COI	BD44	1	BB93564_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93564	Sample	BB93564	targeted	Absent	Sample	Aamu_Bax2006_COI	BD44	COI	45	BB93564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD44	GCACAACCGGGATCTTTACTTC	153.15955
BB93564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38628	WGS84	BB93564	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93564_Pcan_Bot2011_IGS	BD44	1	BB93564_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93564	Sample	BB93564	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD44	IGS	45	BB93564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD44	CGTAATCCTCAGTACTGGCTA	153.15955
BB93564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38628	WGS84	BB93564	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93564_Sspa_Oph2007_COI	BD44	1	BB93564_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93564	Sample	BB93564	targeted	Absent	Sample	Sspa_Oph2007_COI	BD44	COI	45	BB93564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD44	GAAGGCCTTCCGATGATC	153.15955
BB93564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38628	WGS84	BB93564	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93564_Upin_Bot2011_COI	BD44	1	BB93564_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93564	Sample	BB93564	targeted	Absent	Sample	Upin_Bot2011_COI	BD44	COI	45	BB93564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD44	CTTTAATTACAGCGTTTTTATTGTTGT	153.15955
BB93565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.39045	WGS84	BB93565	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93565_Mgig_Bot2012_COI	BD42	1	BB93565_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93565	Sample	BB93565	targeted	Absent	Sample	Mgig_Bot2012_COI	BD42	COI	45	BB93565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD42	TCTTATTCGTTGGAGACTTTATAACCCT	153.15746
BB93566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40067	WGS84	BB93566	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93566_Cmae_Bot2011_COI	BD41	1	BB93566_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93566	Sample	BB93566	targeted	Absent	Sample	Cmae_Bot2011_COI	BD41	COI	45	BB93566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD41	ATGAACAGTCTATCCTCCTTTAG	153.16346
BB93566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40067	WGS84	BB93566	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93566_Msal_Bot2012_COI	BD41	1	BB93566_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93566	Sample	BB93566	targeted	Absent	Sample	Msal_Bot2012_COI	BD41	COI	45	BB93566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD41	GYTAGTTCCRATGATGTTAGCTG	153.16346
BB93568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40102	WGS84	BB93568	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93568_Aamu_Bax2006_COI	BD37	1	BB93568_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93568	Sample	BB93568	targeted	Absent	Sample	Aamu_Bax2006_COI	BD37	COI	45	BB93568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD37	GCACAACCGGGATCTTTACTTC	153.15883
BB93568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40102	WGS84	BB93568	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93568_Pcan_Bot2011_IGS	BD37	1	BB93568_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93568	Sample	BB93568	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD37	IGS	45	BB93568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD37	CGTAATCCTCAGTACTGGCTA	153.15883
BB93568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40102	WGS84	BB93568	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93568_Sspa_Oph2007_COI	BD37	1	BB93568_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93568	Sample	BB93568	targeted	Absent	Sample	Sspa_Oph2007_COI	BD37	COI	45	BB93568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD37	GAAGGCCTTCCGATGATC	153.15883
BB93568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40102	WGS84	BB93568	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93568_Upin_Bot2011_COI	BD37	1	BB93568_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93568	Sample	BB93568	targeted	Absent	Sample	Upin_Bot2011_COI	BD37	COI	45	BB93568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD37	CTTTAATTACAGCGTTTTTATTGTTGT	153.15883
BB93569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40516	WGS84	BB93569	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93569_Mgig_Bot2012_COI	BD33	1	BB93569_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93569	Sample	BB93569	targeted	Absent	Sample	Mgig_Bot2012_COI	BD33	COI	45	BB93569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD33	TCTTATTCGTTGGAGACTTTATAACCCT	153.15148
BB93571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.41593	WGS84	BB93571	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93571_Aamu_Bax2006_COI	BD31	1	BB93571_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93571	Sample	BB93571	targeted	Absent	Sample	Aamu_Bax2006_COI	BD31	COI	45	BB93571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD31	GCACAACCGGGATCTTTACTTC	153.14258
BB93571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.41593	WGS84	BB93571	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93571_Pcan_Bot2011_IGS	BD31	1	BB93571_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93571	Sample	BB93571	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD31	IGS	45	BB93571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD31	CGTAATCCTCAGTACTGGCTA	153.14258
BB93571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.41593	WGS84	BB93571	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93571_Sspa_Oph2007_COI	BD31	1	BB93571_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93571	Sample	BB93571	targeted	Absent	Sample	Sspa_Oph2007_COI	BD31	COI	45	BB93571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD31	GAAGGCCTTCCGATGATC	153.14258
BB93571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.41593	WGS84	BB93571	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93571_Upin_Bot2011_COI	BD31	1	BB93571_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93571	Sample	BB93571	targeted	Absent	Sample	Upin_Bot2011_COI	BD31	COI	45	BB93571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD31	CTTTAATTACAGCGTTTTTATTGTTGT	153.14258
BB93572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42178	WGS84	BB93572	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93572_Mgig_Bot2012_COI	BD29	1	BB93572_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93572	Sample	BB93572	targeted	Absent	Sample	Mgig_Bot2012_COI	BD29	COI	45	BB93572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD29	TCTTATTCGTTGGAGACTTTATAACCCT	153.13521
BB93573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42503	WGS84	BB93573	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93573_Cmae_Bot2011_COI	BD27	1	BB93573_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93573	Sample	BB93573	targeted	Absent	Sample	Cmae_Bot2011_COI	BD27	COI	45	BB93573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD27	ATGAACAGTCTATCCTCCTTTAG	153.12793
BB93573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42503	WGS84	BB93573	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93573_Msal_Bot2012_COI	BD27	1	BB93573_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93573	Sample	BB93573	targeted	Absent	Sample	Msal_Bot2012_COI	BD27	COI	45	BB93573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD27	GYTAGTTCCRATGATGTTAGCTG	153.12793
BB93575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42982	WGS84	BB93575	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93575_Aamu_Bax2006_COI	BD21	1	BB93575_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93575	Sample	BB93575	targeted	Absent	Sample	Aamu_Bax2006_COI	BD21	COI	45	BB93575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD21	GCACAACCGGGATCTTTACTTC	153.12339
BB93575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42982	WGS84	BB93575	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93575_Pcan_Bot2011_IGS	BD21	1	BB93575_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93575	Sample	BB93575	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD21	IGS	45	BB93575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD21	CGTAATCCTCAGTACTGGCTA	153.12339
BB93575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42982	WGS84	BB93575	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93575_Sspa_Oph2007_COI	BD21	1	BB93575_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93575	Sample	BB93575	targeted	Absent	Sample	Sspa_Oph2007_COI	BD21	COI	45	BB93575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD21	GAAGGCCTTCCGATGATC	153.12339
BB93575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42982	WGS84	BB93575	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93575_Upin_Bot2011_COI	BD21	1	BB93575_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93575	Sample	BB93575	targeted	Absent	Sample	Upin_Bot2011_COI	BD21	COI	45	BB93575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD21	CTTTAATTACAGCGTTTTTATTGTTGT	153.12339
BB93576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43122	WGS84	BB93576	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93576_Mgig_Bot2012_COI	BD18	1	BB93576_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93576	Sample	BB93576	targeted	Absent	Sample	Mgig_Bot2012_COI	BD18	COI	45	BB93576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD18	TCTTATTCGTTGGAGACTTTATAACCCT	153.11783
BB93577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44736	WGS84	BB93577	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93577_Cmae_Bot2011_COI	BD16	1	BB93577_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93577	Sample	BB93577	targeted	Absent	Sample	Cmae_Bot2011_COI	BD16	COI	45	BB93577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD16	ATGAACAGTCTATCCTCCTTTAG	153.09726
BB93577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44736	WGS84	BB93577	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93577_Msal_Bot2012_COI	BD16	1	BB93577_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93577	Sample	BB93577	targeted	Absent	Sample	Msal_Bot2012_COI	BD16	COI	45	BB93577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD16	GYTAGTTCCRATGATGTTAGCTG	153.09726
BB93579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44615	WGS84	BB93579	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93579_Aamu_Bax2006_COI	BD9	1	BB93579_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93579	Sample	BB93579	targeted	Absent	Sample	Aamu_Bax2006_COI	BD9	COI	45	BB93579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD9	GCACAACCGGGATCTTTACTTC	153.07929
BB93579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44615	WGS84	BB93579	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93579_Pcan_Bot2011_IGS	BD9	1	BB93579_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93579	Sample	BB93579	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD9	IGS	45	BB93579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD9	CGTAATCCTCAGTACTGGCTA	153.07929
BB93579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44615	WGS84	BB93579	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93579_Sspa_Oph2007_COI	BD9	1	BB93579_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93579	Sample	BB93579	targeted	Absent	Sample	Sspa_Oph2007_COI	BD9	COI	45	BB93579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD9	GAAGGCCTTCCGATGATC	153.07929
BB93579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44615	WGS84	BB93579	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93579_Upin_Bot2011_COI	BD9	1	BB93579_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93579	Sample	BB93579	targeted	Absent	Sample	Upin_Bot2011_COI	BD9	COI	45	BB93579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD9	CTTTAATTACAGCGTTTTTATTGTTGT	153.07929
BB93580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44522	WGS84	BB93580	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93580_Cmae_Bot2011_COI	BD8	1	BB93580_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93580	Sample	BB93580	targeted	Absent	Sample	Cmae_Bot2011_COI	BD8	COI	45	BB93580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD8	ATGAACAGTCTATCCTCCTTTAG	153.0791
BB93580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44522	WGS84	BB93580	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93580_Msal_Bot2012_COI	BD8	1	BB93580_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93580	Sample	BB93580	targeted	Absent	Sample	Msal_Bot2012_COI	BD8	COI	45	BB93580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD8	GYTAGTTCCRATGATGTTAGCTG	153.0791
BB93582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44446	WGS84	BB93582	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93582_Aamu_Bax2006_COI	BD6	1	BB93582_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93582	Sample	BB93582	targeted	Absent	Sample	Aamu_Bax2006_COI	BD6	COI	45	BB93582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD6	GCACAACCGGGATCTTTACTTC	153.07696
BB93582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44446	WGS84	BB93582	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93582_Pcan_Bot2011_IGS	BD6	1	BB93582_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93582	Sample	BB93582	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD6	IGS	45	BB93582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD6	CGTAATCCTCAGTACTGGCTA	153.07696
BB93582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44446	WGS84	BB93582	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93582_Sspa_Oph2007_COI	BD6	1	BB93582_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93582	Sample	BB93582	targeted	Absent	Sample	Sspa_Oph2007_COI	BD6	COI	45	BB93582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD6	GAAGGCCTTCCGATGATC	153.07696
BB93582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44446	WGS84	BB93582	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93582_Upin_Bot2011_COI	BD6	1	BB93582_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93582	Sample	BB93582	targeted	Absent	Sample	Upin_Bot2011_COI	BD6	COI	45	BB93582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD6	CTTTAATTACAGCGTTTTTATTGTTGT	153.07696
BB93583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44569	WGS84	BB93583	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93583_Mgig_Bot2012_COI	BD4	1	BB93583_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93583	Sample	BB93583	targeted	Absent	Sample	Mgig_Bot2012_COI	BD4	COI	45	BB93583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD4	TCTTATTCGTTGGAGACTTTATAACCCT	153.07546
BB93584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44385	WGS84	BB93584	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93584_Cmae_Bot2011_COI	BD3	1	BB93584_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93584	Sample	BB93584	targeted	Absent	Sample	Cmae_Bot2011_COI	BD3	COI	45	BB93584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD3	ATGAACAGTCTATCCTCCTTTAG	153.07508
BB93584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44385	WGS84	BB93584	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93584_Msal_Bot2012_COI	BD3	1	BB93584_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93584	Sample	BB93584	targeted	Absent	Sample	Msal_Bot2012_COI	BD3	COI	45	BB93584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD3	GYTAGTTCCRATGATGTTAGCTG	153.07508
BB93586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24295	WGS84	BB93586	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93586_Aamu_Bax2006_COI	YD140	1	BB93586_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93586	Sample	BB93586	targeted	Absent	Sample	Aamu_Bax2006_COI	YD140	COI	45	BB93586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD140	GCACAACCGGGATCTTTACTTC	149.30158
BB93586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24295	WGS84	BB93586	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93586_Pcan_Bot2011_IGS	YD140	1	BB93586_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93586	Sample	BB93586	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD140	IGS	45	BB93586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD140	CGTAATCCTCAGTACTGGCTA	149.30158
BB93586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24295	WGS84	BB93586	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93586_Sspa_Oph2007_COI	YD140	1	BB93586_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93586	Sample	BB93586	targeted	Absent	Sample	Sspa_Oph2007_COI	YD140	COI	45	BB93586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD140	GAAGGCCTTCCGATGATC	149.30158
BB93586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24295	WGS84	BB93586	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93586_Upin_Bot2011_COI	YD140	1	BB93586_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93586	Sample	BB93586	targeted	Absent	Sample	Upin_Bot2011_COI	YD140	COI	45	BB93586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD140	CTTTAATTACAGCGTTTTTATTGTTGT	149.30158
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93587_Cjap_Sim2018_COI	YD138	1	BB93587_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Cjap_Sim2018_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93587_Esin_And2018_Cyb	YD138	1	BB93587_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Esin_And2018_Cyb	YD138	Cytb	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	ACCCCTCCTCATATCCAACCA	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93587_Mgig_Bot2012_COI	YD138	1	BB93587_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Mgig_Bot2012_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	TCTTATTCGTTGGAGACTTTATAACCCT	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93587_Mjap_Gib2022_COI	YD138	1	BB93587_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Mjap_Gib2022_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	AATTTAATTGTGACTAGGCATGGTCTG	149.31354
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93588_Cmae_Bot2011_COI	YD136	1	BB93588_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Cmae_Bot2011_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	ATGAACAGTCTATCCTCCTTTAG	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93588_Hsan_Wil2021_COI	YD136	1	BB93588_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Hsan_Wil2021_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	CCTGGGCCGGTATAGTAGGT	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93588_Mros_Gib2022_COI	YD136	1	BB93588_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Mros_Gib2022_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93588_Msal_Bot2012_COI	YD136	1	BB93588_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Msal_Bot2012_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	GYTAGTTCCRATGATGTTAGCTG	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93588_Pvir_Dia2013_COI	YD136	1	BB93588_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Pvir_Dia2013_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	CTTAGTGGCATTAATTCGDAATCC	149.31324
BB93588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26064	WGS84	BB93588	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93588_Rhar_Sim2018_COI	YD136	1	BB93588_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93588	Sample	BB93588	targeted	Absent	Sample	Rhar_Sim2018_COI	YD136	COI	45	BB93588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD136	CTGCTGCTATTGCTCATG	149.31324
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93590_Cjap_Sim2018_COI	YD132	1	BB93590_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Cjap_Sim2018_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93590_Esin_And2018_Cyb	YD132	1	BB93590_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Esin_And2018_Cyb	YD132	Cytb	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	ACCCCTCCTCATATCCAACCA	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93590_Mgig_Bot2012_COI	YD132	1	BB93590_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Mgig_Bot2012_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	TCTTATTCGTTGGAGACTTTATAACCCT	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93590_Mjap_Gib2022_COI	YD132	1	BB93590_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Mjap_Gib2022_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	AATTTAATTGTGACTAGGCATGGTCTG	149.31331
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93591_Cmae_Bot2011_COI	YD131	1	BB93591_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Cmae_Bot2011_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	ATGAACAGTCTATCCTCCTTTAG	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93591_Hsan_Wil2021_COI	YD131	1	BB93591_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Hsan_Wil2021_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	CCTGGGCCGGTATAGTAGGT	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93591_Mros_Gib2022_COI	YD131	1	BB93591_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Mros_Gib2022_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93591_Msal_Bot2012_COI	YD131	1	BB93591_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Msal_Bot2012_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	GYTAGTTCCRATGATGTTAGCTG	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93591_Pvir_Dia2013_COI	YD131	1	BB93591_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Pvir_Dia2013_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	CTTAGTGGCATTAATTCGDAATCC	149.3143
BB93591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26075	WGS84	BB93591	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93591_Rhar_Sim2018_COI	YD131	1	BB93591_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93591	Sample	BB93591	targeted	Absent	Sample	Rhar_Sim2018_COI	YD131	COI	45	BB93591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD131	CTGCTGCTATTGCTCATG	149.3143
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93593_Aamu_Bax2006_COI	YD128	1	BB93593_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Aamu_Bax2006_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	GCACAACCGGGATCTTTACTTC	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93593_Pcan_Bot2011_IGS	YD128	1	BB93593_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD128	IGS	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	CGTAATCCTCAGTACTGGCTA	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93593_Pper_Dia2013_COI	YD128	1	BB93593_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Pper_Dia2013_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	CTTAGTGGCATTAATTCGDAATCC	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93593_Sspa_Oph2007_COI	YD128	1	BB93593_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Sspa_Oph2007_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	GAAGGCCTTCCGATGATC	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93593_Upin_Bot2011_COI	YD128	1	BB93593_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Upin_Bot2011_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	CTTTAATTACAGCGTTTTTATTGTTGT	149.31464
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93594_Cjap_Sim2018_COI	YD127	1	BB93594_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Cjap_Sim2018_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93594_Esin_And2018_Cyb	YD127	1	BB93594_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Esin_And2018_Cyb	YD127	Cytb	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	ACCCCTCCTCATATCCAACCA	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93594_Mgig_Bot2012_COI	YD127	1	BB93594_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Mgig_Bot2012_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	TCTTATTCGTTGGAGACTTTATAACCCT	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93594_Mjap_Gib2022_COI	YD127	1	BB93594_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Mjap_Gib2022_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	AATTTAATTGTGACTAGGCATGGTCTG	149.31381
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93595_Cmae_Bot2011_COI	YD124	1	BB93595_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Cmae_Bot2011_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	ATGAACAGTCTATCCTCCTTTAG	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93595_Hsan_Wil2021_COI	YD124	1	BB93595_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Hsan_Wil2021_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	CCTGGGCCGGTATAGTAGGT	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93595_Mros_Gib2022_COI	YD124	1	BB93595_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Mros_Gib2022_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93595_Msal_Bot2012_COI	YD124	1	BB93595_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Msal_Bot2012_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	GYTAGTTCCRATGATGTTAGCTG	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93595_Pvir_Dia2013_COI	YD124	1	BB93595_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Pvir_Dia2013_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	CTTAGTGGCATTAATTCGDAATCC	149.31554
BB93595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26365	WGS84	BB93595	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93595_Rhar_Sim2018_COI	YD124	1	BB93595_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93595	Sample	BB93595	targeted	Absent	Sample	Rhar_Sim2018_COI	YD124	COI	45	BB93595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD124	CTGCTGCTATTGCTCATG	149.31554
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93597_Aamu_Bax2006_COI	YD122	1	BB93597_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Aamu_Bax2006_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	GCACAACCGGGATCTTTACTTC	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93597_Pcan_Bot2011_IGS	YD122	1	BB93597_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD122	IGS	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	CGTAATCCTCAGTACTGGCTA	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93597_Pper_Dia2013_COI	YD122	1	BB93597_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Pper_Dia2013_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	CTTAGTGGCATTAATTCGDAATCC	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93597_Sspa_Oph2007_COI	YD122	1	BB93597_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Sspa_Oph2007_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	GAAGGCCTTCCGATGATC	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93597_Upin_Bot2011_COI	YD122	1	BB93597_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Upin_Bot2011_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	CTTTAATTACAGCGTTTTTATTGTTGT	149.31396
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93598_Cjap_Sim2018_COI	YD118	1	BB93598_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Cjap_Sim2018_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93598_Esin_And2018_Cyb	YD118	1	BB93598_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Esin_And2018_Cyb	YD118	Cytb	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	ACCCCTCCTCATATCCAACCA	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93598_Mgig_Bot2012_COI	YD118	1	BB93598_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Mgig_Bot2012_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	TCTTATTCGTTGGAGACTTTATAACCCT	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93598_Mjap_Gib2022_COI	YD118	1	BB93598_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Mjap_Gib2022_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	AATTTAATTGTGACTAGGCATGGTCTG	149.31427
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93599_Cmae_Bot2011_COI	YD117	1	BB93599_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Cmae_Bot2011_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	ATGAACAGTCTATCCTCCTTTAG	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93599_Hsan_Wil2021_COI	YD117	1	BB93599_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Hsan_Wil2021_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	CCTGGGCCGGTATAGTAGGT	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93599_Msal_Bot2012_COI	YD117	1	BB93599_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Msal_Bot2012_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	GYTAGTTCCRATGATGTTAGCTG	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93599_Pvir_Dia2013_COI	YD117	1	BB93599_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Pvir_Dia2013_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	CTTAGTGGCATTAATTCGDAATCC	149.31395
BB93599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26495	WGS84	BB93599	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93599_Rhar_Sim2018_COI	YD117	1	BB93599_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93599	Sample	BB93599	targeted	Absent	Sample	Rhar_Sim2018_COI	YD117	COI	45	BB93599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD117	CTGCTGCTATTGCTCATG	149.31395
BB93601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26553	WGS84	BB93601	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93601_Aamu_Bax2006_COI	YD114	1	BB93601_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93601	Sample	BB93601	targeted	Absent	Sample	Aamu_Bax2006_COI	YD114	COI	45	BB93601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD114	GCACAACCGGGATCTTTACTTC	149.31434
BB93601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26553	WGS84	BB93601	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93601_Pcan_Bot2011_IGS	YD114	1	BB93601_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93601	Sample	BB93601	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD114	IGS	45	BB93601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD114	CGTAATCCTCAGTACTGGCTA	149.31434
BB93601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26553	WGS84	BB93601	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93601_Sspa_Oph2007_COI	YD114	1	BB93601_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93601	Sample	BB93601	targeted	Absent	Sample	Sspa_Oph2007_COI	YD114	COI	45	BB93601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD114	GAAGGCCTTCCGATGATC	149.31434
BB93601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26553	WGS84	BB93601	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93601_Upin_Bot2011_COI	YD114	1	BB93601_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93601	Sample	BB93601	targeted	Absent	Sample	Upin_Bot2011_COI	YD114	COI	45	BB93601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD114	CTTTAATTACAGCGTTTTTATTGTTGT	149.31434
BB93602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26621	WGS84	BB93602	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93602_Mgig_Bot2012_COI	YD113	1	BB93602_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93602	Sample	BB93602	targeted	Absent	Sample	Mgig_Bot2012_COI	YD113	COI	45	BB93602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD113	TCTTATTCGTTGGAGACTTTATAACCCT	149.31567
BB93603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25606	WGS84	BB93603	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93603_Cmae_Bot2011_COI	YD110	1	BB93603_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93603	Sample	BB93603	targeted	Absent	Sample	Cmae_Bot2011_COI	YD110	COI	45	BB93603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD110	ATGAACAGTCTATCCTCCTTTAG	149.30718
BB93603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25606	WGS84	BB93603	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93603_Msal_Bot2012_COI	YD110	1	BB93603_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93603	Sample	BB93603	targeted	Absent	Sample	Msal_Bot2012_COI	YD110	COI	45	BB93603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD110	GYTAGTTCCRATGATGTTAGCTG	149.30718
BB93605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26828	WGS84	BB93605	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93605_Aamu_Bax2006_COI	YD107	1	BB93605_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93605	Sample	BB93605	targeted	Absent	Sample	Aamu_Bax2006_COI	YD107	COI	45	BB93605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD107	GCACAACCGGGATCTTTACTTC	149.31404
BB93605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26828	WGS84	BB93605	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93605_Pcan_Bot2011_IGS	YD107	1	BB93605_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93605	Sample	BB93605	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD107	IGS	45	BB93605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD107	CGTAATCCTCAGTACTGGCTA	149.31404
BB93605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26828	WGS84	BB93605	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93605_Sspa_Oph2007_COI	YD107	1	BB93605_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93605	Sample	BB93605	targeted	Absent	Sample	Sspa_Oph2007_COI	YD107	COI	45	BB93605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD107	GAAGGCCTTCCGATGATC	149.31404
BB93605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26828	WGS84	BB93605	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93605_Upin_Bot2011_COI	YD107	1	BB93605_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93605	Sample	BB93605	targeted	Absent	Sample	Upin_Bot2011_COI	YD107	COI	45	BB93605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD107	CTTTAATTACAGCGTTTTTATTGTTGT	149.31404
BB93606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26754	WGS84	BB93606	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93606_Mgig_Bot2012_COI	YD106	1	BB93606_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93606	Sample	BB93606	targeted	Absent	Sample	Mgig_Bot2012_COI	YD106	COI	45	BB93606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD106	TCTTATTCGTTGGAGACTTTATAACCCT	149.31291
BB93607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26817	WGS84	BB93607	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93607_Cmae_Bot2011_COI	YD105	1	BB93607_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93607	Sample	BB93607	targeted	Absent	Sample	Cmae_Bot2011_COI	YD105	COI	45	BB93607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD105	ATGAACAGTCTATCCTCCTTTAG	149.31344
BB93607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26817	WGS84	BB93607	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93607_Msal_Bot2012_COI	YD105	1	BB93607_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93607	Sample	BB93607	targeted	Absent	Sample	Msal_Bot2012_COI	YD105	COI	45	BB93607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD105	GYTAGTTCCRATGATGTTAGCTG	149.31344
BB93609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25226	WGS84	BB93609	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93609_Aamu_Bax2006_COI	YD101	1	BB93609_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93609	Sample	BB93609	targeted	Absent	Sample	Aamu_Bax2006_COI	YD101	COI	45	BB93609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD101	GCACAACCGGGATCTTTACTTC	149.30554
BB93609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25226	WGS84	BB93609	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93609_Pcan_Bot2011_IGS	YD101	1	BB93609_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93609	Sample	BB93609	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD101	IGS	45	BB93609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD101	CGTAATCCTCAGTACTGGCTA	149.30554
BB93609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25226	WGS84	BB93609	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93609_Sspa_Oph2007_COI	YD101	1	BB93609_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93609	Sample	BB93609	targeted	Absent	Sample	Sspa_Oph2007_COI	YD101	COI	45	BB93609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD101	GAAGGCCTTCCGATGATC	149.30554
BB93609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25226	WGS84	BB93609	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93609_Upin_Bot2011_COI	YD101	1	BB93609_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93609	Sample	BB93609	targeted	Absent	Sample	Upin_Bot2011_COI	YD101	COI	45	BB93609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD101	CTTTAATTACAGCGTTTTTATTGTTGT	149.30554
BB93610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25367	WGS84	BB93610	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93610_Cmae_Bot2011_COI	YD99	1	BB93610_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93610	Sample	BB93610	targeted	Absent	Sample	Cmae_Bot2011_COI	YD99	COI	45	BB93610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD99	ATGAACAGTCTATCCTCCTTTAG	149.30558
BB93610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25367	WGS84	BB93610	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93610_Msal_Bot2012_COI	YD99	1	BB93610_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93610	Sample	BB93610	targeted	Absent	Sample	Msal_Bot2012_COI	YD99	COI	45	BB93610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD99	GYTAGTTCCRATGATGTTAGCTG	149.30558
BB93612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25435	WGS84	BB93612	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93612_Aamu_Bax2006_COI	YD97	1	BB93612_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93612	Sample	BB93612	targeted	Absent	Sample	Aamu_Bax2006_COI	YD97	COI	45	BB93612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD97	GCACAACCGGGATCTTTACTTC	149.30611
BB93612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25435	WGS84	BB93612	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93612_Pcan_Bot2011_IGS	YD97	1	BB93612_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93612	Sample	BB93612	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD97	IGS	45	BB93612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD97	CGTAATCCTCAGTACTGGCTA	149.30611
BB93612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25435	WGS84	BB93612	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93612_Sspa_Oph2007_COI	YD97	1	BB93612_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93612	Sample	BB93612	targeted	Absent	Sample	Sspa_Oph2007_COI	YD97	COI	45	BB93612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD97	GAAGGCCTTCCGATGATC	149.30611
BB93612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25435	WGS84	BB93612	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93612_Upin_Bot2011_COI	YD97	1	BB93612_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93612	Sample	BB93612	targeted	Absent	Sample	Upin_Bot2011_COI	YD97	COI	45	BB93612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD97	CTTTAATTACAGCGTTTTTATTGTTGT	149.30611
BB93613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25424	WGS84	BB93613	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93613_Mgig_Bot2012_COI	YD96	1	BB93613_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93613	Sample	BB93613	targeted	Absent	Sample	Mgig_Bot2012_COI	YD96	COI	45	BB93613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD96	TCTTATTCGTTGGAGACTTTATAACCCT	149.30705
BB93614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25563	WGS84	BB93614	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93614_Cmae_Bot2011_COI	YD94	1	BB93614_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93614	Sample	BB93614	targeted	Absent	Sample	Cmae_Bot2011_COI	YD94	COI	45	BB93614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD94	ATGAACAGTCTATCCTCCTTTAG	149.30793
BB93614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25563	WGS84	BB93614	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93614_Msal_Bot2012_COI	YD94	1	BB93614_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93614	Sample	BB93614	targeted	Absent	Sample	Msal_Bot2012_COI	YD94	COI	45	BB93614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD94	GYTAGTTCCRATGATGTTAGCTG	149.30793
BB93616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25693	WGS84	BB93616	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93616_Aamu_Bax2006_COI	YD91	1	BB93616_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93616	Sample	BB93616	targeted	Absent	Sample	Aamu_Bax2006_COI	YD91	COI	45	BB93616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD91	GCACAACCGGGATCTTTACTTC	149.30923
BB93616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25693	WGS84	BB93616	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93616_Pcan_Bot2011_IGS	YD91	1	BB93616_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93616	Sample	BB93616	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD91	IGS	45	BB93616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD91	CGTAATCCTCAGTACTGGCTA	149.30923
BB93616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25693	WGS84	BB93616	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93616_Sspa_Oph2007_COI	YD91	1	BB93616_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93616	Sample	BB93616	targeted	Absent	Sample	Sspa_Oph2007_COI	YD91	COI	45	BB93616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD91	GAAGGCCTTCCGATGATC	149.30923
BB93616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25693	WGS84	BB93616	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93616_Upin_Bot2011_COI	YD91	1	BB93616_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93616	Sample	BB93616	targeted	Absent	Sample	Upin_Bot2011_COI	YD91	COI	45	BB93616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD91	CTTTAATTACAGCGTTTTTATTGTTGT	149.30923
BB93617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25837	WGS84	BB93617	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93617_Mgig_Bot2012_COI	YD89	1	BB93617_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93617	Sample	BB93617	targeted	Absent	Sample	Mgig_Bot2012_COI	YD89	COI	45	BB93617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD89	TCTTATTCGTTGGAGACTTTATAACCCT	149.30937
BB93618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2592	WGS84	BB93618	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93618_Cmae_Bot2011_COI	YD88	1	BB93618_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93618	Sample	BB93618	targeted	Absent	Sample	Cmae_Bot2011_COI	YD88	COI	45	BB93618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD88	ATGAACAGTCTATCCTCCTTTAG	149.30901
BB93618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2592	WGS84	BB93618	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93618_Msal_Bot2012_COI	YD88	1	BB93618_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93618	Sample	BB93618	targeted	Absent	Sample	Msal_Bot2012_COI	YD88	COI	45	BB93618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD88	GYTAGTTCCRATGATGTTAGCTG	149.30901
BB93620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25977	WGS84	BB93620	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93620_Mgig_Bot2012_COI	YD84	1	BB93620_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93620	Sample	BB93620	targeted	Absent	Sample	Mgig_Bot2012_COI	YD84	COI	45	BB93620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD84	TCTTATTCGTTGGAGACTTTATAACCCT	149.31052
BB93621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26044	WGS84	BB93621	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93621_Cmae_Bot2011_COI	YD83	1	BB93621_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93621	Sample	BB93621	targeted	Absent	Sample	Cmae_Bot2011_COI	YD83	COI	45	BB93621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD83	ATGAACAGTCTATCCTCCTTTAG	149.31104
BB93621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26044	WGS84	BB93621	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93621_Msal_Bot2012_COI	YD83	1	BB93621_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93621	Sample	BB93621	targeted	Absent	Sample	Msal_Bot2012_COI	YD83	COI	45	BB93621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD83	GYTAGTTCCRATGATGTTAGCTG	149.31104
BB93623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26646	WGS84	BB93623	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93623_Aamu_Bax2006_COI	YD76	1	BB93623_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93623	Sample	BB93623	targeted	Absent	Sample	Aamu_Bax2006_COI	YD76	COI	45	BB93623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD76	GCACAACCGGGATCTTTACTTC	149.3169
BB93623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26646	WGS84	BB93623	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93623_Pcan_Bot2011_IGS	YD76	1	BB93623_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93623	Sample	BB93623	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD76	IGS	45	BB93623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD76	CGTAATCCTCAGTACTGGCTA	149.3169
BB93623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26646	WGS84	BB93623	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93623_Sspa_Oph2007_COI	YD76	1	BB93623_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93623	Sample	BB93623	targeted	Absent	Sample	Sspa_Oph2007_COI	YD76	COI	45	BB93623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD76	GAAGGCCTTCCGATGATC	149.3169
BB93623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26646	WGS84	BB93623	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93623_Upin_Bot2011_COI	YD76	1	BB93623_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93623	Sample	BB93623	targeted	Absent	Sample	Upin_Bot2011_COI	YD76	COI	45	BB93623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD76	CTTTAATTACAGCGTTTTTATTGTTGT	149.3169
BB93624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26709	WGS84	BB93624	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93624_Mgig_Bot2012_COI	YD75	1	BB93624_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93624	Sample	BB93624	targeted	Absent	Sample	Mgig_Bot2012_COI	YD75	COI	45	BB93624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD75	TCTTATTCGTTGGAGACTTTATAACCCT	149.31643
BB93625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26792	WGS84	BB93625	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93625_Cmae_Bot2011_COI	YD74	1	BB93625_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93625	Sample	BB93625	targeted	Absent	Sample	Cmae_Bot2011_COI	YD74	COI	45	BB93625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD74	ATGAACAGTCTATCCTCCTTTAG	149.31616
BB93625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26792	WGS84	BB93625	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93625_Msal_Bot2012_COI	YD74	1	BB93625_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93625	Sample	BB93625	targeted	Absent	Sample	Msal_Bot2012_COI	YD74	COI	45	BB93625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD74	GYTAGTTCCRATGATGTTAGCTG	149.31616
BB93627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25588	WGS84	BB93627	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93627_Aamu_Bax2006_COI	YD71	1	BB93627_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93627	Sample	BB93627	targeted	Absent	Sample	Aamu_Bax2006_COI	YD71	COI	45	BB93627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD71	GCACAACCGGGATCTTTACTTC	149.31227
BB93627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25588	WGS84	BB93627	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93627_Pcan_Bot2011_IGS	YD71	1	BB93627_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93627	Sample	BB93627	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD71	IGS	45	BB93627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD71	CGTAATCCTCAGTACTGGCTA	149.31227
BB93627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25588	WGS84	BB93627	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93627_Sspa_Oph2007_COI	YD71	1	BB93627_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93627	Sample	BB93627	targeted	Absent	Sample	Sspa_Oph2007_COI	YD71	COI	45	BB93627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD71	GAAGGCCTTCCGATGATC	149.31227
BB93627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25588	WGS84	BB93627	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93627_Upin_Bot2011_COI	YD71	1	BB93627_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93627	Sample	BB93627	targeted	Absent	Sample	Upin_Bot2011_COI	YD71	COI	45	BB93627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD71	CTTTAATTACAGCGTTTTTATTGTTGT	149.31227
BB93628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25665	WGS84	BB93628	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93628_Mgig_Bot2012_COI	YD70	1	BB93628_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93628	Sample	BB93628	targeted	Absent	Sample	Mgig_Bot2012_COI	YD70	COI	45	BB93628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD70	TCTTATTCGTTGGAGACTTTATAACCCT	149.31193
BB93629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25774	WGS84	BB93629	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93629_Cmae_Bot2011_COI	YD69	1	BB93629_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93629	Sample	BB93629	targeted	Absent	Sample	Cmae_Bot2011_COI	YD69	COI	45	BB93629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD69	ATGAACAGTCTATCCTCCTTTAG	149.31154
BB93629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25774	WGS84	BB93629	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93629_Msal_Bot2012_COI	YD69	1	BB93629_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93629	Sample	BB93629	targeted	Absent	Sample	Msal_Bot2012_COI	YD69	COI	45	BB93629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD69	GYTAGTTCCRATGATGTTAGCTG	149.31154
BB93630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2553	WGS84	BB93630	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93630_Aamu_Bax2006_COI	YD65	1	BB93630_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93630	Sample	BB93630	targeted	Absent	Sample	Aamu_Bax2006_COI	YD65	COI	45	BB93630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD65	GCACAACCGGGATCTTTACTTC	149.31084
BB93630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2553	WGS84	BB93630	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93630_Pcan_Bot2011_IGS	YD65	1	BB93630_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93630	Sample	BB93630	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD65	IGS	45	BB93630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD65	CGTAATCCTCAGTACTGGCTA	149.31084
BB93630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2553	WGS84	BB93630	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93630_Sspa_Oph2007_COI	YD65	1	BB93630_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93630	Sample	BB93630	targeted	Absent	Sample	Sspa_Oph2007_COI	YD65	COI	45	BB93630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD65	GAAGGCCTTCCGATGATC	149.31084
BB93630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2553	WGS84	BB93630	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93630_Upin_Bot2011_COI	YD65	1	BB93630_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93630	Sample	BB93630	targeted	Absent	Sample	Upin_Bot2011_COI	YD65	COI	45	BB93630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD65	CTTTAATTACAGCGTTTTTATTGTTGT	149.31084
BB93631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25608	WGS84	BB93631	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93631_Mgig_Bot2012_COI	YD64	1	BB93631_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93631	Sample	BB93631	targeted	Absent	Sample	Mgig_Bot2012_COI	YD64	COI	45	BB93631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD64	TCTTATTCGTTGGAGACTTTATAACCCT	149.31047
BB93632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25453	WGS84	BB93632	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93632_Cmae_Bot2011_COI	YD63	1	BB93632_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93632	Sample	BB93632	targeted	Absent	Sample	Cmae_Bot2011_COI	YD63	COI	45	BB93632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD63	ATGAACAGTCTATCCTCCTTTAG	149.31127
BB93632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25453	WGS84	BB93632	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93632_Msal_Bot2012_COI	YD63	1	BB93632_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93632	Sample	BB93632	targeted	Absent	Sample	Msal_Bot2012_COI	YD63	COI	45	BB93632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD63	GYTAGTTCCRATGATGTTAGCTG	149.31127
BB93634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25389	WGS84	BB93634	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93634_Aamu_Bax2006_COI	YD59	1	BB93634_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93634	Sample	BB93634	targeted	Absent	Sample	Aamu_Bax2006_COI	YD59	COI	45	BB93634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD59	GCACAACCGGGATCTTTACTTC	149.31064
BB93634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25389	WGS84	BB93634	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93634_Pcan_Bot2011_IGS	YD59	1	BB93634_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93634	Sample	BB93634	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD59	IGS	45	BB93634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD59	CGTAATCCTCAGTACTGGCTA	149.31064
BB93634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25389	WGS84	BB93634	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93634_Sspa_Oph2007_COI	YD59	1	BB93634_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93634	Sample	BB93634	targeted	Absent	Sample	Sspa_Oph2007_COI	YD59	COI	45	BB93634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD59	GAAGGCCTTCCGATGATC	149.31064
BB93634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25389	WGS84	BB93634	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93634_Upin_Bot2011_COI	YD59	1	BB93634_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93634	Sample	BB93634	targeted	Absent	Sample	Upin_Bot2011_COI	YD59	COI	45	BB93634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD59	CTTTAATTACAGCGTTTTTATTGTTGT	149.31064
BB93635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25324	WGS84	BB93635	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93635_Mgig_Bot2012_COI	YD55	1	BB93635_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93635	Sample	BB93635	targeted	Absent	Sample	Mgig_Bot2012_COI	YD55	COI	45	BB93635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD55	TCTTATTCGTTGGAGACTTTATAACCCT	149.30899
BB93636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25376	WGS84	BB93636	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93636_Cmae_Bot2011_COI	YD54	1	BB93636_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93636	Sample	BB93636	targeted	Absent	Sample	Cmae_Bot2011_COI	YD54	COI	45	BB93636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD54	ATGAACAGTCTATCCTCCTTTAG	149.30868
BB93636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25376	WGS84	BB93636	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93636_Msal_Bot2012_COI	YD54	1	BB93636_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93636	Sample	BB93636	targeted	Absent	Sample	Msal_Bot2012_COI	YD54	COI	45	BB93636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD54	GYTAGTTCCRATGATGTTAGCTG	149.30868
BB93638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25162	WGS84	BB93638	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93638_Aamu_Bax2006_COI	YD51	1	BB93638_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93638	Sample	BB93638	targeted	Absent	Sample	Aamu_Bax2006_COI	YD51	COI	45	BB93638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD51	GCACAACCGGGATCTTTACTTC	149.3087
BB93638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25162	WGS84	BB93638	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93638_Pcan_Bot2011_IGS	YD51	1	BB93638_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93638	Sample	BB93638	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD51	IGS	45	BB93638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD51	CGTAATCCTCAGTACTGGCTA	149.3087
BB93638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25162	WGS84	BB93638	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93638_Sspa_Oph2007_COI	YD51	1	BB93638_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93638	Sample	BB93638	targeted	Absent	Sample	Sspa_Oph2007_COI	YD51	COI	45	BB93638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD51	GAAGGCCTTCCGATGATC	149.3087
BB93638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25162	WGS84	BB93638	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93638_Upin_Bot2011_COI	YD51	1	BB93638_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93638	Sample	BB93638	targeted	Absent	Sample	Upin_Bot2011_COI	YD51	COI	45	BB93638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD51	CTTTAATTACAGCGTTTTTATTGTTGT	149.3087
BB93639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24849	WGS84	BB93639	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93639_Mgig_Bot2012_COI	YD47	1	BB93639_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93639	Sample	BB93639	targeted	Absent	Sample	Mgig_Bot2012_COI	YD47	COI	45	BB93639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD47	TCTTATTCGTTGGAGACTTTATAACCCT	149.30523
BB93641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24705	WGS84	BB93641	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93641_Aamu_Bax2006_COI	YD44	1	BB93641_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93641	Sample	BB93641	targeted	Absent	Sample	Aamu_Bax2006_COI	YD44	COI	45	BB93641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD44	GCACAACCGGGATCTTTACTTC	149.30487
BB93641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24705	WGS84	BB93641	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93641_Pcan_Bot2011_IGS	YD44	1	BB93641_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93641	Sample	BB93641	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD44	IGS	45	BB93641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD44	CGTAATCCTCAGTACTGGCTA	149.30487
BB93641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24705	WGS84	BB93641	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93641_Sspa_Oph2007_COI	YD44	1	BB93641_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93641	Sample	BB93641	targeted	Absent	Sample	Sspa_Oph2007_COI	YD44	COI	45	BB93641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD44	GAAGGCCTTCCGATGATC	149.30487
BB93641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24705	WGS84	BB93641	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93641_Upin_Bot2011_COI	YD44	1	BB93641_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93641	Sample	BB93641	targeted	Absent	Sample	Upin_Bot2011_COI	YD44	COI	45	BB93641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD44	CTTTAATTACAGCGTTTTTATTGTTGT	149.30487
BB93642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2454	WGS84	BB93642	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93642_Mgig_Bot2012_COI	YD42	1	BB93642_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93642	Sample	BB93642	targeted	Absent	Sample	Mgig_Bot2012_COI	YD42	COI	45	BB93642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD42	TCTTATTCGTTGGAGACTTTATAACCCT	149.30471
BB93643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24635	WGS84	BB93643	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93643_Cmae_Bot2011_COI	YD41	1	BB93643_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93643	Sample	BB93643	targeted	Absent	Sample	Cmae_Bot2011_COI	YD41	COI	45	BB93643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD41	ATGAACAGTCTATCCTCCTTTAG	149.30463
BB93643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24635	WGS84	BB93643	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93643_Msal_Bot2012_COI	YD41	1	BB93643_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93643	Sample	BB93643	targeted	Absent	Sample	Msal_Bot2012_COI	YD41	COI	45	BB93643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD41	GYTAGTTCCRATGATGTTAGCTG	149.30463
BB93645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24561	WGS84	BB93645	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93645_Aamu_Bax2006_COI	YD38	1	BB93645_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93645	Sample	BB93645	targeted	Absent	Sample	Aamu_Bax2006_COI	YD38	COI	45	BB93645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD38	GCACAACCGGGATCTTTACTTC	149.30415
BB93645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24561	WGS84	BB93645	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93645_Pcan_Bot2011_IGS	YD38	1	BB93645_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93645	Sample	BB93645	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD38	IGS	45	BB93645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD38	CGTAATCCTCAGTACTGGCTA	149.30415
BB93645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24561	WGS84	BB93645	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93645_Sspa_Oph2007_COI	YD38	1	BB93645_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93645	Sample	BB93645	targeted	Absent	Sample	Sspa_Oph2007_COI	YD38	COI	45	BB93645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD38	GAAGGCCTTCCGATGATC	149.30415
BB93645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24561	WGS84	BB93645	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93645_Upin_Bot2011_COI	YD38	1	BB93645_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93645	Sample	BB93645	targeted	Absent	Sample	Upin_Bot2011_COI	YD38	COI	45	BB93645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD38	CTTTAATTACAGCGTTTTTATTGTTGT	149.30415
BB93646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24498	WGS84	BB93646	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93646_Mgig_Bot2012_COI	YD36	1	BB93646_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93646	Sample	BB93646	targeted	Absent	Sample	Mgig_Bot2012_COI	YD36	COI	45	BB93646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD36	TCTTATTCGTTGGAGACTTTATAACCCT	149.30349
BB93647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24356	WGS84	BB93647	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93647_Cmae_Bot2011_COI	YD35	1	BB93647_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93647	Sample	BB93647	targeted	Absent	Sample	Cmae_Bot2011_COI	YD35	COI	45	BB93647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD35	ATGAACAGTCTATCCTCCTTTAG	149.30339
BB93647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24356	WGS84	BB93647	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93647_Msal_Bot2012_COI	YD35	1	BB93647_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93647	Sample	BB93647	targeted	Absent	Sample	Msal_Bot2012_COI	YD35	COI	45	BB93647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD35	GYTAGTTCCRATGATGTTAGCTG	149.30339
BB93649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25118	WGS84	BB93649	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93649_Aamu_Bax2006_COI	YD26	1	BB93649_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93649	Sample	BB93649	targeted	Absent	Sample	Aamu_Bax2006_COI	YD26	COI	45	BB93649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD26	GCACAACCGGGATCTTTACTTC	149.30754
BB93649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25118	WGS84	BB93649	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93649_Pcan_Bot2011_IGS	YD26	1	BB93649_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93649	Sample	BB93649	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD26	IGS	45	BB93649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD26	CGTAATCCTCAGTACTGGCTA	149.30754
BB93649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25118	WGS84	BB93649	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93649_Sspa_Oph2007_COI	YD26	1	BB93649_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93649	Sample	BB93649	targeted	Absent	Sample	Sspa_Oph2007_COI	YD26	COI	45	BB93649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD26	GAAGGCCTTCCGATGATC	149.30754
BB93649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25118	WGS84	BB93649	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93649_Upin_Bot2011_COI	YD26	1	BB93649_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93649	Sample	BB93649	targeted	Absent	Sample	Upin_Bot2011_COI	YD26	COI	45	BB93649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD26	CTTTAATTACAGCGTTTTTATTGTTGT	149.30754
BB93650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24972	WGS84	BB93650	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93650_Cmae_Bot2011_COI	YD24	1	BB93650_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93650	Sample	BB93650	targeted	Absent	Sample	Cmae_Bot2011_COI	YD24	COI	45	BB93650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD24	ATGAACAGTCTATCCTCCTTTAG	149.30738
BB93650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24972	WGS84	BB93650	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93650_Msal_Bot2012_COI	YD24	1	BB93650_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93650	Sample	BB93650	targeted	Absent	Sample	Msal_Bot2012_COI	YD24	COI	45	BB93650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD24	GYTAGTTCCRATGATGTTAGCTG	149.30738
BB93652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24989	WGS84	BB93652	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93652_Aamu_Bax2006_COI	YD20	1	BB93652_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93652	Sample	BB93652	targeted	Absent	Sample	Aamu_Bax2006_COI	YD20	COI	45	BB93652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD20	GCACAACCGGGATCTTTACTTC	149.30652
BB93652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24989	WGS84	BB93652	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93652_Pcan_Bot2011_IGS	YD20	1	BB93652_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93652	Sample	BB93652	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD20	IGS	45	BB93652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD20	CGTAATCCTCAGTACTGGCTA	149.30652
BB93652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24989	WGS84	BB93652	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93652_Sspa_Oph2007_COI	YD20	1	BB93652_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93652	Sample	BB93652	targeted	Absent	Sample	Sspa_Oph2007_COI	YD20	COI	45	BB93652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD20	GAAGGCCTTCCGATGATC	149.30652
BB93652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24989	WGS84	BB93652	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93652_Upin_Bot2011_COI	YD20	1	BB93652_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93652	Sample	BB93652	targeted	Absent	Sample	Upin_Bot2011_COI	YD20	COI	45	BB93652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD20	CTTTAATTACAGCGTTTTTATTGTTGT	149.30652
BB93653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24764	WGS84	BB93653	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93653_Mgig_Bot2012_COI	YD19	1	BB93653_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93653	Sample	BB93653	targeted	Absent	Sample	Mgig_Bot2012_COI	YD19	COI	45	BB93653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD19	TCTTATTCGTTGGAGACTTTATAACCCT	149.30671
BB93654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24845	WGS84	BB93654	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93654_Cmae_Bot2011_COI	YD18	1	BB93654_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93654	Sample	BB93654	targeted	Absent	Sample	Cmae_Bot2011_COI	YD18	COI	45	BB93654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD18	ATGAACAGTCTATCCTCCTTTAG	149.3065
BB93654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24845	WGS84	BB93654	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93654_Msal_Bot2012_COI	YD18	1	BB93654_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93654	Sample	BB93654	targeted	Absent	Sample	Msal_Bot2012_COI	YD18	COI	45	BB93654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD18	GYTAGTTCCRATGATGTTAGCTG	149.3065
BB93656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24951	WGS84	BB93656	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93656_Aamu_Bax2006_COI	YD15	1	BB93656_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93656	Sample	BB93656	targeted	Absent	Sample	Aamu_Bax2006_COI	YD15	COI	45	BB93656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD15	GCACAACCGGGATCTTTACTTC	149.30338
BB93656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24951	WGS84	BB93656	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93656_Pcan_Bot2011_IGS	YD15	1	BB93656_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93656	Sample	BB93656	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD15	IGS	45	BB93656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD15	CGTAATCCTCAGTACTGGCTA	149.30338
BB93656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24951	WGS84	BB93656	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93656_Sspa_Oph2007_COI	YD15	1	BB93656_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93656	Sample	BB93656	targeted	Absent	Sample	Sspa_Oph2007_COI	YD15	COI	45	BB93656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD15	GAAGGCCTTCCGATGATC	149.30338
BB93656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24951	WGS84	BB93656	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93656_Upin_Bot2011_COI	YD15	1	BB93656_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93656	Sample	BB93656	targeted	Absent	Sample	Upin_Bot2011_COI	YD15	COI	45	BB93656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD15	CTTTAATTACAGCGTTTTTATTGTTGT	149.30338
BB93657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24873	WGS84	BB93657	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93657_Mgig_Bot2012_COI	YD14	1	BB93657_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93657	Sample	BB93657	targeted	Absent	Sample	Mgig_Bot2012_COI	YD14	COI	45	BB93657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD14	TCTTATTCGTTGGAGACTTTATAACCCT	149.30351
BB93658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24742	WGS84	BB93658	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93658_Cmae_Bot2011_COI	YD12	1	BB93658_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93658	Sample	BB93658	targeted	Absent	Sample	Cmae_Bot2011_COI	YD12	COI	45	BB93658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD12	ATGAACAGTCTATCCTCCTTTAG	149.30258
BB93658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24742	WGS84	BB93658	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93658_Msal_Bot2012_COI	YD12	1	BB93658_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93658	Sample	BB93658	targeted	Absent	Sample	Msal_Bot2012_COI	YD12	COI	45	BB93658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD12	GYTAGTTCCRATGATGTTAGCTG	149.30258
BB93660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24476	WGS84	BB93660	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93660_Mgig_Bot2012_COI	YD8	1	BB93660_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93660	Sample	BB93660	targeted	Absent	Sample	Mgig_Bot2012_COI	YD8	COI	45	BB93660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD8	TCTTATTCGTTGGAGACTTTATAACCCT	149.30136
BB93661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2443	WGS84	BB93661	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93661_Cmae_Bot2011_COI	YD7	1	BB93661_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93661	Sample	BB93661	targeted	Absent	Sample	Cmae_Bot2011_COI	YD7	COI	45	BB93661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD7	ATGAACAGTCTATCCTCCTTTAG	149.30093
BB93661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2443	WGS84	BB93661	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93661_Msal_Bot2012_COI	YD7	1	BB93661_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93661	Sample	BB93661	targeted	Absent	Sample	Msal_Bot2012_COI	YD7	COI	45	BB93661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD7	GYTAGTTCCRATGATGTTAGCTG	149.30093
BB93663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24239	WGS84	BB93663	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93663_Aamu_Bax2006_COI	YD4	1	BB93663_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93663	Sample	BB93663	targeted	Absent	Sample	Aamu_Bax2006_COI	YD4	COI	45	BB93663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD4	GCACAACCGGGATCTTTACTTC	149.30341
BB93663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24239	WGS84	BB93663	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93663_Pcan_Bot2011_IGS	YD4	1	BB93663_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93663	Sample	BB93663	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD4	IGS	45	BB93663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD4	CGTAATCCTCAGTACTGGCTA	149.30341
BB93663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24239	WGS84	BB93663	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93663_Sspa_Oph2007_COI	YD4	1	BB93663_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93663	Sample	BB93663	targeted	Absent	Sample	Sspa_Oph2007_COI	YD4	COI	45	BB93663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD4	GAAGGCCTTCCGATGATC	149.30341
BB93663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24239	WGS84	BB93663	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93663_Upin_Bot2011_COI	YD4	1	BB93663_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93663	Sample	BB93663	targeted	Absent	Sample	Upin_Bot2011_COI	YD4	COI	45	BB93663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD4	CTTTAATTACAGCGTTTTTATTGTTGT	149.30341
BB93664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24177	WGS84	BB93664	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93664_Mgig_Bot2012_COI	YD2	1	BB93664_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93664	Sample	BB93664	targeted	Absent	Sample	Mgig_Bot2012_COI	YD2	COI	45	BB93664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD2	TCTTATTCGTTGGAGACTTTATAACCCT	149.30205
BB93665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24181	WGS84	BB93665	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93665_Cmae_Bot2011_COI	YD1	1	BB93665_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93665	Sample	BB93665	targeted	Absent	Sample	Cmae_Bot2011_COI	YD1	COI	45	BB93665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD1	ATGAACAGTCTATCCTCCTTTAG	149.30164
BB93665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24181	WGS84	BB93665	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93665_Msal_Bot2012_COI	YD1	1	BB93665_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93665	Sample	BB93665	targeted	Absent	Sample	Msal_Bot2012_COI	YD1	COI	45	BB93665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD1	GYTAGTTCCRATGATGTTAGCTG	149.30164
BB93667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18664	WGS84	BB93667	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93667_Aamu_Bax2006_COI	DD8	1	BB93667_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93667	Sample	BB93667	targeted	Absent	Sample	Aamu_Bax2006_COI	DD8	COI	45	BB93667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD8	GCACAACCGGGATCTTTACTTC	146.36605
BB93667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18664	WGS84	BB93667	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93667_Pcan_Bot2011_IGS	DD8	1	BB93667_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93667	Sample	BB93667	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD8	IGS	45	BB93667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD8	CGTAATCCTCAGTACTGGCTA	146.36605
BB93667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18664	WGS84	BB93667	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93667_Sspa_Oph2007_COI	DD8	1	BB93667_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93667	Sample	BB93667	targeted	Absent	Sample	Sspa_Oph2007_COI	DD8	COI	45	BB93667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD8	GAAGGCCTTCCGATGATC	146.36605
BB93667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18664	WGS84	BB93667	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93667_Upin_Bot2011_COI	DD8	1	BB93667_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93667	Sample	BB93667	targeted	Absent	Sample	Upin_Bot2011_COI	DD8	COI	45	BB93667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD8	CTTTAATTACAGCGTTTTTATTGTTGT	146.36605
BB93668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18778	WGS84	BB93668	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93668_Mgig_Bot2012_COI	DD6	1	BB93668_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93668	Sample	BB93668	targeted	Absent	Sample	Mgig_Bot2012_COI	DD6	COI	45	BB93668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD6	TCTTATTCGTTGGAGACTTTATAACCCT	146.36694
BB93669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.19181	WGS84	BB93669	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.863	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93669_Cmae_Bot2011_COI	DD1	1	BB93669_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93669	Sample	BB93669	targeted	Present	Sample	Cmae_Bot2011_COI	DD1	COI	45	BB93669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD1	ATGAACAGTCTATCCTCCTTTAG	146.36808
BB93669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.19181	WGS84	BB93669	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93669_Msal_Bot2012_COI	DD1	1	BB93669_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93669	Sample	BB93669	targeted	Absent	Sample	Msal_Bot2012_COI	DD1	COI	45	BB93669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD1	GYTAGTTCCRATGATGTTAGCTG	146.36808
BB93670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.1886	WGS84	BB93670	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93670_Aamu_Bax2006_COI	DD3	1	BB93670_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93670	Sample	BB93670	targeted	Absent	Sample	Aamu_Bax2006_COI	DD3	COI	45	BB93670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD3	GCACAACCGGGATCTTTACTTC	146.36678
BB93670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.1886	WGS84	BB93670	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93670_Pcan_Bot2011_IGS	DD3	1	BB93670_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93670	Sample	BB93670	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD3	IGS	45	BB93670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD3	CGTAATCCTCAGTACTGGCTA	146.36678
BB93670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.1886	WGS84	BB93670	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93670_Sspa_Oph2007_COI	DD3	1	BB93670_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93670	Sample	BB93670	targeted	Absent	Sample	Sspa_Oph2007_COI	DD3	COI	45	BB93670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD3	GAAGGCCTTCCGATGATC	146.36678
BB93670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.1886	WGS84	BB93670	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93670_Upin_Bot2011_COI	DD3	1	BB93670_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93670	Sample	BB93670	targeted	Absent	Sample	Upin_Bot2011_COI	DD3	COI	45	BB93670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD3	CTTTAATTACAGCGTTTTTATTGTTGT	146.36678
BB93671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18763	WGS84	BB93671	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.391	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93671_Mgig_Bot2012_COI	DD5	1	BB93671_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93671	Sample	BB93671	targeted	Present	Sample	Mgig_Bot2012_COI	DD5	COI	45	BB93671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD5	TCTTATTCGTTGGAGACTTTATAACCCT	146.36571
BB93672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93672	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93672_Cmae_Bot2011_COI	DD9	1	BB93672_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93672	Sample	BB93672	targeted	Absent	Sample	Cmae_Bot2011_COI	DD9	COI	45	BB93672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD9	ATGAACAGTCTATCCTCCTTTAG	146.36416
BB93672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93672	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93672_Msal_Bot2012_COI	DD9	1	BB93672_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93672	Sample	BB93672	targeted	Absent	Sample	Msal_Bot2012_COI	DD9	COI	45	BB93672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD9	GYTAGTTCCRATGATGTTAGCTG	146.36416
BB93674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18474	WGS84	BB93674	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93674_Pcan_Bot2011_IGS	DD12	1	BB93674_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93674	Sample	BB93674	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD12	IGS	45	BB93674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD12	CGTAATCCTCAGTACTGGCTA	146.36414
BB93674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18474	WGS84	BB93674	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93674_Sspa_Oph2007_COI	DD12	1	BB93674_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93674	Sample	BB93674	targeted	Absent	Sample	Sspa_Oph2007_COI	DD12	COI	45	BB93674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD12	GAAGGCCTTCCGATGATC	146.36414
BB93674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18474	WGS84	BB93674	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93674_Upin_Bot2011_COI	DD12	1	BB93674_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93674	Sample	BB93674	targeted	Absent	Sample	Upin_Bot2011_COI	DD12	COI	45	BB93674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD12	CTTTAATTACAGCGTTTTTATTGTTGT	146.36414
BB93675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18176	WGS84	BB93675	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93675_Mgig_Bot2012_COI	DD17	1	BB93675_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93675	Sample	BB93675	targeted	Absent	Sample	Mgig_Bot2012_COI	DD17	COI	45	BB93675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD17	TCTTATTCGTTGGAGACTTTATAACCCT	146.3652
BB93676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18283	WGS84	BB93676	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93676_Cmae_Bot2011_COI	DD15	1	BB93676_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93676	Sample	BB93676	targeted	Absent	Sample	Cmae_Bot2011_COI	DD15	COI	45	BB93676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD15	ATGAACAGTCTATCCTCCTTTAG	146.36596
BB93676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18283	WGS84	BB93676	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93676_Msal_Bot2012_COI	DD15	1	BB93676_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93676	Sample	BB93676	targeted	Absent	Sample	Msal_Bot2012_COI	DD15	COI	45	BB93676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD15	GYTAGTTCCRATGATGTTAGCTG	146.36596
BB93678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18188	WGS84	BB93678	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93678_Pcan_Bot2011_IGS	DD18	1	BB93678_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93678	Sample	BB93678	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD18	IGS	45	BB93678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD18	CGTAATCCTCAGTACTGGCTA	146.36437
BB93678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18188	WGS84	BB93678	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93678_Sspa_Oph2007_COI	DD18	1	BB93678_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93678	Sample	BB93678	targeted	Absent	Sample	Sspa_Oph2007_COI	DD18	COI	45	BB93678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD18	GAAGGCCTTCCGATGATC	146.36437
BB93678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18188	WGS84	BB93678	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93678_Upin_Bot2011_COI	DD18	1	BB93678_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93678	Sample	BB93678	targeted	Absent	Sample	Upin_Bot2011_COI	DD18	COI	45	BB93678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD18	CTTTAATTACAGCGTTTTTATTGTTGT	146.36437
BB93679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18084	WGS84	BB93679	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.303	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93679_Mgig_Bot2012_COI	DD19	1	BB93679_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93679	Sample	BB93679	targeted	Present	Sample	Mgig_Bot2012_COI	DD19	COI	45	BB93679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD19	TCTTATTCGTTGGAGACTTTATAACCCT	146.36514
BB93681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8857	WGS84	BB93681	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.435	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93681_Aamu_Bax2006_COI	HD21	1	BB93681_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93681	Sample	BB93681	targeted	Present	Sample	Aamu_Bax2006_COI	HD21	COI	45	BB93681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD21	GCACAACCGGGATCTTTACTTC	147.33873
BB93681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8857	WGS84	BB93681	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93681_Pcan_Bot2011_IGS	HD21	1	BB93681_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93681	Sample	BB93681	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD21	IGS	45	BB93681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD21	CGTAATCCTCAGTACTGGCTA	147.33873
BB93681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8857	WGS84	BB93681	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93681_Sspa_Oph2007_COI	HD21	1	BB93681_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93681	Sample	BB93681	targeted	Absent	Sample	Sspa_Oph2007_COI	HD21	COI	45	BB93681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD21	GAAGGCCTTCCGATGATC	147.33873
BB93681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8857	WGS84	BB93681	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93681_Upin_Bot2011_COI	HD21	1	BB93681_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93681	Sample	BB93681	targeted	Absent	Sample	Upin_Bot2011_COI	HD21	COI	45	BB93681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD21	CTTTAATTACAGCGTTTTTATTGTTGT	147.33873
BB93682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88291	WGS84	BB93682	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93682_Mgig_Bot2012_COI	HD19	1	BB93682_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93682	Sample	BB93682	targeted	Absent	Sample	Mgig_Bot2012_COI	HD19	COI	45	BB93682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD19	TCTTATTCGTTGGAGACTTTATAACCCT	147.33838
BB93683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84434	WGS84	BB93683	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.727	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93683_Cmae_Bot2011_COI	HD6	1	BB93683_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93683	Sample	BB93683	targeted	Present	Sample	Cmae_Bot2011_COI	HD6	COI	45	BB93683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD6	ATGAACAGTCTATCCTCCTTTAG	147.32872
BB93683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84434	WGS84	BB93683	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93683_Msal_Bot2012_COI	HD6	1	BB93683_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93683	Sample	BB93683	targeted	Absent	Sample	Msal_Bot2012_COI	HD6	COI	45	BB93683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD6	GYTAGTTCCRATGATGTTAGCTG	147.32872
BB93685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84362	WGS84	BB93685	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.187	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93685_Aamu_Bax2006_COI	HD8	1	BB93685_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93685	Sample	BB93685	targeted	Present	Sample	Aamu_Bax2006_COI	HD8	COI	45	BB93685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD8	GCACAACCGGGATCTTTACTTC	147.32459
BB93685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84362	WGS84	BB93685	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93685_Pcan_Bot2011_IGS	HD8	1	BB93685_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93685	Sample	BB93685	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD8	IGS	45	BB93685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD8	CGTAATCCTCAGTACTGGCTA	147.32459
BB93685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84362	WGS84	BB93685	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93685_Sspa_Oph2007_COI	HD8	1	BB93685_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93685	Sample	BB93685	targeted	Absent	Sample	Sspa_Oph2007_COI	HD8	COI	45	BB93685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD8	GAAGGCCTTCCGATGATC	147.32459
BB93685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84362	WGS84	BB93685	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93685_Upin_Bot2011_COI	HD8	1	BB93685_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93685	Sample	BB93685	targeted	Absent	Sample	Upin_Bot2011_COI	HD8	COI	45	BB93685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD8	CTTTAATTACAGCGTTTTTATTGTTGT	147.32459
BB93686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87278	WGS84	BB93686	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93686_Mgig_Bot2012_COI	HD9	1	BB93686_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93686	Sample	BB93686	targeted	Absent	Sample	Mgig_Bot2012_COI	HD9	COI	45	BB93686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD9	TCTTATTCGTTGGAGACTTTATAACCCT	147.33622
BB93687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87576	WGS84	BB93687	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	39.805	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93687_Cmae_Bot2011_COI	HD11	1	BB93687_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93687	Sample	BB93687	targeted	Present	Sample	Cmae_Bot2011_COI	HD11	COI	45	BB93687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD11	ATGAACAGTCTATCCTCCTTTAG	147.33896
BB93687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87576	WGS84	BB93687	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93687_Msal_Bot2012_COI	HD11	1	BB93687_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93687	Sample	BB93687	targeted	Absent	Sample	Msal_Bot2012_COI	HD11	COI	45	BB93687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD11	GYTAGTTCCRATGATGTTAGCTG	147.33896
BB93689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87848	WGS84	BB93689	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.683	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93689_Aamu_Bax2006_COI	HD14	1	BB93689_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93689	Sample	BB93689	targeted	Present	Sample	Aamu_Bax2006_COI	HD14	COI	45	BB93689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD14	GCACAACCGGGATCTTTACTTC	147.34167
BB93689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87848	WGS84	BB93689	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93689_Pcan_Bot2011_IGS	HD14	1	BB93689_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93689	Sample	BB93689	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD14	IGS	45	BB93689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD14	CGTAATCCTCAGTACTGGCTA	147.34167
BB93689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87848	WGS84	BB93689	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93689_Sspa_Oph2007_COI	HD14	1	BB93689_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93689	Sample	BB93689	targeted	Absent	Sample	Sspa_Oph2007_COI	HD14	COI	45	BB93689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD14	GAAGGCCTTCCGATGATC	147.34167
BB93689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87848	WGS84	BB93689	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93689_Upin_Bot2011_COI	HD14	1	BB93689_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93689	Sample	BB93689	targeted	Absent	Sample	Upin_Bot2011_COI	HD14	COI	45	BB93689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD14	CTTTAATTACAGCGTTTTTATTGTTGT	147.34167
BB93690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88172	WGS84	BB93690	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93690_Cmae_Bot2011_COI	HD16	1	BB93690_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93690	Sample	BB93690	targeted	Absent	Sample	Cmae_Bot2011_COI	HD16	COI	45	BB93690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD16	ATGAACAGTCTATCCTCCTTTAG	147.34203
BB93690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88172	WGS84	BB93690	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93690_Msal_Bot2012_COI	HD16	1	BB93690_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93690	Sample	BB93690	targeted	Absent	Sample	Msal_Bot2012_COI	HD16	COI	45	BB93690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD16	GYTAGTTCCRATGATGTTAGCTG	147.34203
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93692_Aamu_Bax2006_COI	VD20	1	BB93692_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Aamu_Bax2006_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	GCACAACCGGGATCTTTACTTC	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93692_Pcan_Bot2011_IGS	VD20	1	BB93692_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD20	IGS	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	CGTAATCCTCAGTACTGGCTA	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93692_Pper_Dia2013_COI	VD20	1	BB93692_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Pper_Dia2013_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	CTTAGTGGCATTAATTCGDAATCC	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93692_Sspa_Oph2007_COI	VD20	1	BB93692_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Sspa_Oph2007_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	GAAGGCCTTCCGATGATC	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93692_Upin_Bot2011_COI	VD20	1	BB93692_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Upin_Bot2011_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	CTTTAATTACAGCGTTTTTATTGTTGT	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20489	WGS84	BB93692	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93692_Vgib_Wil2025_COI	VD20	1	BB93692_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Vgib_Wil2025_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	CCCCTCTTTACAAGTTCTTTAGGGT	136.68229
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20575	WGS84	BB93693	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93693_Asen_Wil2025_COI	VD10	1	BB93693_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Asen_Wil2025_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	GTTGTTGTTACAACACATGCTCTTTTG	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93693_Cjap_Sim2018_COI	VD10	1	BB93693_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Cjap_Sim2018_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93693_Esin_And2018_Cyb	VD10	1	BB93693_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Esin_And2018_Cyb	VD10	Cytb	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	ACCCCTCCTCATATCCAACCA	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93693_Mgig_Bot2012_COI	VD10	1	BB93693_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Mgig_Bot2012_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	TCTTATTCGTTGGAGACTTTATAACCCT	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93693_Mjap_Gib2022_COI	VD10	1	BB93693_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Mjap_Gib2022_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	AATTTAATTGTGACTAGGCATGGTCTG	136.66871
BB93694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19539	WGS84	BB93694	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93694_Cmae_Bot2011_COI	VD1	1	BB93694_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93694	Sample	BB93694	targeted	Absent	Sample	Cmae_Bot2011_COI	VD1	COI	45	BB93694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD1	ATGAACAGTCTATCCTCCTTTAG	136.68657
BB93694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19539	WGS84	BB93694	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93694_Msal_Bot2012_COI	VD1	1	BB93694_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93694	Sample	BB93694	targeted	Absent	Sample	Msal_Bot2012_COI	VD1	COI	45	BB93694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD1	GYTAGTTCCRATGATGTTAGCTG	136.68657
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93696_Aamu_Bax2006_COI	VD3	1	BB93696_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Aamu_Bax2006_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	GCACAACCGGGATCTTTACTTC	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93696_Pcan_Bot2011_IGS	VD3	1	BB93696_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD3	IGS	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	CGTAATCCTCAGTACTGGCTA	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93696_Pper_Dia2013_COI	VD3	1	BB93696_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Pper_Dia2013_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	CTTAGTGGCATTAATTCGDAATCC	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93696_Sspa_Oph2007_COI	VD3	1	BB93696_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Sspa_Oph2007_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	GAAGGCCTTCCGATGATC	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93696_Upin_Bot2011_COI	VD3	1	BB93696_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Upin_Bot2011_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	CTTTAATTACAGCGTTTTTATTGTTGT	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.2061	WGS84	BB93696	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93696_Vgib_Wil2025_COI	VD3	1	BB93696_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Vgib_Wil2025_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	CCCCTCTTTACAAGTTCTTTAGGGT	136.68091
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20645	WGS84	BB93697	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93697_Asen_Wil2025_COI	VD4	1	BB93697_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Asen_Wil2025_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	GTTGTTGTTACAACACATGCTCTTTTG	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93697_Cjap_Sim2018_COI	VD4	1	BB93697_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Cjap_Sim2018_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93697_Esin_And2018_Cyb	VD4	1	BB93697_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Esin_And2018_Cyb	VD4	Cytb	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	ACCCCTCCTCATATCCAACCA	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93697_Mgig_Bot2012_COI	VD4	1	BB93697_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Mgig_Bot2012_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	TCTTATTCGTTGGAGACTTTATAACCCT	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93697_Mjap_Gib2022_COI	VD4	1	BB93697_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Mjap_Gib2022_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	AATTTAATTGTGACTAGGCATGGTCTG	136.67967
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93698_Cmae_Bot2011_COI	VD18	1	BB93698_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Cmae_Bot2011_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	ATGAACAGTCTATCCTCCTTTAG	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93698_Hsan_Wil2021_COI	VD18	1	BB93698_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Hsan_Wil2021_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	CCTGGGCCGGTATAGTAGGT	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93698_Mros_Gib2022_COI	VD18	1	BB93698_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Mros_Gib2022_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93698_Msal_Bot2012_COI	VD18	1	BB93698_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Msal_Bot2012_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	GYTAGTTCCRATGATGTTAGCTG	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93698_Pvir_Dia2013_COI	VD18	1	BB93698_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Pvir_Dia2013_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	CTTAGTGGCATTAATTCGDAATCC	136.66947
BB93698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20913	WGS84	BB93698	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93698_Rhar_Sim2018_COI	VD18	1	BB93698_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93698	Sample	BB93698	targeted	Absent	Sample	Rhar_Sim2018_COI	VD18	COI	45	BB93698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD18	CTGCTGCTATTGCTCATG	136.66947
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93700_Aamu_Bax2006_COI	VD14	1	BB93700_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Aamu_Bax2006_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	GCACAACCGGGATCTTTACTTC	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93700_Pcan_Bot2011_IGS	VD14	1	BB93700_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD14	IGS	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	CGTAATCCTCAGTACTGGCTA	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93700_Pper_Dia2013_COI	VD14	1	BB93700_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Pper_Dia2013_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	CTTAGTGGCATTAATTCGDAATCC	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93700_Sspa_Oph2007_COI	VD14	1	BB93700_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Sspa_Oph2007_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	GAAGGCCTTCCGATGATC	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93700_Upin_Bot2011_COI	VD14	1	BB93700_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Upin_Bot2011_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	CTTTAATTACAGCGTTTTTATTGTTGT	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.21016	WGS84	BB93700	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93700_Vgib_Wil2025_COI	VD14	1	BB93700_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Vgib_Wil2025_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	CCCCTCTTTACAAGTTCTTTAGGGT	136.67151
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20918	WGS84	BB93701	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93701_Asen_Wil2025_COI	VD13	1	BB93701_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Asen_Wil2025_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	GTTGTTGTTACAACACATGCTCTTTTG	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93701_Cjap_Sim2018_COI	VD13	1	BB93701_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Cjap_Sim2018_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93701_Esin_And2018_Cyb	VD13	1	BB93701_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Esin_And2018_Cyb	VD13	Cytb	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	ACCCCTCCTCATATCCAACCA	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93701_Mgig_Bot2012_COI	VD13	1	BB93701_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Mgig_Bot2012_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	TCTTATTCGTTGGAGACTTTATAACCCT	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93701_Mjap_Gib2022_COI	VD13	1	BB93701_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Mjap_Gib2022_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	AATTTAATTGTGACTAGGCATGGTCTG	136.67057
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93702_Cmae_Bot2011_COI	VD12	1	BB93702_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Cmae_Bot2011_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	ATGAACAGTCTATCCTCCTTTAG	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93702_Hsan_Wil2021_COI	VD12	1	BB93702_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Hsan_Wil2021_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	CCTGGGCCGGTATAGTAGGT	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93702_Mros_Gib2022_COI	VD12	1	BB93702_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Mros_Gib2022_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93702_Msal_Bot2012_COI	VD12	1	BB93702_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Msal_Bot2012_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	GYTAGTTCCRATGATGTTAGCTG	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93702_Pvir_Dia2013_COI	VD12	1	BB93702_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Pvir_Dia2013_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	CTTAGTGGCATTAATTCGDAATCC	136.6698
BB93702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20771	WGS84	BB93702	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93702_Rhar_Sim2018_COI	VD12	1	BB93702_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93702	Sample	BB93702	targeted	Absent	Sample	Rhar_Sim2018_COI	VD12	COI	45	BB93702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD12	CTGCTGCTATTGCTCATG	136.6698
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93704_Aamu_Bax2006_COI	VD7	1	BB93704_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Aamu_Bax2006_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	GCACAACCGGGATCTTTACTTC	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93704_Pcan_Bot2011_IGS	VD7	1	BB93704_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD7	IGS	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	CGTAATCCTCAGTACTGGCTA	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93704_Pper_Dia2013_COI	VD7	1	BB93704_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Pper_Dia2013_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	CTTAGTGGCATTAATTCGDAATCC	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93704_Sspa_Oph2007_COI	VD7	1	BB93704_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Sspa_Oph2007_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	GAAGGCCTTCCGATGATC	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93704_Upin_Bot2011_COI	VD7	1	BB93704_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Upin_Bot2011_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	CTTTAATTACAGCGTTTTTATTGTTGT	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.204	WGS84	BB93704	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93704_Vgib_Wil2025_COI	VD7	1	BB93704_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Vgib_Wil2025_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	CCCCTCTTTACAAGTTCTTTAGGGT	136.67046
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20707	WGS84	BB93705	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93705_Asen_Wil2025_COI	VD6	1	BB93705_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Asen_Wil2025_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	GTTGTTGTTACAACACATGCTCTTTTG	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93705_Cjap_Sim2018_COI	VD6	1	BB93705_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Cjap_Sim2018_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93705_Esin_And2018_Cyb	VD6	1	BB93705_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Esin_And2018_Cyb	VD6	Cytb	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	ACCCCTCCTCATATCCAACCA	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93705_Mgig_Bot2012_COI	VD6	1	BB93705_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Mgig_Bot2012_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	TCTTATTCGTTGGAGACTTTATAACCCT	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93705_Mjap_Gib2022_COI	VD6	1	BB93705_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Mjap_Gib2022_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	AATTTAATTGTGACTAGGCATGGTCTG	136.67185
BB93706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84552	WGS84	BB93706	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.738	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93706_Cmae_Bot2011_COI	HD5	1	BB93706_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93706	Sample	BB93706	targeted	Present	Sample	Cmae_Bot2011_COI	HD5	COI	45	BB93706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD5	ATGAACAGTCTATCCTCCTTTAG	147.33115
BB93706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84552	WGS84	BB93706	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93706_Msal_Bot2012_COI	HD5	1	BB93706_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93706	Sample	BB93706	targeted	Absent	Sample	Msal_Bot2012_COI	HD5	COI	45	BB93706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD5	GYTAGTTCCRATGATGTTAGCTG	147.33115
BB93708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82814	WGS84	BB93708	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.655	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93708_Aamu_Bax2006_COI	HD3	1	BB93708_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93708	Sample	BB93708	targeted	Present	Sample	Aamu_Bax2006_COI	HD3	COI	45	BB93708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD3	GCACAACCGGGATCTTTACTTC	147.31543
BB93708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82814	WGS84	BB93708	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93708_Pcan_Bot2011_IGS	HD3	1	BB93708_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93708	Sample	BB93708	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD3	IGS	45	BB93708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD3	CGTAATCCTCAGTACTGGCTA	147.31543
BB93708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82814	WGS84	BB93708	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93708_Sspa_Oph2007_COI	HD3	1	BB93708_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93708	Sample	BB93708	targeted	Absent	Sample	Sspa_Oph2007_COI	HD3	COI	45	BB93708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD3	GAAGGCCTTCCGATGATC	147.31543
BB93708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82814	WGS84	BB93708	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93708_Upin_Bot2011_COI	HD3	1	BB93708_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93708	Sample	BB93708	targeted	Absent	Sample	Upin_Bot2011_COI	HD3	COI	45	BB93708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD3	CTTTAATTACAGCGTTTTTATTGTTGT	147.31543
BB93709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82857	WGS84	BB93709	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93709_Mgig_Bot2012_COI	HD2	1	BB93709_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93709	Sample	BB93709	targeted	Absent	Sample	Mgig_Bot2012_COI	HD2	COI	45	BB93709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD2	TCTTATTCGTTGGAGACTTTATAACCCT	147.3172
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93711_Aamu_Bax2006_COI	WD1	1	BB93711_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Aamu_Bax2006_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	GCACAACCGGGATCTTTACTTC	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93711_Pcan_Bot2011_IGS	WD1	1	BB93711_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD1	IGS	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	CGTAATCCTCAGTACTGGCTA	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93711_Pper_Dia2013_COI	WD1	1	BB93711_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Pper_Dia2013_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	CTTAGTGGCATTAATTCGDAATCC	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93711_Sspa_Oph2007_COI	WD1	1	BB93711_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Sspa_Oph2007_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	GAAGGCCTTCCGATGATC	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93711_Upin_Bot2011_COI	WD1	1	BB93711_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Upin_Bot2011_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	CTTTAATTACAGCGTTTTTATTGTTGT	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66717	WGS84	BB93711	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93711_Vgib_Wil2025_COI	WD1	1	BB93711_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Vgib_Wil2025_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	CCCCTCTTTACAAGTTCTTTAGGGT	141.84674
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66714	WGS84	BB93712	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93712_Asen_Wil2025_COI	WD3	1	BB93712_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Asen_Wil2025_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	GTTGTTGTTACAACACATGCTCTTTTG	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93712_Cjap_Sim2018_COI	WD3	1	BB93712_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Cjap_Sim2018_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93712_Esin_And2018_Cyb	WD3	1	BB93712_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Esin_And2018_Cyb	WD3	Cytb	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	ACCCCTCCTCATATCCAACCA	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93712_Mgig_Bot2012_COI	WD3	1	BB93712_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Mgig_Bot2012_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	TCTTATTCGTTGGAGACTTTATAACCCT	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93712_Mjap_Gib2022_COI	WD3	1	BB93712_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Mjap_Gib2022_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	AATTTAATTGTGACTAGGCATGGTCTG	141.84936
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93713_Cmae_Bot2011_COI	WD4	1	BB93713_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Cmae_Bot2011_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	ATGAACAGTCTATCCTCCTTTAG	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93713_Hsan_Wil2021_COI	WD4	1	BB93713_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Hsan_Wil2021_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	CCTGGGCCGGTATAGTAGGT	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93713_Mros_Gib2022_COI	WD4	1	BB93713_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Mros_Gib2022_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93713_Msal_Bot2012_COI	WD4	1	BB93713_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Msal_Bot2012_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	GYTAGTTCCRATGATGTTAGCTG	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93713_Pvir_Dia2013_COI	WD4	1	BB93713_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Pvir_Dia2013_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	CTTAGTGGCATTAATTCGDAATCC	141.84863
BB93713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66603	WGS84	BB93713	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93713_Rhar_Sim2018_COI	WD4	1	BB93713_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93713	Sample	BB93713	targeted	Absent	Sample	Rhar_Sim2018_COI	WD4	COI	45	BB93713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD4	CTGCTGCTATTGCTCATG	141.84863
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93715_Aamu_Bax2006_COI	WD8	1	BB93715_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Aamu_Bax2006_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	GCACAACCGGGATCTTTACTTC	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93715_Pcan_Bot2011_IGS	WD8	1	BB93715_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD8	IGS	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	CGTAATCCTCAGTACTGGCTA	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93715_Pper_Dia2013_COI	WD8	1	BB93715_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Pper_Dia2013_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	CTTAGTGGCATTAATTCGDAATCC	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93715_Sspa_Oph2007_COI	WD8	1	BB93715_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Sspa_Oph2007_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	GAAGGCCTTCCGATGATC	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93715_Upin_Bot2011_COI	WD8	1	BB93715_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Upin_Bot2011_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	CTTTAATTACAGCGTTTTTATTGTTGT	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66989	WGS84	BB93715	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93715_Vgib_Wil2025_COI	WD8	1	BB93715_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Vgib_Wil2025_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	CCCCTCTTTACAAGTTCTTTAGGGT	141.86241
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66985	WGS84	BB93716	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93716_Asen_Wil2025_COI	WD9	1	BB93716_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Asen_Wil2025_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	GTTGTTGTTACAACACATGCTCTTTTG	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93716_Cjap_Sim2018_COI	WD9	1	BB93716_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Cjap_Sim2018_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93716_Esin_And2018_Cyb	WD9	1	BB93716_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Esin_And2018_Cyb	WD9	Cytb	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	ACCCCTCCTCATATCCAACCA	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93716_Mgig_Bot2012_COI	WD9	1	BB93716_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Mgig_Bot2012_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	TCTTATTCGTTGGAGACTTTATAACCCT	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93716_Mjap_Gib2022_COI	WD9	1	BB93716_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Mjap_Gib2022_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	AATTTAATTGTGACTAGGCATGGTCTG	141.86396
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93717_Cmae_Bot2011_COI	WD11	1	BB93717_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Cmae_Bot2011_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	ATGAACAGTCTATCCTCCTTTAG	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93717_Hsan_Wil2021_COI	WD11	1	BB93717_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Hsan_Wil2021_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	CCTGGGCCGGTATAGTAGGT	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93717_Mros_Gib2022_COI	WD11	1	BB93717_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Mros_Gib2022_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93717_Msal_Bot2012_COI	WD11	1	BB93717_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Msal_Bot2012_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	GYTAGTTCCRATGATGTTAGCTG	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93717_Pvir_Dia2013_COI	WD11	1	BB93717_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Pvir_Dia2013_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	CTTAGTGGCATTAATTCGDAATCC	141.86743
BB93717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67253	WGS84	BB93717	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93717_Rhar_Sim2018_COI	WD11	1	BB93717_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93717	Sample	BB93717	targeted	Absent	Sample	Rhar_Sim2018_COI	WD11	COI	45	BB93717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD11	CTGCTGCTATTGCTCATG	141.86743
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93719_Aamu_Bax2006_COI	WD14	1	BB93719_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Aamu_Bax2006_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	GCACAACCGGGATCTTTACTTC	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93719_Pcan_Bot2011_IGS	WD14	1	BB93719_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD14	IGS	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	CGTAATCCTCAGTACTGGCTA	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93719_Pper_Dia2013_COI	WD14	1	BB93719_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Pper_Dia2013_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	CTTAGTGGCATTAATTCGDAATCC	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93719_Sspa_Oph2007_COI	WD14	1	BB93719_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Sspa_Oph2007_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	GAAGGCCTTCCGATGATC	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93719_Upin_Bot2011_COI	WD14	1	BB93719_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Upin_Bot2011_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	CTTTAATTACAGCGTTTTTATTGTTGT	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67349	WGS84	BB93719	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93719_Vgib_Wil2025_COI	WD14	1	BB93719_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Vgib_Wil2025_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	CCCCTCTTTACAAGTTCTTTAGGGT	141.86939
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93720_Cmae_Bot2011_COI	WD16	1	BB93720_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Cmae_Bot2011_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	ATGAACAGTCTATCCTCCTTTAG	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93720_Hsan_Wil2021_COI	WD16	1	BB93720_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Hsan_Wil2021_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	CCTGGGCCGGTATAGTAGGT	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93720_Mros_Gib2022_COI	WD16	1	BB93720_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Mros_Gib2022_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93720_Msal_Bot2012_COI	WD16	1	BB93720_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Msal_Bot2012_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	GYTAGTTCCRATGATGTTAGCTG	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93720_Pvir_Dia2013_COI	WD16	1	BB93720_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Pvir_Dia2013_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	CTTAGTGGCATTAATTCGDAATCC	141.86884
BB93720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67469	WGS84	BB93720	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93720_Rhar_Sim2018_COI	WD16	1	BB93720_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93720	Sample	BB93720	targeted	Absent	Sample	Rhar_Sim2018_COI	WD16	COI	45	BB93720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD16	CTGCTGCTATTGCTCATG	141.86884
BB93722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67312	WGS84	BB93722	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93722_Aamu_Bax2006_COI	WD19	1	BB93722_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93722	Sample	BB93722	targeted	Absent	Sample	Aamu_Bax2006_COI	WD19	COI	45	BB93722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD19	GCACAACCGGGATCTTTACTTC	141.87125
BB93722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67312	WGS84	BB93722	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93722_Pcan_Bot2011_IGS	WD19	1	BB93722_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93722	Sample	BB93722	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD19	IGS	45	BB93722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD19	CGTAATCCTCAGTACTGGCTA	141.87125
BB93722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67312	WGS84	BB93722	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93722_Sspa_Oph2007_COI	WD19	1	BB93722_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93722	Sample	BB93722	targeted	Absent	Sample	Sspa_Oph2007_COI	WD19	COI	45	BB93722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD19	GAAGGCCTTCCGATGATC	141.87125
BB93722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67312	WGS84	BB93722	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93722_Upin_Bot2011_COI	WD19	1	BB93722_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93722	Sample	BB93722	targeted	Absent	Sample	Upin_Bot2011_COI	WD19	COI	45	BB93722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD19	CTTTAATTACAGCGTTTTTATTGTTGT	141.87125
BB93723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66582	WGS84	BB93723	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93723_Mgig_Bot2012_COI	WD5	1	BB93723_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93723	Sample	BB93723	targeted	Absent	Sample	Mgig_Bot2012_COI	WD5	COI	45	BB93723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD5	TCTTATTCGTTGGAGACTTTATAACCCT	141.85
BB93724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67261	WGS84	BB93724	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93724_Cmae_Bot2011_COI	WD10	1	BB93724_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93724	Sample	BB93724	targeted	Absent	Sample	Cmae_Bot2011_COI	WD10	COI	45	BB93724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD10	ATGAACAGTCTATCCTCCTTTAG	141.86627
BB93724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67261	WGS84	BB93724	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93724_Msal_Bot2012_COI	WD10	1	BB93724_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93724	Sample	BB93724	targeted	Absent	Sample	Msal_Bot2012_COI	WD10	COI	45	BB93724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD10	GYTAGTTCCRATGATGTTAGCTG	141.86627
BB93726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81884	WGS84	BB93726	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93726_Aamu_Bax2006_COI	MC70	1	BB93726_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93726	Sample	BB93726	targeted	Absent	Sample	Aamu_Bax2006_COI	MC70	COI	45	BB93726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC70	GCACAACCGGGATCTTTACTTC	144.92457
BB93726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81884	WGS84	BB93726	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93726_Pcan_Bot2011_IGS	MC70	1	BB93726_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93726	Sample	BB93726	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC70	IGS	45	BB93726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC70	CGTAATCCTCAGTACTGGCTA	144.92457
BB93726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81884	WGS84	BB93726	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.61	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93726_Sspa_Oph2007_COI	MC70	1	BB93726_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93726	Sample	BB93726	targeted	Present	Sample	Sspa_Oph2007_COI	MC70	COI	45	BB93726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC70	GAAGGCCTTCCGATGATC	144.92457
BB93726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81884	WGS84	BB93726	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93726_Upin_Bot2011_COI	MC70	1	BB93726_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93726	Sample	BB93726	targeted	Absent	Sample	Upin_Bot2011_COI	MC70	COI	45	BB93726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC70	CTTTAATTACAGCGTTTTTATTGTTGT	144.92457
BB93727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82836	WGS84	BB93727	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.09	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93727_Mgig_Bot2012_COI	MC50	1	BB93727_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93727	Sample	BB93727	targeted	Present	Sample	Mgig_Bot2012_COI	MC50	COI	45	BB93727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC50	TCTTATTCGTTGGAGACTTTATAACCCT	144.89947
BB93728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86071	WGS84	BB93728	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93728_Cmae_Bot2011_COI	MC45	1	BB93728_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93728	Sample	BB93728	targeted	Absent	Sample	Cmae_Bot2011_COI	MC45	COI	45	BB93728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC45	ATGAACAGTCTATCCTCCTTTAG	144.9137
BB93728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86071	WGS84	BB93728	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93728_Msal_Bot2012_COI	MC45	1	BB93728_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93728	Sample	BB93728	targeted	Absent	Sample	Msal_Bot2012_COI	MC45	COI	45	BB93728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC45	GYTAGTTCCRATGATGTTAGCTG	144.9137
BB93730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84891	WGS84	BB93730	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.311	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93730_Mgig_Bot2012_COI	MC20	1	BB93730_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93730	Sample	BB93730	targeted	Present	Sample	Mgig_Bot2012_COI	MC20	COI	45	BB93730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC20	TCTTATTCGTTGGAGACTTTATAACCCT	144.91502
BB93731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8489	WGS84	BB93731	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93731_Cmae_Bot2011_COI	MC15	1	BB93731_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93731	Sample	BB93731	targeted	Absent	Sample	Cmae_Bot2011_COI	MC15	COI	45	BB93731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC15	ATGAACAGTCTATCCTCCTTTAG	144.91028
BB93731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8489	WGS84	BB93731	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93731_Msal_Bot2012_COI	MC15	1	BB93731_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93731	Sample	BB93731	targeted	Absent	Sample	Msal_Bot2012_COI	MC15	COI	45	BB93731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC15	GYTAGTTCCRATGATGTTAGCTG	144.91028
BB93733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84169	WGS84	BB93733	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93733_Aamu_Bax2006_COI	MC5	1	BB93733_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93733	Sample	BB93733	targeted	Absent	Sample	Aamu_Bax2006_COI	MC5	COI	45	BB93733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC5	GCACAACCGGGATCTTTACTTC	144.92949
BB93733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84169	WGS84	BB93733	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93733_Pcan_Bot2011_IGS	MC5	1	BB93733_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93733	Sample	BB93733	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC5	IGS	45	BB93733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC5	CGTAATCCTCAGTACTGGCTA	144.92949
BB93733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84169	WGS84	BB93733	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.208	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93733_Sspa_Oph2007_COI	MC5	1	BB93733_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93733	Sample	BB93733	targeted	Present	Sample	Sspa_Oph2007_COI	MC5	COI	45	BB93733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC5	GAAGGCCTTCCGATGATC	144.92949
BB93733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84169	WGS84	BB93733	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93733_Upin_Bot2011_COI	MC5	1	BB93733_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93733	Sample	BB93733	targeted	Absent	Sample	Upin_Bot2011_COI	MC5	COI	45	BB93733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC5	CTTTAATTACAGCGTTTTTATTGTTGT	144.92949
BB93734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82131	WGS84	BB93734	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.452	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93734_Mgig_Bot2012_COI	MC115	1	BB93734_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93734	Sample	BB93734	targeted	Present	Sample	Mgig_Bot2012_COI	MC115	COI	45	BB93734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC115	TCTTATTCGTTGGAGACTTTATAACCCT	144.90843
BB93735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93735	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93735_Cmae_Bot2011_COI	MC110	1	BB93735_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93735	Sample	BB93735	targeted	Absent	Sample	Cmae_Bot2011_COI	MC110	COI	45	BB93735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC110	ATGAACAGTCTATCCTCCTTTAG	144.91218
BB93735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93735	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93735_Msal_Bot2012_COI	MC110	1	BB93735_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93735	Sample	BB93735	targeted	Absent	Sample	Msal_Bot2012_COI	MC110	COI	45	BB93735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC110	GYTAGTTCCRATGATGTTAGCTG	144.91218
BB93737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81959	WGS84	BB93737	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93737_Aamu_Bax2006_COI	MC77	1	BB93737_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93737	Sample	BB93737	targeted	Absent	Sample	Aamu_Bax2006_COI	MC77	COI	45	BB93737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC77	GCACAACCGGGATCTTTACTTC	144.92662
BB93737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81959	WGS84	BB93737	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93737_Pcan_Bot2011_IGS	MC77	1	BB93737_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93737	Sample	BB93737	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC77	IGS	45	BB93737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC77	CGTAATCCTCAGTACTGGCTA	144.92662
BB93737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81959	WGS84	BB93737	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.655	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93737_Sspa_Oph2007_COI	MC77	1	BB93737_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93737	Sample	BB93737	targeted	Present	Sample	Sspa_Oph2007_COI	MC77	COI	45	BB93737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC77	GAAGGCCTTCCGATGATC	144.92662
BB93737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81959	WGS84	BB93737	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93737_Upin_Bot2011_COI	MC77	1	BB93737_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93737	Sample	BB93737	targeted	Absent	Sample	Upin_Bot2011_COI	MC77	COI	45	BB93737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC77	CTTTAATTACAGCGTTTTTATTGTTGT	144.92662
BB93738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81989	WGS84	BB93738	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.947	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93738_Mgig_Bot2012_COI	MC73	1	BB93738_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93738	Sample	BB93738	targeted	Present	Sample	Mgig_Bot2012_COI	MC73	COI	45	BB93738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC73	TCTTATTCGTTGGAGACTTTATAACCCT	144.92932
BB93739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82006	WGS84	BB93739	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93739_Cmae_Bot2011_COI	MC72	1	BB93739_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93739	Sample	BB93739	targeted	Absent	Sample	Cmae_Bot2011_COI	MC72	COI	45	BB93739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC72	ATGAACAGTCTATCCTCCTTTAG	144.93236
BB93739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82006	WGS84	BB93739	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93739_Msal_Bot2012_COI	MC72	1	BB93739_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93739	Sample	BB93739	targeted	Absent	Sample	Msal_Bot2012_COI	MC72	COI	45	BB93739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC72	GYTAGTTCCRATGATGTTAGCTG	144.93236
BB93740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81812	WGS84	BB93740	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93740_Aamu_Bax2006_COI	MC68	1	BB93740_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93740	Sample	BB93740	targeted	Absent	Sample	Aamu_Bax2006_COI	MC68	COI	45	BB93740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC68	GCACAACCGGGATCTTTACTTC	144.9219
BB93740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81812	WGS84	BB93740	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93740_Pcan_Bot2011_IGS	MC68	1	BB93740_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93740	Sample	BB93740	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC68	IGS	45	BB93740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC68	CGTAATCCTCAGTACTGGCTA	144.9219
BB93740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81812	WGS84	BB93740	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.04	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93740_Sspa_Oph2007_COI	MC68	1	BB93740_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93740	Sample	BB93740	targeted	Present	Sample	Sspa_Oph2007_COI	MC68	COI	45	BB93740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC68	GAAGGCCTTCCGATGATC	144.9219
BB93740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81812	WGS84	BB93740	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93740_Upin_Bot2011_COI	MC68	1	BB93740_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93740	Sample	BB93740	targeted	Absent	Sample	Upin_Bot2011_COI	MC68	COI	45	BB93740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC68	CTTTAATTACAGCGTTTTTATTGTTGT	144.9219
BB93741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81929	WGS84	BB93741	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.795	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93741_Mgig_Bot2012_COI	MC66	1	BB93741_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93741	Sample	BB93741	targeted	Present	Sample	Mgig_Bot2012_COI	MC66	COI	45	BB93741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC66	TCTTATTCGTTGGAGACTTTATAACCCT	144.91866
BB93742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81982	WGS84	BB93742	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93742_Cmae_Bot2011_COI	MC64	1	BB93742_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93742	Sample	BB93742	targeted	Absent	Sample	Cmae_Bot2011_COI	MC64	COI	45	BB93742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC64	ATGAACAGTCTATCCTCCTTTAG	144.91739
BB93742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81982	WGS84	BB93742	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93742_Msal_Bot2012_COI	MC64	1	BB93742_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93742	Sample	BB93742	targeted	Absent	Sample	Msal_Bot2012_COI	MC64	COI	45	BB93742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC64	GYTAGTTCCRATGATGTTAGCTG	144.91739
BB93744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82503	WGS84	BB93744	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.335	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93744_Aamu_Bax2006_COI	MC54	1	BB93744_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93744	Sample	BB93744	targeted	Present	Sample	Aamu_Bax2006_COI	MC54	COI	45	BB93744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC54	GCACAACCGGGATCTTTACTTC	144.9036
BB93744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82503	WGS84	BB93744	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93744_Pcan_Bot2011_IGS	MC54	1	BB93744_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93744	Sample	BB93744	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC54	IGS	45	BB93744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC54	CGTAATCCTCAGTACTGGCTA	144.9036
BB93744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82503	WGS84	BB93744	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.031	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93744_Sspa_Oph2007_COI	MC54	1	BB93744_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93744	Sample	BB93744	targeted	Present	Sample	Sspa_Oph2007_COI	MC54	COI	45	BB93744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC54	GAAGGCCTTCCGATGATC	144.9036
BB93744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82503	WGS84	BB93744	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93744_Upin_Bot2011_COI	MC54	1	BB93744_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93744	Sample	BB93744	targeted	Absent	Sample	Upin_Bot2011_COI	MC54	COI	45	BB93744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC54	CTTTAATTACAGCGTTTTTATTGTTGT	144.9036
BB93745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82512	WGS84	BB93745	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.06	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93745_Mgig_Bot2012_COI	MC53	1	BB93745_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93745	Sample	BB93745	targeted	Present	Sample	Mgig_Bot2012_COI	MC53	COI	45	BB93745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC53	TCTTATTCGTTGGAGACTTTATAACCCT	144.90113
BB93746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82613	WGS84	BB93746	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93746_Cmae_Bot2011_COI	MC52	1	BB93746_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93746	Sample	BB93746	targeted	Absent	Sample	Cmae_Bot2011_COI	MC52	COI	45	BB93746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC52	ATGAACAGTCTATCCTCCTTTAG	144.9012
BB93746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82613	WGS84	BB93746	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93746_Msal_Bot2012_COI	MC52	1	BB93746_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93746	Sample	BB93746	targeted	Absent	Sample	Msal_Bot2012_COI	MC52	COI	45	BB93746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC52	GYTAGTTCCRATGATGTTAGCTG	144.9012
BB93748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85994	WGS84	BB93748	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93748_Aamu_Bax2006_COI	MC47	1	BB93748_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93748	Sample	BB93748	targeted	Absent	Sample	Aamu_Bax2006_COI	MC47	COI	45	BB93748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC47	GCACAACCGGGATCTTTACTTC	144.91173
BB93748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85994	WGS84	BB93748	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93748_Pcan_Bot2011_IGS	MC47	1	BB93748_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93748	Sample	BB93748	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC47	IGS	45	BB93748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC47	CGTAATCCTCAGTACTGGCTA	144.91173
BB93748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85994	WGS84	BB93748	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.742	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93748_Sspa_Oph2007_COI	MC47	1	BB93748_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93748	Sample	BB93748	targeted	Present	Sample	Sspa_Oph2007_COI	MC47	COI	45	BB93748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC47	GAAGGCCTTCCGATGATC	144.91173
BB93748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85994	WGS84	BB93748	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93748_Upin_Bot2011_COI	MC47	1	BB93748_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93748	Sample	BB93748	targeted	Absent	Sample	Upin_Bot2011_COI	MC47	COI	45	BB93748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC47	CTTTAATTACAGCGTTTTTATTGTTGT	144.91173
BB93749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86107	WGS84	BB93749	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.447	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93749_Mgig_Bot2012_COI	MC46	1	BB93749_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93749	Sample	BB93749	targeted	Present	Sample	Mgig_Bot2012_COI	MC46	COI	45	BB93749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC46	TCTTATTCGTTGGAGACTTTATAACCCT	144.91072
BB93751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93751	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.023	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93751_Aamu_Bax2006_COI	MC43	1	BB93751_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93751	Sample	BB93751	targeted	Present	Sample	Aamu_Bax2006_COI	MC43	COI	45	BB93751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC43	GCACAACCGGGATCTTTACTTC	144.91536
BB93751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93751	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93751_Pcan_Bot2011_IGS	MC43	1	BB93751_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93751	Sample	BB93751	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC43	IGS	45	BB93751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC43	CGTAATCCTCAGTACTGGCTA	144.91536
BB93751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93751	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.118	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93751_Sspa_Oph2007_COI	MC43	1	BB93751_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93751	Sample	BB93751	targeted	Present	Sample	Sspa_Oph2007_COI	MC43	COI	45	BB93751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC43	GAAGGCCTTCCGATGATC	144.91536
BB93751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93751	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.634	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93751_Upin_Bot2011_COI	MC43	1	BB93751_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93751	Sample	BB93751	targeted	Present	Sample	Upin_Bot2011_COI	MC43	COI	45	BB93751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC43	CTTTAATTACAGCGTTTTTATTGTTGT	144.91536
BB93752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86221	WGS84	BB93752	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.849	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93752_Mgig_Bot2012_COI	MC42	1	BB93752_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93752	Sample	BB93752	targeted	Present	Sample	Mgig_Bot2012_COI	MC42	COI	45	BB93752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC42	TCTTATTCGTTGGAGACTTTATAACCCT	144.91719
BB93753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93753	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93753_Cmae_Bot2011_COI	MC41	1	BB93753_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93753	Sample	BB93753	targeted	Absent	Sample	Cmae_Bot2011_COI	MC41	COI	45	BB93753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC41	ATGAACAGTCTATCCTCCTTTAG	144.91334
BB93753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93753	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93753_Msal_Bot2012_COI	MC41	1	BB93753_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93753	Sample	BB93753	targeted	Absent	Sample	Msal_Bot2012_COI	MC41	COI	45	BB93753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC41	GYTAGTTCCRATGATGTTAGCTG	144.91334
BB93755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86339	WGS84	BB93755	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.447	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93755_Aamu_Bax2006_COI	MC38	1	BB93755_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93755	Sample	BB93755	targeted	Present	Sample	Aamu_Bax2006_COI	MC38	COI	45	BB93755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC38	GCACAACCGGGATCTTTACTTC	144.91381
BB93755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86339	WGS84	BB93755	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93755_Pcan_Bot2011_IGS	MC38	1	BB93755_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93755	Sample	BB93755	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC38	IGS	45	BB93755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC38	CGTAATCCTCAGTACTGGCTA	144.91381
BB93755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86339	WGS84	BB93755	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.579	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93755_Sspa_Oph2007_COI	MC38	1	BB93755_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93755	Sample	BB93755	targeted	Present	Sample	Sspa_Oph2007_COI	MC38	COI	45	BB93755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC38	GAAGGCCTTCCGATGATC	144.91381
BB93755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86339	WGS84	BB93755	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93755_Upin_Bot2011_COI	MC38	1	BB93755_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93755	Sample	BB93755	targeted	Absent	Sample	Upin_Bot2011_COI	MC38	COI	45	BB93755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC38	CTTTAATTACAGCGTTTTTATTGTTGT	144.91381
BB93756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86366	WGS84	BB93756	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.761	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93756_Mgig_Bot2012_COI	MC37	1	BB93756_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93756	Sample	BB93756	targeted	Present	Sample	Mgig_Bot2012_COI	MC37	COI	45	BB93756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC37	TCTTATTCGTTGGAGACTTTATAACCCT	144.91806
BB93757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86471	WGS84	BB93757	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93757_Cmae_Bot2011_COI	MC36	1	BB93757_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93757	Sample	BB93757	targeted	Absent	Sample	Cmae_Bot2011_COI	MC36	COI	45	BB93757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC36	ATGAACAGTCTATCCTCCTTTAG	144.91845
BB93757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86471	WGS84	BB93757	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93757_Msal_Bot2012_COI	MC36	1	BB93757_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93757	Sample	BB93757	targeted	Absent	Sample	Msal_Bot2012_COI	MC36	COI	45	BB93757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC36	GYTAGTTCCRATGATGTTAGCTG	144.91845
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93759_Aamu_Bax2006_COI	MC34	1	BB93759_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Absent	Sample	Aamu_Bax2006_COI	MC34	COI	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	GCACAACCGGGATCTTTACTTC	144.91507
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93759_Pcan_Bot2011_IGS	MC34	1	BB93759_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC34	IGS	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	CGTAATCCTCAGTACTGGCTA	144.91507
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.517	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93759_Sspa_Oph2007_COI	MC34	1	BB93759_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Present	Sample	Sspa_Oph2007_COI	MC34	COI	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	GAAGGCCTTCCGATGATC	144.91507
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93759_Upin_Bot2011_COI	MC34	1	BB93759_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Absent	Sample	Upin_Bot2011_COI	MC34	COI	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	CTTTAATTACAGCGTTTTTATTGTTGT	144.91507
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93760_Cmae_Bot2011_COI	MC32	1	BB93760_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Absent	Sample	Cmae_Bot2011_COI	MC32	COI	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	ATGAACAGTCTATCCTCCTTTAG	144.91742
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93760_Hsan_Wil2021_COI	MC32	1	BB93760_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Absent	Sample	Hsan_Wil2021_COI	MC32	COI	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	CCTGGGCCGGTATAGTAGGT	144.91742
BB93760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86562	WGS84	BB93760	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93760_Msal_Bot2012_COI	MC32	1	BB93760_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93760	Sample	BB93760	targeted	Absent	Sample	Msal_Bot2012_COI	MC32	COI	45	BB93760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC32	GYTAGTTCCRATGATGTTAGCTG	144.91742
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93762_Aamu_Bax2006_COI	MC26	1	BB93762_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Absent	Sample	Aamu_Bax2006_COI	MC26	COI	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	GCACAACCGGGATCTTTACTTC	144.90518
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93762_Pcan_Bot2011_IGS	MC26	1	BB93762_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC26	IGS	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	CGTAATCCTCAGTACTGGCTA	144.90518
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.24	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93762_Sspa_Oph2007_COI	MC26	1	BB93762_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Present	Sample	Sspa_Oph2007_COI	MC26	COI	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	GAAGGCCTTCCGATGATC	144.90518
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.219	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93762_Upin_Bot2011_COI	MC26	1	BB93762_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Present	Sample	Upin_Bot2011_COI	MC26	COI	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	CTTTAATTACAGCGTTTTTATTGTTGT	144.90518
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.523	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93763_Mgig_Bot2012_COI	MC24	1	BB93763_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Present	Sample	Mgig_Bot2012_COI	MC24	COI	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	TCTTATTCGTTGGAGACTTTATAACCCT	144.90579
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93764_Cmae_Bot2011_COI	MC22	1	BB93764_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Absent	Sample	Cmae_Bot2011_COI	MC22	COI	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	ATGAACAGTCTATCCTCCTTTAG	144.90784
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93764_Hsan_Wil2021_COI	MC22	1	BB93764_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Absent	Sample	Hsan_Wil2021_COI	MC22	COI	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	CCTGGGCCGGTATAGTAGGT	144.90784
BB93764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84557	WGS84	BB93764	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93764_Msal_Bot2012_COI	MC22	1	BB93764_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93764	Sample	BB93764	targeted	Absent	Sample	Msal_Bot2012_COI	MC22	COI	45	BB93764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC22	GYTAGTTCCRATGATGTTAGCTG	144.90784
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93766_Aamu_Bax2006_COI	MC16	1	BB93766_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Absent	Sample	Aamu_Bax2006_COI	MC16	COI	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	GCACAACCGGGATCTTTACTTC	144.91374
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93766_Pcan_Bot2011_IGS	MC16	1	BB93766_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC16	IGS	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	CGTAATCCTCAGTACTGGCTA	144.91374
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.733	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93766_Sspa_Oph2007_COI	MC16	1	BB93766_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Present	Sample	Sspa_Oph2007_COI	MC16	COI	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	GAAGGCCTTCCGATGATC	144.91374
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93766_Upin_Bot2011_COI	MC16	1	BB93766_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Absent	Sample	Upin_Bot2011_COI	MC16	COI	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	CTTTAATTACAGCGTTTTTATTGTTGT	144.91374
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.124	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93767_Mgig_Bot2012_COI	MC13	1	BB93767_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Present	Sample	Mgig_Bot2012_COI	MC13	COI	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	TCTTATTCGTTGGAGACTTTATAACCCT	144.91209
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93768_Cmae_Bot2011_COI	MC12	1	BB93768_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Absent	Sample	Cmae_Bot2011_COI	MC12	COI	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	ATGAACAGTCTATCCTCCTTTAG	144.91052
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.1442165	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93768_Hsan_Wil2021_COI	MC12	1	BB93768_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Present	Sample	Hsan_Wil2021_COI	MC12	COI	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	CCTGGGCCGGTATAGTAGGT	144.91052
BB93768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85003	WGS84	BB93768	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93768_Msal_Bot2012_COI	MC12	1	BB93768_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93768	Sample	BB93768	targeted	Absent	Sample	Msal_Bot2012_COI	MC12	COI	45	BB93768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC12	GYTAGTTCCRATGATGTTAGCTG	144.91052
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.973	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93770_Mgig_Bot2012_COI	MC98	1	BB93770_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Present	Sample	Mgig_Bot2012_COI	MC98	COI	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	TCTTATTCGTTGGAGACTTTATAACCCT	144.91328
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93771_Cmae_Bot2011_COI	MC96	1	BB93771_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Absent	Sample	Cmae_Bot2011_COI	MC96	COI	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	ATGAACAGTCTATCCTCCTTTAG	144.91359
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93771_Hsan_Wil2021_COI	MC96	1	BB93771_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Absent	Sample	Hsan_Wil2021_COI	MC96	COI	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	CCTGGGCCGGTATAGTAGGT	144.91359
BB93771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81439	WGS84	BB93771	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93771_Msal_Bot2012_COI	MC96	1	BB93771_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93771	Sample	BB93771	targeted	Absent	Sample	Msal_Bot2012_COI	MC96	COI	45	BB93771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC96	GYTAGTTCCRATGATGTTAGCTG	144.91359
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93773_Aamu_Bax2006_COI	MC91	1	BB93773_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Absent	Sample	Aamu_Bax2006_COI	MC91	COI	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	GCACAACCGGGATCTTTACTTC	144.91333
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93773_Pcan_Bot2011_IGS	MC91	1	BB93773_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC91	IGS	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	CGTAATCCTCAGTACTGGCTA	144.91333
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.123	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93773_Sspa_Oph2007_COI	MC91	1	BB93773_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Present	Sample	Sspa_Oph2007_COI	MC91	COI	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	GAAGGCCTTCCGATGATC	144.91333
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93773_Upin_Bot2011_COI	MC91	1	BB93773_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Absent	Sample	Upin_Bot2011_COI	MC91	COI	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	CTTTAATTACAGCGTTTTTATTGTTGT	144.91333
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.512	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93774_Mgig_Bot2012_COI	MC89	1	BB93774_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Present	Sample	Mgig_Bot2012_COI	MC89	COI	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	TCTTATTCGTTGGAGACTTTATAACCCT	144.91377
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93775_Cmae_Bot2011_COI	MC88	1	BB93775_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Absent	Sample	Cmae_Bot2011_COI	MC88	COI	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	ATGAACAGTCTATCCTCCTTTAG	144.91409
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93775_Hsan_Wil2021_COI	MC88	1	BB93775_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Absent	Sample	Hsan_Wil2021_COI	MC88	COI	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	CCTGGGCCGGTATAGTAGGT	144.91409
BB93775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8133	WGS84	BB93775	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93775_Msal_Bot2012_COI	MC88	1	BB93775_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93775	Sample	BB93775	targeted	Absent	Sample	Msal_Bot2012_COI	MC88	COI	45	BB93775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC88	GYTAGTTCCRATGATGTTAGCTG	144.91409
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93777_Aamu_Bax2006_COI	MC82	1	BB93777_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Absent	Sample	Aamu_Bax2006_COI	MC82	COI	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	GCACAACCGGGATCTTTACTTC	144.92242
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93777_Pcan_Bot2011_IGS	MC82	1	BB93777_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC82	IGS	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	CGTAATCCTCAGTACTGGCTA	144.92242
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.342	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93777_Sspa_Oph2007_COI	MC82	1	BB93777_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Present	Sample	Sspa_Oph2007_COI	MC82	COI	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	GAAGGCCTTCCGATGATC	144.92242
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93777_Upin_Bot2011_COI	MC82	1	BB93777_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Absent	Sample	Upin_Bot2011_COI	MC82	COI	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	CTTTAATTACAGCGTTTTTATTGTTGT	144.92242
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.124	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93778_Mgig_Bot2012_COI	MC81	1	BB93778_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Present	Sample	Mgig_Bot2012_COI	MC81	COI	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	TCTTATTCGTTGGAGACTTTATAACCCT	144.92449
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93779_Cmae_Bot2011_COI	MC7	1	BB93779_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Absent	Sample	Cmae_Bot2011_COI	MC7	COI	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	ATGAACAGTCTATCCTCCTTTAG	144.92731
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93779_Hsan_Wil2021_COI	MC7	1	BB93779_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Absent	Sample	Hsan_Wil2021_COI	MC7	COI	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	CCTGGGCCGGTATAGTAGGT	144.92731
BB93779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84283	WGS84	BB93779	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93779_Msal_Bot2012_COI	MC7	1	BB93779_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93779	Sample	BB93779	targeted	Absent	Sample	Msal_Bot2012_COI	MC7	COI	45	BB93779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC7	GYTAGTTCCRATGATGTTAGCTG	144.92731
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93780_Aamu_Bax2006_COI	MC4	1	BB93780_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Absent	Sample	Aamu_Bax2006_COI	MC4	COI	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	GCACAACCGGGATCTTTACTTC	144.93289
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93780_Pcan_Bot2011_IGS	MC4	1	BB93780_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC4	IGS	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	CGTAATCCTCAGTACTGGCTA	144.93289
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.3	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93780_Sspa_Oph2007_COI	MC4	1	BB93780_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Present	Sample	Sspa_Oph2007_COI	MC4	COI	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	GAAGGCCTTCCGATGATC	144.93289
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.369	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93780_Upin_Bot2011_COI	MC4	1	BB93780_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Present	Sample	Upin_Bot2011_COI	MC4	COI	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	CTTTAATTACAGCGTTTTTATTGTTGT	144.93289
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.462	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93781_Mgig_Bot2012_COI	MC2	1	BB93781_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Present	Sample	Mgig_Bot2012_COI	MC2	COI	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	TCTTATTCGTTGGAGACTTTATAACCCT	144.93023
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93782_Cmae_Bot2011_COI	MC1	1	BB93782_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Absent	Sample	Cmae_Bot2011_COI	MC1	COI	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	ATGAACAGTCTATCCTCCTTTAG	144.9291
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93782_Hsan_Wil2021_COI	MC1	1	BB93782_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Absent	Sample	Hsan_Wil2021_COI	MC1	COI	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	CCTGGGCCGGTATAGTAGGT	144.9291
BB93782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84425	WGS84	BB93782	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	22/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93782_Msal_Bot2012_COI	MC1	1	BB93782_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93782	Sample	BB93782	targeted	Absent	Sample	Msal_Bot2012_COI	MC1	COI	45	BB93782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC1	GYTAGTTCCRATGATGTTAGCTG	144.9291
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93784_Aamu_Bax2006_COI	MC108	1	BB93784_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Absent	Sample	Aamu_Bax2006_COI	MC108	COI	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	GCACAACCGGGATCTTTACTTC	144.91152
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93784_Pcan_Bot2011_IGS	MC108	1	BB93784_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC108	IGS	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	CGTAATCCTCAGTACTGGCTA	144.91152
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.235	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93784_Sspa_Oph2007_COI	MC108	1	BB93784_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Present	Sample	Sspa_Oph2007_COI	MC108	COI	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	GAAGGCCTTCCGATGATC	144.91152
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93784_Upin_Bot2011_COI	MC108	1	BB93784_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Absent	Sample	Upin_Bot2011_COI	MC108	COI	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	CTTTAATTACAGCGTTTTTATTGTTGT	144.91152
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.535	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93785_Mgig_Bot2012_COI	MC107	1	BB93785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Present	Sample	Mgig_Bot2012_COI	MC107	COI	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	TCTTATTCGTTGGAGACTTTATAACCCT	144.90595
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93786_Cmae_Bot2011_COI	MC106	1	BB93786_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Absent	Sample	Cmae_Bot2011_COI	MC106	COI	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	ATGAACAGTCTATCCTCCTTTAG	144.91331
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93786_Hsan_Wil2021_COI	MC106	1	BB93786_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Absent	Sample	Hsan_Wil2021_COI	MC106	COI	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	CCTGGGCCGGTATAGTAGGT	144.91331
BB93786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8181	WGS84	BB93786	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93786_Msal_Bot2012_COI	MC106	1	BB93786_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93786	Sample	BB93786	targeted	Absent	Sample	Msal_Bot2012_COI	MC106	COI	45	BB93786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC106	GYTAGTTCCRATGATGTTAGCTG	144.91331
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93788_Aamu_Bax2006_COI	MC102	1	BB93788_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Absent	Sample	Aamu_Bax2006_COI	MC102	COI	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	GCACAACCGGGATCTTTACTTC	144.91416
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93788_Pcan_Bot2011_IGS	MC102	1	BB93788_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC102	IGS	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	CGTAATCCTCAGTACTGGCTA	144.91416
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.805	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93788_Sspa_Oph2007_COI	MC102	1	BB93788_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Present	Sample	Sspa_Oph2007_COI	MC102	COI	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	GAAGGCCTTCCGATGATC	144.91416
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93788_Upin_Bot2011_COI	MC102	1	BB93788_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Absent	Sample	Upin_Bot2011_COI	MC102	COI	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	CTTTAATTACAGCGTTTTTATTGTTGT	144.91416
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.815	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93789_Mgig_Bot2012_COI	MC101	1	BB93789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Present	Sample	Mgig_Bot2012_COI	MC101	COI	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	TCTTATTCGTTGGAGACTTTATAACCCT	144.91999
BB93791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85956	WGS84	BB93791	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.53	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93791_Aamu_Bax2006_COI	MC126	1	BB93791_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93791	Sample	BB93791	targeted	Present	Sample	Aamu_Bax2006_COI	MC126	COI	45	BB93791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC126	GCACAACCGGGATCTTTACTTC	144.91138
BB93791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85956	WGS84	BB93791	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93791_Pcan_Bot2011_IGS	MC126	1	BB93791_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93791	Sample	BB93791	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC126	IGS	45	BB93791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC126	CGTAATCCTCAGTACTGGCTA	144.91138
BB93791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85956	WGS84	BB93791	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.656	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93791_Sspa_Oph2007_COI	MC126	1	BB93791_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93791	Sample	BB93791	targeted	Present	Sample	Sspa_Oph2007_COI	MC126	COI	45	BB93791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC126	GAAGGCCTTCCGATGATC	144.91138
BB93791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85956	WGS84	BB93791	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.684	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93791_Upin_Bot2011_COI	MC126	1	BB93791_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93791	Sample	BB93791	targeted	Present	Sample	Upin_Bot2011_COI	MC126	COI	45	BB93791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC126	CTTTAATTACAGCGTTTTTATTGTTGT	144.91138
BB93792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85883	WGS84	BB93792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.873	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93792_Mgig_Bot2012_COI	MC124	1	BB93792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93792	Sample	BB93792	targeted	Present	Sample	Mgig_Bot2012_COI	MC124	COI	45	BB93792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC124	TCTTATTCGTTGGAGACTTTATAACCCT	144.90837
BB93793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8597	WGS84	BB93793	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.714	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93793_Cmae_Bot2011_COI	MC123	1	BB93793_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93793	Sample	BB93793	targeted	Present	Sample	Cmae_Bot2011_COI	MC123	COI	45	BB93793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC123	ATGAACAGTCTATCCTCCTTTAG	144.90663
BB93793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8597	WGS84	BB93793	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93793_Msal_Bot2012_COI	MC123	1	BB93793_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93793	Sample	BB93793	targeted	Absent	Sample	Msal_Bot2012_COI	MC123	COI	45	BB93793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC123	GYTAGTTCCRATGATGTTAGCTG	144.90663
BB93795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93795	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93795_Aamu_Bax2006_COI	MC118	1	BB93795_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93795	Sample	BB93795	targeted	Absent	Sample	Aamu_Bax2006_COI	MC118	COI	45	BB93795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC118	GCACAACCGGGATCTTTACTTC	144.90629
BB93795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93795	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93795_Pcan_Bot2011_IGS	MC118	1	BB93795_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93795	Sample	BB93795	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC118	IGS	45	BB93795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC118	CGTAATCCTCAGTACTGGCTA	144.90629
BB93795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93795	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.651	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93795_Sspa_Oph2007_COI	MC118	1	BB93795_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93795	Sample	BB93795	targeted	Present	Sample	Sspa_Oph2007_COI	MC118	COI	45	BB93795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC118	GAAGGCCTTCCGATGATC	144.90629
BB93795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93795	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93795_Upin_Bot2011_COI	MC118	1	BB93795_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93795	Sample	BB93795	targeted	Absent	Sample	Upin_Bot2011_COI	MC118	COI	45	BB93795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC118	CTTTAATTACAGCGTTTTTATTGTTGT	144.90629
BB93796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82232	WGS84	BB93796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.232	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93796_Mgig_Bot2012_COI	MC117	1	BB93796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93796	Sample	BB93796	targeted	Present	Sample	Mgig_Bot2012_COI	MC117	COI	45	BB93796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC117	TCTTATTCGTTGGAGACTTTATAACCCT	144.90786
BB93803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4271	WGS84	BB93803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93803_Aamu_Bax2006_COI	BC40	1	BB93803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93803	Sample	BB93803	targeted	Absent	Sample	Aamu_Bax2006_COI	BC40	COI	45	BB93803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC40	GCACAACCGGGATCTTTACTTC	153.1238
BB93803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4271	WGS84	BB93803	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93803_Pcan_Bot2011_IGS	BC40	1	BB93803_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93803	Sample	BB93803	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC40	IGS	45	BB93803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC40	CGTAATCCTCAGTACTGGCTA	153.1238
BB93803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4271	WGS84	BB93803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93803_Sspa_Oph2007_COI	BC40	1	BB93803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93803	Sample	BB93803	targeted	Absent	Sample	Sspa_Oph2007_COI	BC40	COI	45	BB93803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC40	GAAGGCCTTCCGATGATC	153.1238
BB93803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4271	WGS84	BB93803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93803_Upin_Bot2011_COI	BC40	1	BB93803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93803	Sample	BB93803	targeted	Absent	Sample	Upin_Bot2011_COI	BC40	COI	45	BB93803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC40	CTTTAATTACAGCGTTTTTATTGTTGT	153.1238
BB93804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42155	WGS84	BB93804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93804_Mgig_Bot2012_COI	BC35	1	BB93804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93804	Sample	BB93804	targeted	Absent	Sample	Mgig_Bot2012_COI	BC35	COI	45	BB93804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC35	TCTTATTCGTTGGAGACTTTATAACCCT	153.13604
BB93805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44585	WGS84	BB93805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93805_Cmae_Bot2011_COI	BC65	1	BB93805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93805	Sample	BB93805	targeted	Absent	Sample	Cmae_Bot2011_COI	BC65	COI	45	BB93805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC65	ATGAACAGTCTATCCTCCTTTAG	153.08404
BB93805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44585	WGS84	BB93805	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93805_Msal_Bot2012_COI	BC65	1	BB93805_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93805	Sample	BB93805	targeted	Absent	Sample	Msal_Bot2012_COI	BC65	COI	45	BB93805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC65	GYTAGTTCCRATGATGTTAGCTG	153.08404
BB93807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3627	WGS84	BB93807	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93807_Aamu_Bax2006_COI	BC5	1	BB93807_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93807	Sample	BB93807	targeted	Absent	Sample	Aamu_Bax2006_COI	BC5	COI	45	BB93807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC5	GCACAACCGGGATCTTTACTTC	153.17652
BB93807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3627	WGS84	BB93807	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93807_Pcan_Bot2011_IGS	BC5	1	BB93807_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93807	Sample	BB93807	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC5	IGS	45	BB93807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC5	CGTAATCCTCAGTACTGGCTA	153.17652
BB93807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3627	WGS84	BB93807	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93807_Sspa_Oph2007_COI	BC5	1	BB93807_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93807	Sample	BB93807	targeted	Absent	Sample	Sspa_Oph2007_COI	BC5	COI	45	BB93807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC5	GAAGGCCTTCCGATGATC	153.17652
BB93807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3627	WGS84	BB93807	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93807_Upin_Bot2011_COI	BC5	1	BB93807_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93807	Sample	BB93807	targeted	Absent	Sample	Upin_Bot2011_COI	BC5	COI	45	BB93807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC5	CTTTAATTACAGCGTTTTTATTGTTGT	153.17652
BB93808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38487	WGS84	BB93808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93808_Mgig_Bot2012_COI	BC20	1	BB93808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93808	Sample	BB93808	targeted	Absent	Sample	Mgig_Bot2012_COI	BC20	COI	45	BB93808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC20	TCTTATTCGTTGGAGACTTTATAACCCT	153.15971
BB93809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37857	WGS84	BB93809	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93809_Cmae_Bot2011_COI	BC15	1	BB93809_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93809	Sample	BB93809	targeted	Absent	Sample	Cmae_Bot2011_COI	BC15	COI	45	BB93809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC15	ATGAACAGTCTATCCTCCTTTAG	153.1651
BB93809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37857	WGS84	BB93809	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93809_Msal_Bot2012_COI	BC15	1	BB93809_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93809	Sample	BB93809	targeted	Absent	Sample	Msal_Bot2012_COI	BC15	COI	45	BB93809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC15	GYTAGTTCCRATGATGTTAGCTG	153.1651
BB93810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44751	WGS84	BB93810	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93810_Aamu_Bax2006_COI	BC50	1	BB93810_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93810	Sample	BB93810	targeted	Absent	Sample	Aamu_Bax2006_COI	BC50	COI	45	BB93810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC50	GCACAACCGGGATCTTTACTTC	153.09566
BB93810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44751	WGS84	BB93810	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93810_Pcan_Bot2011_IGS	BC50	1	BB93810_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93810	Sample	BB93810	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC50	IGS	45	BB93810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC50	CGTAATCCTCAGTACTGGCTA	153.09566
BB93810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44751	WGS84	BB93810	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93810_Sspa_Oph2007_COI	BC50	1	BB93810_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93810	Sample	BB93810	targeted	Absent	Sample	Sspa_Oph2007_COI	BC50	COI	45	BB93810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC50	GAAGGCCTTCCGATGATC	153.09566
BB93810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44751	WGS84	BB93810	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93810_Upin_Bot2011_COI	BC50	1	BB93810_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93810	Sample	BB93810	targeted	Absent	Sample	Upin_Bot2011_COI	BC50	COI	45	BB93810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC50	CTTTAATTACAGCGTTTTTATTGTTGT	153.09566
BB93811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43258	WGS84	BB93811	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93811_Mgig_Bot2012_COI	BC48	1	BB93811_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93811	Sample	BB93811	targeted	Absent	Sample	Mgig_Bot2012_COI	BC48	COI	45	BB93811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC48	TCTTATTCGTTGGAGACTTTATAACCCT	153.11706
BB93812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43069	WGS84	BB93812	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93812_Cmae_Bot2011_COI	BC47	1	BB93812_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93812	Sample	BB93812	targeted	Absent	Sample	Cmae_Bot2011_COI	BC47	COI	45	BB93812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC47	ATGAACAGTCTATCCTCCTTTAG	153.11845
BB93812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43069	WGS84	BB93812	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93812_Msal_Bot2012_COI	BC47	1	BB93812_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93812	Sample	BB93812	targeted	Absent	Sample	Msal_Bot2012_COI	BC47	COI	45	BB93812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC47	GYTAGTTCCRATGATGTTAGCTG	153.11845
BB93814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42466	WGS84	BB93814	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93814_Aamu_Bax2006_COI	BC38	1	BB93814_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93814	Sample	BB93814	targeted	Absent	Sample	Aamu_Bax2006_COI	BC38	COI	45	BB93814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC38	GCACAACCGGGATCTTTACTTC	153.12943
BB93814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42466	WGS84	BB93814	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93814_Pcan_Bot2011_IGS	BC38	1	BB93814_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93814	Sample	BB93814	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC38	IGS	45	BB93814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC38	CGTAATCCTCAGTACTGGCTA	153.12943
BB93814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42466	WGS84	BB93814	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93814_Sspa_Oph2007_COI	BC38	1	BB93814_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93814	Sample	BB93814	targeted	Absent	Sample	Sspa_Oph2007_COI	BC38	COI	45	BB93814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC38	GAAGGCCTTCCGATGATC	153.12943
BB93814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42466	WGS84	BB93814	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93814_Upin_Bot2011_COI	BC38	1	BB93814_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93814	Sample	BB93814	targeted	Absent	Sample	Upin_Bot2011_COI	BC38	COI	45	BB93814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC38	CTTTAATTACAGCGTTTTTATTGTTGT	153.12943
BB93815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.41408	WGS84	BB93815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93815_Mgig_Bot2012_COI	BC34	1	BB93815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93815	Sample	BB93815	targeted	Absent	Sample	Mgig_Bot2012_COI	BC34	COI	45	BB93815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC34	TCTTATTCGTTGGAGACTTTATAACCCT	153.14362
BB93816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40652	WGS84	BB93816	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93816_Cmae_Bot2011_COI	BC33	1	BB93816_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93816	Sample	BB93816	targeted	Absent	Sample	Cmae_Bot2011_COI	BC33	COI	45	BB93816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC33	ATGAACAGTCTATCCTCCTTTAG	153.15089
BB93816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40652	WGS84	BB93816	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93816_Msal_Bot2012_COI	BC33	1	BB93816_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93816	Sample	BB93816	targeted	Absent	Sample	Msal_Bot2012_COI	BC33	COI	45	BB93816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC33	GYTAGTTCCRATGATGTTAGCTG	153.15089
BB93818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40084	WGS84	BB93818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93818_Aamu_Bax2006_COI	BC29	1	BB93818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93818	Sample	BB93818	targeted	Absent	Sample	Aamu_Bax2006_COI	BC29	COI	45	BB93818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC29	GCACAACCGGGATCTTTACTTC	153.15682
BB93818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40084	WGS84	BB93818	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93818_Pcan_Bot2011_IGS	BC29	1	BB93818_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93818	Sample	BB93818	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC29	IGS	45	BB93818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC29	CGTAATCCTCAGTACTGGCTA	153.15682
BB93818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40084	WGS84	BB93818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93818_Sspa_Oph2007_COI	BC29	1	BB93818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93818	Sample	BB93818	targeted	Absent	Sample	Sspa_Oph2007_COI	BC29	COI	45	BB93818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC29	GAAGGCCTTCCGATGATC	153.15682
BB93818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40084	WGS84	BB93818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93818_Upin_Bot2011_COI	BC29	1	BB93818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93818	Sample	BB93818	targeted	Absent	Sample	Upin_Bot2011_COI	BC29	COI	45	BB93818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC29	CTTTAATTACAGCGTTTTTATTGTTGT	153.15682
BB93819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40095	WGS84	BB93819	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93819_Mgig_Bot2012_COI	BC28	1	BB93819_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93819	Sample	BB93819	targeted	Absent	Sample	Mgig_Bot2012_COI	BC28	COI	45	BB93819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC28	TCTTATTCGTTGGAGACTTTATAACCCT	153.1607
BB93821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44624	WGS84	BB93821	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93821_Aamu_Bax2006_COI	BC62	1	BB93821_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93821	Sample	BB93821	targeted	Absent	Sample	Aamu_Bax2006_COI	BC62	COI	45	BB93821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC62	GCACAACCGGGATCTTTACTTC	153.07816
BB93821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44624	WGS84	BB93821	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93821_Pcan_Bot2011_IGS	BC62	1	BB93821_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93821	Sample	BB93821	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC62	IGS	45	BB93821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC62	CGTAATCCTCAGTACTGGCTA	153.07816
BB93821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44624	WGS84	BB93821	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93821_Sspa_Oph2007_COI	BC62	1	BB93821_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93821	Sample	BB93821	targeted	Absent	Sample	Sspa_Oph2007_COI	BC62	COI	45	BB93821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC62	GAAGGCCTTCCGATGATC	153.07816
BB93821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44624	WGS84	BB93821	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93821_Upin_Bot2011_COI	BC62	1	BB93821_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93821	Sample	BB93821	targeted	Absent	Sample	Upin_Bot2011_COI	BC62	COI	45	BB93821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC62	CTTTAATTACAGCGTTTTTATTGTTGT	153.07816
BB93822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44499	WGS84	BB93822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93822_Mgig_Bot2012_COI	BC61	1	BB93822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93822	Sample	BB93822	targeted	Absent	Sample	Mgig_Bot2012_COI	BC61	COI	45	BB93822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC61	TCTTATTCGTTGGAGACTTTATAACCCT	153.08037
BB93823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44525	WGS84	BB93823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93823_Cmae_Bot2011_COI	BC59	1	BB93823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93823	Sample	BB93823	targeted	Absent	Sample	Cmae_Bot2011_COI	BC59	COI	45	BB93823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC59	ATGAACAGTCTATCCTCCTTTAG	153.07854
BB93823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44525	WGS84	BB93823	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93823_Msal_Bot2012_COI	BC59	1	BB93823_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93823	Sample	BB93823	targeted	Absent	Sample	Msal_Bot2012_COI	BC59	COI	45	BB93823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC59	GYTAGTTCCRATGATGTTAGCTG	153.07854
BB93825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44414	WGS84	BB93825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93825_Aamu_Bax2006_COI	BC54	1	BB93825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93825	Sample	BB93825	targeted	Absent	Sample	Aamu_Bax2006_COI	BC54	COI	45	BB93825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC54	GCACAACCGGGATCTTTACTTC	153.07558
BB93825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44414	WGS84	BB93825	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93825_Pcan_Bot2011_IGS	BC54	1	BB93825_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93825	Sample	BB93825	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC54	IGS	45	BB93825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC54	CGTAATCCTCAGTACTGGCTA	153.07558
BB93825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44414	WGS84	BB93825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93825_Sspa_Oph2007_COI	BC54	1	BB93825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93825	Sample	BB93825	targeted	Absent	Sample	Sspa_Oph2007_COI	BC54	COI	45	BB93825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC54	GAAGGCCTTCCGATGATC	153.07558
BB93825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44414	WGS84	BB93825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93825_Upin_Bot2011_COI	BC54	1	BB93825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93825	Sample	BB93825	targeted	Absent	Sample	Upin_Bot2011_COI	BC54	COI	45	BB93825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC54	CTTTAATTACAGCGTTTTTATTGTTGT	153.07558
BB93826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44257	WGS84	BB93826	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93826_Mgig_Bot2012_COI	BC52	1	BB93826_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93826	Sample	BB93826	targeted	Absent	Sample	Mgig_Bot2012_COI	BC52	COI	45	BB93826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC52	TCTTATTCGTTGGAGACTTTATAACCCT	153.07162
BB93827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36951	WGS84	BB93827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93827_Cmae_Bot2011_COI	BC9	1	BB93827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93827	Sample	BB93827	targeted	Absent	Sample	Cmae_Bot2011_COI	BC9	COI	45	BB93827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC9	ATGAACAGTCTATCCTCCTTTAG	153.17173
BB93827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36951	WGS84	BB93827	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93827_Msal_Bot2012_COI	BC9	1	BB93827_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93827	Sample	BB93827	targeted	Absent	Sample	Msal_Bot2012_COI	BC9	COI	45	BB93827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC9	GYTAGTTCCRATGATGTTAGCTG	153.17173
BB93829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36126	WGS84	BB93829	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93829_Aamu_Bax2006_COI	BC4	1	BB93829_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93829	Sample	BB93829	targeted	Absent	Sample	Aamu_Bax2006_COI	BC4	COI	45	BB93829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC4	GCACAACCGGGATCTTTACTTC	153.17768
BB93829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36126	WGS84	BB93829	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93829_Pcan_Bot2011_IGS	BC4	1	BB93829_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93829	Sample	BB93829	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC4	IGS	45	BB93829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC4	CGTAATCCTCAGTACTGGCTA	153.17768
BB93829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36126	WGS84	BB93829	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93829_Sspa_Oph2007_COI	BC4	1	BB93829_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93829	Sample	BB93829	targeted	Absent	Sample	Sspa_Oph2007_COI	BC4	COI	45	BB93829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC4	GAAGGCCTTCCGATGATC	153.17768
BB93829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36126	WGS84	BB93829	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93829_Upin_Bot2011_COI	BC4	1	BB93829_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93829	Sample	BB93829	targeted	Absent	Sample	Upin_Bot2011_COI	BC4	COI	45	BB93829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC4	CTTTAATTACAGCGTTTTTATTGTTGT	153.17768
BB93830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35982	WGS84	BB93830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93830_Cmae_Bot2011_COI	BC3	1	BB93830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93830	Sample	BB93830	targeted	Absent	Sample	Cmae_Bot2011_COI	BC3	COI	45	BB93830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC3	ATGAACAGTCTATCCTCCTTTAG	153.17885
BB93830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35982	WGS84	BB93830	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93830_Msal_Bot2012_COI	BC3	1	BB93830_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93830	Sample	BB93830	targeted	Absent	Sample	Msal_Bot2012_COI	BC3	COI	45	BB93830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC3	GYTAGTTCCRATGATGTTAGCTG	153.17885
BB93832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38726	WGS84	BB93832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93832_Aamu_Bax2006_COI	BC22	1	BB93832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93832	Sample	BB93832	targeted	Absent	Sample	Aamu_Bax2006_COI	BC22	COI	45	BB93832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC22	GCACAACCGGGATCTTTACTTC	153.15893
BB93832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38726	WGS84	BB93832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93832_Pcan_Bot2011_IGS	BC22	1	BB93832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93832	Sample	BB93832	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC22	IGS	45	BB93832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC22	CGTAATCCTCAGTACTGGCTA	153.15893
BB93832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38726	WGS84	BB93832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93832_Sspa_Oph2007_COI	BC22	1	BB93832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93832	Sample	BB93832	targeted	Absent	Sample	Sspa_Oph2007_COI	BC22	COI	45	BB93832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC22	GAAGGCCTTCCGATGATC	153.15893
BB93832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38726	WGS84	BB93832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93832_Upin_Bot2011_COI	BC22	1	BB93832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93832	Sample	BB93832	targeted	Absent	Sample	Upin_Bot2011_COI	BC22	COI	45	BB93832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC22	CTTTAATTACAGCGTTTTTATTGTTGT	153.15893
BB93833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38259	WGS84	BB93833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93833_Mgig_Bot2012_COI	BC18	1	BB93833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93833	Sample	BB93833	targeted	Absent	Sample	Mgig_Bot2012_COI	BC18	COI	45	BB93833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC18	TCTTATTCGTTGGAGACTTTATAACCCT	153.16112
BB93834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38037	WGS84	BB93834	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93834_Cmae_Bot2011_COI	BC17	1	BB93834_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93834	Sample	BB93834	targeted	Absent	Sample	Cmae_Bot2011_COI	BC17	COI	45	BB93834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC17	ATGAACAGTCTATCCTCCTTTAG	153.16371
BB93834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38037	WGS84	BB93834	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93834_Msal_Bot2012_COI	BC17	1	BB93834_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93834	Sample	BB93834	targeted	Absent	Sample	Msal_Bot2012_COI	BC17	COI	45	BB93834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC17	GYTAGTTCCRATGATGTTAGCTG	153.16371
BB93836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37255	WGS84	BB93836	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93836_Aamu_Bax2006_COI	BC11	1	BB93836_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93836	Sample	BB93836	targeted	Absent	Sample	Aamu_Bax2006_COI	BC11	COI	45	BB93836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC11	GCACAACCGGGATCTTTACTTC	153.16947
BB93836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37255	WGS84	BB93836	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93836_Pcan_Bot2011_IGS	BC11	1	BB93836_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93836	Sample	BB93836	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC11	IGS	45	BB93836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC11	CGTAATCCTCAGTACTGGCTA	153.16947
BB93836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37255	WGS84	BB93836	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93836_Sspa_Oph2007_COI	BC11	1	BB93836_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93836	Sample	BB93836	targeted	Absent	Sample	Sspa_Oph2007_COI	BC11	COI	45	BB93836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC11	GAAGGCCTTCCGATGATC	153.16947
BB93836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37255	WGS84	BB93836	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93836_Upin_Bot2011_COI	BC11	1	BB93836_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93836	Sample	BB93836	targeted	Absent	Sample	Upin_Bot2011_COI	BC11	COI	45	BB93836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC11	CTTTAATTACAGCGTTTTTATTGTTGT	153.16947
BB93837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35649	WGS84	BB93837	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93837_Mgig_Bot2012_COI	BC1	1	BB93837_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93837	Sample	BB93837	targeted	Absent	Sample	Mgig_Bot2012_COI	BC1	COI	45	BB93837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC1	TCTTATTCGTTGGAGACTTTATAACCCT	153.18075
BB93838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44809	WGS84	BB93838	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93838_Cmae_Bot2011_COI	BC51	1	BB93838_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93838	Sample	BB93838	targeted	Absent	Sample	Cmae_Bot2011_COI	BC51	COI	45	BB93838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC51	ATGAACAGTCTATCCTCCTTTAG	153.09371
BB93838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44809	WGS84	BB93838	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93838_Msal_Bot2012_COI	BC51	1	BB93838_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93838	Sample	BB93838	targeted	Absent	Sample	Msal_Bot2012_COI	BC51	COI	45	BB93838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC51	GYTAGTTCCRATGATGTTAGCTG	153.09371
BB93854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8765	WGS84	BB93854	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93854_Aamu_Bax2006_COI	HC20	1	BB93854_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93854	Sample	BB93854	targeted	Absent	Sample	Aamu_Bax2006_COI	HC20	COI	45	BB93854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC20	GCACAACCGGGATCTTTACTTC	147.3401
BB93854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8765	WGS84	BB93854	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93854_Pcan_Bot2011_IGS	HC20	1	BB93854_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93854	Sample	BB93854	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC20	IGS	45	BB93854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC20	CGTAATCCTCAGTACTGGCTA	147.3401
BB93854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8765	WGS84	BB93854	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93854_Sspa_Oph2007_COI	HC20	1	BB93854_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93854	Sample	BB93854	targeted	Absent	Sample	Sspa_Oph2007_COI	HC20	COI	45	BB93854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC20	GAAGGCCTTCCGATGATC	147.3401
BB93854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8765	WGS84	BB93854	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93854_Upin_Bot2011_COI	HC20	1	BB93854_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93854	Sample	BB93854	targeted	Absent	Sample	Upin_Bot2011_COI	HC20	COI	45	BB93854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC20	CTTTAATTACAGCGTTTTTATTGTTGT	147.3401
BB93855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.844	WGS84	BB93855	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.721	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93855_Mgig_Bot2012_COI	HC5	1	BB93855_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93855	Sample	BB93855	targeted	Present	Sample	Mgig_Bot2012_COI	HC5	COI	45	BB93855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC5	TCTTATTCGTTGGAGACTTTATAACCCT	147.3277
BB93856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8745	WGS84	BB93856	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93856_Cmae_Bot2011_COI	HC23	1	BB93856_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93856	Sample	BB93856	targeted	Absent	Sample	Cmae_Bot2011_COI	HC23	COI	45	BB93856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC23	ATGAACAGTCTATCCTCCTTTAG	147.3381
BB93856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8745	WGS84	BB93856	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93856_Msal_Bot2012_COI	HC23	1	BB93856_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93856	Sample	BB93856	targeted	Absent	Sample	Msal_Bot2012_COI	HC23	COI	45	BB93856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC23	GYTAGTTCCRATGATGTTAGCTG	147.3381
BB93858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8786	WGS84	BB93858	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93858_Aamu_Bax2006_COI	HC19	1	BB93858_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93858	Sample	BB93858	targeted	Absent	Sample	Aamu_Bax2006_COI	HC19	COI	45	BB93858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC19	GCACAACCGGGATCTTTACTTC	147.3421
BB93858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8786	WGS84	BB93858	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93858_Pcan_Bot2011_IGS	HC19	1	BB93858_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93858	Sample	BB93858	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC19	IGS	45	BB93858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC19	CGTAATCCTCAGTACTGGCTA	147.3421
BB93858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8786	WGS84	BB93858	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93858_Sspa_Oph2007_COI	HC19	1	BB93858_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93858	Sample	BB93858	targeted	Absent	Sample	Sspa_Oph2007_COI	HC19	COI	45	BB93858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC19	GAAGGCCTTCCGATGATC	147.3421
BB93858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8786	WGS84	BB93858	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93858_Upin_Bot2011_COI	HC19	1	BB93858_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93858	Sample	BB93858	targeted	Absent	Sample	Upin_Bot2011_COI	HC19	COI	45	BB93858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC19	CTTTAATTACAGCGTTTTTATTGTTGT	147.3421
BB93859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8818	WGS84	BB93859	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.562	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93859_Mgig_Bot2012_COI	HC17	1	BB93859_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93859	Sample	BB93859	targeted	Present	Sample	Mgig_Bot2012_COI	HC17	COI	45	BB93859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC17	TCTTATTCGTTGGAGACTTTATAACCCT	147.3426
BB93861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8859	WGS84	BB93861	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.965	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93861_Aamu_Bax2006_COI	HC12	1	BB93861_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93861	Sample	BB93861	targeted	Present	Sample	Aamu_Bax2006_COI	HC12	COI	45	BB93861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC12	GCACAACCGGGATCTTTACTTC	147.3385
BB93861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8859	WGS84	BB93861	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93861_Pcan_Bot2011_IGS	HC12	1	BB93861_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93861	Sample	BB93861	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC12	IGS	45	BB93861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC12	CGTAATCCTCAGTACTGGCTA	147.3385
BB93861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8859	WGS84	BB93861	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93861_Sspa_Oph2007_COI	HC12	1	BB93861_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93861	Sample	BB93861	targeted	Absent	Sample	Sspa_Oph2007_COI	HC12	COI	45	BB93861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC12	GAAGGCCTTCCGATGATC	147.3385
BB93861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8859	WGS84	BB93861	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93861_Upin_Bot2011_COI	HC12	1	BB93861_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93861	Sample	BB93861	targeted	Absent	Sample	Upin_Bot2011_COI	HC12	COI	45	BB93861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC12	CTTTAATTACAGCGTTTTTATTGTTGT	147.3385
BB93862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8828	WGS84	BB93862	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.689	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93862_Mgig_Bot2012_COI	HC13	1	BB93862_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93862	Sample	BB93862	targeted	Present	Sample	Mgig_Bot2012_COI	HC13	COI	45	BB93862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC13	TCTTATTCGTTGGAGACTTTATAACCCT	147.3363
BB93863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8485	WGS84	BB93863	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93863_Cmae_Bot2011_COI	HC11	1	BB93863_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93863	Sample	BB93863	targeted	Absent	Sample	Cmae_Bot2011_COI	HC11	COI	45	BB93863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC11	ATGAACAGTCTATCCTCCTTTAG	147.3283
BB93863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8485	WGS84	BB93863	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93863_Msal_Bot2012_COI	HC11	1	BB93863_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93863	Sample	BB93863	targeted	Absent	Sample	Msal_Bot2012_COI	HC11	COI	45	BB93863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC11	GYTAGTTCCRATGATGTTAGCTG	147.3283
BB93865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93865	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93865_Aamu_Bax2006_COI	HC8	1	BB93865_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93865	Sample	BB93865	targeted	Absent	Sample	Aamu_Bax2006_COI	HC8	COI	45	BB93865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC8	GCACAACCGGGATCTTTACTTC	147.3242
BB93865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93865	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93865_Pcan_Bot2011_IGS	HC8	1	BB93865_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93865	Sample	BB93865	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC8	IGS	45	BB93865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC8	CGTAATCCTCAGTACTGGCTA	147.3242
BB93865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93865	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93865_Sspa_Oph2007_COI	HC8	1	BB93865_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93865	Sample	BB93865	targeted	Absent	Sample	Sspa_Oph2007_COI	HC8	COI	45	BB93865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC8	GAAGGCCTTCCGATGATC	147.3242
BB93865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93865	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93865_Upin_Bot2011_COI	HC8	1	BB93865_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93865	Sample	BB93865	targeted	Absent	Sample	Upin_Bot2011_COI	HC8	COI	45	BB93865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC8	CTTTAATTACAGCGTTTTTATTGTTGT	147.3242
BB93866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93866	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.219	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93866_Mgig_Bot2012_COI	HC4	1	BB93866_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93866	Sample	BB93866	targeted	Present	Sample	Mgig_Bot2012_COI	HC4	COI	45	BB93866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC4	TCTTATTCGTTGGAGACTTTATAACCCT	147.3295
BB93867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8284	WGS84	BB93867	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93867_Cmae_Bot2011_COI	HC3	1	BB93867_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93867	Sample	BB93867	targeted	Absent	Sample	Cmae_Bot2011_COI	HC3	COI	45	BB93867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC3	ATGAACAGTCTATCCTCCTTTAG	147.3166
BB93867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8284	WGS84	BB93867	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93867_Msal_Bot2012_COI	HC3	1	BB93867_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93867	Sample	BB93867	targeted	Absent	Sample	Msal_Bot2012_COI	HC3	COI	45	BB93867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC3	GYTAGTTCCRATGATGTTAGCTG	147.3166
BB93874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8071	WGS84	BB93874	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93874_Cmae_Bot2011_COI	GC110	1	BB93874_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93874	Sample	BB93874	targeted	Absent	Sample	Cmae_Bot2011_COI	GC110	COI	45	BB93874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC110	ATGAACAGTCTATCCTCCTTTAG	151.21783
BB93874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8071	WGS84	BB93874	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93874_Msal_Bot2012_COI	GC110	1	BB93874_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93874	Sample	BB93874	targeted	Absent	Sample	Msal_Bot2012_COI	GC110	COI	45	BB93874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC110	GYTAGTTCCRATGATGTTAGCTG	151.21783
BB93876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85487	WGS84	BB93876	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93876_Aamu_Bax2006_COI	GC10	1	BB93876_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93876	Sample	BB93876	targeted	Absent	Sample	Aamu_Bax2006_COI	GC10	COI	45	BB93876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC10	GCACAACCGGGATCTTTACTTC	151.31994
BB93876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85487	WGS84	BB93876	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93876_Pcan_Bot2011_IGS	GC10	1	BB93876_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93876	Sample	BB93876	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC10	IGS	45	BB93876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC10	CGTAATCCTCAGTACTGGCTA	151.31994
BB93876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85487	WGS84	BB93876	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93876_Sspa_Oph2007_COI	GC10	1	BB93876_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93876	Sample	BB93876	targeted	Absent	Sample	Sspa_Oph2007_COI	GC10	COI	45	BB93876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC10	GAAGGCCTTCCGATGATC	151.31994
BB93876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85487	WGS84	BB93876	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93876_Upin_Bot2011_COI	GC10	1	BB93876_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93876	Sample	BB93876	targeted	Absent	Sample	Upin_Bot2011_COI	GC10	COI	45	BB93876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC10	CTTTAATTACAGCGTTTTTATTGTTGT	151.31994
BB93877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85248	WGS84	BB93877	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93877_Mgig_Bot2012_COI	GC5	1	BB93877_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93877	Sample	BB93877	targeted	Absent	Sample	Mgig_Bot2012_COI	GC5	COI	45	BB93877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC5	TCTTATTCGTTGGAGACTTTATAACCCT	151.31446
BB93878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79136	WGS84	BB93878	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93878_Cmae_Bot2011_COI	GC100	1	BB93878_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93878	Sample	BB93878	targeted	Absent	Sample	Cmae_Bot2011_COI	GC100	COI	45	BB93878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC100	ATGAACAGTCTATCCTCCTTTAG	151.17803
BB93878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79136	WGS84	BB93878	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93878_Msal_Bot2012_COI	GC100	1	BB93878_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93878	Sample	BB93878	targeted	Absent	Sample	Msal_Bot2012_COI	GC100	COI	45	BB93878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC100	GYTAGTTCCRATGATGTTAGCTG	151.17803
BB93880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78768	WGS84	BB93880	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93880_Mgig_Bot2012_COI	GC90	1	BB93880_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93880	Sample	BB93880	targeted	Absent	Sample	Mgig_Bot2012_COI	GC90	COI	45	BB93880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC90	TCTTATTCGTTGGAGACTTTATAACCCT	151.17541
BB93881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7864	WGS84	BB93881	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93881_Cmae_Bot2011_COI	GC85	1	BB93881_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93881	Sample	BB93881	targeted	Absent	Sample	Cmae_Bot2011_COI	GC85	COI	45	BB93881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC85	ATGAACAGTCTATCCTCCTTTAG	151.17264
BB93881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7864	WGS84	BB93881	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93881_Msal_Bot2012_COI	GC85	1	BB93881_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93881	Sample	BB93881	targeted	Absent	Sample	Msal_Bot2012_COI	GC85	COI	45	BB93881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC85	GYTAGTTCCRATGATGTTAGCTG	151.17264
BB93883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7791	WGS84	BB93883	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93883_Aamu_Bax2006_COI	GC75	1	BB93883_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93883	Sample	BB93883	targeted	Absent	Sample	Aamu_Bax2006_COI	GC75	COI	45	BB93883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC75	GCACAACCGGGATCTTTACTTC	151.19661
BB93883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7791	WGS84	BB93883	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93883_Pcan_Bot2011_IGS	GC75	1	BB93883_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93883	Sample	BB93883	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC75	IGS	45	BB93883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC75	CGTAATCCTCAGTACTGGCTA	151.19661
BB93883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7791	WGS84	BB93883	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93883_Sspa_Oph2007_COI	GC75	1	BB93883_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93883	Sample	BB93883	targeted	Absent	Sample	Sspa_Oph2007_COI	GC75	COI	45	BB93883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC75	GAAGGCCTTCCGATGATC	151.19661
BB93883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7791	WGS84	BB93883	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93883_Upin_Bot2011_COI	GC75	1	BB93883_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93883	Sample	BB93883	targeted	Absent	Sample	Upin_Bot2011_COI	GC75	COI	45	BB93883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC75	CTTTAATTACAGCGTTTTTATTGTTGT	151.19661
BB93884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81554	WGS84	BB93884	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93884_Mgig_Bot2012_COI	GC65	1	BB93884_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93884	Sample	BB93884	targeted	Absent	Sample	Mgig_Bot2012_COI	GC65	COI	45	BB93884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC65	TCTTATTCGTTGGAGACTTTATAACCCT	151.23926
BB93885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82641	WGS84	BB93885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93885_Cmae_Bot2011_COI	GC60	1	BB93885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93885	Sample	BB93885	targeted	Absent	Sample	Cmae_Bot2011_COI	GC60	COI	45	BB93885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC60	ATGAACAGTCTATCCTCCTTTAG	151.24732
BB93885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82641	WGS84	BB93885	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93885_Msal_Bot2012_COI	GC60	1	BB93885_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93885	Sample	BB93885	targeted	Absent	Sample	Msal_Bot2012_COI	GC60	COI	45	BB93885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC60	GYTAGTTCCRATGATGTTAGCTG	151.24732
BB93887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82125	WGS84	BB93887	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93887_Aamu_Bax2006_COI	GC50	1	BB93887_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93887	Sample	BB93887	targeted	Absent	Sample	Aamu_Bax2006_COI	GC50	COI	45	BB93887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC50	GCACAACCGGGATCTTTACTTC	151.23987
BB93887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82125	WGS84	BB93887	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93887_Pcan_Bot2011_IGS	GC50	1	BB93887_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93887	Sample	BB93887	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC50	IGS	45	BB93887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC50	CGTAATCCTCAGTACTGGCTA	151.23987
BB93887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82125	WGS84	BB93887	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93887_Sspa_Oph2007_COI	GC50	1	BB93887_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93887	Sample	BB93887	targeted	Absent	Sample	Sspa_Oph2007_COI	GC50	COI	45	BB93887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC50	GAAGGCCTTCCGATGATC	151.23987
BB93887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82125	WGS84	BB93887	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93887_Upin_Bot2011_COI	GC50	1	BB93887_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93887	Sample	BB93887	targeted	Absent	Sample	Upin_Bot2011_COI	GC50	COI	45	BB93887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC50	CTTTAATTACAGCGTTTTTATTGTTGT	151.23987
BB93888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82403	WGS84	BB93888	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93888_Mgig_Bot2012_COI	GC40	1	BB93888_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93888	Sample	BB93888	targeted	Absent	Sample	Mgig_Bot2012_COI	GC40	COI	45	BB93888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC40	TCTTATTCGTTGGAGACTTTATAACCCT	151.2451
BB93889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83417	WGS84	BB93889	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93889_Cmae_Bot2011_COI	GC35	1	BB93889_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93889	Sample	BB93889	targeted	Absent	Sample	Cmae_Bot2011_COI	GC35	COI	45	BB93889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC35	ATGAACAGTCTATCCTCCTTTAG	151.2657
BB93889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83417	WGS84	BB93889	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93889_Msal_Bot2012_COI	GC35	1	BB93889_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93889	Sample	BB93889	targeted	Absent	Sample	Msal_Bot2012_COI	GC35	COI	45	BB93889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC35	GYTAGTTCCRATGATGTTAGCTG	151.2657
BB93890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80771	WGS84	BB93890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93890_Aamu_Bax2006_COI	GC109	1	BB93890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93890	Sample	BB93890	targeted	Absent	Sample	Aamu_Bax2006_COI	GC109	COI	45	BB93890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC109	GCACAACCGGGATCTTTACTTC	151.21666
BB93890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80771	WGS84	BB93890	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93890_Pcan_Bot2011_IGS	GC109	1	BB93890_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93890	Sample	BB93890	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC109	IGS	45	BB93890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC109	CGTAATCCTCAGTACTGGCTA	151.21666
BB93890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80771	WGS84	BB93890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93890_Sspa_Oph2007_COI	GC109	1	BB93890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93890	Sample	BB93890	targeted	Absent	Sample	Sspa_Oph2007_COI	GC109	COI	45	BB93890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC109	GAAGGCCTTCCGATGATC	151.21666
BB93890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80771	WGS84	BB93890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93890_Upin_Bot2011_COI	GC109	1	BB93890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93890	Sample	BB93890	targeted	Absent	Sample	Upin_Bot2011_COI	GC109	COI	45	BB93890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC109	CTTTAATTACAGCGTTTTTATTGTTGT	151.21666
BB93891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8081	WGS84	BB93891	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93891_Mgig_Bot2012_COI	GC107	1	BB93891_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93891	Sample	BB93891	targeted	Absent	Sample	Mgig_Bot2012_COI	GC107	COI	45	BB93891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC107	TCTTATTCGTTGGAGACTTTATAACCCT	151.22028
BB93892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80503	WGS84	BB93892	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93892_Cmae_Bot2011_COI	GC103	1	BB93892_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93892	Sample	BB93892	targeted	Absent	Sample	Cmae_Bot2011_COI	GC103	COI	45	BB93892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC103	ATGAACAGTCTATCCTCCTTTAG	151.21647
BB93892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80503	WGS84	BB93892	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93892_Msal_Bot2012_COI	GC103	1	BB93892_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93892	Sample	BB93892	targeted	Absent	Sample	Msal_Bot2012_COI	GC103	COI	45	BB93892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC103	GYTAGTTCCRATGATGTTAGCTG	151.21647
BB93894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85458	WGS84	BB93894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93894_Aamu_Bax2006_COI	GC12	1	BB93894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93894	Sample	BB93894	targeted	Absent	Sample	Aamu_Bax2006_COI	GC12	COI	45	BB93894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC12	GCACAACCGGGATCTTTACTTC	151.32258
BB93894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85458	WGS84	BB93894	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93894_Pcan_Bot2011_IGS	GC12	1	BB93894_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93894	Sample	BB93894	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC12	IGS	45	BB93894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC12	CGTAATCCTCAGTACTGGCTA	151.32258
BB93894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85458	WGS84	BB93894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93894_Sspa_Oph2007_COI	GC12	1	BB93894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93894	Sample	BB93894	targeted	Absent	Sample	Sspa_Oph2007_COI	GC12	COI	45	BB93894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC12	GAAGGCCTTCCGATGATC	151.32258
BB93894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85458	WGS84	BB93894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93894_Upin_Bot2011_COI	GC12	1	BB93894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93894	Sample	BB93894	targeted	Absent	Sample	Upin_Bot2011_COI	GC12	COI	45	BB93894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC12	CTTTAATTACAGCGTTTTTATTGTTGT	151.32258
BB93895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85269	WGS84	BB93895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93895_Mgig_Bot2012_COI	GC8	1	BB93895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93895	Sample	BB93895	targeted	Absent	Sample	Mgig_Bot2012_COI	GC8	COI	45	BB93895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC8	TCTTATTCGTTGGAGACTTTATAACCCT	151.31994
BB93896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85242	WGS84	BB93896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93896_Cmae_Bot2011_COI	GC6	1	BB93896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93896	Sample	BB93896	targeted	Absent	Sample	Cmae_Bot2011_COI	GC6	COI	45	BB93896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC6	ATGAACAGTCTATCCTCCTTTAG	151.31635
BB93896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85242	WGS84	BB93896	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93896_Msal_Bot2012_COI	GC6	1	BB93896_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93896	Sample	BB93896	targeted	Absent	Sample	Msal_Bot2012_COI	GC6	COI	45	BB93896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC6	GYTAGTTCCRATGATGTTAGCTG	151.31635
BB93898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85254	WGS84	BB93898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93898_Aamu_Bax2006_COI	GC1	1	BB93898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93898	Sample	BB93898	targeted	Absent	Sample	Aamu_Bax2006_COI	GC1	COI	45	BB93898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC1	GCACAACCGGGATCTTTACTTC	151.30846
BB93898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85254	WGS84	BB93898	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93898_Pcan_Bot2011_IGS	GC1	1	BB93898_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93898	Sample	BB93898	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC1	IGS	45	BB93898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC1	CGTAATCCTCAGTACTGGCTA	151.30846
BB93898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85254	WGS84	BB93898	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93898_Sspa_Oph2007_COI	GC1	1	BB93898_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93898	Sample	BB93898	targeted	Absent	Sample	Sspa_Oph2007_COI	GC1	COI	45	BB93898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC1	GAAGGCCTTCCGATGATC	151.30846
BB93898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85254	WGS84	BB93898	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93898_Upin_Bot2011_COI	GC1	1	BB93898_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93898	Sample	BB93898	targeted	Absent	Sample	Upin_Bot2011_COI	GC1	COI	45	BB93898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC1	CTTTAATTACAGCGTTTTTATTGTTGT	151.30846
BB93899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79153	WGS84	BB93899	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93899_Mgig_Bot2012_COI	GC98	1	BB93899_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93899	Sample	BB93899	targeted	Absent	Sample	Mgig_Bot2012_COI	GC98	COI	45	BB93899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC98	TCTTATTCGTTGGAGACTTTATAACCCT	151.18068
BB93900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79182	WGS84	BB93900	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93900_Cmae_Bot2011_COI	GC96	1	BB93900_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93900	Sample	BB93900	targeted	Absent	Sample	Cmae_Bot2011_COI	GC96	COI	45	BB93900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC96	ATGAACAGTCTATCCTCCTTTAG	151.18352
BB93900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79182	WGS84	BB93900	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93900_Msal_Bot2012_COI	GC96	1	BB93900_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93900	Sample	BB93900	targeted	Absent	Sample	Msal_Bot2012_COI	GC96	COI	45	BB93900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC96	GYTAGTTCCRATGATGTTAGCTG	151.18352
BB93902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78759	WGS84	BB93902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93902_Aamu_Bax2006_COI	GC88	1	BB93902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93902	Sample	BB93902	targeted	Absent	Sample	Aamu_Bax2006_COI	GC88	COI	45	BB93902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC88	GCACAACCGGGATCTTTACTTC	151.17442
BB93902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78759	WGS84	BB93902	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93902_Pcan_Bot2011_IGS	GC88	1	BB93902_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93902	Sample	BB93902	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC88	IGS	45	BB93902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC88	CGTAATCCTCAGTACTGGCTA	151.17442
BB93902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78759	WGS84	BB93902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93902_Sspa_Oph2007_COI	GC88	1	BB93902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93902	Sample	BB93902	targeted	Absent	Sample	Sspa_Oph2007_COI	GC88	COI	45	BB93902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC88	GAAGGCCTTCCGATGATC	151.17442
BB93902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78759	WGS84	BB93902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93902_Upin_Bot2011_COI	GC88	1	BB93902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93902	Sample	BB93902	targeted	Absent	Sample	Upin_Bot2011_COI	GC88	COI	45	BB93902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC88	CTTTAATTACAGCGTTTTTATTGTTGT	151.17442
BB93903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78545	WGS84	BB93903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93903_Mgig_Bot2012_COI	GC87	1	BB93903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93903	Sample	BB93903	targeted	Absent	Sample	Mgig_Bot2012_COI	GC87	COI	45	BB93903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC87	TCTTATTCGTTGGAGACTTTATAACCCT	151.17523
BB93904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78472	WGS84	BB93904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93904_Cmae_Bot2011_COI	GC84	1	BB93904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93904	Sample	BB93904	targeted	Absent	Sample	Cmae_Bot2011_COI	GC84	COI	45	BB93904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC84	ATGAACAGTCTATCCTCCTTTAG	151.17289
BB93904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78472	WGS84	BB93904	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93904_Msal_Bot2012_COI	GC84	1	BB93904_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93904	Sample	BB93904	targeted	Absent	Sample	Msal_Bot2012_COI	GC84	COI	45	BB93904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC84	GYTAGTTCCRATGATGTTAGCTG	151.17289
BC05867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83717	WGS84	BC05867	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05867_Cmae_Bot2011_COI	GC14	1	BC05867_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05867	Sample	BC05867	targeted	Absent	Sample	Cmae_Bot2011_COI	GC14	COI	45	BC05867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC14	ATGAACAGTCTATCCTCCTTTAG	151.27458
BC05867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83717	WGS84	BC05867	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05867_Msal_Bot2012_COI	GC14	1	BC05867_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05867	Sample	BC05867	targeted	Absent	Sample	Msal_Bot2012_COI	GC14	COI	45	BC05867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC14	GYTAGTTCCRATGATGTTAGCTG	151.27458
BC05869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.835	WGS84	BC05869	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05869_Aamu_Bax2006_COI	GC18	1	BC05869_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05869	Sample	BC05869	targeted	Absent	Sample	Aamu_Bax2006_COI	GC18	COI	45	BC05869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC18	GCACAACCGGGATCTTTACTTC	151.27193
BC05869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.835	WGS84	BC05869	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05869_Pcan_Bot2011_IGS	GC18	1	BC05869_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05869	Sample	BC05869	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC18	IGS	45	BC05869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC18	CGTAATCCTCAGTACTGGCTA	151.27193
BC05869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.835	WGS84	BC05869	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05869_Sspa_Oph2007_COI	GC18	1	BC05869_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05869	Sample	BC05869	targeted	Absent	Sample	Sspa_Oph2007_COI	GC18	COI	45	BC05869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC18	GAAGGCCTTCCGATGATC	151.27193
BC05869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.835	WGS84	BC05869	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05869_Upin_Bot2011_COI	GC18	1	BC05869_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05869	Sample	BC05869	targeted	Absent	Sample	Upin_Bot2011_COI	GC18	COI	45	BC05869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC18	CTTTAATTACAGCGTTTTTATTGTTGT	151.27193
BC05870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83558	WGS84	BC05870	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05870_Cmae_Bot2011_COI	GC19	1	BC05870_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05870	Sample	BC05870	targeted	Absent	Sample	Cmae_Bot2011_COI	GC19	COI	45	BC05870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC19	ATGAACAGTCTATCCTCCTTTAG	151.26913
BC05870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83558	WGS84	BC05870	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05870_Msal_Bot2012_COI	GC19	1	BC05870_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05870	Sample	BC05870	targeted	Absent	Sample	Msal_Bot2012_COI	GC19	COI	45	BC05870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC19	GYTAGTTCCRATGATGTTAGCTG	151.26913
BC05872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83097	WGS84	BC05872	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05872_Aamu_Bax2006_COI	GC23	1	BC05872_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05872	Sample	BC05872	targeted	Absent	Sample	Aamu_Bax2006_COI	GC23	COI	45	BC05872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC23	GCACAACCGGGATCTTTACTTC	151.2546
BC05872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83097	WGS84	BC05872	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05872_Pcan_Bot2011_IGS	GC23	1	BC05872_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05872	Sample	BC05872	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC23	IGS	45	BC05872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC23	CGTAATCCTCAGTACTGGCTA	151.2546
BC05872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83097	WGS84	BC05872	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05872_Sspa_Oph2007_COI	GC23	1	BC05872_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05872	Sample	BC05872	targeted	Absent	Sample	Sspa_Oph2007_COI	GC23	COI	45	BC05872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC23	GAAGGCCTTCCGATGATC	151.2546
BC05872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83097	WGS84	BC05872	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05872_Upin_Bot2011_COI	GC23	1	BC05872_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05872	Sample	BC05872	targeted	Absent	Sample	Upin_Bot2011_COI	GC23	COI	45	BC05872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC23	CTTTAATTACAGCGTTTTTATTGTTGT	151.2546
BC05873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83485	WGS84	BC05873	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05873_Mgig_Bot2012_COI	GC34	1	BC05873_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05873	Sample	BC05873	targeted	Absent	Sample	Mgig_Bot2012_COI	GC34	COI	45	BC05873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC34	TCTTATTCGTTGGAGACTTTATAACCCT	151.26891
BC05874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83216	WGS84	BC05874	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05874_Cmae_Bot2011_COI	GC37	1	BC05874_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05874	Sample	BC05874	targeted	Absent	Sample	Cmae_Bot2011_COI	GC37	COI	45	BC05874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC37	ATGAACAGTCTATCCTCCTTTAG	151.26233
BC05874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83216	WGS84	BC05874	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05874_Msal_Bot2012_COI	GC37	1	BC05874_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05874	Sample	BC05874	targeted	Absent	Sample	Msal_Bot2012_COI	GC37	COI	45	BC05874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC37	GYTAGTTCCRATGATGTTAGCTG	151.26233
BC05876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79447	WGS84	BC05876	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05876_Aamu_Bax2006_COI	GC72	1	BC05876_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05876	Sample	BC05876	targeted	Absent	Sample	Aamu_Bax2006_COI	GC72	COI	45	BC05876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC72	GCACAACCGGGATCTTTACTTC	151.21339
BC05876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79447	WGS84	BC05876	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05876_Pcan_Bot2011_IGS	GC72	1	BC05876_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05876	Sample	BC05876	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC72	IGS	45	BC05876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC72	CGTAATCCTCAGTACTGGCTA	151.21339
BC05876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79447	WGS84	BC05876	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05876_Sspa_Oph2007_COI	GC72	1	BC05876_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05876	Sample	BC05876	targeted	Absent	Sample	Sspa_Oph2007_COI	GC72	COI	45	BC05876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC72	GAAGGCCTTCCGATGATC	151.21339
BC05876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79447	WGS84	BC05876	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05876_Upin_Bot2011_COI	GC72	1	BC05876_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05876	Sample	BC05876	targeted	Absent	Sample	Upin_Bot2011_COI	GC72	COI	45	BC05876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC72	CTTTAATTACAGCGTTTTTATTGTTGT	151.21339
BC05877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79074	WGS84	BC05877	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05877_Mgig_Bot2012_COI	GC73	1	BC05877_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05877	Sample	BC05877	targeted	Absent	Sample	Mgig_Bot2012_COI	GC73	COI	45	BC05877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC73	TCTTATTCGTTGGAGACTTTATAACCCT	151.20948
BC05878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81709	WGS84	BC05878	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05878_Cmae_Bot2011_COI	GC24	1	BC05878_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05878	Sample	BC05878	targeted	Absent	Sample	Cmae_Bot2011_COI	GC24	COI	45	BC05878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC24	ATGAACAGTCTATCCTCCTTTAG	151.23804
BC05878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81709	WGS84	BC05878	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05878_Msal_Bot2012_COI	GC24	1	BC05878_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05878	Sample	BC05878	targeted	Absent	Sample	Msal_Bot2012_COI	GC24	COI	45	BC05878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC24	GYTAGTTCCRATGATGTTAGCTG	151.23804
BC05880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81635	WGS84	BC05880	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05880_Mgig_Bot2012_COI	GC27	1	BC05880_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05880	Sample	BC05880	targeted	Absent	Sample	Mgig_Bot2012_COI	GC27	COI	45	BC05880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC27	TCTTATTCGTTGGAGACTTTATAACCCT	151.23642
BC05881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81904	WGS84	BC05881	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05881_Cmae_Bot2011_COI	GC29	1	BC05881_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05881	Sample	BC05881	targeted	Absent	Sample	Cmae_Bot2011_COI	GC29	COI	45	BC05881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC29	ATGAACAGTCTATCCTCCTTTAG	151.24007
BC05881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81904	WGS84	BC05881	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05881_Msal_Bot2012_COI	GC29	1	BC05881_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05881	Sample	BC05881	targeted	Absent	Sample	Msal_Bot2012_COI	GC29	COI	45	BC05881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC29	GYTAGTTCCRATGATGTTAGCTG	151.24007
BC05883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82391	WGS84	BC05883	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05883_Aamu_Bax2006_COI	GC32	1	BC05883_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05883	Sample	BC05883	targeted	Absent	Sample	Aamu_Bax2006_COI	GC32	COI	45	BC05883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC32	GCACAACCGGGATCTTTACTTC	151.24455
BC05883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82391	WGS84	BC05883	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05883_Pcan_Bot2011_IGS	GC32	1	BC05883_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05883	Sample	BC05883	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC32	IGS	45	BC05883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC32	CGTAATCCTCAGTACTGGCTA	151.24455
BC05883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82391	WGS84	BC05883	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05883_Sspa_Oph2007_COI	GC32	1	BC05883_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05883	Sample	BC05883	targeted	Absent	Sample	Sspa_Oph2007_COI	GC32	COI	45	BC05883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC32	GAAGGCCTTCCGATGATC	151.24455
BC05883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82391	WGS84	BC05883	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05883_Upin_Bot2011_COI	GC32	1	BC05883_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05883	Sample	BC05883	targeted	Absent	Sample	Upin_Bot2011_COI	GC32	COI	45	BC05883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC32	CTTTAATTACAGCGTTTTTATTGTTGT	151.24455
BC05884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82267	WGS84	BC05884	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05884_Mgig_Bot2012_COI	GC42	1	BC05884_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05884	Sample	BC05884	targeted	Absent	Sample	Mgig_Bot2012_COI	GC42	COI	45	BC05884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC42	TCTTATTCGTTGGAGACTTTATAACCCT	151.24423
BC05885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8232	WGS84	BC05885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05885_Cmae_Bot2011_COI	GC44	1	BC05885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05885	Sample	BC05885	targeted	Absent	Sample	Cmae_Bot2011_COI	GC44	COI	45	BC05885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC44	ATGAACAGTCTATCCTCCTTTAG	151.24118
BC05885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8232	WGS84	BC05885	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05885_Msal_Bot2012_COI	GC44	1	BC05885_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05885	Sample	BC05885	targeted	Absent	Sample	Msal_Bot2012_COI	GC44	COI	45	BC05885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC44	GYTAGTTCCRATGATGTTAGCTG	151.24118
BC05887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82011	WGS84	BC05887	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05887_Aamu_Bax2006_COI	GC51	1	BC05887_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05887	Sample	BC05887	targeted	Absent	Sample	Aamu_Bax2006_COI	GC51	COI	45	BC05887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC51	GCACAACCGGGATCTTTACTTC	151.23989
BC05887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82011	WGS84	BC05887	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05887_Pcan_Bot2011_IGS	GC51	1	BC05887_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05887	Sample	BC05887	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC51	IGS	45	BC05887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC51	CGTAATCCTCAGTACTGGCTA	151.23989
BC05887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82011	WGS84	BC05887	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05887_Sspa_Oph2007_COI	GC51	1	BC05887_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05887	Sample	BC05887	targeted	Absent	Sample	Sspa_Oph2007_COI	GC51	COI	45	BC05887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC51	GAAGGCCTTCCGATGATC	151.23989
BC05887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82011	WGS84	BC05887	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05887_Upin_Bot2011_COI	GC51	1	BC05887_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05887	Sample	BC05887	targeted	Absent	Sample	Upin_Bot2011_COI	GC51	COI	45	BC05887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC51	CTTTAATTACAGCGTTTTTATTGTTGT	151.23989
BC05888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81964	WGS84	BC05888	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05888_Mgig_Bot2012_COI	GC53	1	BC05888_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05888	Sample	BC05888	targeted	Absent	Sample	Mgig_Bot2012_COI	GC53	COI	45	BC05888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC53	TCTTATTCGTTGGAGACTTTATAACCCT	151.23886
BC05889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82546	WGS84	BC05889	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05889_Cmae_Bot2011_COI	GC56	1	BC05889_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05889	Sample	BC05889	targeted	Absent	Sample	Cmae_Bot2011_COI	GC56	COI	45	BC05889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC56	ATGAACAGTCTATCCTCCTTTAG	151.24655
BC05889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82546	WGS84	BC05889	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05889_Msal_Bot2012_COI	GC56	1	BC05889_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05889	Sample	BC05889	targeted	Absent	Sample	Msal_Bot2012_COI	GC56	COI	45	BC05889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC56	GYTAGTTCCRATGATGTTAGCTG	151.24655
BC05890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82703	WGS84	BC05890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05890_Aamu_Bax2006_COI	GC59	1	BC05890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05890	Sample	BC05890	targeted	Absent	Sample	Aamu_Bax2006_COI	GC59	COI	45	BC05890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC59	GCACAACCGGGATCTTTACTTC	151.24639
BC05890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82703	WGS84	BC05890	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05890_Pcan_Bot2011_IGS	GC59	1	BC05890_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05890	Sample	BC05890	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC59	IGS	45	BC05890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC59	CGTAATCCTCAGTACTGGCTA	151.24639
BC05890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82703	WGS84	BC05890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05890_Sspa_Oph2007_COI	GC59	1	BC05890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05890	Sample	BC05890	targeted	Absent	Sample	Sspa_Oph2007_COI	GC59	COI	45	BC05890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC59	GAAGGCCTTCCGATGATC	151.24639
BC05890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82703	WGS84	BC05890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05890_Upin_Bot2011_COI	GC59	1	BC05890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05890	Sample	BC05890	targeted	Absent	Sample	Upin_Bot2011_COI	GC59	COI	45	BC05890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC59	CTTTAATTACAGCGTTTTTATTGTTGT	151.24639
BC05891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82553	WGS84	BC05891	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05891_Mgig_Bot2012_COI	GC61	1	BC05891_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05891	Sample	BC05891	targeted	Absent	Sample	Mgig_Bot2012_COI	GC61	COI	45	BC05891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC61	TCTTATTCGTTGGAGACTTTATAACCCT	151.2492
BC05892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82287	WGS84	BC05892	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05892_Cmae_Bot2011_COI	GC62	1	BC05892_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05892	Sample	BC05892	targeted	Absent	Sample	Cmae_Bot2011_COI	GC62	COI	45	BC05892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC62	ATGAACAGTCTATCCTCCTTTAG	151.24617
BC05892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82287	WGS84	BC05892	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05892_Msal_Bot2012_COI	GC62	1	BC05892_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05892	Sample	BC05892	targeted	Absent	Sample	Msal_Bot2012_COI	GC62	COI	45	BC05892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC62	GYTAGTTCCRATGATGTTAGCTG	151.24617
BC05894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81761	WGS84	BC05894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05894_Aamu_Bax2006_COI	GC64	1	BC05894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05894	Sample	BC05894	targeted	Absent	Sample	Aamu_Bax2006_COI	GC64	COI	45	BC05894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC64	GCACAACCGGGATCTTTACTTC	151.24103
BC05894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81761	WGS84	BC05894	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05894_Pcan_Bot2011_IGS	GC64	1	BC05894_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05894	Sample	BC05894	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC64	IGS	45	BC05894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC64	CGTAATCCTCAGTACTGGCTA	151.24103
BC05894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81761	WGS84	BC05894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05894_Sspa_Oph2007_COI	GC64	1	BC05894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05894	Sample	BC05894	targeted	Absent	Sample	Sspa_Oph2007_COI	GC64	COI	45	BC05894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC64	GAAGGCCTTCCGATGATC	151.24103
BC05894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81761	WGS84	BC05894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05894_Upin_Bot2011_COI	GC64	1	BC05894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05894	Sample	BC05894	targeted	Absent	Sample	Upin_Bot2011_COI	GC64	COI	45	BC05894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC64	CTTTAATTACAGCGTTTTTATTGTTGT	151.24103
BC05895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81432	WGS84	BC05895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05895_Mgig_Bot2012_COI	GC66	1	BC05895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05895	Sample	BC05895	targeted	Absent	Sample	Mgig_Bot2012_COI	GC66	COI	45	BC05895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC66	TCTTATTCGTTGGAGACTTTATAACCCT	151.23819
BC05896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82193	WGS84	BC05896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05896_Cmae_Bot2011_COI	GC111	1	BC05896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05896	Sample	BC05896	targeted	Absent	Sample	Cmae_Bot2011_COI	GC111	COI	45	BC05896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC111	ATGAACAGTCTATCCTCCTTTAG	151.24544
BC05896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82193	WGS84	BC05896	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05896_Msal_Bot2012_COI	GC111	1	BC05896_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05896	Sample	BC05896	targeted	Absent	Sample	Msal_Bot2012_COI	GC111	COI	45	BC05896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC111	GYTAGTTCCRATGATGTTAGCTG	151.24544
BC05898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78291	WGS84	BC05898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05898_Aamu_Bax2006_COI	GC78	1	BC05898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05898	Sample	BC05898	targeted	Absent	Sample	Aamu_Bax2006_COI	GC78	COI	45	BC05898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC78	GCACAACCGGGATCTTTACTTC	151.19983
BC05898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78291	WGS84	BC05898	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05898_Pcan_Bot2011_IGS	GC78	1	BC05898_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05898	Sample	BC05898	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC78	IGS	45	BC05898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC78	CGTAATCCTCAGTACTGGCTA	151.19983
BC05898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78291	WGS84	BC05898	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05898_Sspa_Oph2007_COI	GC78	1	BC05898_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05898	Sample	BC05898	targeted	Absent	Sample	Sspa_Oph2007_COI	GC78	COI	45	BC05898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC78	GAAGGCCTTCCGATGATC	151.19983
BC05898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78291	WGS84	BC05898	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05898_Upin_Bot2011_COI	GC78	1	BC05898_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05898	Sample	BC05898	targeted	Absent	Sample	Upin_Bot2011_COI	GC78	COI	45	BC05898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC78	CTTTAATTACAGCGTTTTTATTGTTGT	151.19983
BC05899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.77805	WGS84	BC05899	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05899_Mgig_Bot2012_COI	GC81	1	BC05899_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05899	Sample	BC05899	targeted	Absent	Sample	Mgig_Bot2012_COI	GC81	COI	45	BC05899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC81	TCTTATTCGTTGGAGACTTTATAACCCT	151.19493
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.098	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06765_Cmae_Bot2011_COI	T5-01	1	BC06765_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Present	Sample	Cmae_Bot2011_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	ATGAACAGTCTATCCTCCTTTAG	147.81438
BC06765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06765	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06765_Mros_Gib2022_COI	T5-01	1	BC06765_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06765	Sample	BC06765	targeted	Absent	Sample	Mros_Gib2022_COI	T5-01	COI	45	BC06765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81438
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06767_Aamu_Bax2006_COI	T5-03	1	BC06767_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Absent	Sample	Aamu_Bax2006_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	GCACAACCGGGATCTTTACTTC	147.87443
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06767_Pcan_Bot2011_IGS	T5-03	1	BC06767_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-03	IGS	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	CGTAATCCTCAGTACTGGCTA	147.87443
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06767_Sspa_Oph2007_COI	T5-03	1	BC06767_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	GAAGGCCTTCCGATGATC	147.87443
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.7	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06767_Upin_Bot2011_COI	T5-03	1	BC06767_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Present	Sample	Upin_Bot2011_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.87443
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06768_Cjap_Sim2018_COI	T5-04	1	BC06768_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87403
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	17/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06768_Mgig_Bot2012_COI	T5-04	1	BC06768_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.87403
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06768_Mjap_Gib2022_COI	T5-04	1	BC06768_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	AATTTAATTGTGACTAGGCATGGTCTG	147.87403
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06769_Cmae_Bot2011_COI	T5-05	1	BC06769_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	ATGAACAGTCTATCCTCCTTTAG	147.87326
BC06769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85574	WGS84	BC06769	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06769_Mros_Gib2022_COI	T5-05	1	BC06769_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06769	Sample	BC06769	targeted	Absent	Sample	Mros_Gib2022_COI	T5-05	COI	45	BC06769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87326
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06770_Aamu_Bax2006_COI	T5-06	1	BC06770_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Aamu_Bax2006_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	GCACAACCGGGATCTTTACTTC	147.87262
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06770_Pcan_Bot2011_IGS	T5-06	1	BC06770_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-06	IGS	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	CGTAATCCTCAGTACTGGCTA	147.87262
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06770_Sspa_Oph2007_COI	T5-06	1	BC06770_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	GAAGGCCTTCCGATGATC	147.87262
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.324	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06770_Upin_Bot2011_COI	T5-06	1	BC06770_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Present	Sample	Upin_Bot2011_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.87262
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06771_Cjap_Sim2018_COI	T5-07	1	BC06771_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30203
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06771_Mgig_Bot2012_COI	T5-07	1	BC06771_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.30203
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06771_Mjap_Gib2022_COI	T5-07	1	BC06771_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	AATTTAATTGTGACTAGGCATGGTCTG	147.30203
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06772_Cmae_Bot2011_COI	T5-08	1	BC06772_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	ATGAACAGTCTATCCTCCTTTAG	147.3022
BC06772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82728	WGS84	BC06772	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06772_Mros_Gib2022_COI	T5-08	1	BC06772_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06772	Sample	BC06772	targeted	Absent	Sample	Mros_Gib2022_COI	T5-08	COI	45	BC06772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.3022
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.05	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06774_Aamu_Bax2006_COI	T5-10	1	BC06774_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Present	Sample	Aamu_Bax2006_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	GCACAACCGGGATCTTTACTTC	147.33697
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06774_Pcan_Bot2011_IGS	T5-10	1	BC06774_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-10	IGS	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	CGTAATCCTCAGTACTGGCTA	147.33697
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06774_Sspa_Oph2007_COI	T5-10	1	BC06774_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	GAAGGCCTTCCGATGATC	147.33697
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06774_Upin_Bot2011_COI	T5-10	1	BC06774_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Absent	Sample	Upin_Bot2011_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.33697
BC06775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87812	WGS84	BC06775	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06775_Cjap_Sim2018_COI	T5-11	1	BC06775_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06775	Sample	BC06775	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-11	COI	45	BC06775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34163
BC06775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87812	WGS84	BC06775	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06775_Mgig_Bot2012_COI	T5-11	1	BC06775_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06775	Sample	BC06775	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-11	COI	45	BC06775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.34163
BC06776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88112	WGS84	BC06776	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06776_Cmae_Bot2011_COI	T5-12	1	BC06776_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06776	Sample	BC06776	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-12	COI	45	BC06776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-12	ATGAACAGTCTATCCTCCTTTAG	147.34198
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.074	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06778_Aamu_Bax2006_COI	T5-14	1	BC06778_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Present	Sample	Aamu_Bax2006_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	GCACAACCGGGATCTTTACTTC	147.33702
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06778_Pcan_Bot2011_IGS	T5-14	1	BC06778_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-14	IGS	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	CGTAATCCTCAGTACTGGCTA	147.33702
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06778_Sspa_Oph2007_COI	T5-14	1	BC06778_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	GAAGGCCTTCCGATGATC	147.33702
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06778_Upin_Bot2011_COI	T5-14	1	BC06778_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Absent	Sample	Upin_Bot2011_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.33702
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06779_Cjap_Sim2018_COI	T5-15	1	BC06779_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53158
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.114	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	21/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06779_Mgig_Bot2012_COI	T5-15	1	BC06779_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Present	Sample	Mgig_Bot2012_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.53158
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06779_Mjap_Gib2022_COI	T5-15	1	BC06779_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	AATTTAATTGTGACTAGGCATGGTCTG	147.53158
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.526	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06781_Aamu_Bax2006_COI	T5-17	1	BC06781_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Present	Sample	Aamu_Bax2006_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	GCACAACCGGGATCTTTACTTC	147.33607
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06781_Pcan_Bot2011_IGS	T5-17	1	BC06781_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-17	IGS	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	CGTAATCCTCAGTACTGGCTA	147.33607
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06781_Sspa_Oph2007_COI	T5-17	1	BC06781_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	GAAGGCCTTCCGATGATC	147.33607
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06781_Upin_Bot2011_COI	T5-17	1	BC06781_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Absent	Sample	Upin_Bot2011_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	CTTTAATTACAGCGTTTTTATTGTTGT	147.33607
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06782_Cjap_Sim2018_COI	T5-18	1	BC06782_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53312
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	26/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06782_Mgig_Bot2012_COI	T5-18	1	BC06782_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	TCTTATTCGTTGGAGACTTTATAACCCT	147.53312
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06782_Mjap_Gib2022_COI	T5-18	1	BC06782_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	AATTTAATTGTGACTAGGCATGGTCTG	147.53312
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.979	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06783_Cmae_Bot2011_COI	T5-19	1	BC06783_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Present	Sample	Cmae_Bot2011_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	ATGAACAGTCTATCCTCCTTTAG	147.51957
BC06783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96205	WGS84	BC06783	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.872	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06783_Mros_Gib2022_COI	T5-19	1	BC06783_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06783	Sample	BC06783	targeted	Present	Sample	Mros_Gib2022_COI	T5-19	COI	45	BC06783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-19	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.51957
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.495	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06785_Aamu_Bax2006_COI	T6-02	1	BC06785_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Present	Sample	Aamu_Bax2006_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	GCACAACCGGGATCTTTACTTC	147.303
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06785_Pcan_Bot2011_IGS	T6-02	1	BC06785_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-02	IGS	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	CGTAATCCTCAGTACTGGCTA	147.303
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06785_Sspa_Oph2007_COI	T6-02	1	BC06785_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	GAAGGCCTTCCGATGATC	147.303
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06785_Upin_Bot2011_COI	T6-02	1	BC06785_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Absent	Sample	Upin_Bot2011_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.303
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06786_Cjap_Sim2018_COI	T6-03	1	BC06786_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30193
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	23.94	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06786_Mgig_Bot2012_COI	T6-03	1	BC06786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Present	Sample	Mgig_Bot2012_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.30193
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06786_Mjap_Gib2022_COI	T6-03	1	BC06786_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	AATTTAATTGTGACTAGGCATGGTCTG	147.30193
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06787_Cmae_Bot2011_COI	T6-04	1	BC06787_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	ATGAACAGTCTATCCTCCTTTAG	147.33639
BC06787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87312	WGS84	BC06787	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06787_Mros_Gib2022_COI	T6-04	1	BC06787_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06787	Sample	BC06787	targeted	Absent	Sample	Mros_Gib2022_COI	T6-04	COI	45	BC06787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33639
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.23	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06789_Aamu_Bax2006_COI	T6-06	1	BC06789_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Present	Sample	Aamu_Bax2006_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	GCACAACCGGGATCTTTACTTC	147.34153
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06789_Pcan_Bot2011_IGS	T6-06	1	BC06789_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-06	IGS	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	CGTAATCCTCAGTACTGGCTA	147.34153
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06789_Sspa_Oph2007_COI	T6-06	1	BC06789_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	GAAGGCCTTCCGATGATC	147.34153
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06789_Upin_Bot2011_COI	T6-06	1	BC06789_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Absent	Sample	Upin_Bot2011_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.34153
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06790_Cmae_Bot2011_COI	T6-07	1	BC06790_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	ATGAACAGTCTATCCTCCTTTAG	147.33483
BC06790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88585	WGS84	BC06790	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06790_Mros_Gib2022_COI	T6-07	1	BC06790_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06790	Sample	BC06790	targeted	Absent	Sample	Mros_Gib2022_COI	T6-07	COI	45	BC06790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33483
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.334	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06792_Aamu_Bax2006_COI	T6-09	1	BC06792_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Present	Sample	Aamu_Bax2006_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	GCACAACCGGGATCTTTACTTC	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06792_Pcan_Bot2011_IGS	T6-09	1	BC06792_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-09	IGS	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	CGTAATCCTCAGTACTGGCTA	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06792_Sspa_Oph2007_COI	T6-09	1	BC06792_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	GAAGGCCTTCCGATGATC	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.204	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06792_Upin_Bot2011_COI	T6-09	1	BC06792_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Present	Sample	Upin_Bot2011_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.33618
BC06793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9627	WGS84	BC06793	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06793_Cjap_Sim2018_COI	T6-10	1	BC06793_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06793	Sample	BC06793	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-10	COI	45	BC06793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53935
BC06793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9627	WGS84	BC06793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.417	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	26/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06793_Mgig_Bot2012_COI	T6-10	1	BC06793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06793	Sample	BC06793	targeted	Present	Sample	Mgig_Bot2012_COI	T6-10	COI	45	BC06793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.53935
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.443	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06794_Cmae_Bot2011_COI	T6-11	1	BC06794_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Present	Sample	Cmae_Bot2011_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	ATGAACAGTCTATCCTCCTTTAG	147.53139
BC06794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96066	WGS84	BC06794	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.167	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06794_Mros_Gib2022_COI	T6-11	1	BC06794_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06794	Sample	BC06794	targeted	Present	Sample	Mros_Gib2022_COI	T6-11	COI	45	BC06794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-11	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53139
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06796_Aamu_Bax2006_COI	T6-13	1	BC06796_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Absent	Sample	Aamu_Bax2006_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	GCACAACCGGGATCTTTACTTC	147.81327
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06796_Pcan_Bot2011_IGS	T6-13	1	BC06796_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-13	IGS	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	CGTAATCCTCAGTACTGGCTA	147.81327
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06796_Sspa_Oph2007_COI	T6-13	1	BC06796_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	GAAGGCCTTCCGATGATC	147.81327
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.129	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06796_Upin_Bot2011_COI	T6-13	1	BC06796_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Present	Sample	Upin_Bot2011_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.81327
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06797_Cjap_Sim2018_COI	T6-14	1	BC06797_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.8762
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	39.213	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	28/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06797_Mgig_Bot2012_COI	T6-14	1	BC06797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Present	Sample	Mgig_Bot2012_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.8762
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06797_Mjap_Gib2022_COI	T6-14	1	BC06797_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	AATTTAATTGTGACTAGGCATGGTCTG	147.8762
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.615	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06798_Cmae_Bot2011_COI	T6-15	1	BC06798_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Present	Sample	Cmae_Bot2011_COI	T6-15	COI	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	ATGAACAGTCTATCCTCCTTTAG	147.87386
BC06798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85089	WGS84	BC06798	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.773	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06798_Mros_Gib2022_COI	T6-15	1	BC06798_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06798	Sample	BC06798	targeted	Present	Sample	Mros_Gib2022_COI	T6-15	COI	45	BC06798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-15	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87386
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.37	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06800_Aamu_Bax2006_COI	T7-01	1	BC06800_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Present	Sample	Aamu_Bax2006_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	GCACAACCGGGATCTTTACTTC	147.81365
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06800_Pcan_Bot2011_IGS	T7-01	1	BC06800_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-01	IGS	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	CGTAATCCTCAGTACTGGCTA	147.81365
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06800_Sspa_Oph2007_COI	T7-01	1	BC06800_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	GAAGGCCTTCCGATGATC	147.81365
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.358	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06800_Upin_Bot2011_COI	T7-01	1	BC06800_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Present	Sample	Upin_Bot2011_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.81365
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06801_Cjap_Sim2018_COI	T7-02	1	BC06801_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87573
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06801_Mgig_Bot2012_COI	T7-02	1	BC06801_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Absent	Sample	Mgig_Bot2012_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.87573
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06801_Mjap_Gib2022_COI	T7-02	1	BC06801_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	AATTTAATTGTGACTAGGCATGGTCTG	147.87573
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06802_Cmae_Bot2011_COI	T7-03	1	BC06802_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Absent	Sample	Cmae_Bot2011_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	ATGAACAGTCTATCCTCCTTTAG	147.87392
BC06802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06802	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.734	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06802_Mros_Gib2022_COI	T7-03	1	BC06802_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06802	Sample	BC06802	targeted	Present	Sample	Mros_Gib2022_COI	T7-03	COI	45	BC06802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87392
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.285	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06804_Aamu_Bax2006_COI	T7-05	1	BC06804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Present	Sample	Aamu_Bax2006_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	GCACAACCGGGATCTTTACTTC	147.30842
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06804_Pcan_Bot2011_IGS	T7-05	1	BC06804_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-05	IGS	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	CGTAATCCTCAGTACTGGCTA	147.30842
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06804_Sspa_Oph2007_COI	T7-05	1	BC06804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	GAAGGCCTTCCGATGATC	147.30842
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06804_Upin_Bot2011_COI	T7-05	1	BC06804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Absent	Sample	Upin_Bot2011_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.30842
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06805_Cjap_Sim2018_COI	T7-06	1	BC06805_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30286
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06805_Mgig_Bot2012_COI	T7-06	1	BC06805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Absent	Sample	Mgig_Bot2012_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.30286
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06805_Mjap_Gib2022_COI	T7-06	1	BC06805_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	AATTTAATTGTGACTAGGCATGGTCTG	147.30286
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.285	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06806_Cmae_Bot2011_COI	T7-07	1	BC06806_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Present	Sample	Cmae_Bot2011_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	ATGAACAGTCTATCCTCCTTTAG	147.30118
BC06806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82894	WGS84	BC06806	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06806_Mros_Gib2022_COI	T7-07	1	BC06806_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06806	Sample	BC06806	targeted	Absent	Sample	Mros_Gib2022_COI	T7-07	COI	45	BC06806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30118
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.005	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06808_Aamu_Bax2006_COI	T7-09	1	BC06808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Present	Sample	Aamu_Bax2006_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	GCACAACCGGGATCTTTACTTC	147.34178
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06808_Pcan_Bot2011_IGS	T7-09	1	BC06808_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-09	IGS	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	CGTAATCCTCAGTACTGGCTA	147.34178
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06808_Sspa_Oph2007_COI	T7-09	1	BC06808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	GAAGGCCTTCCGATGATC	147.34178
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06808_Upin_Bot2011_COI	T7-09	1	BC06808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Absent	Sample	Upin_Bot2011_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.34178
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06809_Cjap_Sim2018_COI	T7-10	1	BC06809_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34175
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06809_Mgig_Bot2012_COI	T7-10	1	BC06809_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Absent	Sample	Mgig_Bot2012_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.34175
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06809_Mjap_Gib2022_COI	T7-10	1	BC06809_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	AATTTAATTGTGACTAGGCATGGTCTG	147.34175
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.222	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06811_Aamu_Bax2006_COI	T7-12	1	BC06811_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Present	Sample	Aamu_Bax2006_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	GCACAACCGGGATCTTTACTTC	147.33607
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06811_Pcan_Bot2011_IGS	T7-12	1	BC06811_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-12	IGS	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	CGTAATCCTCAGTACTGGCTA	147.33607
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06811_Sspa_Oph2007_COI	T7-12	1	BC06811_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	GAAGGCCTTCCGATGATC	147.33607
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06811_Upin_Bot2011_COI	T7-12	1	BC06811_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Absent	Sample	Upin_Bot2011_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.33607
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06812_Cjap_Sim2018_COI	T7-13	1	BC06812_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33619
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.313	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06812_Mgig_Bot2012_COI	T7-13	1	BC06812_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Present	Sample	Mgig_Bot2012_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.33619
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06812_Mjap_Gib2022_COI	T7-13	1	BC06812_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	AATTTAATTGTGACTAGGCATGGTCTG	147.33619
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.396	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06813_Cmae_Bot2011_COI	T7-14	1	BC06813_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Present	Sample	Cmae_Bot2011_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	ATGAACAGTCTATCCTCCTTTAG	147.53937
BC06813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96293	WGS84	BC06813	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	26.672	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06813_Mros_Gib2022_COI	T7-14	1	BC06813_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06813	Sample	BC06813	targeted	Present	Sample	Mros_Gib2022_COI	T7-14	COI	45	BC06813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-14	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53937
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.888	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06815_Aamu_Bax2006_COI	T7-16	1	BC06815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Present	Sample	Aamu_Bax2006_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	GCACAACCGGGATCTTTACTTC	147.51962
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06815_Pcan_Bot2011_IGS	T7-16	1	BC06815_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-16	IGS	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	CGTAATCCTCAGTACTGGCTA	147.51962
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06815_Sspa_Oph2007_COI	T7-16	1	BC06815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	GAAGGCCTTCCGATGATC	147.51962
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06815_Upin_Bot2011_COI	T7-16	1	BC06815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Absent	Sample	Upin_Bot2011_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.51962
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06816_Cjap_Sim2018_COI	T8-01	1	BC06816_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81263
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.559	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	17/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06816_Mgig_Bot2012_COI	T8-01	1	BC06816_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Present	Sample	Mgig_Bot2012_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.81263
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06816_Mjap_Gib2022_COI	T8-01	1	BC06816_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	AATTTAATTGTGACTAGGCATGGTCTG	147.81263
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.81	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06817_Cmae_Bot2011_COI	T8-02	1	BC06817_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Present	Sample	Cmae_Bot2011_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	ATGAACAGTCTATCCTCCTTTAG	147.87661
BC06817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84723	WGS84	BC06817	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	31.134	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06817_Mros_Gib2022_COI	T8-02	1	BC06817_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06817	Sample	BC06817	targeted	Present	Sample	Mros_Gib2022_COI	T8-02	COI	45	BC06817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87661
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06819_Aamu_Bax2006_COI	T8-04	1	BC06819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Absent	Sample	Aamu_Bax2006_COI	T8-04	COI	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	GCACAACCGGGATCTTTACTTC	147.85574
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06819_Pcan_Bot2011_IGS	T8-04	1	BC06819_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-04	IGS	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	CGTAATCCTCAGTACTGGCTA	147.85574
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06819_Sspa_Oph2007_COI	T8-04	1	BC06819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-04	COI	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	GAAGGCCTTCCGATGATC	147.85574
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.688	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06819_Upin_Bot2011_COI	T8-04	1	BC06819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Present	Sample	Upin_Bot2011_COI	T8-04	COI	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.85574
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.072	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06820_Cmae_Bot2011_COI	T8-05	1	BC06820_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Present	Sample	Cmae_Bot2011_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	ATGAACAGTCTATCCTCCTTTAG	147.81282
BC06820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88955	WGS84	BC06820	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.733	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06820_Mros_Gib2022_COI	T8-05	1	BC06820_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06820	Sample	BC06820	targeted	Present	Sample	Mros_Gib2022_COI	T8-05	COI	45	BC06820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81282
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06822_Aamu_Bax2006_COI	T8-07	1	BC06822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Aamu_Bax2006_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	GCACAACCGGGATCTTTACTTC	147.30214
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06822_Pcan_Bot2011_IGS	T8-07	1	BC06822_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-07	IGS	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	CGTAATCCTCAGTACTGGCTA	147.30214
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06822_Sspa_Oph2007_COI	T8-07	1	BC06822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	GAAGGCCTTCCGATGATC	147.30214
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06822_Upin_Bot2011_COI	T8-07	1	BC06822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Upin_Bot2011_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.30214
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06823_Cjap_Sim2018_COI	T8-08	1	BC06823_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30793
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.209	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06823_Mgig_Bot2012_COI	T8-08	1	BC06823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Present	Sample	Mgig_Bot2012_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.30793
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06823_Mjap_Gib2022_COI	T8-08	1	BC06823_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	AATTTAATTGTGACTAGGCATGGTCTG	147.30793
BC06824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87309	WGS84	BC06824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06824_Cmae_Bot2011_COI	T8-09	1	BC06824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06824	Sample	BC06824	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-09	COI	45	BC06824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-09	ATGAACAGTCTATCCTCCTTTAG	147.33661
BC06826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88087	WGS84	BC06826	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.57	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06826_Aamu_Bax2006_COI	T8-11	1	BC06826_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06826	Sample	BC06826	targeted	Present	Sample	Aamu_Bax2006_COI	T8-11	COI	45	BC06826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-11	GCACAACCGGGATCTTTACTTC	147.34167
BC06826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88087	WGS84	BC06826	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06826_Pcan_Bot2011_IGS	T8-11	1	BC06826_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06826	Sample	BC06826	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-11	IGS	45	BC06826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-11	CGTAATCCTCAGTACTGGCTA	147.34167
BC06826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88087	WGS84	BC06826	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06826_Sspa_Oph2007_COI	T8-11	1	BC06826_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06826	Sample	BC06826	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-11	COI	45	BC06826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-11	GAAGGCCTTCCGATGATC	147.34167
BC06826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88087	WGS84	BC06826	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06826_Upin_Bot2011_COI	T8-11	1	BC06826_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06826	Sample	BC06826	targeted	Absent	Sample	Upin_Bot2011_COI	T8-11	COI	45	BC06826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.34167
BC06827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88574	WGS84	BC06827	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06827_Cjap_Sim2018_COI	T8-12	1	BC06827_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06827	Sample	BC06827	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-12	COI	45	BC06827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3353
BC06827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88574	WGS84	BC06827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.482	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06827_Mgig_Bot2012_COI	T8-12	1	BC06827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06827	Sample	BC06827	targeted	Present	Sample	Mgig_Bot2012_COI	T8-12	COI	45	BC06827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.3353
BC06828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88425	WGS84	BC06828	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06828_Cmae_Bot2011_COI	T8-13	1	BC06828_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06828	Sample	BC06828	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-13	COI	45	BC06828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-13	ATGAACAGTCTATCCTCCTTTAG	147.33592
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06830_Cjap_Sim2018_COI	T8-15	1	BC06830_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53978
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	20.955	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	28/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06830_Mgig_Bot2012_COI	T8-15	1	BC06830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Present	Sample	Mgig_Bot2012_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.53978
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06830_Mjap_Gib2022_COI	T8-15	1	BC06830_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	AATTTAATTGTGACTAGGCATGGTCTG	147.53978
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.901	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06831_Cmae_Bot2011_COI	T8-16	1	BC06831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Present	Sample	Cmae_Bot2011_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	ATGAACAGTCTATCCTCCTTTAG	147.51943
BC06831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9619	WGS84	BC06831	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	30.013	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06831_Mros_Gib2022_COI	T8-16	1	BC06831_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06831	Sample	BC06831	targeted	Present	Sample	Mros_Gib2022_COI	T8-16	COI	45	BC06831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-16	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.51943
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.894	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06833_Aamu_Bax2006_COI	T8-18	1	BC06833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Present	Sample	Aamu_Bax2006_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	GCACAACCGGGATCTTTACTTC	147.53883
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06833_Pcan_Bot2011_IGS	T8-18	1	BC06833_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-18	IGS	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	CGTAATCCTCAGTACTGGCTA	147.53883
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06833_Sspa_Oph2007_COI	T8-18	1	BC06833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	GAAGGCCTTCCGATGATC	147.53883
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06833_Upin_Bot2011_COI	T8-18	1	BC06833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Absent	Sample	Upin_Bot2011_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	CTTTAATTACAGCGTTTTTATTGTTGT	147.53883
BC11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84564	WGS84	BC11605	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.424	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11605_Mgig_Bot2012_COI	S10-04	1	BC11605_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11605	Sample	BC11605	targeted	Present	Sample	Mgig_Bot2012_COI	S10-04	COI	45	BC11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49687
BC11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78555	WGS84	BC11609	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.678	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11609_Mgig_Bot2012_COI	S10-12	1	BC11609_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11609	Sample	BC11609	targeted	Present	Sample	Mgig_Bot2012_COI	S10-12	COI	45	BC11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.51518
BC11612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80969	WGS84	BC11612	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.985	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11612_Mgig_Bot2012_COI	S10-07	1	BC11612_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11612	Sample	BC11612	targeted	Present	Sample	Mgig_Bot2012_COI	S10-07	COI	45	BC11612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51882
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.412	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11615_Aamu_Bax2006_COI	T16-01	1	BC11615_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Present	Sample	Aamu_Bax2006_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	GCACAACCGGGATCTTTACTTC	147.53922
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11615_Pcan_Bot2011_IGS	T16-01	1	BC11615_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-01	IGS	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	CGTAATCCTCAGTACTGGCTA	147.53922
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11615_Sspa_Oph2007_COI	T16-01	1	BC11615_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	GAAGGCCTTCCGATGATC	147.53922
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11615_Upin_Bot2011_COI	T16-01	1	BC11615_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Absent	Sample	Upin_Bot2011_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.53922
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11616_Cjap_Sim2018_COI	T16-02	1	BC11616_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52991
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11616_Mgig_Bot2012_COI	T16-02	1	BC11616_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Absent	Sample	Mgig_Bot2012_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.52991
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11616_Mjap_Gib2022_COI	T16-02	1	BC11616_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	AATTTAATTGTGACTAGGCATGGTCTG	147.52991
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.861	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11617_Cmae_Bot2011_COI	T16-03	1	BC11617_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Present	Sample	Cmae_Bot2011_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	ATGAACAGTCTATCCTCCTTTAG	147.52225
BC11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96038	WGS84	BC11617	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11617_Mros_Gib2022_COI	T16-03	1	BC11617_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11617	Sample	BC11617	targeted	Absent	Sample	Mros_Gib2022_COI	T16-03	COI	45	BC11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.52225
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.712	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11619_Aamu_Bax2006_COI	T16-05	1	BC11619_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Present	Sample	Aamu_Bax2006_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	GCACAACCGGGATCTTTACTTC	147.46533
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11619_Pcan_Bot2011_IGS	T16-05	1	BC11619_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-05	IGS	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	CGTAATCCTCAGTACTGGCTA	147.46533
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11619_Sspa_Oph2007_COI	T16-05	1	BC11619_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	GAAGGCCTTCCGATGATC	147.46533
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11619_Upin_Bot2011_COI	T16-05	1	BC11619_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Upin_Bot2011_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.46533
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11620_Cmae_Bot2011_COI	T16-06	1	BC11620_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Cmae_Bot2011_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	ATGAACAGTCTATCCTCCTTTAG	147.48517
BC11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80249	WGS84	BC11620	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11620_Mros_Gib2022_COI	T16-06	1	BC11620_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11620	Sample	BC11620	targeted	Absent	Sample	Mros_Gib2022_COI	T16-06	COI	45	BC11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.48517
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.261	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11622_Aamu_Bax2006_COI	T16-08	1	BC11622_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Present	Sample	Aamu_Bax2006_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	GCACAACCGGGATCTTTACTTC	147.52327
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11622_Pcan_Bot2011_IGS	T16-08	1	BC11622_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-08	IGS	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	CGTAATCCTCAGTACTGGCTA	147.52327
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11622_Sspa_Oph2007_COI	T16-08	1	BC11622_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	GAAGGCCTTCCGATGATC	147.52327
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11622_Upin_Bot2011_COI	T16-08	1	BC11622_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Absent	Sample	Upin_Bot2011_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.52327
BC11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83598	WGS84	BC11623	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.429	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11623_Mgig_Bot2012_COI	S11-01	1	BC11623_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11623	Sample	BC11623	targeted	Present	Sample	Mgig_Bot2012_COI	S11-01	COI	45	BC11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.50838
BC11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84605	WGS84	BC11627	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.614	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11627_Mgig_Bot2012_COI	S11-05	1	BC11627_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11627	Sample	BC11627	targeted	Present	Sample	Mgig_Bot2012_COI	S11-05	COI	45	BC11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.49691
BC11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78476	WGS84	BC11630	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.217	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11630_Mgig_Bot2012_COI	S11-08	1	BC11630_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11630	Sample	BC11630	targeted	Present	Sample	Mgig_Bot2012_COI	S11-08	COI	45	BC11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51475
BC11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.81014	WGS84	BC11634	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.715	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11634_Mgig_Bot2012_COI	S11-12	1	BC11634_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11634	Sample	BC11634	targeted	Present	Sample	Mgig_Bot2012_COI	S11-12	COI	45	BC11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.52041
BC11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83648	WGS84	BC11638	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.618	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11638_Mgig_Bot2012_COI	S12-04	1	BC11638_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11638	Sample	BC11638	targeted	Present	Sample	Mgig_Bot2012_COI	S12-04	COI	45	BC11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.50804
BC11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.77982	WGS84	BC11641	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.666	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11641_Mgig_Bot2012_COI	S12-07	1	BC11641_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11641	Sample	BC11641	targeted	Present	Sample	Mgig_Bot2012_COI	S12-07	COI	45	BC11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51772
BC11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.8092	WGS84	BC11645	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.486	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11645_Mgig_Bot2012_COI	S12-11	1	BC11645_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11645	Sample	BC11645	targeted	Present	Sample	Mgig_Bot2012_COI	S12-11	COI	45	BC11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51928
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.801	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11648_Aamu_Bax2006_COI	T17-03	1	BC11648_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Present	Sample	Aamu_Bax2006_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	GCACAACCGGGATCTTTACTTC	147.51982
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11648_Pcan_Bot2011_IGS	T17-03	1	BC11648_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-03	IGS	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	CGTAATCCTCAGTACTGGCTA	147.51982
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11648_Sspa_Oph2007_COI	T17-03	1	BC11648_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	GAAGGCCTTCCGATGATC	147.51982
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11648_Upin_Bot2011_COI	T17-03	1	BC11648_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Absent	Sample	Upin_Bot2011_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.51982
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11649_Cjap_Sim2018_COI	T17-04	1	BC11649_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52798
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	11/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11649_Mgig_Bot2012_COI	T17-04	1	BC11649_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Absent	Sample	Mgig_Bot2012_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.52798
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11649_Mjap_Gib2022_COI	T17-04	1	BC11649_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	AATTTAATTGTGACTAGGCATGGTCTG	147.52798
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11651_Aamu_Bax2006_COI	T17-07	1	BC11651_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Absent	Sample	Aamu_Bax2006_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	GCACAACCGGGATCTTTACTTC	147.50293
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11651_Pcan_Bot2011_IGS	T17-07	1	BC11651_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-07	IGS	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	CGTAATCCTCAGTACTGGCTA	147.50293
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11651_Sspa_Oph2007_COI	T17-07	1	BC11651_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	GAAGGCCTTCCGATGATC	147.50293
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11651_Upin_Bot2011_COI	T17-07	1	BC11651_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Absent	Sample	Upin_Bot2011_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.50293
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11652_Cjap_Sim2018_COI	T17-08	1	BC11652_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52213
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.4	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	22/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11652_Mgig_Bot2012_COI	T17-08	1	BC11652_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Present	Sample	Mgig_Bot2012_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.52213
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11652_Mjap_Gib2022_COI	T17-08	1	BC11652_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	AATTTAATTGTGACTAGGCATGGTCTG	147.52213
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.158	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11653_Cmae_Bot2011_COI	T17-02	1	BC11653_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Present	Sample	Cmae_Bot2011_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	ATGAACAGTCTATCCTCCTTTAG	147.52365
BC11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95997	WGS84	BC11653	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	25.58	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11653_Mros_Gib2022_COI	T17-02	1	BC11653_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11653	Sample	BC11653	targeted	Present	Sample	Mros_Gib2022_COI	T17-02	COI	45	BC11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.52365
BC11656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80988	WGS84	BC11656	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.897	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11656_Mgig_Bot2012_COI	S9-11	1	BC11656_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11656	Sample	BC11656	targeted	Present	Sample	Mgig_Bot2012_COI	S9-11	COI	45	BC11656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.52017
BC11663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83685	WGS84	BC11663	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.063	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11663_Mgig_Bot2012_COI	S9-07	1	BC11663_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11663	Sample	BC11663	targeted	Present	Sample	Mgig_Bot2012_COI	S9-07	COI	45	BC11663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.50923
BC11667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96492	WGS84	BC11667	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11667_Cjap_Sim2018_COI	T18-09	1	BC11667_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11667	Sample	BC11667	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-09	COI	45	BC11667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51981
BC11667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96492	WGS84	BC11667	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.551	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	14/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11667_Mgig_Bot2012_COI	T18-09	1	BC11667_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11667	Sample	BC11667	targeted	Present	Sample	Mgig_Bot2012_COI	T18-09	COI	45	BC11667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.51981
BC11670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.8116	WGS84	BC11670	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.101	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11670_Mgig_Bot2012_COI	S9-15	1	BC11670_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11670	Sample	BC11670	targeted	Present	Sample	Mgig_Bot2012_COI	S9-15	COI	45	BC11670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.52303
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11671_Cmae_Bot2011_COI	T15-15	1	BC11671_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	ATGAACAGTCTATCCTCCTTTAG	147.5266
BC11671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95932	WGS84	BC11671	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11671_Mros_Gib2022_COI	T15-15	1	BC11671_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11671	Sample	BC11671	targeted	Absent	Sample	Mros_Gib2022_COI	T15-15	COI	45	BC11671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-15	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.5266
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.021	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11673_Aamu_Bax2006_COI	T15-13	1	BC11673_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Present	Sample	Aamu_Bax2006_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	GCACAACCGGGATCTTTACTTC	147.3371
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11673_Pcan_Bot2011_IGS	T15-13	1	BC11673_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-13	IGS	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	CGTAATCCTCAGTACTGGCTA	147.3371
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11673_Sspa_Oph2007_COI	T15-13	1	BC11673_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	GAAGGCCTTCCGATGATC	147.3371
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.455	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11673_Upin_Bot2011_COI	T15-13	1	BC11673_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Present	Sample	Upin_Bot2011_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.3371
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11674_Cjap_Sim2018_COI	T15-12	1	BC11674_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33631
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11674_Mgig_Bot2012_COI	T15-12	1	BC11674_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.33631
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11674_Mjap_Gib2022_COI	T15-12	1	BC11674_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	AATTTAATTGTGACTAGGCATGGTCTG	147.33631
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.935	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11675_Cmae_Bot2011_COI	T15-11	1	BC11675_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Present	Sample	Cmae_Bot2011_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	ATGAACAGTCTATCCTCCTTTAG	147.33592
BC11675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88575	WGS84	BC11675	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11675_Mros_Gib2022_COI	T15-11	1	BC11675_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11675	Sample	BC11675	targeted	Absent	Sample	Mros_Gib2022_COI	T15-11	COI	45	BC11675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-11	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33592
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.058	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11677_Aamu_Bax2006_COI	T15-09	1	BC11677_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Present	Sample	Aamu_Bax2006_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	GCACAACCGGGATCTTTACTTC	147.34172
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11677_Pcan_Bot2011_IGS	T15-09	1	BC11677_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-09	IGS	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	CGTAATCCTCAGTACTGGCTA	147.34172
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11677_Sspa_Oph2007_COI	T15-09	1	BC11677_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	GAAGGCCTTCCGATGATC	147.34172
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11677_Upin_Bot2011_COI	T15-09	1	BC11677_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Absent	Sample	Upin_Bot2011_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.34172
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11678_Cjap_Sim2018_COI	T15-08	1	BC11678_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33654
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11678_Mgig_Bot2012_COI	T15-08	1	BC11678_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.33654
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11678_Mjap_Gib2022_COI	T15-08	1	BC11678_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	AATTTAATTGTGACTAGGCATGGTCTG	147.33654
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11679_Cmae_Bot2011_COI	T15-07	1	BC11679_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	ATGAACAGTCTATCCTCCTTTAG	147.30774
BC11679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82533	WGS84	BC11679	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11679_Mros_Gib2022_COI	T15-07	1	BC11679_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11679	Sample	BC11679	targeted	Absent	Sample	Mros_Gib2022_COI	T15-07	COI	45	BC11679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30774
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11680_Aamu_Bax2006_COI	T15-06	1	BC11680_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Aamu_Bax2006_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	GCACAACCGGGATCTTTACTTC	147.30245
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11680_Pcan_Bot2011_IGS	T15-06	1	BC11680_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-06	IGS	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	CGTAATCCTCAGTACTGGCTA	147.30245
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11680_Sspa_Oph2007_COI	T15-06	1	BC11680_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	GAAGGCCTTCCGATGATC	147.30245
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11680_Upin_Bot2011_COI	T15-06	1	BC11680_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Upin_Bot2011_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.30245
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11681_Cjap_Sim2018_COI	T15-05	1	BC11681_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30244
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11681_Mgig_Bot2012_COI	T15-05	1	BC11681_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.30244
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11681_Mjap_Gib2022_COI	T15-05	1	BC11681_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	AATTTAATTGTGACTAGGCATGGTCTG	147.30244
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11682_Cmae_Bot2011_COI	T15-04	1	BC11682_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	ATGAACAGTCTATCCTCCTTTAG	147.81383
BC11682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88946	WGS84	BC11682	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11682_Mros_Gib2022_COI	T15-04	1	BC11682_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11682	Sample	BC11682	targeted	Absent	Sample	Mros_Gib2022_COI	T15-04	COI	45	BC11682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81383
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.203	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11684_Aamu_Bax2006_COI	T15-02	1	BC11684_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Present	Sample	Aamu_Bax2006_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	GCACAACCGGGATCTTTACTTC	147.87397
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11684_Pcan_Bot2011_IGS	T15-02	1	BC11684_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-02	IGS	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	CGTAATCCTCAGTACTGGCTA	147.87397
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11684_Sspa_Oph2007_COI	T15-02	1	BC11684_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	GAAGGCCTTCCGATGATC	147.87397
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.352	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11684_Upin_Bot2011_COI	T15-02	1	BC11684_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Present	Sample	Upin_Bot2011_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.87397
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11685_Cjap_Sim2018_COI	T15-01	1	BC11685_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87463
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	24/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11685_Mgig_Bot2012_COI	T15-01	1	BC11685_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.87463
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11685_Mjap_Gib2022_COI	T15-01	1	BC11685_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	AATTTAATTGTGACTAGGCATGGTCTG	147.87463
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11686_Cmae_Bot2011_COI	T14-11	1	BC11686_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Cmae_Bot2011_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	ATGAACAGTCTATCCTCCTTTAG	147.34177
BC11686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88122	WGS84	BC11686	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11686_Mros_Gib2022_COI	T14-11	1	BC11686_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11686	Sample	BC11686	targeted	Absent	Sample	Mros_Gib2022_COI	T14-11	COI	45	BC11686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-11	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34177
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.983	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11688_Aamu_Bax2006_COI	T14-14	1	BC11688_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Present	Sample	Aamu_Bax2006_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	GCACAACCGGGATCTTTACTTC	147.53562
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11688_Pcan_Bot2011_IGS	T14-14	1	BC11688_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-14	IGS	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	CGTAATCCTCAGTACTGGCTA	147.53562
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11688_Sspa_Oph2007_COI	T14-14	1	BC11688_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	GAAGGCCTTCCGATGATC	147.53562
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.429	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11688_Upin_Bot2011_COI	T14-14	1	BC11688_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Present	Sample	Upin_Bot2011_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.53562
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11689_Cjap_Sim2018_COI	T14-13	1	BC11689_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33753
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11689_Mgig_Bot2012_COI	T14-13	1	BC11689_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.33753
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11689_Mjap_Gib2022_COI	T14-13	1	BC11689_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	AATTTAATTGTGACTAGGCATGGTCTG	147.33753
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.739	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11691_Aamu_Bax2006_COI	T14-16	1	BC11691_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Present	Sample	Aamu_Bax2006_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	GCACAACCGGGATCTTTACTTC	147.52389
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11691_Pcan_Bot2011_IGS	T14-16	1	BC11691_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-16	IGS	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	CGTAATCCTCAGTACTGGCTA	147.52389
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11691_Sspa_Oph2007_COI	T14-16	1	BC11691_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	GAAGGCCTTCCGATGATC	147.52389
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11691_Upin_Bot2011_COI	T14-16	1	BC11691_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Absent	Sample	Upin_Bot2011_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.52389
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11692_Cjap_Sim2018_COI	T14-10	1	BC11692_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33662
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11692_Mgig_Bot2012_COI	T14-10	1	BC11692_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.33662
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11692_Mjap_Gib2022_COI	T14-10	1	BC11692_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	AATTTAATTGTGACTAGGCATGGTCTG	147.33662
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11695_Aamu_Bax2006_COI	T14-07	1	BC11695_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Aamu_Bax2006_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	GCACAACCGGGATCTTTACTTC	147.30768
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11695_Pcan_Bot2011_IGS	T14-07	1	BC11695_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-07	IGS	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	CGTAATCCTCAGTACTGGCTA	147.30768
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11695_Sspa_Oph2007_COI	T14-07	1	BC11695_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	GAAGGCCTTCCGATGATC	147.30768
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11695_Upin_Bot2011_COI	T14-07	1	BC11695_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Upin_Bot2011_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.30768
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11696_Cjap_Sim2018_COI	T14-06	1	BC11696_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3019
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11696_Mgig_Bot2012_COI	T14-06	1	BC11696_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.3019
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11696_Mjap_Gib2022_COI	T14-06	1	BC11696_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	AATTTAATTGTGACTAGGCATGGTCTG	147.3019
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11697_Cmae_Bot2011_COI	T14-05	1	BC11697_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Cmae_Bot2011_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	ATGAACAGTCTATCCTCCTTTAG	147.30227
BC11697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82933	WGS84	BC11697	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11697_Mros_Gib2022_COI	T14-05	1	BC11697_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11697	Sample	BC11697	targeted	Absent	Sample	Mros_Gib2022_COI	T14-05	COI	45	BC11697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30227
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.613	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11699_Aamu_Bax2006_COI	T14-03	1	BC11699_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Present	Sample	Aamu_Bax2006_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	GCACAACCGGGATCTTTACTTC	147.87279
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11699_Pcan_Bot2011_IGS	T14-03	1	BC11699_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-03	IGS	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	CGTAATCCTCAGTACTGGCTA	147.87279
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11699_Sspa_Oph2007_COI	T14-03	1	BC11699_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	GAAGGCCTTCCGATGATC	147.87279
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.647	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11699_Upin_Bot2011_COI	T14-03	1	BC11699_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Present	Sample	Upin_Bot2011_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.87279
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11700_Cjap_Sim2018_COI	T14-02	1	BC11700_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87373
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	26/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11700_Mgig_Bot2012_COI	T14-02	1	BC11700_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.87373
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11700_Mjap_Gib2022_COI	T14-02	1	BC11700_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	AATTTAATTGTGACTAGGCATGGTCTG	147.87373
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.264	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11701_Cmae_Bot2011_COI	T14-01	1	BC11701_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Present	Sample	Cmae_Bot2011_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	ATGAACAGTCTATCCTCCTTTAG	147.87673
BC11701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84678	WGS84	BC11701	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11701_Mros_Gib2022_COI	T14-01	1	BC11701_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11701	Sample	BC11701	targeted	Absent	Sample	Mros_Gib2022_COI	T14-01	COI	45	BC11701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87673
BC15784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88221	WGS84	BC15784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15784_Mgig_Bot2012_COI	HA1	0	BC15784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15784	Sample	BC15784	targeted	Absent	Sample	Mgig_Bot2012_COI	HA1	COI	45	BC15784	1.00E-05	Nil	Australia	HA1	TCTTATTCGTTGGAGACTTTATAACCCT	147.34465
BC15785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88364	WGS84	BC15785	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15785_Cmae_Bot2011_COI	HA11	0	BC15785_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15785	Sample	BC15785	targeted	Absent	Sample	Cmae_Bot2011_COI	HA11	COI	45	BC15785	1.00E-05	Nil	Australia	HA11	ATGAACAGTCTATCCTCCTTTAG	147.33641
BC15787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84498	WGS84	BC15787	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.448	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15787_Aamu_Bax2006_COI	HA19	0	BC15787_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15787	Sample	BC15787	targeted	Present	Sample	Aamu_Bax2006_COI	HA19	COI	45	BC15787	1.00E-05	Nil	Australia	HA19	GCACAACCGGGATCTTTACTTC	147.32942
BC15787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84498	WGS84	BC15787	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15787_Sspa_Oph2007_COI	HA19	0	BC15787_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15787	Sample	BC15787	targeted	Absent	Sample	Sspa_Oph2007_COI	HA19	COI	45	BC15787	1.00E-05	Nil	Australia	HA19	GAAGGCCTTCCGATGATC	147.32942
BC15787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84498	WGS84	BC15787	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15787_Upin_Bot2011_COI	HA19	0	BC15787_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15787	Sample	BC15787	targeted	Absent	Sample	Upin_Bot2011_COI	HA19	COI	45	BC15787	1.00E-05	Nil	Australia	HA19	CTTTAATTACAGCGTTTTTATTGTTGT	147.32942
BC15788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84393	WGS84	BC15788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15788_Mgig_Bot2012_COI	HA21	0	BC15788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15788	Sample	BC15788	targeted	Absent	Sample	Mgig_Bot2012_COI	HA21	COI	45	BC15788	1.00E-05	Nil	Australia	HA21	TCTTATTCGTTGGAGACTTTATAACCCT	147.32811
BC15789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83078	WGS84	BC15789	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15789_Cmae_Bot2011_COI	HA23	0	BC15789_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15789	Sample	BC15789	targeted	Absent	Sample	Cmae_Bot2011_COI	HA23	COI	45	BC15789	1.00E-05	Nil	Australia	HA23	ATGAACAGTCTATCCTCCTTTAG	147.32042
BC15790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88608	WGS84	BC15790	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.827	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15790_Aamu_Bax2006_COI	HA3	0	BC15790_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15790	Sample	BC15790	targeted	Present	Sample	Aamu_Bax2006_COI	HA3	COI	45	BC15790	1.00E-05	Nil	Australia	HA3	GCACAACCGGGATCTTTACTTC	147.33912
BC15790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88608	WGS84	BC15790	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15790_Sspa_Oph2007_COI	HA3	0	BC15790_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15790	Sample	BC15790	targeted	Absent	Sample	Sspa_Oph2007_COI	HA3	COI	45	BC15790	1.00E-05	Nil	Australia	HA3	GAAGGCCTTCCGATGATC	147.33912
BC15790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88608	WGS84	BC15790	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15790_Upin_Bot2011_COI	HA3	0	BC15790_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15790	Sample	BC15790	targeted	Absent	Sample	Upin_Bot2011_COI	HA3	COI	45	BC15790	1.00E-05	Nil	Australia	HA3	CTTTAATTACAGCGTTTTTATTGTTGT	147.33912
BC15791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18983	WGS84	BC15791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15791_Mgig_Bot2012_COI	DD2	0	BC15791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15791	Sample	BC15791	targeted	Absent	Sample	Mgig_Bot2012_COI	DD2	COI	45	BC15791	1.00E-05	Nil	Australia	DD2	TCTTATTCGTTGGAGACTTTATAACCCT	146.36787
BC15792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18675	WGS84	BC15792	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15792_Cmae_Bot2011_COI	DD7	0	BC15792_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15792	Sample	BC15792	targeted	Absent	Sample	Cmae_Bot2011_COI	DD7	COI	45	BC15792	1.00E-05	Nil	Australia	DD7	ATGAACAGTCTATCCTCCTTTAG	146.36488
BC15794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18373	WGS84	BC15794	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15794_Aamu_Bax2006_COI	DD14	0	BC15794_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15794	Sample	BC15794	targeted	Absent	Sample	Aamu_Bax2006_COI	DD14	COI	45	BC15794	1.00E-05	Nil	Australia	DD14	GCACAACCGGGATCTTTACTTC	146.3646
BC15794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18373	WGS84	BC15794	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15794_Sspa_Oph2007_COI	DD14	0	BC15794_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15794	Sample	BC15794	targeted	Absent	Sample	Sspa_Oph2007_COI	DD14	COI	45	BC15794	1.00E-05	Nil	Australia	DD14	GAAGGCCTTCCGATGATC	146.3646
BC15794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18373	WGS84	BC15794	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15794_Upin_Bot2011_COI	DD14	0	BC15794_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15794	Sample	BC15794	targeted	Absent	Sample	Upin_Bot2011_COI	DD14	COI	45	BC15794	1.00E-05	Nil	Australia	DD14	CTTTAATTACAGCGTTTTTATTGTTGT	146.3646
BC15795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84704	WGS84	BC15795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15795_Mgig_Bot2012_COI	MB27	0	BC15795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15795	Sample	BC15795	targeted	Absent	Sample	Mgig_Bot2012_COI	MB27	COI	45	BC15795	1.00E-05	Nil	Australia	MB27	TCTTATTCGTTGGAGACTTTATAACCCT	144.9319
BC15796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8438	WGS84	BC15796	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15796_Cmae_Bot2011_COI	MB24	0	BC15796_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15796	Sample	BC15796	targeted	Absent	Sample	Cmae_Bot2011_COI	MB24	COI	45	BC15796	1.00E-05	Nil	Australia	MB24	ATGAACAGTCTATCCTCCTTTAG	144.92894
BC15798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84384	WGS84	BC15798	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.877	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15798_Aamu_Bax2006_COI	MB19	0	BC15798_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15798	Sample	BC15798	targeted	Present	Sample	Aamu_Bax2006_COI	MB19	COI	45	BC15798	1.00E-05	Nil	Australia	MB19	GCACAACCGGGATCTTTACTTC	144.92641
BC15798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84384	WGS84	BC15798	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.802	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15798_Sspa_Oph2007_COI	MB19	0	BC15798_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15798	Sample	BC15798	targeted	Present	Sample	Sspa_Oph2007_COI	MB19	COI	45	BC15798	1.00E-05	Nil	Australia	MB19	GAAGGCCTTCCGATGATC	144.92641
BC15798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84384	WGS84	BC15798	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.616	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15798_Upin_Bot2011_COI	MB19	0	BC15798_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15798	Sample	BC15798	targeted	Present	Sample	Upin_Bot2011_COI	MB19	COI	45	BC15798	1.00E-05	Nil	Australia	MB19	CTTTAATTACAGCGTTTTTATTGTTGT	144.92641
BC15799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85028	WGS84	BC15799	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15799_Mgig_Bot2012_COI	MB17	0	BC15799_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15799	Sample	BC15799	targeted	Absent	Sample	Mgig_Bot2012_COI	MB17	COI	45	BC15799	1.00E-05	Nil	Australia	MB17	TCTTATTCGTTGGAGACTTTATAACCCT	144.91393
BC15800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84782	WGS84	BC15800	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15800_Cmae_Bot2011_COI	MB14	0	BC15800_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15800	Sample	BC15800	targeted	Absent	Sample	Cmae_Bot2011_COI	MB14	COI	45	BC15800	1.00E-05	Nil	Australia	MB14	ATGAACAGTCTATCCTCCTTTAG	144.91026
BC15802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82346	WGS84	BC15802	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15802_Aamu_Bax2006_COI	MB122	0	BC15802_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15802	Sample	BC15802	targeted	Absent	Sample	Aamu_Bax2006_COI	MB122	COI	45	BC15802	1.00E-05	Nil	Australia	MB122	GCACAACCGGGATCTTTACTTC	144.90165
BC15802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82346	WGS84	BC15802	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.544	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15802_Sspa_Oph2007_COI	MB122	0	BC15802_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15802	Sample	BC15802	targeted	Present	Sample	Sspa_Oph2007_COI	MB122	COI	45	BC15802	1.00E-05	Nil	Australia	MB122	GAAGGCCTTCCGATGATC	144.90165
BC15802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82346	WGS84	BC15802	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15802_Upin_Bot2011_COI	MB122	0	BC15802_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15802	Sample	BC15802	targeted	Absent	Sample	Upin_Bot2011_COI	MB122	COI	45	BC15802	1.00E-05	Nil	Australia	MB122	CTTTAATTACAGCGTTTTTATTGTTGT	144.90165
BC15803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82012	WGS84	BC15803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.672	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15803_Mgig_Bot2012_COI	MB118	0	BC15803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15803	Sample	BC15803	targeted	Present	Sample	Mgig_Bot2012_COI	MB118	COI	45	BC15803	1.00E-05	Nil	Australia	MB118	TCTTATTCGTTGGAGACTTTATAACCCT	144.90557
BC15804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81895	WGS84	BC15804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15804_Cmae_Bot2011_COI	MB117	0	BC15804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15804	Sample	BC15804	targeted	Absent	Sample	Cmae_Bot2011_COI	MB117	COI	45	BC15804	1.00E-05	Nil	Australia	MB117	ATGAACAGTCTATCCTCCTTTAG	144.90584
BC15806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81908	WGS84	BC15806	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15806_Aamu_Bax2006_COI	MB111	0	BC15806_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15806	Sample	BC15806	targeted	Absent	Sample	Aamu_Bax2006_COI	MB111	COI	45	BC15806	1.00E-05	Nil	Australia	MB111	GCACAACCGGGATCTTTACTTC	144.90711
BC15806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81908	WGS84	BC15806	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.869	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15806_Sspa_Oph2007_COI	MB111	0	BC15806_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15806	Sample	BC15806	targeted	Present	Sample	Sspa_Oph2007_COI	MB111	COI	45	BC15806	1.00E-05	Nil	Australia	MB111	GAAGGCCTTCCGATGATC	144.90711
BC15806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81908	WGS84	BC15806	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15806_Upin_Bot2011_COI	MB111	0	BC15806_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15806	Sample	BC15806	targeted	Absent	Sample	Upin_Bot2011_COI	MB111	COI	45	BC15806	1.00E-05	Nil	Australia	MB111	CTTTAATTACAGCGTTTTTATTGTTGT	144.90711
BC15807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81996	WGS84	BC15807	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.533	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15807_Mgig_Bot2012_COI	MB109	0	BC15807_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15807	Sample	BC15807	targeted	Present	Sample	Mgig_Bot2012_COI	MB109	COI	45	BC15807	1.00E-05	Nil	Australia	MB109	TCTTATTCGTTGGAGACTTTATAACCCT	144.90884
BC15808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81918	WGS84	BC15808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15808_Cmae_Bot2011_COI	MB107	0	BC15808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15808	Sample	BC15808	targeted	Absent	Sample	Cmae_Bot2011_COI	MB107	COI	45	BC15808	1.00E-05	Nil	Australia	MB107	ATGAACAGTCTATCCTCCTTTAG	144.91054
BC15810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81528	WGS84	BC15810	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.344	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15810_Mgig_Bot2012_COI	MB103	0	BC15810_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15810	Sample	BC15810	targeted	Present	Sample	Mgig_Bot2012_COI	MB103	COI	45	BC15810	1.00E-05	Nil	Australia	MB103	TCTTATTCGTTGGAGACTTTATAACCCT	144.91289
BC15811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8126	WGS84	BC15811	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15811_Cmae_Bot2011_COI	MB101	0	BC15811_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15811	Sample	BC15811	targeted	Absent	Sample	Cmae_Bot2011_COI	MB101	COI	45	BC15811	1.00E-05	Nil	Australia	MB101	ATGAACAGTCTATCCTCCTTTAG	144.91319
BC15815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81832	WGS84	BC15815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15815_Cmae_Bot2011_COI	MA94	0	BC15815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15815	Sample	BC15815	targeted	Absent	Sample	Cmae_Bot2011_COI	MA94	COI	45	BC15815	1.00E-05	Nil	Australia	MA94	ATGAACAGTCTATCCTCCTTTAG	144.91822
BC15817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81763	WGS84	BC15817	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.027	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15817_Aamu_Bax2006_COI	MA92	0	BC15817_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15817	Sample	BC15817	targeted	Present	Sample	Aamu_Bax2006_COI	MA92	COI	45	BC15817	1.00E-05	Nil	Australia	MA92	GCACAACCGGGATCTTTACTTC	144.92
BC15817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81763	WGS84	BC15817	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.822	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15817_Sspa_Oph2007_COI	MA92	0	BC15817_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15817	Sample	BC15817	targeted	Present	Sample	Sspa_Oph2007_COI	MA92	COI	45	BC15817	1.00E-05	Nil	Australia	MA92	GAAGGCCTTCCGATGATC	144.92
BC15817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81763	WGS84	BC15817	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.511	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15817_Upin_Bot2011_COI	MA92	0	BC15817_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15817	Sample	BC15817	targeted	Present	Sample	Upin_Bot2011_COI	MA92	COI	45	BC15817	1.00E-05	Nil	Australia	MA92	CTTTAATTACAGCGTTTTTATTGTTGT	144.92
BC15818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85808	WGS84	BC15818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15818_Mgig_Bot2012_COI	MA9	0	BC15818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15818	Sample	BC15818	targeted	Absent	Sample	Mgig_Bot2012_COI	MA9	COI	45	BC15818	1.00E-05	Nil	Australia	MA9	TCTTATTCGTTGGAGACTTTATAACCCT	144.91
BC15819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81923	WGS84	BC15819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15819_Cmae_Bot2011_COI	MA89	0	BC15819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15819	Sample	BC15819	targeted	Absent	Sample	Cmae_Bot2011_COI	MA89	COI	45	BC15819	1.00E-05	Nil	Australia	MA89	ATGAACAGTCTATCCTCCTTTAG	144.91051
BC15820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85984	WGS84	BC15820	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.797	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15820_Aamu_Bax2006_COI	MA86	0	BC15820_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15820	Sample	BC15820	targeted	Present	Sample	Aamu_Bax2006_COI	MA86	COI	45	BC15820	1.00E-05	Nil	Australia	MA86	GCACAACCGGGATCTTTACTTC	144.91506
BC15820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85984	WGS84	BC15820	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.168	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15820_Sspa_Oph2007_COI	MA86	0	BC15820_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15820	Sample	BC15820	targeted	Present	Sample	Sspa_Oph2007_COI	MA86	COI	45	BC15820	1.00E-05	Nil	Australia	MA86	GAAGGCCTTCCGATGATC	144.91506
BC15820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85984	WGS84	BC15820	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.927	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15820_Upin_Bot2011_COI	MA86	0	BC15820_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15820	Sample	BC15820	targeted	Present	Sample	Upin_Bot2011_COI	MA86	COI	45	BC15820	1.00E-05	Nil	Australia	MA86	CTTTAATTACAGCGTTTTTATTGTTGT	144.91506
BC15821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85992	WGS84	BC15821	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15821_Mgig_Bot2012_COI	MA84	0	BC15821_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15821	Sample	BC15821	targeted	Absent	Sample	Mgig_Bot2012_COI	MA84	COI	45	BC15821	1.00E-05	Nil	Australia	MA84	TCTTATTCGTTGGAGACTTTATAACCCT	144.91472
BC15822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86324	WGS84	BC15822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15822_Cmae_Bot2011_COI	MA83	0	BC15822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15822	Sample	BC15822	targeted	Absent	Sample	Cmae_Bot2011_COI	MA83	COI	45	BC15822	1.00E-05	Nil	Australia	MA83	ATGAACAGTCTATCCTCCTTTAG	144.91424
BC15824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86328	WGS84	BC15824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.354	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15824_Aamu_Bax2006_COI	MA74	0	BC15824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15824	Sample	BC15824	targeted	Present	Sample	Aamu_Bax2006_COI	MA74	COI	45	BC15824	1.00E-05	Nil	Australia	MA74	GCACAACCGGGATCTTTACTTC	144.91845
BC15824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86328	WGS84	BC15824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.033	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15824_Sspa_Oph2007_COI	MA74	0	BC15824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15824	Sample	BC15824	targeted	Present	Sample	Sspa_Oph2007_COI	MA74	COI	45	BC15824	1.00E-05	Nil	Australia	MA74	GAAGGCCTTCCGATGATC	144.91845
BC15824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86328	WGS84	BC15824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.225	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15824_Upin_Bot2011_COI	MA74	0	BC15824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15824	Sample	BC15824	targeted	Present	Sample	Upin_Bot2011_COI	MA74	COI	45	BC15824	1.00E-05	Nil	Australia	MA74	CTTTAATTACAGCGTTTTTATTGTTGT	144.91845
BC15825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86447	WGS84	BC15825	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15825_Mgig_Bot2012_COI	MA73	0	BC15825_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15825	Sample	BC15825	targeted	Absent	Sample	Mgig_Bot2012_COI	MA73	COI	45	BC15825	1.00E-05	Nil	Australia	MA73	TCTTATTCGTTGGAGACTTTATAACCCT	144.91705
BC15832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8201	WGS84	BC15832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15832_Mgig_Bot2012_COI	MA56	0	BC15832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15832	Sample	BC15832	targeted	Absent	Sample	Mgig_Bot2012_COI	MA56	COI	45	BC15832	1.00E-05	Nil	Australia	MA56	TCTTATTCGTTGGAGACTTTATAACCCT	144.91851
BC15833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81978	WGS84	BC15833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15833_Cmae_Bot2011_COI	MA52	0	BC15833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15833	Sample	BC15833	targeted	Absent	Sample	Cmae_Bot2011_COI	MA52	COI	45	BC15833	1.00E-05	Nil	Australia	MA52	ATGAACAGTCTATCCTCCTTTAG	144.92565
BC15835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85732	WGS84	BC15835	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.686	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15835_Aamu_Bax2006_COI	MA44	0	BC15835_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15835	Sample	BC15835	targeted	Present	Sample	Aamu_Bax2006_COI	MA44	COI	45	BC15835	1.00E-05	Nil	Australia	MA44	GCACAACCGGGATCTTTACTTC	144.91012
BC15835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85732	WGS84	BC15835	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.682	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15835_Sspa_Oph2007_COI	MA44	0	BC15835_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15835	Sample	BC15835	targeted	Present	Sample	Sspa_Oph2007_COI	MA44	COI	45	BC15835	1.00E-05	Nil	Australia	MA44	GAAGGCCTTCCGATGATC	144.91012
BC15835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85732	WGS84	BC15835	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.739	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15835_Upin_Bot2011_COI	MA44	0	BC15835_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15835	Sample	BC15835	targeted	Present	Sample	Upin_Bot2011_COI	MA44	COI	45	BC15835	1.00E-05	Nil	Australia	MA44	CTTTAATTACAGCGTTTTTATTGTTGT	144.91012
BC15836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8645	WGS84	BC15836	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15836_Mgig_Bot2012_COI	MA42	0	BC15836_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15836	Sample	BC15836	targeted	Absent	Sample	Mgig_Bot2012_COI	MA42	COI	45	BC15836	1.00E-05	Nil	Australia	MA42	TCTTATTCGTTGGAGACTTTATAACCCT	144.9176
BC15837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81973	WGS84	BC15837	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15837_Cmae_Bot2011_COI	MA4	0	BC15837_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15837	Sample	BC15837	targeted	Absent	Sample	Cmae_Bot2011_COI	MA4	COI	45	BC15837	1.00E-05	Nil	Australia	MA4	ATGAACAGTCTATCCTCCTTTAG	144.92989
BC15839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84415	WGS84	BC15839	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.928	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15839_Aamu_Bax2006_COI	MA36	0	BC15839_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15839	Sample	BC15839	targeted	Present	Sample	Aamu_Bax2006_COI	MA36	COI	45	BC15839	1.00E-05	Nil	Australia	MA36	GCACAACCGGGATCTTTACTTC	144.90481
BC15839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84415	WGS84	BC15839	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.222	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15839_Sspa_Oph2007_COI	MA36	0	BC15839_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15839	Sample	BC15839	targeted	Present	Sample	Sspa_Oph2007_COI	MA36	COI	45	BC15839	1.00E-05	Nil	Australia	MA36	GAAGGCCTTCCGATGATC	144.90481
BC15839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84415	WGS84	BC15839	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15839_Upin_Bot2011_COI	MA36	0	BC15839_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15839	Sample	BC15839	targeted	Absent	Sample	Upin_Bot2011_COI	MA36	COI	45	BC15839	1.00E-05	Nil	Australia	MA36	CTTTAATTACAGCGTTTTTATTGTTGT	144.90481
BC15840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84748	WGS84	BC15840	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15840_Cmae_Bot2011_COI	MA34	0	BC15840_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15840	Sample	BC15840	targeted	Absent	Sample	Cmae_Bot2011_COI	MA34	COI	45	BC15840	1.00E-05	Nil	Australia	MA34	ATGAACAGTCTATCCTCCTTTAG	144.90998
BC15842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81938	WGS84	BC15842	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.408	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15842_Aamu_Bax2006_COI	MA3	0	BC15842_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15842	Sample	BC15842	targeted	Present	Sample	Aamu_Bax2006_COI	MA3	COI	45	BC15842	1.00E-05	Nil	Australia	MA3	GCACAACCGGGATCTTTACTTC	144.92775
BC15842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81938	WGS84	BC15842	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15842_Sspa_Oph2007_COI	MA3	0	BC15842_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15842	Sample	BC15842	targeted	Absent	Sample	Sspa_Oph2007_COI	MA3	COI	45	BC15842	1.00E-05	Nil	Australia	MA3	GAAGGCCTTCCGATGATC	144.92775
BC15842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81938	WGS84	BC15842	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15842_Upin_Bot2011_COI	MA3	0	BC15842_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15842	Sample	BC15842	targeted	Absent	Sample	Upin_Bot2011_COI	MA3	COI	45	BC15842	1.00E-05	Nil	Australia	MA3	CTTTAATTACAGCGTTTTTATTGTTGT	144.92775
BC15843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84537	WGS84	BC15843	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15843_Mgig_Bot2012_COI	MA29	0	BC15843_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15843	Sample	BC15843	targeted	Absent	Sample	Mgig_Bot2012_COI	MA29	COI	45	BC15843	1.00E-05	Nil	Australia	MA29	TCTTATTCGTTGGAGACTTTATAACCCT	144.9053
BC15844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84362	WGS84	BC15844	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15844_Cmae_Bot2011_COI	MA28	0	BC15844_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15844	Sample	BC15844	targeted	Absent	Sample	Cmae_Bot2011_COI	MA28	COI	45	BC15844	1.00E-05	Nil	Australia	MA28	ATGAACAGTCTATCCTCCTTTAG	144.90725
BC15846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84488	WGS84	BC15846	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.59	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15846_Aamu_Bax2006_COI	MA124	0	BC15846_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15846	Sample	BC15846	targeted	Present	Sample	Aamu_Bax2006_COI	MA124	COI	45	BC15846	1.00E-05	Nil	Australia	MA124	GCACAACCGGGATCTTTACTTC	144.93149
BC15846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84488	WGS84	BC15846	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.765	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15846_Sspa_Oph2007_COI	MA124	0	BC15846_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15846	Sample	BC15846	targeted	Present	Sample	Sspa_Oph2007_COI	MA124	COI	45	BC15846	1.00E-05	Nil	Australia	MA124	GAAGGCCTTCCGATGATC	144.93149
BC15846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84488	WGS84	BC15846	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.378	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15846_Upin_Bot2011_COI	MA124	0	BC15846_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15846	Sample	BC15846	targeted	Present	Sample	Upin_Bot2011_COI	MA124	COI	45	BC15846	1.00E-05	Nil	Australia	MA124	CTTTAATTACAGCGTTTTTATTGTTGT	144.93149
BC15847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84461	WGS84	BC15847	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15847_Mgig_Bot2012_COI	MA123	0	BC15847_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15847	Sample	BC15847	targeted	Absent	Sample	Mgig_Bot2012_COI	MA123	COI	45	BC15847	1.00E-05	Nil	Australia	MA123	TCTTATTCGTTGGAGACTTTATAACCCT	144.92611
BC15848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8601	WGS84	BC15848	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15848_Cmae_Bot2011_COI	MA122	0	BC15848_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15848	Sample	BC15848	targeted	Absent	Sample	Cmae_Bot2011_COI	MA122	COI	45	BC15848	1.00E-05	Nil	Australia	MA122	ATGAACAGTCTATCCTCCTTTAG	144.91211
BC15850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8436	WGS84	BC15850	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15850_Mgig_Bot2012_COI	MA114	0	BC15850_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15850	Sample	BC15850	targeted	Absent	Sample	Mgig_Bot2012_COI	MA114	COI	45	BC15850	1.00E-05	Nil	Australia	MA114	TCTTATTCGTTGGAGACTTTATAACCCT	144.93102
BC15851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82357	WGS84	BC15851	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15851_Cmae_Bot2011_COI	MA112	0	BC15851_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15851	Sample	BC15851	targeted	Absent	Sample	Cmae_Bot2011_COI	MA112	COI	45	BC15851	1.00E-05	Nil	Australia	MA112	ATGAACAGTCTATCCTCCTTTAG	144.90177
BC15853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82671	WGS84	BC15853	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.644	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15853_Aamu_Bax2006_COI	MA109	0	BC15853_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15853	Sample	BC15853	targeted	Present	Sample	Aamu_Bax2006_COI	MA109	COI	45	BC15853	1.00E-05	Nil	Australia	MA109	GCACAACCGGGATCTTTACTTC	144.89983
BC15853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82671	WGS84	BC15853	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.525	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15853_Sspa_Oph2007_COI	MA109	0	BC15853_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15853	Sample	BC15853	targeted	Present	Sample	Sspa_Oph2007_COI	MA109	COI	45	BC15853	1.00E-05	Nil	Australia	MA109	GAAGGCCTTCCGATGATC	144.89983
BC15853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82671	WGS84	BC15853	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.045	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15853_Upin_Bot2011_COI	MA109	0	BC15853_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15853	Sample	BC15853	targeted	Present	Sample	Upin_Bot2011_COI	MA109	COI	45	BC15853	1.00E-05	Nil	Australia	MA109	CTTTAATTACAGCGTTTTTATTGTTGT	144.89983
BC15854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8183	WGS84	BC15854	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15854_Mgig_Bot2012_COI	MA103	0	BC15854_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15854	Sample	BC15854	targeted	Absent	Sample	Mgig_Bot2012_COI	MA103	COI	45	BC15854	1.00E-05	Nil	Australia	MA103	TCTTATTCGTTGGAGACTTTATAACCCT	144.92655
BC15855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82107	WGS84	BC15855	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15855_Cmae_Bot2011_COI	MA102	0	BC15855_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15855	Sample	BC15855	targeted	Absent	Sample	Cmae_Bot2011_COI	MA102	COI	45	BC15855	1.00E-05	Nil	Australia	MA102	ATGAACAGTCTATCCTCCTTTAG	144.9053
BC15857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88505	WGS84	BC15857	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.331	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15857_Aamu_Bax2006_COI	HD18	0	BC15857_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15857	Sample	BC15857	targeted	Present	Sample	Aamu_Bax2006_COI	HD18	COI	45	BC15857	1.00E-05	Nil	Australia	HD18	GCACAACCGGGATCTTTACTTC	147.33662
BC15857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88505	WGS84	BC15857	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15857_Sspa_Oph2007_COI	HD18	0	BC15857_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15857	Sample	BC15857	targeted	Absent	Sample	Sspa_Oph2007_COI	HD18	COI	45	BC15857	1.00E-05	Nil	Australia	HD18	GAAGGCCTTCCGATGATC	147.33662
BC15857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88505	WGS84	BC15857	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15857_Upin_Bot2011_COI	HD18	0	BC15857_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15857	Sample	BC15857	targeted	Absent	Sample	Upin_Bot2011_COI	HD18	COI	45	BC15857	1.00E-05	Nil	Australia	HD18	CTTTAATTACAGCGTTTTTATTGTTGT	147.33662
BC15858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8768	WGS84	BC15858	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	15/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15858_Mgig_Bot2012_COI	HD12	0	BC15858_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15858	Sample	BC15858	targeted	Absent	Sample	Mgig_Bot2012_COI	HD12	COI	45	BC15858	1.00E-05	Nil	Australia	HD12	TCTTATTCGTTGGAGACTTTATAACCCT	147.34188
BC15859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.83088	WGS84	BC15859	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.249	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15859_Cmae_Bot2011_COI	HD1	0	BC15859_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15859	Sample	BC15859	targeted	Present	Sample	Cmae_Bot2011_COI	HD1	COI	45	BC15859	1.00E-05	Nil	Australia	HD1	ATGAACAGTCTATCCTCCTTTAG	147.32074
BC15860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8446	WGS84	BC15860	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15860_Aamu_Bax2006_COI	HC7	0	BC15860_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15860	Sample	BC15860	targeted	Absent	Sample	Aamu_Bax2006_COI	HC7	COI	45	BC15860	1.00E-05	Nil	Australia	HC7	GCACAACCGGGATCTTTACTTC	147.3313
BC15860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8446	WGS84	BC15860	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15860_Sspa_Oph2007_COI	HC7	0	BC15860_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15860	Sample	BC15860	targeted	Absent	Sample	Sspa_Oph2007_COI	HC7	COI	45	BC15860	1.00E-05	Nil	Australia	HC7	GAAGGCCTTCCGATGATC	147.3313
BC15860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8446	WGS84	BC15860	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15860_Upin_Bot2011_COI	HC7	0	BC15860_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15860	Sample	BC15860	targeted	Absent	Sample	Upin_Bot2011_COI	HC7	COI	45	BC15860	1.00E-05	Nil	Australia	HC7	CTTTAATTACAGCGTTTTTATTGTTGT	147.3313
BC15861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8434	WGS84	BC15861	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.146	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15861_Mgig_Bot2012_COI	HC6	0	BC15861_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15861	Sample	BC15861	targeted	Present	Sample	Mgig_Bot2012_COI	HC6	COI	45	BC15861	1.00E-05	Nil	Australia	HC6	TCTTATTCGTTGGAGACTTTATAACCCT	147.3301
BC15862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.873	WGS84	BC15862	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15862_Cmae_Bot2011_COI	HC24	0	BC15862_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15862	Sample	BC15862	targeted	Absent	Sample	Cmae_Bot2011_COI	HC24	COI	45	BC15862	1.00E-05	Nil	Australia	HC24	ATGAACAGTCTATCCTCCTTTAG	147.3366
BC15864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8293	WGS84	BC15864	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15864_Aamu_Bax2006_COI	HC2	0	BC15864_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15864	Sample	BC15864	targeted	Absent	Sample	Aamu_Bax2006_COI	HC2	COI	45	BC15864	1.00E-05	Nil	Australia	HC2	GCACAACCGGGATCTTTACTTC	147.3182
BC15864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8293	WGS84	BC15864	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15864_Sspa_Oph2007_COI	HC2	0	BC15864_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15864	Sample	BC15864	targeted	Absent	Sample	Sspa_Oph2007_COI	HC2	COI	45	BC15864	1.00E-05	Nil	Australia	HC2	GAAGGCCTTCCGATGATC	147.3182
BC15864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8293	WGS84	BC15864	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15864_Upin_Bot2011_COI	HC2	0	BC15864_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15864	Sample	BC15864	targeted	Absent	Sample	Upin_Bot2011_COI	HC2	COI	45	BC15864	1.00E-05	Nil	Australia	HC2	CTTTAATTACAGCGTTTTTATTGTTGT	147.3182
BC15865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8802	WGS84	BC15865	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.025	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15865_Mgig_Bot2012_COI	HC18	0	BC15865_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15865	Sample	BC15865	targeted	Present	Sample	Mgig_Bot2012_COI	HC18	COI	45	BC15865	1.00E-05	Nil	Australia	HC18	TCTTATTCGTTGGAGACTTTATAACCCT	147.3419
BC15866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8839	WGS84	BC15866	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15866_Cmae_Bot2011_COI	HC14	0	BC15866_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15866	Sample	BC15866	targeted	Absent	Sample	Cmae_Bot2011_COI	HC14	COI	45	BC15866	1.00E-05	Nil	Australia	HC14	ATGAACAGTCTATCCTCCTTTAG	147.3365
BC15868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88502	WGS84	BC15868	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.785	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15868_Aamu_Bax2006_COI	HB3	0	BC15868_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15868	Sample	BC15868	targeted	Present	Sample	Aamu_Bax2006_COI	HB3	COI	45	BC15868	1.00E-05	Nil	Australia	HB3	GCACAACCGGGATCTTTACTTC	147.33439
BC15868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88502	WGS84	BC15868	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15868_Sspa_Oph2007_COI	HB3	0	BC15868_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15868	Sample	BC15868	targeted	Absent	Sample	Sspa_Oph2007_COI	HB3	COI	45	BC15868	1.00E-05	Nil	Australia	HB3	GAAGGCCTTCCGATGATC	147.33439
BC15868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88502	WGS84	BC15868	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15868_Upin_Bot2011_COI	HB3	0	BC15868_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15868	Sample	BC15868	targeted	Absent	Sample	Upin_Bot2011_COI	HB3	COI	45	BC15868	1.00E-05	Nil	Australia	HB3	CTTTAATTACAGCGTTTTTATTGTTGT	147.33439
BC15869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.82939	WGS84	BC15869	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15869_Mgig_Bot2012_COI	HB23	0	BC15869_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15869	Sample	BC15869	targeted	Absent	Sample	Mgig_Bot2012_COI	HB23	COI	45	BC15869	1.00E-05	Nil	Australia	HB23	TCTTATTCGTTGGAGACTTTATAACCCT	147.31869
BC15871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84429	WGS84	BC15871	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.53	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15871_Aamu_Bax2006_COI	HB19	0	BC15871_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15871	Sample	BC15871	targeted	Present	Sample	Aamu_Bax2006_COI	HB19	COI	45	BC15871	1.00E-05	Nil	Australia	HB19	GCACAACCGGGATCTTTACTTC	147.33007
BC15871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84429	WGS84	BC15871	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15871_Sspa_Oph2007_COI	HB19	0	BC15871_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15871	Sample	BC15871	targeted	Absent	Sample	Sspa_Oph2007_COI	HB19	COI	45	BC15871	1.00E-05	Nil	Australia	HB19	GAAGGCCTTCCGATGATC	147.33007
BC15871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84429	WGS84	BC15871	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15871_Upin_Bot2011_COI	HB19	0	BC15871_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15871	Sample	BC15871	targeted	Absent	Sample	Upin_Bot2011_COI	HB19	COI	45	BC15871	1.00E-05	Nil	Australia	HB19	CTTTAATTACAGCGTTTTTATTGTTGT	147.33007
BC15872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84554	WGS84	BC15872	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15872_Mgig_Bot2012_COI	HB17	0	BC15872_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15872	Sample	BC15872	targeted	Absent	Sample	Mgig_Bot2012_COI	HB17	COI	45	BC15872	1.00E-05	Nil	Australia	HB17	TCTTATTCGTTGGAGACTTTATAACCCT	147.32933
BC15873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88523	WGS84	BC15873	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15873_Cmae_Bot2011_COI	HB12	0	BC15873_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15873	Sample	BC15873	targeted	Absent	Sample	Cmae_Bot2011_COI	HB12	COI	45	BC15873	1.00E-05	Nil	Australia	HB12	ATGAACAGTCTATCCTCCTTTAG	147.34082
BC15875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8191	WGS84	BC15875	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15875_Aamu_Bax2006_COI	MC69	0	BC15875_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15875	Sample	BC15875	targeted	Absent	Sample	Aamu_Bax2006_COI	MC69	COI	45	BC15875	1.00E-05	Nil	Australia	MC69	GCACAACCGGGATCTTTACTTC	144.92265
BC15875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8191	WGS84	BC15875	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.287	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15875_Sspa_Oph2007_COI	MC69	0	BC15875_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15875	Sample	BC15875	targeted	Present	Sample	Sspa_Oph2007_COI	MC69	COI	45	BC15875	1.00E-05	Nil	Australia	MC69	GAAGGCCTTCCGATGATC	144.92265
BC15875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8191	WGS84	BC15875	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15875_Upin_Bot2011_COI	MC69	0	BC15875_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15875	Sample	BC15875	targeted	Absent	Sample	Upin_Bot2011_COI	MC69	COI	45	BC15875	1.00E-05	Nil	Australia	MC69	CTTTAATTACAGCGTTTTTATTGTTGT	144.92265
BC15876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81962	WGS84	BC15876	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.947	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15876_Mgig_Bot2012_COI	MD99	0	BC15876_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15876	Sample	BC15876	targeted	Present	Sample	Mgig_Bot2012_COI	MD99	COI	45	BC15876	1.00E-05	Nil	Australia	MD99	TCTTATTCGTTGGAGACTTTATAACCCT	144.91304
BC15877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82039	WGS84	BC15877	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.497	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15877_Cmae_Bot2011_COI	MD97	0	BC15877_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15877	Sample	BC15877	targeted	Present	Sample	Cmae_Bot2011_COI	MD97	COI	45	BC15877	1.00E-05	Nil	Australia	MD97	ATGAACAGTCTATCCTCCTTTAG	144.91577
BC15879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81384	WGS84	BC15879	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.633	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15879_Aamu_Bax2006_COI	MD89	0	BC15879_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15879	Sample	BC15879	targeted	Present	Sample	Aamu_Bax2006_COI	MD89	COI	45	BC15879	1.00E-05	Nil	Australia	MD89	GCACAACCGGGATCTTTACTTC	144.91298
BC15879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81384	WGS84	BC15879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.281	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15879_Sspa_Oph2007_COI	MD89	0	BC15879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15879	Sample	BC15879	targeted	Present	Sample	Sspa_Oph2007_COI	MD89	COI	45	BC15879	1.00E-05	Nil	Australia	MD89	GAAGGCCTTCCGATGATC	144.91298
BC15879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81384	WGS84	BC15879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15879_Upin_Bot2011_COI	MD89	0	BC15879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15879	Sample	BC15879	targeted	Absent	Sample	Upin_Bot2011_COI	MD89	COI	45	BC15879	1.00E-05	Nil	Australia	MD89	CTTTAATTACAGCGTTTTTATTGTTGT	144.91298
BC15880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81641	WGS84	BC15880	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15880_Cmae_Bot2011_COI	MD87	0	BC15880_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15880	Sample	BC15880	targeted	Absent	Sample	Cmae_Bot2011_COI	MD87	COI	45	BC15880	1.00E-05	Nil	Australia	MD87	ATGAACAGTCTATCCTCCTTTAG	144.91328
BC15882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15882	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.07	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15882_Aamu_Bax2006_COI	MD81	0	BC15882_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15882	Sample	BC15882	targeted	Present	Sample	Aamu_Bax2006_COI	MD81	COI	45	BC15882	1.00E-05	Nil	Australia	MD81	GCACAACCGGGATCTTTACTTC	144.9188
BC15882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15882	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.716	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15882_Sspa_Oph2007_COI	MD81	0	BC15882_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15882	Sample	BC15882	targeted	Present	Sample	Sspa_Oph2007_COI	MD81	COI	45	BC15882	1.00E-05	Nil	Australia	MD81	GAAGGCCTTCCGATGATC	144.9188
BC15882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15882	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15882_Upin_Bot2011_COI	MD81	0	BC15882_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15882	Sample	BC15882	targeted	Absent	Sample	Upin_Bot2011_COI	MD81	COI	45	BC15882	1.00E-05	Nil	Australia	MD81	CTTTAATTACAGCGTTTTTATTGTTGT	144.9188
BC15883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8428	WGS84	BC15883	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15883_Mgig_Bot2012_COI	MD8	0	BC15883_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15883	Sample	BC15883	targeted	Absent	Sample	Mgig_Bot2012_COI	MD8	COI	45	BC15883	1.00E-05	Nil	Australia	MD8	TCTTATTCGTTGGAGACTTTATAACCCT	144.92954
BC15884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81769	WGS84	BC15884	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15884_Cmae_Bot2011_COI	MD78	0	BC15884_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15884	Sample	BC15884	targeted	Absent	Sample	Cmae_Bot2011_COI	MD78	COI	45	BC15884	1.00E-05	Nil	Australia	MD78	ATGAACAGTCTATCCTCCTTTAG	144.92064
BC15886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81919	WGS84	BC15886	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.362	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15886_Aamu_Bax2006_COI	MD72	0	BC15886_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15886	Sample	BC15886	targeted	Present	Sample	Aamu_Bax2006_COI	MD72	COI	45	BC15886	1.00E-05	Nil	Australia	MD72	GCACAACCGGGATCTTTACTTC	144.92412
BC15886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81919	WGS84	BC15886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.424	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15886_Sspa_Oph2007_COI	MD72	0	BC15886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15886	Sample	BC15886	targeted	Present	Sample	Sspa_Oph2007_COI	MD72	COI	45	BC15886	1.00E-05	Nil	Australia	MD72	GAAGGCCTTCCGATGATC	144.92412
BC15886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81919	WGS84	BC15886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15886_Upin_Bot2011_COI	MD72	0	BC15886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15886	Sample	BC15886	targeted	Absent	Sample	Upin_Bot2011_COI	MD72	COI	45	BC15886	1.00E-05	Nil	Australia	MD72	CTTTAATTACAGCGTTTTTATTGTTGT	144.92412
BC15887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81794	WGS84	BC15887	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15887_Mgig_Bot2012_COI	MD69	0	BC15887_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15887	Sample	BC15887	targeted	Absent	Sample	Mgig_Bot2012_COI	MD69	COI	45	BC15887	1.00E-05	Nil	Australia	MD69	TCTTATTCGTTGGAGACTTTATAACCCT	144.92451
BC15888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81643	WGS84	BC15888	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15888_Cmae_Bot2011_COI	MD67	0	BC15888_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15888	Sample	BC15888	targeted	Absent	Sample	Cmae_Bot2011_COI	MD67	COI	45	BC15888	1.00E-05	Nil	Australia	MD67	ATGAACAGTCTATCCTCCTTTAG	144.92564
BC15890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81966	WGS84	BC15890	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15890_Mgig_Bot2012_COI	MD64	0	BC15890_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15890	Sample	BC15890	targeted	Absent	Sample	Mgig_Bot2012_COI	MD64	COI	45	BC15890	1.00E-05	Nil	Australia	MD64	TCTTATTCGTTGGAGACTTTATAACCCT	144.92612
BC15891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81838	WGS84	BC15891	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15891_Cmae_Bot2011_COI	MD61	0	BC15891_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15891	Sample	BC15891	targeted	Absent	Sample	Cmae_Bot2011_COI	MD61	COI	45	BC15891	1.00E-05	Nil	Australia	MD61	ATGAACAGTCTATCCTCCTTTAG	144.92872
BC15893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15893	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.152	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15893_Aamu_Bax2006_COI	MD56	0	BC15893_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15893	Sample	BC15893	targeted	Present	Sample	Aamu_Bax2006_COI	MD56	COI	45	BC15893	1.00E-05	Nil	Australia	MD56	GCACAACCGGGATCTTTACTTC	144.93145
BC15893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15893	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.138	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15893_Sspa_Oph2007_COI	MD56	0	BC15893_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15893	Sample	BC15893	targeted	Present	Sample	Sspa_Oph2007_COI	MD56	COI	45	BC15893	1.00E-05	Nil	Australia	MD56	GAAGGCCTTCCGATGATC	144.93145
BC15893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15893	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15893_Upin_Bot2011_COI	MD56	0	BC15893_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15893	Sample	BC15893	targeted	Absent	Sample	Upin_Bot2011_COI	MD56	COI	45	BC15893	1.00E-05	Nil	Australia	MD56	CTTTAATTACAGCGTTTTTATTGTTGT	144.93145
BC15894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86038	WGS84	BC15894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15894_Mgig_Bot2012_COI	MD54	0	BC15894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15894	Sample	BC15894	targeted	Absent	Sample	Mgig_Bot2012_COI	MD54	COI	45	BC15894	1.00E-05	Nil	Australia	MD54	TCTTATTCGTTGGAGACTTTATAACCCT	144.90903
BC15895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85976	WGS84	BC15895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15895_Cmae_Bot2011_COI	MD52	0	BC15895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15895	Sample	BC15895	targeted	Absent	Sample	Cmae_Bot2011_COI	MD52	COI	45	BC15895	1.00E-05	Nil	Australia	MD52	ATGAACAGTCTATCCTCCTTTAG	144.91078
BC15897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86128	WGS84	BC15897	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.776	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15897_Aamu_Bax2006_COI	MD46	0	BC15897_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15897	Sample	BC15897	targeted	Present	Sample	Aamu_Bax2006_COI	MD46	COI	45	BC15897	1.00E-05	Nil	Australia	MD46	GCACAACCGGGATCTTTACTTC	144.91286
BC15897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86128	WGS84	BC15897	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.51	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15897_Sspa_Oph2007_COI	MD46	0	BC15897_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15897	Sample	BC15897	targeted	Present	Sample	Sspa_Oph2007_COI	MD46	COI	45	BC15897	1.00E-05	Nil	Australia	MD46	GAAGGCCTTCCGATGATC	144.91286
BC15897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86128	WGS84	BC15897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.621	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15897_Upin_Bot2011_COI	MD46	0	BC15897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15897	Sample	BC15897	targeted	Present	Sample	Upin_Bot2011_COI	MD46	COI	45	BC15897	1.00E-05	Nil	Australia	MD46	CTTTAATTACAGCGTTTTTATTGTTGT	144.91286
BC15898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86314	WGS84	BC15898	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.445	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15898_Mgig_Bot2012_COI	MD42	0	BC15898_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15898	Sample	BC15898	targeted	Present	Sample	Mgig_Bot2012_COI	MD42	COI	45	BC15898	1.00E-05	Nil	Australia	MD42	TCTTATTCGTTGGAGACTTTATAACCCT	144.91721
BC15899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86256	WGS84	BC15899	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15899_Cmae_Bot2011_COI	MD41	0	BC15899_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15899	Sample	BC15899	targeted	Absent	Sample	Cmae_Bot2011_COI	MD41	COI	45	BC15899	1.00E-05	Nil	Australia	MD41	ATGAACAGTCTATCCTCCTTTAG	144.91611
BC15901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86453	WGS84	BC15901	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.725	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15901_Aamu_Bax2006_COI	MD32	0	BC15901_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15901	Sample	BC15901	targeted	Present	Sample	Aamu_Bax2006_COI	MD32	COI	45	BC15901	1.00E-05	Nil	Australia	MD32	GCACAACCGGGATCTTTACTTC	144.91606
BC15901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86453	WGS84	BC15901	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.503	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15901_Sspa_Oph2007_COI	MD32	0	BC15901_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15901	Sample	BC15901	targeted	Present	Sample	Sspa_Oph2007_COI	MD32	COI	45	BC15901	1.00E-05	Nil	Australia	MD32	GAAGGCCTTCCGATGATC	144.91606
BC15901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86453	WGS84	BC15901	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.658	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15901_Upin_Bot2011_COI	MD32	0	BC15901_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15901	Sample	BC15901	targeted	Present	Sample	Upin_Bot2011_COI	MD32	COI	45	BC15901	1.00E-05	Nil	Australia	MD32	CTTTAATTACAGCGTTTTTATTGTTGT	144.91606
BC15902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86511	WGS84	BC15902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15902_Mgig_Bot2012_COI	MD31	0	BC15902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15902	Sample	BC15902	targeted	Absent	Sample	Mgig_Bot2012_COI	MD31	COI	45	BC15902	1.00E-05	Nil	Australia	MD31	TCTTATTCGTTGGAGACTTTATAACCCT	144.91823
BC15903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86574	WGS84	BC15903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15903_Cmae_Bot2011_COI	MD29	0	BC15903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15903	Sample	BC15903	targeted	Absent	Sample	Cmae_Bot2011_COI	MD29	COI	45	BC15903	1.00E-05	Nil	Australia	MD29	ATGAACAGTCTATCCTCCTTTAG	144.91648
BC15905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84831	WGS84	BC15905	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.178	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15905_Aamu_Bax2006_COI	MD24	0	BC15905_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15905	Sample	BC15905	targeted	Present	Sample	Aamu_Bax2006_COI	MD24	COI	45	BC15905	1.00E-05	Nil	Australia	MD24	GCACAACCGGGATCTTTACTTC	144.91069
BC15905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84831	WGS84	BC15905	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.794	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15905_Sspa_Oph2007_COI	MD24	0	BC15905_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15905	Sample	BC15905	targeted	Present	Sample	Sspa_Oph2007_COI	MD24	COI	45	BC15905	1.00E-05	Nil	Australia	MD24	GAAGGCCTTCCGATGATC	144.91069
BC15905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84831	WGS84	BC15905	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	43.634	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15905_Upin_Bot2011_COI	MD24	0	BC15905_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15905	Sample	BC15905	targeted	Present	Sample	Upin_Bot2011_COI	MD24	COI	45	BC15905	1.00E-05	Nil	Australia	MD24	CTTTAATTACAGCGTTTTTATTGTTGT	144.91069
BC15906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84846	WGS84	BC15906	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15906_Mgig_Bot2012_COI	MD22	0	BC15906_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15906	Sample	BC15906	targeted	Absent	Sample	Mgig_Bot2012_COI	MD22	COI	45	BC15906	1.00E-05	Nil	Australia	MD22	TCTTATTCGTTGGAGACTTTATAACCCT	144.90827
BC15907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84561	WGS84	BC15907	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15907_Cmae_Bot2011_COI	MD18	0	BC15907_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15907	Sample	BC15907	targeted	Absent	Sample	Cmae_Bot2011_COI	MD18	COI	45	BC15907	1.00E-05	Nil	Australia	MD18	ATGAACAGTCTATCCTCCTTTAG	144.90755
BC15909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84466	WGS84	BC15909	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.255	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15909_Aamu_Bax2006_COI	MD2	0	BC15909_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15909	Sample	BC15909	targeted	Present	Sample	Aamu_Bax2006_COI	MD2	COI	45	BC15909	1.00E-05	Nil	Australia	MD2	GCACAACCGGGATCTTTACTTC	144.93199
BC15909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84466	WGS84	BC15909	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.16	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15909_Sspa_Oph2007_COI	MD2	0	BC15909_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15909	Sample	BC15909	targeted	Present	Sample	Sspa_Oph2007_COI	MD2	COI	45	BC15909	1.00E-05	Nil	Australia	MD2	GAAGGCCTTCCGATGATC	144.93199
BC15909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84466	WGS84	BC15909	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15909_Upin_Bot2011_COI	MD2	0	BC15909_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15909	Sample	BC15909	targeted	Absent	Sample	Upin_Bot2011_COI	MD2	COI	45	BC15909	1.00E-05	Nil	Australia	MD2	CTTTAATTACAGCGTTTTTATTGTTGT	144.93199
BC15910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82758	WGS84	BC15910	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15910_Cmae_Bot2011_COI	MD126	0	BC15910_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15910	Sample	BC15910	targeted	Absent	Sample	Cmae_Bot2011_COI	MD126	COI	45	BC15910	1.00E-05	Nil	Australia	MD126	ATGAACAGTCTATCCTCCTTTAG	144.89942
BC15912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82515	WGS84	BC15912	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.658	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15912_Aamu_Bax2006_COI	MD121	0	BC15912_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15912	Sample	BC15912	targeted	Present	Sample	Aamu_Bax2006_COI	MD121	COI	45	BC15912	1.00E-05	Nil	Australia	MD121	GCACAACCGGGATCTTTACTTC	144.90263
BC15912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82515	WGS84	BC15912	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.244	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15912_Sspa_Oph2007_COI	MD121	0	BC15912_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15912	Sample	BC15912	targeted	Present	Sample	Sspa_Oph2007_COI	MD121	COI	45	BC15912	1.00E-05	Nil	Australia	MD121	GAAGGCCTTCCGATGATC	144.90263
BC15912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82515	WGS84	BC15912	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15912_Upin_Bot2011_COI	MD121	0	BC15912_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15912	Sample	BC15912	targeted	Absent	Sample	Upin_Bot2011_COI	MD121	COI	45	BC15912	1.00E-05	Nil	Australia	MD121	CTTTAATTACAGCGTTTTTATTGTTGT	144.90263
BC15913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82415	WGS84	BC15913	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15913_Mgig_Bot2012_COI	MD118	0	BC15913_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15913	Sample	BC15913	targeted	Absent	Sample	Mgig_Bot2012_COI	MD118	COI	45	BC15913	1.00E-05	Nil	Australia	MD118	TCTTATTCGTTGGAGACTTTATAACCCT	144.90236
BC15914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8234	WGS84	BC15914	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.122	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15914_Cmae_Bot2011_COI	MD116	0	BC15914_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15914	Sample	BC15914	targeted	Present	Sample	Cmae_Bot2011_COI	MD116	COI	45	BC15914	1.00E-05	Nil	Australia	MD116	ATGAACAGTCTATCCTCCTTTAG	144.906
BC15916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81945	WGS84	BC15916	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.337	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15916_Aamu_Bax2006_COI	MD112	0	BC15916_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15916	Sample	BC15916	targeted	Present	Sample	Aamu_Bax2006_COI	MD112	COI	45	BC15916	1.00E-05	Nil	Australia	MD112	GCACAACCGGGATCTTTACTTC	144.90621
BC15916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81945	WGS84	BC15916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.242	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15916_Sspa_Oph2007_COI	MD112	0	BC15916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15916	Sample	BC15916	targeted	Present	Sample	Sspa_Oph2007_COI	MD112	COI	45	BC15916	1.00E-05	Nil	Australia	MD112	GAAGGCCTTCCGATGATC	144.90621
BC15916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81945	WGS84	BC15916	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15916_Upin_Bot2011_COI	MD112	0	BC15916_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15916	Sample	BC15916	targeted	Absent	Sample	Upin_Bot2011_COI	MD112	COI	45	BC15916	1.00E-05	Nil	Australia	MD112	CTTTAATTACAGCGTTTTTATTGTTGT	144.90621
BC15917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82111	WGS84	BC15917	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15917_Mgig_Bot2012_COI	MD109	0	BC15917_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15917	Sample	BC15917	targeted	Absent	Sample	Mgig_Bot2012_COI	MD109	COI	45	BC15917	1.00E-05	Nil	Australia	MD109	TCTTATTCGTTGGAGACTTTATAACCCT	144.90803
BC15918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82047	WGS84	BC15918	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15918_Cmae_Bot2011_COI	MD103	0	BC15918_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15918	Sample	BC15918	targeted	Absent	Sample	Cmae_Bot2011_COI	MD103	COI	45	BC15918	1.00E-05	Nil	Australia	MD103	ATGAACAGTCTATCCTCCTTTAG	144.90914
BC15920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.84404	WGS84	BC15920	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15920_Mgig_Bot2012_COI	MD1	0	BC15920_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15920	Sample	BC15920	targeted	Absent	Sample	Mgig_Bot2012_COI	MD1	COI	45	BC15920	1.00E-05	Nil	Australia	MD1	TCTTATTCGTTGGAGACTTTATAACCCT	144.93298
BC15921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81914	WGS84	BC15921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15921_Cmae_Bot2011_COI	MC97	0	BC15921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15921	Sample	BC15921	targeted	Absent	Sample	Cmae_Bot2011_COI	MC97	COI	45	BC15921	1.00E-05	Nil	Australia	MC97	ATGAACAGTCTATCCTCCTTTAG	144.91473
BC15923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81339	WGS84	BC15923	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	42.698	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15923_Aamu_Bax2006_COI	MC92	0	BC15923_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15923	Sample	BC15923	targeted	Present	Sample	Aamu_Bax2006_COI	MC92	COI	45	BC15923	1.00E-05	Nil	Australia	MC92	GCACAACCGGGATCTTTACTTC	144.91286
BC15923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81339	WGS84	BC15923	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.196	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15923_Sspa_Oph2007_COI	MC92	0	BC15923_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15923	Sample	BC15923	targeted	Present	Sample	Sspa_Oph2007_COI	MC92	COI	45	BC15923	1.00E-05	Nil	Australia	MC92	GAAGGCCTTCCGATGATC	144.91286
BC15923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81339	WGS84	BC15923	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15923_Upin_Bot2011_COI	MC92	0	BC15923_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15923	Sample	BC15923	targeted	Absent	Sample	Upin_Bot2011_COI	MC92	COI	45	BC15923	1.00E-05	Nil	Australia	MC92	CTTTAATTACAGCGTTTTTATTGTTGT	144.91286
BC15924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81823	WGS84	BC15924	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.607	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15924_Mgig_Bot2012_COI	MC86	0	BC15924_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15924	Sample	BC15924	targeted	Present	Sample	Mgig_Bot2012_COI	MC86	COI	45	BC15924	1.00E-05	Nil	Australia	MC86	TCTTATTCGTTGGAGACTTTATAACCCT	144.91989
BC15925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.818	WGS84	BC15925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15925_Cmae_Bot2011_COI	MC84	0	BC15925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15925	Sample	BC15925	targeted	Absent	Sample	Cmae_Bot2011_COI	MC84	COI	45	BC15925	1.00E-05	Nil	Australia	MC84	ATGAACAGTCTATCCTCCTTTAG	144.92319
BC15927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84373	WGS84	BC15927	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.028	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15927_Aamu_Bax2006_COI	MC8	0	BC15927_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15927	Sample	BC15927	targeted	Present	Sample	Aamu_Bax2006_COI	MC8	COI	45	BC15927	1.00E-05	Nil	Australia	MC8	GCACAACCGGGATCTTTACTTC	144.92868
BC15927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84373	WGS84	BC15927	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.702	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15927_Sspa_Oph2007_COI	MC8	0	BC15927_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15927	Sample	BC15927	targeted	Present	Sample	Sspa_Oph2007_COI	MC8	COI	45	BC15927	1.00E-05	Nil	Australia	MC8	GAAGGCCTTCCGATGATC	144.92868
BC15927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84373	WGS84	BC15927	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	38.823	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15927_Upin_Bot2011_COI	MC8	0	BC15927_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15927	Sample	BC15927	targeted	Present	Sample	Upin_Bot2011_COI	MC8	COI	45	BC15927	1.00E-05	Nil	Australia	MC8	CTTTAATTACAGCGTTTTTATTGTTGT	144.92868
BC15928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81642	WGS84	BC15928	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.682	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15928_Mgig_Bot2012_COI	MC79	0	BC15928_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15928	Sample	BC15928	targeted	Present	Sample	Mgig_Bot2012_COI	MC79	COI	45	BC15928	1.00E-05	Nil	Australia	MC79	TCTTATTCGTTGGAGACTTTATAACCCT	144.92438
BC15929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81951	WGS84	BC15929	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15929_Cmae_Bot2011_COI	MC76	0	BC15929_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15929	Sample	BC15929	targeted	Absent	Sample	Cmae_Bot2011_COI	MC76	COI	45	BC15929	1.00E-05	Nil	Australia	MC76	ATGAACAGTCTATCCTCCTTTAG	144.92794
BC15930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81842	WGS84	BC15930	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15930_Aamu_Bax2006_COI	MC74	0	BC15930_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15930	Sample	BC15930	targeted	Absent	Sample	Aamu_Bax2006_COI	MC74	COI	45	BC15930	1.00E-05	Nil	Australia	MC74	GCACAACCGGGATCTTTACTTC	144.92982
BC15930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81842	WGS84	BC15930	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.079	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15930_Sspa_Oph2007_COI	MC74	0	BC15930_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15930	Sample	BC15930	targeted	Present	Sample	Sspa_Oph2007_COI	MC74	COI	45	BC15930	1.00E-05	Nil	Australia	MC74	GAAGGCCTTCCGATGATC	144.92982
BC15930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81842	WGS84	BC15930	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15930_Upin_Bot2011_COI	MC74	0	BC15930_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15930	Sample	BC15930	targeted	Absent	Sample	Upin_Bot2011_COI	MC74	COI	45	BC15930	1.00E-05	Nil	Australia	MC74	CTTTAATTACAGCGTTTTTATTGTTGT	144.92982
BC15931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81897	WGS84	BC15931	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.753	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15931_Mgig_Bot2012_COI	MC71	0	BC15931_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15931	Sample	BC15931	targeted	Present	Sample	Mgig_Bot2012_COI	MC71	COI	45	BC15931	1.00E-05	Nil	Australia	MC71	TCTTATTCGTTGGAGACTTTATAACCCT	144.92921
BC15932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81869	WGS84	BC15932	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15932_Cmae_Bot2011_COI	MC67	0	BC15932_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15932	Sample	BC15932	targeted	Absent	Sample	Cmae_Bot2011_COI	MC67	COI	45	BC15932	1.00E-05	Nil	Australia	MC67	ATGAACAGTCTATCCTCCTTTAG	144.91994
BC15934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82262	WGS84	BC15934	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15934_Aamu_Bax2006_COI	MC61	0	BC15934_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15934	Sample	BC15934	targeted	Absent	Sample	Aamu_Bax2006_COI	MC61	COI	45	BC15934	1.00E-05	Nil	Australia	MC61	GCACAACCGGGATCTTTACTTC	144.90604
BC15934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82262	WGS84	BC15934	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.562	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15934_Sspa_Oph2007_COI	MC61	0	BC15934_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15934	Sample	BC15934	targeted	Present	Sample	Sspa_Oph2007_COI	MC61	COI	45	BC15934	1.00E-05	Nil	Australia	MC61	GAAGGCCTTCCGATGATC	144.90604
BC15934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82262	WGS84	BC15934	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15934_Upin_Bot2011_COI	MC61	0	BC15934_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15934	Sample	BC15934	targeted	Absent	Sample	Upin_Bot2011_COI	MC61	COI	45	BC15934	1.00E-05	Nil	Australia	MC61	CTTTAATTACAGCGTTTTTATTGTTGT	144.90604
BC15935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84165	WGS84	BC15935	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.892	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15935_Mgig_Bot2012_COI	MC6	0	BC15935_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15935	Sample	BC15935	targeted	Present	Sample	Mgig_Bot2012_COI	MC6	COI	45	BC15935	1.00E-05	Nil	Australia	MC6	TCTTATTCGTTGGAGACTTTATAACCCT	144.92945
BC15936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82372	WGS84	BC15936	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15936_Cmae_Bot2011_COI	MC59	0	BC15936_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15936	Sample	BC15936	targeted	Absent	Sample	Cmae_Bot2011_COI	MC59	COI	45	BC15936	1.00E-05	Nil	Australia	MC59	ATGAACAGTCTATCCTCCTTTAG	144.90481
BC15938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82375	WGS84	BC15938	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.981	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15938_Aamu_Bax2006_COI	MC57	0	BC15938_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15938	Sample	BC15938	targeted	Present	Sample	Aamu_Bax2006_COI	MC57	COI	45	BC15938	1.00E-05	Nil	Australia	MC57	GCACAACCGGGATCTTTACTTC	144.90284
BC15938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82375	WGS84	BC15938	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.535	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15938_Sspa_Oph2007_COI	MC57	0	BC15938_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15938	Sample	BC15938	targeted	Present	Sample	Sspa_Oph2007_COI	MC57	COI	45	BC15938	1.00E-05	Nil	Australia	MC57	GAAGGCCTTCCGATGATC	144.90284
BC15938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82375	WGS84	BC15938	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15938_Upin_Bot2011_COI	MC57	0	BC15938_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15938	Sample	BC15938	targeted	Absent	Sample	Upin_Bot2011_COI	MC57	COI	45	BC15938	1.00E-05	Nil	Australia	MC57	CTTTAATTACAGCGTTTTTATTGTTGT	144.90284
BC15939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82484	WGS84	BC15939	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.608	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15939_Mgig_Bot2012_COI	MC56	0	BC15939_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15939	Sample	BC15939	targeted	Present	Sample	Mgig_Bot2012_COI	MC56	COI	45	BC15939	1.00E-05	Nil	Australia	MC56	TCTTATTCGTTGGAGACTTTATAACCCT	144.90287
BC15941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82723	WGS84	BC15941	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15941_Aamu_Bax2006_COI	MC49	0	BC15941_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15941	Sample	BC15941	targeted	Absent	Sample	Aamu_Bax2006_COI	MC49	COI	45	BC15941	1.00E-05	Nil	Australia	MC49	GCACAACCGGGATCTTTACTTC	144.9018
BC15941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82723	WGS84	BC15941	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.287	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15941_Sspa_Oph2007_COI	MC49	0	BC15941_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15941	Sample	BC15941	targeted	Present	Sample	Sspa_Oph2007_COI	MC49	COI	45	BC15941	1.00E-05	Nil	Australia	MC49	GAAGGCCTTCCGATGATC	144.9018
BC15941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82723	WGS84	BC15941	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15941_Upin_Bot2011_COI	MC49	0	BC15941_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15941	Sample	BC15941	targeted	Absent	Sample	Upin_Bot2011_COI	MC49	COI	45	BC15941	1.00E-05	Nil	Australia	MC49	CTTTAATTACAGCGTTTTTATTGTTGT	144.9018
BC15942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8663	WGS84	BC15942	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.093	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15942_Mgig_Bot2012_COI	MC31	0	BC15942_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15942	Sample	BC15942	targeted	Present	Sample	Mgig_Bot2012_COI	MC31	COI	45	BC15942	1.00E-05	Nil	Australia	MC31	TCTTATTCGTTGGAGACTTTATAACCCT	144.91593
BC15943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8442	WGS84	BC15943	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15943_Cmae_Bot2011_COI	MC3	0	BC15943_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15943	Sample	BC15943	targeted	Absent	Sample	Cmae_Bot2011_COI	MC3	COI	45	BC15943	1.00E-05	Nil	Australia	MC3	ATGAACAGTCTATCCTCCTTTAG	144.92999
BC15945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84115	WGS84	BC15945	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	40.093	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15945_Aamu_Bax2006_COI	MC27	0	BC15945_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15945	Sample	BC15945	targeted	Present	Sample	Aamu_Bax2006_COI	MC27	COI	45	BC15945	1.00E-05	Nil	Australia	MC27	GCACAACCGGGATCTTTACTTC	144.90495
BC15945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84115	WGS84	BC15945	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.874	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15945_Sspa_Oph2007_COI	MC27	0	BC15945_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15945	Sample	BC15945	targeted	Present	Sample	Sspa_Oph2007_COI	MC27	COI	45	BC15945	1.00E-05	Nil	Australia	MC27	GAAGGCCTTCCGATGATC	144.90495
BC15945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84115	WGS84	BC15945	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15945_Upin_Bot2011_COI	MC27	0	BC15945_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15945	Sample	BC15945	targeted	Absent	Sample	Upin_Bot2011_COI	MC27	COI	45	BC15945	1.00E-05	Nil	Australia	MC27	CTTTAATTACAGCGTTTTTATTGTTGT	144.90495
BC15946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84441	WGS84	BC15946	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.096	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15946_Mgig_Bot2012_COI	MC23	0	BC15946_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15946	Sample	BC15946	targeted	Present	Sample	Mgig_Bot2012_COI	MC23	COI	45	BC15946	1.00E-05	Nil	Australia	MC23	TCTTATTCGTTGGAGACTTTATAACCCT	144.90733
BC15947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84617	WGS84	BC15947	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15947_Cmae_Bot2011_COI	MC21	0	BC15947_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15947	Sample	BC15947	targeted	Absent	Sample	Cmae_Bot2011_COI	MC21	COI	45	BC15947	1.00E-05	Nil	Australia	MC21	ATGAACAGTCTATCCTCCTTTAG	144.90876
BC15949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84786	WGS84	BC15949	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15949_Aamu_Bax2006_COI	MC18	0	BC15949_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15949	Sample	BC15949	targeted	Absent	Sample	Aamu_Bax2006_COI	MC18	COI	45	BC15949	1.00E-05	Nil	Australia	MC18	GCACAACCGGGATCTTTACTTC	144.90858
BC15949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84786	WGS84	BC15949	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.528	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15949_Sspa_Oph2007_COI	MC18	0	BC15949_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15949	Sample	BC15949	targeted	Present	Sample	Sspa_Oph2007_COI	MC18	COI	45	BC15949	1.00E-05	Nil	Australia	MC18	GAAGGCCTTCCGATGATC	144.90858
BC15949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84786	WGS84	BC15949	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15949_Upin_Bot2011_COI	MC18	0	BC15949_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15949	Sample	BC15949	targeted	Absent	Sample	Upin_Bot2011_COI	MC18	COI	45	BC15949	1.00E-05	Nil	Australia	MC18	CTTTAATTACAGCGTTTTTATTGTTGT	144.90858
BC15950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8496	WGS84	BC15950	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15950_Cmae_Bot2011_COI	MC14	0	BC15950_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15950	Sample	BC15950	targeted	Absent	Sample	Cmae_Bot2011_COI	MC14	COI	45	BC15950	1.00E-05	Nil	Australia	MC14	ATGAACAGTCTATCCTCCTTTAG	144.90892
BC15952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82163	WGS84	BC15952	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15952_Aamu_Bax2006_COI	MC116	0	BC15952_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15952	Sample	BC15952	targeted	Absent	Sample	Aamu_Bax2006_COI	MC116	COI	45	BC15952	1.00E-05	Nil	Australia	MC116	GCACAACCGGGATCTTTACTTC	144.90604
BC15952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82163	WGS84	BC15952	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.394	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15952_Sspa_Oph2007_COI	MC116	0	BC15952_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15952	Sample	BC15952	targeted	Present	Sample	Sspa_Oph2007_COI	MC116	COI	45	BC15952	1.00E-05	Nil	Australia	MC116	GAAGGCCTTCCGATGATC	144.90604
BC15952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82163	WGS84	BC15952	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15952_Upin_Bot2011_COI	MC116	0	BC15952_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15952	Sample	BC15952	targeted	Absent	Sample	Upin_Bot2011_COI	MC116	COI	45	BC15952	1.00E-05	Nil	Australia	MC116	CTTTAATTACAGCGTTTTTATTGTTGT	144.90604
BC15953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85827	WGS84	BC15953	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.347	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15953_Mgig_Bot2012_COI	MC121	0	BC15953_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15953	Sample	BC15953	targeted	Present	Sample	Mgig_Bot2012_COI	MC121	COI	45	BC15953	1.00E-05	Nil	Australia	MC121	TCTTATTCGTTGGAGACTTTATAACCCT	144.90522
BC15954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82137	WGS84	BC15954	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15954_Cmae_Bot2011_COI	MC113	0	BC15954_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15954	Sample	BC15954	targeted	Absent	Sample	Cmae_Bot2011_COI	MC113	COI	45	BC15954	1.00E-05	Nil	Australia	MC113	ATGAACAGTCTATCCTCCTTTAG	144.91095
BC15956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81995	WGS84	BC15956	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15956_Aamu_Bax2006_COI	MC111	0	BC15956_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15956	Sample	BC15956	targeted	Absent	Sample	Aamu_Bax2006_COI	MC111	COI	45	BC15956	1.00E-05	Nil	Australia	MC111	GCACAACCGGGATCTTTACTTC	144.91018
BC15956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81995	WGS84	BC15956	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.084	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15956_Sspa_Oph2007_COI	MC111	0	BC15956_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15956	Sample	BC15956	targeted	Present	Sample	Sspa_Oph2007_COI	MC111	COI	45	BC15956	1.00E-05	Nil	Australia	MC111	GAAGGCCTTCCGATGATC	144.91018
BC15956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81995	WGS84	BC15956	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15956_Upin_Bot2011_COI	MC111	0	BC15956_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15956	Sample	BC15956	targeted	Absent	Sample	Upin_Bot2011_COI	MC111	COI	45	BC15956	1.00E-05	Nil	Australia	MC111	CTTTAATTACAGCGTTTTTATTGTTGT	144.91018
BC15957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84766	WGS84	BC15957	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.771	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15957_Mgig_Bot2012_COI	MC11	0	BC15957_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15957	Sample	BC15957	targeted	Present	Sample	Mgig_Bot2012_COI	MC11	COI	45	BC15957	1.00E-05	Nil	Australia	MC11	TCTTATTCGTTGGAGACTTTATAACCCT	144.93078
BC15958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81946	WGS84	BC15958	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15958_Cmae_Bot2011_COI	MC109	0	BC15958_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15958	Sample	BC15958	targeted	Absent	Sample	Cmae_Bot2011_COI	MC109	COI	45	BC15958	1.00E-05	Nil	Australia	MC109	ATGAACAGTCTATCCTCCTTTAG	144.91081
BC15960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81039	WGS84	BC15960	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.884	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15960_Mgig_Bot2012_COI	MB99	0	BC15960_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15960	Sample	BC15960	targeted	Present	Sample	Mgig_Bot2012_COI	MB99	COI	45	BC15960	1.00E-05	Nil	Australia	MB99	TCTTATTCGTTGGAGACTTTATAACCCT	144.91343
BC15961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.80951	WGS84	BC15961	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15961_Cmae_Bot2011_COI	MB97	0	BC15961_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15961	Sample	BC15961	targeted	Absent	Sample	Cmae_Bot2011_COI	MB97	COI	45	BC15961	1.00E-05	Nil	Australia	MB97	ATGAACAGTCTATCCTCCTTTAG	144.91335
BC15963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81548	WGS84	BC15963	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15963_Aamu_Bax2006_COI	MB91	0	BC15963_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15963	Sample	BC15963	targeted	Absent	Sample	Aamu_Bax2006_COI	MB91	COI	45	BC15963	1.00E-05	Nil	Australia	MB91	GCACAACCGGGATCTTTACTTC	144.9145
BC15963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81548	WGS84	BC15963	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.031	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15963_Sspa_Oph2007_COI	MB91	0	BC15963_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15963	Sample	BC15963	targeted	Present	Sample	Sspa_Oph2007_COI	MB91	COI	45	BC15963	1.00E-05	Nil	Australia	MB91	GAAGGCCTTCCGATGATC	144.9145
BC15963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81548	WGS84	BC15963	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15963_Upin_Bot2011_COI	MB91	0	BC15963_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15963	Sample	BC15963	targeted	Absent	Sample	Upin_Bot2011_COI	MB91	COI	45	BC15963	1.00E-05	Nil	Australia	MB91	CTTTAATTACAGCGTTTTTATTGTTGT	144.9145
BC15964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81861	WGS84	BC15964	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.242	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15964_Mgig_Bot2012_COI	MB88	0	BC15964_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15964	Sample	BC15964	targeted	Present	Sample	Mgig_Bot2012_COI	MB88	COI	45	BC15964	1.00E-05	Nil	Australia	MB88	TCTTATTCGTTGGAGACTTTATAACCCT	144.9163
BC15965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81788	WGS84	BC15965	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15965_Cmae_Bot2011_COI	MB86	0	BC15965_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15965	Sample	BC15965	targeted	Absent	Sample	Cmae_Bot2011_COI	MB86	COI	45	BC15965	1.00E-05	Nil	Australia	MB86	ATGAACAGTCTATCCTCCTTTAG	144.91938
BC15967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81564	WGS84	BC15967	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15967_Aamu_Bax2006_COI	MB82	0	BC15967_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15967	Sample	BC15967	targeted	Absent	Sample	Aamu_Bax2006_COI	MB82	COI	45	BC15967	1.00E-05	Nil	Australia	MB82	GCACAACCGGGATCTTTACTTC	144.92523
BC15967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81564	WGS84	BC15967	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15967_Sspa_Oph2007_COI	MB82	0	BC15967_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15967	Sample	BC15967	targeted	Absent	Sample	Sspa_Oph2007_COI	MB82	COI	45	BC15967	1.00E-05	Nil	Australia	MB82	GAAGGCCTTCCGATGATC	144.92523
BC15967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81564	WGS84	BC15967	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15967_Upin_Bot2011_COI	MB82	0	BC15967_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15967	Sample	BC15967	targeted	Absent	Sample	Upin_Bot2011_COI	MB82	COI	45	BC15967	1.00E-05	Nil	Australia	MB82	CTTTAATTACAGCGTTTTTATTGTTGT	144.92523
BC15968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84347	WGS84	BC15968	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15968_Mgig_Bot2012_COI	MB8	0	BC15968_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15968	Sample	BC15968	targeted	Absent	Sample	Mgig_Bot2012_COI	MB8	COI	45	BC15968	1.00E-05	Nil	Australia	MB8	TCTTATTCGTTGGAGACTTTATAACCCT	144.90609
BC15969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81593	WGS84	BC15969	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15969_Cmae_Bot2011_COI	MB79	0	BC15969_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15969	Sample	BC15969	targeted	Absent	Sample	Cmae_Bot2011_COI	MB79	COI	45	BC15969	1.00E-05	Nil	Australia	MB79	ATGAACAGTCTATCCTCCTTTAG	144.92751
BC15970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81794	WGS84	BC15970	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15970_Aamu_Bax2006_COI	MB74	0	BC15970_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15970	Sample	BC15970	targeted	Absent	Sample	Aamu_Bax2006_COI	MB74	COI	45	BC15970	1.00E-05	Nil	Australia	MB74	GCACAACCGGGATCTTTACTTC	144.93041
BC15970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81794	WGS84	BC15970	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.688	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15970_Sspa_Oph2007_COI	MB74	0	BC15970_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15970	Sample	BC15970	targeted	Present	Sample	Sspa_Oph2007_COI	MB74	COI	45	BC15970	1.00E-05	Nil	Australia	MB74	GAAGGCCTTCCGATGATC	144.93041
BC15970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81794	WGS84	BC15970	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15970_Upin_Bot2011_COI	MB74	0	BC15970_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15970	Sample	BC15970	targeted	Absent	Sample	Upin_Bot2011_COI	MB74	COI	45	BC15970	1.00E-05	Nil	Australia	MB74	CTTTAATTACAGCGTTTTTATTGTTGT	144.93041
BC15971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8205	WGS84	BC15971	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.466	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15971_Mgig_Bot2012_COI	MB68	0	BC15971_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15971	Sample	BC15971	targeted	Present	Sample	Mgig_Bot2012_COI	MB68	COI	45	BC15971	1.00E-05	Nil	Australia	MB68	TCTTATTCGTTGGAGACTTTATAACCCT	144.92968
BC15972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81981	WGS84	BC15972	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15972_Cmae_Bot2011_COI	MB64	0	BC15972_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15972	Sample	BC15972	targeted	Absent	Sample	Cmae_Bot2011_COI	MB64	COI	45	BC15972	1.00E-05	Nil	Australia	MB64	ATGAACAGTCTATCCTCCTTTAG	144.92026
BC15974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82323	WGS84	BC15974	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.405	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15974_Aamu_Bax2006_COI	MB59	0	BC15974_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15974	Sample	BC15974	targeted	Present	Sample	Aamu_Bax2006_COI	MB59	COI	45	BC15974	1.00E-05	Nil	Australia	MB59	GCACAACCGGGATCTTTACTTC	144.90667
BC15974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82323	WGS84	BC15974	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.673	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15974_Sspa_Oph2007_COI	MB59	0	BC15974_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15974	Sample	BC15974	targeted	Present	Sample	Sspa_Oph2007_COI	MB59	COI	45	BC15974	1.00E-05	Nil	Australia	MB59	GAAGGCCTTCCGATGATC	144.90667
BC15974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82323	WGS84	BC15974	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15974_Upin_Bot2011_COI	MB59	0	BC15974_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15974	Sample	BC15974	targeted	Absent	Sample	Upin_Bot2011_COI	MB59	COI	45	BC15974	1.00E-05	Nil	Australia	MB59	CTTTAATTACAGCGTTTTTATTGTTGT	144.90667
BC15975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82651	WGS84	BC15975	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.411	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15975_Mgig_Bot2012_COI	MB57	0	BC15975_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15975	Sample	BC15975	targeted	Present	Sample	Mgig_Bot2012_COI	MB57	COI	45	BC15975	1.00E-05	Nil	Australia	MB57	TCTTATTCGTTGGAGACTTTATAACCCT	144.90242
BC15976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85669	WGS84	BC15976	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15976_Cmae_Bot2011_COI	MB53	0	BC15976_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15976	Sample	BC15976	targeted	Absent	Sample	Cmae_Bot2011_COI	MB53	COI	45	BC15976	1.00E-05	Nil	Australia	MB53	ATGAACAGTCTATCCTCCTTTAG	144.90554
BC15978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86119	WGS84	BC15978	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.058	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15978_Aamu_Bax2006_COI	MB48	0	BC15978_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15978	Sample	BC15978	targeted	Present	Sample	Aamu_Bax2006_COI	MB48	COI	45	BC15978	1.00E-05	Nil	Australia	MB48	GCACAACCGGGATCTTTACTTC	144.90855
BC15978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86119	WGS84	BC15978	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.811	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15978_Sspa_Oph2007_COI	MB48	0	BC15978_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15978	Sample	BC15978	targeted	Present	Sample	Sspa_Oph2007_COI	MB48	COI	45	BC15978	1.00E-05	Nil	Australia	MB48	GAAGGCCTTCCGATGATC	144.90855
BC15978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86119	WGS84	BC15978	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.472	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15978_Upin_Bot2011_COI	MB48	0	BC15978_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15978	Sample	BC15978	targeted	Present	Sample	Upin_Bot2011_COI	MB48	COI	45	BC15978	1.00E-05	Nil	Australia	MB48	CTTTAATTACAGCGTTTTTATTGTTGT	144.90855
BC15979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86151	WGS84	BC15979	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15979_Mgig_Bot2012_COI	MB44	0	BC15979_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15979	Sample	BC15979	targeted	Absent	Sample	Mgig_Bot2012_COI	MB44	COI	45	BC15979	1.00E-05	Nil	Australia	MB44	TCTTATTCGTTGGAGACTTTATAACCCT	144.90993
BC15981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8626	WGS84	BC15981	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.682	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15981_Aamu_Bax2006_COI	MB41	0	BC15981_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15981	Sample	BC15981	targeted	Present	Sample	Aamu_Bax2006_COI	MB41	COI	45	BC15981	1.00E-05	Nil	Australia	MB41	GCACAACCGGGATCTTTACTTC	144.91343
BC15981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8626	WGS84	BC15981	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.895	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15981_Sspa_Oph2007_COI	MB41	0	BC15981_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15981	Sample	BC15981	targeted	Present	Sample	Sspa_Oph2007_COI	MB41	COI	45	BC15981	1.00E-05	Nil	Australia	MB41	GAAGGCCTTCCGATGATC	144.91343
BC15981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8626	WGS84	BC15981	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.535	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15981_Upin_Bot2011_COI	MB41	0	BC15981_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15981	Sample	BC15981	targeted	Present	Sample	Upin_Bot2011_COI	MB41	COI	45	BC15981	1.00E-05	Nil	Australia	MB41	CTTTAATTACAGCGTTTTTATTGTTGT	144.91343
BC15982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84074	WGS84	BC15982	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15982_Mgig_Bot2012_COI	MB4	0	BC15982_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15982	Sample	BC15982	targeted	Absent	Sample	Mgig_Bot2012_COI	MB4	COI	45	BC15982	1.00E-05	Nil	Australia	MB4	TCTTATTCGTTGGAGACTTTATAACCCT	144.90425
BC15983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8632	WGS84	BC15983	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15983_Cmae_Bot2011_COI	MB37	0	BC15983_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15983	Sample	BC15983	targeted	Absent	Sample	Cmae_Bot2011_COI	MB37	COI	45	BC15983	1.00E-05	Nil	Australia	MB37	ATGAACAGTCTATCCTCCTTTAG	144.91774
BC15985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86493	WGS84	BC15985	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.901	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15985_Aamu_Bax2006_COI	MB34	0	BC15985_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15985	Sample	BC15985	targeted	Present	Sample	Aamu_Bax2006_COI	MB34	COI	45	BC15985	1.00E-05	Nil	Australia	MB34	GCACAACCGGGATCTTTACTTC	144.91762
BC15985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86493	WGS84	BC15985	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.647	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15985_Sspa_Oph2007_COI	MB34	0	BC15985_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15985	Sample	BC15985	targeted	Present	Sample	Sspa_Oph2007_COI	MB34	COI	45	BC15985	1.00E-05	Nil	Australia	MB34	GAAGGCCTTCCGATGATC	144.91762
BC15985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86493	WGS84	BC15985	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.195	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15985_Upin_Bot2011_COI	MB34	0	BC15985_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15985	Sample	BC15985	targeted	Present	Sample	Upin_Bot2011_COI	MB34	COI	45	BC15985	1.00E-05	Nil	Australia	MB34	CTTTAATTACAGCGTTTTTATTGTTGT	144.91762
BC15986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86421	WGS84	BC15986	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15986_Mgig_Bot2012_COI	MB31	0	BC15986_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15986	Sample	BC15986	targeted	Absent	Sample	Mgig_Bot2012_COI	MB31	COI	45	BC15986	1.00E-05	Nil	Australia	MB31	TCTTATTCGTTGGAGACTTTATAACCCT	144.91498
BC15987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84065	WGS84	BC15987	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15987_Cmae_Bot2011_COI	MB3	0	BC15987_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15987	Sample	BC15987	targeted	Absent	Sample	Cmae_Bot2011_COI	MB3	COI	45	BC15987	1.00E-05	Nil	Australia	MB3	ATGAACAGTCTATCCTCCTTTAG	144.90472
BC25830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.81013	WGS84	BC25830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25830_Aamu_Bax2006_COI	T18-04	1	BC25830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25830	Sample	BC25830	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-04	COI	45	BC25830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-04	GCACAACCGGGATCTTTACTTC	147.50249
BC25830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.81013	WGS84	BC25830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25830_Pcan_Bot2011_IGS	T18-04	1	BC25830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25830	Sample	BC25830	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-04	IGS	45	BC25830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-04	CGTAATCCTCAGTACTGGCTA	147.50249
BC25830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.81013	WGS84	BC25830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25830_Sspa_Oph2007_COI	T18-04	1	BC25830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25830	Sample	BC25830	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-04	COI	45	BC25830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-04	GAAGGCCTTCCGATGATC	147.50249
BC25830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.81013	WGS84	BC25830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25830_Upin_Bot2011_COI	T18-04	1	BC25830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25830	Sample	BC25830	targeted	Absent	Sample	Upin_Bot2011_COI	T18-04	COI	45	BC25830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.50249
BC25831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80376	WGS84	BC25831	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25831_Cjap_Sim2018_COI	T18-05	1	BC25831_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25831	Sample	BC25831	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-05	COI	45	BC25831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52542
BC25831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80376	WGS84	BC25831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.446	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	28/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25831_Mgig_Bot2012_COI	T18-05	1	BC25831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25831	Sample	BC25831	targeted	Present	Sample	Mgig_Bot2012_COI	T18-05	COI	45	BC25831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.52542
BC25832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25832_Cmae_Bot2011_COI	T18-06	1	BC25832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25832	Sample	BC25832	targeted	Absent	Sample	Cmae_Bot2011_COI	T18-06	COI	45	BC25832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-06	ATGAACAGTCTATCCTCCTTTAG	147.48547
BC25835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96402	WGS84	BC25835	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25835_Cjap_Sim2018_COI	T18-12	1	BC25835_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25835	Sample	BC25835	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-12	COI	45	BC25835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53822
BC25835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96402	WGS84	BC25835	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.307	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	14/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25835_Mgig_Bot2012_COI	T18-12	1	BC25835_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25835	Sample	BC25835	targeted	Present	Sample	Mgig_Bot2012_COI	T18-12	COI	45	BC25835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.53822
BC25836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95973	WGS84	BC25836	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.399	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25836_Cmae_Bot2011_COI	T18-13	1	BC25836_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25836	Sample	BC25836	targeted	Present	Sample	Cmae_Bot2011_COI	T18-13	COI	45	BC25836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-13	ATGAACAGTCTATCCTCCTTTAG	147.52782
BC25838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25838	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25838_Aamu_Bax2006_COI	T18-15	1	BC25838_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25838	Sample	BC25838	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-15	COI	45	BC25838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-15	GCACAACCGGGATCTTTACTTC	147.52367
BC25838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25838	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25838_Pcan_Bot2011_IGS	T18-15	1	BC25838_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25838	Sample	BC25838	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-15	IGS	45	BC25838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-15	CGTAATCCTCAGTACTGGCTA	147.52367
BC25838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25838	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25838_Sspa_Oph2007_COI	T18-15	1	BC25838_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25838	Sample	BC25838	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-15	COI	45	BC25838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-15	GAAGGCCTTCCGATGATC	147.52367
BC25838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25838	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25838_Upin_Bot2011_COI	T18-15	1	BC25838_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25838	Sample	BC25838	targeted	Absent	Sample	Upin_Bot2011_COI	T18-15	COI	45	BC25838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.52367
BC25841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84676	WGS84	BC25841	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25841_Aamu_Bax2006_COI	S13-01	1	BC25841_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25841	Sample	BC25841	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-01	COI	45	BC25841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-01	GCACAACCGGGATCTTTACTTC	138.49723
BC25841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84676	WGS84	BC25841	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25841_Pcan_Bot2011_IGS	S13-01	1	BC25841_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25841	Sample	BC25841	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-01	IGS	45	BC25841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-01	CGTAATCCTCAGTACTGGCTA	138.49723
BC25841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84676	WGS84	BC25841	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25841_Sspa_Oph2007_COI	S13-01	1	BC25841_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25841	Sample	BC25841	targeted	Absent	Sample	Sspa_Oph2007_COI	S13-01	COI	45	BC25841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-01	GAAGGCCTTCCGATGATC	138.49723
BC25841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84676	WGS84	BC25841	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25841_Upin_Bot2011_COI	S13-01	1	BC25841_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25841	Sample	BC25841	targeted	Absent	Sample	Upin_Bot2011_COI	S13-01	COI	45	BC25841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.49723
BC25842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84602	WGS84	BC25842	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25842_Cjap_Sim2018_COI	S13-02	1	BC25842_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25842	Sample	BC25842	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-02	COI	45	BC25842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4969
BC25842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84602	WGS84	BC25842	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.641	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25842_Mgig_Bot2012_COI	S13-02	1	BC25842_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25842	Sample	BC25842	targeted	Present	Sample	Mgig_Bot2012_COI	S13-02	COI	45	BC25842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.4969
BC25843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84521	WGS84	BC25843	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25843_Cmae_Bot2011_COI	S13-03	1	BC25843_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25843	Sample	BC25843	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-03	COI	45	BC25843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-03	ATGAACAGTCTATCCTCCTTTAG	138.4969
BC25845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83671	WGS84	BC25845	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25845_Aamu_Bax2006_COI	S13-05	1	BC25845_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25845	Sample	BC25845	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-05	COI	45	BC25845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-05	GCACAACCGGGATCTTTACTTC	138.50903
BC25845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83671	WGS84	BC25845	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25845_Pcan_Bot2011_IGS	S13-05	1	BC25845_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25845	Sample	BC25845	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-05	IGS	45	BC25845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-05	CGTAATCCTCAGTACTGGCTA	138.50903
BC25845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83671	WGS84	BC25845	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.423	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25845_Sspa_Oph2007_COI	S13-05	1	BC25845_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25845	Sample	BC25845	targeted	Present	Sample	Sspa_Oph2007_COI	S13-05	COI	45	BC25845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-05	GAAGGCCTTCCGATGATC	138.50903
BC25845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83671	WGS84	BC25845	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25845_Upin_Bot2011_COI	S13-05	1	BC25845_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25845	Sample	BC25845	targeted	Absent	Sample	Upin_Bot2011_COI	S13-05	COI	45	BC25845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.50903
BC25846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83708	WGS84	BC25846	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25846_Cjap_Sim2018_COI	S13-06	1	BC25846_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25846	Sample	BC25846	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-06	COI	45	BC25846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51062
BC25846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83708	WGS84	BC25846	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.922	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25846_Mgig_Bot2012_COI	S13-06	1	BC25846_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25846	Sample	BC25846	targeted	Present	Sample	Mgig_Bot2012_COI	S13-06	COI	45	BC25846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.51062
BC25847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.7778	WGS84	BC25847	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25847_Cmae_Bot2011_COI	S13-07	1	BC25847_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25847	Sample	BC25847	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-07	COI	45	BC25847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-07	ATGAACAGTCTATCCTCCTTTAG	138.51836
BC25849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.78028	WGS84	BC25849	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25849_Aamu_Bax2006_COI	S13-09	1	BC25849_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25849	Sample	BC25849	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-09	COI	45	BC25849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-09	GCACAACCGGGATCTTTACTTC	138.51745
BC25849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.78028	WGS84	BC25849	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25849_Pcan_Bot2011_IGS	S13-09	1	BC25849_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25849	Sample	BC25849	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-09	IGS	45	BC25849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-09	CGTAATCCTCAGTACTGGCTA	138.51745
BC25849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.78028	WGS84	BC25849	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.562	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25849_Sspa_Oph2007_COI	S13-09	1	BC25849_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25849	Sample	BC25849	targeted	Present	Sample	Sspa_Oph2007_COI	S13-09	COI	45	BC25849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-09	GAAGGCCTTCCGATGATC	138.51745
BC25849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.78028	WGS84	BC25849	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25849_Upin_Bot2011_COI	S13-09	1	BC25849_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25849	Sample	BC25849	targeted	Absent	Sample	Upin_Bot2011_COI	S13-09	COI	45	BC25849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.51745
BC25850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80929	WGS84	BC25850	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25850_Cmae_Bot2011_COI	S13-10	1	BC25850_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25850	Sample	BC25850	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-10	COI	45	BC25850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-10	ATGAACAGTCTATCCTCCTTTAG	138.51861
BC25852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81041	WGS84	BC25852	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25852_Aamu_Bax2006_COI	S13-12	1	BC25852_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25852	Sample	BC25852	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-12	COI	45	BC25852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-12	GCACAACCGGGATCTTTACTTC	138.52065
BC25852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81041	WGS84	BC25852	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25852_Pcan_Bot2011_IGS	S13-12	1	BC25852_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25852	Sample	BC25852	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-12	IGS	45	BC25852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-12	CGTAATCCTCAGTACTGGCTA	138.52065
BC25852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81041	WGS84	BC25852	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.316	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25852_Sspa_Oph2007_COI	S13-12	1	BC25852_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25852	Sample	BC25852	targeted	Present	Sample	Sspa_Oph2007_COI	S13-12	COI	45	BC25852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-12	GAAGGCCTTCCGATGATC	138.52065
BC25852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81041	WGS84	BC25852	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25852_Upin_Bot2011_COI	S13-12	1	BC25852_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25852	Sample	BC25852	targeted	Absent	Sample	Upin_Bot2011_COI	S13-12	COI	45	BC25852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.52065
BC25853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83975	WGS84	BC25853	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25853_Cjap_Sim2018_COI	S14-01	1	BC25853_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25853	Sample	BC25853	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-01	COI	45	BC25853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50678
BC25853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83975	WGS84	BC25853	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.688	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25853_Mgig_Bot2012_COI	S14-01	1	BC25853_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25853	Sample	BC25853	targeted	Present	Sample	Mgig_Bot2012_COI	S14-01	COI	45	BC25853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.50678
BC25854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25854	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25854_Cmae_Bot2011_COI	S14-02	1	BC25854_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25854	Sample	BC25854	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-02	COI	45	BC25854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-02	ATGAACAGTCTATCCTCCTTTAG	138.50776
BC25856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83589	WGS84	BC25856	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25856_Aamu_Bax2006_COI	S14-04	1	BC25856_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25856	Sample	BC25856	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-04	COI	45	BC25856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-04	GCACAACCGGGATCTTTACTTC	138.50814
BC25856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83589	WGS84	BC25856	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25856_Pcan_Bot2011_IGS	S14-04	1	BC25856_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25856	Sample	BC25856	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-04	IGS	45	BC25856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-04	CGTAATCCTCAGTACTGGCTA	138.50814
BC25856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83589	WGS84	BC25856	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25856_Sspa_Oph2007_COI	S14-04	1	BC25856_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25856	Sample	BC25856	targeted	Absent	Sample	Sspa_Oph2007_COI	S14-04	COI	45	BC25856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-04	GAAGGCCTTCCGATGATC	138.50814
BC25856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83589	WGS84	BC25856	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25856_Upin_Bot2011_COI	S14-04	1	BC25856_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25856	Sample	BC25856	targeted	Absent	Sample	Upin_Bot2011_COI	S14-04	COI	45	BC25856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.50814
BC25857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83382	WGS84	BC25857	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25857_Cjap_Sim2018_COI	S14-05	1	BC25857_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25857	Sample	BC25857	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-05	COI	45	BC25857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50779
BC25857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83382	WGS84	BC25857	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.738	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25857_Mgig_Bot2012_COI	S14-05	1	BC25857_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25857	Sample	BC25857	targeted	Present	Sample	Mgig_Bot2012_COI	S14-05	COI	45	BC25857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.50779
BC25858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.809	WGS84	BC25858	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25858_Cmae_Bot2011_COI	S14-06	1	BC25858_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25858	Sample	BC25858	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-06	COI	45	BC25858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-06	ATGAACAGTCTATCCTCCTTTAG	138.51736
BC25860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80988	WGS84	BC25860	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25860_Cjap_Sim2018_COI	S14-08	1	BC25860_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25860	Sample	BC25860	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-08	COI	45	BC25860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51941
BC25860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80988	WGS84	BC25860	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.924	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25860_Mgig_Bot2012_COI	S14-08	1	BC25860_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25860	Sample	BC25860	targeted	Present	Sample	Mgig_Bot2012_COI	S14-08	COI	45	BC25860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51941
BC25861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81024	WGS84	BC25861	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25861_Cmae_Bot2011_COI	S14-09	1	BC25861_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25861	Sample	BC25861	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-09	COI	45	BC25861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-09	ATGAACAGTCTATCCTCCTTTAG	138.52043
BC25863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77237	WGS84	BC25863	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25863_Aamu_Bax2006_COI	S15-01	1	BC25863_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25863	Sample	BC25863	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-01	COI	45	BC25863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-01	GCACAACCGGGATCTTTACTTC	138.48606
BC25863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77237	WGS84	BC25863	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25863_Pcan_Bot2011_IGS	S15-01	1	BC25863_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25863	Sample	BC25863	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-01	IGS	45	BC25863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-01	CGTAATCCTCAGTACTGGCTA	138.48606
BC25863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77237	WGS84	BC25863	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25863_Sspa_Oph2007_COI	S15-01	1	BC25863_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25863	Sample	BC25863	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-01	COI	45	BC25863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-01	GAAGGCCTTCCGATGATC	138.48606
BC25863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77237	WGS84	BC25863	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25863_Upin_Bot2011_COI	S15-01	1	BC25863_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25863	Sample	BC25863	targeted	Absent	Sample	Upin_Bot2011_COI	S15-01	COI	45	BC25863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.48606
BC25864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77271	WGS84	BC25864	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25864_Cjap_Sim2018_COI	S15-02	1	BC25864_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25864	Sample	BC25864	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-02	COI	45	BC25864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48516
BC25864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77271	WGS84	BC25864	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.237	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25864_Mgig_Bot2012_COI	S15-02	1	BC25864_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25864	Sample	BC25864	targeted	Present	Sample	Mgig_Bot2012_COI	S15-02	COI	45	BC25864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.48516
BC25865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77213	WGS84	BC25865	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25865_Cmae_Bot2011_COI	S15-03	1	BC25865_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25865	Sample	BC25865	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-03	COI	45	BC25865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-03	ATGAACAGTCTATCCTCCTTTAG	138.48451
BC25867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77886	WGS84	BC25867	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25867_Aamu_Bax2006_COI	S15-05	1	BC25867_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25867	Sample	BC25867	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-05	COI	45	BC25867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-05	GCACAACCGGGATCTTTACTTC	138.51809
BC25867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77886	WGS84	BC25867	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25867_Pcan_Bot2011_IGS	S15-05	1	BC25867_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25867	Sample	BC25867	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-05	IGS	45	BC25867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-05	CGTAATCCTCAGTACTGGCTA	138.51809
BC25867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77886	WGS84	BC25867	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25867_Sspa_Oph2007_COI	S15-05	1	BC25867_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25867	Sample	BC25867	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-05	COI	45	BC25867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-05	GAAGGCCTTCCGATGATC	138.51809
BC25867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77886	WGS84	BC25867	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25867_Upin_Bot2011_COI	S15-05	1	BC25867_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25867	Sample	BC25867	targeted	Absent	Sample	Upin_Bot2011_COI	S15-05	COI	45	BC25867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.51809
BC25868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77988	WGS84	BC25868	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25868_Cjap_Sim2018_COI	S15-06	1	BC25868_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25868	Sample	BC25868	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-06	COI	45	BC25868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51786
BC25868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77988	WGS84	BC25868	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.205	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25868_Mgig_Bot2012_COI	S15-06	1	BC25868_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25868	Sample	BC25868	targeted	Present	Sample	Mgig_Bot2012_COI	S15-06	COI	45	BC25868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.51786
BC25869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80875	WGS84	BC25869	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25869_Cmae_Bot2011_COI	S15-07	1	BC25869_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25869	Sample	BC25869	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-07	COI	45	BC25869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-07	ATGAACAGTCTATCCTCCTTTAG	138.51685
BC25870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80904	WGS84	BC25870	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25870_Aamu_Bax2006_COI	S15-08	1	BC25870_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25870	Sample	BC25870	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-08	COI	45	BC25870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-08	GCACAACCGGGATCTTTACTTC	138.51774
BC25870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80904	WGS84	BC25870	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25870_Pcan_Bot2011_IGS	S15-08	1	BC25870_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25870	Sample	BC25870	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-08	IGS	45	BC25870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-08	CGTAATCCTCAGTACTGGCTA	138.51774
BC25870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80904	WGS84	BC25870	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25870_Sspa_Oph2007_COI	S15-08	1	BC25870_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25870	Sample	BC25870	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-08	COI	45	BC25870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-08	GAAGGCCTTCCGATGATC	138.51774
BC25870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80904	WGS84	BC25870	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25870_Upin_Bot2011_COI	S15-08	1	BC25870_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25870	Sample	BC25870	targeted	Absent	Sample	Upin_Bot2011_COI	S15-08	COI	45	BC25870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51774
BC25871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80946	WGS84	BC25871	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25871_Cjap_Sim2018_COI	S15-09	1	BC25871_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25871	Sample	BC25871	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-09	COI	45	BC25871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51863
BC25871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80946	WGS84	BC25871	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.977	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25871_Mgig_Bot2012_COI	S15-09	1	BC25871_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25871	Sample	BC25871	targeted	Present	Sample	Mgig_Bot2012_COI	S15-09	COI	45	BC25871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51863
BC25872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83621	WGS84	BC25872	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25872_Cmae_Bot2011_COI	S15-10	1	BC25872_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25872	Sample	BC25872	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-10	COI	45	BC25872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-10	ATGAACAGTCTATCCTCCTTTAG	138.50868
BC25874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83695	WGS84	BC25874	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25874_Aamu_Bax2006_COI	S15-12	1	BC25874_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25874	Sample	BC25874	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-12	COI	45	BC25874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-12	GCACAACCGGGATCTTTACTTC	138.50914
BC25874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83695	WGS84	BC25874	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25874_Pcan_Bot2011_IGS	S15-12	1	BC25874_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25874	Sample	BC25874	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-12	IGS	45	BC25874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-12	CGTAATCCTCAGTACTGGCTA	138.50914
BC25874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83695	WGS84	BC25874	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.329	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25874_Sspa_Oph2007_COI	S15-12	1	BC25874_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25874	Sample	BC25874	targeted	Present	Sample	Sspa_Oph2007_COI	S15-12	COI	45	BC25874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-12	GAAGGCCTTCCGATGATC	138.50914
BC25874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83695	WGS84	BC25874	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25874_Upin_Bot2011_COI	S15-12	1	BC25874_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25874	Sample	BC25874	targeted	Absent	Sample	Upin_Bot2011_COI	S15-12	COI	45	BC25874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.50914
BC25875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84716	WGS84	BC25875	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25875_Cjap_Sim2018_COI	S15-13	1	BC25875_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25875	Sample	BC25875	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-13	COI	45	BC25875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49769
BC25875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84716	WGS84	BC25875	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.2	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25875_Mgig_Bot2012_COI	S15-13	1	BC25875_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25875	Sample	BC25875	targeted	Present	Sample	Mgig_Bot2012_COI	S15-13	COI	45	BC25875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.49769
BC25876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84566	WGS84	BC25876	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25876_Cmae_Bot2011_COI	S15-14	1	BC25876_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25876	Sample	BC25876	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-14	COI	45	BC25876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-14	ATGAACAGTCTATCCTCCTTTAG	138.4971
BC25878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96022	WGS84	BC25878	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25878_Aamu_Bax2006_COI	T19-01	1	BC25878_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25878	Sample	BC25878	targeted	Absent	Sample	Aamu_Bax2006_COI	T19-01	COI	45	BC25878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-01	GCACAACCGGGATCTTTACTTC	147.52332
BC25878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96022	WGS84	BC25878	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25878_Pcan_Bot2011_IGS	T19-01	1	BC25878_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25878	Sample	BC25878	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-01	IGS	45	BC25878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-01	CGTAATCCTCAGTACTGGCTA	147.52332
BC25878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96022	WGS84	BC25878	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25878_Sspa_Oph2007_COI	T19-01	1	BC25878_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25878	Sample	BC25878	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-01	COI	45	BC25878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-01	GAAGGCCTTCCGATGATC	147.52332
BC25878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96022	WGS84	BC25878	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25878_Upin_Bot2011_COI	T19-01	1	BC25878_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25878	Sample	BC25878	targeted	Absent	Sample	Upin_Bot2011_COI	T19-01	COI	45	BC25878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.52332
BC25879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96277	WGS84	BC25879	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25879_Cjap_Sim2018_COI	T19-02	1	BC25879_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25879	Sample	BC25879	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-02	COI	45	BC25879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53956
BC25879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96277	WGS84	BC25879	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.147	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	13/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25879_Mgig_Bot2012_COI	T19-02	1	BC25879_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25879	Sample	BC25879	targeted	Present	Sample	Mgig_Bot2012_COI	T19-02	COI	45	BC25879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.53956
BC25881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96467	WGS84	BC25881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.067	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25881_Aamu_Bax2006_COI	T19-04	1	BC25881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25881	Sample	BC25881	targeted	Present	Sample	Aamu_Bax2006_COI	T19-04	COI	45	BC25881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-04	GCACAACCGGGATCTTTACTTC	147.51968
BC25881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96467	WGS84	BC25881	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25881_Pcan_Bot2011_IGS	T19-04	1	BC25881_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25881	Sample	BC25881	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-04	IGS	45	BC25881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-04	CGTAATCCTCAGTACTGGCTA	147.51968
BC25881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96467	WGS84	BC25881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25881_Sspa_Oph2007_COI	T19-04	1	BC25881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25881	Sample	BC25881	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-04	COI	45	BC25881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-04	GAAGGCCTTCCGATGATC	147.51968
BC25881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96467	WGS84	BC25881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25881_Upin_Bot2011_COI	T19-04	1	BC25881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25881	Sample	BC25881	targeted	Absent	Sample	Upin_Bot2011_COI	T19-04	COI	45	BC25881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.51968
BC25882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95965	WGS84	BC25882	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25882_Cjap_Sim2018_COI	T19-05	1	BC25882_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25882	Sample	BC25882	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-05	COI	45	BC25882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52786
BC25882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95965	WGS84	BC25882	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.981	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	13/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25882_Mgig_Bot2012_COI	T19-05	1	BC25882_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25882	Sample	BC25882	targeted	Present	Sample	Mgig_Bot2012_COI	T19-05	COI	45	BC25882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.52786
BC25883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96305	WGS84	BC25883	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.999	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25883_Cmae_Bot2011_COI	T19-06	1	BC25883_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25883	Sample	BC25883	targeted	Present	Sample	Cmae_Bot2011_COI	T19-06	COI	45	BC25883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-06	ATGAACAGTCTATCCTCCTTTAG	147.53847
BC25885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95987	WGS84	BC25885	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.963	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25885_Aamu_Bax2006_COI	T19-08	1	BC25885_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25885	Sample	BC25885	targeted	Present	Sample	Aamu_Bax2006_COI	T19-08	COI	45	BC25885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-08	GCACAACCGGGATCTTTACTTC	147.52361
BC25885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95987	WGS84	BC25885	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25885_Pcan_Bot2011_IGS	T19-08	1	BC25885_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25885	Sample	BC25885	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-08	IGS	45	BC25885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-08	CGTAATCCTCAGTACTGGCTA	147.52361
BC25885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95987	WGS84	BC25885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25885_Sspa_Oph2007_COI	T19-08	1	BC25885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25885	Sample	BC25885	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-08	COI	45	BC25885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-08	GAAGGCCTTCCGATGATC	147.52361
BC25885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95987	WGS84	BC25885	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25885_Upin_Bot2011_COI	T19-08	1	BC25885_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25885	Sample	BC25885	targeted	Absent	Sample	Upin_Bot2011_COI	T19-08	COI	45	BC25885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.52361
BC25886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96363	WGS84	BC25886	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25886_Cjap_Sim2018_COI	T19-09	1	BC25886_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25886	Sample	BC25886	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-09	COI	45	BC25886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51932
BC25886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96363	WGS84	BC25886	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.25	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	18/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25886_Mgig_Bot2012_COI	T19-09	1	BC25886_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25886	Sample	BC25886	targeted	Present	Sample	Mgig_Bot2012_COI	T19-09	COI	45	BC25886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.51932
BC25893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80378	WGS84	BC25893	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25893_Cjap_Sim2018_COI	T19-16	1	BC25893_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25893	Sample	BC25893	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-16	COI	45	BC25893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.5254
BC25893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80378	WGS84	BC25893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.616	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	17/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25893_Mgig_Bot2012_COI	T19-16	1	BC25893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25893	Sample	BC25893	targeted	Present	Sample	Mgig_Bot2012_COI	T19-16	COI	45	BC25893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.5254
BC25894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80318	WGS84	BC25894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25894_Cmae_Bot2011_COI	T19-17	1	BC25894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25894	Sample	BC25894	targeted	Absent	Sample	Cmae_Bot2011_COI	T19-17	COI	45	BC25894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-17	ATGAACAGTCTATCCTCCTTTAG	147.46501
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29597_Cmae_Bot2011_COI	KEMB1	1	BC29597_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	ATGAACAGTCTATCCTCCTTTAG	150.90962
BC29597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47322	WGS84	BC29597	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29597_Msal_Bot2012_COI	KEMB1	1	BC29597_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29597	Sample	BC29597	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB1	COI	45	BC29597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB1	GYTAGTTCCRATGATGTTAGCTG	150.90962
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29599_Aamu_Bax2006_COI	KEMB3	1	BC29599_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	GCACAACCGGGATCTTTACTTC	150.9113
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29599_Pcan_Bot2011_IGS	KEMB3	1	BC29599_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB3	IGS	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	CGTAATCCTCAGTACTGGCTA	150.9113
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29599_Sspa_Oph2007_COI	KEMB3	1	BC29599_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	GAAGGCCTTCCGATGATC	150.9113
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29599_Upin_Bot2011_COI	KEMB3	1	BC29599_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	CTTTAATTACAGCGTTTTTATTGTTGT	150.9113
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29599_Vgib_Wil2025_COI	KEMB3	1	BC29599_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	CCCCTCTTTACAAGTTCTTTAGGGT	150.9113
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29600_Asen_Wil2025_COI	KEMB4	1	BC29600_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	GTTGTTGTTACAACACATGCTCTTTTG	150.90917
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29600_Mgig_Bot2012_COI	KEMB4	1	BC29600_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	TCTTATTCGTTGGAGACTTTATAACCCT	150.90917
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29601_Cmae_Bot2011_COI	KEMB5	1	BC29601_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	ATGAACAGTCTATCCTCCTTTAG	150.91196
BC29601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46877	WGS84	BC29601	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29601_Msal_Bot2012_COI	KEMB5	1	BC29601_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29601	Sample	BC29601	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB5	COI	45	BC29601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB5	GYTAGTTCCRATGATGTTAGCTG	150.91196
BC29608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25322	WGS84	BC29608	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29608_Mgig_Bot2012_COI	HAYA80	1	BC29608_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29608	Sample	BC29608	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA80	COI	45	BC29608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA80	TCTTATTCGTTGGAGACTTTATAACCCT	149.30928
BC29609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2534	WGS84	BC29609	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29609_Cmae_Bot2011_COI	HAYA79	1	BC29609_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29609	Sample	BC29609	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA79	COI	45	BC29609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA79	ATGAACAGTCTATCCTCCTTTAG	149.30644
BC29609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2534	WGS84	BC29609	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29609_Msal_Bot2012_COI	HAYA79	1	BC29609_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29609	Sample	BC29609	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA79	COI	45	BC29609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA79	GYTAGTTCCRATGATGTTAGCTG	149.30644
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2537	WGS84	BC29610	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29610_Aamu_Bax2006_COI	HAYA78	1	BC29610_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	GCACAACCGGGATCTTTACTTC	149.30538
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2537	WGS84	BC29610	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29610_Pcan_Bot2011_IGS	HAYA78	1	BC29610_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA78	IGS	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	CGTAATCCTCAGTACTGGCTA	149.30538
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2537	WGS84	BC29610	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29610_Sspa_Oph2007_COI	HAYA78	1	BC29610_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	GAAGGCCTTCCGATGATC	149.30538
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2537	WGS84	BC29610	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29610_Upin_Bot2011_COI	HAYA78	1	BC29610_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	CTTTAATTACAGCGTTTTTATTGTTGT	149.30538
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.2537	WGS84	BC29610	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29610_Vgib_Wil2025_COI	HAYA78	1	BC29610_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	CCCCTCTTTACAAGTTCTTTAGGGT	149.30538
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.254	WGS84	BC29611	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29611_Asen_Wil2025_COI	HAYA77	1	BC29611_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	GTTGTTGTTACAACACATGCTCTTTTG	149.31117
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.254	WGS84	BC29611	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29611_Mgig_Bot2012_COI	HAYA77	1	BC29611_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	TCTTATTCGTTGGAGACTTTATAACCCT	149.31117
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25463	WGS84	BC29612	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29612_Cmae_Bot2011_COI	HAYA76	1	BC29612_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	ATGAACAGTCTATCCTCCTTTAG	149.3096
BC29612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25463	WGS84	BC29612	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29612_Msal_Bot2012_COI	HAYA76	1	BC29612_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29612	Sample	BC29612	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA76	COI	45	BC29612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA76	GYTAGTTCCRATGATGTTAGCTG	149.3096
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25515	WGS84	BC29614	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29614_Aamu_Bax2006_COI	HAYA74	1	BC29614_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	GCACAACCGGGATCTTTACTTC	149.30727
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25515	WGS84	BC29614	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29614_Pcan_Bot2011_IGS	HAYA74	1	BC29614_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA74	IGS	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	CGTAATCCTCAGTACTGGCTA	149.30727
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25515	WGS84	BC29614	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29614_Sspa_Oph2007_COI	HAYA74	1	BC29614_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	GAAGGCCTTCCGATGATC	149.30727
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25515	WGS84	BC29614	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29614_Upin_Bot2011_COI	HAYA74	1	BC29614_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	CTTTAATTACAGCGTTTTTATTGTTGT	149.30727
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25515	WGS84	BC29614	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29614_Vgib_Wil2025_COI	HAYA74	1	BC29614_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	CCCCTCTTTACAAGTTCTTTAGGGT	149.30727
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25489	WGS84	BC29615	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29615_Asen_Wil2025_COI	HAYA73	1	BC29615_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	GTTGTTGTTACAACACATGCTCTTTTG	149.30571
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25489	WGS84	BC29615	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29615_Mgig_Bot2012_COI	HAYA73	1	BC29615_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	TCTTATTCGTTGGAGACTTTATAACCCT	149.30571
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25433	WGS84	BC29616	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29616_Cmae_Bot2011_COI	HAYA72	1	BC29616_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	ATGAACAGTCTATCCTCCTTTAG	149.31174
BC29616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25433	WGS84	BC29616	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29616_Msal_Bot2012_COI	HAYA72	1	BC29616_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29616	Sample	BC29616	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA72	COI	45	BC29616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA72	GYTAGTTCCRATGATGTTAGCTG	149.31174
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25551	WGS84	BC29618	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29618_Aamu_Bax2006_COI	HAYA70	1	BC29618_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	GCACAACCGGGATCTTTACTTC	149.30925
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25551	WGS84	BC29618	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29618_Pcan_Bot2011_IGS	HAYA70	1	BC29618_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA70	IGS	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	CGTAATCCTCAGTACTGGCTA	149.30925
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25551	WGS84	BC29618	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29618_Sspa_Oph2007_COI	HAYA70	1	BC29618_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	GAAGGCCTTCCGATGATC	149.30925
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25551	WGS84	BC29618	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29618_Upin_Bot2011_COI	HAYA70	1	BC29618_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	CTTTAATTACAGCGTTTTTATTGTTGT	149.30925
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25551	WGS84	BC29618	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29618_Vgib_Wil2025_COI	HAYA70	1	BC29618_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	CCCCTCTTTACAAGTTCTTTAGGGT	149.30925
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25577	WGS84	BC29619	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29619_Asen_Wil2025_COI	HAYA69	1	BC29619_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	GTTGTTGTTACAACACATGCTCTTTTG	149.30652
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25577	WGS84	BC29619	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29619_Mgig_Bot2012_COI	HAYA69	1	BC29619_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	TCTTATTCGTTGGAGACTTTATAACCCT	149.30652
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25542	WGS84	BC29621	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29621_Aamu_Bax2006_COI	HAYA67	1	BC29621_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	GCACAACCGGGATCTTTACTTC	149.31127
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25542	WGS84	BC29621	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29621_Pcan_Bot2011_IGS	HAYA67	1	BC29621_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA67	IGS	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	CGTAATCCTCAGTACTGGCTA	149.31127
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25542	WGS84	BC29621	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29621_Sspa_Oph2007_COI	HAYA67	1	BC29621_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	GAAGGCCTTCCGATGATC	149.31127
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25542	WGS84	BC29621	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29621_Upin_Bot2011_COI	HAYA67	1	BC29621_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	CTTTAATTACAGCGTTTTTATTGTTGT	149.31127
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25542	WGS84	BC29621	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29621_Vgib_Wil2025_COI	HAYA67	1	BC29621_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	CCCCTCTTTACAAGTTCTTTAGGGT	149.31127
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25536	WGS84	BC29622	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29622_Asen_Wil2025_COI	HAYA66	1	BC29622_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	GTTGTTGTTACAACACATGCTCTTTTG	149.3102
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25536	WGS84	BC29622	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29622_Mgig_Bot2012_COI	HAYA66	1	BC29622_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	TCTTATTCGTTGGAGACTTTATAACCCT	149.3102
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25552	WGS84	BC29623	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29623_Cmae_Bot2011_COI	HAYA65	1	BC29623_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	ATGAACAGTCTATCCTCCTTTAG	149.30799
BC29623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25552	WGS84	BC29623	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29623_Msal_Bot2012_COI	HAYA65	1	BC29623_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29623	Sample	BC29623	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA65	COI	45	BC29623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA65	GYTAGTTCCRATGATGTTAGCTG	149.30799
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25587	WGS84	BC29625	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29625_Aamu_Bax2006_COI	HAYA63	1	BC29625_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	GCACAACCGGGATCTTTACTTC	149.31251
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25587	WGS84	BC29625	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29625_Pcan_Bot2011_IGS	HAYA63	1	BC29625_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA63	IGS	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	CGTAATCCTCAGTACTGGCTA	149.31251
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25587	WGS84	BC29625	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29625_Sspa_Oph2007_COI	HAYA63	1	BC29625_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	GAAGGCCTTCCGATGATC	149.31251
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25587	WGS84	BC29625	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29625_Upin_Bot2011_COI	HAYA63	1	BC29625_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	CTTTAATTACAGCGTTTTTATTGTTGT	149.31251
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25587	WGS84	BC29625	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29625_Vgib_Wil2025_COI	HAYA63	1	BC29625_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	CCCCTCTTTACAAGTTCTTTAGGGT	149.31251
BC29626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25676	WGS84	BC29626	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29626_Mgig_Bot2012_COI	HAYA62	1	BC29626_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29626	Sample	BC29626	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA62	COI	45	BC29626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA62	TCTTATTCGTTGGAGACTTTATAACCCT	149.31127
BC29627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25593	WGS84	BC29627	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29627_Cmae_Bot2011_COI	HAYA61	1	BC29627_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29627	Sample	BC29627	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA61	COI	45	BC29627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA61	ATGAACAGTCTATCCTCCTTTAG	149.31054
BC29627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25593	WGS84	BC29627	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29627_Msal_Bot2012_COI	HAYA61	1	BC29627_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29627	Sample	BC29627	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA61	COI	45	BC29627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA61	GYTAGTTCCRATGATGTTAGCTG	149.31054
BC29629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25645	WGS84	BC29629	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29629_Aamu_Bax2006_COI	HAYA59	1	BC29629_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29629	Sample	BC29629	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA59	COI	45	BC29629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA59	GCACAACCGGGATCTTTACTTC	149.30918
BC29629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25645	WGS84	BC29629	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29629_Pcan_Bot2011_IGS	HAYA59	1	BC29629_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29629	Sample	BC29629	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA59	IGS	45	BC29629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA59	CGTAATCCTCAGTACTGGCTA	149.30918
BC29629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25645	WGS84	BC29629	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29629_Sspa_Oph2007_COI	HAYA59	1	BC29629_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29629	Sample	BC29629	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA59	COI	45	BC29629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA59	GAAGGCCTTCCGATGATC	149.30918
BC29629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25645	WGS84	BC29629	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29629_Upin_Bot2011_COI	HAYA59	1	BC29629_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29629	Sample	BC29629	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA59	COI	45	BC29629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA59	CTTTAATTACAGCGTTTTTATTGTTGT	149.30918
BC29630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2565	WGS84	BC29630	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29630_Cmae_Bot2011_COI	HAYA58	1	BC29630_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29630	Sample	BC29630	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA58	COI	45	BC29630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA58	ATGAACAGTCTATCCTCCTTTAG	149.30741
BC29630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2565	WGS84	BC29630	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29630_Msal_Bot2012_COI	HAYA58	1	BC29630_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29630	Sample	BC29630	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA58	COI	45	BC29630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA58	GYTAGTTCCRATGATGTTAGCTG	149.30741
BC29632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25737	WGS84	BC29632	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29632_Aamu_Bax2006_COI	HAYA56	1	BC29632_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29632	Sample	BC29632	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA56	COI	45	BC29632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA56	GCACAACCGGGATCTTTACTTC	149.31061
BC29632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25737	WGS84	BC29632	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29632_Pcan_Bot2011_IGS	HAYA56	1	BC29632_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29632	Sample	BC29632	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA56	IGS	45	BC29632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA56	CGTAATCCTCAGTACTGGCTA	149.31061
BC29632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25737	WGS84	BC29632	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29632_Sspa_Oph2007_COI	HAYA56	1	BC29632_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29632	Sample	BC29632	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA56	COI	45	BC29632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA56	GAAGGCCTTCCGATGATC	149.31061
BC29632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25737	WGS84	BC29632	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29632_Upin_Bot2011_COI	HAYA56	1	BC29632_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29632	Sample	BC29632	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA56	COI	45	BC29632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA56	CTTTAATTACAGCGTTTTTATTGTTGT	149.31061
BC29633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25669	WGS84	BC29633	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29633_Mgig_Bot2012_COI	HAYA55	1	BC29633_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29633	Sample	BC29633	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA55	COI	45	BC29633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA55	TCTTATTCGTTGGAGACTTTATAACCCT	149.31006
BC29634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25781	WGS84	BC29634	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29634_Cmae_Bot2011_COI	HAYA54	1	BC29634_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29634	Sample	BC29634	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA54	COI	45	BC29634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA54	ATGAACAGTCTATCCTCCTTTAG	149.30938
BC29634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25781	WGS84	BC29634	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29634_Msal_Bot2012_COI	HAYA54	1	BC29634_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29634	Sample	BC29634	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA54	COI	45	BC29634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA54	GYTAGTTCCRATGATGTTAGCTG	149.30938
BC29636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25735	WGS84	BC29636	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29636_Aamu_Bax2006_COI	HAYA52	1	BC29636_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29636	Sample	BC29636	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA52	COI	45	BC29636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA52	GCACAACCGGGATCTTTACTTC	149.31285
BC29636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25735	WGS84	BC29636	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29636_Pcan_Bot2011_IGS	HAYA52	1	BC29636_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29636	Sample	BC29636	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA52	IGS	45	BC29636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA52	CGTAATCCTCAGTACTGGCTA	149.31285
BC29636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25735	WGS84	BC29636	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29636_Sspa_Oph2007_COI	HAYA52	1	BC29636_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29636	Sample	BC29636	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA52	COI	45	BC29636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA52	GAAGGCCTTCCGATGATC	149.31285
BC29636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25735	WGS84	BC29636	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29636_Upin_Bot2011_COI	HAYA52	1	BC29636_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29636	Sample	BC29636	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA52	COI	45	BC29636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA52	CTTTAATTACAGCGTTTTTATTGTTGT	149.31285
BC29637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25823	WGS84	BC29637	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29637_Mgig_Bot2012_COI	HAYA51	1	BC29637_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29637	Sample	BC29637	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA51	COI	45	BC29637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA51	TCTTATTCGTTGGAGACTTTATAACCCT	149.31177
BC29638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25789	WGS84	BC29638	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29638_Cmae_Bot2011_COI	HAYA50	1	BC29638_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29638	Sample	BC29638	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA50	COI	45	BC29638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA50	ATGAACAGTCTATCCTCCTTTAG	149.30852
BC29638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25789	WGS84	BC29638	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29638_Msal_Bot2012_COI	HAYA50	1	BC29638_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29638	Sample	BC29638	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA50	COI	45	BC29638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA50	GYTAGTTCCRATGATGTTAGCTG	149.30852
BC29640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25897	WGS84	BC29640	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29640_Mgig_Bot2012_COI	HAYA48	1	BC29640_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29640	Sample	BC29640	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA48	COI	45	BC29640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA48	TCTTATTCGTTGGAGACTTTATAACCCT	149.31222
BC29641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25844	WGS84	BC29641	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29641_Cmae_Bot2011_COI	HAYA47	1	BC29641_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29641	Sample	BC29641	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA47	COI	45	BC29641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA47	ATGAACAGTCTATCCTCCTTTAG	149.30923
BC29641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25844	WGS84	BC29641	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29641_Msal_Bot2012_COI	HAYA47	1	BC29641_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29641	Sample	BC29641	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA47	COI	45	BC29641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA47	GYTAGTTCCRATGATGTTAGCTG	149.30923
BC29643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25875	WGS84	BC29643	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29643_Aamu_Bax2006_COI	HAYA45	1	BC29643_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29643	Sample	BC29643	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA45	COI	45	BC29643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA45	GCACAACCGGGATCTTTACTTC	149.31316
BC29643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25875	WGS84	BC29643	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29643_Pcan_Bot2011_IGS	HAYA45	1	BC29643_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29643	Sample	BC29643	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA45	IGS	45	BC29643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA45	CGTAATCCTCAGTACTGGCTA	149.31316
BC29643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25875	WGS84	BC29643	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29643_Sspa_Oph2007_COI	HAYA45	1	BC29643_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29643	Sample	BC29643	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA45	COI	45	BC29643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA45	GAAGGCCTTCCGATGATC	149.31316
BC29643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25875	WGS84	BC29643	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29643_Upin_Bot2011_COI	HAYA45	1	BC29643_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29643	Sample	BC29643	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA45	COI	45	BC29643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA45	CTTTAATTACAGCGTTTTTATTGTTGT	149.31316
BC29644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25974	WGS84	BC29644	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29644_Mgig_Bot2012_COI	HAYA44	1	BC29644_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29644	Sample	BC29644	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA44	COI	45	BC29644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA44	TCTTATTCGTTGGAGACTTTATAACCCT	149.31053
BC29645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25954	WGS84	BC29645	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29645_Cmae_Bot2011_COI	HAYA43	1	BC29645_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29645	Sample	BC29645	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA43	COI	45	BC29645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA43	ATGAACAGTCTATCCTCCTTTAG	149.30874
BC29645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25954	WGS84	BC29645	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29645_Msal_Bot2012_COI	HAYA43	1	BC29645_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29645	Sample	BC29645	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA43	COI	45	BC29645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA43	GYTAGTTCCRATGATGTTAGCTG	149.30874
BC29647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26013	WGS84	BC29647	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29647_Aamu_Bax2006_COI	HAYA41	1	BC29647_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29647	Sample	BC29647	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA41	COI	45	BC29647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA41	GCACAACCGGGATCTTTACTTC	149.31281
BC29647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26013	WGS84	BC29647	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29647_Pcan_Bot2011_IGS	HAYA41	1	BC29647_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29647	Sample	BC29647	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA41	IGS	45	BC29647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA41	CGTAATCCTCAGTACTGGCTA	149.31281
BC29647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26013	WGS84	BC29647	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29647_Sspa_Oph2007_COI	HAYA41	1	BC29647_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29647	Sample	BC29647	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA41	COI	45	BC29647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA41	GAAGGCCTTCCGATGATC	149.31281
BC29647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26013	WGS84	BC29647	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29647_Upin_Bot2011_COI	HAYA41	1	BC29647_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29647	Sample	BC29647	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA41	COI	45	BC29647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA41	CTTTAATTACAGCGTTTTTATTGTTGT	149.31281
BC29648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25966	WGS84	BC29648	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29648_Mgig_Bot2012_COI	HAYA40	1	BC29648_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29648	Sample	BC29648	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA40	COI	45	BC29648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA40	TCTTATTCGTTGGAGACTTTATAACCCT	149.31048
BC29649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26015	WGS84	BC29649	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29649_Cmae_Bot2011_COI	HAYA39	1	BC29649_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29649	Sample	BC29649	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA39	COI	45	BC29649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA39	ATGAACAGTCTATCCTCCTTTAG	149.30916
BC29649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26015	WGS84	BC29649	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29649_Msal_Bot2012_COI	HAYA39	1	BC29649_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29649	Sample	BC29649	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA39	COI	45	BC29649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA39	GYTAGTTCCRATGATGTTAGCTG	149.30916
BC29650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26105	WGS84	BC29650	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29650_Aamu_Bax2006_COI	HAYA38	1	BC29650_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29650	Sample	BC29650	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA38	COI	45	BC29650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA38	GCACAACCGGGATCTTTACTTC	149.31414
BC29650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26105	WGS84	BC29650	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29650_Pcan_Bot2011_IGS	HAYA38	1	BC29650_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29650	Sample	BC29650	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA38	IGS	45	BC29650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA38	CGTAATCCTCAGTACTGGCTA	149.31414
BC29650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26105	WGS84	BC29650	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29650_Sspa_Oph2007_COI	HAYA38	1	BC29650_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29650	Sample	BC29650	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA38	COI	45	BC29650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA38	GAAGGCCTTCCGATGATC	149.31414
BC29650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26105	WGS84	BC29650	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29650_Upin_Bot2011_COI	HAYA38	1	BC29650_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29650	Sample	BC29650	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA38	COI	45	BC29650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA38	CTTTAATTACAGCGTTTTTATTGTTGT	149.31414
BC29651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26007	WGS84	BC29651	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29651_Mgig_Bot2012_COI	HAYA37	1	BC29651_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29651	Sample	BC29651	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA37	COI	45	BC29651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA37	TCTTATTCGTTGGAGACTTTATAACCCT	149.31273
BC29652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26079	WGS84	BC29652	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29652_Cmae_Bot2011_COI	HAYA36	1	BC29652_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29652	Sample	BC29652	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA36	COI	45	BC29652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA36	ATGAACAGTCTATCCTCCTTTAG	149.31151
BC29652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26079	WGS84	BC29652	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29652_Msal_Bot2012_COI	HAYA36	1	BC29652_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29652	Sample	BC29652	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA36	COI	45	BC29652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA36	GYTAGTTCCRATGATGTTAGCTG	149.31151
BC29654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26084	WGS84	BC29654	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29654_Aamu_Bax2006_COI	HAYA34	1	BC29654_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29654	Sample	BC29654	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA34	COI	45	BC29654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA34	GCACAACCGGGATCTTTACTTC	149.31405
BC29654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26084	WGS84	BC29654	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29654_Pcan_Bot2011_IGS	HAYA34	1	BC29654_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29654	Sample	BC29654	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA34	IGS	45	BC29654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA34	CGTAATCCTCAGTACTGGCTA	149.31405
BC29654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26084	WGS84	BC29654	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29654_Sspa_Oph2007_COI	HAYA34	1	BC29654_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29654	Sample	BC29654	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA34	COI	45	BC29654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA34	GAAGGCCTTCCGATGATC	149.31405
BC29654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26084	WGS84	BC29654	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29654_Upin_Bot2011_COI	HAYA34	1	BC29654_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29654	Sample	BC29654	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA34	COI	45	BC29654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA34	CTTTAATTACAGCGTTTTTATTGTTGT	149.31405
BC29655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26145	WGS84	BC29655	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29655_Mgig_Bot2012_COI	HAYA33	1	BC29655_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29655	Sample	BC29655	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA33	COI	45	BC29655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA33	TCTTATTCGTTGGAGACTTTATAACCCT	149.31383
BC29656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26191	WGS84	BC29656	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29656_Cmae_Bot2011_COI	HAYA32	1	BC29656_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29656	Sample	BC29656	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA32	COI	45	BC29656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA32	ATGAACAGTCTATCCTCCTTTAG	149.31036
BC29656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26191	WGS84	BC29656	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29656_Msal_Bot2012_COI	HAYA32	1	BC29656_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29656	Sample	BC29656	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA32	COI	45	BC29656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA32	GYTAGTTCCRATGATGTTAGCTG	149.31036
BC29658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26249	WGS84	BC29658	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29658_Aamu_Bax2006_COI	HAYA30	1	BC29658_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29658	Sample	BC29658	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA30	COI	45	BC29658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA30	GCACAACCGGGATCTTTACTTC	149.31419
BC29658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26249	WGS84	BC29658	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29658_Pcan_Bot2011_IGS	HAYA30	1	BC29658_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29658	Sample	BC29658	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA30	IGS	45	BC29658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA30	CGTAATCCTCAGTACTGGCTA	149.31419
BC29658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26249	WGS84	BC29658	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29658_Sspa_Oph2007_COI	HAYA30	1	BC29658_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29658	Sample	BC29658	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA30	COI	45	BC29658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA30	GAAGGCCTTCCGATGATC	149.31419
BC29658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26249	WGS84	BC29658	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29658_Upin_Bot2011_COI	HAYA30	1	BC29658_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29658	Sample	BC29658	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA30	COI	45	BC29658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA30	CTTTAATTACAGCGTTTTTATTGTTGT	149.31419
BC29659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26259	WGS84	BC29659	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29659_Mgig_Bot2012_COI	HAYA29	1	BC29659_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29659	Sample	BC29659	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA29	COI	45	BC29659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA29	TCTTATTCGTTGGAGACTTTATAACCCT	149.31503
BC29661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26326	WGS84	BC29661	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29661_Aamu_Bax2006_COI	HAYA27	1	BC29661_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29661	Sample	BC29661	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA27	COI	45	BC29661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA27	GCACAACCGGGATCTTTACTTC	149.31547
BC29661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26326	WGS84	BC29661	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29661_Pcan_Bot2011_IGS	HAYA27	1	BC29661_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29661	Sample	BC29661	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA27	IGS	45	BC29661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA27	CGTAATCCTCAGTACTGGCTA	149.31547
BC29661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26326	WGS84	BC29661	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29661_Sspa_Oph2007_COI	HAYA27	1	BC29661_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29661	Sample	BC29661	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA27	COI	45	BC29661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA27	GAAGGCCTTCCGATGATC	149.31547
BC29661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26326	WGS84	BC29661	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29661_Upin_Bot2011_COI	HAYA27	1	BC29661_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29661	Sample	BC29661	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA27	COI	45	BC29661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA27	CTTTAATTACAGCGTTTTTATTGTTGT	149.31547
BC29662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26291	WGS84	BC29662	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29662_Mgig_Bot2012_COI	HAYA26	1	BC29662_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29662	Sample	BC29662	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA26	COI	45	BC29662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA26	TCTTATTCGTTGGAGACTTTATAACCCT	149.31415
BC29663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26424	WGS84	BC29663	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29663_Cmae_Bot2011_COI	HAYA25	1	BC29663_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29663	Sample	BC29663	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA25	COI	45	BC29663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA25	ATGAACAGTCTATCCTCCTTTAG	149.3158
BC29663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26424	WGS84	BC29663	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29663_Msal_Bot2012_COI	HAYA25	1	BC29663_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29663	Sample	BC29663	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA25	COI	45	BC29663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA25	GYTAGTTCCRATGATGTTAGCTG	149.3158
BC29665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26359	WGS84	BC29665	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29665_Aamu_Bax2006_COI	HAYA23	1	BC29665_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29665	Sample	BC29665	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA23	COI	45	BC29665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA23	GCACAACCGGGATCTTTACTTC	149.31395
BC29665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26359	WGS84	BC29665	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29665_Pcan_Bot2011_IGS	HAYA23	1	BC29665_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29665	Sample	BC29665	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA23	IGS	45	BC29665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA23	CGTAATCCTCAGTACTGGCTA	149.31395
BC29665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26359	WGS84	BC29665	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29665_Sspa_Oph2007_COI	HAYA23	1	BC29665_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29665	Sample	BC29665	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA23	COI	45	BC29665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA23	GAAGGCCTTCCGATGATC	149.31395
BC29665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26359	WGS84	BC29665	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29665_Upin_Bot2011_COI	HAYA23	1	BC29665_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29665	Sample	BC29665	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA23	COI	45	BC29665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA23	CTTTAATTACAGCGTTTTTATTGTTGT	149.31395
BC29666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26398	WGS84	BC29666	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29666_Mgig_Bot2012_COI	HAYA22	1	BC29666_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29666	Sample	BC29666	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA22	COI	45	BC29666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA22	TCTTATTCGTTGGAGACTTTATAACCCT	149.31106
BC29667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26507	WGS84	BC29667	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29667_Cmae_Bot2011_COI	HAYA21	1	BC29667_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29667	Sample	BC29667	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA21	COI	45	BC29667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA21	ATGAACAGTCTATCCTCCTTTAG	149.31557
BC29667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26507	WGS84	BC29667	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29667_Msal_Bot2012_COI	HAYA21	1	BC29667_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29667	Sample	BC29667	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA21	COI	45	BC29667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA21	GYTAGTTCCRATGATGTTAGCTG	149.31557
BC29669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26538	WGS84	BC29669	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29669_Aamu_Bax2006_COI	HAYA19	1	BC29669_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29669	Sample	BC29669	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA19	COI	45	BC29669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA19	GCACAACCGGGATCTTTACTTC	149.3153
BC29669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26538	WGS84	BC29669	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29669_Pcan_Bot2011_IGS	HAYA19	1	BC29669_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29669	Sample	BC29669	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA19	IGS	45	BC29669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA19	CGTAATCCTCAGTACTGGCTA	149.3153
BC29669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26538	WGS84	BC29669	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29669_Sspa_Oph2007_COI	HAYA19	1	BC29669_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29669	Sample	BC29669	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA19	COI	45	BC29669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA19	GAAGGCCTTCCGATGATC	149.3153
BC29669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26538	WGS84	BC29669	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29669_Upin_Bot2011_COI	HAYA19	1	BC29669_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29669	Sample	BC29669	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA19	COI	45	BC29669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA19	CTTTAATTACAGCGTTTTTATTGTTGT	149.3153
BC29670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26505	WGS84	BC29670	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29670_Cmae_Bot2011_COI	HAYA18	1	BC29670_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29670	Sample	BC29670	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA18	COI	45	BC29670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA18	ATGAACAGTCTATCCTCCTTTAG	149.31493
BC29670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26505	WGS84	BC29670	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29670_Msal_Bot2012_COI	HAYA18	1	BC29670_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29670	Sample	BC29670	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA18	COI	45	BC29670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA18	GYTAGTTCCRATGATGTTAGCTG	149.31493
BC29672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26587	WGS84	BC29672	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29672_Aamu_Bax2006_COI	HAYA16	1	BC29672_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29672	Sample	BC29672	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA16	COI	45	BC29672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA16	GCACAACCGGGATCTTTACTTC	149.3118
BC29672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26587	WGS84	BC29672	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29672_Pcan_Bot2011_IGS	HAYA16	1	BC29672_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29672	Sample	BC29672	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA16	IGS	45	BC29672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA16	CGTAATCCTCAGTACTGGCTA	149.3118
BC29672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26587	WGS84	BC29672	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29672_Sspa_Oph2007_COI	HAYA16	1	BC29672_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29672	Sample	BC29672	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA16	COI	45	BC29672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA16	GAAGGCCTTCCGATGATC	149.3118
BC29672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26587	WGS84	BC29672	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29672_Upin_Bot2011_COI	HAYA16	1	BC29672_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29672	Sample	BC29672	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA16	COI	45	BC29672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA16	CTTTAATTACAGCGTTTTTATTGTTGT	149.3118
BC29673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26654	WGS84	BC29673	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29673_Mgig_Bot2012_COI	HAYA15	1	BC29673_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29673	Sample	BC29673	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA15	COI	45	BC29673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA15	TCTTATTCGTTGGAGACTTTATAACCCT	149.31624
BC29674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26576	WGS84	BC29674	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29674_Cmae_Bot2011_COI	HAYA14	1	BC29674_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29674	Sample	BC29674	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA14	COI	45	BC29674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA14	ATGAACAGTCTATCCTCCTTTAG	149.31553
BC29674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26576	WGS84	BC29674	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29674_Msal_Bot2012_COI	HAYA14	1	BC29674_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29674	Sample	BC29674	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA14	COI	45	BC29674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA14	GYTAGTTCCRATGATGTTAGCTG	149.31553
BC29676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29676	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29676_Aamu_Bax2006_COI	HAYA12	1	BC29676_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29676	Sample	BC29676	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA12	COI	45	BC29676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA12	GCACAACCGGGATCTTTACTTC	149.31633
BC29676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29676	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29676_Pcan_Bot2011_IGS	HAYA12	1	BC29676_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29676	Sample	BC29676	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA12	IGS	45	BC29676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA12	CGTAATCCTCAGTACTGGCTA	149.31633
BC29676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29676	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29676_Sspa_Oph2007_COI	HAYA12	1	BC29676_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29676	Sample	BC29676	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA12	COI	45	BC29676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA12	GAAGGCCTTCCGATGATC	149.31633
BC29676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29676	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29676_Upin_Bot2011_COI	HAYA12	1	BC29676_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29676	Sample	BC29676	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA12	COI	45	BC29676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA12	CTTTAATTACAGCGTTTTTATTGTTGT	149.31633
BC29677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26725	WGS84	BC29677	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29677_Mgig_Bot2012_COI	HAYA11	1	BC29677_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29677	Sample	BC29677	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA11	COI	45	BC29677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA11	TCTTATTCGTTGGAGACTTTATAACCCT	149.31629
BC29678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26727	WGS84	BC29678	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29678_Cmae_Bot2011_COI	HAYA10	1	BC29678_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29678	Sample	BC29678	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA10	COI	45	BC29678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA10	ATGAACAGTCTATCCTCCTTTAG	149.31534
BC29678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26727	WGS84	BC29678	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29678_Msal_Bot2012_COI	HAYA10	1	BC29678_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29678	Sample	BC29678	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA10	COI	45	BC29678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA10	GYTAGTTCCRATGATGTTAGCTG	149.31534
BC29680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26761	WGS84	BC29680	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29680_Mgig_Bot2012_COI	HAYA8	1	BC29680_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29680	Sample	BC29680	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA8	COI	45	BC29680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA8	TCTTATTCGTTGGAGACTTTATAACCCT	149.31205
BC29681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26785	WGS84	BC29681	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29681_Cmae_Bot2011_COI	HAYA7	1	BC29681_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29681	Sample	BC29681	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA7	COI	45	BC29681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA7	ATGAACAGTCTATCCTCCTTTAG	149.31684
BC29681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26785	WGS84	BC29681	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29681_Msal_Bot2012_COI	HAYA7	1	BC29681_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29681	Sample	BC29681	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA7	COI	45	BC29681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA7	GYTAGTTCCRATGATGTTAGCTG	149.31684
BC29683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2681	WGS84	BC29683	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29683_Aamu_Bax2006_COI	HAYA5	1	BC29683_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29683	Sample	BC29683	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA5	COI	45	BC29683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA5	GCACAACCGGGATCTTTACTTC	149.31408
BC29683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2681	WGS84	BC29683	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29683_Pcan_Bot2011_IGS	HAYA5	1	BC29683_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29683	Sample	BC29683	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA5	IGS	45	BC29683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA5	CGTAATCCTCAGTACTGGCTA	149.31408
BC29683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2681	WGS84	BC29683	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29683_Sspa_Oph2007_COI	HAYA5	1	BC29683_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29683	Sample	BC29683	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA5	COI	45	BC29683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA5	GAAGGCCTTCCGATGATC	149.31408
BC29683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2681	WGS84	BC29683	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29683_Upin_Bot2011_COI	HAYA5	1	BC29683_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29683	Sample	BC29683	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA5	COI	45	BC29683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA5	CTTTAATTACAGCGTTTTTATTGTTGT	149.31408
BC29684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26749	WGS84	BC29684	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29684_Mgig_Bot2012_COI	HAYA4	1	BC29684_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29684	Sample	BC29684	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA4	COI	45	BC29684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA4	TCTTATTCGTTGGAGACTTTATAACCCT	149.3128
BC29685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26771	WGS84	BC29685	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29685_Cmae_Bot2011_COI	HAYA3	1	BC29685_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29685	Sample	BC29685	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA3	COI	45	BC29685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA3	ATGAACAGTCTATCCTCCTTTAG	149.31608
BC29685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26771	WGS84	BC29685	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29685_Msal_Bot2012_COI	HAYA3	1	BC29685_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29685	Sample	BC29685	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA3	COI	45	BC29685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA3	GYTAGTTCCRATGATGTTAGCTG	149.31608
BC29687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26989	WGS84	BC29687	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29687_Aamu_Bax2006_COI	HAYA1	1	BC29687_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29687	Sample	BC29687	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA1	COI	45	BC29687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA1	GCACAACCGGGATCTTTACTTC	149.31431
BC29687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26989	WGS84	BC29687	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29687_Pcan_Bot2011_IGS	HAYA1	1	BC29687_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29687	Sample	BC29687	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA1	IGS	45	BC29687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA1	CGTAATCCTCAGTACTGGCTA	149.31431
BC29687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26989	WGS84	BC29687	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29687_Sspa_Oph2007_COI	HAYA1	1	BC29687_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29687	Sample	BC29687	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA1	COI	45	BC29687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA1	GAAGGCCTTCCGATGATC	149.31431
BC29687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26989	WGS84	BC29687	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29687_Upin_Bot2011_COI	HAYA1	1	BC29687_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29687	Sample	BC29687	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA1	COI	45	BC29687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA1	CTTTAATTACAGCGTTTTTATTGTTGT	149.31431
BC29688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18009	WGS84	BC29688	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.366	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29688_Mgig_Bot2012_COI	DEVA19	1	BC29688_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29688	Sample	BC29688	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA19	COI	45	BC29688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA19	TCTTATTCGTTGGAGACTTTATAACCCT	146.3645
BC29689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18175	WGS84	BC29689	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29689_Cmae_Bot2011_COI	DEVA18	1	BC29689_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29689	Sample	BC29689	targeted	Absent	Sample	Cmae_Bot2011_COI	DEVA18	COI	45	BC29689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA18	ATGAACAGTCTATCCTCCTTTAG	146.36453
BC29689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18175	WGS84	BC29689	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29689_Msal_Bot2012_COI	DEVA18	1	BC29689_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29689	Sample	BC29689	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA18	COI	45	BC29689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA18	GYTAGTTCCRATGATGTTAGCTG	146.36453
BC29690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18121	WGS84	BC29690	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29690_Aamu_Bax2006_COI	DEVA17	1	BC29690_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29690	Sample	BC29690	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA17	COI	45	BC29690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA17	GCACAACCGGGATCTTTACTTC	146.36505
BC29690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18121	WGS84	BC29690	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29690_Pcan_Bot2011_IGS	DEVA17	1	BC29690_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29690	Sample	BC29690	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA17	IGS	45	BC29690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA17	CGTAATCCTCAGTACTGGCTA	146.36505
BC29690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18121	WGS84	BC29690	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29690_Sspa_Oph2007_COI	DEVA17	1	BC29690_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29690	Sample	BC29690	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA17	COI	45	BC29690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA17	GAAGGCCTTCCGATGATC	146.36505
BC29690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18121	WGS84	BC29690	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29690_Upin_Bot2011_COI	DEVA17	1	BC29690_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29690	Sample	BC29690	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA17	COI	45	BC29690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA17	CTTTAATTACAGCGTTTTTATTGTTGT	146.36505
BC29692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18176	WGS84	BC29692	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29692_Cmae_Bot2011_COI	DEVA16	1	BC29692_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29692	Sample	BC29692	targeted	Absent	Sample	Cmae_Bot2011_COI	DEVA16	COI	45	BC29692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA16	ATGAACAGTCTATCCTCCTTTAG	146.36377
BC29692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18176	WGS84	BC29692	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29692_Msal_Bot2012_COI	DEVA16	1	BC29692_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29692	Sample	BC29692	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA16	COI	45	BC29692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA16	GYTAGTTCCRATGATGTTAGCTG	146.36377
BC29694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18351	WGS84	BC29694	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29694_Aamu_Bax2006_COI	DEVA13	1	BC29694_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29694	Sample	BC29694	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA13	COI	45	BC29694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA13	GCACAACCGGGATCTTTACTTC	146.36547
BC29694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18351	WGS84	BC29694	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29694_Pcan_Bot2011_IGS	DEVA13	1	BC29694_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29694	Sample	BC29694	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA13	IGS	45	BC29694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA13	CGTAATCCTCAGTACTGGCTA	146.36547
BC29694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18351	WGS84	BC29694	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29694_Sspa_Oph2007_COI	DEVA13	1	BC29694_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29694	Sample	BC29694	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA13	COI	45	BC29694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA13	GAAGGCCTTCCGATGATC	146.36547
BC29694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18351	WGS84	BC29694	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29694_Upin_Bot2011_COI	DEVA13	1	BC29694_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29694	Sample	BC29694	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA13	COI	45	BC29694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA13	CTTTAATTACAGCGTTTTTATTGTTGT	146.36547
BC29695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18368	WGS84	BC29695	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.358	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29695_Mgig_Bot2012_COI	DEVA12	1	BC29695_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29695	Sample	BC29695	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA12	COI	45	BC29695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA12	TCTTATTCGTTGGAGACTTTATAACCCT	146.36384
BC29696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18545	WGS84	BC29696	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29696_Cmae_Bot2011_COI	DEVA10	1	BC29696_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29696	Sample	BC29696	targeted	Absent	Sample	Cmae_Bot2011_COI	DEVA10	COI	45	BC29696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA10	ATGAACAGTCTATCCTCCTTTAG	146.36573
BC29696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18545	WGS84	BC29696	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29696_Msal_Bot2012_COI	DEVA10	1	BC29696_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29696	Sample	BC29696	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA10	COI	45	BC29696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA10	GYTAGTTCCRATGATGTTAGCTG	146.36573
BC29698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18654	WGS84	BC29698	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29698_Aamu_Bax2006_COI	DEVA8	1	BC29698_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29698	Sample	BC29698	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA8	COI	45	BC29698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA8	GCACAACCGGGATCTTTACTTC	146.36659
BC29698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18654	WGS84	BC29698	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29698_Pcan_Bot2011_IGS	DEVA8	1	BC29698_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29698	Sample	BC29698	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA8	IGS	45	BC29698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA8	CGTAATCCTCAGTACTGGCTA	146.36659
BC29698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18654	WGS84	BC29698	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29698_Sspa_Oph2007_COI	DEVA8	1	BC29698_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29698	Sample	BC29698	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA8	COI	45	BC29698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA8	GAAGGCCTTCCGATGATC	146.36659
BC29698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18654	WGS84	BC29698	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29698_Upin_Bot2011_COI	DEVA8	1	BC29698_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29698	Sample	BC29698	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA8	COI	45	BC29698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA8	CTTTAATTACAGCGTTTTTATTGTTGT	146.36659
BC29699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18728	WGS84	BC29699	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.814	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29699_Mgig_Bot2012_COI	DEVA6	1	BC29699_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29699	Sample	BC29699	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA6	COI	45	BC29699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA6	TCTTATTCGTTGGAGACTTTATAACCCT	146.36737
BC29700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1878	WGS84	BC29700	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.269	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29700_Cmae_Bot2011_COI	DEVA5	1	BC29700_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29700	Sample	BC29700	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA5	COI	45	BC29700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA5	ATGAACAGTCTATCCTCCTTTAG	146.3656
BC29700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1878	WGS84	BC29700	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29700_Msal_Bot2012_COI	DEVA5	1	BC29700_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29700	Sample	BC29700	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA5	COI	45	BC29700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA5	GYTAGTTCCRATGATGTTAGCTG	146.3656
BC29702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18922	WGS84	BC29702	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29702_Aamu_Bax2006_COI	DEVA3	1	BC29702_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29702	Sample	BC29702	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA3	COI	45	BC29702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA3	GCACAACCGGGATCTTTACTTC	146.3675
BC29702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18922	WGS84	BC29702	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29702_Pcan_Bot2011_IGS	DEVA3	1	BC29702_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29702	Sample	BC29702	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA3	IGS	45	BC29702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA3	CGTAATCCTCAGTACTGGCTA	146.3675
BC29702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18922	WGS84	BC29702	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29702_Sspa_Oph2007_COI	DEVA3	1	BC29702_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29702	Sample	BC29702	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA3	COI	45	BC29702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA3	GAAGGCCTTCCGATGATC	146.3675
BC29702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18922	WGS84	BC29702	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29702_Upin_Bot2011_COI	DEVA3	1	BC29702_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29702	Sample	BC29702	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA3	COI	45	BC29702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA3	CTTTAATTACAGCGTTTTTATTGTTGT	146.3675
BC29703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.19141	WGS84	BC29703	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.127	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29703_Mgig_Bot2012_COI	DEVA1	1	BC29703_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29703	Sample	BC29703	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA1	COI	45	BC29703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA1	TCTTATTCGTTGGAGACTTTATAACCCT	146.36788
BC29704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8817	WGS84	BC29704	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29704_Cmae_Bot2011_COI	NEWA45	1	BC29704_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29704	Sample	BC29704	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA45	COI	45	BC29704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA45	ATGAACAGTCTATCCTCCTTTAG	151.7493
BC29704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8817	WGS84	BC29704	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29704_Msal_Bot2012_COI	NEWA45	1	BC29704_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29704	Sample	BC29704	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA45	COI	45	BC29704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA45	GYTAGTTCCRATGATGTTAGCTG	151.7493
BC29706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88377	WGS84	BC29706	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29706_Aamu_Bax2006_COI	NEWA43	1	BC29706_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29706	Sample	BC29706	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA43	COI	45	BC29706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA43	GCACAACCGGGATCTTTACTTC	151.75584
BC29706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88377	WGS84	BC29706	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29706_Pcan_Bot2011_IGS	NEWA43	1	BC29706_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29706	Sample	BC29706	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA43	IGS	45	BC29706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA43	CGTAATCCTCAGTACTGGCTA	151.75584
BC29706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88377	WGS84	BC29706	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29706_Sspa_Oph2007_COI	NEWA43	1	BC29706_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29706	Sample	BC29706	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA43	COI	45	BC29706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA43	GAAGGCCTTCCGATGATC	151.75584
BC29706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88377	WGS84	BC29706	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29706_Upin_Bot2011_COI	NEWA43	1	BC29706_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29706	Sample	BC29706	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA43	COI	45	BC29706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA43	CTTTAATTACAGCGTTTTTATTGTTGT	151.75584
BC29707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88398	WGS84	BC29707	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.826	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29707_Mgig_Bot2012_COI	NEWA42	1	BC29707_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29707	Sample	BC29707	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA42	COI	45	BC29707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA42	TCTTATTCGTTGGAGACTTTATAACCCT	151.75356
BC29708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88557	WGS84	BC29708	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29708_Cmae_Bot2011_COI	NEWA41	1	BC29708_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29708	Sample	BC29708	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA41	COI	45	BC29708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA41	ATGAACAGTCTATCCTCCTTTAG	151.75728
BC29708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88557	WGS84	BC29708	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29708_Msal_Bot2012_COI	NEWA41	1	BC29708_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29708	Sample	BC29708	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA41	COI	45	BC29708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA41	GYTAGTTCCRATGATGTTAGCTG	151.75728
BC29710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88718	WGS84	BC29710	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.495	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29710_Mgig_Bot2012_COI	NEWA39	1	BC29710_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29710	Sample	BC29710	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA39	COI	45	BC29710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA39	TCTTATTCGTTGGAGACTTTATAACCCT	151.76167
BC29711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88679	WGS84	BC29711	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29711_Cmae_Bot2011_COI	NEWA38	1	BC29711_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29711	Sample	BC29711	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA38	COI	45	BC29711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA38	ATGAACAGTCTATCCTCCTTTAG	151.75868
BC29711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88679	WGS84	BC29711	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29711_Msal_Bot2012_COI	NEWA38	1	BC29711_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29711	Sample	BC29711	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA38	COI	45	BC29711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA38	GYTAGTTCCRATGATGTTAGCTG	151.75868
BC29713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8887	WGS84	BC29713	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29713_Aamu_Bax2006_COI	NEWA36	1	BC29713_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29713	Sample	BC29713	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA36	COI	45	BC29713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA36	GCACAACCGGGATCTTTACTTC	151.76318
BC29713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8887	WGS84	BC29713	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29713_Pcan_Bot2011_IGS	NEWA36	1	BC29713_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29713	Sample	BC29713	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA36	IGS	45	BC29713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA36	CGTAATCCTCAGTACTGGCTA	151.76318
BC29713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8887	WGS84	BC29713	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29713_Sspa_Oph2007_COI	NEWA36	1	BC29713_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29713	Sample	BC29713	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA36	COI	45	BC29713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA36	GAAGGCCTTCCGATGATC	151.76318
BC29713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8887	WGS84	BC29713	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29713_Upin_Bot2011_COI	NEWA36	1	BC29713_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29713	Sample	BC29713	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA36	COI	45	BC29713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA36	CTTTAATTACAGCGTTTTTATTGTTGT	151.76318
BC29714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88836	WGS84	BC29714	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.683	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29714_Mgig_Bot2012_COI	NEWA35	1	BC29714_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29714	Sample	BC29714	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA35	COI	45	BC29714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA35	TCTTATTCGTTGGAGACTTTATAACCCT	151.76012
BC29715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88952	WGS84	BC29715	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29715_Cmae_Bot2011_COI	NEWA34	1	BC29715_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29715	Sample	BC29715	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA34	COI	45	BC29715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA34	ATGAACAGTCTATCCTCCTTTAG	151.76945
BC29715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88952	WGS84	BC29715	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29715_Msal_Bot2012_COI	NEWA34	1	BC29715_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29715	Sample	BC29715	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA34	COI	45	BC29715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA34	GYTAGTTCCRATGATGTTAGCTG	151.76945
BC29717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8901	WGS84	BC29717	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29717_Aamu_Bax2006_COI	NEWA32	1	BC29717_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29717	Sample	BC29717	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA32	COI	45	BC29717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA32	GCACAACCGGGATCTTTACTTC	151.76482
BC29717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8901	WGS84	BC29717	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29717_Pcan_Bot2011_IGS	NEWA32	1	BC29717_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29717	Sample	BC29717	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA32	IGS	45	BC29717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA32	CGTAATCCTCAGTACTGGCTA	151.76482
BC29717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8901	WGS84	BC29717	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29717_Sspa_Oph2007_COI	NEWA32	1	BC29717_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29717	Sample	BC29717	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA32	COI	45	BC29717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA32	GAAGGCCTTCCGATGATC	151.76482
BC29717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8901	WGS84	BC29717	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29717_Upin_Bot2011_COI	NEWA32	1	BC29717_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29717	Sample	BC29717	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA32	COI	45	BC29717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA32	CTTTAATTACAGCGTTTTTATTGTTGT	151.76482
BC29718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89009	WGS84	BC29718	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.376	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29718_Mgig_Bot2012_COI	NEWA31	1	BC29718_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29718	Sample	BC29718	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA31	COI	45	BC29718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA31	TCTTATTCGTTGGAGACTTTATAACCCT	151.77235
BC29719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89109	WGS84	BC29719	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29719_Cmae_Bot2011_COI	NEWA30	1	BC29719_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29719	Sample	BC29719	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA30	COI	45	BC29719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA30	ATGAACAGTCTATCCTCCTTTAG	151.77088
BC29719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89109	WGS84	BC29719	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29719_Msal_Bot2012_COI	NEWA30	1	BC29719_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29719	Sample	BC29719	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA30	COI	45	BC29719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA30	GYTAGTTCCRATGATGTTAGCTG	151.77088
BC29720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89121	WGS84	BC29720	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29720_Aamu_Bax2006_COI	NEWA29	1	BC29720_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29720	Sample	BC29720	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA29	COI	45	BC29720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA29	GCACAACCGGGATCTTTACTTC	151.76873
BC29720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89121	WGS84	BC29720	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29720_Pcan_Bot2011_IGS	NEWA29	1	BC29720_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29720	Sample	BC29720	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA29	IGS	45	BC29720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA29	CGTAATCCTCAGTACTGGCTA	151.76873
BC29720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89121	WGS84	BC29720	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29720_Sspa_Oph2007_COI	NEWA29	1	BC29720_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29720	Sample	BC29720	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA29	COI	45	BC29720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA29	GAAGGCCTTCCGATGATC	151.76873
BC29720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89121	WGS84	BC29720	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29720_Upin_Bot2011_COI	NEWA29	1	BC29720_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29720	Sample	BC29720	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA29	COI	45	BC29720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA29	CTTTAATTACAGCGTTTTTATTGTTGT	151.76873
BC29721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89171	WGS84	BC29721	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.026	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29721_Mgig_Bot2012_COI	NEWA28	1	BC29721_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29721	Sample	BC29721	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA28	COI	45	BC29721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA28	TCTTATTCGTTGGAGACTTTATAACCCT	151.76605
BC29722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89277	WGS84	BC29722	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29722_Cmae_Bot2011_COI	NEWA27	1	BC29722_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29722	Sample	BC29722	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA27	COI	45	BC29722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA27	ATGAACAGTCTATCCTCCTTTAG	151.77197
BC29722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89277	WGS84	BC29722	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29722_Msal_Bot2012_COI	NEWA27	1	BC29722_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29722	Sample	BC29722	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA27	COI	45	BC29722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA27	GYTAGTTCCRATGATGTTAGCTG	151.77197
BC29724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89331	WGS84	BC29724	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29724_Aamu_Bax2006_COI	NEWA25	1	BC29724_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29724	Sample	BC29724	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA25	COI	45	BC29724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA25	GCACAACCGGGATCTTTACTTC	151.76761
BC29724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89331	WGS84	BC29724	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29724_Pcan_Bot2011_IGS	NEWA25	1	BC29724_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29724	Sample	BC29724	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA25	IGS	45	BC29724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA25	CGTAATCCTCAGTACTGGCTA	151.76761
BC29724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89331	WGS84	BC29724	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29724_Sspa_Oph2007_COI	NEWA25	1	BC29724_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29724	Sample	BC29724	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA25	COI	45	BC29724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA25	GAAGGCCTTCCGATGATC	151.76761
BC29724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89331	WGS84	BC29724	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29724_Upin_Bot2011_COI	NEWA25	1	BC29724_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29724	Sample	BC29724	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA25	COI	45	BC29724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA25	CTTTAATTACAGCGTTTTTATTGTTGT	151.76761
BC29725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89442	WGS84	BC29725	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.551	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29725_Mgig_Bot2012_COI	NEWA24	1	BC29725_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29725	Sample	BC29725	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA24	COI	45	BC29725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA24	TCTTATTCGTTGGAGACTTTATAACCCT	151.77146
BC29726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89485	WGS84	BC29726	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29726_Cmae_Bot2011_COI	NEWA23	1	BC29726_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29726	Sample	BC29726	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA23	COI	45	BC29726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA23	ATGAACAGTCTATCCTCCTTTAG	151.76897
BC29726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89485	WGS84	BC29726	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29726_Msal_Bot2012_COI	NEWA23	1	BC29726_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29726	Sample	BC29726	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA23	COI	45	BC29726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA23	GYTAGTTCCRATGATGTTAGCTG	151.76897
BC29728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89642	WGS84	BC29728	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29728_Aamu_Bax2006_COI	NEWA21	1	BC29728_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29728	Sample	BC29728	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA21	COI	45	BC29728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA21	GCACAACCGGGATCTTTACTTC	151.7701
BC29728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89642	WGS84	BC29728	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29728_Pcan_Bot2011_IGS	NEWA21	1	BC29728_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29728	Sample	BC29728	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA21	IGS	45	BC29728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA21	CGTAATCCTCAGTACTGGCTA	151.7701
BC29728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89642	WGS84	BC29728	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29728_Sspa_Oph2007_COI	NEWA21	1	BC29728_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29728	Sample	BC29728	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA21	COI	45	BC29728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA21	GAAGGCCTTCCGATGATC	151.7701
BC29728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89642	WGS84	BC29728	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29728_Upin_Bot2011_COI	NEWA21	1	BC29728_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29728	Sample	BC29728	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA21	COI	45	BC29728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA21	CTTTAATTACAGCGTTTTTATTGTTGT	151.7701
BC29729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91159	WGS84	BC29729	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.246	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29729_Mgig_Bot2012_COI	NEWA20	1	BC29729_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29729	Sample	BC29729	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA20	COI	45	BC29729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA20	TCTTATTCGTTGGAGACTTTATAACCCT	151.77401
BC29731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91522	WGS84	BC29731	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29731_Aamu_Bax2006_COI	NEWA18	1	BC29731_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29731	Sample	BC29731	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA18	COI	45	BC29731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA18	GCACAACCGGGATCTTTACTTC	151.77531
BC29731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91522	WGS84	BC29731	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29731_Pcan_Bot2011_IGS	NEWA18	1	BC29731_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29731	Sample	BC29731	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA18	IGS	45	BC29731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA18	CGTAATCCTCAGTACTGGCTA	151.77531
BC29731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91522	WGS84	BC29731	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29731_Sspa_Oph2007_COI	NEWA18	1	BC29731_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29731	Sample	BC29731	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA18	COI	45	BC29731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA18	GAAGGCCTTCCGATGATC	151.77531
BC29731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91522	WGS84	BC29731	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29731_Upin_Bot2011_COI	NEWA18	1	BC29731_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29731	Sample	BC29731	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA18	COI	45	BC29731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA18	CTTTAATTACAGCGTTTTTATTGTTGT	151.77531
BC29732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91678	WGS84	BC29732	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.089	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29732_Mgig_Bot2012_COI	NEWA17	1	BC29732_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29732	Sample	BC29732	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA17	COI	45	BC29732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA17	TCTTATTCGTTGGAGACTTTATAACCCT	151.77668
BC29733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91741	WGS84	BC29733	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29733_Cmae_Bot2011_COI	NEWA16	1	BC29733_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29733	Sample	BC29733	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA16	COI	45	BC29733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA16	ATGAACAGTCTATCCTCCTTTAG	151.774
BC29733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91741	WGS84	BC29733	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29733_Msal_Bot2012_COI	NEWA16	1	BC29733_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29733	Sample	BC29733	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA16	COI	45	BC29733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA16	GYTAGTTCCRATGATGTTAGCTG	151.774
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29735_Aamu_Bax2006_COI	NEWA14	1	BC29735_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	GCACAACCGGGATCTTTACTTC	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29735_Pcan_Bot2011_IGS	NEWA14	1	BC29735_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA14	IGS	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	CGTAATCCTCAGTACTGGCTA	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29735_Pper_Dia2013_COI	NEWA14	1	BC29735_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	CTTAGTGGCATTAATTCGDAATCC	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29735_Sspa_Oph2007_COI	NEWA14	1	BC29735_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	GAAGGCCTTCCGATGATC	151.777
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29736_Cjap_Sim2018_COI	NEWA13	1	BC29736_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29736_Esin_And2018_Cyb	NEWA13	1	BC29736_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA13	Cytb	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	ACCCCTCCTCATATCCAACCA	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.874	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29736_Mgig_Bot2012_COI	NEWA13	1	BC29736_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	TCTTATTCGTTGGAGACTTTATAACCCT	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29736_Mjap_Gib2022_COI	NEWA13	1	BC29736_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	AATTTAATTGTGACTAGGCATGGTCTG	151.77052
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29737_Cmae_Bot2011_COI	NEWA12	1	BC29737_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	ATGAACAGTCTATCCTCCTTTAG	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29737_Hsan_Wil2021_COI	NEWA12	1	BC29737_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	CCTGGGCCGGTATAGTAGGT	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29737_Mros_Gib2022_COI	NEWA12	1	BC29737_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29737_Msal_Bot2012_COI	NEWA12	1	BC29737_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	GYTAGTTCCRATGATGTTAGCTG	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29737_Pvir_Dia2013_COI	NEWA12	1	BC29737_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	CTTAGTGGCATTAATTCGDAATCC	151.77158
BC29737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90124	WGS84	BC29737	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29737_Rhar_Sim2018_COI	NEWA12	1	BC29737_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29737	Sample	BC29737	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA12	COI	45	BC29737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA12	CTGCTGCTATTGCTCATG	151.77158
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29739_Aamu_Bax2006_COI	NEWA10	1	BC29739_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	GCACAACCGGGATCTTTACTTC	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29739_Pcan_Bot2011_IGS	NEWA10	1	BC29739_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA10	IGS	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	CGTAATCCTCAGTACTGGCTA	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29739_Pper_Dia2013_COI	NEWA10	1	BC29739_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	CTTAGTGGCATTAATTCGDAATCC	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29739_Sspa_Oph2007_COI	NEWA10	1	BC29739_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	GAAGGCCTTCCGATGATC	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29739_Upin_Bot2011_COI	NEWA10	1	BC29739_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	CTTTAATTACAGCGTTTTTATTGTTGT	151.77133
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29740_Cmae_Bot2011_COI	NEWA9	1	BC29740_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	ATGAACAGTCTATCCTCCTTTAG	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29740_Hsan_Wil2021_COI	NEWA9	1	BC29740_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	CCTGGGCCGGTATAGTAGGT	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29740_Mros_Gib2022_COI	NEWA9	1	BC29740_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29740_Msal_Bot2012_COI	NEWA9	1	BC29740_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	GYTAGTTCCRATGATGTTAGCTG	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29740_Pvir_Dia2013_COI	NEWA9	1	BC29740_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	CTTAGTGGCATTAATTCGDAATCC	151.7724
BC29740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90457	WGS84	BC29740	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29740_Rhar_Sim2018_COI	NEWA9	1	BC29740_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29740	Sample	BC29740	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA9	COI	45	BC29740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA9	CTGCTGCTATTGCTCATG	151.7724
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29742_Aamu_Bax2006_COI	NEWA7	1	BC29742_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	GCACAACCGGGATCTTTACTTC	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29742_Pcan_Bot2011_IGS	NEWA7	1	BC29742_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA7	IGS	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	CGTAATCCTCAGTACTGGCTA	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29742_Pper_Dia2013_COI	NEWA7	1	BC29742_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	CTTAGTGGCATTAATTCGDAATCC	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29742_Sspa_Oph2007_COI	NEWA7	1	BC29742_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	GAAGGCCTTCCGATGATC	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29742_Upin_Bot2011_COI	NEWA7	1	BC29742_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	CTTTAATTACAGCGTTTTTATTGTTGT	151.76974
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29743_Cjap_Sim2018_COI	NEWA6	1	BC29743_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29743_Esin_And2018_Cyb	NEWA6	1	BC29743_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA6	Cytb	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	ACCCCTCCTCATATCCAACCA	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.389	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29743_Mgig_Bot2012_COI	NEWA6	1	BC29743_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	TCTTATTCGTTGGAGACTTTATAACCCT	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29743_Mjap_Gib2022_COI	NEWA6	1	BC29743_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	AATTTAATTGTGACTAGGCATGGTCTG	151.77058
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29744_Cmae_Bot2011_COI	NEWA5	1	BC29744_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	ATGAACAGTCTATCCTCCTTTAG	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29744_Hsan_Wil2021_COI	NEWA5	1	BC29744_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	CCTGGGCCGGTATAGTAGGT	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29744_Mros_Gib2022_COI	NEWA5	1	BC29744_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29744_Msal_Bot2012_COI	NEWA5	1	BC29744_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	GYTAGTTCCRATGATGTTAGCTG	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29744_Pvir_Dia2013_COI	NEWA5	1	BC29744_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	CTTAGTGGCATTAATTCGDAATCC	151.76814
BC29744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92253	WGS84	BC29744	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29744_Rhar_Sim2018_COI	NEWA5	1	BC29744_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29744	Sample	BC29744	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA5	COI	45	BC29744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA5	CTGCTGCTATTGCTCATG	151.76814
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29746_Aamu_Bax2006_COI	NEWA3	1	BC29746_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	GCACAACCGGGATCTTTACTTC	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29746_Pcan_Bot2011_IGS	NEWA3	1	BC29746_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA3	IGS	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	CGTAATCCTCAGTACTGGCTA	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29746_Pper_Dia2013_COI	NEWA3	1	BC29746_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	CTTAGTGGCATTAATTCGDAATCC	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29746_Sspa_Oph2007_COI	NEWA3	1	BC29746_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	GAAGGCCTTCCGATGATC	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29746_Upin_Bot2011_COI	NEWA3	1	BC29746_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	CTTTAATTACAGCGTTTTTATTGTTGT	151.77067
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29747_Cjap_Sim2018_COI	NEWA2	1	BC29747_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29747_Esin_And2018_Cyb	NEWA2	1	BC29747_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA2	Cytb	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	ACCCCTCCTCATATCCAACCA	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.979	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29747_Mgig_Bot2012_COI	NEWA2	1	BC29747_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	TCTTATTCGTTGGAGACTTTATAACCCT	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29747_Mjap_Gib2022_COI	NEWA2	1	BC29747_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	AATTTAATTGTGACTAGGCATGGTCTG	151.76782
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29748_Cmae_Bot2011_COI	NEWA1	1	BC29748_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	ATGAACAGTCTATCCTCCTTTAG	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29748_Hsan_Wil2021_COI	NEWA1	1	BC29748_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	CCTGGGCCGGTATAGTAGGT	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29748_Mros_Gib2022_COI	NEWA1	1	BC29748_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29748_Msal_Bot2012_COI	NEWA1	1	BC29748_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	GYTAGTTCCRATGATGTTAGCTG	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29748_Pvir_Dia2013_COI	NEWA1	1	BC29748_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	CTTAGTGGCATTAATTCGDAATCC	151.76515
BC29748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92464	WGS84	BC29748	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29748_Rhar_Sim2018_COI	NEWA1	1	BC29748_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29748	Sample	BC29748	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA1	COI	45	BC29748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA1	CTGCTGCTATTGCTCATG	151.76515
BC29750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45408	WGS84	BC29750	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.388	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29750_Mgig_Bot2012_COI	KEMA34	1	BC29750_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29750	Sample	BC29750	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA34	COI	45	BC29750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA34	TCTTATTCGTTGGAGACTTTATAACCCT	150.89427
BC29751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45615	WGS84	BC29751	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29751_Cmae_Bot2011_COI	KEMA33	1	BC29751_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29751	Sample	BC29751	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA33	COI	45	BC29751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA33	ATGAACAGTCTATCCTCCTTTAG	150.88729
BC29751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45615	WGS84	BC29751	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29751_Msal_Bot2012_COI	KEMA33	1	BC29751_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29751	Sample	BC29751	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA33	COI	45	BC29751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA33	GYTAGTTCCRATGATGTTAGCTG	150.88729
BC29753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45621	WGS84	BC29753	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29753_Aamu_Bax2006_COI	KEMA31	1	BC29753_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29753	Sample	BC29753	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA31	COI	45	BC29753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA31	GCACAACCGGGATCTTTACTTC	150.89105
BC29753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45621	WGS84	BC29753	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29753_Pcan_Bot2011_IGS	KEMA31	1	BC29753_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29753	Sample	BC29753	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA31	IGS	45	BC29753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA31	CGTAATCCTCAGTACTGGCTA	150.89105
BC29753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45621	WGS84	BC29753	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29753_Sspa_Oph2007_COI	KEMA31	1	BC29753_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29753	Sample	BC29753	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA31	COI	45	BC29753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA31	GAAGGCCTTCCGATGATC	150.89105
BC29753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45621	WGS84	BC29753	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29753_Upin_Bot2011_COI	KEMA31	1	BC29753_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29753	Sample	BC29753	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA31	COI	45	BC29753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA31	CTTTAATTACAGCGTTTTTATTGTTGT	150.89105
BC29754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45643	WGS84	BC29754	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.168	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29754_Mgig_Bot2012_COI	KEMA30	1	BC29754_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29754	Sample	BC29754	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA30	COI	45	BC29754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA30	TCTTATTCGTTGGAGACTTTATAACCCT	150.88892
BC29755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45745	WGS84	BC29755	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29755_Cmae_Bot2011_COI	KEMA29	1	BC29755_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29755	Sample	BC29755	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA29	COI	45	BC29755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA29	ATGAACAGTCTATCCTCCTTTAG	150.89471
BC29755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45745	WGS84	BC29755	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29755_Msal_Bot2012_COI	KEMA29	1	BC29755_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29755	Sample	BC29755	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA29	COI	45	BC29755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA29	GYTAGTTCCRATGATGTTAGCTG	150.89471
BC29757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45868	WGS84	BC29757	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29757_Aamu_Bax2006_COI	KEMA27	1	BC29757_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29757	Sample	BC29757	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA27	COI	45	BC29757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA27	GCACAACCGGGATCTTTACTTC	150.89076
BC29757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45868	WGS84	BC29757	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29757_Pcan_Bot2011_IGS	KEMA27	1	BC29757_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29757	Sample	BC29757	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA27	IGS	45	BC29757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA27	CGTAATCCTCAGTACTGGCTA	150.89076
BC29757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45868	WGS84	BC29757	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29757_Sspa_Oph2007_COI	KEMA27	1	BC29757_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29757	Sample	BC29757	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA27	COI	45	BC29757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA27	GAAGGCCTTCCGATGATC	150.89076
BC29757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45868	WGS84	BC29757	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29757_Upin_Bot2011_COI	KEMA27	1	BC29757_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29757	Sample	BC29757	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA27	COI	45	BC29757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA27	CTTTAATTACAGCGTTTTTATTGTTGT	150.89076
BC29758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45976	WGS84	BC29758	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.232	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29758_Mgig_Bot2012_COI	KEMA26	1	BC29758_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29758	Sample	BC29758	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA26	COI	45	BC29758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA26	TCTTATTCGTTGGAGACTTTATAACCCT	150.89679
BC29759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45991	WGS84	BC29759	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29759_Cmae_Bot2011_COI	KEMA25	1	BC29759_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29759	Sample	BC29759	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA25	COI	45	BC29759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA25	ATGAACAGTCTATCCTCCTTTAG	150.89441
BC29759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45991	WGS84	BC29759	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29759_Msal_Bot2012_COI	KEMA25	1	BC29759_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29759	Sample	BC29759	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA25	COI	45	BC29759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA25	GYTAGTTCCRATGATGTTAGCTG	150.89441
BC29760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45994	WGS84	BC29760	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29760_Aamu_Bax2006_COI	KEMA24	1	BC29760_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29760	Sample	BC29760	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA24	COI	45	BC29760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA24	GCACAACCGGGATCTTTACTTC	150.8924
BC29760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45994	WGS84	BC29760	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29760_Pcan_Bot2011_IGS	KEMA24	1	BC29760_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29760	Sample	BC29760	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA24	IGS	45	BC29760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA24	CGTAATCCTCAGTACTGGCTA	150.8924
BC29760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45994	WGS84	BC29760	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29760_Sspa_Oph2007_COI	KEMA24	1	BC29760_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29760	Sample	BC29760	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA24	COI	45	BC29760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA24	GAAGGCCTTCCGATGATC	150.8924
BC29760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45994	WGS84	BC29760	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29760_Upin_Bot2011_COI	KEMA24	1	BC29760_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29760	Sample	BC29760	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA24	COI	45	BC29760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA24	CTTTAATTACAGCGTTTTTATTGTTGT	150.8924
BC29761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4613	WGS84	BC29761	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.467	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29761_Mgig_Bot2012_COI	KEMA23	1	BC29761_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29761	Sample	BC29761	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA23	COI	45	BC29761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA23	TCTTATTCGTTGGAGACTTTATAACCCT	150.89796
BC29762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46148	WGS84	BC29762	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29762_Cmae_Bot2011_COI	KEMA22	1	BC29762_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29762	Sample	BC29762	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA22	COI	45	BC29762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA22	ATGAACAGTCTATCCTCCTTTAG	150.89598
BC29762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46148	WGS84	BC29762	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29762_Msal_Bot2012_COI	KEMA22	1	BC29762_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29762	Sample	BC29762	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA22	COI	45	BC29762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA22	GYTAGTTCCRATGATGTTAGCTG	150.89598
BC29764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46245	WGS84	BC29764	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29764_Aamu_Bax2006_COI	KEMA20	1	BC29764_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29764	Sample	BC29764	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA20	COI	45	BC29764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA20	GCACAACCGGGATCTTTACTTC	150.89685
BC29764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46245	WGS84	BC29764	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29764_Pcan_Bot2011_IGS	KEMA20	1	BC29764_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29764	Sample	BC29764	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA20	IGS	45	BC29764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA20	CGTAATCCTCAGTACTGGCTA	150.89685
BC29764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46245	WGS84	BC29764	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29764_Sspa_Oph2007_COI	KEMA20	1	BC29764_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29764	Sample	BC29764	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA20	COI	45	BC29764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA20	GAAGGCCTTCCGATGATC	150.89685
BC29764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46245	WGS84	BC29764	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29764_Upin_Bot2011_COI	KEMA20	1	BC29764_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29764	Sample	BC29764	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA20	COI	45	BC29764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA20	CTTTAATTACAGCGTTTTTATTGTTGT	150.89685
BC29765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46331	WGS84	BC29765	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.631	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29765_Mgig_Bot2012_COI	KEMA19	1	BC29765_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29765	Sample	BC29765	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA19	COI	45	BC29765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA19	TCTTATTCGTTGGAGACTTTATAACCCT	150.90459
BC29766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46396	WGS84	BC29766	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29766_Cmae_Bot2011_COI	KEMA18	1	BC29766_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29766	Sample	BC29766	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA18	COI	45	BC29766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA18	ATGAACAGTCTATCCTCCTTTAG	150.90323
BC29766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46396	WGS84	BC29766	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29766_Msal_Bot2012_COI	KEMA18	1	BC29766_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29766	Sample	BC29766	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA18	COI	45	BC29766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA18	GYTAGTTCCRATGATGTTAGCTG	150.90323
BC29768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46436	WGS84	BC29768	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29768_Aamu_Bax2006_COI	KEMA16	1	BC29768_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29768	Sample	BC29768	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA16	COI	45	BC29768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA16	GCACAACCGGGATCTTTACTTC	150.90839
BC29768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46436	WGS84	BC29768	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29768_Pcan_Bot2011_IGS	KEMA16	1	BC29768_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29768	Sample	BC29768	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA16	IGS	45	BC29768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA16	CGTAATCCTCAGTACTGGCTA	150.90839
BC29768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46436	WGS84	BC29768	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29768_Sspa_Oph2007_COI	KEMA16	1	BC29768_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29768	Sample	BC29768	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA16	COI	45	BC29768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA16	GAAGGCCTTCCGATGATC	150.90839
BC29768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46436	WGS84	BC29768	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29768_Upin_Bot2011_COI	KEMA16	1	BC29768_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29768	Sample	BC29768	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA16	COI	45	BC29768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA16	CTTTAATTACAGCGTTTTTATTGTTGT	150.90839
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46501	WGS84	BC29769	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29769_Asen_Wil2025_COI	KEMA15	1	BC29769_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA15	COI	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	GTTGTTGTTACAACACATGCTCTTTTG	150.90702
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46501	WGS84	BC29769	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.325	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29769_Mgig_Bot2012_COI	KEMA15	1	BC29769_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA15	COI	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	TCTTATTCGTTGGAGACTTTATAACCCT	150.90702
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46606	WGS84	BC29771	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29771_Aamu_Bax2006_COI	KEMA12	1	BC29771_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	GCACAACCGGGATCTTTACTTC	150.90836
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46606	WGS84	BC29771	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29771_Pcan_Bot2011_IGS	KEMA12	1	BC29771_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA12	IGS	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	CGTAATCCTCAGTACTGGCTA	150.90836
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46606	WGS84	BC29771	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29771_Sspa_Oph2007_COI	KEMA12	1	BC29771_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	GAAGGCCTTCCGATGATC	150.90836
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46606	WGS84	BC29771	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29771_Upin_Bot2011_COI	KEMA12	1	BC29771_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	CTTTAATTACAGCGTTTTTATTGTTGT	150.90836
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46606	WGS84	BC29771	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29771_Vgib_Wil2025_COI	KEMA12	1	BC29771_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	CCCCTCTTTACAAGTTCTTTAGGGT	150.90836
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46705	WGS84	BC29772	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29772_Asen_Wil2025_COI	KEMA11	1	BC29772_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	GTTGTTGTTACAACACATGCTCTTTTG	150.90599
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46705	WGS84	BC29772	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.646	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29772_Mgig_Bot2012_COI	KEMA11	1	BC29772_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	TCTTATTCGTTGGAGACTTTATAACCCT	150.90599
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46723	WGS84	BC29773	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29773_Cmae_Bot2011_COI	KEMA10	1	BC29773_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	ATGAACAGTCTATCCTCCTTTAG	150.90388
BC29773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46723	WGS84	BC29773	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29773_Msal_Bot2012_COI	KEMA10	1	BC29773_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29773	Sample	BC29773	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA10	COI	45	BC29773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA10	GYTAGTTCCRATGATGTTAGCTG	150.90388
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46673	WGS84	BC29775	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29775_Aamu_Bax2006_COI	KEMA9	1	BC29775_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	GCACAACCGGGATCTTTACTTC	150.90128
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46673	WGS84	BC29775	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29775_Pcan_Bot2011_IGS	KEMA9	1	BC29775_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA9	IGS	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	CGTAATCCTCAGTACTGGCTA	150.90128
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46673	WGS84	BC29775	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29775_Sspa_Oph2007_COI	KEMA9	1	BC29775_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	GAAGGCCTTCCGATGATC	150.90128
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46673	WGS84	BC29775	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29775_Upin_Bot2011_COI	KEMA9	1	BC29775_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	CTTTAATTACAGCGTTTTTATTGTTGT	150.90128
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46673	WGS84	BC29775	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29775_Vgib_Wil2025_COI	KEMA9	1	BC29775_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	CCCCTCTTTACAAGTTCTTTAGGGT	150.90128
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46841	WGS84	BC29776	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29776_Asen_Wil2025_COI	KEMA8	1	BC29776_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	GTTGTTGTTACAACACATGCTCTTTTG	150.90758
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46841	WGS84	BC29776	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.7	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29776_Mgig_Bot2012_COI	KEMA8	1	BC29776_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	TCTTATTCGTTGGAGACTTTATAACCCT	150.90758
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46844	WGS84	BC29777	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29777_Cmae_Bot2011_COI	KEMA7	1	BC29777_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	ATGAACAGTCTATCCTCCTTTAG	150.90496
BC29777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46844	WGS84	BC29777	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29777_Msal_Bot2012_COI	KEMA7	1	BC29777_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29777	Sample	BC29777	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA7	COI	45	BC29777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA7	GYTAGTTCCRATGATGTTAGCTG	150.90496
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46879	WGS84	BC29779	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29779_Aamu_Bax2006_COI	KEMA5	1	BC29779_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	GCACAACCGGGATCTTTACTTC	150.91175
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46879	WGS84	BC29779	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29779_Pcan_Bot2011_IGS	KEMA5	1	BC29779_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA5	IGS	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	CGTAATCCTCAGTACTGGCTA	150.91175
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46879	WGS84	BC29779	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29779_Sspa_Oph2007_COI	KEMA5	1	BC29779_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	GAAGGCCTTCCGATGATC	150.91175
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46879	WGS84	BC29779	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29779_Upin_Bot2011_COI	KEMA5	1	BC29779_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	CTTTAATTACAGCGTTTTTATTGTTGT	150.91175
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46879	WGS84	BC29779	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29779_Vgib_Wil2025_COI	KEMA5	1	BC29779_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	CCCCTCTTTACAAGTTCTTTAGGGT	150.91175
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4697	WGS84	BC29780	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29780_Cmae_Bot2011_COI	KEMA4	1	BC29780_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	ATGAACAGTCTATCCTCCTTTAG	150.90884
BC29780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4697	WGS84	BC29780	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29780_Msal_Bot2012_COI	KEMA4	1	BC29780_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29780	Sample	BC29780	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA4	COI	45	BC29780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA4	GYTAGTTCCRATGATGTTAGCTG	150.90884
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47213	WGS84	BC29782	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29782_Aamu_Bax2006_COI	KEMA2	1	BC29782_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	GCACAACCGGGATCTTTACTTC	150.91224
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47213	WGS84	BC29782	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29782_Pcan_Bot2011_IGS	KEMA2	1	BC29782_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA2	IGS	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	CGTAATCCTCAGTACTGGCTA	150.91224
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47213	WGS84	BC29782	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29782_Sspa_Oph2007_COI	KEMA2	1	BC29782_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	GAAGGCCTTCCGATGATC	150.91224
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47213	WGS84	BC29782	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29782_Upin_Bot2011_COI	KEMA2	1	BC29782_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	CTTTAATTACAGCGTTTTTATTGTTGT	150.91224
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.47213	WGS84	BC29782	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29782_Vgib_Wil2025_COI	KEMA2	1	BC29782_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	CCCCTCTTTACAAGTTCTTTAGGGT	150.91224
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.47312	WGS84	BC29783	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29783_Asen_Wil2025_COI	KEMA1	1	BC29783_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	GTTGTTGTTACAACACATGCTCTTTTG	150.90939
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47312	WGS84	BC29783	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.032	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29783_Mgig_Bot2012_COI	KEMA1	1	BC29783_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	TCTTATTCGTTGGAGACTTTATAACCCT	150.90939
BC29784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96258	WGS84	BC29784	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29784_Cmae_Bot2011_COI	BOTA40	1	BC29784_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29784	Sample	BC29784	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA40	COI	45	BC29784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA40	ATGAACAGTCTATCCTCCTTTAG	151.19841
BC29784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96258	WGS84	BC29784	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29784_Msal_Bot2012_COI	BOTA40	1	BC29784_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29784	Sample	BC29784	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA40	COI	45	BC29784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA40	GYTAGTTCCRATGATGTTAGCTG	151.19841
BC29786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96074	WGS84	BC29786	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29786_Aamu_Bax2006_COI	BOTA41	1	BC29786_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29786	Sample	BC29786	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA41	COI	45	BC29786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA41	GCACAACCGGGATCTTTACTTC	151.19651
BC29786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96074	WGS84	BC29786	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29786_Pcan_Bot2011_IGS	BOTA41	1	BC29786_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29786	Sample	BC29786	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA41	IGS	45	BC29786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA41	CGTAATCCTCAGTACTGGCTA	151.19651
BC29786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96074	WGS84	BC29786	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29786_Sspa_Oph2007_COI	BOTA41	1	BC29786_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29786	Sample	BC29786	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA41	COI	45	BC29786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA41	GAAGGCCTTCCGATGATC	151.19651
BC29786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96074	WGS84	BC29786	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29786_Upin_Bot2011_COI	BOTA41	1	BC29786_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29786	Sample	BC29786	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA41	COI	45	BC29786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA41	CTTTAATTACAGCGTTTTTATTGTTGT	151.19651
BC29787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96424	WGS84	BC29787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.137	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29787_Mgig_Bot2012_COI	BOTA39	1	BC29787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29787	Sample	BC29787	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA39	COI	45	BC29787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA39	TCTTATTCGTTGGAGACTTTATAACCCT	151.19718
BC29788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96557	WGS84	BC29788	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29788_Cmae_Bot2011_COI	BOTA38	1	BC29788_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29788	Sample	BC29788	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA38	COI	45	BC29788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA38	ATGAACAGTCTATCCTCCTTTAG	151.19871
BC29788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96557	WGS84	BC29788	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29788_Msal_Bot2012_COI	BOTA38	1	BC29788_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29788	Sample	BC29788	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA38	COI	45	BC29788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA38	GYTAGTTCCRATGATGTTAGCTG	151.19871
BC29790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96914	WGS84	BC29790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.921	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29790_Mgig_Bot2012_COI	BOTA36	1	BC29790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29790	Sample	BC29790	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA36	COI	45	BC29790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA36	TCTTATTCGTTGGAGACTTTATAACCCT	151.19959
BC29791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9685	WGS84	BC29791	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29791_Cmae_Bot2011_COI	BOTA35	1	BC29791_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29791	Sample	BC29791	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA35	COI	45	BC29791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA35	ATGAACAGTCTATCCTCCTTTAG	151.2182
BC29791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9685	WGS84	BC29791	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29791_Msal_Bot2012_COI	BOTA35	1	BC29791_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29791	Sample	BC29791	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA35	COI	45	BC29791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA35	GYTAGTTCCRATGATGTTAGCTG	151.2182
BC29793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96896	WGS84	BC29793	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29793_Aamu_Bax2006_COI	BOTA33	1	BC29793_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29793	Sample	BC29793	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA33	COI	45	BC29793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA33	GCACAACCGGGATCTTTACTTC	151.214
BC29793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96896	WGS84	BC29793	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29793_Pcan_Bot2011_IGS	BOTA33	1	BC29793_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29793	Sample	BC29793	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA33	IGS	45	BC29793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA33	CGTAATCCTCAGTACTGGCTA	151.214
BC29793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96896	WGS84	BC29793	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29793_Sspa_Oph2007_COI	BOTA33	1	BC29793_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29793	Sample	BC29793	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA33	COI	45	BC29793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA33	GAAGGCCTTCCGATGATC	151.214
BC29793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96896	WGS84	BC29793	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29793_Upin_Bot2011_COI	BOTA33	1	BC29793_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29793	Sample	BC29793	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA33	COI	45	BC29793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA33	CTTTAATTACAGCGTTTTTATTGTTGT	151.214
BC29794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97029	WGS84	BC29794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.805	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29794_Mgig_Bot2012_COI	BOTA32	1	BC29794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29794	Sample	BC29794	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA32	COI	45	BC29794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA32	TCTTATTCGTTGGAGACTTTATAACCCT	151.20081
BC29795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97095	WGS84	BC29795	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29795_Cmae_Bot2011_COI	BOTA31	1	BC29795_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29795	Sample	BC29795	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA31	COI	45	BC29795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA31	ATGAACAGTCTATCCTCCTTTAG	151.19958
BC29795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97095	WGS84	BC29795	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29795_Msal_Bot2012_COI	BOTA31	1	BC29795_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29795	Sample	BC29795	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA31	COI	45	BC29795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA31	GYTAGTTCCRATGATGTTAGCTG	151.19958
BC29797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97063	WGS84	BC29797	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29797_Aamu_Bax2006_COI	BOTA29	1	BC29797_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29797	Sample	BC29797	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA29	COI	45	BC29797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA29	GCACAACCGGGATCTTTACTTC	151.21326
BC29797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97063	WGS84	BC29797	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29797_Pcan_Bot2011_IGS	BOTA29	1	BC29797_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29797	Sample	BC29797	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA29	IGS	45	BC29797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA29	CGTAATCCTCAGTACTGGCTA	151.21326
BC29797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97063	WGS84	BC29797	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29797_Sspa_Oph2007_COI	BOTA29	1	BC29797_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29797	Sample	BC29797	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA29	COI	45	BC29797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA29	GAAGGCCTTCCGATGATC	151.21326
BC29797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97063	WGS84	BC29797	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29797_Upin_Bot2011_COI	BOTA29	1	BC29797_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29797	Sample	BC29797	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA29	COI	45	BC29797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA29	CTTTAATTACAGCGTTTTTATTGTTGT	151.21326
BC29798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97089	WGS84	BC29798	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.089	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29798_Mgig_Bot2012_COI	BOTA28	1	BC29798_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29798	Sample	BC29798	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA28	COI	45	BC29798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA28	TCTTATTCGTTGGAGACTTTATAACCCT	151.21098
BC29799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9711	WGS84	BC29799	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29799_Cmae_Bot2011_COI	BOTA27	1	BC29799_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29799	Sample	BC29799	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA27	COI	45	BC29799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA27	ATGAACAGTCTATCCTCCTTTAG	151.20951
BC29799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9711	WGS84	BC29799	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29799_Msal_Bot2012_COI	BOTA27	1	BC29799_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29799	Sample	BC29799	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA27	COI	45	BC29799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA27	GYTAGTTCCRATGATGTTAGCTG	151.20951
BC29801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97164	WGS84	BC29801	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29801_Aamu_Bax2006_COI	BOTA25	1	BC29801_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29801	Sample	BC29801	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA25	COI	45	BC29801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA25	GCACAACCGGGATCTTTACTTC	151.2054
BC29801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97164	WGS84	BC29801	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29801_Pcan_Bot2011_IGS	BOTA25	1	BC29801_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29801	Sample	BC29801	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA25	IGS	45	BC29801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA25	CGTAATCCTCAGTACTGGCTA	151.2054
BC29801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97164	WGS84	BC29801	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29801_Sspa_Oph2007_COI	BOTA25	1	BC29801_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29801	Sample	BC29801	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA25	COI	45	BC29801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA25	GAAGGCCTTCCGATGATC	151.2054
BC29801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97164	WGS84	BC29801	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29801_Upin_Bot2011_COI	BOTA25	1	BC29801_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29801	Sample	BC29801	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA25	COI	45	BC29801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA25	CTTTAATTACAGCGTTTTTATTGTTGT	151.2054
BC29802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97198	WGS84	BC29802	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.937	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29802_Mgig_Bot2012_COI	BOTA24	1	BC29802_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29802	Sample	BC29802	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA24	COI	45	BC29802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA24	TCTTATTCGTTGGAGACTTTATAACCCT	151.2028
BC29803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97218	WGS84	BC29803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29803_Cmae_Bot2011_COI	BOTA23	1	BC29803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29803	Sample	BC29803	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA23	COI	45	BC29803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA23	ATGAACAGTCTATCCTCCTTTAG	151.20066
BC29803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97218	WGS84	BC29803	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29803_Msal_Bot2012_COI	BOTA23	1	BC29803_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29803	Sample	BC29803	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA23	COI	45	BC29803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA23	GYTAGTTCCRATGATGTTAGCTG	151.20066
BC29805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97277	WGS84	BC29805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29805_Aamu_Bax2006_COI	BOTA21	1	BC29805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29805	Sample	BC29805	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA21	COI	45	BC29805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA21	GCACAACCGGGATCTTTACTTC	151.20822
BC29805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97277	WGS84	BC29805	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29805_Pcan_Bot2011_IGS	BOTA21	1	BC29805_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29805	Sample	BC29805	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA21	IGS	45	BC29805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA21	CGTAATCCTCAGTACTGGCTA	151.20822
BC29805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97277	WGS84	BC29805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29805_Sspa_Oph2007_COI	BOTA21	1	BC29805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29805	Sample	BC29805	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA21	COI	45	BC29805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA21	GAAGGCCTTCCGATGATC	151.20822
BC29805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97277	WGS84	BC29805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29805_Upin_Bot2011_COI	BOTA21	1	BC29805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29805	Sample	BC29805	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA21	COI	45	BC29805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA21	CTTTAATTACAGCGTTTTTATTGTTGT	151.20822
BC29806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97333	WGS84	BC29806	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.334	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29806_Mgig_Bot2012_COI	BOTA20	1	BC29806_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29806	Sample	BC29806	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA20	COI	45	BC29806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA20	TCTTATTCGTTGGAGACTTTATAACCCT	151.20691
BC29807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9734	WGS84	BC29807	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29807_Cmae_Bot2011_COI	BOTA19	1	BC29807_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29807	Sample	BC29807	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA19	COI	45	BC29807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA19	ATGAACAGTCTATCCTCCTTTAG	151.20403
BC29807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9734	WGS84	BC29807	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29807_Msal_Bot2012_COI	BOTA19	1	BC29807_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29807	Sample	BC29807	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA19	COI	45	BC29807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA19	GYTAGTTCCRATGATGTTAGCTG	151.20403
BC29809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9739	WGS84	BC29809	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29809_Aamu_Bax2006_COI	BOTA17	1	BC29809_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29809	Sample	BC29809	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA17	COI	45	BC29809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA17	GCACAACCGGGATCTTTACTTC	151.20928
BC29809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9739	WGS84	BC29809	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29809_Pcan_Bot2011_IGS	BOTA17	1	BC29809_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29809	Sample	BC29809	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA17	IGS	45	BC29809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA17	CGTAATCCTCAGTACTGGCTA	151.20928
BC29809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9739	WGS84	BC29809	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29809_Sspa_Oph2007_COI	BOTA17	1	BC29809_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29809	Sample	BC29809	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA17	COI	45	BC29809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA17	GAAGGCCTTCCGATGATC	151.20928
BC29809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9739	WGS84	BC29809	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29809_Upin_Bot2011_COI	BOTA17	1	BC29809_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29809	Sample	BC29809	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA17	COI	45	BC29809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA17	CTTTAATTACAGCGTTTTTATTGTTGT	151.20928
BC29810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.974	WGS84	BC29810	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29810_Cmae_Bot2011_COI	BOTA16	1	BC29810_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29810	Sample	BC29810	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA16	COI	45	BC29810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA16	ATGAACAGTCTATCCTCCTTTAG	151.2075
BC29810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.974	WGS84	BC29810	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29810_Msal_Bot2012_COI	BOTA16	1	BC29810_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29810	Sample	BC29810	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA16	COI	45	BC29810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA16	GYTAGTTCCRATGATGTTAGCTG	151.2075
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29812_Aamu_Bax2006_COI	BOTA14	1	BC29812_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	GCACAACCGGGATCTTTACTTC	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29812_Pcan_Bot2011_IGS	BOTA14	1	BC29812_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA14	IGS	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	CGTAATCCTCAGTACTGGCTA	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29812_Pper_Dia2013_COI	BOTA14	1	BC29812_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	CTTAGTGGCATTAATTCGDAATCC	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29812_Sspa_Oph2007_COI	BOTA14	1	BC29812_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	GAAGGCCTTCCGATGATC	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29812_Upin_Bot2011_COI	BOTA14	1	BC29812_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	CTTTAATTACAGCGTTTTTATTGTTGT	151.20754
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29813_Cjap_Sim2018_COI	BOTA13	1	BC29813_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29813_Esin_And2018_Cyb	BOTA13	1	BC29813_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA13	Cytb	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	ACCCCTCCTCATATCCAACCA	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.351	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29813_Mgig_Bot2012_COI	BOTA13	1	BC29813_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	TCTTATTCGTTGGAGACTTTATAACCCT	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29813_Mjap_Gib2022_COI	BOTA13	1	BC29813_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	AATTTAATTGTGACTAGGCATGGTCTG	151.20882
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29814_Cmae_Bot2011_COI	BOTA12	1	BC29814_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	ATGAACAGTCTATCCTCCTTTAG	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29814_Hsan_Wil2021_COI	BOTA12	1	BC29814_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	CCTGGGCCGGTATAGTAGGT	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29814_Mros_Gib2022_COI	BOTA12	1	BC29814_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29814_Msal_Bot2012_COI	BOTA12	1	BC29814_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	GYTAGTTCCRATGATGTTAGCTG	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29814_Pvir_Dia2013_COI	BOTA12	1	BC29814_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	CTTAGTGGCATTAATTCGDAATCC	151.20642
BC29814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97885	WGS84	BC29814	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29814_Rhar_Sim2018_COI	BOTA12	1	BC29814_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29814	Sample	BC29814	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA12	COI	45	BC29814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA12	CTGCTGCTATTGCTCATG	151.20642
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29816_Aamu_Bax2006_COI	BOTA10	1	BC29816_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	GCACAACCGGGATCTTTACTTC	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29816_Pcan_Bot2011_IGS	BOTA10	1	BC29816_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA10	IGS	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	CGTAATCCTCAGTACTGGCTA	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29816_Pper_Dia2013_COI	BOTA10	1	BC29816_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	CTTAGTGGCATTAATTCGDAATCC	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.693	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29816_Sspa_Oph2007_COI	BOTA10	1	BC29816_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	GAAGGCCTTCCGATGATC	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29816_Upin_Bot2011_COI	BOTA10	1	BC29816_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	CTTTAATTACAGCGTTTTTATTGTTGT	151.20684
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29817_Cjap_Sim2018_COI	BOTA9	1	BC29817_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29817_Esin_And2018_Cyb	BOTA9	1	BC29817_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA9	Cytb	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	ACCCCTCCTCATATCCAACCA	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.452	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29817_Mgig_Bot2012_COI	BOTA9	1	BC29817_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	TCTTATTCGTTGGAGACTTTATAACCCT	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29817_Mjap_Gib2022_COI	BOTA9	1	BC29817_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	AATTTAATTGTGACTAGGCATGGTCTG	151.21203
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29818_Cmae_Bot2011_COI	BOTA8	1	BC29818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	ATGAACAGTCTATCCTCCTTTAG	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29818_Hsan_Wil2021_COI	BOTA8	1	BC29818_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	CCTGGGCCGGTATAGTAGGT	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29818_Mros_Gib2022_COI	BOTA8	1	BC29818_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29818_Msal_Bot2012_COI	BOTA8	1	BC29818_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	GYTAGTTCCRATGATGTTAGCTG	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29818_Pvir_Dia2013_COI	BOTA8	1	BC29818_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	CTTAGTGGCATTAATTCGDAATCC	151.20886
BC29818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99639	WGS84	BC29818	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29818_Rhar_Sim2018_COI	BOTA8	1	BC29818_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29818	Sample	BC29818	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA8	COI	45	BC29818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA8	CTGCTGCTATTGCTCATG	151.20886
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29820_Cjap_Sim2018_COI	BOTA6	1	BC29820_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29820_Esin_And2018_Cyb	BOTA6	1	BC29820_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA6	Cytb	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	ACCCCTCCTCATATCCAACCA	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.619	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29820_Mgig_Bot2012_COI	BOTA6	1	BC29820_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	TCTTATTCGTTGGAGACTTTATAACCCT	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29820_Mjap_Gib2022_COI	BOTA6	1	BC29820_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	AATTTAATTGTGACTAGGCATGGTCTG	151.20998
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29821_Cmae_Bot2011_COI	BOTA5	1	BC29821_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	ATGAACAGTCTATCCTCCTTTAG	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29821_Hsan_Wil2021_COI	BOTA5	1	BC29821_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	CCTGGGCCGGTATAGTAGGT	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29821_Mros_Gib2022_COI	BOTA5	1	BC29821_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29821_Msal_Bot2012_COI	BOTA5	1	BC29821_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	GYTAGTTCCRATGATGTTAGCTG	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29821_Pvir_Dia2013_COI	BOTA5	1	BC29821_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	CTTAGTGGCATTAATTCGDAATCC	151.20803
BC29821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99869	WGS84	BC29821	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29821_Rhar_Sim2018_COI	BOTA5	1	BC29821_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29821	Sample	BC29821	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA5	COI	45	BC29821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA5	CTGCTGCTATTGCTCATG	151.20803
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29823_Aamu_Bax2006_COI	BOTA3	1	BC29823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	GCACAACCGGGATCTTTACTTC	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29823_Pcan_Bot2011_IGS	BOTA3	1	BC29823_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA3	IGS	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	CGTAATCCTCAGTACTGGCTA	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29823_Pper_Dia2013_COI	BOTA3	1	BC29823_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	CTTAGTGGCATTAATTCGDAATCC	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29823_Sspa_Oph2007_COI	BOTA3	1	BC29823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	GAAGGCCTTCCGATGATC	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29823_Upin_Bot2011_COI	BOTA3	1	BC29823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	CTTTAATTACAGCGTTTTTATTGTTGT	151.20712
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29824_Cjap_Sim2018_COI	BOTA1	1	BC29824_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29824_Esin_And2018_Cyb	BOTA1	1	BC29824_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA1	Cytb	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	ACCCCTCCTCATATCCAACCA	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.67	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29824_Mgig_Bot2012_COI	BOTA1	1	BC29824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	TCTTATTCGTTGGAGACTTTATAACCCT	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29824_Mjap_Gib2022_COI	BOTA1	1	BC29824_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	AATTTAATTGTGACTAGGCATGGTCTG	151.20991
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29825_Cmae_Bot2011_COI	BOTA2	1	BC29825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	ATGAACAGTCTATCCTCCTTTAG	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29825_Hsan_Wil2021_COI	BOTA2	1	BC29825_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	CCTGGGCCGGTATAGTAGGT	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29825_Mros_Gib2022_COI	BOTA2	1	BC29825_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29825_Msal_Bot2012_COI	BOTA2	1	BC29825_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	GYTAGTTCCRATGATGTTAGCTG	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29825_Pvir_Dia2013_COI	BOTA2	1	BC29825_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	CTTAGTGGCATTAATTCGDAATCC	151.21153
BC29825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00124	WGS84	BC29825	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29825_Rhar_Sim2018_COI	BOTA2	1	BC29825_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29825	Sample	BC29825	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA2	COI	45	BC29825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA2	CTGCTGCTATTGCTCATG	151.21153
BC29827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67287	WGS84	BC29827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29827_Aamu_Bax2006_COI	WEIA19	1	BC29827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29827	Sample	BC29827	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA19	COI	45	BC29827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA19	GCACAACCGGGATCTTTACTTC	141.86564
BC29827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67287	WGS84	BC29827	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29827_Pcan_Bot2011_IGS	WEIA19	1	BC29827_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29827	Sample	BC29827	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA19	IGS	45	BC29827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA19	CGTAATCCTCAGTACTGGCTA	141.86564
BC29827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67287	WGS84	BC29827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29827_Sspa_Oph2007_COI	WEIA19	1	BC29827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29827	Sample	BC29827	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA19	COI	45	BC29827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA19	GAAGGCCTTCCGATGATC	141.86564
BC29827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67287	WGS84	BC29827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29827_Upin_Bot2011_COI	WEIA19	1	BC29827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29827	Sample	BC29827	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA19	COI	45	BC29827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA19	CTTTAATTACAGCGTTTTTATTGTTGT	141.86564
BC29828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67224	WGS84	BC29828	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29828_Mgig_Bot2012_COI	WEIA18	1	BC29828_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29828	Sample	BC29828	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA18	COI	45	BC29828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA18	TCTTATTCGTTGGAGACTTTATAACCCT	141.86659
BC29829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67328	WGS84	BC29829	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29829_Cmae_Bot2011_COI	WEIA17	1	BC29829_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29829	Sample	BC29829	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA17	COI	45	BC29829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA17	ATGAACAGTCTATCCTCCTTTAG	141.87218
BC29829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67328	WGS84	BC29829	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29829_Msal_Bot2012_COI	WEIA17	1	BC29829_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29829	Sample	BC29829	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA17	COI	45	BC29829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA17	GYTAGTTCCRATGATGTTAGCTG	141.87218
BC29830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67318	WGS84	BC29830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29830_Aamu_Bax2006_COI	WEIA16	1	BC29830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29830	Sample	BC29830	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA16	COI	45	BC29830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA16	GCACAACCGGGATCTTTACTTC	141.86865
BC29830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67318	WGS84	BC29830	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29830_Pcan_Bot2011_IGS	WEIA16	1	BC29830_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29830	Sample	BC29830	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA16	IGS	45	BC29830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA16	CGTAATCCTCAGTACTGGCTA	141.86865
BC29830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67318	WGS84	BC29830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29830_Sspa_Oph2007_COI	WEIA16	1	BC29830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29830	Sample	BC29830	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA16	COI	45	BC29830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA16	GAAGGCCTTCCGATGATC	141.86865
BC29830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67318	WGS84	BC29830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29830_Upin_Bot2011_COI	WEIA16	1	BC29830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29830	Sample	BC29830	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA16	COI	45	BC29830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA16	CTTTAATTACAGCGTTTTTATTGTTGT	141.86865
BC29831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67395	WGS84	BC29831	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29831_Mgig_Bot2012_COI	WEIA14	1	BC29831_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29831	Sample	BC29831	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA14	COI	45	BC29831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA14	TCTTATTCGTTGGAGACTTTATAACCCT	141.8665
BC29832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67448	WGS84	BC29832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29832_Cmae_Bot2011_COI	WEIA13	1	BC29832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29832	Sample	BC29832	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA13	COI	45	BC29832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA13	ATGAACAGTCTATCCTCCTTTAG	141.87165
BC29832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67448	WGS84	BC29832	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29832_Msal_Bot2012_COI	WEIA13	1	BC29832_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29832	Sample	BC29832	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA13	COI	45	BC29832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA13	GYTAGTTCCRATGATGTTAGCTG	141.87165
BC29834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67501	WGS84	BC29834	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29834_Aamu_Bax2006_COI	WEIA11	1	BC29834_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29834	Sample	BC29834	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA11	COI	45	BC29834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA11	GCACAACCGGGATCTTTACTTC	141.86849
BC29834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67501	WGS84	BC29834	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29834_Pcan_Bot2011_IGS	WEIA11	1	BC29834_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29834	Sample	BC29834	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA11	IGS	45	BC29834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA11	CGTAATCCTCAGTACTGGCTA	141.86849
BC29834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67501	WGS84	BC29834	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29834_Sspa_Oph2007_COI	WEIA11	1	BC29834_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29834	Sample	BC29834	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA11	COI	45	BC29834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA11	GAAGGCCTTCCGATGATC	141.86849
BC29834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67501	WGS84	BC29834	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29834_Upin_Bot2011_COI	WEIA11	1	BC29834_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29834	Sample	BC29834	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA11	COI	45	BC29834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA11	CTTTAATTACAGCGTTTTTATTGTTGT	141.86849
BC29835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66819	WGS84	BC29835	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29835_Mgig_Bot2012_COI	WEIA9	1	BC29835_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29835	Sample	BC29835	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA9	COI	45	BC29835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA9	TCTTATTCGTTGGAGACTTTATAACCCT	141.86086
BC29836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66946	WGS84	BC29836	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29836_Cmae_Bot2011_COI	WEIA8	1	BC29836_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29836	Sample	BC29836	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA8	COI	45	BC29836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA8	ATGAACAGTCTATCCTCCTTTAG	141.86323
BC29836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66946	WGS84	BC29836	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29836_Msal_Bot2012_COI	WEIA8	1	BC29836_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29836	Sample	BC29836	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA8	COI	45	BC29836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA8	GYTAGTTCCRATGATGTTAGCTG	141.86323
BC29838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66604	WGS84	BC29838	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29838_Aamu_Bax2006_COI	WEIA4	1	BC29838_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29838	Sample	BC29838	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA4	COI	45	BC29838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA4	GCACAACCGGGATCTTTACTTC	141.84772
BC29838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66604	WGS84	BC29838	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29838_Pcan_Bot2011_IGS	WEIA4	1	BC29838_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29838	Sample	BC29838	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA4	IGS	45	BC29838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA4	CGTAATCCTCAGTACTGGCTA	141.84772
BC29838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66604	WGS84	BC29838	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29838_Sspa_Oph2007_COI	WEIA4	1	BC29838_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29838	Sample	BC29838	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA4	COI	45	BC29838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA4	GAAGGCCTTCCGATGATC	141.84772
BC29838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66604	WGS84	BC29838	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29838_Upin_Bot2011_COI	WEIA4	1	BC29838_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29838	Sample	BC29838	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA4	COI	45	BC29838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA4	CTTTAATTACAGCGTTTTTATTGTTGT	141.84772
BC29839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66715	WGS84	BC29839	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29839_Mgig_Bot2012_COI	WEIA3	1	BC29839_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29839	Sample	BC29839	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA3	COI	45	BC29839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA3	TCTTATTCGTTGGAGACTTTATAACCCT	141.84948
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-12.20676	WGS84	BC29846	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29846_Asen_Wil2025_COI	GOVA15	1	BC29846_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Asen_Wil2025_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	GTTGTTGTTACAACACATGCTCTTTTG	136.66753
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20676	WGS84	BC29846	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29846_Mgig_Bot2012_COI	GOVA15	1	BC29846_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	TCTTATTCGTTGGAGACTTTATAACCCT	136.66753
BC29847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20909	WGS84	BC29847	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29847_Cmae_Bot2011_COI	GOVA10	1	BC29847_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29847	Sample	BC29847	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA10	COI	45	BC29847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA10	ATGAACAGTCTATCCTCCTTTAG	136.66891
BC29847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20909	WGS84	BC29847	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29847_Msal_Bot2012_COI	GOVA10	1	BC29847_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29847	Sample	BC29847	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA10	COI	45	BC29847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA10	GYTAGTTCCRATGATGTTAGCTG	136.66891
BC29849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20293	WGS84	BC29849	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29849_Aamu_Bax2006_COI	GOVA22	1	BC29849_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29849	Sample	BC29849	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA22	COI	45	BC29849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA22	GCACAACCGGGATCTTTACTTC	136.66973
BC29849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20293	WGS84	BC29849	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29849_Pcan_Bot2011_IGS	GOVA22	1	BC29849_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29849	Sample	BC29849	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA22	IGS	45	BC29849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA22	CGTAATCCTCAGTACTGGCTA	136.66973
BC29849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20293	WGS84	BC29849	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29849_Sspa_Oph2007_COI	GOVA22	1	BC29849_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29849	Sample	BC29849	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA22	COI	45	BC29849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA22	GAAGGCCTTCCGATGATC	136.66973
BC29849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20293	WGS84	BC29849	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29849_Upin_Bot2011_COI	GOVA22	1	BC29849_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29849	Sample	BC29849	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA22	COI	45	BC29849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA22	CTTTAATTACAGCGTTTTTATTGTTGT	136.66973
BC29850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20397	WGS84	BC29850	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29850_Cmae_Bot2011_COI	GOVA21	1	BC29850_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29850	Sample	BC29850	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA21	COI	45	BC29850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA21	ATGAACAGTCTATCCTCCTTTAG	136.67036
BC29850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20397	WGS84	BC29850	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29850_Msal_Bot2012_COI	GOVA21	1	BC29850_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29850	Sample	BC29850	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA21	COI	45	BC29850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA21	GYTAGTTCCRATGATGTTAGCTG	136.67036
BC29852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20625	WGS84	BC29852	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29852_Aamu_Bax2006_COI	GOVA17	1	BC29852_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29852	Sample	BC29852	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA17	COI	45	BC29852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA17	GCACAACCGGGATCTTTACTTC	136.6721
BC29852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20625	WGS84	BC29852	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29852_Pcan_Bot2011_IGS	GOVA17	1	BC29852_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29852	Sample	BC29852	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA17	IGS	45	BC29852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA17	CGTAATCCTCAGTACTGGCTA	136.6721
BC29852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20625	WGS84	BC29852	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29852_Sspa_Oph2007_COI	GOVA17	1	BC29852_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29852	Sample	BC29852	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA17	COI	45	BC29852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA17	GAAGGCCTTCCGATGATC	136.6721
BC29852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20625	WGS84	BC29852	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29852_Upin_Bot2011_COI	GOVA17	1	BC29852_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29852	Sample	BC29852	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA17	COI	45	BC29852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA17	CTTTAATTACAGCGTTTTTATTGTTGT	136.6721
BC29853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20777	WGS84	BC29853	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29853_Mgig_Bot2012_COI	GOVA13	1	BC29853_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29853	Sample	BC29853	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA13	COI	45	BC29853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA13	TCTTATTCGTTGGAGACTTTATAACCCT	136.66944
BC29854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20891	WGS84	BC29854	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29854_Cmae_Bot2011_COI	GOVA11	1	BC29854_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29854	Sample	BC29854	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA11	COI	45	BC29854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA11	ATGAACAGTCTATCCTCCTTTAG	136.67007
BC29854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20891	WGS84	BC29854	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29854_Msal_Bot2012_COI	GOVA11	1	BC29854_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29854	Sample	BC29854	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA11	COI	45	BC29854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA11	GYTAGTTCCRATGATGTTAGCTG	136.67007
BC29856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20147	WGS84	BC29856	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29856_Aamu_Bax2006_COI	GOVA7	1	BC29856_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29856	Sample	BC29856	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA7	COI	45	BC29856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA7	GCACAACCGGGATCTTTACTTC	136.68073
BC29856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20147	WGS84	BC29856	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29856_Pcan_Bot2011_IGS	GOVA7	1	BC29856_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29856	Sample	BC29856	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA7	IGS	45	BC29856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA7	CGTAATCCTCAGTACTGGCTA	136.68073
BC29856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20147	WGS84	BC29856	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29856_Sspa_Oph2007_COI	GOVA7	1	BC29856_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29856	Sample	BC29856	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA7	COI	45	BC29856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA7	GAAGGCCTTCCGATGATC	136.68073
BC29856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20147	WGS84	BC29856	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29856_Upin_Bot2011_COI	GOVA7	1	BC29856_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29856	Sample	BC29856	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA7	COI	45	BC29856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA7	CTTTAATTACAGCGTTTTTATTGTTGT	136.68073
BC29857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.2028	WGS84	BC29857	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29857_Mgig_Bot2012_COI	GOVA6	1	BC29857_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29857	Sample	BC29857	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA6	COI	45	BC29857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA6	TCTTATTCGTTGGAGACTTTATAACCCT	136.68155
BC29858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20648	WGS84	BC29858	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29858_Cmae_Bot2011_COI	GOVA3	1	BC29858_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29858	Sample	BC29858	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA3	COI	45	BC29858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA3	ATGAACAGTCTATCCTCCTTTAG	136.68
BC29858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20648	WGS84	BC29858	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29858_Msal_Bot2012_COI	GOVA3	1	BC29858_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29858	Sample	BC29858	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA3	COI	45	BC29858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA3	GYTAGTTCCRATGATGTTAGCTG	136.68
BC29860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19556	WGS84	BC29860	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29860_Mgig_Bot2012_COI	GOVA1	1	BC29860_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29860	Sample	BC29860	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA1	COI	45	BC29860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA1	TCTTATTCGTTGGAGACTTTATAACCCT	136.68512
BC37387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99876	WGS84	BC37387	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37387_Aamu_Bax2006_COI	BOTB6	1	BC37387_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37387	Sample	BC37387	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB6	COI	45	BC37387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB6	GCACAACCGGGATCTTTACTTC	151.2101
BC37387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99876	WGS84	BC37387	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37387_Pcan_Bot2011_IGS	BOTB6	1	BC37387_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37387	Sample	BC37387	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB6	IGS	45	BC37387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB6	CGTAATCCTCAGTACTGGCTA	151.2101
BC37387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99876	WGS84	BC37387	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37387_Sspa_Oph2007_COI	BOTB6	1	BC37387_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37387	Sample	BC37387	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB6	COI	45	BC37387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB6	GAAGGCCTTCCGATGATC	151.2101
BC37387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99876	WGS84	BC37387	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37387_Upin_Bot2011_COI	BOTB6	1	BC37387_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37387	Sample	BC37387	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB6	COI	45	BC37387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB6	CTTTAATTACAGCGTTTTTATTGTTGT	151.2101
BC37388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99816	WGS84	BC37388	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37388_Mgig_Bot2012_COI	BOTB7	1	BC37388_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37388	Sample	BC37388	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB7	COI	45	BC37388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB7	TCTTATTCGTTGGAGACTTTATAACCCT	151.21204
BC37389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99727	WGS84	BC37389	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37389_Cmae_Bot2011_COI	BOTB8	1	BC37389_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37389	Sample	BC37389	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB8	COI	45	BC37389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB8	ATGAACAGTCTATCCTCCTTTAG	151.20967
BC37389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99727	WGS84	BC37389	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37389_Msal_Bot2012_COI	BOTB8	1	BC37389_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37389	Sample	BC37389	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB8	COI	45	BC37389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB8	GYTAGTTCCRATGATGTTAGCTG	151.20967
BC37390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99718	WGS84	BC37390	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37390_Aamu_Bax2006_COI	BOTB9	1	BC37390_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37390	Sample	BC37390	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB9	COI	45	BC37390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB9	GCACAACCGGGATCTTTACTTC	151.21115
BC37390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99718	WGS84	BC37390	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37390_Pcan_Bot2011_IGS	BOTB9	1	BC37390_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37390	Sample	BC37390	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB9	IGS	45	BC37390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB9	CGTAATCCTCAGTACTGGCTA	151.21115
BC37390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99718	WGS84	BC37390	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37390_Sspa_Oph2007_COI	BOTB9	1	BC37390_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37390	Sample	BC37390	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB9	COI	45	BC37390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB9	GAAGGCCTTCCGATGATC	151.21115
BC37390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99718	WGS84	BC37390	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37390_Upin_Bot2011_COI	BOTB9	1	BC37390_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37390	Sample	BC37390	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB9	COI	45	BC37390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB9	CTTTAATTACAGCGTTTTTATTGTTGT	151.21115
BC37391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98273	WGS84	BC37391	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37391_Mgig_Bot2012_COI	BOTB10	1	BC37391_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37391	Sample	BC37391	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB10	COI	45	BC37391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB10	TCTTATTCGTTGGAGACTTTATAACCCT	151.20824
BC37392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98116	WGS84	BC37392	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37392_Cmae_Bot2011_COI	BOTB11	1	BC37392_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37392	Sample	BC37392	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB11	COI	45	BC37392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB11	ATGAACAGTCTATCCTCCTTTAG	151.20848
BC37392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98116	WGS84	BC37392	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37392_Msal_Bot2012_COI	BOTB11	1	BC37392_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37392	Sample	BC37392	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB11	COI	45	BC37392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB11	GYTAGTTCCRATGATGTTAGCTG	151.20848
BC37394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97853	WGS84	BC37394	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37394_Aamu_Bax2006_COI	BOTB13	1	BC37394_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37394	Sample	BC37394	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB13	COI	45	BC37394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB13	GCACAACCGGGATCTTTACTTC	151.20963
BC37394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97853	WGS84	BC37394	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37394_Pcan_Bot2011_IGS	BOTB13	1	BC37394_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37394	Sample	BC37394	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB13	IGS	45	BC37394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB13	CGTAATCCTCAGTACTGGCTA	151.20963
BC37394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97853	WGS84	BC37394	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37394_Sspa_Oph2007_COI	BOTB13	1	BC37394_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37394	Sample	BC37394	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB13	COI	45	BC37394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB13	GAAGGCCTTCCGATGATC	151.20963
BC37394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97853	WGS84	BC37394	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37394_Upin_Bot2011_COI	BOTB13	1	BC37394_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37394	Sample	BC37394	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB13	COI	45	BC37394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB13	CTTTAATTACAGCGTTTTTATTGTTGT	151.20963
BC37395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9773	WGS84	BC37395	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37395_Mgig_Bot2012_COI	BOTB14	1	BC37395_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37395	Sample	BC37395	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB14	COI	45	BC37395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB14	TCTTATTCGTTGGAGACTTTATAACCCT	151.20861
BC37396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97617	WGS84	BC37396	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37396_Cmae_Bot2011_COI	BOTB15	1	BC37396_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37396	Sample	BC37396	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB15	COI	45	BC37396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB15	ATGAACAGTCTATCCTCCTTTAG	151.20929
BC37396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97617	WGS84	BC37396	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37396_Msal_Bot2012_COI	BOTB15	1	BC37396_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37396	Sample	BC37396	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB15	COI	45	BC37396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB15	GYTAGTTCCRATGATGTTAGCTG	151.20929
BC37398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97457	WGS84	BC37398	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37398_Aamu_Bax2006_COI	BOTB17	1	BC37398_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37398	Sample	BC37398	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB17	COI	45	BC37398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB17	GCACAACCGGGATCTTTACTTC	151.2098
BC37398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97457	WGS84	BC37398	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37398_Pcan_Bot2011_IGS	BOTB17	1	BC37398_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37398	Sample	BC37398	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB17	IGS	45	BC37398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB17	CGTAATCCTCAGTACTGGCTA	151.2098
BC37398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97457	WGS84	BC37398	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37398_Sspa_Oph2007_COI	BOTB17	1	BC37398_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37398	Sample	BC37398	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB17	COI	45	BC37398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB17	GAAGGCCTTCCGATGATC	151.2098
BC37398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97457	WGS84	BC37398	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37398_Upin_Bot2011_COI	BOTB17	1	BC37398_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37398	Sample	BC37398	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB17	COI	45	BC37398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB17	CTTTAATTACAGCGTTTTTATTGTTGT	151.2098
BC37399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97298	WGS84	BC37399	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37399_Mgig_Bot2012_COI	BOTB18	1	BC37399_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37399	Sample	BC37399	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB18	COI	45	BC37399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB18	TCTTATTCGTTGGAGACTTTATAACCCT	151.20234
BC37400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97257	WGS84	BC37400	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37400_Cmae_Bot2011_COI	BOTB19	1	BC37400_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37400	Sample	BC37400	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB19	COI	45	BC37400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB19	ATGAACAGTCTATCCTCCTTTAG	151.20432
BC37400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97257	WGS84	BC37400	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37400_Msal_Bot2012_COI	BOTB19	1	BC37400_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37400	Sample	BC37400	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB19	COI	45	BC37400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB19	GYTAGTTCCRATGATGTTAGCTG	151.20432
BC37402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97291	WGS84	BC37402	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37402_Aamu_Bax2006_COI	BOTB21	1	BC37402_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37402	Sample	BC37402	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB21	COI	45	BC37402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB21	GCACAACCGGGATCTTTACTTC	151.20878
BC37402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97291	WGS84	BC37402	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37402_Pcan_Bot2011_IGS	BOTB21	1	BC37402_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37402	Sample	BC37402	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB21	IGS	45	BC37402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB21	CGTAATCCTCAGTACTGGCTA	151.20878
BC37402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97291	WGS84	BC37402	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37402_Sspa_Oph2007_COI	BOTB21	1	BC37402_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37402	Sample	BC37402	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB21	COI	45	BC37402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB21	GAAGGCCTTCCGATGATC	151.20878
BC37402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97291	WGS84	BC37402	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37402_Upin_Bot2011_COI	BOTB21	1	BC37402_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37402	Sample	BC37402	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB21	COI	45	BC37402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB21	CTTTAATTACAGCGTTTTTATTGTTGT	151.20878
BC37403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97278	WGS84	BC37403	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37403_Mgig_Bot2012_COI	BOTB22	1	BC37403_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37403	Sample	BC37403	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB22	COI	45	BC37403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB22	TCTTATTCGTTGGAGACTTTATAACCCT	151.21048
BC37404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97176	WGS84	BC37404	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37404_Cmae_Bot2011_COI	BOTB23	1	BC37404_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37404	Sample	BC37404	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB23	COI	45	BC37404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB23	ATGAACAGTCTATCCTCCTTTAG	151.19977
BC37404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97176	WGS84	BC37404	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37404_Msal_Bot2012_COI	BOTB23	1	BC37404_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37404	Sample	BC37404	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB23	COI	45	BC37404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB23	GYTAGTTCCRATGATGTTAGCTG	151.19977
BC37406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9714	WGS84	BC37406	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37406_Aamu_Bax2006_COI	BOTB25	1	BC37406_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37406	Sample	BC37406	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB25	COI	45	BC37406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB25	GCACAACCGGGATCTTTACTTC	151.20539
BC37406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9714	WGS84	BC37406	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37406_Pcan_Bot2011_IGS	BOTB25	1	BC37406_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37406	Sample	BC37406	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB25	IGS	45	BC37406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB25	CGTAATCCTCAGTACTGGCTA	151.20539
BC37406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9714	WGS84	BC37406	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.303	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37406_Sspa_Oph2007_COI	BOTB25	1	BC37406_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37406	Sample	BC37406	targeted	Present	Sample	Sspa_Oph2007_COI	BOTB25	COI	45	BC37406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB25	GAAGGCCTTCCGATGATC	151.20539
BC37406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9714	WGS84	BC37406	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37406_Upin_Bot2011_COI	BOTB25	1	BC37406_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37406	Sample	BC37406	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB25	COI	45	BC37406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB25	CTTTAATTACAGCGTTTTTATTGTTGT	151.20539
BC37407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97093	WGS84	BC37407	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37407_Mgig_Bot2012_COI	BOTB26	1	BC37407_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37407	Sample	BC37407	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB26	COI	45	BC37407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB26	TCTTATTCGTTGGAGACTTTATAACCCT	151.20754
BC37408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97067	WGS84	BC37408	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37408_Cmae_Bot2011_COI	BOTB27	1	BC37408_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37408	Sample	BC37408	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB27	COI	45	BC37408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB27	ATGAACAGTCTATCCTCCTTTAG	151.20974
BC37408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97067	WGS84	BC37408	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37408_Msal_Bot2012_COI	BOTB27	1	BC37408_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37408	Sample	BC37408	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB27	COI	45	BC37408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB27	GYTAGTTCCRATGATGTTAGCTG	151.20974
BC37410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97018	WGS84	BC37410	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37410_Mgig_Bot2012_COI	BOTB29	1	BC37410_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37410	Sample	BC37410	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB29	COI	45	BC37410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB29	TCTTATTCGTTGGAGACTTTATAACCCT	151.21416
BC37411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96986	WGS84	BC37411	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37411_Cmae_Bot2011_COI	BOTB30	1	BC37411_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37411	Sample	BC37411	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB30	COI	45	BC37411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB30	ATGAACAGTCTATCCTCCTTTAG	151.21632
BC37411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96986	WGS84	BC37411	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37411_Msal_Bot2012_COI	BOTB30	1	BC37411_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37411	Sample	BC37411	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB30	COI	45	BC37411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB30	GYTAGTTCCRATGATGTTAGCTG	151.21632
BC37413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97061	WGS84	BC37413	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37413_Aamu_Bax2006_COI	BOTB32	1	BC37413_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37413	Sample	BC37413	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB32	COI	45	BC37413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB32	GCACAACCGGGATCTTTACTTC	151.20139
BC37413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97061	WGS84	BC37413	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37413_Pcan_Bot2011_IGS	BOTB32	1	BC37413_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37413	Sample	BC37413	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB32	IGS	45	BC37413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB32	CGTAATCCTCAGTACTGGCTA	151.20139
BC37413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97061	WGS84	BC37413	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37413_Sspa_Oph2007_COI	BOTB32	1	BC37413_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37413	Sample	BC37413	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB32	COI	45	BC37413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB32	GAAGGCCTTCCGATGATC	151.20139
BC37413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97061	WGS84	BC37413	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37413_Upin_Bot2011_COI	BOTB32	1	BC37413_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37413	Sample	BC37413	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB32	COI	45	BC37413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB32	CTTTAATTACAGCGTTTTTATTGTTGT	151.20139
BC37414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9686	WGS84	BC37414	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37414_Mgig_Bot2012_COI	BOTB33	1	BC37414_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37414	Sample	BC37414	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB33	COI	45	BC37414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB33	TCTTATTCGTTGGAGACTTTATAACCCT	151.21507
BC37415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9682	WGS84	BC37415	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37415_Cmae_Bot2011_COI	BOTB34	1	BC37415_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37415	Sample	BC37415	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB34	COI	45	BC37415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB34	ATGAACAGTCTATCCTCCTTTAG	151.21721
BC37415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9682	WGS84	BC37415	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37415_Msal_Bot2012_COI	BOTB34	1	BC37415_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37415	Sample	BC37415	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB34	COI	45	BC37415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB34	GYTAGTTCCRATGATGTTAGCTG	151.21721
BC37417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9683	WGS84	BC37417	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37417_Aamu_Bax2006_COI	BOTB36	1	BC37417_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37417	Sample	BC37417	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB36	COI	45	BC37417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB36	GCACAACCGGGATCTTTACTTC	151.19945
BC37417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9683	WGS84	BC37417	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37417_Pcan_Bot2011_IGS	BOTB36	1	BC37417_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37417	Sample	BC37417	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB36	IGS	45	BC37417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB36	CGTAATCCTCAGTACTGGCTA	151.19945
BC37417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9683	WGS84	BC37417	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37417_Sspa_Oph2007_COI	BOTB36	1	BC37417_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37417	Sample	BC37417	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB36	COI	45	BC37417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB36	GAAGGCCTTCCGATGATC	151.19945
BC37417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9683	WGS84	BC37417	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37417_Upin_Bot2011_COI	BOTB36	1	BC37417_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37417	Sample	BC37417	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB36	COI	45	BC37417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB36	CTTTAATTACAGCGTTTTTATTGTTGT	151.19945
BC37418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96688	WGS84	BC37418	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37418_Mgig_Bot2012_COI	BOTB37	1	BC37418_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37418	Sample	BC37418	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB37	COI	45	BC37418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB37	TCTTATTCGTTGGAGACTTTATAACCCT	151.19813
BC37419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96582	WGS84	BC37419	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37419_Cmae_Bot2011_COI	BOTB38	1	BC37419_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37419	Sample	BC37419	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB38	COI	45	BC37419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB38	ATGAACAGTCTATCCTCCTTTAG	151.19983
BC37419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96582	WGS84	BC37419	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37419_Msal_Bot2012_COI	BOTB38	1	BC37419_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37419	Sample	BC37419	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB38	COI	45	BC37419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB38	GYTAGTTCCRATGATGTTAGCTG	151.19983
BC37420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96368	WGS84	BC37420	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37420_Aamu_Bax2006_COI	BOTB39	1	BC37420_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37420	Sample	BC37420	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB39	COI	45	BC37420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB39	GCACAACCGGGATCTTTACTTC	151.19844
BC37420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96368	WGS84	BC37420	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37420_Pcan_Bot2011_IGS	BOTB39	1	BC37420_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37420	Sample	BC37420	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB39	IGS	45	BC37420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB39	CGTAATCCTCAGTACTGGCTA	151.19844
BC37420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96368	WGS84	BC37420	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37420_Sspa_Oph2007_COI	BOTB39	1	BC37420_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37420	Sample	BC37420	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB39	COI	45	BC37420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB39	GAAGGCCTTCCGATGATC	151.19844
BC37420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96368	WGS84	BC37420	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37420_Upin_Bot2011_COI	BOTB39	1	BC37420_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37420	Sample	BC37420	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB39	COI	45	BC37420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB39	CTTTAATTACAGCGTTTTTATTGTTGT	151.19844
BC37421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.95928	WGS84	BC37421	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37421_Mgig_Bot2012_COI	BOTB42	1	BC37421_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37421	Sample	BC37421	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB42	COI	45	BC37421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB42	TCTTATTCGTTGGAGACTTTATAACCCT	151.19883
BC37422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96071	WGS84	BC37422	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37422_Cmae_Bot2011_COI	BOTB41	1	BC37422_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37422	Sample	BC37422	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB41	COI	45	BC37422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB41	ATGAACAGTCTATCCTCCTTTAG	151.19803
BC37422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96071	WGS84	BC37422	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37422_Msal_Bot2012_COI	BOTB41	1	BC37422_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37422	Sample	BC37422	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB41	COI	45	BC37422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB41	GYTAGTTCCRATGATGTTAGCTG	151.19803
BC37424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96267	WGS84	BC37424	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37424_Aamu_Bax2006_COI	BOTB40	1	BC37424_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37424	Sample	BC37424	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB40	COI	45	BC37424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB40	GCACAACCGGGATCTTTACTTC	151.19922
BC37424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96267	WGS84	BC37424	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37424_Pcan_Bot2011_IGS	BOTB40	1	BC37424_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37424	Sample	BC37424	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB40	IGS	45	BC37424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB40	CGTAATCCTCAGTACTGGCTA	151.19922
BC37424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96267	WGS84	BC37424	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37424_Sspa_Oph2007_COI	BOTB40	1	BC37424_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37424	Sample	BC37424	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB40	COI	45	BC37424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB40	GAAGGCCTTCCGATGATC	151.19922
BC37424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96267	WGS84	BC37424	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37424_Upin_Bot2011_COI	BOTB40	1	BC37424_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37424	Sample	BC37424	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB40	COI	45	BC37424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB40	CTTTAATTACAGCGTTTTTATTGTTGT	151.19922
BC37426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8814	WGS84	BC37426	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37426_Cmae_Bot2011_COI	NEWB46	1	BC37426_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37426	Sample	BC37426	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB46	COI	45	BC37426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB46	ATGAACAGTCTATCCTCCTTTAG	151.75162
BC37426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8814	WGS84	BC37426	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37426_Msal_Bot2012_COI	NEWB46	1	BC37426_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37426	Sample	BC37426	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB46	COI	45	BC37426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB46	GYTAGTTCCRATGATGTTAGCTG	151.75162
BC37428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88286	WGS84	BC37428	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37428_Aamu_Bax2006_COI	NEWB44	1	BC37428_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37428	Sample	BC37428	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB44	COI	45	BC37428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB44	GCACAACCGGGATCTTTACTTC	151.75313
BC37428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88286	WGS84	BC37428	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37428_Pcan_Bot2011_IGS	NEWB44	1	BC37428_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37428	Sample	BC37428	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB44	IGS	45	BC37428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB44	CGTAATCCTCAGTACTGGCTA	151.75313
BC37428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88286	WGS84	BC37428	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37428_Sspa_Oph2007_COI	NEWB44	1	BC37428_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37428	Sample	BC37428	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB44	COI	45	BC37428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB44	GAAGGCCTTCCGATGATC	151.75313
BC37428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88286	WGS84	BC37428	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37428_Upin_Bot2011_COI	NEWB44	1	BC37428_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37428	Sample	BC37428	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB44	COI	45	BC37428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB44	CTTTAATTACAGCGTTTTTATTGTTGT	151.75313
BC37429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88427	WGS84	BC37429	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.991	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37429_Mgig_Bot2012_COI	NEWB43	1	BC37429_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37429	Sample	BC37429	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB43	COI	45	BC37429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB43	TCTTATTCGTTGGAGACTTTATAACCCT	151.75678
BC37431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88566	WGS84	BC37431	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37431_Aamu_Bax2006_COI	NEWB41	1	BC37431_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37431	Sample	BC37431	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB41	COI	45	BC37431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB41	GCACAACCGGGATCTTTACTTC	151.75829
BC37431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88566	WGS84	BC37431	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37431_Pcan_Bot2011_IGS	NEWB41	1	BC37431_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37431	Sample	BC37431	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB41	IGS	45	BC37431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB41	CGTAATCCTCAGTACTGGCTA	151.75829
BC37431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88566	WGS84	BC37431	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37431_Sspa_Oph2007_COI	NEWB41	1	BC37431_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37431	Sample	BC37431	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB41	COI	45	BC37431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB41	GAAGGCCTTCCGATGATC	151.75829
BC37431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88566	WGS84	BC37431	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37431_Upin_Bot2011_COI	NEWB41	1	BC37431_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37431	Sample	BC37431	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB41	COI	45	BC37431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB41	CTTTAATTACAGCGTTTTTATTGTTGT	151.75829
BC37432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88519	WGS84	BC37432	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.142	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37432_Mgig_Bot2012_COI	NEWB40	1	BC37432_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37432	Sample	BC37432	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB40	COI	45	BC37432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB40	TCTTATTCGTTGGAGACTTTATAACCCT	151.75593
BC37433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88695	WGS84	BC37433	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37433_Cmae_Bot2011_COI	NEWB39	1	BC37433_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37433	Sample	BC37433	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB39	COI	45	BC37433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB39	ATGAACAGTCTATCCTCCTTTAG	151.76199
BC37433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88695	WGS84	BC37433	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37433_Msal_Bot2012_COI	NEWB39	1	BC37433_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37433	Sample	BC37433	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB39	COI	45	BC37433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB39	GYTAGTTCCRATGATGTTAGCTG	151.76199
BC37435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88803	WGS84	BC37435	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37435_Aamu_Bax2006_COI	NEWB37	1	BC37435_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37435	Sample	BC37435	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB37	COI	45	BC37435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB37	GCACAACCGGGATCTTTACTTC	151.76579
BC37435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88803	WGS84	BC37435	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37435_Pcan_Bot2011_IGS	NEWB37	1	BC37435_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37435	Sample	BC37435	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB37	IGS	45	BC37435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB37	CGTAATCCTCAGTACTGGCTA	151.76579
BC37435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88803	WGS84	BC37435	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37435_Sspa_Oph2007_COI	NEWB37	1	BC37435_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37435	Sample	BC37435	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB37	COI	45	BC37435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB37	GAAGGCCTTCCGATGATC	151.76579
BC37435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88803	WGS84	BC37435	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37435_Upin_Bot2011_COI	NEWB37	1	BC37435_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37435	Sample	BC37435	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB37	COI	45	BC37435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB37	CTTTAATTACAGCGTTTTTATTGTTGT	151.76579
BC37436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88799	WGS84	BC37436	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.18	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37436_Mgig_Bot2012_COI	NEWB36	1	BC37436_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37436	Sample	BC37436	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB36	COI	45	BC37436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB36	TCTTATTCGTTGGAGACTTTATAACCCT	151.76335
BC37437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8881	WGS84	BC37437	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37437_Cmae_Bot2011_COI	NEWB35	1	BC37437_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37437	Sample	BC37437	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB35	COI	45	BC37437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB35	ATGAACAGTCTATCCTCCTTTAG	151.7608
BC37437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8881	WGS84	BC37437	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37437_Msal_Bot2012_COI	NEWB35	1	BC37437_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37437	Sample	BC37437	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB35	COI	45	BC37437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB35	GYTAGTTCCRATGATGTTAGCTG	151.7608
BC37439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88954	WGS84	BC37439	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37439_Aamu_Bax2006_COI	NEWB33	1	BC37439_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37439	Sample	BC37439	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB33	COI	45	BC37439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB33	GCACAACCGGGATCTTTACTTC	151.76725
BC37439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88954	WGS84	BC37439	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37439_Pcan_Bot2011_IGS	NEWB33	1	BC37439_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37439	Sample	BC37439	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB33	IGS	45	BC37439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB33	CGTAATCCTCAGTACTGGCTA	151.76725
BC37439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88954	WGS84	BC37439	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37439_Sspa_Oph2007_COI	NEWB33	1	BC37439_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37439	Sample	BC37439	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB33	COI	45	BC37439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB33	GAAGGCCTTCCGATGATC	151.76725
BC37439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88954	WGS84	BC37439	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37439_Upin_Bot2011_COI	NEWB33	1	BC37439_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37439	Sample	BC37439	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB33	COI	45	BC37439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB33	CTTTAATTACAGCGTTTTTATTGTTGT	151.76725
BC37440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88948	WGS84	BC37440	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37440_Cmae_Bot2011_COI	NEWB32	1	BC37440_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37440	Sample	BC37440	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB32	COI	45	BC37440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB32	ATGAACAGTCTATCCTCCTTTAG	151.76464
BC37440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88948	WGS84	BC37440	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37440_Msal_Bot2012_COI	NEWB32	1	BC37440_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37440	Sample	BC37440	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB32	COI	45	BC37440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB32	GYTAGTTCCRATGATGTTAGCTG	151.76464
BC37442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89074	WGS84	BC37442	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37442_Aamu_Bax2006_COI	NEWB30	1	BC37442_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37442	Sample	BC37442	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB30	COI	45	BC37442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB30	GCACAACCGGGATCTTTACTTC	151.77097
BC37442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89074	WGS84	BC37442	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37442_Pcan_Bot2011_IGS	NEWB30	1	BC37442_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37442	Sample	BC37442	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB30	IGS	45	BC37442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB30	CGTAATCCTCAGTACTGGCTA	151.77097
BC37442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89074	WGS84	BC37442	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37442_Sspa_Oph2007_COI	NEWB30	1	BC37442_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37442	Sample	BC37442	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB30	COI	45	BC37442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB30	GAAGGCCTTCCGATGATC	151.77097
BC37442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89074	WGS84	BC37442	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37442_Upin_Bot2011_COI	NEWB30	1	BC37442_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37442	Sample	BC37442	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB30	COI	45	BC37442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB30	CTTTAATTACAGCGTTTTTATTGTTGT	151.77097
BC37443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89124	WGS84	BC37443	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.643	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37443_Mgig_Bot2012_COI	NEWB29	1	BC37443_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37443	Sample	BC37443	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB29	COI	45	BC37443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB29	TCTTATTCGTTGGAGACTTTATAACCCT	151.76864
BC37444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89102	WGS84	BC37444	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37444_Cmae_Bot2011_COI	NEWB28	1	BC37444_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37444	Sample	BC37444	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB28	COI	45	BC37444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB28	ATGAACAGTCTATCCTCCTTTAG	151.76518
BC37444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89102	WGS84	BC37444	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37444_Msal_Bot2012_COI	NEWB28	1	BC37444_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37444	Sample	BC37444	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB28	COI	45	BC37444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB28	GYTAGTTCCRATGATGTTAGCTG	151.76518
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37446_Aamu_Bax2006_COI	NEWB26	1	BC37446_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	GCACAACCGGGATCTTTACTTC	151.77011
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37446_Pcan_Bot2011_IGS	NEWB26	1	BC37446_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB26	IGS	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	CGTAATCCTCAGTACTGGCTA	151.77011
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37446_Sspa_Oph2007_COI	NEWB26	1	BC37446_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	GAAGGCCTTCCGATGATC	151.77011
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37446_Upin_Bot2011_COI	NEWB26	1	BC37446_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	CTTTAATTACAGCGTTTTTATTGTTGT	151.77011
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37446_Vgib_Wil2025_COI	NEWB26	1	BC37446_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	CCCCTCTTTACAAGTTCTTTAGGGT	151.77011
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37447_Asen_Wil2025_COI	NEWB25	1	BC37447_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	GTTGTTGTTACAACACATGCTCTTTTG	151.76678
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.089	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37447_Mgig_Bot2012_COI	NEWB25	1	BC37447_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	TCTTATTCGTTGGAGACTTTATAACCCT	151.76678
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37448_Cmae_Bot2011_COI	NEWB24	1	BC37448_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	ATGAACAGTCTATCCTCCTTTAG	151.77132
BC37448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89382	WGS84	BC37448	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37448_Msal_Bot2012_COI	NEWB24	1	BC37448_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37448	Sample	BC37448	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB24	COI	45	BC37448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB24	GYTAGTTCCRATGATGTTAGCTG	151.77132
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37450_Asen_Wil2025_COI	NEWB22	1	BC37450_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	GTTGTTGTTACAACACATGCTCTTTTG	151.77257
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	41.489	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37450_Mgig_Bot2012_COI	NEWB22	1	BC37450_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	TCTTATTCGTTGGAGACTTTATAACCCT	151.77257
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37451_Cmae_Bot2011_COI	NEWB21	1	BC37451_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	ATGAACAGTCTATCCTCCTTTAG	151.76998
BC37451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89642	WGS84	BC37451	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37451_Msal_Bot2012_COI	NEWB21	1	BC37451_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37451	Sample	BC37451	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB21	COI	45	BC37451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB21	GYTAGTTCCRATGATGTTAGCTG	151.76998
BC37453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91353	WGS84	BC37453	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37453_Aamu_Bax2006_COI	NEWB19	1	BC37453_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37453	Sample	BC37453	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB19	COI	45	BC37453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB19	GCACAACCGGGATCTTTACTTC	151.77278
BC37453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91353	WGS84	BC37453	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37453_Pcan_Bot2011_IGS	NEWB19	1	BC37453_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37453	Sample	BC37453	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB19	IGS	45	BC37453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB19	CGTAATCCTCAGTACTGGCTA	151.77278
BC37453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91353	WGS84	BC37453	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37453_Sspa_Oph2007_COI	NEWB19	1	BC37453_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37453	Sample	BC37453	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB19	COI	45	BC37453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB19	GAAGGCCTTCCGATGATC	151.77278
BC37453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91353	WGS84	BC37453	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37453_Upin_Bot2011_COI	NEWB19	1	BC37453_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37453	Sample	BC37453	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB19	COI	45	BC37453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB19	CTTTAATTACAGCGTTTTTATTGTTGT	151.77278
BC37454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91478	WGS84	BC37454	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37454_Mgig_Bot2012_COI	NEWB18	1	BC37454_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37454	Sample	BC37454	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB18	COI	45	BC37454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB18	TCTTATTCGTTGGAGACTTTATAACCCT	151.77479
BC37455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91641	WGS84	BC37455	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37455_Cmae_Bot2011_COI	NEWB17	1	BC37455_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37455	Sample	BC37455	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB17	COI	45	BC37455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB17	ATGAACAGTCTATCCTCCTTTAG	151.77645
BC37455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91641	WGS84	BC37455	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37455_Msal_Bot2012_COI	NEWB17	1	BC37455_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37455	Sample	BC37455	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB17	COI	45	BC37455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB17	GYTAGTTCCRATGATGTTAGCTG	151.77645
BC37457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91821	WGS84	BC37457	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37457_Aamu_Bax2006_COI	NEWB15	1	BC37457_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37457	Sample	BC37457	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB15	COI	45	BC37457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB15	GCACAACCGGGATCTTTACTTC	151.77566
BC37457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91821	WGS84	BC37457	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37457_Pcan_Bot2011_IGS	NEWB15	1	BC37457_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37457	Sample	BC37457	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB15	IGS	45	BC37457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB15	CGTAATCCTCAGTACTGGCTA	151.77566
BC37457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91821	WGS84	BC37457	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37457_Sspa_Oph2007_COI	NEWB15	1	BC37457_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37457	Sample	BC37457	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB15	COI	45	BC37457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB15	GAAGGCCTTCCGATGATC	151.77566
BC37457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91821	WGS84	BC37457	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37457_Upin_Bot2011_COI	NEWB15	1	BC37457_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37457	Sample	BC37457	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB15	COI	45	BC37457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB15	CTTTAATTACAGCGTTTTTATTGTTGT	151.77566
BC37458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91994	WGS84	BC37458	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.16	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37458_Mgig_Bot2012_COI	NEWB14	1	BC37458_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37458	Sample	BC37458	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB14	COI	45	BC37458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB14	TCTTATTCGTTGGAGACTTTATAACCCT	151.77723
BC37459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89868	WGS84	BC37459	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37459_Cmae_Bot2011_COI	NEWB13	1	BC37459_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37459	Sample	BC37459	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB13	COI	45	BC37459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB13	ATGAACAGTCTATCCTCCTTTAG	151.77045
BC37459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89868	WGS84	BC37459	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37459_Msal_Bot2012_COI	NEWB13	1	BC37459_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37459	Sample	BC37459	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB13	COI	45	BC37459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB13	GYTAGTTCCRATGATGTTAGCTG	151.77045
BC37460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90041	WGS84	BC37460	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37460_Aamu_Bax2006_COI	NEWB12	1	BC37460_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37460	Sample	BC37460	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB12	COI	45	BC37460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB12	GCACAACCGGGATCTTTACTTC	151.77233
BC37460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90041	WGS84	BC37460	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37460_Pcan_Bot2011_IGS	NEWB12	1	BC37460_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37460	Sample	BC37460	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB12	IGS	45	BC37460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB12	CGTAATCCTCAGTACTGGCTA	151.77233
BC37460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90041	WGS84	BC37460	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37460_Sspa_Oph2007_COI	NEWB12	1	BC37460_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37460	Sample	BC37460	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB12	COI	45	BC37460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB12	GAAGGCCTTCCGATGATC	151.77233
BC37460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90041	WGS84	BC37460	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37460_Upin_Bot2011_COI	NEWB12	1	BC37460_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37460	Sample	BC37460	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB12	COI	45	BC37460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB12	CTTTAATTACAGCGTTTTTATTGTTGT	151.77233
BC37461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90277	WGS84	BC37461	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.218	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37461_Mgig_Bot2012_COI	NEWB11	1	BC37461_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37461	Sample	BC37461	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB11	COI	45	BC37461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB11	TCTTATTCGTTGGAGACTTTATAACCCT	151.77329
BC37462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90186	WGS84	BC37462	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37462_Cmae_Bot2011_COI	NEWB10	1	BC37462_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37462	Sample	BC37462	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB10	COI	45	BC37462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB10	ATGAACAGTCTATCCTCCTTTAG	151.77113
BC37462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90186	WGS84	BC37462	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37462_Msal_Bot2012_COI	NEWB10	1	BC37462_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37462	Sample	BC37462	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB10	COI	45	BC37462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB10	GYTAGTTCCRATGATGTTAGCTG	151.77113
BC37464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90649	WGS84	BC37464	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37464_Aamu_Bax2006_COI	NEWB8	1	BC37464_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37464	Sample	BC37464	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB8	COI	45	BC37464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB8	GCACAACCGGGATCTTTACTTC	151.77151
BC37464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90649	WGS84	BC37464	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37464_Pcan_Bot2011_IGS	NEWB8	1	BC37464_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37464	Sample	BC37464	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB8	IGS	45	BC37464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB8	CGTAATCCTCAGTACTGGCTA	151.77151
BC37464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90649	WGS84	BC37464	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37464_Sspa_Oph2007_COI	NEWB8	1	BC37464_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37464	Sample	BC37464	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB8	COI	45	BC37464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB8	GAAGGCCTTCCGATGATC	151.77151
BC37464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90649	WGS84	BC37464	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37464_Upin_Bot2011_COI	NEWB8	1	BC37464_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37464	Sample	BC37464	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB8	COI	45	BC37464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB8	CTTTAATTACAGCGTTTTTATTGTTGT	151.77151
BC37465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91934	WGS84	BC37465	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37465_Mgig_Bot2012_COI	NEWB7	1	BC37465_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37465	Sample	BC37465	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB7	COI	45	BC37465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB7	TCTTATTCGTTGGAGACTTTATAACCCT	151.76933
BC37466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92197	WGS84	BC37466	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37466_Cmae_Bot2011_COI	NEWB6	1	BC37466_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37466	Sample	BC37466	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB6	COI	45	BC37466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB6	ATGAACAGTCTATCCTCCTTTAG	151.77168
BC37466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92197	WGS84	BC37466	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37466_Msal_Bot2012_COI	NEWB6	1	BC37466_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37466	Sample	BC37466	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB6	COI	45	BC37466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB6	GYTAGTTCCRATGATGTTAGCTG	151.77168
BC37468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92383	WGS84	BC37468	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37468_Aamu_Bax2006_COI	NEWB4	1	BC37468_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37468	Sample	BC37468	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB4	COI	45	BC37468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB4	GCACAACCGGGATCTTTACTTC	151.77345
BC37468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92383	WGS84	BC37468	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37468_Pcan_Bot2011_IGS	NEWB4	1	BC37468_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37468	Sample	BC37468	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB4	IGS	45	BC37468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB4	CGTAATCCTCAGTACTGGCTA	151.77345
BC37468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92383	WGS84	BC37468	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37468_Sspa_Oph2007_COI	NEWB4	1	BC37468_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37468	Sample	BC37468	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB4	COI	45	BC37468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB4	GAAGGCCTTCCGATGATC	151.77345
BC37468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92383	WGS84	BC37468	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37468_Upin_Bot2011_COI	NEWB4	1	BC37468_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37468	Sample	BC37468	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB4	COI	45	BC37468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB4	CTTTAATTACAGCGTTTTTATTGTTGT	151.77345
BC37469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92421	WGS84	BC37469	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.236	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37469_Mgig_Bot2012_COI	NEWB3	1	BC37469_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37469	Sample	BC37469	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB3	COI	45	BC37469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB3	TCTTATTCGTTGGAGACTTTATAACCCT	151.77064
BC37471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92394	WGS84	BC37471	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37471_Aamu_Bax2006_COI	NEWB1	1	BC37471_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37471	Sample	BC37471	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB1	COI	45	BC37471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB1	GCACAACCGGGATCTTTACTTC	151.76439
BC37471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92394	WGS84	BC37471	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37471_Pcan_Bot2011_IGS	NEWB1	1	BC37471_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37471	Sample	BC37471	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB1	IGS	45	BC37471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB1	CGTAATCCTCAGTACTGGCTA	151.76439
BC37471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92394	WGS84	BC37471	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37471_Sspa_Oph2007_COI	NEWB1	1	BC37471_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37471	Sample	BC37471	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB1	COI	45	BC37471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB1	GAAGGCCTTCCGATGATC	151.76439
BC37471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92394	WGS84	BC37471	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37471_Upin_Bot2011_COI	NEWB1	1	BC37471_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37471	Sample	BC37471	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB1	COI	45	BC37471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB1	CTTTAATTACAGCGTTTTTATTGTTGT	151.76439
BC37472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00231	WGS84	BC37472	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37472_Mgig_Bot2012_COI	BOTB1	1	BC37472_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37472	Sample	BC37472	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB1	COI	45	BC37472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB1	TCTTATTCGTTGGAGACTTTATAACCCT	151.20921
BC37473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00212	WGS84	BC37473	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37473_Cmae_Bot2011_COI	BOTB2	1	BC37473_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37473	Sample	BC37473	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB2	COI	45	BC37473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB2	ATGAACAGTCTATCCTCCTTTAG	151.21116
BC37473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00212	WGS84	BC37473	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37473_Msal_Bot2012_COI	BOTB2	1	BC37473_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37473	Sample	BC37473	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB2	COI	45	BC37473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB2	GYTAGTTCCRATGATGTTAGCTG	151.21116
BC37475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99948	WGS84	BC37475	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37475_Aamu_Bax2006_COI	BOTB4	1	BC37475_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37475	Sample	BC37475	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB4	COI	45	BC37475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB4	GCACAACCGGGATCTTTACTTC	151.21169
BC37475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99948	WGS84	BC37475	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37475_Pcan_Bot2011_IGS	BOTB4	1	BC37475_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37475	Sample	BC37475	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB4	IGS	45	BC37475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB4	CGTAATCCTCAGTACTGGCTA	151.21169
BC37475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99948	WGS84	BC37475	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37475_Sspa_Oph2007_COI	BOTB4	1	BC37475_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37475	Sample	BC37475	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB4	COI	45	BC37475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB4	GAAGGCCTTCCGATGATC	151.21169
BC37475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99948	WGS84	BC37475	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37475_Upin_Bot2011_COI	BOTB4	1	BC37475_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37475	Sample	BC37475	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB4	COI	45	BC37475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB4	CTTTAATTACAGCGTTTTTATTGTTGT	151.21169
BC37476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99864	WGS84	BC37476	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37476_Mgig_Bot2012_COI	BOTB5	1	BC37476_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37476	Sample	BC37476	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB5	COI	45	BC37476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB5	TCTTATTCGTTGGAGACTTTATAACCCT	151.20885
BC37477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45332	WGS84	BC37477	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37477_Cmae_Bot2011_COI	KEMB35	1	BC37477_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37477	Sample	BC37477	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB35	COI	45	BC37477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB35	ATGAACAGTCTATCCTCCTTTAG	150.89541
BC37477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45332	WGS84	BC37477	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37477_Msal_Bot2012_COI	KEMB35	1	BC37477_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37477	Sample	BC37477	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB35	COI	45	BC37477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB35	GYTAGTTCCRATGATGTTAGCTG	150.89541
BC37479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45485	WGS84	BC37479	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37479_Aamu_Bax2006_COI	KEMB33	1	BC37479_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37479	Sample	BC37479	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB33	COI	45	BC37479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB33	GCACAACCGGGATCTTTACTTC	150.8876
BC37479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45485	WGS84	BC37479	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37479_Pcan_Bot2011_IGS	KEMB33	1	BC37479_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37479	Sample	BC37479	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB33	IGS	45	BC37479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB33	CGTAATCCTCAGTACTGGCTA	150.8876
BC37479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45485	WGS84	BC37479	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37479_Sspa_Oph2007_COI	KEMB33	1	BC37479_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37479	Sample	BC37479	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB33	COI	45	BC37479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB33	GAAGGCCTTCCGATGATC	150.8876
BC37479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45485	WGS84	BC37479	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37479_Upin_Bot2011_COI	KEMB33	1	BC37479_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37479	Sample	BC37479	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB33	COI	45	BC37479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB33	CTTTAATTACAGCGTTTTTATTGTTGT	150.8876
BC37480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45578	WGS84	BC37480	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37480_Cmae_Bot2011_COI	KEMB32	1	BC37480_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37480	Sample	BC37480	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB32	COI	45	BC37480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB32	ATGAACAGTCTATCCTCCTTTAG	150.89543
BC37480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45578	WGS84	BC37480	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37480_Msal_Bot2012_COI	KEMB32	1	BC37480_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37480	Sample	BC37480	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB32	COI	45	BC37480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB32	GYTAGTTCCRATGATGTTAGCTG	150.89543
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37482_Aamu_Bax2006_COI	KEMB30	1	BC37482_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	GCACAACCGGGATCTTTACTTC	150.88835
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37482_Pcan_Bot2011_IGS	KEMB30	1	BC37482_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB30	IGS	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	CGTAATCCTCAGTACTGGCTA	150.88835
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37482_Sspa_Oph2007_COI	KEMB30	1	BC37482_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	GAAGGCCTTCCGATGATC	150.88835
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37482_Upin_Bot2011_COI	KEMB30	1	BC37482_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	CTTTAATTACAGCGTTTTTATTGTTGT	150.88835
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37482_Vgib_Wil2025_COI	KEMB30	1	BC37482_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	CCCCTCTTTACAAGTTCTTTAGGGT	150.88835
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37483_Asen_Wil2025_COI	KEMB29	1	BC37483_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	GTTGTTGTTACAACACATGCTCTTTTG	150.89545
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.575	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37483_Mgig_Bot2012_COI	KEMB29	1	BC37483_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	TCTTATTCGTTGGAGACTTTATAACCCT	150.89545
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37484_Cmae_Bot2011_COI	KEMB28	1	BC37484_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	ATGAACAGTCTATCCTCCTTTAG	150.89275
BC37484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45855	WGS84	BC37484	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37484_Msal_Bot2012_COI	KEMB28	1	BC37484_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37484	Sample	BC37484	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB28	COI	45	BC37484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB28	GYTAGTTCCRATGATGTTAGCTG	150.89275
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37486_Aamu_Bax2006_COI	KEMB26	1	BC37486_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	GCACAACCGGGATCTTTACTTC	150.89653
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37486_Pcan_Bot2011_IGS	KEMB26	1	BC37486_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB26	IGS	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	CGTAATCCTCAGTACTGGCTA	150.89653
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37486_Sspa_Oph2007_COI	KEMB26	1	BC37486_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	GAAGGCCTTCCGATGATC	150.89653
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37486_Upin_Bot2011_COI	KEMB26	1	BC37486_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	CTTTAATTACAGCGTTTTTATTGTTGT	150.89653
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37486_Vgib_Wil2025_COI	KEMB26	1	BC37486_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	CCCCTCTTTACAAGTTCTTTAGGGT	150.89653
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37487_Asen_Wil2025_COI	KEMB25	1	BC37487_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	GTTGTTGTTACAACACATGCTCTTTTG	150.89433
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37487_Mgig_Bot2012_COI	KEMB25	1	BC37487_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	TCTTATTCGTTGGAGACTTTATAACCCT	150.89433
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37488_Cmae_Bot2011_COI	KEMB24	1	BC37488_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	ATGAACAGTCTATCCTCCTTTAG	150.89227
BC37488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46019	WGS84	BC37488	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37488_Msal_Bot2012_COI	KEMB24	1	BC37488_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37488	Sample	BC37488	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB24	COI	45	BC37488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB24	GYTAGTTCCRATGATGTTAGCTG	150.89227
BC37490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46159	WGS84	BC37490	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37490_Mgig_Bot2012_COI	KEMB22	1	BC37490_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37490	Sample	BC37490	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB22	COI	45	BC37490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB22	TCTTATTCGTTGGAGACTTTATAACCCT	150.89548
BC37491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46158	WGS84	BC37491	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37491_Cmae_Bot2011_COI	KEMB21	1	BC37491_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37491	Sample	BC37491	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB21	COI	45	BC37491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB21	ATGAACAGTCTATCCTCCTTTAG	150.89381
BC37491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46158	WGS84	BC37491	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37491_Msal_Bot2012_COI	KEMB21	1	BC37491_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37491	Sample	BC37491	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB21	COI	45	BC37491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB21	GYTAGTTCCRATGATGTTAGCTG	150.89381
BC37493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46383	WGS84	BC37493	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37493_Aamu_Bax2006_COI	KEMB19	1	BC37493_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37493	Sample	BC37493	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB19	COI	45	BC37493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB19	GCACAACCGGGATCTTTACTTC	150.90512
BC37493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46383	WGS84	BC37493	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37493_Pcan_Bot2011_IGS	KEMB19	1	BC37493_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37493	Sample	BC37493	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB19	IGS	45	BC37493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB19	CGTAATCCTCAGTACTGGCTA	150.90512
BC37493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46383	WGS84	BC37493	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37493_Sspa_Oph2007_COI	KEMB19	1	BC37493_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37493	Sample	BC37493	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB19	COI	45	BC37493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB19	GAAGGCCTTCCGATGATC	150.90512
BC37493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46383	WGS84	BC37493	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37493_Upin_Bot2011_COI	KEMB19	1	BC37493_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37493	Sample	BC37493	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB19	COI	45	BC37493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB19	CTTTAATTACAGCGTTTTTATTGTTGT	150.90512
BC37494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46358	WGS84	BC37494	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37494_Mgig_Bot2012_COI	KEMB18	1	BC37494_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37494	Sample	BC37494	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB18	COI	45	BC37494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB18	TCTTATTCGTTGGAGACTTTATAACCCT	150.90353
BC37495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46364	WGS84	BC37495	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37495_Cmae_Bot2011_COI	KEMB17	1	BC37495_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37495	Sample	BC37495	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB17	COI	45	BC37495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB17	ATGAACAGTCTATCCTCCTTTAG	150.90002
BC37495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46364	WGS84	BC37495	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37495_Msal_Bot2012_COI	KEMB17	1	BC37495_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37495	Sample	BC37495	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB17	COI	45	BC37495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB17	GYTAGTTCCRATGATGTTAGCTG	150.90002
BC37497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46504	WGS84	BC37497	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37497_Aamu_Bax2006_COI	KEMB15	1	BC37497_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37497	Sample	BC37497	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB15	COI	45	BC37497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB15	GCACAACCGGGATCTTTACTTC	150.90708
BC37497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46504	WGS84	BC37497	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37497_Pcan_Bot2011_IGS	KEMB15	1	BC37497_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37497	Sample	BC37497	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB15	IGS	45	BC37497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB15	CGTAATCCTCAGTACTGGCTA	150.90708
BC37497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46504	WGS84	BC37497	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37497_Sspa_Oph2007_COI	KEMB15	1	BC37497_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37497	Sample	BC37497	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB15	COI	45	BC37497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB15	GAAGGCCTTCCGATGATC	150.90708
BC37497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46504	WGS84	BC37497	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37497_Upin_Bot2011_COI	KEMB15	1	BC37497_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37497	Sample	BC37497	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB15	COI	45	BC37497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB15	CTTTAATTACAGCGTTTTTATTGTTGT	150.90708
BC37498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46521	WGS84	BC37498	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37498_Mgig_Bot2012_COI	KEMB14	1	BC37498_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37498	Sample	BC37498	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB14	COI	45	BC37498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB14	TCTTATTCGTTGGAGACTTTATAACCCT	150.90487
BC37499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46502	WGS84	BC37499	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37499_Cmae_Bot2011_COI	KEMB13	1	BC37499_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37499	Sample	BC37499	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB13	COI	45	BC37499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB13	ATGAACAGTCTATCCTCCTTTAG	150.90137
BC37499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46502	WGS84	BC37499	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37499_Msal_Bot2012_COI	KEMB13	1	BC37499_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37499	Sample	BC37499	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB13	COI	45	BC37499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB13	GYTAGTTCCRATGATGTTAGCTG	150.90137
BC37501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46709	WGS84	BC37501	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37501_Aamu_Bax2006_COI	KEMB11	1	BC37501_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37501	Sample	BC37501	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB11	COI	45	BC37501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB11	GCACAACCGGGATCTTTACTTC	150.90581
BC37501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46709	WGS84	BC37501	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37501_Pcan_Bot2011_IGS	KEMB11	1	BC37501_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37501	Sample	BC37501	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB11	IGS	45	BC37501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB11	CGTAATCCTCAGTACTGGCTA	150.90581
BC37501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46709	WGS84	BC37501	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37501_Sspa_Oph2007_COI	KEMB11	1	BC37501_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37501	Sample	BC37501	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB11	COI	45	BC37501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB11	GAAGGCCTTCCGATGATC	150.90581
BC37501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46709	WGS84	BC37501	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37501_Upin_Bot2011_COI	KEMB11	1	BC37501_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37501	Sample	BC37501	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB11	COI	45	BC37501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB11	CTTTAATTACAGCGTTTTTATTGTTGT	150.90581
BC37502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46689	WGS84	BC37502	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37502_Mgig_Bot2012_COI	KEMB10	1	BC37502_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37502	Sample	BC37502	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB10	COI	45	BC37502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB10	TCTTATTCGTTGGAGACTTTATAACCCT	150.90398
BC37503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46663	WGS84	BC37503	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37503_Cmae_Bot2011_COI	KEMB9	1	BC37503_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37503	Sample	BC37503	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB9	COI	45	BC37503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB9	ATGAACAGTCTATCCTCCTTTAG	150.90134
BC37503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46663	WGS84	BC37503	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37503_Msal_Bot2012_COI	KEMB9	1	BC37503_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37503	Sample	BC37503	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB9	COI	45	BC37503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB9	GYTAGTTCCRATGATGTTAGCTG	150.90134
BC37505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46901	WGS84	BC37505	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37505_Aamu_Bax2006_COI	KEMB7	1	BC37505_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37505	Sample	BC37505	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB7	COI	45	BC37505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB7	GCACAACCGGGATCTTTACTTC	150.90504
BC37505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46901	WGS84	BC37505	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37505_Pcan_Bot2011_IGS	KEMB7	1	BC37505_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37505	Sample	BC37505	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB7	IGS	45	BC37505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB7	CGTAATCCTCAGTACTGGCTA	150.90504
BC37505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46901	WGS84	BC37505	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37505_Sspa_Oph2007_COI	KEMB7	1	BC37505_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37505	Sample	BC37505	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB7	COI	45	BC37505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB7	GAAGGCCTTCCGATGATC	150.90504
BC37505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46901	WGS84	BC37505	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37505_Upin_Bot2011_COI	KEMB7	1	BC37505_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37505	Sample	BC37505	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB7	COI	45	BC37505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB7	CTTTAATTACAGCGTTTTTATTGTTGT	150.90504
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37506_Asen_Wil2025_COI	KEMB6	1	BC37506_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	GTTGTTGTTACAACACATGCTCTTTTG	150.90624
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37506_Mgig_Bot2012_COI	KEMB6	1	BC37506_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	TCTTATTCGTTGGAGACTTTATAACCCT	150.90624
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63715_Aamu_Bax2006_COI	FREB30	1	BC63715_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	GCACAACCGGGATCTTTACTTC	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63715_Pcan_Bot2011_IGS	FREB30	1	BC63715_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB30	IGS	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	CGTAATCCTCAGTACTGGCTA	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63715_Pper_Dia2013_COI	FREB30	1	BC63715_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Pper_Dia2013_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	CTTAGTGGCATTAATTCGDAATCC	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63715_Sspa_Oph2007_COI	FREB30	1	BC63715_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	GAAGGCCTTCCGATGATC	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63715_Upin_Bot2011_COI	FREB30	1	BC63715_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Upin_Bot2011_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	CTTTAATTACAGCGTTTTTATTGTTGT	115.74996
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63716_Cjap_Sim2018_COI	FREB25	1	BC63716_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63716_Mgig_Bot2012_COI	FREB25	1	BC63716_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	TCTTATTCGTTGGAGACTTTATAACCCT	115.74773
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63717_Cmae_Bot2011_COI	FREB20	1	BC63717_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	ATGAACAGTCTATCCTCCTTTAG	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63717_Msal_Bot2012_COI	FREB20	1	BC63717_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Msal_Bot2012_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	GYTAGTTCCRATGATGTTAGCTG	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63717_Pvir_Dia2013_COI	FREB20	1	BC63717_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	CTTAGTGGCATTAATTCGDAATCC	115.74722
BC63717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04561	WGS84	BC63717	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63717_Rhar_Sim2018_COI	FREB20	1	BC63717_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63717	Sample	BC63717	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB20	COI	45	BC63717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB20	CTGCTGCTATTGCTCATG	115.74722
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63719_Aamu_Bax2006_COI	FREB10	1	BC63719_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	GCACAACCGGGATCTTTACTTC	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63719_Pcan_Bot2011_IGS	FREB10	1	BC63719_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB10	IGS	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	CGTAATCCTCAGTACTGGCTA	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63719_Pper_Dia2013_COI	FREB10	1	BC63719_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Pper_Dia2013_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	CTTAGTGGCATTAATTCGDAATCC	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63719_Sspa_Oph2007_COI	FREB10	1	BC63719_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	GAAGGCCTTCCGATGATC	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63719_Upin_Bot2011_COI	FREB10	1	BC63719_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Upin_Bot2011_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	CTTTAATTACAGCGTTTTTATTGTTGT	115.74163
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63720_Cmae_Bot2011_COI	FREB5	1	BC63720_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	ATGAACAGTCTATCCTCCTTTAG	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63720_Msal_Bot2012_COI	FREB5	1	BC63720_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Msal_Bot2012_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	GYTAGTTCCRATGATGTTAGCTG	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63720_Pvir_Dia2013_COI	FREB5	1	BC63720_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	CTTAGTGGCATTAATTCGDAATCC	115.74062
BC63720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.051	WGS84	BC63720	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63720_Rhar_Sim2018_COI	FREB5	1	BC63720_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63720	Sample	BC63720	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB5	COI	45	BC63720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB5	CTGCTGCTATTGCTCATG	115.74062
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63722_Aamu_Bax2006_COI	FREB32	1	BC63722_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	GCACAACCGGGATCTTTACTTC	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63722_Pcan_Bot2011_IGS	FREB32	1	BC63722_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB32	IGS	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	CGTAATCCTCAGTACTGGCTA	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63722_Pper_Dia2013_COI	FREB32	1	BC63722_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Pper_Dia2013_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	CTTAGTGGCATTAATTCGDAATCC	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63722_Sspa_Oph2007_COI	FREB32	1	BC63722_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	GAAGGCCTTCCGATGATC	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63722_Upin_Bot2011_COI	FREB32	1	BC63722_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Upin_Bot2011_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	CTTTAATTACAGCGTTTTTATTGTTGT	115.7509
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63723_Cjap_Sim2018_COI	FREB31	1	BC63723_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63723_Mgig_Bot2012_COI	FREB31	1	BC63723_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	TCTTATTCGTTGGAGACTTTATAACCCT	115.75126
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63724_Cmae_Bot2011_COI	FREB29	1	BC63724_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	ATGAACAGTCTATCCTCCTTTAG	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63724_Msal_Bot2012_COI	FREB29	1	BC63724_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Msal_Bot2012_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	GYTAGTTCCRATGATGTTAGCTG	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63724_Pvir_Dia2013_COI	FREB29	1	BC63724_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	CTTAGTGGCATTAATTCGDAATCC	115.7501
BC63724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04225	WGS84	BC63724	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63724_Rhar_Sim2018_COI	FREB29	1	BC63724_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63724	Sample	BC63724	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB29	COI	45	BC63724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB29	CTGCTGCTATTGCTCATG	115.7501
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63726_Aamu_Bax2006_COI	FREB27	1	BC63726_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	GCACAACCGGGATCTTTACTTC	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63726_Pcan_Bot2011_IGS	FREB27	1	BC63726_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB27	IGS	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	CGTAATCCTCAGTACTGGCTA	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63726_Pper_Dia2013_COI	FREB27	1	BC63726_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Pper_Dia2013_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	CTTAGTGGCATTAATTCGDAATCC	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63726_Sspa_Oph2007_COI	FREB27	1	BC63726_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	GAAGGCCTTCCGATGATC	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63726_Upin_Bot2011_COI	FREB27	1	BC63726_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Upin_Bot2011_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	CTTTAATTACAGCGTTTTTATTGTTGT	115.74747
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63727_Cjap_Sim2018_COI	FREB26	1	BC63727_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63727_Mgig_Bot2012_COI	FREB26	1	BC63727_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	TCTTATTCGTTGGAGACTTTATAACCCT	115.74931
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63728_Cmae_Bot2011_COI	FREB24	1	BC63728_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	ATGAACAGTCTATCCTCCTTTAG	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63728_Msal_Bot2012_COI	FREB24	1	BC63728_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Msal_Bot2012_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	GYTAGTTCCRATGATGTTAGCTG	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63728_Pvir_Dia2013_COI	FREB24	1	BC63728_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	CTTAGTGGCATTAATTCGDAATCC	115.7464
BC63728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04372	WGS84	BC63728	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63728_Rhar_Sim2018_COI	FREB24	1	BC63728_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63728	Sample	BC63728	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB24	COI	45	BC63728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB24	CTGCTGCTATTGCTCATG	115.7464
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63730_Cjap_Sim2018_COI	FREB22	1	BC63730_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63730_Mgig_Bot2012_COI	FREB22	1	BC63730_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	TCTTATTCGTTGGAGACTTTATAACCCT	115.74684
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63731_Cmae_Bot2011_COI	FREB21	1	BC63731_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	ATGAACAGTCTATCCTCCTTTAG	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63731_Msal_Bot2012_COI	FREB21	1	BC63731_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Msal_Bot2012_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	GYTAGTTCCRATGATGTTAGCTG	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63731_Pvir_Dia2013_COI	FREB21	1	BC63731_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	CTTAGTGGCATTAATTCGDAATCC	115.74527
BC63731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04449	WGS84	BC63731	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63731_Rhar_Sim2018_COI	FREB21	1	BC63731_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63731	Sample	BC63731	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB21	COI	45	BC63731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB21	CTGCTGCTATTGCTCATG	115.74527
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63733_Aamu_Bax2006_COI	FREB18	1	BC63733_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	GCACAACCGGGATCTTTACTTC	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63733_Pcan_Bot2011_IGS	FREB18	1	BC63733_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB18	IGS	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	CGTAATCCTCAGTACTGGCTA	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63733_Pper_Dia2013_COI	FREB18	1	BC63733_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Pper_Dia2013_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	CTTAGTGGCATTAATTCGDAATCC	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63733_Sspa_Oph2007_COI	FREB18	1	BC63733_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	GAAGGCCTTCCGATGATC	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63733_Upin_Bot2011_COI	FREB18	1	BC63733_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Upin_Bot2011_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	CTTTAATTACAGCGTTTTTATTGTTGT	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04584	WGS84	BC63733	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63733_Vgib_Wil2025_COI	FREB18	1	BC63733_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	CCCCTCTTTACAAGTTCTTTAGGGT	115.74461
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04686	WGS84	BC63734	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	23.667	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63734_Asen_Wil2025_COI	FREB17	1	BC63734_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Present	Sample	Asen_Wil2025_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	GTTGTTGTTACAACACATGCTCTTTTG	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63734_Cjap_Sim2018_COI	FREB17	1	BC63734_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63734_Mgig_Bot2012_COI	FREB17	1	BC63734_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	TCTTATTCGTTGGAGACTTTATAACCCT	115.74626
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63735_Cmae_Bot2011_COI	FREB16	1	BC63735_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	ATGAACAGTCTATCCTCCTTTAG	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63735_Msal_Bot2012_COI	FREB16	1	BC63735_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Msal_Bot2012_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	GYTAGTTCCRATGATGTTAGCTG	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63735_Pvir_Dia2013_COI	FREB16	1	BC63735_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	CTTAGTGGCATTAATTCGDAATCC	115.7444
BC63735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04642	WGS84	BC63735	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63735_Rhar_Sim2018_COI	FREB16	1	BC63735_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63735	Sample	BC63735	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB16	COI	45	BC63735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB16	CTGCTGCTATTGCTCATG	115.7444
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63737_Aamu_Bax2006_COI	FREB13	1	BC63737_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	GCACAACCGGGATCTTTACTTC	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63737_Pcan_Bot2011_IGS	FREB13	1	BC63737_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB13	IGS	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	CGTAATCCTCAGTACTGGCTA	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63737_Pper_Dia2013_COI	FREB13	1	BC63737_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Pper_Dia2013_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	CTTAGTGGCATTAATTCGDAATCC	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63737_Sspa_Oph2007_COI	FREB13	1	BC63737_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	GAAGGCCTTCCGATGATC	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63737_Upin_Bot2011_COI	FREB13	1	BC63737_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Upin_Bot2011_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	CTTTAATTACAGCGTTTTTATTGTTGT	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04791	WGS84	BC63737	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63737_Vgib_Wil2025_COI	FREB13	1	BC63737_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	CCCCTCTTTACAAGTTCTTTAGGGT	115.74378
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04822	WGS84	BC63738	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	24.549	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63738_Asen_Wil2025_COI	FREB12	1	BC63738_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Present	Sample	Asen_Wil2025_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	GTTGTTGTTACAACACATGCTCTTTTG	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63738_Cjap_Sim2018_COI	FREB12	1	BC63738_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63738_Mgig_Bot2012_COI	FREB12	1	BC63738_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	TCTTATTCGTTGGAGACTTTATAACCCT	115.74316
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63739_Cmae_Bot2011_COI	FREB11	1	BC63739_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	ATGAACAGTCTATCCTCCTTTAG	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63739_Msal_Bot2012_COI	FREB11	1	BC63739_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Msal_Bot2012_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	GYTAGTTCCRATGATGTTAGCTG	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63739_Pvir_Dia2013_COI	FREB11	1	BC63739_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	CTTAGTGGCATTAATTCGDAATCC	115.74423
BC63739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04898	WGS84	BC63739	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63739_Rhar_Sim2018_COI	FREB11	1	BC63739_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63739	Sample	BC63739	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB11	COI	45	BC63739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB11	CTGCTGCTATTGCTCATG	115.74423
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63740_Aamu_Bax2006_COI	FREB9	1	BC63740_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	GCACAACCGGGATCTTTACTTC	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63740_Pcan_Bot2011_IGS	FREB9	1	BC63740_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB9	IGS	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	CGTAATCCTCAGTACTGGCTA	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63740_Pper_Dia2013_COI	FREB9	1	BC63740_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Pper_Dia2013_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	CTTAGTGGCATTAATTCGDAATCC	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63740_Sspa_Oph2007_COI	FREB9	1	BC63740_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	GAAGGCCTTCCGATGATC	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63740_Upin_Bot2011_COI	FREB9	1	BC63740_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Upin_Bot2011_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	CTTTAATTACAGCGTTTTTATTGTTGT	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.0496	WGS84	BC63740	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63740_Vgib_Wil2025_COI	FREB9	1	BC63740_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	CCCCTCTTTACAAGTTCTTTAGGGT	115.74163
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05009	WGS84	BC63741	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	28.706	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63741_Asen_Wil2025_COI	FREB8	1	BC63741_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Present	Sample	Asen_Wil2025_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	GTTGTTGTTACAACACATGCTCTTTTG	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63741_Cjap_Sim2018_COI	FREB8	1	BC63741_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63741_Mgig_Bot2012_COI	FREB8	1	BC63741_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	TCTTATTCGTTGGAGACTTTATAACCCT	115.74305
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63742_Cmae_Bot2011_COI	FREB7	1	BC63742_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	ATGAACAGTCTATCCTCCTTTAG	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63742_Msal_Bot2012_COI	FREB7	1	BC63742_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Msal_Bot2012_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	GYTAGTTCCRATGATGTTAGCTG	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63742_Pvir_Dia2013_COI	FREB7	1	BC63742_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	CTTAGTGGCATTAATTCGDAATCC	115.74177
BC63742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63742	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63742_Rhar_Sim2018_COI	FREB7	1	BC63742_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63742	Sample	BC63742	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB7	COI	45	BC63742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB7	CTGCTGCTATTGCTCATG	115.74177
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63744_Aamu_Bax2006_COI	FREB4	1	BC63744_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	GCACAACCGGGATCTTTACTTC	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63744_Pcan_Bot2011_IGS	FREB4	1	BC63744_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB4	IGS	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	CGTAATCCTCAGTACTGGCTA	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63744_Pper_Dia2013_COI	FREB4	1	BC63744_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Pper_Dia2013_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	CTTAGTGGCATTAATTCGDAATCC	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63744_Sspa_Oph2007_COI	FREB4	1	BC63744_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	GAAGGCCTTCCGATGATC	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63744_Upin_Bot2011_COI	FREB4	1	BC63744_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Upin_Bot2011_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	CTTTAATTACAGCGTTTTTATTGTTGT	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05213	WGS84	BC63744	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63744_Vgib_Wil2025_COI	FREB4	1	BC63744_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	CCCCTCTTTACAAGTTCTTTAGGGT	115.74122
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05217	WGS84	BC63745	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	25.345	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63745_Asen_Wil2025_COI	FREB3	1	BC63745_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Present	Sample	Asen_Wil2025_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	GTTGTTGTTACAACACATGCTCTTTTG	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63745_Cjap_Sim2018_COI	FREB3	1	BC63745_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63745_Mgig_Bot2012_COI	FREB3	1	BC63745_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	TCTTATTCGTTGGAGACTTTATAACCCT	115.73963
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63746_Cmae_Bot2011_COI	FREB2	1	BC63746_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	ATGAACAGTCTATCCTCCTTTAG	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63746_Msal_Bot2012_COI	FREB2	1	BC63746_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Msal_Bot2012_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	GYTAGTTCCRATGATGTTAGCTG	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63746_Pvir_Dia2013_COI	FREB2	1	BC63746_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	CTTAGTGGCATTAATTCGDAATCC	115.73868
BC63746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05337	WGS84	BC63746	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63746_Rhar_Sim2018_COI	FREB2	1	BC63746_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63746	Sample	BC63746	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB2	COI	45	BC63746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB2	CTGCTGCTATTGCTCATG	115.73868
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63748_Aamu_Bax2006_COI	KWIB60	1	BC63748_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	GCACAACCGGGATCTTTACTTC	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63748_Pcan_Bot2011_IGS	KWIB60	1	BC63748_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB60	IGS	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	CGTAATCCTCAGTACTGGCTA	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63748_Pper_Dia2013_COI	KWIB60	1	BC63748_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	CTTAGTGGCATTAATTCGDAATCC	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63748_Sspa_Oph2007_COI	KWIB60	1	BC63748_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	GAAGGCCTTCCGATGATC	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63748_Upin_Bot2011_COI	KWIB60	1	BC63748_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	CTTTAATTACAGCGTTTTTATTGTTGT	115.77273
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63749_Cjap_Sim2018_COI	KWIB55	1	BC63749_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63749_Mgig_Bot2012_COI	KWIB55	1	BC63749_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	TCTTATTCGTTGGAGACTTTATAACCCT	115.77353
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63751_Aamu_Bax2006_COI	KWIB45	1	BC63751_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	GCACAACCGGGATCTTTACTTC	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63751_Pcan_Bot2011_IGS	KWIB45	1	BC63751_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB45	IGS	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	CGTAATCCTCAGTACTGGCTA	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63751_Pper_Dia2013_COI	KWIB45	1	BC63751_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	CTTAGTGGCATTAATTCGDAATCC	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63751_Sspa_Oph2007_COI	KWIB45	1	BC63751_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	GAAGGCCTTCCGATGATC	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63751_Upin_Bot2011_COI	KWIB45	1	BC63751_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	CTTTAATTACAGCGTTTTTATTGTTGT	115.76082
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63752_Cjap_Sim2018_COI	KWIB40	1	BC63752_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63752_Mgig_Bot2012_COI	KWIB40	1	BC63752_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	TCTTATTCGTTGGAGACTTTATAACCCT	115.7574
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63753_Cmae_Bot2011_COI	KWIB35	1	BC63753_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	ATGAACAGTCTATCCTCCTTTAG	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63753_Msal_Bot2012_COI	KWIB35	1	BC63753_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	GYTAGTTCCRATGATGTTAGCTG	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63753_Pvir_Dia2013_COI	KWIB35	1	BC63753_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	CTTAGTGGCATTAATTCGDAATCC	115.75715
BC63753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23274	WGS84	BC63753	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63753_Rhar_Sim2018_COI	KWIB35	1	BC63753_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63753	Sample	BC63753	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB35	COI	45	BC63753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB35	CTGCTGCTATTGCTCATG	115.75715
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63755_Aamu_Bax2006_COI	KWIB25	1	BC63755_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	GCACAACCGGGATCTTTACTTC	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63755_Pcan_Bot2011_IGS	KWIB25	1	BC63755_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB25	IGS	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	CGTAATCCTCAGTACTGGCTA	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63755_Pper_Dia2013_COI	KWIB25	1	BC63755_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	CTTAGTGGCATTAATTCGDAATCC	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.975	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63755_Sspa_Oph2007_COI	KWIB25	1	BC63755_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	GAAGGCCTTCCGATGATC	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63755_Upin_Bot2011_COI	KWIB25	1	BC63755_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	CTTTAATTACAGCGTTTTTATTGTTGT	115.75317
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63756_Cjap_Sim2018_COI	KWIB20	1	BC63756_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63756_Mgig_Bot2012_COI	KWIB20	1	BC63756_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	TCTTATTCGTTGGAGACTTTATAACCCT	115.75447
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63757_Cmae_Bot2011_COI	KWIB15	1	BC63757_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	ATGAACAGTCTATCCTCCTTTAG	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63757_Msal_Bot2012_COI	KWIB15	1	BC63757_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	GYTAGTTCCRATGATGTTAGCTG	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63757_Pvir_Dia2013_COI	KWIB15	1	BC63757_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	CTTAGTGGCATTAATTCGDAATCC	115.75598
BC63757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24372	WGS84	BC63757	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63757_Rhar_Sim2018_COI	KWIB15	1	BC63757_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63757	Sample	BC63757	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB15	COI	45	BC63757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB15	CTGCTGCTATTGCTCATG	115.75598
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63759_Aamu_Bax2006_COI	KWIB5	1	BC63759_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	GCACAACCGGGATCTTTACTTC	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63759_Pcan_Bot2011_IGS	KWIB5	1	BC63759_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB5	IGS	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	CGTAATCCTCAGTACTGGCTA	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63759_Pper_Dia2013_COI	KWIB5	1	BC63759_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	CTTAGTGGCATTAATTCGDAATCC	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.076	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63759_Sspa_Oph2007_COI	KWIB5	1	BC63759_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	GAAGGCCTTCCGATGATC	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63759_Upin_Bot2011_COI	KWIB5	1	BC63759_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	CTTTAATTACAGCGTTTTTATTGTTGT	115.74466
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63760_Cmae_Bot2011_COI	KWIB59	1	BC63760_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	ATGAACAGTCTATCCTCCTTTAG	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63760_Msal_Bot2012_COI	KWIB59	1	BC63760_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	GYTAGTTCCRATGATGTTAGCTG	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63760_Pvir_Dia2013_COI	KWIB59	1	BC63760_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	CTTAGTGGCATTAATTCGDAATCC	115.77164
BC63760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18792	WGS84	BC63760	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63760_Rhar_Sim2018_COI	KWIB59	1	BC63760_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63760	Sample	BC63760	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB59	COI	45	BC63760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB59	CTGCTGCTATTGCTCATG	115.77164
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63762_Aamu_Bax2006_COI	KWIB57	1	BC63762_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	GCACAACCGGGATCTTTACTTC	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63762_Pcan_Bot2011_IGS	KWIB57	1	BC63762_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB57	IGS	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	CGTAATCCTCAGTACTGGCTA	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63762_Pper_Dia2013_COI	KWIB57	1	BC63762_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	CTTAGTGGCATTAATTCGDAATCC	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63762_Sspa_Oph2007_COI	KWIB57	1	BC63762_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	GAAGGCCTTCCGATGATC	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63762_Upin_Bot2011_COI	KWIB57	1	BC63762_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	CTTTAATTACAGCGTTTTTATTGTTGT	115.7729
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63763_Cjap_Sim2018_COI	KWIB56	1	BC63763_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63763_Mgig_Bot2012_COI	KWIB56	1	BC63763_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	TCTTATTCGTTGGAGACTTTATAACCCT	115.7711
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63764_Cmae_Bot2011_COI	KWIB54	1	BC63764_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	ATGAACAGTCTATCCTCCTTTAG	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63764_Msal_Bot2012_COI	KWIB54	1	BC63764_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	GYTAGTTCCRATGATGTTAGCTG	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63764_Pvir_Dia2013_COI	KWIB54	1	BC63764_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	CTTAGTGGCATTAATTCGDAATCC	115.77258
BC63764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19213	WGS84	BC63764	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63764_Rhar_Sim2018_COI	KWIB54	1	BC63764_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63764	Sample	BC63764	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB54	COI	45	BC63764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB54	CTGCTGCTATTGCTCATG	115.77258
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63766_Aamu_Bax2006_COI	KWIB52	1	BC63766_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	GCACAACCGGGATCTTTACTTC	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63766_Pcan_Bot2011_IGS	KWIB52	1	BC63766_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB52	IGS	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	CGTAATCCTCAGTACTGGCTA	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63766_Pper_Dia2013_COI	KWIB52	1	BC63766_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	CTTAGTGGCATTAATTCGDAATCC	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63766_Sspa_Oph2007_COI	KWIB52	1	BC63766_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	GAAGGCCTTCCGATGATC	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63766_Upin_Bot2011_COI	KWIB52	1	BC63766_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	CTTTAATTACAGCGTTTTTATTGTTGT	115.76958
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63767_Cjap_Sim2018_COI	KWIB51	1	BC63767_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63767_Mgig_Bot2012_COI	KWIB51	1	BC63767_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	TCTTATTCGTTGGAGACTTTATAACCCT	115.76639
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63768_Cmae_Bot2011_COI	KWIB49	1	BC63768_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	ATGAACAGTCTATCCTCCTTTAG	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63768_Msal_Bot2012_COI	KWIB49	1	BC63768_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	GYTAGTTCCRATGATGTTAGCTG	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63768_Pvir_Dia2013_COI	KWIB49	1	BC63768_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	CTTAGTGGCATTAATTCGDAATCC	115.76462
BC63768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20922	WGS84	BC63768	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63768_Rhar_Sim2018_COI	KWIB49	1	BC63768_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63768	Sample	BC63768	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB49	COI	45	BC63768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB49	CTGCTGCTATTGCTCATG	115.76462
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21047	WGS84	BC63770	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.645	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63770_Asen_Wil2025_COI	KWIB47	1	BC63770_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Present	Sample	Asen_Wil2025_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	GTTGTTGTTACAACACATGCTCTTTTG	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63770_Cjap_Sim2018_COI	KWIB47	1	BC63770_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63770_Mgig_Bot2012_COI	KWIB47	1	BC63770_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	TCTTATTCGTTGGAGACTTTATAACCCT	115.76457
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63771_Cmae_Bot2011_COI	KWIB46	1	BC63771_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	ATGAACAGTCTATCCTCCTTTAG	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63771_Msal_Bot2012_COI	KWIB46	1	BC63771_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	GYTAGTTCCRATGATGTTAGCTG	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63771_Pvir_Dia2013_COI	KWIB46	1	BC63771_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	CTTAGTGGCATTAATTCGDAATCC	115.76322
BC63771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21083	WGS84	BC63771	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63771_Rhar_Sim2018_COI	KWIB46	1	BC63771_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63771	Sample	BC63771	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB46	COI	45	BC63771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB46	CTGCTGCTATTGCTCATG	115.76322
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63773_Aamu_Bax2006_COI	KWIB43	1	BC63773_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	GCACAACCGGGATCTTTACTTC	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63773_Pcan_Bot2011_IGS	KWIB43	1	BC63773_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB43	IGS	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	CGTAATCCTCAGTACTGGCTA	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63773_Pper_Dia2013_COI	KWIB43	1	BC63773_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	CTTAGTGGCATTAATTCGDAATCC	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63773_Sspa_Oph2007_COI	KWIB43	1	BC63773_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	GAAGGCCTTCCGATGATC	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63773_Upin_Bot2011_COI	KWIB43	1	BC63773_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	CTTTAATTACAGCGTTTTTATTGTTGT	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21242	WGS84	BC63773	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63773_Vgib_Wil2025_COI	KWIB43	1	BC63773_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	CCCCTCTTTACAAGTTCTTTAGGGT	115.75911
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22943	WGS84	BC63774	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63774_Asen_Wil2025_COI	KWIB42	1	BC63774_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	GTTGTTGTTACAACACATGCTCTTTTG	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63774_Cjap_Sim2018_COI	KWIB42	1	BC63774_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63774_Mgig_Bot2012_COI	KWIB42	1	BC63774_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	TCTTATTCGTTGGAGACTTTATAACCCT	115.75677
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63775_Cmae_Bot2011_COI	KWIB41	1	BC63775_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	ATGAACAGTCTATCCTCCTTTAG	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63775_Msal_Bot2012_COI	KWIB41	1	BC63775_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	GYTAGTTCCRATGATGTTAGCTG	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63775_Pvir_Dia2013_COI	KWIB41	1	BC63775_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	CTTAGTGGCATTAATTCGDAATCC	115.75533
BC63775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22831	WGS84	BC63775	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63775_Rhar_Sim2018_COI	KWIB41	1	BC63775_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63775	Sample	BC63775	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB41	COI	45	BC63775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB41	CTGCTGCTATTGCTCATG	115.75533
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63777_Aamu_Bax2006_COI	KWIB38	1	BC63777_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	GCACAACCGGGATCTTTACTTC	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63777_Pcan_Bot2011_IGS	KWIB38	1	BC63777_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB38	IGS	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	CGTAATCCTCAGTACTGGCTA	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63777_Pper_Dia2013_COI	KWIB38	1	BC63777_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	CTTAGTGGCATTAATTCGDAATCC	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63777_Sspa_Oph2007_COI	KWIB38	1	BC63777_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	GAAGGCCTTCCGATGATC	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63777_Upin_Bot2011_COI	KWIB38	1	BC63777_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	CTTTAATTACAGCGTTTTTATTGTTGT	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22969	WGS84	BC63777	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63777_Vgib_Wil2025_COI	KWIB38	1	BC63777_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	CCCCTCTTTACAAGTTCTTTAGGGT	115.75292
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23223	WGS84	BC63778	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63778_Asen_Wil2025_COI	KWIB37	1	BC63778_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	GTTGTTGTTACAACACATGCTCTTTTG	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63778_Cjap_Sim2018_COI	KWIB37	1	BC63778_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63778_Mgig_Bot2012_COI	KWIB37	1	BC63778_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	TCTTATTCGTTGGAGACTTTATAACCCT	115.7565
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63779_Cmae_Bot2011_COI	KWIB36	1	BC63779_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	ATGAACAGTCTATCCTCCTTTAG	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63779_Msal_Bot2012_COI	KWIB36	1	BC63779_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	GYTAGTTCCRATGATGTTAGCTG	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63779_Pvir_Dia2013_COI	KWIB36	1	BC63779_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	CTTAGTGGCATTAATTCGDAATCC	115.75378
BC63779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23195	WGS84	BC63779	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63779_Rhar_Sim2018_COI	KWIB36	1	BC63779_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63779	Sample	BC63779	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB36	COI	45	BC63779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB36	CTGCTGCTATTGCTCATG	115.75378
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63780_Aamu_Bax2006_COI	KWIB34	1	BC63780_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	GCACAACCGGGATCTTTACTTC	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63780_Pcan_Bot2011_IGS	KWIB34	1	BC63780_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB34	IGS	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	CGTAATCCTCAGTACTGGCTA	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63780_Pper_Dia2013_COI	KWIB34	1	BC63780_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	CTTAGTGGCATTAATTCGDAATCC	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63780_Sspa_Oph2007_COI	KWIB34	1	BC63780_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	GAAGGCCTTCCGATGATC	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63780_Upin_Bot2011_COI	KWIB34	1	BC63780_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	CTTTAATTACAGCGTTTTTATTGTTGT	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23233	WGS84	BC63780	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63780_Vgib_Wil2025_COI	KWIB34	1	BC63780_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	CCCCTCTTTACAAGTTCTTTAGGGT	115.75612
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23243	WGS84	BC63781	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.078	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63781_Asen_Wil2025_COI	KWIB33	1	BC63781_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Present	Sample	Asen_Wil2025_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	GTTGTTGTTACAACACATGCTCTTTTG	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63781_Cjap_Sim2018_COI	KWIB33	1	BC63781_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63781_Mgig_Bot2012_COI	KWIB33	1	BC63781_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	TCTTATTCGTTGGAGACTTTATAACCCT	115.75438
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63782_Cmae_Bot2011_COI	KWIB32	1	BC63782_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	ATGAACAGTCTATCCTCCTTTAG	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63782_Msal_Bot2012_COI	KWIB32	1	BC63782_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	GYTAGTTCCRATGATGTTAGCTG	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63782_Pvir_Dia2013_COI	KWIB32	1	BC63782_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	CTTAGTGGCATTAATTCGDAATCC	115.75315
BC63782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23397	WGS84	BC63782	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63782_Rhar_Sim2018_COI	KWIB32	1	BC63782_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63782	Sample	BC63782	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB32	COI	45	BC63782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB32	CTGCTGCTATTGCTCATG	115.75315
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63784_Aamu_Bax2006_COI	KWIB29	1	BC63784_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	GCACAACCGGGATCTTTACTTC	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63784_Pcan_Bot2011_IGS	KWIB29	1	BC63784_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB29	IGS	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	CGTAATCCTCAGTACTGGCTA	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63784_Pper_Dia2013_COI	KWIB29	1	BC63784_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	CTTAGTGGCATTAATTCGDAATCC	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63784_Sspa_Oph2007_COI	KWIB29	1	BC63784_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	GAAGGCCTTCCGATGATC	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63784_Upin_Bot2011_COI	KWIB29	1	BC63784_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	CTTTAATTACAGCGTTTTTATTGTTGT	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23605	WGS84	BC63784	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63784_Vgib_Wil2025_COI	KWIB29	1	BC63784_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	CCCCTCTTTACAAGTTCTTTAGGGT	115.75441
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.2377	WGS84	BC63785	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63785_Asen_Wil2025_COI	KWIB28	1	BC63785_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	GTTGTTGTTACAACACATGCTCTTTTG	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63785_Cjap_Sim2018_COI	KWIB28	1	BC63785_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63785_Mgig_Bot2012_COI	KWIB28	1	BC63785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	TCTTATTCGTTGGAGACTTTATAACCCT	115.75654
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63786_Cmae_Bot2011_COI	KWIB27	1	BC63786_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	ATGAACAGTCTATCCTCCTTTAG	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63786_Msal_Bot2012_COI	KWIB27	1	BC63786_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	GYTAGTTCCRATGATGTTAGCTG	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63786_Pvir_Dia2013_COI	KWIB27	1	BC63786_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	CTTAGTGGCATTAATTCGDAATCC	115.75851
BC63786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23741	WGS84	BC63786	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63786_Rhar_Sim2018_COI	KWIB27	1	BC63786_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63786	Sample	BC63786	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB27	COI	45	BC63786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB27	CTGCTGCTATTGCTCATG	115.75851
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63788_Aamu_Bax2006_COI	KWIB24	1	BC63788_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	GCACAACCGGGATCTTTACTTC	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63788_Pcan_Bot2011_IGS	KWIB24	1	BC63788_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB24	IGS	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	CGTAATCCTCAGTACTGGCTA	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63788_Pper_Dia2013_COI	KWIB24	1	BC63788_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	CTTAGTGGCATTAATTCGDAATCC	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.26	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63788_Sspa_Oph2007_COI	KWIB24	1	BC63788_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	GAAGGCCTTCCGATGATC	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63788_Upin_Bot2011_COI	KWIB24	1	BC63788_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	CTTTAATTACAGCGTTTTTATTGTTGT	115.75724
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63789_Cjap_Sim2018_COI	KWIB23	1	BC63789_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63789_Mgig_Bot2012_COI	KWIB23	1	BC63789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	TCTTATTCGTTGGAGACTTTATAACCCT	115.75448
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63791_Aamu_Bax2006_COI	KWIB21	1	BC63791_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	GCACAACCGGGATCTTTACTTC	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63791_Pcan_Bot2011_IGS	KWIB21	1	BC63791_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB21	IGS	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	CGTAATCCTCAGTACTGGCTA	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63791_Pper_Dia2013_COI	KWIB21	1	BC63791_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	CTTAGTGGCATTAATTCGDAATCC	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.02	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63791_Sspa_Oph2007_COI	KWIB21	1	BC63791_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	GAAGGCCTTCCGATGATC	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63791_Upin_Bot2011_COI	KWIB21	1	BC63791_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	CTTTAATTACAGCGTTTTTATTGTTGT	115.75679
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63792_Cjap_Sim2018_COI	KWIB19	1	BC63792_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63792_Mgig_Bot2012_COI	KWIB19	1	BC63792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	TCTTATTCGTTGGAGACTTTATAACCCT	115.75341
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63793_Cmae_Bot2011_COI	KWIB18	1	BC63793_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	ATGAACAGTCTATCCTCCTTTAG	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63793_Msal_Bot2012_COI	KWIB18	1	BC63793_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	GYTAGTTCCRATGATGTTAGCTG	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63793_Pvir_Dia2013_COI	KWIB18	1	BC63793_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	CTTAGTGGCATTAATTCGDAATCC	115.75699
BC63793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24243	WGS84	BC63793	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63793_Rhar_Sim2018_COI	KWIB18	1	BC63793_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63793	Sample	BC63793	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB18	COI	45	BC63793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB18	CTGCTGCTATTGCTCATG	115.75699
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63795_Aamu_Bax2006_COI	KWIB16	1	BC63795_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	GCACAACCGGGATCTTTACTTC	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63795_Pcan_Bot2011_IGS	KWIB16	1	BC63795_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB16	IGS	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	CGTAATCCTCAGTACTGGCTA	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63795_Pper_Dia2013_COI	KWIB16	1	BC63795_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	CTTAGTGGCATTAATTCGDAATCC	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63795_Sspa_Oph2007_COI	KWIB16	1	BC63795_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	GAAGGCCTTCCGATGATC	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63795_Upin_Bot2011_COI	KWIB16	1	BC63795_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	CTTTAATTACAGCGTTTTTATTGTTGT	115.75743
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63796_Cjap_Sim2018_COI	KWIB14	1	BC63796_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63796_Mgig_Bot2012_COI	KWIB14	1	BC63796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	TCTTATTCGTTGGAGACTTTATAACCCT	115.7535
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63797_Cmae_Bot2011_COI	KWIB13	1	BC63797_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	ATGAACAGTCTATCCTCCTTTAG	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63797_Msal_Bot2012_COI	KWIB13	1	BC63797_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	GYTAGTTCCRATGATGTTAGCTG	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63797_Pvir_Dia2013_COI	KWIB13	1	BC63797_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	CTTAGTGGCATTAATTCGDAATCC	115.75209
BC63797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24225	WGS84	BC63797	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63797_Rhar_Sim2018_COI	KWIB13	1	BC63797_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63797	Sample	BC63797	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB13	COI	45	BC63797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB13	CTGCTGCTATTGCTCATG	115.75209
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63799_Aamu_Bax2006_COI	KWIB11	1	BC63799_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	GCACAACCGGGATCTTTACTTC	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63799_Pcan_Bot2011_IGS	KWIB11	1	BC63799_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB11	IGS	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	CGTAATCCTCAGTACTGGCTA	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63799_Pper_Dia2013_COI	KWIB11	1	BC63799_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	CTTAGTGGCATTAATTCGDAATCC	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63799_Sspa_Oph2007_COI	KWIB11	1	BC63799_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	GAAGGCCTTCCGATGATC	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63799_Upin_Bot2011_COI	KWIB11	1	BC63799_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	CTTTAATTACAGCGTTTTTATTGTTGT	115.74522
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63800_Cjap_Sim2018_COI	KWIB9	1	BC63800_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63800_Mgig_Bot2012_COI	KWIB9	1	BC63800_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	TCTTATTCGTTGGAGACTTTATAACCCT	115.74274
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63801_Cmae_Bot2011_COI	KWIB8	1	BC63801_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	ATGAACAGTCTATCCTCCTTTAG	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63801_Msal_Bot2012_COI	KWIB8	1	BC63801_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	GYTAGTTCCRATGATGTTAGCTG	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63801_Pvir_Dia2013_COI	KWIB8	1	BC63801_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	CTTAGTGGCATTAATTCGDAATCC	115.74448
BC63801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25318	WGS84	BC63801	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63801_Rhar_Sim2018_COI	KWIB8	1	BC63801_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63801	Sample	BC63801	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB8	COI	45	BC63801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB8	CTGCTGCTATTGCTCATG	115.74448
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63803_Aamu_Bax2006_COI	KWIB6	1	BC63803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	GCACAACCGGGATCTTTACTTC	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63803_Pcan_Bot2011_IGS	KWIB6	1	BC63803_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB6	IGS	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	CGTAATCCTCAGTACTGGCTA	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63803_Pper_Dia2013_COI	KWIB6	1	BC63803_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	CTTAGTGGCATTAATTCGDAATCC	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63803_Sspa_Oph2007_COI	KWIB6	1	BC63803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	GAAGGCCTTCCGATGATC	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63803_Upin_Bot2011_COI	KWIB6	1	BC63803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	CTTTAATTACAGCGTTTTTATTGTTGT	115.74078
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63804_Cjap_Sim2018_COI	KWIB4	1	BC63804_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63804_Mgig_Bot2012_COI	KWIB4	1	BC63804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	TCTTATTCGTTGGAGACTTTATAACCCT	115.74288
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63805_Cmae_Bot2011_COI	KWIB3	1	BC63805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	ATGAACAGTCTATCCTCCTTTAG	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63805_Msal_Bot2012_COI	KWIB3	1	BC63805_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	GYTAGTTCCRATGATGTTAGCTG	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63805_Pvir_Dia2013_COI	KWIB3	1	BC63805_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	CTTAGTGGCATTAATTCGDAATCC	115.74123
BC63805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.255	WGS84	BC63805	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63805_Rhar_Sim2018_COI	KWIB3	1	BC63805_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63805	Sample	BC63805	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB3	COI	45	BC63805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB3	CTGCTGCTATTGCTCATG	115.74123
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63807_Aamu_Bax2006_COI	KWIB1	1	BC63807_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	GCACAACCGGGATCTTTACTTC	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63807_Pcan_Bot2011_IGS	KWIB1	1	BC63807_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB1	IGS	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	CGTAATCCTCAGTACTGGCTA	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63807_Pper_Dia2013_COI	KWIB1	1	BC63807_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	CTTAGTGGCATTAATTCGDAATCC	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.851	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63807_Sspa_Oph2007_COI	KWIB1	1	BC63807_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	GAAGGCCTTCCGATGATC	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63807_Upin_Bot2011_COI	KWIB1	1	BC63807_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	CTTTAATTACAGCGTTTTTATTGTTGT	115.74057
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63808_Cjap_Sim2018_COI	BUNB20	1	BC63808_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63808_Mgig_Bot2012_COI	BUNB20	1	BC63808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	TCTTATTCGTTGGAGACTTTATAACCCT	115.64503
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63809_Cmae_Bot2011_COI	BUNB15	1	BC63809_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	ATGAACAGTCTATCCTCCTTTAG	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63809_Msal_Bot2012_COI	BUNB15	1	BC63809_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	GYTAGTTCCRATGATGTTAGCTG	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63809_Pvir_Dia2013_COI	BUNB15	1	BC63809_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	CTTAGTGGCATTAATTCGDAATCC	115.66455
BC63809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32211	WGS84	BC63809	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63809_Rhar_Sim2018_COI	BUNB15	1	BC63809_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63809	Sample	BC63809	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB15	COI	45	BC63809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB15	CTGCTGCTATTGCTCATG	115.66455
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63810_Aamu_Bax2006_COI	BUNB10	1	BC63810_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	GCACAACCGGGATCTTTACTTC	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63810_Pcan_Bot2011_IGS	BUNB10	1	BC63810_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB10	IGS	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	CGTAATCCTCAGTACTGGCTA	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63810_Pper_Dia2013_COI	BUNB10	1	BC63810_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	CTTAGTGGCATTAATTCGDAATCC	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63810_Sspa_Oph2007_COI	BUNB10	1	BC63810_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	GAAGGCCTTCCGATGATC	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63810_Upin_Bot2011_COI	BUNB10	1	BC63810_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	CTTTAATTACAGCGTTTTTATTGTTGT	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.32313	WGS84	BC63810	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63810_Vgib_Wil2025_COI	BUNB10	1	BC63810_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	CCCCTCTTTACAAGTTCTTTAGGGT	115.66527
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.32473	WGS84	BC63811	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63811_Asen_Wil2025_COI	BUNB5	1	BC63811_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	GTTGTTGTTACAACACATGCTCTTTTG	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63811_Cjap_Sim2018_COI	BUNB5	1	BC63811_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63811_Mgig_Bot2012_COI	BUNB5	1	BC63811_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	TCTTATTCGTTGGAGACTTTATAACCCT	115.66712
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63812_Cmae_Bot2011_COI	BUNB23	1	BC63812_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	ATGAACAGTCTATCCTCCTTTAG	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63812_Msal_Bot2012_COI	BUNB23	1	BC63812_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	GYTAGTTCCRATGATGTTAGCTG	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63812_Pvir_Dia2013_COI	BUNB23	1	BC63812_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	CTTAGTGGCATTAATTCGDAATCC	115.64711
BC63812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30654	WGS84	BC63812	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63812_Rhar_Sim2018_COI	BUNB23	1	BC63812_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63812	Sample	BC63812	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB23	COI	45	BC63812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB23	CTGCTGCTATTGCTCATG	115.64711
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63814_Aamu_Bax2006_COI	BUNB21	1	BC63814_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	GCACAACCGGGATCTTTACTTC	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63814_Pcan_Bot2011_IGS	BUNB21	1	BC63814_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB21	IGS	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	CGTAATCCTCAGTACTGGCTA	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63814_Pper_Dia2013_COI	BUNB21	1	BC63814_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	CTTAGTGGCATTAATTCGDAATCC	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63814_Sspa_Oph2007_COI	BUNB21	1	BC63814_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	GAAGGCCTTCCGATGATC	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63814_Upin_Bot2011_COI	BUNB21	1	BC63814_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	CTTTAATTACAGCGTTTTTATTGTTGT	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.30753	WGS84	BC63814	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63814_Vgib_Wil2025_COI	BUNB21	1	BC63814_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	CCCCTCTTTACAAGTTCTTTAGGGT	115.64716
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63815_Cjap_Sim2018_COI	BUNB19	1	BC63815_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63815_Mgig_Bot2012_COI	BUNB19	1	BC63815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	TCTTATTCGTTGGAGACTTTATAACCCT	115.64399
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63816_Cmae_Bot2011_COI	BUNB18	1	BC63816_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	ATGAACAGTCTATCCTCCTTTAG	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63816_Msal_Bot2012_COI	BUNB18	1	BC63816_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	GYTAGTTCCRATGATGTTAGCTG	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63816_Pvir_Dia2013_COI	BUNB18	1	BC63816_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	CTTAGTGGCATTAATTCGDAATCC	115.64277
BC63816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30947	WGS84	BC63816	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63816_Rhar_Sim2018_COI	BUNB18	1	BC63816_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63816	Sample	BC63816	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB18	COI	45	BC63816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB18	CTGCTGCTATTGCTCATG	115.64277
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63818_Aamu_Bax2006_COI	BUNB16	1	BC63818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	GCACAACCGGGATCTTTACTTC	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63818_Pcan_Bot2011_IGS	BUNB16	1	BC63818_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB16	IGS	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	CGTAATCCTCAGTACTGGCTA	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63818_Pper_Dia2013_COI	BUNB16	1	BC63818_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	CTTAGTGGCATTAATTCGDAATCC	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63818_Sspa_Oph2007_COI	BUNB16	1	BC63818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	GAAGGCCTTCCGATGATC	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63818_Upin_Bot2011_COI	BUNB16	1	BC63818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	CTTTAATTACAGCGTTTTTATTGTTGT	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63818_Vgib_Wil2025_COI	BUNB16	1	BC63818_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	CCCCTCTTTACAAGTTCTTTAGGGT	115.6614
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63819_Asen_Wil2025_COI	BUNB14	1	BC63819_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	GTTGTTGTTACAACACATGCTCTTTTG	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63819_Cjap_Sim2018_COI	BUNB14	1	BC63819_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63819_Mgig_Bot2012_COI	BUNB14	1	BC63819_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	TCTTATTCGTTGGAGACTTTATAACCCT	115.6627
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63821_Aamu_Bax2006_COI	BUNB12	1	BC63821_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	GCACAACCGGGATCTTTACTTC	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63821_Pcan_Bot2011_IGS	BUNB12	1	BC63821_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB12	IGS	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	CGTAATCCTCAGTACTGGCTA	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63821_Pper_Dia2013_COI	BUNB12	1	BC63821_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	CTTAGTGGCATTAATTCGDAATCC	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63821_Sspa_Oph2007_COI	BUNB12	1	BC63821_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	GAAGGCCTTCCGATGATC	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63821_Upin_Bot2011_COI	BUNB12	1	BC63821_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	CTTTAATTACAGCGTTTTTATTGTTGT	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63821_Vgib_Wil2025_COI	BUNB12	1	BC63821_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	CCCCTCTTTACAAGTTCTTTAGGGT	115.66029
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63822_Asen_Wil2025_COI	BUNB11	1	BC63822_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	GTTGTTGTTACAACACATGCTCTTTTG	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63822_Cjap_Sim2018_COI	BUNB11	1	BC63822_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63822_Mgig_Bot2012_COI	BUNB11	1	BC63822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	TCTTATTCGTTGGAGACTTTATAACCCT	115.6586
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63823_Cmae_Bot2011_COI	BUNB9	1	BC63823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	ATGAACAGTCTATCCTCCTTTAG	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63823_Msal_Bot2012_COI	BUNB9	1	BC63823_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	GYTAGTTCCRATGATGTTAGCTG	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63823_Pvir_Dia2013_COI	BUNB9	1	BC63823_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	CTTAGTGGCATTAATTCGDAATCC	115.66373
BC63823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3226	WGS84	BC63823	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63823_Rhar_Sim2018_COI	BUNB9	1	BC63823_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63823	Sample	BC63823	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB9	COI	45	BC63823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB9	CTGCTGCTATTGCTCATG	115.66373
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63825_Aamu_Bax2006_COI	BUNB7	1	BC63825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	GCACAACCGGGATCTTTACTTC	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63825_Pcan_Bot2011_IGS	BUNB7	1	BC63825_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB7	IGS	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	CGTAATCCTCAGTACTGGCTA	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63825_Pper_Dia2013_COI	BUNB7	1	BC63825_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	CTTAGTGGCATTAATTCGDAATCC	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63825_Sspa_Oph2007_COI	BUNB7	1	BC63825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	GAAGGCCTTCCGATGATC	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63825_Upin_Bot2011_COI	BUNB7	1	BC63825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	CTTTAATTACAGCGTTTTTATTGTTGT	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63825_Vgib_Wil2025_COI	BUNB7	1	BC63825_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	CCCCTCTTTACAAGTTCTTTAGGGT	115.66108
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63826_Asen_Wil2025_COI	BUNB6	1	BC63826_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	GTTGTTGTTACAACACATGCTCTTTTG	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63826_Cjap_Sim2018_COI	BUNB6	1	BC63826_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63826_Mgig_Bot2012_COI	BUNB6	1	BC63826_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	TCTTATTCGTTGGAGACTTTATAACCCT	115.66757
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63827_Cmae_Bot2011_COI	BUNB4	1	BC63827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	ATGAACAGTCTATCCTCCTTTAG	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63827_Msal_Bot2012_COI	BUNB4	1	BC63827_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	GYTAGTTCCRATGATGTTAGCTG	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63827_Pvir_Dia2013_COI	BUNB4	1	BC63827_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	CTTAGTGGCATTAATTCGDAATCC	115.66528
BC63827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3244	WGS84	BC63827	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63827_Rhar_Sim2018_COI	BUNB4	1	BC63827_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63827	Sample	BC63827	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB4	COI	45	BC63827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB4	CTGCTGCTATTGCTCATG	115.66528
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63829_Aamu_Bax2006_COI	BUNB2	1	BC63829_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	GCACAACCGGGATCTTTACTTC	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63829_Pcan_Bot2011_IGS	BUNB2	1	BC63829_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB2	IGS	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	CGTAATCCTCAGTACTGGCTA	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63829_Pper_Dia2013_COI	BUNB2	1	BC63829_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	CTTAGTGGCATTAATTCGDAATCC	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63829_Sspa_Oph2007_COI	BUNB2	1	BC63829_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	GAAGGCCTTCCGATGATC	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63829_Upin_Bot2011_COI	BUNB2	1	BC63829_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	CTTTAATTACAGCGTTTTTATTGTTGT	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63829_Vgib_Wil2025_COI	BUNB2	1	BC63829_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	CCCCTCTTTACAAGTTCTTTAGGGT	115.66723
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63830_Cmae_Bot2011_COI	BUNB1	1	BC63830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	ATGAACAGTCTATCCTCCTTTAG	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63830_Msal_Bot2012_COI	BUNB1	1	BC63830_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	GYTAGTTCCRATGATGTTAGCTG	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63830_Pvir_Dia2013_COI	BUNB1	1	BC63830_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	CTTAGTGGCATTAATTCGDAATCC	115.66652
BC63830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32695	WGS84	BC63830	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63830_Rhar_Sim2018_COI	BUNB1	1	BC63830_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63830	Sample	BC63830	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB1	COI	45	BC63830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB1	CTGCTGCTATTGCTCATG	115.66652
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63832_Aamu_Bax2006_COI	GERB10	1	BC63832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	GCACAACCGGGATCTTTACTTC	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63832_Pcan_Bot2011_IGS	GERB10	1	BC63832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB10	IGS	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	CGTAATCCTCAGTACTGGCTA	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63832_Pper_Dia2013_COI	GERB10	1	BC63832_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Pper_Dia2013_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	CTTAGTGGCATTAATTCGDAATCC	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63832_Sspa_Oph2007_COI	GERB10	1	BC63832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	GAAGGCCTTCCGATGATC	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63832_Upin_Bot2011_COI	GERB10	1	BC63832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Upin_Bot2011_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	CTTTAATTACAGCGTTTTTATTGTTGT	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77465	WGS84	BC63832	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63832_Vgib_Wil2025_COI	GERB10	1	BC63832_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	CCCCTCTTTACAAGTTCTTTAGGGT	114.60076
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77534	WGS84	BC63833	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63833_Asen_Wil2025_COI	GERB5	1	BC63833_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Asen_Wil2025_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	GTTGTTGTTACAACACATGCTCTTTTG	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63833_Cjap_Sim2018_COI	GERB5	1	BC63833_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63833_Mgig_Bot2012_COI	GERB5	1	BC63833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	TCTTATTCGTTGGAGACTTTATAACCCT	114.59844
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63834_Cmae_Bot2011_COI	GERB14	1	BC63834_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	ATGAACAGTCTATCCTCCTTTAG	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63834_Msal_Bot2012_COI	GERB14	1	BC63834_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Msal_Bot2012_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	GYTAGTTCCRATGATGTTAGCTG	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63834_Pvir_Dia2013_COI	GERB14	1	BC63834_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	CTTAGTGGCATTAATTCGDAATCC	114.60041
BC63834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7731	WGS84	BC63834	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63834_Rhar_Sim2018_COI	GERB14	1	BC63834_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63834	Sample	BC63834	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB14	COI	45	BC63834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB14	CTGCTGCTATTGCTCATG	114.60041
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63836_Aamu_Bax2006_COI	GERB12	1	BC63836_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	GCACAACCGGGATCTTTACTTC	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63836_Pcan_Bot2011_IGS	GERB12	1	BC63836_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB12	IGS	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	CGTAATCCTCAGTACTGGCTA	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63836_Pper_Dia2013_COI	GERB12	1	BC63836_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Pper_Dia2013_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	CTTAGTGGCATTAATTCGDAATCC	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63836_Sspa_Oph2007_COI	GERB12	1	BC63836_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	GAAGGCCTTCCGATGATC	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63836_Upin_Bot2011_COI	GERB12	1	BC63836_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Upin_Bot2011_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	CTTTAATTACAGCGTTTTTATTGTTGT	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77283	WGS84	BC63836	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63836_Vgib_Wil2025_COI	GERB12	1	BC63836_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	CCCCTCTTTACAAGTTCTTTAGGGT	114.59728
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77278	WGS84	BC63837	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63837_Asen_Wil2025_COI	GERB11	1	BC63837_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Asen_Wil2025_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	GTTGTTGTTACAACACATGCTCTTTTG	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63837_Cjap_Sim2018_COI	GERB11	1	BC63837_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59585
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63838_Cmae_Bot2011_COI	GERB9	1	BC63838_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	ATGAACAGTCTATCCTCCTTTAG	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63838_Msal_Bot2012_COI	GERB9	1	BC63838_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Msal_Bot2012_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	GYTAGTTCCRATGATGTTAGCTG	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63838_Pvir_Dia2013_COI	GERB9	1	BC63838_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	CTTAGTGGCATTAATTCGDAATCC	114.59939
BC63838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.7742	WGS84	BC63838	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63838_Rhar_Sim2018_COI	GERB9	1	BC63838_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63838	Sample	BC63838	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB9	COI	45	BC63838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB9	CTGCTGCTATTGCTCATG	114.59939
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63840_Cjap_Sim2018_COI	GERB7	1	BC63840_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59635
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63841_Cmae_Bot2011_COI	GERB6	1	BC63841_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	ATGAACAGTCTATCCTCCTTTAG	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63841_Msal_Bot2012_COI	GERB6	1	BC63841_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Msal_Bot2012_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	GYTAGTTCCRATGATGTTAGCTG	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63841_Pvir_Dia2013_COI	GERB6	1	BC63841_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	CTTAGTGGCATTAATTCGDAATCC	114.59995
BC63841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77542	WGS84	BC63841	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63841_Rhar_Sim2018_COI	GERB6	1	BC63841_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63841	Sample	BC63841	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB6	COI	45	BC63841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB6	CTGCTGCTATTGCTCATG	114.59995
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63843_Aamu_Bax2006_COI	GERB3	1	BC63843_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	GCACAACCGGGATCTTTACTTC	114.59538
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63843_Pcan_Bot2011_IGS	GERB3	1	BC63843_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB3	IGS	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	CGTAATCCTCAGTACTGGCTA	114.59538
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63843_Pper_Dia2013_COI	GERB3	1	BC63843_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Pper_Dia2013_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	CTTAGTGGCATTAATTCGDAATCC	114.59538
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63843_Sspa_Oph2007_COI	GERB3	1	BC63843_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	GAAGGCCTTCCGATGATC	114.59538
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63843_Upin_Bot2011_COI	GERB3	1	BC63843_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Upin_Bot2011_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	CTTTAATTACAGCGTTTTTATTGTTGT	114.59538
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63844_Cjap_Sim2018_COI	GERB2	1	BC63844_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63844_Mgig_Bot2012_COI	GERB2	1	BC63844_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Mgig_Bot2012_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	TCTTATTCGTTGGAGACTTTATAACCCT	114.59591
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63845_Cmae_Bot2011_COI	KWIA60	1	BC63845_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	ATGAACAGTCTATCCTCCTTTAG	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63845_Msal_Bot2012_COI	KWIA60	1	BC63845_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	GYTAGTTCCRATGATGTTAGCTG	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63845_Pvir_Dia2013_COI	KWIA60	1	BC63845_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	CTTAGTGGCATTAATTCGDAATCC	115.7731
BC63845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18843	WGS84	BC63845	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63845_Rhar_Sim2018_COI	KWIA60	1	BC63845_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63845	Sample	BC63845	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA60	COI	45	BC63845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA60	CTGCTGCTATTGCTCATG	115.7731
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63847_Aamu_Bax2006_COI	KWIA58	1	BC63847_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	GCACAACCGGGATCTTTACTTC	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63847_Pcan_Bot2011_IGS	KWIA58	1	BC63847_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA58	IGS	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	CGTAATCCTCAGTACTGGCTA	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63847_Pper_Dia2013_COI	KWIA58	1	BC63847_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	CTTAGTGGCATTAATTCGDAATCC	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63847_Sspa_Oph2007_COI	KWIA58	1	BC63847_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	GAAGGCCTTCCGATGATC	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63847_Upin_Bot2011_COI	KWIA58	1	BC63847_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	CTTTAATTACAGCGTTTTTATTGTTGT	115.76965
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63848_Cjap_Sim2018_COI	KWIA57	1	BC63848_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63848_Mgig_Bot2012_COI	KWIA57	1	BC63848_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	TCTTATTCGTTGGAGACTTTATAACCCT	115.77329
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63849_Cmae_Bot2011_COI	KWIA56	1	BC63849_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	ATGAACAGTCTATCCTCCTTTAG	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63849_Msal_Bot2012_COI	KWIA56	1	BC63849_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	GYTAGTTCCRATGATGTTAGCTG	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63849_Pvir_Dia2013_COI	KWIA56	1	BC63849_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	CTTAGTGGCATTAATTCGDAATCC	115.77242
BC63849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19035	WGS84	BC63849	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63849_Rhar_Sim2018_COI	KWIA56	1	BC63849_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63849	Sample	BC63849	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA56	COI	45	BC63849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA56	CTGCTGCTATTGCTCATG	115.77242
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63850_Aamu_Bax2006_COI	KWIA55	1	BC63850_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	GCACAACCGGGATCTTTACTTC	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63850_Pcan_Bot2011_IGS	KWIA55	1	BC63850_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA55	IGS	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	CGTAATCCTCAGTACTGGCTA	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63850_Pper_Dia2013_COI	KWIA55	1	BC63850_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	CTTAGTGGCATTAATTCGDAATCC	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63850_Sspa_Oph2007_COI	KWIA55	1	BC63850_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	GAAGGCCTTCCGATGATC	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63850_Upin_Bot2011_COI	KWIA55	1	BC63850_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	CTTTAATTACAGCGTTTTTATTGTTGT	115.77384
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63851_Cjap_Sim2018_COI	KWIA54	1	BC63851_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63851_Mgig_Bot2012_COI	KWIA54	1	BC63851_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	TCTTATTCGTTGGAGACTTTATAACCCT	115.7731
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63852_Cmae_Bot2011_COI	KWIA53	1	BC63852_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	ATGAACAGTCTATCCTCCTTTAG	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63852_Msal_Bot2012_COI	KWIA53	1	BC63852_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	GYTAGTTCCRATGATGTTAGCTG	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63852_Pvir_Dia2013_COI	KWIA53	1	BC63852_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	CTTAGTGGCATTAATTCGDAATCC	115.77131
BC63852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19102	WGS84	BC63852	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63852_Rhar_Sim2018_COI	KWIA53	1	BC63852_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63852	Sample	BC63852	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA53	COI	45	BC63852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA53	CTGCTGCTATTGCTCATG	115.77131
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63854_Aamu_Bax2006_COI	KWIA51	1	BC63854_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	GCACAACCGGGATCTTTACTTC	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63854_Pcan_Bot2011_IGS	KWIA51	1	BC63854_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA51	IGS	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	CGTAATCCTCAGTACTGGCTA	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63854_Pper_Dia2013_COI	KWIA51	1	BC63854_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	CTTAGTGGCATTAATTCGDAATCC	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63854_Sspa_Oph2007_COI	KWIA51	1	BC63854_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	GAAGGCCTTCCGATGATC	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63854_Upin_Bot2011_COI	KWIA51	1	BC63854_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	CTTTAATTACAGCGTTTTTATTGTTGT	115.76603
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63855_Cjap_Sim2018_COI	KWIA50	1	BC63855_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63855_Mgig_Bot2012_COI	KWIA50	1	BC63855_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	TCTTATTCGTTGGAGACTTTATAACCCT	115.76389
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63856_Cmae_Bot2011_COI	KWIA49	1	BC63856_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	ATGAACAGTCTATCCTCCTTTAG	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63856_Msal_Bot2012_COI	KWIA49	1	BC63856_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	GYTAGTTCCRATGATGTTAGCTG	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63856_Pvir_Dia2013_COI	KWIA49	1	BC63856_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	CTTAGTGGCATTAATTCGDAATCC	115.76479
BC63856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20918	WGS84	BC63856	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63856_Rhar_Sim2018_COI	KWIA49	1	BC63856_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63856	Sample	BC63856	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA49	COI	45	BC63856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA49	CTGCTGCTATTGCTCATG	115.76479
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63858_Aamu_Bax2006_COI	KWIA47	1	BC63858_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	GCACAACCGGGATCTTTACTTC	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63858_Pcan_Bot2011_IGS	KWIA47	1	BC63858_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA47	IGS	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	CGTAATCCTCAGTACTGGCTA	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63858_Pper_Dia2013_COI	KWIA47	1	BC63858_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	CTTAGTGGCATTAATTCGDAATCC	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63858_Sspa_Oph2007_COI	KWIA47	1	BC63858_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	GAAGGCCTTCCGATGATC	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63858_Upin_Bot2011_COI	KWIA47	1	BC63858_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	CTTTAATTACAGCGTTTTTATTGTTGT	115.76541
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63859_Cjap_Sim2018_COI	KWIA46	1	BC63859_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63859_Mgig_Bot2012_COI	KWIA46	1	BC63859_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	TCTTATTCGTTGGAGACTTTATAACCCT	115.7633
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63861_Aamu_Bax2006_COI	KWIA44	1	BC63861_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	GCACAACCGGGATCTTTACTTC	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63861_Pcan_Bot2011_IGS	KWIA44	1	BC63861_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA44	IGS	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	CGTAATCCTCAGTACTGGCTA	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63861_Pper_Dia2013_COI	KWIA44	1	BC63861_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	CTTAGTGGCATTAATTCGDAATCC	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63861_Sspa_Oph2007_COI	KWIA44	1	BC63861_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	GAAGGCCTTCCGATGATC	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63861_Upin_Bot2011_COI	KWIA44	1	BC63861_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	CTTTAATTACAGCGTTTTTATTGTTGT	115.76186
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63862_Cjap_Sim2018_COI	KWIA43	1	BC63862_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63862_Mgig_Bot2012_COI	KWIA43	1	BC63862_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	TCTTATTCGTTGGAGACTTTATAACCCT	115.75889
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63863_Cmae_Bot2011_COI	KWIA42	1	BC63863_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	ATGAACAGTCTATCCTCCTTTAG	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63863_Msal_Bot2012_COI	KWIA42	1	BC63863_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	GYTAGTTCCRATGATGTTAGCTG	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63863_Pvir_Dia2013_COI	KWIA42	1	BC63863_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	CTTAGTGGCATTAATTCGDAATCC	115.75685
BC63863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22905	WGS84	BC63863	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63863_Rhar_Sim2018_COI	KWIA42	1	BC63863_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63863	Sample	BC63863	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA42	COI	45	BC63863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA42	CTGCTGCTATTGCTCATG	115.75685
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63865_Aamu_Bax2006_COI	KWIA40	1	BC63865_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	GCACAACCGGGATCTTTACTTC	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63865_Pcan_Bot2011_IGS	KWIA40	1	BC63865_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA40	IGS	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	CGTAATCCTCAGTACTGGCTA	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63865_Pper_Dia2013_COI	KWIA40	1	BC63865_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	CTTAGTGGCATTAATTCGDAATCC	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63865_Sspa_Oph2007_COI	KWIA40	1	BC63865_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	GAAGGCCTTCCGATGATC	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63865_Upin_Bot2011_COI	KWIA40	1	BC63865_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	CTTTAATTACAGCGTTTTTATTGTTGT	115.75719
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63866_Cjap_Sim2018_COI	KWIA39	1	BC63866_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63866_Mgig_Bot2012_COI	KWIA39	1	BC63866_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	TCTTATTCGTTGGAGACTTTATAACCCT	115.75548
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63867_Cmae_Bot2011_COI	KWIA38	1	BC63867_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	ATGAACAGTCTATCCTCCTTTAG	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63867_Msal_Bot2012_COI	KWIA38	1	BC63867_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	GYTAGTTCCRATGATGTTAGCTG	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63867_Pvir_Dia2013_COI	KWIA38	1	BC63867_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	CTTAGTGGCATTAATTCGDAATCC	115.75354
BC63867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2293	WGS84	BC63867	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63867_Rhar_Sim2018_COI	KWIA38	1	BC63867_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63867	Sample	BC63867	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA38	COI	45	BC63867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA38	CTGCTGCTATTGCTCATG	115.75354
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63869_Aamu_Bax2006_COI	KWIA36	1	BC63869_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	GCACAACCGGGATCTTTACTTC	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63869_Pcan_Bot2011_IGS	KWIA36	1	BC63869_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA36	IGS	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	CGTAATCCTCAGTACTGGCTA	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63869_Pper_Dia2013_COI	KWIA36	1	BC63869_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	CTTAGTGGCATTAATTCGDAATCC	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63869_Sspa_Oph2007_COI	KWIA36	1	BC63869_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	GAAGGCCTTCCGATGATC	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63869_Upin_Bot2011_COI	KWIA36	1	BC63869_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	CTTTAATTACAGCGTTTTTATTGTTGT	115.75422
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63870_Cmae_Bot2011_COI	KWIA35	1	BC63870_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	ATGAACAGTCTATCCTCCTTTAG	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63870_Msal_Bot2012_COI	KWIA35	1	BC63870_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	GYTAGTTCCRATGATGTTAGCTG	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63870_Pvir_Dia2013_COI	KWIA35	1	BC63870_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	CTTAGTGGCATTAATTCGDAATCC	115.757
BC63870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23261	WGS84	BC63870	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63870_Rhar_Sim2018_COI	KWIA35	1	BC63870_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63870	Sample	BC63870	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA35	COI	45	BC63870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA35	CTGCTGCTATTGCTCATG	115.757
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63872_Aamu_Bax2006_COI	KWIA33	1	BC63872_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	GCACAACCGGGATCTTTACTTC	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63872_Pcan_Bot2011_IGS	KWIA33	1	BC63872_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA33	IGS	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	CGTAATCCTCAGTACTGGCTA	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63872_Pper_Dia2013_COI	KWIA33	1	BC63872_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	CTTAGTGGCATTAATTCGDAATCC	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63872_Sspa_Oph2007_COI	KWIA33	1	BC63872_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	GAAGGCCTTCCGATGATC	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63872_Upin_Bot2011_COI	KWIA33	1	BC63872_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	CTTTAATTACAGCGTTTTTATTGTTGT	115.75344
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63873_Cjap_Sim2018_COI	KWIA32	1	BC63873_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63873_Mgig_Bot2012_COI	KWIA32	1	BC63873_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	TCTTATTCGTTGGAGACTTTATAACCCT	115.75404
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63874_Cmae_Bot2011_COI	KWIA31	1	BC63874_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	ATGAACAGTCTATCCTCCTTTAG	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63874_Msal_Bot2012_COI	KWIA31	1	BC63874_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	GYTAGTTCCRATGATGTTAGCTG	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63874_Pvir_Dia2013_COI	KWIA31	1	BC63874_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	CTTAGTGGCATTAATTCGDAATCC	115.7577
BC63874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2365	WGS84	BC63874	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63874_Rhar_Sim2018_COI	KWIA31	1	BC63874_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63874	Sample	BC63874	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA31	COI	45	BC63874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA31	CTGCTGCTATTGCTCATG	115.7577
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63876_Aamu_Bax2006_COI	KWIA29	1	BC63876_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	GCACAACCGGGATCTTTACTTC	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63876_Pcan_Bot2011_IGS	KWIA29	1	BC63876_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA29	IGS	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	CGTAATCCTCAGTACTGGCTA	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63876_Pper_Dia2013_COI	KWIA29	1	BC63876_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	CTTAGTGGCATTAATTCGDAATCC	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63876_Sspa_Oph2007_COI	KWIA29	1	BC63876_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	GAAGGCCTTCCGATGATC	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63876_Upin_Bot2011_COI	KWIA29	1	BC63876_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	CTTTAATTACAGCGTTTTTATTGTTGT	115.75383
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63877_Cjap_Sim2018_COI	KWIA28	1	BC63877_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63877_Mgig_Bot2012_COI	KWIA28	1	BC63877_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	TCTTATTCGTTGGAGACTTTATAACCCT	115.75828
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63878_Cmae_Bot2011_COI	KWIA27	1	BC63878_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	ATGAACAGTCTATCCTCCTTTAG	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63878_Msal_Bot2012_COI	KWIA27	1	BC63878_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	GYTAGTTCCRATGATGTTAGCTG	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63878_Pvir_Dia2013_COI	KWIA27	1	BC63878_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	CTTAGTGGCATTAATTCGDAATCC	115.7564
BC63878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23766	WGS84	BC63878	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63878_Rhar_Sim2018_COI	KWIA27	1	BC63878_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63878	Sample	BC63878	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA27	COI	45	BC63878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA27	CTGCTGCTATTGCTCATG	115.7564
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63880_Cjap_Sim2018_COI	KWIA25	1	BC63880_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63880_Mgig_Bot2012_COI	KWIA25	1	BC63880_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	TCTTATTCGTTGGAGACTTTATAACCCT	115.75253
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63881_Cmae_Bot2011_COI	KWIA24	1	BC63881_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	ATGAACAGTCTATCCTCCTTTAG	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63881_Msal_Bot2012_COI	KWIA24	1	BC63881_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	GYTAGTTCCRATGATGTTAGCTG	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63881_Pvir_Dia2013_COI	KWIA24	1	BC63881_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	CTTAGTGGCATTAATTCGDAATCC	115.75717
BC63881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23932	WGS84	BC63881	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63881_Rhar_Sim2018_COI	KWIA24	1	BC63881_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63881	Sample	BC63881	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA24	COI	45	BC63881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA24	CTGCTGCTATTGCTCATG	115.75717
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63883_Aamu_Bax2006_COI	KWIA22	1	BC63883_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	GCACAACCGGGATCTTTACTTC	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63883_Pcan_Bot2011_IGS	KWIA22	1	BC63883_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA22	IGS	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	CGTAATCCTCAGTACTGGCTA	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63883_Pper_Dia2013_COI	KWIA22	1	BC63883_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	CTTAGTGGCATTAATTCGDAATCC	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63883_Sspa_Oph2007_COI	KWIA22	1	BC63883_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	GAAGGCCTTCCGATGATC	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63883_Upin_Bot2011_COI	KWIA22	1	BC63883_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	CTTTAATTACAGCGTTTTTATTGTTGT	115.7579
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63884_Cjap_Sim2018_COI	KWIA21	1	BC63884_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63884_Mgig_Bot2012_COI	KWIA21	1	BC63884_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	TCTTATTCGTTGGAGACTTTATAACCCT	115.75653
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63885_Cmae_Bot2011_COI	KWIA20	1	BC63885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	ATGAACAGTCTATCCTCCTTTAG	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63885_Msal_Bot2012_COI	KWIA20	1	BC63885_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	GYTAGTTCCRATGATGTTAGCTG	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63885_Pvir_Dia2013_COI	KWIA20	1	BC63885_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	CTTAGTGGCATTAATTCGDAATCC	115.75401
BC63885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24015	WGS84	BC63885	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63885_Rhar_Sim2018_COI	KWIA20	1	BC63885_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63885	Sample	BC63885	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA20	COI	45	BC63885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA20	CTGCTGCTATTGCTCATG	115.75401
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63887_Aamu_Bax2006_COI	KWIA18	1	BC63887_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	GCACAACCGGGATCTTTACTTC	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63887_Pcan_Bot2011_IGS	KWIA18	1	BC63887_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA18	IGS	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	CGTAATCCTCAGTACTGGCTA	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63887_Pper_Dia2013_COI	KWIA18	1	BC63887_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	CTTAGTGGCATTAATTCGDAATCC	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63887_Sspa_Oph2007_COI	KWIA18	1	BC63887_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	GAAGGCCTTCCGATGATC	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63887_Upin_Bot2011_COI	KWIA18	1	BC63887_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	CTTTAATTACAGCGTTTTTATTGTTGT	115.757
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63888_Cjap_Sim2018_COI	KWIA17	1	BC63888_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63888_Mgig_Bot2012_COI	KWIA17	1	BC63888_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	TCTTATTCGTTGGAGACTTTATAACCCT	115.75289
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63889_Cmae_Bot2011_COI	KWIA16	1	BC63889_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	ATGAACAGTCTATCCTCCTTTAG	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63889_Msal_Bot2012_COI	KWIA16	1	BC63889_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	GYTAGTTCCRATGATGTTAGCTG	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63889_Pvir_Dia2013_COI	KWIA16	1	BC63889_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	CTTAGTGGCATTAATTCGDAATCC	115.75755
BC63889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24382	WGS84	BC63889	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63889_Rhar_Sim2018_COI	KWIA16	1	BC63889_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63889	Sample	BC63889	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA16	COI	45	BC63889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA16	CTGCTGCTATTGCTCATG	115.75755
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63890_Aamu_Bax2006_COI	KWIA15	1	BC63890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	GCACAACCGGGATCTTTACTTC	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63890_Pcan_Bot2011_IGS	KWIA15	1	BC63890_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA15	IGS	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	CGTAATCCTCAGTACTGGCTA	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63890_Pper_Dia2013_COI	KWIA15	1	BC63890_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	CTTAGTGGCATTAATTCGDAATCC	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63890_Sspa_Oph2007_COI	KWIA15	1	BC63890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	GAAGGCCTTCCGATGATC	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63890_Upin_Bot2011_COI	KWIA15	1	BC63890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	CTTTAATTACAGCGTTTTTATTGTTGT	115.75557
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63891_Cjap_Sim2018_COI	KWIA14	1	BC63891_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63891_Mgig_Bot2012_COI	KWIA14	1	BC63891_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	TCTTATTCGTTGGAGACTTTATAACCCT	115.75365
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63892_Cmae_Bot2011_COI	KWIA13	1	BC63892_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	ATGAACAGTCTATCCTCCTTTAG	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63892_Msal_Bot2012_COI	KWIA13	1	BC63892_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	GYTAGTTCCRATGATGTTAGCTG	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63892_Pvir_Dia2013_COI	KWIA13	1	BC63892_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	CTTAGTGGCATTAATTCGDAATCC	115.75187
BC63892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24329	WGS84	BC63892	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63892_Rhar_Sim2018_COI	KWIA13	1	BC63892_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63892	Sample	BC63892	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA13	COI	45	BC63892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA13	CTGCTGCTATTGCTCATG	115.75187
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63894_Aamu_Bax2006_COI	KWIA11	1	BC63894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	GCACAACCGGGATCTTTACTTC	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63894_Pcan_Bot2011_IGS	KWIA11	1	BC63894_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA11	IGS	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	CGTAATCCTCAGTACTGGCTA	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63894_Pper_Dia2013_COI	KWIA11	1	BC63894_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	CTTAGTGGCATTAATTCGDAATCC	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63894_Sspa_Oph2007_COI	KWIA11	1	BC63894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	GAAGGCCTTCCGATGATC	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63894_Upin_Bot2011_COI	KWIA11	1	BC63894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	CTTTAATTACAGCGTTTTTATTGTTGT	115.74442
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63895_Cjap_Sim2018_COI	KWIA10	1	BC63895_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63895_Mgig_Bot2012_COI	KWIA10	1	BC63895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	TCTTATTCGTTGGAGACTTTATAACCCT	115.74309
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63896_Cmae_Bot2011_COI	KWIA9	1	BC63896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	ATGAACAGTCTATCCTCCTTTAG	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63896_Msal_Bot2012_COI	KWIA9	1	BC63896_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	GYTAGTTCCRATGATGTTAGCTG	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63896_Pvir_Dia2013_COI	KWIA9	1	BC63896_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	CTTAGTGGCATTAATTCGDAATCC	115.74164
BC63896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25269	WGS84	BC63896	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63896_Rhar_Sim2018_COI	KWIA9	1	BC63896_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63896	Sample	BC63896	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA9	COI	45	BC63896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA9	CTGCTGCTATTGCTCATG	115.74164
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63898_Aamu_Bax2006_COI	KWIA7	1	BC63898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	GCACAACCGGGATCTTTACTTC	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63898_Pcan_Bot2011_IGS	KWIA7	1	BC63898_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA7	IGS	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	CGTAATCCTCAGTACTGGCTA	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63898_Pper_Dia2013_COI	KWIA7	1	BC63898_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	CTTAGTGGCATTAATTCGDAATCC	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63898_Sspa_Oph2007_COI	KWIA7	1	BC63898_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	GAAGGCCTTCCGATGATC	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63898_Upin_Bot2011_COI	KWIA7	1	BC63898_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	CTTTAATTACAGCGTTTTTATTGTTGT	115.74208
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63899_Cjap_Sim2018_COI	KWIA6	1	BC63899_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63899_Mgig_Bot2012_COI	KWIA6	1	BC63899_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	TCTTATTCGTTGGAGACTTTATAACCCT	115.74095
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63900_Cmae_Bot2011_COI	KWIA5	1	BC63900_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	ATGAACAGTCTATCCTCCTTTAG	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63900_Msal_Bot2012_COI	KWIA5	1	BC63900_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	GYTAGTTCCRATGATGTTAGCTG	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63900_Pvir_Dia2013_COI	KWIA5	1	BC63900_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	CTTAGTGGCATTAATTCGDAATCC	115.74475
BC63900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2547	WGS84	BC63900	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63900_Rhar_Sim2018_COI	KWIA5	1	BC63900_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63900	Sample	BC63900	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA5	COI	45	BC63900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA5	CTGCTGCTATTGCTCATG	115.74475
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63902_Aamu_Bax2006_COI	KWIA3	1	BC63902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	GCACAACCGGGATCTTTACTTC	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63902_Pcan_Bot2011_IGS	KWIA3	1	BC63902_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA3	IGS	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	CGTAATCCTCAGTACTGGCTA	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63902_Pper_Dia2013_COI	KWIA3	1	BC63902_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	CTTAGTGGCATTAATTCGDAATCC	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63902_Sspa_Oph2007_COI	KWIA3	1	BC63902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	GAAGGCCTTCCGATGATC	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63902_Upin_Bot2011_COI	KWIA3	1	BC63902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	CTTTAATTACAGCGTTTTTATTGTTGT	115.74126
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63903_Cjap_Sim2018_COI	KWIA2	1	BC63903_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63903_Mgig_Bot2012_COI	KWIA2	1	BC63903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	TCTTATTCGTTGGAGACTTTATAACCCT	115.74164
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63904_Cmae_Bot2011_COI	KWIA1	1	BC63904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	ATGAACAGTCTATCCTCCTTTAG	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63904_Msal_Bot2012_COI	KWIA1	1	BC63904_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	GYTAGTTCCRATGATGTTAGCTG	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63904_Pvir_Dia2013_COI	KWIA1	1	BC63904_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	CTTAGTGGCATTAATTCGDAATCC	115.74031
BC63904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25579	WGS84	BC63904	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63904_Rhar_Sim2018_COI	KWIA1	1	BC63904_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63904	Sample	BC63904	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA1	COI	45	BC63904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA1	CTGCTGCTATTGCTCATG	115.74031
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63906_Aamu_Bax2006_COI	FREA33	1	BC63906_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	GCACAACCGGGATCTTTACTTC	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63906_Pcan_Bot2011_IGS	FREA33	1	BC63906_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA33	IGS	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	CGTAATCCTCAGTACTGGCTA	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63906_Pper_Dia2013_COI	FREA33	1	BC63906_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Pper_Dia2013_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	CTTAGTGGCATTAATTCGDAATCC	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63906_Sspa_Oph2007_COI	FREA33	1	BC63906_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	GAAGGCCTTCCGATGATC	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63906_Upin_Bot2011_COI	FREA33	1	BC63906_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Upin_Bot2011_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	CTTTAATTACAGCGTTTTTATTGTTGT	115.75228
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63907_Cjap_Sim2018_COI	FREA5	1	BC63907_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63907_Esin_And2018_Cyb	FREA5	1	BC63907_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Esin_And2018_Cyb	FREA5	Cytb	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	ACCCCTCCTCATATCCAACCA	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63907_Mgig_Bot2012_COI	FREA5	1	BC63907_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	TCTTATTCGTTGGAGACTTTATAACCCT	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63907_Mjap_Gib2022_COI	FREA5	1	BC63907_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	AATTTAATTGTGACTAGGCATGGTCTG	115.74059
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63908_Cmae_Bot2011_COI	FREA10	1	BC63908_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	ATGAACAGTCTATCCTCCTTTAG	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63908_Hsan_Wil2021_COI	FREA10	1	BC63908_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	CCTGGGCCGGTATAGTAGGT	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63908_Mros_Gib2022_COI	FREA10	1	BC63908_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Mros_Gib2022_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63908_Msal_Bot2012_COI	FREA10	1	BC63908_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Msal_Bot2012_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	GYTAGTTCCRATGATGTTAGCTG	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63908_Pvir_Dia2013_COI	FREA10	1	BC63908_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	CTTAGTGGCATTAATTCGDAATCC	115.74273
BC63908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04847	WGS84	BC63908	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63908_Rhar_Sim2018_COI	FREA10	1	BC63908_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63908	Sample	BC63908	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA10	COI	45	BC63908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA10	CTGCTGCTATTGCTCATG	115.74273
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63910_Cjap_Sim2018_COI	FREA20	1	BC63910_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63910_Esin_And2018_Cyb	FREA20	1	BC63910_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Esin_And2018_Cyb	FREA20	Cytb	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	ACCCCTCCTCATATCCAACCA	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63910_Mgig_Bot2012_COI	FREA20	1	BC63910_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	TCTTATTCGTTGGAGACTTTATAACCCT	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63910_Mjap_Gib2022_COI	FREA20	1	BC63910_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	AATTTAATTGTGACTAGGCATGGTCTG	115.74761
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63911_Cmae_Bot2011_COI	FREA25	1	BC63911_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	ATGAACAGTCTATCCTCCTTTAG	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63911_Msal_Bot2012_COI	FREA25	1	BC63911_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Msal_Bot2012_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	GYTAGTTCCRATGATGTTAGCTG	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63911_Pvir_Dia2013_COI	FREA25	1	BC63911_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	CTTAGTGGCATTAATTCGDAATCC	115.74809
BC63911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04347	WGS84	BC63911	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63911_Rhar_Sim2018_COI	FREA25	1	BC63911_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63911	Sample	BC63911	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA25	COI	45	BC63911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA25	CTGCTGCTATTGCTCATG	115.74809
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63913_Aamu_Bax2006_COI	FREA1	1	BC63913_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	GCACAACCGGGATCTTTACTTC	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63913_Pcan_Bot2011_IGS	FREA1	1	BC63913_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA1	IGS	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	CGTAATCCTCAGTACTGGCTA	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63913_Pper_Dia2013_COI	FREA1	1	BC63913_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Pper_Dia2013_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	CTTAGTGGCATTAATTCGDAATCC	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63913_Sspa_Oph2007_COI	FREA1	1	BC63913_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	GAAGGCCTTCCGATGATC	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63913_Upin_Bot2011_COI	FREA1	1	BC63913_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Upin_Bot2011_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	CTTTAATTACAGCGTTTTTATTGTTGT	115.73752
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63914_Cjap_Sim2018_COI	FREA2	1	BC63914_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63914_Esin_And2018_Cyb	FREA2	1	BC63914_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Esin_And2018_Cyb	FREA2	Cytb	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	ACCCCTCCTCATATCCAACCA	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63914_Mgig_Bot2012_COI	FREA2	1	BC63914_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	TCTTATTCGTTGGAGACTTTATAACCCT	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63914_Mjap_Gib2022_COI	FREA2	1	BC63914_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	AATTTAATTGTGACTAGGCATGGTCTG	115.73877
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63915_Cmae_Bot2011_COI	FREA3	1	BC63915_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	ATGAACAGTCTATCCTCCTTTAG	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63915_Hsan_Wil2021_COI	FREA3	1	BC63915_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	CCTGGGCCGGTATAGTAGGT	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63915_Mros_Gib2022_COI	FREA3	1	BC63915_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Mros_Gib2022_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63915_Msal_Bot2012_COI	FREA3	1	BC63915_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Msal_Bot2012_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	GYTAGTTCCRATGATGTTAGCTG	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63915_Pvir_Dia2013_COI	FREA3	1	BC63915_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	CTTAGTGGCATTAATTCGDAATCC	115.73986
BC63915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05213	WGS84	BC63915	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63915_Rhar_Sim2018_COI	FREA3	1	BC63915_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63915	Sample	BC63915	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA3	COI	45	BC63915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA3	CTGCTGCTATTGCTCATG	115.73986
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63917_Aamu_Bax2006_COI	FREA6	1	BC63917_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	GCACAACCGGGATCTTTACTTC	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63917_Pcan_Bot2011_IGS	FREA6	1	BC63917_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA6	IGS	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	CGTAATCCTCAGTACTGGCTA	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63917_Pper_Dia2013_COI	FREA6	1	BC63917_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Pper_Dia2013_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	CTTAGTGGCATTAATTCGDAATCC	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63917_Sspa_Oph2007_COI	FREA6	1	BC63917_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	GAAGGCCTTCCGATGATC	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63917_Upin_Bot2011_COI	FREA6	1	BC63917_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Upin_Bot2011_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	CTTTAATTACAGCGTTTTTATTGTTGT	115.74243
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63918_Cjap_Sim2018_COI	FREA7	1	BC63918_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63918_Esin_And2018_Cyb	FREA7	1	BC63918_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Esin_And2018_Cyb	FREA7	Cytb	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	ACCCCTCCTCATATCCAACCA	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63918_Mgig_Bot2012_COI	FREA7	1	BC63918_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	TCTTATTCGTTGGAGACTTTATAACCCT	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63918_Mjap_Gib2022_COI	FREA7	1	BC63918_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	AATTTAATTGTGACTAGGCATGGTCTG	115.74189
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63919_Cmae_Bot2011_COI	FREA8	1	BC63919_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	ATGAACAGTCTATCCTCCTTTAG	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63919_Hsan_Wil2021_COI	FREA8	1	BC63919_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	CCTGGGCCGGTATAGTAGGT	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63919_Mros_Gib2022_COI	FREA8	1	BC63919_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Mros_Gib2022_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63919_Msal_Bot2012_COI	FREA8	1	BC63919_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Msal_Bot2012_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	GYTAGTTCCRATGATGTTAGCTG	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63919_Pvir_Dia2013_COI	FREA8	1	BC63919_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	CTTAGTGGCATTAATTCGDAATCC	115.74336
BC63919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05005	WGS84	BC63919	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63919_Rhar_Sim2018_COI	FREA8	1	BC63919_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63919	Sample	BC63919	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA8	COI	45	BC63919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA8	CTGCTGCTATTGCTCATG	115.74336
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63920_Aamu_Bax2006_COI	FREA9	1	BC63920_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	GCACAACCGGGATCTTTACTTC	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63920_Pcan_Bot2011_IGS	FREA9	1	BC63920_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA9	IGS	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	CGTAATCCTCAGTACTGGCTA	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63920_Pper_Dia2013_COI	FREA9	1	BC63920_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Pper_Dia2013_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	CTTAGTGGCATTAATTCGDAATCC	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63920_Sspa_Oph2007_COI	FREA9	1	BC63920_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	GAAGGCCTTCCGATGATC	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63920_Upin_Bot2011_COI	FREA9	1	BC63920_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Upin_Bot2011_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	CTTTAATTACAGCGTTTTTATTGTTGT	115.74142
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63921_Cjap_Sim2018_COI	FREA11	1	BC63921_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63921_Esin_And2018_Cyb	FREA11	1	BC63921_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Esin_And2018_Cyb	FREA11	Cytb	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	ACCCCTCCTCATATCCAACCA	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63921_Mgig_Bot2012_COI	FREA11	1	BC63921_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	TCTTATTCGTTGGAGACTTTATAACCCT	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63921_Mjap_Gib2022_COI	FREA11	1	BC63921_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	AATTTAATTGTGACTAGGCATGGTCTG	115.74474
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63922_Cmae_Bot2011_COI	FREA12	1	BC63922_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	ATGAACAGTCTATCCTCCTTTAG	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63922_Hsan_Wil2021_COI	FREA12	1	BC63922_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	CCTGGGCCGGTATAGTAGGT	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63922_Mros_Gib2022_COI	FREA12	1	BC63922_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Mros_Gib2022_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63922_Msal_Bot2012_COI	FREA12	1	BC63922_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Msal_Bot2012_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	GYTAGTTCCRATGATGTTAGCTG	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63922_Pvir_Dia2013_COI	FREA12	1	BC63922_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	CTTAGTGGCATTAATTCGDAATCC	115.74231
BC63922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04752	WGS84	BC63922	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63922_Rhar_Sim2018_COI	FREA12	1	BC63922_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63922	Sample	BC63922	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA12	COI	45	BC63922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA12	CTGCTGCTATTGCTCATG	115.74231
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63924_Aamu_Bax2006_COI	FREA14	1	BC63924_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	GCACAACCGGGATCTTTACTTC	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63924_Pcan_Bot2011_IGS	FREA14	1	BC63924_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA14	IGS	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	CGTAATCCTCAGTACTGGCTA	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63924_Pper_Dia2013_COI	FREA14	1	BC63924_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Pper_Dia2013_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	CTTAGTGGCATTAATTCGDAATCC	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63924_Sspa_Oph2007_COI	FREA14	1	BC63924_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	GAAGGCCTTCCGATGATC	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63924_Upin_Bot2011_COI	FREA14	1	BC63924_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Upin_Bot2011_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	CTTTAATTACAGCGTTTTTATTGTTGT	115.74543
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63925_Cjap_Sim2018_COI	FREA16	1	BC63925_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63925_Esin_And2018_Cyb	FREA16	1	BC63925_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Esin_And2018_Cyb	FREA16	Cytb	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	ACCCCTCCTCATATCCAACCA	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63925_Mgig_Bot2012_COI	FREA16	1	BC63925_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	TCTTATTCGTTGGAGACTTTATAACCCT	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63925_Mjap_Gib2022_COI	FREA16	1	BC63925_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	AATTTAATTGTGACTAGGCATGGTCTG	115.74466
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63926_Cmae_Bot2011_COI	FREA21	1	BC63926_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	ATGAACAGTCTATCCTCCTTTAG	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63926_Hsan_Wil2021_COI	FREA21	1	BC63926_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	CCTGGGCCGGTATAGTAGGT	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63926_Mros_Gib2022_COI	FREA21	1	BC63926_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Mros_Gib2022_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63926_Msal_Bot2012_COI	FREA21	1	BC63926_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Msal_Bot2012_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	GYTAGTTCCRATGATGTTAGCTG	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63926_Pvir_Dia2013_COI	FREA21	1	BC63926_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	CTTAGTGGCATTAATTCGDAATCC	115.74536
BC63926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04428	WGS84	BC63926	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63926_Rhar_Sim2018_COI	FREA21	1	BC63926_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63926	Sample	BC63926	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA21	COI	45	BC63926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA21	CTGCTGCTATTGCTCATG	115.74536
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63928_Aamu_Bax2006_COI	FREA19	1	BC63928_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	GCACAACCGGGATCTTTACTTC	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63928_Pcan_Bot2011_IGS	FREA19	1	BC63928_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA19	IGS	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	CGTAATCCTCAGTACTGGCTA	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63928_Pper_Dia2013_COI	FREA19	1	BC63928_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Pper_Dia2013_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	CTTAGTGGCATTAATTCGDAATCC	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63928_Sspa_Oph2007_COI	FREA19	1	BC63928_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	GAAGGCCTTCCGATGATC	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63928_Upin_Bot2011_COI	FREA19	1	BC63928_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Upin_Bot2011_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	CTTTAATTACAGCGTTTTTATTGTTGT	115.74597
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63929_Cjap_Sim2018_COI	FREA17	1	BC63929_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63929_Esin_And2018_Cyb	FREA17	1	BC63929_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Esin_And2018_Cyb	FREA17	Cytb	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	ACCCCTCCTCATATCCAACCA	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63929_Mgig_Bot2012_COI	FREA17	1	BC63929_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	TCTTATTCGTTGGAGACTTTATAACCCT	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63929_Mjap_Gib2022_COI	FREA17	1	BC63929_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	AATTTAATTGTGACTAGGCATGGTCTG	115.74646
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63931_Aamu_Bax2006_COI	FREA23	1	BC63931_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	GCACAACCGGGATCTTTACTTC	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63931_Pcan_Bot2011_IGS	FREA23	1	BC63931_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA23	IGS	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	CGTAATCCTCAGTACTGGCTA	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63931_Pper_Dia2013_COI	FREA23	1	BC63931_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Pper_Dia2013_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	CTTAGTGGCATTAATTCGDAATCC	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63931_Sspa_Oph2007_COI	FREA23	1	BC63931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	GAAGGCCTTCCGATGATC	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63931_Upin_Bot2011_COI	FREA23	1	BC63931_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Upin_Bot2011_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	CTTTAATTACAGCGTTTTTATTGTTGT	115.7487
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63932_Cjap_Sim2018_COI	FREA24	1	BC63932_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63932_Mgig_Bot2012_COI	FREA24	1	BC63932_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	TCTTATTCGTTGGAGACTTTATAACCCT	115.74651
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63933_Cmae_Bot2011_COI	FREA26	1	BC63933_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	ATGAACAGTCTATCCTCCTTTAG	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63933_Msal_Bot2012_COI	FREA26	1	BC63933_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Msal_Bot2012_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	GYTAGTTCCRATGATGTTAGCTG	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63933_Pvir_Dia2013_COI	FREA26	1	BC63933_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	CTTAGTGGCATTAATTCGDAATCC	115.74984
BC63933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04371	WGS84	BC63933	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63933_Rhar_Sim2018_COI	FREA26	1	BC63933_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63933	Sample	BC63933	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA26	COI	45	BC63933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA26	CTGCTGCTATTGCTCATG	115.74984
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63935_Aamu_Bax2006_COI	FREA28	1	BC63935_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	GCACAACCGGGATCTTTACTTC	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63935_Pcan_Bot2011_IGS	FREA28	1	BC63935_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA28	IGS	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	CGTAATCCTCAGTACTGGCTA	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63935_Pper_Dia2013_COI	FREA28	1	BC63935_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Pper_Dia2013_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	CTTAGTGGCATTAATTCGDAATCC	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63935_Sspa_Oph2007_COI	FREA28	1	BC63935_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	GAAGGCCTTCCGATGATC	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63935_Upin_Bot2011_COI	FREA28	1	BC63935_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Upin_Bot2011_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	CTTTAATTACAGCGTTTTTATTGTTGT	115.7493
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63936_Cjap_Sim2018_COI	FREA29	1	BC63936_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63936_Mgig_Bot2012_COI	FREA29	1	BC63936_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	TCTTATTCGTTGGAGACTTTATAACCCT	115.75084
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63937_Cmae_Bot2011_COI	FREA31	1	BC63937_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	ATGAACAGTCTATCCTCCTTTAG	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63937_Msal_Bot2012_COI	FREA31	1	BC63937_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Msal_Bot2012_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	GYTAGTTCCRATGATGTTAGCTG	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63937_Pvir_Dia2013_COI	FREA31	1	BC63937_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	CTTAGTGGCATTAATTCGDAATCC	115.75211
BC63937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04161	WGS84	BC63937	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63937_Rhar_Sim2018_COI	FREA31	1	BC63937_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63937	Sample	BC63937	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA31	COI	45	BC63937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA31	CTGCTGCTATTGCTCATG	115.75211
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63939_Aamu_Bax2006_COI	BUNA22	1	BC63939_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	GCACAACCGGGATCTTTACTTC	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63939_Pcan_Bot2011_IGS	BUNA22	1	BC63939_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA22	IGS	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	CGTAATCCTCAGTACTGGCTA	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63939_Pper_Dia2013_COI	BUNA22	1	BC63939_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	CTTAGTGGCATTAATTCGDAATCC	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63939_Sspa_Oph2007_COI	BUNA22	1	BC63939_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	GAAGGCCTTCCGATGATC	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63939_Upin_Bot2011_COI	BUNA22	1	BC63939_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	CTTTAATTACAGCGTTTTTATTGTTGT	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63939_Vgib_Wil2025_COI	BUNA22	1	BC63939_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	CCCCTCTTTACAAGTTCTTTAGGGT	115.64643
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63940_Cmae_Bot2011_COI	BUNA21	1	BC63940_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	ATGAACAGTCTATCCTCCTTTAG	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63940_Hsan_Wil2021_COI	BUNA21	1	BC63940_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	CCTGGGCCGGTATAGTAGGT	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63940_Mros_Gib2022_COI	BUNA21	1	BC63940_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63940_Msal_Bot2012_COI	BUNA21	1	BC63940_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	GYTAGTTCCRATGATGTTAGCTG	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63940_Pvir_Dia2013_COI	BUNA21	1	BC63940_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	CTTAGTGGCATTAATTCGDAATCC	115.64669
BC63940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30824	WGS84	BC63940	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63940_Rhar_Sim2018_COI	BUNA21	1	BC63940_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63940	Sample	BC63940	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA21	COI	45	BC63940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA21	CTGCTGCTATTGCTCATG	115.64669
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63942_Aamu_Bax2006_COI	BUNA19	1	BC63942_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	GCACAACCGGGATCTTTACTTC	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63942_Pcan_Bot2011_IGS	BUNA19	1	BC63942_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA19	IGS	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	CGTAATCCTCAGTACTGGCTA	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63942_Pper_Dia2013_COI	BUNA19	1	BC63942_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	CTTAGTGGCATTAATTCGDAATCC	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63942_Sspa_Oph2007_COI	BUNA19	1	BC63942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	GAAGGCCTTCCGATGATC	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63942_Upin_Bot2011_COI	BUNA19	1	BC63942_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	CTTTAATTACAGCGTTTTTATTGTTGT	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63942_Vgib_Wil2025_COI	BUNA19	1	BC63942_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	CCCCTCTTTACAAGTTCTTTAGGGT	115.64384
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63943_Asen_Wil2025_COI	BUNA18	1	BC63943_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	GTTGTTGTTACAACACATGCTCTTTTG	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63943_Cjap_Sim2018_COI	BUNA18	1	BC63943_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63943_Esin_And2018_Cyb	BUNA18	1	BC63943_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA18	Cytb	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	ACCCCTCCTCATATCCAACCA	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63943_Mgig_Bot2012_COI	BUNA18	1	BC63943_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	TCTTATTCGTTGGAGACTTTATAACCCT	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63943_Mjap_Gib2022_COI	BUNA18	1	BC63943_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	AATTTAATTGTGACTAGGCATGGTCTG	115.64241
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63944_Cmae_Bot2011_COI	BUNA17	1	BC63944_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	ATGAACAGTCTATCCTCCTTTAG	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63944_Hsan_Wil2021_COI	BUNA17	1	BC63944_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	CCTGGGCCGGTATAGTAGGT	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63944_Mros_Gib2022_COI	BUNA17	1	BC63944_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63944_Msal_Bot2012_COI	BUNA17	1	BC63944_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	GYTAGTTCCRATGATGTTAGCTG	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63944_Pvir_Dia2013_COI	BUNA17	1	BC63944_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	CTTAGTGGCATTAATTCGDAATCC	115.66307
BC63944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32071	WGS84	BC63944	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63944_Rhar_Sim2018_COI	BUNA17	1	BC63944_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63944	Sample	BC63944	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA17	COI	45	BC63944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA17	CTGCTGCTATTGCTCATG	115.66307
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63946_Aamu_Bax2006_COI	BUNA15	1	BC63946_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	GCACAACCGGGATCTTTACTTC	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63946_Pcan_Bot2011_IGS	BUNA15	1	BC63946_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA15	IGS	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	CGTAATCCTCAGTACTGGCTA	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63946_Pper_Dia2013_COI	BUNA15	1	BC63946_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	CTTAGTGGCATTAATTCGDAATCC	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63946_Sspa_Oph2007_COI	BUNA15	1	BC63946_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	GAAGGCCTTCCGATGATC	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63946_Upin_Bot2011_COI	BUNA15	1	BC63946_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	CTTTAATTACAGCGTTTTTATTGTTGT	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63946_Vgib_Wil2025_COI	BUNA15	1	BC63946_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	CCCCTCTTTACAAGTTCTTTAGGGT	115.66542
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63947_Asen_Wil2025_COI	BUNA14	1	BC63947_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	GTTGTTGTTACAACACATGCTCTTTTG	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63947_Cjap_Sim2018_COI	BUNA14	1	BC63947_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63947_Esin_And2018_Cyb	BUNA14	1	BC63947_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA14	Cytb	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	ACCCCTCCTCATATCCAACCA	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63947_Mgig_Bot2012_COI	BUNA14	1	BC63947_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	TCTTATTCGTTGGAGACTTTATAACCCT	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63947_Mjap_Gib2022_COI	BUNA14	1	BC63947_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	AATTTAATTGTGACTAGGCATGGTCTG	115.66383
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63948_Cmae_Bot2011_COI	BUNA13	1	BC63948_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	ATGAACAGTCTATCCTCCTTTAG	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63948_Hsan_Wil2021_COI	BUNA13	1	BC63948_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	CCTGGGCCGGTATAGTAGGT	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63948_Mros_Gib2022_COI	BUNA13	1	BC63948_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63948_Msal_Bot2012_COI	BUNA13	1	BC63948_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	GYTAGTTCCRATGATGTTAGCTG	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63948_Pvir_Dia2013_COI	BUNA13	1	BC63948_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	CTTAGTGGCATTAATTCGDAATCC	115.66214
BC63948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32178	WGS84	BC63948	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63948_Rhar_Sim2018_COI	BUNA13	1	BC63948_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63948	Sample	BC63948	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA13	COI	45	BC63948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA13	CTGCTGCTATTGCTCATG	115.66214
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63950_Asen_Wil2025_COI	BUNA11	1	BC63950_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	GTTGTTGTTACAACACATGCTCTTTTG	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63950_Cjap_Sim2018_COI	BUNA11	1	BC63950_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63950_Esin_And2018_Cyb	BUNA11	1	BC63950_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA11	Cytb	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	ACCCCTCCTCATATCCAACCA	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63950_Mgig_Bot2012_COI	BUNA11	1	BC63950_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	TCTTATTCGTTGGAGACTTTATAACCCT	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63950_Mjap_Gib2022_COI	BUNA11	1	BC63950_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	AATTTAATTGTGACTAGGCATGGTCTG	115.65884
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63951_Cmae_Bot2011_COI	BUNA10	1	BC63951_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	ATGAACAGTCTATCCTCCTTTAG	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63951_Hsan_Wil2021_COI	BUNA10	1	BC63951_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	CCTGGGCCGGTATAGTAGGT	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63951_Mros_Gib2022_COI	BUNA10	1	BC63951_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63951_Msal_Bot2012_COI	BUNA10	1	BC63951_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	GYTAGTTCCRATGATGTTAGCTG	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63951_Pvir_Dia2013_COI	BUNA10	1	BC63951_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	CTTAGTGGCATTAATTCGDAATCC	115.66622
BC63951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32382	WGS84	BC63951	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63951_Rhar_Sim2018_COI	BUNA10	1	BC63951_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63951	Sample	BC63951	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA10	COI	45	BC63951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA10	CTGCTGCTATTGCTCATG	115.66622
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63953_Aamu_Bax2006_COI	BUNA8	1	BC63953_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	GCACAACCGGGATCTTTACTTC	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63953_Pcan_Bot2011_IGS	BUNA8	1	BC63953_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA8	IGS	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	CGTAATCCTCAGTACTGGCTA	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63953_Pper_Dia2013_COI	BUNA8	1	BC63953_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	CTTAGTGGCATTAATTCGDAATCC	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63953_Sspa_Oph2007_COI	BUNA8	1	BC63953_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	GAAGGCCTTCCGATGATC	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63953_Upin_Bot2011_COI	BUNA8	1	BC63953_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	CTTTAATTACAGCGTTTTTATTGTTGT	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63953_Vgib_Wil2025_COI	BUNA8	1	BC63953_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	CCCCTCTTTACAAGTTCTTTAGGGT	115.66291
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63954_Asen_Wil2025_COI	BUNA7	1	BC63954_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	GTTGTTGTTACAACACATGCTCTTTTG	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63954_Cjap_Sim2018_COI	BUNA7	1	BC63954_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63954_Esin_And2018_Cyb	BUNA7	1	BC63954_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA7	Cytb	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	ACCCCTCCTCATATCCAACCA	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63954_Mgig_Bot2012_COI	BUNA7	1	BC63954_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	TCTTATTCGTTGGAGACTTTATAACCCT	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63954_Mjap_Gib2022_COI	BUNA7	1	BC63954_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	AATTTAATTGTGACTAGGCATGGTCTG	115.66124
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63955_Cmae_Bot2011_COI	BUNA6	1	BC63955_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	ATGAACAGTCTATCCTCCTTTAG	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63955_Hsan_Wil2021_COI	BUNA6	1	BC63955_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	CCTGGGCCGGTATAGTAGGT	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63955_Mros_Gib2022_COI	BUNA6	1	BC63955_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63955_Msal_Bot2012_COI	BUNA6	1	BC63955_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	GYTAGTTCCRATGATGTTAGCTG	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63955_Pvir_Dia2013_COI	BUNA6	1	BC63955_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	CTTAGTGGCATTAATTCGDAATCC	115.6689
BC63955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32526	WGS84	BC63955	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63955_Rhar_Sim2018_COI	BUNA6	1	BC63955_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63955	Sample	BC63955	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA6	COI	45	BC63955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA6	CTGCTGCTATTGCTCATG	115.6689
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63957_Aamu_Bax2006_COI	BUNA4	1	BC63957_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	GCACAACCGGGATCTTTACTTC	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63957_Pcan_Bot2011_IGS	BUNA4	1	BC63957_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA4	IGS	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	CGTAATCCTCAGTACTGGCTA	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63957_Pper_Dia2013_COI	BUNA4	1	BC63957_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	CTTAGTGGCATTAATTCGDAATCC	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63957_Sspa_Oph2007_COI	BUNA4	1	BC63957_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	GAAGGCCTTCCGATGATC	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63957_Upin_Bot2011_COI	BUNA4	1	BC63957_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	CTTTAATTACAGCGTTTTTATTGTTGT	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63957_Vgib_Wil2025_COI	BUNA4	1	BC63957_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	CCCCTCTTTACAAGTTCTTTAGGGT	115.66539
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63958_Asen_Wil2025_COI	BUNA3	1	BC63958_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	GTTGTTGTTACAACACATGCTCTTTTG	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63958_Cjap_Sim2018_COI	BUNA3	1	BC63958_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63958_Esin_And2018_Cyb	BUNA3	1	BC63958_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA3	Cytb	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	ACCCCTCCTCATATCCAACCA	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63958_Mgig_Bot2012_COI	BUNA3	1	BC63958_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	TCTTATTCGTTGGAGACTTTATAACCCT	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63958_Mjap_Gib2022_COI	BUNA3	1	BC63958_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	AATTTAATTGTGACTAGGCATGGTCTG	115.66343
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63959_Cmae_Bot2011_COI	BUNA2	1	BC63959_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	ATGAACAGTCTATCCTCCTTTAG	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63959_Hsan_Wil2021_COI	BUNA2	1	BC63959_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	CCTGGGCCGGTATAGTAGGT	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63959_Mros_Gib2022_COI	BUNA2	1	BC63959_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63959_Msal_Bot2012_COI	BUNA2	1	BC63959_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	GYTAGTTCCRATGATGTTAGCTG	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63959_Pvir_Dia2013_COI	BUNA2	1	BC63959_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	CTTAGTGGCATTAATTCGDAATCC	115.66781
BC63959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32578	WGS84	BC63959	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63959_Rhar_Sim2018_COI	BUNA2	1	BC63959_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63959	Sample	BC63959	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA2	COI	45	BC63959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA2	CTGCTGCTATTGCTCATG	115.66781
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63960_Aamu_Bax2006_COI	BUNA1	1	BC63960_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	GCACAACCGGGATCTTTACTTC	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63960_Pcan_Bot2011_IGS	BUNA1	1	BC63960_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA1	IGS	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	CGTAATCCTCAGTACTGGCTA	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63960_Pper_Dia2013_COI	BUNA1	1	BC63960_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	CTTAGTGGCATTAATTCGDAATCC	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63960_Sspa_Oph2007_COI	BUNA1	1	BC63960_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	GAAGGCCTTCCGATGATC	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63960_Upin_Bot2011_COI	BUNA1	1	BC63960_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	CTTTAATTACAGCGTTTTTATTGTTGT	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63960_Vgib_Wil2025_COI	BUNA1	1	BC63960_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	CCCCTCTTTACAAGTTCTTTAGGGT	115.66645
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63961_Cjap_Sim2018_COI	GERA15	1	BC63961_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63961_Mgig_Bot2012_COI	GERA15	1	BC63961_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	TCTTATTCGTTGGAGACTTTATAACCCT	114.59997
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63962_Cmae_Bot2011_COI	GERA14	1	BC63962_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	ATGAACAGTCTATCCTCCTTTAG	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63962_Msal_Bot2012_COI	GERA14	1	BC63962_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Msal_Bot2012_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	GYTAGTTCCRATGATGTTAGCTG	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63962_Pvir_Dia2013_COI	GERA14	1	BC63962_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	CTTAGTGGCATTAATTCGDAATCC	114.60049
BC63962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77278	WGS84	BC63962	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63962_Rhar_Sim2018_COI	GERA14	1	BC63962_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63962	Sample	BC63962	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA14	COI	45	BC63962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA14	CTGCTGCTATTGCTCATG	114.60049
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63964_Aamu_Bax2006_COI	GERA12	1	BC63964_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	GCACAACCGGGATCTTTACTTC	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63964_Pcan_Bot2011_IGS	GERA12	1	BC63964_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA12	IGS	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	CGTAATCCTCAGTACTGGCTA	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63964_Pper_Dia2013_COI	GERA12	1	BC63964_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Pper_Dia2013_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	CTTAGTGGCATTAATTCGDAATCC	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63964_Sspa_Oph2007_COI	GERA12	1	BC63964_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	GAAGGCCTTCCGATGATC	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63964_Upin_Bot2011_COI	GERA12	1	BC63964_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Upin_Bot2011_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	CTTTAATTACAGCGTTTTTATTGTTGT	114.59739
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63965_Cjap_Sim2018_COI	GERA11	1	BC63965_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63965_Mgig_Bot2012_COI	GERA11	1	BC63965_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	TCTTATTCGTTGGAGACTTTATAACCCT	114.59609
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63966_Cmae_Bot2011_COI	GERA10	1	BC63966_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	ATGAACAGTCTATCCTCCTTTAG	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63966_Msal_Bot2012_COI	GERA10	1	BC63966_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Msal_Bot2012_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	GYTAGTTCCRATGATGTTAGCTG	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63966_Pvir_Dia2013_COI	GERA10	1	BC63966_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	CTTAGTGGCATTAATTCGDAATCC	114.60115
BC63966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77408	WGS84	BC63966	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63966_Rhar_Sim2018_COI	GERA10	1	BC63966_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63966	Sample	BC63966	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA10	COI	45	BC63966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA10	CTGCTGCTATTGCTCATG	114.60115
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63968_Aamu_Bax2006_COI	GERA8	1	BC63968_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	GCACAACCGGGATCTTTACTTC	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63968_Pcan_Bot2011_IGS	GERA8	1	BC63968_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA8	IGS	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	CGTAATCCTCAGTACTGGCTA	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63968_Pper_Dia2013_COI	GERA8	1	BC63968_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Pper_Dia2013_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	CTTAGTGGCATTAATTCGDAATCC	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63968_Sspa_Oph2007_COI	GERA8	1	BC63968_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	GAAGGCCTTCCGATGATC	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63968_Upin_Bot2011_COI	GERA8	1	BC63968_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Upin_Bot2011_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	CTTTAATTACAGCGTTTTTATTGTTGT	114.59813
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63969_Cjap_Sim2018_COI	GERA7	1	BC63969_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63969_Mgig_Bot2012_COI	GERA7	1	BC63969_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	TCTTATTCGTTGGAGACTTTATAACCCT	114.59669
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63971_Aamu_Bax2006_COI	GERA5	1	BC63971_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	GCACAACCGGGATCTTTACTTC	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63971_Pcan_Bot2011_IGS	GERA5	1	BC63971_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA5	IGS	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	CGTAATCCTCAGTACTGGCTA	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63971_Pper_Dia2013_COI	GERA5	1	BC63971_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Pper_Dia2013_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	CTTAGTGGCATTAATTCGDAATCC	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63971_Sspa_Oph2007_COI	GERA5	1	BC63971_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	GAAGGCCTTCCGATGATC	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63971_Upin_Bot2011_COI	GERA5	1	BC63971_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Upin_Bot2011_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	CTTTAATTACAGCGTTTTTATTGTTGT	114.59862
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63972_Cjap_Sim2018_COI	GERA4	1	BC63972_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63972_Mgig_Bot2012_COI	GERA4	1	BC63972_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	TCTTATTCGTTGGAGACTTTATAACCCT	114.59705
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63973_Cmae_Bot2011_COI	GERA3	1	BC63973_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	ATGAACAGTCTATCCTCCTTTAG	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63973_Msal_Bot2012_COI	GERA3	1	BC63973_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Msal_Bot2012_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	GYTAGTTCCRATGATGTTAGCTG	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63973_Pvir_Dia2013_COI	GERA3	1	BC63973_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	CTTAGTGGCATTAATTCGDAATCC	114.59549
BC63973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77453	WGS84	BC63973	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63973_Rhar_Sim2018_COI	GERA3	1	BC63973_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63973	Sample	BC63973	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA3	COI	45	BC63973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA3	CTGCTGCTATTGCTCATG	114.59549
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63975_Aamu_Bax2006_COI	GERA1	1	BC63975_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	GCACAACCGGGATCTTTACTTC	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63975_Pcan_Bot2011_IGS	GERA1	1	BC63975_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA1	IGS	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	CGTAATCCTCAGTACTGGCTA	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63975_Pper_Dia2013_COI	GERA1	1	BC63975_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Pper_Dia2013_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	CTTAGTGGCATTAATTCGDAATCC	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63975_Sspa_Oph2007_COI	GERA1	1	BC63975_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	GAAGGCCTTCCGATGATC	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63975_Upin_Bot2011_COI	GERA1	1	BC63975_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Upin_Bot2011_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	CTTTAATTACAGCGTTTTTATTGTTGT	114.59449
BC64565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8123	WGS84	BC64565	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64565_Aamu_Bax2006_COI	ADEAPC-59	1	BC64565_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64565	Sample	BC64565	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-59	COI	45	BC64565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-59	GCACAACCGGGATCTTTACTTC	138.53897
BC64565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8123	WGS84	BC64565	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64565_Pcan_Bot2011_IGS	ADEAPC-59	1	BC64565_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64565	Sample	BC64565	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-59	IGS	45	BC64565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-59	CGTAATCCTCAGTACTGGCTA	138.53897
BC64565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8123	WGS84	BC64565	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.458	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64565_Sspa_Oph2007_COI	ADEAPC-59	1	BC64565_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64565	Sample	BC64565	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-59	COI	45	BC64565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-59	GAAGGCCTTCCGATGATC	138.53897
BC64565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8123	WGS84	BC64565	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64565_Upin_Bot2011_COI	ADEAPC-59	1	BC64565_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64565	Sample	BC64565	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-59	COI	45	BC64565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-59	CTTTAATTACAGCGTTTTTATTGTTGT	138.53897
BC64566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81333	WGS84	BC64566	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64566_Cjap_Sim2018_COI	ADEAPC-58	1	BC64566_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64566	Sample	BC64566	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-58	COI	45	BC64566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-58	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.53131
BC64566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81333	WGS84	BC64566	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.224	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64566_Mgig_Bot2012_COI	ADEAPC-58	1	BC64566_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64566	Sample	BC64566	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-58	COI	45	BC64566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-58	TCTTATTCGTTGGAGACTTTATAACCCT	138.53131
BC64567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81246	WGS84	BC64567	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64567_Cmae_Bot2011_COI	ADEAPC-57	1	BC64567_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64567	Sample	BC64567	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-57	COI	45	BC64567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-57	ATGAACAGTCTATCCTCCTTTAG	138.52837
BC64569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81915	WGS84	BC64569	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64569_Aamu_Bax2006_COI	ADEAPC-55	1	BC64569_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64569	Sample	BC64569	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-55	COI	45	BC64569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-55	GCACAACCGGGATCTTTACTTC	138.51385
BC64569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81915	WGS84	BC64569	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64569_Pcan_Bot2011_IGS	ADEAPC-55	1	BC64569_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64569	Sample	BC64569	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-55	IGS	45	BC64569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-55	CGTAATCCTCAGTACTGGCTA	138.51385
BC64569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81915	WGS84	BC64569	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.163	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64569_Sspa_Oph2007_COI	ADEAPC-55	1	BC64569_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64569	Sample	BC64569	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-55	COI	45	BC64569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-55	GAAGGCCTTCCGATGATC	138.51385
BC64569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81915	WGS84	BC64569	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64569_Upin_Bot2011_COI	ADEAPC-55	1	BC64569_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64569	Sample	BC64569	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-55	COI	45	BC64569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-55	CTTTAATTACAGCGTTTTTATTGTTGT	138.51385
BC64570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83039	WGS84	BC64570	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64570_Cmae_Bot2011_COI	ADEAPC-54	1	BC64570_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64570	Sample	BC64570	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-54	COI	45	BC64570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-54	ATGAACAGTCTATCCTCCTTTAG	138.51097
BC64572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83034	WGS84	BC64572	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64572_Aamu_Bax2006_COI	ADEAPC-52	1	BC64572_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64572	Sample	BC64572	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-52	COI	45	BC64572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-52	GCACAACCGGGATCTTTACTTC	138.51084
BC64572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83034	WGS84	BC64572	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64572_Pcan_Bot2011_IGS	ADEAPC-52	1	BC64572_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64572	Sample	BC64572	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-52	IGS	45	BC64572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-52	CGTAATCCTCAGTACTGGCTA	138.51084
BC64572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83034	WGS84	BC64572	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.114	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64572_Sspa_Oph2007_COI	ADEAPC-52	1	BC64572_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64572	Sample	BC64572	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-52	COI	45	BC64572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-52	GAAGGCCTTCCGATGATC	138.51084
BC64572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83034	WGS84	BC64572	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64572_Upin_Bot2011_COI	ADEAPC-52	1	BC64572_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64572	Sample	BC64572	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-52	COI	45	BC64572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-52	CTTTAATTACAGCGTTTTTATTGTTGT	138.51084
BC64573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83073	WGS84	BC64573	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64573_Cjap_Sim2018_COI	ADEAPC-51	1	BC64573_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64573	Sample	BC64573	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-51	COI	45	BC64573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-51	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51131
BC64573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83073	WGS84	BC64573	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.307	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64573_Mgig_Bot2012_COI	ADEAPC-51	1	BC64573_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64573	Sample	BC64573	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-51	COI	45	BC64573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-51	TCTTATTCGTTGGAGACTTTATAACCCT	138.51131
BC64574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83719	WGS84	BC64574	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64574_Cmae_Bot2011_COI	ADEAPC-50	1	BC64574_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64574	Sample	BC64574	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-50	COI	45	BC64574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-50	ATGAACAGTCTATCCTCCTTTAG	138.5105
BC64576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83724	WGS84	BC64576	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64576_Aamu_Bax2006_COI	ADEAPC-48	1	BC64576_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64576	Sample	BC64576	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-48	COI	45	BC64576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-48	GCACAACCGGGATCTTTACTTC	138.51052
BC64576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83724	WGS84	BC64576	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64576_Pcan_Bot2011_IGS	ADEAPC-48	1	BC64576_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64576	Sample	BC64576	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-48	IGS	45	BC64576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-48	CGTAATCCTCAGTACTGGCTA	138.51052
BC64576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83724	WGS84	BC64576	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.367	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64576_Sspa_Oph2007_COI	ADEAPC-48	1	BC64576_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64576	Sample	BC64576	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-48	COI	45	BC64576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-48	GAAGGCCTTCCGATGATC	138.51052
BC64576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83724	WGS84	BC64576	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64576_Upin_Bot2011_COI	ADEAPC-48	1	BC64576_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64576	Sample	BC64576	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-48	COI	45	BC64576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-48	CTTTAATTACAGCGTTTTTATTGTTGT	138.51052
BC64577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83723	WGS84	BC64577	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64577_Cjap_Sim2018_COI	ADEAPC-47	1	BC64577_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64577	Sample	BC64577	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-47	COI	45	BC64577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-47	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5104
BC64577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83723	WGS84	BC64577	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.281	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64577_Mgig_Bot2012_COI	ADEAPC-47	1	BC64577_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64577	Sample	BC64577	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-47	COI	45	BC64577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-47	TCTTATTCGTTGGAGACTTTATAACCCT	138.5104
BC64578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8079	WGS84	BC64578	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64578_Cmae_Bot2011_COI	ADEAPC-46	1	BC64578_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64578	Sample	BC64578	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-46	COI	45	BC64578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-46	ATGAACAGTCTATCCTCCTTTAG	138.54826
BC64580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80499	WGS84	BC64580	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64580_Cjap_Sim2018_COI	ADEAPC-44	1	BC64580_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64580	Sample	BC64580	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-44	COI	45	BC64580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-44	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54584
BC64580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80499	WGS84	BC64580	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.226	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64580_Mgig_Bot2012_COI	ADEAPC-44	1	BC64580_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64580	Sample	BC64580	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-44	COI	45	BC64580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-44	TCTTATTCGTTGGAGACTTTATAACCCT	138.54584
BC64581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80329	WGS84	BC64581	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64581_Cmae_Bot2011_COI	ADEAPC-43	1	BC64581_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64581	Sample	BC64581	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-43	COI	45	BC64581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-43	ATGAACAGTCTATCCTCCTTTAG	138.54832
BC64583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79895	WGS84	BC64583	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64583_Aamu_Bax2006_COI	ADEAPC-41	1	BC64583_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64583	Sample	BC64583	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-41	COI	45	BC64583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-41	GCACAACCGGGATCTTTACTTC	138.54817
BC64583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79895	WGS84	BC64583	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64583_Pcan_Bot2011_IGS	ADEAPC-41	1	BC64583_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64583	Sample	BC64583	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-41	IGS	45	BC64583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-41	CGTAATCCTCAGTACTGGCTA	138.54817
BC64583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79895	WGS84	BC64583	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64583_Sspa_Oph2007_COI	ADEAPC-41	1	BC64583_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64583	Sample	BC64583	targeted	Absent	Sample	Sspa_Oph2007_COI	ADEAPC-41	COI	45	BC64583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-41	GAAGGCCTTCCGATGATC	138.54817
BC64583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79895	WGS84	BC64583	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64583_Upin_Bot2011_COI	ADEAPC-41	1	BC64583_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64583	Sample	BC64583	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-41	COI	45	BC64583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-41	CTTTAATTACAGCGTTTTTATTGTTGT	138.54817
BC64584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79447	WGS84	BC64584	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64584_Cjap_Sim2018_COI	ADEAPC-40	1	BC64584_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64584	Sample	BC64584	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-40	COI	45	BC64584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-40	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54594
BC64584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79447	WGS84	BC64584	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.525	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64584_Mgig_Bot2012_COI	ADEAPC-40	1	BC64584_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64584	Sample	BC64584	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-40	COI	45	BC64584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-40	TCTTATTCGTTGGAGACTTTATAACCCT	138.54594
BC64585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79078	WGS84	BC64585	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64585_Cmae_Bot2011_COI	ADEAPC-39	1	BC64585_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64585	Sample	BC64585	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-39	COI	45	BC64585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-39	ATGAACAGTCTATCCTCCTTTAG	138.54425
BC64587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78384	WGS84	BC64587	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64587_Aamu_Bax2006_COI	ADEAPC-37	1	BC64587_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64587	Sample	BC64587	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-37	COI	45	BC64587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-37	GCACAACCGGGATCTTTACTTC	138.54032
BC64587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78384	WGS84	BC64587	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64587_Pcan_Bot2011_IGS	ADEAPC-37	1	BC64587_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64587	Sample	BC64587	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-37	IGS	45	BC64587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-37	CGTAATCCTCAGTACTGGCTA	138.54032
BC64587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78384	WGS84	BC64587	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.697	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64587_Sspa_Oph2007_COI	ADEAPC-37	1	BC64587_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64587	Sample	BC64587	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-37	COI	45	BC64587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-37	GAAGGCCTTCCGATGATC	138.54032
BC64587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78384	WGS84	BC64587	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64587_Upin_Bot2011_COI	ADEAPC-37	1	BC64587_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64587	Sample	BC64587	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-37	COI	45	BC64587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-37	CTTTAATTACAGCGTTTTTATTGTTGT	138.54032
BC64588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78076	WGS84	BC64588	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64588_Cjap_Sim2018_COI	ADEAPC-36	1	BC64588_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64588	Sample	BC64588	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-36	COI	45	BC64588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-36	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5374
BC64588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78076	WGS84	BC64588	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.549	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64588_Mgig_Bot2012_COI	ADEAPC-36	1	BC64588_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64588	Sample	BC64588	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-36	COI	45	BC64588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-36	TCTTATTCGTTGGAGACTTTATAACCCT	138.5374
BC64589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77762	WGS84	BC64589	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64589_Cmae_Bot2011_COI	ADEAPC-35	1	BC64589_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64589	Sample	BC64589	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-35	COI	45	BC64589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-35	ATGAACAGTCTATCCTCCTTTAG	138.53481
BC64590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77469	WGS84	BC64590	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64590_Aamu_Bax2006_COI	ADEAPC-34	1	BC64590_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64590	Sample	BC64590	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-34	COI	45	BC64590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-34	GCACAACCGGGATCTTTACTTC	138.53169
BC64590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77469	WGS84	BC64590	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64590_Pcan_Bot2011_IGS	ADEAPC-34	1	BC64590_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64590	Sample	BC64590	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-34	IGS	45	BC64590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-34	CGTAATCCTCAGTACTGGCTA	138.53169
BC64590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77469	WGS84	BC64590	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64590_Sspa_Oph2007_COI	ADEAPC-34	1	BC64590_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64590	Sample	BC64590	targeted	Absent	Sample	Sspa_Oph2007_COI	ADEAPC-34	COI	45	BC64590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-34	GAAGGCCTTCCGATGATC	138.53169
BC64590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77469	WGS84	BC64590	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64590_Upin_Bot2011_COI	ADEAPC-34	1	BC64590_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64590	Sample	BC64590	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-34	COI	45	BC64590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-34	CTTTAATTACAGCGTTTTTATTGTTGT	138.53169
BC64591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77128	WGS84	BC64591	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64591_Cjap_Sim2018_COI	ADEAPC-33	1	BC64591_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64591	Sample	BC64591	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-33	COI	45	BC64591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-33	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52947
BC64591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77128	WGS84	BC64591	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.64	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64591_Mgig_Bot2012_COI	ADEAPC-33	1	BC64591_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64591	Sample	BC64591	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-33	COI	45	BC64591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-33	TCTTATTCGTTGGAGACTTTATAACCCT	138.52947
BC64592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76757	WGS84	BC64592	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64592_Cmae_Bot2011_COI	ADEAPC-32	1	BC64592_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64592	Sample	BC64592	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-32	COI	45	BC64592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-32	ATGAACAGTCTATCCTCCTTTAG	138.5283
BC64594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76096	WGS84	BC64594	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64594_Aamu_Bax2006_COI	ADEAPC-30	1	BC64594_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64594	Sample	BC64594	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-30	COI	45	BC64594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-30	GCACAACCGGGATCTTTACTTC	138.52349
BC64594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76096	WGS84	BC64594	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64594_Pcan_Bot2011_IGS	ADEAPC-30	1	BC64594_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64594	Sample	BC64594	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-30	IGS	45	BC64594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-30	CGTAATCCTCAGTACTGGCTA	138.52349
BC64594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76096	WGS84	BC64594	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64594_Sspa_Oph2007_COI	ADEAPC-30	1	BC64594_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64594	Sample	BC64594	targeted	Absent	Sample	Sspa_Oph2007_COI	ADEAPC-30	COI	45	BC64594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-30	GAAGGCCTTCCGATGATC	138.52349
BC64594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76096	WGS84	BC64594	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64594_Upin_Bot2011_COI	ADEAPC-30	1	BC64594_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64594	Sample	BC64594	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-30	COI	45	BC64594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-30	CTTTAATTACAGCGTTTTTATTGTTGT	138.52349
BC64595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.75796	WGS84	BC64595	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64595_Cjap_Sim2018_COI	ADEAPC-29	1	BC64595_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64595	Sample	BC64595	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-29	COI	45	BC64595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-29	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52051
BC64595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.75796	WGS84	BC64595	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.107	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64595_Mgig_Bot2012_COI	ADEAPC-29	1	BC64595_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64595	Sample	BC64595	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-29	COI	45	BC64595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-29	TCTTATTCGTTGGAGACTTTATAACCCT	138.52051
BC64596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81098	WGS84	BC64596	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64596_Cmae_Bot2011_COI	ADEAPC-28	1	BC64596_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64596	Sample	BC64596	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-28	COI	45	BC64596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-28	ATGAACAGTCTATCCTCCTTTAG	138.52321
BC64598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80908	WGS84	BC64598	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64598_Aamu_Bax2006_COI	ADEAPC-26	1	BC64598_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64598	Sample	BC64598	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-26	COI	45	BC64598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-26	GCACAACCGGGATCTTTACTTC	138.51913
BC64598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80908	WGS84	BC64598	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64598_Pcan_Bot2011_IGS	ADEAPC-26	1	BC64598_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64598	Sample	BC64598	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-26	IGS	45	BC64598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-26	CGTAATCCTCAGTACTGGCTA	138.51913
BC64598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80908	WGS84	BC64598	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.476	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64598_Sspa_Oph2007_COI	ADEAPC-26	1	BC64598_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64598	Sample	BC64598	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-26	COI	45	BC64598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-26	GAAGGCCTTCCGATGATC	138.51913
BC64598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80908	WGS84	BC64598	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64598_Upin_Bot2011_COI	ADEAPC-26	1	BC64598_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64598	Sample	BC64598	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-26	COI	45	BC64598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-26	CTTTAATTACAGCGTTTTTATTGTTGT	138.51913
BC64599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80869	WGS84	BC64599	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64599_Cjap_Sim2018_COI	ADEAPC-25	1	BC64599_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64599	Sample	BC64599	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-25	COI	45	BC64599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-25	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5177
BC64599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80869	WGS84	BC64599	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.913	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64599_Mgig_Bot2012_COI	ADEAPC-25	1	BC64599_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64599	Sample	BC64599	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-25	COI	45	BC64599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-25	TCTTATTCGTTGGAGACTTTATAACCCT	138.5177
BC64600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80373	WGS84	BC64600	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64600_Cmae_Bot2011_COI	ADEAPC-24	1	BC64600_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64600	Sample	BC64600	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-24	COI	45	BC64600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-24	ATGAACAGTCTATCCTCCTTTAG	138.51423
BC64602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79792	WGS84	BC64602	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64602_Aamu_Bax2006_COI	ADEAPC-22	1	BC64602_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64602	Sample	BC64602	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-22	COI	45	BC64602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-22	GCACAACCGGGATCTTTACTTC	138.51337
BC64602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79792	WGS84	BC64602	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64602_Pcan_Bot2011_IGS	ADEAPC-22	1	BC64602_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64602	Sample	BC64602	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-22	IGS	45	BC64602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-22	CGTAATCCTCAGTACTGGCTA	138.51337
BC64602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79792	WGS84	BC64602	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.695	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64602_Sspa_Oph2007_COI	ADEAPC-22	1	BC64602_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64602	Sample	BC64602	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-22	COI	45	BC64602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-22	GAAGGCCTTCCGATGATC	138.51337
BC64602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79792	WGS84	BC64602	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64602_Upin_Bot2011_COI	ADEAPC-22	1	BC64602_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64602	Sample	BC64602	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-22	COI	45	BC64602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-22	CTTTAATTACAGCGTTTTTATTGTTGT	138.51337
BC64603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79528	WGS84	BC64603	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64603_Cjap_Sim2018_COI	ADEAPC-21	1	BC64603_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64603	Sample	BC64603	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-21	COI	45	BC64603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-21	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51364
BC64603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79528	WGS84	BC64603	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.347	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64603_Mgig_Bot2012_COI	ADEAPC-21	1	BC64603_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64603	Sample	BC64603	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-21	COI	45	BC64603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-21	TCTTATTCGTTGGAGACTTTATAACCCT	138.51364
BC64604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79054	WGS84	BC64604	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64604_Cmae_Bot2011_COI	ADEAPC-20	1	BC64604_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64604	Sample	BC64604	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-20	COI	45	BC64604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-20	ATGAACAGTCTATCCTCCTTTAG	138.51486
BC64606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78608	WGS84	BC64606	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64606_Aamu_Bax2006_COI	ADEAPC-18	1	BC64606_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64606	Sample	BC64606	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-18	COI	45	BC64606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-18	GCACAACCGGGATCTTTACTTC	138.51602
BC64606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78608	WGS84	BC64606	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64606_Pcan_Bot2011_IGS	ADEAPC-18	1	BC64606_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64606	Sample	BC64606	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-18	IGS	45	BC64606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-18	CGTAATCCTCAGTACTGGCTA	138.51602
BC64606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78608	WGS84	BC64606	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.135	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64606_Sspa_Oph2007_COI	ADEAPC-18	1	BC64606_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64606	Sample	BC64606	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-18	COI	45	BC64606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-18	GAAGGCCTTCCGATGATC	138.51602
BC64606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78608	WGS84	BC64606	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64606_Upin_Bot2011_COI	ADEAPC-18	1	BC64606_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64606	Sample	BC64606	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-18	COI	45	BC64606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.51602
BC64607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78364	WGS84	BC64607	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64607_Cjap_Sim2018_COI	ADEAPC-17	1	BC64607_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64607	Sample	BC64607	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-17	COI	45	BC64607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51668
BC64607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78364	WGS84	BC64607	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.621	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64607_Mgig_Bot2012_COI	ADEAPC-17	1	BC64607_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64607	Sample	BC64607	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-17	COI	45	BC64607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.51668
BC64608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78074	WGS84	BC64608	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64608_Cmae_Bot2011_COI	ADEAPC-16	1	BC64608_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64608	Sample	BC64608	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-16	COI	45	BC64608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-16	ATGAACAGTCTATCCTCCTTTAG	138.51766
BC64610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77268	WGS84	BC64610	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64610_Cjap_Sim2018_COI	ADEAPC-14	1	BC64610_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64610	Sample	BC64610	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-14	COI	45	BC64610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51969
BC64610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77268	WGS84	BC64610	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.115	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64610_Mgig_Bot2012_COI	ADEAPC-14	1	BC64610_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64610	Sample	BC64610	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-14	COI	45	BC64610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.51969
BC64611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76972	WGS84	BC64611	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64611_Cmae_Bot2011_COI	ADEAPC-13	1	BC64611_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64611	Sample	BC64611	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-13	COI	45	BC64611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-13	ATGAACAGTCTATCCTCCTTTAG	138.51927
BC64613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76148	WGS84	BC64613	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64613_Aamu_Bax2006_COI	ADEAPC-11	1	BC64613_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64613	Sample	BC64613	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-11	COI	45	BC64613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-11	GCACAACCGGGATCTTTACTTC	138.51153
BC64613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76148	WGS84	BC64613	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64613_Pcan_Bot2011_IGS	ADEAPC-11	1	BC64613_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64613	Sample	BC64613	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-11	IGS	45	BC64613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-11	CGTAATCCTCAGTACTGGCTA	138.51153
BC64613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76148	WGS84	BC64613	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.136	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64613_Sspa_Oph2007_COI	ADEAPC-11	1	BC64613_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64613	Sample	BC64613	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-11	COI	45	BC64613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-11	GAAGGCCTTCCGATGATC	138.51153
BC64613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76148	WGS84	BC64613	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64613_Upin_Bot2011_COI	ADEAPC-11	1	BC64613_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64613	Sample	BC64613	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-11	COI	45	BC64613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.51153
BC64614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76032	WGS84	BC64614	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64614_Cjap_Sim2018_COI	ADEAPC-10	1	BC64614_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64614	Sample	BC64614	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-10	COI	45	BC64614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50655
BC64614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76032	WGS84	BC64614	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.014	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64614_Mgig_Bot2012_COI	ADEAPC-10	1	BC64614_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64614	Sample	BC64614	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-10	COI	45	BC64614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.50655
BC64615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76007	WGS84	BC64615	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.785	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64615_Cmae_Bot2011_COI	ADEAPC-09	1	BC64615_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64615	Sample	BC64615	targeted	Present	Sample	Cmae_Bot2011_COI	ADEAPC-09	COI	45	BC64615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-09	ATGAACAGTCTATCCTCCTTTAG	138.50254
BC64617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76304	WGS84	BC64617	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64617_Aamu_Bax2006_COI	ADEAPC-07	1	BC64617_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64617	Sample	BC64617	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-07	COI	45	BC64617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-07	GCACAACCGGGATCTTTACTTC	138.49257
BC64617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76304	WGS84	BC64617	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64617_Pcan_Bot2011_IGS	ADEAPC-07	1	BC64617_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64617	Sample	BC64617	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-07	IGS	45	BC64617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-07	CGTAATCCTCAGTACTGGCTA	138.49257
BC64617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76304	WGS84	BC64617	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.503	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64617_Sspa_Oph2007_COI	ADEAPC-07	1	BC64617_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64617	Sample	BC64617	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-07	COI	45	BC64617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-07	GAAGGCCTTCCGATGATC	138.49257
BC64617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76304	WGS84	BC64617	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64617_Upin_Bot2011_COI	ADEAPC-07	1	BC64617_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64617	Sample	BC64617	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-07	COI	45	BC64617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.49257
BC64618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76372	WGS84	BC64618	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64618_Cjap_Sim2018_COI	ADEAPC-06	1	BC64618_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64618	Sample	BC64618	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-06	COI	45	BC64618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49151
BC64618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76372	WGS84	BC64618	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.415	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64618_Mgig_Bot2012_COI	ADEAPC-06	1	BC64618_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64618	Sample	BC64618	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-06	COI	45	BC64618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.49151
BC64619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76454	WGS84	BC64619	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.486	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64619_Cmae_Bot2011_COI	ADEAPC-05	1	BC64619_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64619	Sample	BC64619	targeted	Present	Sample	Cmae_Bot2011_COI	ADEAPC-05	COI	45	BC64619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-05	ATGAACAGTCTATCCTCCTTTAG	138.49035
BC64620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76871	WGS84	BC64620	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64620_Aamu_Bax2006_COI	ADEAPC-04	1	BC64620_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64620	Sample	BC64620	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-04	COI	45	BC64620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-04	GCACAACCGGGATCTTTACTTC	138.48329
BC64620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76871	WGS84	BC64620	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64620_Pcan_Bot2011_IGS	ADEAPC-04	1	BC64620_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64620	Sample	BC64620	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-04	IGS	45	BC64620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-04	CGTAATCCTCAGTACTGGCTA	138.48329
BC64620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76871	WGS84	BC64620	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.686	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64620_Sspa_Oph2007_COI	ADEAPC-04	1	BC64620_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64620	Sample	BC64620	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-04	COI	45	BC64620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-04	GAAGGCCTTCCGATGATC	138.48329
BC64620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76871	WGS84	BC64620	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64620_Upin_Bot2011_COI	ADEAPC-04	1	BC64620_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64620	Sample	BC64620	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-04	COI	45	BC64620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.48329
BC64621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76978	WGS84	BC64621	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64621_Cjap_Sim2018_COI	ADEAPC-03	1	BC64621_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64621	Sample	BC64621	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-03	COI	45	BC64621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48298
BC64621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76978	WGS84	BC64621	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.672	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64621_Mgig_Bot2012_COI	ADEAPC-03	1	BC64621_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64621	Sample	BC64621	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-03	COI	45	BC64621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.48298
BC64622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.7704	WGS84	BC64622	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.793	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64622_Cmae_Bot2011_COI	ADEAPC-02	1	BC64622_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64622	Sample	BC64622	targeted	Present	Sample	Cmae_Bot2011_COI	ADEAPC-02	COI	45	BC64622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-02	ATGAACAGTCTATCCTCCTTTAG	138.48253
BC77251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84457	WGS84	BC77251	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77251_Aamu_Bax2006_COI	S5-04	1	BC77251_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77251	Sample	BC77251	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-04	COI	45	BC77251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-04	GCACAACCGGGATCTTTACTTC	138.49741
BC77251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84457	WGS84	BC77251	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77251_Pcan_Bot2011_IGS	S5-04	1	BC77251_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77251	Sample	BC77251	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-04	IGS	45	BC77251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-04	CGTAATCCTCAGTACTGGCTA	138.49741
BC77251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84457	WGS84	BC77251	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.009	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77251_Sspa_Oph2007_COI	S5-04	1	BC77251_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77251	Sample	BC77251	targeted	Present	Sample	Sspa_Oph2007_COI	S5-04	COI	45	BC77251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-04	GAAGGCCTTCCGATGATC	138.49741
BC77251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84457	WGS84	BC77251	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77251_Upin_Bot2011_COI	S5-04	1	BC77251_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77251	Sample	BC77251	targeted	Absent	Sample	Upin_Bot2011_COI	S5-04	COI	45	BC77251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.49741
BC77252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83697	WGS84	BC77252	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77252_Cjap_Sim2018_COI	S5-09	1	BC77252_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77252	Sample	BC77252	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-09	COI	45	BC77252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51049
BC77252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83697	WGS84	BC77252	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.493	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77252_Mgig_Bot2012_COI	S5-09	1	BC77252_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77252	Sample	BC77252	targeted	Present	Sample	Mgig_Bot2012_COI	S5-09	COI	45	BC77252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51049
BC77253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78389	WGS84	BC77253	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77253_Cmae_Bot2011_COI	S5-14	1	BC77253_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77253	Sample	BC77253	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-14	COI	45	BC77253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-14	ATGAACAGTCTATCCTCCTTTAG	138.51561
BC77255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81058	WGS84	BC77255	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77255_Aamu_Bax2006_COI	S5-23	1	BC77255_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77255	Sample	BC77255	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-23	COI	45	BC77255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-23	GCACAACCGGGATCTTTACTTC	138.52126
BC77255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81058	WGS84	BC77255	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77255_Pcan_Bot2011_IGS	S5-23	1	BC77255_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77255	Sample	BC77255	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-23	IGS	45	BC77255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-23	CGTAATCCTCAGTACTGGCTA	138.52126
BC77255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81058	WGS84	BC77255	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.9	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77255_Sspa_Oph2007_COI	S5-23	1	BC77255_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77255	Sample	BC77255	targeted	Present	Sample	Sspa_Oph2007_COI	S5-23	COI	45	BC77255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-23	GAAGGCCTTCCGATGATC	138.52126
BC77255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81058	WGS84	BC77255	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77255_Upin_Bot2011_COI	S5-23	1	BC77255_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77255	Sample	BC77255	targeted	Absent	Sample	Upin_Bot2011_COI	S5-23	COI	45	BC77255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-23	CTTTAATTACAGCGTTTTTATTGTTGT	138.52126
BC77256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84392	WGS84	BC77256	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77256_Cjap_Sim2018_COI	S6-04	1	BC77256_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77256	Sample	BC77256	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-04	COI	45	BC77256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49698
BC77256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84392	WGS84	BC77256	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77256_Mgig_Bot2012_COI	S6-04	1	BC77256_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77256	Sample	BC77256	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-04	COI	45	BC77256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49698
BC77257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83669	WGS84	BC77257	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77257_Cmae_Bot2011_COI	S6-09	1	BC77257_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77257	Sample	BC77257	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-09	COI	45	BC77257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-09	ATGAACAGTCTATCCTCCTTTAG	138.5098
BC77259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77244	WGS84	BC77259	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77259_Aamu_Bax2006_COI	S6-19	1	BC77259_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77259	Sample	BC77259	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-19	COI	45	BC77259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-19	GCACAACCGGGATCTTTACTTC	138.48557
BC77259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77244	WGS84	BC77259	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77259_Pcan_Bot2011_IGS	S6-19	1	BC77259_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77259	Sample	BC77259	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-19	IGS	45	BC77259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-19	CGTAATCCTCAGTACTGGCTA	138.48557
BC77259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77244	WGS84	BC77259	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.884	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77259_Sspa_Oph2007_COI	S6-19	1	BC77259_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77259	Sample	BC77259	targeted	Present	Sample	Sspa_Oph2007_COI	S6-19	COI	45	BC77259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-19	GAAGGCCTTCCGATGATC	138.48557
BC77259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77244	WGS84	BC77259	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77259_Upin_Bot2011_COI	S6-19	1	BC77259_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77259	Sample	BC77259	targeted	Absent	Sample	Upin_Bot2011_COI	S6-19	COI	45	BC77259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.48557
BC77260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81082	WGS84	BC77260	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77260_Cmae_Bot2011_COI	S6-23	1	BC77260_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77260	Sample	BC77260	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-23	COI	45	BC77260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-23	ATGAACAGTCTATCCTCCTTTAG	138.52258
BC77262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83653	WGS84	BC77262	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77262_Aamu_Bax2006_COI	S7-09	1	BC77262_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77262	Sample	BC77262	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-09	COI	45	BC77262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-09	GCACAACCGGGATCTTTACTTC	138.50914
BC77262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83653	WGS84	BC77262	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77262_Pcan_Bot2011_IGS	S7-09	1	BC77262_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77262	Sample	BC77262	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-09	IGS	45	BC77262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-09	CGTAATCCTCAGTACTGGCTA	138.50914
BC77262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83653	WGS84	BC77262	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77262_Sspa_Oph2007_COI	S7-09	1	BC77262_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77262	Sample	BC77262	targeted	Absent	Sample	Sspa_Oph2007_COI	S7-09	COI	45	BC77262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-09	GAAGGCCTTCCGATGATC	138.50914
BC77262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83653	WGS84	BC77262	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77262_Upin_Bot2011_COI	S7-09	1	BC77262_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77262	Sample	BC77262	targeted	Absent	Sample	Upin_Bot2011_COI	S7-09	COI	45	BC77262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.50914
BC77263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78246	WGS84	BC77263	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77263_Cjap_Sim2018_COI	S7-14	1	BC77263_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77263	Sample	BC77263	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-14	COI	45	BC77263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51648
BC77263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78246	WGS84	BC77263	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77263_Mgig_Bot2012_COI	S7-14	1	BC77263_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77263	Sample	BC77263	targeted	Present	Sample	Mgig_Bot2012_COI	S7-14	COI	45	BC77263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.51648
BC77264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77342	WGS84	BC77264	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77264_Cmae_Bot2011_COI	S7-19	1	BC77264_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77264	Sample	BC77264	targeted	Absent	Sample	Cmae_Bot2011_COI	S7-19	COI	45	BC77264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-19	ATGAACAGTCTATCCTCCTTTAG	138.48496
BC77266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81019	WGS84	BC77266	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77266_Aamu_Bax2006_COI	S8-04	1	BC77266_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77266	Sample	BC77266	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-04	COI	45	BC77266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-04	GCACAACCGGGATCTTTACTTC	138.52036
BC77266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81019	WGS84	BC77266	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77266_Pcan_Bot2011_IGS	S8-04	1	BC77266_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77266	Sample	BC77266	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-04	IGS	45	BC77266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-04	CGTAATCCTCAGTACTGGCTA	138.52036
BC77266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81019	WGS84	BC77266	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.416	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77266_Sspa_Oph2007_COI	S8-04	1	BC77266_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77266	Sample	BC77266	targeted	Present	Sample	Sspa_Oph2007_COI	S8-04	COI	45	BC77266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-04	GAAGGCCTTCCGATGATC	138.52036
BC77266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81019	WGS84	BC77266	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77266_Upin_Bot2011_COI	S8-04	1	BC77266_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77266	Sample	BC77266	targeted	Absent	Sample	Upin_Bot2011_COI	S8-04	COI	45	BC77266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.52036
BC77267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78552	WGS84	BC77267	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77267_Cjap_Sim2018_COI	S8-09	1	BC77267_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77267	Sample	BC77267	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-09	COI	45	BC77267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51387
BC77267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78552	WGS84	BC77267	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77267_Mgig_Bot2012_COI	S8-09	1	BC77267_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77267	Sample	BC77267	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-09	COI	45	BC77267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51387
BC77268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7737	WGS84	BC77268	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77268_Cmae_Bot2011_COI	S8-14	1	BC77268_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77268	Sample	BC77268	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-14	COI	45	BC77268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-14	ATGAACAGTCTATCCTCCTTTAG	138.48409
BC77270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83692	WGS84	BC77270	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77270_Cjap_Sim2018_COI	S8-23	1	BC77270_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77270	Sample	BC77270	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-23	COI	45	BC77270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-23	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50975
BC77270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83692	WGS84	BC77270	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77270_Mgig_Bot2012_COI	S8-23	1	BC77270_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77270	Sample	BC77270	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-23	COI	45	BC77270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-23	TCTTATTCGTTGGAGACTTTATAACCCT	138.50975
BC77271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77271	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77271_Cmae_Bot2011_COI	S16-10	1	BC77271_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77271	Sample	BC77271	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-10	COI	45	BC77271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-10	ATGAACAGTCTATCCTCCTTTAG	138.52039
BC77273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83638	WGS84	BC77273	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77273_Aamu_Bax2006_COI	S16-03	1	BC77273_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77273	Sample	BC77273	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-03	COI	45	BC77273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-03	GCACAACCGGGATCTTTACTTC	138.50866
BC77273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83638	WGS84	BC77273	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77273_Pcan_Bot2011_IGS	S16-03	1	BC77273_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77273	Sample	BC77273	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-03	IGS	45	BC77273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-03	CGTAATCCTCAGTACTGGCTA	138.50866
BC77273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83638	WGS84	BC77273	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.924	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77273_Sspa_Oph2007_COI	S16-03	1	BC77273_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77273	Sample	BC77273	targeted	Present	Sample	Sspa_Oph2007_COI	S16-03	COI	45	BC77273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-03	GAAGGCCTTCCGATGATC	138.50866
BC77273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83638	WGS84	BC77273	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77273_Upin_Bot2011_COI	S16-03	1	BC77273_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77273	Sample	BC77273	targeted	Absent	Sample	Upin_Bot2011_COI	S16-03	COI	45	BC77273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.50866
BC77274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83522	WGS84	BC77274	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77274_Cjap_Sim2018_COI	S16-04	1	BC77274_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77274	Sample	BC77274	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-04	COI	45	BC77274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50734
BC77274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83522	WGS84	BC77274	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.571	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77274_Mgig_Bot2012_COI	S16-04	1	BC77274_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77274	Sample	BC77274	targeted	Present	Sample	Mgig_Bot2012_COI	S16-04	COI	45	BC77274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.50734
BC77275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83426	WGS84	BC77275	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77275_Cmae_Bot2011_COI	S16-05	1	BC77275_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77275	Sample	BC77275	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-05	COI	45	BC77275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-05	ATGAACAGTCTATCCTCCTTTAG	138.50758
BC77277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77277	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77277_Aamu_Bax2006_COI	S16-07	1	BC77277_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77277	Sample	BC77277	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-07	COI	45	BC77277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-07	GCACAACCGGGATCTTTACTTC	138.51754
BC77277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77277	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77277_Pcan_Bot2011_IGS	S16-07	1	BC77277_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77277	Sample	BC77277	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-07	IGS	45	BC77277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-07	CGTAATCCTCAGTACTGGCTA	138.51754
BC77277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77277	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77277_Sspa_Oph2007_COI	S16-07	1	BC77277_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77277	Sample	BC77277	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-07	COI	45	BC77277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-07	GAAGGCCTTCCGATGATC	138.51754
BC77277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77277	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77277_Upin_Bot2011_COI	S16-07	1	BC77277_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77277	Sample	BC77277	targeted	Absent	Sample	Upin_Bot2011_COI	S16-07	COI	45	BC77277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.51754
BC77278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77278	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77278_Cjap_Sim2018_COI	S16-08	1	BC77278_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77278	Sample	BC77278	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-08	COI	45	BC77278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51838
BC77278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77278	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.968	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77278_Mgig_Bot2012_COI	S16-08	1	BC77278_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77278	Sample	BC77278	targeted	Present	Sample	Mgig_Bot2012_COI	S16-08	COI	45	BC77278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51838
BC77279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80992	WGS84	BC77279	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77279_Cmae_Bot2011_COI	S16-09	1	BC77279_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77279	Sample	BC77279	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-09	COI	45	BC77279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-09	ATGAACAGTCTATCCTCCTTTAG	138.51928
BC77280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83901	WGS84	BC77280	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77280_Aamu_Bax2006_COI	S16-01	1	BC77280_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77280	Sample	BC77280	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-01	COI	45	BC77280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-01	GCACAACCGGGATCTTTACTTC	138.50604
BC77280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83901	WGS84	BC77280	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77280_Pcan_Bot2011_IGS	S16-01	1	BC77280_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77280	Sample	BC77280	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-01	IGS	45	BC77280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-01	CGTAATCCTCAGTACTGGCTA	138.50604
BC77280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83901	WGS84	BC77280	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77280_Sspa_Oph2007_COI	S16-01	1	BC77280_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77280	Sample	BC77280	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-01	COI	45	BC77280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-01	GAAGGCCTTCCGATGATC	138.50604
BC77280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83901	WGS84	BC77280	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77280_Upin_Bot2011_COI	S16-01	1	BC77280_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77280	Sample	BC77280	targeted	Absent	Sample	Upin_Bot2011_COI	S16-01	COI	45	BC77280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.50604
BC77281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77315	WGS84	BC77281	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77281_Cjap_Sim2018_COI	S17-01	1	BC77281_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77281	Sample	BC77281	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-01	COI	45	BC77281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48546
BC77281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77315	WGS84	BC77281	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.39	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77281_Mgig_Bot2012_COI	S17-01	1	BC77281_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77281	Sample	BC77281	targeted	Present	Sample	Mgig_Bot2012_COI	S17-01	COI	45	BC77281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.48546
BC77282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77369	WGS84	BC77282	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77282_Cmae_Bot2011_COI	S17-02	1	BC77282_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77282	Sample	BC77282	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-02	COI	45	BC77282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-02	ATGAACAGTCTATCCTCCTTTAG	138.48594
BC77284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77691	WGS84	BC77284	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77284_Aamu_Bax2006_COI	S17-04	1	BC77284_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77284	Sample	BC77284	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-04	COI	45	BC77284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-04	GCACAACCGGGATCTTTACTTC	138.51832
BC77284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77691	WGS84	BC77284	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77284_Pcan_Bot2011_IGS	S17-04	1	BC77284_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77284	Sample	BC77284	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-04	IGS	45	BC77284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-04	CGTAATCCTCAGTACTGGCTA	138.51832
BC77284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77691	WGS84	BC77284	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77284_Sspa_Oph2007_COI	S17-04	1	BC77284_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77284	Sample	BC77284	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-04	COI	45	BC77284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-04	GAAGGCCTTCCGATGATC	138.51832
BC77284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77691	WGS84	BC77284	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77284_Upin_Bot2011_COI	S17-04	1	BC77284_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77284	Sample	BC77284	targeted	Absent	Sample	Upin_Bot2011_COI	S17-04	COI	45	BC77284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.51832
BC77285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77779	WGS84	BC77285	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77285_Cjap_Sim2018_COI	S17-05	1	BC77285_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77285	Sample	BC77285	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-05	COI	45	BC77285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51794
BC77285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77779	WGS84	BC77285	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.985	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77285_Mgig_Bot2012_COI	S17-05	1	BC77285_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77285	Sample	BC77285	targeted	Present	Sample	Mgig_Bot2012_COI	S17-05	COI	45	BC77285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.51794
BC77286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77881	WGS84	BC77286	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77286_Cmae_Bot2011_COI	S17-06	1	BC77286_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77286	Sample	BC77286	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-06	COI	45	BC77286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-06	ATGAACAGTCTATCCTCCTTTAG	138.51774
BC77288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80998	WGS84	BC77288	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77288_Aamu_Bax2006_COI	S17-08	1	BC77288_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77288	Sample	BC77288	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-08	COI	45	BC77288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-08	GCACAACCGGGATCTTTACTTC	138.51955
BC77288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80998	WGS84	BC77288	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77288_Pcan_Bot2011_IGS	S17-08	1	BC77288_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77288	Sample	BC77288	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-08	IGS	45	BC77288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-08	CGTAATCCTCAGTACTGGCTA	138.51955
BC77288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80998	WGS84	BC77288	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.906	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77288_Sspa_Oph2007_COI	S17-08	1	BC77288_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77288	Sample	BC77288	targeted	Present	Sample	Sspa_Oph2007_COI	S17-08	COI	45	BC77288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-08	GAAGGCCTTCCGATGATC	138.51955
BC77288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80998	WGS84	BC77288	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77288_Upin_Bot2011_COI	S17-08	1	BC77288_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77288	Sample	BC77288	targeted	Absent	Sample	Upin_Bot2011_COI	S17-08	COI	45	BC77288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51955
BC77289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81059	WGS84	BC77289	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77289_Cjap_Sim2018_COI	S17-09	1	BC77289_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77289	Sample	BC77289	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-09	COI	45	BC77289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52046
BC77289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81059	WGS84	BC77289	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.474	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77289_Mgig_Bot2012_COI	S17-09	1	BC77289_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77289	Sample	BC77289	targeted	Present	Sample	Mgig_Bot2012_COI	S17-09	COI	45	BC77289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.52046
BC77291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.844	WGS84	BC77291	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77291_Aamu_Bax2006_COI	S17-11	1	BC77291_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77291	Sample	BC77291	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-11	COI	45	BC77291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-11	GCACAACCGGGATCTTTACTTC	138.4977
BC77291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.844	WGS84	BC77291	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77291_Pcan_Bot2011_IGS	S17-11	1	BC77291_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77291	Sample	BC77291	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-11	IGS	45	BC77291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-11	CGTAATCCTCAGTACTGGCTA	138.4977
BC77291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.844	WGS84	BC77291	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77291_Sspa_Oph2007_COI	S17-11	1	BC77291_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77291	Sample	BC77291	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-11	COI	45	BC77291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-11	GAAGGCCTTCCGATGATC	138.4977
BC77291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.844	WGS84	BC77291	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77291_Upin_Bot2011_COI	S17-11	1	BC77291_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77291	Sample	BC77291	targeted	Absent	Sample	Upin_Bot2011_COI	S17-11	COI	45	BC77291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.4977
BC77292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84305	WGS84	BC77292	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77292_Cjap_Sim2018_COI	S17-12	1	BC77292_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77292	Sample	BC77292	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-12	COI	45	BC77292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49884
BC77292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84305	WGS84	BC77292	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.606	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77292_Mgig_Bot2012_COI	S17-12	1	BC77292_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77292	Sample	BC77292	targeted	Present	Sample	Mgig_Bot2012_COI	S17-12	COI	45	BC77292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.49884
BC77293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8367	WGS84	BC77293	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77293_Cmae_Bot2011_COI	S17-13	1	BC77293_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77293	Sample	BC77293	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-13	COI	45	BC77293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-13	ATGAACAGTCTATCCTCCTTTAG	138.50883
BC77295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83581	WGS84	BC77295	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77295_Aamu_Bax2006_COI	S17-15	1	BC77295_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77295	Sample	BC77295	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-15	COI	45	BC77295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-15	GCACAACCGGGATCTTTACTTC	138.50807
BC77295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83581	WGS84	BC77295	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77295_Pcan_Bot2011_IGS	S17-15	1	BC77295_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77295	Sample	BC77295	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-15	IGS	45	BC77295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-15	CGTAATCCTCAGTACTGGCTA	138.50807
BC77295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83581	WGS84	BC77295	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.806	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77295_Sspa_Oph2007_COI	S17-15	1	BC77295_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77295	Sample	BC77295	targeted	Present	Sample	Sspa_Oph2007_COI	S17-15	COI	45	BC77295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-15	GAAGGCCTTCCGATGATC	138.50807
BC77295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83581	WGS84	BC77295	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77295_Upin_Bot2011_COI	S17-15	1	BC77295_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77295	Sample	BC77295	targeted	Absent	Sample	Upin_Bot2011_COI	S17-15	COI	45	BC77295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-15	CTTTAATTACAGCGTTTTTATTGTTGT	138.50807
BC77296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83556	WGS84	BC77296	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77296_Cjap_Sim2018_COI	S18-01	1	BC77296_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77296	Sample	BC77296	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-01	COI	45	BC77296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50784
BC77296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83556	WGS84	BC77296	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77296_Mgig_Bot2012_COI	S18-01	1	BC77296_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77296	Sample	BC77296	targeted	Absent	Sample	Mgig_Bot2012_COI	S18-01	COI	45	BC77296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.50784
BC77297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83622	WGS84	BC77297	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77297_Cmae_Bot2011_COI	S18-02	1	BC77297_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77297	Sample	BC77297	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-02	COI	45	BC77297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-02	ATGAACAGTCTATCCTCCTTTAG	138.50888
BC77299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83696	WGS84	BC77299	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77299_Aamu_Bax2006_COI	S18-04	1	BC77299_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77299	Sample	BC77299	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-04	COI	45	BC77299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-04	GCACAACCGGGATCTTTACTTC	138.51075
BC77299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83696	WGS84	BC77299	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77299_Pcan_Bot2011_IGS	S18-04	1	BC77299_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77299	Sample	BC77299	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-04	IGS	45	BC77299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-04	CGTAATCCTCAGTACTGGCTA	138.51075
BC77299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83696	WGS84	BC77299	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.086	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77299_Sspa_Oph2007_COI	S18-04	1	BC77299_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77299	Sample	BC77299	targeted	Present	Sample	Sspa_Oph2007_COI	S18-04	COI	45	BC77299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-04	GAAGGCCTTCCGATGATC	138.51075
BC77299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83696	WGS84	BC77299	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77299_Upin_Bot2011_COI	S18-04	1	BC77299_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77299	Sample	BC77299	targeted	Absent	Sample	Upin_Bot2011_COI	S18-04	COI	45	BC77299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.51075
BC77300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83734	WGS84	BC77300	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77300_Cjap_Sim2018_COI	S18-05	1	BC77300_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77300	Sample	BC77300	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-05	COI	45	BC77300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51042
BC77300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83734	WGS84	BC77300	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.99	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77300_Mgig_Bot2012_COI	S18-05	1	BC77300_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77300	Sample	BC77300	targeted	Present	Sample	Mgig_Bot2012_COI	S18-05	COI	45	BC77300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.51042
BC77301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80836	WGS84	BC77301	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77301_Cmae_Bot2011_COI	S18-06	1	BC77301_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77301	Sample	BC77301	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-06	COI	45	BC77301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-06	ATGAACAGTCTATCCTCCTTTAG	138.51628
BC77303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77303	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77303_Aamu_Bax2006_COI	S18-08	1	BC77303_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77303	Sample	BC77303	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-08	COI	45	BC77303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-08	GCACAACCGGGATCTTTACTTC	138.51854
BC77303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77303	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77303_Pcan_Bot2011_IGS	S18-08	1	BC77303_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77303	Sample	BC77303	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-08	IGS	45	BC77303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-08	CGTAATCCTCAGTACTGGCTA	138.51854
BC77303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77303	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.158	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77303_Sspa_Oph2007_COI	S18-08	1	BC77303_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77303	Sample	BC77303	targeted	Present	Sample	Sspa_Oph2007_COI	S18-08	COI	45	BC77303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-08	GAAGGCCTTCCGATGATC	138.51854
BC77303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77303	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77303_Upin_Bot2011_COI	S18-08	1	BC77303_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77303	Sample	BC77303	targeted	Absent	Sample	Upin_Bot2011_COI	S18-08	COI	45	BC77303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51854
BC77304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81006	WGS84	BC77304	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77304_Cjap_Sim2018_COI	S18-09	1	BC77304_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77304	Sample	BC77304	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-09	COI	45	BC77304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51952
BC77304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81006	WGS84	BC77304	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.104	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77304_Mgig_Bot2012_COI	S18-09	1	BC77304_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77304	Sample	BC77304	targeted	Present	Sample	Mgig_Bot2012_COI	S18-09	COI	45	BC77304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51952
BC77305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81013	WGS84	BC77305	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77305_Cmae_Bot2011_COI	S18-10	1	BC77305_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77305	Sample	BC77305	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-10	COI	45	BC77305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-10	ATGAACAGTCTATCCTCCTTTAG	138.5198
BC77307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77357	WGS84	BC77307	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77307_Aamu_Bax2006_COI	S19-02	1	BC77307_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77307	Sample	BC77307	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-02	COI	45	BC77307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-02	GCACAACCGGGATCTTTACTTC	138.48591
BC77307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77357	WGS84	BC77307	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77307_Pcan_Bot2011_IGS	S19-02	1	BC77307_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77307	Sample	BC77307	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-02	IGS	45	BC77307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-02	CGTAATCCTCAGTACTGGCTA	138.48591
BC77307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77357	WGS84	BC77307	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.734	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77307_Sspa_Oph2007_COI	S19-02	1	BC77307_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77307	Sample	BC77307	targeted	Present	Sample	Sspa_Oph2007_COI	S19-02	COI	45	BC77307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-02	GAAGGCCTTCCGATGATC	138.48591
BC77307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77357	WGS84	BC77307	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77307_Upin_Bot2011_COI	S19-02	1	BC77307_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77307	Sample	BC77307	targeted	Absent	Sample	Upin_Bot2011_COI	S19-02	COI	45	BC77307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.48591
BC77308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77295	WGS84	BC77308	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77308_Cjap_Sim2018_COI	S19-03	1	BC77308_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77308	Sample	BC77308	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-03	COI	45	BC77308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48449
BC77308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77295	WGS84	BC77308	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77308_Mgig_Bot2012_COI	S19-03	1	BC77308_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77308	Sample	BC77308	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-03	COI	45	BC77308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.48449
BC77309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77781	WGS84	BC77309	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77309_Cmae_Bot2011_COI	S19-04	1	BC77309_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77309	Sample	BC77309	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-04	COI	45	BC77309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-04	ATGAACAGTCTATCCTCCTTTAG	138.51905
BC77310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7798	WGS84	BC77310	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77310_Aamu_Bax2006_COI	S19-05	1	BC77310_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77310	Sample	BC77310	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-05	COI	45	BC77310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-05	GCACAACCGGGATCTTTACTTC	138.51801
BC77310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7798	WGS84	BC77310	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77310_Pcan_Bot2011_IGS	S19-05	1	BC77310_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77310	Sample	BC77310	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-05	IGS	45	BC77310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-05	CGTAATCCTCAGTACTGGCTA	138.51801
BC77310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7798	WGS84	BC77310	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.131	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77310_Sspa_Oph2007_COI	S19-05	1	BC77310_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77310	Sample	BC77310	targeted	Present	Sample	Sspa_Oph2007_COI	S19-05	COI	45	BC77310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-05	GAAGGCCTTCCGATGATC	138.51801
BC77310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7798	WGS84	BC77310	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77310_Upin_Bot2011_COI	S19-05	1	BC77310_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77310	Sample	BC77310	targeted	Absent	Sample	Upin_Bot2011_COI	S19-05	COI	45	BC77310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.51801
BC77311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78118	WGS84	BC77311	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77311_Cjap_Sim2018_COI	S19-06	1	BC77311_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77311	Sample	BC77311	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-06	COI	45	BC77311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5172
BC77311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78118	WGS84	BC77311	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77311_Mgig_Bot2012_COI	S19-06	1	BC77311_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77311	Sample	BC77311	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-06	COI	45	BC77311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.5172
BC77312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84678	WGS84	BC77312	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77312_Cmae_Bot2011_COI	S19-07	1	BC77312_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77312	Sample	BC77312	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-07	COI	45	BC77312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-07	ATGAACAGTCTATCCTCCTTTAG	138.49755
BC77314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84334	WGS84	BC77314	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77314_Aamu_Bax2006_COI	S19-09	1	BC77314_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77314	Sample	BC77314	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-09	COI	45	BC77314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-09	GCACAACCGGGATCTTTACTTC	138.49677
BC77314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84334	WGS84	BC77314	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77314_Pcan_Bot2011_IGS	S19-09	1	BC77314_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77314	Sample	BC77314	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-09	IGS	45	BC77314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-09	CGTAATCCTCAGTACTGGCTA	138.49677
BC77314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84334	WGS84	BC77314	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.036	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77314_Sspa_Oph2007_COI	S19-09	1	BC77314_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77314	Sample	BC77314	targeted	Present	Sample	Sspa_Oph2007_COI	S19-09	COI	45	BC77314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-09	GAAGGCCTTCCGATGATC	138.49677
BC77314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84334	WGS84	BC77314	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77314_Upin_Bot2011_COI	S19-09	1	BC77314_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77314	Sample	BC77314	targeted	Absent	Sample	Upin_Bot2011_COI	S19-09	COI	45	BC77314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.49677
BC77315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83658	WGS84	BC77315	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77315_Cjap_Sim2018_COI	S19-10	1	BC77315_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77315	Sample	BC77315	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-10	COI	45	BC77315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50835
BC77315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83658	WGS84	BC77315	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77315_Mgig_Bot2012_COI	S19-10	1	BC77315_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77315	Sample	BC77315	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-10	COI	45	BC77315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.50835
BC77316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83711	WGS84	BC77316	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77316_Cmae_Bot2011_COI	S19-11	1	BC77316_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77316	Sample	BC77316	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-11	COI	45	BC77316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-11	ATGAACAGTCTATCCTCCTTTAG	138.50972
BC77318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8092	WGS84	BC77318	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77318_Aamu_Bax2006_COI	S19-13	1	BC77318_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77318	Sample	BC77318	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-13	COI	45	BC77318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-13	GCACAACCGGGATCTTTACTTC	138.5177
BC77318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8092	WGS84	BC77318	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77318_Pcan_Bot2011_IGS	S19-13	1	BC77318_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77318	Sample	BC77318	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-13	IGS	45	BC77318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-13	CGTAATCCTCAGTACTGGCTA	138.5177
BC77318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8092	WGS84	BC77318	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.76	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77318_Sspa_Oph2007_COI	S19-13	1	BC77318_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77318	Sample	BC77318	targeted	Present	Sample	Sspa_Oph2007_COI	S19-13	COI	45	BC77318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-13	GAAGGCCTTCCGATGATC	138.5177
BC77318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8092	WGS84	BC77318	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77318_Upin_Bot2011_COI	S19-13	1	BC77318_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77318	Sample	BC77318	targeted	Absent	Sample	Upin_Bot2011_COI	S19-13	COI	45	BC77318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.5177
BC77319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81009	WGS84	BC77319	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77319_Cjap_Sim2018_COI	S19-14	1	BC77319_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77319	Sample	BC77319	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-14	COI	45	BC77319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52
BC77319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81009	WGS84	BC77319	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.981	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77319_Mgig_Bot2012_COI	S19-14	1	BC77319_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77319	Sample	BC77319	targeted	Present	Sample	Mgig_Bot2012_COI	S19-14	COI	45	BC77319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.52
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28890_Cmae_Bot2011_COI	POR-B29	1	BD28890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	ATGAACAGTCTATCCTCCTTTAG	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28890_Hsan_Wil2021_COI	POR-B29	1	BD28890_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	CCTGGGCCGGTATAGTAGGT	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28890_Mros_Gib2022_COI	POR-B29	1	BD28890_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28890_Msal_Bot2012_COI	POR-B29	1	BD28890_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	GYTAGTTCCRATGATGTTAGCTG	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28890_Mstr_Wil2021_COI	POR-B29	1	BD28890_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	GTAGTGGTAACTACTCATGCTTTA	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28890_Pvir_Dia2013_COI	POR-B29	1	BD28890_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	CTTAGTGGCATTAATTCGDAATCC	141.62107
BD28890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34647	WGS84	BD28890	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28890_Rhar_Sim2018_COI	POR-B29	1	BD28890_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28890	Sample	BD28890	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B29	COI	45	BD28890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B29	CTGCTGCTATTGCTCATG	141.62107
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28892_Aamu_Bax2006_COI	POR-B31	1	BD28892_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	GCACAACCGGGATCTTTACTTC	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28892_Pcan_Bot2011_IGS	POR-B31	1	BD28892_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B31	IGS	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	CGTAATCCTCAGTACTGGCTA	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28892_Pper_Dia2013_COI	POR-B31	1	BD28892_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	CTTAGTGGCATTAATTCGDAATCC	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28892_Sspa_Oph2007_COI	POR-B31	1	BD28892_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	GAAGGCCTTCCGATGATC	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28892_Upin_Bot2011_COI	POR-B31	1	BD28892_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	CTTTAATTACAGCGTTTTTATTGTTGT	141.61982
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28893_Cjap_Sim2018_COI	POR-B32	1	BD28893_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28893_Esin_And2018_Cyb	POR-B32	1	BD28893_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B32	Cytb	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	ACCCCTCCTCATATCCAACCA	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28893_Mgig_Bot2012_COI	POR-B32	1	BD28893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	TCTTATTCGTTGGAGACTTTATAACCCT	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28893_Mjap_Gib2022_COI	POR-B32	1	BD28893_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	AATTTAATTGTGACTAGGCATGGTCTG	141.62082
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28894_Cmae_Bot2011_COI	POR-B33	1	BD28894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	ATGAACAGTCTATCCTCCTTTAG	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28894_Hsan_Wil2021_COI	POR-B33	1	BD28894_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	CCTGGGCCGGTATAGTAGGT	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28894_Mros_Gib2022_COI	POR-B33	1	BD28894_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28894_Msal_Bot2012_COI	POR-B33	1	BD28894_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	GYTAGTTCCRATGATGTTAGCTG	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28894_Mstr_Wil2021_COI	POR-B33	1	BD28894_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	GTAGTGGTAACTACTCATGCTTTA	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28894_Pvir_Dia2013_COI	POR-B33	1	BD28894_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	CTTAGTGGCATTAATTCGDAATCC	141.61708
BD28894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28894	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28894_Rhar_Sim2018_COI	POR-B33	1	BD28894_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28894	Sample	BD28894	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B33	COI	45	BD28894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B33	CTGCTGCTATTGCTCATG	141.61708
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28896_Aamu_Bax2006_COI	POR-B22	1	BD28896_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	GCACAACCGGGATCTTTACTTC	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28896_Pcan_Bot2011_IGS	POR-B22	1	BD28896_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B22	IGS	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	CGTAATCCTCAGTACTGGCTA	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28896_Pper_Dia2013_COI	POR-B22	1	BD28896_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	CTTAGTGGCATTAATTCGDAATCC	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28896_Sspa_Oph2007_COI	POR-B22	1	BD28896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	GAAGGCCTTCCGATGATC	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28896_Upin_Bot2011_COI	POR-B22	1	BD28896_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	CTTTAATTACAGCGTTTTTATTGTTGT	141.62235
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28897_Cjap_Sim2018_COI	POR-B21	1	BD28897_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28897_Esin_And2018_Cyb	POR-B21	1	BD28897_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B21	Cytb	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	ACCCCTCCTCATATCCAACCA	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28897_Mgig_Bot2012_COI	POR-B21	1	BD28897_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	TCTTATTCGTTGGAGACTTTATAACCCT	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28897_Mjap_Gib2022_COI	POR-B21	1	BD28897_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	AATTTAATTGTGACTAGGCATGGTCTG	141.62176
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28898_Cmae_Bot2011_COI	POR-B20	1	BD28898_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	ATGAACAGTCTATCCTCCTTTAG	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28898_Hsan_Wil2021_COI	POR-B20	1	BD28898_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	CCTGGGCCGGTATAGTAGGT	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28898_Mros_Gib2022_COI	POR-B20	1	BD28898_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28898_Msal_Bot2012_COI	POR-B20	1	BD28898_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	GYTAGTTCCRATGATGTTAGCTG	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28898_Mstr_Wil2021_COI	POR-B20	1	BD28898_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	GTAGTGGTAACTACTCATGCTTTA	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28898_Pvir_Dia2013_COI	POR-B20	1	BD28898_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	CTTAGTGGCATTAATTCGDAATCC	141.62028
BD28898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD28898	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28898_Rhar_Sim2018_COI	POR-B20	1	BD28898_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28898	Sample	BD28898	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B20	COI	45	BD28898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B20	CTGCTGCTATTGCTCATG	141.62028
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28900_Aamu_Bax2006_COI	POR-B18	1	BD28900_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	GCACAACCGGGATCTTTACTTC	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28900_Pcan_Bot2011_IGS	POR-B18	1	BD28900_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B18	IGS	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	CGTAATCCTCAGTACTGGCTA	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28900_Pper_Dia2013_COI	POR-B18	1	BD28900_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	CTTAGTGGCATTAATTCGDAATCC	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28900_Sspa_Oph2007_COI	POR-B18	1	BD28900_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	GAAGGCCTTCCGATGATC	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.626	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28900_Upin_Bot2011_COI	POR-B18	1	BD28900_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Present	Sample	Upin_Bot2011_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	CTTTAATTACAGCGTTTTTATTGTTGT	141.61759
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28901_Cjap_Sim2018_COI	POR-B17	1	BD28901_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28901_Esin_And2018_Cyb	POR-B17	1	BD28901_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B17	Cytb	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	ACCCCTCCTCATATCCAACCA	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28901_Mgig_Bot2012_COI	POR-B17	1	BD28901_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	TCTTATTCGTTGGAGACTTTATAACCCT	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28901_Mjap_Gib2022_COI	POR-B17	1	BD28901_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	AATTTAATTGTGACTAGGCATGGTCTG	141.61616
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28902_Cmae_Bot2011_COI	POR-B16	1	BD28902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	ATGAACAGTCTATCCTCCTTTAG	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28902_Hsan_Wil2021_COI	POR-B16	1	BD28902_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	CCTGGGCCGGTATAGTAGGT	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28902_Mros_Gib2022_COI	POR-B16	1	BD28902_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28902_Msal_Bot2012_COI	POR-B16	1	BD28902_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	GYTAGTTCCRATGATGTTAGCTG	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28902_Mstr_Wil2021_COI	POR-B16	1	BD28902_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	GTAGTGGTAACTACTCATGCTTTA	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28902_Pvir_Dia2013_COI	POR-B16	1	BD28902_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	CTTAGTGGCATTAATTCGDAATCC	141.6148
BD28902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34582	WGS84	BD28902	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28902_Rhar_Sim2018_COI	POR-B16	1	BD28902_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28902	Sample	BD28902	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B16	COI	45	BD28902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B16	CTGCTGCTATTGCTCATG	141.6148
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28904_Aamu_Bax2006_COI	POR-B14	1	BD28904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	GCACAACCGGGATCTTTACTTC	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28904_Pcan_Bot2011_IGS	POR-B14	1	BD28904_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B14	IGS	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	CGTAATCCTCAGTACTGGCTA	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28904_Pper_Dia2013_COI	POR-B14	1	BD28904_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	CTTAGTGGCATTAATTCGDAATCC	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28904_Sspa_Oph2007_COI	POR-B14	1	BD28904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	GAAGGCCTTCCGATGATC	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28904_Upin_Bot2011_COI	POR-B14	1	BD28904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	CTTTAATTACAGCGTTTTTATTGTTGT	141.61401
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28905_Cjap_Sim2018_COI	POR-B13	1	BD28905_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28905_Esin_And2018_Cyb	POR-B13	1	BD28905_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B13	Cytb	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	ACCCCTCCTCATATCCAACCA	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28905_Mgig_Bot2012_COI	POR-B13	1	BD28905_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	TCTTATTCGTTGGAGACTTTATAACCCT	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28905_Mjap_Gib2022_COI	POR-B13	1	BD28905_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	AATTTAATTGTGACTAGGCATGGTCTG	141.61265
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28906_Cmae_Bot2011_COI	POR-B12	1	BD28906_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	ATGAACAGTCTATCCTCCTTTAG	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28906_Hsan_Wil2021_COI	POR-B12	1	BD28906_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	CCTGGGCCGGTATAGTAGGT	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28906_Mros_Gib2022_COI	POR-B12	1	BD28906_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28906_Msal_Bot2012_COI	POR-B12	1	BD28906_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	GYTAGTTCCRATGATGTTAGCTG	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28906_Mstr_Wil2021_COI	POR-B12	1	BD28906_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	GTAGTGGTAACTACTCATGCTTTA	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28906_Pvir_Dia2013_COI	POR-B12	1	BD28906_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	CTTAGTGGCATTAATTCGDAATCC	141.61129
BD28906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34498	WGS84	BD28906	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28906_Rhar_Sim2018_COI	POR-B12	1	BD28906_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28906	Sample	BD28906	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B12	COI	45	BD28906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B12	CTGCTGCTATTGCTCATG	141.61129
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28909_Cjap_Sim2018_COI	POR-B10	1	BD28909_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28909_Esin_And2018_Cyb	POR-B10	1	BD28909_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B10	Cytb	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	ACCCCTCCTCATATCCAACCA	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28909_Mgig_Bot2012_COI	POR-B10	1	BD28909_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	TCTTATTCGTTGGAGACTTTATAACCCT	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28909_Mjap_Gib2022_COI	POR-B10	1	BD28909_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	AATTTAATTGTGACTAGGCATGGTCTG	141.62026
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28911_Aamu_Bax2006_COI	POR-B08	1	BD28911_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	GCACAACCGGGATCTTTACTTC	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28911_Pcan_Bot2011_IGS	POR-B08	1	BD28911_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B08	IGS	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	CGTAATCCTCAGTACTGGCTA	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28911_Pper_Dia2013_COI	POR-B08	1	BD28911_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	CTTAGTGGCATTAATTCGDAATCC	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28911_Sspa_Oph2007_COI	POR-B08	1	BD28911_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	GAAGGCCTTCCGATGATC	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28911_Upin_Bot2011_COI	POR-B08	1	BD28911_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	CTTTAATTACAGCGTTTTTATTGTTGT	141.61753
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28912_Cjap_Sim2018_COI	POR-B07	1	BD28912_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28912_Esin_And2018_Cyb	POR-B07	1	BD28912_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B07	Cytb	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	ACCCCTCCTCATATCCAACCA	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28912_Mgig_Bot2012_COI	POR-B07	1	BD28912_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	TCTTATTCGTTGGAGACTTTATAACCCT	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28912_Mjap_Gib2022_COI	POR-B07	1	BD28912_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	AATTTAATTGTGACTAGGCATGGTCTG	141.61615
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28913_Cmae_Bot2011_COI	POR-B06	1	BD28913_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	ATGAACAGTCTATCCTCCTTTAG	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28913_Hsan_Wil2021_COI	POR-B06	1	BD28913_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	CCTGGGCCGGTATAGTAGGT	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28913_Mros_Gib2022_COI	POR-B06	1	BD28913_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28913_Msal_Bot2012_COI	POR-B06	1	BD28913_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	GYTAGTTCCRATGATGTTAGCTG	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28913_Mstr_Wil2021_COI	POR-B06	1	BD28913_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	GTAGTGGTAACTACTCATGCTTTA	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28913_Pvir_Dia2013_COI	POR-B06	1	BD28913_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	CTTAGTGGCATTAATTCGDAATCC	141.61473
BD28913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34761	WGS84	BD28913	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28913_Rhar_Sim2018_COI	POR-B06	1	BD28913_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28913	Sample	BD28913	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B06	COI	45	BD28913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B06	CTGCTGCTATTGCTCATG	141.61473
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28915_Aamu_Bax2006_COI	POR-B04	1	BD28915_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	GCACAACCGGGATCTTTACTTC	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28915_Pcan_Bot2011_IGS	POR-B04	1	BD28915_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B04	IGS	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	CGTAATCCTCAGTACTGGCTA	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28915_Pper_Dia2013_COI	POR-B04	1	BD28915_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	CTTAGTGGCATTAATTCGDAATCC	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.679	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28915_Sspa_Oph2007_COI	POR-B04	1	BD28915_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Present	Sample	Sspa_Oph2007_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	GAAGGCCTTCCGATGATC	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.539	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28915_Upin_Bot2011_COI	POR-B04	1	BD28915_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Present	Sample	Upin_Bot2011_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	CTTTAATTACAGCGTTTTTATTGTTGT	141.61159
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28916_Cjap_Sim2018_COI	POR-B03	1	BD28916_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28916_Esin_And2018_Cyb	POR-B03	1	BD28916_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B03	Cytb	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	ACCCCTCCTCATATCCAACCA	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28916_Mgig_Bot2012_COI	POR-B03	1	BD28916_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	TCTTATTCGTTGGAGACTTTATAACCCT	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28916_Mjap_Gib2022_COI	POR-B03	1	BD28916_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	AATTTAATTGTGACTAGGCATGGTCTG	141.61129
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28917_Cmae_Bot2011_COI	POR-B02	1	BD28917_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	ATGAACAGTCTATCCTCCTTTAG	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28917_Hsan_Wil2021_COI	POR-B02	1	BD28917_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	CCTGGGCCGGTATAGTAGGT	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.515	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28917_Mros_Gib2022_COI	POR-B02	1	BD28917_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Present	Sample	Mros_Gib2022_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28917_Msal_Bot2012_COI	POR-B02	1	BD28917_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	GYTAGTTCCRATGATGTTAGCTG	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28917_Mstr_Wil2021_COI	POR-B02	1	BD28917_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	GTAGTGGTAACTACTCATGCTTTA	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28917_Pvir_Dia2013_COI	POR-B02	1	BD28917_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	CTTAGTGGCATTAATTCGDAATCC	141.61065
BD28917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34782	WGS84	BD28917	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28917_Rhar_Sim2018_COI	POR-B02	1	BD28917_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28917	Sample	BD28917	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B02	COI	45	BD28917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B02	CTGCTGCTATTGCTCATG	141.61065
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28919_Aamu_Bax2006_COI	ADL-B35	1	BD28919_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	GCACAACCGGGATCTTTACTTC	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28919_Pcan_Bot2011_IGS	ADL-B35	1	BD28919_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B35	IGS	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	CGTAATCCTCAGTACTGGCTA	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28919_Pper_Dia2013_COI	ADL-B35	1	BD28919_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	CTTAGTGGCATTAATTCGDAATCC	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28919_Sspa_Oph2007_COI	ADL-B35	1	BD28919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	GAAGGCCTTCCGATGATC	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28919_Upin_Bot2011_COI	ADL-B35	1	BD28919_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	CTTTAATTACAGCGTTTTTATTGTTGT	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81115	WGS84	BD28919	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28919_Vgib_Wil2025_COI	ADL-B35	1	BD28919_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	CCCCTCTTTACAAGTTCTTTAGGGT	138.51463
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28920_Cmae_Bot2011_COI	ADL-B34	1	BD28920_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	ATGAACAGTCTATCCTCCTTTAG	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28920_Hsan_Wil2021_COI	ADL-B34	1	BD28920_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	CCTGGGCCGGTATAGTAGGT	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28920_Mros_Gib2022_COI	ADL-B34	1	BD28920_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28920_Msal_Bot2012_COI	ADL-B34	1	BD28920_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	GYTAGTTCCRATGATGTTAGCTG	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28920_Mstr_Wil2021_COI	ADL-B34	1	BD28920_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	GTAGTGGTAACTACTCATGCTTTA	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28920_Pvir_Dia2013_COI	ADL-B34	1	BD28920_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	CTTAGTGGCATTAATTCGDAATCC	138.514
BD28920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81218	WGS84	BD28920	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28920_Rhar_Sim2018_COI	ADL-B34	1	BD28920_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28920	Sample	BD28920	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B34	COI	45	BD28920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B34	CTGCTGCTATTGCTCATG	138.514
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28922_Aamu_Bax2006_COI	ADL-B32	1	BD28922_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	GCACAACCGGGATCTTTACTTC	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28922_Pcan_Bot2011_IGS	ADL-B32	1	BD28922_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B32	IGS	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	CGTAATCCTCAGTACTGGCTA	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28922_Pper_Dia2013_COI	ADL-B32	1	BD28922_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	CTTAGTGGCATTAATTCGDAATCC	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.636	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28922_Sspa_Oph2007_COI	ADL-B32	1	BD28922_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	GAAGGCCTTCCGATGATC	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28922_Upin_Bot2011_COI	ADL-B32	1	BD28922_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	CTTTAATTACAGCGTTTTTATTGTTGT	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81518	WGS84	BD28922	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28922_Vgib_Wil2025_COI	ADL-B32	1	BD28922_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	CCCCTCTTTACAAGTTCTTTAGGGT	138.51323
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81627	WGS84	BD28923	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28923_Asen_Wil2025_COI	ADL-B31	1	BD28923_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	GTTGTTGTTACAACACATGCTCTTTTG	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28923_Cjap_Sim2018_COI	ADL-B31	1	BD28923_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28923_Esin_And2018_Cyb	ADL-B31	1	BD28923_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B31	Cytb	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	ACCCCTCCTCATATCCAACCA	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.297	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28923_Mgig_Bot2012_COI	ADL-B31	1	BD28923_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	TCTTATTCGTTGGAGACTTTATAACCCT	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28923_Mjap_Gib2022_COI	ADL-B31	1	BD28923_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	AATTTAATTGTGACTAGGCATGGTCTG	138.51405
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28924_Cmae_Bot2011_COI	ADL-B30	1	BD28924_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	ATGAACAGTCTATCCTCCTTTAG	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28924_Hsan_Wil2021_COI	ADL-B30	1	BD28924_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	CCTGGGCCGGTATAGTAGGT	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28924_Mros_Gib2022_COI	ADL-B30	1	BD28924_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28924_Msal_Bot2012_COI	ADL-B30	1	BD28924_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	GYTAGTTCCRATGATGTTAGCTG	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28924_Mstr_Wil2021_COI	ADL-B30	1	BD28924_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	GTAGTGGTAACTACTCATGCTTTA	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28924_Pvir_Dia2013_COI	ADL-B30	1	BD28924_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	CTTAGTGGCATTAATTCGDAATCC	138.51328
BD28924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81728	WGS84	BD28924	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28924_Rhar_Sim2018_COI	ADL-B30	1	BD28924_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28924	Sample	BD28924	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B30	COI	45	BD28924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B30	CTGCTGCTATTGCTCATG	138.51328
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28926_Aamu_Bax2006_COI	ADL-B28	1	BD28926_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	GCACAACCGGGATCTTTACTTC	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28926_Pcan_Bot2011_IGS	ADL-B28	1	BD28926_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B28	IGS	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	CGTAATCCTCAGTACTGGCTA	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28926_Pper_Dia2013_COI	ADL-B28	1	BD28926_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	CTTAGTGGCATTAATTCGDAATCC	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28926_Sspa_Oph2007_COI	ADL-B28	1	BD28926_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	GAAGGCCTTCCGATGATC	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28926_Upin_Bot2011_COI	ADL-B28	1	BD28926_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	CTTTAATTACAGCGTTTTTATTGTTGT	138.51205
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28927_Cjap_Sim2018_COI	ADL-B27	1	BD28927_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28927_Esin_And2018_Cyb	ADL-B27	1	BD28927_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B27	Cytb	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	ACCCCTCCTCATATCCAACCA	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.621	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28927_Mgig_Bot2012_COI	ADL-B27	1	BD28927_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	TCTTATTCGTTGGAGACTTTATAACCCT	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28927_Mjap_Gib2022_COI	ADL-B27	1	BD28927_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	AATTTAATTGTGACTAGGCATGGTCTG	138.51146
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28928_Cmae_Bot2011_COI	ADL-B26	1	BD28928_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	ATGAACAGTCTATCCTCCTTTAG	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28928_Hsan_Wil2021_COI	ADL-B26	1	BD28928_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	CCTGGGCCGGTATAGTAGGT	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28928_Mros_Gib2022_COI	ADL-B26	1	BD28928_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28928_Msal_Bot2012_COI	ADL-B26	1	BD28928_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	GYTAGTTCCRATGATGTTAGCTG	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28928_Mstr_Wil2021_COI	ADL-B26	1	BD28928_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	GTAGTGGTAACTACTCATGCTTTA	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28928_Pvir_Dia2013_COI	ADL-B26	1	BD28928_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	CTTAGTGGCATTAATTCGDAATCC	138.51074
BD28928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82143	WGS84	BD28928	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28928_Rhar_Sim2018_COI	ADL-B26	1	BD28928_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28928	Sample	BD28928	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B26	COI	45	BD28928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B26	CTGCTGCTATTGCTCATG	138.51074
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28930_Cjap_Sim2018_COI	ADL-B24	1	BD28930_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28930_Esin_And2018_Cyb	ADL-B24	1	BD28930_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B24	Cytb	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	ACCCCTCCTCATATCCAACCA	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.367	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28930_Mgig_Bot2012_COI	ADL-B24	1	BD28930_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	TCTTATTCGTTGGAGACTTTATAACCCT	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28930_Mjap_Gib2022_COI	ADL-B24	1	BD28930_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	AATTTAATTGTGACTAGGCATGGTCTG	138.51033
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28931_Cmae_Bot2011_COI	ADL-B23	1	BD28931_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	ATGAACAGTCTATCCTCCTTTAG	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28931_Hsan_Wil2021_COI	ADL-B23	1	BD28931_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	CCTGGGCCGGTATAGTAGGT	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28931_Mros_Gib2022_COI	ADL-B23	1	BD28931_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28931_Msal_Bot2012_COI	ADL-B23	1	BD28931_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	GYTAGTTCCRATGATGTTAGCTG	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28931_Mstr_Wil2021_COI	ADL-B23	1	BD28931_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	GTAGTGGTAACTACTCATGCTTTA	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28931_Pvir_Dia2013_COI	ADL-B23	1	BD28931_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	CTTAGTGGCATTAATTCGDAATCC	138.51105
BD28931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82357	WGS84	BD28931	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28931_Rhar_Sim2018_COI	ADL-B23	1	BD28931_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28931	Sample	BD28931	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B23	COI	45	BD28931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B23	CTGCTGCTATTGCTCATG	138.51105
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28933_Aamu_Bax2006_COI	ADL-B21	1	BD28933_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	GCACAACCGGGATCTTTACTTC	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28933_Pcan_Bot2011_IGS	ADL-B21	1	BD28933_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B21	IGS	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	CGTAATCCTCAGTACTGGCTA	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28933_Pper_Dia2013_COI	ADL-B21	1	BD28933_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	CTTAGTGGCATTAATTCGDAATCC	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28933_Sspa_Oph2007_COI	ADL-B21	1	BD28933_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	GAAGGCCTTCCGATGATC	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28933_Upin_Bot2011_COI	ADL-B21	1	BD28933_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	CTTTAATTACAGCGTTTTTATTGTTGT	138.51072
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28934_Cjap_Sim2018_COI	ADL-B20	1	BD28934_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28934_Esin_And2018_Cyb	ADL-B20	1	BD28934_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B20	Cytb	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	ACCCCTCCTCATATCCAACCA	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.716	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28934_Mgig_Bot2012_COI	ADL-B20	1	BD28934_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	TCTTATTCGTTGGAGACTTTATAACCCT	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28934_Mjap_Gib2022_COI	ADL-B20	1	BD28934_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	AATTTAATTGTGACTAGGCATGGTCTG	138.50884
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28935_Cmae_Bot2011_COI	ADL-B19	1	BD28935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	ATGAACAGTCTATCCTCCTTTAG	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28935_Hsan_Wil2021_COI	ADL-B19	1	BD28935_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	CCTGGGCCGGTATAGTAGGT	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28935_Mros_Gib2022_COI	ADL-B19	1	BD28935_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28935_Msal_Bot2012_COI	ADL-B19	1	BD28935_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	GYTAGTTCCRATGATGTTAGCTG	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28935_Mstr_Wil2021_COI	ADL-B19	1	BD28935_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	GTAGTGGTAACTACTCATGCTTTA	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28935_Pvir_Dia2013_COI	ADL-B19	1	BD28935_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	CTTAGTGGCATTAATTCGDAATCC	138.50973
BD28935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82565	WGS84	BD28935	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28935_Rhar_Sim2018_COI	ADL-B19	1	BD28935_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28935	Sample	BD28935	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B19	COI	45	BD28935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B19	CTGCTGCTATTGCTCATG	138.50973
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28937_Aamu_Bax2006_COI	ADL-B17	1	BD28937_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	GCACAACCGGGATCTTTACTTC	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28937_Pcan_Bot2011_IGS	ADL-B17	1	BD28937_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B17	IGS	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	CGTAATCCTCAGTACTGGCTA	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28937_Pper_Dia2013_COI	ADL-B17	1	BD28937_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	CTTAGTGGCATTAATTCGDAATCC	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.989	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28937_Sspa_Oph2007_COI	ADL-B17	1	BD28937_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	GAAGGCCTTCCGATGATC	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28937_Upin_Bot2011_COI	ADL-B17	1	BD28937_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	CTTTAATTACAGCGTTTTTATTGTTGT	138.50901
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28938_Cjap_Sim2018_COI	ADL-B16	1	BD28938_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28938_Esin_And2018_Cyb	ADL-B16	1	BD28938_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B16	Cytb	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	ACCCCTCCTCATATCCAACCA	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.393	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28938_Mgig_Bot2012_COI	ADL-B16	1	BD28938_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	TCTTATTCGTTGGAGACTTTATAACCCT	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28938_Mjap_Gib2022_COI	ADL-B16	1	BD28938_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	AATTTAATTGTGACTAGGCATGGTCTG	138.50756
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28939_Cmae_Bot2011_COI	ADL-B15	1	BD28939_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	ATGAACAGTCTATCCTCCTTTAG	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28939_Hsan_Wil2021_COI	ADL-B15	1	BD28939_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	CCTGGGCCGGTATAGTAGGT	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28939_Mros_Gib2022_COI	ADL-B15	1	BD28939_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28939_Msal_Bot2012_COI	ADL-B15	1	BD28939_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	GYTAGTTCCRATGATGTTAGCTG	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28939_Mstr_Wil2021_COI	ADL-B15	1	BD28939_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	GTAGTGGTAACTACTCATGCTTTA	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28939_Pvir_Dia2013_COI	ADL-B15	1	BD28939_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	CTTAGTGGCATTAATTCGDAATCC	138.50806
BD28939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82762	WGS84	BD28939	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28939_Rhar_Sim2018_COI	ADL-B15	1	BD28939_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28939	Sample	BD28939	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B15	COI	45	BD28939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B15	CTGCTGCTATTGCTCATG	138.50806
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28940_Aamu_Bax2006_COI	ADL-B14	1	BD28940_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	GCACAACCGGGATCTTTACTTC	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28940_Pcan_Bot2011_IGS	ADL-B14	1	BD28940_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B14	IGS	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	CGTAATCCTCAGTACTGGCTA	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28940_Pper_Dia2013_COI	ADL-B14	1	BD28940_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	CTTAGTGGCATTAATTCGDAATCC	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28940_Sspa_Oph2007_COI	ADL-B14	1	BD28940_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	GAAGGCCTTCCGATGATC	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28940_Upin_Bot2011_COI	ADL-B14	1	BD28940_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	CTTTAATTACAGCGTTTTTATTGTTGT	138.50873
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28941_Cjap_Sim2018_COI	ADL-B13	1	BD28941_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28941_Esin_And2018_Cyb	ADL-B13	1	BD28941_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B13	Cytb	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	ACCCCTCCTCATATCCAACCA	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.351	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28941_Mgig_Bot2012_COI	ADL-B13	1	BD28941_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	TCTTATTCGTTGGAGACTTTATAACCCT	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28941_Mjap_Gib2022_COI	ADL-B13	1	BD28941_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	AATTTAATTGTGACTAGGCATGGTCTG	138.50754
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28942_Cmae_Bot2011_COI	ADL-B12	1	BD28942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	ATGAACAGTCTATCCTCCTTTAG	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28942_Hsan_Wil2021_COI	ADL-B12	1	BD28942_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	CCTGGGCCGGTATAGTAGGT	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28942_Mros_Gib2022_COI	ADL-B12	1	BD28942_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28942_Msal_Bot2012_COI	ADL-B12	1	BD28942_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	GYTAGTTCCRATGATGTTAGCTG	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28942_Mstr_Wil2021_COI	ADL-B12	1	BD28942_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	GTAGTGGTAACTACTCATGCTTTA	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28942_Pvir_Dia2013_COI	ADL-B12	1	BD28942_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	CTTAGTGGCATTAATTCGDAATCC	138.5093
BD28942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.8296	WGS84	BD28942	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28942_Rhar_Sim2018_COI	ADL-B12	1	BD28942_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28942	Sample	BD28942	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B12	COI	45	BD28942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B12	CTGCTGCTATTGCTCATG	138.5093
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28944_Aamu_Bax2006_COI	ADL-B10	1	BD28944_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	GCACAACCGGGATCTTTACTTC	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28944_Pcan_Bot2011_IGS	ADL-B10	1	BD28944_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B10	IGS	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	CGTAATCCTCAGTACTGGCTA	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28944_Pper_Dia2013_COI	ADL-B10	1	BD28944_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	CTTAGTGGCATTAATTCGDAATCC	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28944_Sspa_Oph2007_COI	ADL-B10	1	BD28944_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	GAAGGCCTTCCGATGATC	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28944_Upin_Bot2011_COI	ADL-B10	1	BD28944_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	CTTTAATTACAGCGTTTTTATTGTTGT	138.50761
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28945_Cjap_Sim2018_COI	ADL-B09	1	BD28945_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28945_Esin_And2018_Cyb	ADL-B09	1	BD28945_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B09	Cytb	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	ACCCCTCCTCATATCCAACCA	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.478	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28945_Mgig_Bot2012_COI	ADL-B09	1	BD28945_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	TCTTATTCGTTGGAGACTTTATAACCCT	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28945_Mjap_Gib2022_COI	ADL-B09	1	BD28945_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	AATTTAATTGTGACTAGGCATGGTCTG	138.50754
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28946_Cmae_Bot2011_COI	ADL-B08	1	BD28946_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	ATGAACAGTCTATCCTCCTTTAG	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28946_Hsan_Wil2021_COI	ADL-B08	1	BD28946_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	CCTGGGCCGGTATAGTAGGT	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28946_Mros_Gib2022_COI	ADL-B08	1	BD28946_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28946_Msal_Bot2012_COI	ADL-B08	1	BD28946_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	GYTAGTTCCRATGATGTTAGCTG	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28946_Mstr_Wil2021_COI	ADL-B08	1	BD28946_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	GTAGTGGTAACTACTCATGCTTTA	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28946_Pvir_Dia2013_COI	ADL-B08	1	BD28946_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	CTTAGTGGCATTAATTCGDAATCC	138.51028
BD28946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83709	WGS84	BD28946	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28946_Rhar_Sim2018_COI	ADL-B08	1	BD28946_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28946	Sample	BD28946	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B08	COI	45	BD28946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B08	CTGCTGCTATTGCTCATG	138.51028
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28948_Aamu_Bax2006_COI	ADL-B06	1	BD28948_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	GCACAACCGGGATCTTTACTTC	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28948_Pcan_Bot2011_IGS	ADL-B06	1	BD28948_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B06	IGS	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	CGTAATCCTCAGTACTGGCTA	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28948_Pper_Dia2013_COI	ADL-B06	1	BD28948_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	CTTAGTGGCATTAATTCGDAATCC	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28948_Sspa_Oph2007_COI	ADL-B06	1	BD28948_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	GAAGGCCTTCCGATGATC	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28948_Upin_Bot2011_COI	ADL-B06	1	BD28948_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	CTTTAATTACAGCGTTTTTATTGTTGT	138.50663
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28949_Cjap_Sim2018_COI	ADL-B05	1	BD28949_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28949_Esin_And2018_Cyb	ADL-B05	1	BD28949_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B05	Cytb	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	ACCCCTCCTCATATCCAACCA	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.269	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28949_Mgig_Bot2012_COI	ADL-B05	1	BD28949_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	TCTTATTCGTTGGAGACTTTATAACCCT	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28949_Mjap_Gib2022_COI	ADL-B05	1	BD28949_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	AATTTAATTGTGACTAGGCATGGTCTG	138.50549
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28951_Aamu_Bax2006_COI	ADL-B03	1	BD28951_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	GCACAACCGGGATCTTTACTTC	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28951_Pcan_Bot2011_IGS	ADL-B03	1	BD28951_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B03	IGS	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	CGTAATCCTCAGTACTGGCTA	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28951_Pper_Dia2013_COI	ADL-B03	1	BD28951_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	CTTAGTGGCATTAATTCGDAATCC	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28951_Sspa_Oph2007_COI	ADL-B03	1	BD28951_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	GAAGGCCTTCCGATGATC	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28951_Upin_Bot2011_COI	ADL-B03	1	BD28951_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	CTTTAATTACAGCGTTTTTATTGTTGT	138.50558
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28952_Cjap_Sim2018_COI	ADL-B02	1	BD28952_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28952_Esin_And2018_Cyb	ADL-B02	1	BD28952_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B02	Cytb	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	ACCCCTCCTCATATCCAACCA	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.083	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28952_Mgig_Bot2012_COI	ADL-B02	1	BD28952_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	TCTTATTCGTTGGAGACTTTATAACCCT	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28952_Mjap_Gib2022_COI	ADL-B02	1	BD28952_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	AATTTAATTGTGACTAGGCATGGTCTG	138.50066
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28953_Cmae_Bot2011_COI	ADL-B01	1	BD28953_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	ATGAACAGTCTATCCTCCTTTAG	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28953_Hsan_Wil2021_COI	ADL-B01	1	BD28953_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	CCTGGGCCGGTATAGTAGGT	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28953_Mros_Gib2022_COI	ADL-B01	1	BD28953_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28953_Msal_Bot2012_COI	ADL-B01	1	BD28953_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	GYTAGTTCCRATGATGTTAGCTG	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28953_Mstr_Wil2021_COI	ADL-B01	1	BD28953_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	GTAGTGGTAACTACTCATGCTTTA	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28953_Pvir_Dia2013_COI	ADL-B01	1	BD28953_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	CTTAGTGGCATTAATTCGDAATCC	138.49924
BD28953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84255	WGS84	BD28953	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28953_Rhar_Sim2018_COI	ADL-B01	1	BD28953_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28953	Sample	BD28953	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B01	COI	45	BD28953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B01	CTGCTGCTATTGCTCATG	138.49924
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28955_Aamu_Bax2006_COI	ADL-B69	1	BD28955_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	GCACAACCGGGATCTTTACTTC	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28955_Pcan_Bot2011_IGS	ADL-B69	1	BD28955_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B69	IGS	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	CGTAATCCTCAGTACTGGCTA	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28955_Pper_Dia2013_COI	ADL-B69	1	BD28955_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	CTTAGTGGCATTAATTCGDAATCC	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28955_Sspa_Oph2007_COI	ADL-B69	1	BD28955_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	GAAGGCCTTCCGATGATC	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28955_Upin_Bot2011_COI	ADL-B69	1	BD28955_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	CTTTAATTACAGCGTTTTTATTGTTGT	138.49891
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28956_Cjap_Sim2018_COI	ADL-B68	1	BD28956_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28956_Esin_And2018_Cyb	ADL-B68	1	BD28956_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B68	Cytb	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	ACCCCTCCTCATATCCAACCA	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.794	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28956_Mgig_Bot2012_COI	ADL-B68	1	BD28956_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	TCTTATTCGTTGGAGACTTTATAACCCT	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28956_Mjap_Gib2022_COI	ADL-B68	1	BD28956_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	AATTTAATTGTGACTAGGCATGGTCTG	138.50173
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28957_Cmae_Bot2011_COI	ADL-B67	1	BD28957_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	ATGAACAGTCTATCCTCCTTTAG	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28957_Hsan_Wil2021_COI	ADL-B67	1	BD28957_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	CCTGGGCCGGTATAGTAGGT	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28957_Mros_Gib2022_COI	ADL-B67	1	BD28957_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28957_Msal_Bot2012_COI	ADL-B67	1	BD28957_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	GYTAGTTCCRATGATGTTAGCTG	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28957_Mstr_Wil2021_COI	ADL-B67	1	BD28957_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	GTAGTGGTAACTACTCATGCTTTA	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28957_Pvir_Dia2013_COI	ADL-B67	1	BD28957_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	CTTAGTGGCATTAATTCGDAATCC	138.49964
BD28957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7622	WGS84	BD28957	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28957_Rhar_Sim2018_COI	ADL-B67	1	BD28957_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28957	Sample	BD28957	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B67	COI	45	BD28957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B67	CTGCTGCTATTGCTCATG	138.49964
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28959_Aamu_Bax2006_COI	ADL-B65	1	BD28959_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	GCACAACCGGGATCTTTACTTC	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28959_Pcan_Bot2011_IGS	ADL-B65	1	BD28959_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B65	IGS	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	CGTAATCCTCAGTACTGGCTA	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28959_Pper_Dia2013_COI	ADL-B65	1	BD28959_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	CTTAGTGGCATTAATTCGDAATCC	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.185	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28959_Sspa_Oph2007_COI	ADL-B65	1	BD28959_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	GAAGGCCTTCCGATGATC	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28959_Upin_Bot2011_COI	ADL-B65	1	BD28959_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	CTTTAATTACAGCGTTTTTATTGTTGT	138.49536
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28960_Cmae_Bot2011_COI	ADL-B64	1	BD28960_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	ATGAACAGTCTATCCTCCTTTAG	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28960_Hsan_Wil2021_COI	ADL-B64	1	BD28960_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	CCTGGGCCGGTATAGTAGGT	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28960_Mros_Gib2022_COI	ADL-B64	1	BD28960_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28960_Msal_Bot2012_COI	ADL-B64	1	BD28960_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	GYTAGTTCCRATGATGTTAGCTG	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28960_Mstr_Wil2021_COI	ADL-B64	1	BD28960_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	GTAGTGGTAACTACTCATGCTTTA	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28960_Pvir_Dia2013_COI	ADL-B64	1	BD28960_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	CTTAGTGGCATTAATTCGDAATCC	138.49652
BD28960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76384	WGS84	BD28960	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28960_Rhar_Sim2018_COI	ADL-B64	1	BD28960_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28960	Sample	BD28960	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B64	COI	45	BD28960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B64	CTGCTGCTATTGCTCATG	138.49652
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28962_Aamu_Bax2006_COI	ADL-B62	1	BD28962_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	GCACAACCGGGATCTTTACTTC	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28962_Pcan_Bot2011_IGS	ADL-B62	1	BD28962_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B62	IGS	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	CGTAATCCTCAGTACTGGCTA	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28962_Pper_Dia2013_COI	ADL-B62	1	BD28962_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	CTTAGTGGCATTAATTCGDAATCC	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28962_Sspa_Oph2007_COI	ADL-B62	1	BD28962_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	GAAGGCCTTCCGATGATC	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28962_Upin_Bot2011_COI	ADL-B62	1	BD28962_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	CTTTAATTACAGCGTTTTTATTGTTGT	138.49227
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28963_Cjap_Sim2018_COI	ADL-B61	1	BD28963_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28963_Esin_And2018_Cyb	ADL-B61	1	BD28963_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B61	Cytb	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	ACCCCTCCTCATATCCAACCA	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.487	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28963_Mgig_Bot2012_COI	ADL-B61	1	BD28963_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	TCTTATTCGTTGGAGACTTTATAACCCT	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28963_Mjap_Gib2022_COI	ADL-B61	1	BD28963_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	AATTTAATTGTGACTAGGCATGGTCTG	138.49346
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28964_Cmae_Bot2011_COI	ADL-B60	1	BD28964_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	ATGAACAGTCTATCCTCCTTTAG	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28964_Hsan_Wil2021_COI	ADL-B60	1	BD28964_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	CCTGGGCCGGTATAGTAGGT	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28964_Mros_Gib2022_COI	ADL-B60	1	BD28964_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28964_Msal_Bot2012_COI	ADL-B60	1	BD28964_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	GYTAGTTCCRATGATGTTAGCTG	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28964_Mstr_Wil2021_COI	ADL-B60	1	BD28964_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	GTAGTGGTAACTACTCATGCTTTA	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28964_Pvir_Dia2013_COI	ADL-B60	1	BD28964_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	CTTAGTGGCATTAATTCGDAATCC	138.49128
BD28964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76547	WGS84	BD28964	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28964_Rhar_Sim2018_COI	ADL-B60	1	BD28964_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28964	Sample	BD28964	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B60	COI	45	BD28964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B60	CTGCTGCTATTGCTCATG	138.49128
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28966_Aamu_Bax2006_COI	ADL-B58	1	BD28966_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	GCACAACCGGGATCTTTACTTC	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28966_Pcan_Bot2011_IGS	ADL-B58	1	BD28966_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B58	IGS	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	CGTAATCCTCAGTACTGGCTA	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28966_Pper_Dia2013_COI	ADL-B58	1	BD28966_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	CTTAGTGGCATTAATTCGDAATCC	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28966_Sspa_Oph2007_COI	ADL-B58	1	BD28966_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	GAAGGCCTTCCGATGATC	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28966_Upin_Bot2011_COI	ADL-B58	1	BD28966_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	CTTTAATTACAGCGTTTTTATTGTTGT	138.48822
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28967_Cjap_Sim2018_COI	ADL-B57	1	BD28967_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28967_Esin_And2018_Cyb	ADL-B57	1	BD28967_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B57	Cytb	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	ACCCCTCCTCATATCCAACCA	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.621	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28967_Mgig_Bot2012_COI	ADL-B57	1	BD28967_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	TCTTATTCGTTGGAGACTTTATAACCCT	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28967_Mjap_Gib2022_COI	ADL-B57	1	BD28967_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	AATTTAATTGTGACTAGGCATGGTCTG	138.48711
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28968_Cmae_Bot2011_COI	ADL-B56	1	BD28968_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	ATGAACAGTCTATCCTCCTTTAG	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28968_Hsan_Wil2021_COI	ADL-B56	1	BD28968_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	CCTGGGCCGGTATAGTAGGT	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28968_Mros_Gib2022_COI	ADL-B56	1	BD28968_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28968_Msal_Bot2012_COI	ADL-B56	1	BD28968_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	GYTAGTTCCRATGATGTTAGCTG	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28968_Mstr_Wil2021_COI	ADL-B56	1	BD28968_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	GTAGTGGTAACTACTCATGCTTTA	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28968_Pvir_Dia2013_COI	ADL-B56	1	BD28968_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	CTTAGTGGCATTAATTCGDAATCC	138.48611
BD28968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76703	WGS84	BD28968	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28968_Rhar_Sim2018_COI	ADL-B56	1	BD28968_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28968	Sample	BD28968	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B56	COI	45	BD28968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B56	CTGCTGCTATTGCTCATG	138.48611
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28970_Cjap_Sim2018_COI	ADL-B54	1	BD28970_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28970_Esin_And2018_Cyb	ADL-B54	1	BD28970_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B54	Cytb	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	ACCCCTCCTCATATCCAACCA	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.863	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28970_Mgig_Bot2012_COI	ADL-B54	1	BD28970_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	TCTTATTCGTTGGAGACTTTATAACCCT	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28970_Mjap_Gib2022_COI	ADL-B54	1	BD28970_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	AATTTAATTGTGACTAGGCATGGTCTG	138.48724
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28971_Cmae_Bot2011_COI	ADL-B53	1	BD28971_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	ATGAACAGTCTATCCTCCTTTAG	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28971_Hsan_Wil2021_COI	ADL-B53	1	BD28971_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	CCTGGGCCGGTATAGTAGGT	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28971_Mros_Gib2022_COI	ADL-B53	1	BD28971_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28971_Msal_Bot2012_COI	ADL-B53	1	BD28971_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	GYTAGTTCCRATGATGTTAGCTG	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28971_Mstr_Wil2021_COI	ADL-B53	1	BD28971_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	GTAGTGGTAACTACTCATGCTTTA	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28971_Pvir_Dia2013_COI	ADL-B53	1	BD28971_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	CTTAGTGGCATTAATTCGDAATCC	138.48516
BD28971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76868	WGS84	BD28971	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28971_Rhar_Sim2018_COI	ADL-B53	1	BD28971_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28971	Sample	BD28971	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B53	COI	45	BD28971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B53	CTGCTGCTATTGCTCATG	138.48516
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28973_Aamu_Bax2006_COI	ADL-B51	1	BD28973_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	GCACAACCGGGATCTTTACTTC	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28973_Pcan_Bot2011_IGS	ADL-B51	1	BD28973_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B51	IGS	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	CGTAATCCTCAGTACTGGCTA	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28973_Pper_Dia2013_COI	ADL-B51	1	BD28973_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	CTTAGTGGCATTAATTCGDAATCC	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.808	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28973_Sspa_Oph2007_COI	ADL-B51	1	BD28973_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	GAAGGCCTTCCGATGATC	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28973_Upin_Bot2011_COI	ADL-B51	1	BD28973_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	CTTTAATTACAGCGTTTTTATTGTTGT	138.48404
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28974_Cjap_Sim2018_COI	ADL-B50	1	BD28974_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28974_Esin_And2018_Cyb	ADL-B50	1	BD28974_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B50	Cytb	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	ACCCCTCCTCATATCCAACCA	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.343	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28974_Mgig_Bot2012_COI	ADL-B50	1	BD28974_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	TCTTATTCGTTGGAGACTTTATAACCCT	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28974_Mjap_Gib2022_COI	ADL-B50	1	BD28974_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	AATTTAATTGTGACTAGGCATGGTCTG	138.48525
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28975_Cmae_Bot2011_COI	ADL-B49	1	BD28975_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	ATGAACAGTCTATCCTCCTTTAG	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28975_Hsan_Wil2021_COI	ADL-B49	1	BD28975_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	CCTGGGCCGGTATAGTAGGT	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28975_Mros_Gib2022_COI	ADL-B49	1	BD28975_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28975_Msal_Bot2012_COI	ADL-B49	1	BD28975_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	GYTAGTTCCRATGATGTTAGCTG	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28975_Mstr_Wil2021_COI	ADL-B49	1	BD28975_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	GTAGTGGTAACTACTCATGCTTTA	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28975_Pvir_Dia2013_COI	ADL-B49	1	BD28975_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	CTTAGTGGCATTAATTCGDAATCC	138.48311
BD28975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77177	WGS84	BD28975	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28975_Rhar_Sim2018_COI	ADL-B49	1	BD28975_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28975	Sample	BD28975	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B49	COI	45	BD28975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B49	CTGCTGCTATTGCTCATG	138.48311
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28977_Aamu_Bax2006_COI	ADL-B47	1	BD28977_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	GCACAACCGGGATCTTTACTTC	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28977_Pcan_Bot2011_IGS	ADL-B47	1	BD28977_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B47	IGS	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	CGTAATCCTCAGTACTGGCTA	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28977_Pper_Dia2013_COI	ADL-B47	1	BD28977_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	CTTAGTGGCATTAATTCGDAATCC	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28977_Sspa_Oph2007_COI	ADL-B47	1	BD28977_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	GAAGGCCTTCCGATGATC	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28977_Upin_Bot2011_COI	ADL-B47	1	BD28977_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	CTTTAATTACAGCGTTTTTATTGTTGT	138.48203
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77474	WGS84	BD28978	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28978_Asen_Wil2025_COI	ADL-B46	1	BD28978_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	GTTGTTGTTACAACACATGCTCTTTTG	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28978_Cjap_Sim2018_COI	ADL-B46	1	BD28978_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28978_Esin_And2018_Cyb	ADL-B46	1	BD28978_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B46	Cytb	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	ACCCCTCCTCATATCCAACCA	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.772	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28978_Mgig_Bot2012_COI	ADL-B46	1	BD28978_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	TCTTATTCGTTGGAGACTTTATAACCCT	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28978_Mjap_Gib2022_COI	ADL-B46	1	BD28978_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	AATTTAATTGTGACTAGGCATGGTCTG	138.48334
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28979_Cmae_Bot2011_COI	ADL-B45	1	BD28979_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	ATGAACAGTCTATCCTCCTTTAG	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28979_Hsan_Wil2021_COI	ADL-B45	1	BD28979_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	CCTGGGCCGGTATAGTAGGT	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28979_Mros_Gib2022_COI	ADL-B45	1	BD28979_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28979_Msal_Bot2012_COI	ADL-B45	1	BD28979_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	GYTAGTTCCRATGATGTTAGCTG	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28979_Mstr_Wil2021_COI	ADL-B45	1	BD28979_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	GTAGTGGTAACTACTCATGCTTTA	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28979_Pvir_Dia2013_COI	ADL-B45	1	BD28979_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	CTTAGTGGCATTAATTCGDAATCC	138.48234
BD28979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77618	WGS84	BD28979	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28979_Rhar_Sim2018_COI	ADL-B45	1	BD28979_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28979	Sample	BD28979	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B45	COI	45	BD28979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B45	CTGCTGCTATTGCTCATG	138.48234
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28980_Aamu_Bax2006_COI	ADL-B44	1	BD28980_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	GCACAACCGGGATCTTTACTTC	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28980_Pcan_Bot2011_IGS	ADL-B44	1	BD28980_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B44	IGS	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	CGTAATCCTCAGTACTGGCTA	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28980_Pper_Dia2013_COI	ADL-B44	1	BD28980_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	CTTAGTGGCATTAATTCGDAATCC	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28980_Sspa_Oph2007_COI	ADL-B44	1	BD28980_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	GAAGGCCTTCCGATGATC	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28980_Upin_Bot2011_COI	ADL-B44	1	BD28980_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	CTTTAATTACAGCGTTTTTATTGTTGT	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77483	WGS84	BD28980	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28980_Vgib_Wil2025_COI	ADL-B44	1	BD28980_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	CCCCTCTTTACAAGTTCTTTAGGGT	138.48112
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77623	WGS84	BD28981	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28981_Asen_Wil2025_COI	ADL-B43	1	BD28981_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	GTTGTTGTTACAACACATGCTCTTTTG	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28981_Cjap_Sim2018_COI	ADL-B43	1	BD28981_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28981_Esin_And2018_Cyb	ADL-B43	1	BD28981_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B43	Cytb	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	ACCCCTCCTCATATCCAACCA	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28981_Mgig_Bot2012_COI	ADL-B43	1	BD28981_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	TCTTATTCGTTGGAGACTTTATAACCCT	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28981_Mjap_Gib2022_COI	ADL-B43	1	BD28981_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	AATTTAATTGTGACTAGGCATGGTCTG	138.4802
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28982_Cmae_Bot2011_COI	ADL-B42	1	BD28982_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	ATGAACAGTCTATCCTCCTTTAG	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28982_Hsan_Wil2021_COI	ADL-B42	1	BD28982_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	CCTGGGCCGGTATAGTAGGT	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28982_Mros_Gib2022_COI	ADL-B42	1	BD28982_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28982_Msal_Bot2012_COI	ADL-B42	1	BD28982_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	GYTAGTTCCRATGATGTTAGCTG	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28982_Mstr_Wil2021_COI	ADL-B42	1	BD28982_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	GTAGTGGTAACTACTCATGCTTTA	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28982_Pvir_Dia2013_COI	ADL-B42	1	BD28982_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	CTTAGTGGCATTAATTCGDAATCC	138.47916
BD28982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77789	WGS84	BD28982	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28982_Rhar_Sim2018_COI	ADL-B42	1	BD28982_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28982	Sample	BD28982	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B42	COI	45	BD28982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B42	CTGCTGCTATTGCTCATG	138.47916
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28984_Aamu_Bax2006_COI	ADL-B40	1	BD28984_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	GCACAACCGGGATCTTTACTTC	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28984_Pcan_Bot2011_IGS	ADL-B40	1	BD28984_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B40	IGS	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	CGTAATCCTCAGTACTGGCTA	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28984_Pper_Dia2013_COI	ADL-B40	1	BD28984_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	CTTAGTGGCATTAATTCGDAATCC	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28984_Sspa_Oph2007_COI	ADL-B40	1	BD28984_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	GAAGGCCTTCCGATGATC	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28984_Upin_Bot2011_COI	ADL-B40	1	BD28984_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	CTTTAATTACAGCGTTTTTATTGTTGT	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78099	WGS84	BD28984	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28984_Vgib_Wil2025_COI	ADL-B40	1	BD28984_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	CCCCTCTTTACAAGTTCTTTAGGGT	138.47714
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78257	WGS84	BD28985	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28985_Asen_Wil2025_COI	ADL-B39	1	BD28985_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	GTTGTTGTTACAACACATGCTCTTTTG	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28985_Cjap_Sim2018_COI	ADL-B39	1	BD28985_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28985_Esin_And2018_Cyb	ADL-B39	1	BD28985_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B39	Cytb	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	ACCCCTCCTCATATCCAACCA	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.667	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28985_Mgig_Bot2012_COI	ADL-B39	1	BD28985_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	TCTTATTCGTTGGAGACTTTATAACCCT	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28985_Mjap_Gib2022_COI	ADL-B39	1	BD28985_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	AATTTAATTGTGACTAGGCATGGTCTG	138.47624
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28986_Cmae_Bot2011_COI	ADL-B38	1	BD28986_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	ATGAACAGTCTATCCTCCTTTAG	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28986_Hsan_Wil2021_COI	ADL-B38	1	BD28986_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	CCTGGGCCGGTATAGTAGGT	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28986_Mros_Gib2022_COI	ADL-B38	1	BD28986_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28986_Msal_Bot2012_COI	ADL-B38	1	BD28986_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	GYTAGTTCCRATGATGTTAGCTG	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28986_Mstr_Wil2021_COI	ADL-B38	1	BD28986_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	GTAGTGGTAACTACTCATGCTTTA	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28986_Pvir_Dia2013_COI	ADL-B38	1	BD28986_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	CTTAGTGGCATTAATTCGDAATCC	138.47417
BD28986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78276	WGS84	BD28986	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28986_Rhar_Sim2018_COI	ADL-B38	1	BD28986_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28986	Sample	BD28986	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B38	COI	45	BD28986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B38	CTGCTGCTATTGCTCATG	138.47417
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28988_Aamu_Bax2006_COI	ADL-B36	1	BD28988_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	GCACAACCGGGATCTTTACTTC	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28988_Pcan_Bot2011_IGS	ADL-B36	1	BD28988_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B36	IGS	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	CGTAATCCTCAGTACTGGCTA	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28988_Pper_Dia2013_COI	ADL-B36	1	BD28988_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	CTTAGTGGCATTAATTCGDAATCC	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28988_Sspa_Oph2007_COI	ADL-B36	1	BD28988_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	GAAGGCCTTCCGATGATC	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28988_Upin_Bot2011_COI	ADL-B36	1	BD28988_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	CTTTAATTACAGCGTTTTTATTGTTGT	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78394	WGS84	BD28988	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28988_Vgib_Wil2025_COI	ADL-B36	1	BD28988_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	CCCCTCTTTACAAGTTCTTTAGGGT	138.47309
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28989_Cjap_Sim2018_COI	PLO-B40	1	BD28989_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28989_Esin_And2018_Cyb	PLO-B40	1	BD28989_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B40	Cytb	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	ACCCCTCCTCATATCCAACCA	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28989_Mgig_Bot2012_COI	PLO-B40	1	BD28989_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	TCTTATTCGTTGGAGACTTTATAACCCT	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28989_Mjap_Gib2022_COI	PLO-B40	1	BD28989_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	AATTTAATTGTGACTAGGCATGGTCTG	135.86778
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28991_Aamu_Bax2006_COI	PLO-B38	1	BD28991_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	GCACAACCGGGATCTTTACTTC	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28991_Pcan_Bot2011_IGS	PLO-B38	1	BD28991_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B38	IGS	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	CGTAATCCTCAGTACTGGCTA	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28991_Pper_Dia2013_COI	PLO-B38	1	BD28991_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	CTTAGTGGCATTAATTCGDAATCC	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.343	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28991_Sspa_Oph2007_COI	PLO-B38	1	BD28991_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	GAAGGCCTTCCGATGATC	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28991_Upin_Bot2011_COI	PLO-B38	1	BD28991_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	CTTTAATTACAGCGTTTTTATTGTTGT	135.87269
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28992_Cjap_Sim2018_COI	PLO-B37	1	BD28992_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28992_Esin_And2018_Cyb	PLO-B37	1	BD28992_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B37	Cytb	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	ACCCCTCCTCATATCCAACCA	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28992_Mgig_Bot2012_COI	PLO-B37	1	BD28992_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	TCTTATTCGTTGGAGACTTTATAACCCT	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28992_Mjap_Gib2022_COI	PLO-B37	1	BD28992_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	AATTTAATTGTGACTAGGCATGGTCTG	135.87379
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28993_Cmae_Bot2011_COI	PLO-B36	1	BD28993_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	ATGAACAGTCTATCCTCCTTTAG	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28993_Hsan_Wil2021_COI	PLO-B36	1	BD28993_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	CCTGGGCCGGTATAGTAGGT	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28993_Mros_Gib2022_COI	PLO-B36	1	BD28993_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28993_Msal_Bot2012_COI	PLO-B36	1	BD28993_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	GYTAGTTCCRATGATGTTAGCTG	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28993_Mstr_Wil2021_COI	PLO-B36	1	BD28993_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	GTAGTGGTAACTACTCATGCTTTA	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28993_Pvir_Dia2013_COI	PLO-B36	1	BD28993_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	CTTAGTGGCATTAATTCGDAATCC	135.87749
BD28993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74193	WGS84	BD28993	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28993_Rhar_Sim2018_COI	PLO-B36	1	BD28993_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28993	Sample	BD28993	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B36	COI	45	BD28993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B36	CTGCTGCTATTGCTCATG	135.87749
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28995_Aamu_Bax2006_COI	THE-B34	1	BD28995_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	GCACAACCGGGATCTTTACTTC	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28995_Pcan_Bot2011_IGS	THE-B34	1	BD28995_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B34	IGS	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	CGTAATCCTCAGTACTGGCTA	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28995_Pper_Dia2013_COI	THE-B34	1	BD28995_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	CTTAGTGGCATTAATTCGDAATCC	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28995_Sspa_Oph2007_COI	THE-B34	1	BD28995_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	GAAGGCCTTCCGATGATC	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28995_Upin_Bot2011_COI	THE-B34	1	BD28995_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	CTTTAATTACAGCGTTTTTATTGTTGT	133.63995
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28996_Cjap_Sim2018_COI	THE-B33	1	BD28996_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28996_Esin_And2018_Cyb	THE-B33	1	BD28996_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B33	Cytb	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	ACCCCTCCTCATATCCAACCA	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.407	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28996_Mgig_Bot2012_COI	THE-B33	1	BD28996_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	TCTTATTCGTTGGAGACTTTATAACCCT	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28996_Mjap_Gib2022_COI	THE-B33	1	BD28996_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	AATTTAATTGTGACTAGGCATGGTCTG	133.64047
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28997_Cmae_Bot2011_COI	THE-B32	1	BD28997_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	ATGAACAGTCTATCCTCCTTTAG	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28997_Hsan_Wil2021_COI	THE-B32	1	BD28997_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	CCTGGGCCGGTATAGTAGGT	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28997_Mros_Gib2022_COI	THE-B32	1	BD28997_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28997_Msal_Bot2012_COI	THE-B32	1	BD28997_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	GYTAGTTCCRATGATGTTAGCTG	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28997_Mstr_Wil2021_COI	THE-B32	1	BD28997_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	GTAGTGGTAACTACTCATGCTTTA	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28997_Pvir_Dia2013_COI	THE-B32	1	BD28997_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	CTTAGTGGCATTAATTCGDAATCC	133.63493
BD28997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.1468	WGS84	BD28997	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28997_Rhar_Sim2018_COI	THE-B32	1	BD28997_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28997	Sample	BD28997	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B32	COI	45	BD28997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B32	CTGCTGCTATTGCTCATG	133.63493
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28999_Aamu_Bax2006_COI	THE-B30	1	BD28999_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	GCACAACCGGGATCTTTACTTC	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28999_Pcan_Bot2011_IGS	THE-B30	1	BD28999_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B30	IGS	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	CGTAATCCTCAGTACTGGCTA	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28999_Pper_Dia2013_COI	THE-B30	1	BD28999_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	CTTAGTGGCATTAATTCGDAATCC	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28999_Sspa_Oph2007_COI	THE-B30	1	BD28999_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	GAAGGCCTTCCGATGATC	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28999_Upin_Bot2011_COI	THE-B30	1	BD28999_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	CTTTAATTACAGCGTTTTTATTGTTGT	133.63797
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29000_Cmae_Bot2011_COI	THE-B29	1	BD29000_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	ATGAACAGTCTATCCTCCTTTAG	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29000_Hsan_Wil2021_COI	THE-B29	1	BD29000_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	CCTGGGCCGGTATAGTAGGT	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29000_Mros_Gib2022_COI	THE-B29	1	BD29000_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29000_Msal_Bot2012_COI	THE-B29	1	BD29000_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	GYTAGTTCCRATGATGTTAGCTG	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29000_Mstr_Wil2021_COI	THE-B29	1	BD29000_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	GTAGTGGTAACTACTCATGCTTTA	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29000_Pvir_Dia2013_COI	THE-B29	1	BD29000_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	CTTAGTGGCATTAATTCGDAATCC	133.6387
BD29000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14679	WGS84	BD29000	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29000_Rhar_Sim2018_COI	THE-B29	1	BD29000_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29000	Sample	BD29000	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B29	COI	45	BD29000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B29	CTGCTGCTATTGCTCATG	133.6387
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29002_Aamu_Bax2006_COI	THE-B27	1	BD29002_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	GCACAACCGGGATCTTTACTTC	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29002_Pcan_Bot2011_IGS	THE-B27	1	BD29002_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B27	IGS	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	CGTAATCCTCAGTACTGGCTA	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29002_Pper_Dia2013_COI	THE-B27	1	BD29002_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	CTTAGTGGCATTAATTCGDAATCC	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29002_Sspa_Oph2007_COI	THE-B27	1	BD29002_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	GAAGGCCTTCCGATGATC	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29002_Upin_Bot2011_COI	THE-B27	1	BD29002_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	CTTTAATTACAGCGTTTTTATTGTTGT	133.64049
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29003_Cjap_Sim2018_COI	THE-B26	1	BD29003_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29003_Esin_And2018_Cyb	THE-B26	1	BD29003_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B26	Cytb	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	ACCCCTCCTCATATCCAACCA	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29003_Mgig_Bot2012_COI	THE-B26	1	BD29003_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	TCTTATTCGTTGGAGACTTTATAACCCT	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29003_Mjap_Gib2022_COI	THE-B26	1	BD29003_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	AATTTAATTGTGACTAGGCATGGTCTG	133.63596
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29004_Cmae_Bot2011_COI	THE-B25	1	BD29004_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	ATGAACAGTCTATCCTCCTTTAG	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29004_Hsan_Wil2021_COI	THE-B25	1	BD29004_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	CCTGGGCCGGTATAGTAGGT	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29004_Mros_Gib2022_COI	THE-B25	1	BD29004_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29004_Msal_Bot2012_COI	THE-B25	1	BD29004_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	GYTAGTTCCRATGATGTTAGCTG	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29004_Mstr_Wil2021_COI	THE-B25	1	BD29004_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	GTAGTGGTAACTACTCATGCTTTA	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29004_Pvir_Dia2013_COI	THE-B25	1	BD29004_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	CTTAGTGGCATTAATTCGDAATCC	133.63725
BD29004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14775	WGS84	BD29004	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29004_Rhar_Sim2018_COI	THE-B25	1	BD29004_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29004	Sample	BD29004	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B25	COI	45	BD29004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B25	CTGCTGCTATTGCTCATG	133.63725
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29006_Aamu_Bax2006_COI	THE-B23	1	BD29006_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	GCACAACCGGGATCTTTACTTC	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29006_Pcan_Bot2011_IGS	THE-B23	1	BD29006_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B23	IGS	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	CGTAATCCTCAGTACTGGCTA	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29006_Pper_Dia2013_COI	THE-B23	1	BD29006_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	CTTAGTGGCATTAATTCGDAATCC	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29006_Sspa_Oph2007_COI	THE-B23	1	BD29006_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	GAAGGCCTTCCGATGATC	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29006_Upin_Bot2011_COI	THE-B23	1	BD29006_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	CTTTAATTACAGCGTTTTTATTGTTGT	133.64018
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29007_Cjap_Sim2018_COI	THE-B22	1	BD29007_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29007_Esin_And2018_Cyb	THE-B22	1	BD29007_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B22	Cytb	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	ACCCCTCCTCATATCCAACCA	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.164	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29007_Mgig_Bot2012_COI	THE-B22	1	BD29007_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	TCTTATTCGTTGGAGACTTTATAACCCT	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29007_Mjap_Gib2022_COI	THE-B22	1	BD29007_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	AATTTAATTGTGACTAGGCATGGTCTG	133.64094
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29008_Cmae_Bot2011_COI	THE-B21	1	BD29008_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	ATGAACAGTCTATCCTCCTTTAG	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29008_Hsan_Wil2021_COI	THE-B21	1	BD29008_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	CCTGGGCCGGTATAGTAGGT	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29008_Mros_Gib2022_COI	THE-B21	1	BD29008_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29008_Msal_Bot2012_COI	THE-B21	1	BD29008_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	GYTAGTTCCRATGATGTTAGCTG	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29008_Mstr_Wil2021_COI	THE-B21	1	BD29008_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	GTAGTGGTAACTACTCATGCTTTA	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29008_Pvir_Dia2013_COI	THE-B21	1	BD29008_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	CTTAGTGGCATTAATTCGDAATCC	133.63514
BD29008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14892	WGS84	BD29008	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29008_Rhar_Sim2018_COI	THE-B21	1	BD29008_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29008	Sample	BD29008	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B21	COI	45	BD29008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B21	CTGCTGCTATTGCTCATG	133.63514
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.14915	WGS84	BD29010	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29010_Asen_Wil2025_COI	THE-B19	1	BD29010_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	GTTGTTGTTACAACACATGCTCTTTTG	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29010_Cjap_Sim2018_COI	THE-B19	1	BD29010_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29010_Esin_And2018_Cyb	THE-B19	1	BD29010_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B19	Cytb	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	ACCCCTCCTCATATCCAACCA	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.836	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29010_Mgig_Bot2012_COI	THE-B19	1	BD29010_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	TCTTATTCGTTGGAGACTTTATAACCCT	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29010_Mjap_Gib2022_COI	THE-B19	1	BD29010_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	AATTTAATTGTGACTAGGCATGGTCTG	133.63803
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29011_Cmae_Bot2011_COI	THE-B18	1	BD29011_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	ATGAACAGTCTATCCTCCTTTAG	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29011_Hsan_Wil2021_COI	THE-B18	1	BD29011_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	CCTGGGCCGGTATAGTAGGT	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29011_Mros_Gib2022_COI	THE-B18	1	BD29011_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29011_Msal_Bot2012_COI	THE-B18	1	BD29011_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	GYTAGTTCCRATGATGTTAGCTG	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29011_Mstr_Wil2021_COI	THE-B18	1	BD29011_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	GTAGTGGTAACTACTCATGCTTTA	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29011_Pvir_Dia2013_COI	THE-B18	1	BD29011_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	CTTAGTGGCATTAATTCGDAATCC	133.63787
BD29011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15349	WGS84	BD29011	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29011_Rhar_Sim2018_COI	THE-B18	1	BD29011_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29011	Sample	BD29011	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B18	COI	45	BD29011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B18	CTGCTGCTATTGCTCATG	133.63787
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29013_Aamu_Bax2006_COI	THE-B16	1	BD29013_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	GCACAACCGGGATCTTTACTTC	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29013_Pcan_Bot2011_IGS	THE-B16	1	BD29013_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B16	IGS	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	CGTAATCCTCAGTACTGGCTA	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29013_Pper_Dia2013_COI	THE-B16	1	BD29013_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	CTTAGTGGCATTAATTCGDAATCC	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29013_Sspa_Oph2007_COI	THE-B16	1	BD29013_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	GAAGGCCTTCCGATGATC	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29013_Upin_Bot2011_COI	THE-B16	1	BD29013_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	CTTTAATTACAGCGTTTTTATTGTTGT	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15244	WGS84	BD29013	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29013_Vgib_Wil2025_COI	THE-B16	1	BD29013_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	CCCCTCTTTACAAGTTCTTTAGGGT	133.64047
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15215	WGS84	BD29014	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29014_Asen_Wil2025_COI	THE-B15	1	BD29014_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	GTTGTTGTTACAACACATGCTCTTTTG	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29014_Cjap_Sim2018_COI	THE-B15	1	BD29014_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29014_Esin_And2018_Cyb	THE-B15	1	BD29014_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B15	Cytb	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	ACCCCTCCTCATATCCAACCA	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.069	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29014_Mgig_Bot2012_COI	THE-B15	1	BD29014_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	TCTTATTCGTTGGAGACTTTATAACCCT	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29014_Mjap_Gib2022_COI	THE-B15	1	BD29014_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	AATTTAATTGTGACTAGGCATGGTCTG	133.63988
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29015_Cmae_Bot2011_COI	THE-B14	1	BD29015_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	ATGAACAGTCTATCCTCCTTTAG	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29015_Hsan_Wil2021_COI	THE-B14	1	BD29015_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	CCTGGGCCGGTATAGTAGGT	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29015_Mros_Gib2022_COI	THE-B14	1	BD29015_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29015_Msal_Bot2012_COI	THE-B14	1	BD29015_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	GYTAGTTCCRATGATGTTAGCTG	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29015_Mstr_Wil2021_COI	THE-B14	1	BD29015_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	GTAGTGGTAACTACTCATGCTTTA	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29015_Pvir_Dia2013_COI	THE-B14	1	BD29015_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	CTTAGTGGCATTAATTCGDAATCC	133.63854
BD29015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15222	WGS84	BD29015	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29015_Rhar_Sim2018_COI	THE-B14	1	BD29015_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29015	Sample	BD29015	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B14	COI	45	BD29015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B14	CTGCTGCTATTGCTCATG	133.63854
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29017_Aamu_Bax2006_COI	THE-B12	1	BD29017_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	GCACAACCGGGATCTTTACTTC	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29017_Pcan_Bot2011_IGS	THE-B12	1	BD29017_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B12	IGS	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	CGTAATCCTCAGTACTGGCTA	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29017_Pper_Dia2013_COI	THE-B12	1	BD29017_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	CTTAGTGGCATTAATTCGDAATCC	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29017_Sspa_Oph2007_COI	THE-B12	1	BD29017_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	GAAGGCCTTCCGATGATC	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29017_Upin_Bot2011_COI	THE-B12	1	BD29017_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	CTTTAATTACAGCGTTTTTATTGTTGT	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15206	WGS84	BD29017	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29017_Vgib_Wil2025_COI	THE-B12	1	BD29017_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	CCCCTCTTTACAAGTTCTTTAGGGT	133.63559
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.151	WGS84	BD29018	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29018_Asen_Wil2025_COI	THE-B11	1	BD29018_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	GTTGTTGTTACAACACATGCTCTTTTG	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29018_Cjap_Sim2018_COI	THE-B11	1	BD29018_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29018_Esin_And2018_Cyb	THE-B11	1	BD29018_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B11	Cytb	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	ACCCCTCCTCATATCCAACCA	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.714	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29018_Mgig_Bot2012_COI	THE-B11	1	BD29018_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	TCTTATTCGTTGGAGACTTTATAACCCT	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29018_Mjap_Gib2022_COI	THE-B11	1	BD29018_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	AATTTAATTGTGACTAGGCATGGTCTG	133.6357
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29019_Cmae_Bot2011_COI	THE-B10	1	BD29019_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	ATGAACAGTCTATCCTCCTTTAG	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29019_Hsan_Wil2021_COI	THE-B10	1	BD29019_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	CCTGGGCCGGTATAGTAGGT	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29019_Mros_Gib2022_COI	THE-B10	1	BD29019_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29019_Msal_Bot2012_COI	THE-B10	1	BD29019_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	GYTAGTTCCRATGATGTTAGCTG	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29019_Mstr_Wil2021_COI	THE-B10	1	BD29019_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	GTAGTGGTAACTACTCATGCTTTA	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29019_Pvir_Dia2013_COI	THE-B10	1	BD29019_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	CTTAGTGGCATTAATTCGDAATCC	133.63644
BD29019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15113	WGS84	BD29019	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29019_Rhar_Sim2018_COI	THE-B10	1	BD29019_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29019	Sample	BD29019	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B10	COI	45	BD29019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B10	CTGCTGCTATTGCTCATG	133.63644
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29020_Aamu_Bax2006_COI	THE-B09	1	BD29020_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	GCACAACCGGGATCTTTACTTC	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29020_Pcan_Bot2011_IGS	THE-B09	1	BD29020_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B09	IGS	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	CGTAATCCTCAGTACTGGCTA	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29020_Pper_Dia2013_COI	THE-B09	1	BD29020_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	CTTAGTGGCATTAATTCGDAATCC	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29020_Sspa_Oph2007_COI	THE-B09	1	BD29020_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	GAAGGCCTTCCGATGATC	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29020_Upin_Bot2011_COI	THE-B09	1	BD29020_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	CTTTAATTACAGCGTTTTTATTGTTGT	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15116	WGS84	BD29020	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29020_Vgib_Wil2025_COI	THE-B09	1	BD29020_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	CCCCTCTTTACAAGTTCTTTAGGGT	133.63801
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15129	WGS84	BD29021	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29021_Asen_Wil2025_COI	THE-B08	1	BD29021_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	GTTGTTGTTACAACACATGCTCTTTTG	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29021_Cjap_Sim2018_COI	THE-B08	1	BD29021_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29021_Esin_And2018_Cyb	THE-B08	1	BD29021_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B08	Cytb	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	ACCCCTCCTCATATCCAACCA	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.002	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29021_Mgig_Bot2012_COI	THE-B08	1	BD29021_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	TCTTATTCGTTGGAGACTTTATAACCCT	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29021_Mjap_Gib2022_COI	THE-B08	1	BD29021_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	AATTTAATTGTGACTAGGCATGGTCTG	133.63934
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29022_Cmae_Bot2011_COI	THE-B07	1	BD29022_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	ATGAACAGTCTATCCTCCTTTAG	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29022_Hsan_Wil2021_COI	THE-B07	1	BD29022_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	CCTGGGCCGGTATAGTAGGT	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29022_Mros_Gib2022_COI	THE-B07	1	BD29022_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29022_Msal_Bot2012_COI	THE-B07	1	BD29022_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	GYTAGTTCCRATGATGTTAGCTG	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29022_Mstr_Wil2021_COI	THE-B07	1	BD29022_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	GTAGTGGTAACTACTCATGCTTTA	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29022_Pvir_Dia2013_COI	THE-B07	1	BD29022_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	CTTAGTGGCATTAATTCGDAATCC	133.64093
BD29022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15125	WGS84	BD29022	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29022_Rhar_Sim2018_COI	THE-B07	1	BD29022_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29022	Sample	BD29022	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B07	COI	45	BD29022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B07	CTGCTGCTATTGCTCATG	133.64093
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29024_Aamu_Bax2006_COI	THE-B05	1	BD29024_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	GCACAACCGGGATCTTTACTTC	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29024_Pcan_Bot2011_IGS	THE-B05	1	BD29024_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B05	IGS	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	CGTAATCCTCAGTACTGGCTA	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29024_Pper_Dia2013_COI	THE-B05	1	BD29024_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	CTTAGTGGCATTAATTCGDAATCC	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29024_Sspa_Oph2007_COI	THE-B05	1	BD29024_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	GAAGGCCTTCCGATGATC	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29024_Upin_Bot2011_COI	THE-B05	1	BD29024_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	CTTTAATTACAGCGTTTTTATTGTTGT	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15019	WGS84	BD29024	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29024_Vgib_Wil2025_COI	THE-B05	1	BD29024_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	CCCCTCTTTACAAGTTCTTTAGGGT	133.64111
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15014	WGS84	BD29025	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29025_Asen_Wil2025_COI	THE-B04	1	BD29025_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	GTTGTTGTTACAACACATGCTCTTTTG	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29025_Cjap_Sim2018_COI	THE-B04	1	BD29025_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29025_Esin_And2018_Cyb	THE-B04	1	BD29025_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B04	Cytb	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	ACCCCTCCTCATATCCAACCA	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.111	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29025_Mgig_Bot2012_COI	THE-B04	1	BD29025_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	TCTTATTCGTTGGAGACTTTATAACCCT	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29025_Mjap_Gib2022_COI	THE-B04	1	BD29025_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	AATTTAATTGTGACTAGGCATGGTCTG	133.63966
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29026_Cmae_Bot2011_COI	THE-B03	1	BD29026_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	ATGAACAGTCTATCCTCCTTTAG	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29026_Hsan_Wil2021_COI	THE-B03	1	BD29026_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	CCTGGGCCGGTATAGTAGGT	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29026_Mros_Gib2022_COI	THE-B03	1	BD29026_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29026_Msal_Bot2012_COI	THE-B03	1	BD29026_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	GYTAGTTCCRATGATGTTAGCTG	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29026_Mstr_Wil2021_COI	THE-B03	1	BD29026_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	GTAGTGGTAACTACTCATGCTTTA	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29026_Pvir_Dia2013_COI	THE-B03	1	BD29026_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	CTTAGTGGCATTAATTCGDAATCC	133.63801
BD29026	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15022	WGS84	BD29026	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29026_Rhar_Sim2018_COI	THE-B03	1	BD29026_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29026	Sample	BD29026	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B03	COI	45	BD29026	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B03	CTGCTGCTATTGCTCATG	133.63801
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29028_Aamu_Bax2006_COI	THE-B01	1	BD29028_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	GCACAACCGGGATCTTTACTTC	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29028_Pcan_Bot2011_IGS	THE-B01	1	BD29028_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B01	IGS	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	CGTAATCCTCAGTACTGGCTA	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29028_Pper_Dia2013_COI	THE-B01	1	BD29028_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	CTTAGTGGCATTAATTCGDAATCC	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29028_Sspa_Oph2007_COI	THE-B01	1	BD29028_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	GAAGGCCTTCCGATGATC	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29028_Upin_Bot2011_COI	THE-B01	1	BD29028_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	CTTTAATTACAGCGTTTTTATTGTTGT	133.63559
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29029_Cjap_Sim2018_COI	PGI-B35	1	BD29029_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29029_Esin_And2018_Cyb	PGI-B35	1	BD29029_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B35	Cytb	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	ACCCCTCCTCATATCCAACCA	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29029_Mgig_Bot2012_COI	PGI-B35	1	BD29029_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	TCTTATTCGTTGGAGACTTTATAACCCT	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29029_Mjap_Gib2022_COI	PGI-B35	1	BD29029_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	AATTTAATTGTGACTAGGCATGGTCTG	137.7706
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29031_Aamu_Bax2006_COI	PGI-B33	1	BD29031_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	GCACAACCGGGATCTTTACTTC	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29031_Pcan_Bot2011_IGS	PGI-B33	1	BD29031_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B33	IGS	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	CGTAATCCTCAGTACTGGCTA	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29031_Pper_Dia2013_COI	PGI-B33	1	BD29031_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	CTTAGTGGCATTAATTCGDAATCC	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29031_Sspa_Oph2007_COI	PGI-B33	1	BD29031_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	GAAGGCCTTCCGATGATC	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29031_Upin_Bot2011_COI	PGI-B33	1	BD29031_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	CTTTAATTACAGCGTTTTTATTGTTGT	137.76708
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29032_Cjap_Sim2018_COI	PGI-B32	1	BD29032_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29032_Esin_And2018_Cyb	PGI-B32	1	BD29032_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B32	Cytb	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	ACCCCTCCTCATATCCAACCA	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29032_Mgig_Bot2012_COI	PGI-B32	1	BD29032_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	TCTTATTCGTTGGAGACTTTATAACCCT	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29032_Mjap_Gib2022_COI	PGI-B32	1	BD29032_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	AATTTAATTGTGACTAGGCATGGTCTG	137.76542
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29033_Cmae_Bot2011_COI	PGI-B31	1	BD29033_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	ATGAACAGTCTATCCTCCTTTAG	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29033_Hsan_Wil2021_COI	PGI-B31	1	BD29033_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	CCTGGGCCGGTATAGTAGGT	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29033_Mros_Gib2022_COI	PGI-B31	1	BD29033_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29033_Msal_Bot2012_COI	PGI-B31	1	BD29033_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	GYTAGTTCCRATGATGTTAGCTG	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29033_Mstr_Wil2021_COI	PGI-B31	1	BD29033_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	GTAGTGGTAACTACTCATGCTTTA	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29033_Pvir_Dia2013_COI	PGI-B31	1	BD29033_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	CTTAGTGGCATTAATTCGDAATCC	137.76381
BD29033	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29033	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29033_Rhar_Sim2018_COI	PGI-B31	1	BD29033_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29033	Sample	BD29033	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B31	COI	45	BD29033	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B31	CTGCTGCTATTGCTCATG	137.76381
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29035_Aamu_Bax2006_COI	PGI-B29	1	BD29035_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	GCACAACCGGGATCTTTACTTC	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29035_Pcan_Bot2011_IGS	PGI-B29	1	BD29035_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B29	IGS	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	CGTAATCCTCAGTACTGGCTA	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29035_Pper_Dia2013_COI	PGI-B29	1	BD29035_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	CTTAGTGGCATTAATTCGDAATCC	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29035_Sspa_Oph2007_COI	PGI-B29	1	BD29035_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	GAAGGCCTTCCGATGATC	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29035_Upin_Bot2011_COI	PGI-B29	1	BD29035_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	CTTTAATTACAGCGTTTTTATTGTTGT	137.76983
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29036_Cjap_Sim2018_COI	PGI-B28	1	BD29036_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29036_Esin_And2018_Cyb	PGI-B28	1	BD29036_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B28	Cytb	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	ACCCCTCCTCATATCCAACCA	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29036_Mgig_Bot2012_COI	PGI-B28	1	BD29036_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	TCTTATTCGTTGGAGACTTTATAACCCT	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29036_Mjap_Gib2022_COI	PGI-B28	1	BD29036_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	AATTTAATTGTGACTAGGCATGGTCTG	137.76811
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29037_Cmae_Bot2011_COI	PGI-B27	1	BD29037_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	ATGAACAGTCTATCCTCCTTTAG	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29037_Hsan_Wil2021_COI	PGI-B27	1	BD29037_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	CCTGGGCCGGTATAGTAGGT	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29037_Mros_Gib2022_COI	PGI-B27	1	BD29037_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29037_Msal_Bot2012_COI	PGI-B27	1	BD29037_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	GYTAGTTCCRATGATGTTAGCTG	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29037_Mstr_Wil2021_COI	PGI-B27	1	BD29037_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	GTAGTGGTAACTACTCATGCTTTA	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29037_Pvir_Dia2013_COI	PGI-B27	1	BD29037_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	CTTAGTGGCATTAATTCGDAATCC	137.7663
BD29037	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01907	WGS84	BD29037	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29037_Rhar_Sim2018_COI	PGI-B27	1	BD29037_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29037	Sample	BD29037	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B27	COI	45	BD29037	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B27	CTGCTGCTATTGCTCATG	137.7663
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29039_Aamu_Bax2006_COI	PGI-B25	1	BD29039_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	GCACAACCGGGATCTTTACTTC	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29039_Pcan_Bot2011_IGS	PGI-B25	1	BD29039_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B25	IGS	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	CGTAATCCTCAGTACTGGCTA	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29039_Pper_Dia2013_COI	PGI-B25	1	BD29039_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	CTTAGTGGCATTAATTCGDAATCC	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29039_Sspa_Oph2007_COI	PGI-B25	1	BD29039_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	GAAGGCCTTCCGATGATC	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29039_Upin_Bot2011_COI	PGI-B25	1	BD29039_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	CTTTAATTACAGCGTTTTTATTGTTGT	137.77239
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29040_Cmae_Bot2011_COI	PGI-B24	1	BD29040_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	ATGAACAGTCTATCCTCCTTTAG	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29040_Hsan_Wil2021_COI	PGI-B24	1	BD29040_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	CCTGGGCCGGTATAGTAGGT	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29040_Mros_Gib2022_COI	PGI-B24	1	BD29040_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29040_Msal_Bot2012_COI	PGI-B24	1	BD29040_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	GYTAGTTCCRATGATGTTAGCTG	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29040_Mstr_Wil2021_COI	PGI-B24	1	BD29040_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	GTAGTGGTAACTACTCATGCTTTA	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29040_Pvir_Dia2013_COI	PGI-B24	1	BD29040_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	CTTAGTGGCATTAATTCGDAATCC	137.77071
BD29040	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02023	WGS84	BD29040	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29040_Rhar_Sim2018_COI	PGI-B24	1	BD29040_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29040	Sample	BD29040	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B24	COI	45	BD29040	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B24	CTGCTGCTATTGCTCATG	137.77071
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29042_Aamu_Bax2006_COI	PGI-B22	1	BD29042_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	GCACAACCGGGATCTTTACTTC	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29042_Pcan_Bot2011_IGS	PGI-B22	1	BD29042_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B22	IGS	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	CGTAATCCTCAGTACTGGCTA	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29042_Pper_Dia2013_COI	PGI-B22	1	BD29042_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	CTTAGTGGCATTAATTCGDAATCC	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29042_Sspa_Oph2007_COI	PGI-B22	1	BD29042_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	GAAGGCCTTCCGATGATC	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29042_Upin_Bot2011_COI	PGI-B22	1	BD29042_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	CTTTAATTACAGCGTTTTTATTGTTGT	137.76718
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29043_Cjap_Sim2018_COI	PGI-B21	1	BD29043_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29043_Esin_And2018_Cyb	PGI-B21	1	BD29043_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B21	Cytb	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	ACCCCTCCTCATATCCAACCA	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29043_Mgig_Bot2012_COI	PGI-B21	1	BD29043_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	TCTTATTCGTTGGAGACTTTATAACCCT	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29043_Mjap_Gib2022_COI	PGI-B21	1	BD29043_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	AATTTAATTGTGACTAGGCATGGTCTG	137.76543
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29044_Cmae_Bot2011_COI	PGI-B20	1	BD29044_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	ATGAACAGTCTATCCTCCTTTAG	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29044_Hsan_Wil2021_COI	PGI-B20	1	BD29044_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	CCTGGGCCGGTATAGTAGGT	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29044_Mros_Gib2022_COI	PGI-B20	1	BD29044_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29044_Msal_Bot2012_COI	PGI-B20	1	BD29044_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	GYTAGTTCCRATGATGTTAGCTG	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29044_Mstr_Wil2021_COI	PGI-B20	1	BD29044_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	GTAGTGGTAACTACTCATGCTTTA	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29044_Pvir_Dia2013_COI	PGI-B20	1	BD29044_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	CTTAGTGGCATTAATTCGDAATCC	137.76397
BD29044	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02024	WGS84	BD29044	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29044_Rhar_Sim2018_COI	PGI-B20	1	BD29044_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29044	Sample	BD29044	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B20	COI	45	BD29044	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B20	CTGCTGCTATTGCTCATG	137.76397
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29046_Aamu_Bax2006_COI	PGI-B18	1	BD29046_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	GCACAACCGGGATCTTTACTTC	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29046_Pcan_Bot2011_IGS	PGI-B18	1	BD29046_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B18	IGS	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	CGTAATCCTCAGTACTGGCTA	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29046_Pper_Dia2013_COI	PGI-B18	1	BD29046_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	CTTAGTGGCATTAATTCGDAATCC	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29046_Sspa_Oph2007_COI	PGI-B18	1	BD29046_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	GAAGGCCTTCCGATGATC	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29046_Upin_Bot2011_COI	PGI-B18	1	BD29046_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	CTTTAATTACAGCGTTTTTATTGTTGT	137.77165
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29047_Cjap_Sim2018_COI	PGI-B17	1	BD29047_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29047_Esin_And2018_Cyb	PGI-B17	1	BD29047_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B17	Cytb	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	ACCCCTCCTCATATCCAACCA	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29047_Mgig_Bot2012_COI	PGI-B17	1	BD29047_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	TCTTATTCGTTGGAGACTTTATAACCCT	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29047_Mjap_Gib2022_COI	PGI-B17	1	BD29047_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	AATTTAATTGTGACTAGGCATGGTCTG	137.76988
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29048_Cmae_Bot2011_COI	PGI-B16	1	BD29048_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	ATGAACAGTCTATCCTCCTTTAG	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29048_Hsan_Wil2021_COI	PGI-B16	1	BD29048_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	CCTGGGCCGGTATAGTAGGT	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29048_Mros_Gib2022_COI	PGI-B16	1	BD29048_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29048_Msal_Bot2012_COI	PGI-B16	1	BD29048_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	GYTAGTTCCRATGATGTTAGCTG	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29048_Mstr_Wil2021_COI	PGI-B16	1	BD29048_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	GTAGTGGTAACTACTCATGCTTTA	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29048_Pvir_Dia2013_COI	PGI-B16	1	BD29048_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	CTTAGTGGCATTAATTCGDAATCC	137.77252
BD29048	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02257	WGS84	BD29048	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29048_Rhar_Sim2018_COI	PGI-B16	1	BD29048_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29048	Sample	BD29048	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B16	COI	45	BD29048	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B16	CTGCTGCTATTGCTCATG	137.77252
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29050_Cjap_Sim2018_COI	PGI-B14	1	BD29050_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29050_Esin_And2018_Cyb	PGI-B14	1	BD29050_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B14	Cytb	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	ACCCCTCCTCATATCCAACCA	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29050_Mgig_Bot2012_COI	PGI-B14	1	BD29050_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	TCTTATTCGTTGGAGACTTTATAACCCT	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29050_Mjap_Gib2022_COI	PGI-B14	1	BD29050_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	AATTTAATTGTGACTAGGCATGGTCTG	137.76906
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29051_Cmae_Bot2011_COI	PGI-B13	1	BD29051_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	ATGAACAGTCTATCCTCCTTTAG	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29051_Hsan_Wil2021_COI	PGI-B13	1	BD29051_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	CCTGGGCCGGTATAGTAGGT	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29051_Mros_Gib2022_COI	PGI-B13	1	BD29051_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29051_Msal_Bot2012_COI	PGI-B13	1	BD29051_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	GYTAGTTCCRATGATGTTAGCTG	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29051_Mstr_Wil2021_COI	PGI-B13	1	BD29051_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	GTAGTGGTAACTACTCATGCTTTA	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29051_Pvir_Dia2013_COI	PGI-B13	1	BD29051_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	CTTAGTGGCATTAATTCGDAATCC	137.7673
BD29051	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02272	WGS84	BD29051	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29051_Rhar_Sim2018_COI	PGI-B13	1	BD29051_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29051	Sample	BD29051	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B13	COI	45	BD29051	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B13	CTGCTGCTATTGCTCATG	137.7673
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29053_Aamu_Bax2006_COI	PGI-B11	1	BD29053_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	GCACAACCGGGATCTTTACTTC	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29053_Pcan_Bot2011_IGS	PGI-B11	1	BD29053_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B11	IGS	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	CGTAATCCTCAGTACTGGCTA	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29053_Pper_Dia2013_COI	PGI-B11	1	BD29053_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	CTTAGTGGCATTAATTCGDAATCC	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29053_Sspa_Oph2007_COI	PGI-B11	1	BD29053_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	GAAGGCCTTCCGATGATC	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29053_Upin_Bot2011_COI	PGI-B11	1	BD29053_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	CTTTAATTACAGCGTTTTTATTGTTGT	137.76396
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29054_Cjap_Sim2018_COI	PGI-B10	1	BD29054_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29054_Esin_And2018_Cyb	PGI-B10	1	BD29054_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B10	Cytb	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	ACCCCTCCTCATATCCAACCA	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29054_Mgig_Bot2012_COI	PGI-B10	1	BD29054_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	TCTTATTCGTTGGAGACTTTATAACCCT	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29054_Mjap_Gib2022_COI	PGI-B10	1	BD29054_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	AATTTAATTGTGACTAGGCATGGTCTG	137.77167
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29055_Cmae_Bot2011_COI	PGI-B09	1	BD29055_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	ATGAACAGTCTATCCTCCTTTAG	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29055_Hsan_Wil2021_COI	PGI-B09	1	BD29055_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	CCTGGGCCGGTATAGTAGGT	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29055_Mros_Gib2022_COI	PGI-B09	1	BD29055_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29055_Msal_Bot2012_COI	PGI-B09	1	BD29055_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	GYTAGTTCCRATGATGTTAGCTG	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29055_Mstr_Wil2021_COI	PGI-B09	1	BD29055_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	GTAGTGGTAACTACTCATGCTTTA	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29055_Pvir_Dia2013_COI	PGI-B09	1	BD29055_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	CTTAGTGGCATTAATTCGDAATCC	137.76986
BD29055	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29055	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29055_Rhar_Sim2018_COI	PGI-B09	1	BD29055_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29055	Sample	BD29055	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B09	COI	45	BD29055	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B09	CTGCTGCTATTGCTCATG	137.76986
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29057_Aamu_Bax2006_COI	PGI-B07	1	BD29057_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	GCACAACCGGGATCTTTACTTC	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29057_Pcan_Bot2011_IGS	PGI-B07	1	BD29057_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B07	IGS	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	CGTAATCCTCAGTACTGGCTA	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29057_Pper_Dia2013_COI	PGI-B07	1	BD29057_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	CTTAGTGGCATTAATTCGDAATCC	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29057_Sspa_Oph2007_COI	PGI-B07	1	BD29057_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	GAAGGCCTTCCGATGATC	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29057_Upin_Bot2011_COI	PGI-B07	1	BD29057_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	CTTTAATTACAGCGTTTTTATTGTTGT	137.76643
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29058_Cjap_Sim2018_COI	PGI-B06	1	BD29058_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29058_Esin_And2018_Cyb	PGI-B06	1	BD29058_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B06	Cytb	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	ACCCCTCCTCATATCCAACCA	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29058_Mgig_Bot2012_COI	PGI-B06	1	BD29058_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	TCTTATTCGTTGGAGACTTTATAACCCT	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29058_Mjap_Gib2022_COI	PGI-B06	1	BD29058_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	AATTTAATTGTGACTAGGCATGGTCTG	137.76476
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29059_Cmae_Bot2011_COI	PGI-B05	1	BD29059_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	ATGAACAGTCTATCCTCCTTTAG	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29059_Hsan_Wil2021_COI	PGI-B05	1	BD29059_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	CCTGGGCCGGTATAGTAGGT	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29059_Mros_Gib2022_COI	PGI-B05	1	BD29059_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29059_Msal_Bot2012_COI	PGI-B05	1	BD29059_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	GYTAGTTCCRATGATGTTAGCTG	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29059_Mstr_Wil2021_COI	PGI-B05	1	BD29059_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	GTAGTGGTAACTACTCATGCTTTA	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29059_Pvir_Dia2013_COI	PGI-B05	1	BD29059_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	CTTAGTGGCATTAATTCGDAATCC	137.77076
BD29059	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0253	WGS84	BD29059	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29059_Rhar_Sim2018_COI	PGI-B05	1	BD29059_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29059	Sample	BD29059	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B05	COI	45	BD29059	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B05	CTGCTGCTATTGCTCATG	137.77076
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29060_Aamu_Bax2006_COI	PGI-B04	1	BD29060_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	GCACAACCGGGATCTTTACTTC	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29060_Pcan_Bot2011_IGS	PGI-B04	1	BD29060_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B04	IGS	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	CGTAATCCTCAGTACTGGCTA	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29060_Pper_Dia2013_COI	PGI-B04	1	BD29060_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	CTTAGTGGCATTAATTCGDAATCC	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29060_Sspa_Oph2007_COI	PGI-B04	1	BD29060_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	GAAGGCCTTCCGATGATC	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29060_Upin_Bot2011_COI	PGI-B04	1	BD29060_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	CTTTAATTACAGCGTTTTTATTGTTGT	137.76916
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29061_Cjap_Sim2018_COI	PGI-B03	1	BD29061_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29061_Esin_And2018_Cyb	PGI-B03	1	BD29061_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B03	Cytb	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	ACCCCTCCTCATATCCAACCA	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29061_Mgig_Bot2012_COI	PGI-B03	1	BD29061_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	TCTTATTCGTTGGAGACTTTATAACCCT	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29061_Mjap_Gib2022_COI	PGI-B03	1	BD29061_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	AATTTAATTGTGACTAGGCATGGTCTG	137.76729
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29062_Cmae_Bot2011_COI	PGI-B02	1	BD29062_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	ATGAACAGTCTATCCTCCTTTAG	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29062_Hsan_Wil2021_COI	PGI-B02	1	BD29062_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	CCTGGGCCGGTATAGTAGGT	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29062_Mros_Gib2022_COI	PGI-B02	1	BD29062_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29062_Msal_Bot2012_COI	PGI-B02	1	BD29062_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	GYTAGTTCCRATGATGTTAGCTG	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29062_Mstr_Wil2021_COI	PGI-B02	1	BD29062_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	GTAGTGGTAACTACTCATGCTTTA	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29062_Pvir_Dia2013_COI	PGI-B02	1	BD29062_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	CTTAGTGGCATTAATTCGDAATCC	137.76554
BD29062	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02523	WGS84	BD29062	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29062_Rhar_Sim2018_COI	PGI-B02	1	BD29062_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29062	Sample	BD29062	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B02	COI	45	BD29062	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B02	CTGCTGCTATTGCTCATG	137.76554
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29064_Aamu_Bax2006_COI	KPT-B35	1	BD29064_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	GCACAACCGGGATCTTTACTTC	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29064_Pcan_Bot2011_IGS	KPT-B35	1	BD29064_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B35	IGS	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	CGTAATCCTCAGTACTGGCTA	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29064_Pper_Dia2013_COI	KPT-B35	1	BD29064_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	CTTAGTGGCATTAATTCGDAATCC	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29064_Sspa_Oph2007_COI	KPT-B35	1	BD29064_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	GAAGGCCTTCCGATGATC	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29064_Upin_Bot2011_COI	KPT-B35	1	BD29064_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	CTTTAATTACAGCGTTTTTATTGTTGT	137.78064
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29065_Cjap_Sim2018_COI	KPT-B34	1	BD29065_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29065_Esin_And2018_Cyb	KPT-B34	1	BD29065_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B34	Cytb	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	ACCCCTCCTCATATCCAACCA	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29065_Mgig_Bot2012_COI	KPT-B34	1	BD29065_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	TCTTATTCGTTGGAGACTTTATAACCCT	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29065_Mjap_Gib2022_COI	KPT-B34	1	BD29065_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	AATTTAATTGTGACTAGGCATGGTCTG	137.77922
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29066_Cmae_Bot2011_COI	KPT-B33	1	BD29066_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	ATGAACAGTCTATCCTCCTTTAG	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29066_Hsan_Wil2021_COI	KPT-B33	1	BD29066_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	CCTGGGCCGGTATAGTAGGT	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29066_Mros_Gib2022_COI	KPT-B33	1	BD29066_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29066_Msal_Bot2012_COI	KPT-B33	1	BD29066_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	GYTAGTTCCRATGATGTTAGCTG	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29066_Mstr_Wil2021_COI	KPT-B33	1	BD29066_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	GTAGTGGTAACTACTCATGCTTTA	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29066_Pvir_Dia2013_COI	KPT-B33	1	BD29066_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	CTTAGTGGCATTAATTCGDAATCC	137.77795
BD29066	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96135	WGS84	BD29066	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29066_Rhar_Sim2018_COI	KPT-B33	1	BD29066_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29066	Sample	BD29066	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B33	COI	45	BD29066	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B33	CTGCTGCTATTGCTCATG	137.77795
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29068_Aamu_Bax2006_COI	KPT-B31	1	BD29068_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	GCACAACCGGGATCTTTACTTC	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29068_Pcan_Bot2011_IGS	KPT-B31	1	BD29068_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B31	IGS	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	CGTAATCCTCAGTACTGGCTA	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29068_Pper_Dia2013_COI	KPT-B31	1	BD29068_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	CTTAGTGGCATTAATTCGDAATCC	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29068_Sspa_Oph2007_COI	KPT-B31	1	BD29068_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	GAAGGCCTTCCGATGATC	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29068_Upin_Bot2011_COI	KPT-B31	1	BD29068_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	CTTTAATTACAGCGTTTTTATTGTTGT	137.77987
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29069_Cjap_Sim2018_COI	KPT-B30	1	BD29069_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29069_Esin_And2018_Cyb	KPT-B30	1	BD29069_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B30	Cytb	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	ACCCCTCCTCATATCCAACCA	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29069_Mgig_Bot2012_COI	KPT-B30	1	BD29069_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	TCTTATTCGTTGGAGACTTTATAACCCT	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29069_Mjap_Gib2022_COI	KPT-B30	1	BD29069_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	AATTTAATTGTGACTAGGCATGGTCTG	137.77857
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29071_Aamu_Bax2006_COI	KPT-B28	1	BD29071_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	GCACAACCGGGATCTTTACTTC	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29071_Pcan_Bot2011_IGS	KPT-B28	1	BD29071_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B28	IGS	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	CGTAATCCTCAGTACTGGCTA	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29071_Pper_Dia2013_COI	KPT-B28	1	BD29071_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	CTTAGTGGCATTAATTCGDAATCC	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29071_Upin_Bot2011_COI	KPT-B28	1	BD29071_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	CTTTAATTACAGCGTTTTTATTGTTGT	137.77599
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29072_Cjap_Sim2018_COI	KPT-B27	1	BD29072_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29072_Esin_And2018_Cyb	KPT-B27	1	BD29072_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B27	Cytb	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	ACCCCTCCTCATATCCAACCA	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29072_Mgig_Bot2012_COI	KPT-B27	1	BD29072_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	TCTTATTCGTTGGAGACTTTATAACCCT	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29072_Mjap_Gib2022_COI	KPT-B27	1	BD29072_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	AATTTAATTGTGACTAGGCATGGTCTG	137.77929
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29073_Cmae_Bot2011_COI	KPT-B26	1	BD29073_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	ATGAACAGTCTATCCTCCTTTAG	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29073_Hsan_Wil2021_COI	KPT-B26	1	BD29073_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	CCTGGGCCGGTATAGTAGGT	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29073_Mros_Gib2022_COI	KPT-B26	1	BD29073_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29073_Msal_Bot2012_COI	KPT-B26	1	BD29073_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	GYTAGTTCCRATGATGTTAGCTG	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29073_Mstr_Wil2021_COI	KPT-B26	1	BD29073_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	GTAGTGGTAACTACTCATGCTTTA	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29073_Pvir_Dia2013_COI	KPT-B26	1	BD29073_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	CTTAGTGGCATTAATTCGDAATCC	137.77788
BD29073	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95951	WGS84	BD29073	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29073_Rhar_Sim2018_COI	KPT-B26	1	BD29073_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29073	Sample	BD29073	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B26	COI	45	BD29073	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B26	CTGCTGCTATTGCTCATG	137.77788
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29075_Aamu_Bax2006_COI	KPT-B24	1	BD29075_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	GCACAACCGGGATCTTTACTTC	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29075_Pcan_Bot2011_IGS	KPT-B24	1	BD29075_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B24	IGS	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	CGTAATCCTCAGTACTGGCTA	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29075_Pper_Dia2013_COI	KPT-B24	1	BD29075_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	CTTAGTGGCATTAATTCGDAATCC	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29075_Sspa_Oph2007_COI	KPT-B24	1	BD29075_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	GAAGGCCTTCCGATGATC	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29075_Upin_Bot2011_COI	KPT-B24	1	BD29075_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	CTTTAATTACAGCGTTTTTATTGTTGT	137.77851
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29076_Cjap_Sim2018_COI	KPT-B23	1	BD29076_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29076_Esin_And2018_Cyb	KPT-B23	1	BD29076_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B23	Cytb	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	ACCCCTCCTCATATCCAACCA	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29076_Mgig_Bot2012_COI	KPT-B23	1	BD29076_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	TCTTATTCGTTGGAGACTTTATAACCCT	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29076_Mjap_Gib2022_COI	KPT-B23	1	BD29076_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	AATTTAATTGTGACTAGGCATGGTCTG	137.77717
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29077_Cmae_Bot2011_COI	KPT-B22	1	BD29077_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	ATGAACAGTCTATCCTCCTTTAG	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29077_Hsan_Wil2021_COI	KPT-B22	1	BD29077_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	CCTGGGCCGGTATAGTAGGT	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29077_Mros_Gib2022_COI	KPT-B22	1	BD29077_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29077_Msal_Bot2012_COI	KPT-B22	1	BD29077_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	GYTAGTTCCRATGATGTTAGCTG	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29077_Mstr_Wil2021_COI	KPT-B22	1	BD29077_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	GTAGTGGTAACTACTCATGCTTTA	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29077_Pvir_Dia2013_COI	KPT-B22	1	BD29077_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	CTTAGTGGCATTAATTCGDAATCC	137.77776
BD29077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95769	WGS84	BD29077	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29077_Rhar_Sim2018_COI	KPT-B22	1	BD29077_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29077	Sample	BD29077	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B22	COI	45	BD29077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B22	CTGCTGCTATTGCTCATG	137.77776
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29079_Aamu_Bax2006_COI	KPT-B20	1	BD29079_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	GCACAACCGGGATCTTTACTTC	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29079_Pcan_Bot2011_IGS	KPT-B20	1	BD29079_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B20	IGS	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	CGTAATCCTCAGTACTGGCTA	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29079_Pper_Dia2013_COI	KPT-B20	1	BD29079_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	CTTAGTGGCATTAATTCGDAATCC	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29079_Sspa_Oph2007_COI	KPT-B20	1	BD29079_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	GAAGGCCTTCCGATGATC	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29079_Upin_Bot2011_COI	KPT-B20	1	BD29079_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	CTTTAATTACAGCGTTTTTATTGTTGT	137.77984
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29080_Cmae_Bot2011_COI	KPT-B19	1	BD29080_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	ATGAACAGTCTATCCTCCTTTAG	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29080_Hsan_Wil2021_COI	KPT-B19	1	BD29080_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	CCTGGGCCGGTATAGTAGGT	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29080_Mros_Gib2022_COI	KPT-B19	1	BD29080_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29080_Msal_Bot2012_COI	KPT-B19	1	BD29080_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	GYTAGTTCCRATGATGTTAGCTG	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29080_Mstr_Wil2021_COI	KPT-B19	1	BD29080_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	GTAGTGGTAACTACTCATGCTTTA	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29080_Pvir_Dia2013_COI	KPT-B19	1	BD29080_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	CTTAGTGGCATTAATTCGDAATCC	137.77857
BD29080	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.9622	WGS84	BD29080	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29080_Rhar_Sim2018_COI	KPT-B19	1	BD29080_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29080	Sample	BD29080	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B19	COI	45	BD29080	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B19	CTGCTGCTATTGCTCATG	137.77857
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29082_Aamu_Bax2006_COI	KPT-B17	1	BD29082_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	GCACAACCGGGATCTTTACTTC	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29082_Pcan_Bot2011_IGS	KPT-B17	1	BD29082_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B17	IGS	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	CGTAATCCTCAGTACTGGCTA	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29082_Pper_Dia2013_COI	KPT-B17	1	BD29082_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	CTTAGTGGCATTAATTCGDAATCC	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29082_Sspa_Oph2007_COI	KPT-B17	1	BD29082_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	GAAGGCCTTCCGATGATC	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29082_Upin_Bot2011_COI	KPT-B17	1	BD29082_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	CTTTAATTACAGCGTTTTTATTGTTGT	137.77611
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29083_Cjap_Sim2018_COI	KPT-B16	1	BD29083_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29083_Esin_And2018_Cyb	KPT-B16	1	BD29083_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B16	Cytb	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	ACCCCTCCTCATATCCAACCA	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29083_Mgig_Bot2012_COI	KPT-B16	1	BD29083_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	TCTTATTCGTTGGAGACTTTATAACCCT	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29083_Mjap_Gib2022_COI	KPT-B16	1	BD29083_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	AATTTAATTGTGACTAGGCATGGTCTG	137.78061
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29084_Cmae_Bot2011_COI	KPT-B15	1	BD29084_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	ATGAACAGTCTATCCTCCTTTAG	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29084_Hsan_Wil2021_COI	KPT-B15	1	BD29084_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	CCTGGGCCGGTATAGTAGGT	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29084_Mros_Gib2022_COI	KPT-B15	1	BD29084_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29084_Msal_Bot2012_COI	KPT-B15	1	BD29084_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	GYTAGTTCCRATGATGTTAGCTG	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29084_Mstr_Wil2021_COI	KPT-B15	1	BD29084_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	GTAGTGGTAACTACTCATGCTTTA	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29084_Pvir_Dia2013_COI	KPT-B15	1	BD29084_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	CTTAGTGGCATTAATTCGDAATCC	137.77929
BD29084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96306	WGS84	BD29084	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29084_Rhar_Sim2018_COI	KPT-B15	1	BD29084_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29084	Sample	BD29084	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B15	COI	45	BD29084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B15	CTGCTGCTATTGCTCATG	137.77929
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29086_Aamu_Bax2006_COI	KPT-B13	1	BD29086_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	GCACAACCGGGATCTTTACTTC	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29086_Pcan_Bot2011_IGS	KPT-B13	1	BD29086_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B13	IGS	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	CGTAATCCTCAGTACTGGCTA	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29086_Pper_Dia2013_COI	KPT-B13	1	BD29086_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	CTTAGTGGCATTAATTCGDAATCC	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29086_Sspa_Oph2007_COI	KPT-B13	1	BD29086_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	GAAGGCCTTCCGATGATC	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29086_Upin_Bot2011_COI	KPT-B13	1	BD29086_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	CTTTAATTACAGCGTTTTTATTGTTGT	137.77686
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29087_Cjap_Sim2018_COI	KPT-B12	1	BD29087_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29087_Esin_And2018_Cyb	KPT-B12	1	BD29087_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B12	Cytb	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	ACCCCTCCTCATATCCAACCA	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29087_Mgig_Bot2012_COI	KPT-B12	1	BD29087_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	TCTTATTCGTTGGAGACTTTATAACCCT	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29087_Mjap_Gib2022_COI	KPT-B12	1	BD29087_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	AATTTAATTGTGACTAGGCATGGTCTG	137.7754
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29088_Cmae_Bot2011_COI	KPT-B11	1	BD29088_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	ATGAACAGTCTATCCTCCTTTAG	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29088_Hsan_Wil2021_COI	KPT-B11	1	BD29088_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	CCTGGGCCGGTATAGTAGGT	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29088_Mros_Gib2022_COI	KPT-B11	1	BD29088_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29088_Msal_Bot2012_COI	KPT-B11	1	BD29088_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	GYTAGTTCCRATGATGTTAGCTG	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29088_Mstr_Wil2021_COI	KPT-B11	1	BD29088_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	GTAGTGGTAACTACTCATGCTTTA	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29088_Pvir_Dia2013_COI	KPT-B11	1	BD29088_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	CTTAGTGGCATTAATTCGDAATCC	137.77407
BD29088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96342	WGS84	BD29088	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29088_Rhar_Sim2018_COI	KPT-B11	1	BD29088_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29088	Sample	BD29088	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B11	COI	45	BD29088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B11	CTGCTGCTATTGCTCATG	137.77407
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29090_Cjap_Sim2018_COI	KPT-B09	1	BD29090_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29090_Esin_And2018_Cyb	KPT-B09	1	BD29090_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B09	Cytb	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	ACCCCTCCTCATATCCAACCA	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29090_Mgig_Bot2012_COI	KPT-B09	1	BD29090_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	TCTTATTCGTTGGAGACTTTATAACCCT	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29090_Mjap_Gib2022_COI	KPT-B09	1	BD29090_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	AATTTAATTGTGACTAGGCATGGTCTG	137.77871
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29091_Cmae_Bot2011_COI	KPT-B08	1	BD29091_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	ATGAACAGTCTATCCTCCTTTAG	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29091_Hsan_Wil2021_COI	KPT-B08	1	BD29091_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	CCTGGGCCGGTATAGTAGGT	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29091_Mros_Gib2022_COI	KPT-B08	1	BD29091_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29091_Msal_Bot2012_COI	KPT-B08	1	BD29091_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	GYTAGTTCCRATGATGTTAGCTG	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29091_Mstr_Wil2021_COI	KPT-B08	1	BD29091_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	GTAGTGGTAACTACTCATGCTTTA	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29091_Pvir_Dia2013_COI	KPT-B08	1	BD29091_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	CTTAGTGGCATTAATTCGDAATCC	137.77739
BD29091	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29091	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29091_Rhar_Sim2018_COI	KPT-B08	1	BD29091_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29091	Sample	BD29091	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B08	COI	45	BD29091	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B08	CTGCTGCTATTGCTCATG	137.77739
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29093_Aamu_Bax2006_COI	KPT-B06	1	BD29093_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	GCACAACCGGGATCTTTACTTC	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29093_Pcan_Bot2011_IGS	KPT-B06	1	BD29093_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B06	IGS	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	CGTAATCCTCAGTACTGGCTA	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29093_Pper_Dia2013_COI	KPT-B06	1	BD29093_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	CTTAGTGGCATTAATTCGDAATCC	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29093_Sspa_Oph2007_COI	KPT-B06	1	BD29093_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	GAAGGCCTTCCGATGATC	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29093_Upin_Bot2011_COI	KPT-B06	1	BD29093_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	CTTTAATTACAGCGTTTTTATTGTTGT	137.77479
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29094_Cjap_Sim2018_COI	KPT-B05	1	BD29094_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29094_Esin_And2018_Cyb	KPT-B05	1	BD29094_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B05	Cytb	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	ACCCCTCCTCATATCCAACCA	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29094_Mgig_Bot2012_COI	KPT-B05	1	BD29094_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	TCTTATTCGTTGGAGACTTTATAACCCT	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29094_Mjap_Gib2022_COI	KPT-B05	1	BD29094_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	AATTTAATTGTGACTAGGCATGGTCTG	137.77363
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29095_Cmae_Bot2011_COI	KPT-B04	1	BD29095_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	ATGAACAGTCTATCCTCCTTTAG	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29095_Hsan_Wil2021_COI	KPT-B04	1	BD29095_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	CCTGGGCCGGTATAGTAGGT	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29095_Mros_Gib2022_COI	KPT-B04	1	BD29095_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29095_Msal_Bot2012_COI	KPT-B04	1	BD29095_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	GYTAGTTCCRATGATGTTAGCTG	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29095_Mstr_Wil2021_COI	KPT-B04	1	BD29095_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	GTAGTGGTAACTACTCATGCTTTA	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29095_Pvir_Dia2013_COI	KPT-B04	1	BD29095_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	CTTAGTGGCATTAATTCGDAATCC	137.77813
BD29095	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29095	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29095_Rhar_Sim2018_COI	KPT-B04	1	BD29095_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29095	Sample	BD29095	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B04	COI	45	BD29095	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B04	CTGCTGCTATTGCTCATG	137.77813
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29097_Aamu_Bax2006_COI	KPT-B02	1	BD29097_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	GCACAACCGGGATCTTTACTTC	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29097_Pcan_Bot2011_IGS	KPT-B02	1	BD29097_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B02	IGS	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	CGTAATCCTCAGTACTGGCTA	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29097_Pper_Dia2013_COI	KPT-B02	1	BD29097_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	CTTAGTGGCATTAATTCGDAATCC	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29097_Sspa_Oph2007_COI	KPT-B02	1	BD29097_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	GAAGGCCTTCCGATGATC	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29097_Upin_Bot2011_COI	KPT-B02	1	BD29097_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	CTTTAATTACAGCGTTTTTATTGTTGT	137.77554
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29098_Cjap_Sim2018_COI	KPT-B01	1	BD29098_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29098_Esin_And2018_Cyb	KPT-B01	1	BD29098_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B01	Cytb	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	ACCCCTCCTCATATCCAACCA	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29098_Mgig_Bot2012_COI	KPT-B01	1	BD29098_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	TCTTATTCGTTGGAGACTTTATAACCCT	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29098_Mjap_Gib2022_COI	KPT-B01	1	BD29098_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	AATTTAATTGTGACTAGGCATGGTCTG	137.77442
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29099_Cmae_Bot2011_COI	PLO-B35	1	BD29099_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	ATGAACAGTCTATCCTCCTTTAG	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29099_Hsan_Wil2021_COI	PLO-B35	1	BD29099_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	CCTGGGCCGGTATAGTAGGT	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29099_Mros_Gib2022_COI	PLO-B35	1	BD29099_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29099_Msal_Bot2012_COI	PLO-B35	1	BD29099_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	GYTAGTTCCRATGATGTTAGCTG	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29099_Mstr_Wil2021_COI	PLO-B35	1	BD29099_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	GTAGTGGTAACTACTCATGCTTTA	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29099_Pvir_Dia2013_COI	PLO-B35	1	BD29099_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	CTTAGTGGCATTAATTCGDAATCC	135.87716
BD29099	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71941	WGS84	BD29099	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29099_Rhar_Sim2018_COI	PLO-B35	1	BD29099_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29099	Sample	BD29099	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B35	COI	45	BD29099	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B35	CTGCTGCTATTGCTCATG	135.87716
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29101_Aamu_Bax2006_COI	PLO-B33	1	BD29101_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	GCACAACCGGGATCTTTACTTC	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29101_Pcan_Bot2011_IGS	PLO-B33	1	BD29101_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B33	IGS	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	CGTAATCCTCAGTACTGGCTA	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29101_Pper_Dia2013_COI	PLO-B33	1	BD29101_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	CTTAGTGGCATTAATTCGDAATCC	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29101_Sspa_Oph2007_COI	PLO-B33	1	BD29101_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	GAAGGCCTTCCGATGATC	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29101_Upin_Bot2011_COI	PLO-B33	1	BD29101_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	CTTTAATTACAGCGTTTTTATTGTTGT	135.87046
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29102_Cjap_Sim2018_COI	PLO-B32	1	BD29102_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29102_Esin_And2018_Cyb	PLO-B32	1	BD29102_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B32	Cytb	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	ACCCCTCCTCATATCCAACCA	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29102_Mgig_Bot2012_COI	PLO-B32	1	BD29102_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	TCTTATTCGTTGGAGACTTTATAACCCT	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29102_Mjap_Gib2022_COI	PLO-B32	1	BD29102_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	AATTTAATTGTGACTAGGCATGGTCTG	135.86825
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29103_Cmae_Bot2011_COI	PLO-B31	1	BD29103_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	ATGAACAGTCTATCCTCCTTTAG	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29103_Hsan_Wil2021_COI	PLO-B31	1	BD29103_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	CCTGGGCCGGTATAGTAGGT	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29103_Mros_Gib2022_COI	PLO-B31	1	BD29103_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29103_Msal_Bot2012_COI	PLO-B31	1	BD29103_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	GYTAGTTCCRATGATGTTAGCTG	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29103_Mstr_Wil2021_COI	PLO-B31	1	BD29103_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	GTAGTGGTAACTACTCATGCTTTA	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29103_Pvir_Dia2013_COI	PLO-B31	1	BD29103_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	CTTAGTGGCATTAATTCGDAATCC	135.86615
BD29103	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71316	WGS84	BD29103	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29103_Rhar_Sim2018_COI	PLO-B31	1	BD29103_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29103	Sample	BD29103	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B31	COI	45	BD29103	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B31	CTGCTGCTATTGCTCATG	135.86615
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29105_Aamu_Bax2006_COI	PLO-B29	1	BD29105_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	GCACAACCGGGATCTTTACTTC	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29105_Pcan_Bot2011_IGS	PLO-B29	1	BD29105_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B29	IGS	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	CGTAATCCTCAGTACTGGCTA	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29105_Pper_Dia2013_COI	PLO-B29	1	BD29105_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	CTTAGTGGCATTAATTCGDAATCC	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29105_Sspa_Oph2007_COI	PLO-B29	1	BD29105_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	GAAGGCCTTCCGATGATC	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29105_Upin_Bot2011_COI	PLO-B29	1	BD29105_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	CTTTAATTACAGCGTTTTTATTGTTGT	135.86743
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29106_Cjap_Sim2018_COI	PLO-B28	1	BD29106_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29106_Esin_And2018_Cyb	PLO-B28	1	BD29106_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B28	Cytb	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	ACCCCTCCTCATATCCAACCA	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29106_Mgig_Bot2012_COI	PLO-B28	1	BD29106_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	TCTTATTCGTTGGAGACTTTATAACCCT	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29106_Mjap_Gib2022_COI	PLO-B28	1	BD29106_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	AATTTAATTGTGACTAGGCATGGTCTG	135.86504
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29107_Cmae_Bot2011_COI	PLO-B27	1	BD29107_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	ATGAACAGTCTATCCTCCTTTAG	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29107_Hsan_Wil2021_COI	PLO-B27	1	BD29107_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	CCTGGGCCGGTATAGTAGGT	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29107_Mros_Gib2022_COI	PLO-B27	1	BD29107_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29107_Msal_Bot2012_COI	PLO-B27	1	BD29107_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	GYTAGTTCCRATGATGTTAGCTG	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29107_Mstr_Wil2021_COI	PLO-B27	1	BD29107_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	GTAGTGGTAACTACTCATGCTTTA	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29107_Pvir_Dia2013_COI	PLO-B27	1	BD29107_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	CTTAGTGGCATTAATTCGDAATCC	135.86401
BD29107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71641	WGS84	BD29107	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29107_Rhar_Sim2018_COI	PLO-B27	1	BD29107_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29107	Sample	BD29107	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B27	COI	45	BD29107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B27	CTGCTGCTATTGCTCATG	135.86401
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29109_Aamu_Bax2006_COI	PLO-B25	1	BD29109_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	GCACAACCGGGATCTTTACTTC	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29109_Pcan_Bot2011_IGS	PLO-B25	1	BD29109_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B25	IGS	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	CGTAATCCTCAGTACTGGCTA	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29109_Pper_Dia2013_COI	PLO-B25	1	BD29109_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	CTTAGTGGCATTAATTCGDAATCC	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29109_Sspa_Oph2007_COI	PLO-B25	1	BD29109_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	GAAGGCCTTCCGATGATC	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29109_Upin_Bot2011_COI	PLO-B25	1	BD29109_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	CTTTAATTACAGCGTTTTTATTGTTGT	135.86842
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29110_Cmae_Bot2011_COI	PLO-B24	1	BD29110_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	ATGAACAGTCTATCCTCCTTTAG	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29110_Hsan_Wil2021_COI	PLO-B24	1	BD29110_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	CCTGGGCCGGTATAGTAGGT	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29110_Mros_Gib2022_COI	PLO-B24	1	BD29110_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29110_Msal_Bot2012_COI	PLO-B24	1	BD29110_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	GYTAGTTCCRATGATGTTAGCTG	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29110_Mstr_Wil2021_COI	PLO-B24	1	BD29110_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	GTAGTGGTAACTACTCATGCTTTA	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29110_Pvir_Dia2013_COI	PLO-B24	1	BD29110_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	CTTAGTGGCATTAATTCGDAATCC	135.86741
BD29110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29110	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29110_Rhar_Sim2018_COI	PLO-B24	1	BD29110_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29110	Sample	BD29110	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B24	COI	45	BD29110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B24	CTGCTGCTATTGCTCATG	135.86741
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29112_Aamu_Bax2006_COI	PLO-B22	1	BD29112_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	GCACAACCGGGATCTTTACTTC	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29112_Pcan_Bot2011_IGS	PLO-B22	1	BD29112_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B22	IGS	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	CGTAATCCTCAGTACTGGCTA	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29112_Pper_Dia2013_COI	PLO-B22	1	BD29112_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	CTTAGTGGCATTAATTCGDAATCC	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29112_Sspa_Oph2007_COI	PLO-B22	1	BD29112_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	GAAGGCCTTCCGATGATC	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29112_Upin_Bot2011_COI	PLO-B22	1	BD29112_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	CTTTAATTACAGCGTTTTTATTGTTGT	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71962	WGS84	BD29112	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29112_Vgib_Wil2025_COI	PLO-B22	1	BD29112_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	CCCCTCTTTACAAGTTCTTTAGGGT	135.86435
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71159	WGS84	BD29113	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29113_Asen_Wil2025_COI	PLO-B21	1	BD29113_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	GTTGTTGTTACAACACATGCTCTTTTG	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29113_Cjap_Sim2018_COI	PLO-B21	1	BD29113_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29113_Esin_And2018_Cyb	PLO-B21	1	BD29113_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B21	Cytb	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	ACCCCTCCTCATATCCAACCA	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29113_Mgig_Bot2012_COI	PLO-B21	1	BD29113_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	TCTTATTCGTTGGAGACTTTATAACCCT	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29113_Mjap_Gib2022_COI	PLO-B21	1	BD29113_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	AATTTAATTGTGACTAGGCATGGTCTG	135.86744
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29114_Cmae_Bot2011_COI	PLO-B20	1	BD29114_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	ATGAACAGTCTATCCTCCTTTAG	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29114_Hsan_Wil2021_COI	PLO-B20	1	BD29114_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	CCTGGGCCGGTATAGTAGGT	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29114_Mros_Gib2022_COI	PLO-B20	1	BD29114_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29114_Msal_Bot2012_COI	PLO-B20	1	BD29114_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	GYTAGTTCCRATGATGTTAGCTG	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29114_Mstr_Wil2021_COI	PLO-B20	1	BD29114_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	GTAGTGGTAACTACTCATGCTTTA	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29114_Pvir_Dia2013_COI	PLO-B20	1	BD29114_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	CTTAGTGGCATTAATTCGDAATCC	135.8694
BD29114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71153	WGS84	BD29114	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29114_Rhar_Sim2018_COI	PLO-B20	1	BD29114_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29114	Sample	BD29114	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B20	COI	45	BD29114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B20	CTGCTGCTATTGCTCATG	135.8694
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29116_Aamu_Bax2006_COI	PLO-B18	1	BD29116_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	GCACAACCGGGATCTTTACTTC	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29116_Pcan_Bot2011_IGS	PLO-B18	1	BD29116_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B18	IGS	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	CGTAATCCTCAGTACTGGCTA	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29116_Pper_Dia2013_COI	PLO-B18	1	BD29116_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	CTTAGTGGCATTAATTCGDAATCC	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29116_Sspa_Oph2007_COI	PLO-B18	1	BD29116_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	GAAGGCCTTCCGATGATC	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29116_Upin_Bot2011_COI	PLO-B18	1	BD29116_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	CTTTAATTACAGCGTTTTTATTGTTGT	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71148	WGS84	BD29116	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29116_Vgib_Wil2025_COI	PLO-B18	1	BD29116_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	CCCCTCTTTACAAGTTCTTTAGGGT	135.87388
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71303	WGS84	BD29117	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29117_Asen_Wil2025_COI	PLO-B17	1	BD29117_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	GTTGTTGTTACAACACATGCTCTTTTG	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29117_Cjap_Sim2018_COI	PLO-B17	1	BD29117_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29117_Esin_And2018_Cyb	PLO-B17	1	BD29117_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B17	Cytb	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	ACCCCTCCTCATATCCAACCA	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29117_Mgig_Bot2012_COI	PLO-B17	1	BD29117_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	TCTTATTCGTTGGAGACTTTATAACCCT	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29117_Mjap_Gib2022_COI	PLO-B17	1	BD29117_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	AATTTAATTGTGACTAGGCATGGTCTG	135.87506
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29118_Cmae_Bot2011_COI	PLO-B16	1	BD29118_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	ATGAACAGTCTATCCTCCTTTAG	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29118_Hsan_Wil2021_COI	PLO-B16	1	BD29118_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	CCTGGGCCGGTATAGTAGGT	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29118_Mros_Gib2022_COI	PLO-B16	1	BD29118_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29118_Msal_Bot2012_COI	PLO-B16	1	BD29118_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	GYTAGTTCCRATGATGTTAGCTG	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29118_Mstr_Wil2021_COI	PLO-B16	1	BD29118_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	GTAGTGGTAACTACTCATGCTTTA	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29118_Pvir_Dia2013_COI	PLO-B16	1	BD29118_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	CTTAGTGGCATTAATTCGDAATCC	135.87288
BD29118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71312	WGS84	BD29118	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29118_Rhar_Sim2018_COI	PLO-B16	1	BD29118_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29118	Sample	BD29118	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B16	COI	45	BD29118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B16	CTGCTGCTATTGCTCATG	135.87288
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29120_Cjap_Sim2018_COI	PLO-B14	1	BD29120_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29120_Esin_And2018_Cyb	PLO-B14	1	BD29120_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B14	Cytb	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	ACCCCTCCTCATATCCAACCA	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29120_Mgig_Bot2012_COI	PLO-B14	1	BD29120_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	TCTTATTCGTTGGAGACTTTATAACCCT	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29120_Mjap_Gib2022_COI	PLO-B14	1	BD29120_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	AATTTAATTGTGACTAGGCATGGTCTG	135.87404
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29121_Cmae_Bot2011_COI	PLO-B13	1	BD29121_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	ATGAACAGTCTATCCTCCTTTAG	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29121_Hsan_Wil2021_COI	PLO-B13	1	BD29121_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	CCTGGGCCGGTATAGTAGGT	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29121_Mros_Gib2022_COI	PLO-B13	1	BD29121_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29121_Msal_Bot2012_COI	PLO-B13	1	BD29121_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	GYTAGTTCCRATGATGTTAGCTG	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29121_Mstr_Wil2021_COI	PLO-B13	1	BD29121_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	GTAGTGGTAACTACTCATGCTTTA	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29121_Pvir_Dia2013_COI	PLO-B13	1	BD29121_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	CTTAGTGGCATTAATTCGDAATCC	135.87618
BD29121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71464	WGS84	BD29121	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29121_Rhar_Sim2018_COI	PLO-B13	1	BD29121_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29121	Sample	BD29121	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B13	COI	45	BD29121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B13	CTGCTGCTATTGCTCATG	135.87618
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29123_Aamu_Bax2006_COI	PLO-B11	1	BD29123_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	GCACAACCGGGATCTTTACTTC	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29123_Pcan_Bot2011_IGS	PLO-B11	1	BD29123_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B11	IGS	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	CGTAATCCTCAGTACTGGCTA	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29123_Pper_Dia2013_COI	PLO-B11	1	BD29123_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	CTTAGTGGCATTAATTCGDAATCC	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29123_Sspa_Oph2007_COI	PLO-B11	1	BD29123_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	GAAGGCCTTCCGATGATC	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29123_Upin_Bot2011_COI	PLO-B11	1	BD29123_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	CTTTAATTACAGCGTTTTTATTGTTGT	135.87959
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29124_Cjap_Sim2018_COI	PLO-B10	1	BD29124_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29124_Esin_And2018_Cyb	PLO-B10	1	BD29124_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B10	Cytb	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	ACCCCTCCTCATATCCAACCA	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29124_Mgig_Bot2012_COI	PLO-B10	1	BD29124_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	TCTTATTCGTTGGAGACTTTATAACCCT	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29124_Mjap_Gib2022_COI	PLO-B10	1	BD29124_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	AATTTAATTGTGACTAGGCATGGTCTG	135.87733
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29125_Cmae_Bot2011_COI	PLO-B09	1	BD29125_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	ATGAACAGTCTATCCTCCTTTAG	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29125_Hsan_Wil2021_COI	PLO-B09	1	BD29125_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	CCTGGGCCGGTATAGTAGGT	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29125_Mros_Gib2022_COI	PLO-B09	1	BD29125_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29125_Msal_Bot2012_COI	PLO-B09	1	BD29125_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	GYTAGTTCCRATGATGTTAGCTG	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29125_Mstr_Wil2021_COI	PLO-B09	1	BD29125_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	GTAGTGGTAACTACTCATGCTTTA	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29125_Pvir_Dia2013_COI	PLO-B09	1	BD29125_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	CTTAGTGGCATTAATTCGDAATCC	135.87514
BD29125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71623	WGS84	BD29125	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29125_Rhar_Sim2018_COI	PLO-B09	1	BD29125_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29125	Sample	BD29125	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B09	COI	45	BD29125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B09	CTGCTGCTATTGCTCATG	135.87514
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29127_Aamu_Bax2006_COI	PLO-B07	1	BD29127_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	GCACAACCGGGATCTTTACTTC	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29127_Pcan_Bot2011_IGS	PLO-B07	1	BD29127_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B07	IGS	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	CGTAATCCTCAGTACTGGCTA	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29127_Pper_Dia2013_COI	PLO-B07	1	BD29127_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	CTTAGTGGCATTAATTCGDAATCC	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.52	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29127_Sspa_Oph2007_COI	PLO-B07	1	BD29127_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	GAAGGCCTTCCGATGATC	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29127_Upin_Bot2011_COI	PLO-B07	1	BD29127_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	CTTTAATTACAGCGTTTTTATTGTTGT	135.8712
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29128_Cjap_Sim2018_COI	PLO-B06	1	BD29128_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29128_Esin_And2018_Cyb	PLO-B06	1	BD29128_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B06	Cytb	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	ACCCCTCCTCATATCCAACCA	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29128_Mgig_Bot2012_COI	PLO-B06	1	BD29128_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	TCTTATTCGTTGGAGACTTTATAACCCT	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29128_Mjap_Gib2022_COI	PLO-B06	1	BD29128_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	AATTTAATTGTGACTAGGCATGGTCTG	135.8719
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29129_Cmae_Bot2011_COI	PLO-B05	1	BD29129_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	ATGAACAGTCTATCCTCCTTTAG	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29129_Hsan_Wil2021_COI	PLO-B05	1	BD29129_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	CCTGGGCCGGTATAGTAGGT	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29129_Mros_Gib2022_COI	PLO-B05	1	BD29129_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29129_Msal_Bot2012_COI	PLO-B05	1	BD29129_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	GYTAGTTCCRATGATGTTAGCTG	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29129_Mstr_Wil2021_COI	PLO-B05	1	BD29129_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	GTAGTGGTAACTACTCATGCTTTA	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29129_Pvir_Dia2013_COI	PLO-B05	1	BD29129_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	CTTAGTGGCATTAATTCGDAATCC	135.87293
BD29129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71942	WGS84	BD29129	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29129_Rhar_Sim2018_COI	PLO-B05	1	BD29129_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29129	Sample	BD29129	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B05	COI	45	BD29129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B05	CTGCTGCTATTGCTCATG	135.87293
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29130_Aamu_Bax2006_COI	PLO-B04	1	BD29130_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	GCACAACCGGGATCTTTACTTC	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29130_Pcan_Bot2011_IGS	PLO-B04	1	BD29130_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B04	IGS	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	CGTAATCCTCAGTACTGGCTA	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29130_Pper_Dia2013_COI	PLO-B04	1	BD29130_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	CTTAGTGGCATTAATTCGDAATCC	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29130_Sspa_Oph2007_COI	PLO-B04	1	BD29130_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	GAAGGCCTTCCGATGATC	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29130_Upin_Bot2011_COI	PLO-B04	1	BD29130_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	CTTTAATTACAGCGTTTTTATTGTTGT	135.87408
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29131_Cjap_Sim2018_COI	PLO-B03	1	BD29131_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29131_Esin_And2018_Cyb	PLO-B03	1	BD29131_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B03	Cytb	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	ACCCCTCCTCATATCCAACCA	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29131_Mgig_Bot2012_COI	PLO-B03	1	BD29131_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	TCTTATTCGTTGGAGACTTTATAACCCT	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29131_Mjap_Gib2022_COI	PLO-B03	1	BD29131_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	AATTTAATTGTGACTAGGCATGGTCTG	135.87627
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29132_Cmae_Bot2011_COI	PLO-B02	1	BD29132_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	ATGAACAGTCTATCCTCCTTTAG	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29132_Hsan_Wil2021_COI	PLO-B02	1	BD29132_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	CCTGGGCCGGTATAGTAGGT	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29132_Mros_Gib2022_COI	PLO-B02	1	BD29132_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29132_Msal_Bot2012_COI	PLO-B02	1	BD29132_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	GYTAGTTCCRATGATGTTAGCTG	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29132_Mstr_Wil2021_COI	PLO-B02	1	BD29132_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	GTAGTGGTAACTACTCATGCTTTA	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29132_Pvir_Dia2013_COI	PLO-B02	1	BD29132_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	CTTAGTGGCATTAATTCGDAATCC	135.87846
BD29132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71778	WGS84	BD29132	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29132_Rhar_Sim2018_COI	PLO-B02	1	BD29132_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29132	Sample	BD29132	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B02	COI	45	BD29132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B02	CTGCTGCTATTGCTCATG	135.87846
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29134_Aamu_Bax2006_COI	ADL-A70	1	BD29134_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	GCACAACCGGGATCTTTACTTC	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29134_Pcan_Bot2011_IGS	ADL-A70	1	BD29134_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A70	IGS	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	CGTAATCCTCAGTACTGGCTA	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29134_Pper_Dia2013_COI	ADL-A70	1	BD29134_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	CTTAGTGGCATTAATTCGDAATCC	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.276	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29134_Sspa_Oph2007_COI	ADL-A70	1	BD29134_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	GAAGGCCTTCCGATGATC	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29134_Upin_Bot2011_COI	ADL-A70	1	BD29134_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	CTTTAATTACAGCGTTTTTATTGTTGT	138.51415
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29135_Cjap_Sim2018_COI	ADL-A69	1	BD29135_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29135_Esin_And2018_Cyb	ADL-A69	1	BD29135_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A69	Cytb	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	ACCCCTCCTCATATCCAACCA	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.261	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29135_Mgig_Bot2012_COI	ADL-A69	1	BD29135_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	TCTTATTCGTTGGAGACTTTATAACCCT	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29135_Mjap_Gib2022_COI	ADL-A69	1	BD29135_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	AATTTAATTGTGACTAGGCATGGTCTG	138.51371
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.376	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29136_Cmae_Bot2011_COI	ADL-A68	1	BD29136_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	ATGAACAGTCTATCCTCCTTTAG	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29136_Hsan_Wil2021_COI	ADL-A68	1	BD29136_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	CCTGGGCCGGTATAGTAGGT	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29136_Mros_Gib2022_COI	ADL-A68	1	BD29136_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29136_Msal_Bot2012_COI	ADL-A68	1	BD29136_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	GYTAGTTCCRATGATGTTAGCTG	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29136_Mstr_Wil2021_COI	ADL-A68	1	BD29136_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	GTAGTGGTAACTACTCATGCTTTA	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29136_Pvir_Dia2013_COI	ADL-A68	1	BD29136_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	CTTAGTGGCATTAATTCGDAATCC	138.51366
BD29136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81425	WGS84	BD29136	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29136_Rhar_Sim2018_COI	ADL-A68	1	BD29136_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29136	Sample	BD29136	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A68	COI	45	BD29136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A68	CTGCTGCTATTGCTCATG	138.51366
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29138_Aamu_Bax2006_COI	ADL-A66	1	BD29138_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	GCACAACCGGGATCTTTACTTC	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29138_Pcan_Bot2011_IGS	ADL-A66	1	BD29138_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A66	IGS	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	CGTAATCCTCAGTACTGGCTA	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29138_Pper_Dia2013_COI	ADL-A66	1	BD29138_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	CTTAGTGGCATTAATTCGDAATCC	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.389	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29138_Sspa_Oph2007_COI	ADL-A66	1	BD29138_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	GAAGGCCTTCCGATGATC	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29138_Upin_Bot2011_COI	ADL-A66	1	BD29138_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	CTTTAATTACAGCGTTTTTATTGTTGT	138.51382
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29139_Cjap_Sim2018_COI	ADL-A65	1	BD29139_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29139_Esin_And2018_Cyb	ADL-A65	1	BD29139_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A65	Cytb	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	ACCCCTCCTCATATCCAACCA	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29139_Mgig_Bot2012_COI	ADL-A65	1	BD29139_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	TCTTATTCGTTGGAGACTTTATAACCCT	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29139_Mjap_Gib2022_COI	ADL-A65	1	BD29139_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	AATTTAATTGTGACTAGGCATGGTCTG	138.51317
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29141_Aamu_Bax2006_COI	ADL-A63	1	BD29141_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	GCACAACCGGGATCTTTACTTC	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29141_Pcan_Bot2011_IGS	ADL-A63	1	BD29141_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A63	IGS	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	CGTAATCCTCAGTACTGGCTA	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29141_Pper_Dia2013_COI	ADL-A63	1	BD29141_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	CTTAGTGGCATTAATTCGDAATCC	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.981	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29141_Sspa_Oph2007_COI	ADL-A63	1	BD29141_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	GAAGGCCTTCCGATGATC	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29141_Upin_Bot2011_COI	ADL-A63	1	BD29141_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	CTTTAATTACAGCGTTTTTATTGTTGT	138.51165
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29142_Cjap_Sim2018_COI	ADL-A62	1	BD29142_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29142_Esin_And2018_Cyb	ADL-A62	1	BD29142_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A62	Cytb	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	ACCCCTCCTCATATCCAACCA	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29142_Mgig_Bot2012_COI	ADL-A62	1	BD29142_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	TCTTATTCGTTGGAGACTTTATAACCCT	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29142_Mjap_Gib2022_COI	ADL-A62	1	BD29142_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	AATTTAATTGTGACTAGGCATGGTCTG	138.51129
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.844	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29143_Cmae_Bot2011_COI	ADL-A61	1	BD29143_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	ATGAACAGTCTATCCTCCTTTAG	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29143_Hsan_Wil2021_COI	ADL-A61	1	BD29143_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	CCTGGGCCGGTATAGTAGGT	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29143_Mros_Gib2022_COI	ADL-A61	1	BD29143_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29143_Msal_Bot2012_COI	ADL-A61	1	BD29143_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	GYTAGTTCCRATGATGTTAGCTG	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29143_Mstr_Wil2021_COI	ADL-A61	1	BD29143_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	GTAGTGGTAACTACTCATGCTTTA	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29143_Pvir_Dia2013_COI	ADL-A61	1	BD29143_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	CTTAGTGGCATTAATTCGDAATCC	138.51199
BD29143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82142	WGS84	BD29143	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29143_Rhar_Sim2018_COI	ADL-A61	1	BD29143_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29143	Sample	BD29143	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A61	COI	45	BD29143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A61	CTGCTGCTATTGCTCATG	138.51199
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29145_Aamu_Bax2006_COI	ADL-A59	1	BD29145_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	GCACAACCGGGATCTTTACTTC	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29145_Pcan_Bot2011_IGS	ADL-A59	1	BD29145_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A59	IGS	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	CGTAATCCTCAGTACTGGCTA	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29145_Pper_Dia2013_COI	ADL-A59	1	BD29145_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	CTTAGTGGCATTAATTCGDAATCC	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.235	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29145_Sspa_Oph2007_COI	ADL-A59	1	BD29145_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	GAAGGCCTTCCGATGATC	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29145_Upin_Bot2011_COI	ADL-A59	1	BD29145_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	CTTTAATTACAGCGTTTTTATTGTTGT	138.51145
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29146_Cjap_Sim2018_COI	ADL-A58	1	BD29146_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29146_Esin_And2018_Cyb	ADL-A58	1	BD29146_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A58	Cytb	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	ACCCCTCCTCATATCCAACCA	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29146_Mgig_Bot2012_COI	ADL-A58	1	BD29146_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	TCTTATTCGTTGGAGACTTTATAACCCT	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29146_Mjap_Gib2022_COI	ADL-A58	1	BD29146_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	AATTTAATTGTGACTAGGCATGGTCTG	138.51002
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.727	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29147_Cmae_Bot2011_COI	ADL-A57	1	BD29147_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	ATGAACAGTCTATCCTCCTTTAG	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29147_Hsan_Wil2021_COI	ADL-A57	1	BD29147_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	CCTGGGCCGGTATAGTAGGT	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29147_Mros_Gib2022_COI	ADL-A57	1	BD29147_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29147_Msal_Bot2012_COI	ADL-A57	1	BD29147_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	GYTAGTTCCRATGATGTTAGCTG	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29147_Mstr_Wil2021_COI	ADL-A57	1	BD29147_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	GTAGTGGTAACTACTCATGCTTTA	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29147_Pvir_Dia2013_COI	ADL-A57	1	BD29147_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	CTTAGTGGCATTAATTCGDAATCC	138.51067
BD29147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82351	WGS84	BD29147	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29147_Rhar_Sim2018_COI	ADL-A57	1	BD29147_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29147	Sample	BD29147	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A57	COI	45	BD29147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A57	CTGCTGCTATTGCTCATG	138.51067
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29149_Aamu_Bax2006_COI	ADL-A55	1	BD29149_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	GCACAACCGGGATCTTTACTTC	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29149_Pcan_Bot2011_IGS	ADL-A55	1	BD29149_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A55	IGS	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	CGTAATCCTCAGTACTGGCTA	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29149_Pper_Dia2013_COI	ADL-A55	1	BD29149_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	CTTAGTGGCATTAATTCGDAATCC	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.843	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29149_Sspa_Oph2007_COI	ADL-A55	1	BD29149_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	GAAGGCCTTCCGATGATC	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29149_Upin_Bot2011_COI	ADL-A55	1	BD29149_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	CTTTAATTACAGCGTTTTTATTGTTGT	138.51001
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29150_Cmae_Bot2011_COI	ADL-A54	1	BD29150_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	ATGAACAGTCTATCCTCCTTTAG	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29150_Hsan_Wil2021_COI	ADL-A54	1	BD29150_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	CCTGGGCCGGTATAGTAGGT	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29150_Mros_Gib2022_COI	ADL-A54	1	BD29150_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29150_Msal_Bot2012_COI	ADL-A54	1	BD29150_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	GYTAGTTCCRATGATGTTAGCTG	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29150_Mstr_Wil2021_COI	ADL-A54	1	BD29150_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	GTAGTGGTAACTACTCATGCTTTA	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29150_Pvir_Dia2013_COI	ADL-A54	1	BD29150_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	CTTAGTGGCATTAATTCGDAATCC	138.50873
BD29150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82456	WGS84	BD29150	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29150_Rhar_Sim2018_COI	ADL-A54	1	BD29150_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29150	Sample	BD29150	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A54	COI	45	BD29150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A54	CTGCTGCTATTGCTCATG	138.50873
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29152_Aamu_Bax2006_COI	ADL-A52	1	BD29152_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	GCACAACCGGGATCTTTACTTC	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29152_Pcan_Bot2011_IGS	ADL-A52	1	BD29152_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A52	IGS	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	CGTAATCCTCAGTACTGGCTA	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29152_Pper_Dia2013_COI	ADL-A52	1	BD29152_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	CTTAGTGGCATTAATTCGDAATCC	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.375	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29152_Sspa_Oph2007_COI	ADL-A52	1	BD29152_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	GAAGGCCTTCCGATGATC	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29152_Upin_Bot2011_COI	ADL-A52	1	BD29152_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	CTTTAATTACAGCGTTTTTATTGTTGT	138.50802
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29153_Cjap_Sim2018_COI	ADL-A51	1	BD29153_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29153_Esin_And2018_Cyb	ADL-A51	1	BD29153_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A51	Cytb	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	ACCCCTCCTCATATCCAACCA	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29153_Mgig_Bot2012_COI	ADL-A51	1	BD29153_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	TCTTATTCGTTGGAGACTTTATAACCCT	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29153_Mjap_Gib2022_COI	ADL-A51	1	BD29153_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	AATTTAATTGTGACTAGGCATGGTCTG	138.50876
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29154_Cmae_Bot2011_COI	ADL-A50	1	BD29154_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	ATGAACAGTCTATCCTCCTTTAG	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29154_Hsan_Wil2021_COI	ADL-A50	1	BD29154_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	CCTGGGCCGGTATAGTAGGT	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29154_Mros_Gib2022_COI	ADL-A50	1	BD29154_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29154_Msal_Bot2012_COI	ADL-A50	1	BD29154_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	GYTAGTTCCRATGATGTTAGCTG	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29154_Mstr_Wil2021_COI	ADL-A50	1	BD29154_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	GTAGTGGTAACTACTCATGCTTTA	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29154_Pvir_Dia2013_COI	ADL-A50	1	BD29154_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	CTTAGTGGCATTAATTCGDAATCC	138.5073
BD29154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82667	WGS84	BD29154	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29154_Rhar_Sim2018_COI	ADL-A50	1	BD29154_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29154	Sample	BD29154	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A50	COI	45	BD29154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A50	CTGCTGCTATTGCTCATG	138.5073
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29156_Aamu_Bax2006_COI	ADL-A48	1	BD29156_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	GCACAACCGGGATCTTTACTTC	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29156_Pcan_Bot2011_IGS	ADL-A48	1	BD29156_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A48	IGS	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	CGTAATCCTCAGTACTGGCTA	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29156_Pper_Dia2013_COI	ADL-A48	1	BD29156_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	CTTAGTGGCATTAATTCGDAATCC	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.179	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29156_Sspa_Oph2007_COI	ADL-A48	1	BD29156_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	GAAGGCCTTCCGATGATC	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29156_Upin_Bot2011_COI	ADL-A48	1	BD29156_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	CTTTAATTACAGCGTTTTTATTGTTGT	138.50888
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29157_Cjap_Sim2018_COI	ADL-A47	1	BD29157_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29157_Esin_And2018_Cyb	ADL-A47	1	BD29157_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A47	Cytb	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	ACCCCTCCTCATATCCAACCA	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29157_Mgig_Bot2012_COI	ADL-A47	1	BD29157_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	TCTTATTCGTTGGAGACTTTATAACCCT	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29157_Mjap_Gib2022_COI	ADL-A47	1	BD29157_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	AATTTAATTGTGACTAGGCATGGTCTG	138.50732
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29158_Cmae_Bot2011_COI	ADL-A46	1	BD29158_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	ATGAACAGTCTATCCTCCTTTAG	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29158_Hsan_Wil2021_COI	ADL-A46	1	BD29158_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	CCTGGGCCGGTATAGTAGGT	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29158_Mros_Gib2022_COI	ADL-A46	1	BD29158_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29158_Msal_Bot2012_COI	ADL-A46	1	BD29158_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	GYTAGTTCCRATGATGTTAGCTG	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29158_Mstr_Wil2021_COI	ADL-A46	1	BD29158_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	GTAGTGGTAACTACTCATGCTTTA	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29158_Pvir_Dia2013_COI	ADL-A46	1	BD29158_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	CTTAGTGGCATTAATTCGDAATCC	138.50951
BD29158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82965	WGS84	BD29158	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29158_Rhar_Sim2018_COI	ADL-A46	1	BD29158_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29158	Sample	BD29158	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A46	COI	45	BD29158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A46	CTGCTGCTATTGCTCATG	138.50951
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29160_Cjap_Sim2018_COI	ADL-A44	1	BD29160_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29160_Esin_And2018_Cyb	ADL-A44	1	BD29160_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A44	Cytb	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	ACCCCTCCTCATATCCAACCA	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29160_Mgig_Bot2012_COI	ADL-A44	1	BD29160_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	TCTTATTCGTTGGAGACTTTATAACCCT	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29160_Mjap_Gib2022_COI	ADL-A44	1	BD29160_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	AATTTAATTGTGACTAGGCATGGTCTG	138.50737
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.109	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29161_Cmae_Bot2011_COI	ADL-A43	1	BD29161_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	ATGAACAGTCTATCCTCCTTTAG	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29161_Hsan_Wil2021_COI	ADL-A43	1	BD29161_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	CCTGGGCCGGTATAGTAGGT	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29161_Mros_Gib2022_COI	ADL-A43	1	BD29161_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29161_Msal_Bot2012_COI	ADL-A43	1	BD29161_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	GYTAGTTCCRATGATGTTAGCTG	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29161_Mstr_Wil2021_COI	ADL-A43	1	BD29161_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	GTAGTGGTAACTACTCATGCTTTA	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29161_Pvir_Dia2013_COI	ADL-A43	1	BD29161_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	CTTAGTGGCATTAATTCGDAATCC	138.50739
BD29161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8328	WGS84	BD29161	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29161_Rhar_Sim2018_COI	ADL-A43	1	BD29161_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29161	Sample	BD29161	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A43	COI	45	BD29161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A43	CTGCTGCTATTGCTCATG	138.50739
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29163_Aamu_Bax2006_COI	ADL-A41	1	BD29163_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	GCACAACCGGGATCTTTACTTC	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29163_Pcan_Bot2011_IGS	ADL-A41	1	BD29163_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A41	IGS	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	CGTAATCCTCAGTACTGGCTA	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29163_Pper_Dia2013_COI	ADL-A41	1	BD29163_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	CTTAGTGGCATTAATTCGDAATCC	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.533	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29163_Sspa_Oph2007_COI	ADL-A41	1	BD29163_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	GAAGGCCTTCCGATGATC	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29163_Upin_Bot2011_COI	ADL-A41	1	BD29163_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	CTTTAATTACAGCGTTTTTATTGTTGT	138.50654
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29164_Cjap_Sim2018_COI	ADL-A40	1	BD29164_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29164_Esin_And2018_Cyb	ADL-A40	1	BD29164_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A40	Cytb	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	ACCCCTCCTCATATCCAACCA	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29164_Mgig_Bot2012_COI	ADL-A40	1	BD29164_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	TCTTATTCGTTGGAGACTTTATAACCCT	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29164_Mjap_Gib2022_COI	ADL-A40	1	BD29164_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	AATTTAATTGTGACTAGGCATGGTCTG	138.50533
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.637	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29165_Cmae_Bot2011_COI	ADL-A39	1	BD29165_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	ATGAACAGTCTATCCTCCTTTAG	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29165_Hsan_Wil2021_COI	ADL-A39	1	BD29165_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	CCTGGGCCGGTATAGTAGGT	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29165_Mros_Gib2022_COI	ADL-A39	1	BD29165_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29165_Msal_Bot2012_COI	ADL-A39	1	BD29165_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	GYTAGTTCCRATGATGTTAGCTG	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29165_Mstr_Wil2021_COI	ADL-A39	1	BD29165_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	GTAGTGGTAACTACTCATGCTTTA	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29165_Pvir_Dia2013_COI	ADL-A39	1	BD29165_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	CTTAGTGGCATTAATTCGDAATCC	138.50579
BD29165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83993	WGS84	BD29165	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29165_Rhar_Sim2018_COI	ADL-A39	1	BD29165_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29165	Sample	BD29165	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A39	COI	45	BD29165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A39	CTGCTGCTATTGCTCATG	138.50579
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29167_Aamu_Bax2006_COI	ADL-A37	1	BD29167_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	GCACAACCGGGATCTTTACTTC	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29167_Pcan_Bot2011_IGS	ADL-A37	1	BD29167_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A37	IGS	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	CGTAATCCTCAGTACTGGCTA	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29167_Pper_Dia2013_COI	ADL-A37	1	BD29167_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	CTTAGTGGCATTAATTCGDAATCC	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.092	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29167_Sspa_Oph2007_COI	ADL-A37	1	BD29167_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	GAAGGCCTTCCGATGATC	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29167_Upin_Bot2011_COI	ADL-A37	1	BD29167_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	CTTTAATTACAGCGTTTTTATTGTTGT	138.50054
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29168_Cjap_Sim2018_COI	ADL-A36	1	BD29168_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29168_Esin_And2018_Cyb	ADL-A36	1	BD29168_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A36	Cytb	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	ACCCCTCCTCATATCCAACCA	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29168_Mgig_Bot2012_COI	ADL-A36	1	BD29168_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	TCTTATTCGTTGGAGACTTTATAACCCT	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29168_Mjap_Gib2022_COI	ADL-A36	1	BD29168_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	AATTTAATTGTGACTAGGCATGGTCTG	138.4992
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29169_Cmae_Bot2011_COI	ADL-A35	1	BD29169_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	ATGAACAGTCTATCCTCCTTTAG	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29169_Hsan_Wil2021_COI	ADL-A35	1	BD29169_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	CCTGGGCCGGTATAGTAGGT	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29169_Mros_Gib2022_COI	ADL-A35	1	BD29169_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29169_Msal_Bot2012_COI	ADL-A35	1	BD29169_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	GYTAGTTCCRATGATGTTAGCTG	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29169_Mstr_Wil2021_COI	ADL-A35	1	BD29169_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	GTAGTGGTAACTACTCATGCTTTA	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29169_Pvir_Dia2013_COI	ADL-A35	1	BD29169_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	CTTAGTGGCATTAATTCGDAATCC	138.5
BD29169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76058	WGS84	BD29169	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29169_Rhar_Sim2018_COI	ADL-A35	1	BD29169_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29169	Sample	BD29169	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A35	COI	45	BD29169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A35	CTGCTGCTATTGCTCATG	138.5
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29170_Aamu_Bax2006_COI	ADL-A34	1	BD29170_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	GCACAACCGGGATCTTTACTTC	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29170_Pcan_Bot2011_IGS	ADL-A34	1	BD29170_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A34	IGS	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	CGTAATCCTCAGTACTGGCTA	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29170_Pper_Dia2013_COI	ADL-A34	1	BD29170_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	CTTAGTGGCATTAATTCGDAATCC	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.558	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29170_Sspa_Oph2007_COI	ADL-A34	1	BD29170_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	GAAGGCCTTCCGATGATC	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29170_Upin_Bot2011_COI	ADL-A34	1	BD29170_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	CTTTAATTACAGCGTTTTTATTGTTGT	138.49805
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29171_Cjap_Sim2018_COI	ADL-A33	1	BD29171_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29171_Esin_And2018_Cyb	ADL-A33	1	BD29171_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A33	Cytb	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	ACCCCTCCTCATATCCAACCA	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29171_Mgig_Bot2012_COI	ADL-A33	1	BD29171_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	TCTTATTCGTTGGAGACTTTATAACCCT	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29171_Mjap_Gib2022_COI	ADL-A33	1	BD29171_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	AATTTAATTGTGACTAGGCATGGTCTG	138.5011
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.504	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29172_Cmae_Bot2011_COI	ADL-A32	1	BD29172_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	ATGAACAGTCTATCCTCCTTTAG	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29172_Hsan_Wil2021_COI	ADL-A32	1	BD29172_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	CCTGGGCCGGTATAGTAGGT	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29172_Mros_Gib2022_COI	ADL-A32	1	BD29172_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29172_Msal_Bot2012_COI	ADL-A32	1	BD29172_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	GYTAGTTCCRATGATGTTAGCTG	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29172_Mstr_Wil2021_COI	ADL-A32	1	BD29172_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	GTAGTGGTAACTACTCATGCTTTA	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29172_Pvir_Dia2013_COI	ADL-A32	1	BD29172_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	CTTAGTGGCATTAATTCGDAATCC	138.49913
BD29172	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76238	WGS84	BD29172	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29172_Rhar_Sim2018_COI	ADL-A32	1	BD29172_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29172	Sample	BD29172	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A32	COI	45	BD29172	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A32	CTGCTGCTATTGCTCATG	138.49913
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29174_Aamu_Bax2006_COI	ADL-A30	1	BD29174_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	GCACAACCGGGATCTTTACTTC	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29174_Pcan_Bot2011_IGS	ADL-A30	1	BD29174_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A30	IGS	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	CGTAATCCTCAGTACTGGCTA	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29174_Pper_Dia2013_COI	ADL-A30	1	BD29174_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	CTTAGTGGCATTAATTCGDAATCC	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.283	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29174_Sspa_Oph2007_COI	ADL-A30	1	BD29174_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	GAAGGCCTTCCGATGATC	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29174_Upin_Bot2011_COI	ADL-A30	1	BD29174_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	CTTTAATTACAGCGTTTTTATTGTTGT	138.49501
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29175_Cjap_Sim2018_COI	ADL-A29	1	BD29175_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29175_Esin_And2018_Cyb	ADL-A29	1	BD29175_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A29	Cytb	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	ACCCCTCCTCATATCCAACCA	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29175_Mgig_Bot2012_COI	ADL-A29	1	BD29175_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	TCTTATTCGTTGGAGACTTTATAACCCT	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29175_Mjap_Gib2022_COI	ADL-A29	1	BD29175_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	AATTTAATTGTGACTAGGCATGGTCTG	138.49487
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29176_Cmae_Bot2011_COI	ADL-A28	1	BD29176_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	ATGAACAGTCTATCCTCCTTTAG	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29176_Hsan_Wil2021_COI	ADL-A28	1	BD29176_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	CCTGGGCCGGTATAGTAGGT	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29176_Mros_Gib2022_COI	ADL-A28	1	BD29176_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29176_Msal_Bot2012_COI	ADL-A28	1	BD29176_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	GYTAGTTCCRATGATGTTAGCTG	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29176_Mstr_Wil2021_COI	ADL-A28	1	BD29176_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	GTAGTGGTAACTACTCATGCTTTA	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29176_Pvir_Dia2013_COI	ADL-A28	1	BD29176_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	CTTAGTGGCATTAATTCGDAATCC	138.49389
BD29176	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76393	WGS84	BD29176	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29176_Rhar_Sim2018_COI	ADL-A28	1	BD29176_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29176	Sample	BD29176	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A28	COI	45	BD29176	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A28	CTGCTGCTATTGCTCATG	138.49389
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29178_Aamu_Bax2006_COI	ADL-A26	1	BD29178_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	GCACAACCGGGATCTTTACTTC	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29178_Pcan_Bot2011_IGS	ADL-A26	1	BD29178_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A26	IGS	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	CGTAATCCTCAGTACTGGCTA	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29178_Pper_Dia2013_COI	ADL-A26	1	BD29178_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	CTTAGTGGCATTAATTCGDAATCC	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.742	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29178_Sspa_Oph2007_COI	ADL-A26	1	BD29178_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	GAAGGCCTTCCGATGATC	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29178_Upin_Bot2011_COI	ADL-A26	1	BD29178_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	CTTTAATTACAGCGTTTTTATTGTTGT	138.49301
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29179_Cjap_Sim2018_COI	ADL-A25	1	BD29179_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29179_Esin_And2018_Cyb	ADL-A25	1	BD29179_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A25	Cytb	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	ACCCCTCCTCATATCCAACCA	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29179_Mgig_Bot2012_COI	ADL-A25	1	BD29179_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	TCTTATTCGTTGGAGACTTTATAACCCT	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29179_Mjap_Gib2022_COI	ADL-A25	1	BD29179_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	AATTTAATTGTGACTAGGCATGGTCTG	138.49085
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29181_Aamu_Bax2006_COI	ADL-A23	1	BD29181_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	GCACAACCGGGATCTTTACTTC	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29181_Pcan_Bot2011_IGS	ADL-A23	1	BD29181_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A23	IGS	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	CGTAATCCTCAGTACTGGCTA	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29181_Pper_Dia2013_COI	ADL-A23	1	BD29181_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	CTTAGTGGCATTAATTCGDAATCC	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.976	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29181_Sspa_Oph2007_COI	ADL-A23	1	BD29181_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	GAAGGCCTTCCGATGATC	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29181_Upin_Bot2011_COI	ADL-A23	1	BD29181_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	CTTTAATTACAGCGTTTTTATTGTTGT	138.48983
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29182_Cjap_Sim2018_COI	ADL-A22	1	BD29182_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29182_Esin_And2018_Cyb	ADL-A22	1	BD29182_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A22	Cytb	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	ACCCCTCCTCATATCCAACCA	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29182_Mgig_Bot2012_COI	ADL-A22	1	BD29182_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	TCTTATTCGTTGGAGACTTTATAACCCT	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29182_Mjap_Gib2022_COI	ADL-A22	1	BD29182_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	AATTTAATTGTGACTAGGCATGGTCTG	138.48798
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.702	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29183_Cmae_Bot2011_COI	ADL-A21	1	BD29183_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	ATGAACAGTCTATCCTCCTTTAG	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29183_Hsan_Wil2021_COI	ADL-A21	1	BD29183_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	CCTGGGCCGGTATAGTAGGT	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29183_Mros_Gib2022_COI	ADL-A21	1	BD29183_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29183_Msal_Bot2012_COI	ADL-A21	1	BD29183_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	GYTAGTTCCRATGATGTTAGCTG	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29183_Mstr_Wil2021_COI	ADL-A21	1	BD29183_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	GTAGTGGTAACTACTCATGCTTTA	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29183_Pvir_Dia2013_COI	ADL-A21	1	BD29183_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	CTTAGTGGCATTAATTCGDAATCC	138.48607
BD29183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76762	WGS84	BD29183	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29183_Rhar_Sim2018_COI	ADL-A21	1	BD29183_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29183	Sample	BD29183	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A21	COI	45	BD29183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A21	CTGCTGCTATTGCTCATG	138.48607
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29185_Aamu_Bax2006_COI	ADL-A19	1	BD29185_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	GCACAACCGGGATCTTTACTTC	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29185_Pcan_Bot2011_IGS	ADL-A19	1	BD29185_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A19	IGS	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	CGTAATCCTCAGTACTGGCTA	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29185_Pper_Dia2013_COI	ADL-A19	1	BD29185_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	CTTAGTGGCATTAATTCGDAATCC	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.299	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29185_Sspa_Oph2007_COI	ADL-A19	1	BD29185_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	GAAGGCCTTCCGATGATC	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29185_Upin_Bot2011_COI	ADL-A19	1	BD29185_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	CTTTAATTACAGCGTTTTTATTGTTGT	138.48667
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29186_Cjap_Sim2018_COI	ADL-A18	1	BD29186_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29186_Esin_And2018_Cyb	ADL-A18	1	BD29186_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A18	Cytb	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	ACCCCTCCTCATATCCAACCA	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29186_Mgig_Bot2012_COI	ADL-A18	1	BD29186_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	TCTTATTCGTTGGAGACTTTATAACCCT	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29186_Mjap_Gib2022_COI	ADL-A18	1	BD29186_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	AATTTAATTGTGACTAGGCATGGTCTG	138.48482
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.18	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29187_Cmae_Bot2011_COI	ADL-A17	1	BD29187_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	ATGAACAGTCTATCCTCCTTTAG	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29187_Hsan_Wil2021_COI	ADL-A17	1	BD29187_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	CCTGGGCCGGTATAGTAGGT	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29187_Mros_Gib2022_COI	ADL-A17	1	BD29187_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29187_Msal_Bot2012_COI	ADL-A17	1	BD29187_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	GYTAGTTCCRATGATGTTAGCTG	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29187_Mstr_Wil2021_COI	ADL-A17	1	BD29187_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	GTAGTGGTAACTACTCATGCTTTA	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29187_Pvir_Dia2013_COI	ADL-A17	1	BD29187_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	CTTAGTGGCATTAATTCGDAATCC	138.48592
BD29187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77058	WGS84	BD29187	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29187_Rhar_Sim2018_COI	ADL-A17	1	BD29187_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29187	Sample	BD29187	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A17	COI	45	BD29187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A17	CTGCTGCTATTGCTCATG	138.48592
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29189_Aamu_Bax2006_COI	ADL-A15	1	BD29189_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	GCACAACCGGGATCTTTACTTC	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29189_Pcan_Bot2011_IGS	ADL-A15	1	BD29189_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A15	IGS	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	CGTAATCCTCAGTACTGGCTA	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29189_Pper_Dia2013_COI	ADL-A15	1	BD29189_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	CTTAGTGGCATTAATTCGDAATCC	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.684	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29189_Sspa_Oph2007_COI	ADL-A15	1	BD29189_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	GAAGGCCTTCCGATGATC	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29189_Upin_Bot2011_COI	ADL-A15	1	BD29189_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	CTTTAATTACAGCGTTTTTATTGTTGT	138.48498
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29190_Cmae_Bot2011_COI	ADL-A14	1	BD29190_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	ATGAACAGTCTATCCTCCTTTAG	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29190_Hsan_Wil2021_COI	ADL-A14	1	BD29190_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	CCTGGGCCGGTATAGTAGGT	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29190_Mros_Gib2022_COI	ADL-A14	1	BD29190_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29190_Msal_Bot2012_COI	ADL-A14	1	BD29190_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	GYTAGTTCCRATGATGTTAGCTG	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29190_Mstr_Wil2021_COI	ADL-A14	1	BD29190_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	GTAGTGGTAACTACTCATGCTTTA	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29190_Pvir_Dia2013_COI	ADL-A14	1	BD29190_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	CTTAGTGGCATTAATTCGDAATCC	138.48294
BD29190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77223	WGS84	BD29190	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29190_Rhar_Sim2018_COI	ADL-A14	1	BD29190_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29190	Sample	BD29190	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A14	COI	45	BD29190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A14	CTGCTGCTATTGCTCATG	138.48294
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29192_Aamu_Bax2006_COI	ADL-A12	1	BD29192_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	GCACAACCGGGATCTTTACTTC	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29192_Pcan_Bot2011_IGS	ADL-A12	1	BD29192_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A12	IGS	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	CGTAATCCTCAGTACTGGCTA	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29192_Pper_Dia2013_COI	ADL-A12	1	BD29192_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	CTTAGTGGCATTAATTCGDAATCC	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.967	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29192_Sspa_Oph2007_COI	ADL-A12	1	BD29192_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	GAAGGCCTTCCGATGATC	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29192_Upin_Bot2011_COI	ADL-A12	1	BD29192_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	CTTTAATTACAGCGTTTTTATTGTTGT	138.48197
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29193_Cjap_Sim2018_COI	ADL-A11	1	BD29193_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29193_Esin_And2018_Cyb	ADL-A11	1	BD29193_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A11	Cytb	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	ACCCCTCCTCATATCCAACCA	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29193_Mgig_Bot2012_COI	ADL-A11	1	BD29193_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	TCTTATTCGTTGGAGACTTTATAACCCT	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29193_Mjap_Gib2022_COI	ADL-A11	1	BD29193_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	AATTTAATTGTGACTAGGCATGGTCTG	138.48302
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29194_Cmae_Bot2011_COI	ADL-A10	1	BD29194_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	ATGAACAGTCTATCCTCCTTTAG	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29194_Hsan_Wil2021_COI	ADL-A10	1	BD29194_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	CCTGGGCCGGTATAGTAGGT	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29194_Mros_Gib2022_COI	ADL-A10	1	BD29194_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29194_Msal_Bot2012_COI	ADL-A10	1	BD29194_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	GYTAGTTCCRATGATGTTAGCTG	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29194_Mstr_Wil2021_COI	ADL-A10	1	BD29194_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	GTAGTGGTAACTACTCATGCTTTA	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29194_Pvir_Dia2013_COI	ADL-A10	1	BD29194_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	CTTAGTGGCATTAATTCGDAATCC	138.4815
BD29194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77602	WGS84	BD29194	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29194_Rhar_Sim2018_COI	ADL-A10	1	BD29194_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29194	Sample	BD29194	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A10	COI	45	BD29194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A10	CTGCTGCTATTGCTCATG	138.4815
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29196_Aamu_Bax2006_COI	ADL-A08	1	BD29196_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	GCACAACCGGGATCTTTACTTC	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29196_Pcan_Bot2011_IGS	ADL-A08	1	BD29196_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A08	IGS	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	CGTAATCCTCAGTACTGGCTA	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29196_Pper_Dia2013_COI	ADL-A08	1	BD29196_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	CTTAGTGGCATTAATTCGDAATCC	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.813	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29196_Sspa_Oph2007_COI	ADL-A08	1	BD29196_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	GAAGGCCTTCCGATGATC	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29196_Upin_Bot2011_COI	ADL-A08	1	BD29196_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	CTTTAATTACAGCGTTTTTATTGTTGT	138.47978
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29197_Cjap_Sim2018_COI	ADL-A07	1	BD29197_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29197_Esin_And2018_Cyb	ADL-A07	1	BD29197_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A07	Cytb	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	ACCCCTCCTCATATCCAACCA	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29197_Mgig_Bot2012_COI	ADL-A07	1	BD29197_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	TCTTATTCGTTGGAGACTTTATAACCCT	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29197_Mjap_Gib2022_COI	ADL-A07	1	BD29197_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	AATTTAATTGTGACTAGGCATGGTCTG	138.4788
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.171	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29198_Cmae_Bot2011_COI	ADL-A06	1	BD29198_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	ATGAACAGTCTATCCTCCTTTAG	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29198_Hsan_Wil2021_COI	ADL-A06	1	BD29198_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	CCTGGGCCGGTATAGTAGGT	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29198_Mros_Gib2022_COI	ADL-A06	1	BD29198_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29198_Msal_Bot2012_COI	ADL-A06	1	BD29198_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	GYTAGTTCCRATGATGTTAGCTG	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29198_Mstr_Wil2021_COI	ADL-A06	1	BD29198_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	GTAGTGGTAACTACTCATGCTTTA	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29198_Pvir_Dia2013_COI	ADL-A06	1	BD29198_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	CTTAGTGGCATTAATTCGDAATCC	138.47728
BD29198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78055	WGS84	BD29198	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29198_Rhar_Sim2018_COI	ADL-A06	1	BD29198_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29198	Sample	BD29198	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A06	COI	45	BD29198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A06	CTGCTGCTATTGCTCATG	138.47728
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29200_Aamu_Bax2006_COI	ADL-A04	1	BD29200_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	GCACAACCGGGATCTTTACTTC	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29200_Pcan_Bot2011_IGS	ADL-A04	1	BD29200_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A04	IGS	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	CGTAATCCTCAGTACTGGCTA	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29200_Pper_Dia2013_COI	ADL-A04	1	BD29200_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	CTTAGTGGCATTAATTCGDAATCC	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.535	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29200_Sspa_Oph2007_COI	ADL-A04	1	BD29200_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	GAAGGCCTTCCGATGATC	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29200_Upin_Bot2011_COI	ADL-A04	1	BD29200_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	CTTTAATTACAGCGTTTTTATTGTTGT	138.47562
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29201_Cjap_Sim2018_COI	ADL-A03	1	BD29201_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29201_Esin_And2018_Cyb	ADL-A03	1	BD29201_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A03	Cytb	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	ACCCCTCCTCATATCCAACCA	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29201_Mgig_Bot2012_COI	ADL-A03	1	BD29201_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	TCTTATTCGTTGGAGACTTTATAACCCT	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29201_Mjap_Gib2022_COI	ADL-A03	1	BD29201_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	AATTTAATTGTGACTAGGCATGGTCTG	138.47409
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.712	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29202_Cmae_Bot2011_COI	ADL-A02	1	BD29202_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	ATGAACAGTCTATCCTCCTTTAG	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29202_Hsan_Wil2021_COI	ADL-A02	1	BD29202_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	CCTGGGCCGGTATAGTAGGT	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29202_Mros_Gib2022_COI	ADL-A02	1	BD29202_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29202_Msal_Bot2012_COI	ADL-A02	1	BD29202_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	GYTAGTTCCRATGATGTTAGCTG	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29202_Mstr_Wil2021_COI	ADL-A02	1	BD29202_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	GTAGTGGTAACTACTCATGCTTTA	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29202_Pvir_Dia2013_COI	ADL-A02	1	BD29202_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	CTTAGTGGCATTAATTCGDAATCC	138.47521
BD29202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78407	WGS84	BD29202	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29202_Rhar_Sim2018_COI	ADL-A02	1	BD29202_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29202	Sample	BD29202	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A02	COI	45	BD29202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A02	CTGCTGCTATTGCTCATG	138.47521
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29209_Cjap_Sim2018_COI	THE-A35	1	BD29209_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29209_Esin_And2018_Cyb	THE-A35	1	BD29209_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A35	Cytb	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	ACCCCTCCTCATATCCAACCA	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29209_Mgig_Bot2012_COI	THE-A35	1	BD29209_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	TCTTATTCGTTGGAGACTTTATAACCCT	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29209_Mjap_Gib2022_COI	THE-A35	1	BD29209_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	AATTTAATTGTGACTAGGCATGGTCTG	133.64129
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29211_Aamu_Bax2006_COI	THE-A33	1	BD29211_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	GCACAACCGGGATCTTTACTTC	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29211_Pcan_Bot2011_IGS	THE-A33	1	BD29211_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A33	IGS	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	CGTAATCCTCAGTACTGGCTA	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29211_Pper_Dia2013_COI	THE-A33	1	BD29211_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	CTTAGTGGCATTAATTCGDAATCC	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29211_Sspa_Oph2007_COI	THE-A33	1	BD29211_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	GAAGGCCTTCCGATGATC	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29211_Upin_Bot2011_COI	THE-A33	1	BD29211_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	CTTTAATTACAGCGTTTTTATTGTTGT	133.63854
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29212_Cjap_Sim2018_COI	THE-A32	1	BD29212_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29212_Esin_And2018_Cyb	THE-A32	1	BD29212_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A32	Cytb	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	ACCCCTCCTCATATCCAACCA	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29212_Mgig_Bot2012_COI	THE-A32	1	BD29212_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	TCTTATTCGTTGGAGACTTTATAACCCT	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29212_Mjap_Gib2022_COI	THE-A32	1	BD29212_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	AATTTAATTGTGACTAGGCATGGTCTG	133.63713
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29213_Cmae_Bot2011_COI	THE-A31	1	BD29213_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	ATGAACAGTCTATCCTCCTTTAG	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29213_Hsan_Wil2021_COI	THE-A31	1	BD29213_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	CCTGGGCCGGTATAGTAGGT	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29213_Mros_Gib2022_COI	THE-A31	1	BD29213_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29213_Msal_Bot2012_COI	THE-A31	1	BD29213_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	GYTAGTTCCRATGATGTTAGCTG	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29213_Mstr_Wil2021_COI	THE-A31	1	BD29213_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	GTAGTGGTAACTACTCATGCTTTA	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29213_Pvir_Dia2013_COI	THE-A31	1	BD29213_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	CTTAGTGGCATTAATTCGDAATCC	133.63489
BD29213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14682	WGS84	BD29213	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29213_Rhar_Sim2018_COI	THE-A31	1	BD29213_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29213	Sample	BD29213	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A31	COI	45	BD29213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A31	CTGCTGCTATTGCTCATG	133.63489
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29215_Aamu_Bax2006_COI	THE-A29	1	BD29215_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	GCACAACCGGGATCTTTACTTC	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29215_Pcan_Bot2011_IGS	THE-A29	1	BD29215_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A29	IGS	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	CGTAATCCTCAGTACTGGCTA	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29215_Pper_Dia2013_COI	THE-A29	1	BD29215_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	CTTAGTGGCATTAATTCGDAATCC	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29215_Sspa_Oph2007_COI	THE-A29	1	BD29215_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	GAAGGCCTTCCGATGATC	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29215_Upin_Bot2011_COI	THE-A29	1	BD29215_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	CTTTAATTACAGCGTTTTTATTGTTGT	133.63786
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29216_Cjap_Sim2018_COI	THE-A28	1	BD29216_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29216_Esin_And2018_Cyb	THE-A28	1	BD29216_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A28	Cytb	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	ACCCCTCCTCATATCCAACCA	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29216_Mgig_Bot2012_COI	THE-A28	1	BD29216_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	TCTTATTCGTTGGAGACTTTATAACCCT	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29216_Mjap_Gib2022_COI	THE-A28	1	BD29216_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	AATTTAATTGTGACTAGGCATGGTCTG	133.63866
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29217_Cmae_Bot2011_COI	THE-A27	1	BD29217_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	ATGAACAGTCTATCCTCCTTTAG	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29217_Hsan_Wil2021_COI	THE-A27	1	BD29217_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	CCTGGGCCGGTATAGTAGGT	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29217_Mros_Gib2022_COI	THE-A27	1	BD29217_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29217_Msal_Bot2012_COI	THE-A27	1	BD29217_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	GYTAGTTCCRATGATGTTAGCTG	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29217_Mstr_Wil2021_COI	THE-A27	1	BD29217_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	GTAGTGGTAACTACTCATGCTTTA	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29217_Pvir_Dia2013_COI	THE-A27	1	BD29217_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	CTTAGTGGCATTAATTCGDAATCC	133.64061
BD29217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14685	WGS84	BD29217	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29217_Rhar_Sim2018_COI	THE-A27	1	BD29217_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29217	Sample	BD29217	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A27	COI	45	BD29217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A27	CTGCTGCTATTGCTCATG	133.64061
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29219_Aamu_Bax2006_COI	THE-A25	1	BD29219_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	GCACAACCGGGATCTTTACTTC	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29219_Pcan_Bot2011_IGS	THE-A25	1	BD29219_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A25	IGS	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	CGTAATCCTCAGTACTGGCTA	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29219_Pper_Dia2013_COI	THE-A25	1	BD29219_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	CTTAGTGGCATTAATTCGDAATCC	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29219_Sspa_Oph2007_COI	THE-A25	1	BD29219_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	GAAGGCCTTCCGATGATC	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29219_Upin_Bot2011_COI	THE-A25	1	BD29219_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	CTTTAATTACAGCGTTTTTATTGTTGT	133.64001
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29220_Cmae_Bot2011_COI	THE-A24	1	BD29220_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	ATGAACAGTCTATCCTCCTTTAG	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29220_Hsan_Wil2021_COI	THE-A24	1	BD29220_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	CCTGGGCCGGTATAGTAGGT	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29220_Mros_Gib2022_COI	THE-A24	1	BD29220_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29220_Msal_Bot2012_COI	THE-A24	1	BD29220_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	GYTAGTTCCRATGATGTTAGCTG	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29220_Mstr_Wil2021_COI	THE-A24	1	BD29220_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	GTAGTGGTAACTACTCATGCTTTA	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29220_Pvir_Dia2013_COI	THE-A24	1	BD29220_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	CTTAGTGGCATTAATTCGDAATCC	133.63854
BD29220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14777	WGS84	BD29220	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29220_Rhar_Sim2018_COI	THE-A24	1	BD29220_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29220	Sample	BD29220	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A24	COI	45	BD29220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A24	CTGCTGCTATTGCTCATG	133.63854
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29222_Aamu_Bax2006_COI	THE-A22	1	BD29222_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	GCACAACCGGGATCTTTACTTC	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29222_Pcan_Bot2011_IGS	THE-A22	1	BD29222_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A22	IGS	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	CGTAATCCTCAGTACTGGCTA	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29222_Pper_Dia2013_COI	THE-A22	1	BD29222_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	CTTAGTGGCATTAATTCGDAATCC	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29222_Sspa_Oph2007_COI	THE-A22	1	BD29222_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	GAAGGCCTTCCGATGATC	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29222_Upin_Bot2011_COI	THE-A22	1	BD29222_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	CTTTAATTACAGCGTTTTTATTGTTGT	133.63571
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29223_Cjap_Sim2018_COI	THE-A21	1	BD29223_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29223_Esin_And2018_Cyb	THE-A21	1	BD29223_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A21	Cytb	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	ACCCCTCCTCATATCCAACCA	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29223_Mgig_Bot2012_COI	THE-A21	1	BD29223_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	TCTTATTCGTTGGAGACTTTATAACCCT	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29223_Mjap_Gib2022_COI	THE-A21	1	BD29223_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	AATTTAATTGTGACTAGGCATGGTCTG	133.63495
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29224_Cmae_Bot2011_COI	THE-A20	1	BD29224_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	ATGAACAGTCTATCCTCCTTTAG	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29224_Hsan_Wil2021_COI	THE-A20	1	BD29224_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	CCTGGGCCGGTATAGTAGGT	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29224_Mros_Gib2022_COI	THE-A20	1	BD29224_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29224_Msal_Bot2012_COI	THE-A20	1	BD29224_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	GYTAGTTCCRATGATGTTAGCTG	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29224_Mstr_Wil2021_COI	THE-A20	1	BD29224_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	GTAGTGGTAACTACTCATGCTTTA	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29224_Pvir_Dia2013_COI	THE-A20	1	BD29224_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	CTTAGTGGCATTAATTCGDAATCC	133.63631
BD29224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14919	WGS84	BD29224	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29224_Rhar_Sim2018_COI	THE-A20	1	BD29224_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29224	Sample	BD29224	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A20	COI	45	BD29224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A20	CTGCTGCTATTGCTCATG	133.63631
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29226_Aamu_Bax2006_COI	THE-A18	1	BD29226_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	GCACAACCGGGATCTTTACTTC	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29226_Pcan_Bot2011_IGS	THE-A18	1	BD29226_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A18	IGS	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	CGTAATCCTCAGTACTGGCTA	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29226_Pper_Dia2013_COI	THE-A18	1	BD29226_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	CTTAGTGGCATTAATTCGDAATCC	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29226_Sspa_Oph2007_COI	THE-A18	1	BD29226_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	GAAGGCCTTCCGATGATC	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29226_Upin_Bot2011_COI	THE-A18	1	BD29226_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	CTTTAATTACAGCGTTTTTATTGTTGT	133.6378
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29227_Cjap_Sim2018_COI	THE-A17	1	BD29227_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29227_Esin_And2018_Cyb	THE-A17	1	BD29227_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A17	Cytb	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	ACCCCTCCTCATATCCAACCA	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29227_Mgig_Bot2012_COI	THE-A17	1	BD29227_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	TCTTATTCGTTGGAGACTTTATAACCCT	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29227_Mjap_Gib2022_COI	THE-A17	1	BD29227_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	AATTTAATTGTGACTAGGCATGGTCTG	133.63938
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29228_Cmae_Bot2011_COI	THE-A16	1	BD29228_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	ATGAACAGTCTATCCTCCTTTAG	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29228_Hsan_Wil2021_COI	THE-A16	1	BD29228_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	CCTGGGCCGGTATAGTAGGT	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29228_Mros_Gib2022_COI	THE-A16	1	BD29228_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29228_Msal_Bot2012_COI	THE-A16	1	BD29228_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	GYTAGTTCCRATGATGTTAGCTG	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29228_Mstr_Wil2021_COI	THE-A16	1	BD29228_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	GTAGTGGTAACTACTCATGCTTTA	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29228_Pvir_Dia2013_COI	THE-A16	1	BD29228_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	CTTAGTGGCATTAATTCGDAATCC	133.64079
BD29228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15218	WGS84	BD29228	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29228_Rhar_Sim2018_COI	THE-A16	1	BD29228_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29228	Sample	BD29228	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A16	COI	45	BD29228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A16	CTGCTGCTATTGCTCATG	133.64079
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29230_Cjap_Sim2018_COI	THE-A14	1	BD29230_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29230_Esin_And2018_Cyb	THE-A14	1	BD29230_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A14	Cytb	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	ACCCCTCCTCATATCCAACCA	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29230_Mgig_Bot2012_COI	THE-A14	1	BD29230_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	TCTTATTCGTTGGAGACTTTATAACCCT	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29230_Mjap_Gib2022_COI	THE-A14	1	BD29230_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	AATTTAATTGTGACTAGGCATGGTCTG	133.63852
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29231_Cmae_Bot2011_COI	THE-A13	1	BD29231_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	ATGAACAGTCTATCCTCCTTTAG	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29231_Hsan_Wil2021_COI	THE-A13	1	BD29231_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	CCTGGGCCGGTATAGTAGGT	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29231_Mros_Gib2022_COI	THE-A13	1	BD29231_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29231_Msal_Bot2012_COI	THE-A13	1	BD29231_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	GYTAGTTCCRATGATGTTAGCTG	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29231_Mstr_Wil2021_COI	THE-A13	1	BD29231_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	GTAGTGGTAACTACTCATGCTTTA	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29231_Pvir_Dia2013_COI	THE-A13	1	BD29231_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	CTTAGTGGCATTAATTCGDAATCC	133.63711
BD29231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15237	WGS84	BD29231	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29231_Rhar_Sim2018_COI	THE-A13	1	BD29231_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29231	Sample	BD29231	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A13	COI	45	BD29231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A13	CTGCTGCTATTGCTCATG	133.63711
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29233_Aamu_Bax2006_COI	THE-A12	1	BD29233_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	GCACAACCGGGATCTTTACTTC	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29233_Pcan_Bot2011_IGS	THE-A12	1	BD29233_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A12	IGS	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	CGTAATCCTCAGTACTGGCTA	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29233_Pper_Dia2013_COI	THE-A12	1	BD29233_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	CTTAGTGGCATTAATTCGDAATCC	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29233_Sspa_Oph2007_COI	THE-A12	1	BD29233_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	GAAGGCCTTCCGATGATC	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29233_Upin_Bot2011_COI	THE-A12	1	BD29233_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	CTTTAATTACAGCGTTTTTATTGTTGT	133.63562
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29234_Cjap_Sim2018_COI	THE-A10	1	BD29234_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29234_Esin_And2018_Cyb	THE-A10	1	BD29234_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A10	Cytb	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	ACCCCTCCTCATATCCAACCA	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29234_Mgig_Bot2012_COI	THE-A10	1	BD29234_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	TCTTATTCGTTGGAGACTTTATAACCCT	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29234_Mjap_Gib2022_COI	THE-A10	1	BD29234_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	AATTTAATTGTGACTAGGCATGGTCTG	133.63622
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29235_Cmae_Bot2011_COI	THE-A09	1	BD29235_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	ATGAACAGTCTATCCTCCTTTAG	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29235_Hsan_Wil2021_COI	THE-A09	1	BD29235_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	CCTGGGCCGGTATAGTAGGT	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29235_Mros_Gib2022_COI	THE-A09	1	BD29235_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29235_Msal_Bot2012_COI	THE-A09	1	BD29235_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	GYTAGTTCCRATGATGTTAGCTG	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29235_Mstr_Wil2021_COI	THE-A09	1	BD29235_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	GTAGTGGTAACTACTCATGCTTTA	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29235_Pvir_Dia2013_COI	THE-A09	1	BD29235_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	CTTAGTGGCATTAATTCGDAATCC	133.63786
BD29235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15135	WGS84	BD29235	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29235_Rhar_Sim2018_COI	THE-A09	1	BD29235_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29235	Sample	BD29235	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A09	COI	45	BD29235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A09	CTGCTGCTATTGCTCATG	133.63786
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29237_Aamu_Bax2006_COI	THE-A07	1	BD29237_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	GCACAACCGGGATCTTTACTTC	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29237_Pcan_Bot2011_IGS	THE-A07	1	BD29237_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A07	IGS	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	CGTAATCCTCAGTACTGGCTA	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29237_Pper_Dia2013_COI	THE-A07	1	BD29237_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	CTTAGTGGCATTAATTCGDAATCC	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29237_Sspa_Oph2007_COI	THE-A07	1	BD29237_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	GAAGGCCTTCCGATGATC	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29237_Upin_Bot2011_COI	THE-A07	1	BD29237_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	CTTTAATTACAGCGTTTTTATTGTTGT	133.64068
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29238_Cjap_Sim2018_COI	THE-A06	1	BD29238_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29238_Esin_And2018_Cyb	THE-A06	1	BD29238_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A06	Cytb	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	ACCCCTCCTCATATCCAACCA	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29238_Mgig_Bot2012_COI	THE-A06	1	BD29238_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	TCTTATTCGTTGGAGACTTTATAACCCT	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29238_Mjap_Gib2022_COI	THE-A06	1	BD29238_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	AATTTAATTGTGACTAGGCATGGTCTG	133.64205
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29239_Cmae_Bot2011_COI	THE-A05	1	BD29239_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	ATGAACAGTCTATCCTCCTTTAG	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29239_Hsan_Wil2021_COI	THE-A05	1	BD29239_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	CCTGGGCCGGTATAGTAGGT	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29239_Mros_Gib2022_COI	THE-A05	1	BD29239_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29239_Msal_Bot2012_COI	THE-A05	1	BD29239_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	GYTAGTTCCRATGATGTTAGCTG	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29239_Mstr_Wil2021_COI	THE-A05	1	BD29239_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	GTAGTGGTAACTACTCATGCTTTA	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29239_Pvir_Dia2013_COI	THE-A05	1	BD29239_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	CTTAGTGGCATTAATTCGDAATCC	133.64142
BD29239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15044	WGS84	BD29239	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29239_Rhar_Sim2018_COI	THE-A05	1	BD29239_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29239	Sample	BD29239	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A05	COI	45	BD29239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A05	CTGCTGCTATTGCTCATG	133.64142
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29240_Aamu_Bax2006_COI	THE-A04	1	BD29240_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	GCACAACCGGGATCTTTACTTC	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29240_Pcan_Bot2011_IGS	THE-A04	1	BD29240_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A04	IGS	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	CGTAATCCTCAGTACTGGCTA	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29240_Pper_Dia2013_COI	THE-A04	1	BD29240_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	CTTAGTGGCATTAATTCGDAATCC	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29240_Sspa_Oph2007_COI	THE-A04	1	BD29240_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	GAAGGCCTTCCGATGATC	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29240_Upin_Bot2011_COI	THE-A04	1	BD29240_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	CTTTAATTACAGCGTTTTTATTGTTGT	133.63986
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29241_Cjap_Sim2018_COI	THE-A03	1	BD29241_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29241_Esin_And2018_Cyb	THE-A03	1	BD29241_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A03	Cytb	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	ACCCCTCCTCATATCCAACCA	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29241_Mgig_Bot2012_COI	THE-A03	1	BD29241_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	TCTTATTCGTTGGAGACTTTATAACCCT	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29241_Mjap_Gib2022_COI	THE-A03	1	BD29241_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	AATTTAATTGTGACTAGGCATGGTCTG	133.63825
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29242_Cmae_Bot2011_COI	THE-A02	1	BD29242_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	ATGAACAGTCTATCCTCCTTTAG	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29242_Hsan_Wil2021_COI	THE-A02	1	BD29242_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	CCTGGGCCGGTATAGTAGGT	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29242_Mros_Gib2022_COI	THE-A02	1	BD29242_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29242_Msal_Bot2012_COI	THE-A02	1	BD29242_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	GYTAGTTCCRATGATGTTAGCTG	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29242_Mstr_Wil2021_COI	THE-A02	1	BD29242_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	GTAGTGGTAACTACTCATGCTTTA	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29242_Pvir_Dia2013_COI	THE-A02	1	BD29242_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	CTTAGTGGCATTAATTCGDAATCC	133.6372
BD29242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15051	WGS84	BD29242	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29242_Rhar_Sim2018_COI	THE-A02	1	BD29242_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29242	Sample	BD29242	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A02	COI	45	BD29242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A02	CTGCTGCTATTGCTCATG	133.6372
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29244_Aamu_Bax2006_COI	PLO-A40	1	BD29244_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	GCACAACCGGGATCTTTACTTC	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29244_Pcan_Bot2011_IGS	PLO-A40	1	BD29244_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A40	IGS	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	CGTAATCCTCAGTACTGGCTA	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29244_Pper_Dia2013_COI	PLO-A40	1	BD29244_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	CTTAGTGGCATTAATTCGDAATCC	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.531	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29244_Sspa_Oph2007_COI	PLO-A40	1	BD29244_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	GAAGGCCTTCCGATGATC	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29244_Upin_Bot2011_COI	PLO-A40	1	BD29244_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	CTTTAATTACAGCGTTTTTATTGTTGT	135.87311
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29245_Cjap_Sim2018_COI	PLO-A39	1	BD29245_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29245_Esin_And2018_Cyb	PLO-A39	1	BD29245_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A39	Cytb	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	ACCCCTCCTCATATCCAACCA	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29245_Mgig_Bot2012_COI	PLO-A39	1	BD29245_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	TCTTATTCGTTGGAGACTTTATAACCCT	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29245_Mjap_Gib2022_COI	PLO-A39	1	BD29245_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	AATTTAATTGTGACTAGGCATGGTCTG	135.8719
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29246_Cmae_Bot2011_COI	PLO-A38	1	BD29246_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	ATGAACAGTCTATCCTCCTTTAG	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29246_Hsan_Wil2021_COI	PLO-A38	1	BD29246_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	CCTGGGCCGGTATAGTAGGT	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29246_Mros_Gib2022_COI	PLO-A38	1	BD29246_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29246_Msal_Bot2012_COI	PLO-A38	1	BD29246_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	GYTAGTTCCRATGATGTTAGCTG	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29246_Mstr_Wil2021_COI	PLO-A38	1	BD29246_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	GTAGTGGTAACTACTCATGCTTTA	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29246_Pvir_Dia2013_COI	PLO-A38	1	BD29246_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	CTTAGTGGCATTAATTCGDAATCC	135.87366
BD29246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74207	WGS84	BD29246	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29246_Rhar_Sim2018_COI	PLO-A38	1	BD29246_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29246	Sample	BD29246	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A38	COI	45	BD29246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A38	CTGCTGCTATTGCTCATG	135.87366
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29248_Aamu_Bax2006_COI	PLO-A36	1	BD29248_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	GCACAACCGGGATCTTTACTTC	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29248_Pcan_Bot2011_IGS	PLO-A36	1	BD29248_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A36	IGS	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	CGTAATCCTCAGTACTGGCTA	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29248_Pper_Dia2013_COI	PLO-A36	1	BD29248_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	CTTAGTGGCATTAATTCGDAATCC	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29248_Sspa_Oph2007_COI	PLO-A36	1	BD29248_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	GAAGGCCTTCCGATGATC	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29248_Upin_Bot2011_COI	PLO-A36	1	BD29248_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	CTTTAATTACAGCGTTTTTATTGTTGT	135.87696
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29249_Cjap_Sim2018_COI	PLO-A35	1	BD29249_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29249_Esin_And2018_Cyb	PLO-A35	1	BD29249_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A35	Cytb	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	ACCCCTCCTCATATCCAACCA	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29249_Mgig_Bot2012_COI	PLO-A35	1	BD29249_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	TCTTATTCGTTGGAGACTTTATAACCCT	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29249_Mjap_Gib2022_COI	PLO-A35	1	BD29249_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	AATTTAATTGTGACTAGGCATGGTCTG	135.87281
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29251_Aamu_Bax2006_COI	PLO-A33	1	BD29251_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	GCACAACCGGGATCTTTACTTC	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29251_Pcan_Bot2011_IGS	PLO-A33	1	BD29251_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A33	IGS	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	CGTAATCCTCAGTACTGGCTA	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29251_Pper_Dia2013_COI	PLO-A33	1	BD29251_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	CTTAGTGGCATTAATTCGDAATCC	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.442	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29251_Sspa_Oph2007_COI	PLO-A33	1	BD29251_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	GAAGGCCTTCCGATGATC	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29251_Upin_Bot2011_COI	PLO-A33	1	BD29251_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	CTTTAATTACAGCGTTTTTATTGTTGT	135.87175
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29252_Cjap_Sim2018_COI	PLO-A32	1	BD29252_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29252_Esin_And2018_Cyb	PLO-A32	1	BD29252_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A32	Cytb	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	ACCCCTCCTCATATCCAACCA	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29252_Mgig_Bot2012_COI	PLO-A32	1	BD29252_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	TCTTATTCGTTGGAGACTTTATAACCCT	135.87354
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29253_Cmae_Bot2011_COI	PLO-A31	1	BD29253_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	ATGAACAGTCTATCCTCCTTTAG	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29253_Hsan_Wil2021_COI	PLO-A31	1	BD29253_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	CCTGGGCCGGTATAGTAGGT	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29253_Mros_Gib2022_COI	PLO-A31	1	BD29253_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29253_Msal_Bot2012_COI	PLO-A31	1	BD29253_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	GYTAGTTCCRATGATGTTAGCTG	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29253_Mstr_Wil2021_COI	PLO-A31	1	BD29253_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	GTAGTGGTAACTACTCATGCTTTA	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29253_Pvir_Dia2013_COI	PLO-A31	1	BD29253_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	CTTAGTGGCATTAATTCGDAATCC	135.87589
BD29253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71464	WGS84	BD29253	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29253_Rhar_Sim2018_COI	PLO-A31	1	BD29253_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29253	Sample	BD29253	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A31	COI	45	BD29253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A31	CTGCTGCTATTGCTCATG	135.87589
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29255_Aamu_Bax2006_COI	PLO-A29	1	BD29255_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	GCACAACCGGGATCTTTACTTC	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29255_Pcan_Bot2011_IGS	PLO-A29	1	BD29255_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A29	IGS	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	CGTAATCCTCAGTACTGGCTA	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29255_Pper_Dia2013_COI	PLO-A29	1	BD29255_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	CTTAGTGGCATTAATTCGDAATCC	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.005	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29255_Sspa_Oph2007_COI	PLO-A29	1	BD29255_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	GAAGGCCTTCCGATGATC	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29255_Upin_Bot2011_COI	PLO-A29	1	BD29255_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	CTTTAATTACAGCGTTTTTATTGTTGT	135.87799
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29256_Cjap_Sim2018_COI	PLO-A28	1	BD29256_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29256_Esin_And2018_Cyb	PLO-A28	1	BD29256_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A28	Cytb	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	ACCCCTCCTCATATCCAACCA	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29256_Mgig_Bot2012_COI	PLO-A28	1	BD29256_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	TCTTATTCGTTGGAGACTTTATAACCCT	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29256_Mjap_Gib2022_COI	PLO-A28	1	BD29256_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	AATTTAATTGTGACTAGGCATGGTCTG	135.87249
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29257_Cmae_Bot2011_COI	PLO-A27	1	BD29257_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	ATGAACAGTCTATCCTCCTTTAG	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29257_Hsan_Wil2021_COI	PLO-A27	1	BD29257_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	CCTGGGCCGGTATAGTAGGT	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29257_Mros_Gib2022_COI	PLO-A27	1	BD29257_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29257_Msal_Bot2012_COI	PLO-A27	1	BD29257_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	GYTAGTTCCRATGATGTTAGCTG	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29257_Mstr_Wil2021_COI	PLO-A27	1	BD29257_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	GTAGTGGTAACTACTCATGCTTTA	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29257_Pvir_Dia2013_COI	PLO-A27	1	BD29257_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	CTTAGTGGCATTAATTCGDAATCC	135.87467
BD29257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71315	WGS84	BD29257	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29257_Rhar_Sim2018_COI	PLO-A27	1	BD29257_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29257	Sample	BD29257	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A27	COI	45	BD29257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A27	CTGCTGCTATTGCTCATG	135.87467
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29259_Aamu_Bax2006_COI	PLO-A25	1	BD29259_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	GCACAACCGGGATCTTTACTTC	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29259_Pcan_Bot2011_IGS	PLO-A25	1	BD29259_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A25	IGS	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	CGTAATCCTCAGTACTGGCTA	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29259_Pper_Dia2013_COI	PLO-A25	1	BD29259_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	CTTAGTGGCATTAATTCGDAATCC	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29259_Sspa_Oph2007_COI	PLO-A25	1	BD29259_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	GAAGGCCTTCCGATGATC	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29259_Upin_Bot2011_COI	PLO-A25	1	BD29259_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	CTTTAATTACAGCGTTTTTATTGTTGT	135.87256
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29260_Cmae_Bot2011_COI	PLO-A22	1	BD29260_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	ATGAACAGTCTATCCTCCTTTAG	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29260_Hsan_Wil2021_COI	PLO-A22	1	BD29260_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	CCTGGGCCGGTATAGTAGGT	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29260_Mros_Gib2022_COI	PLO-A22	1	BD29260_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29260_Msal_Bot2012_COI	PLO-A22	1	BD29260_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	GYTAGTTCCRATGATGTTAGCTG	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29260_Mstr_Wil2021_COI	PLO-A22	1	BD29260_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	GTAGTGGTAACTACTCATGCTTTA	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29260_Pvir_Dia2013_COI	PLO-A22	1	BD29260_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	CTTAGTGGCATTAATTCGDAATCC	135.86801
BD29260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70992	WGS84	BD29260	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29260_Rhar_Sim2018_COI	PLO-A22	1	BD29260_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29260	Sample	BD29260	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A22	COI	45	BD29260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A22	CTGCTGCTATTGCTCATG	135.86801
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29262_Aamu_Bax2006_COI	PLO-A23	1	BD29262_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	GCACAACCGGGATCTTTACTTC	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29262_Pcan_Bot2011_IGS	PLO-A23	1	BD29262_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A23	IGS	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	CGTAATCCTCAGTACTGGCTA	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29262_Pper_Dia2013_COI	PLO-A23	1	BD29262_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	CTTAGTGGCATTAATTCGDAATCC	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29262_Sspa_Oph2007_COI	PLO-A23	1	BD29262_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	GAAGGCCTTCCGATGATC	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29262_Upin_Bot2011_COI	PLO-A23	1	BD29262_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	CTTTAATTACAGCGTTTTTATTGTTGT	135.87022
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29264_Cmae_Bot2011_COI	PLO-A21	1	BD29264_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	ATGAACAGTCTATCCTCCTTTAG	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29264_Hsan_Wil2021_COI	PLO-A21	1	BD29264_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	CCTGGGCCGGTATAGTAGGT	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29264_Mros_Gib2022_COI	PLO-A21	1	BD29264_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29264_Msal_Bot2012_COI	PLO-A21	1	BD29264_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	GYTAGTTCCRATGATGTTAGCTG	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29264_Mstr_Wil2021_COI	PLO-A21	1	BD29264_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	GTAGTGGTAACTACTCATGCTTTA	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29264_Pvir_Dia2013_COI	PLO-A21	1	BD29264_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	CTTAGTGGCATTAATTCGDAATCC	135.86904
BD29264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71165	WGS84	BD29264	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29264_Rhar_Sim2018_COI	PLO-A21	1	BD29264_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29264	Sample	BD29264	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A21	COI	45	BD29264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A21	CTGCTGCTATTGCTCATG	135.86904
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29266_Aamu_Bax2006_COI	PLO-A19	1	BD29266_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	GCACAACCGGGATCTTTACTTC	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29266_Pcan_Bot2011_IGS	PLO-A19	1	BD29266_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A19	IGS	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	CGTAATCCTCAGTACTGGCTA	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29266_Pper_Dia2013_COI	PLO-A19	1	BD29266_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	CTTAGTGGCATTAATTCGDAATCC	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29266_Sspa_Oph2007_COI	PLO-A19	1	BD29266_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	GAAGGCCTTCCGATGATC	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29266_Upin_Bot2011_COI	PLO-A19	1	BD29266_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	CTTTAATTACAGCGTTTTTATTGTTGT	135.86574
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29267_Cjap_Sim2018_COI	PLO-A18	1	BD29267_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29267_Esin_And2018_Cyb	PLO-A18	1	BD29267_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A18	Cytb	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	ACCCCTCCTCATATCCAACCA	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29267_Mgig_Bot2012_COI	PLO-A18	1	BD29267_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	TCTTATTCGTTGGAGACTTTATAACCCT	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29267_Mjap_Gib2022_COI	PLO-A18	1	BD29267_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	AATTTAATTGTGACTAGGCATGGTCTG	135.86819
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29268_Cmae_Bot2011_COI	PLO-A17	1	BD29268_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	ATGAACAGTCTATCCTCCTTTAG	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29268_Hsan_Wil2021_COI	PLO-A17	1	BD29268_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	CCTGGGCCGGTATAGTAGGT	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29268_Mros_Gib2022_COI	PLO-A17	1	BD29268_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29268_Msal_Bot2012_COI	PLO-A17	1	BD29268_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	GYTAGTTCCRATGATGTTAGCTG	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29268_Mstr_Wil2021_COI	PLO-A17	1	BD29268_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	GTAGTGGTAACTACTCATGCTTTA	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29268_Pvir_Dia2013_COI	PLO-A17	1	BD29268_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	CTTAGTGGCATTAATTCGDAATCC	135.8648
BD29268	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71473	WGS84	BD29268	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29268_Rhar_Sim2018_COI	PLO-A17	1	BD29268_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29268	Sample	BD29268	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A17	COI	45	BD29268	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A17	CTGCTGCTATTGCTCATG	135.8648
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29270_Cjap_Sim2018_COI	PLO-A15	1	BD29270_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29270_Esin_And2018_Cyb	PLO-A15	1	BD29270_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A15	Cytb	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	ACCCCTCCTCATATCCAACCA	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29270_Mgig_Bot2012_COI	PLO-A15	1	BD29270_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	TCTTATTCGTTGGAGACTTTATAACCCT	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29270_Mjap_Gib2022_COI	PLO-A15	1	BD29270_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	AATTTAATTGTGACTAGGCATGGTCTG	135.86365
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29271_Cmae_Bot2011_COI	PLO-A14	1	BD29271_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	ATGAACAGTCTATCCTCCTTTAG	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29271_Hsan_Wil2021_COI	PLO-A14	1	BD29271_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	CCTGGGCCGGTATAGTAGGT	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29271_Mros_Gib2022_COI	PLO-A14	1	BD29271_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29271_Msal_Bot2012_COI	PLO-A14	1	BD29271_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	GYTAGTTCCRATGATGTTAGCTG	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29271_Mstr_Wil2021_COI	PLO-A14	1	BD29271_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	GTAGTGGTAACTACTCATGCTTTA	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29271_Pvir_Dia2013_COI	PLO-A14	1	BD29271_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	CTTAGTGGCATTAATTCGDAATCC	135.86587
BD29271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71635	WGS84	BD29271	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29271_Rhar_Sim2018_COI	PLO-A14	1	BD29271_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29271	Sample	BD29271	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A14	COI	45	BD29271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A14	CTGCTGCTATTGCTCATG	135.86587
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29273_Aamu_Bax2006_COI	PLO-A12	1	BD29273_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	GCACAACCGGGATCTTTACTTC	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29273_Pcan_Bot2011_IGS	PLO-A12	1	BD29273_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A12	IGS	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	CGTAATCCTCAGTACTGGCTA	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29273_Pper_Dia2013_COI	PLO-A12	1	BD29273_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	CTTAGTGGCATTAATTCGDAATCC	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.371	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29273_Sspa_Oph2007_COI	PLO-A12	1	BD29273_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	GAAGGCCTTCCGATGATC	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29273_Upin_Bot2011_COI	PLO-A12	1	BD29273_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	CTTTAATTACAGCGTTTTTATTGTTGT	135.86717
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29274_Cjap_Sim2018_COI	PLO-A11	1	BD29274_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29274_Esin_And2018_Cyb	PLO-A11	1	BD29274_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A11	Cytb	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	ACCCCTCCTCATATCCAACCA	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29274_Mgig_Bot2012_COI	PLO-A11	1	BD29274_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	TCTTATTCGTTGGAGACTTTATAACCCT	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29274_Mjap_Gib2022_COI	PLO-A11	1	BD29274_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	AATTTAATTGTGACTAGGCATGGTCTG	135.86498
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29275_Cmae_Bot2011_COI	PLO-A10	1	BD29275_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	ATGAACAGTCTATCCTCCTTTAG	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29275_Hsan_Wil2021_COI	PLO-A10	1	BD29275_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	CCTGGGCCGGTATAGTAGGT	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29275_Mros_Gib2022_COI	PLO-A10	1	BD29275_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29275_Msal_Bot2012_COI	PLO-A10	1	BD29275_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	GYTAGTTCCRATGATGTTAGCTG	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29275_Mstr_Wil2021_COI	PLO-A10	1	BD29275_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	GTAGTGGTAACTACTCATGCTTTA	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29275_Pvir_Dia2013_COI	PLO-A10	1	BD29275_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	CTTAGTGGCATTAATTCGDAATCC	135.8636
BD29275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71969	WGS84	BD29275	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29275_Rhar_Sim2018_COI	PLO-A10	1	BD29275_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29275	Sample	BD29275	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A10	COI	45	BD29275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A10	CTGCTGCTATTGCTCATG	135.8636
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29277_Aamu_Bax2006_COI	PLO-A08	1	BD29277_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	GCACAACCGGGATCTTTACTTC	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29277_Pcan_Bot2011_IGS	PLO-A08	1	BD29277_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A08	IGS	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	CGTAATCCTCAGTACTGGCTA	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29277_Pper_Dia2013_COI	PLO-A08	1	BD29277_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	CTTAGTGGCATTAATTCGDAATCC	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.544	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29277_Sspa_Oph2007_COI	PLO-A08	1	BD29277_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	GAAGGCCTTCCGATGATC	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29277_Upin_Bot2011_COI	PLO-A08	1	BD29277_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	CTTTAATTACAGCGTTTTTATTGTTGT	135.87218
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29278_Cjap_Sim2018_COI	PLO-A07	1	BD29278_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29278_Esin_And2018_Cyb	PLO-A07	1	BD29278_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A07	Cytb	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	ACCCCTCCTCATATCCAACCA	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29278_Mgig_Bot2012_COI	PLO-A07	1	BD29278_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	TCTTATTCGTTGGAGACTTTATAACCCT	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29278_Mjap_Gib2022_COI	PLO-A07	1	BD29278_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	AATTTAATTGTGACTAGGCATGGTCTG	135.8729
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29279_Cmae_Bot2011_COI	PLO-A06	1	BD29279_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	ATGAACAGTCTATCCTCCTTTAG	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29279_Hsan_Wil2021_COI	PLO-A06	1	BD29279_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	CCTGGGCCGGTATAGTAGGT	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29279_Mros_Gib2022_COI	PLO-A06	1	BD29279_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29279_Msal_Bot2012_COI	PLO-A06	1	BD29279_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	GYTAGTTCCRATGATGTTAGCTG	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29279_Mstr_Wil2021_COI	PLO-A06	1	BD29279_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	GTAGTGGTAACTACTCATGCTTTA	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29279_Pvir_Dia2013_COI	PLO-A06	1	BD29279_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	CTTAGTGGCATTAATTCGDAATCC	135.8739
BD29279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71781	WGS84	BD29279	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29279_Rhar_Sim2018_COI	PLO-A06	1	BD29279_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29279	Sample	BD29279	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A06	COI	45	BD29279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A06	CTGCTGCTATTGCTCATG	135.8739
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29280_Aamu_Bax2006_COI	PLO-A05	1	BD29280_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	GCACAACCGGGATCTTTACTTC	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29280_Pcan_Bot2011_IGS	PLO-A05	1	BD29280_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A05	IGS	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	CGTAATCCTCAGTACTGGCTA	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29280_Pper_Dia2013_COI	PLO-A05	1	BD29280_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	CTTAGTGGCATTAATTCGDAATCC	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.103	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29280_Sspa_Oph2007_COI	PLO-A05	1	BD29280_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	GAAGGCCTTCCGATGATC	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29280_Upin_Bot2011_COI	PLO-A05	1	BD29280_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	CTTTAATTACAGCGTTTTTATTGTTGT	135.87604
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29281_Cjap_Sim2018_COI	PLO-A04	1	BD29281_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29281_Esin_And2018_Cyb	PLO-A04	1	BD29281_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A04	Cytb	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	ACCCCTCCTCATATCCAACCA	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29281_Mgig_Bot2012_COI	PLO-A04	1	BD29281_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	TCTTATTCGTTGGAGACTTTATAACCCT	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29281_Mjap_Gib2022_COI	PLO-A04	1	BD29281_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	AATTTAATTGTGACTAGGCATGGTCTG	135.87711
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29282_Cmae_Bot2011_COI	PLO-A03	1	BD29282_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	ATGAACAGTCTATCCTCCTTTAG	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29282_Hsan_Wil2021_COI	PLO-A03	1	BD29282_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	CCTGGGCCGGTATAGTAGGT	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29282_Mros_Gib2022_COI	PLO-A03	1	BD29282_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29282_Msal_Bot2012_COI	PLO-A03	1	BD29282_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	GYTAGTTCCRATGATGTTAGCTG	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29282_Mstr_Wil2021_COI	PLO-A03	1	BD29282_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	GTAGTGGTAACTACTCATGCTTTA	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29282_Pvir_Dia2013_COI	PLO-A03	1	BD29282_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	CTTAGTGGCATTAATTCGDAATCC	135.87891
BD29282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71798	WGS84	BD29282	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29282_Rhar_Sim2018_COI	PLO-A03	1	BD29282_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29282	Sample	BD29282	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A03	COI	45	BD29282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A03	CTGCTGCTATTGCTCATG	135.87891
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29284_Aamu_Bax2006_COI	PLO-A01	1	BD29284_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	GCACAACCGGGATCTTTACTTC	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29284_Pcan_Bot2011_IGS	PLO-A01	1	BD29284_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A01	IGS	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	CGTAATCCTCAGTACTGGCTA	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29284_Pper_Dia2013_COI	PLO-A01	1	BD29284_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	CTTAGTGGCATTAATTCGDAATCC	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29284_Sspa_Oph2007_COI	PLO-A01	1	BD29284_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	GAAGGCCTTCCGATGATC	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29284_Upin_Bot2011_COI	PLO-A01	1	BD29284_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	CTTTAATTACAGCGTTTTTATTGTTGT	135.8807
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29285_Cjap_Sim2018_COI	POR-A35	1	BD29285_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29285_Esin_And2018_Cyb	POR-A35	1	BD29285_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A35	Cytb	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	ACCCCTCCTCATATCCAACCA	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29285_Mgig_Bot2012_COI	POR-A35	1	BD29285_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	TCTTATTCGTTGGAGACTTTATAACCCT	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29285_Mjap_Gib2022_COI	POR-A35	1	BD29285_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	AATTTAATTGTGACTAGGCATGGTCTG	141.61391
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29286_Cmae_Bot2011_COI	POR-A34	1	BD29286_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	ATGAACAGTCTATCCTCCTTTAG	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29286_Hsan_Wil2021_COI	POR-A34	1	BD29286_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	CCTGGGCCGGTATAGTAGGT	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.376	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29286_Mros_Gib2022_COI	POR-A34	1	BD29286_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Present	Sample	Mros_Gib2022_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29286_Msal_Bot2012_COI	POR-A34	1	BD29286_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	GYTAGTTCCRATGATGTTAGCTG	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29286_Mstr_Wil2021_COI	POR-A34	1	BD29286_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	GTAGTGGTAACTACTCATGCTTTA	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29286_Pvir_Dia2013_COI	POR-A34	1	BD29286_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	CTTAGTGGCATTAATTCGDAATCC	141.61249
BD29286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34484	WGS84	BD29286	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29286_Rhar_Sim2018_COI	POR-A34	1	BD29286_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29286	Sample	BD29286	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A34	COI	45	BD29286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A34	CTGCTGCTATTGCTCATG	141.61249
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29288_Aamu_Bax2006_COI	POR-A32	1	BD29288_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	GCACAACCGGGATCTTTACTTC	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29288_Pcan_Bot2011_IGS	POR-A32	1	BD29288_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A32	IGS	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	CGTAATCCTCAGTACTGGCTA	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29288_Pper_Dia2013_COI	POR-A32	1	BD29288_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	CTTAGTGGCATTAATTCGDAATCC	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29288_Sspa_Oph2007_COI	POR-A32	1	BD29288_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	GAAGGCCTTCCGATGATC	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.439	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29288_Upin_Bot2011_COI	POR-A32	1	BD29288_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Present	Sample	Upin_Bot2011_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	CTTTAATTACAGCGTTTTTATTGTTGT	141.62275
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29289_Cjap_Sim2018_COI	POR-A31	1	BD29289_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29289_Esin_And2018_Cyb	POR-A31	1	BD29289_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A31	Cytb	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	ACCCCTCCTCATATCCAACCA	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29289_Mgig_Bot2012_COI	POR-A31	1	BD29289_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	TCTTATTCGTTGGAGACTTTATAACCCT	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29289_Mjap_Gib2022_COI	POR-A31	1	BD29289_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	AATTTAATTGTGACTAGGCATGGTCTG	141.62149
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29291_Aamu_Bax2006_COI	POR-A29	1	BD29291_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	GCACAACCGGGATCTTTACTTC	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29291_Pcan_Bot2011_IGS	POR-A29	1	BD29291_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A29	IGS	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	CGTAATCCTCAGTACTGGCTA	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29291_Pper_Dia2013_COI	POR-A29	1	BD29291_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	CTTAGTGGCATTAATTCGDAATCC	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.926	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29291_Sspa_Oph2007_COI	POR-A29	1	BD29291_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	GAAGGCCTTCCGATGATC	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.217	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29291_Upin_Bot2011_COI	POR-A29	1	BD29291_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Present	Sample	Upin_Bot2011_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	CTTTAATTACAGCGTTTTTATTGTTGT	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29291_Vgib_Wil2025_COI	POR-A29	1	BD29291_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	CCCCTCTTTACAAGTTCTTTAGGGT	141.61874
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29292_Asen_Wil2025_COI	POR-A28	1	BD29292_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	GTTGTTGTTACAACACATGCTCTTTTG	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29292_Cjap_Sim2018_COI	POR-A28	1	BD29292_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29292_Esin_And2018_Cyb	POR-A28	1	BD29292_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A28	Cytb	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	ACCCCTCCTCATATCCAACCA	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29292_Mgig_Bot2012_COI	POR-A28	1	BD29292_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	TCTTATTCGTTGGAGACTTTATAACCCT	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29292_Mjap_Gib2022_COI	POR-A28	1	BD29292_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	AATTTAATTGTGACTAGGCATGGTCTG	141.61735
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29293_Cmae_Bot2011_COI	POR-A27	1	BD29293_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	ATGAACAGTCTATCCTCCTTTAG	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29293_Hsan_Wil2021_COI	POR-A27	1	BD29293_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	CCTGGGCCGGTATAGTAGGT	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29293_Mros_Gib2022_COI	POR-A27	1	BD29293_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29293_Msal_Bot2012_COI	POR-A27	1	BD29293_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	GYTAGTTCCRATGATGTTAGCTG	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29293_Mstr_Wil2021_COI	POR-A27	1	BD29293_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	GTAGTGGTAACTACTCATGCTTTA	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29293_Pvir_Dia2013_COI	POR-A27	1	BD29293_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	CTTAGTGGCATTAATTCGDAATCC	141.61596
BD29293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34558	WGS84	BD29293	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29293_Rhar_Sim2018_COI	POR-A27	1	BD29293_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29293	Sample	BD29293	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A27	COI	45	BD29293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A27	CTGCTGCTATTGCTCATG	141.61596
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29295_Aamu_Bax2006_COI	POR-A25	1	BD29295_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	GCACAACCGGGATCTTTACTTC	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29295_Pcan_Bot2011_IGS	POR-A25	1	BD29295_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A25	IGS	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	CGTAATCCTCAGTACTGGCTA	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29295_Pper_Dia2013_COI	POR-A25	1	BD29295_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	CTTAGTGGCATTAATTCGDAATCC	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.015	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29295_Sspa_Oph2007_COI	POR-A25	1	BD29295_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	GAAGGCCTTCCGATGATC	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.538	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29295_Upin_Bot2011_COI	POR-A25	1	BD29295_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Present	Sample	Upin_Bot2011_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	CTTTAATTACAGCGTTTTTATTGTTGT	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29295_Vgib_Wil2025_COI	POR-A25	1	BD29295_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	CCCCTCTTTACAAGTTCTTTAGGGT	141.61313
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29296_Asen_Wil2025_COI	POR-A24	1	BD29296_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	GTTGTTGTTACAACACATGCTCTTTTG	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29296_Cjap_Sim2018_COI	POR-A24	1	BD29296_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29296_Esin_And2018_Cyb	POR-A24	1	BD29296_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A24	Cytb	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	ACCCCTCCTCATATCCAACCA	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29296_Mgig_Bot2012_COI	POR-A24	1	BD29296_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	TCTTATTCGTTGGAGACTTTATAACCCT	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29296_Mjap_Gib2022_COI	POR-A24	1	BD29296_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	AATTTAATTGTGACTAGGCATGGTCTG	141.6222
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29297_Cmae_Bot2011_COI	POR-A23	1	BD29297_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	ATGAACAGTCTATCCTCCTTTAG	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29297_Hsan_Wil2021_COI	POR-A23	1	BD29297_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	CCTGGGCCGGTATAGTAGGT	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.657	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29297_Mros_Gib2022_COI	POR-A23	1	BD29297_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Present	Sample	Mros_Gib2022_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29297_Msal_Bot2012_COI	POR-A23	1	BD29297_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	GYTAGTTCCRATGATGTTAGCTG	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29297_Mstr_Wil2021_COI	POR-A23	1	BD29297_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	GTAGTGGTAACTACTCATGCTTTA	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29297_Pvir_Dia2013_COI	POR-A23	1	BD29297_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	CTTAGTGGCATTAATTCGDAATCC	141.62089
BD29297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34631	WGS84	BD29297	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29297_Rhar_Sim2018_COI	POR-A23	1	BD29297_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29297	Sample	BD29297	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A23	COI	45	BD29297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A23	CTGCTGCTATTGCTCATG	141.62089
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29299_Aamu_Bax2006_COI	POR-A21	1	BD29299_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	GCACAACCGGGATCTTTACTTC	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29299_Pcan_Bot2011_IGS	POR-A21	1	BD29299_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A21	IGS	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	CGTAATCCTCAGTACTGGCTA	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29299_Pper_Dia2013_COI	POR-A21	1	BD29299_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	CTTAGTGGCATTAATTCGDAATCC	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29299_Sspa_Oph2007_COI	POR-A21	1	BD29299_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	GAAGGCCTTCCGATGATC	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.246	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29299_Upin_Bot2011_COI	POR-A21	1	BD29299_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Present	Sample	Upin_Bot2011_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	CTTTAATTACAGCGTTTTTATTGTTGT	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29299_Vgib_Wil2025_COI	POR-A21	1	BD29299_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	CCCCTCTTTACAAGTTCTTTAGGGT	141.61823
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29300_Asen_Wil2025_COI	POR-A20	1	BD29300_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	GTTGTTGTTACAACACATGCTCTTTTG	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29300_Cjap_Sim2018_COI	POR-A20	1	BD29300_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29300_Esin_And2018_Cyb	POR-A20	1	BD29300_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A20	Cytb	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	ACCCCTCCTCATATCCAACCA	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29300_Mgig_Bot2012_COI	POR-A20	1	BD29300_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	TCTTATTCGTTGGAGACTTTATAACCCT	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29300_Mjap_Gib2022_COI	POR-A20	1	BD29300_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	AATTTAATTGTGACTAGGCATGGTCTG	141.61686
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29301_Cmae_Bot2011_COI	POR-A19	1	BD29301_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	ATGAACAGTCTATCCTCCTTTAG	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29301_Hsan_Wil2021_COI	POR-A19	1	BD29301_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	CCTGGGCCGGTATAGTAGGT	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	36.01	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29301_Mros_Gib2022_COI	POR-A19	1	BD29301_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Present	Sample	Mros_Gib2022_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29301_Msal_Bot2012_COI	POR-A19	1	BD29301_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	GYTAGTTCCRATGATGTTAGCTG	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29301_Mstr_Wil2021_COI	POR-A19	1	BD29301_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	GTAGTGGTAACTACTCATGCTTTA	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29301_Pvir_Dia2013_COI	POR-A19	1	BD29301_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	CTTAGTGGCATTAATTCGDAATCC	141.6153
BD29301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD29301	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29301_Rhar_Sim2018_COI	POR-A19	1	BD29301_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29301	Sample	BD29301	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A19	COI	45	BD29301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A19	CTGCTGCTATTGCTCATG	141.6153
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29303_Aamu_Bax2006_COI	POR-A17	1	BD29303_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	GCACAACCGGGATCTTTACTTC	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29303_Pcan_Bot2011_IGS	POR-A17	1	BD29303_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A17	IGS	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	CGTAATCCTCAGTACTGGCTA	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29303_Pper_Dia2013_COI	POR-A17	1	BD29303_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	CTTAGTGGCATTAATTCGDAATCC	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.721	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29303_Sspa_Oph2007_COI	POR-A17	1	BD29303_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	GAAGGCCTTCCGATGATC	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.118	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29303_Upin_Bot2011_COI	POR-A17	1	BD29303_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Present	Sample	Upin_Bot2011_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	CTTTAATTACAGCGTTTTTATTGTTGT	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29303_Vgib_Wil2025_COI	POR-A17	1	BD29303_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	CCCCTCTTTACAAGTTCTTTAGGGT	141.61256
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29304_Asen_Wil2025_COI	POR-A16	1	BD29304_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	GTTGTTGTTACAACACATGCTCTTTTG	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29304_Cjap_Sim2018_COI	POR-A16	1	BD29304_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29304_Esin_And2018_Cyb	POR-A16	1	BD29304_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A16	Cytb	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	ACCCCTCCTCATATCCAACCA	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29304_Mgig_Bot2012_COI	POR-A16	1	BD29304_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	TCTTATTCGTTGGAGACTTTATAACCCT	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29304_Mjap_Gib2022_COI	POR-A16	1	BD29304_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	AATTTAATTGTGACTAGGCATGGTCTG	141.62101
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29305_Cmae_Bot2011_COI	POR-A15	1	BD29305_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	ATGAACAGTCTATCCTCCTTTAG	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29305_Hsan_Wil2021_COI	POR-A15	1	BD29305_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	CCTGGGCCGGTATAGTAGGT	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29305_Mros_Gib2022_COI	POR-A15	1	BD29305_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29305_Msal_Bot2012_COI	POR-A15	1	BD29305_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	GYTAGTTCCRATGATGTTAGCTG	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29305_Mstr_Wil2021_COI	POR-A15	1	BD29305_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	GTAGTGGTAACTACTCATGCTTTA	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29305_Pvir_Dia2013_COI	POR-A15	1	BD29305_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	CTTAGTGGCATTAATTCGDAATCC	141.6203
BD29305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34732	WGS84	BD29305	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29305_Rhar_Sim2018_COI	POR-A15	1	BD29305_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29305	Sample	BD29305	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A15	COI	45	BD29305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A15	CTGCTGCTATTGCTCATG	141.6203
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29307_Aamu_Bax2006_COI	POR-A13	1	BD29307_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	GCACAACCGGGATCTTTACTTC	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29307_Pcan_Bot2011_IGS	POR-A13	1	BD29307_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A13	IGS	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	CGTAATCCTCAGTACTGGCTA	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29307_Pper_Dia2013_COI	POR-A13	1	BD29307_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	CTTAGTGGCATTAATTCGDAATCC	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29307_Sspa_Oph2007_COI	POR-A13	1	BD29307_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	GAAGGCCTTCCGATGATC	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.709	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29307_Upin_Bot2011_COI	POR-A13	1	BD29307_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Present	Sample	Upin_Bot2011_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	CTTTAATTACAGCGTTTTTATTGTTGT	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29307_Vgib_Wil2025_COI	POR-A13	1	BD29307_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	CCCCTCTTTACAAGTTCTTTAGGGT	141.61754
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29308_Asen_Wil2025_COI	POR-A12	1	BD29308_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	GTTGTTGTTACAACACATGCTCTTTTG	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29308_Cjap_Sim2018_COI	POR-A12	1	BD29308_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29308_Esin_And2018_Cyb	POR-A12	1	BD29308_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A12	Cytb	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	ACCCCTCCTCATATCCAACCA	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29308_Mgig_Bot2012_COI	POR-A12	1	BD29308_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	TCTTATTCGTTGGAGACTTTATAACCCT	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29308_Mjap_Gib2022_COI	POR-A12	1	BD29308_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	AATTTAATTGTGACTAGGCATGGTCTG	141.61615
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29309_Cmae_Bot2011_COI	POR-A11	1	BD29309_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	ATGAACAGTCTATCCTCCTTTAG	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29309_Hsan_Wil2021_COI	POR-A11	1	BD29309_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	CCTGGGCCGGTATAGTAGGT	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29309_Mros_Gib2022_COI	POR-A11	1	BD29309_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29309_Msal_Bot2012_COI	POR-A11	1	BD29309_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	GYTAGTTCCRATGATGTTAGCTG	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29309_Mstr_Wil2021_COI	POR-A11	1	BD29309_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	GTAGTGGTAACTACTCATGCTTTA	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29309_Pvir_Dia2013_COI	POR-A11	1	BD29309_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	CTTAGTGGCATTAATTCGDAATCC	141.61467
BD29309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29309	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29309_Rhar_Sim2018_COI	POR-A11	1	BD29309_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29309	Sample	BD29309	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A11	COI	45	BD29309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A11	CTGCTGCTATTGCTCATG	141.61467
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29310_Aamu_Bax2006_COI	POR-A10	1	BD29310_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	GCACAACCGGGATCTTTACTTC	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29310_Pcan_Bot2011_IGS	POR-A10	1	BD29310_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A10	IGS	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	CGTAATCCTCAGTACTGGCTA	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29310_Pper_Dia2013_COI	POR-A10	1	BD29310_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	CTTAGTGGCATTAATTCGDAATCC	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.18	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29310_Sspa_Oph2007_COI	POR-A10	1	BD29310_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	GAAGGCCTTCCGATGATC	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.058	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29310_Upin_Bot2011_COI	POR-A10	1	BD29310_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Present	Sample	Upin_Bot2011_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	CTTTAATTACAGCGTTTTTATTGTTGT	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29310_Vgib_Wil2025_COI	POR-A10	1	BD29310_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	CCCCTCTTTACAAGTTCTTTAGGGT	141.61328
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29311_Asen_Wil2025_COI	POR-A09	1	BD29311_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	GTTGTTGTTACAACACATGCTCTTTTG	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29311_Cjap_Sim2018_COI	POR-A09	1	BD29311_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29311_Esin_And2018_Cyb	POR-A09	1	BD29311_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A09	Cytb	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	ACCCCTCCTCATATCCAACCA	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29311_Mgig_Bot2012_COI	POR-A09	1	BD29311_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	TCTTATTCGTTGGAGACTTTATAACCCT	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29311_Mjap_Gib2022_COI	POR-A09	1	BD29311_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	AATTTAATTGTGACTAGGCATGGTCTG	141.61189
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29312_Cmae_Bot2011_COI	POR-A08	1	BD29312_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	ATGAACAGTCTATCCTCCTTTAG	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29312_Hsan_Wil2021_COI	POR-A08	1	BD29312_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	CCTGGGCCGGTATAGTAGGT	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29312_Mros_Gib2022_COI	POR-A08	1	BD29312_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29312_Msal_Bot2012_COI	POR-A08	1	BD29312_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	GYTAGTTCCRATGATGTTAGCTG	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29312_Mstr_Wil2021_COI	POR-A08	1	BD29312_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	GTAGTGGTAACTACTCATGCTTTA	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29312_Pvir_Dia2013_COI	POR-A08	1	BD29312_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	CTTAGTGGCATTAATTCGDAATCC	141.61066
BD29312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD29312	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29312_Rhar_Sim2018_COI	POR-A08	1	BD29312_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29312	Sample	BD29312	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A08	COI	45	BD29312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A08	CTGCTGCTATTGCTCATG	141.61066
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29314_Aamu_Bax2006_COI	POR-A06	1	BD29314_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	GCACAACCGGGATCTTTACTTC	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29314_Pcan_Bot2011_IGS	POR-A06	1	BD29314_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A06	IGS	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	CGTAATCCTCAGTACTGGCTA	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29314_Pper_Dia2013_COI	POR-A06	1	BD29314_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	CTTAGTGGCATTAATTCGDAATCC	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29314_Sspa_Oph2007_COI	POR-A06	1	BD29314_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	GAAGGCCTTCCGATGATC	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.536	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29314_Upin_Bot2011_COI	POR-A06	1	BD29314_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Present	Sample	Upin_Bot2011_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	CTTTAATTACAGCGTTTTTATTGTTGT	141.61969
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29315_Cjap_Sim2018_COI	POR-A05	1	BD29315_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29315_Esin_And2018_Cyb	POR-A05	1	BD29315_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A05	Cytb	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	ACCCCTCCTCATATCCAACCA	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29315_Mgig_Bot2012_COI	POR-A05	1	BD29315_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	TCTTATTCGTTGGAGACTTTATAACCCT	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29315_Mjap_Gib2022_COI	POR-A05	1	BD29315_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	AATTTAATTGTGACTAGGCATGGTCTG	141.61829
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29316_Cmae_Bot2011_COI	POR-A04	1	BD29316_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	ATGAACAGTCTATCCTCCTTTAG	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29316_Hsan_Wil2021_COI	POR-A04	1	BD29316_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	CCTGGGCCGGTATAGTAGGT	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29316_Mros_Gib2022_COI	POR-A04	1	BD29316_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29316_Msal_Bot2012_COI	POR-A04	1	BD29316_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	GYTAGTTCCRATGATGTTAGCTG	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29316_Mstr_Wil2021_COI	POR-A04	1	BD29316_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	GTAGTGGTAACTACTCATGCTTTA	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29316_Pvir_Dia2013_COI	POR-A04	1	BD29316_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	CTTAGTGGCATTAATTCGDAATCC	141.61684
BD29316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34841	WGS84	BD29316	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29316_Rhar_Sim2018_COI	POR-A04	1	BD29316_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29316	Sample	BD29316	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A04	COI	45	BD29316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A04	CTGCTGCTATTGCTCATG	141.61684
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29318_Aamu_Bax2006_COI	POR-A02	1	BD29318_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	GCACAACCGGGATCTTTACTTC	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29318_Pcan_Bot2011_IGS	POR-A02	1	BD29318_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A02	IGS	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	CGTAATCCTCAGTACTGGCTA	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29318_Pper_Dia2013_COI	POR-A02	1	BD29318_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	CTTAGTGGCATTAATTCGDAATCC	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29318_Sspa_Oph2007_COI	POR-A02	1	BD29318_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	GAAGGCCTTCCGATGATC	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.158	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29318_Upin_Bot2011_COI	POR-A02	1	BD29318_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Present	Sample	Upin_Bot2011_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	CTTTAATTACAGCGTTTTTATTGTTGT	141.62044
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29319_Cjap_Sim2018_COI	POR-A01	1	BD29319_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29319_Esin_And2018_Cyb	POR-A01	1	BD29319_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A01	Cytb	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	ACCCCTCCTCATATCCAACCA	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29319_Mgig_Bot2012_COI	POR-A01	1	BD29319_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	TCTTATTCGTTGGAGACTTTATAACCCT	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29319_Mjap_Gib2022_COI	POR-A01	1	BD29319_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	AATTTAATTGTGACTAGGCATGGTCTG	141.61035
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29321_Aamu_Bax2006_COI	KPT-A34	1	BD29321_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	GCACAACCGGGATCTTTACTTC	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29321_Pcan_Bot2011_IGS	KPT-A34	1	BD29321_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A34	IGS	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	CGTAATCCTCAGTACTGGCTA	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29321_Pper_Dia2013_COI	KPT-A34	1	BD29321_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	CTTAGTGGCATTAATTCGDAATCC	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29321_Sspa_Oph2007_COI	KPT-A34	1	BD29321_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	GAAGGCCTTCCGATGATC	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29321_Upin_Bot2011_COI	KPT-A34	1	BD29321_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	CTTTAATTACAGCGTTTTTATTGTTGT	137.7771
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29322_Cjap_Sim2018_COI	KPT-A33	1	BD29322_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29322_Esin_And2018_Cyb	KPT-A33	1	BD29322_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A33	Cytb	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	ACCCCTCCTCATATCCAACCA	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29322_Mgig_Bot2012_COI	KPT-A33	1	BD29322_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	TCTTATTCGTTGGAGACTTTATAACCCT	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29322_Mjap_Gib2022_COI	KPT-A33	1	BD29322_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	AATTTAATTGTGACTAGGCATGGTCTG	137.77745
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29323_Cmae_Bot2011_COI	KPT-A32	1	BD29323_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	ATGAACAGTCTATCCTCCTTTAG	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29323_Hsan_Wil2021_COI	KPT-A32	1	BD29323_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	CCTGGGCCGGTATAGTAGGT	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29323_Mros_Gib2022_COI	KPT-A32	1	BD29323_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29323_Msal_Bot2012_COI	KPT-A32	1	BD29323_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	GYTAGTTCCRATGATGTTAGCTG	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29323_Mstr_Wil2021_COI	KPT-A32	1	BD29323_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	GTAGTGGTAACTACTCATGCTTTA	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29323_Pvir_Dia2013_COI	KPT-A32	1	BD29323_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	CTTAGTGGCATTAATTCGDAATCC	137.77736
BD29323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95839	WGS84	BD29323	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29323_Rhar_Sim2018_COI	KPT-A32	1	BD29323_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29323	Sample	BD29323	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A32	COI	45	BD29323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A32	CTGCTGCTATTGCTCATG	137.77736
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29325_Aamu_Bax2006_COI	KPT-A30	1	BD29325_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	GCACAACCGGGATCTTTACTTC	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29325_Pcan_Bot2011_IGS	KPT-A30	1	BD29325_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A30	IGS	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	CGTAATCCTCAGTACTGGCTA	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29325_Pper_Dia2013_COI	KPT-A30	1	BD29325_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	CTTAGTGGCATTAATTCGDAATCC	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29325_Sspa_Oph2007_COI	KPT-A30	1	BD29325_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	GAAGGCCTTCCGATGATC	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29325_Upin_Bot2011_COI	KPT-A30	1	BD29325_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	CTTTAATTACAGCGTTTTTATTGTTGT	137.77845
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29326_Cjap_Sim2018_COI	KPT-A29	1	BD29326_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29326_Esin_And2018_Cyb	KPT-A29	1	BD29326_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A29	Cytb	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	ACCCCTCCTCATATCCAACCA	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29326_Mgig_Bot2012_COI	KPT-A29	1	BD29326_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	TCTTATTCGTTGGAGACTTTATAACCCT	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29326_Mjap_Gib2022_COI	KPT-A29	1	BD29326_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	AATTTAATTGTGACTAGGCATGGTCTG	137.77712
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29327_Cmae_Bot2011_COI	KPT-A28	1	BD29327_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	ATGAACAGTCTATCCTCCTTTAG	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29327_Hsan_Wil2021_COI	KPT-A28	1	BD29327_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	CCTGGGCCGGTATAGTAGGT	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29327_Mros_Gib2022_COI	KPT-A28	1	BD29327_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29327_Msal_Bot2012_COI	KPT-A28	1	BD29327_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	GYTAGTTCCRATGATGTTAGCTG	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29327_Mstr_Wil2021_COI	KPT-A28	1	BD29327_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	GTAGTGGTAACTACTCATGCTTTA	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29327_Pvir_Dia2013_COI	KPT-A28	1	BD29327_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	CTTAGTGGCATTAATTCGDAATCC	137.77558
BD29327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96017	WGS84	BD29327	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29327_Rhar_Sim2018_COI	KPT-A28	1	BD29327_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29327	Sample	BD29327	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A28	COI	45	BD29327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A28	CTGCTGCTATTGCTCATG	137.77558
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29329_Aamu_Bax2006_COI	KPT-A26	1	BD29329_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	GCACAACCGGGATCTTTACTTC	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29329_Pcan_Bot2011_IGS	KPT-A26	1	BD29329_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A26	IGS	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	CGTAATCCTCAGTACTGGCTA	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29329_Pper_Dia2013_COI	KPT-A26	1	BD29329_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	CTTAGTGGCATTAATTCGDAATCC	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29329_Sspa_Oph2007_COI	KPT-A26	1	BD29329_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	GAAGGCCTTCCGATGATC	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29329_Upin_Bot2011_COI	KPT-A26	1	BD29329_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	CTTTAATTACAGCGTTTTTATTGTTGT	137.77811
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29330_Cmae_Bot2011_COI	KPT-A25	1	BD29330_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	ATGAACAGTCTATCCTCCTTTAG	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29330_Hsan_Wil2021_COI	KPT-A25	1	BD29330_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	CCTGGGCCGGTATAGTAGGT	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29330_Mros_Gib2022_COI	KPT-A25	1	BD29330_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29330_Msal_Bot2012_COI	KPT-A25	1	BD29330_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	GYTAGTTCCRATGATGTTAGCTG	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29330_Mstr_Wil2021_COI	KPT-A25	1	BD29330_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	GTAGTGGTAACTACTCATGCTTTA	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29330_Pvir_Dia2013_COI	KPT-A25	1	BD29330_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	CTTAGTGGCATTAATTCGDAATCC	137.77937
BD29330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96016	WGS84	BD29330	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29330_Rhar_Sim2018_COI	KPT-A25	1	BD29330_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29330	Sample	BD29330	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A25	COI	45	BD29330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A25	CTGCTGCTATTGCTCATG	137.77937
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29332_Aamu_Bax2006_COI	KPT-A23	1	BD29332_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	GCACAACCGGGATCTTTACTTC	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29332_Pcan_Bot2011_IGS	KPT-A23	1	BD29332_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A23	IGS	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	CGTAATCCTCAGTACTGGCTA	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29332_Pper_Dia2013_COI	KPT-A23	1	BD29332_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	CTTAGTGGCATTAATTCGDAATCC	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29332_Sspa_Oph2007_COI	KPT-A23	1	BD29332_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	GAAGGCCTTCCGATGATC	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29332_Upin_Bot2011_COI	KPT-A23	1	BD29332_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	CTTTAATTACAGCGTTTTTATTGTTGT	137.77976
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29333_Cjap_Sim2018_COI	KPT-A22	1	BD29333_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29333_Esin_And2018_Cyb	KPT-A22	1	BD29333_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A22	Cytb	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	ACCCCTCCTCATATCCAACCA	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29333_Mgig_Bot2012_COI	KPT-A22	1	BD29333_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	TCTTATTCGTTGGAGACTTTATAACCCT	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29333_Mjap_Gib2022_COI	KPT-A22	1	BD29333_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	AATTTAATTGTGACTAGGCATGGTCTG	137.77849
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29334_Cmae_Bot2011_COI	KPT-A21	1	BD29334_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	ATGAACAGTCTATCCTCCTTTAG	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29334_Hsan_Wil2021_COI	KPT-A21	1	BD29334_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	CCTGGGCCGGTATAGTAGGT	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29334_Mros_Gib2022_COI	KPT-A21	1	BD29334_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29334_Msal_Bot2012_COI	KPT-A21	1	BD29334_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	GYTAGTTCCRATGATGTTAGCTG	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29334_Mstr_Wil2021_COI	KPT-A21	1	BD29334_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	GTAGTGGTAACTACTCATGCTTTA	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29334_Pvir_Dia2013_COI	KPT-A21	1	BD29334_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	CTTAGTGGCATTAATTCGDAATCC	137.77639
BD29334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96094	WGS84	BD29334	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29334_Rhar_Sim2018_COI	KPT-A21	1	BD29334_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29334	Sample	BD29334	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A21	COI	45	BD29334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A21	CTGCTGCTATTGCTCATG	137.77639
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29336_Aamu_Bax2006_COI	KPT-A19	1	BD29336_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	GCACAACCGGGATCTTTACTTC	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29336_Pcan_Bot2011_IGS	KPT-A19	1	BD29336_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A19	IGS	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	CGTAATCCTCAGTACTGGCTA	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29336_Pper_Dia2013_COI	KPT-A19	1	BD29336_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	CTTAGTGGCATTAATTCGDAATCC	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29336_Sspa_Oph2007_COI	KPT-A19	1	BD29336_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	GAAGGCCTTCCGATGATC	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29336_Upin_Bot2011_COI	KPT-A19	1	BD29336_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	CTTTAATTACAGCGTTTTTATTGTTGT	137.77707
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29337_Cjap_Sim2018_COI	KPT-A18	1	BD29337_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29337_Esin_And2018_Cyb	KPT-A18	1	BD29337_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A18	Cytb	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	ACCCCTCCTCATATCCAACCA	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29337_Mgig_Bot2012_COI	KPT-A18	1	BD29337_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	TCTTATTCGTTGGAGACTTTATAACCCT	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29337_Mjap_Gib2022_COI	KPT-A18	1	BD29337_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	AATTTAATTGTGACTAGGCATGGTCTG	137.7782
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29338_Cmae_Bot2011_COI	KPT-A17	1	BD29338_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	ATGAACAGTCTATCCTCCTTTAG	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29338_Hsan_Wil2021_COI	KPT-A17	1	BD29338_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	CCTGGGCCGGTATAGTAGGT	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29338_Mros_Gib2022_COI	KPT-A17	1	BD29338_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29338_Msal_Bot2012_COI	KPT-A17	1	BD29338_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	GYTAGTTCCRATGATGTTAGCTG	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29338_Mstr_Wil2021_COI	KPT-A17	1	BD29338_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	GTAGTGGTAACTACTCATGCTTTA	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29338_Pvir_Dia2013_COI	KPT-A17	1	BD29338_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	CTTAGTGGCATTAATTCGDAATCC	137.77943
BD29338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9619	WGS84	BD29338	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29338_Rhar_Sim2018_COI	KPT-A17	1	BD29338_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29338	Sample	BD29338	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A17	COI	45	BD29338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A17	CTGCTGCTATTGCTCATG	137.77943
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29340_Cjap_Sim2018_COI	KPT-A15	1	BD29340_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29340_Esin_And2018_Cyb	KPT-A15	1	BD29340_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A15	Cytb	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	ACCCCTCCTCATATCCAACCA	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29340_Mgig_Bot2012_COI	KPT-A15	1	BD29340_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	TCTTATTCGTTGGAGACTTTATAACCCT	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29340_Mjap_Gib2022_COI	KPT-A15	1	BD29340_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	AATTTAATTGTGACTAGGCATGGTCTG	137.77982
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29341_Cmae_Bot2011_COI	KPT-A14	1	BD29341_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	ATGAACAGTCTATCCTCCTTTAG	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29341_Hsan_Wil2021_COI	KPT-A14	1	BD29341_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	CCTGGGCCGGTATAGTAGGT	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29341_Mros_Gib2022_COI	KPT-A14	1	BD29341_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29341_Msal_Bot2012_COI	KPT-A14	1	BD29341_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	GYTAGTTCCRATGATGTTAGCTG	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29341_Mstr_Wil2021_COI	KPT-A14	1	BD29341_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	GTAGTGGTAACTACTCATGCTTTA	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29341_Pvir_Dia2013_COI	KPT-A14	1	BD29341_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	CTTAGTGGCATTAATTCGDAATCC	137.7785
BD29341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29341	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29341_Rhar_Sim2018_COI	KPT-A14	1	BD29341_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29341	Sample	BD29341	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A14	COI	45	BD29341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A14	CTGCTGCTATTGCTCATG	137.7785
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29343_Aamu_Bax2006_COI	KPT-A12	1	BD29343_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	GCACAACCGGGATCTTTACTTC	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29343_Pcan_Bot2011_IGS	KPT-A12	1	BD29343_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A12	IGS	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	CGTAATCCTCAGTACTGGCTA	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29343_Pper_Dia2013_COI	KPT-A12	1	BD29343_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	CTTAGTGGCATTAATTCGDAATCC	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29343_Sspa_Oph2007_COI	KPT-A12	1	BD29343_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	GAAGGCCTTCCGATGATC	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29343_Upin_Bot2011_COI	KPT-A12	1	BD29343_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	CTTTAATTACAGCGTTTTTATTGTTGT	137.77589
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29344_Cjap_Sim2018_COI	KPT-A11	1	BD29344_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29344_Esin_And2018_Cyb	KPT-A11	1	BD29344_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A11	Cytb	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	ACCCCTCCTCATATCCAACCA	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29344_Mgig_Bot2012_COI	KPT-A11	1	BD29344_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	TCTTATTCGTTGGAGACTTTATAACCCT	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29344_Mjap_Gib2022_COI	KPT-A11	1	BD29344_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	AATTTAATTGTGACTAGGCATGGTCTG	137.77455
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29345_Cmae_Bot2011_COI	KPT-A10	1	BD29345_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	ATGAACAGTCTATCCTCCTTTAG	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29345_Hsan_Wil2021_COI	KPT-A10	1	BD29345_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	CCTGGGCCGGTATAGTAGGT	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29345_Mros_Gib2022_COI	KPT-A10	1	BD29345_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29345_Msal_Bot2012_COI	KPT-A10	1	BD29345_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	GYTAGTTCCRATGATGTTAGCTG	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29345_Mstr_Wil2021_COI	KPT-A10	1	BD29345_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	GTAGTGGTAACTACTCATGCTTTA	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29345_Pvir_Dia2013_COI	KPT-A10	1	BD29345_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	CTTAGTGGCATTAATTCGDAATCC	137.77399
BD29345	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96387	WGS84	BD29345	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29345_Rhar_Sim2018_COI	KPT-A10	1	BD29345_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29345	Sample	BD29345	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A10	COI	45	BD29345	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A10	CTGCTGCTATTGCTCATG	137.77399
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29347_Aamu_Bax2006_COI	KPT-A08	1	BD29347_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	GCACAACCGGGATCTTTACTTC	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29347_Pcan_Bot2011_IGS	KPT-A08	1	BD29347_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A08	IGS	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	CGTAATCCTCAGTACTGGCTA	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29347_Pper_Dia2013_COI	KPT-A08	1	BD29347_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	CTTAGTGGCATTAATTCGDAATCC	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29347_Sspa_Oph2007_COI	KPT-A08	1	BD29347_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	GAAGGCCTTCCGATGATC	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29347_Upin_Bot2011_COI	KPT-A08	1	BD29347_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	CTTTAATTACAGCGTTTTTATTGTTGT	137.77573
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29348_Cjap_Sim2018_COI	KPT-A07	1	BD29348_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29348_Esin_And2018_Cyb	KPT-A07	1	BD29348_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A07	Cytb	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	ACCCCTCCTCATATCCAACCA	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29348_Mgig_Bot2012_COI	KPT-A07	1	BD29348_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	TCTTATTCGTTGGAGACTTTATAACCCT	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29348_Mjap_Gib2022_COI	KPT-A07	1	BD29348_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	AATTTAATTGTGACTAGGCATGGTCTG	137.77697
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29349_Cmae_Bot2011_COI	KPT-A06	1	BD29349_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	ATGAACAGTCTATCCTCCTTTAG	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29349_Hsan_Wil2021_COI	KPT-A06	1	BD29349_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	CCTGGGCCGGTATAGTAGGT	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29349_Mros_Gib2022_COI	KPT-A06	1	BD29349_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29349_Msal_Bot2012_COI	KPT-A06	1	BD29349_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	GYTAGTTCCRATGATGTTAGCTG	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29349_Mstr_Wil2021_COI	KPT-A06	1	BD29349_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	GTAGTGGTAACTACTCATGCTTTA	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29349_Pvir_Dia2013_COI	KPT-A06	1	BD29349_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	CTTAGTGGCATTAATTCGDAATCC	137.77908
BD29349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96444	WGS84	BD29349	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29349_Rhar_Sim2018_COI	KPT-A06	1	BD29349_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29349	Sample	BD29349	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A06	COI	45	BD29349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A06	CTGCTGCTATTGCTCATG	137.77908
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29350_Aamu_Bax2006_COI	KPT-A05	1	BD29350_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	GCACAACCGGGATCTTTACTTC	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29350_Pcan_Bot2011_IGS	KPT-A05	1	BD29350_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A05	IGS	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	CGTAATCCTCAGTACTGGCTA	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29350_Pper_Dia2013_COI	KPT-A05	1	BD29350_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	CTTAGTGGCATTAATTCGDAATCC	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29350_Sspa_Oph2007_COI	KPT-A05	1	BD29350_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	GAAGGCCTTCCGATGATC	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29350_Upin_Bot2011_COI	KPT-A05	1	BD29350_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	CTTTAATTACAGCGTTTTTATTGTTGT	137.78049
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29351_Cjap_Sim2018_COI	KPT-A04	1	BD29351_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29351_Esin_And2018_Cyb	KPT-A04	1	BD29351_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A04	Cytb	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	ACCCCTCCTCATATCCAACCA	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29351_Mgig_Bot2012_COI	KPT-A04	1	BD29351_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	TCTTATTCGTTGGAGACTTTATAACCCT	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29351_Mjap_Gib2022_COI	KPT-A04	1	BD29351_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	AATTTAATTGTGACTAGGCATGGTCTG	137.77854
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29352_Cmae_Bot2011_COI	KPT-A03	1	BD29352_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	ATGAACAGTCTATCCTCCTTTAG	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29352_Hsan_Wil2021_COI	KPT-A03	1	BD29352_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	CCTGGGCCGGTATAGTAGGT	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29352_Mros_Gib2022_COI	KPT-A03	1	BD29352_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29352_Msal_Bot2012_COI	KPT-A03	1	BD29352_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	GYTAGTTCCRATGATGTTAGCTG	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29352_Mstr_Wil2021_COI	KPT-A03	1	BD29352_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	GTAGTGGTAACTACTCATGCTTTA	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29352_Pvir_Dia2013_COI	KPT-A03	1	BD29352_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	CTTAGTGGCATTAATTCGDAATCC	137.7773
BD29352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96533	WGS84	BD29352	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29352_Rhar_Sim2018_COI	KPT-A03	1	BD29352_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29352	Sample	BD29352	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A03	COI	45	BD29352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A03	CTGCTGCTATTGCTCATG	137.7773
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29354_Aamu_Bax2006_COI	PGI-A35	1	BD29354_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	GCACAACCGGGATCTTTACTTC	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29354_Pcan_Bot2011_IGS	PGI-A35	1	BD29354_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A35	IGS	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	CGTAATCCTCAGTACTGGCTA	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29354_Pper_Dia2013_COI	PGI-A35	1	BD29354_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	CTTAGTGGCATTAATTCGDAATCC	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29354_Sspa_Oph2007_COI	PGI-A35	1	BD29354_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	GAAGGCCTTCCGATGATC	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29354_Upin_Bot2011_COI	PGI-A35	1	BD29354_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	CTTTAATTACAGCGTTTTTATTGTTGT	137.76478
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29355_Cjap_Sim2018_COI	PGI-A34	1	BD29355_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29355_Esin_And2018_Cyb	PGI-A34	1	BD29355_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A34	Cytb	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	ACCCCTCCTCATATCCAACCA	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29355_Mgig_Bot2012_COI	PGI-A34	1	BD29355_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	TCTTATTCGTTGGAGACTTTATAACCCT	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29355_Mjap_Gib2022_COI	PGI-A34	1	BD29355_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	AATTTAATTGTGACTAGGCATGGTCTG	137.76598
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29356_Cmae_Bot2011_COI	PGI-A33	1	BD29356_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	ATGAACAGTCTATCCTCCTTTAG	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29356_Hsan_Wil2021_COI	PGI-A33	1	BD29356_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	CCTGGGCCGGTATAGTAGGT	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29356_Mros_Gib2022_COI	PGI-A33	1	BD29356_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29356_Msal_Bot2012_COI	PGI-A33	1	BD29356_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	GYTAGTTCCRATGATGTTAGCTG	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29356_Mstr_Wil2021_COI	PGI-A33	1	BD29356_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	GTAGTGGTAACTACTCATGCTTTA	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29356_Pvir_Dia2013_COI	PGI-A33	1	BD29356_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	CTTAGTGGCATTAATTCGDAATCC	137.76808
BD29356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02476	WGS84	BD29356	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29356_Rhar_Sim2018_COI	PGI-A33	1	BD29356_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29356	Sample	BD29356	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A33	COI	45	BD29356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A33	CTGCTGCTATTGCTCATG	137.76808
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29358_Aamu_Bax2006_COI	PGI-A31	1	BD29358_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	GCACAACCGGGATCTTTACTTC	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29358_Pcan_Bot2011_IGS	PGI-A31	1	BD29358_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A31	IGS	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	CGTAATCCTCAGTACTGGCTA	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29358_Pper_Dia2013_COI	PGI-A31	1	BD29358_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	CTTAGTGGCATTAATTCGDAATCC	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29358_Sspa_Oph2007_COI	PGI-A31	1	BD29358_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	GAAGGCCTTCCGATGATC	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29358_Upin_Bot2011_COI	PGI-A31	1	BD29358_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	CTTTAATTACAGCGTTTTTATTGTTGT	137.77129
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29359_Cjap_Sim2018_COI	PGI-A30	1	BD29359_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29359_Esin_And2018_Cyb	PGI-A30	1	BD29359_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A30	Cytb	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	ACCCCTCCTCATATCCAACCA	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29359_Mgig_Bot2012_COI	PGI-A30	1	BD29359_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	TCTTATTCGTTGGAGACTTTATAACCCT	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29359_Mjap_Gib2022_COI	PGI-A30	1	BD29359_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	AATTTAATTGTGACTAGGCATGGTCTG	137.77228
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29361_Aamu_Bax2006_COI	PGI-A28	1	BD29361_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	GCACAACCGGGATCTTTACTTC	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29361_Pcan_Bot2011_IGS	PGI-A28	1	BD29361_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A28	IGS	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	CGTAATCCTCAGTACTGGCTA	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29361_Pper_Dia2013_COI	PGI-A28	1	BD29361_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	CTTAGTGGCATTAATTCGDAATCC	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29361_Sspa_Oph2007_COI	PGI-A28	1	BD29361_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	GAAGGCCTTCCGATGATC	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29361_Upin_Bot2011_COI	PGI-A28	1	BD29361_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	CTTTAATTACAGCGTTTTTATTGTTGT	137.76915
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29362_Cjap_Sim2018_COI	PGI-A27	1	BD29362_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29362_Esin_And2018_Cyb	PGI-A27	1	BD29362_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A27	Cytb	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	ACCCCTCCTCATATCCAACCA	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29362_Mgig_Bot2012_COI	PGI-A27	1	BD29362_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	TCTTATTCGTTGGAGACTTTATAACCCT	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29362_Mjap_Gib2022_COI	PGI-A27	1	BD29362_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	AATTTAATTGTGACTAGGCATGGTCTG	137.76747
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29363_Cmae_Bot2011_COI	PGI-A26	1	BD29363_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	ATGAACAGTCTATCCTCCTTTAG	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29363_Hsan_Wil2021_COI	PGI-A26	1	BD29363_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	CCTGGGCCGGTATAGTAGGT	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29363_Mros_Gib2022_COI	PGI-A26	1	BD29363_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29363_Msal_Bot2012_COI	PGI-A26	1	BD29363_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	GYTAGTTCCRATGATGTTAGCTG	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29363_Mstr_Wil2021_COI	PGI-A26	1	BD29363_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	GTAGTGGTAACTACTCATGCTTTA	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29363_Pvir_Dia2013_COI	PGI-A26	1	BD29363_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	CTTAGTGGCATTAATTCGDAATCC	137.76572
BD29363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02392	WGS84	BD29363	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29363_Rhar_Sim2018_COI	PGI-A26	1	BD29363_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29363	Sample	BD29363	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A26	COI	45	BD29363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A26	CTGCTGCTATTGCTCATG	137.76572
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29365_Aamu_Bax2006_COI	PGI-A24	1	BD29365_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	GCACAACCGGGATCTTTACTTC	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29365_Pcan_Bot2011_IGS	PGI-A24	1	BD29365_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A24	IGS	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	CGTAATCCTCAGTACTGGCTA	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29365_Pper_Dia2013_COI	PGI-A24	1	BD29365_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	CTTAGTGGCATTAATTCGDAATCC	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29365_Sspa_Oph2007_COI	PGI-A24	1	BD29365_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	GAAGGCCTTCCGATGATC	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29365_Upin_Bot2011_COI	PGI-A24	1	BD29365_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	CTTTAATTACAGCGTTTTTATTGTTGT	137.76629
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29366_Cjap_Sim2018_COI	PGI-A23	1	BD29366_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29366_Esin_And2018_Cyb	PGI-A23	1	BD29366_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A23	Cytb	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	ACCCCTCCTCATATCCAACCA	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29366_Mgig_Bot2012_COI	PGI-A23	1	BD29366_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	TCTTATTCGTTGGAGACTTTATAACCCT	137.76796
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29367_Cmae_Bot2011_COI	PGI-A22	1	BD29367_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	ATGAACAGTCTATCCTCCTTTAG	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29367_Hsan_Wil2021_COI	PGI-A22	1	BD29367_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	CCTGGGCCGGTATAGTAGGT	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29367_Mros_Gib2022_COI	PGI-A22	1	BD29367_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29367_Msal_Bot2012_COI	PGI-A22	1	BD29367_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	GYTAGTTCCRATGATGTTAGCTG	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29367_Mstr_Wil2021_COI	PGI-A22	1	BD29367_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	GTAGTGGTAACTACTCATGCTTTA	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29367_Pvir_Dia2013_COI	PGI-A22	1	BD29367_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	CTTAGTGGCATTAATTCGDAATCC	137.76822
BD29367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02304	WGS84	BD29367	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29367_Rhar_Sim2018_COI	PGI-A22	1	BD29367_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29367	Sample	BD29367	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A22	COI	45	BD29367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A22	CTGCTGCTATTGCTCATG	137.76822
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29369_Aamu_Bax2006_COI	PGI-A20	1	BD29369_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	GCACAACCGGGATCTTTACTTC	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29369_Pcan_Bot2011_IGS	PGI-A20	1	BD29369_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A20	IGS	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	CGTAATCCTCAGTACTGGCTA	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29369_Pper_Dia2013_COI	PGI-A20	1	BD29369_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	CTTAGTGGCATTAATTCGDAATCC	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29369_Sspa_Oph2007_COI	PGI-A20	1	BD29369_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	GAAGGCCTTCCGATGATC	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29369_Upin_Bot2011_COI	PGI-A20	1	BD29369_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	CTTTAATTACAGCGTTTTTATTGTTGT	137.77337
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29370_Cmae_Bot2011_COI	PGI-A19	1	BD29370_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	ATGAACAGTCTATCCTCCTTTAG	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29370_Hsan_Wil2021_COI	PGI-A19	1	BD29370_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	CCTGGGCCGGTATAGTAGGT	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29370_Mros_Gib2022_COI	PGI-A19	1	BD29370_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29370_Msal_Bot2012_COI	PGI-A19	1	BD29370_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	GYTAGTTCCRATGATGTTAGCTG	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29370_Mstr_Wil2021_COI	PGI-A19	1	BD29370_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	GTAGTGGTAACTACTCATGCTTTA	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29370_Pvir_Dia2013_COI	PGI-A19	1	BD29370_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	CTTAGTGGCATTAATTCGDAATCC	137.77052
BD29370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02126	WGS84	BD29370	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29370_Rhar_Sim2018_COI	PGI-A19	1	BD29370_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29370	Sample	BD29370	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A19	COI	45	BD29370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A19	CTGCTGCTATTGCTCATG	137.77052
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29372_Aamu_Bax2006_COI	PGI-A17	1	BD29372_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	GCACAACCGGGATCTTTACTTC	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29372_Pcan_Bot2011_IGS	PGI-A17	1	BD29372_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A17	IGS	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	CGTAATCCTCAGTACTGGCTA	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29372_Pper_Dia2013_COI	PGI-A17	1	BD29372_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	CTTAGTGGCATTAATTCGDAATCC	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29372_Sspa_Oph2007_COI	PGI-A17	1	BD29372_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	GAAGGCCTTCCGATGATC	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29372_Upin_Bot2011_COI	PGI-A17	1	BD29372_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	CTTTAATTACAGCGTTTTTATTGTTGT	137.77262
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29373_Cjap_Sim2018_COI	PGI-A16	1	BD29373_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29373_Esin_And2018_Cyb	PGI-A16	1	BD29373_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A16	Cytb	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	ACCCCTCCTCATATCCAACCA	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29373_Mgig_Bot2012_COI	PGI-A16	1	BD29373_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	TCTTATTCGTTGGAGACTTTATAACCCT	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29373_Mjap_Gib2022_COI	PGI-A16	1	BD29373_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	AATTTAATTGTGACTAGGCATGGTCTG	137.77345
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29374_Cmae_Bot2011_COI	PGI-A15	1	BD29374_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	ATGAACAGTCTATCCTCCTTTAG	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29374_Hsan_Wil2021_COI	PGI-A15	1	BD29374_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	CCTGGGCCGGTATAGTAGGT	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29374_Mros_Gib2022_COI	PGI-A15	1	BD29374_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29374_Msal_Bot2012_COI	PGI-A15	1	BD29374_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	GYTAGTTCCRATGATGTTAGCTG	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29374_Mstr_Wil2021_COI	PGI-A15	1	BD29374_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	GTAGTGGTAACTACTCATGCTTTA	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29374_Pvir_Dia2013_COI	PGI-A15	1	BD29374_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	CTTAGTGGCATTAATTCGDAATCC	137.7716
BD29374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02039	WGS84	BD29374	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29374_Rhar_Sim2018_COI	PGI-A15	1	BD29374_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29374	Sample	BD29374	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A15	COI	45	BD29374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A15	CTGCTGCTATTGCTCATG	137.7716
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29376_Aamu_Bax2006_COI	PGI-A13	1	BD29376_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	GCACAACCGGGATCTTTACTTC	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29376_Pcan_Bot2011_IGS	PGI-A13	1	BD29376_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A13	IGS	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	CGTAATCCTCAGTACTGGCTA	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29376_Pper_Dia2013_COI	PGI-A13	1	BD29376_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	CTTAGTGGCATTAATTCGDAATCC	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29376_Sspa_Oph2007_COI	PGI-A13	1	BD29376_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	GAAGGCCTTCCGATGATC	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29376_Upin_Bot2011_COI	PGI-A13	1	BD29376_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	CTTTAATTACAGCGTTTTTATTGTTGT	137.76788
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29377_Cjap_Sim2018_COI	PGI-A12	1	BD29377_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29377_Esin_And2018_Cyb	PGI-A12	1	BD29377_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A12	Cytb	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	ACCCCTCCTCATATCCAACCA	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29377_Mgig_Bot2012_COI	PGI-A12	1	BD29377_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	TCTTATTCGTTGGAGACTTTATAACCCT	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29377_Mjap_Gib2022_COI	PGI-A12	1	BD29377_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	AATTTAATTGTGACTAGGCATGGTCTG	137.7662
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29378_Cmae_Bot2011_COI	PGI-A11	1	BD29378_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	ATGAACAGTCTATCCTCCTTTAG	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29378_Hsan_Wil2021_COI	PGI-A11	1	BD29378_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	CCTGGGCCGGTATAGTAGGT	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29378_Mros_Gib2022_COI	PGI-A11	1	BD29378_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29378_Msal_Bot2012_COI	PGI-A11	1	BD29378_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	GYTAGTTCCRATGATGTTAGCTG	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29378_Mstr_Wil2021_COI	PGI-A11	1	BD29378_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	GTAGTGGTAACTACTCATGCTTTA	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29378_Pvir_Dia2013_COI	PGI-A11	1	BD29378_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	CTTAGTGGCATTAATTCGDAATCC	137.76389
BD29378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01995	WGS84	BD29378	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29378_Rhar_Sim2018_COI	PGI-A11	1	BD29378_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29378	Sample	BD29378	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A11	COI	45	BD29378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A11	CTGCTGCTATTGCTCATG	137.76389
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29380_Cjap_Sim2018_COI	PGI-A09	1	BD29380_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29380_Esin_And2018_Cyb	PGI-A09	1	BD29380_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A09	Cytb	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	ACCCCTCCTCATATCCAACCA	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29380_Mgig_Bot2012_COI	PGI-A09	1	BD29380_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	TCTTATTCGTTGGAGACTTTATAACCCT	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29380_Mjap_Gib2022_COI	PGI-A09	1	BD29380_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	AATTTAATTGTGACTAGGCATGGTCTG	137.76659
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29381_Cmae_Bot2011_COI	PGI-A08	1	BD29381_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	ATGAACAGTCTATCCTCCTTTAG	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29381_Hsan_Wil2021_COI	PGI-A08	1	BD29381_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	CCTGGGCCGGTATAGTAGGT	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29381_Mros_Gib2022_COI	PGI-A08	1	BD29381_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29381_Msal_Bot2012_COI	PGI-A08	1	BD29381_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	GYTAGTTCCRATGATGTTAGCTG	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29381_Mstr_Wil2021_COI	PGI-A08	1	BD29381_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	GTAGTGGTAACTACTCATGCTTTA	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29381_Pvir_Dia2013_COI	PGI-A08	1	BD29381_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	CTTAGTGGCATTAATTCGDAATCC	137.76859
BD29381	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01879	WGS84	BD29381	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29381_Rhar_Sim2018_COI	PGI-A08	1	BD29381_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29381	Sample	BD29381	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A08	COI	45	BD29381	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A08	CTGCTGCTATTGCTCATG	137.76859
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29383_Aamu_Bax2006_COI	PGI-A06	1	BD29383_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	GCACAACCGGGATCTTTACTTC	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29383_Pcan_Bot2011_IGS	PGI-A06	1	BD29383_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A06	IGS	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	CGTAATCCTCAGTACTGGCTA	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29383_Pper_Dia2013_COI	PGI-A06	1	BD29383_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	CTTAGTGGCATTAATTCGDAATCC	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29383_Sspa_Oph2007_COI	PGI-A06	1	BD29383_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	GAAGGCCTTCCGATGATC	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29383_Upin_Bot2011_COI	PGI-A06	1	BD29383_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	CTTTAATTACAGCGTTTTTATTGTTGT	137.77222
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29384_Cjap_Sim2018_COI	PGI-A02	1	BD29384_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29384_Esin_And2018_Cyb	PGI-A02	1	BD29384_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A02	Cytb	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	ACCCCTCCTCATATCCAACCA	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29384_Mgig_Bot2012_COI	PGI-A02	1	BD29384_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	TCTTATTCGTTGGAGACTTTATAACCCT	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29384_Mjap_Gib2022_COI	PGI-A02	1	BD29384_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	AATTTAATTGTGACTAGGCATGGTCTG	137.76551
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29385_Cmae_Bot2011_COI	PGI-A03	1	BD29385_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	ATGAACAGTCTATCCTCCTTTAG	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29385_Hsan_Wil2021_COI	PGI-A03	1	BD29385_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	CCTGGGCCGGTATAGTAGGT	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29385_Mros_Gib2022_COI	PGI-A03	1	BD29385_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29385_Msal_Bot2012_COI	PGI-A03	1	BD29385_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	GYTAGTTCCRATGATGTTAGCTG	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29385_Mstr_Wil2021_COI	PGI-A03	1	BD29385_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	GTAGTGGTAACTACTCATGCTTTA	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29385_Pvir_Dia2013_COI	PGI-A03	1	BD29385_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	CTTAGTGGCATTAATTCGDAATCC	137.76775
BD29385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01777	WGS84	BD29385	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29385_Rhar_Sim2018_COI	PGI-A03	1	BD29385_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29385	Sample	BD29385	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A03	COI	45	BD29385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A03	CTGCTGCTATTGCTCATG	137.76775
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29387_Aamu_Bax2006_COI	PGI-A04	1	BD29387_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	GCACAACCGGGATCTTTACTTC	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29387_Pcan_Bot2011_IGS	PGI-A04	1	BD29387_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A04	IGS	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	CGTAATCCTCAGTACTGGCTA	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29387_Pper_Dia2013_COI	PGI-A04	1	BD29387_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	CTTAGTGGCATTAATTCGDAATCC	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29387_Sspa_Oph2007_COI	PGI-A04	1	BD29387_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	GAAGGCCTTCCGATGATC	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29387_Upin_Bot2011_COI	PGI-A04	1	BD29387_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	CTTTAATTACAGCGTTTTTATTGTTGT	137.7696
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29388_Cjap_Sim2018_COI	PGI-A01	1	BD29388_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29388_Esin_And2018_Cyb	PGI-A01	1	BD29388_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A01	Cytb	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	ACCCCTCCTCATATCCAACCA	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29388_Mgig_Bot2012_COI	PGI-A01	1	BD29388_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	TCTTATTCGTTGGAGACTTTATAACCCT	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29388_Mjap_Gib2022_COI	PGI-A01	1	BD29388_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	AATTTAATTGTGACTAGGCATGGTCTG	137.76432
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29389_Cmae_Bot2011_COI	KPT-A01	1	BD29389_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	ATGAACAGTCTATCCTCCTTTAG	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29389_Hsan_Wil2021_COI	KPT-A01	1	BD29389_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	CCTGGGCCGGTATAGTAGGT	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29389_Mros_Gib2022_COI	KPT-A01	1	BD29389_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29389_Msal_Bot2012_COI	KPT-A01	1	BD29389_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	GYTAGTTCCRATGATGTTAGCTG	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29389_Mstr_Wil2021_COI	KPT-A01	1	BD29389_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	GTAGTGGTAACTACTCATGCTTTA	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29389_Pvir_Dia2013_COI	KPT-A01	1	BD29389_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	CTTAGTGGCATTAATTCGDAATCC	137.7746
BD29389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96542	WGS84	BD29389	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29389_Rhar_Sim2018_COI	KPT-A01	1	BD29389_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29389	Sample	BD29389	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A01	COI	45	BD29389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A01	CTGCTGCTATTGCTCATG	137.7746
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42627_Hsan_Wil2021_COI	ADV-01	1	BD42627_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-01	COI	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	CCTGGGCCGGTATAGTAGGT	144.9092464
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42627_Mros_Gib2022_COI	ADV-01	1	BD42627_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-01	COI	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9092464
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42627_Pvir_Dia2013_COI	ADV-01	1	BD42627_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-01	COI	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	CTTAGTGGCATTAATTCGDAATCC	144.9092464
BD42627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82108727	WGS84	BD42627	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42627_Rhar_Sim2018_COI	ADV-01	1	BD42627_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42627	Sample	BD42627	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-01	COI	45	BD42627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-01	CTGCTGCTATTGCTCATG	144.9092464
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42629_Pper_Dia2013_COI	ADV-03	1	BD42629_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-03	COI	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	CTTAGTGGCATTAATTCGDAATCC	144.9094286
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82136752	WGS84	BD42629	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42629_Vgib_Wil2025_COI	ADV-03	0	BD42629_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-03	COI	45	BD42629	1.00E-05	Nil	Australia	ADV-03	CCCCTCTTTACAAGTTCTTTAGGGT	144.9094286
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42630_Hsan_Wil2021_COI	ADV-04	1	BD42630_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-04	COI	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	CCTGGGCCGGTATAGTAGGT	144.9090642
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42630_Mros_Gib2022_COI	ADV-04	1	BD42630_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-04	COI	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9090642
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42630_Pvir_Dia2013_COI	ADV-04	1	BD42630_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-04	COI	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	CTTAGTGGCATTAATTCGDAATCC	144.9090642
BD42630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82080701	WGS84	BD42630	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42630_Rhar_Sim2018_COI	ADV-04	1	BD42630_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42630	Sample	BD42630	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-04	COI	45	BD42630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-04	CTGCTGCTATTGCTCATG	144.9090642
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42632_Pper_Dia2013_COI	ADV-06	1	BD42632_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-06	COI	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	CTTAGTGGCATTAATTCGDAATCC	144.8998675
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8279042	WGS84	BD42632	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42632_Vgib_Wil2025_COI	ADV-06	0	BD42632_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-06	COI	45	BD42632	1.00E-05	Nil	Australia	ADV-06	CCCCTCTTTACAAGTTCTTTAGGGT	144.8998675
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82764214	WGS84	BD42633	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	33.15	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42633_Asen_Wil2025_COI	ADV-07	0	BD42633_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Present	Sample	Asen_Wil2025_COI	ADV-07	COI	45	BD42633	1.00E-05	Nil	Australia	ADV-07	GTTGTTGTTACAACACATGCTCTTTTG	144.8992714
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42633_Cjap_Sim2018_COI	ADV-07	1	BD42633_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-07	COI	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.8992714
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42633_Esin_And2018_Cyb	ADV-07	1	BD42633_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-07	Cytb	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	ACCCCTCCTCATATCCAACCA	144.8992714
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42633_Mjap_Gib2022_COI	ADV-07	1	BD42633_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-07	COI	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	AATTTAATTGTGACTAGGCATGGTCTG	144.8992714
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	28.141	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42634_Hsan_Wil2021_COI	ADV-08	1	BD42634_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-08	COI	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	CCTGGGCCGGTATAGTAGGT	144.8995694
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42634_Mros_Gib2022_COI	ADV-08	1	BD42634_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-08	COI	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.8995694
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42634_Pvir_Dia2013_COI	ADV-08	1	BD42634_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-08	COI	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	CTTAGTGGCATTAATTCGDAATCC	144.8995694
BD42634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82777317	WGS84	BD42634	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42634_Rhar_Sim2018_COI	ADV-08	1	BD42634_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42634	Sample	BD42634	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-08	COI	45	BD42634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-08	CTGCTGCTATTGCTCATG	144.8995694
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42636_Pper_Dia2013_COI	ADV-10	1	BD42636_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-10	COI	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	CTTAGTGGCATTAATTCGDAATCC	144.9215243
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81817395	WGS84	BD42636	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42636_Vgib_Wil2025_COI	ADV-10	0	BD42636_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-10	COI	45	BD42636	1.00E-05	Nil	Australia	ADV-10	CCCCTCTTTACAAGTTCTTTAGGGT	144.9215243
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82816625	WGS84	BD42637	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	37.261	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42637_Asen_Wil2025_COI	ADV-11	0	BD42637_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Present	Sample	Asen_Wil2025_COI	ADV-11	COI	45	BD42637	1.00E-05	Nil	Australia	ADV-11	GTTGTTGTTACAACACATGCTCTTTTG	144.9004635
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42637_Cjap_Sim2018_COI	ADV-11	1	BD42637_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-11	COI	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9004635
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42637_Esin_And2018_Cyb	ADV-11	1	BD42637_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-11	Cytb	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	ACCCCTCCTCATATCCAACCA	144.9004635
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42637_Mjap_Gib2022_COI	ADV-11	1	BD42637_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-11	COI	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	AATTTAATTGTGACTAGGCATGGTCTG	144.9004635
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	27.005	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42638_Hsan_Wil2021_COI	ADV-12	1	BD42638_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-12	COI	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	CCTGGGCCGGTATAGTAGGT	144.9287502
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42638_Mros_Gib2022_COI	ADV-12	1	BD42638_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-12	COI	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9287502
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42638_Pvir_Dia2013_COI	ADV-12	1	BD42638_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-12	COI	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	CTTAGTGGCATTAATTCGDAATCC	144.9287502
BD42638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84243228	WGS84	BD42638	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42638_Rhar_Sim2018_COI	ADV-12	1	BD42638_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42638	Sample	BD42638	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-12	COI	45	BD42638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-12	CTGCTGCTATTGCTCATG	144.9287502
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84254362	WGS84	BD42640	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.381	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42640_Asen_Wil2025_COI	ADV-14	0	BD42640_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Present	Sample	Asen_Wil2025_COI	ADV-14	COI	45	BD42640	1.00E-05	Nil	Australia	ADV-14	GTTGTTGTTACAACACATGCTCTTTTG	144.9293007
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42640_Cjap_Sim2018_COI	ADV-14	1	BD42640_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-14	COI	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9293007
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42640_Esin_And2018_Cyb	ADV-14	1	BD42640_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-14	Cytb	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	ACCCCTCCTCATATCCAACCA	144.9293007
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42640_Mjap_Gib2022_COI	ADV-14	1	BD42640_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-14	COI	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	AATTTAATTGTGACTAGGCATGGTCTG	144.9293007
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.7	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42641_Hsan_Wil2021_COI	ADV-15	1	BD42641_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-15	COI	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	CCTGGGCCGGTATAGTAGGT	144.9281998
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42641_Mros_Gib2022_COI	ADV-15	1	BD42641_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-15	COI	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9281998
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42641_Pvir_Dia2013_COI	ADV-15	1	BD42641_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-15	COI	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	CTTAGTGGCATTAATTCGDAATCC	144.9281998
BD42641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84232086	WGS84	BD42641	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42641_Rhar_Sim2018_COI	ADV-15	1	BD42641_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42641	Sample	BD42641	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-15	COI	45	BD42641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-15	CTGCTGCTATTGCTCATG	144.9281998
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42643_Pper_Dia2013_COI	ADV-17	1	BD42643_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-17	COI	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	CTTAGTGGCATTAATTCGDAATCC	144.9217039
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81869538	WGS84	BD42643	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42643_Vgib_Wil2025_COI	ADV-17	0	BD42643_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-17	COI	45	BD42643	1.00E-05	Nil	Australia	ADV-17	CCCCTCTTTACAAGTTCTTTAGGGT	144.9217039
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81843466	WGS84	BD42644	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42644_Asen_Wil2025_COI	ADV-18	0	BD42644_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-18	COI	45	BD42644	1.00E-05	Nil	Australia	ADV-18	GTTGTTGTTACAACACATGCTCTTTTG	144.9216141
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42644_Cjap_Sim2018_COI	ADV-18	1	BD42644_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-18	COI	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9216141
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42644_Esin_And2018_Cyb	ADV-18	1	BD42644_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-18	Cytb	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	ACCCCTCCTCATATCCAACCA	144.9216141
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42644_Mjap_Gib2022_COI	ADV-18	1	BD42644_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-18	COI	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	AATTTAATTGTGACTAGGCATGGTCTG	144.9216141
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42645_Hsan_Wil2021_COI	ADV-19	1	BD42645_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-19	COI	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	CCTGGGCCGGTATAGTAGGT	144.9217938
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42645_Mros_Gib2022_COI	ADV-19	1	BD42645_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-19	COI	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9217938
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42645_Pvir_Dia2013_COI	ADV-19	1	BD42645_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-19	COI	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	CTTAGTGGCATTAATTCGDAATCC	144.9217938
BD42645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81895614	WGS84	BD42645	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42645_Rhar_Sim2018_COI	ADV-19	1	BD42645_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42645	Sample	BD42645	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-19	COI	45	BD42645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-19	CTGCTGCTATTGCTCATG	144.9217938
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42647_Pper_Dia2013_COI	ADV-21	1	BD42647_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-21	COI	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	CTTAGTGGCATTAATTCGDAATCC	144.9156239
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81969679	WGS84	BD42647	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42647_Vgib_Wil2025_COI	ADV-21	0	BD42647_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-21	COI	45	BD42647	1.00E-05	Nil	Australia	ADV-21	CCCCTCTTTACAAGTTCTTTAGGGT	144.9156239
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81919597	WGS84	BD42648	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.15	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42648_Asen_Wil2025_COI	ADV-22	0	BD42648_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Present	Sample	Asen_Wil2025_COI	ADV-22	COI	45	BD42648	1.00E-05	Nil	Australia	ADV-22	GTTGTTGTTACAACACATGCTCTTTTG	144.9153674
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42648_Cjap_Sim2018_COI	ADV-22	1	BD42648_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-22	COI	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9153674
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42648_Esin_And2018_Cyb	ADV-22	1	BD42648_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-22	Cytb	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	ACCCCTCCTCATATCCAACCA	144.9153674
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42648_Mjap_Gib2022_COI	ADV-22	1	BD42648_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-22	COI	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	AATTTAATTGTGACTAGGCATGGTCTG	144.9153674
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42649_Hsan_Wil2021_COI	ADV-23	1	BD42649_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-23	COI	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	CCTGGGCCGGTATAGTAGGT	144.9154956
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42649_Mros_Gib2022_COI	ADV-23	1	BD42649_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-23	COI	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9154956
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42649_Pvir_Dia2013_COI	ADV-23	1	BD42649_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-23	COI	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	CTTAGTGGCATTAATTCGDAATCC	144.9154956
BD42649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81944634	WGS84	BD42649	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42649_Rhar_Sim2018_COI	ADV-23	1	BD42649_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42649	Sample	BD42649	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-23	COI	45	BD42649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-23	CTGCTGCTATTGCTCATG	144.9154956
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42650_Pper_Dia2013_COI	ADV-24	1	BD42650_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-24	COI	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	CTTAGTGGCATTAATTCGDAATCC	144.9157521
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81994715	WGS84	BD42650	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42650_Vgib_Wil2025_COI	ADV-24	0	BD42650_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-24	COI	45	BD42650	1.00E-05	Nil	Australia	ADV-24	CCCCTCTTTACAAGTTCTTTAGGGT	144.9157521
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8201976	WGS84	BD42651	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.759	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42651_Asen_Wil2025_COI	ADV-25	0	BD42651_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Present	Sample	Asen_Wil2025_COI	ADV-25	COI	45	BD42651	1.00E-05	Nil	Australia	ADV-25	GTTGTTGTTACAACACATGCTCTTTTG	144.9158804
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42651_Cjap_Sim2018_COI	ADV-25	1	BD42651_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-25	COI	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9158804
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42651_Esin_And2018_Cyb	ADV-25	1	BD42651_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-25	Cytb	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	ACCCCTCCTCATATCCAACCA	144.9158804
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42651_Mjap_Gib2022_COI	ADV-25	1	BD42651_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-25	COI	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	AATTTAATTGTGACTAGGCATGGTCTG	144.9158804
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42652_Hsan_Wil2021_COI	ADV-30	1	BD42652_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-30	COI	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	CCTGGGCCGGTATAGTAGGT	144.906424
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42652_Mros_Gib2022_COI	ADV-30	1	BD42652_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-30	COI	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.906424
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42652_Pvir_Dia2013_COI	ADV-30	1	BD42652_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-30	COI	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	CTTAGTGGCATTAATTCGDAATCC	144.906424
BD42652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84537488	WGS84	BD42652	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42652_Rhar_Sim2018_COI	ADV-30	1	BD42652_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42652	Sample	BD42652	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-30	COI	45	BD42652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-30	CTGCTGCTATTGCTCATG	144.906424
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42654_Pper_Dia2013_COI	ADV-28	1	BD42654_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-28	COI	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	CTTAGTGGCATTAATTCGDAATCC	144.9073504
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84503161	WGS84	BD42654	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42654_Vgib_Wil2025_COI	ADV-28	0	BD42654_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-28	COI	45	BD42654	1.00E-05	Nil	Australia	ADV-28	CCCCTCTTTACAAGTTCTTTAGGGT	144.9073504
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8449172	WGS84	BD42655	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42655_Asen_Wil2025_COI	ADV-27	0	BD42655_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-27	COI	45	BD42655	1.00E-05	Nil	Australia	ADV-27	GTTGTTGTTACAACACATGCTCTTTTG	144.9076592
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42655_Cjap_Sim2018_COI	ADV-27	1	BD42655_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-27	COI	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9076592
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42655_Esin_And2018_Cyb	ADV-27	1	BD42655_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-27	Cytb	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	ACCCCTCCTCATATCCAACCA	144.9076592
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42655_Mjap_Gib2022_COI	ADV-27	1	BD42655_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-27	COI	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	AATTTAATTGTGACTAGGCATGGTCTG	144.9076592
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42656_Hsan_Wil2021_COI	ADV-26	1	BD42656_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-26	COI	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	CCTGGGCCGGTATAGTAGGT	144.9070416
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42656_Mros_Gib2022_COI	ADV-26	1	BD42656_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-26	COI	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9070416
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42656_Pvir_Dia2013_COI	ADV-26	1	BD42656_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-26	COI	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	CTTAGTGGCATTAATTCGDAATCC	144.9070416
BD42656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84514602	WGS84	BD42656	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42656_Rhar_Sim2018_COI	ADV-26	1	BD42656_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42656	Sample	BD42656	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-26	COI	45	BD42656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-26	CTGCTGCTATTGCTCATG	144.9070416
BD44107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55408	WGS84	BD44107	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44107_Asen_Wil2025_COI	ESAV-211	0	BD44107_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44107	Sample	BD44107	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-211	COI	45	BD44107	1.00E-05	Nil	Australia	ESAV-211	GTTGTTGTTACAACACATGCTCTTTTG	147.8808
BD44107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55408	WGS84	BD44107	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44107_Mjap_Gib2022_COI	ESAV-211	1	BD44107_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44107	Sample	BD44107	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-211	COI	45	BD44107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-211	AATTTAATTGTGACTAGGCATGGTCTG	147.8808
BD44108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55406	WGS84	BD44108	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44108_Mros_Gib2022_COI	ESAV-212	1	BD44108_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44108	Sample	BD44108	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-212	COI	45	BD44108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-212	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87962
BD44110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55502	WGS84	BD44110	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44110_Asen_Wil2025_COI	ESAV-214	0	BD44110_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44110	Sample	BD44110	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-214	COI	45	BD44110	1.00E-05	Nil	Australia	ESAV-214	GTTGTTGTTACAACACATGCTCTTTTG	147.87684
BD44110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55502	WGS84	BD44110	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	31.09	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44110_Mjap_Gib2022_COI	ESAV-214	1	BD44110_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44110	Sample	BD44110	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-214	COI	45	BD44110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-214	AATTTAATTGTGACTAGGCATGGTCTG	147.87684
BD44111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55539	WGS84	BD44111	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.57	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44111_Mros_Gib2022_COI	ESAV-215	1	BD44111_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44111	Sample	BD44111	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-215	COI	45	BD44111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-215	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87551
BD44114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.5486	WGS84	BD44114	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44114_Asen_Wil2025_COI	ESAV-218	0	BD44114_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44114	Sample	BD44114	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-218	COI	45	BD44114	1.00E-05	Nil	Australia	ESAV-218	GTTGTTGTTACAACACATGCTCTTTTG	147.92963
BD44114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5486	WGS84	BD44114	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	31.372	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44114_Mjap_Gib2022_COI	ESAV-218	1	BD44114_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44114	Sample	BD44114	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-218	COI	45	BD44114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-218	AATTTAATTGTGACTAGGCATGGTCTG	147.92963
BD44115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54758	WGS84	BD44115	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.737	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44115_Mros_Gib2022_COI	ESAV-219	1	BD44115_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44115	Sample	BD44115	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-219	COI	45	BD44115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-219	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.92946
BD44118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51625	WGS84	BD44118	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	26.01	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44118_Mjap_Gib2022_COI	ESAV-223	1	BD44118_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44118	Sample	BD44118	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-223	COI	45	BD44118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-223	AATTTAATTGTGACTAGGCATGGTCTG	147.91345
BD44119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15041	WGS84	BD44119	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.512	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44119_Mros_Gib2022_COI	ESAV-204	1	BD44119_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44119	Sample	BD44119	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-204	COI	45	BD44119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-204	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28248
BD44120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.14877	WGS84	BD44120	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	33.366	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44120_Vgib_Wil2025_COI	ESAV-203	0	BD44120_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44120	Sample	BD44120	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-203	COI	45	BD44120	1.00E-05	Nil	Australia	ESAV-203	CCCCTCTTTACAAGTTCTTTAGGGT	147.28186
BD44121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55372	WGS84	BD44121	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44121_Mjap_Gib2022_COI	ESAV-227	1	BD44121_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44121	Sample	BD44121	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-227	COI	45	BD44121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-227	AATTTAATTGTGACTAGGCATGGTCTG	147.88807
BD44122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55356	WGS84	BD44122	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44122_Mros_Gib2022_COI	ESAV-226	1	BD44122_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44122	Sample	BD44122	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-226	COI	45	BD44122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-226	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.89482
BD44125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15187	WGS84	BD44125	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44125_Asen_Wil2025_COI	ESAV-205	0	BD44125_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44125	Sample	BD44125	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-205	COI	45	BD44125	1.00E-05	Nil	Australia	ESAV-205	GTTGTTGTTACAACACATGCTCTTTTG	147.28325
BD44125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15187	WGS84	BD44125	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44125_Mjap_Gib2022_COI	ESAV-205	1	BD44125_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44125	Sample	BD44125	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-205	COI	45	BD44125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-205	AATTTAATTGTGACTAGGCATGGTCTG	147.28325
BD44126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15334	WGS84	BD44126	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	36.617	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44126_Mros_Gib2022_COI	ESAV-206	1	BD44126_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44126	Sample	BD44126	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-206	COI	45	BD44126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-206	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28351
BD44128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1557	WGS84	BD44128	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.253	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44128_Vgib_Wil2025_COI	ESAV-208	0	BD44128_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44128	Sample	BD44128	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-208	COI	45	BD44128	1.00E-05	Nil	Australia	ESAV-208	CCCCTCTTTACAAGTTCTTTAGGGT	147.28187
BD44129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15662	WGS84	BD44129	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.133	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44129_Asen_Wil2025_COI	ESAV-209	0	BD44129_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44129	Sample	BD44129	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-209	COI	45	BD44129	1.00E-05	Nil	Australia	ESAV-209	GTTGTTGTTACAACACATGCTCTTTTG	147.28031
BD44129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15662	WGS84	BD44129	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44129_Mjap_Gib2022_COI	ESAV-209	1	BD44129_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44129	Sample	BD44129	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-209	COI	45	BD44129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-209	AATTTAATTGTGACTAGGCATGGTCTG	147.28031
BD44131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.154	WGS84	BD44131	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.375	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44131_Vgib_Wil2025_COI	ESAV-191	0	BD44131_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44131	Sample	BD44131	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-191	COI	45	BD44131	1.00E-05	Nil	Australia	ESAV-191	CCCCTCTTTACAAGTTCTTTAGGGT	147.28375
BD44132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1546	WGS84	BD44132	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44132_Asen_Wil2025_COI	ESAV-193	0	BD44132_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44132	Sample	BD44132	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-193	COI	45	BD44132	1.00E-05	Nil	Australia	ESAV-193	GTTGTTGTTACAACACATGCTCTTTTG	147.28539
BD44132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1546	WGS84	BD44132	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44132_Mjap_Gib2022_COI	ESAV-193	1	BD44132_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44132	Sample	BD44132	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-193	COI	45	BD44132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-193	AATTTAATTGTGACTAGGCATGGTCTG	147.28539
BD44133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15661	WGS84	BD44133	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	26.691	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44133_Mros_Gib2022_COI	ESAV-194	1	BD44133_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44133	Sample	BD44133	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-194	COI	45	BD44133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-194	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28693
BD44135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15543	WGS84	BD44135	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.969	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44135_Vgib_Wil2025_COI	ESAV-197	0	BD44135_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44135	Sample	BD44135	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-197	COI	45	BD44135	1.00E-05	Nil	Australia	ESAV-197	CCCCTCTTTACAAGTTCTTTAGGGT	147.29045
BD44136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15645	WGS84	BD44136	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.319	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44136_Asen_Wil2025_COI	ESAV-199	0	BD44136_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44136	Sample	BD44136	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-199	COI	45	BD44136	1.00E-05	Nil	Australia	ESAV-199	GTTGTTGTTACAACACATGCTCTTTTG	147.29038
BD44136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15645	WGS84	BD44136	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44136_Mjap_Gib2022_COI	ESAV-199	1	BD44136_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44136	Sample	BD44136	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-199	COI	45	BD44136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-199	AATTTAATTGTGACTAGGCATGGTCTG	147.29038
BD44137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15657	WGS84	BD44137	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	26.914	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44137_Mros_Gib2022_COI	ESAV-201	1	BD44137_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44137	Sample	BD44137	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-201	COI	45	BD44137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-201	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28881
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44139_Pper_Dia2013_COI	ESAV-002	1	BD44139_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-002	COI	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	CTTAGTGGCATTAATTCGDAATCC	147.24847
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15882	WGS84	BD44139	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	34.856	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44139_Vgib_Wil2025_COI	ESAV-002	0	BD44139_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-002	COI	45	BD44139	1.00E-05	Nil	Australia	ESAV-002	CCCCTCTTTACAAGTTCTTTAGGGT	147.24847
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44140_Hsan_Wil2021_COI	ESAV-003	1	BD44140_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-003	COI	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	CCTGGGCCGGTATAGTAGGT	147.25183
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	26.71	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44140_Mros_Gib2022_COI	ESAV-003	1	BD44140_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-003	COI	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.25183
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44140_Pvir_Dia2013_COI	ESAV-003	1	BD44140_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-003	COI	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	CTTAGTGGCATTAATTCGDAATCC	147.25183
BD44140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44140	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44140_Rhar_Sim2018_COI	ESAV-003	1	BD44140_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44140	Sample	BD44140	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-003	COI	45	BD44140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-003	CTGCTGCTATTGCTCATG	147.25183
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44142_Pper_Dia2013_COI	ESAV-005	1	BD44142_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-005	COI	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	CTTAGTGGCATTAATTCGDAATCC	147.25847
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1605	WGS84	BD44142	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	32.622	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44142_Vgib_Wil2025_COI	ESAV-005	0	BD44142_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-005	COI	45	BD44142	1.00E-05	Nil	Australia	ESAV-005	CCCCTCTTTACAAGTTCTTTAGGGT	147.25847
BD44143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1618	WGS84	BD44143	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.334	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44143_Asen_Wil2025_COI	ESAV-006	0	BD44143_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44143	Sample	BD44143	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-006	COI	45	BD44143	1.00E-05	Nil	Australia	ESAV-006	GTTGTTGTTACAACACATGCTCTTTTG	147.26127
BD44143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1618	WGS84	BD44143	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44143_Mjap_Gib2022_COI	ESAV-006	1	BD44143_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44143	Sample	BD44143	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-006	COI	45	BD44143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-006	AATTTAATTGTGACTAGGCATGGTCTG	147.26127
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44144_Hsan_Wil2021_COI	ESAV-007	1	BD44144_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-007	COI	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	CCTGGGCCGGTATAGTAGGT	147.25872
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.107	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44144_Mros_Gib2022_COI	ESAV-007	1	BD44144_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-007	COI	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.25872
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44144_Pvir_Dia2013_COI	ESAV-007	1	BD44144_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-007	COI	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	CTTAGTGGCATTAATTCGDAATCC	147.25872
BD44144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.16088	WGS84	BD44144	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44144_Rhar_Sim2018_COI	ESAV-007	1	BD44144_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44144	Sample	BD44144	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-007	COI	45	BD44144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-007	CTGCTGCTATTGCTCATG	147.25872
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44146_Pper_Dia2013_COI	ESAV-009	1	BD44146_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-009	COI	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	CTTAGTGGCATTAATTCGDAATCC	147.28775
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15595	WGS84	BD44146	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.635	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44146_Vgib_Wil2025_COI	ESAV-009	0	BD44146_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-009	COI	45	BD44146	1.00E-05	Nil	Australia	ESAV-009	CCCCTCTTTACAAGTTCTTTAGGGT	147.28775
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15468	WGS84	BD44147	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	39.171	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44147_Asen_Wil2025_COI	ESAV-010	0	BD44147_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-010	COI	45	BD44147	1.00E-05	Nil	Australia	ESAV-010	GTTGTTGTTACAACACATGCTCTTTTG	147.28753
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44147_Cjap_Sim2018_COI	ESAV-010	1	BD44147_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-010	COI	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.28753
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44147_Esin_And2018_Cyb	ESAV-010	1	BD44147_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-010	Cytb	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	ACCCCTCCTCATATCCAACCA	147.28753
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44147_Mjap_Gib2022_COI	ESAV-010	1	BD44147_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-010	COI	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	AATTTAATTGTGACTAGGCATGGTCTG	147.28753
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44148_Hsan_Wil2021_COI	ESAV-011	1	BD44148_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-011	COI	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	CCTGGGCCGGTATAGTAGGT	147.28832
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	26.952	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44148_Mros_Gib2022_COI	ESAV-011	1	BD44148_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-011	COI	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28832
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44148_Pvir_Dia2013_COI	ESAV-011	1	BD44148_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-011	COI	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	CTTAGTGGCATTAATTCGDAATCC	147.28832
BD44148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15335	WGS84	BD44148	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44148_Rhar_Sim2018_COI	ESAV-011	1	BD44148_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44148	Sample	BD44148	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-011	COI	45	BD44148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-011	CTGCTGCTATTGCTCATG	147.28832
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15132	WGS84	BD44150	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44150_Asen_Wil2025_COI	ESAV-013	0	BD44150_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-013	COI	45	BD44150	1.00E-05	Nil	Australia	ESAV-013	GTTGTTGTTACAACACATGCTCTTTTG	147.28998
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44150_Cjap_Sim2018_COI	ESAV-013	1	BD44150_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-013	COI	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.28998
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44150_Esin_And2018_Cyb	ESAV-013	1	BD44150_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-013	Cytb	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	ACCCCTCCTCATATCCAACCA	147.28998
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44150_Mjap_Gib2022_COI	ESAV-013	1	BD44150_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-013	COI	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	AATTTAATTGTGACTAGGCATGGTCTG	147.28998
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44151_Hsan_Wil2021_COI	ESAV-014	1	BD44151_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-014	COI	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	CCTGGGCCGGTATAGTAGGT	147.2887
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.881	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44151_Mros_Gib2022_COI	ESAV-014	1	BD44151_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-014	COI	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.2887
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44151_Pvir_Dia2013_COI	ESAV-014	1	BD44151_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-014	COI	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	CTTAGTGGCATTAATTCGDAATCC	147.2887
BD44151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15225	WGS84	BD44151	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44151_Rhar_Sim2018_COI	ESAV-014	1	BD44151_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44151	Sample	BD44151	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-014	COI	45	BD44151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-014	CTGCTGCTATTGCTCATG	147.2887
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44153_Pper_Dia2013_COI	ESAV-016	1	BD44153_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-016	COI	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	CTTAGTGGCATTAATTCGDAATCC	147.88314
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44154_Cjap_Sim2018_COI	ESAV-018	1	BD44154_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-018	COI	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.8818
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44154_Esin_And2018_Cyb	ESAV-018	1	BD44154_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-018	Cytb	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	ACCCCTCCTCATATCCAACCA	147.8818
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	32.655	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44154_Mjap_Gib2022_COI	ESAV-018	1	BD44154_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-018	COI	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	AATTTAATTGTGACTAGGCATGGTCTG	147.8818
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44155_Hsan_Wil2021_COI	ESAV-019	1	BD44155_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-019	COI	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	CCTGGGCCGGTATAGTAGGT	147.88082
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.671	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44155_Mros_Gib2022_COI	ESAV-019	1	BD44155_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-019	COI	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.88082
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44155_Pvir_Dia2013_COI	ESAV-019	1	BD44155_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-019	COI	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	CTTAGTGGCATTAATTCGDAATCC	147.88082
BD44155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55407	WGS84	BD44155	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44155_Rhar_Sim2018_COI	ESAV-019	1	BD44155_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44155	Sample	BD44155	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-019	COI	45	BD44155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-019	CTGCTGCTATTGCTCATG	147.88082
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44157_Pper_Dia2013_COI	ESAV-022	1	BD44157_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-022	COI	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	CTTAGTGGCATTAATTCGDAATCC	147.8773
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44158_Cjap_Sim2018_COI	ESAV-025	1	BD44158_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-025	COI	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87623
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44158_Esin_And2018_Cyb	ESAV-025	1	BD44158_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-025	Cytb	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	ACCCCTCCTCATATCCAACCA	147.87623
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	33.723	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44158_Mjap_Gib2022_COI	ESAV-025	1	BD44158_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-025	COI	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	AATTTAATTGTGACTAGGCATGGTCTG	147.87623
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44159_Hsan_Wil2021_COI	ESAV-028	1	BD44159_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-028	COI	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	CCTGGGCCGGTATAGTAGGT	147.87426
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.443	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44159_Mros_Gib2022_COI	ESAV-028	1	BD44159_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-028	COI	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87426
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44159_Pvir_Dia2013_COI	ESAV-028	1	BD44159_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-028	COI	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	CTTAGTGGCATTAATTCGDAATCC	147.87426
BD44159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5574	WGS84	BD44159	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44159_Rhar_Sim2018_COI	ESAV-028	1	BD44159_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44159	Sample	BD44159	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-028	COI	45	BD44159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-028	CTGCTGCTATTGCTCATG	147.87426
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44160_Pper_Dia2013_COI	ESAV-030	1	BD44160_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-030	COI	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	CTTAGTGGCATTAATTCGDAATCC	147.87273
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44161_Cjap_Sim2018_COI	ESAV-032	1	BD44161_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-032	COI	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.92961
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44161_Esin_And2018_Cyb	ESAV-032	1	BD44161_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-032	Cytb	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	ACCCCTCCTCATATCCAACCA	147.92961
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	36.79	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44161_Mjap_Gib2022_COI	ESAV-032	1	BD44161_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-032	COI	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	AATTTAATTGTGACTAGGCATGGTCTG	147.92961
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44162_Hsan_Wil2021_COI	ESAV-033	1	BD44162_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-033	COI	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	CCTGGGCCGGTATAGTAGGT	147.92926
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44162_Mros_Gib2022_COI	ESAV-033	1	BD44162_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-033	COI	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.92926
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44162_Pvir_Dia2013_COI	ESAV-033	1	BD44162_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-033	COI	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	CTTAGTGGCATTAATTCGDAATCC	147.92926
BD44162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54672	WGS84	BD44162	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44162_Rhar_Sim2018_COI	ESAV-033	1	BD44162_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44162	Sample	BD44162	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-033	COI	45	BD44162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-033	CTGCTGCTATTGCTCATG	147.92926
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44164_Pper_Dia2013_COI	ESAV-035	1	BD44164_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-035	COI	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	CTTAGTGGCATTAATTCGDAATCC	147.9142
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44165_Cjap_Sim2018_COI	ESAV-036	1	BD44165_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-036	COI	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.91501
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44165_Esin_And2018_Cyb	ESAV-036	1	BD44165_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-036	Cytb	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	ACCCCTCCTCATATCCAACCA	147.91501
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	35.056	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44165_Mjap_Gib2022_COI	ESAV-036	1	BD44165_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-036	COI	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	AATTTAATTGTGACTAGGCATGGTCTG	147.91501
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44166_Hsan_Wil2021_COI	ESAV-037	1	BD44166_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-037	COI	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	CCTGGGCCGGTATAGTAGGT	147.9156
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	30.368	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44166_Mros_Gib2022_COI	ESAV-037	1	BD44166_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-037	COI	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.9156
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44166_Pvir_Dia2013_COI	ESAV-037	1	BD44166_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-037	COI	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	CTTAGTGGCATTAATTCGDAATCC	147.9156
BD44166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5101	WGS84	BD44166	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44166_Rhar_Sim2018_COI	ESAV-037	1	BD44166_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44166	Sample	BD44166	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-037	COI	45	BD44166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-037	CTGCTGCTATTGCTCATG	147.9156
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD44708_Aamu_Bax2006_COI	POR-B23	1	BD44708_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	GCACAACCGGGATCTTTACTTC	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD44708_Pcan_Bot2011_IGS	POR-B23	1	BD44708_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B23	IGS	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	CGTAATCCTCAGTACTGGCTA	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44708_Pper_Dia2013_COI	POR-B23	1	BD44708_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	CTTAGTGGCATTAATTCGDAATCC	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD44708_Sspa_Oph2007_COI	POR-B23	1	BD44708_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	GAAGGCCTTCCGATGATC	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.503	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD44708_Upin_Bot2011_COI	POR-B23	1	BD44708_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Present	Sample	Upin_Bot2011_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	CTTTAATTACAGCGTTTTTATTGTTGT	141.61277
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44709_Cjap_Sim2018_COI	POR-B24	1	BD44709_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44709_Esin_And2018_Cyb	POR-B24	1	BD44709_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B24	Cytb	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	ACCCCTCCTCATATCCAACCA	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD44709_Mgig_Bot2012_COI	POR-B24	1	BD44709_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	TCTTATTCGTTGGAGACTTTATAACCCT	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44709_Mjap_Gib2022_COI	POR-B24	1	BD44709_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	AATTTAATTGTGACTAGGCATGGTCTG	141.61416
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD44711_Aamu_Bax2006_COI	POR-B26	1	BD44711_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	GCACAACCGGGATCTTTACTTC	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD44711_Pcan_Bot2011_IGS	POR-B26	1	BD44711_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B26	IGS	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	CGTAATCCTCAGTACTGGCTA	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44711_Pper_Dia2013_COI	POR-B26	1	BD44711_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	CTTAGTGGCATTAATTCGDAATCC	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD44711_Sspa_Oph2007_COI	POR-B26	1	BD44711_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	GAAGGCCTTCCGATGATC	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.203	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD44711_Upin_Bot2011_COI	POR-B26	1	BD44711_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Present	Sample	Upin_Bot2011_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	CTTTAATTACAGCGTTTTTATTGTTGT	141.61693
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44712_Cjap_Sim2018_COI	POR-B27	1	BD44712_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44712_Esin_And2018_Cyb	POR-B27	1	BD44712_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B27	Cytb	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	ACCCCTCCTCATATCCAACCA	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD44712_Mgig_Bot2012_COI	POR-B27	1	BD44712_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	TCTTATTCGTTGGAGACTTTATAACCCT	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44712_Mjap_Gib2022_COI	POR-B27	1	BD44712_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	AATTTAATTGTGACTAGGCATGGTCTG	141.61831
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD44713_Cmae_Bot2011_COI	POR-B28	1	BD44713_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	ATGAACAGTCTATCCTCCTTTAG	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44713_Hsan_Wil2021_COI	POR-B28	1	BD44713_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	CCTGGGCCGGTATAGTAGGT	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44713_Mros_Gib2022_COI	POR-B28	1	BD44713_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD44713_Msal_Bot2012_COI	POR-B28	1	BD44713_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	GYTAGTTCCRATGATGTTAGCTG	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD44713_Mstr_Wil2021_COI	POR-B28	1	BD44713_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	GTAGTGGTAACTACTCATGCTTTA	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44713_Pvir_Dia2013_COI	POR-B28	1	BD44713_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	CTTAGTGGCATTAATTCGDAATCC	141.61972
BD44713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34655	WGS84	BD44713	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44713_Rhar_Sim2018_COI	POR-B28	1	BD44713_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44713	Sample	BD44713	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B28	COI	45	BD44713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B28	CTGCTGCTATTGCTCATG	141.61972
BD47586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-32.14609	WGS84	BD47586	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47586_Esin_And2018_Cyb	THE-B37	1	BD47586_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47586	Sample	BD47586	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B37	Cytb	45	BD47586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B37	ACCCCTCCTCATATCCAACCA	133.64044
BD47586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-32.14609	WGS84	BD47586	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47586_Mjap_Gib2022_COI	THE-B37	1	BD47586_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47586	Sample	BD47586	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B37	COI	45	BD47586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B37	AATTTAATTGTGACTAGGCATGGTCTG	133.64044
BD47592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8198	WGS84	BD47592	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47592_Pcan_Bot2011_IGS	GC30	1	BD47592_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47592	Sample	BD47592	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC30	IGS	45	BD47592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC30	CGTAATCCTCAGTACTGGCTA	151.24001
BD47592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8198	WGS84	BD47592	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47592_Vgib_Wil2025_COI	GC30	1	BD47592_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47592	Sample	BD47592	targeted	Absent	Sample	Vgib_Wil2025_COI	GC30	COI	45	BD47592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC30	CCCCTCTTTACAAGTTCTTTAGGGT	151.24001
BD47593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82665	WGS84	BD47593	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47593_Asen_Wil2025_COI	GC39	1	BD47593_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47593	Sample	BD47593	targeted	Absent	Sample	Asen_Wil2025_COI	GC39	COI	45	BD47593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC39	GTTGTTGTTACAACACATGCTCTTTTG	151.24658
BD47593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82665	WGS84	BD47593	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47593_Esin_And2018_Cyb	GC39	1	BD47593_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47593	Sample	BD47593	targeted	Absent	Sample	Esin_And2018_Cyb	GC39	Cytb	45	BD47593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC39	ACCCCTCCTCATATCCAACCA	151.24658
BD47593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82665	WGS84	BD47593	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47593_Mjap_Gib2022_COI	GC39	1	BD47593_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47593	Sample	BD47593	targeted	Absent	Sample	Mjap_Gib2022_COI	GC39	COI	45	BD47593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC39	AATTTAATTGTGACTAGGCATGGTCTG	151.24658
BD47597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81301	WGS84	BD47597	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47597_Esin_And2018_Cyb	ESAV-083	1	BD47597_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47597	Sample	BD47597	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-083	Cytb	45	BD47597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-083	ACCCCTCCTCATATCCAACCA	138.46399
BD47597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81301	WGS84	BD47597	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47597_Mjap_Gib2022_COI	ESAV-083	1	BD47597_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47597	Sample	BD47597	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-083	COI	45	BD47597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-083	AATTTAATTGTGACTAGGCATGGTCTG	138.46399
BD47600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82069	WGS84	BD47600	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47600_Pcan_Bot2011_IGS	GB100	1	BD47600_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47600	Sample	BD47600	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB100	IGS	45	BD47600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB100	CGTAATCCTCAGTACTGGCTA	151.24147
BD47600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82069	WGS84	BD47600	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47600_Pper_Dia2013_COI	GB100	1	BD47600_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47600	Sample	BD47600	targeted	Absent	Sample	Pper_Dia2013_COI	GB100	COI	45	BD47600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB100	CTTAGTGGCATTAATTCGDAATCC	151.24147
BD47600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82069	WGS84	BD47600	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47600_Vgib_Wil2025_COI	GB100	1	BD47600_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47600	Sample	BD47600	targeted	Absent	Sample	Vgib_Wil2025_COI	GB100	COI	45	BD47600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB100	CCCCTCTTTACAAGTTCTTTAGGGT	151.24147
BD47601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80641	WGS84	BD47601	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47601_Esin_And2018_Cyb	GC106	1	BD47601_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47601	Sample	BD47601	targeted	Absent	Sample	Esin_And2018_Cyb	GC106	Cytb	45	BD47601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC106	ACCCCTCCTCATATCCAACCA	151.22058
BD47601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80641	WGS84	BD47601	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47601_Mjap_Gib2022_COI	GC106	1	BD47601_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47601	Sample	BD47601	targeted	Absent	Sample	Mjap_Gib2022_COI	GC106	COI	45	BD47601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC106	AATTTAATTGTGACTAGGCATGGTCTG	151.22058
BD47605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86448	WGS84	BD47605	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47605_Esin_And2018_Cyb	ESAV-122	1	BD47605_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47605	Sample	BD47605	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-122	Cytb	45	BD47605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-122	ACCCCTCCTCATATCCAACCA	138.46828
BD47605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86448	WGS84	BD47605	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47605_Mjap_Gib2022_COI	ESAV-122	1	BD47605_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47605	Sample	BD47605	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-122	COI	45	BD47605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-122	AATTTAATTGTGACTAGGCATGGTCTG	138.46828
BD47609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81945	WGS84	BD47609	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47609_Esin_And2018_Cyb	ESAV-074	1	BD47609_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47609	Sample	BD47609	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-074	Cytb	45	BD47609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-074	ACCCCTCCTCATATCCAACCA	138.46328
BD47609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81945	WGS84	BD47609	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47609_Mjap_Gib2022_COI	ESAV-074	1	BD47609_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47609	Sample	BD47609	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-074	COI	45	BD47609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-074	AATTTAATTGTGACTAGGCATGGTCTG	138.46328
BD47611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.79115	WGS84	BD47611	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47611_Vgib_Wil2025_COI	GC99	1	BD47611_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47611	Sample	BD47611	targeted	Absent	Sample	Vgib_Wil2025_COI	GC99	COI	45	BD47611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC99	CCCCTCTTTACAAGTTCTTTAGGGT	151.17999
BD47612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.85544	WGS84	BD47612	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47612_Esin_And2018_Cyb	GC13	1	BD47612_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47612	Sample	BD47612	targeted	Absent	Sample	Esin_And2018_Cyb	GC13	Cytb	45	BD47612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC13	ACCCCTCCTCATATCCAACCA	151.32222
BD47612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.85544	WGS84	BD47612	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47612_Mjap_Gib2022_COI	GC13	1	BD47612_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47612	Sample	BD47612	targeted	Absent	Sample	Mjap_Gib2022_COI	GC13	COI	45	BD47612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC13	AATTTAATTGTGACTAGGCATGGTCTG	151.32222
BD47615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01932	WGS84	BD47615	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47615_Pcan_Bot2011_IGS	ESAV-234	1	BD47615_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47615	Sample	BD47615	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-234	IGS	45	BD47615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-234	CGTAATCCTCAGTACTGGCTA	137.7696
BD47615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01932	WGS84	BD47615	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47615_Vgib_Wil2025_COI	ESAV-234	1	BD47615_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47615	Sample	BD47615	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-234	COI	45	BD47615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-234	CCCCTCTTTACAAGTTCTTTAGGGT	137.7696
BD47616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.71821	WGS84	BD47616	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47616_Esin_And2018_Cyb	ESAV-189	1	BD47616_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47616	Sample	BD47616	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-189	Cytb	45	BD47616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-189	ACCCCTCCTCATATCCAACCA	135.88066
BD47616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.71821	WGS84	BD47616	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	29/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47616_Mjap_Gib2022_COI	ESAV-189	1	BD47616_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47616	Sample	BD47616	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-189	COI	45	BD47616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-189	AATTTAATTGTGACTAGGCATGGTCTG	135.88066
BD47619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85579	WGS84	BD47619	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47619_Vgib_Wil2025_COI	ESAV-100	1	BD47619_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47619	Sample	BD47619	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-100	COI	45	BD47619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-100	CCCCTCTTTACAAGTTCTTTAGGGT	138.46577
BD47622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83492	WGS84	BD47622	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47622_Pcan_Bot2011_IGS	GB19	1	BD47622_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47622	Sample	BD47622	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB19	IGS	45	BD47622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB19	CGTAATCCTCAGTACTGGCTA	151.27091
BD47622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83492	WGS84	BD47622	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47622_Vgib_Wil2025_COI	GB19	1	BD47622_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47622	Sample	BD47622	targeted	Absent	Sample	Vgib_Wil2025_COI	GB19	COI	45	BD47622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB19	CCCCTCTTTACAAGTTCTTTAGGGT	151.27091
BD47623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82193	WGS84	BD47623	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47623_Esin_And2018_Cyb	GA21	1	BD47623_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47623	Sample	BD47623	targeted	Absent	Sample	Esin_And2018_Cyb	GA21	Cytb	45	BD47623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA21	ACCCCTCCTCATATCCAACCA	151.24014
BD47623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82193	WGS84	BD47623	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47623_Mjap_Gib2022_COI	GA21	1	BD47623_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47623	Sample	BD47623	targeted	Absent	Sample	Mjap_Gib2022_COI	GA21	COI	45	BD47623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA21	AATTTAATTGTGACTAGGCATGGTCTG	151.24014
BD47626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85478	WGS84	BD47626	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47626_Pcan_Bot2011_IGS	ESAV-131	1	BD47626_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47626	Sample	BD47626	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-131	IGS	45	BD47626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-131	CGTAATCCTCAGTACTGGCTA	138.47153
BD47626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85478	WGS84	BD47626	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47626_Pper_Dia2013_COI	ESAV-131	1	BD47626_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47626	Sample	BD47626	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-131	COI	45	BD47626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-131	CTTAGTGGCATTAATTCGDAATCC	138.47153
BD47626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85478	WGS84	BD47626	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47626_Vgib_Wil2025_COI	ESAV-131	1	BD47626_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47626	Sample	BD47626	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-131	COI	45	BD47626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-131	CCCCTCTTTACAAGTTCTTTAGGGT	138.47153
BD47627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81919	WGS84	BD47627	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47627_Esin_And2018_Cyb	GC68	1	BD47627_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47627	Sample	BD47627	targeted	Absent	Sample	Esin_And2018_Cyb	GC68	Cytb	45	BD47627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC68	ACCCCTCCTCATATCCAACCA	151.2429
BD47628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81925	WGS84	BD47628	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47628_Msal_Bot2012_COI	GC52	1	BD47628_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47628	Sample	BD47628	targeted	Absent	Sample	Msal_Bot2012_COI	GC52	COI	45	BD47628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC52	GYTAGTTCCRATGATGTTAGCTG	151.23972
BD47633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80231	WGS84	BD47633	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47633_Pcan_Bot2011_IGS	ESAV-176	1	BD47633_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47633	Sample	BD47633	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-176	IGS	45	BD47633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-176	CGTAATCCTCAGTACTGGCTA	138.48299
BD47633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.80231	WGS84	BD47633	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47633_Pper_Dia2013_COI	ESAV-176	1	BD47633_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47633	Sample	BD47633	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-176	COI	45	BD47633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-176	CTTAGTGGCATTAATTCGDAATCC	138.48299
BD47634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.95629	WGS84	BD47634	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47634_Esin_And2018_Cyb	KPT-A40	1	BD47634_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47634	Sample	BD47634	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A40	Cytb	45	BD47634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A40	ACCCCTCCTCATATCCAACCA	137.77862
BD47637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85887	WGS84	BD47637	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47637_Pcan_Bot2011_IGS	ESAV-127	1	BD47637_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47637	Sample	BD47637	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-127	IGS	45	BD47637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-127	CGTAATCCTCAGTACTGGCTA	138.47
BD47637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85887	WGS84	BD47637	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47637_Pper_Dia2013_COI	ESAV-127	1	BD47637_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47637	Sample	BD47637	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-127	COI	45	BD47637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-127	CTTAGTGGCATTAATTCGDAATCC	138.47
BD47637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85887	WGS84	BD47637	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47637_Vgib_Wil2025_COI	ESAV-127	1	BD47637_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47637	Sample	BD47637	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-127	COI	45	BD47637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-127	CCCCTCTTTACAAGTTCTTTAGGGT	138.47
BD47640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80515	WGS84	BD47640	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47640_Pcan_Bot2011_IGS	GC104	1	BD47640_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47640	Sample	BD47640	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC104	IGS	45	BD47640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC104	CGTAATCCTCAGTACTGGCTA	151.21764
BD47640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80515	WGS84	BD47640	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47640_Pper_Dia2013_COI	GC104	1	BD47640_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47640	Sample	BD47640	targeted	Absent	Sample	Pper_Dia2013_COI	GC104	COI	45	BD47640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC104	CTTAGTGGCATTAATTCGDAATCC	151.21764
BD47640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.80515	WGS84	BD47640	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47640_Vgib_Wil2025_COI	GC104	1	BD47640_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47640	Sample	BD47640	targeted	Absent	Sample	Vgib_Wil2025_COI	GC104	COI	45	BD47640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC104	CCCCTCTTTACAAGTTCTTTAGGGT	151.21764
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47642_Hsan_Wil2021_COI	GC28	1	BD47642_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Hsan_Wil2021_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	CCTGGGCCGGTATAGTAGGT	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47642_Mros_Gib2022_COI	GC28	1	BD47642_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Mros_Gib2022_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47642_Msal_Bot2012_COI	GC28	1	BD47642_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Msal_Bot2012_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	GYTAGTTCCRATGATGTTAGCTG	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47642_Pvir_Dia2013_COI	GC28	1	BD47642_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Pvir_Dia2013_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	CTTAGTGGCATTAATTCGDAATCC	151.23943
BD47642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81859	WGS84	BD47642	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47642_Rhar_Sim2018_COI	GC28	1	BD47642_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47642	Sample	BD47642	targeted	Absent	Sample	Rhar_Sim2018_COI	GC28	COI	45	BD47642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC28	CTGCTGCTATTGCTCATG	151.23943
BD47644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81938	WGS84	BD47644	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47644_Pcan_Bot2011_IGS	ESAV-160	1	BD47644_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47644	Sample	BD47644	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-160	IGS	45	BD47644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-160	CGTAATCCTCAGTACTGGCTA	138.48217
BD47644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81938	WGS84	BD47644	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47644_Pper_Dia2013_COI	ESAV-160	1	BD47644_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47644	Sample	BD47644	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-160	COI	45	BD47644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-160	CTTAGTGGCATTAATTCGDAATCC	138.48217
BD47644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81938	WGS84	BD47644	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47644_Vgib_Wil2025_COI	ESAV-160	1	BD47644_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47644	Sample	BD47644	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-160	COI	45	BD47644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-160	CCCCTCTTTACAAGTTCTTTAGGGT	138.48217
BD47645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82913	WGS84	BD47645	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47645_Esin_And2018_Cyb	ESAV-149	1	BD47645_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47645	Sample	BD47645	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-149	Cytb	45	BD47645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-149	ACCCCTCCTCATATCCAACCA	138.4748
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47648_Pcan_Bot2011_IGS	ESAV-046	1	BD47648_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-046	IGS	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	CGTAATCCTCAGTACTGGCTA	138.46418
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47648_Pper_Dia2013_COI	ESAV-046	1	BD47648_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-046	COI	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	CTTAGTGGCATTAATTCGDAATCC	138.46418
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47648_Vgib_Wil2025_COI	ESAV-046	1	BD47648_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-046	COI	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	CCCCTCTTTACAAGTTCTTTAGGGT	138.46418
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47649_Asen_Wil2025_COI	GC22	1	BD47649_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Asen_Wil2025_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	GTTGTTGTTACAACACATGCTCTTTTG	151.25658
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47649_Cjap_Sim2018_COI	GC22	1	BD47649_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Cjap_Sim2018_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25658
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47649_Esin_And2018_Cyb	GC22	1	BD47649_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Esin_And2018_Cyb	GC22	Cytb	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	ACCCCTCCTCATATCCAACCA	151.25658
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47649_Mjap_Gib2022_COI	GC22	1	BD47649_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Mjap_Gib2022_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	AATTTAATTGTGACTAGGCATGGTCTG	151.25658
BD47651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85676	WGS84	BD47651	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47651_Pcan_Bot2011_IGS	ESAV-129	1	BD47651_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47651	Sample	BD47651	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-129	IGS	45	BD47651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-129	CGTAATCCTCAGTACTGGCTA	138.47096
BD47651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85676	WGS84	BD47651	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47651_Pper_Dia2013_COI	ESAV-129	1	BD47651_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47651	Sample	BD47651	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-129	COI	45	BD47651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-129	CTTAGTGGCATTAATTCGDAATCC	138.47096
BD47651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85676	WGS84	BD47651	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47651_Vgib_Wil2025_COI	ESAV-129	1	BD47651_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47651	Sample	BD47651	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-129	COI	45	BD47651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-129	CCCCTCTTTACAAGTTCTTTAGGGT	138.47096
BD47655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.799	WGS84	BD47655	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47655_Pcan_Bot2011_IGS	ESAV-183	1	BD47655_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47655	Sample	BD47655	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-183	IGS	45	BD47655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-183	CGTAATCCTCAGTACTGGCTA	138.47835
BD47655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.799	WGS84	BD47655	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47655_Pper_Dia2013_COI	ESAV-183	1	BD47655_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47655	Sample	BD47655	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-183	COI	45	BD47655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-183	CTTAGTGGCATTAATTCGDAATCC	138.47835
BD47655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.799	WGS84	BD47655	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47655_Vgib_Wil2025_COI	ESAV-183	1	BD47655_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47655	Sample	BD47655	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-183	COI	45	BD47655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-183	CCCCTCTTTACAAGTTCTTTAGGGT	138.47835
BD47656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.79769	WGS84	BD47656	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47656_Esin_And2018_Cyb	ESAV-181	1	BD47656_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47656	Sample	BD47656	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-181	Cytb	45	BD47656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-181	ACCCCTCCTCATATCCAACCA	138.48051
BD47656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.79769	WGS84	BD47656	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47656_Mjap_Gib2022_COI	ESAV-181	1	BD47656_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47656	Sample	BD47656	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-181	COI	45	BD47656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-181	AATTTAATTGTGACTAGGCATGGTCTG	138.48051
BD47659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82743	WGS84	BD47659	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47659_Pcan_Bot2011_IGS	ESAV-151	1	BD47659_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47659	Sample	BD47659	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-151	IGS	45	BD47659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-151	CGTAATCCTCAGTACTGGCTA	138.47671
BD47659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82743	WGS84	BD47659	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47659_Pper_Dia2013_COI	ESAV-151	1	BD47659_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47659	Sample	BD47659	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-151	COI	45	BD47659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-151	CTTAGTGGCATTAATTCGDAATCC	138.47671
BD47659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82743	WGS84	BD47659	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47659_Vgib_Wil2025_COI	ESAV-151	1	BD47659_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47659	Sample	BD47659	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-151	COI	45	BD47659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-151	CCCCTCTTTACAAGTTCTTTAGGGT	138.47671
BD47662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83533	WGS84	BD47662	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47662_Pcan_Bot2011_IGS	GB17	1	BD47662_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47662	Sample	BD47662	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB17	IGS	45	BD47662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB17	CGTAATCCTCAGTACTGGCTA	151.27433
BD47662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83533	WGS84	BD47662	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47662_Pper_Dia2013_COI	GB17	1	BD47662_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47662	Sample	BD47662	targeted	Absent	Sample	Pper_Dia2013_COI	GB17	COI	45	BD47662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB17	CTTAGTGGCATTAATTCGDAATCC	151.27433
BD47662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83533	WGS84	BD47662	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47662_Vgib_Wil2025_COI	GB17	1	BD47662_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47662	Sample	BD47662	targeted	Absent	Sample	Vgib_Wil2025_COI	GB17	COI	45	BD47662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB17	CCCCTCTTTACAAGTTCTTTAGGGT	151.27433
BD47664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14747	WGS84	BD47664	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47664_Hsan_Wil2021_COI	THE-B38	1	BD47664_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47664	Sample	BD47664	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B38	COI	45	BD47664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B38	CCTGGGCCGGTATAGTAGGT	133.64081
BD47664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14747	WGS84	BD47664	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47664_Msal_Bot2012_COI	THE-B38	1	BD47664_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47664	Sample	BD47664	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B38	COI	45	BD47664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B38	GYTAGTTCCRATGATGTTAGCTG	133.64081
BD47664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-32.14747	WGS84	BD47664	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47664_Pvir_Dia2013_COI	THE-B38	1	BD47664_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47664	Sample	BD47664	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B38	COI	45	BD47664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B38	CTTAGTGGCATTAATTCGDAATCC	133.64081
BD47668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82949	WGS84	BD47668	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47668_Hsan_Wil2021_COI	ESAV-063	1	BD47668_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47668	Sample	BD47668	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-063	COI	45	BD47668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-063	CCTGGGCCGGTATAGTAGGT	138.46217
BD47668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82949	WGS84	BD47668	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47668_Msal_Bot2012_COI	ESAV-063	1	BD47668_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47668	Sample	BD47668	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-063	COI	45	BD47668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-063	GYTAGTTCCRATGATGTTAGCTG	138.46217
BD47668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82949	WGS84	BD47668	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47668_Pvir_Dia2013_COI	ESAV-063	1	BD47668_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47668	Sample	BD47668	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-063	COI	45	BD47668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-063	CTTAGTGGCATTAATTCGDAATCC	138.46217
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47671_Hsan_Wil2021_COI	GB67	1	BD47671_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Hsan_Wil2021_COI	GB67	COI	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	CCTGGGCCGGTATAGTAGGT	151.24434
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47671_Mros_Gib2022_COI	GB67	1	BD47671_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Mros_Gib2022_COI	GB67	COI	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.24434
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47671_Msal_Bot2012_COI	GB67	1	BD47671_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Msal_Bot2012_COI	GB67	COI	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	GYTAGTTCCRATGATGTTAGCTG	151.24434
BD47671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82356	WGS84	BD47671	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47671_Pvir_Dia2013_COI	GB67	1	BD47671_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47671	Sample	BD47671	targeted	Absent	Sample	Pvir_Dia2013_COI	GB67	COI	45	BD47671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB67	CTTAGTGGCATTAATTCGDAATCC	151.24434
BD47675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83782	WGS84	BD47675	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47675_Hsan_Wil2021_COI	ESAV-141	1	BD47675_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47675	Sample	BD47675	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-141	COI	45	BD47675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-141	CCTGGGCCGGTATAGTAGGT	138.47302
BD47675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83782	WGS84	BD47675	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47675_Msal_Bot2012_COI	ESAV-141	1	BD47675_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47675	Sample	BD47675	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-141	COI	45	BD47675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-141	GYTAGTTCCRATGATGTTAGCTG	138.47302
BD47675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83782	WGS84	BD47675	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47675_Pvir_Dia2013_COI	ESAV-141	1	BD47675_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47675	Sample	BD47675	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-141	COI	45	BD47675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-141	CTTAGTGGCATTAATTCGDAATCC	138.47302
BD47679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81853	WGS84	BD47679	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47679_Hsan_Wil2021_COI	ESAV-075	1	BD47679_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47679	Sample	BD47679	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-075	COI	45	BD47679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-075	CCTGGGCCGGTATAGTAGGT	138.46329
BD47679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81853	WGS84	BD47679	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47679_Msal_Bot2012_COI	ESAV-075	1	BD47679_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47679	Sample	BD47679	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-075	COI	45	BD47679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-075	GYTAGTTCCRATGATGTTAGCTG	138.46329
BD47679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81853	WGS84	BD47679	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47679_Pvir_Dia2013_COI	ESAV-075	1	BD47679_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47679	Sample	BD47679	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-075	COI	45	BD47679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-075	CTTAGTGGCATTAATTCGDAATCC	138.46329
BD47682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82627	WGS84	BD47682	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47682_Hsan_Wil2021_COI	GC58	1	BD47682_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47682	Sample	BD47682	targeted	Absent	Sample	Hsan_Wil2021_COI	GC58	COI	45	BD47682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC58	CCTGGGCCGGTATAGTAGGT	151.24725
BD47682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82627	WGS84	BD47682	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47682_Msal_Bot2012_COI	GC58	1	BD47682_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47682	Sample	BD47682	targeted	Absent	Sample	Msal_Bot2012_COI	GC58	COI	45	BD47682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC58	GYTAGTTCCRATGATGTTAGCTG	151.24725
BD47682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82627	WGS84	BD47682	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47682_Pvir_Dia2013_COI	GC58	1	BD47682_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47682	Sample	BD47682	targeted	Absent	Sample	Pvir_Dia2013_COI	GC58	COI	45	BD47682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC58	CTTAGTGGCATTAATTCGDAATCC	151.24725
BD47686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80334	WGS84	BD47686	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47686_Hsan_Wil2021_COI	ESAV-175	1	BD47686_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47686	Sample	BD47686	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-175	COI	45	BD47686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-175	CCTGGGCCGGTATAGTAGGT	138.48336
BD47686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80334	WGS84	BD47686	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47686_Msal_Bot2012_COI	ESAV-175	1	BD47686_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47686	Sample	BD47686	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-175	COI	45	BD47686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-175	GYTAGTTCCRATGATGTTAGCTG	138.48336
BD47686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80334	WGS84	BD47686	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47686_Pvir_Dia2013_COI	ESAV-175	1	BD47686_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47686	Sample	BD47686	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-175	COI	45	BD47686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-175	CTTAGTGGCATTAATTCGDAATCC	138.48336
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47693_Hsan_Wil2021_COI	GB78	1	BD47693_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Hsan_Wil2021_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	CCTGGGCCGGTATAGTAGGT	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47693_Mros_Gib2022_COI	GB78	1	BD47693_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Mros_Gib2022_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47693_Msal_Bot2012_COI	GB78	1	BD47693_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Msal_Bot2012_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	GYTAGTTCCRATGATGTTAGCTG	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47693_Pvir_Dia2013_COI	GB78	1	BD47693_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Pvir_Dia2013_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	CTTAGTGGCATTAATTCGDAATCC	151.23999
BD47693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.81635	WGS84	BD47693	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47693_Rhar_Sim2018_COI	GB78	1	BD47693_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47693	Sample	BD47693	targeted	Absent	Sample	Rhar_Sim2018_COI	GB78	COI	45	BD47693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB78	CTGCTGCTATTGCTCATG	151.23999
BD47697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86348	WGS84	BD47697	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47697_Hsan_Wil2021_COI	ESAV-123	1	BD47697_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47697	Sample	BD47697	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-123	COI	45	BD47697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-123	CCTGGGCCGGTATAGTAGGT	138.46865
BD47697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86348	WGS84	BD47697	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47697_Msal_Bot2012_COI	ESAV-123	1	BD47697_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47697	Sample	BD47697	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-123	COI	45	BD47697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-123	GYTAGTTCCRATGATGTTAGCTG	138.46865
BD47697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86348	WGS84	BD47697	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47697_Pvir_Dia2013_COI	ESAV-123	1	BD47697_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47697	Sample	BD47697	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-123	COI	45	BD47697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-123	CTTAGTGGCATTAATTCGDAATCC	138.46865
BD47701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84218	WGS84	BD47701	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47701_Hsan_Wil2021_COI	ESAV-049	1	BD47701_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47701	Sample	BD47701	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-049	COI	45	BD47701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-049	CCTGGGCCGGTATAGTAGGT	138.4638
BD47701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84218	WGS84	BD47701	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47701_Mros_Gib2022_COI	ESAV-049	1	BD47701_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47701	Sample	BD47701	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-049	COI	45	BD47701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-049	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4638
BD47701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84218	WGS84	BD47701	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47701_Msal_Bot2012_COI	ESAV-049	1	BD47701_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47701	Sample	BD47701	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-049	COI	45	BD47701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-049	GYTAGTTCCRATGATGTTAGCTG	138.4638
BD47701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84218	WGS84	BD47701	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47701_Pvir_Dia2013_COI	ESAV-049	1	BD47701_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47701	Sample	BD47701	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-049	COI	45	BD47701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-049	CTTAGTGGCATTAATTCGDAATCC	138.4638
BD47708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.78913	WGS84	BD47708	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47708_Asen_Wil2025_COI	GC91	1	BD47708_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47708	Sample	BD47708	targeted	Absent	Sample	Asen_Wil2025_COI	GC91	COI	45	BD47708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC91	GTTGTTGTTACAACACATGCTCTTTTG	151.17637
BD47711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82291	WGS84	BD47711	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47711_Asen_Wil2025_COI	GC41	1	BD47711_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47711	Sample	BD47711	targeted	Absent	Sample	Asen_Wil2025_COI	GC41	COI	45	BD47711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC41	GTTGTTGTTACAACACATGCTCTTTTG	151.24344
BD47712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82094	WGS84	BD47712	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47712_Rhar_Sim2018_COI	GD16	1	BD47712_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47712	Sample	BD47712	targeted	Absent	Sample	Rhar_Sim2018_COI	GD16	COI	45	BD47712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD16	CTGCTGCTATTGCTCATG	151.24059
BD47715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84269	WGS84	BD47715	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47715_Asen_Wil2025_COI	ESAV-140	1	BD47715_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47715	Sample	BD47715	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-140	COI	45	BD47715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-140	GTTGTTGTTACAACACATGCTCTTTTG	138.47128
BD47716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.86952	WGS84	BD47716	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47716_Msal_Bot2012_COI	ESAV-115	1	BD47716_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47716	Sample	BD47716	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-115	COI	45	BD47716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-115	GYTAGTTCCRATGATGTTAGCTG	138.46521
BD47719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83945	WGS84	BD47719	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47719_Asen_Wil2025_COI	ESAV-052	1	BD47719_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47719	Sample	BD47719	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-052	COI	45	BD47719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-052	GTTGTTGTTACAACACATGCTCTTTTG	138.46355
BD47722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79309	WGS84	BD47722	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47722_Asen_Wil2025_COI	GC97	1	BD47722_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47722	Sample	BD47722	targeted	Absent	Sample	Asen_Wil2025_COI	GC97	COI	45	BD47722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC97	GTTGTTGTTACAACACATGCTCTTTTG	151.18256
BD47723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82225	WGS84	BD47723	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47723_Rhar_Sim2018_COI	GC43	1	BD47723_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47723	Sample	BD47723	targeted	Absent	Sample	Rhar_Sim2018_COI	GC43	COI	45	BD47723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC43	CTGCTGCTATTGCTCATG	151.24316
BD47726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82309	WGS84	BD47726	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47726_Asen_Wil2025_COI	ESAV-070	1	BD47726_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47726	Sample	BD47726	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-070	COI	45	BD47726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-070	GTTGTTGTTACAACACATGCTCTTTTG	138.4627
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47727_Hsan_Wil2021_COI	GB25	1	BD47727_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Hsan_Wil2021_COI	GB25	COI	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	CCTGGGCCGGTATAGTAGGT	151.26186
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47727_Msal_Bot2012_COI	GB25	1	BD47727_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Msal_Bot2012_COI	GB25	COI	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	GYTAGTTCCRATGATGTTAGCTG	151.26186
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47727_Pvir_Dia2013_COI	GB25	1	BD47727_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Pvir_Dia2013_COI	GB25	COI	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	CTTAGTGGCATTAATTCGDAATCC	151.26186
BD47727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83199	WGS84	BD47727	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47727_Rhar_Sim2018_COI	GB25	1	BD47727_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47727	Sample	BD47727	targeted	Absent	Sample	Rhar_Sim2018_COI	GB25	COI	45	BD47727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB25	CTGCTGCTATTGCTCATG	151.26186
BD47730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83499	WGS84	BD47730	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47730_Rhar_Sim2018_COI	ESAV-057	1	BD47730_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47730	Sample	BD47730	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-057	COI	45	BD47730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-057	CTGCTGCTATTGCTCATG	138.46238
BD47733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.78577	WGS84	BD47733	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47733_Asen_Wil2025_COI	GA59	1	BD47733_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47733	Sample	BD47733	targeted	Absent	Sample	Asen_Wil2025_COI	GA59	COI	45	BD47733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA59	GTTGTTGTTACAACACATGCTCTTTTG	151.17282
BD47734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82643	WGS84	BD47734	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47734_Msal_Bot2012_COI	ESAV-152	1	BD47734_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47734	Sample	BD47734	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-152	COI	45	BD47734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-152	GYTAGTTCCRATGATGTTAGCTG	138.47756
BD47734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82643	WGS84	BD47734	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47734_Rhar_Sim2018_COI	ESAV-152	1	BD47734_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47734	Sample	BD47734	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-152	COI	45	BD47734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-152	CTGCTGCTATTGCTCATG	138.47756
BD47736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.85575	WGS84	BD47736	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47736_Vgib_Wil2025_COI	ESAV-130	1	BD47736_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47736	Sample	BD47736	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-130	COI	45	BD47736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-130	CCCCTCTTTACAAGTTCTTTAGGGT	138.47129
BD47737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81579	WGS84	BD47737	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47737_Asen_Wil2025_COI	ESAV-080	1	BD47737_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47737	Sample	BD47737	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-080	COI	45	BD47737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-080	GTTGTTGTTACAACACATGCTCTTTTG	138.46367
BD47738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.849	WGS84	BD47738	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47738_Msal_Bot2012_COI	ESAV-041	1	BD47738_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47738	Sample	BD47738	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-041	COI	45	BD47738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-041	GYTAGTTCCRATGATGTTAGCTG	138.4635
BD47738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.849	WGS84	BD47738	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47738_Rhar_Sim2018_COI	ESAV-041	1	BD47738_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47738	Sample	BD47738	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-041	COI	45	BD47738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-041	CTGCTGCTATTGCTCATG	138.4635
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47740_Asen_Wil2025_COI	GB65	1	BD47740_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Asen_Wil2025_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	GTTGTTGTTACAACACATGCTCTTTTG	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47740_Cjap_Sim2018_COI	GB65	1	BD47740_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Cjap_Sim2018_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47740_Esin_And2018_Cyb	GB65	1	BD47740_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Esin_And2018_Cyb	GB65	Cytb	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	ACCCCTCCTCATATCCAACCA	151.2453
BD47745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.79794	WGS84	BD47745	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47745_Mros_Gib2022_COI	ESAV-182	1	BD47745_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47745	Sample	BD47745	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-182	COI	45	BD47745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-182	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47958
BD47745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.79794	WGS84	BD47745	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47745_Pvir_Dia2013_COI	ESAV-182	1	BD47745_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47745	Sample	BD47745	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-182	COI	45	BD47745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-182	CTTAGTGGCATTAATTCGDAATCC	138.47958
BD47749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79231	WGS84	BD47749	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47749_Mros_Gib2022_COI	GC70	1	BD47749_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47749	Sample	BD47749	targeted	Absent	Sample	Mros_Gib2022_COI	GC70	COI	45	BD47749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC70	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21133
BD47749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79231	WGS84	BD47749	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47749_Msal_Bot2012_COI	GC70	1	BD47749_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47749	Sample	BD47749	targeted	Absent	Sample	Msal_Bot2012_COI	GC70	COI	45	BD47749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC70	GYTAGTTCCRATGATGTTAGCTG	151.21133
BD47749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79231	WGS84	BD47749	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47749_Pvir_Dia2013_COI	GC70	1	BD47749_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47749	Sample	BD47749	targeted	Absent	Sample	Pvir_Dia2013_COI	GC70	COI	45	BD47749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC70	CTTAGTGGCATTAATTCGDAATCC	151.21133
BD47751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82647	WGS84	BD47751	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47751_Asen_Wil2025_COI	GB30	1	BD47751_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47751	Sample	BD47751	targeted	Absent	Sample	Asen_Wil2025_COI	GB30	COI	45	BD47751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB30	GTTGTTGTTACAACACATGCTCTTTTG	151.24714
BD47752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.7873	WGS84	BD47752	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47752_Mros_Gib2022_COI	GC86	1	BD47752_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47752	Sample	BD47752	targeted	Absent	Sample	Mros_Gib2022_COI	GC86	COI	45	BD47752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC86	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17401
BD47752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.7873	WGS84	BD47752	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47752_Pvir_Dia2013_COI	GC86	1	BD47752_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47752	Sample	BD47752	targeted	Absent	Sample	Pvir_Dia2013_COI	GC86	COI	45	BD47752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC86	CTTAGTGGCATTAATTCGDAATCC	151.17401
BD47756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80662	WGS84	BD47756	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47756_Mros_Gib2022_COI	ESAV-088	1	BD47756_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47756	Sample	BD47756	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-088	COI	45	BD47756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-088	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46468
BD47756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80662	WGS84	BD47756	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47756_Pvir_Dia2013_COI	ESAV-088	1	BD47756_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47756	Sample	BD47756	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-088	COI	45	BD47756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-088	CTTAGTGGCATTAATTCGDAATCC	138.46468
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47763_Mros_Gib2022_COI	GB11	1	BD47763_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Mros_Gib2022_COI	GB11	COI	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31153
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47763_Msal_Bot2012_COI	GB11	1	BD47763_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Msal_Bot2012_COI	GB11	COI	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	GYTAGTTCCRATGATGTTAGCTG	151.31153
BD47763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85079	WGS84	BD47763	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47763_Pvir_Dia2013_COI	GB11	1	BD47763_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47763	Sample	BD47763	targeted	Absent	Sample	Pvir_Dia2013_COI	GB11	COI	45	BD47763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB11	CTTAGTGGCATTAATTCGDAATCC	151.31153
BD47767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84898	WGS84	BD47767	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47767_Mros_Gib2022_COI	ESAV-135	1	BD47767_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47767	Sample	BD47767	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-135	COI	45	BD47767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-135	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47102
BD47767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84898	WGS84	BD47767	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47767_Pvir_Dia2013_COI	ESAV-135	1	BD47767_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47767	Sample	BD47767	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-135	COI	45	BD47767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-135	CTTAGTGGCATTAATTCGDAATCC	138.47102
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47770_Mros_Gib2022_COI	ESAV-042	1	BD47770_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-042	COI	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46339
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47770_Msal_Bot2012_COI	ESAV-042	1	BD47770_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-042	COI	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	GYTAGTTCCRATGATGTTAGCTG	138.46339
BD47770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.8485	WGS84	BD47770	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47770_Pvir_Dia2013_COI	ESAV-042	1	BD47770_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47770	Sample	BD47770	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-042	COI	45	BD47770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-042	CTTAGTGGCATTAATTCGDAATCC	138.46339
BD47773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82461	WGS84	BD47773	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47773_Cjap_Sim2018_COI	GB32	1	BD47773_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47773	Sample	BD47773	targeted	Absent	Sample	Cjap_Sim2018_COI	GB32	COI	45	BD47773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB32	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.24755
BD47773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82461	WGS84	BD47773	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47773_Esin_And2018_Cyb	GB32	1	BD47773_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47773	Sample	BD47773	targeted	Absent	Sample	Esin_And2018_Cyb	GB32	Cytb	45	BD47773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB32	ACCCCTCCTCATATCCAACCA	151.24755
BD47774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.95981	WGS84	BD47774	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47774_Mros_Gib2022_COI	KPT-A37	1	BD47774_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47774	Sample	BD47774	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A37	COI	45	BD47774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A37	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77697
BD47774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.95981	WGS84	BD47774	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47774_Pvir_Dia2013_COI	KPT-A37	1	BD47774_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47774	Sample	BD47774	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A37	COI	45	BD47774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A37	CTTAGTGGCATTAATTCGDAATCC	137.77697
BD47778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80752	WGS84	BD47778	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47778_Mros_Gib2022_COI	ESAV-087	1	BD47778_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47778	Sample	BD47778	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-087	COI	45	BD47778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-087	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46454
BD47778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80752	WGS84	BD47778	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47778_Pvir_Dia2013_COI	ESAV-087	1	BD47778_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47778	Sample	BD47778	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-087	COI	45	BD47778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-087	CTTAGTGGCATTAATTCGDAATCC	138.46454
BD47780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79329	WGS84	BD47780	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47780_Esin_And2018_Cyb	GB59	1	BD47780_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47780	Sample	BD47780	targeted	Absent	Sample	Esin_And2018_Cyb	GB59	Cytb	45	BD47780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB59	ACCCCTCCTCATATCCAACCA	151.18479
BD47780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79329	WGS84	BD47780	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47780_Mjap_Gib2022_COI	GB59	1	BD47780_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47780	Sample	BD47780	targeted	Absent	Sample	Mjap_Gib2022_COI	GB59	COI	45	BD47780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB59	AATTTAATTGTGACTAGGCATGGTCTG	151.18479
BD47781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85344	WGS84	BD47781	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47781_Mros_Gib2022_COI	GA28	1	BD47781_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47781	Sample	BD47781	targeted	Absent	Sample	Mros_Gib2022_COI	GA28	COI	45	BD47781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA28	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31818
BD47781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85344	WGS84	BD47781	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47781_Pvir_Dia2013_COI	GA28	1	BD47781_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47781	Sample	BD47781	targeted	Absent	Sample	Pvir_Dia2013_COI	GA28	COI	45	BD47781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA28	CTTAGTGGCATTAATTCGDAATCC	151.31818
BD47784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80632	WGS84	BD47784	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47784_Cjap_Sim2018_COI	GB39	1	BD47784_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47784	Sample	BD47784	targeted	Absent	Sample	Cjap_Sim2018_COI	GB39	COI	45	BD47784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB39	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.21532
BD47784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80632	WGS84	BD47784	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47784_Esin_And2018_Cyb	GB39	1	BD47784_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47784	Sample	BD47784	targeted	Absent	Sample	Esin_And2018_Cyb	GB39	Cytb	45	BD47784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB39	ACCCCTCCTCATATCCAACCA	151.21532
BD47788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86112	WGS84	BD47788	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47788_Cjap_Sim2018_COI	ESAV-105	1	BD47788_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47788	Sample	BD47788	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-105	COI	45	BD47788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-105	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46483
BD47791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84765	WGS84	BD47791	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47791_Cjap_Sim2018_COI	ESAV-043	1	BD47791_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47791	Sample	BD47791	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-043	COI	45	BD47791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-043	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46365
BD47794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82305	WGS84	BD47794	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47794_Pper_Dia2013_COI	GB72	1	BD47794_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47794	Sample	BD47794	targeted	Absent	Sample	Pper_Dia2013_COI	GB72	COI	45	BD47794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB72	CTTAGTGGCATTAATTCGDAATCC	151.24253
BD47794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82305	WGS84	BD47794	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47794_Vgib_Wil2025_COI	GB72	1	BD47794_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47794	Sample	BD47794	targeted	Absent	Sample	Vgib_Wil2025_COI	GB72	COI	45	BD47794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB72	CCCCTCTTTACAAGTTCTTTAGGGT	151.24253
BD47795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.95804	WGS84	BD47795	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47795_Cjap_Sim2018_COI	ESAV-240	1	BD47795_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47795	Sample	BD47795	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-240	COI	45	BD47795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-240	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77699
BD47799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.83699	WGS84	BD47799	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47799_Cjap_Sim2018_COI	ESAV-142	1	BD47799_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47799	Sample	BD47799	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-142	COI	45	BD47799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-142	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47309
BD47803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79104	WGS84	BD47803	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47803_Cjap_Sim2018_COI	GC69	1	BD47803_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47803	Sample	BD47803	targeted	Absent	Sample	Cjap_Sim2018_COI	GC69	COI	45	BD47803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC69	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.21074
BD47804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.96279	WGS84	BD47804	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47804_Pvir_Dia2013_COI	ESAV-237	1	BD47804_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47804	Sample	BD47804	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-237	COI	45	BD47804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-237	CTTAGTGGCATTAATTCGDAATCC	137.77656
BD47807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86837	WGS84	BD47807	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47807_Cjap_Sim2018_COI	ESAV-117	1	BD47807_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47807	Sample	BD47807	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-117	COI	45	BD47807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-117	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46575
BD47808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.78842	WGS84	BD47808	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47808_Pvir_Dia2013_COI	GC94	1	BD47808_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47808	Sample	BD47808	targeted	Absent	Sample	Pvir_Dia2013_COI	GC94	COI	45	BD47808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC94	CTTAGTGGCATTAATTCGDAATCC	151.17828
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47810_Asen_Wil2025_COI	GB41	1	BD47810_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Asen_Wil2025_COI	GB41	COI	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	GTTGTTGTTACAACACATGCTCTTTTG	151.2175
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47810_Cjap_Sim2018_COI	GB41	1	BD47810_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Cjap_Sim2018_COI	GB41	COI	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2175
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47810_Mjap_Gib2022_COI	GB41	1	BD47810_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Mjap_Gib2022_COI	GB41	COI	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	AATTTAATTGTGACTAGGCATGGTCTG	151.2175
BD47814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8156	WGS84	BD47814	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47814_Cjap_Sim2018_COI	GB81	1	BD47814_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47814	Sample	BD47814	targeted	Absent	Sample	Cjap_Sim2018_COI	GB81	COI	45	BD47814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB81	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.23658
BD47815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.85421	WGS84	BD47815	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47815_Pvir_Dia2013_COI	GA23	1	BD47815_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47815	Sample	BD47815	targeted	Absent	Sample	Pvir_Dia2013_COI	GA23	COI	45	BD47815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA23	CTTAGTGGCATTAATTCGDAATCC	151.32248
BD47817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.95886	WGS84	BD47817	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47817_Vgib_Wil2025_COI	KPT-A39	1	BD47817_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47817	Sample	BD47817	targeted	Absent	Sample	Vgib_Wil2025_COI	KPT-A39	COI	45	BD47817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A39	CCCCTCTTTACAAGTTCTTTAGGGT	137.77591
BD47818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.79976	WGS84	BD47818	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47818_Cjap_Sim2018_COI	ESAV-184	1	BD47818_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47818	Sample	BD47818	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-184	COI	45	BD47818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-184	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47774
BD47821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.82491	WGS84	BD47821	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47821_Cjap_Sim2018_COI	ESAV-068	1	BD47821_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47821	Sample	BD47821	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-068	COI	45	BD47821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-068	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46246
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11307_Cmae_Bot2011_COI	CNSA-001	1	BE11307_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	ATGAACAGTCTATCCTCCTTTAG	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11307_Hsan_Wil2021_COI	CNSA-001	1	BE11307_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	CCTGGGCCGGTATAGTAGGT	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11307_Mros_Gib2022_COI	CNSA-001	1	BE11307_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11307_Msal_Bot2012_COI	CNSA-001	1	BE11307_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	GYTAGTTCCRATGATGTTAGCTG	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11307_Mstr_Wil2021_COI	CNSA-001	1	BE11307_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	GTAGTGGTAACTACTCATGCTTTA	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11307_Pvir_Dia2013_COI	CNSA-001	1	BE11307_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	CTTAGTGGCATTAATTCGDAATCC	145.77385
BE11307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.95032	WGS84	BE11307	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11307_Rhar_Sim2018_COI	CNSA-001	1	BE11307_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11307	Sample	BE11307	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-001	COI	45	BE11307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-001	CTGCTGCTATTGCTCATG	145.77385
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11309_Aamu_Bax2006_COI	CNSA-003	1	BE11309_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	GCACAACCGGGATCTTTACTTC	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11309_Pcan_Bot2011_IGS	CNSA-003	1	BE11309_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-003	IGS	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	CGTAATCCTCAGTACTGGCTA	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11309_Pper_Dia2013_COI	CNSA-003	1	BE11309_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	CTTAGTGGCATTAATTCGDAATCC	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11309_Sspa_Oph2007_COI	CNSA-003	1	BE11309_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	GAAGGCCTTCCGATGATC	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11309_Upin_Bot2011_COI	CNSA-003	1	BE11309_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	CTTTAATTACAGCGTTTTTATTGTTGT	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11309_Vgib_Wil2025_COI	CNSA-003	1	BE11309_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	CCCCTCTTTACAAGTTCTTTAGGGT	145.77464
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11310_Cmae_Bot2011_COI	CNSA-004	1	BE11310_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	ATGAACAGTCTATCCTCCTTTAG	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11310_Hsan_Wil2021_COI	CNSA-004	1	BE11310_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	CCTGGGCCGGTATAGTAGGT	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11310_Mros_Gib2022_COI	CNSA-004	1	BE11310_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11310_Msal_Bot2012_COI	CNSA-004	1	BE11310_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	GYTAGTTCCRATGATGTTAGCTG	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11310_Mstr_Wil2021_COI	CNSA-004	1	BE11310_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	GTAGTGGTAACTACTCATGCTTTA	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11310_Pvir_Dia2013_COI	CNSA-004	1	BE11310_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	CTTAGTGGCATTAATTCGDAATCC	145.77622
BE11310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94254	WGS84	BE11310	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11310_Rhar_Sim2018_COI	CNSA-004	1	BE11310_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11310	Sample	BE11310	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-004	COI	45	BE11310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-004	CTGCTGCTATTGCTCATG	145.77622
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11312_Aamu_Bax2006_COI	CNSA-006	1	BE11312_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	GCACAACCGGGATCTTTACTTC	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11312_Pcan_Bot2011_IGS	CNSA-006	1	BE11312_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-006	IGS	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	CGTAATCCTCAGTACTGGCTA	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11312_Pper_Dia2013_COI	CNSA-006	1	BE11312_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	CTTAGTGGCATTAATTCGDAATCC	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11312_Sspa_Oph2007_COI	CNSA-006	1	BE11312_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	GAAGGCCTTCCGATGATC	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11312_Upin_Bot2011_COI	CNSA-006	1	BE11312_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	CTTTAATTACAGCGTTTTTATTGTTGT	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11312_Vgib_Wil2025_COI	CNSA-006	1	BE11312_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	CCCCTCTTTACAAGTTCTTTAGGGT	145.78256
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11313_Asen_Wil2025_COI	CNSA-007	1	BE11313_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	GTTGTTGTTACAACACATGCTCTTTTG	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11313_Cjap_Sim2018_COI	CNSA-007	1	BE11313_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11313_Esin_And2018_Cyb	CNSA-007	1	BE11313_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-007	Cytb	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	ACCCCTCCTCATATCCAACCA	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11313_Mgig_Bot2012_COI	CNSA-007	1	BE11313_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	TCTTATTCGTTGGAGACTTTATAACCCT	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11313_Mjap_Gib2022_COI	CNSA-007	1	BE11313_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	AATTTAATTGTGACTAGGCATGGTCTG	145.7816
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11314_Cmae_Bot2011_COI	CNSA-008	1	BE11314_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	ATGAACAGTCTATCCTCCTTTAG	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11314_Hsan_Wil2021_COI	CNSA-008	1	BE11314_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	CCTGGGCCGGTATAGTAGGT	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11314_Mros_Gib2022_COI	CNSA-008	1	BE11314_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11314_Msal_Bot2012_COI	CNSA-008	1	BE11314_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	GYTAGTTCCRATGATGTTAGCTG	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11314_Mstr_Wil2021_COI	CNSA-008	1	BE11314_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	GTAGTGGTAACTACTCATGCTTTA	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11314_Pvir_Dia2013_COI	CNSA-008	1	BE11314_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	CTTAGTGGCATTAATTCGDAATCC	145.78272
BE11314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94033	WGS84	BE11314	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11314_Rhar_Sim2018_COI	CNSA-008	1	BE11314_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11314	Sample	BE11314	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-008	COI	45	BE11314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-008	CTGCTGCTATTGCTCATG	145.78272
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11316_Aamu_Bax2006_COI	CNSA-010	1	BE11316_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	GCACAACCGGGATCTTTACTTC	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11316_Pcan_Bot2011_IGS	CNSA-010	1	BE11316_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-010	IGS	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	CGTAATCCTCAGTACTGGCTA	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11316_Pper_Dia2013_COI	CNSA-010	1	BE11316_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	CTTAGTGGCATTAATTCGDAATCC	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11316_Sspa_Oph2007_COI	CNSA-010	1	BE11316_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	GAAGGCCTTCCGATGATC	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11316_Upin_Bot2011_COI	CNSA-010	1	BE11316_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	CTTTAATTACAGCGTTTTTATTGTTGT	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11316_Vgib_Wil2025_COI	CNSA-010	1	BE11316_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	CCCCTCTTTACAAGTTCTTTAGGGT	145.78192
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11317_Asen_Wil2025_COI	CNSA-011	1	BE11317_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	GTTGTTGTTACAACACATGCTCTTTTG	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11317_Cjap_Sim2018_COI	CNSA-011	1	BE11317_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11317_Esin_And2018_Cyb	CNSA-011	1	BE11317_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-011	Cytb	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	ACCCCTCCTCATATCCAACCA	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11317_Mgig_Bot2012_COI	CNSA-011	1	BE11317_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	TCTTATTCGTTGGAGACTTTATAACCCT	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11317_Mjap_Gib2022_COI	CNSA-011	1	BE11317_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	AATTTAATTGTGACTAGGCATGGTCTG	145.78089
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11318_Cmae_Bot2011_COI	CNSA-012	1	BE11318_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	ATGAACAGTCTATCCTCCTTTAG	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11318_Hsan_Wil2021_COI	CNSA-012	1	BE11318_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	CCTGGGCCGGTATAGTAGGT	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11318_Mros_Gib2022_COI	CNSA-012	1	BE11318_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11318_Msal_Bot2012_COI	CNSA-012	1	BE11318_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	GYTAGTTCCRATGATGTTAGCTG	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11318_Mstr_Wil2021_COI	CNSA-012	1	BE11318_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	GTAGTGGTAACTACTCATGCTTTA	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11318_Pvir_Dia2013_COI	CNSA-012	1	BE11318_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	CTTAGTGGCATTAATTCGDAATCC	145.78149
BE11318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93613	WGS84	BE11318	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11318_Rhar_Sim2018_COI	CNSA-012	1	BE11318_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11318	Sample	BE11318	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-012	COI	45	BE11318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-012	CTGCTGCTATTGCTCATG	145.78149
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11320_Asen_Wil2025_COI	CNSA-014	1	BE11320_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	GTTGTTGTTACAACACATGCTCTTTTG	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11320_Cjap_Sim2018_COI	CNSA-014	1	BE11320_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11320_Esin_And2018_Cyb	CNSA-014	1	BE11320_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-014	Cytb	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	ACCCCTCCTCATATCCAACCA	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11320_Mgig_Bot2012_COI	CNSA-014	1	BE11320_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	TCTTATTCGTTGGAGACTTTATAACCCT	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11320_Mjap_Gib2022_COI	CNSA-014	1	BE11320_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	AATTTAATTGTGACTAGGCATGGTCTG	145.78207
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11321_Cmae_Bot2011_COI	CNSA-015	1	BE11321_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	ATGAACAGTCTATCCTCCTTTAG	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11321_Hsan_Wil2021_COI	CNSA-015	1	BE11321_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	CCTGGGCCGGTATAGTAGGT	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11321_Mros_Gib2022_COI	CNSA-015	1	BE11321_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11321_Msal_Bot2012_COI	CNSA-015	1	BE11321_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	GYTAGTTCCRATGATGTTAGCTG	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11321_Mstr_Wil2021_COI	CNSA-015	1	BE11321_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	GTAGTGGTAACTACTCATGCTTTA	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11321_Pvir_Dia2013_COI	CNSA-015	1	BE11321_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	CTTAGTGGCATTAATTCGDAATCC	145.78151
BE11321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93324	WGS84	BE11321	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11321_Rhar_Sim2018_COI	CNSA-015	1	BE11321_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11321	Sample	BE11321	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-015	COI	45	BE11321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-015	CTGCTGCTATTGCTCATG	145.78151
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11323_Aamu_Bax2006_COI	CNSA-017	1	BE11323_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	GCACAACCGGGATCTTTACTTC	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11323_Pcan_Bot2011_IGS	CNSA-017	1	BE11323_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-017	IGS	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	CGTAATCCTCAGTACTGGCTA	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11323_Sspa_Oph2007_COI	CNSA-017	1	BE11323_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	GAAGGCCTTCCGATGATC	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11323_Upin_Bot2011_COI	CNSA-017	1	BE11323_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	CTTTAATTACAGCGTTTTTATTGTTGT	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11323_Vgib_Wil2025_COI	CNSA-017	1	BE11323_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	CCCCTCTTTACAAGTTCTTTAGGGT	145.78215
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11324_Asen_Wil2025_COI	CNSA-018	1	BE11324_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	GTTGTTGTTACAACACATGCTCTTTTG	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11324_Cjap_Sim2018_COI	CNSA-018	1	BE11324_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11324_Esin_And2018_Cyb	CNSA-018	1	BE11324_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-018	Cytb	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	ACCCCTCCTCATATCCAACCA	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11324_Mgig_Bot2012_COI	CNSA-018	1	BE11324_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	TCTTATTCGTTGGAGACTTTATAACCCT	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11324_Mjap_Gib2022_COI	CNSA-018	1	BE11324_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	AATTTAATTGTGACTAGGCATGGTCTG	145.78129
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11325_Cmae_Bot2011_COI	CNSA-019	1	BE11325_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	ATGAACAGTCTATCCTCCTTTAG	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11325_Hsan_Wil2021_COI	CNSA-019	1	BE11325_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	CCTGGGCCGGTATAGTAGGT	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11325_Mros_Gib2022_COI	CNSA-019	1	BE11325_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11325_Msal_Bot2012_COI	CNSA-019	1	BE11325_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	GYTAGTTCCRATGATGTTAGCTG	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11325_Mstr_Wil2021_COI	CNSA-019	1	BE11325_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	GTAGTGGTAACTACTCATGCTTTA	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11325_Pvir_Dia2013_COI	CNSA-019	1	BE11325_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	CTTAGTGGCATTAATTCGDAATCC	145.78272
BE11325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93025	WGS84	BE11325	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11325_Rhar_Sim2018_COI	CNSA-019	1	BE11325_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11325	Sample	BE11325	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-019	COI	45	BE11325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-019	CTGCTGCTATTGCTCATG	145.78272
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11327_Aamu_Bax2006_COI	CNSA-021	1	BE11327_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	GCACAACCGGGATCTTTACTTC	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11327_Pcan_Bot2011_IGS	CNSA-021	1	BE11327_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-021	IGS	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	CGTAATCCTCAGTACTGGCTA	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11327_Pper_Dia2013_COI	CNSA-021	1	BE11327_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	CTTAGTGGCATTAATTCGDAATCC	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11327_Sspa_Oph2007_COI	CNSA-021	1	BE11327_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	GAAGGCCTTCCGATGATC	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11327_Upin_Bot2011_COI	CNSA-021	1	BE11327_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	CTTTAATTACAGCGTTTTTATTGTTGT	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11327_Vgib_Wil2025_COI	CNSA-021	1	BE11327_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	CCCCTCTTTACAAGTTCTTTAGGGT	145.7835
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11328_Asen_Wil2025_COI	CNSA-022	1	BE11328_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	GTTGTTGTTACAACACATGCTCTTTTG	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11328_Cjap_Sim2018_COI	CNSA-022	1	BE11328_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11328_Esin_And2018_Cyb	CNSA-022	1	BE11328_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-022	Cytb	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	ACCCCTCCTCATATCCAACCA	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11328_Mgig_Bot2012_COI	CNSA-022	1	BE11328_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	TCTTATTCGTTGGAGACTTTATAACCCT	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11328_Mjap_Gib2022_COI	CNSA-022	1	BE11328_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	AATTTAATTGTGACTAGGCATGGTCTG	145.78172
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11329_Cmae_Bot2011_COI	CNSA-023	1	BE11329_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	ATGAACAGTCTATCCTCCTTTAG	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11329_Hsan_Wil2021_COI	CNSA-023	1	BE11329_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	CCTGGGCCGGTATAGTAGGT	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11329_Mros_Gib2022_COI	CNSA-023	1	BE11329_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11329_Msal_Bot2012_COI	CNSA-023	1	BE11329_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	GYTAGTTCCRATGATGTTAGCTG	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11329_Mstr_Wil2021_COI	CNSA-023	1	BE11329_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	GTAGTGGTAACTACTCATGCTTTA	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11329_Pvir_Dia2013_COI	CNSA-023	1	BE11329_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	CTTAGTGGCATTAATTCGDAATCC	145.78342
BE11329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92592	WGS84	BE11329	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11329_Rhar_Sim2018_COI	CNSA-023	1	BE11329_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11329	Sample	BE11329	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-023	COI	45	BE11329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-023	CTGCTGCTATTGCTCATG	145.78342
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11330_Aamu_Bax2006_COI	CNSA-024	1	BE11330_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	GCACAACCGGGATCTTTACTTC	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11330_Pcan_Bot2011_IGS	CNSA-024	1	BE11330_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-024	IGS	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	CGTAATCCTCAGTACTGGCTA	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11330_Pper_Dia2013_COI	CNSA-024	1	BE11330_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	CTTAGTGGCATTAATTCGDAATCC	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11330_Sspa_Oph2007_COI	CNSA-024	1	BE11330_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	GAAGGCCTTCCGATGATC	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11330_Upin_Bot2011_COI	CNSA-024	1	BE11330_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	CTTTAATTACAGCGTTTTTATTGTTGT	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11330_Vgib_Wil2025_COI	CNSA-024	1	BE11330_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	CCCCTCTTTACAAGTTCTTTAGGGT	145.78272
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11331_Asen_Wil2025_COI	CNSA-025	1	BE11331_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	GTTGTTGTTACAACACATGCTCTTTTG	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11331_Cjap_Sim2018_COI	CNSA-025	1	BE11331_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11331_Esin_And2018_Cyb	CNSA-025	1	BE11331_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-025	Cytb	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	ACCCCTCCTCATATCCAACCA	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11331_Mgig_Bot2012_COI	CNSA-025	1	BE11331_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	TCTTATTCGTTGGAGACTTTATAACCCT	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11331_Mjap_Gib2022_COI	CNSA-025	1	BE11331_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	AATTTAATTGTGACTAGGCATGGTCTG	145.78397
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11332_Cmae_Bot2011_COI	CNSA-026	1	BE11332_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	ATGAACAGTCTATCCTCCTTTAG	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11332_Hsan_Wil2021_COI	CNSA-026	1	BE11332_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	CCTGGGCCGGTATAGTAGGT	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11332_Mros_Gib2022_COI	CNSA-026	1	BE11332_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11332_Msal_Bot2012_COI	CNSA-026	1	BE11332_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	GYTAGTTCCRATGATGTTAGCTG	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11332_Mstr_Wil2021_COI	CNSA-026	1	BE11332_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	GTAGTGGTAACTACTCATGCTTTA	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11332_Pvir_Dia2013_COI	CNSA-026	1	BE11332_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	CTTAGTGGCATTAATTCGDAATCC	145.78469
BE11332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92283	WGS84	BE11332	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11332_Rhar_Sim2018_COI	CNSA-026	1	BE11332_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11332	Sample	BE11332	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-026	COI	45	BE11332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-026	CTGCTGCTATTGCTCATG	145.78469
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11334_Aamu_Bax2006_COI	CNSA-028	1	BE11334_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	GCACAACCGGGATCTTTACTTC	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11334_Pcan_Bot2011_IGS	CNSA-028	1	BE11334_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-028	IGS	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	CGTAATCCTCAGTACTGGCTA	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11334_Pper_Dia2013_COI	CNSA-028	1	BE11334_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	CTTAGTGGCATTAATTCGDAATCC	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11334_Sspa_Oph2007_COI	CNSA-028	1	BE11334_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	GAAGGCCTTCCGATGATC	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11334_Upin_Bot2011_COI	CNSA-028	1	BE11334_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	CTTTAATTACAGCGTTTTTATTGTTGT	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11334_Vgib_Wil2025_COI	CNSA-028	1	BE11334_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	CCCCTCTTTACAAGTTCTTTAGGGT	145.78504
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11335_Asen_Wil2025_COI	CNSA-029	1	BE11335_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	GTTGTTGTTACAACACATGCTCTTTTG	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11335_Cjap_Sim2018_COI	CNSA-029	1	BE11335_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11335_Esin_And2018_Cyb	CNSA-029	1	BE11335_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-029	Cytb	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	ACCCCTCCTCATATCCAACCA	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11335_Mgig_Bot2012_COI	CNSA-029	1	BE11335_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	TCTTATTCGTTGGAGACTTTATAACCCT	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11335_Mjap_Gib2022_COI	CNSA-029	1	BE11335_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	AATTTAATTGTGACTAGGCATGGTCTG	145.78451
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11336_Cmae_Bot2011_COI	CNSA-030	1	BE11336_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	ATGAACAGTCTATCCTCCTTTAG	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11336_Hsan_Wil2021_COI	CNSA-030	1	BE11336_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	CCTGGGCCGGTATAGTAGGT	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11336_Mros_Gib2022_COI	CNSA-030	1	BE11336_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11336_Msal_Bot2012_COI	CNSA-030	1	BE11336_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	GYTAGTTCCRATGATGTTAGCTG	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11336_Mstr_Wil2021_COI	CNSA-030	1	BE11336_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	GTAGTGGTAACTACTCATGCTTTA	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11336_Pvir_Dia2013_COI	CNSA-030	1	BE11336_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	CTTAGTGGCATTAATTCGDAATCC	145.78522
BE11336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9186	WGS84	BE11336	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11336_Rhar_Sim2018_COI	CNSA-030	1	BE11336_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11336	Sample	BE11336	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-030	COI	45	BE11336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-030	CTGCTGCTATTGCTCATG	145.78522
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11338_Aamu_Bax2006_COI	CNSA-032	1	BE11338_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	GCACAACCGGGATCTTTACTTC	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11338_Pcan_Bot2011_IGS	CNSA-032	1	BE11338_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-032	IGS	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	CGTAATCCTCAGTACTGGCTA	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11338_Pper_Dia2013_COI	CNSA-032	1	BE11338_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	CTTAGTGGCATTAATTCGDAATCC	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11338_Sspa_Oph2007_COI	CNSA-032	1	BE11338_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	GAAGGCCTTCCGATGATC	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11338_Upin_Bot2011_COI	CNSA-032	1	BE11338_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	CTTTAATTACAGCGTTTTTATTGTTGT	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11338_Vgib_Wil2025_COI	CNSA-032	1	BE11338_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	CCCCTCTTTACAAGTTCTTTAGGGT	145.78565
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11339_Asen_Wil2025_COI	CNSA-033	1	BE11339_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	GTTGTTGTTACAACACATGCTCTTTTG	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11339_Cjap_Sim2018_COI	CNSA-033	1	BE11339_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11339_Esin_And2018_Cyb	CNSA-033	1	BE11339_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-033	Cytb	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	ACCCCTCCTCATATCCAACCA	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11339_Mgig_Bot2012_COI	CNSA-033	1	BE11339_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	TCTTATTCGTTGGAGACTTTATAACCCT	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11339_Mjap_Gib2022_COI	CNSA-033	1	BE11339_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	AATTTAATTGTGACTAGGCATGGTCTG	145.782
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11341_Aamu_Bax2006_COI	CNSA-035	1	BE11341_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	GCACAACCGGGATCTTTACTTC	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11341_Pcan_Bot2011_IGS	CNSA-035	1	BE11341_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-035	IGS	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	CGTAATCCTCAGTACTGGCTA	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11341_Pper_Dia2013_COI	CNSA-035	1	BE11341_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	CTTAGTGGCATTAATTCGDAATCC	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11341_Sspa_Oph2007_COI	CNSA-035	1	BE11341_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	GAAGGCCTTCCGATGATC	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11341_Upin_Bot2011_COI	CNSA-035	1	BE11341_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	CTTTAATTACAGCGTTTTTATTGTTGT	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11341_Vgib_Wil2025_COI	CNSA-035	1	BE11341_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	CCCCTCTTTACAAGTTCTTTAGGGT	145.78241
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11346_Asen_Wil2025_COI	TSVA-001	1	BE11346_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	GTTGTTGTTACAACACATGCTCTTTTG	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11346_Cjap_Sim2018_COI	TSVA-001	1	BE11346_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11346_Esin_And2018_Cyb	TSVA-001	1	BE11346_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-001	Cytb	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	ACCCCTCCTCATATCCAACCA	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11346_Mgig_Bot2012_COI	TSVA-001	1	BE11346_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	TCTTATTCGTTGGAGACTTTATAACCCT	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11346_Mjap_Gib2022_COI	TSVA-001	1	BE11346_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	AATTTAATTGTGACTAGGCATGGTCTG	146.8425
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11347_Cmae_Bot2011_COI	TSVA-002	1	BE11347_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	ATGAACAGTCTATCCTCCTTTAG	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11347_Hsan_Wil2021_COI	TSVA-002	1	BE11347_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	CCTGGGCCGGTATAGTAGGT	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11347_Mros_Gib2022_COI	TSVA-002	1	BE11347_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11347_Msal_Bot2012_COI	TSVA-002	1	BE11347_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	GYTAGTTCCRATGATGTTAGCTG	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11347_Mstr_Wil2021_COI	TSVA-002	1	BE11347_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	GTAGTGGTAACTACTCATGCTTTA	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11347_Pvir_Dia2013_COI	TSVA-002	1	BE11347_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	CTTAGTGGCATTAATTCGDAATCC	146.84366
BE11347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24261	WGS84	BE11347	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11347_Rhar_Sim2018_COI	TSVA-002	1	BE11347_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11347	Sample	BE11347	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-002	COI	45	BE11347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-002	CTGCTGCTATTGCTCATG	146.84366
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11349_Aamu_Bax2006_COI	TSVA-004	1	BE11349_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	GCACAACCGGGATCTTTACTTC	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11349_Pcan_Bot2011_IGS	TSVA-004	1	BE11349_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-004	IGS	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	CGTAATCCTCAGTACTGGCTA	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11349_Pper_Dia2013_COI	TSVA-004	1	BE11349_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	CTTAGTGGCATTAATTCGDAATCC	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11349_Sspa_Oph2007_COI	TSVA-004	1	BE11349_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	GAAGGCCTTCCGATGATC	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11349_Upin_Bot2011_COI	TSVA-004	1	BE11349_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	CTTTAATTACAGCGTTTTTATTGTTGT	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11349_Vgib_Wil2025_COI	TSVA-004	1	BE11349_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	CCCCTCTTTACAAGTTCTTTAGGGT	146.84148
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11350_Cmae_Bot2011_COI	TSVA-005	1	BE11350_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	ATGAACAGTCTATCCTCCTTTAG	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11350_Hsan_Wil2021_COI	TSVA-005	1	BE11350_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	CCTGGGCCGGTATAGTAGGT	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11350_Mros_Gib2022_COI	TSVA-005	1	BE11350_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11350_Msal_Bot2012_COI	TSVA-005	1	BE11350_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	GYTAGTTCCRATGATGTTAGCTG	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11350_Mstr_Wil2021_COI	TSVA-005	1	BE11350_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	GTAGTGGTAACTACTCATGCTTTA	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11350_Pvir_Dia2013_COI	TSVA-005	1	BE11350_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	CTTAGTGGCATTAATTCGDAATCC	146.84022
BE11350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24231	WGS84	BE11350	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11350_Rhar_Sim2018_COI	TSVA-005	1	BE11350_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11350	Sample	BE11350	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-005	COI	45	BE11350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-005	CTGCTGCTATTGCTCATG	146.84022
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11352_Aamu_Bax2006_COI	TSVA-007	1	BE11352_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	GCACAACCGGGATCTTTACTTC	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11352_Pcan_Bot2011_IGS	TSVA-007	1	BE11352_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-007	IGS	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	CGTAATCCTCAGTACTGGCTA	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11352_Pper_Dia2013_COI	TSVA-007	1	BE11352_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	CTTAGTGGCATTAATTCGDAATCC	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11352_Sspa_Oph2007_COI	TSVA-007	1	BE11352_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	GAAGGCCTTCCGATGATC	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11352_Upin_Bot2011_COI	TSVA-007	1	BE11352_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	CTTTAATTACAGCGTTTTTATTGTTGT	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11352_Vgib_Wil2025_COI	TSVA-007	1	BE11352_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	CCCCTCTTTACAAGTTCTTTAGGGT	146.84007
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11353_Asen_Wil2025_COI	TSVA-008	1	BE11353_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	GTTGTTGTTACAACACATGCTCTTTTG	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11353_Cjap_Sim2018_COI	TSVA-008	1	BE11353_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11353_Esin_And2018_Cyb	TSVA-008	1	BE11353_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-008	Cytb	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	ACCCCTCCTCATATCCAACCA	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11353_Mgig_Bot2012_COI	TSVA-008	1	BE11353_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	TCTTATTCGTTGGAGACTTTATAACCCT	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11353_Mjap_Gib2022_COI	TSVA-008	1	BE11353_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	AATTTAATTGTGACTAGGCATGGTCTG	146.83795
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11354_Cmae_Bot2011_COI	TSVA-009	1	BE11354_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	ATGAACAGTCTATCCTCCTTTAG	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11354_Hsan_Wil2021_COI	TSVA-009	1	BE11354_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	CCTGGGCCGGTATAGTAGGT	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11354_Mros_Gib2022_COI	TSVA-009	1	BE11354_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11354_Msal_Bot2012_COI	TSVA-009	1	BE11354_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	GYTAGTTCCRATGATGTTAGCTG	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11354_Mstr_Wil2021_COI	TSVA-009	1	BE11354_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	GTAGTGGTAACTACTCATGCTTTA	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11354_Pvir_Dia2013_COI	TSVA-009	1	BE11354_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	CTTAGTGGCATTAATTCGDAATCC	146.83964
BE11354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23899	WGS84	BE11354	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11354_Rhar_Sim2018_COI	TSVA-009	1	BE11354_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11354	Sample	BE11354	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-009	COI	45	BE11354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-009	CTGCTGCTATTGCTCATG	146.83964
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11356_Aamu_Bax2006_COI	TSVA-011	1	BE11356_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	GCACAACCGGGATCTTTACTTC	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11356_Pcan_Bot2011_IGS	TSVA-011	1	BE11356_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-011	IGS	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	CGTAATCCTCAGTACTGGCTA	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11356_Pper_Dia2013_COI	TSVA-011	1	BE11356_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	CTTAGTGGCATTAATTCGDAATCC	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11356_Sspa_Oph2007_COI	TSVA-011	1	BE11356_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	GAAGGCCTTCCGATGATC	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11356_Upin_Bot2011_COI	TSVA-011	1	BE11356_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	CTTTAATTACAGCGTTTTTATTGTTGT	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11356_Vgib_Wil2025_COI	TSVA-011	1	BE11356_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	CCCCTCTTTACAAGTTCTTTAGGGT	146.84188
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11357_Asen_Wil2025_COI	TSVA-012	1	BE11357_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	GTTGTTGTTACAACACATGCTCTTTTG	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11357_Cjap_Sim2018_COI	TSVA-012	1	BE11357_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11357_Esin_And2018_Cyb	TSVA-012	1	BE11357_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-012	Cytb	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	ACCCCTCCTCATATCCAACCA	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11357_Mgig_Bot2012_COI	TSVA-012	1	BE11357_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	TCTTATTCGTTGGAGACTTTATAACCCT	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11357_Mjap_Gib2022_COI	TSVA-012	1	BE11357_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	AATTTAATTGTGACTAGGCATGGTCTG	146.83955
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11358_Cmae_Bot2011_COI	TSVA-013	1	BE11358_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	ATGAACAGTCTATCCTCCTTTAG	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11358_Hsan_Wil2021_COI	TSVA-013	1	BE11358_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	CCTGGGCCGGTATAGTAGGT	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11358_Mros_Gib2022_COI	TSVA-013	1	BE11358_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11358_Msal_Bot2012_COI	TSVA-013	1	BE11358_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	GYTAGTTCCRATGATGTTAGCTG	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11358_Mstr_Wil2021_COI	TSVA-013	1	BE11358_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	GTAGTGGTAACTACTCATGCTTTA	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11358_Pvir_Dia2013_COI	TSVA-013	1	BE11358_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	CTTAGTGGCATTAATTCGDAATCC	146.83785
BE11358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23713	WGS84	BE11358	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11358_Rhar_Sim2018_COI	TSVA-013	1	BE11358_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11358	Sample	BE11358	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-013	COI	45	BE11358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-013	CTGCTGCTATTGCTCATG	146.83785
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11360_Asen_Wil2025_COI	TSVA-015	1	BE11360_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	GTTGTTGTTACAACACATGCTCTTTTG	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11360_Cjap_Sim2018_COI	TSVA-015	1	BE11360_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11360_Esin_And2018_Cyb	TSVA-015	1	BE11360_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-015	Cytb	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	ACCCCTCCTCATATCCAACCA	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11360_Mgig_Bot2012_COI	TSVA-015	1	BE11360_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	TCTTATTCGTTGGAGACTTTATAACCCT	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11360_Mjap_Gib2022_COI	TSVA-015	1	BE11360_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	AATTTAATTGTGACTAGGCATGGTCTG	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11361_Cmae_Bot2011_COI	TSVA-016	1	BE11361_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	ATGAACAGTCTATCCTCCTTTAG	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11361_Hsan_Wil2021_COI	TSVA-016	1	BE11361_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	CCTGGGCCGGTATAGTAGGT	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11361_Mros_Gib2022_COI	TSVA-016	1	BE11361_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11361_Msal_Bot2012_COI	TSVA-016	1	BE11361_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	GYTAGTTCCRATGATGTTAGCTG	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11361_Mstr_Wil2021_COI	TSVA-016	1	BE11361_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	GTAGTGGTAACTACTCATGCTTTA	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11361_Pvir_Dia2013_COI	TSVA-016	1	BE11361_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	CTTAGTGGCATTAATTCGDAATCC	146.83612
BE11361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23815	WGS84	BE11361	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11361_Rhar_Sim2018_COI	TSVA-016	1	BE11361_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11361	Sample	BE11361	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-016	COI	45	BE11361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-016	CTGCTGCTATTGCTCATG	146.83612
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11363_Aamu_Bax2006_COI	TSVA-018	1	BE11363_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	GCACAACCGGGATCTTTACTTC	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11363_Pcan_Bot2011_IGS	TSVA-018	1	BE11363_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-018	IGS	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	CGTAATCCTCAGTACTGGCTA	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11363_Pper_Dia2013_COI	TSVA-018	1	BE11363_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	CTTAGTGGCATTAATTCGDAATCC	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11363_Sspa_Oph2007_COI	TSVA-018	1	BE11363_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	GAAGGCCTTCCGATGATC	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11363_Upin_Bot2011_COI	TSVA-018	1	BE11363_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	CTTTAATTACAGCGTTTTTATTGTTGT	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11363_Vgib_Wil2025_COI	TSVA-018	1	BE11363_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	CCCCTCTTTACAAGTTCTTTAGGGT	146.83475
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11364_Asen_Wil2025_COI	TSVA-019	1	BE11364_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	GTTGTTGTTACAACACATGCTCTTTTG	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11364_Cjap_Sim2018_COI	TSVA-019	1	BE11364_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11364_Esin_And2018_Cyb	TSVA-019	1	BE11364_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-019	Cytb	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	ACCCCTCCTCATATCCAACCA	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11364_Mgig_Bot2012_COI	TSVA-019	1	BE11364_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	TCTTATTCGTTGGAGACTTTATAACCCT	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11364_Mjap_Gib2022_COI	TSVA-019	1	BE11364_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	AATTTAATTGTGACTAGGCATGGTCTG	146.83385
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11365_Cmae_Bot2011_COI	TSVA-020	1	BE11365_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	ATGAACAGTCTATCCTCCTTTAG	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11365_Hsan_Wil2021_COI	TSVA-020	1	BE11365_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	CCTGGGCCGGTATAGTAGGT	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11365_Mros_Gib2022_COI	TSVA-020	1	BE11365_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11365_Msal_Bot2012_COI	TSVA-020	1	BE11365_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	GYTAGTTCCRATGATGTTAGCTG	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11365_Mstr_Wil2021_COI	TSVA-020	1	BE11365_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	GTAGTGGTAACTACTCATGCTTTA	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11365_Pvir_Dia2013_COI	TSVA-020	1	BE11365_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	CTTAGTGGCATTAATTCGDAATCC	146.83638
BE11365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23898	WGS84	BE11365	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11365_Rhar_Sim2018_COI	TSVA-020	1	BE11365_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11365	Sample	BE11365	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-020	COI	45	BE11365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-020	CTGCTGCTATTGCTCATG	146.83638
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11367_Aamu_Bax2006_COI	TSVA-022	1	BE11367_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	GCACAACCGGGATCTTTACTTC	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11367_Pcan_Bot2011_IGS	TSVA-022	1	BE11367_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-022	IGS	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	CGTAATCCTCAGTACTGGCTA	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11367_Pper_Dia2013_COI	TSVA-022	1	BE11367_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	CTTAGTGGCATTAATTCGDAATCC	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11367_Sspa_Oph2007_COI	TSVA-022	1	BE11367_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	GAAGGCCTTCCGATGATC	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11367_Upin_Bot2011_COI	TSVA-022	1	BE11367_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	CTTTAATTACAGCGTTTTTATTGTTGT	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11367_Vgib_Wil2025_COI	TSVA-022	1	BE11367_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	CCCCTCTTTACAAGTTCTTTAGGGT	146.83784
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11368_Asen_Wil2025_COI	TSVA-023	1	BE11368_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	GTTGTTGTTACAACACATGCTCTTTTG	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11368_Cjap_Sim2018_COI	TSVA-023	1	BE11368_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11368_Esin_And2018_Cyb	TSVA-023	1	BE11368_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-023	Cytb	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	ACCCCTCCTCATATCCAACCA	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11368_Mgig_Bot2012_COI	TSVA-023	1	BE11368_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	TCTTATTCGTTGGAGACTTTATAACCCT	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11368_Mjap_Gib2022_COI	TSVA-023	1	BE11368_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	AATTTAATTGTGACTAGGCATGGTCTG	146.83503
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11369_Cmae_Bot2011_COI	TSVA-024	1	BE11369_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	ATGAACAGTCTATCCTCCTTTAG	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11369_Hsan_Wil2021_COI	TSVA-024	1	BE11369_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	CCTGGGCCGGTATAGTAGGT	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11369_Mros_Gib2022_COI	TSVA-024	1	BE11369_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11369_Msal_Bot2012_COI	TSVA-024	1	BE11369_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	GYTAGTTCCRATGATGTTAGCTG	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11369_Mstr_Wil2021_COI	TSVA-024	1	BE11369_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	GTAGTGGTAACTACTCATGCTTTA	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11369_Pvir_Dia2013_COI	TSVA-024	1	BE11369_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	CTTAGTGGCATTAATTCGDAATCC	146.835
BE11369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24516	WGS84	BE11369	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11369_Rhar_Sim2018_COI	TSVA-024	1	BE11369_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11369	Sample	BE11369	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-024	COI	45	BE11369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-024	CTGCTGCTATTGCTCATG	146.835
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11370_Aamu_Bax2006_COI	TSVA-025	1	BE11370_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	GCACAACCGGGATCTTTACTTC	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11370_Pcan_Bot2011_IGS	TSVA-025	1	BE11370_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-025	IGS	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	CGTAATCCTCAGTACTGGCTA	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11370_Pper_Dia2013_COI	TSVA-025	1	BE11370_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	CTTAGTGGCATTAATTCGDAATCC	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11370_Sspa_Oph2007_COI	TSVA-025	1	BE11370_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	GAAGGCCTTCCGATGATC	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11370_Upin_Bot2011_COI	TSVA-025	1	BE11370_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	CTTTAATTACAGCGTTTTTATTGTTGT	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11370_Vgib_Wil2025_COI	TSVA-025	1	BE11370_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	CCCCTCTTTACAAGTTCTTTAGGGT	146.83313
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11371_Asen_Wil2025_COI	TSVA-026	1	BE11371_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	GTTGTTGTTACAACACATGCTCTTTTG	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11371_Cjap_Sim2018_COI	TSVA-026	1	BE11371_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11371_Esin_And2018_Cyb	TSVA-026	1	BE11371_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-026	Cytb	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	ACCCCTCCTCATATCCAACCA	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11371_Mgig_Bot2012_COI	TSVA-026	1	BE11371_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	TCTTATTCGTTGGAGACTTTATAACCCT	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11371_Mjap_Gib2022_COI	TSVA-026	1	BE11371_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	AATTTAATTGTGACTAGGCATGGTCTG	146.83212
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11372_Cmae_Bot2011_COI	TSVA-027	1	BE11372_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	ATGAACAGTCTATCCTCCTTTAG	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11372_Hsan_Wil2021_COI	TSVA-027	1	BE11372_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	CCTGGGCCGGTATAGTAGGT	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11372_Mros_Gib2022_COI	TSVA-027	1	BE11372_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11372_Msal_Bot2012_COI	TSVA-027	1	BE11372_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	GYTAGTTCCRATGATGTTAGCTG	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11372_Mstr_Wil2021_COI	TSVA-027	1	BE11372_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	GTAGTGGTAACTACTCATGCTTTA	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11372_Pvir_Dia2013_COI	TSVA-027	1	BE11372_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	CTTAGTGGCATTAATTCGDAATCC	146.83145
BE11372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24779	WGS84	BE11372	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11372_Rhar_Sim2018_COI	TSVA-027	1	BE11372_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11372	Sample	BE11372	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-027	COI	45	BE11372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-027	CTGCTGCTATTGCTCATG	146.83145
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11374_Aamu_Bax2006_COI	TSVA-029	1	BE11374_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	GCACAACCGGGATCTTTACTTC	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11374_Pcan_Bot2011_IGS	TSVA-029	1	BE11374_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-029	IGS	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	CGTAATCCTCAGTACTGGCTA	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11374_Pper_Dia2013_COI	TSVA-029	1	BE11374_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	CTTAGTGGCATTAATTCGDAATCC	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11374_Sspa_Oph2007_COI	TSVA-029	1	BE11374_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	GAAGGCCTTCCGATGATC	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11374_Upin_Bot2011_COI	TSVA-029	1	BE11374_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	CTTTAATTACAGCGTTTTTATTGTTGT	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11374_Vgib_Wil2025_COI	TSVA-029	1	BE11374_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	CCCCTCTTTACAAGTTCTTTAGGGT	146.83552
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11375_Asen_Wil2025_COI	TSVA-030	1	BE11375_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	GTTGTTGTTACAACACATGCTCTTTTG	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11375_Cjap_Sim2018_COI	TSVA-030	1	BE11375_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11375_Esin_And2018_Cyb	TSVA-030	1	BE11375_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-030	Cytb	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	ACCCCTCCTCATATCCAACCA	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11375_Mgig_Bot2012_COI	TSVA-030	1	BE11375_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	TCTTATTCGTTGGAGACTTTATAACCCT	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11375_Mjap_Gib2022_COI	TSVA-030	1	BE11375_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	AATTTAATTGTGACTAGGCATGGTCTG	146.83475
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11376_Cmae_Bot2011_COI	TSVA-031	1	BE11376_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	ATGAACAGTCTATCCTCCTTTAG	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11376_Hsan_Wil2021_COI	TSVA-031	1	BE11376_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	CCTGGGCCGGTATAGTAGGT	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11376_Mros_Gib2022_COI	TSVA-031	1	BE11376_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11376_Msal_Bot2012_COI	TSVA-031	1	BE11376_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	GYTAGTTCCRATGATGTTAGCTG	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11376_Mstr_Wil2021_COI	TSVA-031	1	BE11376_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	GTAGTGGTAACTACTCATGCTTTA	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11376_Pvir_Dia2013_COI	TSVA-031	1	BE11376_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	CTTAGTGGCATTAATTCGDAATCC	146.83336
BE11376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24794	WGS84	BE11376	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11376_Rhar_Sim2018_COI	TSVA-031	1	BE11376_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11376	Sample	BE11376	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-031	COI	45	BE11376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-031	CTGCTGCTATTGCTCATG	146.83336
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11378_Aamu_Bax2006_COI	TSVA-033	1	BE11378_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	GCACAACCGGGATCTTTACTTC	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11378_Pcan_Bot2011_IGS	TSVA-033	1	BE11378_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-033	IGS	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	CGTAATCCTCAGTACTGGCTA	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11378_Pper_Dia2013_COI	TSVA-033	1	BE11378_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	CTTAGTGGCATTAATTCGDAATCC	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11378_Sspa_Oph2007_COI	TSVA-033	1	BE11378_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	GAAGGCCTTCCGATGATC	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11378_Upin_Bot2011_COI	TSVA-033	1	BE11378_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	CTTTAATTACAGCGTTTTTATTGTTGT	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11378_Vgib_Wil2025_COI	TSVA-033	1	BE11378_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	CCCCTCTTTACAAGTTCTTTAGGGT	146.83416
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11379_Asen_Wil2025_COI	TSVA-034	1	BE11379_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	GTTGTTGTTACAACACATGCTCTTTTG	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11379_Cjap_Sim2018_COI	TSVA-034	1	BE11379_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11379_Esin_And2018_Cyb	TSVA-034	1	BE11379_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-034	Cytb	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	ACCCCTCCTCATATCCAACCA	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11379_Mgig_Bot2012_COI	TSVA-034	1	BE11379_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	TCTTATTCGTTGGAGACTTTATAACCCT	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11379_Mjap_Gib2022_COI	TSVA-034	1	BE11379_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	AATTTAATTGTGACTAGGCATGGTCTG	146.83376
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11385_Aamu_Bax2006_COI	GLTA-001	1	BE11385_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	GCACAACCGGGATCTTTACTTC	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11385_Pcan_Bot2011_IGS	GLTA-001	1	BE11385_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-001	IGS	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	CGTAATCCTCAGTACTGGCTA	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11385_Pper_Dia2013_COI	GLTA-001	1	BE11385_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	CTTAGTGGCATTAATTCGDAATCC	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11385_Sspa_Oph2007_COI	GLTA-001	1	BE11385_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	GAAGGCCTTCCGATGATC	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11385_Upin_Bot2011_COI	GLTA-001	1	BE11385_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	CTTTAATTACAGCGTTTTTATTGTTGT	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11385_Vgib_Wil2025_COI	GLTA-001	1	BE11385_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	CCCCTCTTTACAAGTTCTTTAGGGT	151.32361
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11386_Asen_Wil2025_COI	GLTA-002	1	BE11386_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	GTTGTTGTTACAACACATGCTCTTTTG	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11386_Cjap_Sim2018_COI	GLTA-002	1	BE11386_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11386_Esin_And2018_Cyb	GLTA-002	1	BE11386_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-002	Cytb	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	ACCCCTCCTCATATCCAACCA	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11386_Mgig_Bot2012_COI	GLTA-002	1	BE11386_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	TCTTATTCGTTGGAGACTTTATAACCCT	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11386_Mjap_Gib2022_COI	GLTA-002	1	BE11386_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	AATTTAATTGTGACTAGGCATGGTCTG	151.32328
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11387_Cmae_Bot2011_COI	GLTA-003	1	BE11387_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	ATGAACAGTCTATCCTCCTTTAG	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11387_Hsan_Wil2021_COI	GLTA-003	1	BE11387_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	CCTGGGCCGGTATAGTAGGT	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11387_Mros_Gib2022_COI	GLTA-003	1	BE11387_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11387_Msal_Bot2012_COI	GLTA-003	1	BE11387_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	GYTAGTTCCRATGATGTTAGCTG	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11387_Mstr_Wil2021_COI	GLTA-003	1	BE11387_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	GTAGTGGTAACTACTCATGCTTTA	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11387_Pvir_Dia2013_COI	GLTA-003	1	BE11387_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	CTTAGTGGCATTAATTCGDAATCC	151.32533
BE11387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8557	WGS84	BE11387	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11387_Rhar_Sim2018_COI	GLTA-003	1	BE11387_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11387	Sample	BE11387	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-003	COI	45	BE11387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-003	CTGCTGCTATTGCTCATG	151.32533
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11389_Aamu_Bax2006_COI	GLTA-005	1	BE11389_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	GCACAACCGGGATCTTTACTTC	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11389_Pcan_Bot2011_IGS	GLTA-005	1	BE11389_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-005	IGS	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	CGTAATCCTCAGTACTGGCTA	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11389_Pper_Dia2013_COI	GLTA-005	1	BE11389_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	CTTAGTGGCATTAATTCGDAATCC	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11389_Sspa_Oph2007_COI	GLTA-005	1	BE11389_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	GAAGGCCTTCCGATGATC	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11389_Upin_Bot2011_COI	GLTA-005	1	BE11389_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	CTTTAATTACAGCGTTTTTATTGTTGT	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11389_Vgib_Wil2025_COI	GLTA-005	1	BE11389_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	CCCCTCTTTACAAGTTCTTTAGGGT	151.32614
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11390_Cmae_Bot2011_COI	GLTA-006	1	BE11390_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	ATGAACAGTCTATCCTCCTTTAG	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11390_Hsan_Wil2021_COI	GLTA-006	1	BE11390_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	CCTGGGCCGGTATAGTAGGT	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11390_Mros_Gib2022_COI	GLTA-006	1	BE11390_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11390_Msal_Bot2012_COI	GLTA-006	1	BE11390_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	GYTAGTTCCRATGATGTTAGCTG	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11390_Mstr_Wil2021_COI	GLTA-006	1	BE11390_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	GTAGTGGTAACTACTCATGCTTTA	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11390_Pvir_Dia2013_COI	GLTA-006	1	BE11390_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	CTTAGTGGCATTAATTCGDAATCC	151.32283
BE11390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8535	WGS84	BE11390	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11390_Rhar_Sim2018_COI	GLTA-006	1	BE11390_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11390	Sample	BE11390	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-006	COI	45	BE11390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-006	CTGCTGCTATTGCTCATG	151.32283
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11392_Aamu_Bax2006_COI	GLTA-008	1	BE11392_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	GCACAACCGGGATCTTTACTTC	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11392_Pcan_Bot2011_IGS	GLTA-008	1	BE11392_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-008	IGS	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	CGTAATCCTCAGTACTGGCTA	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11392_Pper_Dia2013_COI	GLTA-008	1	BE11392_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	CTTAGTGGCATTAATTCGDAATCC	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11392_Sspa_Oph2007_COI	GLTA-008	1	BE11392_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	GAAGGCCTTCCGATGATC	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11392_Upin_Bot2011_COI	GLTA-008	1	BE11392_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	CTTTAATTACAGCGTTTTTATTGTTGT	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11392_Vgib_Wil2025_COI	GLTA-008	1	BE11392_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	CCCCTCTTTACAAGTTCTTTAGGGT	151.31684
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11393_Asen_Wil2025_COI	GLTA-009	1	BE11393_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	GTTGTTGTTACAACACATGCTCTTTTG	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11393_Cjap_Sim2018_COI	GLTA-009	1	BE11393_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11393_Esin_And2018_Cyb	GLTA-009	1	BE11393_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-009	Cytb	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	ACCCCTCCTCATATCCAACCA	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11393_Mgig_Bot2012_COI	GLTA-009	1	BE11393_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	TCTTATTCGTTGGAGACTTTATAACCCT	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11393_Mjap_Gib2022_COI	GLTA-009	1	BE11393_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	AATTTAATTGTGACTAGGCATGGTCTG	151.31842
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11394_Cmae_Bot2011_COI	GLTA-010	1	BE11394_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	ATGAACAGTCTATCCTCCTTTAG	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11394_Hsan_Wil2021_COI	GLTA-010	1	BE11394_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	CCTGGGCCGGTATAGTAGGT	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11394_Mros_Gib2022_COI	GLTA-010	1	BE11394_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11394_Msal_Bot2012_COI	GLTA-010	1	BE11394_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	GYTAGTTCCRATGATGTTAGCTG	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11394_Mstr_Wil2021_COI	GLTA-010	1	BE11394_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	GTAGTGGTAACTACTCATGCTTTA	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11394_Pvir_Dia2013_COI	GLTA-010	1	BE11394_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	CTTAGTGGCATTAATTCGDAATCC	151.32062
BE11394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85245	WGS84	BE11394	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11394_Rhar_Sim2018_COI	GLTA-010	1	BE11394_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11394	Sample	BE11394	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-010	COI	45	BE11394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-010	CTGCTGCTATTGCTCATG	151.32062
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11396_Aamu_Bax2006_COI	GLTA-012	1	BE11396_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	GCACAACCGGGATCTTTACTTC	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11396_Pcan_Bot2011_IGS	GLTA-012	1	BE11396_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-012	IGS	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	CGTAATCCTCAGTACTGGCTA	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11396_Pper_Dia2013_COI	GLTA-012	1	BE11396_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	CTTAGTGGCATTAATTCGDAATCC	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11396_Sspa_Oph2007_COI	GLTA-012	1	BE11396_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	GAAGGCCTTCCGATGATC	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11396_Upin_Bot2011_COI	GLTA-012	1	BE11396_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	CTTTAATTACAGCGTTTTTATTGTTGT	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11396_Vgib_Wil2025_COI	GLTA-012	1	BE11396_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	CCCCTCTTTACAAGTTCTTTAGGGT	151.32342
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11397_Asen_Wil2025_COI	GLTA-013	1	BE11397_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	GTTGTTGTTACAACACATGCTCTTTTG	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11397_Cjap_Sim2018_COI	GLTA-013	1	BE11397_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11397_Esin_And2018_Cyb	GLTA-013	1	BE11397_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-013	Cytb	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	ACCCCTCCTCATATCCAACCA	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11397_Mgig_Bot2012_COI	GLTA-013	1	BE11397_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	TCTTATTCGTTGGAGACTTTATAACCCT	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11397_Mjap_Gib2022_COI	GLTA-013	1	BE11397_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	AATTTAATTGTGACTAGGCATGGTCTG	151.3073
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11398_Cmae_Bot2011_COI	GLTA-014	1	BE11398_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	ATGAACAGTCTATCCTCCTTTAG	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11398_Hsan_Wil2021_COI	GLTA-014	1	BE11398_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	CCTGGGCCGGTATAGTAGGT	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11398_Mros_Gib2022_COI	GLTA-014	1	BE11398_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11398_Msal_Bot2012_COI	GLTA-014	1	BE11398_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	GYTAGTTCCRATGATGTTAGCTG	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11398_Mstr_Wil2021_COI	GLTA-014	1	BE11398_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	GTAGTGGTAACTACTCATGCTTTA	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11398_Pvir_Dia2013_COI	GLTA-014	1	BE11398_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	CTTAGTGGCATTAATTCGDAATCC	151.31563
BE11398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85152	WGS84	BE11398	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11398_Rhar_Sim2018_COI	GLTA-014	1	BE11398_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11398	Sample	BE11398	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-014	COI	45	BE11398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-014	CTGCTGCTATTGCTCATG	151.31563
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11400_Aamu_Bax2006_COI	GLTA-016	1	BE11400_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	GCACAACCGGGATCTTTACTTC	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11400_Pcan_Bot2011_IGS	GLTA-016	1	BE11400_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-016	IGS	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	CGTAATCCTCAGTACTGGCTA	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11400_Pper_Dia2013_COI	GLTA-016	1	BE11400_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	CTTAGTGGCATTAATTCGDAATCC	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11400_Sspa_Oph2007_COI	GLTA-016	1	BE11400_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	GAAGGCCTTCCGATGATC	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11400_Upin_Bot2011_COI	GLTA-016	1	BE11400_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	CTTTAATTACAGCGTTTTTATTGTTGT	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11400_Vgib_Wil2025_COI	GLTA-016	1	BE11400_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	CCCCTCTTTACAAGTTCTTTAGGGT	151.31914
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11401_Asen_Wil2025_COI	GLTA-017	1	BE11401_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	GTTGTTGTTACAACACATGCTCTTTTG	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11401_Cjap_Sim2018_COI	GLTA-017	1	BE11401_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11401_Esin_And2018_Cyb	GLTA-017	1	BE11401_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-017	Cytb	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	ACCCCTCCTCATATCCAACCA	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11401_Mgig_Bot2012_COI	GLTA-017	1	BE11401_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	TCTTATTCGTTGGAGACTTTATAACCCT	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11401_Mjap_Gib2022_COI	GLTA-017	1	BE11401_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	AATTTAATTGTGACTAGGCATGGTCTG	151.3209
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11402_Cmae_Bot2011_COI	GLTA-018	1	BE11402_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	ATGAACAGTCTATCCTCCTTTAG	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11402_Hsan_Wil2021_COI	GLTA-018	1	BE11402_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	CCTGGGCCGGTATAGTAGGT	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11402_Mros_Gib2022_COI	GLTA-018	1	BE11402_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11402_Msal_Bot2012_COI	GLTA-018	1	BE11402_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	GYTAGTTCCRATGATGTTAGCTG	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11402_Mstr_Wil2021_COI	GLTA-018	1	BE11402_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	GTAGTGGTAACTACTCATGCTTTA	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11402_Pvir_Dia2013_COI	GLTA-018	1	BE11402_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	CTTAGTGGCATTAATTCGDAATCC	151.32283
BE11402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85079	WGS84	BE11402	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11402_Rhar_Sim2018_COI	GLTA-018	1	BE11402_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11402	Sample	BE11402	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-018	COI	45	BE11402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-018	CTGCTGCTATTGCTCATG	151.32283
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11404_Aamu_Bax2006_COI	GLTA-020	1	BE11404_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	GCACAACCGGGATCTTTACTTC	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11404_Pcan_Bot2011_IGS	GLTA-020	1	BE11404_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-020	IGS	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	CGTAATCCTCAGTACTGGCTA	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11404_Pper_Dia2013_COI	GLTA-020	1	BE11404_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	CTTAGTGGCATTAATTCGDAATCC	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11404_Sspa_Oph2007_COI	GLTA-020	1	BE11404_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	GAAGGCCTTCCGATGATC	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11404_Upin_Bot2011_COI	GLTA-020	1	BE11404_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	CTTTAATTACAGCGTTTTTATTGTTGT	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11404_Vgib_Wil2025_COI	GLTA-020	1	BE11404_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	CCCCTCTTTACAAGTTCTTTAGGGT	151.308
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11405_Asen_Wil2025_COI	GLTA-021	1	BE11405_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	GTTGTTGTTACAACACATGCTCTTTTG	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11405_Cjap_Sim2018_COI	GLTA-021	1	BE11405_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11405_Esin_And2018_Cyb	GLTA-021	1	BE11405_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-021	Cytb	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	ACCCCTCCTCATATCCAACCA	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11405_Mgig_Bot2012_COI	GLTA-021	1	BE11405_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	TCTTATTCGTTGGAGACTTTATAACCCT	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11405_Mjap_Gib2022_COI	GLTA-021	1	BE11405_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	AATTTAATTGTGACTAGGCATGGTCTG	151.30947
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11406_Cmae_Bot2011_COI	GLTA-022	1	BE11406_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	ATGAACAGTCTATCCTCCTTTAG	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11406_Hsan_Wil2021_COI	GLTA-022	1	BE11406_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	CCTGGGCCGGTATAGTAGGT	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11406_Mros_Gib2022_COI	GLTA-022	1	BE11406_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11406_Msal_Bot2012_COI	GLTA-022	1	BE11406_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	GYTAGTTCCRATGATGTTAGCTG	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11406_Mstr_Wil2021_COI	GLTA-022	1	BE11406_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	GTAGTGGTAACTACTCATGCTTTA	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11406_Pvir_Dia2013_COI	GLTA-022	1	BE11406_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	CTTAGTGGCATTAATTCGDAATCC	151.31087
BE11406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85066	WGS84	BE11406	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11406_Rhar_Sim2018_COI	GLTA-022	1	BE11406_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11406	Sample	BE11406	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-022	COI	45	BE11406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-022	CTGCTGCTATTGCTCATG	151.31087
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11408_Aamu_Bax2006_COI	GLTA-024	1	BE11408_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	GCACAACCGGGATCTTTACTTC	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11408_Pcan_Bot2011_IGS	GLTA-024	1	BE11408_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-024	IGS	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	CGTAATCCTCAGTACTGGCTA	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11408_Pper_Dia2013_COI	GLTA-024	1	BE11408_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	CTTAGTGGCATTAATTCGDAATCC	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11408_Sspa_Oph2007_COI	GLTA-024	1	BE11408_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	GAAGGCCTTCCGATGATC	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11408_Upin_Bot2011_COI	GLTA-024	1	BE11408_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	CTTTAATTACAGCGTTTTTATTGTTGT	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11408_Vgib_Wil2025_COI	GLTA-024	1	BE11408_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	CCCCTCTTTACAAGTTCTTTAGGGT	151.31454
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11409_Asen_Wil2025_COI	GLTA-025	1	BE11409_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	GTTGTTGTTACAACACATGCTCTTTTG	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11409_Cjap_Sim2018_COI	GLTA-025	1	BE11409_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11409_Esin_And2018_Cyb	GLTA-025	1	BE11409_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-025	Cytb	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	ACCCCTCCTCATATCCAACCA	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11409_Mgig_Bot2012_COI	GLTA-025	1	BE11409_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	TCTTATTCGTTGGAGACTTTATAACCCT	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11409_Mjap_Gib2022_COI	GLTA-025	1	BE11409_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	AATTTAATTGTGACTAGGCATGGTCTG	151.3163
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11411_Aamu_Bax2006_COI	GLTA-027	1	BE11411_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	GCACAACCGGGATCTTTACTTC	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11411_Pcan_Bot2011_IGS	GLTA-027	1	BE11411_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-027	IGS	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	CGTAATCCTCAGTACTGGCTA	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11411_Pper_Dia2013_COI	GLTA-027	1	BE11411_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	CTTAGTGGCATTAATTCGDAATCC	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11411_Sspa_Oph2007_COI	GLTA-027	1	BE11411_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	GAAGGCCTTCCGATGATC	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11411_Upin_Bot2011_COI	GLTA-027	1	BE11411_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	CTTTAATTACAGCGTTTTTATTGTTGT	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11411_Vgib_Wil2025_COI	GLTA-027	1	BE11411_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	CCCCTCTTTACAAGTTCTTTAGGGT	151.31994
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11412_Asen_Wil2025_COI	GLTA-028	1	BE11412_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	GTTGTTGTTACAACACATGCTCTTTTG	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11412_Cjap_Sim2018_COI	GLTA-028	1	BE11412_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11412_Esin_And2018_Cyb	GLTA-028	1	BE11412_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-028	Cytb	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	ACCCCTCCTCATATCCAACCA	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11412_Mgig_Bot2012_COI	GLTA-028	1	BE11412_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	TCTTATTCGTTGGAGACTTTATAACCCT	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11412_Mjap_Gib2022_COI	GLTA-028	1	BE11412_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	AATTTAATTGTGACTAGGCATGGTCTG	151.30704
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11413_Cmae_Bot2011_COI	GLTA-029	1	BE11413_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	ATGAACAGTCTATCCTCCTTTAG	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11413_Hsan_Wil2021_COI	GLTA-029	1	BE11413_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	CCTGGGCCGGTATAGTAGGT	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11413_Mros_Gib2022_COI	GLTA-029	1	BE11413_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11413_Msal_Bot2012_COI	GLTA-029	1	BE11413_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	GYTAGTTCCRATGATGTTAGCTG	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11413_Mstr_Wil2021_COI	GLTA-029	1	BE11413_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	GTAGTGGTAACTACTCATGCTTTA	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11413_Pvir_Dia2013_COI	GLTA-029	1	BE11413_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	CTTAGTGGCATTAATTCGDAATCC	151.3087
BE11413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84979	WGS84	BE11413	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11413_Rhar_Sim2018_COI	GLTA-029	1	BE11413_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11413	Sample	BE11413	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-029	COI	45	BE11413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-029	CTGCTGCTATTGCTCATG	151.3087
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11415_Aamu_Bax2006_COI	GLTA-031	1	BE11415_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	GCACAACCGGGATCTTTACTTC	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11415_Pcan_Bot2011_IGS	GLTA-031	1	BE11415_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-031	IGS	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	CGTAATCCTCAGTACTGGCTA	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11415_Pper_Dia2013_COI	GLTA-031	1	BE11415_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	CTTAGTGGCATTAATTCGDAATCC	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11415_Sspa_Oph2007_COI	GLTA-031	1	BE11415_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	GAAGGCCTTCCGATGATC	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11415_Upin_Bot2011_COI	GLTA-031	1	BE11415_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	CTTTAATTACAGCGTTTTTATTGTTGT	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11415_Vgib_Wil2025_COI	GLTA-031	1	BE11415_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	CCCCTCTTTACAAGTTCTTTAGGGT	151.3121
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11416_Asen_Wil2025_COI	GLTA-032	1	BE11416_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	GTTGTTGTTACAACACATGCTCTTTTG	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11416_Cjap_Sim2018_COI	GLTA-032	1	BE11416_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11416_Esin_And2018_Cyb	GLTA-032	1	BE11416_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-032	Cytb	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	ACCCCTCCTCATATCCAACCA	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11416_Mgig_Bot2012_COI	GLTA-032	1	BE11416_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	TCTTATTCGTTGGAGACTTTATAACCCT	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11416_Mjap_Gib2022_COI	GLTA-032	1	BE11416_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	AATTTAATTGTGACTAGGCATGGTCTG	151.31387
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11417_Cmae_Bot2011_COI	GLTA-033	1	BE11417_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	ATGAACAGTCTATCCTCCTTTAG	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11417_Mros_Gib2022_COI	GLTA-033	1	BE11417_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11417_Msal_Bot2012_COI	GLTA-033	1	BE11417_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	GYTAGTTCCRATGATGTTAGCTG	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11417_Mstr_Wil2021_COI	GLTA-033	1	BE11417_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	GTAGTGGTAACTACTCATGCTTTA	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11417_Pvir_Dia2013_COI	GLTA-033	1	BE11417_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	CTTAGTGGCATTAATTCGDAATCC	151.3157
BE11417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84889	WGS84	BE11417	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11417_Rhar_Sim2018_COI	GLTA-033	1	BE11417_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11417	Sample	BE11417	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-033	COI	45	BE11417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-033	CTGCTGCTATTGCTCATG	151.3157
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11419_Aamu_Bax2006_COI	GLTA-035	1	BE11419_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	GCACAACCGGGATCTTTACTTC	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11419_Pcan_Bot2011_IGS	GLTA-035	1	BE11419_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-035	IGS	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	CGTAATCCTCAGTACTGGCTA	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11419_Pper_Dia2013_COI	GLTA-035	1	BE11419_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	CTTAGTGGCATTAATTCGDAATCC	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11419_Sspa_Oph2007_COI	GLTA-035	1	BE11419_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	GAAGGCCTTCCGATGATC	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11419_Upin_Bot2011_COI	GLTA-035	1	BE11419_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	CTTTAATTACAGCGTTTTTATTGTTGT	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11419_Vgib_Wil2025_COI	GLTA-035	1	BE11419_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	CCCCTCTTTACAAGTTCTTTAGGGT	151.31275
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11424_Cmae_Bot2011_COI	GLTA-036	1	BE11424_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	ATGAACAGTCTATCCTCCTTTAG	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11424_Hsan_Wil2021_COI	GLTA-036	1	BE11424_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	CCTGGGCCGGTATAGTAGGT	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11424_Mros_Gib2022_COI	GLTA-036	1	BE11424_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11424_Msal_Bot2012_COI	GLTA-036	1	BE11424_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	GYTAGTTCCRATGATGTTAGCTG	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11424_Mstr_Wil2021_COI	GLTA-036	1	BE11424_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	GTAGTGGTAACTACTCATGCTTTA	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11424_Pvir_Dia2013_COI	GLTA-036	1	BE11424_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	CTTAGTGGCATTAATTCGDAATCC	151.27517
BE11424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83955	WGS84	BE11424	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11424_Rhar_Sim2018_COI	GLTA-036	1	BE11424_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11424	Sample	BE11424	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-036	COI	45	BE11424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-036	CTGCTGCTATTGCTCATG	151.27517
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11426_Aamu_Bax2006_COI	GLTA-038	1	BE11426_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	GCACAACCGGGATCTTTACTTC	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11426_Pcan_Bot2011_IGS	GLTA-038	1	BE11426_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-038	IGS	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	CGTAATCCTCAGTACTGGCTA	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11426_Pper_Dia2013_COI	GLTA-038	1	BE11426_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	CTTAGTGGCATTAATTCGDAATCC	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11426_Sspa_Oph2007_COI	GLTA-038	1	BE11426_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	GAAGGCCTTCCGATGATC	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11426_Upin_Bot2011_COI	GLTA-038	1	BE11426_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	CTTTAATTACAGCGTTTTTATTGTTGT	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11426_Vgib_Wil2025_COI	GLTA-038	1	BE11426_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	CCCCTCTTTACAAGTTCTTTAGGGT	151.27728
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11427_Asen_Wil2025_COI	GLTA-039	1	BE11427_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	GTTGTTGTTACAACACATGCTCTTTTG	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11427_Cjap_Sim2018_COI	GLTA-039	1	BE11427_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11427_Esin_And2018_Cyb	GLTA-039	1	BE11427_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-039	Cytb	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	ACCCCTCCTCATATCCAACCA	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11427_Mgig_Bot2012_COI	GLTA-039	1	BE11427_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	TCTTATTCGTTGGAGACTTTATAACCCT	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11427_Mjap_Gib2022_COI	GLTA-039	1	BE11427_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	AATTTAATTGTGACTAGGCATGGTCTG	151.27652
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11428_Cmae_Bot2011_COI	GLTA-040	1	BE11428_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	ATGAACAGTCTATCCTCCTTTAG	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11428_Hsan_Wil2021_COI	GLTA-040	1	BE11428_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	CCTGGGCCGGTATAGTAGGT	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11428_Mros_Gib2022_COI	GLTA-040	1	BE11428_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11428_Msal_Bot2012_COI	GLTA-040	1	BE11428_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	GYTAGTTCCRATGATGTTAGCTG	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11428_Mstr_Wil2021_COI	GLTA-040	1	BE11428_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	GTAGTGGTAACTACTCATGCTTTA	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11428_Pvir_Dia2013_COI	GLTA-040	1	BE11428_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	CTTAGTGGCATTAATTCGDAATCC	151.2752
BE11428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83487	WGS84	BE11428	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11428_Rhar_Sim2018_COI	GLTA-040	1	BE11428_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11428	Sample	BE11428	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-040	COI	45	BE11428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-040	CTGCTGCTATTGCTCATG	151.2752
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11430_Asen_Wil2025_COI	GLTA-042	1	BE11430_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	GTTGTTGTTACAACACATGCTCTTTTG	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11430_Cjap_Sim2018_COI	GLTA-042	1	BE11430_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11430_Esin_And2018_Cyb	GLTA-042	1	BE11430_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-042	Cytb	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	ACCCCTCCTCATATCCAACCA	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11430_Mgig_Bot2012_COI	GLTA-042	1	BE11430_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	TCTTATTCGTTGGAGACTTTATAACCCT	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11430_Mjap_Gib2022_COI	GLTA-042	1	BE11430_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	AATTTAATTGTGACTAGGCATGGTCTG	151.26694
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11431_Cmae_Bot2011_COI	GLTA-043	1	BE11431_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	ATGAACAGTCTATCCTCCTTTAG	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11431_Hsan_Wil2021_COI	GLTA-043	1	BE11431_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	CCTGGGCCGGTATAGTAGGT	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11431_Mros_Gib2022_COI	GLTA-043	1	BE11431_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11431_Msal_Bot2012_COI	GLTA-043	1	BE11431_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	GYTAGTTCCRATGATGTTAGCTG	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11431_Mstr_Wil2021_COI	GLTA-043	1	BE11431_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	GTAGTGGTAACTACTCATGCTTTA	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11431_Pvir_Dia2013_COI	GLTA-043	1	BE11431_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	CTTAGTGGCATTAATTCGDAATCC	151.26895
BE11431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83403	WGS84	BE11431	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11431_Rhar_Sim2018_COI	GLTA-043	1	BE11431_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11431	Sample	BE11431	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-043	COI	45	BE11431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-043	CTGCTGCTATTGCTCATG	151.26895
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11433_Aamu_Bax2006_COI	GLTA-045	1	BE11433_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	GCACAACCGGGATCTTTACTTC	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11433_Pcan_Bot2011_IGS	GLTA-045	1	BE11433_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-045	IGS	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	CGTAATCCTCAGTACTGGCTA	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11433_Pper_Dia2013_COI	GLTA-045	1	BE11433_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	CTTAGTGGCATTAATTCGDAATCC	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11433_Sspa_Oph2007_COI	GLTA-045	1	BE11433_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	GAAGGCCTTCCGATGATC	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11433_Upin_Bot2011_COI	GLTA-045	1	BE11433_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	CTTTAATTACAGCGTTTTTATTGTTGT	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11433_Vgib_Wil2025_COI	GLTA-045	1	BE11433_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	CCCCTCTTTACAAGTTCTTTAGGGT	151.27266
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11434_Asen_Wil2025_COI	GLTA-046	1	BE11434_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	GTTGTTGTTACAACACATGCTCTTTTG	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11434_Cjap_Sim2018_COI	GLTA-046	1	BE11434_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11434_Esin_And2018_Cyb	GLTA-046	1	BE11434_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-046	Cytb	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	ACCCCTCCTCATATCCAACCA	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11434_Mgig_Bot2012_COI	GLTA-046	1	BE11434_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	TCTTATTCGTTGGAGACTTTATAACCCT	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11434_Mjap_Gib2022_COI	GLTA-046	1	BE11434_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	AATTTAATTGTGACTAGGCATGGTCTG	151.27417
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11435_Cmae_Bot2011_COI	GLTA-047	1	BE11435_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	ATGAACAGTCTATCCTCCTTTAG	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11435_Hsan_Wil2021_COI	GLTA-047	1	BE11435_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	CCTGGGCCGGTATAGTAGGT	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11435_Mros_Gib2022_COI	GLTA-047	1	BE11435_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11435_Msal_Bot2012_COI	GLTA-047	1	BE11435_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	GYTAGTTCCRATGATGTTAGCTG	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11435_Mstr_Wil2021_COI	GLTA-047	1	BE11435_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	GTAGTGGTAACTACTCATGCTTTA	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11435_Pvir_Dia2013_COI	GLTA-047	1	BE11435_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	CTTAGTGGCATTAATTCGDAATCC	151.27718
BE11435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83304	WGS84	BE11435	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11435_Rhar_Sim2018_COI	GLTA-047	1	BE11435_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11435	Sample	BE11435	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-047	COI	45	BE11435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-047	CTGCTGCTATTGCTCATG	151.27718
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11437_Aamu_Bax2006_COI	GLTA-049	1	BE11437_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	GCACAACCGGGATCTTTACTTC	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11437_Pcan_Bot2011_IGS	GLTA-049	1	BE11437_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-049	IGS	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	CGTAATCCTCAGTACTGGCTA	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11437_Pper_Dia2013_COI	GLTA-049	1	BE11437_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	CTTAGTGGCATTAATTCGDAATCC	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11437_Sspa_Oph2007_COI	GLTA-049	1	BE11437_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	GAAGGCCTTCCGATGATC	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11437_Upin_Bot2011_COI	GLTA-049	1	BE11437_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	CTTTAATTACAGCGTTTTTATTGTTGT	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11437_Vgib_Wil2025_COI	GLTA-049	1	BE11437_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	CCCCTCTTTACAAGTTCTTTAGGGT	151.26763
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11438_Asen_Wil2025_COI	GLTA-050	1	BE11438_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	GTTGTTGTTACAACACATGCTCTTTTG	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11438_Cjap_Sim2018_COI	GLTA-050	1	BE11438_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11438_Esin_And2018_Cyb	GLTA-050	1	BE11438_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-050	Cytb	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	ACCCCTCCTCATATCCAACCA	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11438_Mgig_Bot2012_COI	GLTA-050	1	BE11438_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	TCTTATTCGTTGGAGACTTTATAACCCT	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11438_Mjap_Gib2022_COI	GLTA-050	1	BE11438_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	AATTTAATTGTGACTAGGCATGGTCTG	151.26955
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11439_Cmae_Bot2011_COI	GLTA-051	1	BE11439_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	ATGAACAGTCTATCCTCCTTTAG	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11439_Hsan_Wil2021_COI	GLTA-051	1	BE11439_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	CCTGGGCCGGTATAGTAGGT	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11439_Mros_Gib2022_COI	GLTA-051	1	BE11439_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11439_Msal_Bot2012_COI	GLTA-051	1	BE11439_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	GYTAGTTCCRATGATGTTAGCTG	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11439_Mstr_Wil2021_COI	GLTA-051	1	BE11439_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	GTAGTGGTAACTACTCATGCTTTA	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11439_Pvir_Dia2013_COI	GLTA-051	1	BE11439_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	CTTAGTGGCATTAATTCGDAATCC	151.27186
BE11439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83247	WGS84	BE11439	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11439_Rhar_Sim2018_COI	GLTA-051	1	BE11439_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11439	Sample	BE11439	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-051	COI	45	BE11439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-051	CTGCTGCTATTGCTCATG	151.27186
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11440_Aamu_Bax2006_COI	GLTA-052	1	BE11440_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	GCACAACCGGGATCTTTACTTC	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11440_Pcan_Bot2011_IGS	GLTA-052	1	BE11440_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-052	IGS	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	CGTAATCCTCAGTACTGGCTA	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11440_Pper_Dia2013_COI	GLTA-052	1	BE11440_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	CTTAGTGGCATTAATTCGDAATCC	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11440_Sspa_Oph2007_COI	GLTA-052	1	BE11440_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	GAAGGCCTTCCGATGATC	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11440_Upin_Bot2011_COI	GLTA-052	1	BE11440_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	CTTTAATTACAGCGTTTTTATTGTTGT	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11440_Vgib_Wil2025_COI	GLTA-052	1	BE11440_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	CCCCTCTTTACAAGTTCTTTAGGGT	151.27336
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11441_Asen_Wil2025_COI	GLTA-053	1	BE11441_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	GTTGTTGTTACAACACATGCTCTTTTG	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11441_Cjap_Sim2018_COI	GLTA-053	1	BE11441_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11441_Esin_And2018_Cyb	GLTA-053	1	BE11441_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-053	Cytb	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	ACCCCTCCTCATATCCAACCA	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11441_Mgig_Bot2012_COI	GLTA-053	1	BE11441_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	TCTTATTCGTTGGAGACTTTATAACCCT	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11441_Mjap_Gib2022_COI	GLTA-053	1	BE11441_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	AATTTAATTGTGACTAGGCATGGTCTG	151.26337
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11442_Cmae_Bot2011_COI	GLTA-054	1	BE11442_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	ATGAACAGTCTATCCTCCTTTAG	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11442_Hsan_Wil2021_COI	GLTA-054	1	BE11442_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	CCTGGGCCGGTATAGTAGGT	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11442_Mros_Gib2022_COI	GLTA-054	1	BE11442_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11442_Msal_Bot2012_COI	GLTA-054	1	BE11442_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	GYTAGTTCCRATGATGTTAGCTG	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11442_Mstr_Wil2021_COI	GLTA-054	1	BE11442_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	GTAGTGGTAACTACTCATGCTTTA	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11442_Pvir_Dia2013_COI	GLTA-054	1	BE11442_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	CTTAGTGGCATTAATTCGDAATCC	151.26466
BE11442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8317	WGS84	BE11442	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11442_Rhar_Sim2018_COI	GLTA-054	1	BE11442_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11442	Sample	BE11442	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-054	COI	45	BE11442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-054	CTGCTGCTATTGCTCATG	151.26466
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11444_Aamu_Bax2006_COI	GLTA-056	1	BE11444_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	GCACAACCGGGATCTTTACTTC	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11444_Pcan_Bot2011_IGS	GLTA-056	1	BE11444_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-056	IGS	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	CGTAATCCTCAGTACTGGCTA	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11444_Pper_Dia2013_COI	GLTA-056	1	BE11444_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	CTTAGTGGCATTAATTCGDAATCC	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11444_Sspa_Oph2007_COI	GLTA-056	1	BE11444_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	GAAGGCCTTCCGATGATC	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11444_Upin_Bot2011_COI	GLTA-056	1	BE11444_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	CTTTAATTACAGCGTTTTTATTGTTGT	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11444_Vgib_Wil2025_COI	GLTA-056	1	BE11444_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	CCCCTCTTTACAAGTTCTTTAGGGT	151.26801
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11445_Asen_Wil2025_COI	GLTA-057	1	BE11445_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	GTTGTTGTTACAACACATGCTCTTTTG	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11445_Cjap_Sim2018_COI	GLTA-057	1	BE11445_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11445_Esin_And2018_Cyb	GLTA-057	1	BE11445_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-057	Cytb	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	ACCCCTCCTCATATCCAACCA	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11445_Mgig_Bot2012_COI	GLTA-057	1	BE11445_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	TCTTATTCGTTGGAGACTTTATAACCCT	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11445_Mjap_Gib2022_COI	GLTA-057	1	BE11445_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	AATTTAATTGTGACTAGGCATGGTCTG	151.25797
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11446_Cmae_Bot2011_COI	GLTA-058	1	BE11446_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	ATGAACAGTCTATCCTCCTTTAG	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11446_Hsan_Wil2021_COI	GLTA-058	1	BE11446_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	CCTGGGCCGGTATAGTAGGT	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11446_Mros_Gib2022_COI	GLTA-058	1	BE11446_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11446_Msal_Bot2012_COI	GLTA-058	1	BE11446_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	GYTAGTTCCRATGATGTTAGCTG	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11446_Mstr_Wil2021_COI	GLTA-058	1	BE11446_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	GTAGTGGTAACTACTCATGCTTTA	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11446_Pvir_Dia2013_COI	GLTA-058	1	BE11446_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	CTTAGTGGCATTAATTCGDAATCC	151.25936
BE11446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83046	WGS84	BE11446	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11446_Rhar_Sim2018_COI	GLTA-058	1	BE11446_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11446	Sample	BE11446	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-058	COI	45	BE11446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-058	CTGCTGCTATTGCTCATG	151.25936
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11448_Aamu_Bax2006_COI	GLTA-060	1	BE11448_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	GCACAACCGGGATCTTTACTTC	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11448_Pcan_Bot2011_IGS	GLTA-060	1	BE11448_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-060	IGS	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	CGTAATCCTCAGTACTGGCTA	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11448_Pper_Dia2013_COI	GLTA-060	1	BE11448_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	CTTAGTGGCATTAATTCGDAATCC	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11448_Sspa_Oph2007_COI	GLTA-060	1	BE11448_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	GAAGGCCTTCCGATGATC	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11448_Upin_Bot2011_COI	GLTA-060	1	BE11448_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	CTTTAATTACAGCGTTTTTATTGTTGT	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11448_Vgib_Wil2025_COI	GLTA-060	1	BE11448_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	CCCCTCTTTACAAGTTCTTTAGGGT	151.26294
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11449_Asen_Wil2025_COI	GLTA-061	1	BE11449_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	GTTGTTGTTACAACACATGCTCTTTTG	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11449_Cjap_Sim2018_COI	GLTA-061	1	BE11449_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11449_Esin_And2018_Cyb	GLTA-061	1	BE11449_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-061	Cytb	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	ACCCCTCCTCATATCCAACCA	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11449_Mgig_Bot2012_COI	GLTA-061	1	BE11449_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	TCTTATTCGTTGGAGACTTTATAACCCT	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11449_Mjap_Gib2022_COI	GLTA-061	1	BE11449_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	AATTTAATTGTGACTAGGCATGGTCTG	151.26484
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11451_Aamu_Bax2006_COI	GLTA-063	1	BE11451_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	GCACAACCGGGATCTTTACTTC	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11451_Pcan_Bot2011_IGS	GLTA-063	1	BE11451_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-063	IGS	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	CGTAATCCTCAGTACTGGCTA	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11451_Pper_Dia2013_COI	GLTA-063	1	BE11451_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	CTTAGTGGCATTAATTCGDAATCC	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11451_Sspa_Oph2007_COI	GLTA-063	1	BE11451_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	GAAGGCCTTCCGATGATC	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11451_Upin_Bot2011_COI	GLTA-063	1	BE11451_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	CTTTAATTACAGCGTTTTTATTGTTGT	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11451_Vgib_Wil2025_COI	GLTA-063	1	BE11451_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	CCCCTCTTTACAAGTTCTTTAGGGT	151.25459
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11452_Asen_Wil2025_COI	GLTA-064	1	BE11452_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	GTTGTTGTTACAACACATGCTCTTTTG	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11452_Cjap_Sim2018_COI	GLTA-064	1	BE11452_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11452_Esin_And2018_Cyb	GLTA-064	1	BE11452_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-064	Cytb	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	ACCCCTCCTCATATCCAACCA	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11452_Mgig_Bot2012_COI	GLTA-064	1	BE11452_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	TCTTATTCGTTGGAGACTTTATAACCCT	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11452_Mjap_Gib2022_COI	GLTA-064	1	BE11452_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	AATTTAATTGTGACTAGGCATGGTCTG	151.25594
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11453_Cmae_Bot2011_COI	GLTA-065	1	BE11453_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	ATGAACAGTCTATCCTCCTTTAG	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11453_Hsan_Wil2021_COI	GLTA-065	1	BE11453_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	CCTGGGCCGGTATAGTAGGT	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11453_Mros_Gib2022_COI	GLTA-065	1	BE11453_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11453_Msal_Bot2012_COI	GLTA-065	1	BE11453_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	GYTAGTTCCRATGATGTTAGCTG	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11453_Mstr_Wil2021_COI	GLTA-065	1	BE11453_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	GTAGTGGTAACTACTCATGCTTTA	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11453_Pvir_Dia2013_COI	GLTA-065	1	BE11453_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	CTTAGTGGCATTAATTCGDAATCC	151.25786
BE11453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82926	WGS84	BE11453	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11453_Rhar_Sim2018_COI	GLTA-065	1	BE11453_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11453	Sample	BE11453	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-065	COI	45	BE11453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-065	CTGCTGCTATTGCTCATG	151.25786
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11455_Aamu_Bax2006_COI	GLTA-067	1	BE11455_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	GCACAACCGGGATCTTTACTTC	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11455_Pcan_Bot2011_IGS	GLTA-067	1	BE11455_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-067	IGS	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	CGTAATCCTCAGTACTGGCTA	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11455_Pper_Dia2013_COI	GLTA-067	1	BE11455_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	CTTAGTGGCATTAATTCGDAATCC	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11455_Sspa_Oph2007_COI	GLTA-067	1	BE11455_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	GAAGGCCTTCCGATGATC	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11455_Upin_Bot2011_COI	GLTA-067	1	BE11455_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	CTTTAATTACAGCGTTTTTATTGTTGT	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11455_Vgib_Wil2025_COI	GLTA-067	1	BE11455_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	CCCCTCTTTACAAGTTCTTTAGGGT	151.26156
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11456_Asen_Wil2025_COI	GLTA-068	1	BE11456_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	GTTGTTGTTACAACACATGCTCTTTTG	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11456_Cjap_Sim2018_COI	GLTA-068	1	BE11456_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11456_Esin_And2018_Cyb	GLTA-068	1	BE11456_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-068	Cytb	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	ACCCCTCCTCATATCCAACCA	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11456_Mgig_Bot2012_COI	GLTA-068	1	BE11456_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	TCTTATTCGTTGGAGACTTTATAACCCT	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11456_Mjap_Gib2022_COI	GLTA-068	1	BE11456_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	AATTTAATTGTGACTAGGCATGGTCTG	151.25335
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11457_Cmae_Bot2011_COI	GLTA-069	1	BE11457_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	ATGAACAGTCTATCCTCCTTTAG	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11457_Hsan_Wil2021_COI	GLTA-069	1	BE11457_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	CCTGGGCCGGTATAGTAGGT	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11457_Mros_Gib2022_COI	GLTA-069	1	BE11457_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11457_Msal_Bot2012_COI	GLTA-069	1	BE11457_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	GYTAGTTCCRATGATGTTAGCTG	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11457_Mstr_Wil2021_COI	GLTA-069	1	BE11457_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	GTAGTGGTAACTACTCATGCTTTA	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11457_Pvir_Dia2013_COI	GLTA-069	1	BE11457_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	CTTAGTGGCATTAATTCGDAATCC	151.25516
BE11457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82827	WGS84	BE11457	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11457_Rhar_Sim2018_COI	GLTA-069	1	BE11457_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11457	Sample	BE11457	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-069	COI	45	BE11457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-069	CTGCTGCTATTGCTCATG	151.25516
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11463_Asen_Wil2025_COI	GLTA-076	1	BE11463_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	GTTGTTGTTACAACACATGCTCTTTTG	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11463_Cjap_Sim2018_COI	GLTA-076	1	BE11463_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11463_Esin_And2018_Cyb	GLTA-076	1	BE11463_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-076	Cytb	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	ACCCCTCCTCATATCCAACCA	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11463_Mgig_Bot2012_COI	GLTA-076	1	BE11463_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	TCTTATTCGTTGGAGACTTTATAACCCT	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11463_Mjap_Gib2022_COI	GLTA-076	1	BE11463_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	AATTTAATTGTGACTAGGCATGGTCTG	151.18532
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11464_Cmae_Bot2011_COI	GLTA-077	1	BE11464_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	ATGAACAGTCTATCCTCCTTTAG	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11464_Hsan_Wil2021_COI	GLTA-077	1	BE11464_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	CCTGGGCCGGTATAGTAGGT	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11464_Mros_Gib2022_COI	GLTA-077	1	BE11464_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11464_Msal_Bot2012_COI	GLTA-077	1	BE11464_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	GYTAGTTCCRATGATGTTAGCTG	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11464_Mstr_Wil2021_COI	GLTA-077	1	BE11464_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	GTAGTGGTAACTACTCATGCTTTA	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11464_Pvir_Dia2013_COI	GLTA-077	1	BE11464_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	CTTAGTGGCATTAATTCGDAATCC	151.18717
BE11464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79331	WGS84	BE11464	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11464_Rhar_Sim2018_COI	GLTA-077	1	BE11464_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11464	Sample	BE11464	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-077	COI	45	BE11464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-077	CTGCTGCTATTGCTCATG	151.18717
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11466_Aamu_Bax2006_COI	GLTA-079	1	BE11466_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	GCACAACCGGGATCTTTACTTC	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11466_Pcan_Bot2011_IGS	GLTA-079	1	BE11466_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-079	IGS	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	CGTAATCCTCAGTACTGGCTA	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11466_Pper_Dia2013_COI	GLTA-079	1	BE11466_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	CTTAGTGGCATTAATTCGDAATCC	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11466_Sspa_Oph2007_COI	GLTA-079	1	BE11466_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	GAAGGCCTTCCGATGATC	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11466_Upin_Bot2011_COI	GLTA-079	1	BE11466_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	CTTTAATTACAGCGTTTTTATTGTTGT	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11466_Vgib_Wil2025_COI	GLTA-079	1	BE11466_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	CCCCTCTTTACAAGTTCTTTAGGGT	151.18113
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11467_Asen_Wil2025_COI	GLTA-080	1	BE11467_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	GTTGTTGTTACAACACATGCTCTTTTG	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11467_Cjap_Sim2018_COI	GLTA-080	1	BE11467_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11467_Esin_And2018_Cyb	GLTA-080	1	BE11467_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-080	Cytb	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	ACCCCTCCTCATATCCAACCA	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11467_Mgig_Bot2012_COI	GLTA-080	1	BE11467_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	TCTTATTCGTTGGAGACTTTATAACCCT	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11467_Mjap_Gib2022_COI	GLTA-080	1	BE11467_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	AATTTAATTGTGACTAGGCATGGTCTG	151.18602
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11468_Cmae_Bot2011_COI	GLTA-081	1	BE11468_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	ATGAACAGTCTATCCTCCTTTAG	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11468_Hsan_Wil2021_COI	GLTA-081	1	BE11468_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	CCTGGGCCGGTATAGTAGGT	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11468_Mros_Gib2022_COI	GLTA-081	1	BE11468_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11468_Msal_Bot2012_COI	GLTA-081	1	BE11468_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	GYTAGTTCCRATGATGTTAGCTG	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11468_Mstr_Wil2021_COI	GLTA-081	1	BE11468_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	GTAGTGGTAACTACTCATGCTTTA	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11468_Pvir_Dia2013_COI	GLTA-081	1	BE11468_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	CTTAGTGGCATTAATTCGDAATCC	151.18752
BE11468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79172	WGS84	BE11468	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11468_Rhar_Sim2018_COI	GLTA-081	1	BE11468_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11468	Sample	BE11468	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-081	COI	45	BE11468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-081	CTGCTGCTATTGCTCATG	151.18752
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11470_Asen_Wil2025_COI	GLTA-083	1	BE11470_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	GTTGTTGTTACAACACATGCTCTTTTG	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11470_Cjap_Sim2018_COI	GLTA-083	1	BE11470_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11470_Esin_And2018_Cyb	GLTA-083	1	BE11470_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-083	Cytb	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	ACCCCTCCTCATATCCAACCA	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11470_Mgig_Bot2012_COI	GLTA-083	1	BE11470_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	TCTTATTCGTTGGAGACTTTATAACCCT	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11470_Mjap_Gib2022_COI	GLTA-083	1	BE11470_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	AATTTAATTGTGACTAGGCATGGTCTG	151.18476
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11471_Cmae_Bot2011_COI	GLTA-084	1	BE11471_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	ATGAACAGTCTATCCTCCTTTAG	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11471_Hsan_Wil2021_COI	GLTA-084	1	BE11471_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	CCTGGGCCGGTATAGTAGGT	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11471_Mros_Gib2022_COI	GLTA-084	1	BE11471_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11471_Msal_Bot2012_COI	GLTA-084	1	BE11471_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	GYTAGTTCCRATGATGTTAGCTG	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11471_Mstr_Wil2021_COI	GLTA-084	1	BE11471_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	GTAGTGGTAACTACTCATGCTTTA	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11471_Pvir_Dia2013_COI	GLTA-084	1	BE11471_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	CTTAGTGGCATTAATTCGDAATCC	151.18654
BE11471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79061	WGS84	BE11471	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11471_Rhar_Sim2018_COI	GLTA-084	1	BE11471_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11471	Sample	BE11471	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-084	COI	45	BE11471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-084	CTGCTGCTATTGCTCATG	151.18654
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11473_Aamu_Bax2006_COI	GLTA-086	1	BE11473_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	GCACAACCGGGATCTTTACTTC	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11473_Pcan_Bot2011_IGS	GLTA-086	1	BE11473_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-086	IGS	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	CGTAATCCTCAGTACTGGCTA	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11473_Pper_Dia2013_COI	GLTA-086	1	BE11473_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	CTTAGTGGCATTAATTCGDAATCC	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11473_Sspa_Oph2007_COI	GLTA-086	1	BE11473_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	GAAGGCCTTCCGATGATC	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11473_Upin_Bot2011_COI	GLTA-086	1	BE11473_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	CTTTAATTACAGCGTTTTTATTGTTGT	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11473_Vgib_Wil2025_COI	GLTA-086	1	BE11473_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	CCCCTCTTTACAAGTTCTTTAGGGT	151.18417
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11474_Asen_Wil2025_COI	GLTA-087	1	BE11474_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	GTTGTTGTTACAACACATGCTCTTTTG	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11474_Cjap_Sim2018_COI	GLTA-087	1	BE11474_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11474_Esin_And2018_Cyb	GLTA-087	1	BE11474_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-087	Cytb	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	ACCCCTCCTCATATCCAACCA	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11474_Mgig_Bot2012_COI	GLTA-087	1	BE11474_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	TCTTATTCGTTGGAGACTTTATAACCCT	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11474_Mjap_Gib2022_COI	GLTA-087	1	BE11474_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	AATTTAATTGTGACTAGGCATGGTCTG	151.18564
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11475_Cmae_Bot2011_COI	GLTA-088	1	BE11475_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	ATGAACAGTCTATCCTCCTTTAG	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11475_Hsan_Wil2021_COI	GLTA-088	1	BE11475_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	CCTGGGCCGGTATAGTAGGT	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11475_Mros_Gib2022_COI	GLTA-088	1	BE11475_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11475_Msal_Bot2012_COI	GLTA-088	1	BE11475_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	GYTAGTTCCRATGATGTTAGCTG	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11475_Mstr_Wil2021_COI	GLTA-088	1	BE11475_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	GTAGTGGTAACTACTCATGCTTTA	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11475_Pvir_Dia2013_COI	GLTA-088	1	BE11475_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	CTTAGTGGCATTAATTCGDAATCC	151.18114
BE11475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78882	WGS84	BE11475	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11475_Rhar_Sim2018_COI	GLTA-088	1	BE11475_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11475	Sample	BE11475	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-088	COI	45	BE11475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-088	CTGCTGCTATTGCTCATG	151.18114
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11477_Aamu_Bax2006_COI	GLTA-090	1	BE11477_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	GCACAACCGGGATCTTTACTTC	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11477_Pcan_Bot2011_IGS	GLTA-090	1	BE11477_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-090	IGS	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	CGTAATCCTCAGTACTGGCTA	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11477_Pper_Dia2013_COI	GLTA-090	1	BE11477_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	CTTAGTGGCATTAATTCGDAATCC	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11477_Sspa_Oph2007_COI	GLTA-090	1	BE11477_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	GAAGGCCTTCCGATGATC	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11477_Upin_Bot2011_COI	GLTA-090	1	BE11477_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	CTTTAATTACAGCGTTTTTATTGTTGT	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11477_Vgib_Wil2025_COI	GLTA-090	1	BE11477_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	CCCCTCTTTACAAGTTCTTTAGGGT	151.18425
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11478_Asen_Wil2025_COI	GLTA-091	1	BE11478_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	GTTGTTGTTACAACACATGCTCTTTTG	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11478_Cjap_Sim2018_COI	GLTA-091	1	BE11478_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11478_Esin_And2018_Cyb	GLTA-091	1	BE11478_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-091	Cytb	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	ACCCCTCCTCATATCCAACCA	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11478_Mgig_Bot2012_COI	GLTA-091	1	BE11478_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	TCTTATTCGTTGGAGACTTTATAACCCT	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11478_Mjap_Gib2022_COI	GLTA-091	1	BE11478_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	AATTTAATTGTGACTAGGCATGGTCTG	151.1787
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11479_Cmae_Bot2011_COI	GLTA-092	1	BE11479_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	ATGAACAGTCTATCCTCCTTTAG	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11479_Hsan_Wil2021_COI	GLTA-092	1	BE11479_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	CCTGGGCCGGTATAGTAGGT	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11479_Mros_Gib2022_COI	GLTA-092	1	BE11479_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11479_Msal_Bot2012_COI	GLTA-092	1	BE11479_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	GYTAGTTCCRATGATGTTAGCTG	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11479_Mstr_Wil2021_COI	GLTA-092	1	BE11479_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	GTAGTGGTAACTACTCATGCTTTA	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11479_Pvir_Dia2013_COI	GLTA-092	1	BE11479_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	CTTAGTGGCATTAATTCGDAATCC	151.1796
BE11479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7876	WGS84	BE11479	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11479_Rhar_Sim2018_COI	GLTA-092	1	BE11479_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11479	Sample	BE11479	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-092	COI	45	BE11479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-092	CTGCTGCTATTGCTCATG	151.1796
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11480_Aamu_Bax2006_COI	GLTA-093	1	BE11480_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	GCACAACCGGGATCTTTACTTC	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11480_Pcan_Bot2011_IGS	GLTA-093	1	BE11480_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-093	IGS	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	CGTAATCCTCAGTACTGGCTA	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11480_Pper_Dia2013_COI	GLTA-093	1	BE11480_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	CTTAGTGGCATTAATTCGDAATCC	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11480_Sspa_Oph2007_COI	GLTA-093	1	BE11480_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	GAAGGCCTTCCGATGATC	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11480_Upin_Bot2011_COI	GLTA-093	1	BE11480_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	CTTTAATTACAGCGTTTTTATTGTTGT	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11480_Vgib_Wil2025_COI	GLTA-093	1	BE11480_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	CCCCTCTTTACAAGTTCTTTAGGGT	151.18133
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11481_Asen_Wil2025_COI	GLTA-094	1	BE11481_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	GTTGTTGTTACAACACATGCTCTTTTG	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11481_Cjap_Sim2018_COI	GLTA-094	1	BE11481_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11481_Esin_And2018_Cyb	GLTA-094	1	BE11481_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-094	Cytb	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	ACCCCTCCTCATATCCAACCA	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11481_Mgig_Bot2012_COI	GLTA-094	1	BE11481_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	TCTTATTCGTTGGAGACTTTATAACCCT	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11481_Mjap_Gib2022_COI	GLTA-094	1	BE11481_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	AATTTAATTGTGACTAGGCATGGTCTG	151.1775
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11482_Cmae_Bot2011_COI	GLTA-095	1	BE11482_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	ATGAACAGTCTATCCTCCTTTAG	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11482_Hsan_Wil2021_COI	GLTA-095	1	BE11482_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	CCTGGGCCGGTATAGTAGGT	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11482_Mros_Gib2022_COI	GLTA-095	1	BE11482_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11482_Msal_Bot2012_COI	GLTA-095	1	BE11482_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	GYTAGTTCCRATGATGTTAGCTG	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11482_Mstr_Wil2021_COI	GLTA-095	1	BE11482_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	GTAGTGGTAACTACTCATGCTTTA	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11482_Pvir_Dia2013_COI	GLTA-095	1	BE11482_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	CTTAGTGGCATTAATTCGDAATCC	151.17867
BE11482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78652	WGS84	BE11482	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11482_Rhar_Sim2018_COI	GLTA-095	1	BE11482_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11482	Sample	BE11482	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-095	COI	45	BE11482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-095	CTGCTGCTATTGCTCATG	151.17867
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11484_Aamu_Bax2006_COI	GLTA-097	1	BE11484_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	GCACAACCGGGATCTTTACTTC	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11484_Pcan_Bot2011_IGS	GLTA-097	1	BE11484_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-097	IGS	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	CGTAATCCTCAGTACTGGCTA	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11484_Pper_Dia2013_COI	GLTA-097	1	BE11484_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	CTTAGTGGCATTAATTCGDAATCC	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11484_Sspa_Oph2007_COI	GLTA-097	1	BE11484_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	GAAGGCCTTCCGATGATC	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11484_Upin_Bot2011_COI	GLTA-097	1	BE11484_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	CTTTAATTACAGCGTTTTTATTGTTGT	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11484_Vgib_Wil2025_COI	GLTA-097	1	BE11484_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	CCCCTCTTTACAAGTTCTTTAGGGT	151.17661
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11485_Asen_Wil2025_COI	GLTA-098	1	BE11485_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	GTTGTTGTTACAACACATGCTCTTTTG	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11485_Cjap_Sim2018_COI	GLTA-098	1	BE11485_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11485_Esin_And2018_Cyb	GLTA-098	1	BE11485_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-098	Cytb	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	ACCCCTCCTCATATCCAACCA	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11485_Mgig_Bot2012_COI	GLTA-098	1	BE11485_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	TCTTATTCGTTGGAGACTTTATAACCCT	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11485_Mjap_Gib2022_COI	GLTA-098	1	BE11485_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	AATTTAATTGTGACTAGGCATGGTCTG	151.17817
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11486_Cmae_Bot2011_COI	GLTA-099	1	BE11486_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	ATGAACAGTCTATCCTCCTTTAG	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11486_Hsan_Wil2021_COI	GLTA-099	1	BE11486_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	CCTGGGCCGGTATAGTAGGT	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11486_Mros_Gib2022_COI	GLTA-099	1	BE11486_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11486_Msal_Bot2012_COI	GLTA-099	1	BE11486_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	GYTAGTTCCRATGATGTTAGCTG	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11486_Mstr_Wil2021_COI	GLTA-099	1	BE11486_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	GTAGTGGTAACTACTCATGCTTTA	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11486_Pvir_Dia2013_COI	GLTA-099	1	BE11486_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	CTTAGTGGCATTAATTCGDAATCC	151.1738
BE11486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78428	WGS84	BE11486	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11486_Rhar_Sim2018_COI	GLTA-099	1	BE11486_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11486	Sample	BE11486	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-099	COI	45	BE11486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-099	CTGCTGCTATTGCTCATG	151.1738
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11488_Aamu_Bax2006_COI	GLTA-101	1	BE11488_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	GCACAACCGGGATCTTTACTTC	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11488_Pcan_Bot2011_IGS	GLTA-101	1	BE11488_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-101	IGS	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	CGTAATCCTCAGTACTGGCTA	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11488_Pper_Dia2013_COI	GLTA-101	1	BE11488_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	CTTAGTGGCATTAATTCGDAATCC	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11488_Sspa_Oph2007_COI	GLTA-101	1	BE11488_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	GAAGGCCTTCCGATGATC	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11488_Upin_Bot2011_COI	GLTA-101	1	BE11488_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	CTTTAATTACAGCGTTTTTATTGTTGT	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11488_Vgib_Wil2025_COI	GLTA-101	1	BE11488_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	CCCCTCTTTACAAGTTCTTTAGGGT	151.17702
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11489_Asen_Wil2025_COI	GLTA-102	1	BE11489_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	GTTGTTGTTACAACACATGCTCTTTTG	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11489_Cjap_Sim2018_COI	GLTA-102	1	BE11489_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11489_Esin_And2018_Cyb	GLTA-102	1	BE11489_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-102	Cytb	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	ACCCCTCCTCATATCCAACCA	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11489_Mgig_Bot2012_COI	GLTA-102	1	BE11489_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	TCTTATTCGTTGGAGACTTTATAACCCT	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11489_Mjap_Gib2022_COI	GLTA-102	1	BE11489_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	AATTTAATTGTGACTAGGCATGGTCTG	151.17267
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11491_Aamu_Bax2006_COI	GLTA-104	1	BE11491_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	GCACAACCGGGATCTTTACTTC	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11491_Pcan_Bot2011_IGS	GLTA-104	1	BE11491_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-104	IGS	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	CGTAATCCTCAGTACTGGCTA	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11491_Pper_Dia2013_COI	GLTA-104	1	BE11491_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	CTTAGTGGCATTAATTCGDAATCC	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11491_Sspa_Oph2007_COI	GLTA-104	1	BE11491_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	GAAGGCCTTCCGATGATC	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11491_Upin_Bot2011_COI	GLTA-104	1	BE11491_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	CTTTAATTACAGCGTTTTTATTGTTGT	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11491_Vgib_Wil2025_COI	GLTA-104	1	BE11491_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	CCCCTCTTTACAAGTTCTTTAGGGT	151.17602
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11492_Asen_Wil2025_COI	GLTA-105	1	BE11492_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	GTTGTTGTTACAACACATGCTCTTTTG	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11492_Cjap_Sim2018_COI	GLTA-105	1	BE11492_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11492_Esin_And2018_Cyb	GLTA-105	1	BE11492_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-105	Cytb	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	ACCCCTCCTCATATCCAACCA	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11492_Mgig_Bot2012_COI	GLTA-105	1	BE11492_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	TCTTATTCGTTGGAGACTTTATAACCCT	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11492_Mjap_Gib2022_COI	GLTA-105	1	BE11492_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	AATTTAATTGTGACTAGGCATGGTCTG	151.17294
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11497_Cmae_Bot2011_COI	GLTA-071	1	BE11497_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	ATGAACAGTCTATCCTCCTTTAG	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11497_Hsan_Wil2021_COI	GLTA-071	1	BE11497_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	CCTGGGCCGGTATAGTAGGT	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11497_Mros_Gib2022_COI	GLTA-071	1	BE11497_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11497_Msal_Bot2012_COI	GLTA-071	1	BE11497_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	GYTAGTTCCRATGATGTTAGCTG	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11497_Mstr_Wil2021_COI	GLTA-071	1	BE11497_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	GTAGTGGTAACTACTCATGCTTTA	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11497_Pvir_Dia2013_COI	GLTA-071	1	BE11497_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	CTTAGTGGCATTAATTCGDAATCC	151.1845
BE11497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79656	WGS84	BE11497	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11497_Rhar_Sim2018_COI	GLTA-071	1	BE11497_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11497	Sample	BE11497	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-071	COI	45	BE11497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-071	CTGCTGCTATTGCTCATG	151.1845
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11499_Aamu_Bax2006_COI	GLTA-073	1	BE11499_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	GCACAACCGGGATCTTTACTTC	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11499_Pcan_Bot2011_IGS	GLTA-073	1	BE11499_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-073	IGS	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	CGTAATCCTCAGTACTGGCTA	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11499_Pper_Dia2013_COI	GLTA-073	1	BE11499_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	CTTAGTGGCATTAATTCGDAATCC	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11499_Sspa_Oph2007_COI	GLTA-073	1	BE11499_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	GAAGGCCTTCCGATGATC	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11499_Upin_Bot2011_COI	GLTA-073	1	BE11499_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	CTTTAATTACAGCGTTTTTATTGTTGT	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11499_Vgib_Wil2025_COI	GLTA-073	1	BE11499_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	CCCCTCTTTACAAGTTCTTTAGGGT	151.18617
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11500_Asen_Wil2025_COI	GLTA-074	1	BE11500_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	GTTGTTGTTACAACACATGCTCTTTTG	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11500_Cjap_Sim2018_COI	GLTA-074	1	BE11500_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11500_Esin_And2018_Cyb	GLTA-074	1	BE11500_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-074	Cytb	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	ACCCCTCCTCATATCCAACCA	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11500_Mgig_Bot2012_COI	GLTA-074	1	BE11500_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	TCTTATTCGTTGGAGACTTTATAACCCT	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11500_Mjap_Gib2022_COI	GLTA-074	1	BE11500_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	AATTTAATTGTGACTAGGCATGGTCTG	151.18815
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11501_Cmae_Bot2011_COI	GLTA-075	1	BE11501_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	ATGAACAGTCTATCCTCCTTTAG	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11501_Hsan_Wil2021_COI	GLTA-075	1	BE11501_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	CCTGGGCCGGTATAGTAGGT	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11501_Mros_Gib2022_COI	GLTA-075	1	BE11501_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11501_Msal_Bot2012_COI	GLTA-075	1	BE11501_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	GYTAGTTCCRATGATGTTAGCTG	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11501_Mstr_Wil2021_COI	GLTA-075	1	BE11501_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	GTAGTGGTAACTACTCATGCTTTA	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11501_Pvir_Dia2013_COI	GLTA-075	1	BE11501_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	CTTAGTGGCATTAATTCGDAATCC	151.1821
BE11501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79407	WGS84	BE11501	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11501_Rhar_Sim2018_COI	GLTA-075	1	BE11501_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11501	Sample	BE11501	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-075	COI	45	BE11501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-075	CTGCTGCTATTGCTCATG	151.1821
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11503_Aamu_Bax2006_COI	DRWA-002	1	BE11503_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	GCACAACCGGGATCTTTACTTC	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11503_Pcan_Bot2011_IGS	DRWA-002	1	BE11503_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-002	IGS	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	CGTAATCCTCAGTACTGGCTA	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11503_Pper_Dia2013_COI	DRWA-002	1	BE11503_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	CTTAGTGGCATTAATTCGDAATCC	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11503_Sspa_Oph2007_COI	DRWA-002	1	BE11503_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	GAAGGCCTTCCGATGATC	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11503_Upin_Bot2011_COI	DRWA-002	1	BE11503_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	CTTTAATTACAGCGTTTTTATTGTTGT	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11503_Vgib_Wil2025_COI	DRWA-002	1	BE11503_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	CCCCTCTTTACAAGTTCTTTAGGGT	130.84915
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11504_Asen_Wil2025_COI	DRWA-003	1	BE11504_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	GTTGTTGTTACAACACATGCTCTTTTG	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11504_Cjap_Sim2018_COI	DRWA-003	1	BE11504_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11504_Esin_And2018_Cyb	DRWA-003	1	BE11504_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-003	Cytb	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	ACCCCTCCTCATATCCAACCA	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11504_Mgig_Bot2012_COI	DRWA-003	1	BE11504_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	TCTTATTCGTTGGAGACTTTATAACCCT	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11504_Mjap_Gib2022_COI	DRWA-003	1	BE11504_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	AATTTAATTGTGACTAGGCATGGTCTG	130.85052
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11505_Cmae_Bot2011_COI	DRWA-004	1	BE11505_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	ATGAACAGTCTATCCTCCTTTAG	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11505_Hsan_Wil2021_COI	DRWA-004	1	BE11505_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	CCTGGGCCGGTATAGTAGGT	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11505_Mros_Gib2022_COI	DRWA-004	1	BE11505_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11505_Msal_Bot2012_COI	DRWA-004	1	BE11505_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	GYTAGTTCCRATGATGTTAGCTG	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11505_Mstr_Wil2021_COI	DRWA-004	1	BE11505_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	GTAGTGGTAACTACTCATGCTTTA	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11505_Pvir_Dia2013_COI	DRWA-004	1	BE11505_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	CTTAGTGGCATTAATTCGDAATCC	130.852
BE11505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52836	WGS84	BE11505	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11505_Rhar_Sim2018_COI	DRWA-004	1	BE11505_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11505	Sample	BE11505	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-004	COI	45	BE11505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-004	CTGCTGCTATTGCTCATG	130.852
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11507_Aamu_Bax2006_COI	DRWA-006	1	BE11507_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	GCACAACCGGGATCTTTACTTC	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11507_Pcan_Bot2011_IGS	DRWA-006	1	BE11507_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-006	IGS	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	CGTAATCCTCAGTACTGGCTA	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11507_Pper_Dia2013_COI	DRWA-006	1	BE11507_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	CTTAGTGGCATTAATTCGDAATCC	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11507_Sspa_Oph2007_COI	DRWA-006	1	BE11507_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	GAAGGCCTTCCGATGATC	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11507_Upin_Bot2011_COI	DRWA-006	1	BE11507_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	CTTTAATTACAGCGTTTTTATTGTTGT	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11507_Vgib_Wil2025_COI	DRWA-006	1	BE11507_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	CCCCTCTTTACAAGTTCTTTAGGGT	130.84666
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11508_Asen_Wil2025_COI	DRWA-007	1	BE11508_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	GTTGTTGTTACAACACATGCTCTTTTG	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11508_Cjap_Sim2018_COI	DRWA-007	1	BE11508_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11508_Esin_And2018_Cyb	DRWA-007	1	BE11508_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-007	Cytb	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	ACCCCTCCTCATATCCAACCA	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11508_Mgig_Bot2012_COI	DRWA-007	1	BE11508_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	TCTTATTCGTTGGAGACTTTATAACCCT	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11508_Mjap_Gib2022_COI	DRWA-007	1	BE11508_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	AATTTAATTGTGACTAGGCATGGTCTG	130.84857
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11509_Cmae_Bot2011_COI	DRWA-008	1	BE11509_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	ATGAACAGTCTATCCTCCTTTAG	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11509_Hsan_Wil2021_COI	DRWA-008	1	BE11509_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	CCTGGGCCGGTATAGTAGGT	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11509_Mros_Gib2022_COI	DRWA-008	1	BE11509_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11509_Msal_Bot2012_COI	DRWA-008	1	BE11509_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	GYTAGTTCCRATGATGTTAGCTG	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11509_Mstr_Wil2021_COI	DRWA-008	1	BE11509_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	GTAGTGGTAACTACTCATGCTTTA	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11509_Pvir_Dia2013_COI	DRWA-008	1	BE11509_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	CTTAGTGGCATTAATTCGDAATCC	130.84983
BE11509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11509	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11509_Rhar_Sim2018_COI	DRWA-008	1	BE11509_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11509	Sample	BE11509	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-008	COI	45	BE11509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-008	CTGCTGCTATTGCTCATG	130.84983
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11510_Aamu_Bax2006_COI	DRWA-009	1	BE11510_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	GCACAACCGGGATCTTTACTTC	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11510_Pcan_Bot2011_IGS	DRWA-009	1	BE11510_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-009	IGS	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	CGTAATCCTCAGTACTGGCTA	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11510_Pper_Dia2013_COI	DRWA-009	1	BE11510_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	CTTAGTGGCATTAATTCGDAATCC	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11510_Sspa_Oph2007_COI	DRWA-009	1	BE11510_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	GAAGGCCTTCCGATGATC	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11510_Upin_Bot2011_COI	DRWA-009	1	BE11510_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	CTTTAATTACAGCGTTTTTATTGTTGT	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11510_Vgib_Wil2025_COI	DRWA-009	1	BE11510_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	CCCCTCTTTACAAGTTCTTTAGGGT	130.85118
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11511_Asen_Wil2025_COI	DRWA-010	1	BE11511_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	GTTGTTGTTACAACACATGCTCTTTTG	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11511_Cjap_Sim2018_COI	DRWA-010	1	BE11511_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11511_Esin_And2018_Cyb	DRWA-010	1	BE11511_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-010	Cytb	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	ACCCCTCCTCATATCCAACCA	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11511_Mgig_Bot2012_COI	DRWA-010	1	BE11511_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	TCTTATTCGTTGGAGACTTTATAACCCT	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11511_Mjap_Gib2022_COI	DRWA-010	1	BE11511_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	AATTTAATTGTGACTAGGCATGGTCTG	130.85253
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11512_Cmae_Bot2011_COI	DRWA-011	1	BE11512_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	ATGAACAGTCTATCCTCCTTTAG	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11512_Hsan_Wil2021_COI	DRWA-011	1	BE11512_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	CCTGGGCCGGTATAGTAGGT	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11512_Mros_Gib2022_COI	DRWA-011	1	BE11512_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11512_Msal_Bot2012_COI	DRWA-011	1	BE11512_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	GYTAGTTCCRATGATGTTAGCTG	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11512_Mstr_Wil2021_COI	DRWA-011	1	BE11512_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	GTAGTGGTAACTACTCATGCTTTA	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11512_Pvir_Dia2013_COI	DRWA-011	1	BE11512_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	CTTAGTGGCATTAATTCGDAATCC	130.85392
BE11512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52723	WGS84	BE11512	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11512_Rhar_Sim2018_COI	DRWA-011	1	BE11512_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11512	Sample	BE11512	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-011	COI	45	BE11512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-011	CTGCTGCTATTGCTCATG	130.85392
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11514_Aamu_Bax2006_COI	DRWA-013	1	BE11514_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	GCACAACCGGGATCTTTACTTC	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11514_Pcan_Bot2011_IGS	DRWA-013	1	BE11514_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-013	IGS	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	CGTAATCCTCAGTACTGGCTA	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11514_Pper_Dia2013_COI	DRWA-013	1	BE11514_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	CTTAGTGGCATTAATTCGDAATCC	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11514_Sspa_Oph2007_COI	DRWA-013	1	BE11514_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	GAAGGCCTTCCGATGATC	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11514_Upin_Bot2011_COI	DRWA-013	1	BE11514_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	CTTTAATTACAGCGTTTTTATTGTTGT	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11514_Vgib_Wil2025_COI	DRWA-013	1	BE11514_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	CCCCTCTTTACAAGTTCTTTAGGGT	130.84737
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11515_Asen_Wil2025_COI	DRWA-014	1	BE11515_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	GTTGTTGTTACAACACATGCTCTTTTG	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11515_Cjap_Sim2018_COI	DRWA-014	1	BE11515_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11515_Esin_And2018_Cyb	DRWA-014	1	BE11515_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-014	Cytb	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	ACCCCTCCTCATATCCAACCA	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11515_Mgig_Bot2012_COI	DRWA-014	1	BE11515_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	TCTTATTCGTTGGAGACTTTATAACCCT	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11515_Mjap_Gib2022_COI	DRWA-014	1	BE11515_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	AATTTAATTGTGACTAGGCATGGTCTG	130.84539
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11516_Cmae_Bot2011_COI	DRWA-015	1	BE11516_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	ATGAACAGTCTATCCTCCTTTAG	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11516_Hsan_Wil2021_COI	DRWA-015	1	BE11516_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	CCTGGGCCGGTATAGTAGGT	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11516_Mros_Gib2022_COI	DRWA-015	1	BE11516_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11516_Msal_Bot2012_COI	DRWA-015	1	BE11516_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	GYTAGTTCCRATGATGTTAGCTG	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11516_Mstr_Wil2021_COI	DRWA-015	1	BE11516_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	GTAGTGGTAACTACTCATGCTTTA	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11516_Pvir_Dia2013_COI	DRWA-015	1	BE11516_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	CTTAGTGGCATTAATTCGDAATCC	130.84672
BE11516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52468	WGS84	BE11516	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11516_Rhar_Sim2018_COI	DRWA-015	1	BE11516_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11516	Sample	BE11516	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-015	COI	45	BE11516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-015	CTGCTGCTATTGCTCATG	130.84672
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11518_Aamu_Bax2006_COI	DRWA-017	1	BE11518_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	GCACAACCGGGATCTTTACTTC	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11518_Pcan_Bot2011_IGS	DRWA-017	1	BE11518_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-017	IGS	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	CGTAATCCTCAGTACTGGCTA	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11518_Pper_Dia2013_COI	DRWA-017	1	BE11518_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	CTTAGTGGCATTAATTCGDAATCC	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11518_Sspa_Oph2007_COI	DRWA-017	1	BE11518_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	GAAGGCCTTCCGATGATC	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11518_Upin_Bot2011_COI	DRWA-017	1	BE11518_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	CTTTAATTACAGCGTTTTTATTGTTGT	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11518_Vgib_Wil2025_COI	DRWA-017	1	BE11518_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	CCCCTCTTTACAAGTTCTTTAGGGT	130.846
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11519_Asen_Wil2025_COI	DRWA-018	1	BE11519_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	GTTGTTGTTACAACACATGCTCTTTTG	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11519_Cjap_Sim2018_COI	DRWA-018	1	BE11519_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11519_Esin_And2018_Cyb	DRWA-018	1	BE11519_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-018	Cytb	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	ACCCCTCCTCATATCCAACCA	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11519_Mgig_Bot2012_COI	DRWA-018	1	BE11519_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	TCTTATTCGTTGGAGACTTTATAACCCT	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11519_Mjap_Gib2022_COI	DRWA-018	1	BE11519_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	AATTTAATTGTGACTAGGCATGGTCTG	130.84534
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11521_Aamu_Bax2006_COI	DRWA-020	1	BE11521_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	GCACAACCGGGATCTTTACTTC	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11521_Pcan_Bot2011_IGS	DRWA-020	1	BE11521_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-020	IGS	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	CGTAATCCTCAGTACTGGCTA	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11521_Pper_Dia2013_COI	DRWA-020	1	BE11521_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	CTTAGTGGCATTAATTCGDAATCC	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11521_Sspa_Oph2007_COI	DRWA-020	1	BE11521_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	GAAGGCCTTCCGATGATC	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11521_Upin_Bot2011_COI	DRWA-020	1	BE11521_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	CTTTAATTACAGCGTTTTTATTGTTGT	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11521_Vgib_Wil2025_COI	DRWA-020	1	BE11521_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	CCCCTCTTTACAAGTTCTTTAGGGT	130.84479
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11522_Asen_Wil2025_COI	DRWA-021	1	BE11522_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	GTTGTTGTTACAACACATGCTCTTTTG	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11522_Cjap_Sim2018_COI	DRWA-021	1	BE11522_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11522_Esin_And2018_Cyb	DRWA-021	1	BE11522_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-021	Cytb	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	ACCCCTCCTCATATCCAACCA	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11522_Mgig_Bot2012_COI	DRWA-021	1	BE11522_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	TCTTATTCGTTGGAGACTTTATAACCCT	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11522_Mjap_Gib2022_COI	DRWA-021	1	BE11522_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	AATTTAATTGTGACTAGGCATGGTCTG	130.84612
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11523_Cmae_Bot2011_COI	DRWA-022	1	BE11523_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	ATGAACAGTCTATCCTCCTTTAG	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11523_Hsan_Wil2021_COI	DRWA-022	1	BE11523_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	CCTGGGCCGGTATAGTAGGT	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11523_Mros_Gib2022_COI	DRWA-022	1	BE11523_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11523_Msal_Bot2012_COI	DRWA-022	1	BE11523_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	GYTAGTTCCRATGATGTTAGCTG	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11523_Mstr_Wil2021_COI	DRWA-022	1	BE11523_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	GTAGTGGTAACTACTCATGCTTTA	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11523_Pvir_Dia2013_COI	DRWA-022	1	BE11523_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	CTTAGTGGCATTAATTCGDAATCC	130.84736
BE11523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52131	WGS84	BE11523	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11523_Rhar_Sim2018_COI	DRWA-022	1	BE11523_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11523	Sample	BE11523	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-022	COI	45	BE11523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-022	CTGCTGCTATTGCTCATG	130.84736
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11525_Aamu_Bax2006_COI	DRWA-024	1	BE11525_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	GCACAACCGGGATCTTTACTTC	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11525_Pcan_Bot2011_IGS	DRWA-024	1	BE11525_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-024	IGS	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	CGTAATCCTCAGTACTGGCTA	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11525_Pper_Dia2013_COI	DRWA-024	1	BE11525_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	CTTAGTGGCATTAATTCGDAATCC	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11525_Sspa_Oph2007_COI	DRWA-024	1	BE11525_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	GAAGGCCTTCCGATGATC	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11525_Upin_Bot2011_COI	DRWA-024	1	BE11525_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	CTTTAATTACAGCGTTTTTATTGTTGT	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11525_Vgib_Wil2025_COI	DRWA-024	1	BE11525_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	CCCCTCTTTACAAGTTCTTTAGGGT	130.85033
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11526_Asen_Wil2025_COI	DRWA-025	1	BE11526_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	GTTGTTGTTACAACACATGCTCTTTTG	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11526_Cjap_Sim2018_COI	DRWA-025	1	BE11526_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11526_Esin_And2018_Cyb	DRWA-025	1	BE11526_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-025	Cytb	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	ACCCCTCCTCATATCCAACCA	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11526_Mgig_Bot2012_COI	DRWA-025	1	BE11526_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	TCTTATTCGTTGGAGACTTTATAACCCT	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11526_Mjap_Gib2022_COI	DRWA-025	1	BE11526_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	AATTTAATTGTGACTAGGCATGGTCTG	130.84542
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11527_Cmae_Bot2011_COI	DRWA-026	1	BE11527_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	ATGAACAGTCTATCCTCCTTTAG	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11527_Hsan_Wil2021_COI	DRWA-026	1	BE11527_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	CCTGGGCCGGTATAGTAGGT	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11527_Mros_Gib2022_COI	DRWA-026	1	BE11527_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11527_Msal_Bot2012_COI	DRWA-026	1	BE11527_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	GYTAGTTCCRATGATGTTAGCTG	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11527_Mstr_Wil2021_COI	DRWA-026	1	BE11527_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	GTAGTGGTAACTACTCATGCTTTA	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11527_Pvir_Dia2013_COI	DRWA-026	1	BE11527_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	CTTAGTGGCATTAATTCGDAATCC	130.84675
BE11527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52021	WGS84	BE11527	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11527_Rhar_Sim2018_COI	DRWA-026	1	BE11527_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11527	Sample	BE11527	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-026	COI	45	BE11527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-026	CTGCTGCTATTGCTCATG	130.84675
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11529_Aamu_Bax2006_COI	DRWA-028	1	BE11529_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	GCACAACCGGGATCTTTACTTC	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11529_Pcan_Bot2011_IGS	DRWA-028	1	BE11529_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-028	IGS	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	CGTAATCCTCAGTACTGGCTA	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11529_Pper_Dia2013_COI	DRWA-028	1	BE11529_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	CTTAGTGGCATTAATTCGDAATCC	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11529_Sspa_Oph2007_COI	DRWA-028	1	BE11529_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	GAAGGCCTTCCGATGATC	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11529_Upin_Bot2011_COI	DRWA-028	1	BE11529_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	CTTTAATTACAGCGTTTTTATTGTTGT	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11529_Vgib_Wil2025_COI	DRWA-028	1	BE11529_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	CCCCTCTTTACAAGTTCTTTAGGGT	130.84944
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11530_Cmae_Bot2011_COI	DRWA-029	1	BE11530_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	ATGAACAGTCTATCCTCCTTTAG	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11530_Hsan_Wil2021_COI	DRWA-029	1	BE11530_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	CCTGGGCCGGTATAGTAGGT	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11530_Mros_Gib2022_COI	DRWA-029	1	BE11530_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11530_Msal_Bot2012_COI	DRWA-029	1	BE11530_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	GYTAGTTCCRATGATGTTAGCTG	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11530_Mstr_Wil2021_COI	DRWA-029	1	BE11530_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	GTAGTGGTAACTACTCATGCTTTA	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11530_Pvir_Dia2013_COI	DRWA-029	1	BE11530_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	CTTAGTGGCATTAATTCGDAATCC	130.84747
BE11530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51904	WGS84	BE11530	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11530_Rhar_Sim2018_COI	DRWA-029	1	BE11530_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11530	Sample	BE11530	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-029	COI	45	BE11530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-029	CTGCTGCTATTGCTCATG	130.84747
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11532_Aamu_Bax2006_COI	DRWA-031	1	BE11532_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	GCACAACCGGGATCTTTACTTC	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11532_Pcan_Bot2011_IGS	DRWA-031	1	BE11532_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-031	IGS	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	CGTAATCCTCAGTACTGGCTA	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11532_Pper_Dia2013_COI	DRWA-031	1	BE11532_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	CTTAGTGGCATTAATTCGDAATCC	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11532_Sspa_Oph2007_COI	DRWA-031	1	BE11532_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	GAAGGCCTTCCGATGATC	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11532_Upin_Bot2011_COI	DRWA-031	1	BE11532_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	CTTTAATTACAGCGTTTTTATTGTTGT	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11532_Vgib_Wil2025_COI	DRWA-031	1	BE11532_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	CCCCTCTTTACAAGTTCTTTAGGGT	130.85026
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11533_Asen_Wil2025_COI	DRWA-032	1	BE11533_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	GTTGTTGTTACAACACATGCTCTTTTG	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11533_Cjap_Sim2018_COI	DRWA-032	1	BE11533_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11533_Esin_And2018_Cyb	DRWA-032	1	BE11533_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-032	Cytb	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	ACCCCTCCTCATATCCAACCA	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11533_Mgig_Bot2012_COI	DRWA-032	1	BE11533_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	TCTTATTCGTTGGAGACTTTATAACCCT	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11533_Mjap_Gib2022_COI	DRWA-032	1	BE11533_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	AATTTAATTGTGACTAGGCATGGTCTG	130.85127
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11534_Cmae_Bot2011_COI	DRWA-033	1	BE11534_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	ATGAACAGTCTATCCTCCTTTAG	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11534_Hsan_Wil2021_COI	DRWA-033	1	BE11534_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	CCTGGGCCGGTATAGTAGGT	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11534_Mros_Gib2022_COI	DRWA-033	1	BE11534_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11534_Msal_Bot2012_COI	DRWA-033	1	BE11534_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	GYTAGTTCCRATGATGTTAGCTG	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11534_Mstr_Wil2021_COI	DRWA-033	1	BE11534_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	GTAGTGGTAACTACTCATGCTTTA	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11534_Pvir_Dia2013_COI	DRWA-033	1	BE11534_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	CTTAGTGGCATTAATTCGDAATCC	130.84738
BE11534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52359	WGS84	BE11534	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11534_Rhar_Sim2018_COI	DRWA-033	1	BE11534_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11534	Sample	BE11534	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-033	COI	45	BE11534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-033	CTGCTGCTATTGCTCATG	130.84738
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11536_Aamu_Bax2006_COI	DRWA-035	1	BE11536_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	GCACAACCGGGATCTTTACTTC	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11536_Pcan_Bot2011_IGS	DRWA-035	1	BE11536_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-035	IGS	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	CGTAATCCTCAGTACTGGCTA	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11536_Pper_Dia2013_COI	DRWA-035	1	BE11536_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	CTTAGTGGCATTAATTCGDAATCC	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11536_Sspa_Oph2007_COI	DRWA-035	1	BE11536_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	GAAGGCCTTCCGATGATC	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11536_Upin_Bot2011_COI	DRWA-035	1	BE11536_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	CTTTAATTACAGCGTTTTTATTGTTGT	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11536_Vgib_Wil2025_COI	DRWA-035	1	BE11536_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	CCCCTCTTTACAAGTTCTTTAGGGT	130.85165
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11541_Cmae_Bot2011_COI	DRWA-106	1	BE11541_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	ATGAACAGTCTATCCTCCTTTAG	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11541_Hsan_Wil2021_COI	DRWA-106	1	BE11541_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	CCTGGGCCGGTATAGTAGGT	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11541_Mros_Gib2022_COI	DRWA-106	1	BE11541_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11541_Msal_Bot2012_COI	DRWA-106	1	BE11541_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	GYTAGTTCCRATGATGTTAGCTG	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11541_Mstr_Wil2021_COI	DRWA-106	1	BE11541_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	GTAGTGGTAACTACTCATGCTTTA	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11541_Pvir_Dia2013_COI	DRWA-106	1	BE11541_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	CTTAGTGGCATTAATTCGDAATCC	130.90486
BE11541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51347	WGS84	BE11541	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11541_Rhar_Sim2018_COI	DRWA-106	1	BE11541_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11541	Sample	BE11541	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-106	COI	45	BE11541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-106	CTGCTGCTATTGCTCATG	130.90486
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11543_Aamu_Bax2006_COI	DRWA-108	1	BE11543_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	GCACAACCGGGATCTTTACTTC	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11543_Pcan_Bot2011_IGS	DRWA-108	1	BE11543_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-108	IGS	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	CGTAATCCTCAGTACTGGCTA	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11543_Pper_Dia2013_COI	DRWA-108	1	BE11543_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	CTTAGTGGCATTAATTCGDAATCC	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11543_Sspa_Oph2007_COI	DRWA-108	1	BE11543_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	GAAGGCCTTCCGATGATC	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11543_Upin_Bot2011_COI	DRWA-108	1	BE11543_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	CTTTAATTACAGCGTTTTTATTGTTGT	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11543_Vgib_Wil2025_COI	DRWA-108	1	BE11543_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	CCCCTCTTTACAAGTTCTTTAGGGT	130.90387
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11544_Asen_Wil2025_COI	DRWA-109	1	BE11544_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	GTTGTTGTTACAACACATGCTCTTTTG	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11544_Cjap_Sim2018_COI	DRWA-109	1	BE11544_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11544_Esin_And2018_Cyb	DRWA-109	1	BE11544_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-109	Cytb	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	ACCCCTCCTCATATCCAACCA	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11544_Mgig_Bot2012_COI	DRWA-109	1	BE11544_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	TCTTATTCGTTGGAGACTTTATAACCCT	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11544_Mjap_Gib2022_COI	DRWA-109	1	BE11544_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	AATTTAATTGTGACTAGGCATGGTCTG	130.90443
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11545_Cmae_Bot2011_COI	DRWA-110	1	BE11545_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	ATGAACAGTCTATCCTCCTTTAG	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11545_Hsan_Wil2021_COI	DRWA-110	1	BE11545_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	CCTGGGCCGGTATAGTAGGT	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11545_Mros_Gib2022_COI	DRWA-110	1	BE11545_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11545_Msal_Bot2012_COI	DRWA-110	1	BE11545_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	GYTAGTTCCRATGATGTTAGCTG	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11545_Mstr_Wil2021_COI	DRWA-110	1	BE11545_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	GTAGTGGTAACTACTCATGCTTTA	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11545_Pvir_Dia2013_COI	DRWA-110	1	BE11545_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	CTTAGTGGCATTAATTCGDAATCC	130.90553
BE11545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51039	WGS84	BE11545	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11545_Rhar_Sim2018_COI	DRWA-110	1	BE11545_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11545	Sample	BE11545	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-110	COI	45	BE11545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-110	CTGCTGCTATTGCTCATG	130.90553
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11547_Aamu_Bax2006_COI	DRWA-112	1	BE11547_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	GCACAACCGGGATCTTTACTTC	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11547_Pcan_Bot2011_IGS	DRWA-112	1	BE11547_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-112	IGS	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	CGTAATCCTCAGTACTGGCTA	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11547_Pper_Dia2013_COI	DRWA-112	1	BE11547_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	CTTAGTGGCATTAATTCGDAATCC	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11547_Sspa_Oph2007_COI	DRWA-112	1	BE11547_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	GAAGGCCTTCCGATGATC	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11547_Upin_Bot2011_COI	DRWA-112	1	BE11547_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	CTTTAATTACAGCGTTTTTATTGTTGT	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11547_Vgib_Wil2025_COI	DRWA-112	1	BE11547_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	CCCCTCTTTACAAGTTCTTTAGGGT	130.90503
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11548_Asen_Wil2025_COI	DRWA-113	1	BE11548_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	GTTGTTGTTACAACACATGCTCTTTTG	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11548_Cjap_Sim2018_COI	DRWA-113	1	BE11548_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11548_Esin_And2018_Cyb	DRWA-113	1	BE11548_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-113	Cytb	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	ACCCCTCCTCATATCCAACCA	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11548_Mgig_Bot2012_COI	DRWA-113	1	BE11548_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	TCTTATTCGTTGGAGACTTTATAACCCT	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11548_Mjap_Gib2022_COI	DRWA-113	1	BE11548_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	AATTTAATTGTGACTAGGCATGGTCTG	130.90438
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11549_Cmae_Bot2011_COI	DRWA-114	1	BE11549_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	ATGAACAGTCTATCCTCCTTTAG	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11549_Hsan_Wil2021_COI	DRWA-114	1	BE11549_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	CCTGGGCCGGTATAGTAGGT	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11549_Mros_Gib2022_COI	DRWA-114	1	BE11549_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11549_Msal_Bot2012_COI	DRWA-114	1	BE11549_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	GYTAGTTCCRATGATGTTAGCTG	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11549_Mstr_Wil2021_COI	DRWA-114	1	BE11549_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	GTAGTGGTAACTACTCATGCTTTA	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11549_Pvir_Dia2013_COI	DRWA-114	1	BE11549_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	CTTAGTGGCATTAATTCGDAATCC	130.90575
BE11549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50838	WGS84	BE11549	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11549_Rhar_Sim2018_COI	DRWA-114	1	BE11549_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11549	Sample	BE11549	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-114	COI	45	BE11549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-114	CTGCTGCTATTGCTCATG	130.90575
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11550_Aamu_Bax2006_COI	DRWA-115	1	BE11550_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	GCACAACCGGGATCTTTACTTC	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11550_Pcan_Bot2011_IGS	DRWA-115	1	BE11550_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-115	IGS	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	CGTAATCCTCAGTACTGGCTA	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11550_Pper_Dia2013_COI	DRWA-115	1	BE11550_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	CTTAGTGGCATTAATTCGDAATCC	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11550_Sspa_Oph2007_COI	DRWA-115	1	BE11550_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	GAAGGCCTTCCGATGATC	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11550_Upin_Bot2011_COI	DRWA-115	1	BE11550_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	CTTTAATTACAGCGTTTTTATTGTTGT	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11550_Vgib_Wil2025_COI	DRWA-115	1	BE11550_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	CCCCTCTTTACAAGTTCTTTAGGGT	130.90702
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11551_Asen_Wil2025_COI	DRWA-116	1	BE11551_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	GTTGTTGTTACAACACATGCTCTTTTG	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11551_Cjap_Sim2018_COI	DRWA-116	1	BE11551_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11551_Esin_And2018_Cyb	DRWA-116	1	BE11551_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-116	Cytb	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	ACCCCTCCTCATATCCAACCA	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11551_Mgig_Bot2012_COI	DRWA-116	1	BE11551_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	TCTTATTCGTTGGAGACTTTATAACCCT	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11551_Mjap_Gib2022_COI	DRWA-116	1	BE11551_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	AATTTAATTGTGACTAGGCATGGTCTG	130.90822
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11552_Cmae_Bot2011_COI	DRWA-117	1	BE11552_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	ATGAACAGTCTATCCTCCTTTAG	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11552_Hsan_Wil2021_COI	DRWA-117	1	BE11552_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	CCTGGGCCGGTATAGTAGGT	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11552_Mros_Gib2022_COI	DRWA-117	1	BE11552_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11552_Msal_Bot2012_COI	DRWA-117	1	BE11552_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	GYTAGTTCCRATGATGTTAGCTG	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11552_Mstr_Wil2021_COI	DRWA-117	1	BE11552_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	GTAGTGGTAACTACTCATGCTTTA	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11552_Pvir_Dia2013_COI	DRWA-117	1	BE11552_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	CTTAGTGGCATTAATTCGDAATCC	130.90413
BE11552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50732	WGS84	BE11552	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11552_Rhar_Sim2018_COI	DRWA-117	1	BE11552_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11552	Sample	BE11552	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-117	COI	45	BE11552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-117	CTGCTGCTATTGCTCATG	130.90413
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11554_Aamu_Bax2006_COI	DRWA-119	1	BE11554_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	GCACAACCGGGATCTTTACTTC	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11554_Pcan_Bot2011_IGS	DRWA-119	1	BE11554_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-119	IGS	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	CGTAATCCTCAGTACTGGCTA	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11554_Pper_Dia2013_COI	DRWA-119	1	BE11554_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	CTTAGTGGCATTAATTCGDAATCC	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11554_Sspa_Oph2007_COI	DRWA-119	1	BE11554_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	GAAGGCCTTCCGATGATC	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11554_Upin_Bot2011_COI	DRWA-119	1	BE11554_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	CTTTAATTACAGCGTTTTTATTGTTGT	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11554_Vgib_Wil2025_COI	DRWA-119	1	BE11554_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	CCCCTCTTTACAAGTTCTTTAGGGT	130.90649
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11555_Asen_Wil2025_COI	DRWA-120	1	BE11555_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	GTTGTTGTTACAACACATGCTCTTTTG	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11555_Cjap_Sim2018_COI	DRWA-120	1	BE11555_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11555_Esin_And2018_Cyb	DRWA-120	1	BE11555_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-120	Cytb	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	ACCCCTCCTCATATCCAACCA	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11555_Mgig_Bot2012_COI	DRWA-120	1	BE11555_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	TCTTATTCGTTGGAGACTTTATAACCCT	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11555_Mjap_Gib2022_COI	DRWA-120	1	BE11555_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	AATTTAATTGTGACTAGGCATGGTCTG	130.90767
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11556_Cmae_Bot2011_COI	DRWA-121	1	BE11556_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	ATGAACAGTCTATCCTCCTTTAG	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11556_Hsan_Wil2021_COI	DRWA-121	1	BE11556_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	CCTGGGCCGGTATAGTAGGT	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11556_Mros_Gib2022_COI	DRWA-121	1	BE11556_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11556_Msal_Bot2012_COI	DRWA-121	1	BE11556_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	GYTAGTTCCRATGATGTTAGCTG	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11556_Mstr_Wil2021_COI	DRWA-121	1	BE11556_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	GTAGTGGTAACTACTCATGCTTTA	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11556_Pvir_Dia2013_COI	DRWA-121	1	BE11556_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	CTTAGTGGCATTAATTCGDAATCC	130.90885
BE11556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50742	WGS84	BE11556	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11556_Rhar_Sim2018_COI	DRWA-121	1	BE11556_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11556	Sample	BE11556	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-121	COI	45	BE11556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-121	CTGCTGCTATTGCTCATG	130.90885
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11558_Aamu_Bax2006_COI	DRWA-123	1	BE11558_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	GCACAACCGGGATCTTTACTTC	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11558_Pcan_Bot2011_IGS	DRWA-123	1	BE11558_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-123	IGS	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	CGTAATCCTCAGTACTGGCTA	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11558_Pper_Dia2013_COI	DRWA-123	1	BE11558_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	CTTAGTGGCATTAATTCGDAATCC	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11558_Sspa_Oph2007_COI	DRWA-123	1	BE11558_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	GAAGGCCTTCCGATGATC	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11558_Upin_Bot2011_COI	DRWA-123	1	BE11558_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	CTTTAATTACAGCGTTTTTATTGTTGT	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11558_Vgib_Wil2025_COI	DRWA-123	1	BE11558_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	CCCCTCTTTACAAGTTCTTTAGGGT	130.9111
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11559_Asen_Wil2025_COI	DRWA-124	1	BE11559_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	GTTGTTGTTACAACACATGCTCTTTTG	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11559_Cjap_Sim2018_COI	DRWA-124	1	BE11559_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11559_Esin_And2018_Cyb	DRWA-124	1	BE11559_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-124	Cytb	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	ACCCCTCCTCATATCCAACCA	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11559_Mgig_Bot2012_COI	DRWA-124	1	BE11559_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	TCTTATTCGTTGGAGACTTTATAACCCT	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11559_Mjap_Gib2022_COI	DRWA-124	1	BE11559_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	AATTTAATTGTGACTAGGCATGGTCTG	130.91247
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11561_Aamu_Bax2006_COI	DRWA-126	1	BE11561_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	GCACAACCGGGATCTTTACTTC	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11561_Pcan_Bot2011_IGS	DRWA-126	1	BE11561_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-126	IGS	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	CGTAATCCTCAGTACTGGCTA	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11561_Pper_Dia2013_COI	DRWA-126	1	BE11561_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	CTTAGTGGCATTAATTCGDAATCC	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11561_Sspa_Oph2007_COI	DRWA-126	1	BE11561_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	GAAGGCCTTCCGATGATC	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11561_Upin_Bot2011_COI	DRWA-126	1	BE11561_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	CTTTAATTACAGCGTTTTTATTGTTGT	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11561_Vgib_Wil2025_COI	DRWA-126	1	BE11561_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	CCCCTCTTTACAAGTTCTTTAGGGT	130.90563
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11562_Asen_Wil2025_COI	DRWA-127	1	BE11562_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	GTTGTTGTTACAACACATGCTCTTTTG	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11562_Cjap_Sim2018_COI	DRWA-127	1	BE11562_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11562_Esin_And2018_Cyb	DRWA-127	1	BE11562_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-127	Cytb	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	ACCCCTCCTCATATCCAACCA	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11562_Mgig_Bot2012_COI	DRWA-127	1	BE11562_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	TCTTATTCGTTGGAGACTTTATAACCCT	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11562_Mjap_Gib2022_COI	DRWA-127	1	BE11562_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	AATTTAATTGTGACTAGGCATGGTCTG	130.90671
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11563_Cmae_Bot2011_COI	DRWA-128	1	BE11563_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	ATGAACAGTCTATCCTCCTTTAG	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11563_Hsan_Wil2021_COI	DRWA-128	1	BE11563_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	CCTGGGCCGGTATAGTAGGT	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11563_Mros_Gib2022_COI	DRWA-128	1	BE11563_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11563_Msal_Bot2012_COI	DRWA-128	1	BE11563_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	GYTAGTTCCRATGATGTTAGCTG	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11563_Mstr_Wil2021_COI	DRWA-128	1	BE11563_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	GTAGTGGTAACTACTCATGCTTTA	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11563_Pvir_Dia2013_COI	DRWA-128	1	BE11563_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	CTTAGTGGCATTAATTCGDAATCC	130.90833
BE11563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11563	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11563_Rhar_Sim2018_COI	DRWA-128	1	BE11563_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11563	Sample	BE11563	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-128	COI	45	BE11563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-128	CTGCTGCTATTGCTCATG	130.90833
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11565_Aamu_Bax2006_COI	DRWA-130	1	BE11565_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	GCACAACCGGGATCTTTACTTC	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11565_Pcan_Bot2011_IGS	DRWA-130	1	BE11565_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-130	IGS	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	CGTAATCCTCAGTACTGGCTA	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11565_Pper_Dia2013_COI	DRWA-130	1	BE11565_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	CTTAGTGGCATTAATTCGDAATCC	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11565_Sspa_Oph2007_COI	DRWA-130	1	BE11565_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	GAAGGCCTTCCGATGATC	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11565_Upin_Bot2011_COI	DRWA-130	1	BE11565_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	CTTTAATTACAGCGTTTTTATTGTTGT	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11565_Vgib_Wil2025_COI	DRWA-130	1	BE11565_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	CCCCTCTTTACAAGTTCTTTAGGGT	130.91053
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11566_Asen_Wil2025_COI	DRWA-131	1	BE11566_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	GTTGTTGTTACAACACATGCTCTTTTG	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11566_Cjap_Sim2018_COI	DRWA-131	1	BE11566_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11566_Esin_And2018_Cyb	DRWA-131	1	BE11566_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-131	Cytb	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	ACCCCTCCTCATATCCAACCA	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11566_Mgig_Bot2012_COI	DRWA-131	1	BE11566_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	TCTTATTCGTTGGAGACTTTATAACCCT	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11566_Mjap_Gib2022_COI	DRWA-131	1	BE11566_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	AATTTAATTGTGACTAGGCATGGTCTG	130.91196
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11567_Cmae_Bot2011_COI	DRWA-132	1	BE11567_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	ATGAACAGTCTATCCTCCTTTAG	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11567_Hsan_Wil2021_COI	DRWA-132	1	BE11567_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	CCTGGGCCGGTATAGTAGGT	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11567_Mros_Gib2022_COI	DRWA-132	1	BE11567_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11567_Msal_Bot2012_COI	DRWA-132	1	BE11567_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	GYTAGTTCCRATGATGTTAGCTG	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11567_Mstr_Wil2021_COI	DRWA-132	1	BE11567_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	GTAGTGGTAACTACTCATGCTTTA	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11567_Pvir_Dia2013_COI	DRWA-132	1	BE11567_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	CTTAGTGGCATTAATTCGDAATCC	130.91302
BE11567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50659	WGS84	BE11567	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11567_Rhar_Sim2018_COI	DRWA-132	1	BE11567_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11567	Sample	BE11567	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-132	COI	45	BE11567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-132	CTGCTGCTATTGCTCATG	130.91302
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11569_Aamu_Bax2006_COI	DRWA-134	1	BE11569_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	GCACAACCGGGATCTTTACTTC	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11569_Pcan_Bot2011_IGS	DRWA-134	1	BE11569_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-134	IGS	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	CGTAATCCTCAGTACTGGCTA	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11569_Pper_Dia2013_COI	DRWA-134	1	BE11569_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	CTTAGTGGCATTAATTCGDAATCC	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11569_Sspa_Oph2007_COI	DRWA-134	1	BE11569_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	GAAGGCCTTCCGATGATC	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11569_Upin_Bot2011_COI	DRWA-134	1	BE11569_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	CTTTAATTACAGCGTTTTTATTGTTGT	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11569_Vgib_Wil2025_COI	DRWA-134	1	BE11569_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	CCCCTCTTTACAAGTTCTTTAGGGT	130.91543
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11570_Cmae_Bot2011_COI	DRWA-135	1	BE11570_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	ATGAACAGTCTATCCTCCTTTAG	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11570_Hsan_Wil2021_COI	DRWA-135	1	BE11570_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	CCTGGGCCGGTATAGTAGGT	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11570_Mros_Gib2022_COI	DRWA-135	1	BE11570_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11570_Msal_Bot2012_COI	DRWA-135	1	BE11570_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	GYTAGTTCCRATGATGTTAGCTG	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11570_Mstr_Wil2021_COI	DRWA-135	1	BE11570_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	GTAGTGGTAACTACTCATGCTTTA	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11570_Pvir_Dia2013_COI	DRWA-135	1	BE11570_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	CTTAGTGGCATTAATTCGDAATCC	130.90891
BE11570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50547	WGS84	BE11570	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11570_Rhar_Sim2018_COI	DRWA-135	1	BE11570_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11570	Sample	BE11570	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-135	COI	45	BE11570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-135	CTGCTGCTATTGCTCATG	130.90891
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11572_Aamu_Bax2006_COI	DRWA-137	1	BE11572_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	GCACAACCGGGATCTTTACTTC	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11572_Pcan_Bot2011_IGS	DRWA-137	1	BE11572_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-137	IGS	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	CGTAATCCTCAGTACTGGCTA	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11572_Pper_Dia2013_COI	DRWA-137	1	BE11572_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	CTTAGTGGCATTAATTCGDAATCC	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11572_Sspa_Oph2007_COI	DRWA-137	1	BE11572_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	GAAGGCCTTCCGATGATC	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11572_Upin_Bot2011_COI	DRWA-137	1	BE11572_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	CTTTAATTACAGCGTTTTTATTGTTGT	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11572_Vgib_Wil2025_COI	DRWA-137	1	BE11572_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	CCCCTCTTTACAAGTTCTTTAGGGT	130.91144
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11573_Asen_Wil2025_COI	DRWA-138	1	BE11573_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	GTTGTTGTTACAACACATGCTCTTTTG	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11573_Cjap_Sim2018_COI	DRWA-138	1	BE11573_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11573_Esin_And2018_Cyb	DRWA-138	1	BE11573_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-138	Cytb	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	ACCCCTCCTCATATCCAACCA	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11573_Mgig_Bot2012_COI	DRWA-138	1	BE11573_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	TCTTATTCGTTGGAGACTTTATAACCCT	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11573_Mjap_Gib2022_COI	DRWA-138	1	BE11573_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	AATTTAATTGTGACTAGGCATGGTCTG	130.91245
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11574_Cmae_Bot2011_COI	DRWA-139	1	BE11574_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	ATGAACAGTCTATCCTCCTTTAG	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11574_Hsan_Wil2021_COI	DRWA-139	1	BE11574_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	CCTGGGCCGGTATAGTAGGT	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11574_Mros_Gib2022_COI	DRWA-139	1	BE11574_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11574_Msal_Bot2012_COI	DRWA-139	1	BE11574_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	GYTAGTTCCRATGATGTTAGCTG	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11574_Mstr_Wil2021_COI	DRWA-139	1	BE11574_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	GTAGTGGTAACTACTCATGCTTTA	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11574_Pvir_Dia2013_COI	DRWA-139	1	BE11574_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	CTTAGTGGCATTAATTCGDAATCC	130.91373
BE11574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50549	WGS84	BE11574	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11574_Rhar_Sim2018_COI	DRWA-139	1	BE11574_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11574	Sample	BE11574	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-139	COI	45	BE11574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-139	CTGCTGCTATTGCTCATG	130.91373
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11580_Asen_Wil2025_COI	DRWA-036	1	BE11580_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	GTTGTTGTTACAACACATGCTCTTTTG	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11580_Cjap_Sim2018_COI	DRWA-036	1	BE11580_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11580_Esin_And2018_Cyb	DRWA-036	1	BE11580_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-036	Cytb	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	ACCCCTCCTCATATCCAACCA	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11580_Mgig_Bot2012_COI	DRWA-036	1	BE11580_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	TCTTATTCGTTGGAGACTTTATAACCCT	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11580_Mjap_Gib2022_COI	DRWA-036	1	BE11580_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	AATTTAATTGTGACTAGGCATGGTCTG	130.93098
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11581_Cmae_Bot2011_COI	DRWA-037	1	BE11581_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	ATGAACAGTCTATCCTCCTTTAG	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11581_Hsan_Wil2021_COI	DRWA-037	1	BE11581_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	CCTGGGCCGGTATAGTAGGT	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11581_Mros_Gib2022_COI	DRWA-037	1	BE11581_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11581_Msal_Bot2012_COI	DRWA-037	1	BE11581_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	GYTAGTTCCRATGATGTTAGCTG	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11581_Mstr_Wil2021_COI	DRWA-037	1	BE11581_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	GTAGTGGTAACTACTCATGCTTTA	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11581_Pvir_Dia2013_COI	DRWA-037	1	BE11581_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	CTTAGTGGCATTAATTCGDAATCC	130.92944
BE11581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11581	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11581_Rhar_Sim2018_COI	DRWA-037	1	BE11581_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11581	Sample	BE11581	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-037	COI	45	BE11581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-037	CTGCTGCTATTGCTCATG	130.92944
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11583_Aamu_Bax2006_COI	DRWA-039	1	BE11583_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	GCACAACCGGGATCTTTACTTC	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11583_Pcan_Bot2011_IGS	DRWA-039	1	BE11583_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-039	IGS	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	CGTAATCCTCAGTACTGGCTA	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11583_Pper_Dia2013_COI	DRWA-039	1	BE11583_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	CTTAGTGGCATTAATTCGDAATCC	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11583_Sspa_Oph2007_COI	DRWA-039	1	BE11583_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	GAAGGCCTTCCGATGATC	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11583_Upin_Bot2011_COI	DRWA-039	1	BE11583_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	CTTTAATTACAGCGTTTTTATTGTTGT	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11583_Vgib_Wil2025_COI	DRWA-039	1	BE11583_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	CCCCTCTTTACAAGTTCTTTAGGGT	130.93139
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11584_Asen_Wil2025_COI	DRWA-040	1	BE11584_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	GTTGTTGTTACAACACATGCTCTTTTG	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11584_Cjap_Sim2018_COI	DRWA-040	1	BE11584_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11584_Esin_And2018_Cyb	DRWA-040	1	BE11584_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-040	Cytb	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	ACCCCTCCTCATATCCAACCA	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11584_Mgig_Bot2012_COI	DRWA-040	1	BE11584_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	TCTTATTCGTTGGAGACTTTATAACCCT	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11584_Mjap_Gib2022_COI	DRWA-040	1	BE11584_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	AATTTAATTGTGACTAGGCATGGTCTG	130.92998
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11585_Cmae_Bot2011_COI	DRWA-041	1	BE11585_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	ATGAACAGTCTATCCTCCTTTAG	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11585_Hsan_Wil2021_COI	DRWA-041	1	BE11585_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	CCTGGGCCGGTATAGTAGGT	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11585_Mros_Gib2022_COI	DRWA-041	1	BE11585_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11585_Msal_Bot2012_COI	DRWA-041	1	BE11585_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	GYTAGTTCCRATGATGTTAGCTG	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11585_Mstr_Wil2021_COI	DRWA-041	1	BE11585_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	GTAGTGGTAACTACTCATGCTTTA	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11585_Pvir_Dia2013_COI	DRWA-041	1	BE11585_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	CTTAGTGGCATTAATTCGDAATCC	130.93095
BE11585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51358	WGS84	BE11585	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11585_Rhar_Sim2018_COI	DRWA-041	1	BE11585_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11585	Sample	BE11585	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-041	COI	45	BE11585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-041	CTGCTGCTATTGCTCATG	130.93095
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11587_Aamu_Bax2006_COI	DRWA-043	1	BE11587_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	GCACAACCGGGATCTTTACTTC	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11587_Pcan_Bot2011_IGS	DRWA-043	1	BE11587_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-043	IGS	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	CGTAATCCTCAGTACTGGCTA	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11587_Pper_Dia2013_COI	DRWA-043	1	BE11587_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	CTTAGTGGCATTAATTCGDAATCC	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11587_Sspa_Oph2007_COI	DRWA-043	1	BE11587_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	GAAGGCCTTCCGATGATC	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11587_Upin_Bot2011_COI	DRWA-043	1	BE11587_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	CTTTAATTACAGCGTTTTTATTGTTGT	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11587_Vgib_Wil2025_COI	DRWA-043	1	BE11587_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	CCCCTCTTTACAAGTTCTTTAGGGT	130.9301
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11588_Asen_Wil2025_COI	DRWA-044	1	BE11588_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	GTTGTTGTTACAACACATGCTCTTTTG	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11588_Cjap_Sim2018_COI	DRWA-044	1	BE11588_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11588_Esin_And2018_Cyb	DRWA-044	1	BE11588_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-044	Cytb	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	ACCCCTCCTCATATCCAACCA	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11588_Mgig_Bot2012_COI	DRWA-044	1	BE11588_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	TCTTATTCGTTGGAGACTTTATAACCCT	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11588_Mjap_Gib2022_COI	DRWA-044	1	BE11588_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	AATTTAATTGTGACTAGGCATGGTCTG	130.93111
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11589_Cmae_Bot2011_COI	DRWA-045	1	BE11589_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	ATGAACAGTCTATCCTCCTTTAG	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11589_Hsan_Wil2021_COI	DRWA-045	1	BE11589_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	CCTGGGCCGGTATAGTAGGT	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11589_Mros_Gib2022_COI	DRWA-045	1	BE11589_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11589_Msal_Bot2012_COI	DRWA-045	1	BE11589_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	GYTAGTTCCRATGATGTTAGCTG	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11589_Mstr_Wil2021_COI	DRWA-045	1	BE11589_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	GTAGTGGTAACTACTCATGCTTTA	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11589_Pvir_Dia2013_COI	DRWA-045	1	BE11589_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	CTTAGTGGCATTAATTCGDAATCC	130.9322
BE11589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5131	WGS84	BE11589	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11589_Rhar_Sim2018_COI	DRWA-045	1	BE11589_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11589	Sample	BE11589	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-045	COI	45	BE11589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-045	CTGCTGCTATTGCTCATG	130.9322
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11590_Aamu_Bax2006_COI	DRWA-046	1	BE11590_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	GCACAACCGGGATCTTTACTTC	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11590_Pcan_Bot2011_IGS	DRWA-046	1	BE11590_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-046	IGS	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	CGTAATCCTCAGTACTGGCTA	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11590_Pper_Dia2013_COI	DRWA-046	1	BE11590_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	CTTAGTGGCATTAATTCGDAATCC	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11590_Sspa_Oph2007_COI	DRWA-046	1	BE11590_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	GAAGGCCTTCCGATGATC	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11590_Upin_Bot2011_COI	DRWA-046	1	BE11590_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	CTTTAATTACAGCGTTTTTATTGTTGT	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11590_Vgib_Wil2025_COI	DRWA-046	1	BE11590_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	CCCCTCTTTACAAGTTCTTTAGGGT	130.92965
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11591_Asen_Wil2025_COI	DRWA-047	1	BE11591_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	GTTGTTGTTACAACACATGCTCTTTTG	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11591_Cjap_Sim2018_COI	DRWA-047	1	BE11591_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11591_Esin_And2018_Cyb	DRWA-047	1	BE11591_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-047	Cytb	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	ACCCCTCCTCATATCCAACCA	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11591_Mgig_Bot2012_COI	DRWA-047	1	BE11591_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	TCTTATTCGTTGGAGACTTTATAACCCT	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11591_Mjap_Gib2022_COI	DRWA-047	1	BE11591_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	AATTTAATTGTGACTAGGCATGGTCTG	130.93074
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11592_Cmae_Bot2011_COI	DRWA-048	1	BE11592_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	ATGAACAGTCTATCCTCCTTTAG	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11592_Hsan_Wil2021_COI	DRWA-048	1	BE11592_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	CCTGGGCCGGTATAGTAGGT	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11592_Mros_Gib2022_COI	DRWA-048	1	BE11592_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11592_Msal_Bot2012_COI	DRWA-048	1	BE11592_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	GYTAGTTCCRATGATGTTAGCTG	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11592_Mstr_Wil2021_COI	DRWA-048	1	BE11592_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	GTAGTGGTAACTACTCATGCTTTA	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11592_Pvir_Dia2013_COI	DRWA-048	1	BE11592_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	CTTAGTGGCATTAATTCGDAATCC	130.9317
BE11592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51229	WGS84	BE11592	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11592_Rhar_Sim2018_COI	DRWA-048	1	BE11592_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11592	Sample	BE11592	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-048	COI	45	BE11592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-048	CTGCTGCTATTGCTCATG	130.9317
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11594_Aamu_Bax2006_COI	DRWA-050	1	BE11594_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	GCACAACCGGGATCTTTACTTC	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11594_Pcan_Bot2011_IGS	DRWA-050	1	BE11594_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-050	IGS	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	CGTAATCCTCAGTACTGGCTA	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11594_Pper_Dia2013_COI	DRWA-050	1	BE11594_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	CTTAGTGGCATTAATTCGDAATCC	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11594_Sspa_Oph2007_COI	DRWA-050	1	BE11594_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	GAAGGCCTTCCGATGATC	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11594_Upin_Bot2011_COI	DRWA-050	1	BE11594_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	CTTTAATTACAGCGTTTTTATTGTTGT	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11594_Vgib_Wil2025_COI	DRWA-050	1	BE11594_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	CCCCTCTTTACAAGTTCTTTAGGGT	130.93018
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11595_Asen_Wil2025_COI	DRWA-051	1	BE11595_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	GTTGTTGTTACAACACATGCTCTTTTG	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11595_Cjap_Sim2018_COI	DRWA-051	1	BE11595_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11595_Esin_And2018_Cyb	DRWA-051	1	BE11595_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-051	Cytb	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	ACCCCTCCTCATATCCAACCA	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11595_Mgig_Bot2012_COI	DRWA-051	1	BE11595_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	TCTTATTCGTTGGAGACTTTATAACCCT	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11595_Mjap_Gib2022_COI	DRWA-051	1	BE11595_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	AATTTAATTGTGACTAGGCATGGTCTG	130.93119
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11596_Cmae_Bot2011_COI	DRWA-052	1	BE11596_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	ATGAACAGTCTATCCTCCTTTAG	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11596_Hsan_Wil2021_COI	DRWA-052	1	BE11596_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	CCTGGGCCGGTATAGTAGGT	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11596_Mros_Gib2022_COI	DRWA-052	1	BE11596_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11596_Msal_Bot2012_COI	DRWA-052	1	BE11596_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	GYTAGTTCCRATGATGTTAGCTG	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11596_Mstr_Wil2021_COI	DRWA-052	1	BE11596_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	GTAGTGGTAACTACTCATGCTTTA	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11596_Pvir_Dia2013_COI	DRWA-052	1	BE11596_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	CTTAGTGGCATTAATTCGDAATCC	130.9322
BE11596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51117	WGS84	BE11596	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11596_Rhar_Sim2018_COI	DRWA-052	1	BE11596_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11596	Sample	BE11596	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-052	COI	45	BE11596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-052	CTGCTGCTATTGCTCATG	130.9322
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11598_Aamu_Bax2006_COI	DRWA-054	1	BE11598_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	GCACAACCGGGATCTTTACTTC	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11598_Pcan_Bot2011_IGS	DRWA-054	1	BE11598_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-054	IGS	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	CGTAATCCTCAGTACTGGCTA	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11598_Pper_Dia2013_COI	DRWA-054	1	BE11598_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	CTTAGTGGCATTAATTCGDAATCC	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11598_Sspa_Oph2007_COI	DRWA-054	1	BE11598_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	GAAGGCCTTCCGATGATC	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11598_Upin_Bot2011_COI	DRWA-054	1	BE11598_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	CTTTAATTACAGCGTTTTTATTGTTGT	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11598_Vgib_Wil2025_COI	DRWA-054	1	BE11598_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	CCCCTCTTTACAAGTTCTTTAGGGT	130.92966
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11599_Asen_Wil2025_COI	DRWA-055	1	BE11599_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	GTTGTTGTTACAACACATGCTCTTTTG	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11599_Cjap_Sim2018_COI	DRWA-055	1	BE11599_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11599_Esin_And2018_Cyb	DRWA-055	1	BE11599_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-055	Cytb	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	ACCCCTCCTCATATCCAACCA	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11599_Mgig_Bot2012_COI	DRWA-055	1	BE11599_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	TCTTATTCGTTGGAGACTTTATAACCCT	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11599_Mjap_Gib2022_COI	DRWA-055	1	BE11599_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	AATTTAATTGTGACTAGGCATGGTCTG	130.93069
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11600_Cmae_Bot2011_COI	DRWA-056	1	BE11600_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	ATGAACAGTCTATCCTCCTTTAG	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11600_Hsan_Wil2021_COI	DRWA-056	1	BE11600_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	CCTGGGCCGGTATAGTAGGT	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11600_Mros_Gib2022_COI	DRWA-056	1	BE11600_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11600_Msal_Bot2012_COI	DRWA-056	1	BE11600_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	GYTAGTTCCRATGATGTTAGCTG	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11600_Mstr_Wil2021_COI	DRWA-056	1	BE11600_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	GTAGTGGTAACTACTCATGCTTTA	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11600_Pvir_Dia2013_COI	DRWA-056	1	BE11600_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	CTTAGTGGCATTAATTCGDAATCC	130.93181
BE11600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51061	WGS84	BE11600	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11600_Rhar_Sim2018_COI	DRWA-056	1	BE11600_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11600	Sample	BE11600	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-056	COI	45	BE11600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-056	CTGCTGCTATTGCTCATG	130.93181
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11602_Aamu_Bax2006_COI	DRWA-058	1	BE11602_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	GCACAACCGGGATCTTTACTTC	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11602_Pcan_Bot2011_IGS	DRWA-058	1	BE11602_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-058	IGS	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	CGTAATCCTCAGTACTGGCTA	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11602_Pper_Dia2013_COI	DRWA-058	1	BE11602_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	CTTAGTGGCATTAATTCGDAATCC	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11602_Sspa_Oph2007_COI	DRWA-058	1	BE11602_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	GAAGGCCTTCCGATGATC	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11602_Upin_Bot2011_COI	DRWA-058	1	BE11602_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	CTTTAATTACAGCGTTTTTATTGTTGT	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11602_Vgib_Wil2025_COI	DRWA-058	1	BE11602_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	CCCCTCTTTACAAGTTCTTTAGGGT	130.92919
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11603_Asen_Wil2025_COI	DRWA-059	1	BE11603_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	GTTGTTGTTACAACACATGCTCTTTTG	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11603_Cjap_Sim2018_COI	DRWA-059	1	BE11603_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11603_Esin_And2018_Cyb	DRWA-059	1	BE11603_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-059	Cytb	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	ACCCCTCCTCATATCCAACCA	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11603_Mgig_Bot2012_COI	DRWA-059	1	BE11603_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	TCTTATTCGTTGGAGACTTTATAACCCT	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11603_Mjap_Gib2022_COI	DRWA-059	1	BE11603_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	AATTTAATTGTGACTAGGCATGGTCTG	130.93037
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11604_Cmae_Bot2011_COI	DRWA-060	1	BE11604_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	ATGAACAGTCTATCCTCCTTTAG	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11604_Hsan_Wil2021_COI	DRWA-060	1	BE11604_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	CCTGGGCCGGTATAGTAGGT	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11604_Mros_Gib2022_COI	DRWA-060	1	BE11604_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11604_Msal_Bot2012_COI	DRWA-060	1	BE11604_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	GYTAGTTCCRATGATGTTAGCTG	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11604_Mstr_Wil2021_COI	DRWA-060	1	BE11604_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	GTAGTGGTAACTACTCATGCTTTA	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11604_Pvir_Dia2013_COI	DRWA-060	1	BE11604_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	CTTAGTGGCATTAATTCGDAATCC	130.93128
BE11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50967	WGS84	BE11604	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11604_Rhar_Sim2018_COI	DRWA-060	1	BE11604_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11604	Sample	BE11604	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-060	COI	45	BE11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-060	CTGCTGCTATTGCTCATG	130.93128
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11606_Aamu_Bax2006_COI	DRWA-062	1	BE11606_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	GCACAACCGGGATCTTTACTTC	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11606_Pcan_Bot2011_IGS	DRWA-062	1	BE11606_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-062	IGS	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	CGTAATCCTCAGTACTGGCTA	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11606_Pper_Dia2013_COI	DRWA-062	1	BE11606_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	CTTAGTGGCATTAATTCGDAATCC	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11606_Sspa_Oph2007_COI	DRWA-062	1	BE11606_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	GAAGGCCTTCCGATGATC	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11606_Upin_Bot2011_COI	DRWA-062	1	BE11606_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	CTTTAATTACAGCGTTTTTATTGTTGT	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11606_Vgib_Wil2025_COI	DRWA-062	1	BE11606_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	CCCCTCTTTACAAGTTCTTTAGGGT	130.92868
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11607_Asen_Wil2025_COI	DRWA-063	1	BE11607_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	GTTGTTGTTACAACACATGCTCTTTTG	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11607_Cjap_Sim2018_COI	DRWA-063	1	BE11607_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11607_Esin_And2018_Cyb	DRWA-063	1	BE11607_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-063	Cytb	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	ACCCCTCCTCATATCCAACCA	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11607_Mgig_Bot2012_COI	DRWA-063	1	BE11607_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	TCTTATTCGTTGGAGACTTTATAACCCT	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11607_Mjap_Gib2022_COI	DRWA-063	1	BE11607_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	AATTTAATTGTGACTAGGCATGGTCTG	130.92967
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11608_Cmae_Bot2011_COI	DRWA-064	1	BE11608_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	ATGAACAGTCTATCCTCCTTTAG	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11608_Hsan_Wil2021_COI	DRWA-064	1	BE11608_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	CCTGGGCCGGTATAGTAGGT	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11608_Mros_Gib2022_COI	DRWA-064	1	BE11608_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11608_Msal_Bot2012_COI	DRWA-064	1	BE11608_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	GYTAGTTCCRATGATGTTAGCTG	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11608_Mstr_Wil2021_COI	DRWA-064	1	BE11608_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	GTAGTGGTAACTACTCATGCTTTA	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11608_Pvir_Dia2013_COI	DRWA-064	1	BE11608_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	CTTAGTGGCATTAATTCGDAATCC	130.93076
BE11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50872	WGS84	BE11608	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11608_Rhar_Sim2018_COI	DRWA-064	1	BE11608_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11608	Sample	BE11608	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-064	COI	45	BE11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-064	CTGCTGCTATTGCTCATG	130.93076
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11614_Asen_Wil2025_COI	DRWA-066	1	BE11614_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	GTTGTTGTTACAACACATGCTCTTTTG	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11614_Cjap_Sim2018_COI	DRWA-066	1	BE11614_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11614_Esin_And2018_Cyb	DRWA-066	1	BE11614_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-066	Cytb	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	ACCCCTCCTCATATCCAACCA	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11614_Mgig_Bot2012_COI	DRWA-066	1	BE11614_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	TCTTATTCGTTGGAGACTTTATAACCCT	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11614_Mjap_Gib2022_COI	DRWA-066	1	BE11614_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	AATTTAATTGTGACTAGGCATGGTCTG	130.92805
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11615_Cmae_Bot2011_COI	DRWA-067	1	BE11615_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	ATGAACAGTCTATCCTCCTTTAG	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11615_Hsan_Wil2021_COI	DRWA-067	1	BE11615_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	CCTGGGCCGGTATAGTAGGT	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11615_Mros_Gib2022_COI	DRWA-067	1	BE11615_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11615_Msal_Bot2012_COI	DRWA-067	1	BE11615_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	GYTAGTTCCRATGATGTTAGCTG	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11615_Mstr_Wil2021_COI	DRWA-067	1	BE11615_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	GTAGTGGTAACTACTCATGCTTTA	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11615_Pvir_Dia2013_COI	DRWA-067	1	BE11615_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	CTTAGTGGCATTAATTCGDAATCC	130.92913
BE11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50766	WGS84	BE11615	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11615_Rhar_Sim2018_COI	DRWA-067	1	BE11615_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11615	Sample	BE11615	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-067	COI	45	BE11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-067	CTGCTGCTATTGCTCATG	130.92913
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11617_Aamu_Bax2006_COI	DRWA-069	1	BE11617_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	GCACAACCGGGATCTTTACTTC	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11617_Pcan_Bot2011_IGS	DRWA-069	1	BE11617_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-069	IGS	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	CGTAATCCTCAGTACTGGCTA	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11617_Pper_Dia2013_COI	DRWA-069	1	BE11617_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	CTTAGTGGCATTAATTCGDAATCC	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11617_Sspa_Oph2007_COI	DRWA-069	1	BE11617_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	GAAGGCCTTCCGATGATC	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11617_Upin_Bot2011_COI	DRWA-069	1	BE11617_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	CTTTAATTACAGCGTTTTTATTGTTGT	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11617_Vgib_Wil2025_COI	DRWA-069	1	BE11617_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	CCCCTCTTTACAAGTTCTTTAGGGT	130.92765
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11618_Asen_Wil2025_COI	DRWA-070	1	BE11618_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	GTTGTTGTTACAACACATGCTCTTTTG	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11618_Cjap_Sim2018_COI	DRWA-070	1	BE11618_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11618_Esin_And2018_Cyb	DRWA-070	1	BE11618_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-070	Cytb	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	ACCCCTCCTCATATCCAACCA	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11618_Mgig_Bot2012_COI	DRWA-070	1	BE11618_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	TCTTATTCGTTGGAGACTTTATAACCCT	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11618_Mjap_Gib2022_COI	DRWA-070	1	BE11618_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	AATTTAATTGTGACTAGGCATGGTCTG	130.92862
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11619_Cmae_Bot2011_COI	DRWA-071	1	BE11619_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	ATGAACAGTCTATCCTCCTTTAG	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11619_Mros_Gib2022_COI	DRWA-071	1	BE11619_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11619_Msal_Bot2012_COI	DRWA-071	1	BE11619_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	GYTAGTTCCRATGATGTTAGCTG	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11619_Mstr_Wil2021_COI	DRWA-071	1	BE11619_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	GTAGTGGTAACTACTCATGCTTTA	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11619_Pvir_Dia2013_COI	DRWA-071	1	BE11619_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	CTTAGTGGCATTAATTCGDAATCC	130.88164
BE11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49636	WGS84	BE11619	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11619_Rhar_Sim2018_COI	DRWA-071	1	BE11619_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11619	Sample	BE11619	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-071	COI	45	BE11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-071	CTGCTGCTATTGCTCATG	130.88164
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11620_Aamu_Bax2006_COI	DRWA-072	1	BE11620_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	GCACAACCGGGATCTTTACTTC	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11620_Pcan_Bot2011_IGS	DRWA-072	1	BE11620_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-072	IGS	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	CGTAATCCTCAGTACTGGCTA	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11620_Pper_Dia2013_COI	DRWA-072	1	BE11620_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	CTTAGTGGCATTAATTCGDAATCC	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11620_Sspa_Oph2007_COI	DRWA-072	1	BE11620_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	GAAGGCCTTCCGATGATC	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11620_Upin_Bot2011_COI	DRWA-072	1	BE11620_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	CTTTAATTACAGCGTTTTTATTGTTGT	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11620_Vgib_Wil2025_COI	DRWA-072	1	BE11620_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	CCCCTCTTTACAAGTTCTTTAGGGT	130.8886
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11621_Asen_Wil2025_COI	DRWA-073	1	BE11621_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	GTTGTTGTTACAACACATGCTCTTTTG	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11621_Cjap_Sim2018_COI	DRWA-073	1	BE11621_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11621_Esin_And2018_Cyb	DRWA-073	1	BE11621_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-073	Cytb	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	ACCCCTCCTCATATCCAACCA	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11621_Mgig_Bot2012_COI	DRWA-073	1	BE11621_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	TCTTATTCGTTGGAGACTTTATAACCCT	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11621_Mjap_Gib2022_COI	DRWA-073	1	BE11621_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	AATTTAATTGTGACTAGGCATGGTCTG	130.89041
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11622_Cmae_Bot2011_COI	DRWA-074	1	BE11622_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	ATGAACAGTCTATCCTCCTTTAG	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11622_Hsan_Wil2021_COI	DRWA-074	1	BE11622_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	CCTGGGCCGGTATAGTAGGT	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11622_Mros_Gib2022_COI	DRWA-074	1	BE11622_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11622_Msal_Bot2012_COI	DRWA-074	1	BE11622_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	GYTAGTTCCRATGATGTTAGCTG	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11622_Mstr_Wil2021_COI	DRWA-074	1	BE11622_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	GTAGTGGTAACTACTCATGCTTTA	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11622_Pvir_Dia2013_COI	DRWA-074	1	BE11622_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	CTTAGTGGCATTAATTCGDAATCC	130.89165
BE11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49649	WGS84	BE11622	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11622_Rhar_Sim2018_COI	DRWA-074	1	BE11622_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11622	Sample	BE11622	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-074	COI	45	BE11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-074	CTGCTGCTATTGCTCATG	130.89165
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11624_Aamu_Bax2006_COI	DRWA-076	1	BE11624_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	GCACAACCGGGATCTTTACTTC	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11624_Pcan_Bot2011_IGS	DRWA-076	1	BE11624_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-076	IGS	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	CGTAATCCTCAGTACTGGCTA	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11624_Pper_Dia2013_COI	DRWA-076	1	BE11624_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	CTTAGTGGCATTAATTCGDAATCC	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11624_Sspa_Oph2007_COI	DRWA-076	1	BE11624_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	GAAGGCCTTCCGATGATC	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11624_Upin_Bot2011_COI	DRWA-076	1	BE11624_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	CTTTAATTACAGCGTTTTTATTGTTGT	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11624_Vgib_Wil2025_COI	DRWA-076	1	BE11624_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	CCCCTCTTTACAAGTTCTTTAGGGT	130.88109
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11625_Asen_Wil2025_COI	DRWA-077	1	BE11625_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	GTTGTTGTTACAACACATGCTCTTTTG	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11625_Cjap_Sim2018_COI	DRWA-077	1	BE11625_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11625_Esin_And2018_Cyb	DRWA-077	1	BE11625_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-077	Cytb	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	ACCCCTCCTCATATCCAACCA	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11625_Mgig_Bot2012_COI	DRWA-077	1	BE11625_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	TCTTATTCGTTGGAGACTTTATAACCCT	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11625_Mjap_Gib2022_COI	DRWA-077	1	BE11625_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	AATTTAATTGTGACTAGGCATGGTCTG	130.88243
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11626_Cmae_Bot2011_COI	DRWA-078	1	BE11626_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	ATGAACAGTCTATCCTCCTTTAG	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11626_Hsan_Wil2021_COI	DRWA-078	1	BE11626_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	CCTGGGCCGGTATAGTAGGT	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11626_Mros_Gib2022_COI	DRWA-078	1	BE11626_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11626_Msal_Bot2012_COI	DRWA-078	1	BE11626_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	GYTAGTTCCRATGATGTTAGCTG	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11626_Mstr_Wil2021_COI	DRWA-078	1	BE11626_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	GTAGTGGTAACTACTCATGCTTTA	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11626_Pvir_Dia2013_COI	DRWA-078	1	BE11626_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	CTTAGTGGCATTAATTCGDAATCC	130.88409
BE11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11626	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11626_Rhar_Sim2018_COI	DRWA-078	1	BE11626_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11626	Sample	BE11626	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-078	COI	45	BE11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-078	CTGCTGCTATTGCTCATG	130.88409
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11628_Aamu_Bax2006_COI	DRWA-080	1	BE11628_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	GCACAACCGGGATCTTTACTTC	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11628_Pcan_Bot2011_IGS	DRWA-080	1	BE11628_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-080	IGS	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	CGTAATCCTCAGTACTGGCTA	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11628_Pper_Dia2013_COI	DRWA-080	1	BE11628_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	CTTAGTGGCATTAATTCGDAATCC	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11628_Sspa_Oph2007_COI	DRWA-080	1	BE11628_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	GAAGGCCTTCCGATGATC	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11628_Upin_Bot2011_COI	DRWA-080	1	BE11628_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	CTTTAATTACAGCGTTTTTATTGTTGT	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11628_Vgib_Wil2025_COI	DRWA-080	1	BE11628_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	CCCCTCTTTACAAGTTCTTTAGGGT	130.88966
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11629_Asen_Wil2025_COI	DRWA-081	1	BE11629_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	GTTGTTGTTACAACACATGCTCTTTTG	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11629_Cjap_Sim2018_COI	DRWA-081	1	BE11629_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11629_Esin_And2018_Cyb	DRWA-081	1	BE11629_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-081	Cytb	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	ACCCCTCCTCATATCCAACCA	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11629_Mgig_Bot2012_COI	DRWA-081	1	BE11629_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	TCTTATTCGTTGGAGACTTTATAACCCT	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11629_Mjap_Gib2022_COI	DRWA-081	1	BE11629_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	AATTTAATTGTGACTAGGCATGGTCTG	130.87884
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11631_Aamu_Bax2006_COI	DRWA-083	1	BE11631_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	GCACAACCGGGATCTTTACTTC	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11631_Pcan_Bot2011_IGS	DRWA-083	1	BE11631_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-083	IGS	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	CGTAATCCTCAGTACTGGCTA	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11631_Pper_Dia2013_COI	DRWA-083	1	BE11631_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	CTTAGTGGCATTAATTCGDAATCC	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11631_Sspa_Oph2007_COI	DRWA-083	1	BE11631_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	GAAGGCCTTCCGATGATC	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11631_Upin_Bot2011_COI	DRWA-083	1	BE11631_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	CTTTAATTACAGCGTTTTTATTGTTGT	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11631_Vgib_Wil2025_COI	DRWA-083	1	BE11631_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	CCCCTCTTTACAAGTTCTTTAGGGT	130.88173
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11632_Asen_Wil2025_COI	DRWA-084	1	BE11632_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	GTTGTTGTTACAACACATGCTCTTTTG	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11632_Cjap_Sim2018_COI	DRWA-084	1	BE11632_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11632_Esin_And2018_Cyb	DRWA-084	1	BE11632_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-084	Cytb	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	ACCCCTCCTCATATCCAACCA	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11632_Mgig_Bot2012_COI	DRWA-084	1	BE11632_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	TCTTATTCGTTGGAGACTTTATAACCCT	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11632_Mjap_Gib2022_COI	DRWA-084	1	BE11632_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	AATTTAATTGTGACTAGGCATGGTCTG	130.87667
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11633_Cmae_Bot2011_COI	DRWA-085	1	BE11633_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	ATGAACAGTCTATCCTCCTTTAG	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11633_Hsan_Wil2021_COI	DRWA-085	1	BE11633_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	CCTGGGCCGGTATAGTAGGT	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11633_Mros_Gib2022_COI	DRWA-085	1	BE11633_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11633_Msal_Bot2012_COI	DRWA-085	1	BE11633_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	GYTAGTTCCRATGATGTTAGCTG	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11633_Mstr_Wil2021_COI	DRWA-085	1	BE11633_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	GTAGTGGTAACTACTCATGCTTTA	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11633_Pvir_Dia2013_COI	DRWA-085	1	BE11633_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	CTTAGTGGCATTAATTCGDAATCC	130.87809
BE11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49255	WGS84	BE11633	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11633_Rhar_Sim2018_COI	DRWA-085	1	BE11633_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11633	Sample	BE11633	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-085	COI	45	BE11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-085	CTGCTGCTATTGCTCATG	130.87809
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11635_Aamu_Bax2006_COI	DRWA-087	1	BE11635_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	GCACAACCGGGATCTTTACTTC	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11635_Pcan_Bot2011_IGS	DRWA-087	1	BE11635_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-087	IGS	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	CGTAATCCTCAGTACTGGCTA	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11635_Pper_Dia2013_COI	DRWA-087	1	BE11635_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	CTTAGTGGCATTAATTCGDAATCC	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11635_Sspa_Oph2007_COI	DRWA-087	1	BE11635_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	GAAGGCCTTCCGATGATC	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11635_Upin_Bot2011_COI	DRWA-087	1	BE11635_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	CTTTAATTACAGCGTTTTTATTGTTGT	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11635_Vgib_Wil2025_COI	DRWA-087	1	BE11635_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	CCCCTCTTTACAAGTTCTTTAGGGT	130.88106
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11636_Asen_Wil2025_COI	DRWA-088	1	BE11636_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	GTTGTTGTTACAACACATGCTCTTTTG	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11636_Cjap_Sim2018_COI	DRWA-088	1	BE11636_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11636_Esin_And2018_Cyb	DRWA-088	1	BE11636_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-088	Cytb	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	ACCCCTCCTCATATCCAACCA	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11636_Mgig_Bot2012_COI	DRWA-088	1	BE11636_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	TCTTATTCGTTGGAGACTTTATAACCCT	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11636_Mjap_Gib2022_COI	DRWA-088	1	BE11636_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	AATTTAATTGTGACTAGGCATGGTCTG	130.87464
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11637_Cmae_Bot2011_COI	DRWA-089	1	BE11637_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	ATGAACAGTCTATCCTCCTTTAG	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11637_Hsan_Wil2021_COI	DRWA-089	1	BE11637_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	CCTGGGCCGGTATAGTAGGT	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11637_Mros_Gib2022_COI	DRWA-089	1	BE11637_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11637_Msal_Bot2012_COI	DRWA-089	1	BE11637_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	GYTAGTTCCRATGATGTTAGCTG	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11637_Mstr_Wil2021_COI	DRWA-089	1	BE11637_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	GTAGTGGTAACTACTCATGCTTTA	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11637_Pvir_Dia2013_COI	DRWA-089	1	BE11637_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	CTTAGTGGCATTAATTCGDAATCC	130.8761
BE11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49143	WGS84	BE11637	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11637_Rhar_Sim2018_COI	DRWA-089	1	BE11637_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11637	Sample	BE11637	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-089	COI	45	BE11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-089	CTGCTGCTATTGCTCATG	130.8761
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11639_Aamu_Bax2006_COI	DRWA-091	1	BE11639_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	GCACAACCGGGATCTTTACTTC	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11639_Pcan_Bot2011_IGS	DRWA-091	1	BE11639_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-091	IGS	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	CGTAATCCTCAGTACTGGCTA	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11639_Pper_Dia2013_COI	DRWA-091	1	BE11639_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	CTTAGTGGCATTAATTCGDAATCC	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11639_Sspa_Oph2007_COI	DRWA-091	1	BE11639_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	GAAGGCCTTCCGATGATC	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11639_Upin_Bot2011_COI	DRWA-091	1	BE11639_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	CTTTAATTACAGCGTTTTTATTGTTGT	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11639_Vgib_Wil2025_COI	DRWA-091	1	BE11639_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	CCCCTCTTTACAAGTTCTTTAGGGT	130.87904
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11640_Cmae_Bot2011_COI	DRWA-092	1	BE11640_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	ATGAACAGTCTATCCTCCTTTAG	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11640_Hsan_Wil2021_COI	DRWA-092	1	BE11640_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	CCTGGGCCGGTATAGTAGGT	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11640_Mros_Gib2022_COI	DRWA-092	1	BE11640_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11640_Msal_Bot2012_COI	DRWA-092	1	BE11640_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	GYTAGTTCCRATGATGTTAGCTG	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11640_Mstr_Wil2021_COI	DRWA-092	1	BE11640_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	GTAGTGGTAACTACTCATGCTTTA	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11640_Pvir_Dia2013_COI	DRWA-092	1	BE11640_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	CTTAGTGGCATTAATTCGDAATCC	130.87405
BE11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49013	WGS84	BE11640	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11640_Rhar_Sim2018_COI	DRWA-092	1	BE11640_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11640	Sample	BE11640	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-092	COI	45	BE11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-092	CTGCTGCTATTGCTCATG	130.87405
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11642_Aamu_Bax2006_COI	DRWA-094	1	BE11642_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	GCACAACCGGGATCTTTACTTC	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11642_Pcan_Bot2011_IGS	DRWA-094	1	BE11642_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-094	IGS	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	CGTAATCCTCAGTACTGGCTA	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11642_Pper_Dia2013_COI	DRWA-094	1	BE11642_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	CTTAGTGGCATTAATTCGDAATCC	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11642_Sspa_Oph2007_COI	DRWA-094	1	BE11642_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	GAAGGCCTTCCGATGATC	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11642_Upin_Bot2011_COI	DRWA-094	1	BE11642_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	CTTTAATTACAGCGTTTTTATTGTTGT	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11642_Vgib_Wil2025_COI	DRWA-094	1	BE11642_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	CCCCTCTTTACAAGTTCTTTAGGGT	130.8769
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11643_Asen_Wil2025_COI	DRWA-095	1	BE11643_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	GTTGTTGTTACAACACATGCTCTTTTG	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11643_Cjap_Sim2018_COI	DRWA-095	1	BE11643_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11643_Esin_And2018_Cyb	DRWA-095	1	BE11643_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-095	Cytb	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	ACCCCTCCTCATATCCAACCA	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11643_Mgig_Bot2012_COI	DRWA-095	1	BE11643_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	TCTTATTCGTTGGAGACTTTATAACCCT	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11643_Mjap_Gib2022_COI	DRWA-095	1	BE11643_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	AATTTAATTGTGACTAGGCATGGTCTG	130.87313
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11644_Cmae_Bot2011_COI	DRWA-096	1	BE11644_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	ATGAACAGTCTATCCTCCTTTAG	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11644_Hsan_Wil2021_COI	DRWA-096	1	BE11644_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	CCTGGGCCGGTATAGTAGGT	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11644_Mros_Gib2022_COI	DRWA-096	1	BE11644_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11644_Msal_Bot2012_COI	DRWA-096	1	BE11644_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	GYTAGTTCCRATGATGTTAGCTG	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11644_Mstr_Wil2021_COI	DRWA-096	1	BE11644_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	GTAGTGGTAACTACTCATGCTTTA	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11644_Pvir_Dia2013_COI	DRWA-096	1	BE11644_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	CTTAGTGGCATTAATTCGDAATCC	130.87454
BE11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48891	WGS84	BE11644	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11644_Rhar_Sim2018_COI	DRWA-096	1	BE11644_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11644	Sample	BE11644	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-096	COI	45	BE11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-096	CTGCTGCTATTGCTCATG	130.87454
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11646_Aamu_Bax2006_COI	DRWA-098	1	BE11646_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	GCACAACCGGGATCTTTACTTC	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11646_Pcan_Bot2011_IGS	DRWA-098	1	BE11646_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-098	IGS	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	CGTAATCCTCAGTACTGGCTA	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11646_Pper_Dia2013_COI	DRWA-098	1	BE11646_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	CTTAGTGGCATTAATTCGDAATCC	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11646_Sspa_Oph2007_COI	DRWA-098	1	BE11646_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	GAAGGCCTTCCGATGATC	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11646_Upin_Bot2011_COI	DRWA-098	1	BE11646_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	CTTTAATTACAGCGTTTTTATTGTTGT	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11646_Vgib_Wil2025_COI	DRWA-098	1	BE11646_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	CCCCTCTTTACAAGTTCTTTAGGGT	130.87388
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11647_Asen_Wil2025_COI	DRWA-099	1	BE11647_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	GTTGTTGTTACAACACATGCTCTTTTG	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11647_Cjap_Sim2018_COI	DRWA-099	1	BE11647_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11647_Esin_And2018_Cyb	DRWA-099	1	BE11647_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-099	Cytb	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	ACCCCTCCTCATATCCAACCA	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11647_Mgig_Bot2012_COI	DRWA-099	1	BE11647_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	TCTTATTCGTTGGAGACTTTATAACCCT	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11647_Mjap_Gib2022_COI	DRWA-099	1	BE11647_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	AATTTAATTGTGACTAGGCATGGTCTG	130.87343
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11648_Cmae_Bot2011_COI	DRWA-100	1	BE11648_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	ATGAACAGTCTATCCTCCTTTAG	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11648_Hsan_Wil2021_COI	DRWA-100	1	BE11648_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	CCTGGGCCGGTATAGTAGGT	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11648_Mros_Gib2022_COI	DRWA-100	1	BE11648_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11648_Msal_Bot2012_COI	DRWA-100	1	BE11648_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	GYTAGTTCCRATGATGTTAGCTG	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11648_Mstr_Wil2021_COI	DRWA-100	1	BE11648_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	GTAGTGGTAACTACTCATGCTTTA	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11648_Pvir_Dia2013_COI	DRWA-100	1	BE11648_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	CTTAGTGGCATTAATTCGDAATCC	130.87492
BE11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48643	WGS84	BE11648	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11648_Rhar_Sim2018_COI	DRWA-100	1	BE11648_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11648	Sample	BE11648	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-100	COI	45	BE11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-100	CTGCTGCTATTGCTCATG	130.87492
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11650_Asen_Wil2025_COI	DRWA-102	1	BE11650_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	GTTGTTGTTACAACACATGCTCTTTTG	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11650_Cjap_Sim2018_COI	DRWA-102	1	BE11650_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11650_Esin_And2018_Cyb	DRWA-102	1	BE11650_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-102	Cytb	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	ACCCCTCCTCATATCCAACCA	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11650_Mgig_Bot2012_COI	DRWA-102	1	BE11650_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	TCTTATTCGTTGGAGACTTTATAACCCT	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11650_Mjap_Gib2022_COI	DRWA-102	1	BE11650_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	AATTTAATTGTGACTAGGCATGGTCTG	130.87416
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11651_Cmae_Bot2011_COI	DRWA-103	1	BE11651_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	ATGAACAGTCTATCCTCCTTTAG	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11651_Hsan_Wil2021_COI	DRWA-103	1	BE11651_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	CCTGGGCCGGTATAGTAGGT	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11651_Mros_Gib2022_COI	DRWA-103	1	BE11651_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11651_Msal_Bot2012_COI	DRWA-103	1	BE11651_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	GYTAGTTCCRATGATGTTAGCTG	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11651_Mstr_Wil2021_COI	DRWA-103	1	BE11651_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	GTAGTGGTAACTACTCATGCTTTA	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11651_Pvir_Dia2013_COI	DRWA-103	1	BE11651_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	CTTAGTGGCATTAATTCGDAATCC	130.87559
BE11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48523	WGS84	BE11651	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11651_Rhar_Sim2018_COI	DRWA-103	1	BE11651_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11651	Sample	BE11651	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-103	COI	45	BE11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-103	CTGCTGCTATTGCTCATG	130.87559
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11653_Aamu_Bax2006_COI	DRWA-105	1	BE11653_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	GCACAACCGGGATCTTTACTTC	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11653_Pcan_Bot2011_IGS	DRWA-105	1	BE11653_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-105	IGS	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	CGTAATCCTCAGTACTGGCTA	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11653_Pper_Dia2013_COI	DRWA-105	1	BE11653_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	CTTAGTGGCATTAATTCGDAATCC	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11653_Sspa_Oph2007_COI	DRWA-105	1	BE11653_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	GAAGGCCTTCCGATGATC	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11653_Upin_Bot2011_COI	DRWA-105	1	BE11653_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	CTTTAATTACAGCGTTTTTATTGTTGT	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11653_Vgib_Wil2025_COI	DRWA-105	1	BE11653_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	CCCCTCTTTACAAGTTCTTTAGGGT	130.87621
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11797_Asen_Wil2025_COI	DRWB-100	1	BE11797_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	GTTGTTGTTACAACACATGCTCTTTTG	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11797_Cjap_Sim2018_COI	DRWB-100	1	BE11797_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11797_Esin_And2018_Cyb	DRWB-100	1	BE11797_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-100	Cytb	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	ACCCCTCCTCATATCCAACCA	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11797_Mgig_Bot2012_COI	DRWB-100	1	BE11797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	TCTTATTCGTTGGAGACTTTATAACCCT	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11797_Mjap_Gib2022_COI	DRWB-100	1	BE11797_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	AATTTAATTGTGACTAGGCATGGTCTG	130.92721
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11798_Cmae_Bot2011_COI	DRWB-101	1	BE11798_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	ATGAACAGTCTATCCTCCTTTAG	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11798_Hsan_Wil2021_COI	DRWB-101	1	BE11798_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	CCTGGGCCGGTATAGTAGGT	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11798_Mros_Gib2022_COI	DRWB-101	1	BE11798_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11798_Msal_Bot2012_COI	DRWB-101	1	BE11798_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	GYTAGTTCCRATGATGTTAGCTG	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11798_Mstr_Wil2021_COI	DRWB-101	1	BE11798_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	GTAGTGGTAACTACTCATGCTTTA	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11798_Pvir_Dia2013_COI	DRWB-101	1	BE11798_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	CTTAGTGGCATTAATTCGDAATCC	130.9282
BE11798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50792	WGS84	BE11798	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11798_Rhar_Sim2018_COI	DRWB-101	1	BE11798_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11798	Sample	BE11798	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-101	COI	45	BE11798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-101	CTGCTGCTATTGCTCATG	130.9282
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11800_Aamu_Bax2006_COI	DRWB-103	1	BE11800_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	GCACAACCGGGATCTTTACTTC	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11800_Pcan_Bot2011_IGS	DRWB-103	1	BE11800_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-103	IGS	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	CGTAATCCTCAGTACTGGCTA	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11800_Pper_Dia2013_COI	DRWB-103	1	BE11800_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	CTTAGTGGCATTAATTCGDAATCC	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11800_Sspa_Oph2007_COI	DRWB-103	1	BE11800_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	GAAGGCCTTCCGATGATC	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11800_Upin_Bot2011_COI	DRWB-103	1	BE11800_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	CTTTAATTACAGCGTTTTTATTGTTGT	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11800_Vgib_Wil2025_COI	DRWB-103	1	BE11800_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	CCCCTCTTTACAAGTTCTTTAGGGT	130.93014
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11801_Asen_Wil2025_COI	DRWB-104	1	BE11801_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	GTTGTTGTTACAACACATGCTCTTTTG	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11801_Cjap_Sim2018_COI	DRWB-104	1	BE11801_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11801_Esin_And2018_Cyb	DRWB-104	1	BE11801_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-104	Cytb	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	ACCCCTCCTCATATCCAACCA	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11801_Mgig_Bot2012_COI	DRWB-104	1	BE11801_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	TCTTATTCGTTGGAGACTTTATAACCCT	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11801_Mjap_Gib2022_COI	DRWB-104	1	BE11801_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	AATTTAATTGTGACTAGGCATGGTCTG	130.92768
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11802_Cmae_Bot2011_COI	DRWB-105	1	BE11802_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	ATGAACAGTCTATCCTCCTTTAG	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11802_Hsan_Wil2021_COI	DRWB-105	1	BE11802_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	CCTGGGCCGGTATAGTAGGT	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11802_Mros_Gib2022_COI	DRWB-105	1	BE11802_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11802_Msal_Bot2012_COI	DRWB-105	1	BE11802_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	GYTAGTTCCRATGATGTTAGCTG	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11802_Mstr_Wil2021_COI	DRWB-105	1	BE11802_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	GTAGTGGTAACTACTCATGCTTTA	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11802_Pvir_Dia2013_COI	DRWB-105	1	BE11802_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	CTTAGTGGCATTAATTCGDAATCC	130.92863
BE11802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5069	WGS84	BE11802	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11802_Rhar_Sim2018_COI	DRWB-105	1	BE11802_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11802	Sample	BE11802	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-105	COI	45	BE11802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-105	CTGCTGCTATTGCTCATG	130.92863
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11804_Aamu_Bax2006_COI	DRWB-107	1	BE11804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	GCACAACCGGGATCTTTACTTC	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11804_Pcan_Bot2011_IGS	DRWB-107	1	BE11804_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-107	IGS	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	CGTAATCCTCAGTACTGGCTA	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11804_Pper_Dia2013_COI	DRWB-107	1	BE11804_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	CTTAGTGGCATTAATTCGDAATCC	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11804_Sspa_Oph2007_COI	DRWB-107	1	BE11804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	GAAGGCCTTCCGATGATC	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11804_Upin_Bot2011_COI	DRWB-107	1	BE11804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	CTTTAATTACAGCGTTTTTATTGTTGT	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11804_Vgib_Wil2025_COI	DRWB-107	1	BE11804_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	CCCCTCTTTACAAGTTCTTTAGGGT	130.88878
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11805_Asen_Wil2025_COI	DRWB-108	1	BE11805_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	GTTGTTGTTACAACACATGCTCTTTTG	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11805_Cjap_Sim2018_COI	DRWB-108	1	BE11805_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11805_Esin_And2018_Cyb	DRWB-108	1	BE11805_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-108	Cytb	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	ACCCCTCCTCATATCCAACCA	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11805_Mgig_Bot2012_COI	DRWB-108	1	BE11805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	TCTTATTCGTTGGAGACTTTATAACCCT	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11805_Mjap_Gib2022_COI	DRWB-108	1	BE11805_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	AATTTAATTGTGACTAGGCATGGTCTG	130.89031
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11806_Cmae_Bot2011_COI	DRWB-109	1	BE11806_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	ATGAACAGTCTATCCTCCTTTAG	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11806_Hsan_Wil2021_COI	DRWB-109	1	BE11806_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	CCTGGGCCGGTATAGTAGGT	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11806_Mros_Gib2022_COI	DRWB-109	1	BE11806_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11806_Msal_Bot2012_COI	DRWB-109	1	BE11806_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	GYTAGTTCCRATGATGTTAGCTG	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11806_Mstr_Wil2021_COI	DRWB-109	1	BE11806_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	GTAGTGGTAACTACTCATGCTTTA	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11806_Pvir_Dia2013_COI	DRWB-109	1	BE11806_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	CTTAGTGGCATTAATTCGDAATCC	130.89198
BE11806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49657	WGS84	BE11806	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11806_Rhar_Sim2018_COI	DRWB-109	1	BE11806_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11806	Sample	BE11806	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-109	COI	45	BE11806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-109	CTGCTGCTATTGCTCATG	130.89198
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11808_Aamu_Bax2006_COI	DRWB-111	1	BE11808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	GCACAACCGGGATCTTTACTTC	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11808_Pcan_Bot2011_IGS	DRWB-111	1	BE11808_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-111	IGS	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	CGTAATCCTCAGTACTGGCTA	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11808_Pper_Dia2013_COI	DRWB-111	1	BE11808_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	CTTAGTGGCATTAATTCGDAATCC	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11808_Sspa_Oph2007_COI	DRWB-111	1	BE11808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	GAAGGCCTTCCGATGATC	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11808_Upin_Bot2011_COI	DRWB-111	1	BE11808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	CTTTAATTACAGCGTTTTTATTGTTGT	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11808_Vgib_Wil2025_COI	DRWB-111	1	BE11808_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	CCCCTCTTTACAAGTTCTTTAGGGT	130.88104
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11809_Asen_Wil2025_COI	DRWB-112	1	BE11809_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	GTTGTTGTTACAACACATGCTCTTTTG	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11809_Cjap_Sim2018_COI	DRWB-112	1	BE11809_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11809_Esin_And2018_Cyb	DRWB-112	1	BE11809_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-112	Cytb	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	ACCCCTCCTCATATCCAACCA	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11809_Mgig_Bot2012_COI	DRWB-112	1	BE11809_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	TCTTATTCGTTGGAGACTTTATAACCCT	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11809_Mjap_Gib2022_COI	DRWB-112	1	BE11809_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	AATTTAATTGTGACTAGGCATGGTCTG	130.88251
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11811_Aamu_Bax2006_COI	DRWB-114	1	BE11811_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	GCACAACCGGGATCTTTACTTC	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11811_Pcan_Bot2011_IGS	DRWB-114	1	BE11811_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-114	IGS	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	CGTAATCCTCAGTACTGGCTA	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11811_Pper_Dia2013_COI	DRWB-114	1	BE11811_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	CTTAGTGGCATTAATTCGDAATCC	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11811_Sspa_Oph2007_COI	DRWB-114	1	BE11811_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	GAAGGCCTTCCGATGATC	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11811_Upin_Bot2011_COI	DRWB-114	1	BE11811_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	CTTTAATTACAGCGTTTTTATTGTTGT	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11811_Vgib_Wil2025_COI	DRWB-114	1	BE11811_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	CCCCTCTTTACAAGTTCTTTAGGGT	130.88842
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11812_Asen_Wil2025_COI	DRWB-115	1	BE11812_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	GTTGTTGTTACAACACATGCTCTTTTG	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11812_Cjap_Sim2018_COI	DRWB-115	1	BE11812_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11812_Esin_And2018_Cyb	DRWB-115	1	BE11812_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-115	Cytb	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	ACCCCTCCTCATATCCAACCA	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11812_Mgig_Bot2012_COI	DRWB-115	1	BE11812_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	TCTTATTCGTTGGAGACTTTATAACCCT	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11812_Mjap_Gib2022_COI	DRWB-115	1	BE11812_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	AATTTAATTGTGACTAGGCATGGTCTG	130.88975
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11813_Cmae_Bot2011_COI	DRWB-116	1	BE11813_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	ATGAACAGTCTATCCTCCTTTAG	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11813_Hsan_Wil2021_COI	DRWB-116	1	BE11813_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	CCTGGGCCGGTATAGTAGGT	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11813_Mros_Gib2022_COI	DRWB-116	1	BE11813_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11813_Msal_Bot2012_COI	DRWB-116	1	BE11813_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	GYTAGTTCCRATGATGTTAGCTG	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11813_Mstr_Wil2021_COI	DRWB-116	1	BE11813_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	GTAGTGGTAACTACTCATGCTTTA	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11813_Pvir_Dia2013_COI	DRWB-116	1	BE11813_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	CTTAGTGGCATTAATTCGDAATCC	130.87898
BE11813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49378	WGS84	BE11813	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11813_Rhar_Sim2018_COI	DRWB-116	1	BE11813_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11813	Sample	BE11813	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-116	COI	45	BE11813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-116	CTGCTGCTATTGCTCATG	130.87898
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11815_Aamu_Bax2006_COI	DRWB-118	1	BE11815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	GCACAACCGGGATCTTTACTTC	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11815_Pcan_Bot2011_IGS	DRWB-118	1	BE11815_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-118	IGS	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	CGTAATCCTCAGTACTGGCTA	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11815_Pper_Dia2013_COI	DRWB-118	1	BE11815_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	CTTAGTGGCATTAATTCGDAATCC	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11815_Sspa_Oph2007_COI	DRWB-118	1	BE11815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	GAAGGCCTTCCGATGATC	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11815_Upin_Bot2011_COI	DRWB-118	1	BE11815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	CTTTAATTACAGCGTTTTTATTGTTGT	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11815_Vgib_Wil2025_COI	DRWB-118	1	BE11815_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	CCCCTCTTTACAAGTTCTTTAGGGT	130.88173
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11816_Asen_Wil2025_COI	DRWB-119	1	BE11816_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	GTTGTTGTTACAACACATGCTCTTTTG	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11816_Cjap_Sim2018_COI	DRWB-119	1	BE11816_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11816_Esin_And2018_Cyb	DRWB-119	1	BE11816_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-119	Cytb	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	ACCCCTCCTCATATCCAACCA	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11816_Mgig_Bot2012_COI	DRWB-119	1	BE11816_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	TCTTATTCGTTGGAGACTTTATAACCCT	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11816_Mjap_Gib2022_COI	DRWB-119	1	BE11816_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	AATTTAATTGTGACTAGGCATGGTCTG	130.87668
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11817_Cmae_Bot2011_COI	DRWB-120	1	BE11817_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	ATGAACAGTCTATCCTCCTTTAG	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11817_Hsan_Wil2021_COI	DRWB-120	1	BE11817_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	CCTGGGCCGGTATAGTAGGT	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11817_Mros_Gib2022_COI	DRWB-120	1	BE11817_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11817_Msal_Bot2012_COI	DRWB-120	1	BE11817_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	GYTAGTTCCRATGATGTTAGCTG	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11817_Mstr_Wil2021_COI	DRWB-120	1	BE11817_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	GTAGTGGTAACTACTCATGCTTTA	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11817_Pvir_Dia2013_COI	DRWB-120	1	BE11817_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	CTTAGTGGCATTAATTCGDAATCC	130.87813
BE11817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49255	WGS84	BE11817	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11817_Rhar_Sim2018_COI	DRWB-120	1	BE11817_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11817	Sample	BE11817	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-120	COI	45	BE11817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-120	CTGCTGCTATTGCTCATG	130.87813
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11819_Aamu_Bax2006_COI	DRWB-122	1	BE11819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	GCACAACCGGGATCTTTACTTC	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11819_Pcan_Bot2011_IGS	DRWB-122	1	BE11819_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-122	IGS	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	CGTAATCCTCAGTACTGGCTA	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11819_Pper_Dia2013_COI	DRWB-122	1	BE11819_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	CTTAGTGGCATTAATTCGDAATCC	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11819_Sspa_Oph2007_COI	DRWB-122	1	BE11819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	GAAGGCCTTCCGATGATC	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11819_Upin_Bot2011_COI	DRWB-122	1	BE11819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	CTTTAATTACAGCGTTTTTATTGTTGT	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11819_Vgib_Wil2025_COI	DRWB-122	1	BE11819_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	CCCCTCTTTACAAGTTCTTTAGGGT	130.88122
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11820_Cmae_Bot2011_COI	DRWB-123	1	BE11820_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	ATGAACAGTCTATCCTCCTTTAG	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11820_Hsan_Wil2021_COI	DRWB-123	1	BE11820_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	CCTGGGCCGGTATAGTAGGT	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11820_Mros_Gib2022_COI	DRWB-123	1	BE11820_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11820_Msal_Bot2012_COI	DRWB-123	1	BE11820_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	GYTAGTTCCRATGATGTTAGCTG	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11820_Mstr_Wil2021_COI	DRWB-123	1	BE11820_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	GTAGTGGTAACTACTCATGCTTTA	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11820_Pvir_Dia2013_COI	DRWB-123	1	BE11820_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	CTTAGTGGCATTAATTCGDAATCC	130.87459
BE11820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49121	WGS84	BE11820	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11820_Rhar_Sim2018_COI	DRWB-123	1	BE11820_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11820	Sample	BE11820	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-123	COI	45	BE11820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-123	CTGCTGCTATTGCTCATG	130.87459
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11822_Aamu_Bax2006_COI	DRWB-125	1	BE11822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	GCACAACCGGGATCTTTACTTC	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11822_Pcan_Bot2011_IGS	DRWB-125	1	BE11822_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-125	IGS	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	CGTAATCCTCAGTACTGGCTA	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11822_Pper_Dia2013_COI	DRWB-125	1	BE11822_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	CTTAGTGGCATTAATTCGDAATCC	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11822_Sspa_Oph2007_COI	DRWB-125	1	BE11822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	GAAGGCCTTCCGATGATC	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11822_Upin_Bot2011_COI	DRWB-125	1	BE11822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	CTTTAATTACAGCGTTTTTATTGTTGT	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11822_Vgib_Wil2025_COI	DRWB-125	1	BE11822_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	CCCCTCTTTACAAGTTCTTTAGGGT	130.87747
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11823_Asen_Wil2025_COI	DRWB-126	1	BE11823_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	GTTGTTGTTACAACACATGCTCTTTTG	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11823_Cjap_Sim2018_COI	DRWB-126	1	BE11823_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11823_Esin_And2018_Cyb	DRWB-126	1	BE11823_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-126	Cytb	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	ACCCCTCCTCATATCCAACCA	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11823_Mgig_Bot2012_COI	DRWB-126	1	BE11823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	TCTTATTCGTTGGAGACTTTATAACCCT	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11823_Mjap_Gib2022_COI	DRWB-126	1	BE11823_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	AATTTAATTGTGACTAGGCATGGTCTG	130.87892
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11824_Cmae_Bot2011_COI	DRWB-127	1	BE11824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	ATGAACAGTCTATCCTCCTTTAG	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11824_Hsan_Wil2021_COI	DRWB-127	1	BE11824_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	CCTGGGCCGGTATAGTAGGT	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11824_Mros_Gib2022_COI	DRWB-127	1	BE11824_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11824_Msal_Bot2012_COI	DRWB-127	1	BE11824_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	GYTAGTTCCRATGATGTTAGCTG	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11824_Mstr_Wil2021_COI	DRWB-127	1	BE11824_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	GTAGTGGTAACTACTCATGCTTTA	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11824_Pvir_Dia2013_COI	DRWB-127	1	BE11824_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	CTTAGTGGCATTAATTCGDAATCC	130.87409
BE11824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49002	WGS84	BE11824	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11824_Rhar_Sim2018_COI	DRWB-127	1	BE11824_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11824	Sample	BE11824	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-127	COI	45	BE11824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-127	CTGCTGCTATTGCTCATG	130.87409
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11826_Aamu_Bax2006_COI	DRWB-129	1	BE11826_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	GCACAACCGGGATCTTTACTTC	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11826_Pcan_Bot2011_IGS	DRWB-129	1	BE11826_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-129	IGS	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	CGTAATCCTCAGTACTGGCTA	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11826_Pper_Dia2013_COI	DRWB-129	1	BE11826_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	CTTAGTGGCATTAATTCGDAATCC	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11826_Sspa_Oph2007_COI	DRWB-129	1	BE11826_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	GAAGGCCTTCCGATGATC	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11826_Upin_Bot2011_COI	DRWB-129	1	BE11826_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	CTTTAATTACAGCGTTTTTATTGTTGT	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11826_Vgib_Wil2025_COI	DRWB-129	1	BE11826_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	CCCCTCTTTACAAGTTCTTTAGGGT	130.87695
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11827_Asen_Wil2025_COI	DRWB-130	1	BE11827_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	GTTGTTGTTACAACACATGCTCTTTTG	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11827_Cjap_Sim2018_COI	DRWB-130	1	BE11827_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11827_Esin_And2018_Cyb	DRWB-130	1	BE11827_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-130	Cytb	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	ACCCCTCCTCATATCCAACCA	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11827_Mgig_Bot2012_COI	DRWB-130	1	BE11827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	TCTTATTCGTTGGAGACTTTATAACCCT	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11827_Mjap_Gib2022_COI	DRWB-130	1	BE11827_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	AATTTAATTGTGACTAGGCATGGTCTG	130.87335
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11828_Cmae_Bot2011_COI	DRWB-131	1	BE11828_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	ATGAACAGTCTATCCTCCTTTAG	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11828_Hsan_Wil2021_COI	DRWB-131	1	BE11828_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	CCTGGGCCGGTATAGTAGGT	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11828_Mros_Gib2022_COI	DRWB-131	1	BE11828_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11828_Msal_Bot2012_COI	DRWB-131	1	BE11828_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	GYTAGTTCCRATGATGTTAGCTG	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11828_Mstr_Wil2021_COI	DRWB-131	1	BE11828_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	GTAGTGGTAACTACTCATGCTTTA	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11828_Pvir_Dia2013_COI	DRWB-131	1	BE11828_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	CTTAGTGGCATTAATTCGDAATCC	130.87468
BE11828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48879	WGS84	BE11828	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11828_Rhar_Sim2018_COI	DRWB-131	1	BE11828_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11828	Sample	BE11828	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-131	COI	45	BE11828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-131	CTGCTGCTATTGCTCATG	130.87468
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11830_Asen_Wil2025_COI	DRWB-133	1	BE11830_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	GTTGTTGTTACAACACATGCTCTTTTG	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11830_Cjap_Sim2018_COI	DRWB-133	1	BE11830_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11830_Esin_And2018_Cyb	DRWB-133	1	BE11830_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-133	Cytb	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	ACCCCTCCTCATATCCAACCA	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11830_Mgig_Bot2012_COI	DRWB-133	1	BE11830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	TCTTATTCGTTGGAGACTTTATAACCCT	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11830_Mjap_Gib2022_COI	DRWB-133	1	BE11830_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	AATTTAATTGTGACTAGGCATGGTCTG	130.87392
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11831_Cmae_Bot2011_COI	DRWB-134	1	BE11831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	ATGAACAGTCTATCCTCCTTTAG	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11831_Hsan_Wil2021_COI	DRWB-134	1	BE11831_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	CCTGGGCCGGTATAGTAGGT	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11831_Mros_Gib2022_COI	DRWB-134	1	BE11831_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11831_Msal_Bot2012_COI	DRWB-134	1	BE11831_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	GYTAGTTCCRATGATGTTAGCTG	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11831_Mstr_Wil2021_COI	DRWB-134	1	BE11831_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	GTAGTGGTAACTACTCATGCTTTA	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11831_Pvir_Dia2013_COI	DRWB-134	1	BE11831_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	CTTAGTGGCATTAATTCGDAATCC	130.87337
BE11831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48634	WGS84	BE11831	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11831_Rhar_Sim2018_COI	DRWB-134	1	BE11831_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11831	Sample	BE11831	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-134	COI	45	BE11831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-134	CTGCTGCTATTGCTCATG	130.87337
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11877_Aamu_Bax2006_COI	DRWB-072	1	BE11877_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	GCACAACCGGGATCTTTACTTC	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11877_Pcan_Bot2011_IGS	DRWB-072	1	BE11877_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-072	IGS	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	CGTAATCCTCAGTACTGGCTA	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11877_Pper_Dia2013_COI	DRWB-072	1	BE11877_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	CTTAGTGGCATTAATTCGDAATCC	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11877_Sspa_Oph2007_COI	DRWB-072	1	BE11877_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	GAAGGCCTTCCGATGATC	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11877_Upin_Bot2011_COI	DRWB-072	1	BE11877_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	CTTTAATTACAGCGTTTTTATTGTTGT	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11877_Vgib_Wil2025_COI	DRWB-072	1	BE11877_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	CCCCTCTTTACAAGTTCTTTAGGGT	130.93003
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11878_Asen_Wil2025_COI	DRWB-073	1	BE11878_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	GTTGTTGTTACAACACATGCTCTTTTG	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11878_Cjap_Sim2018_COI	DRWB-073	1	BE11878_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11878_Esin_And2018_Cyb	DRWB-073	1	BE11878_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-073	Cytb	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	ACCCCTCCTCATATCCAACCA	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11878_Mgig_Bot2012_COI	DRWB-073	1	BE11878_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	TCTTATTCGTTGGAGACTTTATAACCCT	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11878_Mjap_Gib2022_COI	DRWB-073	1	BE11878_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	AATTTAATTGTGACTAGGCATGGTCTG	130.93104
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11879_Cmae_Bot2011_COI	DRWB-074	1	BE11879_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	ATGAACAGTCTATCCTCCTTTAG	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11879_Hsan_Wil2021_COI	DRWB-074	1	BE11879_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	CCTGGGCCGGTATAGTAGGT	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11879_Mros_Gib2022_COI	DRWB-074	1	BE11879_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11879_Msal_Bot2012_COI	DRWB-074	1	BE11879_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	GYTAGTTCCRATGATGTTAGCTG	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11879_Mstr_Wil2021_COI	DRWB-074	1	BE11879_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	GTAGTGGTAACTACTCATGCTTTA	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11879_Pvir_Dia2013_COI	DRWB-074	1	BE11879_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	CTTAGTGGCATTAATTCGDAATCC	130.93201
BE11879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51478	WGS84	BE11879	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11879_Rhar_Sim2018_COI	DRWB-074	1	BE11879_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11879	Sample	BE11879	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-074	COI	45	BE11879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-074	CTGCTGCTATTGCTCATG	130.93201
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11880_Aamu_Bax2006_COI	DRWB-075	1	BE11880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	GCACAACCGGGATCTTTACTTC	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11880_Pcan_Bot2011_IGS	DRWB-075	1	BE11880_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-075	IGS	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	CGTAATCCTCAGTACTGGCTA	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11880_Pper_Dia2013_COI	DRWB-075	1	BE11880_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	CTTAGTGGCATTAATTCGDAATCC	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11880_Sspa_Oph2007_COI	DRWB-075	1	BE11880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	GAAGGCCTTCCGATGATC	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11880_Upin_Bot2011_COI	DRWB-075	1	BE11880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	CTTTAATTACAGCGTTTTTATTGTTGT	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11880_Vgib_Wil2025_COI	DRWB-075	1	BE11880_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	CCCCTCTTTACAAGTTCTTTAGGGT	130.93056
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11881_Asen_Wil2025_COI	DRWB-076	1	BE11881_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	GTTGTTGTTACAACACATGCTCTTTTG	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11881_Cjap_Sim2018_COI	DRWB-076	1	BE11881_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11881_Esin_And2018_Cyb	DRWB-076	1	BE11881_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-076	Cytb	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	ACCCCTCCTCATATCCAACCA	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11881_Mgig_Bot2012_COI	DRWB-076	1	BE11881_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	TCTTATTCGTTGGAGACTTTATAACCCT	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11881_Mjap_Gib2022_COI	DRWB-076	1	BE11881_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	AATTTAATTGTGACTAGGCATGGTCTG	130.93156
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11882_Cmae_Bot2011_COI	DRWB-077	1	BE11882_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	ATGAACAGTCTATCCTCCTTTAG	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11882_Hsan_Wil2021_COI	DRWB-077	1	BE11882_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	CCTGGGCCGGTATAGTAGGT	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11882_Mros_Gib2022_COI	DRWB-077	1	BE11882_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11882_Msal_Bot2012_COI	DRWB-077	1	BE11882_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	GYTAGTTCCRATGATGTTAGCTG	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11882_Mstr_Wil2021_COI	DRWB-077	1	BE11882_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	GTAGTGGTAACTACTCATGCTTTA	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11882_Pvir_Dia2013_COI	DRWB-077	1	BE11882_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	CTTAGTGGCATTAATTCGDAATCC	130.93254
BE11882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5139	WGS84	BE11882	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11882_Rhar_Sim2018_COI	DRWB-077	1	BE11882_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11882	Sample	BE11882	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-077	COI	45	BE11882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-077	CTGCTGCTATTGCTCATG	130.93254
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11884_Aamu_Bax2006_COI	DRWB-079	1	BE11884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	GCACAACCGGGATCTTTACTTC	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11884_Pcan_Bot2011_IGS	DRWB-079	1	BE11884_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-079	IGS	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	CGTAATCCTCAGTACTGGCTA	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11884_Pper_Dia2013_COI	DRWB-079	1	BE11884_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	CTTAGTGGCATTAATTCGDAATCC	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11884_Sspa_Oph2007_COI	DRWB-079	1	BE11884_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	GAAGGCCTTCCGATGATC	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11884_Upin_Bot2011_COI	DRWB-079	1	BE11884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	CTTTAATTACAGCGTTTTTATTGTTGT	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11884_Vgib_Wil2025_COI	DRWB-079	1	BE11884_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	CCCCTCTTTACAAGTTCTTTAGGGT	130.93108
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11885_Asen_Wil2025_COI	DRWB-080	1	BE11885_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	GTTGTTGTTACAACACATGCTCTTTTG	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11885_Cjap_Sim2018_COI	DRWB-080	1	BE11885_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11885_Esin_And2018_Cyb	DRWB-080	1	BE11885_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-080	Cytb	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	ACCCCTCCTCATATCCAACCA	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11885_Mgig_Bot2012_COI	DRWB-080	1	BE11885_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	TCTTATTCGTTGGAGACTTTATAACCCT	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11885_Mjap_Gib2022_COI	DRWB-080	1	BE11885_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	AATTTAATTGTGACTAGGCATGGTCTG	130.93206
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11886_Cmae_Bot2011_COI	DRWB-081	1	BE11886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	ATGAACAGTCTATCCTCCTTTAG	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11886_Hsan_Wil2021_COI	DRWB-081	1	BE11886_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	CCTGGGCCGGTATAGTAGGT	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11886_Mros_Gib2022_COI	DRWB-081	1	BE11886_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11886_Msal_Bot2012_COI	DRWB-081	1	BE11886_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	GYTAGTTCCRATGATGTTAGCTG	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11886_Mstr_Wil2021_COI	DRWB-081	1	BE11886_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	GTAGTGGTAACTACTCATGCTTTA	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11886_Pvir_Dia2013_COI	DRWB-081	1	BE11886_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	CTTAGTGGCATTAATTCGDAATCC	130.92961
BE11886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51215	WGS84	BE11886	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11886_Rhar_Sim2018_COI	DRWB-081	1	BE11886_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11886	Sample	BE11886	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-081	COI	45	BE11886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-081	CTGCTGCTATTGCTCATG	130.92961
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11888_Aamu_Bax2006_COI	DRWB-083	1	BE11888_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	GCACAACCGGGATCTTTACTTC	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11888_Pcan_Bot2011_IGS	DRWB-083	1	BE11888_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-083	IGS	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	CGTAATCCTCAGTACTGGCTA	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11888_Pper_Dia2013_COI	DRWB-083	1	BE11888_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	CTTAGTGGCATTAATTCGDAATCC	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11888_Sspa_Oph2007_COI	DRWB-083	1	BE11888_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	GAAGGCCTTCCGATGATC	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11888_Upin_Bot2011_COI	DRWB-083	1	BE11888_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	CTTTAATTACAGCGTTTTTATTGTTGT	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11888_Vgib_Wil2025_COI	DRWB-083	1	BE11888_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	CCCCTCTTTACAAGTTCTTTAGGGT	130.9316
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11889_Asen_Wil2025_COI	DRWB-084	1	BE11889_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	GTTGTTGTTACAACACATGCTCTTTTG	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11889_Cjap_Sim2018_COI	DRWB-084	1	BE11889_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11889_Esin_And2018_Cyb	DRWB-084	1	BE11889_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-084	Cytb	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	ACCCCTCCTCATATCCAACCA	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11889_Mgig_Bot2012_COI	DRWB-084	1	BE11889_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	TCTTATTCGTTGGAGACTTTATAACCCT	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11889_Mjap_Gib2022_COI	DRWB-084	1	BE11889_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	AATTTAATTGTGACTAGGCATGGTCTG	130.92909
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11891_Aamu_Bax2006_COI	DRWB-086	1	BE11891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	GCACAACCGGGATCTTTACTTC	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11891_Pcan_Bot2011_IGS	DRWB-086	1	BE11891_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-086	IGS	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	CGTAATCCTCAGTACTGGCTA	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11891_Pper_Dia2013_COI	DRWB-086	1	BE11891_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	CTTAGTGGCATTAATTCGDAATCC	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11891_Sspa_Oph2007_COI	DRWB-086	1	BE11891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	GAAGGCCTTCCGATGATC	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11891_Upin_Bot2011_COI	DRWB-086	1	BE11891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	CTTTAATTACAGCGTTTTTATTGTTGT	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11891_Vgib_Wil2025_COI	DRWB-086	1	BE11891_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	CCCCTCTTTACAAGTTCTTTAGGGT	130.93112
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11892_Asen_Wil2025_COI	DRWB-087	1	BE11892_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	GTTGTTGTTACAACACATGCTCTTTTG	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11892_Cjap_Sim2018_COI	DRWB-087	1	BE11892_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11892_Esin_And2018_Cyb	DRWB-087	1	BE11892_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-087	Cytb	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	ACCCCTCCTCATATCCAACCA	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11892_Mgig_Bot2012_COI	DRWB-087	1	BE11892_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	TCTTATTCGTTGGAGACTTTATAACCCT	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11892_Mjap_Gib2022_COI	DRWB-087	1	BE11892_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	AATTTAATTGTGACTAGGCATGGTCTG	130.93215
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11893_Cmae_Bot2011_COI	DRWB-088	1	BE11893_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	ATGAACAGTCTATCCTCCTTTAG	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11893_Hsan_Wil2021_COI	DRWB-088	1	BE11893_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	CCTGGGCCGGTATAGTAGGT	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11893_Mros_Gib2022_COI	DRWB-088	1	BE11893_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11893_Msal_Bot2012_COI	DRWB-088	1	BE11893_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	GYTAGTTCCRATGATGTTAGCTG	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11893_Mstr_Wil2021_COI	DRWB-088	1	BE11893_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	GTAGTGGTAACTACTCATGCTTTA	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11893_Pvir_Dia2013_COI	DRWB-088	1	BE11893_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	CTTAGTGGCATTAATTCGDAATCC	130.92859
BE11893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5104	WGS84	BE11893	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11893_Rhar_Sim2018_COI	DRWB-088	1	BE11893_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11893	Sample	BE11893	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-088	COI	45	BE11893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-088	CTGCTGCTATTGCTCATG	130.92859
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11895_Aamu_Bax2006_COI	DRWB-090	1	BE11895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	GCACAACCGGGATCTTTACTTC	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11895_Pcan_Bot2011_IGS	DRWB-090	1	BE11895_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-090	IGS	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	CGTAATCCTCAGTACTGGCTA	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11895_Pper_Dia2013_COI	DRWB-090	1	BE11895_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	CTTAGTGGCATTAATTCGDAATCC	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11895_Sspa_Oph2007_COI	DRWB-090	1	BE11895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	GAAGGCCTTCCGATGATC	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11895_Upin_Bot2011_COI	DRWB-090	1	BE11895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	CTTTAATTACAGCGTTTTTATTGTTGT	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11895_Vgib_Wil2025_COI	DRWB-090	1	BE11895_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	CCCCTCTTTACAAGTTCTTTAGGGT	130.93065
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11896_Asen_Wil2025_COI	DRWB-091	1	BE11896_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	GTTGTTGTTACAACACATGCTCTTTTG	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11896_Cjap_Sim2018_COI	DRWB-091	1	BE11896_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11896_Esin_And2018_Cyb	DRWB-091	1	BE11896_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-091	Cytb	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	ACCCCTCCTCATATCCAACCA	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11896_Mgig_Bot2012_COI	DRWB-091	1	BE11896_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	TCTTATTCGTTGGAGACTTTATAACCCT	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11896_Mjap_Gib2022_COI	DRWB-091	1	BE11896_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	AATTTAATTGTGACTAGGCATGGTCTG	130.93164
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11897_Cmae_Bot2011_COI	DRWB-092	1	BE11897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	ATGAACAGTCTATCCTCCTTTAG	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11897_Hsan_Wil2021_COI	DRWB-092	1	BE11897_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	CCTGGGCCGGTATAGTAGGT	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11897_Mros_Gib2022_COI	DRWB-092	1	BE11897_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11897_Msal_Bot2012_COI	DRWB-092	1	BE11897_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	GYTAGTTCCRATGATGTTAGCTG	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11897_Mstr_Wil2021_COI	DRWB-092	1	BE11897_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	GTAGTGGTAACTACTCATGCTTTA	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11897_Pvir_Dia2013_COI	DRWB-092	1	BE11897_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	CTTAGTGGCATTAATTCGDAATCC	130.9281
BE11897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50954	WGS84	BE11897	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11897_Rhar_Sim2018_COI	DRWB-092	1	BE11897_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11897	Sample	BE11897	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-092	COI	45	BE11897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-092	CTGCTGCTATTGCTCATG	130.9281
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11899_Aamu_Bax2006_COI	DRWB-094	1	BE11899_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	GCACAACCGGGATCTTTACTTC	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11899_Pcan_Bot2011_IGS	DRWB-094	1	BE11899_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-094	IGS	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	CGTAATCCTCAGTACTGGCTA	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11899_Pper_Dia2013_COI	DRWB-094	1	BE11899_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	CTTAGTGGCATTAATTCGDAATCC	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11899_Sspa_Oph2007_COI	DRWB-094	1	BE11899_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	GAAGGCCTTCCGATGATC	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11899_Upin_Bot2011_COI	DRWB-094	1	BE11899_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	CTTTAATTACAGCGTTTTTATTGTTGT	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11899_Vgib_Wil2025_COI	DRWB-094	1	BE11899_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	CCCCTCTTTACAAGTTCTTTAGGGT	130.93013
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11900_Asen_Wil2025_COI	DRWB-095	1	BE11900_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	GTTGTTGTTACAACACATGCTCTTTTG	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11900_Cjap_Sim2018_COI	DRWB-095	1	BE11900_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11900_Esin_And2018_Cyb	DRWB-095	1	BE11900_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-095	Cytb	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	ACCCCTCCTCATATCCAACCA	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11900_Mgig_Bot2012_COI	DRWB-095	1	BE11900_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	TCTTATTCGTTGGAGACTTTATAACCCT	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11900_Mjap_Gib2022_COI	DRWB-095	1	BE11900_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	AATTTAATTGTGACTAGGCATGGTCTG	130.93115
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11901_Cmae_Bot2011_COI	DRWB-096	1	BE11901_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	ATGAACAGTCTATCCTCCTTTAG	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11901_Hsan_Wil2021_COI	DRWB-096	1	BE11901_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	CCTGGGCCGGTATAGTAGGT	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11901_Mros_Gib2022_COI	DRWB-096	1	BE11901_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11901_Msal_Bot2012_COI	DRWB-096	1	BE11901_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	GYTAGTTCCRATGATGTTAGCTG	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11901_Mstr_Wil2021_COI	DRWB-096	1	BE11901_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	GTAGTGGTAACTACTCATGCTTTA	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11901_Pvir_Dia2013_COI	DRWB-096	1	BE11901_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	CTTAGTGGCATTAATTCGDAATCC	130.92764
BE11901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50869	WGS84	BE11901	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11901_Rhar_Sim2018_COI	DRWB-096	1	BE11901_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11901	Sample	BE11901	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-096	COI	45	BE11901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-096	CTGCTGCTATTGCTCATG	130.92764
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11903_Aamu_Bax2006_COI	DRWB-098	1	BE11903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	GCACAACCGGGATCTTTACTTC	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11903_Pcan_Bot2011_IGS	DRWB-098	1	BE11903_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-098	IGS	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	CGTAATCCTCAGTACTGGCTA	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11903_Pper_Dia2013_COI	DRWB-098	1	BE11903_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	CTTAGTGGCATTAATTCGDAATCC	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11903_Sspa_Oph2007_COI	DRWB-098	1	BE11903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	GAAGGCCTTCCGATGATC	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11903_Upin_Bot2011_COI	DRWB-098	1	BE11903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	CTTTAATTACAGCGTTTTTATTGTTGT	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11903_Vgib_Wil2025_COI	DRWB-098	1	BE11903_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	CCCCTCTTTACAAGTTCTTTAGGGT	130.92962
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11904_Asen_Wil2025_COI	DRWB-099	1	BE11904_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	GTTGTTGTTACAACACATGCTCTTTTG	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11904_Cjap_Sim2018_COI	DRWB-099	1	BE11904_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11904_Esin_And2018_Cyb	DRWB-099	1	BE11904_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-099	Cytb	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	ACCCCTCCTCATATCCAACCA	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11904_Mgig_Bot2012_COI	DRWB-099	1	BE11904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	TCTTATTCGTTGGAGACTTTATAACCCT	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11904_Mjap_Gib2022_COI	DRWB-099	1	BE11904_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	AATTTAATTGTGACTAGGCATGGTCTG	130.93073
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11914_Aamu_Bax2006_COI	GLTB-018	1	BE11914_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	GCACAACCGGGATCTTTACTTC	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11914_Pcan_Bot2011_IGS	GLTB-018	1	BE11914_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-018	IGS	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	CGTAATCCTCAGTACTGGCTA	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11914_Pper_Dia2013_COI	GLTB-018	1	BE11914_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	CTTAGTGGCATTAATTCGDAATCC	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11914_Sspa_Oph2007_COI	GLTB-018	1	BE11914_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	GAAGGCCTTCCGATGATC	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11914_Upin_Bot2011_COI	GLTB-018	1	BE11914_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	CTTTAATTACAGCGTTTTTATTGTTGT	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11914_Vgib_Wil2025_COI	GLTB-018	1	BE11914_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	CCCCTCTTTACAAGTTCTTTAGGGT	151.32254
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11915_Asen_Wil2025_COI	GLTB-012	1	BE11915_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	GTTGTTGTTACAACACATGCTCTTTTG	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11915_Cjap_Sim2018_COI	GLTB-012	1	BE11915_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11915_Esin_And2018_Cyb	GLTB-012	1	BE11915_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-012	Cytb	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	ACCCCTCCTCATATCCAACCA	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11915_Mgig_Bot2012_COI	GLTB-012	1	BE11915_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	TCTTATTCGTTGGAGACTTTATAACCCT	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11915_Mjap_Gib2022_COI	GLTB-012	1	BE11915_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	AATTTAATTGTGACTAGGCATGGTCTG	151.32407
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11916_Cmae_Bot2011_COI	GLTB-024	1	BE11916_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	ATGAACAGTCTATCCTCCTTTAG	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11916_Hsan_Wil2021_COI	GLTB-024	1	BE11916_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	CCTGGGCCGGTATAGTAGGT	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11916_Mros_Gib2022_COI	GLTB-024	1	BE11916_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11916_Msal_Bot2012_COI	GLTB-024	1	BE11916_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	GYTAGTTCCRATGATGTTAGCTG	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11916_Mstr_Wil2021_COI	GLTB-024	1	BE11916_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	GTAGTGGTAACTACTCATGCTTTA	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11916_Pvir_Dia2013_COI	GLTB-024	1	BE11916_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	CTTAGTGGCATTAATTCGDAATCC	151.31444
BE11916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85022	WGS84	BE11916	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11916_Rhar_Sim2018_COI	GLTB-024	1	BE11916_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11916	Sample	BE11916	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-024	COI	45	BE11916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-024	CTGCTGCTATTGCTCATG	151.31444
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11918_Aamu_Bax2006_COI	GLTB-029	1	BE11918_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	GCACAACCGGGATCTTTACTTC	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11918_Pcan_Bot2011_IGS	GLTB-029	1	BE11918_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-029	IGS	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	CGTAATCCTCAGTACTGGCTA	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11918_Pper_Dia2013_COI	GLTB-029	1	BE11918_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	CTTAGTGGCATTAATTCGDAATCC	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11918_Sspa_Oph2007_COI	GLTB-029	1	BE11918_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	GAAGGCCTTCCGATGATC	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11918_Upin_Bot2011_COI	GLTB-029	1	BE11918_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	CTTTAATTACAGCGTTTTTATTGTTGT	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11918_Vgib_Wil2025_COI	GLTB-029	1	BE11918_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	CCCCTCTTTACAAGTTCTTTAGGGT	151.30871
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11919_Asen_Wil2025_COI	GLTB-005	1	BE11919_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	GTTGTTGTTACAACACATGCTCTTTTG	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11919_Cjap_Sim2018_COI	GLTB-005	1	BE11919_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11919_Esin_And2018_Cyb	GLTB-005	1	BE11919_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-005	Cytb	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	ACCCCTCCTCATATCCAACCA	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11919_Mgig_Bot2012_COI	GLTB-005	1	BE11919_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	TCTTATTCGTTGGAGACTTTATAACCCT	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11919_Mjap_Gib2022_COI	GLTB-005	1	BE11919_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	AATTTAATTGTGACTAGGCATGGTCTG	151.32604
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11921_Aamu_Bax2006_COI	GLTB-010	1	BE11921_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	GCACAACCGGGATCTTTACTTC	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11921_Pcan_Bot2011_IGS	GLTB-010	1	BE11921_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-010	IGS	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	CGTAATCCTCAGTACTGGCTA	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11921_Pper_Dia2013_COI	GLTB-010	1	BE11921_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	CTTAGTGGCATTAATTCGDAATCC	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11921_Sspa_Oph2007_COI	GLTB-010	1	BE11921_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	GAAGGCCTTCCGATGATC	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11921_Upin_Bot2011_COI	GLTB-010	1	BE11921_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	CTTTAATTACAGCGTTTTTATTGTTGT	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11921_Vgib_Wil2025_COI	GLTB-010	1	BE11921_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	CCCCTCTTTACAAGTTCTTTAGGGT	151.32044
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11922_Asen_Wil2025_COI	GLTB-011	1	BE11922_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	GTTGTTGTTACAACACATGCTCTTTTG	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11922_Cjap_Sim2018_COI	GLTB-011	1	BE11922_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11922_Esin_And2018_Cyb	GLTB-011	1	BE11922_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-011	Cytb	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	ACCCCTCCTCATATCCAACCA	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11922_Mgig_Bot2012_COI	GLTB-011	1	BE11922_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	TCTTATTCGTTGGAGACTTTATAACCCT	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11922_Mjap_Gib2022_COI	GLTB-011	1	BE11922_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	AATTTAATTGTGACTAGGCATGGTCTG	151.32207
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11923_Cmae_Bot2011_COI	GLTB-017	1	BE11923_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	ATGAACAGTCTATCCTCCTTTAG	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11923_Hsan_Wil2021_COI	GLTB-017	1	BE11923_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	CCTGGGCCGGTATAGTAGGT	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11923_Mros_Gib2022_COI	GLTB-017	1	BE11923_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11923_Msal_Bot2012_COI	GLTB-017	1	BE11923_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	GYTAGTTCCRATGATGTTAGCTG	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11923_Mstr_Wil2021_COI	GLTB-017	1	BE11923_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	GTAGTGGTAACTACTCATGCTTTA	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11923_Pvir_Dia2013_COI	GLTB-017	1	BE11923_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	CTTAGTGGCATTAATTCGDAATCC	151.32109
BE11923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85086	WGS84	BE11923	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11923_Rhar_Sim2018_COI	GLTB-017	1	BE11923_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11923	Sample	BE11923	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-017	COI	45	BE11923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-017	CTGCTGCTATTGCTCATG	151.32109
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11925_Aamu_Bax2006_COI	GLTB-022	1	BE11925_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	GCACAACCGGGATCTTTACTTC	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11925_Pcan_Bot2011_IGS	GLTB-022	1	BE11925_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-022	IGS	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	CGTAATCCTCAGTACTGGCTA	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11925_Pper_Dia2013_COI	GLTB-022	1	BE11925_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	CTTAGTGGCATTAATTCGDAATCC	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11925_Sspa_Oph2007_COI	GLTB-022	1	BE11925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	GAAGGCCTTCCGATGATC	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11925_Upin_Bot2011_COI	GLTB-022	1	BE11925_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	CTTTAATTACAGCGTTTTTATTGTTGT	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11925_Vgib_Wil2025_COI	GLTB-022	1	BE11925_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	CCCCTCTTTACAAGTTCTTTAGGGT	151.31132
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11926_Asen_Wil2025_COI	GLTB-016	1	BE11926_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	GTTGTTGTTACAACACATGCTCTTTTG	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11926_Cjap_Sim2018_COI	GLTB-016	1	BE11926_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11926_Esin_And2018_Cyb	GLTB-016	1	BE11926_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-016	Cytb	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	ACCCCTCCTCATATCCAACCA	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11926_Mgig_Bot2012_COI	GLTB-016	1	BE11926_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	TCTTATTCGTTGGAGACTTTATAACCCT	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11926_Mjap_Gib2022_COI	GLTB-016	1	BE11926_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	AATTTAATTGTGACTAGGCATGGTCTG	151.31957
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11927_Cmae_Bot2011_COI	GLTB-003	1	BE11927_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	ATGAACAGTCTATCCTCCTTTAG	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11927_Hsan_Wil2021_COI	GLTB-003	1	BE11927_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	CCTGGGCCGGTATAGTAGGT	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11927_Mros_Gib2022_COI	GLTB-003	1	BE11927_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11927_Msal_Bot2012_COI	GLTB-003	1	BE11927_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	GYTAGTTCCRATGATGTTAGCTG	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11927_Mstr_Wil2021_COI	GLTB-003	1	BE11927_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	GTAGTGGTAACTACTCATGCTTTA	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11927_Pvir_Dia2013_COI	GLTB-003	1	BE11927_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	CTTAGTGGCATTAATTCGDAATCC	151.32561
BE11927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85547	WGS84	BE11927	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11927_Rhar_Sim2018_COI	GLTB-003	1	BE11927_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11927	Sample	BE11927	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-003	COI	45	BE11927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-003	CTGCTGCTATTGCTCATG	151.32561
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11929_Aamu_Bax2006_COI	GLTB-009	1	BE11929_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	GCACAACCGGGATCTTTACTTC	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11929_Pcan_Bot2011_IGS	GLTB-009	1	BE11929_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-009	IGS	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	CGTAATCCTCAGTACTGGCTA	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11929_Pper_Dia2013_COI	GLTB-009	1	BE11929_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	CTTAGTGGCATTAATTCGDAATCC	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11929_Sspa_Oph2007_COI	GLTB-009	1	BE11929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	GAAGGCCTTCCGATGATC	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11929_Upin_Bot2011_COI	GLTB-009	1	BE11929_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	CTTTAATTACAGCGTTTTTATTGTTGT	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11929_Vgib_Wil2025_COI	GLTB-009	1	BE11929_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	CCCCTCTTTACAAGTTCTTTAGGGT	151.31877
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11930_Cmae_Bot2011_COI	GLTB-015	1	BE11930_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	ATGAACAGTCTATCCTCCTTTAG	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11930_Hsan_Wil2021_COI	GLTB-015	1	BE11930_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	CCTGGGCCGGTATAGTAGGT	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11930_Mros_Gib2022_COI	GLTB-015	1	BE11930_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11930_Msal_Bot2012_COI	GLTB-015	1	BE11930_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	GYTAGTTCCRATGATGTTAGCTG	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11930_Mstr_Wil2021_COI	GLTB-015	1	BE11930_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	GTAGTGGTAACTACTCATGCTTTA	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11930_Pvir_Dia2013_COI	GLTB-015	1	BE11930_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	CTTAGTGGCATTAATTCGDAATCC	151.31768
BE11930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85096	WGS84	BE11930	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11930_Rhar_Sim2018_COI	GLTB-015	1	BE11930_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11930	Sample	BE11930	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-015	COI	45	BE11930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-015	CTGCTGCTATTGCTCATG	151.31768
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11932_Aamu_Bax2006_COI	GLTB-021	1	BE11932_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	GCACAACCGGGATCTTTACTTC	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11932_Pcan_Bot2011_IGS	GLTB-021	1	BE11932_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-021	IGS	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	CGTAATCCTCAGTACTGGCTA	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11932_Pper_Dia2013_COI	GLTB-021	1	BE11932_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	CTTAGTGGCATTAATTCGDAATCC	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11932_Sspa_Oph2007_COI	GLTB-021	1	BE11932_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	GAAGGCCTTCCGATGATC	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11932_Upin_Bot2011_COI	GLTB-021	1	BE11932_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	CTTTAATTACAGCGTTTTTATTGTTGT	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11932_Vgib_Wil2025_COI	GLTB-021	1	BE11932_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	CCCCTCTTTACAAGTTCTTTAGGGT	151.3098
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11933_Asen_Wil2025_COI	GLTB-014	1	BE11933_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	GTTGTTGTTACAACACATGCTCTTTTG	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11933_Cjap_Sim2018_COI	GLTB-014	1	BE11933_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11933_Esin_And2018_Cyb	GLTB-014	1	BE11933_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-014	Cytb	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	ACCCCTCCTCATATCCAACCA	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11933_Mgig_Bot2012_COI	GLTB-014	1	BE11933_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	TCTTATTCGTTGGAGACTTTATAACCCT	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11933_Mjap_Gib2022_COI	GLTB-014	1	BE11933_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	AATTTAATTGTGACTAGGCATGGTCTG	151.31603
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11934_Cmae_Bot2011_COI	GLTB-008	1	BE11934_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	ATGAACAGTCTATCCTCCTTTAG	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11934_Hsan_Wil2021_COI	GLTB-008	1	BE11934_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	CCTGGGCCGGTATAGTAGGT	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11934_Mros_Gib2022_COI	GLTB-008	1	BE11934_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11934_Msal_Bot2012_COI	GLTB-008	1	BE11934_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	GYTAGTTCCRATGATGTTAGCTG	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11934_Mstr_Wil2021_COI	GLTB-008	1	BE11934_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	GTAGTGGTAACTACTCATGCTTTA	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11934_Pvir_Dia2013_COI	GLTB-008	1	BE11934_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	CTTAGTGGCATTAATTCGDAATCC	151.31702
BE11934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85268	WGS84	BE11934	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11934_Rhar_Sim2018_COI	GLTB-008	1	BE11934_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11934	Sample	BE11934	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-008	COI	45	BE11934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-008	CTGCTGCTATTGCTCATG	151.31702
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11936_Aamu_Bax2006_COI	GLTB-020	1	BE11936_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	GCACAACCGGGATCTTTACTTC	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11936_Pcan_Bot2011_IGS	GLTB-020	1	BE11936_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-020	IGS	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	CGTAATCCTCAGTACTGGCTA	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11936_Pper_Dia2013_COI	GLTB-020	1	BE11936_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	CTTAGTGGCATTAATTCGDAATCC	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11936_Sspa_Oph2007_COI	GLTB-020	1	BE11936_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	GAAGGCCTTCCGATGATC	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11936_Upin_Bot2011_COI	GLTB-020	1	BE11936_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	CTTTAATTACAGCGTTTTTATTGTTGT	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11936_Vgib_Wil2025_COI	GLTB-020	1	BE11936_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	CCCCTCTTTACAAGTTCTTTAGGGT	151.30812
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11937_Asen_Wil2025_COI	GLTB-019	1	BE11937_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	GTTGTTGTTACAACACATGCTCTTTTG	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11937_Cjap_Sim2018_COI	GLTB-019	1	BE11937_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11937_Esin_And2018_Cyb	GLTB-019	1	BE11937_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-019	Cytb	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	ACCCCTCCTCATATCCAACCA	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11937_Mgig_Bot2012_COI	GLTB-019	1	BE11937_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	TCTTATTCGTTGGAGACTTTATAACCCT	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11937_Mjap_Gib2022_COI	GLTB-019	1	BE11937_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	AATTTAATTGTGACTAGGCATGGTCTG	151.30599
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11938_Cmae_Bot2011_COI	GLTB-025	1	BE11938_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	ATGAACAGTCTATCCTCCTTTAG	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11938_Hsan_Wil2021_COI	GLTB-025	1	BE11938_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	CCTGGGCCGGTATAGTAGGT	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11938_Mros_Gib2022_COI	GLTB-025	1	BE11938_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11938_Msal_Bot2012_COI	GLTB-025	1	BE11938_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	GYTAGTTCCRATGATGTTAGCTG	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11938_Mstr_Wil2021_COI	GLTB-025	1	BE11938_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	GTAGTGGTAACTACTCATGCTTTA	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11938_Pvir_Dia2013_COI	GLTB-025	1	BE11938_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	CTTAGTGGCATTAATTCGDAATCC	151.31631
BE11938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84983	WGS84	BE11938	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11938_Rhar_Sim2018_COI	GLTB-025	1	BE11938_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11938	Sample	BE11938	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-025	COI	45	BE11938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-025	CTGCTGCTATTGCTCATG	151.31631
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11940_Asen_Wil2025_COI	GLTB-013	1	BE11940_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	GTTGTTGTTACAACACATGCTCTTTTG	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11940_Cjap_Sim2018_COI	GLTB-013	1	BE11940_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11940_Esin_And2018_Cyb	GLTB-013	1	BE11940_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-013	Cytb	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	ACCCCTCCTCATATCCAACCA	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11940_Mgig_Bot2012_COI	GLTB-013	1	BE11940_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	TCTTATTCGTTGGAGACTTTATAACCCT	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11940_Mjap_Gib2022_COI	GLTB-013	1	BE11940_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	AATTTAATTGTGACTAGGCATGGTCTG	151.30703
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11941_Cmae_Bot2011_COI	GLTB-001	1	BE11941_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	ATGAACAGTCTATCCTCCTTTAG	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11941_Mros_Gib2022_COI	GLTB-001	1	BE11941_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11941_Msal_Bot2012_COI	GLTB-001	1	BE11941_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	GYTAGTTCCRATGATGTTAGCTG	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11941_Mstr_Wil2021_COI	GLTB-001	1	BE11941_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	GTAGTGGTAACTACTCATGCTTTA	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11941_Pvir_Dia2013_COI	GLTB-001	1	BE11941_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	CTTAGTGGCATTAATTCGDAATCC	151.32441
BE11941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85701	WGS84	BE11941	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11941_Rhar_Sim2018_COI	GLTB-001	1	BE11941_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11941	Sample	BE11941	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-001	COI	45	BE11941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-001	CTGCTGCTATTGCTCATG	151.32441
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11943_Aamu_Bax2006_COI	GLTB-031	1	BE11943_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	GCACAACCGGGATCTTTACTTC	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11943_Pcan_Bot2011_IGS	GLTB-031	1	BE11943_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-031	IGS	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	CGTAATCCTCAGTACTGGCTA	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11943_Pper_Dia2013_COI	GLTB-031	1	BE11943_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	CTTAGTGGCATTAATTCGDAATCC	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11943_Sspa_Oph2007_COI	GLTB-031	1	BE11943_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	GAAGGCCTTCCGATGATC	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11943_Upin_Bot2011_COI	GLTB-031	1	BE11943_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	CTTTAATTACAGCGTTTTTATTGTTGT	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11943_Vgib_Wil2025_COI	GLTB-031	1	BE11943_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	CCCCTCTTTACAAGTTCTTTAGGGT	151.31176
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11944_Asen_Wil2025_COI	GLTB-032	1	BE11944_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	GTTGTTGTTACAACACATGCTCTTTTG	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11944_Cjap_Sim2018_COI	GLTB-032	1	BE11944_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11944_Esin_And2018_Cyb	GLTB-032	1	BE11944_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-032	Cytb	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	ACCCCTCCTCATATCCAACCA	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11944_Mgig_Bot2012_COI	GLTB-032	1	BE11944_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	TCTTATTCGTTGGAGACTTTATAACCCT	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11944_Mjap_Gib2022_COI	GLTB-032	1	BE11944_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	AATTTAATTGTGACTAGGCATGGTCTG	151.31378
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11945_Cmae_Bot2011_COI	GLTB-033	1	BE11945_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	ATGAACAGTCTATCCTCCTTTAG	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11945_Hsan_Wil2021_COI	GLTB-033	1	BE11945_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	CCTGGGCCGGTATAGTAGGT	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11945_Mros_Gib2022_COI	GLTB-033	1	BE11945_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11945_Msal_Bot2012_COI	GLTB-033	1	BE11945_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	GYTAGTTCCRATGATGTTAGCTG	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11945_Mstr_Wil2021_COI	GLTB-033	1	BE11945_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	GTAGTGGTAACTACTCATGCTTTA	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11945_Pvir_Dia2013_COI	GLTB-033	1	BE11945_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	CTTAGTGGCATTAATTCGDAATCC	151.31518
BE11945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84792	WGS84	BE11945	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11945_Rhar_Sim2018_COI	GLTB-033	1	BE11945_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11945	Sample	BE11945	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-033	COI	45	BE11945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-033	CTGCTGCTATTGCTCATG	151.31518
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11947_Aamu_Bax2006_COI	GLTB-035	1	BE11947_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	GCACAACCGGGATCTTTACTTC	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11947_Pcan_Bot2011_IGS	GLTB-035	1	BE11947_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-035	IGS	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	CGTAATCCTCAGTACTGGCTA	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11947_Pper_Dia2013_COI	GLTB-035	1	BE11947_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	CTTAGTGGCATTAATTCGDAATCC	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11947_Sspa_Oph2007_COI	GLTB-035	1	BE11947_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	GAAGGCCTTCCGATGATC	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11947_Upin_Bot2011_COI	GLTB-035	1	BE11947_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	CTTTAATTACAGCGTTTTTATTGTTGT	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11947_Vgib_Wil2025_COI	GLTB-035	1	BE11947_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	CCCCTCTTTACAAGTTCTTTAGGGT	151.31271
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11952_Cmae_Bot2011_COI	GLTB-041	1	BE11952_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	ATGAACAGTCTATCCTCCTTTAG	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11952_Hsan_Wil2021_COI	GLTB-041	1	BE11952_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	CCTGGGCCGGTATAGTAGGT	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11952_Mros_Gib2022_COI	GLTB-041	1	BE11952_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11952_Msal_Bot2012_COI	GLTB-041	1	BE11952_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	GYTAGTTCCRATGATGTTAGCTG	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11952_Mstr_Wil2021_COI	GLTB-041	1	BE11952_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	GTAGTGGTAACTACTCATGCTTTA	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11952_Pvir_Dia2013_COI	GLTB-041	1	BE11952_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	CTTAGTGGCATTAATTCGDAATCC	151.27678
BE11952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83401	WGS84	BE11952	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11952_Rhar_Sim2018_COI	GLTB-041	1	BE11952_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11952	Sample	BE11952	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-041	COI	45	BE11952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-041	CTGCTGCTATTGCTCATG	151.27678
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11954_Aamu_Bax2006_COI	GLTB-043	1	BE11954_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	GCACAACCGGGATCTTTACTTC	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11954_Pcan_Bot2011_IGS	GLTB-043	1	BE11954_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-043	IGS	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	CGTAATCCTCAGTACTGGCTA	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11954_Pper_Dia2013_COI	GLTB-043	1	BE11954_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	CTTAGTGGCATTAATTCGDAATCC	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11954_Sspa_Oph2007_COI	GLTB-043	1	BE11954_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	GAAGGCCTTCCGATGATC	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11954_Upin_Bot2011_COI	GLTB-043	1	BE11954_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	CTTTAATTACAGCGTTTTTATTGTTGT	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11954_Vgib_Wil2025_COI	GLTB-043	1	BE11954_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	CCCCTCTTTACAAGTTCTTTAGGGT	151.26832
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11955_Asen_Wil2025_COI	GLTB-044	1	BE11955_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	GTTGTTGTTACAACACATGCTCTTTTG	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11955_Cjap_Sim2018_COI	GLTB-044	1	BE11955_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11955_Esin_And2018_Cyb	GLTB-044	1	BE11955_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-044	Cytb	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	ACCCCTCCTCATATCCAACCA	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11955_Mgig_Bot2012_COI	GLTB-044	1	BE11955_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	TCTTATTCGTTGGAGACTTTATAACCCT	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11955_Mjap_Gib2022_COI	GLTB-044	1	BE11955_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	AATTTAATTGTGACTAGGCATGGTCTG	151.27042
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11956_Cmae_Bot2011_COI	GLTB-045	1	BE11956_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	ATGAACAGTCTATCCTCCTTTAG	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11956_Hsan_Wil2021_COI	GLTB-045	1	BE11956_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	CCTGGGCCGGTATAGTAGGT	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11956_Mros_Gib2022_COI	GLTB-045	1	BE11956_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11956_Msal_Bot2012_COI	GLTB-045	1	BE11956_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	GYTAGTTCCRATGATGTTAGCTG	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11956_Mstr_Wil2021_COI	GLTB-045	1	BE11956_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	GTAGTGGTAACTACTCATGCTTTA	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11956_Pvir_Dia2013_COI	GLTB-045	1	BE11956_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	CTTAGTGGCATTAATTCGDAATCC	151.27238
BE11956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83327	WGS84	BE11956	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11956_Rhar_Sim2018_COI	GLTB-045	1	BE11956_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11956	Sample	BE11956	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-045	COI	45	BE11956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-045	CTGCTGCTATTGCTCATG	151.27238
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11958_Aamu_Bax2006_COI	GLTB-053	1	BE11958_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	GCACAACCGGGATCTTTACTTC	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11958_Pcan_Bot2011_IGS	GLTB-053	1	BE11958_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-053	IGS	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	CGTAATCCTCAGTACTGGCTA	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11958_Pper_Dia2013_COI	GLTB-053	1	BE11958_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	CTTAGTGGCATTAATTCGDAATCC	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11958_Sspa_Oph2007_COI	GLTB-053	1	BE11958_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	GAAGGCCTTCCGATGATC	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11958_Upin_Bot2011_COI	GLTB-053	1	BE11958_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	CTTTAATTACAGCGTTTTTATTGTTGT	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11958_Vgib_Wil2025_COI	GLTB-053	1	BE11958_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	CCCCTCTTTACAAGTTCTTTAGGGT	151.26226
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11959_Asen_Wil2025_COI	GLTB-047	1	BE11959_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	GTTGTTGTTACAACACATGCTCTTTTG	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11959_Cjap_Sim2018_COI	GLTB-047	1	BE11959_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11959_Esin_And2018_Cyb	GLTB-047	1	BE11959_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-047	Cytb	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	ACCCCTCCTCATATCCAACCA	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11959_Mgig_Bot2012_COI	GLTB-047	1	BE11959_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	TCTTATTCGTTGGAGACTTTATAACCCT	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11959_Mjap_Gib2022_COI	GLTB-047	1	BE11959_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	AATTTAATTGTGACTAGGCATGGTCTG	151.27558
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11961_Aamu_Bax2006_COI	GLTB-049	1	BE11961_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	GCACAACCGGGATCTTTACTTC	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11961_Pcan_Bot2011_IGS	GLTB-049	1	BE11961_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-049	IGS	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	CGTAATCCTCAGTACTGGCTA	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11961_Pper_Dia2013_COI	GLTB-049	1	BE11961_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	CTTAGTGGCATTAATTCGDAATCC	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11961_Sspa_Oph2007_COI	GLTB-049	1	BE11961_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	GAAGGCCTTCCGATGATC	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11961_Upin_Bot2011_COI	GLTB-049	1	BE11961_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	CTTTAATTACAGCGTTTTTATTGTTGT	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11961_Vgib_Wil2025_COI	GLTB-049	1	BE11961_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	CCCCTCTTTACAAGTTCTTTAGGGT	151.26732
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11962_Asen_Wil2025_COI	GLTB-050	1	BE11962_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	GTTGTTGTTACAACACATGCTCTTTTG	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11962_Cjap_Sim2018_COI	GLTB-050	1	BE11962_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11962_Esin_And2018_Cyb	GLTB-050	1	BE11962_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-050	Cytb	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	ACCCCTCCTCATATCCAACCA	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11962_Mgig_Bot2012_COI	GLTB-050	1	BE11962_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	TCTTATTCGTTGGAGACTTTATAACCCT	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11962_Mjap_Gib2022_COI	GLTB-050	1	BE11962_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	AATTTAATTGTGACTAGGCATGGTCTG	151.26908
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11963_Cmae_Bot2011_COI	GLTB-051	1	BE11963_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	ATGAACAGTCTATCCTCCTTTAG	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11963_Hsan_Wil2021_COI	GLTB-051	1	BE11963_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	CCTGGGCCGGTATAGTAGGT	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11963_Mros_Gib2022_COI	GLTB-051	1	BE11963_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11963_Msal_Bot2012_COI	GLTB-051	1	BE11963_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	GYTAGTTCCRATGATGTTAGCTG	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11963_Mstr_Wil2021_COI	GLTB-051	1	BE11963_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	GTAGTGGTAACTACTCATGCTTTA	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11963_Pvir_Dia2013_COI	GLTB-051	1	BE11963_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	CTTAGTGGCATTAATTCGDAATCC	151.27092
BE11963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83193	WGS84	BE11963	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11963_Rhar_Sim2018_COI	GLTB-051	1	BE11963_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11963	Sample	BE11963	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-051	COI	45	BE11963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-051	CTGCTGCTATTGCTCATG	151.27092
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11965_Aamu_Bax2006_COI	GLTB-054	1	BE11965_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	GCACAACCGGGATCTTTACTTC	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11965_Pcan_Bot2011_IGS	GLTB-054	1	BE11965_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-054	IGS	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	CGTAATCCTCAGTACTGGCTA	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11965_Pper_Dia2013_COI	GLTB-054	1	BE11965_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	CTTAGTGGCATTAATTCGDAATCC	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11965_Sspa_Oph2007_COI	GLTB-054	1	BE11965_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	GAAGGCCTTCCGATGATC	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11965_Upin_Bot2011_COI	GLTB-054	1	BE11965_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	CTTTAATTACAGCGTTTTTATTGTTGT	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11965_Vgib_Wil2025_COI	GLTB-054	1	BE11965_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	CCCCTCTTTACAAGTTCTTTAGGGT	151.26444
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11966_Asen_Wil2025_COI	GLTB-055	1	BE11966_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	GTTGTTGTTACAACACATGCTCTTTTG	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11966_Cjap_Sim2018_COI	GLTB-055	1	BE11966_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11966_Esin_And2018_Cyb	GLTB-055	1	BE11966_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-055	Cytb	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	ACCCCTCCTCATATCCAACCA	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11966_Mgig_Bot2012_COI	GLTB-055	1	BE11966_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	TCTTATTCGTTGGAGACTTTATAACCCT	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11966_Mjap_Gib2022_COI	GLTB-055	1	BE11966_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	AATTTAATTGTGACTAGGCATGGTCTG	151.26628
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11967_Cmae_Bot2011_COI	GLTB-056	1	BE11967_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	ATGAACAGTCTATCCTCCTTTAG	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11967_Hsan_Wil2021_COI	GLTB-056	1	BE11967_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	CCTGGGCCGGTATAGTAGGT	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11967_Mros_Gib2022_COI	GLTB-056	1	BE11967_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11967_Msal_Bot2012_COI	GLTB-056	1	BE11967_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	GYTAGTTCCRATGATGTTAGCTG	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11967_Mstr_Wil2021_COI	GLTB-056	1	BE11967_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	GTAGTGGTAACTACTCATGCTTTA	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11967_Pvir_Dia2013_COI	GLTB-056	1	BE11967_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	CTTAGTGGCATTAATTCGDAATCC	151.26783
BE11967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83093	WGS84	BE11967	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11967_Rhar_Sim2018_COI	GLTB-056	1	BE11967_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11967	Sample	BE11967	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-056	COI	45	BE11967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-056	CTGCTGCTATTGCTCATG	151.26783
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11969_Aamu_Bax2006_COI	GLTB-058	1	BE11969_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	GCACAACCGGGATCTTTACTTC	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11969_Pcan_Bot2011_IGS	GLTB-058	1	BE11969_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-058	IGS	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	CGTAATCCTCAGTACTGGCTA	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11969_Pper_Dia2013_COI	GLTB-058	1	BE11969_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	CTTAGTGGCATTAATTCGDAATCC	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11969_Sspa_Oph2007_COI	GLTB-058	1	BE11969_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	GAAGGCCTTCCGATGATC	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11969_Upin_Bot2011_COI	GLTB-058	1	BE11969_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	CTTTAATTACAGCGTTTTTATTGTTGT	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11969_Vgib_Wil2025_COI	GLTB-058	1	BE11969_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	CCCCTCTTTACAAGTTCTTTAGGGT	151.25955
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11970_Cmae_Bot2011_COI	GLTB-059	1	BE11970_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	ATGAACAGTCTATCCTCCTTTAG	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11970_Hsan_Wil2021_COI	GLTB-059	1	BE11970_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	CCTGGGCCGGTATAGTAGGT	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11970_Mros_Gib2022_COI	GLTB-059	1	BE11970_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11970_Msal_Bot2012_COI	GLTB-059	1	BE11970_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	GYTAGTTCCRATGATGTTAGCTG	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11970_Mstr_Wil2021_COI	GLTB-059	1	BE11970_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	GTAGTGGTAACTACTCATGCTTTA	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11970_Pvir_Dia2013_COI	GLTB-059	1	BE11970_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	CTTAGTGGCATTAATTCGDAATCC	151.26152
BE11970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83061	WGS84	BE11970	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11970_Rhar_Sim2018_COI	GLTB-059	1	BE11970_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11970	Sample	BE11970	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-059	COI	45	BE11970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-059	CTGCTGCTATTGCTCATG	151.26152
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11972_Aamu_Bax2006_COI	GLTB-061	1	BE11972_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	GCACAACCGGGATCTTTACTTC	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11972_Pcan_Bot2011_IGS	GLTB-061	1	BE11972_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-061	IGS	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	CGTAATCCTCAGTACTGGCTA	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11972_Pper_Dia2013_COI	GLTB-061	1	BE11972_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	CTTAGTGGCATTAATTCGDAATCC	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11972_Sspa_Oph2007_COI	GLTB-061	1	BE11972_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	GAAGGCCTTCCGATGATC	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11972_Upin_Bot2011_COI	GLTB-061	1	BE11972_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	CTTTAATTACAGCGTTTTTATTGTTGT	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11972_Vgib_Wil2025_COI	GLTB-061	1	BE11972_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	CCCCTCTTTACAAGTTCTTTAGGGT	151.26491
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11973_Asen_Wil2025_COI	GLTB-062	1	BE11973_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	GTTGTTGTTACAACACATGCTCTTTTG	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11973_Cjap_Sim2018_COI	GLTB-062	1	BE11973_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11973_Esin_And2018_Cyb	GLTB-062	1	BE11973_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-062	Cytb	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	ACCCCTCCTCATATCCAACCA	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11973_Mgig_Bot2012_COI	GLTB-062	1	BE11973_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	TCTTATTCGTTGGAGACTTTATAACCCT	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11973_Mjap_Gib2022_COI	GLTB-062	1	BE11973_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	AATTTAATTGTGACTAGGCATGGTCTG	151.25297
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11974_Cmae_Bot2011_COI	GLTB-063	1	BE11974_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	ATGAACAGTCTATCCTCCTTTAG	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11974_Hsan_Wil2021_COI	GLTB-063	1	BE11974_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	CCTGGGCCGGTATAGTAGGT	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11974_Mros_Gib2022_COI	GLTB-063	1	BE11974_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11974_Msal_Bot2012_COI	GLTB-063	1	BE11974_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	GYTAGTTCCRATGATGTTAGCTG	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11974_Mstr_Wil2021_COI	GLTB-063	1	BE11974_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	GTAGTGGTAACTACTCATGCTTTA	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11974_Pvir_Dia2013_COI	GLTB-063	1	BE11974_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	CTTAGTGGCATTAATTCGDAATCC	151.25482
BE11974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8296	WGS84	BE11974	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11974_Rhar_Sim2018_COI	GLTB-063	1	BE11974_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11974	Sample	BE11974	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-063	COI	45	BE11974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-063	CTGCTGCTATTGCTCATG	151.25482
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11976_Aamu_Bax2006_COI	GLTB-065	1	BE11976_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	GCACAACCGGGATCTTTACTTC	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11976_Pcan_Bot2011_IGS	GLTB-065	1	BE11976_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-065	IGS	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	CGTAATCCTCAGTACTGGCTA	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11976_Pper_Dia2013_COI	GLTB-065	1	BE11976_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	CTTAGTGGCATTAATTCGDAATCC	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11976_Sspa_Oph2007_COI	GLTB-065	1	BE11976_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	GAAGGCCTTCCGATGATC	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11976_Upin_Bot2011_COI	GLTB-065	1	BE11976_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	CTTTAATTACAGCGTTTTTATTGTTGT	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11976_Vgib_Wil2025_COI	GLTB-065	1	BE11976_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	CCCCTCTTTACAAGTTCTTTAGGGT	151.25842
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11977_Asen_Wil2025_COI	GLTB-066	1	BE11977_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	GTTGTTGTTACAACACATGCTCTTTTG	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11977_Cjap_Sim2018_COI	GLTB-066	1	BE11977_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11977_Esin_And2018_Cyb	GLTB-066	1	BE11977_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-066	Cytb	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	ACCCCTCCTCATATCCAACCA	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11977_Mgig_Bot2012_COI	GLTB-066	1	BE11977_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	TCTTATTCGTTGGAGACTTTATAACCCT	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11977_Mjap_Gib2022_COI	GLTB-066	1	BE11977_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	AATTTAATTGTGACTAGGCATGGTCTG	151.2599
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11978_Cmae_Bot2011_COI	GLTB-067	1	BE11978_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	ATGAACAGTCTATCCTCCTTTAG	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11978_Hsan_Wil2021_COI	GLTB-067	1	BE11978_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	CCTGGGCCGGTATAGTAGGT	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11978_Mros_Gib2022_COI	GLTB-067	1	BE11978_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11978_Msal_Bot2012_COI	GLTB-067	1	BE11978_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	GYTAGTTCCRATGATGTTAGCTG	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11978_Mstr_Wil2021_COI	GLTB-067	1	BE11978_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	GTAGTGGTAACTACTCATGCTTTA	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11978_Pvir_Dia2013_COI	GLTB-067	1	BE11978_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	CTTAGTGGCATTAATTCGDAATCC	151.26178
BE11978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82879	WGS84	BE11978	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11978_Rhar_Sim2018_COI	GLTB-067	1	BE11978_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11978	Sample	BE11978	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-067	COI	45	BE11978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-067	CTGCTGCTATTGCTCATG	151.26178
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11980_Asen_Wil2025_COI	GLTB-069	1	BE11980_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	GTTGTTGTTACAACACATGCTCTTTTG	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11980_Cjap_Sim2018_COI	GLTB-069	1	BE11980_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11980_Esin_And2018_Cyb	GLTB-069	1	BE11980_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-069	Cytb	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	ACCCCTCCTCATATCCAACCA	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11980_Mgig_Bot2012_COI	GLTB-069	1	BE11980_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	TCTTATTCGTTGGAGACTTTATAACCCT	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11980_Mjap_Gib2022_COI	GLTB-069	1	BE11980_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	AATTTAATTGTGACTAGGCATGGTCTG	151.25566
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11981_Cmae_Bot2011_COI	GLTB-070	1	BE11981_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	ATGAACAGTCTATCCTCCTTTAG	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11981_Hsan_Wil2021_COI	GLTB-070	1	BE11981_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	CCTGGGCCGGTATAGTAGGT	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11981_Mros_Gib2022_COI	GLTB-070	1	BE11981_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11981_Msal_Bot2012_COI	GLTB-070	1	BE11981_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	GYTAGTTCCRATGATGTTAGCTG	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11981_Mstr_Wil2021_COI	GLTB-070	1	BE11981_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	GTAGTGGTAACTACTCATGCTTTA	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11981_Pvir_Dia2013_COI	GLTB-070	1	BE11981_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	CTTAGTGGCATTAATTCGDAATCC	151.2573
BE11981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82787	WGS84	BE11981	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11981_Rhar_Sim2018_COI	GLTB-070	1	BE11981_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11981	Sample	BE11981	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-070	COI	45	BE11981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-070	CTGCTGCTATTGCTCATG	151.2573
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11983_Aamu_Bax2006_COI	GLTB-037	1	BE11983_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	GCACAACCGGGATCTTTACTTC	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11983_Pcan_Bot2011_IGS	GLTB-037	1	BE11983_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-037	IGS	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	CGTAATCCTCAGTACTGGCTA	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11983_Pper_Dia2013_COI	GLTB-037	1	BE11983_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	CTTAGTGGCATTAATTCGDAATCC	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11983_Sspa_Oph2007_COI	GLTB-037	1	BE11983_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	GAAGGCCTTCCGATGATC	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11983_Upin_Bot2011_COI	GLTB-037	1	BE11983_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	CTTTAATTACAGCGTTTTTATTGTTGT	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11983_Vgib_Wil2025_COI	GLTB-037	1	BE11983_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	CCCCTCTTTACAAGTTCTTTAGGGT	151.27454
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11984_Asen_Wil2025_COI	GLTB-038	1	BE11984_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	GTTGTTGTTACAACACATGCTCTTTTG	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11984_Cjap_Sim2018_COI	GLTB-038	1	BE11984_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11984_Esin_And2018_Cyb	GLTB-038	1	BE11984_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-038	Cytb	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	ACCCCTCCTCATATCCAACCA	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11984_Mgig_Bot2012_COI	GLTB-038	1	BE11984_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	TCTTATTCGTTGGAGACTTTATAACCCT	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11984_Mjap_Gib2022_COI	GLTB-038	1	BE11984_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	AATTTAATTGTGACTAGGCATGGTCTG	151.27761
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11985_Cmae_Bot2011_COI	GLTB-039	1	BE11985_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	ATGAACAGTCTATCCTCCTTTAG	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11985_Hsan_Wil2021_COI	GLTB-039	1	BE11985_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	CCTGGGCCGGTATAGTAGGT	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11985_Mros_Gib2022_COI	GLTB-039	1	BE11985_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11985_Msal_Bot2012_COI	GLTB-039	1	BE11985_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	GYTAGTTCCRATGATGTTAGCTG	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11985_Mstr_Wil2021_COI	GLTB-039	1	BE11985_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	GTAGTGGTAACTACTCATGCTTTA	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11985_Pvir_Dia2013_COI	GLTB-039	1	BE11985_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	CTTAGTGGCATTAATTCGDAATCC	151.27637
BE11985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83579	WGS84	BE11985	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11985_Rhar_Sim2018_COI	GLTB-039	1	BE11985_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11985	Sample	BE11985	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-039	COI	45	BE11985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-039	CTGCTGCTATTGCTCATG	151.27637
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11991_Asen_Wil2025_COI	GLTB-071	1	BE11991_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	GTTGTTGTTACAACACATGCTCTTTTG	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11991_Cjap_Sim2018_COI	GLTB-071	1	BE11991_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11991_Esin_And2018_Cyb	GLTB-071	1	BE11991_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-071	Cytb	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	ACCCCTCCTCATATCCAACCA	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11991_Mgig_Bot2012_COI	GLTB-071	1	BE11991_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	TCTTATTCGTTGGAGACTTTATAACCCT	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11991_Mjap_Gib2022_COI	GLTB-071	1	BE11991_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	AATTTAATTGTGACTAGGCATGGTCTG	151.18361
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11992_Cmae_Bot2011_COI	GLTB-072	1	BE11992_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	ATGAACAGTCTATCCTCCTTTAG	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11992_Hsan_Wil2021_COI	GLTB-072	1	BE11992_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	CCTGGGCCGGTATAGTAGGT	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11992_Mros_Gib2022_COI	GLTB-072	1	BE11992_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11992_Msal_Bot2012_COI	GLTB-072	1	BE11992_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	GYTAGTTCCRATGATGTTAGCTG	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11992_Mstr_Wil2021_COI	GLTB-072	1	BE11992_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	GTAGTGGTAACTACTCATGCTTTA	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11992_Pvir_Dia2013_COI	GLTB-072	1	BE11992_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	CTTAGTGGCATTAATTCGDAATCC	151.1841
BE11992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79442	WGS84	BE11992	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11992_Rhar_Sim2018_COI	GLTB-072	1	BE11992_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11992	Sample	BE11992	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-072	COI	45	BE11992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-072	CTGCTGCTATTGCTCATG	151.1841
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11994_Aamu_Bax2006_COI	GLTB-074	1	BE11994_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	GCACAACCGGGATCTTTACTTC	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11994_Pcan_Bot2011_IGS	GLTB-074	1	BE11994_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-074	IGS	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	CGTAATCCTCAGTACTGGCTA	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11994_Pper_Dia2013_COI	GLTB-074	1	BE11994_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	CTTAGTGGCATTAATTCGDAATCC	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11994_Sspa_Oph2007_COI	GLTB-074	1	BE11994_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	GAAGGCCTTCCGATGATC	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11994_Upin_Bot2011_COI	GLTB-074	1	BE11994_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	CTTTAATTACAGCGTTTTTATTGTTGT	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11994_Vgib_Wil2025_COI	GLTB-074	1	BE11994_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	CCCCTCTTTACAAGTTCTTTAGGGT	151.18773
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11995_Asen_Wil2025_COI	GLTB-075	1	BE11995_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	GTTGTTGTTACAACACATGCTCTTTTG	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11995_Cjap_Sim2018_COI	GLTB-075	1	BE11995_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11995_Esin_And2018_Cyb	GLTB-075	1	BE11995_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-075	Cytb	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	ACCCCTCCTCATATCCAACCA	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11995_Mgig_Bot2012_COI	GLTB-075	1	BE11995_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	TCTTATTCGTTGGAGACTTTATAACCCT	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11995_Mjap_Gib2022_COI	GLTB-075	1	BE11995_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	AATTTAATTGTGACTAGGCATGGTCTG	151.18169
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11996_Cmae_Bot2011_COI	GLTB-076	1	BE11996_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	ATGAACAGTCTATCCTCCTTTAG	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11996_Hsan_Wil2021_COI	GLTB-076	1	BE11996_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	CCTGGGCCGGTATAGTAGGT	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11996_Mros_Gib2022_COI	GLTB-076	1	BE11996_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11996_Msal_Bot2012_COI	GLTB-076	1	BE11996_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	GYTAGTTCCRATGATGTTAGCTG	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11996_Mstr_Wil2021_COI	GLTB-076	1	BE11996_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	GTAGTGGTAACTACTCATGCTTTA	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11996_Pvir_Dia2013_COI	GLTB-076	1	BE11996_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	CTTAGTGGCATTAATTCGDAATCC	151.18546
BE11996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79294	WGS84	BE11996	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11996_Rhar_Sim2018_COI	GLTB-076	1	BE11996_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11996	Sample	BE11996	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-076	COI	45	BE11996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-076	CTGCTGCTATTGCTCATG	151.18546
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11998_Aamu_Bax2006_COI	GLTB-078	1	BE11998_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	GCACAACCGGGATCTTTACTTC	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11998_Pcan_Bot2011_IGS	GLTB-078	1	BE11998_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-078	IGS	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	CGTAATCCTCAGTACTGGCTA	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11998_Pper_Dia2013_COI	GLTB-078	1	BE11998_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	CTTAGTGGCATTAATTCGDAATCC	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11998_Sspa_Oph2007_COI	GLTB-078	1	BE11998_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	GAAGGCCTTCCGATGATC	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11998_Upin_Bot2011_COI	GLTB-078	1	BE11998_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	CTTTAATTACAGCGTTTTTATTGTTGT	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11998_Vgib_Wil2025_COI	GLTB-078	1	BE11998_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	CCCCTCTTTACAAGTTCTTTAGGGT	151.18853
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11999_Asen_Wil2025_COI	GLTB-079	1	BE11999_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	GTTGTTGTTACAACACATGCTCTTTTG	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11999_Cjap_Sim2018_COI	GLTB-079	1	BE11999_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11999_Esin_And2018_Cyb	GLTB-079	1	BE11999_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-079	Cytb	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	ACCCCTCCTCATATCCAACCA	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11999_Mgig_Bot2012_COI	GLTB-079	1	BE11999_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	TCTTATTCGTTGGAGACTTTATAACCCT	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11999_Mjap_Gib2022_COI	GLTB-079	1	BE11999_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	AATTTAATTGTGACTAGGCATGGTCTG	151.18068
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12001_Aamu_Bax2006_COI	GLTB-081	1	BE12001_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	GCACAACCGGGATCTTTACTTC	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12001_Pcan_Bot2011_IGS	GLTB-081	1	BE12001_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-081	IGS	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	CGTAATCCTCAGTACTGGCTA	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12001_Pper_Dia2013_COI	GLTB-081	1	BE12001_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	CTTAGTGGCATTAATTCGDAATCC	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12001_Sspa_Oph2007_COI	GLTB-081	1	BE12001_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	GAAGGCCTTCCGATGATC	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12001_Upin_Bot2011_COI	GLTB-081	1	BE12001_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	CTTTAATTACAGCGTTTTTATTGTTGT	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12001_Vgib_Wil2025_COI	GLTB-081	1	BE12001_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	CCCCTCTTTACAAGTTCTTTAGGGT	151.1876
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12002_Asen_Wil2025_COI	GLTB-082	1	BE12002_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	GTTGTTGTTACAACACATGCTCTTTTG	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12002_Cjap_Sim2018_COI	GLTB-082	1	BE12002_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12002_Esin_And2018_Cyb	GLTB-082	1	BE12002_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-082	Cytb	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	ACCCCTCCTCATATCCAACCA	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12002_Mgig_Bot2012_COI	GLTB-082	1	BE12002_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	TCTTATTCGTTGGAGACTTTATAACCCT	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12002_Mjap_Gib2022_COI	GLTB-082	1	BE12002_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	AATTTAATTGTGACTAGGCATGGTCTG	151.18299
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12003_Cmae_Bot2011_COI	GLTB-083	1	BE12003_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	ATGAACAGTCTATCCTCCTTTAG	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12003_Hsan_Wil2021_COI	GLTB-083	1	BE12003_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	CCTGGGCCGGTATAGTAGGT	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12003_Mros_Gib2022_COI	GLTB-083	1	BE12003_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12003_Msal_Bot2012_COI	GLTB-083	1	BE12003_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	GYTAGTTCCRATGATGTTAGCTG	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12003_Mstr_Wil2021_COI	GLTB-083	1	BE12003_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	GTAGTGGTAACTACTCATGCTTTA	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12003_Pvir_Dia2013_COI	GLTB-083	1	BE12003_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	CTTAGTGGCATTAATTCGDAATCC	151.18459
BE12003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79021	WGS84	BE12003	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12003_Rhar_Sim2018_COI	GLTB-083	1	BE12003_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12003	Sample	BE12003	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-083	COI	45	BE12003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-083	CTGCTGCTATTGCTCATG	151.18459
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12005_Aamu_Bax2006_COI	GLTB-085	1	BE12005_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	GCACAACCGGGATCTTTACTTC	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12005_Pcan_Bot2011_IGS	GLTB-085	1	BE12005_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-085	IGS	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	CGTAATCCTCAGTACTGGCTA	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12005_Pper_Dia2013_COI	GLTB-085	1	BE12005_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	CTTAGTGGCATTAATTCGDAATCC	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12005_Sspa_Oph2007_COI	GLTB-085	1	BE12005_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	GAAGGCCTTCCGATGATC	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12005_Upin_Bot2011_COI	GLTB-085	1	BE12005_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	CTTTAATTACAGCGTTTTTATTGTTGT	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12005_Vgib_Wil2025_COI	GLTB-085	1	BE12005_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	CCCCTCTTTACAAGTTCTTTAGGGT	151.18209
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12006_Asen_Wil2025_COI	GLTB-086	1	BE12006_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	GTTGTTGTTACAACACATGCTCTTTTG	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12006_Cjap_Sim2018_COI	GLTB-086	1	BE12006_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12006_Esin_And2018_Cyb	GLTB-086	1	BE12006_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-086	Cytb	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	ACCCCTCCTCATATCCAACCA	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12006_Mgig_Bot2012_COI	GLTB-086	1	BE12006_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	TCTTATTCGTTGGAGACTTTATAACCCT	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12006_Mjap_Gib2022_COI	GLTB-086	1	BE12006_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	AATTTAATTGTGACTAGGCATGGTCTG	151.18354
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12007_Cmae_Bot2011_COI	GLTB-087	1	BE12007_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	ATGAACAGTCTATCCTCCTTTAG	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12007_Hsan_Wil2021_COI	GLTB-087	1	BE12007_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	CCTGGGCCGGTATAGTAGGT	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12007_Mros_Gib2022_COI	GLTB-087	1	BE12007_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12007_Msal_Bot2012_COI	GLTB-087	1	BE12007_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	GYTAGTTCCRATGATGTTAGCTG	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12007_Mstr_Wil2021_COI	GLTB-087	1	BE12007_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	GTAGTGGTAACTACTCATGCTTTA	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12007_Pvir_Dia2013_COI	GLTB-087	1	BE12007_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	CTTAGTGGCATTAATTCGDAATCC	151.18521
BE12007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78874	WGS84	BE12007	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12007_Rhar_Sim2018_COI	GLTB-087	1	BE12007_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12007	Sample	BE12007	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-087	COI	45	BE12007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-087	CTGCTGCTATTGCTCATG	151.18521
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12009_Aamu_Bax2006_COI	GLTB-089	1	BE12009_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	GCACAACCGGGATCTTTACTTC	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12009_Pcan_Bot2011_IGS	GLTB-089	1	BE12009_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-089	IGS	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	CGTAATCCTCAGTACTGGCTA	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12009_Pper_Dia2013_COI	GLTB-089	1	BE12009_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	CTTAGTGGCATTAATTCGDAATCC	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12009_Sspa_Oph2007_COI	GLTB-089	1	BE12009_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	GAAGGCCTTCCGATGATC	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12009_Upin_Bot2011_COI	GLTB-089	1	BE12009_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	CTTTAATTACAGCGTTTTTATTGTTGT	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12009_Vgib_Wil2025_COI	GLTB-089	1	BE12009_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	CCCCTCTTTACAAGTTCTTTAGGGT	151.18263
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12010_Cmae_Bot2011_COI	GLTB-090	1	BE12010_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	ATGAACAGTCTATCCTCCTTTAG	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12010_Hsan_Wil2021_COI	GLTB-090	1	BE12010_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	CCTGGGCCGGTATAGTAGGT	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12010_Mros_Gib2022_COI	GLTB-090	1	BE12010_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12010_Msal_Bot2012_COI	GLTB-090	1	BE12010_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	GYTAGTTCCRATGATGTTAGCTG	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12010_Mstr_Wil2021_COI	GLTB-090	1	BE12010_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	GTAGTGGTAACTACTCATGCTTTA	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12010_Pvir_Dia2013_COI	GLTB-090	1	BE12010_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	CTTAGTGGCATTAATTCGDAATCC	151.1843
BE12010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78777	WGS84	BE12010	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12010_Rhar_Sim2018_COI	GLTB-090	1	BE12010_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12010	Sample	BE12010	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-090	COI	45	BE12010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-090	CTGCTGCTATTGCTCATG	151.1843
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12012_Aamu_Bax2006_COI	GLTB-092	1	BE12012_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	GCACAACCGGGATCTTTACTTC	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12012_Pcan_Bot2011_IGS	GLTB-092	1	BE12012_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-092	IGS	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	CGTAATCCTCAGTACTGGCTA	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12012_Pper_Dia2013_COI	GLTB-092	1	BE12012_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	CTTAGTGGCATTAATTCGDAATCC	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12012_Sspa_Oph2007_COI	GLTB-092	1	BE12012_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	GAAGGCCTTCCGATGATC	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12012_Upin_Bot2011_COI	GLTB-092	1	BE12012_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	CTTTAATTACAGCGTTTTTATTGTTGT	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12012_Vgib_Wil2025_COI	GLTB-092	1	BE12012_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	CCCCTCTTTACAAGTTCTTTAGGGT	151.17995
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12013_Asen_Wil2025_COI	GLTB-093	1	BE12013_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	GTTGTTGTTACAACACATGCTCTTTTG	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12013_Cjap_Sim2018_COI	GLTB-093	1	BE12013_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12013_Esin_And2018_Cyb	GLTB-093	1	BE12013_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-093	Cytb	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	ACCCCTCCTCATATCCAACCA	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12013_Mgig_Bot2012_COI	GLTB-093	1	BE12013_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	TCTTATTCGTTGGAGACTTTATAACCCT	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12013_Mjap_Gib2022_COI	GLTB-093	1	BE12013_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	AATTTAATTGTGACTAGGCATGGTCTG	151.18144
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12014_Cmae_Bot2011_COI	GLTB-094	1	BE12014_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	ATGAACAGTCTATCCTCCTTTAG	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12014_Hsan_Wil2021_COI	GLTB-094	1	BE12014_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	CCTGGGCCGGTATAGTAGGT	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12014_Mros_Gib2022_COI	GLTB-094	1	BE12014_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12014_Msal_Bot2012_COI	GLTB-094	1	BE12014_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	GYTAGTTCCRATGATGTTAGCTG	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12014_Mstr_Wil2021_COI	GLTB-094	1	BE12014_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	GTAGTGGTAACTACTCATGCTTTA	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12014_Pvir_Dia2013_COI	GLTB-094	1	BE12014_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	CTTAGTGGCATTAATTCGDAATCC	151.17722
BE12014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78618	WGS84	BE12014	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12014_Rhar_Sim2018_COI	GLTB-094	1	BE12014_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12014	Sample	BE12014	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-094	COI	45	BE12014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-094	CTGCTGCTATTGCTCATG	151.17722
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12016_Aamu_Bax2006_COI	GLTB-096	1	BE12016_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	GCACAACCGGGATCTTTACTTC	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12016_Pcan_Bot2011_IGS	GLTB-096	1	BE12016_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-096	IGS	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	CGTAATCCTCAGTACTGGCTA	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12016_Pper_Dia2013_COI	GLTB-096	1	BE12016_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	CTTAGTGGCATTAATTCGDAATCC	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12016_Sspa_Oph2007_COI	GLTB-096	1	BE12016_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	GAAGGCCTTCCGATGATC	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12016_Upin_Bot2011_COI	GLTB-096	1	BE12016_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	CTTTAATTACAGCGTTTTTATTGTTGT	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12016_Vgib_Wil2025_COI	GLTB-096	1	BE12016_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	CCCCTCTTTACAAGTTCTTTAGGGT	151.18077
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12017_Asen_Wil2025_COI	GLTB-097	1	BE12017_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	GTTGTTGTTACAACACATGCTCTTTTG	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12017_Cjap_Sim2018_COI	GLTB-097	1	BE12017_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12017_Esin_And2018_Cyb	GLTB-097	1	BE12017_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-097	Cytb	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	ACCCCTCCTCATATCCAACCA	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12017_Mgig_Bot2012_COI	GLTB-097	1	BE12017_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	TCTTATTCGTTGGAGACTTTATAACCCT	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12017_Mjap_Gib2022_COI	GLTB-097	1	BE12017_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	AATTTAATTGTGACTAGGCATGGTCTG	151.17635
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12018_Cmae_Bot2011_COI	GLTB-098	1	BE12018_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	ATGAACAGTCTATCCTCCTTTAG	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12018_Hsan_Wil2021_COI	GLTB-098	1	BE12018_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	CCTGGGCCGGTATAGTAGGT	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12018_Mros_Gib2022_COI	GLTB-098	1	BE12018_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12018_Msal_Bot2012_COI	GLTB-098	1	BE12018_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	GYTAGTTCCRATGATGTTAGCTG	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12018_Mstr_Wil2021_COI	GLTB-098	1	BE12018_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	GTAGTGGTAACTACTCATGCTTTA	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12018_Pvir_Dia2013_COI	GLTB-098	1	BE12018_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	CTTAGTGGCATTAATTCGDAATCC	151.17811
BE12018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78435	WGS84	BE12018	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12018_Rhar_Sim2018_COI	GLTB-098	1	BE12018_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12018	Sample	BE12018	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-098	COI	45	BE12018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-098	CTGCTGCTATTGCTCATG	151.17811
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12020_Asen_Wil2025_COI	GLTB-100	1	BE12020_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	GTTGTTGTTACAACACATGCTCTTTTG	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12020_Cjap_Sim2018_COI	GLTB-100	1	BE12020_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12020_Esin_And2018_Cyb	GLTB-100	1	BE12020_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-100	Cytb	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	ACCCCTCCTCATATCCAACCA	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12020_Mgig_Bot2012_COI	GLTB-100	1	BE12020_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	TCTTATTCGTTGGAGACTTTATAACCCT	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12020_Mjap_Gib2022_COI	GLTB-100	1	BE12020_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	AATTTAATTGTGACTAGGCATGGTCTG	151.17533
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12021_Cmae_Bot2011_COI	GLTB-101	1	BE12021_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	ATGAACAGTCTATCCTCCTTTAG	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12021_Hsan_Wil2021_COI	GLTB-101	1	BE12021_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	CCTGGGCCGGTATAGTAGGT	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12021_Mros_Gib2022_COI	GLTB-101	1	BE12021_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12021_Msal_Bot2012_COI	GLTB-101	1	BE12021_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	GYTAGTTCCRATGATGTTAGCTG	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12021_Mstr_Wil2021_COI	GLTB-101	1	BE12021_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	GTAGTGGTAACTACTCATGCTTTA	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12021_Pvir_Dia2013_COI	GLTB-101	1	BE12021_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	CTTAGTGGCATTAATTCGDAATCC	151.17671
BE12021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78321	WGS84	BE12021	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12021_Rhar_Sim2018_COI	GLTB-101	1	BE12021_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12021	Sample	BE12021	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-101	COI	45	BE12021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-101	CTGCTGCTATTGCTCATG	151.17671
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12023_Aamu_Bax2006_COI	GLTB-103	1	BE12023_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	GCACAACCGGGATCTTTACTTC	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12023_Pcan_Bot2011_IGS	GLTB-103	1	BE12023_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-103	IGS	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	CGTAATCCTCAGTACTGGCTA	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12023_Pper_Dia2013_COI	GLTB-103	1	BE12023_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	CTTAGTGGCATTAATTCGDAATCC	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12023_Sspa_Oph2007_COI	GLTB-103	1	BE12023_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	GAAGGCCTTCCGATGATC	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12023_Upin_Bot2011_COI	GLTB-103	1	BE12023_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	CTTTAATTACAGCGTTTTTATTGTTGT	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12023_Vgib_Wil2025_COI	GLTB-103	1	BE12023_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	CCCCTCTTTACAAGTTCTTTAGGGT	151.17423
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12024_Asen_Wil2025_COI	GLTB-104	1	BE12024_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	GTTGTTGTTACAACACATGCTCTTTTG	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12024_Cjap_Sim2018_COI	GLTB-104	1	BE12024_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12024_Esin_And2018_Cyb	GLTB-104	1	BE12024_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-104	Cytb	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	ACCCCTCCTCATATCCAACCA	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12024_Mgig_Bot2012_COI	GLTB-104	1	BE12024_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	TCTTATTCGTTGGAGACTTTATAACCCT	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12024_Mjap_Gib2022_COI	GLTB-104	1	BE12024_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	AATTTAATTGTGACTAGGCATGGTCTG	151.17575
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12025_Cmae_Bot2011_COI	GLTB-105	1	BE12025_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	ATGAACAGTCTATCCTCCTTTAG	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12025_Hsan_Wil2021_COI	GLTB-105	1	BE12025_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	CCTGGGCCGGTATAGTAGGT	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12025_Mros_Gib2022_COI	GLTB-105	1	BE12025_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12025_Msal_Bot2012_COI	GLTB-105	1	BE12025_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	GYTAGTTCCRATGATGTTAGCTG	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12025_Mstr_Wil2021_COI	GLTB-105	1	BE12025_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	GTAGTGGTAACTACTCATGCTTTA	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12025_Pvir_Dia2013_COI	GLTB-105	1	BE12025_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	CTTAGTGGCATTAATTCGDAATCC	151.17319
BE12025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78108	WGS84	BE12025	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12025_Rhar_Sim2018_COI	GLTB-105	1	BE12025_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12025	Sample	BE12025	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-105	COI	45	BE12025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-105	CTGCTGCTATTGCTCATG	151.17319
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32692_Aamu_Bax2006_COI	CNSB-001	1	BE32692_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	GCACAACCGGGATCTTTACTTC	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32692_Pcan_Bot2011_IGS	CNSB-001	1	BE32692_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-001	IGS	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	CGTAATCCTCAGTACTGGCTA	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32692_Pper_Dia2013_COI	CNSB-001	1	BE32692_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	CTTAGTGGCATTAATTCGDAATCC	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32692_Sspa_Oph2007_COI	CNSB-001	1	BE32692_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	GAAGGCCTTCCGATGATC	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32692_Upin_Bot2011_COI	CNSB-001	1	BE32692_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	CTTTAATTACAGCGTTTTTATTGTTGT	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32692_Vgib_Wil2025_COI	CNSB-001	1	BE32692_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	CCCCTCTTTACAAGTTCTTTAGGGT	145.77391
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32693_Asen_Wil2025_COI	CNSB-002	1	BE32693_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	GTTGTTGTTACAACACATGCTCTTTTG	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32693_Cjap_Sim2018_COI	CNSB-002	1	BE32693_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32693_Esin_And2018_Cyb	CNSB-002	1	BE32693_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-002	Cytb	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	ACCCCTCCTCATATCCAACCA	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32693_Mgig_Bot2012_COI	CNSB-002	1	BE32693_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	TCTTATTCGTTGGAGACTTTATAACCCT	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32693_Mjap_Gib2022_COI	CNSB-002	1	BE32693_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	AATTTAATTGTGACTAGGCATGGTCTG	145.77343
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32694_Cmae_Bot2011_COI	CNSB-003	1	BE32694_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	ATGAACAGTCTATCCTCCTTTAG	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32694_Hsan_Wil2021_COI	CNSB-003	1	BE32694_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	CCTGGGCCGGTATAGTAGGT	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32694_Mros_Gib2022_COI	CNSB-003	1	BE32694_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32694_Msal_Bot2012_COI	CNSB-003	1	BE32694_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	GYTAGTTCCRATGATGTTAGCTG	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32694_Mstr_Wil2021_COI	CNSB-003	1	BE32694_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	GTAGTGGTAACTACTCATGCTTTA	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32694_Pvir_Dia2013_COI	CNSB-003	1	BE32694_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	CTTAGTGGCATTAATTCGDAATCC	145.7747
BE32694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94609	WGS84	BE32694	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32694_Rhar_Sim2018_COI	CNSB-003	1	BE32694_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32694	Sample	BE32694	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-003	COI	45	BE32694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-003	CTGCTGCTATTGCTCATG	145.7747
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32696_Aamu_Bax2006_COI	CNSB-005	1	BE32696_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	GCACAACCGGGATCTTTACTTC	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32696_Pcan_Bot2011_IGS	CNSB-005	1	BE32696_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-005	IGS	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	CGTAATCCTCAGTACTGGCTA	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32696_Pper_Dia2013_COI	CNSB-005	1	BE32696_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	CTTAGTGGCATTAATTCGDAATCC	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32696_Sspa_Oph2007_COI	CNSB-005	1	BE32696_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	GAAGGCCTTCCGATGATC	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32696_Upin_Bot2011_COI	CNSB-005	1	BE32696_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	CTTTAATTACAGCGTTTTTATTGTTGT	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32696_Vgib_Wil2025_COI	CNSB-005	1	BE32696_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	CCCCTCTTTACAAGTTCTTTAGGGT	145.77936
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32697_Asen_Wil2025_COI	CNSB-006	1	BE32697_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	GTTGTTGTTACAACACATGCTCTTTTG	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32697_Cjap_Sim2018_COI	CNSB-006	1	BE32697_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32697_Esin_And2018_Cyb	CNSB-006	1	BE32697_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-006	Cytb	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	ACCCCTCCTCATATCCAACCA	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32697_Mgig_Bot2012_COI	CNSB-006	1	BE32697_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	TCTTATTCGTTGGAGACTTTATAACCCT	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32697_Mjap_Gib2022_COI	CNSB-006	1	BE32697_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	AATTTAATTGTGACTAGGCATGGTCTG	145.7822
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32698_Cmae_Bot2011_COI	CNSB-007	1	BE32698_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	ATGAACAGTCTATCCTCCTTTAG	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32698_Hsan_Wil2021_COI	CNSB-007	1	BE32698_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	CCTGGGCCGGTATAGTAGGT	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32698_Mros_Gib2022_COI	CNSB-007	1	BE32698_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32698_Msal_Bot2012_COI	CNSB-007	1	BE32698_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	GYTAGTTCCRATGATGTTAGCTG	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32698_Mstr_Wil2021_COI	CNSB-007	1	BE32698_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	GTAGTGGTAACTACTCATGCTTTA	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32698_Pvir_Dia2013_COI	CNSB-007	1	BE32698_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	CTTAGTGGCATTAATTCGDAATCC	145.78164
BE32698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94109	WGS84	BE32698	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32698_Rhar_Sim2018_COI	CNSB-007	1	BE32698_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32698	Sample	BE32698	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-007	COI	45	BE32698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-007	CTGCTGCTATTGCTCATG	145.78164
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32700_Aamu_Bax2006_COI	CNSB-009	1	BE32700_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	GCACAACCGGGATCTTTACTTC	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32700_Pcan_Bot2011_IGS	CNSB-009	1	BE32700_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-009	IGS	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	CGTAATCCTCAGTACTGGCTA	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32700_Pper_Dia2013_COI	CNSB-009	1	BE32700_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	CTTAGTGGCATTAATTCGDAATCC	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32700_Sspa_Oph2007_COI	CNSB-009	1	BE32700_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	GAAGGCCTTCCGATGATC	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32700_Upin_Bot2011_COI	CNSB-009	1	BE32700_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	CTTTAATTACAGCGTTTTTATTGTTGT	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32700_Vgib_Wil2025_COI	CNSB-009	1	BE32700_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	CCCCTCTTTACAAGTTCTTTAGGGT	145.78192
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32701_Asen_Wil2025_COI	CNSB-010	1	BE32701_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	GTTGTTGTTACAACACATGCTCTTTTG	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32701_Cjap_Sim2018_COI	CNSB-010	1	BE32701_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32701_Esin_And2018_Cyb	CNSB-010	1	BE32701_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-010	Cytb	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	ACCCCTCCTCATATCCAACCA	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32701_Mgig_Bot2012_COI	CNSB-010	1	BE32701_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	TCTTATTCGTTGGAGACTTTATAACCCT	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32701_Mjap_Gib2022_COI	CNSB-010	1	BE32701_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	AATTTAATTGTGACTAGGCATGGTCTG	145.78101
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32702_Cmae_Bot2011_COI	CNSB-011	1	BE32702_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	ATGAACAGTCTATCCTCCTTTAG	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32702_Hsan_Wil2021_COI	CNSB-011	1	BE32702_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	CCTGGGCCGGTATAGTAGGT	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32702_Mros_Gib2022_COI	CNSB-011	1	BE32702_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32702_Msal_Bot2012_COI	CNSB-011	1	BE32702_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	GYTAGTTCCRATGATGTTAGCTG	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32702_Mstr_Wil2021_COI	CNSB-011	1	BE32702_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	GTAGTGGTAACTACTCATGCTTTA	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32702_Pvir_Dia2013_COI	CNSB-011	1	BE32702_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	CTTAGTGGCATTAATTCGDAATCC	145.78247
BE32702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9375	WGS84	BE32702	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32702_Rhar_Sim2018_COI	CNSB-011	1	BE32702_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32702	Sample	BE32702	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-011	COI	45	BE32702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-011	CTGCTGCTATTGCTCATG	145.78247
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32704_Aamu_Bax2006_COI	CNSB-013	1	BE32704_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	GCACAACCGGGATCTTTACTTC	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32704_Pcan_Bot2011_IGS	CNSB-013	1	BE32704_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-013	IGS	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	CGTAATCCTCAGTACTGGCTA	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32704_Pper_Dia2013_COI	CNSB-013	1	BE32704_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	CTTAGTGGCATTAATTCGDAATCC	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32704_Sspa_Oph2007_COI	CNSB-013	1	BE32704_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	GAAGGCCTTCCGATGATC	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32704_Upin_Bot2011_COI	CNSB-013	1	BE32704_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	CTTTAATTACAGCGTTTTTATTGTTGT	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32704_Vgib_Wil2025_COI	CNSB-013	1	BE32704_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	CCCCTCTTTACAAGTTCTTTAGGGT	145.78072
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32705_Asen_Wil2025_COI	CNSB-014	1	BE32705_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	GTTGTTGTTACAACACATGCTCTTTTG	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32705_Cjap_Sim2018_COI	CNSB-014	1	BE32705_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32705_Esin_And2018_Cyb	CNSB-014	1	BE32705_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-014	Cytb	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	ACCCCTCCTCATATCCAACCA	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32705_Mgig_Bot2012_COI	CNSB-014	1	BE32705_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	TCTTATTCGTTGGAGACTTTATAACCCT	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32705_Mjap_Gib2022_COI	CNSB-014	1	BE32705_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	AATTTAATTGTGACTAGGCATGGTCTG	145.78204
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32706_Cmae_Bot2011_COI	CNSB-015	1	BE32706_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	ATGAACAGTCTATCCTCCTTTAG	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32706_Hsan_Wil2021_COI	CNSB-015	1	BE32706_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	CCTGGGCCGGTATAGTAGGT	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32706_Mros_Gib2022_COI	CNSB-015	1	BE32706_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32706_Msal_Bot2012_COI	CNSB-015	1	BE32706_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	GYTAGTTCCRATGATGTTAGCTG	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32706_Mstr_Wil2021_COI	CNSB-015	1	BE32706_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	GTAGTGGTAACTACTCATGCTTTA	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32706_Pvir_Dia2013_COI	CNSB-015	1	BE32706_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	CTTAGTGGCATTAATTCGDAATCC	145.78114
BE32706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9333	WGS84	BE32706	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32706_Rhar_Sim2018_COI	CNSB-015	1	BE32706_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32706	Sample	BE32706	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-015	COI	45	BE32706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-015	CTGCTGCTATTGCTCATG	145.78114
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32708_Aamu_Bax2006_COI	CNSB-017	1	BE32708_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	GCACAACCGGGATCTTTACTTC	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32708_Pcan_Bot2011_IGS	CNSB-017	1	BE32708_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-017	IGS	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	CGTAATCCTCAGTACTGGCTA	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32708_Pper_Dia2013_COI	CNSB-017	1	BE32708_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	CTTAGTGGCATTAATTCGDAATCC	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32708_Sspa_Oph2007_COI	CNSB-017	1	BE32708_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	GAAGGCCTTCCGATGATC	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32708_Upin_Bot2011_COI	CNSB-017	1	BE32708_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	CTTTAATTACAGCGTTTTTATTGTTGT	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32708_Vgib_Wil2025_COI	CNSB-017	1	BE32708_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	CCCCTCTTTACAAGTTCTTTAGGGT	145.7822
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32709_Asen_Wil2025_COI	CNSB-018	1	BE32709_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	GTTGTTGTTACAACACATGCTCTTTTG	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32709_Cjap_Sim2018_COI	CNSB-018	1	BE32709_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32709_Esin_And2018_Cyb	CNSB-018	1	BE32709_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-018	Cytb	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	ACCCCTCCTCATATCCAACCA	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32709_Mgig_Bot2012_COI	CNSB-018	1	BE32709_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	TCTTATTCGTTGGAGACTTTATAACCCT	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32709_Mjap_Gib2022_COI	CNSB-018	1	BE32709_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	AATTTAATTGTGACTAGGCATGGTCTG	145.78124
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32711_Aamu_Bax2006_COI	CNSB-020	1	BE32711_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	GCACAACCGGGATCTTTACTTC	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32711_Pcan_Bot2011_IGS	CNSB-020	1	BE32711_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-020	IGS	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	CGTAATCCTCAGTACTGGCTA	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32711_Pper_Dia2013_COI	CNSB-020	1	BE32711_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	CTTAGTGGCATTAATTCGDAATCC	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32711_Sspa_Oph2007_COI	CNSB-020	1	BE32711_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	GAAGGCCTTCCGATGATC	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32711_Upin_Bot2011_COI	CNSB-020	1	BE32711_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	CTTTAATTACAGCGTTTTTATTGTTGT	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32711_Vgib_Wil2025_COI	CNSB-020	1	BE32711_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	CCCCTCTTTACAAGTTCTTTAGGGT	145.78186
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32712_Asen_Wil2025_COI	CNSB-021	1	BE32712_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	GTTGTTGTTACAACACATGCTCTTTTG	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32712_Cjap_Sim2018_COI	CNSB-021	1	BE32712_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32712_Esin_And2018_Cyb	CNSB-021	1	BE32712_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-021	Cytb	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	ACCCCTCCTCATATCCAACCA	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32712_Mgig_Bot2012_COI	CNSB-021	1	BE32712_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	TCTTATTCGTTGGAGACTTTATAACCCT	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32712_Mjap_Gib2022_COI	CNSB-021	1	BE32712_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	AATTTAATTGTGACTAGGCATGGTCTG	145.78355
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32713_Cmae_Bot2011_COI	CNSB-022	1	BE32713_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	ATGAACAGTCTATCCTCCTTTAG	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32713_Hsan_Wil2021_COI	CNSB-022	1	BE32713_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	CCTGGGCCGGTATAGTAGGT	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32713_Mros_Gib2022_COI	CNSB-022	1	BE32713_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32713_Msal_Bot2012_COI	CNSB-022	1	BE32713_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	GYTAGTTCCRATGATGTTAGCTG	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32713_Mstr_Wil2021_COI	CNSB-022	1	BE32713_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	GTAGTGGTAACTACTCATGCTTTA	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32713_Pvir_Dia2013_COI	CNSB-022	1	BE32713_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	CTTAGTGGCATTAATTCGDAATCC	145.78269
BE32713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92747	WGS84	BE32713	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32713_Rhar_Sim2018_COI	CNSB-022	1	BE32713_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32713	Sample	BE32713	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-022	COI	45	BE32713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-022	CTGCTGCTATTGCTCATG	145.78269
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32715_Aamu_Bax2006_COI	CNSB-024	1	BE32715_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	GCACAACCGGGATCTTTACTTC	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32715_Pcan_Bot2011_IGS	CNSB-024	1	BE32715_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-024	IGS	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	CGTAATCCTCAGTACTGGCTA	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32715_Pper_Dia2013_COI	CNSB-024	1	BE32715_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	CTTAGTGGCATTAATTCGDAATCC	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32715_Sspa_Oph2007_COI	CNSB-024	1	BE32715_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	GAAGGCCTTCCGATGATC	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32715_Upin_Bot2011_COI	CNSB-024	1	BE32715_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	CTTTAATTACAGCGTTTTTATTGTTGT	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32715_Vgib_Wil2025_COI	CNSB-024	1	BE32715_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	CCCCTCTTTACAAGTTCTTTAGGGT	145.78184
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32716_Asen_Wil2025_COI	CNSB-025	1	BE32716_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	GTTGTTGTTACAACACATGCTCTTTTG	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32716_Cjap_Sim2018_COI	CNSB-025	1	BE32716_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32716_Esin_And2018_Cyb	CNSB-025	1	BE32716_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-025	Cytb	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	ACCCCTCCTCATATCCAACCA	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32716_Mjap_Gib2022_COI	CNSB-025	1	BE32716_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	AATTTAATTGTGACTAGGCATGGTCTG	145.78279
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32717_Cmae_Bot2011_COI	CNSB-026	1	BE32717_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	ATGAACAGTCTATCCTCCTTTAG	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32717_Hsan_Wil2021_COI	CNSB-026	1	BE32717_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	CCTGGGCCGGTATAGTAGGT	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32717_Mros_Gib2022_COI	CNSB-026	1	BE32717_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32717_Msal_Bot2012_COI	CNSB-026	1	BE32717_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	GYTAGTTCCRATGATGTTAGCTG	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32717_Mstr_Wil2021_COI	CNSB-026	1	BE32717_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	GTAGTGGTAACTACTCATGCTTTA	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32717_Pvir_Dia2013_COI	CNSB-026	1	BE32717_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	CTTAGTGGCATTAATTCGDAATCC	145.78234
BE32717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92455	WGS84	BE32717	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32717_Rhar_Sim2018_COI	CNSB-026	1	BE32717_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32717	Sample	BE32717	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-026	COI	45	BE32717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-026	CTGCTGCTATTGCTCATG	145.78234
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32719_Aamu_Bax2006_COI	CNSB-028	1	BE32719_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	GCACAACCGGGATCTTTACTTC	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32719_Pcan_Bot2011_IGS	CNSB-028	1	BE32719_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-028	IGS	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	CGTAATCCTCAGTACTGGCTA	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32719_Pper_Dia2013_COI	CNSB-028	1	BE32719_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	CTTAGTGGCATTAATTCGDAATCC	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32719_Sspa_Oph2007_COI	CNSB-028	1	BE32719_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	GAAGGCCTTCCGATGATC	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32719_Upin_Bot2011_COI	CNSB-028	1	BE32719_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	CTTTAATTACAGCGTTTTTATTGTTGT	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32719_Vgib_Wil2025_COI	CNSB-028	1	BE32719_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	CCCCTCTTTACAAGTTCTTTAGGGT	145.78334
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32720_Cmae_Bot2011_COI	CNSB-029	1	BE32720_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	ATGAACAGTCTATCCTCCTTTAG	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32720_Hsan_Wil2021_COI	CNSB-029	1	BE32720_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	CCTGGGCCGGTATAGTAGGT	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32720_Mros_Gib2022_COI	CNSB-029	1	BE32720_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32720_Msal_Bot2012_COI	CNSB-029	1	BE32720_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	GYTAGTTCCRATGATGTTAGCTG	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32720_Mstr_Wil2021_COI	CNSB-029	1	BE32720_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	GTAGTGGTAACTACTCATGCTTTA	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32720_Pvir_Dia2013_COI	CNSB-029	1	BE32720_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	CTTAGTGGCATTAATTCGDAATCC	145.78461
BE32720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92293	WGS84	BE32720	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32720_Rhar_Sim2018_COI	CNSB-029	1	BE32720_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32720	Sample	BE32720	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-029	COI	45	BE32720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-029	CTGCTGCTATTGCTCATG	145.78461
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32722_Aamu_Bax2006_COI	CNSB-031	1	BE32722_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	GCACAACCGGGATCTTTACTTC	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32722_Pcan_Bot2011_IGS	CNSB-031	1	BE32722_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-031	IGS	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	CGTAATCCTCAGTACTGGCTA	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32722_Pper_Dia2013_COI	CNSB-031	1	BE32722_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	CTTAGTGGCATTAATTCGDAATCC	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32722_Sspa_Oph2007_COI	CNSB-031	1	BE32722_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	GAAGGCCTTCCGATGATC	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32722_Upin_Bot2011_COI	CNSB-031	1	BE32722_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	CTTTAATTACAGCGTTTTTATTGTTGT	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32722_Vgib_Wil2025_COI	CNSB-031	1	BE32722_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	CCCCTCTTTACAAGTTCTTTAGGGT	145.78513
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32723_Asen_Wil2025_COI	CNSB-032	1	BE32723_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	GTTGTTGTTACAACACATGCTCTTTTG	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32723_Cjap_Sim2018_COI	CNSB-032	1	BE32723_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32723_Esin_And2018_Cyb	CNSB-032	1	BE32723_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-032	Cytb	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	ACCCCTCCTCATATCCAACCA	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32723_Mgig_Bot2012_COI	CNSB-032	1	BE32723_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	TCTTATTCGTTGGAGACTTTATAACCCT	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32723_Mjap_Gib2022_COI	CNSB-032	1	BE32723_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	AATTTAATTGTGACTAGGCATGGTCTG	145.78453
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32724_Cmae_Bot2011_COI	CNSB-033	1	BE32724_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	ATGAACAGTCTATCCTCCTTTAG	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32724_Hsan_Wil2021_COI	CNSB-033	1	BE32724_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	CCTGGGCCGGTATAGTAGGT	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32724_Mros_Gib2022_COI	CNSB-033	1	BE32724_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32724_Msal_Bot2012_COI	CNSB-033	1	BE32724_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	GYTAGTTCCRATGATGTTAGCTG	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32724_Mstr_Wil2021_COI	CNSB-033	1	BE32724_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	GTAGTGGTAACTACTCATGCTTTA	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32724_Pvir_Dia2013_COI	CNSB-033	1	BE32724_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	CTTAGTGGCATTAATTCGDAATCC	145.78517
BE32724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91855	WGS84	BE32724	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32724_Rhar_Sim2018_COI	CNSB-033	1	BE32724_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32724	Sample	BE32724	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-033	COI	45	BE32724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-033	CTGCTGCTATTGCTCATG	145.78517
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32726_Aamu_Bax2006_COI	CNSB-035	1	BE32726_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	GCACAACCGGGATCTTTACTTC	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32726_Pcan_Bot2011_IGS	CNSB-035	1	BE32726_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-035	IGS	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	CGTAATCCTCAGTACTGGCTA	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32726_Pper_Dia2013_COI	CNSB-035	1	BE32726_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	CTTAGTGGCATTAATTCGDAATCC	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32726_Sspa_Oph2007_COI	CNSB-035	1	BE32726_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	GAAGGCCTTCCGATGATC	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32726_Upin_Bot2011_COI	CNSB-035	1	BE32726_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	CTTTAATTACAGCGTTTTTATTGTTGT	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32726_Vgib_Wil2025_COI	CNSB-035	1	BE32726_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	CCCCTCTTTACAAGTTCTTTAGGGT	145.78546
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32733_Aamu_Bax2006_COI	TSVB-001	1	BE32733_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	GCACAACCGGGATCTTTACTTC	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32733_Pcan_Bot2011_IGS	TSVB-001	1	BE32733_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-001	IGS	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	CGTAATCCTCAGTACTGGCTA	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32733_Pper_Dia2013_COI	TSVB-001	1	BE32733_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	CTTAGTGGCATTAATTCGDAATCC	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32733_Sspa_Oph2007_COI	TSVB-001	1	BE32733_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	GAAGGCCTTCCGATGATC	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32733_Upin_Bot2011_COI	TSVB-001	1	BE32733_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	CTTTAATTACAGCGTTTTTATTGTTGT	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32733_Vgib_Wil2025_COI	TSVB-001	1	BE32733_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	CCCCTCTTTACAAGTTCTTTAGGGT	146.84256
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32734_Asen_Wil2025_COI	TSVB-002	1	BE32734_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	GTTGTTGTTACAACACATGCTCTTTTG	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32734_Cjap_Sim2018_COI	TSVB-002	1	BE32734_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32734_Esin_And2018_Cyb	TSVB-002	1	BE32734_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-002	Cytb	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	ACCCCTCCTCATATCCAACCA	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32734_Mgig_Bot2012_COI	TSVB-002	1	BE32734_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	TCTTATTCGTTGGAGACTTTATAACCCT	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32734_Mjap_Gib2022_COI	TSVB-002	1	BE32734_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	AATTTAATTGTGACTAGGCATGGTCTG	146.84133
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32735_Cmae_Bot2011_COI	TSVB-003	1	BE32735_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	ATGAACAGTCTATCCTCCTTTAG	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32735_Hsan_Wil2021_COI	TSVB-003	1	BE32735_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	CCTGGGCCGGTATAGTAGGT	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32735_Mros_Gib2022_COI	TSVB-003	1	BE32735_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32735_Msal_Bot2012_COI	TSVB-003	1	BE32735_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	GYTAGTTCCRATGATGTTAGCTG	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32735_Mstr_Wil2021_COI	TSVB-003	1	BE32735_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	GTAGTGGTAACTACTCATGCTTTA	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32735_Pvir_Dia2013_COI	TSVB-003	1	BE32735_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	CTTAGTGGCATTAATTCGDAATCC	146.84007
BE32735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24309	WGS84	BE32735	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32735_Rhar_Sim2018_COI	TSVB-003	1	BE32735_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32735	Sample	BE32735	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-003	COI	45	BE32735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-003	CTGCTGCTATTGCTCATG	146.84007
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32737_Aamu_Bax2006_COI	TSVB-005	1	BE32737_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	GCACAACCGGGATCTTTACTTC	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32737_Pcan_Bot2011_IGS	TSVB-005	1	BE32737_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-005	IGS	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	CGTAATCCTCAGTACTGGCTA	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32737_Pper_Dia2013_COI	TSVB-005	1	BE32737_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	CTTAGTGGCATTAATTCGDAATCC	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32737_Sspa_Oph2007_COI	TSVB-005	1	BE32737_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	GAAGGCCTTCCGATGATC	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32737_Upin_Bot2011_COI	TSVB-005	1	BE32737_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	CTTTAATTACAGCGTTTTTATTGTTGT	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32737_Vgib_Wil2025_COI	TSVB-005	1	BE32737_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	CCCCTCTTTACAAGTTCTTTAGGGT	146.84199
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32738_Asen_Wil2025_COI	TSVB-006	1	BE32738_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	GTTGTTGTTACAACACATGCTCTTTTG	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32738_Cjap_Sim2018_COI	TSVB-006	1	BE32738_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32738_Esin_And2018_Cyb	TSVB-006	1	BE32738_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-006	Cytb	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	ACCCCTCCTCATATCCAACCA	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32738_Mgig_Bot2012_COI	TSVB-006	1	BE32738_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	TCTTATTCGTTGGAGACTTTATAACCCT	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32738_Mjap_Gib2022_COI	TSVB-006	1	BE32738_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	AATTTAATTGTGACTAGGCATGGTCTG	146.84104
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32739_Cmae_Bot2011_COI	TSVB-007	1	BE32739_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	ATGAACAGTCTATCCTCCTTTAG	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32739_Mros_Gib2022_COI	TSVB-007	1	BE32739_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32739_Msal_Bot2012_COI	TSVB-007	1	BE32739_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	GYTAGTTCCRATGATGTTAGCTG	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32739_Mstr_Wil2021_COI	TSVB-007	1	BE32739_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	GTAGTGGTAACTACTCATGCTTTA	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32739_Pvir_Dia2013_COI	TSVB-007	1	BE32739_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	CTTAGTGGCATTAATTCGDAATCC	146.8396
BE32739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24014	WGS84	BE32739	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32739_Rhar_Sim2018_COI	TSVB-007	1	BE32739_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32739	Sample	BE32739	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-007	COI	45	BE32739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-007	CTGCTGCTATTGCTCATG	146.8396
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32740_Aamu_Bax2006_COI	TSVB-008	1	BE32740_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	GCACAACCGGGATCTTTACTTC	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32740_Pcan_Bot2011_IGS	TSVB-008	1	BE32740_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-008	IGS	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	CGTAATCCTCAGTACTGGCTA	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32740_Pper_Dia2013_COI	TSVB-008	1	BE32740_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	CTTAGTGGCATTAATTCGDAATCC	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32740_Sspa_Oph2007_COI	TSVB-008	1	BE32740_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	GAAGGCCTTCCGATGATC	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32740_Upin_Bot2011_COI	TSVB-008	1	BE32740_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	CTTTAATTACAGCGTTTTTATTGTTGT	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32740_Vgib_Wil2025_COI	TSVB-008	1	BE32740_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	CCCCTCTTTACAAGTTCTTTAGGGT	146.83793
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32741_Asen_Wil2025_COI	TSVB-009	1	BE32741_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	GTTGTTGTTACAACACATGCTCTTTTG	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32741_Cjap_Sim2018_COI	TSVB-009	1	BE32741_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32741_Esin_And2018_Cyb	TSVB-009	1	BE32741_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-009	Cytb	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	ACCCCTCCTCATATCCAACCA	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32741_Mgig_Bot2012_COI	TSVB-009	1	BE32741_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	TCTTATTCGTTGGAGACTTTATAACCCT	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32741_Mjap_Gib2022_COI	TSVB-009	1	BE32741_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	AATTTAATTGTGACTAGGCATGGTCTG	146.84027
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32742_Cmae_Bot2011_COI	TSVB-010	1	BE32742_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	ATGAACAGTCTATCCTCCTTTAG	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32742_Hsan_Wil2021_COI	TSVB-010	1	BE32742_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	CCTGGGCCGGTATAGTAGGT	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32742_Mros_Gib2022_COI	TSVB-010	1	BE32742_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32742_Msal_Bot2012_COI	TSVB-010	1	BE32742_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	GYTAGTTCCRATGATGTTAGCTG	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32742_Mstr_Wil2021_COI	TSVB-010	1	BE32742_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	GTAGTGGTAACTACTCATGCTTTA	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32742_Pvir_Dia2013_COI	TSVB-010	1	BE32742_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	CTTAGTGGCATTAATTCGDAATCC	146.83856
BE32742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23882	WGS84	BE32742	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32742_Rhar_Sim2018_COI	TSVB-010	1	BE32742_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32742	Sample	BE32742	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-010	COI	45	BE32742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-010	CTGCTGCTATTGCTCATG	146.83856
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32744_Aamu_Bax2006_COI	TSVB-012	1	BE32744_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	GCACAACCGGGATCTTTACTTC	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32744_Pcan_Bot2011_IGS	TSVB-012	1	BE32744_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-012	IGS	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	CGTAATCCTCAGTACTGGCTA	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32744_Pper_Dia2013_COI	TSVB-012	1	BE32744_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	CTTAGTGGCATTAATTCGDAATCC	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32744_Sspa_Oph2007_COI	TSVB-012	1	BE32744_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	GAAGGCCTTCCGATGATC	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32744_Upin_Bot2011_COI	TSVB-012	1	BE32744_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	CTTTAATTACAGCGTTTTTATTGTTGT	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32744_Vgib_Wil2025_COI	TSVB-012	1	BE32744_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	CCCCTCTTTACAAGTTCTTTAGGGT	146.83539
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32745_Asen_Wil2025_COI	TSVB-013	1	BE32745_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	GTTGTTGTTACAACACATGCTCTTTTG	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32745_Cjap_Sim2018_COI	TSVB-013	1	BE32745_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32745_Esin_And2018_Cyb	TSVB-013	1	BE32745_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-013	Cytb	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	ACCCCTCCTCATATCCAACCA	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32745_Mgig_Bot2012_COI	TSVB-013	1	BE32745_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	TCTTATTCGTTGGAGACTTTATAACCCT	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32745_Mjap_Gib2022_COI	TSVB-013	1	BE32745_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	AATTTAATTGTGACTAGGCATGGTCTG	146.83377
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32746_Cmae_Bot2011_COI	TSVB-014	1	BE32746_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	ATGAACAGTCTATCCTCCTTTAG	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32746_Hsan_Wil2021_COI	TSVB-014	1	BE32746_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	CCTGGGCCGGTATAGTAGGT	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32746_Mros_Gib2022_COI	TSVB-014	1	BE32746_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32746_Msal_Bot2012_COI	TSVB-014	1	BE32746_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	GYTAGTTCCRATGATGTTAGCTG	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32746_Mstr_Wil2021_COI	TSVB-014	1	BE32746_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	GTAGTGGTAACTACTCATGCTTTA	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32746_Pvir_Dia2013_COI	TSVB-014	1	BE32746_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	CTTAGTGGCATTAATTCGDAATCC	146.83263
BE32746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23942	WGS84	BE32746	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32746_Rhar_Sim2018_COI	TSVB-014	1	BE32746_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32746	Sample	BE32746	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-014	COI	45	BE32746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-014	CTGCTGCTATTGCTCATG	146.83263
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32748_Aamu_Bax2006_COI	TSVB-016	1	BE32748_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	GCACAACCGGGATCTTTACTTC	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32748_Pcan_Bot2011_IGS	TSVB-016	1	BE32748_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-016	IGS	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	CGTAATCCTCAGTACTGGCTA	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32748_Pper_Dia2013_COI	TSVB-016	1	BE32748_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	CTTAGTGGCATTAATTCGDAATCC	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32748_Sspa_Oph2007_COI	TSVB-016	1	BE32748_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	GAAGGCCTTCCGATGATC	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32748_Upin_Bot2011_COI	TSVB-016	1	BE32748_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	CTTTAATTACAGCGTTTTTATTGTTGT	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32748_Vgib_Wil2025_COI	TSVB-016	1	BE32748_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	CCCCTCTTTACAAGTTCTTTAGGGT	146.83762
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32749_Asen_Wil2025_COI	TSVB-017	1	BE32749_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	GTTGTTGTTACAACACATGCTCTTTTG	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32749_Cjap_Sim2018_COI	TSVB-017	1	BE32749_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32749_Esin_And2018_Cyb	TSVB-017	1	BE32749_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-017	Cytb	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	ACCCCTCCTCATATCCAACCA	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32749_Mgig_Bot2012_COI	TSVB-017	1	BE32749_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	TCTTATTCGTTGGAGACTTTATAACCCT	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32749_Mjap_Gib2022_COI	TSVB-017	1	BE32749_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	AATTTAATTGTGACTAGGCATGGTCTG	146.83614
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32751_Aamu_Bax2006_COI	TSVB-019	1	BE32751_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	GCACAACCGGGATCTTTACTTC	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32751_Pcan_Bot2011_IGS	TSVB-019	1	BE32751_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-019	IGS	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	CGTAATCCTCAGTACTGGCTA	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32751_Pper_Dia2013_COI	TSVB-019	1	BE32751_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	CTTAGTGGCATTAATTCGDAATCC	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32751_Sspa_Oph2007_COI	TSVB-019	1	BE32751_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	GAAGGCCTTCCGATGATC	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32751_Upin_Bot2011_COI	TSVB-019	1	BE32751_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	CTTTAATTACAGCGTTTTTATTGTTGT	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32751_Vgib_Wil2025_COI	TSVB-019	1	BE32751_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	CCCCTCTTTACAAGTTCTTTAGGGT	146.83331
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32752_Asen_Wil2025_COI	TSVB-020	1	BE32752_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	GTTGTTGTTACAACACATGCTCTTTTG	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32752_Cjap_Sim2018_COI	TSVB-020	1	BE32752_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32752_Esin_And2018_Cyb	TSVB-020	1	BE32752_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-020	Cytb	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	ACCCCTCCTCATATCCAACCA	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32752_Mgig_Bot2012_COI	TSVB-020	1	BE32752_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	TCTTATTCGTTGGAGACTTTATAACCCT	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32752_Mjap_Gib2022_COI	TSVB-020	1	BE32752_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	AATTTAATTGTGACTAGGCATGGTCTG	146.83792
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32753_Cmae_Bot2011_COI	TSVB-021	1	BE32753_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	ATGAACAGTCTATCCTCCTTTAG	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32753_Mros_Gib2022_COI	TSVB-021	1	BE32753_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32753_Msal_Bot2012_COI	TSVB-021	1	BE32753_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	GYTAGTTCCRATGATGTTAGCTG	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32753_Mstr_Wil2021_COI	TSVB-021	1	BE32753_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	GTAGTGGTAACTACTCATGCTTTA	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32753_Pvir_Dia2013_COI	TSVB-021	1	BE32753_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	CTTAGTGGCATTAATTCGDAATCC	146.83614
BE32753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23716	WGS84	BE32753	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32753_Rhar_Sim2018_COI	TSVB-021	1	BE32753_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32753	Sample	BE32753	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-021	COI	45	BE32753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-021	CTGCTGCTATTGCTCATG	146.83614
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32755_Aamu_Bax2006_COI	TSVB-023	1	BE32755_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	GCACAACCGGGATCTTTACTTC	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32755_Pcan_Bot2011_IGS	TSVB-023	1	BE32755_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-023	IGS	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	CGTAATCCTCAGTACTGGCTA	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32755_Pper_Dia2013_COI	TSVB-023	1	BE32755_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	CTTAGTGGCATTAATTCGDAATCC	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32755_Sspa_Oph2007_COI	TSVB-023	1	BE32755_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	GAAGGCCTTCCGATGATC	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32755_Upin_Bot2011_COI	TSVB-023	1	BE32755_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	CTTTAATTACAGCGTTTTTATTGTTGT	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32755_Vgib_Wil2025_COI	TSVB-023	1	BE32755_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	CCCCTCTTTACAAGTTCTTTAGGGT	146.83892
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32756_Asen_Wil2025_COI	TSVB-024	1	BE32756_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	GTTGTTGTTACAACACATGCTCTTTTG	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32756_Cjap_Sim2018_COI	TSVB-024	1	BE32756_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32756_Esin_And2018_Cyb	TSVB-024	1	BE32756_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-024	Cytb	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	ACCCCTCCTCATATCCAACCA	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32756_Mgig_Bot2012_COI	TSVB-024	1	BE32756_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	TCTTATTCGTTGGAGACTTTATAACCCT	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32756_Mjap_Gib2022_COI	TSVB-024	1	BE32756_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	AATTTAATTGTGACTAGGCATGGTCTG	146.83443
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32757_Cmae_Bot2011_COI	TSVB-025	1	BE32757_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	ATGAACAGTCTATCCTCCTTTAG	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32757_Hsan_Wil2021_COI	TSVB-025	1	BE32757_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	CCTGGGCCGGTATAGTAGGT	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32757_Mros_Gib2022_COI	TSVB-025	1	BE32757_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32757_Msal_Bot2012_COI	TSVB-025	1	BE32757_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	GYTAGTTCCRATGATGTTAGCTG	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32757_Mstr_Wil2021_COI	TSVB-025	1	BE32757_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	GTAGTGGTAACTACTCATGCTTTA	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32757_Pvir_Dia2013_COI	TSVB-025	1	BE32757_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	CTTAGTGGCATTAATTCGDAATCC	146.83361
BE32757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24514	WGS84	BE32757	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32757_Rhar_Sim2018_COI	TSVB-025	1	BE32757_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32757	Sample	BE32757	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-025	COI	45	BE32757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-025	CTGCTGCTATTGCTCATG	146.83361
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32759_Aamu_Bax2006_COI	TSVB-027	1	BE32759_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	GCACAACCGGGATCTTTACTTC	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32759_Pcan_Bot2011_IGS	TSVB-027	1	BE32759_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-027	IGS	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	CGTAATCCTCAGTACTGGCTA	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32759_Pper_Dia2013_COI	TSVB-027	1	BE32759_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	CTTAGTGGCATTAATTCGDAATCC	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32759_Sspa_Oph2007_COI	TSVB-027	1	BE32759_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	GAAGGCCTTCCGATGATC	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32759_Upin_Bot2011_COI	TSVB-027	1	BE32759_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	CTTTAATTACAGCGTTTTTATTGTTGT	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32759_Vgib_Wil2025_COI	TSVB-027	1	BE32759_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	CCCCTCTTTACAAGTTCTTTAGGGT	146.83533
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32760_Cmae_Bot2011_COI	TSVB-028	1	BE32760_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	ATGAACAGTCTATCCTCCTTTAG	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32760_Hsan_Wil2021_COI	TSVB-028	1	BE32760_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	CCTGGGCCGGTATAGTAGGT	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32760_Mros_Gib2022_COI	TSVB-028	1	BE32760_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32760_Msal_Bot2012_COI	TSVB-028	1	BE32760_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	GYTAGTTCCRATGATGTTAGCTG	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32760_Mstr_Wil2021_COI	TSVB-028	1	BE32760_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	GTAGTGGTAACTACTCATGCTTTA	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32760_Pvir_Dia2013_COI	TSVB-028	1	BE32760_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	CTTAGTGGCATTAATTCGDAATCC	146.83458
BE32760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24726	WGS84	BE32760	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32760_Rhar_Sim2018_COI	TSVB-028	1	BE32760_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32760	Sample	BE32760	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-028	COI	45	BE32760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-028	CTGCTGCTATTGCTCATG	146.83458
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32762_Aamu_Bax2006_COI	TSVB-030	1	BE32762_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	GCACAACCGGGATCTTTACTTC	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32762_Pcan_Bot2011_IGS	TSVB-030	1	BE32762_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-030	IGS	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	CGTAATCCTCAGTACTGGCTA	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32762_Pper_Dia2013_COI	TSVB-030	1	BE32762_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	CTTAGTGGCATTAATTCGDAATCC	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32762_Sspa_Oph2007_COI	TSVB-030	1	BE32762_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	GAAGGCCTTCCGATGATC	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32762_Upin_Bot2011_COI	TSVB-030	1	BE32762_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	CTTTAATTACAGCGTTTTTATTGTTGT	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32762_Vgib_Wil2025_COI	TSVB-030	1	BE32762_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	CCCCTCTTTACAAGTTCTTTAGGGT	146.83326
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32763_Asen_Wil2025_COI	TSVB-031	1	BE32763_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	GTTGTTGTTACAACACATGCTCTTTTG	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32763_Cjap_Sim2018_COI	TSVB-031	1	BE32763_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32763_Esin_And2018_Cyb	TSVB-031	1	BE32763_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-031	Cytb	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	ACCCCTCCTCATATCCAACCA	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32763_Mgig_Bot2012_COI	TSVB-031	1	BE32763_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	TCTTATTCGTTGGAGACTTTATAACCCT	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32763_Mjap_Gib2022_COI	TSVB-031	1	BE32763_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	AATTTAATTGTGACTAGGCATGGTCTG	146.83387
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32764_Cmae_Bot2011_COI	TSVB-032	1	BE32764_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	ATGAACAGTCTATCCTCCTTTAG	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32764_Hsan_Wil2021_COI	TSVB-032	1	BE32764_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	CCTGGGCCGGTATAGTAGGT	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32764_Mros_Gib2022_COI	TSVB-032	1	BE32764_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32764_Msal_Bot2012_COI	TSVB-032	1	BE32764_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	GYTAGTTCCRATGATGTTAGCTG	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32764_Mstr_Wil2021_COI	TSVB-032	1	BE32764_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	GTAGTGGTAACTACTCATGCTTTA	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32764_Pvir_Dia2013_COI	TSVB-032	1	BE32764_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	CTTAGTGGCATTAATTCGDAATCC	146.83404
BE32764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25066	WGS84	BE32764	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32764_Rhar_Sim2018_COI	TSVB-032	1	BE32764_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32764	Sample	BE32764	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-032	COI	45	BE32764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-032	CTGCTGCTATTGCTCATG	146.83404
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32766_Aamu_Bax2006_COI	TSVB-034	1	BE32766_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	GCACAACCGGGATCTTTACTTC	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32766_Pcan_Bot2011_IGS	TSVB-034	1	BE32766_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-034	IGS	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	CGTAATCCTCAGTACTGGCTA	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32766_Pper_Dia2013_COI	TSVB-034	1	BE32766_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	CTTAGTGGCATTAATTCGDAATCC	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32766_Sspa_Oph2007_COI	TSVB-034	1	BE32766_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	GAAGGCCTTCCGATGATC	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32766_Upin_Bot2011_COI	TSVB-034	1	BE32766_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	CTTTAATTACAGCGTTTTTATTGTTGT	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32766_Vgib_Wil2025_COI	TSVB-034	1	BE32766_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	CCCCTCTTTACAAGTTCTTTAGGGT	146.83207
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32767_Asen_Wil2025_COI	TSVB-035	1	BE32767_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	GTTGTTGTTACAACACATGCTCTTTTG	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32767_Cjap_Sim2018_COI	TSVB-035	1	BE32767_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32767_Esin_And2018_Cyb	TSVB-035	1	BE32767_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-035	Cytb	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	ACCCCTCCTCATATCCAACCA	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32767_Mgig_Bot2012_COI	TSVB-035	1	BE32767_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	TCTTATTCGTTGGAGACTTTATAACCCT	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32767_Mjap_Gib2022_COI	TSVB-035	1	BE32767_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	AATTTAATTGTGACTAGGCATGGTCTG	146.83158
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32774_Asen_Wil2025_COI	DRWB-001	1	BE32774_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	GTTGTTGTTACAACACATGCTCTTTTG	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32774_Cjap_Sim2018_COI	DRWB-001	1	BE32774_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32774_Esin_And2018_Cyb	DRWB-001	1	BE32774_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-001	Cytb	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	ACCCCTCCTCATATCCAACCA	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32774_Mgig_Bot2012_COI	DRWB-001	1	BE32774_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	TCTTATTCGTTGGAGACTTTATAACCCT	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32774_Mjap_Gib2022_COI	DRWB-001	1	BE32774_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	AATTTAATTGTGACTAGGCATGGTCTG	130.90592
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32775_Cmae_Bot2011_COI	DRWB-002	1	BE32775_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	ATGAACAGTCTATCCTCCTTTAG	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32775_Hsan_Wil2021_COI	DRWB-002	1	BE32775_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	CCTGGGCCGGTATAGTAGGT	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32775_Mros_Gib2022_COI	DRWB-002	1	BE32775_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32775_Msal_Bot2012_COI	DRWB-002	1	BE32775_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	GYTAGTTCCRATGATGTTAGCTG	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32775_Mstr_Wil2021_COI	DRWB-002	1	BE32775_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	GTAGTGGTAACTACTCATGCTTTA	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32775_Pvir_Dia2013_COI	DRWB-002	1	BE32775_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	CTTAGTGGCATTAATTCGDAATCC	130.90463
BE32775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51321	WGS84	BE32775	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32775_Rhar_Sim2018_COI	DRWB-002	1	BE32775_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32775	Sample	BE32775	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-002	COI	45	BE32775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-002	CTGCTGCTATTGCTCATG	130.90463
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32777_Aamu_Bax2006_COI	DRWB-004	1	BE32777_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	GCACAACCGGGATCTTTACTTC	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32777_Pcan_Bot2011_IGS	DRWB-004	1	BE32777_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-004	IGS	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	CGTAATCCTCAGTACTGGCTA	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32777_Pper_Dia2013_COI	DRWB-004	1	BE32777_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	CTTAGTGGCATTAATTCGDAATCC	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32777_Sspa_Oph2007_COI	DRWB-004	1	BE32777_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	GAAGGCCTTCCGATGATC	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32777_Upin_Bot2011_COI	DRWB-004	1	BE32777_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	CTTTAATTACAGCGTTTTTATTGTTGT	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32777_Vgib_Wil2025_COI	DRWB-004	1	BE32777_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	CCCCTCTTTACAAGTTCTTTAGGGT	130.9043
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32778_Asen_Wil2025_COI	DRWB-005	1	BE32778_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	GTTGTTGTTACAACACATGCTCTTTTG	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32778_Cjap_Sim2018_COI	DRWB-005	1	BE32778_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32778_Esin_And2018_Cyb	DRWB-005	1	BE32778_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-005	Cytb	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	ACCCCTCCTCATATCCAACCA	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32778_Mgig_Bot2012_COI	DRWB-005	1	BE32778_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	TCTTATTCGTTGGAGACTTTATAACCCT	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32778_Mjap_Gib2022_COI	DRWB-005	1	BE32778_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	AATTTAATTGTGACTAGGCATGGTCTG	130.90546
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32779_Cmae_Bot2011_COI	DRWB-006	1	BE32779_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	ATGAACAGTCTATCCTCCTTTAG	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32779_Hsan_Wil2021_COI	DRWB-006	1	BE32779_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	CCTGGGCCGGTATAGTAGGT	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32779_Mros_Gib2022_COI	DRWB-006	1	BE32779_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32779_Msal_Bot2012_COI	DRWB-006	1	BE32779_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	GYTAGTTCCRATGATGTTAGCTG	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32779_Mstr_Wil2021_COI	DRWB-006	1	BE32779_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	GTAGTGGTAACTACTCATGCTTTA	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32779_Pvir_Dia2013_COI	DRWB-006	1	BE32779_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	CTTAGTGGCATTAATTCGDAATCC	130.90367
BE32779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50952	WGS84	BE32779	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32779_Rhar_Sim2018_COI	DRWB-006	1	BE32779_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32779	Sample	BE32779	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-006	COI	45	BE32779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-006	CTGCTGCTATTGCTCATG	130.90367
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32780_Aamu_Bax2006_COI	DRWB-007	1	BE32780_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	GCACAACCGGGATCTTTACTTC	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32780_Pcan_Bot2011_IGS	DRWB-007	1	BE32780_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-007	IGS	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	CGTAATCCTCAGTACTGGCTA	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32780_Pper_Dia2013_COI	DRWB-007	1	BE32780_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	CTTAGTGGCATTAATTCGDAATCC	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32780_Sspa_Oph2007_COI	DRWB-007	1	BE32780_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	GAAGGCCTTCCGATGATC	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32780_Upin_Bot2011_COI	DRWB-007	1	BE32780_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	CTTTAATTACAGCGTTTTTATTGTTGT	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32780_Vgib_Wil2025_COI	DRWB-007	1	BE32780_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	CCCCTCTTTACAAGTTCTTTAGGGT	130.90486
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32781_Asen_Wil2025_COI	DRWB-008	1	BE32781_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	GTTGTTGTTACAACACATGCTCTTTTG	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32781_Cjap_Sim2018_COI	DRWB-008	1	BE32781_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32781_Esin_And2018_Cyb	DRWB-008	1	BE32781_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-008	Cytb	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	ACCCCTCCTCATATCCAACCA	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32781_Mjap_Gib2022_COI	DRWB-008	1	BE32781_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	AATTTAATTGTGACTAGGCATGGTCTG	130.9043
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32782_Cmae_Bot2011_COI	DRWB-009	1	BE32782_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	ATGAACAGTCTATCCTCCTTTAG	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32782_Hsan_Wil2021_COI	DRWB-009	1	BE32782_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	CCTGGGCCGGTATAGTAGGT	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32782_Mros_Gib2022_COI	DRWB-009	1	BE32782_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32782_Msal_Bot2012_COI	DRWB-009	1	BE32782_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	GYTAGTTCCRATGATGTTAGCTG	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32782_Mstr_Wil2021_COI	DRWB-009	1	BE32782_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	GTAGTGGTAACTACTCATGCTTTA	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32782_Pvir_Dia2013_COI	DRWB-009	1	BE32782_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	CTTAGTGGCATTAATTCGDAATCC	130.90555
BE32782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50853	WGS84	BE32782	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32782_Rhar_Sim2018_COI	DRWB-009	1	BE32782_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32782	Sample	BE32782	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-009	COI	45	BE32782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-009	CTGCTGCTATTGCTCATG	130.90555
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32784_Aamu_Bax2006_COI	DRWB-011	1	BE32784_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	GCACAACCGGGATCTTTACTTC	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32784_Pcan_Bot2011_IGS	DRWB-011	1	BE32784_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-011	IGS	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	CGTAATCCTCAGTACTGGCTA	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32784_Pper_Dia2013_COI	DRWB-011	1	BE32784_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	CTTAGTGGCATTAATTCGDAATCC	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32784_Sspa_Oph2007_COI	DRWB-011	1	BE32784_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	GAAGGCCTTCCGATGATC	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32784_Upin_Bot2011_COI	DRWB-011	1	BE32784_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	CTTTAATTACAGCGTTTTTATTGTTGT	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32784_Vgib_Wil2025_COI	DRWB-011	1	BE32784_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	CCCCTCTTTACAAGTTCTTTAGGGT	130.90786
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32785_Asen_Wil2025_COI	DRWB-012	1	BE32785_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	GTTGTTGTTACAACACATGCTCTTTTG	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32785_Cjap_Sim2018_COI	DRWB-012	1	BE32785_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32785_Esin_And2018_Cyb	DRWB-012	1	BE32785_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-012	Cytb	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	ACCCCTCCTCATATCCAACCA	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32785_Mgig_Bot2012_COI	DRWB-012	1	BE32785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	TCTTATTCGTTGGAGACTTTATAACCCT	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32785_Mjap_Gib2022_COI	DRWB-012	1	BE32785_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	AATTTAATTGTGACTAGGCATGGTCTG	130.90373
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32786_Cmae_Bot2011_COI	DRWB-013	1	BE32786_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	ATGAACAGTCTATCCTCCTTTAG	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32786_Hsan_Wil2021_COI	DRWB-013	1	BE32786_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	CCTGGGCCGGTATAGTAGGT	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32786_Mros_Gib2022_COI	DRWB-013	1	BE32786_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32786_Msal_Bot2012_COI	DRWB-013	1	BE32786_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	GYTAGTTCCRATGATGTTAGCTG	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32786_Mstr_Wil2021_COI	DRWB-013	1	BE32786_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	GTAGTGGTAACTACTCATGCTTTA	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32786_Pvir_Dia2013_COI	DRWB-013	1	BE32786_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	CTTAGTGGCATTAATTCGDAATCC	130.90491
BE32786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50757	WGS84	BE32786	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32786_Rhar_Sim2018_COI	DRWB-013	1	BE32786_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32786	Sample	BE32786	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-013	COI	45	BE32786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-013	CTGCTGCTATTGCTCATG	130.90491
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32788_Aamu_Bax2006_COI	DRWB-015	1	BE32788_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	GCACAACCGGGATCTTTACTTC	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32788_Pcan_Bot2011_IGS	DRWB-015	1	BE32788_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-015	IGS	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	CGTAATCCTCAGTACTGGCTA	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32788_Pper_Dia2013_COI	DRWB-015	1	BE32788_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	CTTAGTGGCATTAATTCGDAATCC	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32788_Sspa_Oph2007_COI	DRWB-015	1	BE32788_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	GAAGGCCTTCCGATGATC	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32788_Upin_Bot2011_COI	DRWB-015	1	BE32788_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	CTTTAATTACAGCGTTTTTATTGTTGT	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32788_Vgib_Wil2025_COI	DRWB-015	1	BE32788_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	CCCCTCTTTACAAGTTCTTTAGGGT	130.90725
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32789_Asen_Wil2025_COI	DRWB-016	1	BE32789_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	GTTGTTGTTACAACACATGCTCTTTTG	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32789_Cjap_Sim2018_COI	DRWB-016	1	BE32789_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32789_Esin_And2018_Cyb	DRWB-016	1	BE32789_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-016	Cytb	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	ACCCCTCCTCATATCCAACCA	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32789_Mgig_Bot2012_COI	DRWB-016	1	BE32789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	TCTTATTCGTTGGAGACTTTATAACCCT	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32789_Mjap_Gib2022_COI	DRWB-016	1	BE32789_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	AATTTAATTGTGACTAGGCATGGTCTG	130.90842
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32791_Aamu_Bax2006_COI	DRWB-018	1	BE32791_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	GCACAACCGGGATCTTTACTTC	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32791_Pcan_Bot2011_IGS	DRWB-018	1	BE32791_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-018	IGS	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	CGTAATCCTCAGTACTGGCTA	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32791_Pper_Dia2013_COI	DRWB-018	1	BE32791_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	CTTAGTGGCATTAATTCGDAATCC	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32791_Sspa_Oph2007_COI	DRWB-018	1	BE32791_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	GAAGGCCTTCCGATGATC	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32791_Upin_Bot2011_COI	DRWB-018	1	BE32791_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	CTTTAATTACAGCGTTTTTATTGTTGT	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32791_Vgib_Wil2025_COI	DRWB-018	1	BE32791_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	CCCCTCTTTACAAGTTCTTTAGGGT	130.91082
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32792_Asen_Wil2025_COI	DRWB-019	1	BE32792_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	GTTGTTGTTACAACACATGCTCTTTTG	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32792_Cjap_Sim2018_COI	DRWB-019	1	BE32792_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32792_Esin_And2018_Cyb	DRWB-019	1	BE32792_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-019	Cytb	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	ACCCCTCCTCATATCCAACCA	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32792_Mgig_Bot2012_COI	DRWB-019	1	BE32792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	TCTTATTCGTTGGAGACTTTATAACCCT	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32792_Mjap_Gib2022_COI	DRWB-019	1	BE32792_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	AATTTAATTGTGACTAGGCATGGTCTG	130.91205
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32793_Cmae_Bot2011_COI	DRWB-020	1	BE32793_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	ATGAACAGTCTATCCTCCTTTAG	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32793_Hsan_Wil2021_COI	DRWB-020	1	BE32793_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	CCTGGGCCGGTATAGTAGGT	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32793_Mros_Gib2022_COI	DRWB-020	1	BE32793_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32793_Msal_Bot2012_COI	DRWB-020	1	BE32793_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	GYTAGTTCCRATGATGTTAGCTG	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32793_Mstr_Wil2021_COI	DRWB-020	1	BE32793_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	GTAGTGGTAACTACTCATGCTTTA	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32793_Pvir_Dia2013_COI	DRWB-020	1	BE32793_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	CTTAGTGGCATTAATTCGDAATCC	130.91324
BE32793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32793	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32793_Rhar_Sim2018_COI	DRWB-020	1	BE32793_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32793	Sample	BE32793	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-020	COI	45	BE32793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-020	CTGCTGCTATTGCTCATG	130.91324
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32795_Aamu_Bax2006_COI	DRWB-022	1	BE32795_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	GCACAACCGGGATCTTTACTTC	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32795_Pcan_Bot2011_IGS	DRWB-022	1	BE32795_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-022	IGS	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	CGTAATCCTCAGTACTGGCTA	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32795_Pper_Dia2013_COI	DRWB-022	1	BE32795_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	CTTAGTGGCATTAATTCGDAATCC	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32795_Sspa_Oph2007_COI	DRWB-022	1	BE32795_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	GAAGGCCTTCCGATGATC	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32795_Upin_Bot2011_COI	DRWB-022	1	BE32795_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	CTTTAATTACAGCGTTTTTATTGTTGT	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32795_Vgib_Wil2025_COI	DRWB-022	1	BE32795_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	CCCCTCTTTACAAGTTCTTTAGGGT	130.90676
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32796_Asen_Wil2025_COI	DRWB-023	1	BE32796_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	GTTGTTGTTACAACACATGCTCTTTTG	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32796_Cjap_Sim2018_COI	DRWB-023	1	BE32796_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32796_Esin_And2018_Cyb	DRWB-023	1	BE32796_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-023	Cytb	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	ACCCCTCCTCATATCCAACCA	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32796_Mgig_Bot2012_COI	DRWB-023	1	BE32796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	TCTTATTCGTTGGAGACTTTATAACCCT	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32796_Mjap_Gib2022_COI	DRWB-023	1	BE32796_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	AATTTAATTGTGACTAGGCATGGTCTG	130.90799
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32797_Cmae_Bot2011_COI	DRWB-024	1	BE32797_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	ATGAACAGTCTATCCTCCTTTAG	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32797_Hsan_Wil2021_COI	DRWB-024	1	BE32797_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	CCTGGGCCGGTATAGTAGGT	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32797_Mros_Gib2022_COI	DRWB-024	1	BE32797_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32797_Msal_Bot2012_COI	DRWB-024	1	BE32797_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	GYTAGTTCCRATGATGTTAGCTG	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32797_Mstr_Wil2021_COI	DRWB-024	1	BE32797_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	GTAGTGGTAACTACTCATGCTTTA	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32797_Pvir_Dia2013_COI	DRWB-024	1	BE32797_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	CTTAGTGGCATTAATTCGDAATCC	130.90914
BE32797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32797	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32797_Rhar_Sim2018_COI	DRWB-024	1	BE32797_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32797	Sample	BE32797	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-024	COI	45	BE32797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-024	CTGCTGCTATTGCTCATG	130.90914
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32799_Aamu_Bax2006_COI	DRWB-026	1	BE32799_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	GCACAACCGGGATCTTTACTTC	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32799_Pcan_Bot2011_IGS	DRWB-026	1	BE32799_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-026	IGS	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	CGTAATCCTCAGTACTGGCTA	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32799_Pper_Dia2013_COI	DRWB-026	1	BE32799_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	CTTAGTGGCATTAATTCGDAATCC	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32799_Sspa_Oph2007_COI	DRWB-026	1	BE32799_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	GAAGGCCTTCCGATGATC	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32799_Upin_Bot2011_COI	DRWB-026	1	BE32799_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	CTTTAATTACAGCGTTTTTATTGTTGT	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32799_Vgib_Wil2025_COI	DRWB-026	1	BE32799_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	CCCCTCTTTACAAGTTCTTTAGGGT	130.91155
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32800_Asen_Wil2025_COI	DRWB-027	1	BE32800_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	GTTGTTGTTACAACACATGCTCTTTTG	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32800_Cjap_Sim2018_COI	DRWB-027	1	BE32800_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32800_Esin_And2018_Cyb	DRWB-027	1	BE32800_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-027	Cytb	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	ACCCCTCCTCATATCCAACCA	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32800_Mgig_Bot2012_COI	DRWB-027	1	BE32800_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	TCTTATTCGTTGGAGACTTTATAACCCT	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32800_Mjap_Gib2022_COI	DRWB-027	1	BE32800_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	AATTTAATTGTGACTAGGCATGGTCTG	130.91277
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32801_Cmae_Bot2011_COI	DRWB-028	1	BE32801_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	ATGAACAGTCTATCCTCCTTTAG	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32801_Hsan_Wil2021_COI	DRWB-028	1	BE32801_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	CCTGGGCCGGTATAGTAGGT	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32801_Mros_Gib2022_COI	DRWB-028	1	BE32801_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32801_Msal_Bot2012_COI	DRWB-028	1	BE32801_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	GYTAGTTCCRATGATGTTAGCTG	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32801_Mstr_Wil2021_COI	DRWB-028	1	BE32801_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	GTAGTGGTAACTACTCATGCTTTA	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32801_Pvir_Dia2013_COI	DRWB-028	1	BE32801_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	CTTAGTGGCATTAATTCGDAATCC	130.91384
BE32801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32801	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32801_Rhar_Sim2018_COI	DRWB-028	1	BE32801_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32801	Sample	BE32801	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-028	COI	45	BE32801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-028	CTGCTGCTATTGCTCATG	130.91384
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32803_Aamu_Bax2006_COI	DRWB-030	1	BE32803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	GCACAACCGGGATCTTTACTTC	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32803_Pcan_Bot2011_IGS	DRWB-030	1	BE32803_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-030	IGS	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	CGTAATCCTCAGTACTGGCTA	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32803_Pper_Dia2013_COI	DRWB-030	1	BE32803_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	CTTAGTGGCATTAATTCGDAATCC	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32803_Sspa_Oph2007_COI	DRWB-030	1	BE32803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	GAAGGCCTTCCGATGATC	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32803_Upin_Bot2011_COI	DRWB-030	1	BE32803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	CTTTAATTACAGCGTTTTTATTGTTGT	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32803_Vgib_Wil2025_COI	DRWB-030	1	BE32803_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	CCCCTCTTTACAAGTTCTTTAGGGT	130.90862
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32804_Asen_Wil2025_COI	DRWB-031	1	BE32804_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	GTTGTTGTTACAACACATGCTCTTTTG	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32804_Cjap_Sim2018_COI	DRWB-031	1	BE32804_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32804_Esin_And2018_Cyb	DRWB-031	1	BE32804_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-031	Cytb	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	ACCCCTCCTCATATCCAACCA	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32804_Mgig_Bot2012_COI	DRWB-031	1	BE32804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	TCTTATTCGTTGGAGACTTTATAACCCT	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32804_Mjap_Gib2022_COI	DRWB-031	1	BE32804_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	AATTTAATTGTGACTAGGCATGGTCTG	130.90982
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32805_Cmae_Bot2011_COI	DRWB-032	1	BE32805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	ATGAACAGTCTATCCTCCTTTAG	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32805_Hsan_Wil2021_COI	DRWB-032	1	BE32805_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	CCTGGGCCGGTATAGTAGGT	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32805_Mros_Gib2022_COI	DRWB-032	1	BE32805_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32805_Msal_Bot2012_COI	DRWB-032	1	BE32805_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	GYTAGTTCCRATGATGTTAGCTG	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32805_Mstr_Wil2021_COI	DRWB-032	1	BE32805_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	GTAGTGGTAACTACTCATGCTTTA	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32805_Pvir_Dia2013_COI	DRWB-032	1	BE32805_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	CTTAGTGGCATTAATTCGDAATCC	130.91099
BE32805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50568	WGS84	BE32805	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32805_Rhar_Sim2018_COI	DRWB-032	1	BE32805_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32805	Sample	BE32805	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-032	COI	45	BE32805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-032	CTGCTGCTATTGCTCATG	130.91099
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32807_Aamu_Bax2006_COI	DRWB-034	1	BE32807_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	GCACAACCGGGATCTTTACTTC	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32807_Pcan_Bot2011_IGS	DRWB-034	1	BE32807_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-034	IGS	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	CGTAATCCTCAGTACTGGCTA	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32807_Pper_Dia2013_COI	DRWB-034	1	BE32807_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	CTTAGTGGCATTAATTCGDAATCC	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32807_Sspa_Oph2007_COI	DRWB-034	1	BE32807_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	GAAGGCCTTCCGATGATC	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32807_Upin_Bot2011_COI	DRWB-034	1	BE32807_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	CTTTAATTACAGCGTTTTTATTGTTGT	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32807_Vgib_Wil2025_COI	DRWB-034	1	BE32807_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	CCCCTCTTTACAAGTTCTTTAGGGT	130.91346
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32808_Asen_Wil2025_COI	DRWB-035	1	BE32808_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	GTTGTTGTTACAACACATGCTCTTTTG	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32808_Cjap_Sim2018_COI	DRWB-035	1	BE32808_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32808_Esin_And2018_Cyb	DRWB-035	1	BE32808_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-035	Cytb	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	ACCCCTCCTCATATCCAACCA	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32808_Mgig_Bot2012_COI	DRWB-035	1	BE32808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	TCTTATTCGTTGGAGACTTTATAACCCT	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32808_Mjap_Gib2022_COI	DRWB-035	1	BE32808_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	AATTTAATTGTGACTAGGCATGGTCTG	130.91296
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32815_Asen_Wil2025_COI	DRWB-036	1	BE32815_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	GTTGTTGTTACAACACATGCTCTTTTG	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32815_Cjap_Sim2018_COI	DRWB-036	1	BE32815_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32815_Esin_And2018_Cyb	DRWB-036	1	BE32815_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-036	Cytb	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	ACCCCTCCTCATATCCAACCA	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32815_Mgig_Bot2012_COI	DRWB-036	1	BE32815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	TCTTATTCGTTGGAGACTTTATAACCCT	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32815_Mjap_Gib2022_COI	DRWB-036	1	BE32815_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	AATTTAATTGTGACTAGGCATGGTCTG	130.84724
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32816_Cmae_Bot2011_COI	DRWB-037	1	BE32816_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	ATGAACAGTCTATCCTCCTTTAG	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32816_Hsan_Wil2021_COI	DRWB-037	1	BE32816_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	CCTGGGCCGGTATAGTAGGT	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32816_Mros_Gib2022_COI	DRWB-037	1	BE32816_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32816_Msal_Bot2012_COI	DRWB-037	1	BE32816_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	GYTAGTTCCRATGATGTTAGCTG	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32816_Mstr_Wil2021_COI	DRWB-037	1	BE32816_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	GTAGTGGTAACTACTCATGCTTTA	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32816_Pvir_Dia2013_COI	DRWB-037	1	BE32816_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	CTTAGTGGCATTAATTCGDAATCC	130.84861
BE32816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52772	WGS84	BE32816	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32816_Rhar_Sim2018_COI	DRWB-037	1	BE32816_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32816	Sample	BE32816	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-037	COI	45	BE32816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-037	CTGCTGCTATTGCTCATG	130.84861
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32818_Aamu_Bax2006_COI	DRWB-039	1	BE32818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	GCACAACCGGGATCTTTACTTC	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32818_Pcan_Bot2011_IGS	DRWB-039	1	BE32818_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-039	IGS	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	CGTAATCCTCAGTACTGGCTA	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32818_Pper_Dia2013_COI	DRWB-039	1	BE32818_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	CTTAGTGGCATTAATTCGDAATCC	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32818_Sspa_Oph2007_COI	DRWB-039	1	BE32818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	GAAGGCCTTCCGATGATC	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32818_Upin_Bot2011_COI	DRWB-039	1	BE32818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	CTTTAATTACAGCGTTTTTATTGTTGT	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32818_Vgib_Wil2025_COI	DRWB-039	1	BE32818_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	CCCCTCTTTACAAGTTCTTTAGGGT	130.85119
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32819_Asen_Wil2025_COI	DRWB-040	1	BE32819_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	GTTGTTGTTACAACACATGCTCTTTTG	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32819_Cjap_Sim2018_COI	DRWB-040	1	BE32819_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32819_Esin_And2018_Cyb	DRWB-040	1	BE32819_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-040	Cytb	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	ACCCCTCCTCATATCCAACCA	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32819_Mjap_Gib2022_COI	DRWB-040	1	BE32819_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	AATTTAATTGTGACTAGGCATGGTCTG	130.85255
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32821_Aamu_Bax2006_COI	DRWB-042	1	BE32821_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	GCACAACCGGGATCTTTACTTC	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32821_Pcan_Bot2011_IGS	DRWB-042	1	BE32821_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-042	IGS	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	CGTAATCCTCAGTACTGGCTA	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32821_Pper_Dia2013_COI	DRWB-042	1	BE32821_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	CTTAGTGGCATTAATTCGDAATCC	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32821_Sspa_Oph2007_COI	DRWB-042	1	BE32821_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	GAAGGCCTTCCGATGATC	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32821_Upin_Bot2011_COI	DRWB-042	1	BE32821_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	CTTTAATTACAGCGTTTTTATTGTTGT	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32821_Vgib_Wil2025_COI	DRWB-042	1	BE32821_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	CCCCTCTTTACAAGTTCTTTAGGGT	130.84791
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32822_Asen_Wil2025_COI	DRWB-043	1	BE32822_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	GTTGTTGTTACAACACATGCTCTTTTG	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32822_Cjap_Sim2018_COI	DRWB-043	1	BE32822_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32822_Esin_And2018_Cyb	DRWB-043	1	BE32822_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-043	Cytb	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	ACCCCTCCTCATATCCAACCA	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32822_Mgig_Bot2012_COI	DRWB-043	1	BE32822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	TCTTATTCGTTGGAGACTTTATAACCCT	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32822_Mjap_Gib2022_COI	DRWB-043	1	BE32822_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	AATTTAATTGTGACTAGGCATGGTCTG	130.84921
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32823_Cmae_Bot2011_COI	DRWB-044	1	BE32823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	ATGAACAGTCTATCCTCCTTTAG	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32823_Hsan_Wil2021_COI	DRWB-044	1	BE32823_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	CCTGGGCCGGTATAGTAGGT	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32823_Mros_Gib2022_COI	DRWB-044	1	BE32823_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32823_Msal_Bot2012_COI	DRWB-044	1	BE32823_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	GYTAGTTCCRATGATGTTAGCTG	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32823_Mstr_Wil2021_COI	DRWB-044	1	BE32823_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	GTAGTGGTAACTACTCATGCTTTA	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32823_Pvir_Dia2013_COI	DRWB-044	1	BE32823_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	CTTAGTGGCATTAATTCGDAATCC	130.85045
BE32823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52671	WGS84	BE32823	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32823_Rhar_Sim2018_COI	DRWB-044	1	BE32823_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32823	Sample	BE32823	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-044	COI	45	BE32823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-044	CTGCTGCTATTGCTCATG	130.85045
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32825_Aamu_Bax2006_COI	DRWB-046	1	BE32825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	GCACAACCGGGATCTTTACTTC	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32825_Pcan_Bot2011_IGS	DRWB-046	1	BE32825_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-046	IGS	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	CGTAATCCTCAGTACTGGCTA	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32825_Pper_Dia2013_COI	DRWB-046	1	BE32825_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	CTTAGTGGCATTAATTCGDAATCC	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32825_Sspa_Oph2007_COI	DRWB-046	1	BE32825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	GAAGGCCTTCCGATGATC	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32825_Upin_Bot2011_COI	DRWB-046	1	BE32825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	CTTTAATTACAGCGTTTTTATTGTTGT	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32825_Vgib_Wil2025_COI	DRWB-046	1	BE32825_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	CCCCTCTTTACAAGTTCTTTAGGGT	130.85325
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32826_Asen_Wil2025_COI	DRWB-047	1	BE32826_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	GTTGTTGTTACAACACATGCTCTTTTG	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32826_Cjap_Sim2018_COI	DRWB-047	1	BE32826_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32826_Esin_And2018_Cyb	DRWB-047	1	BE32826_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-047	Cytb	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	ACCCCTCCTCATATCCAACCA	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32826_Mjap_Gib2022_COI	DRWB-047	1	BE32826_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	AATTTAATTGTGACTAGGCATGGTCTG	130.84593
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32827_Cmae_Bot2011_COI	DRWB-048	1	BE32827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	ATGAACAGTCTATCCTCCTTTAG	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32827_Hsan_Wil2021_COI	DRWB-048	1	BE32827_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	CCTGGGCCGGTATAGTAGGT	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32827_Mros_Gib2022_COI	DRWB-048	1	BE32827_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32827_Msal_Bot2012_COI	DRWB-048	1	BE32827_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	GYTAGTTCCRATGATGTTAGCTG	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32827_Mstr_Wil2021_COI	DRWB-048	1	BE32827_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	GTAGTGGTAACTACTCATGCTTTA	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32827_Pvir_Dia2013_COI	DRWB-048	1	BE32827_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	CTTAGTGGCATTAATTCGDAATCC	130.8473
BE32827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52539	WGS84	BE32827	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32827_Rhar_Sim2018_COI	DRWB-048	1	BE32827_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32827	Sample	BE32827	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-048	COI	45	BE32827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-048	CTGCTGCTATTGCTCATG	130.8473
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32829_Aamu_Bax2006_COI	DRWB-050	1	BE32829_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	GCACAACCGGGATCTTTACTTC	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32829_Pcan_Bot2011_IGS	DRWB-050	1	BE32829_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-050	IGS	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	CGTAATCCTCAGTACTGGCTA	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32829_Pper_Dia2013_COI	DRWB-050	1	BE32829_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	CTTAGTGGCATTAATTCGDAATCC	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32829_Sspa_Oph2007_COI	DRWB-050	1	BE32829_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	GAAGGCCTTCCGATGATC	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32829_Upin_Bot2011_COI	DRWB-050	1	BE32829_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	CTTTAATTACAGCGTTTTTATTGTTGT	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32829_Vgib_Wil2025_COI	DRWB-050	1	BE32829_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	CCCCTCTTTACAAGTTCTTTAGGGT	130.84662
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32830_Cmae_Bot2011_COI	DRWB-051	1	BE32830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	ATGAACAGTCTATCCTCCTTTAG	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32830_Hsan_Wil2021_COI	DRWB-051	1	BE32830_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	CCTGGGCCGGTATAGTAGGT	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32830_Mros_Gib2022_COI	DRWB-051	1	BE32830_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32830_Msal_Bot2012_COI	DRWB-051	1	BE32830_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	GYTAGTTCCRATGATGTTAGCTG	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32830_Mstr_Wil2021_COI	DRWB-051	1	BE32830_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	GTAGTGGTAACTACTCATGCTTTA	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32830_Pvir_Dia2013_COI	DRWB-051	1	BE32830_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	CTTAGTGGCATTAATTCGDAATCC	130.848
BE32830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5243	WGS84	BE32830	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32830_Rhar_Sim2018_COI	DRWB-051	1	BE32830_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32830	Sample	BE32830	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-051	COI	45	BE32830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-051	CTGCTGCTATTGCTCATG	130.848
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32832_Aamu_Bax2006_COI	DRWB-053	1	BE32832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	GCACAACCGGGATCTTTACTTC	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32832_Pcan_Bot2011_IGS	DRWB-053	1	BE32832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-053	IGS	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	CGTAATCCTCAGTACTGGCTA	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32832_Pper_Dia2013_COI	DRWB-053	1	BE32832_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	CTTAGTGGCATTAATTCGDAATCC	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32832_Sspa_Oph2007_COI	DRWB-053	1	BE32832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	GAAGGCCTTCCGATGATC	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32832_Upin_Bot2011_COI	DRWB-053	1	BE32832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	CTTTAATTACAGCGTTTTTATTGTTGT	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32832_Vgib_Wil2025_COI	DRWB-053	1	BE32832_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	CCCCTCTTTACAAGTTCTTTAGGGT	130.84465
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32833_Asen_Wil2025_COI	DRWB-054	1	BE32833_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	GTTGTTGTTACAACACATGCTCTTTTG	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32833_Cjap_Sim2018_COI	DRWB-054	1	BE32833_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32833_Esin_And2018_Cyb	DRWB-054	1	BE32833_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-054	Cytb	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	ACCCCTCCTCATATCCAACCA	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32833_Mgig_Bot2012_COI	DRWB-054	1	BE32833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	TCTTATTCGTTGGAGACTTTATAACCCT	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32833_Mjap_Gib2022_COI	DRWB-054	1	BE32833_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	AATTTAATTGTGACTAGGCATGGTCTG	130.84601
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32834_Cmae_Bot2011_COI	DRWB-055	1	BE32834_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	ATGAACAGTCTATCCTCCTTTAG	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32834_Hsan_Wil2021_COI	DRWB-055	1	BE32834_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	CCTGGGCCGGTATAGTAGGT	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32834_Mros_Gib2022_COI	DRWB-055	1	BE32834_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32834_Msal_Bot2012_COI	DRWB-055	1	BE32834_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	GYTAGTTCCRATGATGTTAGCTG	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32834_Mstr_Wil2021_COI	DRWB-055	1	BE32834_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	GTAGTGGTAACTACTCATGCTTTA	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32834_Pvir_Dia2013_COI	DRWB-055	1	BE32834_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	CTTAGTGGCATTAATTCGDAATCC	130.84732
BE32834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52329	WGS84	BE32834	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32834_Rhar_Sim2018_COI	DRWB-055	1	BE32834_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32834	Sample	BE32834	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-055	COI	45	BE32834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-055	CTGCTGCTATTGCTCATG	130.84732
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32836_Aamu_Bax2006_COI	DRWB-057	1	BE32836_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	GCACAACCGGGATCTTTACTTC	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32836_Pcan_Bot2011_IGS	DRWB-057	1	BE32836_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-057	IGS	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	CGTAATCCTCAGTACTGGCTA	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32836_Pper_Dia2013_COI	DRWB-057	1	BE32836_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	CTTAGTGGCATTAATTCGDAATCC	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32836_Sspa_Oph2007_COI	DRWB-057	1	BE32836_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	GAAGGCCTTCCGATGATC	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32836_Upin_Bot2011_COI	DRWB-057	1	BE32836_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	CTTTAATTACAGCGTTTTTATTGTTGT	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32836_Vgib_Wil2025_COI	DRWB-057	1	BE32836_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	CCCCTCTTTACAAGTTCTTTAGGGT	130.84667
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32837_Asen_Wil2025_COI	DRWB-058	1	BE32837_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	GTTGTTGTTACAACACATGCTCTTTTG	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32837_Cjap_Sim2018_COI	DRWB-058	1	BE32837_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32837_Esin_And2018_Cyb	DRWB-058	1	BE32837_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-058	Cytb	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	ACCCCTCCTCATATCCAACCA	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32837_Mgig_Bot2012_COI	DRWB-058	1	BE32837_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	TCTTATTCGTTGGAGACTTTATAACCCT	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32837_Mjap_Gib2022_COI	DRWB-058	1	BE32837_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	AATTTAATTGTGACTAGGCATGGTCTG	130.85111
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32838_Cmae_Bot2011_COI	DRWB-059	1	BE32838_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	ATGAACAGTCTATCCTCCTTTAG	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32838_Hsan_Wil2021_COI	DRWB-059	1	BE32838_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	CCTGGGCCGGTATAGTAGGT	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32838_Mros_Gib2022_COI	DRWB-059	1	BE32838_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32838_Msal_Bot2012_COI	DRWB-059	1	BE32838_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	GYTAGTTCCRATGATGTTAGCTG	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32838_Mstr_Wil2021_COI	DRWB-059	1	BE32838_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	GTAGTGGTAACTACTCATGCTTTA	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32838_Pvir_Dia2013_COI	DRWB-059	1	BE32838_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	CTTAGTGGCATTAATTCGDAATCC	130.84464
BE32838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52102	WGS84	BE32838	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32838_Rhar_Sim2018_COI	DRWB-059	1	BE32838_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32838	Sample	BE32838	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-059	COI	45	BE32838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-059	CTGCTGCTATTGCTCATG	130.84464
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32840_Asen_Wil2025_COI	DRWB-061	1	BE32840_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	GTTGTTGTTACAACACATGCTCTTTTG	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32840_Cjap_Sim2018_COI	DRWB-061	1	BE32840_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32840_Esin_And2018_Cyb	DRWB-061	1	BE32840_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-061	Cytb	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	ACCCCTCCTCATATCCAACCA	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32840_Mgig_Bot2012_COI	DRWB-061	1	BE32840_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	TCTTATTCGTTGGAGACTTTATAACCCT	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32840_Mjap_Gib2022_COI	DRWB-061	1	BE32840_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	AATTTAATTGTGACTAGGCATGGTCTG	130.84739
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32841_Cmae_Bot2011_COI	DRWB-062	1	BE32841_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	ATGAACAGTCTATCCTCCTTTAG	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32841_Hsan_Wil2021_COI	DRWB-062	1	BE32841_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	CCTGGGCCGGTATAGTAGGT	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32841_Mros_Gib2022_COI	DRWB-062	1	BE32841_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32841_Msal_Bot2012_COI	DRWB-062	1	BE32841_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	GYTAGTTCCRATGATGTTAGCTG	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32841_Mstr_Wil2021_COI	DRWB-062	1	BE32841_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	GTAGTGGTAACTACTCATGCTTTA	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32841_Pvir_Dia2013_COI	DRWB-062	1	BE32841_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	CTTAGTGGCATTAATTCGDAATCC	130.84871
BE32841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.521	WGS84	BE32841	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32841_Rhar_Sim2018_COI	DRWB-062	1	BE32841_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32841	Sample	BE32841	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-062	COI	45	BE32841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-062	CTGCTGCTATTGCTCATG	130.84871
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32843_Aamu_Bax2006_COI	DRWB-064	1	BE32843_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	GCACAACCGGGATCTTTACTTC	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32843_Pcan_Bot2011_IGS	DRWB-064	1	BE32843_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-064	IGS	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	CGTAATCCTCAGTACTGGCTA	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32843_Pper_Dia2013_COI	DRWB-064	1	BE32843_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	CTTAGTGGCATTAATTCGDAATCC	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32843_Sspa_Oph2007_COI	DRWB-064	1	BE32843_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	GAAGGCCTTCCGATGATC	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32843_Upin_Bot2011_COI	DRWB-064	1	BE32843_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	CTTTAATTACAGCGTTTTTATTGTTGT	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32843_Vgib_Wil2025_COI	DRWB-064	1	BE32843_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	CCCCTCTTTACAAGTTCTTTAGGGT	130.84544
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32844_Asen_Wil2025_COI	DRWB-065	1	BE32844_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	GTTGTTGTTACAACACATGCTCTTTTG	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32844_Cjap_Sim2018_COI	DRWB-065	1	BE32844_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32844_Esin_And2018_Cyb	DRWB-065	1	BE32844_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-065	Cytb	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	ACCCCTCCTCATATCCAACCA	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32844_Mgig_Bot2012_COI	DRWB-065	1	BE32844_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	TCTTATTCGTTGGAGACTTTATAACCCT	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32844_Mjap_Gib2022_COI	DRWB-065	1	BE32844_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	AATTTAATTGTGACTAGGCATGGTCTG	130.84676
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32845_Cmae_Bot2011_COI	DRWB-066	1	BE32845_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	ATGAACAGTCTATCCTCCTTTAG	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32845_Hsan_Wil2021_COI	DRWB-066	1	BE32845_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	CCTGGGCCGGTATAGTAGGT	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32845_Mros_Gib2022_COI	DRWB-066	1	BE32845_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32845_Msal_Bot2012_COI	DRWB-066	1	BE32845_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	GYTAGTTCCRATGATGTTAGCTG	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32845_Mstr_Wil2021_COI	DRWB-066	1	BE32845_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	GTAGTGGTAACTACTCATGCTTTA	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32845_Pvir_Dia2013_COI	DRWB-066	1	BE32845_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	CTTAGTGGCATTAATTCGDAATCC	130.8481
BE32845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51987	WGS84	BE32845	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32845_Rhar_Sim2018_COI	DRWB-066	1	BE32845_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32845	Sample	BE32845	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-066	COI	45	BE32845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-066	CTGCTGCTATTGCTCATG	130.8481
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32847_Aamu_Bax2006_COI	DRWB-068	1	BE32847_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	GCACAACCGGGATCTTTACTTC	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32847_Pcan_Bot2011_IGS	DRWB-068	1	BE32847_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-068	IGS	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	CGTAATCCTCAGTACTGGCTA	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32847_Pper_Dia2013_COI	DRWB-068	1	BE32847_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	CTTAGTGGCATTAATTCGDAATCC	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32847_Sspa_Oph2007_COI	DRWB-068	1	BE32847_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	GAAGGCCTTCCGATGATC	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32847_Upin_Bot2011_COI	DRWB-068	1	BE32847_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	CTTTAATTACAGCGTTTTTATTGTTGT	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32847_Vgib_Wil2025_COI	DRWB-068	1	BE32847_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	CCCCTCTTTACAAGTTCTTTAGGGT	130.84744
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32848_Asen_Wil2025_COI	DRWB-069	1	BE32848_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	GTTGTTGTTACAACACATGCTCTTTTG	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32848_Cjap_Sim2018_COI	DRWB-069	1	BE32848_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32848_Esin_And2018_Cyb	DRWB-069	1	BE32848_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-069	Cytb	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	ACCCCTCCTCATATCCAACCA	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32848_Mgig_Bot2012_COI	DRWB-069	1	BE32848_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	TCTTATTCGTTGGAGACTTTATAACCCT	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32848_Mjap_Gib2022_COI	DRWB-069	1	BE32848_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	AATTTAATTGTGACTAGGCATGGTCTG	130.84876
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32849_Cmae_Bot2011_COI	DRWB-070	1	BE32849_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	ATGAACAGTCTATCCTCCTTTAG	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32849_Hsan_Wil2021_COI	DRWB-070	1	BE32849_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	CCTGGGCCGGTATAGTAGGT	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32849_Mros_Gib2022_COI	DRWB-070	1	BE32849_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32849_Msal_Bot2012_COI	DRWB-070	1	BE32849_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	GYTAGTTCCRATGATGTTAGCTG	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32849_Mstr_Wil2021_COI	DRWB-070	1	BE32849_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	GTAGTGGTAACTACTCATGCTTTA	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32849_Pvir_Dia2013_COI	DRWB-070	1	BE32849_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	CTTAGTGGCATTAATTCGDAATCC	130.85013
BE32849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32849	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32849_Rhar_Sim2018_COI	DRWB-070	1	BE32849_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32849	Sample	BE32849	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-070	COI	45	BE32849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-070	CTGCTGCTATTGCTCATG	130.85013
id	detectionCriteria	pcrPlateID	decimalLatitude	geodeticDatum	materialSampleID	pcrProbeSeq	pcrProbeRepoter	ampVisMethod	basisOfRecord	quantificationCycle	coordinateUncertaintyInMetres	targetTaxonomicAssay	sampleMaterialProcess	pcrInhibitionScaleFactor	pcrProbeQuencher	associatedReferences	parentEventID	eventDate	pcrPrimerREV	biome	scientificName	pcrEfficiency	pcrAmpliconSize	catalogueNumber	sourceMaterialID	pcrInhibitionCheck01	occurrenceID	samplingProtocol	sampleCollectionMethod	eventID	eventTypeHierarchy	sampleName	assayType	occurrenceStatus	eventType	assayName	materialEntityID	pcrTargetGene	pcrCycles	eventHierarchy	coordinatePrecision	pcrInhibitionCheck	country	fieldNumber	pcrPrimerFWD	decimalLongitude
BA77713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05372	-34.83595	WGS84	BA77713	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	28.54	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_2	3/05/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA77713_Cmae_Bot2011_COI	C-NS-D2	1	BA77713_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA77713	Sample	BA77713	targeted	Present	Sample	Cmae_Bot2011_COI	C-NS-D2	COI	45	BA77713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C-NS-D2	ATGAACAGTCTATCCTCCTTTAG	138.50736
BA83921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05434	-34.80981	WGS84	BA83921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.045	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83921_Cmae_Bot2011_COI	A23	1	BA83921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83921	Sample	BA83921	targeted	Present	Sample	Cmae_Bot2011_COI	A23	COI	45	BA83921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A23	ATGAACAGTCTATCCTCCTTTAG	138.51833
BA83925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05434	-34.81103	WGS84	BA83925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.426	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83925_Cmae_Bot2011_COI	A39	1	BA83925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83925	Sample	BA83925	targeted	Present	Sample	Cmae_Bot2011_COI	A39	COI	45	BA83925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A39	ATGAACAGTCTATCCTCCTTTAG	138.52253
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83963_Aamu_Bax2006_COI	C5	1	BA83963_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Aamu_Bax2006_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	GCACAACCGGGATCTTTACTTC	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83963_Pcan_Bot2011_IGS	C5	1	BA83963_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Pcan_Bot2011_IGS	C5	IGS	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	CGTAATCCTCAGTACTGGCTA	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83963_Pper_Dia2013_COI	C5	1	BA83963_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Pper_Dia2013_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	CTTAGTGGCATTAATTCGDAATCC	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83963_Sspa_Oph2007_COI	C5	1	BA83963_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Sspa_Oph2007_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	GAAGGCCTTCCGATGATC	145.79636
BA83963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.95949	WGS84	BA83963	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83963_Upin_Bot2011_COI	C5	1	BA83963_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83963	Sample	BA83963	targeted	Absent	Sample	Upin_Bot2011_COI	C5	COI	45	BA83963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C5	CTTTAATTACAGCGTTTTTATTGTTGT	145.79636
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83964_Cjap_Sim2018_COI	C6	1	BA83964_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Cjap_Sim2018_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83964_Esin_And2018_Cyb	C6	1	BA83964_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Esin_And2018_Cyb	C6	Cytb	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	ACCCCTCCTCATATCCAACCA	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83964_Mgig_Bot2012_COI	C6	1	BA83964_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Mgig_Bot2012_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	TCTTATTCGTTGGAGACTTTATAACCCT	145.7847
BA83964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92137	WGS84	BA83964	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA83964_Mjap_Gib2022_COI	C6	1	BA83964_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83964	Sample	BA83964	targeted	Absent	Sample	Mjap_Gib2022_COI	C6	COI	45	BA83964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C6	AATTTAATTGTGACTAGGCATGGTCTG	145.7847
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83965_Cmae_Bot2011_COI	C10	1	BA83965_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Cmae_Bot2011_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	ATGAACAGTCTATCCTCCTTTAG	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83965_Hsan_Wil2021_COI	C10	1	BA83965_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Hsan_Wil2021_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	CCTGGGCCGGTATAGTAGGT	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83965_Mros_Gib2022_COI	C10	1	BA83965_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Mros_Gib2022_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83965_Msal_Bot2012_COI	C10	1	BA83965_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Msal_Bot2012_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	GYTAGTTCCRATGATGTTAGCTG	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83965_Pvir_Dia2013_COI	C10	1	BA83965_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Pvir_Dia2013_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	CTTAGTGGCATTAATTCGDAATCC	145.78334
BA83965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83965	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83965_Rhar_Sim2018_COI	C10	1	BA83965_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83965	Sample	BA83965	targeted	Absent	Sample	Rhar_Sim2018_COI	C10	COI	45	BA83965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C10	CTGCTGCTATTGCTCATG	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83967_Aamu_Bax2006_COI	C12	1	BA83967_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Aamu_Bax2006_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	GCACAACCGGGATCTTTACTTC	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83967_Pcan_Bot2011_IGS	C12	1	BA83967_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Pcan_Bot2011_IGS	C12	IGS	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	CGTAATCCTCAGTACTGGCTA	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83967_Pper_Dia2013_COI	C12	1	BA83967_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Pper_Dia2013_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	CTTAGTGGCATTAATTCGDAATCC	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83967_Sspa_Oph2007_COI	C12	1	BA83967_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Sspa_Oph2007_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	GAAGGCCTTCCGATGATC	145.78334
BA83967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92728	WGS84	BA83967	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83967_Upin_Bot2011_COI	C12	1	BA83967_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83967	Sample	BA83967	targeted	Absent	Sample	Upin_Bot2011_COI	C12	COI	45	BA83967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C12	CTTTAATTACAGCGTTTTTATTGTTGT	145.78334
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83968_Cjap_Sim2018_COI	C16	1	BA83968_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Cjap_Sim2018_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83968_Esin_And2018_Cyb	C16	1	BA83968_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Esin_And2018_Cyb	C16	Cytb	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	ACCCCTCCTCATATCCAACCA	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83968_Mgig_Bot2012_COI	C16	1	BA83968_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Mgig_Bot2012_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	TCTTATTCGTTGGAGACTTTATAACCCT	145.78604
BA83968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83968	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA83968_Mjap_Gib2022_COI	C16	1	BA83968_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83968	Sample	BA83968	targeted	Absent	Sample	Mjap_Gib2022_COI	C16	COI	45	BA83968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C16	AATTTAATTGTGACTAGGCATGGTCTG	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83969_Cmae_Bot2011_COI	C17	1	BA83969_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Cmae_Bot2011_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	ATGAACAGTCTATCCTCCTTTAG	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83969_Hsan_Wil2021_COI	C17	1	BA83969_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Hsan_Wil2021_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	CCTGGGCCGGTATAGTAGGT	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83969_Mros_Gib2022_COI	C17	1	BA83969_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Mros_Gib2022_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83969_Msal_Bot2012_COI	C17	1	BA83969_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Msal_Bot2012_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	GYTAGTTCCRATGATGTTAGCTG	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83969_Pvir_Dia2013_COI	C17	1	BA83969_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Pvir_Dia2013_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	CTTAGTGGCATTAATTCGDAATCC	145.78604
BA83969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94477	WGS84	BA83969	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83969_Rhar_Sim2018_COI	C17	1	BA83969_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83969	Sample	BA83969	targeted	Absent	Sample	Rhar_Sim2018_COI	C17	COI	45	BA83969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C17	CTGCTGCTATTGCTCATG	145.78604
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83970_Aamu_Bax2006_COI	C18	1	BA83970_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Aamu_Bax2006_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	GCACAACCGGGATCTTTACTTC	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83970_Pcan_Bot2011_IGS	C18	1	BA83970_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Pcan_Bot2011_IGS	C18	IGS	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	CGTAATCCTCAGTACTGGCTA	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83970_Pper_Dia2013_COI	C18	1	BA83970_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Pper_Dia2013_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	CTTAGTGGCATTAATTCGDAATCC	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83970_Sspa_Oph2007_COI	C18	1	BA83970_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Sspa_Oph2007_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	GAAGGCCTTCCGATGATC	145.78312
BA83970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83970	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83970_Upin_Bot2011_COI	C18	1	BA83970_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83970	Sample	BA83970	targeted	Absent	Sample	Upin_Bot2011_COI	C18	COI	45	BA83970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C18	CTTTAATTACAGCGTTTTTATTGTTGT	145.78312
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83971_Cjap_Sim2018_COI	C22	1	BA83971_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Cjap_Sim2018_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83971_Esin_And2018_Cyb	C22	1	BA83971_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Esin_And2018_Cyb	C22	Cytb	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	ACCCCTCCTCATATCCAACCA	145.78376
BA83971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.92344	WGS84	BA83971	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83971_Mgig_Bot2012_COI	C22	1	BA83971_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83971	Sample	BA83971	targeted	Absent	Sample	Mgig_Bot2012_COI	C22	COI	45	BA83971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C22	TCTTATTCGTTGGAGACTTTATAACCCT	145.78376
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83972_Cmae_Bot2011_COI	C23	1	BA83972_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Cmae_Bot2011_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	ATGAACAGTCTATCCTCCTTTAG	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83972_Hsan_Wil2021_COI	C23	1	BA83972_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Hsan_Wil2021_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	CCTGGGCCGGTATAGTAGGT	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83972_Mros_Gib2022_COI	C23	1	BA83972_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Mros_Gib2022_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83972_Msal_Bot2012_COI	C23	1	BA83972_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Msal_Bot2012_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	GYTAGTTCCRATGATGTTAGCTG	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83972_Pvir_Dia2013_COI	C23	1	BA83972_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Pvir_Dia2013_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	CTTAGTGGCATTAATTCGDAATCC	145.78649
BA83972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91376	WGS84	BA83972	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83972_Rhar_Sim2018_COI	C23	1	BA83972_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83972	Sample	BA83972	targeted	Absent	Sample	Rhar_Sim2018_COI	C23	COI	45	BA83972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C23	CTGCTGCTATTGCTCATG	145.78649
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83974_Aamu_Bax2006_COI	C28	1	BA83974_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Aamu_Bax2006_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	GCACAACCGGGATCTTTACTTC	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83974_Pcan_Bot2011_IGS	C28	1	BA83974_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Pcan_Bot2011_IGS	C28	IGS	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	CGTAATCCTCAGTACTGGCTA	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83974_Pper_Dia2013_COI	C28	1	BA83974_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Pper_Dia2013_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	CTTAGTGGCATTAATTCGDAATCC	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83974_Sspa_Oph2007_COI	C28	1	BA83974_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Sspa_Oph2007_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	GAAGGCCTTCCGATGATC	145.77386
BA83974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.94644	WGS84	BA83974	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83974_Upin_Bot2011_COI	C28	1	BA83974_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83974	Sample	BA83974	targeted	Absent	Sample	Upin_Bot2011_COI	C28	COI	45	BA83974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C28	CTTTAATTACAGCGTTTTTATTGTTGT	145.77386
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83975_Cjap_Sim2018_COI	C29	1	BA83975_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Cjap_Sim2018_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83975_Esin_And2018_Cyb	C29	1	BA83975_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Esin_And2018_Cyb	C29	Cytb	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	ACCCCTCCTCATATCCAACCA	145.78312
BA83975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.93245	WGS84	BA83975	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83975_Mgig_Bot2012_COI	C29	1	BA83975_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83975	Sample	BA83975	targeted	Absent	Sample	Mgig_Bot2012_COI	C29	COI	45	BA83975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C29	TCTTATTCGTTGGAGACTTTATAACCCT	145.78312
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83976_Cmae_Bot2011_COI	C30	1	BA83976_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Cmae_Bot2011_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	ATGAACAGTCTATCCTCCTTTAG	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83976_Hsan_Wil2021_COI	C30	1	BA83976_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Hsan_Wil2021_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	CCTGGGCCGGTATAGTAGGT	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83976_Mros_Gib2022_COI	C30	1	BA83976_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Mros_Gib2022_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83976_Msal_Bot2012_COI	C30	1	BA83976_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Msal_Bot2012_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	GYTAGTTCCRATGATGTTAGCTG	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83976_Pvir_Dia2013_COI	C30	1	BA83976_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Pvir_Dia2013_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	CTTAGTGGCATTAATTCGDAATCC	145.7845
BA83976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-16.91682	WGS84	BA83976	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Cairns_1	26/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83976_Rhar_Sim2018_COI	C30	1	BA83976_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83976	Sample	BA83976	targeted	Absent	Sample	Rhar_Sim2018_COI	C30	COI	45	BA83976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	C30	CTGCTGCTATTGCTCATG	145.7845
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83978_Aamu_Bax2006_COI	A86	1	BA83978_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Aamu_Bax2006_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	GCACAACCGGGATCTTTACTTC	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83978_Pcan_Bot2011_IGS	A86	1	BA83978_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Pcan_Bot2011_IGS	A86	IGS	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	CGTAATCCTCAGTACTGGCTA	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83978_Pper_Dia2013_COI	A86	1	BA83978_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Pper_Dia2013_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	CTTAGTGGCATTAATTCGDAATCC	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83978_Sspa_Oph2007_COI	A86	1	BA83978_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Sspa_Oph2007_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	GAAGGCCTTCCGATGATC	138.5217
BA83978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8106	WGS84	BA83978	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83978_Upin_Bot2011_COI	A86	1	BA83978_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83978	Sample	BA83978	targeted	Absent	Sample	Upin_Bot2011_COI	A86	COI	45	BA83978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A86	CTTTAATTACAGCGTTTTTATTGTTGT	138.5217
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83979_Cjap_Sim2018_COI	A87	1	BA83979_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Cjap_Sim2018_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83979_Esin_And2018_Cyb	A87	1	BA83979_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Esin_And2018_Cyb	A87	Cytb	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	ACCCCTCCTCATATCCAACCA	138.51686
BA83979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.80852	WGS84	BA83979	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83979_Mgig_Bot2012_COI	A87	1	BA83979_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83979	Sample	BA83979	targeted	Absent	Sample	Mgig_Bot2012_COI	A87	COI	45	BA83979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A87	TCTTATTCGTTGGAGACTTTATAACCCT	138.51686
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83981_Aamu_Bax2006_COI	A89	1	BA83981_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Aamu_Bax2006_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	GCACAACCGGGATCTTTACTTC	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83981_Pcan_Bot2011_IGS	A89	1	BA83981_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Pcan_Bot2011_IGS	A89	IGS	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	CGTAATCCTCAGTACTGGCTA	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83981_Pper_Dia2013_COI	A89	1	BA83981_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Pper_Dia2013_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	CTTAGTGGCATTAATTCGDAATCC	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83981_Sspa_Oph2007_COI	A89	1	BA83981_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Sspa_Oph2007_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	GAAGGCCTTCCGATGATC	138.52362
BA83981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81096	WGS84	BA83981	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83981_Upin_Bot2011_COI	A89	1	BA83981_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83981	Sample	BA83981	targeted	Absent	Sample	Upin_Bot2011_COI	A89	COI	45	BA83981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A89	CTTTAATTACAGCGTTTTTATTGTTGT	138.52362
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83982_Cjap_Sim2018_COI	A90	1	BA83982_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Cjap_Sim2018_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83982_Esin_And2018_Cyb	A90	1	BA83982_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Esin_And2018_Cyb	A90	Cytb	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	ACCCCTCCTCATATCCAACCA	138.51743
BA83982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.8084	WGS84	BA83982	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83982_Mgig_Bot2012_COI	A90	1	BA83982_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83982	Sample	BA83982	targeted	Absent	Sample	Mgig_Bot2012_COI	A90	COI	45	BA83982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A90	TCTTATTCGTTGGAGACTTTATAACCCT	138.51743
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.347	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83983_Cmae_Bot2011_COI	A91	1	BA83983_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Present	Sample	Cmae_Bot2011_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	ATGAACAGTCTATCCTCCTTTAG	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83983_Hsan_Wil2021_COI	A91	1	BA83983_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Hsan_Wil2021_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	CCTGGGCCGGTATAGTAGGT	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83983_Mros_Gib2022_COI	A91	1	BA83983_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Mros_Gib2022_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83983_Msal_Bot2012_COI	A91	1	BA83983_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Msal_Bot2012_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	GYTAGTTCCRATGATGTTAGCTG	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83983_Pvir_Dia2013_COI	A91	1	BA83983_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Pvir_Dia2013_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	CTTAGTGGCATTAATTCGDAATCC	138.52058
BA83983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81079	WGS84	BA83983	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83983_Rhar_Sim2018_COI	A91	1	BA83983_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83983	Sample	BA83983	targeted	Absent	Sample	Rhar_Sim2018_COI	A91	COI	45	BA83983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A91	CTGCTGCTATTGCTCATG	138.52058
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA83985_Aamu_Bax2006_COI	A93	1	BA83985_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Aamu_Bax2006_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	GCACAACCGGGATCTTTACTTC	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BA83985_Pcan_Bot2011_IGS	A93	1	BA83985_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Pcan_Bot2011_IGS	A93	IGS	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	CGTAATCCTCAGTACTGGCTA	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA83985_Pper_Dia2013_COI	A93	1	BA83985_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Pper_Dia2013_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	CTTAGTGGCATTAATTCGDAATCC	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA83985_Sspa_Oph2007_COI	A93	1	BA83985_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Sspa_Oph2007_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	GAAGGCCTTCCGATGATC	138.52075
BA83985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81081	WGS84	BA83985	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA83985_Upin_Bot2011_COI	A93	1	BA83985_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83985	Sample	BA83985	targeted	Absent	Sample	Upin_Bot2011_COI	A93	COI	45	BA83985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A93	CTTTAATTACAGCGTTTTTATTGTTGT	138.52075
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA83986_Cjap_Sim2018_COI	A94	1	BA83986_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Cjap_Sim2018_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA83986_Esin_And2018_Cyb	A94	1	BA83986_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Esin_And2018_Cyb	A94	Cytb	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	ACCCCTCCTCATATCCAACCA	138.52268
BA83986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81101	WGS84	BA83986	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BA83986_Mgig_Bot2012_COI	A94	1	BA83986_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83986	Sample	BA83986	targeted	Absent	Sample	Mgig_Bot2012_COI	A94	COI	45	BA83986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A94	TCTTATTCGTTGGAGACTTTATAACCCT	138.52268
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA83987_Cmae_Bot2011_COI	A95	1	BA83987_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Cmae_Bot2011_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	ATGAACAGTCTATCCTCCTTTAG	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA83987_Hsan_Wil2021_COI	A95	1	BA83987_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Hsan_Wil2021_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	CCTGGGCCGGTATAGTAGGT	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA83987_Mros_Gib2022_COI	A95	1	BA83987_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Mros_Gib2022_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BA83987_Msal_Bot2012_COI	A95	1	BA83987_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Msal_Bot2012_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	GYTAGTTCCRATGATGTTAGCTG	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA83987_Pvir_Dia2013_COI	A95	1	BA83987_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Pvir_Dia2013_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	CTTAGTGGCATTAATTCGDAATCC	138.52086
BA83987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05433	-34.81037	WGS84	BA83987	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Expt_4	27/03/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA83987_Rhar_Sim2018_COI	A95	1	BA83987_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA83987	Sample	BA83987	targeted	Absent	Sample	Rhar_Sim2018_COI	A95	COI	45	BA83987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	A95	CTGCTGCTATTGCTCATG	138.52086
BA87563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.47405	WGS84	BA87563	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA87563_Pper_Dia2013_COI	D4	1	BA87563_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87563	Sample	BA87563	targeted	Absent	Sample	Pper_Dia2013_COI	D4	COI	45	BA87563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D4	CTTAGTGGCATTAATTCGDAATCC	130.84495
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA87564_Cjap_Sim2018_COI	D5	1	BA87564_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Cjap_Sim2018_COI	D5	COI	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85486
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA87564_Esin_And2018_Cyb	D5	1	BA87564_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Esin_And2018_Cyb	D5	Cytb	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	ACCCCTCCTCATATCCAACCA	130.85486
BA87564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.4477	WGS84	BA87564	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA87564_Mjap_Gib2022_COI	D5	1	BA87564_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87564	Sample	BA87564	targeted	Absent	Sample	Mjap_Gib2022_COI	D5	COI	45	BA87564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D5	AATTTAATTGTGACTAGGCATGGTCTG	130.85486
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA87565_Hsan_Wil2021_COI	D6	1	BA87565_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Hsan_Wil2021_COI	D6	COI	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	CCTGGGCCGGTATAGTAGGT	130.87387
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA87565_Mros_Gib2022_COI	D6	1	BA87565_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Mros_Gib2022_COI	D6	COI	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87387
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA87565_Pvir_Dia2013_COI	D6	1	BA87565_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Pvir_Dia2013_COI	D6	COI	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	CTTAGTGGCATTAATTCGDAATCC	130.87387
BA87565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48941	WGS84	BA87565	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA87565_Rhar_Sim2018_COI	D6	1	BA87565_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87565	Sample	BA87565	targeted	Absent	Sample	Rhar_Sim2018_COI	D6	COI	45	BA87565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D6	CTGCTGCTATTGCTCATG	130.87387
BA87567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.48861	WGS84	BA87567	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BA87567_Pper_Dia2013_COI	D11	1	BA87567_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87567	Sample	BA87567	targeted	Absent	Sample	Pper_Dia2013_COI	D11	COI	45	BA87567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D11	CTTAGTGGCATTAATTCGDAATCC	130.87453
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BA87568_Cjap_Sim2018_COI	D12	1	BA87568_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Cjap_Sim2018_COI	D12	COI	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.8249
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BA87568_Esin_And2018_Cyb	D12	1	BA87568_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Esin_And2018_Cyb	D12	Cytb	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	ACCCCTCCTCATATCCAACCA	130.8249
BA87568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42912	WGS84	BA87568	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BA87568_Mjap_Gib2022_COI	D12	1	BA87568_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87568	Sample	BA87568	targeted	Absent	Sample	Mjap_Gib2022_COI	D12	COI	45	BA87568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D12	AATTTAATTGTGACTAGGCATGGTCTG	130.8249
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BA87569_Hsan_Wil2021_COI	D22	1	BA87569_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Hsan_Wil2021_COI	D22	COI	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	CCTGGGCCGGTATAGTAGGT	130.82694
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BA87569_Mros_Gib2022_COI	D22	1	BA87569_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Mros_Gib2022_COI	D22	COI	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.82694
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BA87569_Pvir_Dia2013_COI	D22	1	BA87569_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Pvir_Dia2013_COI	D22	COI	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	CTTAGTGGCATTAATTCGDAATCC	130.82694
BA87569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05498	-12.42706	WGS84	BA87569	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Marine_Pests_Testing-Darwin_1	29/04/2015	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BA87569_Rhar_Sim2018_COI	D22	1	BA87569_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA87569	Sample	BA87569	targeted	Absent	Sample	Rhar_Sim2018_COI	D22	COI	45	BA87569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	D22	CTGCTGCTATTGCTCATG	130.82694
BA92914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77559	WGS84	BA92914	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.79851913	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92914_Sspa_Oph2007_COI	NP17	1	BA92914_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92914	Sample	BA92914	targeted	Present	Sample	Sspa_Oph2007_COI	NP17	COI	45	BA92914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP17	GAAGGCCTTCCGATGATC	138.48343
BA92918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77492	WGS84	BA92918	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.70132637	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92918_Sspa_Oph2007_COI	NP8	1	BA92918_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92918	Sample	BA92918	targeted	Present	Sample	Sspa_Oph2007_COI	NP8	COI	45	BA92918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP8	GAAGGCCTTCCGATGATC	138.48367
BA92925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77622	WGS84	BA92925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.82267761	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92925_Sspa_Oph2007_COI	NP4	1	BA92925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92925	Sample	BA92925	targeted	Present	Sample	Sspa_Oph2007_COI	NP4	COI	45	BA92925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP4	GAAGGCCTTCCGATGATC	138.48279
BA92929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77671	WGS84	BA92929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.4855423	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92929_Sspa_Oph2007_COI	NP1	1	BA92929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA92929	Sample	BA92929	targeted	Present	Sample	Sspa_Oph2007_COI	NP1	COI	45	BA92929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP1	GAAGGCCTTCCGATGATC	138.48253
BA92936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77468	WGS84	BA92936	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.37593651	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA92936_Sspa_Oph2007_COI	NP11	1	BA92936_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92936	Sample	BA92936	targeted	Present	Sample	Sspa_Oph2007_COI	NP11	COI	45	BA92936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP11	GAAGGCCTTCCGATGATC	138.484
BA92949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05588	-34.77618	WGS84	BA92949	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.921	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_9	16/06/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA92949_Cmae_Bot2011_COI	NP15	1	BA92949_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BA92949	Sample	BA92949	targeted	Present	Sample	Cmae_Bot2011_COI	NP15	COI	45	BA92949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NP15	ATGAACAGTCTATCCTCCTTTAG	138.48305
BA99029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84309	WGS84	BA99029	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.692	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99029_Aamu_Bax2006_COI	M17	1	BA99029_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99029	Sample	BA99029	targeted	Present	Sample	Aamu_Bax2006_COI	M17	COI	45	BA99029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M17	GCACAACCGGGATCTTTACTTC	144.93014
BA99030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.84208	WGS84	BA99030	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.53	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BA99030_Cmae_Bot2011_COI	M18	1	BA99030_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99030	Sample	BA99030	targeted	Present	Sample	Cmae_Bot2011_COI	M18	COI	45	BA99030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M18	ATGAACAGTCTATCCTCCTTTAG	144.92133
BA99032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.83866	WGS84	BA99032	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.29	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99032_Aamu_Bax2006_COI	M29	1	BA99032_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99032	Sample	BA99032	targeted	Present	Sample	Aamu_Bax2006_COI	M29	COI	45	BA99032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M29	GCACAACCGGGATCTTTACTTC	144.89674
BA99036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.81933	WGS84	BA99036	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.103	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BA99036_Aamu_Bax2006_COI	M6	1	BA99036_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99036	Sample	BA99036	targeted	Present	Sample	Aamu_Bax2006_COI	M6	COI	45	BA99036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M6	GCACAACCGGGATCTTTACTTC	144.91226
BA99036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.81933	WGS84	BA99036	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.4280014	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BA99036_Sspa_Oph2007_COI	M6	1	BA99036_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99036	Sample	BA99036	targeted	Present	Sample	Sspa_Oph2007_COI	M6	COI	45	BA99036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M6	GAAGGCCTTCCGATGATC	144.91226
BA99036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05679	-37.81933	WGS84	BA99036	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.16	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_1	7/10/2015	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BA99036_Upin_Bot2011_COI	M6	1	BA99036_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BA99036	Sample	BA99036	targeted	Present	Sample	Upin_Bot2011_COI	M6	COI	45	BA99036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M6	CTTTAATTACAGCGTTTTTATTGTTGT	144.91226
BB10184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81054	WGS84	BB10184	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.109	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10184_Cmae_Bot2011_COI	AS3	1	BB10184_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10184	Sample	BB10184	targeted	Present	Sample	Cmae_Bot2011_COI	AS3	COI	45	BB10184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS3	ATGAACAGTCTATCCTCCTTTAG	138.52101
BB10188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.8096	WGS84	BB10188	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.498	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10188_Cmae_Bot2011_COI	AS7	1	BB10188_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10188	Sample	BB10188	targeted	Present	Sample	Cmae_Bot2011_COI	AS7	COI	45	BB10188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS7	ATGAACAGTCTATCCTCCTTTAG	138.51504
BB10191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.81513	WGS84	BB10191	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.471	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10191_Cmae_Bot2011_COI	AS10	1	BB10191_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10191	Sample	BB10191	targeted	Present	Sample	Cmae_Bot2011_COI	AS10	COI	45	BB10191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS10	ATGAACAGTCTATCCTCCTTTAG	138.51274
BB10195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.82254	WGS84	BB10195	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.017	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10195_Cmae_Bot2011_COI	AS14	1	BB10195_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10195	Sample	BB10195	targeted	Present	Sample	Cmae_Bot2011_COI	AS14	COI	45	BB10195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS14	ATGAACAGTCTATCCTCCTTTAG	138.50977
BB10199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05838	-34.82502	WGS84	BB10199	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.448	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_12	25/09/2015	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB10199_Cmae_Bot2011_COI	AS18	1	BB10199_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB10199	Sample	BB10199	targeted	Present	Sample	Cmae_Bot2011_COI	AS18	COI	45	BB10199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS18	ATGAACAGTCTATCCTCCTTTAG	138.50813
BB12680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87368	WGS84	BB12680	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	23.924	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12680_Mgig_Bot2012_COI	H38	1	BB12680_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12680	Sample	BB12680	targeted	Present	Sample	Mgig_Bot2012_COI	H38	COI	45	BB12680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H38	TCTTATTCGTTGGAGACTTTATAACCCT	147.33723
BB12683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87437	WGS84	BB12683	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.177	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB12683_Aamu_Bax2006_COI	H41	1	BB12683_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12683	Sample	BB12683	targeted	Present	Sample	Aamu_Bax2006_COI	H41	COI	45	BB12683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H41	GCACAACCGGGATCTTTACTTC	147.33773
BB12684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87288	WGS84	BB12684	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.023	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12684_Mgig_Bot2012_COI	H42	1	BB12684_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12684	Sample	BB12684	targeted	Present	Sample	Mgig_Bot2012_COI	H42	COI	45	BB12684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H42	TCTTATTCGTTGGAGACTTTATAACCCT	147.36502
BB12688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88252	WGS84	BB12688	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.077	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12688_Mgig_Bot2012_COI	H46	1	BB12688_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12688	Sample	BB12688	targeted	Present	Sample	Mgig_Bot2012_COI	H46	COI	45	BB12688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H46	TCTTATTCGTTGGAGACTTTATAACCCT	147.34067
BB12689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.87697	WGS84	BB12689	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.983	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12689_Cmae_Bot2011_COI	H47	1	BB12689_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12689	Sample	BB12689	targeted	Present	Sample	Cmae_Bot2011_COI	H47	COI	45	BB12689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H47	ATGAACAGTCTATCCTCCTTTAG	147.34149
BB12691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.85057	WGS84	BB12691	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.468	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12691_Mgig_Bot2012_COI	H49	1	BB12691_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB12691	Sample	BB12691	targeted	Present	Sample	Mgig_Bot2012_COI	H49	COI	45	BB12691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H49	TCTTATTCGTTGGAGACTTTATAACCCT	147.35568
BB12695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88468	WGS84	BB12695	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.336	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB12695_Mgig_Bot2012_COI	H53	1	BB12695_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12695	Sample	BB12695	targeted	Present	Sample	Mgig_Bot2012_COI	H53	COI	45	BB12695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H53	TCTTATTCGTTGGAGACTTTATAACCCT	147.33773
BB12696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05883	-42.88292	WGS84	BB12696	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.259	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Hobart_2	1/12/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB12696_Cmae_Bot2011_COI	H54	1	BB12696_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB12696	Sample	BB12696	targeted	Present	Sample	Cmae_Bot2011_COI	H54	COI	45	BB12696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	H54	ATGAACAGTCTATCCTCCTTTAG	147.33613
BB16133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.83387	WGS84	BB16133	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.16199875	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB16133_Sspa_Oph2007_COI	AS41	1	BB16133_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16133	Sample	BB16133	targeted	Present	Sample	Sspa_Oph2007_COI	AS41	COI	45	BB16133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS41	GAAGGCCTTCCGATGATC	138.50797
BB16138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05979	-34.82417	WGS84	BB16138	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.864	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Expt_14	3/03/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB16138_Mgig_Bot2012_COI	AS46	1	BB16138_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BB16138	Sample	BB16138	targeted	Present	Sample	Mgig_Bot2012_COI	AS46	COI	45	BB16138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	AS46	TCTTATTCGTTGGAGACTTTATAACCCT	138.51043
BB17780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84316	WGS84	BB17780	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.84	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17780_Mgig_Bot2012_COI	S37	1	BB17780_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17780	Sample	BB17780	targeted	Present	Sample	Mgig_Bot2012_COI	S37	COI	45	BB17780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S37	TCTTATTCGTTGGAGACTTTATAACCCT	151.27762
BB17784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84694	WGS84	BB17784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.071	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17784_Mgig_Bot2012_COI	S41	1	BB17784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17784	Sample	BB17784	targeted	Present	Sample	Mgig_Bot2012_COI	S41	COI	45	BB17784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S41	TCTTATTCGTTGGAGACTTTATAACCCT	151.27578
BB17788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84617	WGS84	BB17788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.067	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17788_Mgig_Bot2012_COI	S45	1	BB17788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17788	Sample	BB17788	targeted	Present	Sample	Mgig_Bot2012_COI	S45	COI	45	BB17788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S45	TCTTATTCGTTGGAGACTTTATAACCCT	151.27687
BB17791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84623	WGS84	BB17791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.887	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17791_Mgig_Bot2012_COI	S48	1	BB17791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17791	Sample	BB17791	targeted	Present	Sample	Mgig_Bot2012_COI	S48	COI	45	BB17791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S48	TCTTATTCGTTGGAGACTTTATAACCCT	151.27676
BB17795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_05996	-33.84824	WGS84	BB17795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	24.372	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Sydney_2	25/02/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB17795_Mgig_Bot2012_COI	S52	1	BB17795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB17795	Sample	BB17795	targeted	Present	Sample	Mgig_Bot2012_COI	S52	COI	45	BB17795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S52	TCTTATTCGTTGGAGACTTTATAACCCT	151.27266
BB32902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01817	WGS84	BB32902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32902_Aamu_Bax2006_COI	PS-A01	1	BB32902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32902	Sample	BB32902	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A01	COI	45	BB32902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A01	GCACAACCGGGATCTTTACTTC	137.60078
BB32902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01817	WGS84	BB32902	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32902_Pcan_Bot2011_IGS	PS-A01	1	BB32902_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32902	Sample	BB32902	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A01	IGS	45	BB32902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A01	CGTAATCCTCAGTACTGGCTA	137.60078
BB32902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01817	WGS84	BB32902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.6060009	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32902_Sspa_Oph2007_COI	PS-A01	1	BB32902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32902	Sample	BB32902	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A01	COI	45	BB32902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A01	GAAGGCCTTCCGATGATC	137.60078
BB32902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01817	WGS84	BB32902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32902_Upin_Bot2011_COI	PS-A01	1	BB32902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32902	Sample	BB32902	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A01	COI	45	BB32902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A01	CTTTAATTACAGCGTTTTTATTGTTGT	137.60078
BB32903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01742	WGS84	BB32903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32903_Mgig_Bot2012_COI	PS-A02	1	BB32903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32903	Sample	BB32903	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A02	COI	45	BB32903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A02	TCTTATTCGTTGGAGACTTTATAACCCT	137.59663
BB32904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01922	WGS84	BB32904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32904_Cmae_Bot2011_COI	PS-A03	1	BB32904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32904	Sample	BB32904	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A03	COI	45	BB32904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A03	ATGAACAGTCTATCCTCCTTTAG	137.59862
BB32904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01922	WGS84	BB32904	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32904_Msal_Bot2012_COI	PS-A03	1	BB32904_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32904	Sample	BB32904	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A03	COI	45	BB32904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A03	GYTAGTTCCRATGATGTTAGCTG	137.59862
BB32906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03257	WGS84	BB32906	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32906_Aamu_Bax2006_COI	PS-A05	1	BB32906_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32906	Sample	BB32906	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A05	COI	45	BB32906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A05	GCACAACCGGGATCTTTACTTC	137.60012
BB32906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03257	WGS84	BB32906	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32906_Pcan_Bot2011_IGS	PS-A05	1	BB32906_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32906	Sample	BB32906	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A05	IGS	45	BB32906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A05	CGTAATCCTCAGTACTGGCTA	137.60012
BB32906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03257	WGS84	BB32906	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.77799988	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32906_Sspa_Oph2007_COI	PS-A05	1	BB32906_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32906	Sample	BB32906	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A05	COI	45	BB32906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A05	GAAGGCCTTCCGATGATC	137.60012
BB32906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03257	WGS84	BB32906	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32906_Upin_Bot2011_COI	PS-A05	1	BB32906_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32906	Sample	BB32906	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A05	COI	45	BB32906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A05	CTTTAATTACAGCGTTTTTATTGTTGT	137.60012
BB32907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03665	WGS84	BB32907	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32907_Mgig_Bot2012_COI	PS-A06	1	BB32907_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32907	Sample	BB32907	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A06	COI	45	BB32907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A06	TCTTATTCGTTGGAGACTTTATAACCCT	137.59798
BB32908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03939	WGS84	BB32908	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32908_Cmae_Bot2011_COI	PS-A07	1	BB32908_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32908	Sample	BB32908	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A07	COI	45	BB32908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A07	ATGAACAGTCTATCCTCCTTTAG	137.59628
BB32908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03939	WGS84	BB32908	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32908_Msal_Bot2012_COI	PS-A07	1	BB32908_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32908	Sample	BB32908	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A07	COI	45	BB32908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A07	GYTAGTTCCRATGATGTTAGCTG	137.59628
BB32910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03907	WGS84	BB32910	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32910_Mgig_Bot2012_COI	PS-A09	1	BB32910_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32910	Sample	BB32910	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A09	COI	45	BB32910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A09	TCTTATTCGTTGGAGACTTTATAACCCT	137.59498
BB32911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03909	WGS84	BB32911	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32911_Cmae_Bot2011_COI	PS-A10	1	BB32911_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32911	Sample	BB32911	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A10	COI	45	BB32911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A10	ATGAACAGTCTATCCTCCTTTAG	137.59297
BB32911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03909	WGS84	BB32911	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32911_Msal_Bot2012_COI	PS-A10	1	BB32911_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32911	Sample	BB32911	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A10	COI	45	BB32911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A10	GYTAGTTCCRATGATGTTAGCTG	137.59297
BB32913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01847	WGS84	BB32913	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32913_Aamu_Bax2006_COI	PS-A12	1	BB32913_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32913	Sample	BB32913	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A12	COI	45	BB32913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A12	GCACAACCGGGATCTTTACTTC	137.59956
BB32913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01847	WGS84	BB32913	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32913_Pcan_Bot2011_IGS	PS-A12	1	BB32913_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32913	Sample	BB32913	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A12	IGS	45	BB32913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A12	CGTAATCCTCAGTACTGGCTA	137.59956
BB32913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01847	WGS84	BB32913	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.44000053	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32913_Sspa_Oph2007_COI	PS-A12	1	BB32913_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32913	Sample	BB32913	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A12	COI	45	BB32913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A12	GAAGGCCTTCCGATGATC	137.59956
BB32913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01847	WGS84	BB32913	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32913_Upin_Bot2011_COI	PS-A12	1	BB32913_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32913	Sample	BB32913	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A12	COI	45	BB32913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A12	CTTTAATTACAGCGTTTTTATTGTTGT	137.59956
BB32914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01807	WGS84	BB32914	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32914_Mgig_Bot2012_COI	PS-A13	1	BB32914_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32914	Sample	BB32914	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A13	COI	45	BB32914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A13	TCTTATTCGTTGGAGACTTTATAACCCT	137.59817
BB32915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01772	WGS84	BB32915	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32915_Cmae_Bot2011_COI	PS-A14	1	BB32915_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32915	Sample	BB32915	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A14	COI	45	BB32915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A14	ATGAACAGTCTATCCTCCTTTAG	137.59601
BB32915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01772	WGS84	BB32915	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32915_Msal_Bot2012_COI	PS-A14	1	BB32915_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32915	Sample	BB32915	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A14	COI	45	BB32915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A14	GYTAGTTCCRATGATGTTAGCTG	137.59601
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01982	WGS84	BB32917	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32917_Aamu_Bax2006_COI	PS-A16	1	BB32917_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	GCACAACCGGGATCTTTACTTC	137.59981
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01982	WGS84	BB32917	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32917_Pcan_Bot2011_IGS	PS-A16	1	BB32917_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A16	IGS	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	CGTAATCCTCAGTACTGGCTA	137.59981
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01982	WGS84	BB32917	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.0529995	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32917_Sspa_Oph2007_COI	PS-A16	1	BB32917_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	GAAGGCCTTCCGATGATC	137.59981
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.01982	WGS84	BB32917	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32917_Upin_Bot2011_COI	PS-A16	1	BB32917_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	CTTTAATTACAGCGTTTTTATTGTTGT	137.59981
BB32917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.01982	WGS84	BB32917	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32917_Vgib_Wil2025_COI	PS-A16	1	BB32917_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32917	Sample	BB32917	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A16	COI	45	BB32917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A16	CCCCTCTTTACAAGTTCTTTAGGGT	137.59981
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.02044	WGS84	BB32918	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32918_Asen_Wil2025_COI	PS-A17	1	BB32918_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	GTTGTTGTTACAACACATGCTCTTTTG	137.60071
BB32918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02044	WGS84	BB32918	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32918_Mgig_Bot2012_COI	PS-A17	1	BB32918_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32918	Sample	BB32918	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A17	COI	45	BB32918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A17	TCTTATTCGTTGGAGACTTTATAACCCT	137.60071
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02985	WGS84	BB32919	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32919_Cmae_Bot2011_COI	PS-A18	1	BB32919_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	ATGAACAGTCTATCCTCCTTTAG	137.60018
BB32919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.02985	WGS84	BB32919	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32919_Msal_Bot2012_COI	PS-A18	1	BB32919_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32919	Sample	BB32919	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A18	COI	45	BB32919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A18	GYTAGTTCCRATGATGTTAGCTG	137.60018
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03113	WGS84	BB32920	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32920_Aamu_Bax2006_COI	PS-A19	1	BB32920_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	GCACAACCGGGATCTTTACTTC	137.60008
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03113	WGS84	BB32920	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32920_Pcan_Bot2011_IGS	PS-A19	1	BB32920_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A19	IGS	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	CGTAATCCTCAGTACTGGCTA	137.60008
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03113	WGS84	BB32920	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.97700119	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32920_Sspa_Oph2007_COI	PS-A19	1	BB32920_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	GAAGGCCTTCCGATGATC	137.60008
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03113	WGS84	BB32920	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32920_Upin_Bot2011_COI	PS-A19	1	BB32920_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	CTTTAATTACAGCGTTTTTATTGTTGT	137.60008
BB32920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03113	WGS84	BB32920	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32920_Vgib_Wil2025_COI	PS-A19	1	BB32920_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32920	Sample	BB32920	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A19	COI	45	BB32920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A19	CCCCTCTTTACAAGTTCTTTAGGGT	137.60008
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03389	WGS84	BB32921	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32921_Asen_Wil2025_COI	PS-A20	1	BB32921_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	GTTGTTGTTACAACACATGCTCTTTTG	137.60019
BB32921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03389	WGS84	BB32921	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32921_Mgig_Bot2012_COI	PS-A20	1	BB32921_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32921	Sample	BB32921	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A20	COI	45	BB32921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A20	TCTTATTCGTTGGAGACTTTATAACCCT	137.60019
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03692	WGS84	BB32922	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32922_Cmae_Bot2011_COI	PS-A21	1	BB32922_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	ATGAACAGTCTATCCTCCTTTAG	137.59936
BB32922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03692	WGS84	BB32922	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32922_Msal_Bot2012_COI	PS-A21	1	BB32922_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32922	Sample	BB32922	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A21	COI	45	BB32922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A21	GYTAGTTCCRATGATGTTAGCTG	137.59936
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03825	WGS84	BB32924	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32924_Aamu_Bax2006_COI	PS-A23	1	BB32924_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	GCACAACCGGGATCTTTACTTC	137.59652
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03825	WGS84	BB32924	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32924_Pcan_Bot2011_IGS	PS-A23	1	BB32924_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A23	IGS	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	CGTAATCCTCAGTACTGGCTA	137.59652
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03825	WGS84	BB32924	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.94499969	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32924_Sspa_Oph2007_COI	PS-A23	1	BB32924_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	GAAGGCCTTCCGATGATC	137.59652
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03825	WGS84	BB32924	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32924_Upin_Bot2011_COI	PS-A23	1	BB32924_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	CTTTAATTACAGCGTTTTTATTGTTGT	137.59652
BB32924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03825	WGS84	BB32924	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32924_Vgib_Wil2025_COI	PS-A23	1	BB32924_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32924	Sample	BB32924	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A23	COI	45	BB32924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A23	CCCCTCTTTACAAGTTCTTTAGGGT	137.59652
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04269	WGS84	BB32925	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32925_Asen_Wil2025_COI	PS-A24	1	BB32925_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	GTTGTTGTTACAACACATGCTCTTTTG	137.59376
BB32925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04269	WGS84	BB32925	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32925_Mgig_Bot2012_COI	PS-A24	1	BB32925_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32925	Sample	BB32925	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A24	COI	45	BB32925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A24	TCTTATTCGTTGGAGACTTTATAACCCT	137.59376
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.0418	WGS84	BB32926	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32926_Cmae_Bot2011_COI	PS-A25	1	BB32926_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	ATGAACAGTCTATCCTCCTTTAG	137.59386
BB32926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.0418	WGS84	BB32926	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32926_Msal_Bot2012_COI	PS-A25	1	BB32926_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32926	Sample	BB32926	targeted	Absent	Sample	Msal_Bot2012_COI	PS-A25	COI	45	BB32926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A25	GYTAGTTCCRATGATGTTAGCTG	137.59386
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03928	WGS84	BB32928	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32928_Aamu_Bax2006_COI	PS-A27	1	BB32928_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	GCACAACCGGGATCTTTACTTC	137.59475
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03928	WGS84	BB32928	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32928_Pcan_Bot2011_IGS	PS-A27	1	BB32928_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A27	IGS	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	CGTAATCCTCAGTACTGGCTA	137.59475
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03928	WGS84	BB32928	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.09400177	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32928_Sspa_Oph2007_COI	PS-A27	1	BB32928_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	GAAGGCCTTCCGATGATC	137.59475
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.03928	WGS84	BB32928	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32928_Upin_Bot2011_COI	PS-A27	1	BB32928_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	CTTTAATTACAGCGTTTTTATTGTTGT	137.59475
BB32928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.03928	WGS84	BB32928	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32928_Vgib_Wil2025_COI	PS-A27	1	BB32928_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32928	Sample	BB32928	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A27	COI	45	BB32928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A27	CCCCTCTTTACAAGTTCTTTAGGGT	137.59475
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04087	WGS84	BB32929	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32929_Asen_Wil2025_COI	PS-A28	1	BB32929_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Asen_Wil2025_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	GTTGTTGTTACAACACATGCTCTTTTG	137.59434
BB32929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04087	WGS84	BB32929	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32929_Mgig_Bot2012_COI	PS-A28	1	BB32929_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32929	Sample	BB32929	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-A28	COI	45	BB32929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A28	TCTTATTCGTTGGAGACTTTATAACCCT	137.59434
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04053	WGS84	BB32931	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32931_Aamu_Bax2006_COI	PS-A30	1	BB32931_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	GCACAACCGGGATCTTTACTTC	137.59253
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04053	WGS84	BB32931	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32931_Pcan_Bot2011_IGS	PS-A30	1	BB32931_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-A30	IGS	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	CGTAATCCTCAGTACTGGCTA	137.59253
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04053	WGS84	BB32931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.92900085	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32931_Sspa_Oph2007_COI	PS-A30	1	BB32931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Present	Sample	Sspa_Oph2007_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	GAAGGCCTTCCGATGATC	137.59253
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.04053	WGS84	BB32931	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32931_Upin_Bot2011_COI	PS-A30	1	BB32931_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Upin_Bot2011_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	CTTTAATTACAGCGTTTTTATTGTTGT	137.59253
BB32931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-33.04053	WGS84	BB32931	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Whyalla	19/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32931_Vgib_Wil2025_COI	PS-A30	1	BB32931_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32931	Sample	BB32931	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-A30	COI	45	BB32931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-A30	CCCCTCTTTACAAGTTCTTTAGGGT	137.59253
BB32937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71952	WGS84	BB32937	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32937_Cmae_Bot2011_COI	PS-B01	1	BB32937_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32937	Sample	BB32937	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B01	COI	45	BB32937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B01	ATGAACAGTCTATCCTCCTTTAG	135.86182
BB32937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71952	WGS84	BB32937	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32937_Msal_Bot2012_COI	PS-B01	1	BB32937_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32937	Sample	BB32937	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B01	COI	45	BB32937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B01	GYTAGTTCCRATGATGTTAGCTG	135.86182
BB32939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71791	WGS84	BB32939	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32939_Aamu_Bax2006_COI	PS-B03	1	BB32939_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32939	Sample	BB32939	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B03	COI	45	BB32939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B03	GCACAACCGGGATCTTTACTTC	135.86262
BB32939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71791	WGS84	BB32939	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32939_Pcan_Bot2011_IGS	PS-B03	1	BB32939_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32939	Sample	BB32939	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B03	IGS	45	BB32939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B03	CGTAATCCTCAGTACTGGCTA	135.86262
BB32939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71791	WGS84	BB32939	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.14899826	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32939_Sspa_Oph2007_COI	PS-B03	1	BB32939_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32939	Sample	BB32939	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B03	COI	45	BB32939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B03	GAAGGCCTTCCGATGATC	135.86262
BB32939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71791	WGS84	BB32939	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32939_Upin_Bot2011_COI	PS-B03	1	BB32939_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32939	Sample	BB32939	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B03	COI	45	BB32939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B03	CTTTAATTACAGCGTTTTTATTGTTGT	135.86262
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71539	WGS84	BB32940	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32940_Cmae_Bot2011_COI	PS-B04	1	BB32940_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	ATGAACAGTCTATCCTCCTTTAG	135.86716
BB32940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71539	WGS84	BB32940	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32940_Msal_Bot2012_COI	PS-B04	1	BB32940_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32940	Sample	BB32940	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B04	COI	45	BB32940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B04	GYTAGTTCCRATGATGTTAGCTG	135.86716
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71875	WGS84	BB32942	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32942_Aamu_Bax2006_COI	PS-B06	1	BB32942_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	GCACAACCGGGATCTTTACTTC	135.87153
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71875	WGS84	BB32942	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32942_Pcan_Bot2011_IGS	PS-B06	1	BB32942_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B06	IGS	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	CGTAATCCTCAGTACTGGCTA	135.87153
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71875	WGS84	BB32942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32942_Sspa_Oph2007_COI	PS-B06	1	BB32942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	GAAGGCCTTCCGATGATC	135.87153
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71875	WGS84	BB32942	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32942_Upin_Bot2011_COI	PS-B06	1	BB32942_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	CTTTAATTACAGCGTTTTTATTGTTGT	135.87153
BB32942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71875	WGS84	BB32942	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32942_Vgib_Wil2025_COI	PS-B06	1	BB32942_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32942	Sample	BB32942	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B06	COI	45	BB32942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B06	CCCCTCTTTACAAGTTCTTTAGGGT	135.87153
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.72029	WGS84	BB32943	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32943_Asen_Wil2025_COI	PS-B07	1	BB32943_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	GTTGTTGTTACAACACATGCTCTTTTG	135.87318
BB32943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72029	WGS84	BB32943	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32943_Mgig_Bot2012_COI	PS-B07	1	BB32943_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32943	Sample	BB32943	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B07	COI	45	BB32943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B07	TCTTATTCGTTGGAGACTTTATAACCCT	135.87318
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71838	WGS84	BB32944	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32944_Cmae_Bot2011_COI	PS-B08	1	BB32944_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	ATGAACAGTCTATCCTCCTTTAG	135.87715
BB32944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71838	WGS84	BB32944	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32944_Msal_Bot2012_COI	PS-B08	1	BB32944_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32944	Sample	BB32944	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B08	COI	45	BB32944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B08	GYTAGTTCCRATGATGTTAGCTG	135.87715
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72483	WGS84	BB32946	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32946_Aamu_Bax2006_COI	PS-B10	1	BB32946_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	GCACAACCGGGATCTTTACTTC	135.88569
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72483	WGS84	BB32946	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32946_Pcan_Bot2011_IGS	PS-B10	1	BB32946_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B10	IGS	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	CGTAATCCTCAGTACTGGCTA	135.88569
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72483	WGS84	BB32946	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32946_Sspa_Oph2007_COI	PS-B10	1	BB32946_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	GAAGGCCTTCCGATGATC	135.88569
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72483	WGS84	BB32946	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32946_Upin_Bot2011_COI	PS-B10	1	BB32946_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	CTTTAATTACAGCGTTTTTATTGTTGT	135.88569
BB32946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.72483	WGS84	BB32946	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB32946_Vgib_Wil2025_COI	PS-B10	1	BB32946_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32946	Sample	BB32946	targeted	Absent	Sample	Vgib_Wil2025_COI	PS-B10	COI	45	BB32946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B10	CCCCTCTTTACAAGTTCTTTAGGGT	135.88569
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-34.71863	WGS84	BB32947	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB32947_Asen_Wil2025_COI	PS-B11	1	BB32947_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Asen_Wil2025_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	GTTGTTGTTACAACACATGCTCTTTTG	135.86087
BB32947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71863	WGS84	BB32947	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32947_Mgig_Bot2012_COI	PS-B11	1	BB32947_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32947	Sample	BB32947	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B11	COI	45	BB32947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B11	TCTTATTCGTTGGAGACTTTATAACCCT	135.86087
BB32948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72021	WGS84	BB32948	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32948_Cmae_Bot2011_COI	PS-B12	1	BB32948_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32948	Sample	BB32948	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B12	COI	45	BB32948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B12	ATGAACAGTCTATCCTCCTTTAG	135.8631
BB32948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72021	WGS84	BB32948	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32948_Msal_Bot2012_COI	PS-B12	1	BB32948_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32948	Sample	BB32948	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B12	COI	45	BB32948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B12	GYTAGTTCCRATGATGTTAGCTG	135.8631
BB32950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72022	WGS84	BB32950	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32950_Mgig_Bot2012_COI	PS-B14	1	BB32950_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32950	Sample	BB32950	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B14	COI	45	BB32950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B14	TCTTATTCGTTGGAGACTTTATAACCCT	135.86322
BB32951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71958	WGS84	BB32951	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32951_Cmae_Bot2011_COI	PS-B15	1	BB32951_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32951	Sample	BB32951	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B15	COI	45	BB32951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B15	ATGAACAGTCTATCCTCCTTTAG	135.86192
BB32951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71958	WGS84	BB32951	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32951_Msal_Bot2012_COI	PS-B15	1	BB32951_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32951	Sample	BB32951	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B15	COI	45	BB32951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B15	GYTAGTTCCRATGATGTTAGCTG	135.86192
BB32953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7164	WGS84	BB32953	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32953_Aamu_Bax2006_COI	PS-B17	1	BB32953_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32953	Sample	BB32953	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B17	COI	45	BB32953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B17	GCACAACCGGGATCTTTACTTC	135.86547
BB32953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7164	WGS84	BB32953	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32953_Pcan_Bot2011_IGS	PS-B17	1	BB32953_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32953	Sample	BB32953	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B17	IGS	45	BB32953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B17	CGTAATCCTCAGTACTGGCTA	135.86547
BB32953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7164	WGS84	BB32953	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32953_Sspa_Oph2007_COI	PS-B17	1	BB32953_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32953	Sample	BB32953	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B17	COI	45	BB32953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B17	GAAGGCCTTCCGATGATC	135.86547
BB32953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7164	WGS84	BB32953	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32953_Upin_Bot2011_COI	PS-B17	1	BB32953_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32953	Sample	BB32953	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B17	COI	45	BB32953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B17	CTTTAATTACAGCGTTTTTATTGTTGT	135.86547
BB32954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71403	WGS84	BB32954	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32954_Mgig_Bot2012_COI	PS-B18	1	BB32954_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32954	Sample	BB32954	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B18	COI	45	BB32954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B18	TCTTATTCGTTGGAGACTTTATAACCCT	135.86807
BB32955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71395	WGS84	BB32955	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32955_Cmae_Bot2011_COI	PS-B19	1	BB32955_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32955	Sample	BB32955	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B19	COI	45	BB32955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B19	ATGAACAGTCTATCCTCCTTTAG	135.87171
BB32955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71395	WGS84	BB32955	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32955_Msal_Bot2012_COI	PS-B19	1	BB32955_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32955	Sample	BB32955	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B19	COI	45	BB32955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B19	GYTAGTTCCRATGATGTTAGCTG	135.87171
BB32957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7175	WGS84	BB32957	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32957_Aamu_Bax2006_COI	PS-B21	1	BB32957_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32957	Sample	BB32957	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B21	COI	45	BB32957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B21	GCACAACCGGGATCTTTACTTC	135.87182
BB32957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7175	WGS84	BB32957	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32957_Pcan_Bot2011_IGS	PS-B21	1	BB32957_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32957	Sample	BB32957	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B21	IGS	45	BB32957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B21	CGTAATCCTCAGTACTGGCTA	135.87182
BB32957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7175	WGS84	BB32957	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32957_Sspa_Oph2007_COI	PS-B21	1	BB32957_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32957	Sample	BB32957	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B21	COI	45	BB32957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B21	GAAGGCCTTCCGATGATC	135.87182
BB32957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.7175	WGS84	BB32957	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32957_Upin_Bot2011_COI	PS-B21	1	BB32957_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32957	Sample	BB32957	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B21	COI	45	BB32957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B21	CTTTAATTACAGCGTTTTTATTGTTGT	135.87182
BB32958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72017	WGS84	BB32958	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32958_Mgig_Bot2012_COI	PS-B22	1	BB32958_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32958	Sample	BB32958	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B22	COI	45	BB32958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B22	TCTTATTCGTTGGAGACTTTATAACCCT	135.87103
BB32959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72081	WGS84	BB32959	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32959_Cmae_Bot2011_COI	PS-B23	1	BB32959_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32959	Sample	BB32959	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B23	COI	45	BB32959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B23	ATGAACAGTCTATCCTCCTTTAG	135.87177
BB32959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72081	WGS84	BB32959	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32959_Msal_Bot2012_COI	PS-B23	1	BB32959_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32959	Sample	BB32959	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B23	COI	45	BB32959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B23	GYTAGTTCCRATGATGTTAGCTG	135.87177
BB32960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71918	WGS84	BB32960	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32960_Aamu_Bax2006_COI	PS-B24	1	BB32960_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32960	Sample	BB32960	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B24	COI	45	BB32960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B24	GCACAACCGGGATCTTTACTTC	135.87416
BB32960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71918	WGS84	BB32960	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32960_Pcan_Bot2011_IGS	PS-B24	1	BB32960_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32960	Sample	BB32960	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B24	IGS	45	BB32960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B24	CGTAATCCTCAGTACTGGCTA	135.87416
BB32960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71918	WGS84	BB32960	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32960_Sspa_Oph2007_COI	PS-B24	1	BB32960_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32960	Sample	BB32960	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-B24	COI	45	BB32960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B24	GAAGGCCTTCCGATGATC	135.87416
BB32960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71918	WGS84	BB32960	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32960_Upin_Bot2011_COI	PS-B24	1	BB32960_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32960	Sample	BB32960	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B24	COI	45	BB32960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B24	CTTTAATTACAGCGTTTTTATTGTTGT	135.87416
BB32961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71855	WGS84	BB32961	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32961_Mgig_Bot2012_COI	PS-B25	1	BB32961_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32961	Sample	BB32961	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B25	COI	45	BB32961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B25	TCTTATTCGTTGGAGACTTTATAACCCT	135.87565
BB32962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71858	WGS84	BB32962	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32962_Cmae_Bot2011_COI	PS-B26	1	BB32962_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32962	Sample	BB32962	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B26	COI	45	BB32962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B26	ATGAACAGTCTATCCTCCTTTAG	135.87847
BB32962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.71858	WGS84	BB32962	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32962_Msal_Bot2012_COI	PS-B26	1	BB32962_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32962	Sample	BB32962	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B26	COI	45	BB32962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B26	GYTAGTTCCRATGATGTTAGCTG	135.87847
BB32964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.723	WGS84	BB32964	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32964_Aamu_Bax2006_COI	PS-B28	1	BB32964_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32964	Sample	BB32964	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-B28	COI	45	BB32964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B28	GCACAACCGGGATCTTTACTTC	135.88382
BB32964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.723	WGS84	BB32964	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32964_Pcan_Bot2011_IGS	PS-B28	1	BB32964_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32964	Sample	BB32964	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-B28	IGS	45	BB32964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B28	CGTAATCCTCAGTACTGGCTA	135.88382
BB32964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.723	WGS84	BB32964	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.59400177	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32964_Sspa_Oph2007_COI	PS-B28	1	BB32964_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32964	Sample	BB32964	targeted	Present	Sample	Sspa_Oph2007_COI	PS-B28	COI	45	BB32964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B28	GAAGGCCTTCCGATGATC	135.88382
BB32964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.723	WGS84	BB32964	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32964_Upin_Bot2011_COI	PS-B28	1	BB32964_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32964	Sample	BB32964	targeted	Absent	Sample	Upin_Bot2011_COI	PS-B28	COI	45	BB32964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B28	CTTTAATTACAGCGTTTTTATTGTTGT	135.88382
BB32965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72362	WGS84	BB32965	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32965_Mgig_Bot2012_COI	PS-B29	1	BB32965_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32965	Sample	BB32965	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-B29	COI	45	BB32965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B29	TCTTATTCGTTGGAGACTTTATAACCCT	135.88477
BB32966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72586	WGS84	BB32966	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32966_Cmae_Bot2011_COI	PS-B30	1	BB32966_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32966	Sample	BB32966	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-B30	COI	45	BB32966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B30	ATGAACAGTCTATCCTCCTTTAG	135.88634
BB32966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-34.72586	WGS84	BB32966	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Port_Lincoln	26/05/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32966_Msal_Bot2012_COI	PS-B30	1	BB32966_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32966	Sample	BB32966	targeted	Absent	Sample	Msal_Bot2012_COI	PS-B30	COI	45	BB32966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-B30	GYTAGTTCCRATGATGTTAGCTG	135.88634
BB32972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93052	WGS84	BB32972	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32972_Mgig_Bot2012_COI	PS-C01	1	BB32972_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32972	Sample	BB32972	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C01	COI	45	BB32972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C01	TCTTATTCGTTGGAGACTTTATAACCCT	137.61115
BB32973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93135	WGS84	BB32973	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32973_Cmae_Bot2011_COI	PS-C02	1	BB32973_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32973	Sample	BB32973	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C02	COI	45	BB32973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C02	ATGAACAGTCTATCCTCCTTTAG	137.60781
BB32973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93135	WGS84	BB32973	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32973_Msal_Bot2012_COI	PS-C02	1	BB32973_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32973	Sample	BB32973	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C02	COI	45	BB32973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C02	GYTAGTTCCRATGATGTTAGCTG	137.60781
BB32975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92373	WGS84	BB32975	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32975_Aamu_Bax2006_COI	PS-C04	1	BB32975_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32975	Sample	BB32975	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C04	COI	45	BB32975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C04	GCACAACCGGGATCTTTACTTC	137.61315
BB32975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92373	WGS84	BB32975	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32975_Pcan_Bot2011_IGS	PS-C04	1	BB32975_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32975	Sample	BB32975	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C04	IGS	45	BB32975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C04	CGTAATCCTCAGTACTGGCTA	137.61315
BB32975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92373	WGS84	BB32975	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32975_Sspa_Oph2007_COI	PS-C04	1	BB32975_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32975	Sample	BB32975	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C04	COI	45	BB32975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C04	GAAGGCCTTCCGATGATC	137.61315
BB32975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92373	WGS84	BB32975	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32975_Upin_Bot2011_COI	PS-C04	1	BB32975_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32975	Sample	BB32975	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C04	COI	45	BB32975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C04	CTTTAATTACAGCGTTTTTATTGTTGT	137.61315
BB32976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92643	WGS84	BB32976	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32976_Mgig_Bot2012_COI	PS-C05	1	BB32976_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32976	Sample	BB32976	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C05	COI	45	BB32976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C05	TCTTATTCGTTGGAGACTTTATAACCCT	137.62
BB32977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92558	WGS84	BB32977	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32977_Cmae_Bot2011_COI	PS-C06	1	BB32977_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32977	Sample	BB32977	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C06	COI	45	BB32977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C06	ATGAACAGTCTATCCTCCTTTAG	137.62398
BB32977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92558	WGS84	BB32977	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32977_Msal_Bot2012_COI	PS-C06	1	BB32977_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32977	Sample	BB32977	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C06	COI	45	BB32977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C06	GYTAGTTCCRATGATGTTAGCTG	137.62398
BB32979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92229	WGS84	BB32979	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32979_Aamu_Bax2006_COI	PS-C08	1	BB32979_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32979	Sample	BB32979	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C08	COI	45	BB32979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C08	GCACAACCGGGATCTTTACTTC	137.62767
BB32979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92229	WGS84	BB32979	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32979_Pcan_Bot2011_IGS	PS-C08	1	BB32979_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32979	Sample	BB32979	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C08	IGS	45	BB32979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C08	CGTAATCCTCAGTACTGGCTA	137.62767
BB32979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92229	WGS84	BB32979	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32979_Sspa_Oph2007_COI	PS-C08	1	BB32979_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32979	Sample	BB32979	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C08	COI	45	BB32979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C08	GAAGGCCTTCCGATGATC	137.62767
BB32979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92229	WGS84	BB32979	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32979_Upin_Bot2011_COI	PS-C08	1	BB32979_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32979	Sample	BB32979	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C08	COI	45	BB32979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C08	CTTTAATTACAGCGTTTTTATTGTTGT	137.62767
BB32980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92255	WGS84	BB32980	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32980_Cmae_Bot2011_COI	PS-C09	1	BB32980_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32980	Sample	BB32980	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C09	COI	45	BB32980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C09	ATGAACAGTCTATCCTCCTTTAG	137.63506
BB32980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92255	WGS84	BB32980	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32980_Msal_Bot2012_COI	PS-C09	1	BB32980_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32980	Sample	BB32980	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C09	COI	45	BB32980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C09	GYTAGTTCCRATGATGTTAGCTG	137.63506
BB32982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92987	WGS84	BB32982	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32982_Aamu_Bax2006_COI	PS-C11	1	BB32982_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32982	Sample	BB32982	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C11	COI	45	BB32982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C11	GCACAACCGGGATCTTTACTTC	137.61215
BB32982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92987	WGS84	BB32982	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32982_Pcan_Bot2011_IGS	PS-C11	1	BB32982_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32982	Sample	BB32982	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C11	IGS	45	BB32982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C11	CGTAATCCTCAGTACTGGCTA	137.61215
BB32982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92987	WGS84	BB32982	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32982_Sspa_Oph2007_COI	PS-C11	1	BB32982_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32982	Sample	BB32982	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C11	COI	45	BB32982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C11	GAAGGCCTTCCGATGATC	137.61215
BB32982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.92987	WGS84	BB32982	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32982_Upin_Bot2011_COI	PS-C11	1	BB32982_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32982	Sample	BB32982	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C11	COI	45	BB32982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C11	CTTTAATTACAGCGTTTTTATTGTTGT	137.61215
BB32983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93096	WGS84	BB32983	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32983_Mgig_Bot2012_COI	PS-C12	1	BB32983_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32983	Sample	BB32983	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C12	COI	45	BB32983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C12	TCTTATTCGTTGGAGACTTTATAACCCT	137.61016
BB32984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93119	WGS84	BB32984	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32984_Cmae_Bot2011_COI	PS-C13	1	BB32984_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32984	Sample	BB32984	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C13	COI	45	BB32984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C13	ATGAACAGTCTATCCTCCTTTAG	137.609
BB32984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06204	-33.93119	WGS84	BB32984	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32984_Msal_Bot2012_COI	PS-C13	1	BB32984_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32984	Sample	BB32984	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C13	COI	45	BB32984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C13	GYTAGTTCCRATGATGTTAGCTG	137.609
BB32986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92686	WGS84	BB32986	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32986_Aamu_Bax2006_COI	PS-C15	1	BB32986_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32986	Sample	BB32986	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C15	COI	45	BB32986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C15	GCACAACCGGGATCTTTACTTC	137.61371
BB32986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92686	WGS84	BB32986	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32986_Pcan_Bot2011_IGS	PS-C15	1	BB32986_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32986	Sample	BB32986	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C15	IGS	45	BB32986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C15	CGTAATCCTCAGTACTGGCTA	137.61371
BB32986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92686	WGS84	BB32986	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32986_Sspa_Oph2007_COI	PS-C15	1	BB32986_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32986	Sample	BB32986	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C15	COI	45	BB32986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C15	GAAGGCCTTCCGATGATC	137.61371
BB32986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92686	WGS84	BB32986	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32986_Upin_Bot2011_COI	PS-C15	1	BB32986_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32986	Sample	BB32986	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C15	COI	45	BB32986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C15	CTTTAATTACAGCGTTTTTATTGTTGT	137.61371
BB32987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92631	WGS84	BB32987	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32987_Mgig_Bot2012_COI	PS-C16	1	BB32987_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32987	Sample	BB32987	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C16	COI	45	BB32987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C16	TCTTATTCGTTGGAGACTTTATAACCCT	137.6116
BB32988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92372	WGS84	BB32988	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32988_Cmae_Bot2011_COI	PS-C17	1	BB32988_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32988	Sample	BB32988	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C17	COI	45	BB32988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C17	ATGAACAGTCTATCCTCCTTTAG	137.61164
BB32988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92372	WGS84	BB32988	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32988_Msal_Bot2012_COI	PS-C17	1	BB32988_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32988	Sample	BB32988	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C17	COI	45	BB32988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C17	GYTAGTTCCRATGATGTTAGCTG	137.61164
BB32990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92591	WGS84	BB32990	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32990_Mgig_Bot2012_COI	PS-C19	1	BB32990_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32990	Sample	BB32990	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C19	COI	45	BB32990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C19	TCTTATTCGTTGGAGACTTTATAACCCT	137.61858
BB32991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.9266	WGS84	BB32991	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32991_Cmae_Bot2011_COI	PS-C20	1	BB32991_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32991	Sample	BB32991	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C20	COI	45	BB32991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C20	ATGAACAGTCTATCCTCCTTTAG	137.62115
BB32991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.9266	WGS84	BB32991	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32991_Msal_Bot2012_COI	PS-C20	1	BB32991_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32991	Sample	BB32991	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C20	COI	45	BB32991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C20	GYTAGTTCCRATGATGTTAGCTG	137.62115
BB32993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92419	WGS84	BB32993	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32993_Aamu_Bax2006_COI	PS-C22	1	BB32993_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32993	Sample	BB32993	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C22	COI	45	BB32993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C22	GCACAACCGGGATCTTTACTTC	137.62419
BB32993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92419	WGS84	BB32993	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32993_Pcan_Bot2011_IGS	PS-C22	1	BB32993_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32993	Sample	BB32993	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C22	IGS	45	BB32993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C22	CGTAATCCTCAGTACTGGCTA	137.62419
BB32993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92419	WGS84	BB32993	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32993_Sspa_Oph2007_COI	PS-C22	1	BB32993_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32993	Sample	BB32993	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C22	COI	45	BB32993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C22	GAAGGCCTTCCGATGATC	137.62419
BB32993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92419	WGS84	BB32993	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32993_Upin_Bot2011_COI	PS-C22	1	BB32993_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32993	Sample	BB32993	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C22	COI	45	BB32993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C22	CTTTAATTACAGCGTTTTTATTGTTGT	137.62419
BB32994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92304	WGS84	BB32994	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32994_Mgig_Bot2012_COI	PS-C23	1	BB32994_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32994	Sample	BB32994	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C23	COI	45	BB32994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C23	TCTTATTCGTTGGAGACTTTATAACCCT	137.62427
BB32995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92047	WGS84	BB32995	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32995_Cmae_Bot2011_COI	PS-C24	1	BB32995_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32995	Sample	BB32995	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C24	COI	45	BB32995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C24	ATGAACAGTCTATCCTCCTTTAG	137.62665
BB32995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92047	WGS84	BB32995	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32995_Msal_Bot2012_COI	PS-C24	1	BB32995_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32995	Sample	BB32995	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C24	COI	45	BB32995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C24	GYTAGTTCCRATGATGTTAGCTG	137.62665
BB32997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92254	WGS84	BB32997	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB32997_Aamu_Bax2006_COI	PS-C26	1	BB32997_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32997	Sample	BB32997	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C26	COI	45	BB32997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C26	GCACAACCGGGATCTTTACTTC	137.62948
BB32997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92254	WGS84	BB32997	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB32997_Pcan_Bot2011_IGS	PS-C26	1	BB32997_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32997	Sample	BB32997	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C26	IGS	45	BB32997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C26	CGTAATCCTCAGTACTGGCTA	137.62948
BB32997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92254	WGS84	BB32997	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB32997_Sspa_Oph2007_COI	PS-C26	1	BB32997_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32997	Sample	BB32997	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C26	COI	45	BB32997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C26	GAAGGCCTTCCGATGATC	137.62948
BB32997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92254	WGS84	BB32997	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB32997_Upin_Bot2011_COI	PS-C26	1	BB32997_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32997	Sample	BB32997	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C26	COI	45	BB32997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C26	CTTTAATTACAGCGTTTTTATTGTTGT	137.62948
BB32998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92361	WGS84	BB32998	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB32998_Mgig_Bot2012_COI	PS-C27	1	BB32998_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32998	Sample	BB32998	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C27	COI	45	BB32998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C27	TCTTATTCGTTGGAGACTTTATAACCCT	137.63321
BB32999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92169	WGS84	BB32999	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB32999_Cmae_Bot2011_COI	PS-C28	1	BB32999_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32999	Sample	BB32999	targeted	Absent	Sample	Cmae_Bot2011_COI	PS-C28	COI	45	BB32999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C28	ATGAACAGTCTATCCTCCTTTAG	137.63669
BB32999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92169	WGS84	BB32999	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB32999_Msal_Bot2012_COI	PS-C28	1	BB32999_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB32999	Sample	BB32999	targeted	Absent	Sample	Msal_Bot2012_COI	PS-C28	COI	45	BB32999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C28	GYTAGTTCCRATGATGTTAGCTG	137.63669
BB33000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92206	WGS84	BB33000	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB33000_Aamu_Bax2006_COI	PS-C29	1	BB33000_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33000	Sample	BB33000	targeted	Absent	Sample	Aamu_Bax2006_COI	PS-C29	COI	45	BB33000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C29	GCACAACCGGGATCTTTACTTC	137.63842
BB33000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92206	WGS84	BB33000	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB33000_Pcan_Bot2011_IGS	PS-C29	1	BB33000_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33000	Sample	BB33000	targeted	Absent	Sample	Pcan_Bot2011_IGS	PS-C29	IGS	45	BB33000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C29	CGTAATCCTCAGTACTGGCTA	137.63842
BB33000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92206	WGS84	BB33000	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB33000_Sspa_Oph2007_COI	PS-C29	1	BB33000_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33000	Sample	BB33000	targeted	Absent	Sample	Sspa_Oph2007_COI	PS-C29	COI	45	BB33000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C29	GAAGGCCTTCCGATGATC	137.63842
BB33000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.92206	WGS84	BB33000	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB33000_Upin_Bot2011_COI	PS-C29	1	BB33000_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33000	Sample	BB33000	targeted	Absent	Sample	Upin_Bot2011_COI	PS-C29	COI	45	BB33000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C29	CTTTAATTACAGCGTTTTTATTGTTGT	137.63842
BB33001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06205	-33.91952	WGS84	BB33001	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2017) Applying molecular techniques to surveys for introduced marine pests at ports in Spencer Gulf, South Australia. Report for PIRSA Biosecurity SA. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000202-1. SARDI Research Report Series No. 947, Adelaide.	Spencer_Gulf_Port_surveys-Wallaroo	6/07/2016	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB33001_Mgig_Bot2012_COI	PS-C30	1	BB33001_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB33001	Sample	BB33001	targeted	Absent	Sample	Mgig_Bot2012_COI	PS-C30	COI	45	BB33001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PS-C30	TCTTATTCGTTGGAGACTTTATAACCCT	137.63993
BB46879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.81759	WGS84	BB46879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.60098	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46879_Sspa_Oph2007_COI	M38	1	BB46879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46879	Sample	BB46879	targeted	Present	Sample	Sspa_Oph2007_COI	M38	COI	45	BB46879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M38	GAAGGCCTTCCGATGATC	144.92113
BB46879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.81759	WGS84	BB46879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.235	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46879_Upin_Bot2011_COI	M38	1	BB46879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46879	Sample	BB46879	targeted	Present	Sample	Upin_Bot2011_COI	M38	COI	45	BB46879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M38	CTTTAATTACAGCGTTTTTATTGTTGT	144.92113
BB46882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.82777	WGS84	BB46882	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.086	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB46882_Aamu_Bax2006_COI	M41	1	BB46882_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46882	Sample	BB46882	targeted	Present	Sample	Aamu_Bax2006_COI	M41	COI	45	BB46882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M41	GCACAACCGGGATCTTTACTTC	144.90065
BB46882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.82777	WGS84	BB46882	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.553467	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46882_Sspa_Oph2007_COI	M41	1	BB46882_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46882	Sample	BB46882	targeted	Present	Sample	Sspa_Oph2007_COI	M41	COI	45	BB46882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M41	GAAGGCCTTCCGATGATC	144.90065
BB46882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.82777	WGS84	BB46882	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.63	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46882_Upin_Bot2011_COI	M41	1	BB46882_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46882	Sample	BB46882	targeted	Present	Sample	Upin_Bot2011_COI	M41	COI	45	BB46882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M41	CTTTAATTACAGCGTTTTTATTGTTGT	144.90065
BB46886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84803	WGS84	BB46886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.952637	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB46886_Sspa_Oph2007_COI	M45	1	BB46886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46886	Sample	BB46886	targeted	Present	Sample	Sspa_Oph2007_COI	M45	COI	45	BB46886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M45	GAAGGCCTTCCGATGATC	144.90501
BB46886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84803	WGS84	BB46886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.112	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46886_Upin_Bot2011_COI	M45	1	BB46886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46886	Sample	BB46886	targeted	Present	Sample	Upin_Bot2011_COI	M45	COI	45	BB46886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M45	CTTTAATTACAGCGTTTTTATTGTTGT	144.90501
BB46897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_06453	-37.84311	WGS84	BB46897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.504	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Giblot-Ducray D, Wiltshire KH (2017) Marine pests testing methods: contributing to establishment of an Australian Testing Centre for Marine Pests. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2017/000273-1. SARDI Research Report Series No. 963, Adelaide.	Marine_Pests_Testing-Melbourne_2	15/11/2016	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB46897_Upin_Bot2011_COI	M56	1	BB46897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB46897	Sample	BB46897	targeted	Present	Sample	Upin_Bot2011_COI	M56	COI	45	BB46897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	M56	CTTTAATTACAGCGTTTTTATTGTTGT	144.92538
BB57685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77745	WGS84	BB57685	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57685_Cjap_Sim2018_COI	S1-02	1	BB57685_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57685	Sample	BB57685	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-02	COI	45	BB57685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51629
BB57685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.77745	WGS84	BB57685	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.48	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57685_Mgig_Bot2012_COI	S1-02	1	BB57685_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57685	Sample	BB57685	targeted	Present	Sample	Mgig_Bot2012_COI	S1-02	COI	45	BB57685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.51629
BB57686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77915	WGS84	BB57686	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57686_Cmae_Bot2011_COI	S1-04	1	BB57686_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57686	Sample	BB57686	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-04	COI	45	BB57686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-04	ATGAACAGTCTATCCTCCTTTAG	138.51673
BB57688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8455	WGS84	BB57688	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57688_Aamu_Bax2006_COI	S1-09	1	BB57688_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57688	Sample	BB57688	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-09	COI	45	BB57688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-09	GCACAACCGGGATCTTTACTTC	138.49684
BB57688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8455	WGS84	BB57688	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57688_Pcan_Bot2011_IGS	S1-09	1	BB57688_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57688	Sample	BB57688	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-09	IGS	45	BB57688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-09	CGTAATCCTCAGTACTGGCTA	138.49684
BB57688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8455	WGS84	BB57688	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.365	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57688_Sspa_Oph2007_COI	S1-09	1	BB57688_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57688	Sample	BB57688	targeted	Present	Sample	Sspa_Oph2007_COI	S1-09	COI	45	BB57688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-09	GAAGGCCTTCCGATGATC	138.49684
BB57688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8455	WGS84	BB57688	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57688_Upin_Bot2011_COI	S1-09	1	BB57688_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57688	Sample	BB57688	targeted	Absent	Sample	Upin_Bot2011_COI	S1-09	COI	45	BB57688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-09	CTTTAATTACAGCGTTTTTATTGTTGT	138.49684
BB57689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.8371	WGS84	BB57689	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57689_Cjap_Sim2018_COI	S1-12	1	BB57689_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57689	Sample	BB57689	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-12	COI	45	BB57689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5104
BB57689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.8371	WGS84	BB57689	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57689_Mgig_Bot2012_COI	S1-12	1	BB57689_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57689	Sample	BB57689	targeted	Absent	Sample	Mgig_Bot2012_COI	S1-12	COI	45	BB57689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.5104
BB57691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80941	WGS84	BB57691	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57691_Aamu_Bax2006_COI	S1-17	1	BB57691_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57691	Sample	BB57691	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-17	COI	45	BB57691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-17	GCACAACCGGGATCTTTACTTC	138.52052
BB57691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80941	WGS84	BB57691	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57691_Pcan_Bot2011_IGS	S1-17	1	BB57691_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57691	Sample	BB57691	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-17	IGS	45	BB57691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-17	CGTAATCCTCAGTACTGGCTA	138.52052
BB57691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80941	WGS84	BB57691	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57691_Sspa_Oph2007_COI	S1-17	1	BB57691_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57691	Sample	BB57691	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-17	COI	45	BB57691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-17	GAAGGCCTTCCGATGATC	138.52052
BB57691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80941	WGS84	BB57691	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57691_Upin_Bot2011_COI	S1-17	1	BB57691_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57691	Sample	BB57691	targeted	Absent	Sample	Upin_Bot2011_COI	S1-17	COI	45	BB57691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.52052
BB57692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81094	WGS84	BB57692	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57692_Cjap_Sim2018_COI	S1-19	1	BB57692_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57692	Sample	BB57692	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-19	COI	45	BB57692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52211
BB57692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.81094	WGS84	BB57692	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57692_Mgig_Bot2012_COI	S1-19	1	BB57692_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57692	Sample	BB57692	targeted	Absent	Sample	Mgig_Bot2012_COI	S1-19	COI	45	BB57692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.52211
BB57693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.77643	WGS84	BB57693	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57693_Cmae_Bot2011_COI	S1-01	1	BB57693_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57693	Sample	BB57693	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-01	COI	45	BB57693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-01	ATGAACAGTCTATCCTCCTTTAG	138.51619
BB57695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78088	WGS84	BB57695	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57695_Aamu_Bax2006_COI	S1-05	1	BB57695_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57695	Sample	BB57695	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-05	COI	45	BB57695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-05	GCACAACCGGGATCTTTACTTC	138.51745
BB57695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78088	WGS84	BB57695	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57695_Pcan_Bot2011_IGS	S1-05	1	BB57695_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57695	Sample	BB57695	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-05	IGS	45	BB57695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-05	CGTAATCCTCAGTACTGGCTA	138.51745
BB57695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78088	WGS84	BB57695	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57695_Sspa_Oph2007_COI	S1-05	1	BB57695_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57695	Sample	BB57695	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-05	COI	45	BB57695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-05	GAAGGCCTTCCGATGATC	138.51745
BB57695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78088	WGS84	BB57695	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57695_Upin_Bot2011_COI	S1-05	1	BB57695_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57695	Sample	BB57695	targeted	Absent	Sample	Upin_Bot2011_COI	S1-05	COI	45	BB57695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.51745
BB57696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84503	WGS84	BB57696	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57696_Cjap_Sim2018_COI	S1-06	1	BB57696_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57696	Sample	BB57696	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-06	COI	45	BB57696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49721
BB57696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84503	WGS84	BB57696	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.788	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57696_Mgig_Bot2012_COI	S1-06	1	BB57696_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57696	Sample	BB57696	targeted	Present	Sample	Mgig_Bot2012_COI	S1-06	COI	45	BB57696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.49721
BB57697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84714	WGS84	BB57697	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57697_Cmae_Bot2011_COI	S1-08	1	BB57697_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57697	Sample	BB57697	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-08	COI	45	BB57697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-08	ATGAACAGTCTATCCTCCTTTAG	138.49732
BB57699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83652	WGS84	BB57699	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57699_Aamu_Bax2006_COI	S1-11	1	BB57699_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57699	Sample	BB57699	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-11	COI	45	BB57699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-11	GCACAACCGGGATCTTTACTTC	138.50885
BB57699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83652	WGS84	BB57699	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57699_Pcan_Bot2011_IGS	S1-11	1	BB57699_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57699	Sample	BB57699	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-11	IGS	45	BB57699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-11	CGTAATCCTCAGTACTGGCTA	138.50885
BB57699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83652	WGS84	BB57699	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57699_Sspa_Oph2007_COI	S1-11	1	BB57699_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57699	Sample	BB57699	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-11	COI	45	BB57699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-11	GAAGGCCTTCCGATGATC	138.50885
BB57699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83652	WGS84	BB57699	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57699_Upin_Bot2011_COI	S1-11	1	BB57699_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57699	Sample	BB57699	targeted	Absent	Sample	Upin_Bot2011_COI	S1-11	COI	45	BB57699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.50885
BB57700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83672	WGS84	BB57700	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57700_Cjap_Sim2018_COI	S1-13	1	BB57700_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57700	Sample	BB57700	targeted	Absent	Sample	Cjap_Sim2018_COI	S1-13	COI	45	BB57700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50894
BB57700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83672	WGS84	BB57700	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S01	28/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57700_Mgig_Bot2012_COI	S1-13	1	BB57700_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57700	Sample	BB57700	targeted	Absent	Sample	Mgig_Bot2012_COI	S1-13	COI	45	BB57700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.50894
BB57701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB57701	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57701_Cmae_Bot2011_COI	S1-15	1	BB57701_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57701	Sample	BB57701	targeted	Absent	Sample	Cmae_Bot2011_COI	S1-15	COI	45	BB57701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-15	ATGAACAGTCTATCCTCCTTTAG	138.50926
BB57703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81017	WGS84	BB57703	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57703_Aamu_Bax2006_COI	S1-18	1	BB57703_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57703	Sample	BB57703	targeted	Absent	Sample	Aamu_Bax2006_COI	S1-18	COI	45	BB57703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-18	GCACAACCGGGATCTTTACTTC	138.52224
BB57703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81017	WGS84	BB57703	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57703_Pcan_Bot2011_IGS	S1-18	1	BB57703_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57703	Sample	BB57703	targeted	Absent	Sample	Pcan_Bot2011_IGS	S1-18	IGS	45	BB57703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-18	CGTAATCCTCAGTACTGGCTA	138.52224
BB57703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81017	WGS84	BB57703	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57703_Sspa_Oph2007_COI	S1-18	1	BB57703_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57703	Sample	BB57703	targeted	Absent	Sample	Sspa_Oph2007_COI	S1-18	COI	45	BB57703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-18	GAAGGCCTTCCGATGATC	138.52224
BB57703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81017	WGS84	BB57703	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S01	28/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57703_Upin_Bot2011_COI	S1-18	1	BB57703_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57703	Sample	BB57703	targeted	Absent	Sample	Upin_Bot2011_COI	S1-18	COI	45	BB57703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S1-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.52224
BB57704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82663	WGS84	BB57704	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57704_Cjap_Sim2018_COI	T2-05	1	BB57704_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57704	Sample	BB57704	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-05	COI	45	BB57704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3027
BB57704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82663	WGS84	BB57704	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.872	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57704_Mgig_Bot2012_COI	T2-05	1	BB57704_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57704	Sample	BB57704	targeted	Present	Sample	Mgig_Bot2012_COI	T2-05	COI	45	BB57704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.3027
BB57705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87703	WGS84	BB57705	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.248	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57705_Cmae_Bot2011_COI	T2-13	1	BB57705_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57705	Sample	BB57705	targeted	Present	Sample	Cmae_Bot2011_COI	T2-13	COI	45	BB57705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-13	ATGAACAGTCTATCCTCCTTTAG	147.34127
BB57707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88341	WGS84	BB57707	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57707_Aamu_Bax2006_COI	T2-14	1	BB57707_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57707	Sample	BB57707	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-14	COI	45	BB57707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-14	GCACAACCGGGATCTTTACTTC	147.33708
BB57707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88341	WGS84	BB57707	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57707_Pcan_Bot2011_IGS	T2-14	1	BB57707_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57707	Sample	BB57707	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-14	IGS	45	BB57707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-14	CGTAATCCTCAGTACTGGCTA	147.33708
BB57707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88341	WGS84	BB57707	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57707_Sspa_Oph2007_COI	T2-14	1	BB57707_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57707	Sample	BB57707	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-14	COI	45	BB57707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-14	GAAGGCCTTCCGATGATC	147.33708
BB57707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88341	WGS84	BB57707	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57707_Upin_Bot2011_COI	T2-14	1	BB57707_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57707	Sample	BB57707	targeted	Absent	Sample	Upin_Bot2011_COI	T2-14	COI	45	BB57707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.33708
BB57708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9596	WGS84	BB57708	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57708_Cjap_Sim2018_COI	T2-07	1	BB57708_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57708	Sample	BB57708	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-07	COI	45	BB57708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52467
BB57708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.9596	WGS84	BB57708	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.552	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	23/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57708_Mgig_Bot2012_COI	T2-07	1	BB57708_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57708	Sample	BB57708	targeted	Present	Sample	Mgig_Bot2012_COI	T2-07	COI	45	BB57708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.52467
BB57709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96028	WGS84	BB57709	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.897	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57709_Cmae_Bot2011_COI	T3-03	1	BB57709_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57709	Sample	BB57709	targeted	Present	Sample	Cmae_Bot2011_COI	T3-03	COI	45	BB57709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-03	ATGAACAGTCTATCCTCCTTTAG	147.53093
BB57710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85216	WGS84	BB57710	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57710_Aamu_Bax2006_COI	T2-08	1	BB57710_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57710	Sample	BB57710	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-08	COI	45	BB57710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-08	GCACAACCGGGATCTTTACTTC	147.87352
BB57710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85216	WGS84	BB57710	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57710_Pcan_Bot2011_IGS	T2-08	1	BB57710_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57710	Sample	BB57710	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-08	IGS	45	BB57710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-08	CGTAATCCTCAGTACTGGCTA	147.87352
BB57710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85216	WGS84	BB57710	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57710_Sspa_Oph2007_COI	T2-08	1	BB57710_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57710	Sample	BB57710	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-08	COI	45	BB57710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-08	GAAGGCCTTCCGATGATC	147.87352
BB57710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85216	WGS84	BB57710	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.454	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57710_Upin_Bot2011_COI	T2-08	1	BB57710_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57710	Sample	BB57710	targeted	Present	Sample	Upin_Bot2011_COI	T2-08	COI	45	BB57710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.87352
BB57711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95936	WGS84	BB57711	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57711_Cjap_Sim2018_COI	T3-05	1	BB57711_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57711	Sample	BB57711	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-05	COI	45	BB57711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52541
BB57711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.95936	WGS84	BB57711	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.489	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	4/04/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57711_Mgig_Bot2012_COI	T3-05	1	BB57711_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57711	Sample	BB57711	targeted	Present	Sample	Mgig_Bot2012_COI	T3-05	COI	45	BB57711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.52541
BB57712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82837	WGS84	BB57712	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.624	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57712_Cmae_Bot2011_COI	T2-01	1	BB57712_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57712	Sample	BB57712	targeted	Present	Sample	Cmae_Bot2011_COI	T2-01	COI	45	BB57712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-01	ATGAACAGTCTATCCTCCTTTAG	147.30181
BB57714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87762	WGS84	BB57714	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57714_Aamu_Bax2006_COI	T2-15	1	BB57714_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57714	Sample	BB57714	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-15	COI	45	BB57714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-15	GCACAACCGGGATCTTTACTTC	147.34147
BB57714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87762	WGS84	BB57714	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57714_Pcan_Bot2011_IGS	T2-15	1	BB57714_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57714	Sample	BB57714	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-15	IGS	45	BB57714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-15	CGTAATCCTCAGTACTGGCTA	147.34147
BB57714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87762	WGS84	BB57714	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57714_Sspa_Oph2007_COI	T2-15	1	BB57714_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57714	Sample	BB57714	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-15	COI	45	BB57714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-15	GAAGGCCTTCCGATGATC	147.34147
BB57714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87762	WGS84	BB57714	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57714_Upin_Bot2011_COI	T2-15	1	BB57714_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57714	Sample	BB57714	targeted	Absent	Sample	Upin_Bot2011_COI	T2-15	COI	45	BB57714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.34147
BB57715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88566	WGS84	BB57715	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57715_Cjap_Sim2018_COI	T2-02	1	BB57715_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57715	Sample	BB57715	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-02	COI	45	BB57715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33452
BB57715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88566	WGS84	BB57715	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.373	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	21/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57715_Mgig_Bot2012_COI	T2-02	1	BB57715_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57715	Sample	BB57715	targeted	Present	Sample	Mgig_Bot2012_COI	T2-02	COI	45	BB57715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.33452
BB57716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88465	WGS84	BB57716	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	21/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57716_Cmae_Bot2011_COI	T2-10	1	BB57716_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57716	Sample	BB57716	targeted	Absent	Sample	Cmae_Bot2011_COI	T2-10	COI	45	BB57716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-10	ATGAACAGTCTATCCTCCTTTAG	147.33621
BB57718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9601	WGS84	BB57718	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57718_Aamu_Bax2006_COI	T2-03	1	BB57718_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57718	Sample	BB57718	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-03	COI	45	BB57718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-03	GCACAACCGGGATCTTTACTTC	147.53037
BB57718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9601	WGS84	BB57718	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57718_Pcan_Bot2011_IGS	T2-03	1	BB57718_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57718	Sample	BB57718	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-03	IGS	45	BB57718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-03	CGTAATCCTCAGTACTGGCTA	147.53037
BB57718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9601	WGS84	BB57718	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57718_Sspa_Oph2007_COI	T2-03	1	BB57718_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57718	Sample	BB57718	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-03	COI	45	BB57718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-03	GAAGGCCTTCCGATGATC	147.53037
BB57718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.9601	WGS84	BB57718	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57718_Upin_Bot2011_COI	T2-03	1	BB57718_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57718	Sample	BB57718	targeted	Absent	Sample	Upin_Bot2011_COI	T2-03	COI	45	BB57718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.53037
BB57719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96959	WGS84	BB57719	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	23/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57719_Cjap_Sim2018_COI	T2-11	1	BB57719_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57719	Sample	BB57719	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-11	COI	45	BB57719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51602
BB57719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96959	WGS84	BB57719	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.857	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	23/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57719_Mgig_Bot2012_COI	T2-11	1	BB57719_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57719	Sample	BB57719	targeted	Present	Sample	Mgig_Bot2012_COI	T2-11	COI	45	BB57719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.51602
BB57721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84817	WGS84	BB57721	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57721_Aamu_Bax2006_COI	T2-04	1	BB57721_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57721	Sample	BB57721	targeted	Absent	Sample	Aamu_Bax2006_COI	T2-04	COI	45	BB57721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-04	GCACAACCGGGATCTTTACTTC	147.8728
BB57721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84817	WGS84	BB57721	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57721_Pcan_Bot2011_IGS	T2-04	1	BB57721_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57721	Sample	BB57721	targeted	Absent	Sample	Pcan_Bot2011_IGS	T2-04	IGS	45	BB57721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-04	CGTAATCCTCAGTACTGGCTA	147.8728
BB57721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84817	WGS84	BB57721	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57721_Sspa_Oph2007_COI	T2-04	1	BB57721_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57721	Sample	BB57721	targeted	Absent	Sample	Sspa_Oph2007_COI	T2-04	COI	45	BB57721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-04	GAAGGCCTTCCGATGATC	147.8728
BB57721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84817	WGS84	BB57721	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57721_Upin_Bot2011_COI	T2-04	1	BB57721_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57721	Sample	BB57721	targeted	Absent	Sample	Upin_Bot2011_COI	T2-04	COI	45	BB57721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.8728
BB57722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85306	WGS84	BB57722	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T02	26/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57722_Cjap_Sim2018_COI	T2-12	1	BB57722_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57722	Sample	BB57722	targeted	Absent	Sample	Cjap_Sim2018_COI	T2-12	COI	45	BB57722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87211
BB57722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85306	WGS84	BB57722	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T02	26/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57722_Mgig_Bot2012_COI	T2-12	1	BB57722_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57722	Sample	BB57722	targeted	Absent	Sample	Mgig_Bot2012_COI	T2-12	COI	45	BB57722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T2-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.87211
BB57723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87738	WGS84	BB57723	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57723_Cmae_Bot2011_COI	T3-04	1	BB57723_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57723	Sample	BB57723	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-04	COI	45	BB57723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-04	ATGAACAGTCTATCCTCCTTTAG	147.34147
BB57725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83693	WGS84	BB57725	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57725_Aamu_Bax2006_COI	S2-06	1	BB57725_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57725	Sample	BB57725	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-06	COI	45	BB57725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-06	GCACAACCGGGATCTTTACTTC	138.50748
BB57725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83693	WGS84	BB57725	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57725_Pcan_Bot2011_IGS	S2-06	1	BB57725_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57725	Sample	BB57725	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-06	IGS	45	BB57725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-06	CGTAATCCTCAGTACTGGCTA	138.50748
BB57725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83693	WGS84	BB57725	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.304	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57725_Sspa_Oph2007_COI	S2-06	1	BB57725_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57725	Sample	BB57725	targeted	Present	Sample	Sspa_Oph2007_COI	S2-06	COI	45	BB57725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-06	GAAGGCCTTCCGATGATC	138.50748
BB57725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83693	WGS84	BB57725	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57725_Upin_Bot2011_COI	S2-06	1	BB57725_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57725	Sample	BB57725	targeted	Absent	Sample	Upin_Bot2011_COI	S2-06	COI	45	BB57725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.50748
BB57726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.7866	WGS84	BB57726	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57726_Cjap_Sim2018_COI	S2-12	1	BB57726_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57726	Sample	BB57726	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-12	COI	45	BB57726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51435
BB57726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.7866	WGS84	BB57726	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57726_Mgig_Bot2012_COI	S2-12	1	BB57726_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57726	Sample	BB57726	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-12	COI	45	BB57726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.51435
BB57727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83676	WGS84	BB57727	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57727_Cmae_Bot2011_COI	S2-09	1	BB57727_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57727	Sample	BB57727	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-09	COI	45	BB57727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-09	ATGAACAGTCTATCCTCCTTTAG	138.50937
BB57729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84482	WGS84	BB57729	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57729_Aamu_Bax2006_COI	S2-04	1	BB57729_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57729	Sample	BB57729	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-04	COI	45	BB57729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-04	GCACAACCGGGATCTTTACTTC	138.49676
BB57729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84482	WGS84	BB57729	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57729_Pcan_Bot2011_IGS	S2-04	1	BB57729_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57729	Sample	BB57729	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-04	IGS	45	BB57729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-04	CGTAATCCTCAGTACTGGCTA	138.49676
BB57729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84482	WGS84	BB57729	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.879	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57729_Sspa_Oph2007_COI	S2-04	1	BB57729_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57729	Sample	BB57729	targeted	Present	Sample	Sspa_Oph2007_COI	S2-04	COI	45	BB57729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-04	GAAGGCCTTCCGATGATC	138.49676
BB57729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84482	WGS84	BB57729	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57729_Upin_Bot2011_COI	S2-04	1	BB57729_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57729	Sample	BB57729	targeted	Absent	Sample	Upin_Bot2011_COI	S2-04	COI	45	BB57729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.49676
BB57730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84664	WGS84	BB57730	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57730_Cmae_Bot2011_COI	S2-02	1	BB57730_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57730	Sample	BB57730	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-02	COI	45	BB57730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-02	ATGAACAGTCTATCCTCCTTTAG	138.49722
BB57732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85213	WGS84	BB57732	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57732_Aamu_Bax2006_COI	T3-07	1	BB57732_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57732	Sample	BB57732	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-07	COI	45	BB57732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-07	GCACAACCGGGATCTTTACTTC	147.87312
BB57732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85213	WGS84	BB57732	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57732_Pcan_Bot2011_IGS	T3-07	1	BB57732_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57732	Sample	BB57732	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-07	IGS	45	BB57732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-07	CGTAATCCTCAGTACTGGCTA	147.87312
BB57732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85213	WGS84	BB57732	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57732_Sspa_Oph2007_COI	T3-07	1	BB57732_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57732	Sample	BB57732	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-07	COI	45	BB57732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-07	GAAGGCCTTCCGATGATC	147.87312
BB57732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85213	WGS84	BB57732	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57732_Upin_Bot2011_COI	T3-07	1	BB57732_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57732	Sample	BB57732	targeted	Absent	Sample	Upin_Bot2011_COI	T3-07	COI	45	BB57732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.87312
BB57733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82744	WGS84	BB57733	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57733_Cjap_Sim2018_COI	T3-08	1	BB57733_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57733	Sample	BB57733	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-08	COI	45	BB57733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30218
BB57733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.82744	WGS84	BB57733	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.307	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57733_Mgig_Bot2012_COI	T3-08	1	BB57733_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57733	Sample	BB57733	targeted	Present	Sample	Mgig_Bot2012_COI	T3-08	COI	45	BB57733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.30218
BB57734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85563	WGS84	BB57734	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57734_Cmae_Bot2011_COI	T3-12	1	BB57734_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57734	Sample	BB57734	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-12	COI	45	BB57734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-12	ATGAACAGTCTATCCTCCTTTAG	147.87212
BB57736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82869	WGS84	BB57736	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57736_Aamu_Bax2006_COI	T3-01	1	BB57736_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57736	Sample	BB57736	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-01	COI	45	BB57736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-01	GCACAACCGGGATCTTTACTTC	147.30215
BB57736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82869	WGS84	BB57736	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57736_Pcan_Bot2011_IGS	T3-01	1	BB57736_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57736	Sample	BB57736	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-01	IGS	45	BB57736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-01	CGTAATCCTCAGTACTGGCTA	147.30215
BB57736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82869	WGS84	BB57736	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57736_Sspa_Oph2007_COI	T3-01	1	BB57736_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57736	Sample	BB57736	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-01	COI	45	BB57736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-01	GAAGGCCTTCCGATGATC	147.30215
BB57736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82869	WGS84	BB57736	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57736_Upin_Bot2011_COI	T3-01	1	BB57736_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57736	Sample	BB57736	targeted	Absent	Sample	Upin_Bot2011_COI	T3-01	COI	45	BB57736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.30215
BB57737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88386	WGS84	BB57737	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57737_Cjap_Sim2018_COI	T3-11	1	BB57737_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57737	Sample	BB57737	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-11	COI	45	BB57737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33667
BB57737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.88386	WGS84	BB57737	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.262	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57737_Mgig_Bot2012_COI	T3-11	1	BB57737_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57737	Sample	BB57737	targeted	Present	Sample	Mgig_Bot2012_COI	T3-11	COI	45	BB57737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.33667
BB57738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88476	WGS84	BB57738	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57738_Cmae_Bot2011_COI	T3-17	1	BB57738_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57738	Sample	BB57738	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-17	COI	45	BB57738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-17	ATGAACAGTCTATCCTCCTTTAG	147.33551
BB57740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96226	WGS84	BB57740	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	4/04/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57740_Cjap_Sim2018_COI	T3-10	1	BB57740_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57740	Sample	BB57740	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-10	COI	45	BB57740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51947
BB57740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96226	WGS84	BB57740	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.581	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	4/04/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57740_Mgig_Bot2012_COI	T3-10	1	BB57740_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57740	Sample	BB57740	targeted	Present	Sample	Mgig_Bot2012_COI	T3-10	COI	45	BB57740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.51947
BB57741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88326	WGS84	BB57741	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB57741_Cmae_Bot2011_COI	T3-16	1	BB57741_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57741	Sample	BB57741	targeted	Absent	Sample	Cmae_Bot2011_COI	T3-16	COI	45	BB57741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-16	ATGAACAGTCTATCCTCCTTTAG	147.33803
BB57743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88241	WGS84	BB57743	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB57743_Aamu_Bax2006_COI	T3-15	1	BB57743_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57743	Sample	BB57743	targeted	Absent	Sample	Aamu_Bax2006_COI	T3-15	COI	45	BB57743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-15	GCACAACCGGGATCTTTACTTC	147.33604
BB57743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88241	WGS84	BB57743	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB57743_Pcan_Bot2011_IGS	T3-15	1	BB57743_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57743	Sample	BB57743	targeted	Absent	Sample	Pcan_Bot2011_IGS	T3-15	IGS	45	BB57743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-15	CGTAATCCTCAGTACTGGCTA	147.33604
BB57743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88241	WGS84	BB57743	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB57743_Sspa_Oph2007_COI	T3-15	1	BB57743_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57743	Sample	BB57743	targeted	Absent	Sample	Sspa_Oph2007_COI	T3-15	COI	45	BB57743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-15	GAAGGCCTTCCGATGATC	147.33604
BB57743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88241	WGS84	BB57743	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB57743_Upin_Bot2011_COI	T3-15	1	BB57743_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57743	Sample	BB57743	targeted	Absent	Sample	Upin_Bot2011_COI	T3-15	COI	45	BB57743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.33604
BB57744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87892	WGS84	BB57744	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T03	30/03/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB57744_Cjap_Sim2018_COI	T3-14	1	BB57744_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57744	Sample	BB57744	targeted	Absent	Sample	Cjap_Sim2018_COI	T3-14	COI	45	BB57744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34139
BB57744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87892	WGS84	BB57744	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T03	30/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB57744_Mgig_Bot2012_COI	T3-14	1	BB57744_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB57744	Sample	BB57744	targeted	Absent	Sample	Mgig_Bot2012_COI	T3-14	COI	45	BB57744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T3-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.34139
BB73901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82045	WGS84	BB73901	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73901_Cmae_Bot2011_COI	MA51	1	BB73901_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73901	Sample	BB73901	targeted	Absent	Sample	Cmae_Bot2011_COI	MA51	COI	45	BB73901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA51	ATGAACAGTCTATCCTCCTTTAG	144.93041
BB73901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82045	WGS84	BB73901	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73901_Msal_Bot2012_COI	MA51	1	BB73901_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73901	Sample	BB73901	targeted	Absent	Sample	Msal_Bot2012_COI	MA51	COI	45	BB73901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA51	GYTAGTTCCRATGATGTTAGCTG	144.93041
BB73903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81657	WGS84	BB73903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	20.894	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73903_Aamu_Bax2006_COI	MA48	1	BB73903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73903	Sample	BB73903	targeted	Present	Sample	Aamu_Bax2006_COI	MA48	COI	45	BB73903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA48	GCACAACCGGGATCTTTACTTC	144.92576
BB73903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81657	WGS84	BB73903	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73903_Pcan_Bot2011_IGS	MA48	1	BB73903_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73903	Sample	BB73903	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA48	IGS	45	BB73903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA48	CGTAATCCTCAGTACTGGCTA	144.92576
BB73903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81657	WGS84	BB73903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73903_Sspa_Oph2007_COI	MA48	1	BB73903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73903	Sample	BB73903	targeted	Absent	Sample	Sspa_Oph2007_COI	MA48	COI	45	BB73903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA48	GAAGGCCTTCCGATGATC	144.92576
BB73903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81657	WGS84	BB73903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73903_Upin_Bot2011_COI	MA48	1	BB73903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73903	Sample	BB73903	targeted	Absent	Sample	Upin_Bot2011_COI	MA48	COI	45	BB73903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA48	CTTTAATTACAGCGTTTTTATTGTTGT	144.92576
BB73904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82918	WGS84	BB73904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73904_Mgig_Bot2012_COI	MA47	1	BB73904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73904	Sample	BB73904	targeted	Absent	Sample	Mgig_Bot2012_COI	MA47	COI	45	BB73904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA47	TCTTATTCGTTGGAGACTTTATAACCCT	144.90038
BB73905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.35748	WGS84	BB73905	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73905_Cmae_Bot2011_COI	BA20	1	BB73905_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73905	Sample	BB73905	targeted	Absent	Sample	Cmae_Bot2011_COI	BA20	COI	45	BB73905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA20	ATGAACAGTCTATCCTCCTTTAG	153.18045
BB73905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.35748	WGS84	BB73905	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73905_Msal_Bot2012_COI	BA20	1	BB73905_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73905	Sample	BB73905	targeted	Absent	Sample	Msal_Bot2012_COI	BA20	COI	45	BB73905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA20	GYTAGTTCCRATGATGTTAGCTG	153.18045
BB73907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81685	WGS84	BB73907	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.156	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73907_Aamu_Bax2006_COI	MA7	1	BB73907_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73907	Sample	BB73907	targeted	Present	Sample	Aamu_Bax2006_COI	MA7	COI	45	BB73907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA7	GCACAACCGGGATCTTTACTTC	144.92339
BB73907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81685	WGS84	BB73907	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73907_Pcan_Bot2011_IGS	MA7	1	BB73907_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73907	Sample	BB73907	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA7	IGS	45	BB73907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA7	CGTAATCCTCAGTACTGGCTA	144.92339
BB73907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81685	WGS84	BB73907	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73907_Sspa_Oph2007_COI	MA7	1	BB73907_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73907	Sample	BB73907	targeted	Absent	Sample	Sspa_Oph2007_COI	MA7	COI	45	BB73907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA7	GAAGGCCTTCCGATGATC	144.92339
BB73907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81685	WGS84	BB73907	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73907_Upin_Bot2011_COI	MA7	1	BB73907_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73907	Sample	BB73907	targeted	Absent	Sample	Upin_Bot2011_COI	MA7	COI	45	BB73907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA7	CTTTAATTACAGCGTTTTTATTGTTGT	144.92339
BB73908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81914	WGS84	BB73908	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73908_Mgig_Bot2012_COI	MA6	1	BB73908_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73908	Sample	BB73908	targeted	Absent	Sample	Mgig_Bot2012_COI	MA6	COI	45	BB73908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA6	TCTTATTCGTTGGAGACTTTATAACCCT	144.92582
BB73909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.4463	WGS84	BB73909	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73909_Cmae_Bot2011_COI	BA6	1	BB73909_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73909	Sample	BB73909	targeted	Absent	Sample	Cmae_Bot2011_COI	BA6	COI	45	BB73909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA6	ATGAACAGTCTATCCTCCTTTAG	153.07695
BB73909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.4463	WGS84	BB73909	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73909_Msal_Bot2012_COI	BA6	1	BB73909_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73909	Sample	BB73909	targeted	Absent	Sample	Msal_Bot2012_COI	BA6	COI	45	BB73909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA6	GYTAGTTCCRATGATGTTAGCTG	153.07695
BB73910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44688	WGS84	BB73910	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73910_Aamu_Bax2006_COI	BA11	1	BB73910_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73910	Sample	BB73910	targeted	Absent	Sample	Aamu_Bax2006_COI	BA11	COI	45	BB73910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA11	GCACAACCGGGATCTTTACTTC	153.07904
BB73910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44688	WGS84	BB73910	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73910_Pcan_Bot2011_IGS	BA11	1	BB73910_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73910	Sample	BB73910	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA11	IGS	45	BB73910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA11	CGTAATCCTCAGTACTGGCTA	153.07904
BB73910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44688	WGS84	BB73910	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73910_Sspa_Oph2007_COI	BA11	1	BB73910_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73910	Sample	BB73910	targeted	Absent	Sample	Sspa_Oph2007_COI	BA11	COI	45	BB73910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA11	GAAGGCCTTCCGATGATC	153.07904
BB73910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44688	WGS84	BB73910	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73910_Upin_Bot2011_COI	BA11	1	BB73910_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73910	Sample	BB73910	targeted	Absent	Sample	Upin_Bot2011_COI	BA11	COI	45	BB73910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA11	CTTTAATTACAGCGTTTTTATTGTTGT	153.07904
BB73911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB73911	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73911_Mgig_Bot2012_COI	MA2	1	BB73911_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73911	Sample	BB73911	targeted	Absent	Sample	Mgig_Bot2012_COI	MA2	COI	45	BB73911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA2	TCTTATTCGTTGGAGACTTTATAACCCT	144.93107
BB73912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81865	WGS84	BB73912	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73912_Cmae_Bot2011_COI	MA1	1	BB73912_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73912	Sample	BB73912	targeted	Absent	Sample	Cmae_Bot2011_COI	MA1	COI	45	BB73912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA1	ATGAACAGTCTATCCTCCTTTAG	144.9245
BB73912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81865	WGS84	BB73912	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73912_Msal_Bot2012_COI	MA1	1	BB73912_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73912	Sample	BB73912	targeted	Absent	Sample	Msal_Bot2012_COI	MA1	COI	45	BB73912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA1	GYTAGTTCCRATGATGTTAGCTG	144.9245
BB73914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44441	WGS84	BB73914	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73914_Aamu_Bax2006_COI	BA12	1	BB73914_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73914	Sample	BB73914	targeted	Absent	Sample	Aamu_Bax2006_COI	BA12	COI	45	BB73914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA12	GCACAACCGGGATCTTTACTTC	153.07844
BB73914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44441	WGS84	BB73914	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73914_Pcan_Bot2011_IGS	BA12	1	BB73914_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73914	Sample	BB73914	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA12	IGS	45	BB73914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA12	CGTAATCCTCAGTACTGGCTA	153.07844
BB73914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44441	WGS84	BB73914	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73914_Sspa_Oph2007_COI	BA12	1	BB73914_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73914	Sample	BB73914	targeted	Absent	Sample	Sspa_Oph2007_COI	BA12	COI	45	BB73914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA12	GAAGGCCTTCCGATGATC	153.07844
BB73914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44441	WGS84	BB73914	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73914_Upin_Bot2011_COI	BA12	1	BB73914_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73914	Sample	BB73914	targeted	Absent	Sample	Upin_Bot2011_COI	BA12	COI	45	BB73914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA12	CTTTAATTACAGCGTTTTTATTGTTGT	153.07844
BB73915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82059	WGS84	BB73915	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73915_Mgig_Bot2012_COI	GA100	1	BB73915_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73915	Sample	BB73915	targeted	Absent	Sample	Mgig_Bot2012_COI	GA100	COI	45	BB73915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA100	TCTTATTCGTTGGAGACTTTATAACCCT	144.90597
BB73916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82708	WGS84	BB73916	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73916_Cmae_Bot2011_COI	GA95	1	BB73916_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73916	Sample	BB73916	targeted	Absent	Sample	Cmae_Bot2011_COI	GA95	COI	45	BB73916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA95	ATGAACAGTCTATCCTCCTTTAG	144.89976
BB73916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82708	WGS84	BB73916	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73916_Msal_Bot2012_COI	GA95	1	BB73916_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73916	Sample	BB73916	targeted	Absent	Sample	Msal_Bot2012_COI	GA95	COI	45	BB73916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA95	GYTAGTTCCRATGATGTTAGCTG	144.89976
BB73918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81632	WGS84	BB73918	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	19.682	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73918_Aamu_Bax2006_COI	GA85	1	BB73918_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73918	Sample	BB73918	targeted	Present	Sample	Aamu_Bax2006_COI	GA85	COI	45	BB73918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA85	GCACAACCGGGATCTTTACTTC	144.91413
BB73918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81632	WGS84	BB73918	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73918_Pcan_Bot2011_IGS	GA85	1	BB73918_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73918	Sample	BB73918	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA85	IGS	45	BB73918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA85	CGTAATCCTCAGTACTGGCTA	144.91413
BB73918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81632	WGS84	BB73918	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73918_Sspa_Oph2007_COI	GA85	1	BB73918_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73918	Sample	BB73918	targeted	Absent	Sample	Sspa_Oph2007_COI	GA85	COI	45	BB73918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA85	GAAGGCCTTCCGATGATC	144.91413
BB73918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81632	WGS84	BB73918	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73918_Upin_Bot2011_COI	GA85	1	BB73918_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73918	Sample	BB73918	targeted	Absent	Sample	Upin_Bot2011_COI	GA85	COI	45	BB73918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA85	CTTTAATTACAGCGTTTTTATTGTTGT	144.91413
BB73919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81129	WGS84	BB73919	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73919_Mgig_Bot2012_COI	GA80	1	BB73919_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73919	Sample	BB73919	targeted	Absent	Sample	Mgig_Bot2012_COI	GA80	COI	45	BB73919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA80	TCTTATTCGTTGGAGACTTTATAACCCT	144.91354
BB73921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82095	WGS84	BB73921	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.089	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73921_Aamu_Bax2006_COI	GA70	1	BB73921_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73921	Sample	BB73921	targeted	Present	Sample	Aamu_Bax2006_COI	GA70	COI	45	BB73921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA70	GCACAACCGGGATCTTTACTTC	144.90757
BB73921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82095	WGS84	BB73921	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73921_Pcan_Bot2011_IGS	GA70	1	BB73921_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73921	Sample	BB73921	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA70	IGS	45	BB73921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA70	CGTAATCCTCAGTACTGGCTA	144.90757
BB73921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82095	WGS84	BB73921	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73921_Sspa_Oph2007_COI	GA70	1	BB73921_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73921	Sample	BB73921	targeted	Absent	Sample	Sspa_Oph2007_COI	GA70	COI	45	BB73921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA70	GAAGGCCTTCCGATGATC	144.90757
BB73921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82095	WGS84	BB73921	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73921_Upin_Bot2011_COI	GA70	1	BB73921_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73921	Sample	BB73921	targeted	Absent	Sample	Upin_Bot2011_COI	GA70	COI	45	BB73921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA70	CTTTAATTACAGCGTTTTTATTGTTGT	144.90757
BB73922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82287	WGS84	BB73922	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73922_Mgig_Bot2012_COI	GA65	1	BB73922_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73922	Sample	BB73922	targeted	Absent	Sample	Mgig_Bot2012_COI	GA65	COI	45	BB73922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA65	TCTTATTCGTTGGAGACTTTATAACCCT	144.90529
BB73923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44494	WGS84	BB73923	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73923_Cmae_Bot2011_COI	BA13	1	BB73923_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73923	Sample	BB73923	targeted	Absent	Sample	Cmae_Bot2011_COI	BA13	COI	45	BB73923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA13	ATGAACAGTCTATCCTCCTTTAG	153.08106
BB73923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44494	WGS84	BB73923	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73923_Msal_Bot2012_COI	BA13	1	BB73923_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73923	Sample	BB73923	targeted	Absent	Sample	Msal_Bot2012_COI	BA13	COI	45	BB73923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA13	GYTAGTTCCRATGATGTTAGCTG	153.08106
BB73925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81443	WGS84	BB73925	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	18.243	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73925_Aamu_Bax2006_COI	GA45	1	BB73925_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73925	Sample	BB73925	targeted	Present	Sample	Aamu_Bax2006_COI	GA45	COI	45	BB73925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA45	GCACAACCGGGATCTTTACTTC	144.91251
BB73925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81443	WGS84	BB73925	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73925_Pcan_Bot2011_IGS	GA45	1	BB73925_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73925	Sample	BB73925	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA45	IGS	45	BB73925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA45	CGTAATCCTCAGTACTGGCTA	144.91251
BB73925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81443	WGS84	BB73925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.186	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73925_Sspa_Oph2007_COI	GA45	1	BB73925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73925	Sample	BB73925	targeted	Present	Sample	Sspa_Oph2007_COI	GA45	COI	45	BB73925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA45	GAAGGCCTTCCGATGATC	144.91251
BB73925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81443	WGS84	BB73925	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73925_Upin_Bot2011_COI	GA45	1	BB73925_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73925	Sample	BB73925	targeted	Absent	Sample	Upin_Bot2011_COI	GA45	COI	45	BB73925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA45	CTTTAATTACAGCGTTTTTATTGTTGT	144.91251
BB73926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44611	WGS84	BB73926	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73926_Mgig_Bot2012_COI	BA14	1	BB73926_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73926	Sample	BB73926	targeted	Absent	Sample	Mgig_Bot2012_COI	BA14	COI	45	BB73926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA14	TCTTATTCGTTGGAGACTTTATAACCCT	153.08337
BB73927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81859	WGS84	BB73927	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73927_Cmae_Bot2011_COI	GA35	1	BB73927_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73927	Sample	BB73927	targeted	Absent	Sample	Cmae_Bot2011_COI	GA35	COI	45	BB73927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA35	ATGAACAGTCTATCCTCCTTTAG	144.90601
BB73927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81859	WGS84	BB73927	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73927_Msal_Bot2012_COI	GA35	1	BB73927_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73927	Sample	BB73927	targeted	Absent	Sample	Msal_Bot2012_COI	GA35	COI	45	BB73927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA35	GYTAGTTCCRATGATGTTAGCTG	144.90601
BB73929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81279	WGS84	BB73929	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	20.816	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73929_Aamu_Bax2006_COI	GA25	1	BB73929_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73929	Sample	BB73929	targeted	Present	Sample	Aamu_Bax2006_COI	GA25	COI	45	BB73929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA25	GCACAACCGGGATCTTTACTTC	144.91442
BB73929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81279	WGS84	BB73929	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73929_Pcan_Bot2011_IGS	GA25	1	BB73929_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73929	Sample	BB73929	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA25	IGS	45	BB73929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA25	CGTAATCCTCAGTACTGGCTA	144.91442
BB73929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81279	WGS84	BB73929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.673	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73929_Sspa_Oph2007_COI	GA25	1	BB73929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73929	Sample	BB73929	targeted	Present	Sample	Sspa_Oph2007_COI	GA25	COI	45	BB73929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA25	GAAGGCCTTCCGATGATC	144.91442
BB73929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81279	WGS84	BB73929	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73929_Upin_Bot2011_COI	GA25	1	BB73929_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73929	Sample	BB73929	targeted	Absent	Sample	Upin_Bot2011_COI	GA25	COI	45	BB73929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA25	CTTTAATTACAGCGTTTTTATTGTTGT	144.91442
BB73930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37364	WGS84	BB73930	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73930_Cmae_Bot2011_COI	BA30	1	BB73930_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73930	Sample	BB73930	targeted	Absent	Sample	Cmae_Bot2011_COI	BA30	COI	45	BB73930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA30	ATGAACAGTCTATCCTCCTTTAG	153.16922
BB73930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37364	WGS84	BB73930	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73930_Msal_Bot2012_COI	BA30	1	BB73930_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73930	Sample	BB73930	targeted	Absent	Sample	Msal_Bot2012_COI	BA30	COI	45	BB73930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA30	GYTAGTTCCRATGATGTTAGCTG	153.16922
BB73932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82371	WGS84	BB73932	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.918	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73932_Aamu_Bax2006_COI	GA10	1	BB73932_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73932	Sample	BB73932	targeted	Present	Sample	Aamu_Bax2006_COI	GA10	COI	45	BB73932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA10	GCACAACCGGGATCTTTACTTC	144.90329
BB73932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82371	WGS84	BB73932	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73932_Pcan_Bot2011_IGS	GA10	1	BB73932_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73932	Sample	BB73932	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA10	IGS	45	BB73932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA10	CGTAATCCTCAGTACTGGCTA	144.90329
BB73932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82371	WGS84	BB73932	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73932_Sspa_Oph2007_COI	GA10	1	BB73932_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73932	Sample	BB73932	targeted	Absent	Sample	Sspa_Oph2007_COI	GA10	COI	45	BB73932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA10	GAAGGCCTTCCGATGATC	144.90329
BB73932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82371	WGS84	BB73932	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73932_Upin_Bot2011_COI	GA10	1	BB73932_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73932	Sample	BB73932	targeted	Absent	Sample	Upin_Bot2011_COI	GA10	COI	45	BB73932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA10	CTTTAATTACAGCGTTTTTATTGTTGT	144.90329
BB73933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43303	WGS84	BB73933	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73933_Mgig_Bot2012_COI	BA18	1	BB73933_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73933	Sample	BB73933	targeted	Absent	Sample	Mgig_Bot2012_COI	BA18	COI	45	BB73933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA18	TCTTATTCGTTGGAGACTTTATAACCCT	153.11629
BB73934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44723	WGS84	BB73934	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73934_Cmae_Bot2011_COI	BA15	1	BB73934_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73934	Sample	BB73934	targeted	Absent	Sample	Cmae_Bot2011_COI	BA15	COI	45	BB73934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA15	ATGAACAGTCTATCCTCCTTTAG	153.09789
BB73934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44723	WGS84	BB73934	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73934_Msal_Bot2012_COI	BA15	1	BB73934_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73934	Sample	BB73934	targeted	Absent	Sample	Msal_Bot2012_COI	BA15	COI	45	BB73934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA15	GYTAGTTCCRATGATGTTAGCTG	153.09789
BB73936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3596	WGS84	BB73936	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73936_Aamu_Bax2006_COI	BA22	1	BB73936_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73936	Sample	BB73936	targeted	Absent	Sample	Aamu_Bax2006_COI	BA22	COI	45	BB73936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA22	GCACAACCGGGATCTTTACTTC	153.17773
BB73936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3596	WGS84	BB73936	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73936_Pcan_Bot2011_IGS	BA22	1	BB73936_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73936	Sample	BB73936	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA22	IGS	45	BB73936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA22	CGTAATCCTCAGTACTGGCTA	153.17773
BB73936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3596	WGS84	BB73936	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73936_Sspa_Oph2007_COI	BA22	1	BB73936_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73936	Sample	BB73936	targeted	Absent	Sample	Sspa_Oph2007_COI	BA22	COI	45	BB73936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA22	GAAGGCCTTCCGATGATC	153.17773
BB73936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3596	WGS84	BB73936	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73936_Upin_Bot2011_COI	BA22	1	BB73936_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73936	Sample	BB73936	targeted	Absent	Sample	Upin_Bot2011_COI	BA22	COI	45	BB73936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA22	CTTTAATTACAGCGTTTTTATTGTTGT	153.17773
BB73937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86045	WGS84	BB73937	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73937_Mgig_Bot2012_COI	MA87	1	BB73937_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73937	Sample	BB73937	targeted	Absent	Sample	Mgig_Bot2012_COI	MA87	COI	45	BB73937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA87	TCTTATTCGTTGGAGACTTTATAACCCT	144.91439
BB73938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36254	WGS84	BB73938	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73938_Cmae_Bot2011_COI	BA24	1	BB73938_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73938	Sample	BB73938	targeted	Absent	Sample	Cmae_Bot2011_COI	BA24	COI	45	BB73938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA24	ATGAACAGTCTATCCTCCTTTAG	153.17555
BB73938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.36254	WGS84	BB73938	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73938_Msal_Bot2012_COI	BA24	1	BB73938_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73938	Sample	BB73938	targeted	Absent	Sample	Msal_Bot2012_COI	BA24	COI	45	BB73938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA24	GYTAGTTCCRATGATGTTAGCTG	153.17555
BB73940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.3702	WGS84	BB73940	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73940_Mgig_Bot2012_COI	BA28	1	BB73940_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73940	Sample	BB73940	targeted	Absent	Sample	Mgig_Bot2012_COI	BA28	COI	45	BB73940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA28	TCTTATTCGTTGGAGACTTTATAACCCT	153.1708
BB73941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86508	WGS84	BB73941	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73941_Cmae_Bot2011_COI	MA81	1	BB73941_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73941	Sample	BB73941	targeted	Absent	Sample	Cmae_Bot2011_COI	MA81	COI	45	BB73941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA81	ATGAACAGTCTATCCTCCTTTAG	144.91763
BB73941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86508	WGS84	BB73941	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73941_Msal_Bot2012_COI	MA81	1	BB73941_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73941	Sample	BB73941	targeted	Absent	Sample	Msal_Bot2012_COI	MA81	COI	45	BB73941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA81	GYTAGTTCCRATGATGTTAGCTG	144.91763
BB73943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8606	WGS84	BB73943	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.71	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73943_Aamu_Bax2006_COI	MA78	1	BB73943_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73943	Sample	BB73943	targeted	Present	Sample	Aamu_Bax2006_COI	MA78	COI	45	BB73943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA78	GCACAACCGGGATCTTTACTTC	144.91438
BB73943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8606	WGS84	BB73943	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73943_Pcan_Bot2011_IGS	MA78	1	BB73943_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73943	Sample	BB73943	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA78	IGS	45	BB73943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA78	CGTAATCCTCAGTACTGGCTA	144.91438
BB73943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8606	WGS84	BB73943	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.64	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73943_Sspa_Oph2007_COI	MA78	1	BB73943_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73943	Sample	BB73943	targeted	Present	Sample	Sspa_Oph2007_COI	MA78	COI	45	BB73943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA78	GAAGGCCTTCCGATGATC	144.91438
BB73943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8606	WGS84	BB73943	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.145	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73943_Upin_Bot2011_COI	MA78	1	BB73943_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73943	Sample	BB73943	targeted	Present	Sample	Upin_Bot2011_COI	MA78	COI	45	BB73943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA78	CTTTAATTACAGCGTTTTTATTGTTGT	144.91438
BB73944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86423	WGS84	BB73944	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73944_Mgig_Bot2012_COI	MA76	1	BB73944_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73944	Sample	BB73944	targeted	Absent	Sample	Mgig_Bot2012_COI	MA76	COI	45	BB73944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA76	TCTTATTCGTTGGAGACTTTATAACCCT	144.91825
BB73945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43165	WGS84	BB73945	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73945_Cmae_Bot2011_COI	BA65	1	BB73945_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73945	Sample	BB73945	targeted	Absent	Sample	Cmae_Bot2011_COI	BA65	COI	45	BB73945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA65	ATGAACAGTCTATCCTCCTTTAG	153.12161
BB73945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.43165	WGS84	BB73945	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73945_Msal_Bot2012_COI	BA65	1	BB73945_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73945	Sample	BB73945	targeted	Absent	Sample	Msal_Bot2012_COI	BA65	COI	45	BB73945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA65	GYTAGTTCCRATGATGTTAGCTG	153.12161
BB73947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37905	WGS84	BB73947	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73947_Aamu_Bax2006_COI	BA34	1	BB73947_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73947	Sample	BB73947	targeted	Absent	Sample	Aamu_Bax2006_COI	BA34	COI	45	BB73947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA34	GCACAACCGGGATCTTTACTTC	153.16432
BB73947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37905	WGS84	BB73947	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73947_Pcan_Bot2011_IGS	BA34	1	BB73947_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73947	Sample	BB73947	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA34	IGS	45	BB73947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA34	CGTAATCCTCAGTACTGGCTA	153.16432
BB73947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37905	WGS84	BB73947	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73947_Sspa_Oph2007_COI	BA34	1	BB73947_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73947	Sample	BB73947	targeted	Absent	Sample	Sspa_Oph2007_COI	BA34	COI	45	BB73947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA34	GAAGGCCTTCCGATGATC	153.16432
BB73947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.37905	WGS84	BB73947	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73947_Upin_Bot2011_COI	BA34	1	BB73947_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73947	Sample	BB73947	targeted	Absent	Sample	Upin_Bot2011_COI	BA34	COI	45	BB73947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA34	CTTTAATTACAGCGTTTTTATTGTTGT	153.16432
BB73948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38147	WGS84	BB73948	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73948_Mgig_Bot2012_COI	BA36	1	BB73948_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73948	Sample	BB73948	targeted	Absent	Sample	Mgig_Bot2012_COI	BA36	COI	45	BB73948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA36	TCTTATTCGTTGGAGACTTTATAACCCT	153.16215
BB73949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8579	WGS84	BB73949	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73949_Cmae_Bot2011_COI	MA43	1	BB73949_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73949	Sample	BB73949	targeted	Absent	Sample	Cmae_Bot2011_COI	MA43	COI	45	BB73949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA43	ATGAACAGTCTATCCTCCTTTAG	144.91104
BB73949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8579	WGS84	BB73949	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73949_Msal_Bot2012_COI	MA43	1	BB73949_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73949	Sample	BB73949	targeted	Absent	Sample	Msal_Bot2012_COI	MA43	COI	45	BB73949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA43	GYTAGTTCCRATGATGTTAGCTG	144.91104
BB73950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38365	WGS84	BB73950	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73950_Aamu_Bax2006_COI	BA38	1	BB73950_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73950	Sample	BB73950	targeted	Absent	Sample	Aamu_Bax2006_COI	BA38	COI	45	BB73950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA38	GCACAACCGGGATCTTTACTTC	153.16045
BB73950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38365	WGS84	BB73950	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73950_Pcan_Bot2011_IGS	BA38	1	BB73950_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73950	Sample	BB73950	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA38	IGS	45	BB73950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA38	CGTAATCCTCAGTACTGGCTA	153.16045
BB73950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38365	WGS84	BB73950	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73950_Sspa_Oph2007_COI	BA38	1	BB73950_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73950	Sample	BB73950	targeted	Absent	Sample	Sspa_Oph2007_COI	BA38	COI	45	BB73950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA38	GAAGGCCTTCCGATGATC	153.16045
BB73950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38365	WGS84	BB73950	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73950_Upin_Bot2011_COI	BA38	1	BB73950_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73950	Sample	BB73950	targeted	Absent	Sample	Upin_Bot2011_COI	BA38	COI	45	BB73950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA38	CTTTAATTACAGCGTTTTTATTGTTGT	153.16045
BB73951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85613	WGS84	BB73951	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73951_Mgig_Bot2012_COI	MA19	1	BB73951_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73951	Sample	BB73951	targeted	Absent	Sample	Mgig_Bot2012_COI	MA19	COI	45	BB73951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA19	TCTTATTCGTTGGAGACTTTATAACCCT	144.90637
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73952_Cmae_Bot2011_COI	MA18	1	BB73952_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Absent	Sample	Cmae_Bot2011_COI	MA18	COI	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	ATGAACAGTCTATCCTCCTTTAG	144.90781
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73952_Hsan_Wil2021_COI	MA18	1	BB73952_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Absent	Sample	Hsan_Wil2021_COI	MA18	COI	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	CCTGGGCCGGTATAGTAGGT	144.90781
BB73952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85639	WGS84	BB73952	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73952_Msal_Bot2012_COI	MA18	1	BB73952_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73952	Sample	BB73952	targeted	Absent	Sample	Msal_Bot2012_COI	MA18	COI	45	BB73952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA18	GYTAGTTCCRATGATGTTAGCTG	144.90781
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.671	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73954_Aamu_Bax2006_COI	MA14	1	BB73954_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Present	Sample	Aamu_Bax2006_COI	MA14	COI	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	GCACAACCGGGATCTTTACTTC	144.91044
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73954_Pcan_Bot2011_IGS	MA14	1	BB73954_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA14	IGS	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	CGTAATCCTCAGTACTGGCTA	144.91044
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.76	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73954_Sspa_Oph2007_COI	MA14	1	BB73954_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Present	Sample	Sspa_Oph2007_COI	MA14	COI	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	GAAGGCCTTCCGATGATC	144.91044
BB73954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86018	WGS84	BB73954	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.984	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73954_Upin_Bot2011_COI	MA14	1	BB73954_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73954	Sample	BB73954	targeted	Present	Sample	Upin_Bot2011_COI	MA14	COI	45	BB73954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA14	CTTTAATTACAGCGTTTTTATTGTTGT	144.91044
BB73955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86288	WGS84	BB73955	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73955_Mgig_Bot2012_COI	MA13	1	BB73955_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73955	Sample	BB73955	targeted	Absent	Sample	Mgig_Bot2012_COI	MA13	COI	45	BB73955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA13	TCTTATTCGTTGGAGACTTTATAACCCT	144.9161
BB73956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38681	WGS84	BB73956	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73956_Cmae_Bot2011_COI	BA40	1	BB73956_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73956	Sample	BB73956	targeted	Absent	Sample	Cmae_Bot2011_COI	BA40	COI	45	BB73956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA40	ATGAACAGTCTATCCTCCTTTAG	153.15913
BB73956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38681	WGS84	BB73956	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73956_Msal_Bot2012_COI	BA40	1	BB73956_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73956	Sample	BB73956	targeted	Absent	Sample	Msal_Bot2012_COI	BA40	COI	45	BB73956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA40	GYTAGTTCCRATGATGTTAGCTG	153.15913
BB73958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38913	WGS84	BB73958	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73958_Aamu_Bax2006_COI	BA42	1	BB73958_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73958	Sample	BB73958	targeted	Absent	Sample	Aamu_Bax2006_COI	BA42	COI	45	BB73958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA42	GCACAACCGGGATCTTTACTTC	153.15793
BB73958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38913	WGS84	BB73958	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73958_Pcan_Bot2011_IGS	BA42	1	BB73958_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73958	Sample	BB73958	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA42	IGS	45	BB73958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA42	CGTAATCCTCAGTACTGGCTA	153.15793
BB73958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38913	WGS84	BB73958	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73958_Sspa_Oph2007_COI	BA42	1	BB73958_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73958	Sample	BB73958	targeted	Absent	Sample	Sspa_Oph2007_COI	BA42	COI	45	BB73958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA42	GAAGGCCTTCCGATGATC	153.15793
BB73958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38913	WGS84	BB73958	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73958_Upin_Bot2011_COI	BA42	1	BB73958_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73958	Sample	BB73958	targeted	Absent	Sample	Upin_Bot2011_COI	BA42	COI	45	BB73958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA42	CTTTAATTACAGCGTTTTTATTGTTGT	153.15793
BB73959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.85832	WGS84	BB73959	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73959_Mgig_Bot2012_COI	MA8	1	BB73959_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73959	Sample	BB73959	targeted	Absent	Sample	Mgig_Bot2012_COI	MA8	COI	45	BB73959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA8	TCTTATTCGTTGGAGACTTTATAACCCT	144.9099
BB73961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44508	WGS84	BB73961	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73961_Aamu_Bax2006_COI	BA5	1	BB73961_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73961	Sample	BB73961	targeted	Absent	Sample	Aamu_Bax2006_COI	BA5	COI	45	BB73961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA5	GCACAACCGGGATCTTTACTTC	153.07678
BB73961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44508	WGS84	BB73961	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73961_Pcan_Bot2011_IGS	BA5	1	BB73961_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73961	Sample	BB73961	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA5	IGS	45	BB73961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA5	CGTAATCCTCAGTACTGGCTA	153.07678
BB73961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44508	WGS84	BB73961	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73961_Sspa_Oph2007_COI	BA5	1	BB73961_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73961	Sample	BB73961	targeted	Absent	Sample	Sspa_Oph2007_COI	BA5	COI	45	BB73961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA5	GAAGGCCTTCCGATGATC	153.07678
BB73961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44508	WGS84	BB73961	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73961_Upin_Bot2011_COI	BA5	1	BB73961_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73961	Sample	BB73961	targeted	Absent	Sample	Upin_Bot2011_COI	BA5	COI	45	BB73961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA5	CTTTAATTACAGCGTTTTTATTGTTGT	153.07678
BB73962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84926	WGS84	BB73962	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73962_Mgig_Bot2012_COI	MA33	1	BB73962_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73962	Sample	BB73962	targeted	Absent	Sample	Mgig_Bot2012_COI	MA33	COI	45	BB73962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA33	TCTTATTCGTTGGAGACTTTATAACCCT	144.91212
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73963_Cmae_Bot2011_COI	MA32	1	BB73963_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Absent	Sample	Cmae_Bot2011_COI	MA32	COI	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	ATGAACAGTCTATCCTCCTTTAG	144.90916
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73963_Hsan_Wil2021_COI	MA32	1	BB73963_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Absent	Sample	Hsan_Wil2021_COI	MA32	COI	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	CCTGGGCCGGTATAGTAGGT	144.90916
BB73963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84553	WGS84	BB73963	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73963_Msal_Bot2012_COI	MA32	1	BB73963_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73963	Sample	BB73963	targeted	Absent	Sample	Msal_Bot2012_COI	MA32	COI	45	BB73963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA32	GYTAGTTCCRATGATGTTAGCTG	144.90916
BB73965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40104	WGS84	BB73965	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73965_Aamu_Bax2006_COI	BA46	1	BB73965_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73965	Sample	BB73965	targeted	Absent	Sample	Aamu_Bax2006_COI	BA46	COI	45	BB73965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA46	GCACAACCGGGATCTTTACTTC	153.16167
BB73965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40104	WGS84	BB73965	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73965_Pcan_Bot2011_IGS	BA46	1	BB73965_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73965	Sample	BB73965	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA46	IGS	45	BB73965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA46	CGTAATCCTCAGTACTGGCTA	153.16167
BB73965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40104	WGS84	BB73965	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73965_Sspa_Oph2007_COI	BA46	1	BB73965_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73965	Sample	BB73965	targeted	Absent	Sample	Sspa_Oph2007_COI	BA46	COI	45	BB73965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA46	GAAGGCCTTCCGATGATC	153.16167
BB73965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40104	WGS84	BB73965	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73965_Upin_Bot2011_COI	BA46	1	BB73965_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73965	Sample	BB73965	targeted	Absent	Sample	Upin_Bot2011_COI	BA46	COI	45	BB73965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA46	CTTTAATTACAGCGTTTTTATTGTTGT	153.16167
BB73966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84444	WGS84	BB73966	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73966_Mgig_Bot2012_COI	MA27	1	BB73966_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73966	Sample	BB73966	targeted	Absent	Sample	Mgig_Bot2012_COI	MA27	COI	45	BB73966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA27	TCTTATTCGTTGGAGACTTTATAACCCT	144.90642
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73967_Cmae_Bot2011_COI	MA26	1	BB73967_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Absent	Sample	Cmae_Bot2011_COI	MA26	COI	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	ATGAACAGTCTATCCTCCTTTAG	144.90739
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73967_Hsan_Wil2021_COI	MA26	1	BB73967_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Absent	Sample	Hsan_Wil2021_COI	MA26	COI	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	CCTGGGCCGGTATAGTAGGT	144.90739
BB73967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8451	WGS84	BB73967	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73967_Msal_Bot2012_COI	MA26	1	BB73967_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73967	Sample	BB73967	targeted	Absent	Sample	Msal_Bot2012_COI	MA26	COI	45	BB73967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA26	GYTAGTTCCRATGATGTTAGCTG	144.90739
BB73969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40088	WGS84	BB73969	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73969_Aamu_Bax2006_COI	BA48	1	BB73969_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73969	Sample	BB73969	targeted	Absent	Sample	Aamu_Bax2006_COI	BA48	COI	45	BB73969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA48	GCACAACCGGGATCTTTACTTC	153.15618
BB73969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40088	WGS84	BB73969	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73969_Pcan_Bot2011_IGS	BA48	1	BB73969_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73969	Sample	BB73969	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA48	IGS	45	BB73969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA48	CGTAATCCTCAGTACTGGCTA	153.15618
BB73969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40088	WGS84	BB73969	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73969_Sspa_Oph2007_COI	BA48	1	BB73969_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73969	Sample	BB73969	targeted	Absent	Sample	Sspa_Oph2007_COI	BA48	COI	45	BB73969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA48	GAAGGCCTTCCGATGATC	153.15618
BB73969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40088	WGS84	BB73969	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73969_Upin_Bot2011_COI	BA48	1	BB73969_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73969	Sample	BB73969	targeted	Absent	Sample	Upin_Bot2011_COI	BA48	COI	45	BB73969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA48	CTTTAATTACAGCGTTTTTATTGTTGT	153.15618
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73970_Cmae_Bot2011_COI	MA22	1	BB73970_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Absent	Sample	Cmae_Bot2011_COI	MA22	COI	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	ATGAACAGTCTATCCTCCTTTAG	144.90906
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73970_Hsan_Wil2021_COI	MA22	1	BB73970_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Absent	Sample	Hsan_Wil2021_COI	MA22	COI	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	CCTGGGCCGGTATAGTAGGT	144.90906
BB73970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84892	WGS84	BB73970	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73970_Msal_Bot2012_COI	MA22	1	BB73970_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73970	Sample	BB73970	targeted	Absent	Sample	Msal_Bot2012_COI	MA22	COI	45	BB73970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA22	GYTAGTTCCRATGATGTTAGCTG	144.90906
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.468	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73972_Aamu_Bax2006_COI	MA126	1	BB73972_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Present	Sample	Aamu_Bax2006_COI	MA126	COI	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	GCACAACCGGGATCTTTACTTC	144.93196
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73972_Pcan_Bot2011_IGS	MA126	1	BB73972_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA126	IGS	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	CGTAATCCTCAGTACTGGCTA	144.93196
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73972_Sspa_Oph2007_COI	MA126	1	BB73972_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Absent	Sample	Sspa_Oph2007_COI	MA126	COI	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	GAAGGCCTTCCGATGATC	144.93196
BB73972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84668	WGS84	BB73972	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.155	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73972_Upin_Bot2011_COI	MA126	1	BB73972_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73972	Sample	BB73972	targeted	Present	Sample	Upin_Bot2011_COI	MA126	COI	45	BB73972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA126	CTTTAATTACAGCGTTTTTATTGTTGT	144.93196
BB73973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40277	WGS84	BB73973	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73973_Mgig_Bot2012_COI	BA49	1	BB73973_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73973	Sample	BB73973	targeted	Absent	Sample	Mgig_Bot2012_COI	BA49	COI	45	BB73973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA49	TCTTATTCGTTGGAGACTTTATAACCCT	153.15406
BB73974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40781	WGS84	BB73974	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73974_Cmae_Bot2011_COI	BA52	1	BB73974_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73974	Sample	BB73974	targeted	Absent	Sample	Cmae_Bot2011_COI	BA52	COI	45	BB73974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA52	ATGAACAGTCTATCCTCCTTTAG	153.1502
BB73974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.40781	WGS84	BB73974	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73974_Msal_Bot2012_COI	BA52	1	BB73974_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73974	Sample	BB73974	targeted	Absent	Sample	Msal_Bot2012_COI	BA52	COI	45	BB73974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA52	GYTAGTTCCRATGATGTTAGCTG	153.1502
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.429	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73976_Aamu_Bax2006_COI	MA117	1	BB73976_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Present	Sample	Aamu_Bax2006_COI	MA117	COI	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	GCACAACCGGGATCTTTACTTC	144.92668
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73976_Pcan_Bot2011_IGS	MA117	1	BB73976_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA117	IGS	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	CGTAATCCTCAGTACTGGCTA	144.92668
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.606	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73976_Sspa_Oph2007_COI	MA117	1	BB73976_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Present	Sample	Sspa_Oph2007_COI	MA117	COI	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	GAAGGCCTTCCGATGATC	144.92668
BB73976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84297	WGS84	BB73976	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.429	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73976_Upin_Bot2011_COI	MA117	1	BB73976_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73976	Sample	BB73976	targeted	Present	Sample	Upin_Bot2011_COI	MA117	COI	45	BB73976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA117	CTTTAATTACAGCGTTTTTATTGTTGT	144.92668
BB73977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84828	WGS84	BB73977	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73977_Mgig_Bot2012_COI	MA116	1	BB73977_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73977	Sample	BB73977	targeted	Absent	Sample	Mgig_Bot2012_COI	MA116	COI	45	BB73977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA116	TCTTATTCGTTGGAGACTTTATAACCCT	144.93082
BB73978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.41523	WGS84	BB73978	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73978_Cmae_Bot2011_COI	BA53	1	BB73978_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73978	Sample	BB73978	targeted	Absent	Sample	Cmae_Bot2011_COI	BA53	COI	45	BB73978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA53	ATGAACAGTCTATCCTCCTTTAG	153.14303
BB73978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.41523	WGS84	BB73978	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73978_Msal_Bot2012_COI	BA53	1	BB73978_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73978	Sample	BB73978	targeted	Absent	Sample	Msal_Bot2012_COI	BA53	COI	45	BB73978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA53	GYTAGTTCCRATGATGTTAGCTG	153.14303
BB73980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42093	WGS84	BB73980	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73980_Mgig_Bot2012_COI	BA54	1	BB73980_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73980	Sample	BB73980	targeted	Absent	Sample	Mgig_Bot2012_COI	BA54	COI	45	BB73980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA54	TCTTATTCGTTGGAGACTTTATAACCCT	153.13708
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73981_Cmae_Bot2011_COI	MA108	1	BB73981_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Absent	Sample	Cmae_Bot2011_COI	MA108	COI	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	ATGAACAGTCTATCCTCCTTTAG	144.92753
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73981_Hsan_Wil2021_COI	MA108	1	BB73981_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Absent	Sample	Hsan_Wil2021_COI	MA108	COI	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	CCTGGGCCGGTATAGTAGGT	144.92753
BB73981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.84679	WGS84	BB73981	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73981_Msal_Bot2012_COI	MA108	1	BB73981_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73981	Sample	BB73981	targeted	Absent	Sample	Msal_Bot2012_COI	MA108	COI	45	BB73981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA108	GYTAGTTCCRATGATGTTAGCTG	144.92753
BB73983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80862	WGS84	BB73983	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73983_Aamu_Bax2006_COI	GA12	1	BB73983_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73983	Sample	BB73983	targeted	Absent	Sample	Aamu_Bax2006_COI	GA12	COI	45	BB73983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA12	GCACAACCGGGATCTTTACTTC	151.21623
BB73983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80862	WGS84	BB73983	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73983_Pcan_Bot2011_IGS	GA12	1	BB73983_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73983	Sample	BB73983	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA12	IGS	45	BB73983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA12	CGTAATCCTCAGTACTGGCTA	151.21623
BB73983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80862	WGS84	BB73983	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73983_Sspa_Oph2007_COI	GA12	1	BB73983_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73983	Sample	BB73983	targeted	Absent	Sample	Sspa_Oph2007_COI	GA12	COI	45	BB73983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA12	GAAGGCCTTCCGATGATC	151.21623
BB73983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80862	WGS84	BB73983	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73983_Upin_Bot2011_COI	GA12	1	BB73983_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73983	Sample	BB73983	targeted	Absent	Sample	Upin_Bot2011_COI	GA12	COI	45	BB73983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA12	CTTTAATTACAGCGTTTTTATTGTTGT	151.21623
BB73984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80612	WGS84	BB73984	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73984_Mgig_Bot2012_COI	GA11	1	BB73984_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73984	Sample	BB73984	targeted	Absent	Sample	Mgig_Bot2012_COI	GA11	COI	45	BB73984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA11	TCTTATTCGTTGGAGACTTTATAACCCT	151.21916
BB73985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80663	WGS84	BB73985	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73985_Cmae_Bot2011_COI	GA9	1	BB73985_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73985	Sample	BB73985	targeted	Absent	Sample	Cmae_Bot2011_COI	GA9	COI	45	BB73985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA9	ATGAACAGTCTATCCTCCTTTAG	151.21743
BB73985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80663	WGS84	BB73985	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73985_Msal_Bot2012_COI	GA9	1	BB73985_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73985	Sample	BB73985	targeted	Absent	Sample	Msal_Bot2012_COI	GA9	COI	45	BB73985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA9	GYTAGTTCCRATGATGTTAGCTG	151.21743
BB73987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80807	WGS84	BB73987	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73987_Aamu_Bax2006_COI	GA7	1	BB73987_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73987	Sample	BB73987	targeted	Absent	Sample	Aamu_Bax2006_COI	GA7	COI	45	BB73987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA7	GCACAACCGGGATCTTTACTTC	151.22001
BB73987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80807	WGS84	BB73987	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73987_Pcan_Bot2011_IGS	GA7	1	BB73987_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73987	Sample	BB73987	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA7	IGS	45	BB73987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA7	CGTAATCCTCAGTACTGGCTA	151.22001
BB73987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80807	WGS84	BB73987	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73987_Sspa_Oph2007_COI	GA7	1	BB73987_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73987	Sample	BB73987	targeted	Absent	Sample	Sspa_Oph2007_COI	GA7	COI	45	BB73987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA7	GAAGGCCTTCCGATGATC	151.22001
BB73987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80807	WGS84	BB73987	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73987_Upin_Bot2011_COI	GA7	1	BB73987_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73987	Sample	BB73987	targeted	Absent	Sample	Upin_Bot2011_COI	GA7	COI	45	BB73987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA7	CTTTAATTACAGCGTTTTTATTGTTGT	151.22001
BB73988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42525	WGS84	BB73988	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73988_Mgig_Bot2012_COI	BA57	1	BB73988_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73988	Sample	BB73988	targeted	Absent	Sample	Mgig_Bot2012_COI	BA57	COI	45	BB73988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA57	TCTTATTCGTTGGAGACTTTATAACCCT	153.12768
BB73989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80729	WGS84	BB73989	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73989_Cmae_Bot2011_COI	GA4	1	BB73989_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73989	Sample	BB73989	targeted	Absent	Sample	Cmae_Bot2011_COI	GA4	COI	45	BB73989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA4	ATGAACAGTCTATCCTCCTTTAG	151.21688
BB73989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80729	WGS84	BB73989	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73989_Msal_Bot2012_COI	GA4	1	BB73989_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73989	Sample	BB73989	targeted	Absent	Sample	Msal_Bot2012_COI	GA4	COI	45	BB73989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA4	GYTAGTTCCRATGATGTTAGCTG	151.21688
BB73990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42968	WGS84	BB73990	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73990_Aamu_Bax2006_COI	BA61	1	BB73990_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73990	Sample	BB73990	targeted	Absent	Sample	Aamu_Bax2006_COI	BA61	COI	45	BB73990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA61	GCACAACCGGGATCTTTACTTC	153.12444
BB73990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42968	WGS84	BB73990	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73990_Pcan_Bot2011_IGS	BA61	1	BB73990_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73990	Sample	BB73990	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA61	IGS	45	BB73990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA61	CGTAATCCTCAGTACTGGCTA	153.12444
BB73990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42968	WGS84	BB73990	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73990_Sspa_Oph2007_COI	BA61	1	BB73990_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73990	Sample	BB73990	targeted	Absent	Sample	Sspa_Oph2007_COI	BA61	COI	45	BB73990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA61	GAAGGCCTTCCGATGATC	153.12444
BB73990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42968	WGS84	BB73990	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73990_Upin_Bot2011_COI	BA61	1	BB73990_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73990	Sample	BB73990	targeted	Absent	Sample	Upin_Bot2011_COI	BA61	COI	45	BB73990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA61	CTTTAATTACAGCGTTTTTATTGTTGT	153.12444
BB73991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.80628	WGS84	BB73991	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73991_Mgig_Bot2012_COI	GA2	1	BB73991_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73991	Sample	BB73991	targeted	Absent	Sample	Mgig_Bot2012_COI	GA2	COI	45	BB73991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA2	TCTTATTCGTTGGAGACTTTATAACCCT	151.21494
BB73992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42892	WGS84	BB73992	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73992_Cmae_Bot2011_COI	BA64	1	BB73992_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73992	Sample	BB73992	targeted	Absent	Sample	Cmae_Bot2011_COI	BA64	COI	45	BB73992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA64	ATGAACAGTCTATCCTCCTTTAG	153.12154
BB73992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.42892	WGS84	BB73992	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	16/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73992_Msal_Bot2012_COI	BA64	1	BB73992_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73992	Sample	BB73992	targeted	Absent	Sample	Msal_Bot2012_COI	BA64	COI	45	BB73992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA64	GYTAGTTCCRATGATGTTAGCTG	153.12154
BB73994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85266	WGS84	BB73994	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73994_Aamu_Bax2006_COI	MA30	1	BB73994_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73994	Sample	BB73994	targeted	Absent	Sample	Aamu_Bax2006_COI	MA30	COI	45	BB73994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA30	GCACAACCGGGATCTTTACTTC	151.31613
BB73994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85266	WGS84	BB73994	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73994_Pcan_Bot2011_IGS	MA30	1	BB73994_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73994	Sample	BB73994	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA30	IGS	45	BB73994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA30	CGTAATCCTCAGTACTGGCTA	151.31613
BB73994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85266	WGS84	BB73994	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73994_Sspa_Oph2007_COI	MA30	1	BB73994_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73994	Sample	BB73994	targeted	Absent	Sample	Sspa_Oph2007_COI	MA30	COI	45	BB73994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA30	GAAGGCCTTCCGATGATC	151.31613
BB73994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85266	WGS84	BB73994	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73994_Upin_Bot2011_COI	MA30	1	BB73994_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73994	Sample	BB73994	targeted	Absent	Sample	Upin_Bot2011_COI	MA30	COI	45	BB73994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA30	CTTTAATTACAGCGTTTTTATTGTTGT	151.31613
BB73995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85533	WGS84	BB73995	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73995_Mgig_Bot2012_COI	MA25	1	BB73995_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73995	Sample	BB73995	targeted	Absent	Sample	Mgig_Bot2012_COI	MA25	COI	45	BB73995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA25	TCTTATTCGTTGGAGACTTTATAACCCT	151.32178
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.164	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB73996_Cmae_Bot2011_COI	MA97	1	BB73996_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Present	Sample	Cmae_Bot2011_COI	MA97	COI	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	ATGAACAGTCTATCCTCCTTTAG	144.93099
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB73996_Hsan_Wil2021_COI	MA97	1	BB73996_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Absent	Sample	Hsan_Wil2021_COI	MA97	COI	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	CCTGGGCCGGTATAGTAGGT	144.93099
BB73996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81821	WGS84	BB73996	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB73996_Msal_Bot2012_COI	MA97	1	BB73996_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73996	Sample	BB73996	targeted	Absent	Sample	Msal_Bot2012_COI	MA97	COI	45	BB73996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA97	GYTAGTTCCRATGATGTTAGCTG	144.93099
BB73998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38217	WGS84	BB73998	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB73998_Aamu_Bax2006_COI	BA37	1	BB73998_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73998	Sample	BB73998	targeted	Absent	Sample	Aamu_Bax2006_COI	BA37	COI	45	BB73998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA37	GCACAACCGGGATCTTTACTTC	153.16148
BB73998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38217	WGS84	BB73998	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB73998_Pcan_Bot2011_IGS	BA37	1	BB73998_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73998	Sample	BB73998	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA37	IGS	45	BB73998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA37	CGTAATCCTCAGTACTGGCTA	153.16148
BB73998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38217	WGS84	BB73998	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB73998_Sspa_Oph2007_COI	BA37	1	BB73998_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73998	Sample	BB73998	targeted	Absent	Sample	Sspa_Oph2007_COI	BA37	COI	45	BB73998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA37	GAAGGCCTTCCGATGATC	153.16148
BB73998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.38217	WGS84	BB73998	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	15/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB73998_Upin_Bot2011_COI	BA37	1	BB73998_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73998	Sample	BB73998	targeted	Absent	Sample	Upin_Bot2011_COI	BA37	COI	45	BB73998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA37	CTTTAATTACAGCGTTTTTATTGTTGT	153.16148
BB73999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.83984	WGS84	BB73999	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB73999_Mgig_Bot2012_COI	MA39	1	BB73999_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB73999	Sample	BB73999	targeted	Absent	Sample	Mgig_Bot2012_COI	MA39	COI	45	BB73999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA39	TCTTATTCGTTGGAGACTTTATAACCCT	144.90391
BB74001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88481	WGS84	BB74001	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.28	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74001_Aamu_Bax2006_COI	HA2	1	BB74001_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74001	Sample	BB74001	targeted	Present	Sample	Aamu_Bax2006_COI	HA2	COI	45	BB74001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA2	GCACAACCGGGATCTTTACTTC	147.3442
BB74001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88481	WGS84	BB74001	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74001_Pcan_Bot2011_IGS	HA2	1	BB74001_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74001	Sample	BB74001	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA2	IGS	45	BB74001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA2	CGTAATCCTCAGTACTGGCTA	147.3442
BB74001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88481	WGS84	BB74001	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74001_Sspa_Oph2007_COI	HA2	1	BB74001_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74001	Sample	BB74001	targeted	Absent	Sample	Sspa_Oph2007_COI	HA2	COI	45	BB74001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA2	GAAGGCCTTCCGATGATC	147.3442
BB74001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88481	WGS84	BB74001	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74001_Upin_Bot2011_COI	HA2	1	BB74001_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74001	Sample	BB74001	targeted	Absent	Sample	Upin_Bot2011_COI	HA2	COI	45	BB74001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA2	CTTTAATTACAGCGTTTTTATTGTTGT	147.3442
BB74002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.85391	WGS84	BB74002	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74002_Mgig_Bot2012_COI	GA27	1	BB74002_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74002	Sample	BB74002	targeted	Absent	Sample	Mgig_Bot2012_COI	GA27	COI	45	BB74002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA27	TCTTATTCGTTGGAGACTTTATAACCCT	151.31835
BB74003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8401	WGS84	BB74003	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74003_Cmae_Bot2011_COI	MA41	1	BB74003_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74003	Sample	BB74003	targeted	Absent	Sample	Cmae_Bot2011_COI	MA41	COI	45	BB74003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA41	ATGAACAGTCTATCCTCCTTTAG	144.90565
BB74003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8401	WGS84	BB74003	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74003_Msal_Bot2012_COI	MA41	1	BB74003_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74003	Sample	BB74003	targeted	Absent	Sample	Msal_Bot2012_COI	MA41	COI	45	BB74003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA41	GYTAGTTCCRATGATGTTAGCTG	144.90565
BB74005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86492	WGS84	BB74005	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.464	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74005_Aamu_Bax2006_COI	MA77	1	BB74005_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74005	Sample	BB74005	targeted	Present	Sample	Aamu_Bax2006_COI	MA77	COI	45	BB74005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA77	GCACAACCGGGATCTTTACTTC	144.91599
BB74005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86492	WGS84	BB74005	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74005_Pcan_Bot2011_IGS	MA77	1	BB74005_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74005	Sample	BB74005	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA77	IGS	45	BB74005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA77	CGTAATCCTCAGTACTGGCTA	144.91599
BB74005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86492	WGS84	BB74005	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.034	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74005_Sspa_Oph2007_COI	MA77	1	BB74005_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74005	Sample	BB74005	targeted	Present	Sample	Sspa_Oph2007_COI	MA77	COI	45	BB74005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA77	GAAGGCCTTCCGATGATC	144.91599
BB74005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.86492	WGS84	BB74005	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.354	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74005_Upin_Bot2011_COI	MA77	1	BB74005_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74005	Sample	BB74005	targeted	Present	Sample	Upin_Bot2011_COI	MA77	COI	45	BB74005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA77	CTTTAATTACAGCGTTTTTATTGTTGT	144.91599
BB74006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8879	WGS84	BB74006	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74006_Mgig_Bot2012_COI	HA4	1	BB74006_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74006	Sample	BB74006	targeted	Absent	Sample	Mgig_Bot2012_COI	HA4	COI	45	BB74006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA4	TCTTATTCGTTGGAGACTTTATAACCCT	147.3418
BB74007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78588	WGS84	BB74007	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74007_Cmae_Bot2011_COI	MA60	1	BB74007_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74007	Sample	BB74007	targeted	Absent	Sample	Cmae_Bot2011_COI	MA60	COI	45	BB74007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA60	ATGAACAGTCTATCCTCCTTTAG	151.17218
BB74007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78588	WGS84	BB74007	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74007_Msal_Bot2012_COI	MA60	1	BB74007_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74007	Sample	BB74007	targeted	Absent	Sample	Msal_Bot2012_COI	MA60	COI	45	BB74007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA60	GYTAGTTCCRATGATGTTAGCTG	151.17218
BB74009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81714	WGS84	BB74009	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.757	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74009_Aamu_Bax2006_COI	MA88	1	BB74009_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74009	Sample	BB74009	targeted	Present	Sample	Aamu_Bax2006_COI	MA88	COI	45	BB74009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA88	GCACAACCGGGATCTTTACTTC	144.92188
BB74009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81714	WGS84	BB74009	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74009_Pcan_Bot2011_IGS	MA88	1	BB74009_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74009	Sample	BB74009	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA88	IGS	45	BB74009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA88	CGTAATCCTCAGTACTGGCTA	144.92188
BB74009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81714	WGS84	BB74009	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.254	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74009_Sspa_Oph2007_COI	MA88	1	BB74009_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74009	Sample	BB74009	targeted	Present	Sample	Sspa_Oph2007_COI	MA88	COI	45	BB74009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA88	GAAGGCCTTCCGATGATC	144.92188
BB74009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81714	WGS84	BB74009	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74009_Upin_Bot2011_COI	MA88	1	BB74009_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74009	Sample	BB74009	targeted	Absent	Sample	Upin_Bot2011_COI	MA88	COI	45	BB74009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA88	CTTTAATTACAGCGTTTTTATTGTTGT	144.92188
BB74010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.794	WGS84	BB74010	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74010_Cmae_Bot2011_COI	MA45	1	BB74010_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74010	Sample	BB74010	targeted	Absent	Sample	Cmae_Bot2011_COI	MA45	COI	45	BB74010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA45	ATGAACAGTCTATCCTCCTTTAG	151.1844
BB74010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.794	WGS84	BB74010	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74010_Msal_Bot2012_COI	MA45	1	BB74010_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74010	Sample	BB74010	targeted	Absent	Sample	Msal_Bot2012_COI	MA45	COI	45	BB74010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA45	GYTAGTTCCRATGATGTTAGCTG	151.1844
BB74012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87588	WGS84	BB74012	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.282	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74012_Aamu_Bax2006_COI	HA6	1	BB74012_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74012	Sample	BB74012	targeted	Present	Sample	Aamu_Bax2006_COI	HA6	COI	45	BB74012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA6	GCACAACCGGGATCTTTACTTC	147.34299
BB74012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87588	WGS84	BB74012	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74012_Pcan_Bot2011_IGS	HA6	1	BB74012_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74012	Sample	BB74012	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA6	IGS	45	BB74012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA6	CGTAATCCTCAGTACTGGCTA	147.34299
BB74012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87588	WGS84	BB74012	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74012_Sspa_Oph2007_COI	HA6	1	BB74012_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74012	Sample	BB74012	targeted	Absent	Sample	Sspa_Oph2007_COI	HA6	COI	45	BB74012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA6	GAAGGCCTTCCGATGATC	147.34299
BB74012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87588	WGS84	BB74012	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74012_Upin_Bot2011_COI	HA6	1	BB74012_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74012	Sample	BB74012	targeted	Absent	Sample	Upin_Bot2011_COI	HA6	COI	45	BB74012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA6	CTTTAATTACAGCGTTTTTATTGTTGT	147.34299
BB74013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.87954	WGS84	BB74013	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74013_Mgig_Bot2012_COI	HA7	1	BB74013_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74013	Sample	BB74013	targeted	Absent	Sample	Mgig_Bot2012_COI	HA7	COI	45	BB74013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA7	TCTTATTCGTTGGAGACTTTATAACCCT	147.34488
BB74014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78625	WGS84	BB74014	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74014_Cmae_Bot2011_COI	GA57	1	BB74014_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74014	Sample	BB74014	targeted	Absent	Sample	Cmae_Bot2011_COI	GA57	COI	45	BB74014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA57	ATGAACAGTCTATCCTCCTTTAG	151.17631
BB74014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78625	WGS84	BB74014	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74014_Msal_Bot2012_COI	GA57	1	BB74014_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74014	Sample	BB74014	targeted	Absent	Sample	Msal_Bot2012_COI	GA57	COI	45	BB74014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA57	GYTAGTTCCRATGATGTTAGCTG	151.17631
BB74016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88575	WGS84	BB74016	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.541	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74016_Aamu_Bax2006_COI	HA8	1	BB74016_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74016	Sample	BB74016	targeted	Present	Sample	Aamu_Bax2006_COI	HA8	COI	45	BB74016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA8	GCACAACCGGGATCTTTACTTC	147.33759
BB74016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88575	WGS84	BB74016	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74016_Pcan_Bot2011_IGS	HA8	1	BB74016_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74016	Sample	BB74016	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA8	IGS	45	BB74016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA8	CGTAATCCTCAGTACTGGCTA	147.33759
BB74016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88575	WGS84	BB74016	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74016_Sspa_Oph2007_COI	HA8	1	BB74016_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74016	Sample	BB74016	targeted	Absent	Sample	Sspa_Oph2007_COI	HA8	COI	45	BB74016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA8	GAAGGCCTTCCGATGATC	147.33759
BB74016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88575	WGS84	BB74016	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74016_Upin_Bot2011_COI	HA8	1	BB74016_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74016	Sample	BB74016	targeted	Absent	Sample	Upin_Bot2011_COI	HA8	COI	45	BB74016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA8	CTTTAATTACAGCGTTTTTATTGTTGT	147.33759
BB74017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78949	WGS84	BB74017	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74017_Mgig_Bot2012_COI	GA53	1	BB74017_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74017	Sample	BB74017	targeted	Absent	Sample	Mgig_Bot2012_COI	GA53	COI	45	BB74017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA53	TCTTATTCGTTGGAGACTTTATAACCCT	151.17594
BB74018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88512	WGS84	BB74018	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74018_Cmae_Bot2011_COI	HA9	1	BB74018_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74018	Sample	BB74018	targeted	Absent	Sample	Cmae_Bot2011_COI	HA9	COI	45	BB74018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA9	ATGAACAGTCTATCCTCCTTTAG	147.33365
BB74018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88512	WGS84	BB74018	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74018_Msal_Bot2012_COI	HA9	1	BB74018_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74018	Sample	BB74018	targeted	Absent	Sample	Msal_Bot2012_COI	HA9	COI	45	BB74018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA9	GYTAGTTCCRATGATGTTAGCTG	147.33365
BB74020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79134	WGS84	BB74020	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74020_Mgig_Bot2012_COI	GA49	1	BB74020_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74020	Sample	BB74020	targeted	Absent	Sample	Mgig_Bot2012_COI	GA49	COI	45	BB74020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA49	TCTTATTCGTTGGAGACTTTATAACCCT	151.18417
BB74021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7912	WGS84	BB74021	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74021_Cmae_Bot2011_COI	GA48	1	BB74021_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74021	Sample	BB74021	targeted	Absent	Sample	Cmae_Bot2011_COI	GA48	COI	45	BB74021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA48	ATGAACAGTCTATCCTCCTTTAG	151.17933
BB74021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7912	WGS84	BB74021	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74021_Msal_Bot2012_COI	GA48	1	BB74021_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74021	Sample	BB74021	targeted	Absent	Sample	Msal_Bot2012_COI	GA48	COI	45	BB74021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA48	GYTAGTTCCRATGATGTTAGCTG	151.17933
BB74023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88275	WGS84	BB74023	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.283	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74023_Aamu_Bax2006_COI	HA12	1	BB74023_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74023	Sample	BB74023	targeted	Present	Sample	Aamu_Bax2006_COI	HA12	COI	45	BB74023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA12	GCACAACCGGGATCTTTACTTC	147.33625
BB74023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88275	WGS84	BB74023	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74023_Pcan_Bot2011_IGS	HA12	1	BB74023_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74023	Sample	BB74023	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA12	IGS	45	BB74023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA12	CGTAATCCTCAGTACTGGCTA	147.33625
BB74023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88275	WGS84	BB74023	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74023_Sspa_Oph2007_COI	HA12	1	BB74023_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74023	Sample	BB74023	targeted	Absent	Sample	Sspa_Oph2007_COI	HA12	COI	45	BB74023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA12	GAAGGCCTTCCGATGATC	147.33625
BB74023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.88275	WGS84	BB74023	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74023_Upin_Bot2011_COI	HA12	1	BB74023_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74023	Sample	BB74023	targeted	Absent	Sample	Upin_Bot2011_COI	HA12	COI	45	BB74023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA12	CTTTAATTACAGCGTTTTTATTGTTGT	147.33625
BB74024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78496	WGS84	BB74024	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74024_Mgig_Bot2012_COI	MA70	1	BB74024_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74024	Sample	BB74024	targeted	Absent	Sample	Mgig_Bot2012_COI	MA70	COI	45	BB74024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA70	TCTTATTCGTTGGAGACTTTATAACCCT	151.20333
BB74025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77966	WGS84	BB74025	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74025_Cmae_Bot2011_COI	MA65	1	BB74025_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74025	Sample	BB74025	targeted	Absent	Sample	Cmae_Bot2011_COI	MA65	COI	45	BB74025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA65	ATGAACAGTCTATCCTCCTTTAG	151.19784
BB74025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77966	WGS84	BB74025	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74025_Msal_Bot2012_COI	MA65	1	BB74025_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74025	Sample	BB74025	targeted	Absent	Sample	Msal_Bot2012_COI	MA65	COI	45	BB74025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA65	GYTAGTTCCRATGATGTTAGCTG	151.19784
BB74027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78359	WGS84	BB74027	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74027_Aamu_Bax2006_COI	GA68	1	BB74027_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74027	Sample	BB74027	targeted	Absent	Sample	Aamu_Bax2006_COI	GA68	COI	45	BB74027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA68	GCACAACCGGGATCTTTACTTC	151.20135
BB74027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78359	WGS84	BB74027	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74027_Pcan_Bot2011_IGS	GA68	1	BB74027_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74027	Sample	BB74027	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA68	IGS	45	BB74027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA68	CGTAATCCTCAGTACTGGCTA	151.20135
BB74027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78359	WGS84	BB74027	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74027_Sspa_Oph2007_COI	GA68	1	BB74027_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74027	Sample	BB74027	targeted	Absent	Sample	Sspa_Oph2007_COI	GA68	COI	45	BB74027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA68	GAAGGCCTTCCGATGATC	151.20135
BB74027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.78359	WGS84	BB74027	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74027_Upin_Bot2011_COI	GA68	1	BB74027_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74027	Sample	BB74027	targeted	Absent	Sample	Upin_Bot2011_COI	GA68	COI	45	BB74027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA68	CTTTAATTACAGCGTTTTTATTGTTGT	151.20135
BB74028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.7813	WGS84	BB74028	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74028_Mgig_Bot2012_COI	GA67	1	BB74028_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74028	Sample	BB74028	targeted	Absent	Sample	Mgig_Bot2012_COI	GA67	COI	45	BB74028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA67	TCTTATTCGTTGGAGACTTTATAACCCT	151.1994
BB74029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84711	WGS84	BB74029	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74029_Cmae_Bot2011_COI	HA16	1	BB74029_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74029	Sample	BB74029	targeted	Absent	Sample	Cmae_Bot2011_COI	HA16	COI	45	BB74029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA16	ATGAACAGTCTATCCTCCTTTAG	147.32992
BB74029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84711	WGS84	BB74029	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74029_Msal_Bot2012_COI	HA16	1	BB74029_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74029	Sample	BB74029	targeted	Absent	Sample	Msal_Bot2012_COI	HA16	COI	45	BB74029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA16	GYTAGTTCCRATGATGTTAGCTG	147.32992
BB74030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84551	WGS84	BB74030	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.308	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74030_Aamu_Bax2006_COI	HA17	1	BB74030_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74030	Sample	BB74030	targeted	Present	Sample	Aamu_Bax2006_COI	HA17	COI	45	BB74030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA17	GCACAACCGGGATCTTTACTTC	147.33086
BB74030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84551	WGS84	BB74030	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74030_Pcan_Bot2011_IGS	HA17	1	BB74030_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74030	Sample	BB74030	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA17	IGS	45	BB74030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA17	CGTAATCCTCAGTACTGGCTA	147.33086
BB74030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84551	WGS84	BB74030	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74030_Sspa_Oph2007_COI	HA17	1	BB74030_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74030	Sample	BB74030	targeted	Absent	Sample	Sspa_Oph2007_COI	HA17	COI	45	BB74030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA17	GAAGGCCTTCCGATGATC	147.33086
BB74030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84551	WGS84	BB74030	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74030_Upin_Bot2011_COI	HA17	1	BB74030_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74030	Sample	BB74030	targeted	Absent	Sample	Upin_Bot2011_COI	HA17	COI	45	BB74030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA17	CTTTAATTACAGCGTTTTTATTGTTGT	147.33086
BB74031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.77756	WGS84	BB74031	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74031_Mgig_Bot2012_COI	GA63	1	BB74031_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74031	Sample	BB74031	targeted	Absent	Sample	Mgig_Bot2012_COI	GA63	COI	45	BB74031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA63	TCTTATTCGTTGGAGACTTTATAACCCT	151.19502
BB74032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84416	WGS84	BB74032	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.737	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74032_Cmae_Bot2011_COI	HA18	1	BB74032_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74032	Sample	BB74032	targeted	Present	Sample	Cmae_Bot2011_COI	HA18	COI	45	BB74032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA18	ATGAACAGTCTATCCTCCTTTAG	147.32978
BB74032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84416	WGS84	BB74032	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74032_Msal_Bot2012_COI	HA18	1	BB74032_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74032	Sample	BB74032	targeted	Absent	Sample	Msal_Bot2012_COI	HA18	COI	45	BB74032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA18	GYTAGTTCCRATGATGTTAGCTG	147.32978
BB74034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8293	WGS84	BB74034	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.365	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74034_Aamu_Bax2006_COI	HA22	1	BB74034_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74034	Sample	BB74034	targeted	Present	Sample	Aamu_Bax2006_COI	HA22	COI	45	BB74034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA22	GCACAACCGGGATCTTTACTTC	147.3183
BB74034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8293	WGS84	BB74034	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74034_Pcan_Bot2011_IGS	HA22	1	BB74034_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74034	Sample	BB74034	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA22	IGS	45	BB74034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA22	CGTAATCCTCAGTACTGGCTA	147.3183
BB74034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8293	WGS84	BB74034	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74034_Sspa_Oph2007_COI	HA22	1	BB74034_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74034	Sample	BB74034	targeted	Absent	Sample	Sspa_Oph2007_COI	HA22	COI	45	BB74034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA22	GAAGGCCTTCCGATGATC	147.3183
BB74034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.8293	WGS84	BB74034	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74034_Upin_Bot2011_COI	HA22	1	BB74034_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74034	Sample	BB74034	targeted	Absent	Sample	Upin_Bot2011_COI	HA22	COI	45	BB74034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA22	CTTTAATTACAGCGTTTTTATTGTTGT	147.3183
BB74035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79115	WGS84	BB74035	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74035_Mgig_Bot2012_COI	GA72	1	BB74035_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74035	Sample	BB74035	targeted	Absent	Sample	Mgig_Bot2012_COI	GA72	COI	45	BB74035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA72	TCTTATTCGTTGGAGACTTTATAACCCT	151.2105
BB74036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79117	WGS84	BB74036	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74036_Cmae_Bot2011_COI	GA71	1	BB74036_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74036	Sample	BB74036	targeted	Absent	Sample	Cmae_Bot2011_COI	GA71	COI	45	BB74036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA71	ATGAACAGTCTATCCTCCTTTAG	151.21073
BB74036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.79117	WGS84	BB74036	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74036_Msal_Bot2012_COI	GA71	1	BB74036_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74036	Sample	BB74036	targeted	Absent	Sample	Msal_Bot2012_COI	GA71	COI	45	BB74036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA71	GYTAGTTCCRATGATGTTAGCTG	151.21073
BB74038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81951	WGS84	BB74038	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74038_Aamu_Bax2006_COI	MA105	1	BB74038_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74038	Sample	BB74038	targeted	Absent	Sample	Aamu_Bax2006_COI	MA105	COI	45	BB74038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA105	GCACAACCGGGATCTTTACTTC	151.24366
BB74038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81951	WGS84	BB74038	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74038_Pcan_Bot2011_IGS	MA105	1	BB74038_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74038	Sample	BB74038	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA105	IGS	45	BB74038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA105	CGTAATCCTCAGTACTGGCTA	151.24366
BB74038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81951	WGS84	BB74038	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74038_Sspa_Oph2007_COI	MA105	1	BB74038_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74038	Sample	BB74038	targeted	Absent	Sample	Sspa_Oph2007_COI	MA105	COI	45	BB74038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA105	GAAGGCCTTCCGATGATC	151.24366
BB74038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81951	WGS84	BB74038	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74038_Upin_Bot2011_COI	MA105	1	BB74038_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74038	Sample	BB74038	targeted	Absent	Sample	Upin_Bot2011_COI	MA105	COI	45	BB74038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA105	CTTTAATTACAGCGTTTTTATTGTTGT	151.24366
BB74039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.83217	WGS84	BB74039	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74039_Mgig_Bot2012_COI	HA24	1	BB74039_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74039	Sample	BB74039	targeted	Absent	Sample	Mgig_Bot2012_COI	HA24	COI	45	BB74039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA24	TCTTATTCGTTGGAGACTTTATAACCCT	147.32211
BB74041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82257	WGS84	BB74041	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74041_Aamu_Bax2006_COI	MA90	1	BB74041_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74041	Sample	BB74041	targeted	Absent	Sample	Aamu_Bax2006_COI	MA90	COI	45	BB74041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA90	GCACAACCGGGATCTTTACTTC	151.24362
BB74041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82257	WGS84	BB74041	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74041_Pcan_Bot2011_IGS	MA90	1	BB74041_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74041	Sample	BB74041	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA90	IGS	45	BB74041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA90	CGTAATCCTCAGTACTGGCTA	151.24362
BB74041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82257	WGS84	BB74041	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74041_Sspa_Oph2007_COI	MA90	1	BB74041_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74041	Sample	BB74041	targeted	Absent	Sample	Sspa_Oph2007_COI	MA90	COI	45	BB74041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA90	GAAGGCCTTCCGATGATC	151.24362
BB74041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82257	WGS84	BB74041	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74041_Upin_Bot2011_COI	MA90	1	BB74041_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74041	Sample	BB74041	targeted	Absent	Sample	Upin_Bot2011_COI	MA90	COI	45	BB74041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA90	CTTTAATTACAGCGTTTTTATTGTTGT	151.24362
BB74042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82275	WGS84	BB74042	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74042_Mgig_Bot2012_COI	MA71	1	BB74042_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74042	Sample	BB74042	targeted	Absent	Sample	Mgig_Bot2012_COI	MA71	COI	45	BB74042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA71	TCTTATTCGTTGGAGACTTTATAACCCT	144.90789
BB74043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82556	WGS84	BB74043	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74043_Cmae_Bot2011_COI	MA63	1	BB74043_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74043	Sample	BB74043	targeted	Absent	Sample	Cmae_Bot2011_COI	MA63	COI	45	BB74043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA63	ATGAACAGTCTATCCTCCTTTAG	144.90337
BB74043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82556	WGS84	BB74043	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74043_Msal_Bot2012_COI	MA63	1	BB74043_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74043	Sample	BB74043	targeted	Absent	Sample	Msal_Bot2012_COI	MA63	COI	45	BB74043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA63	GYTAGTTCCRATGATGTTAGCTG	144.90337
BB74045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81955	WGS84	BB74045	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.969	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74045_Aamu_Bax2006_COI	MA91	1	BB74045_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74045	Sample	BB74045	targeted	Present	Sample	Aamu_Bax2006_COI	MA91	COI	45	BB74045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA91	GCACAACCGGGATCTTTACTTC	144.92944
BB74045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81955	WGS84	BB74045	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74045_Pcan_Bot2011_IGS	MA91	1	BB74045_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74045	Sample	BB74045	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA91	IGS	45	BB74045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA91	CGTAATCCTCAGTACTGGCTA	144.92944
BB74045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81955	WGS84	BB74045	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.344	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74045_Sspa_Oph2007_COI	MA91	1	BB74045_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74045	Sample	BB74045	targeted	Present	Sample	Sspa_Oph2007_COI	MA91	COI	45	BB74045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA91	GAAGGCCTTCCGATGATC	144.92944
BB74045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81955	WGS84	BB74045	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74045_Upin_Bot2011_COI	MA91	1	BB74045_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74045	Sample	BB74045	targeted	Absent	Sample	Upin_Bot2011_COI	MA91	COI	45	BB74045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA91	CTTTAATTACAGCGTTTTTATTGTTGT	144.92944
BB74046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81672	WGS84	BB74046	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74046_Mgig_Bot2012_COI	GA107	1	BB74046_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74046	Sample	BB74046	targeted	Absent	Sample	Mgig_Bot2012_COI	GA107	COI	45	BB74046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA107	TCTTATTCGTTGGAGACTTTATAACCCT	151.24042
BB74047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8179	WGS84	BB74047	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74047_Cmae_Bot2011_COI	GA106	1	BB74047_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74047	Sample	BB74047	targeted	Absent	Sample	Cmae_Bot2011_COI	GA106	COI	45	BB74047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA106	ATGAACAGTCTATCCTCCTTTAG	151.24243
BB74047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8179	WGS84	BB74047	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74047_Msal_Bot2012_COI	GA106	1	BB74047_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74047	Sample	BB74047	targeted	Absent	Sample	Msal_Bot2012_COI	GA106	COI	45	BB74047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA106	GYTAGTTCCRATGATGTTAGCTG	151.24243
BB74049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82484	WGS84	BB74049	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.666	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74049_Aamu_Bax2006_COI	MA62	1	BB74049_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74049	Sample	BB74049	targeted	Present	Sample	Aamu_Bax2006_COI	MA62	COI	45	BB74049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA62	GCACAACCGGGATCTTTACTTC	144.90426
BB74049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82484	WGS84	BB74049	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74049_Pcan_Bot2011_IGS	MA62	1	BB74049_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74049	Sample	BB74049	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA62	IGS	45	BB74049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA62	CGTAATCCTCAGTACTGGCTA	144.90426
BB74049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82484	WGS84	BB74049	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.504	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74049_Sspa_Oph2007_COI	MA62	1	BB74049_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74049	Sample	BB74049	targeted	Present	Sample	Sspa_Oph2007_COI	MA62	COI	45	BB74049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA62	GAAGGCCTTCCGATGATC	144.90426
BB74049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82484	WGS84	BB74049	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74049_Upin_Bot2011_COI	MA62	1	BB74049_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74049	Sample	BB74049	targeted	Absent	Sample	Upin_Bot2011_COI	MA62	COI	45	BB74049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA62	CTTTAATTACAGCGTTTTTATTGTTGT	144.90426
BB74050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81682	WGS84	BB74050	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74050_Cmae_Bot2011_COI	GA101	1	BB74050_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74050	Sample	BB74050	targeted	Absent	Sample	Cmae_Bot2011_COI	GA101	COI	45	BB74050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA101	ATGAACAGTCTATCCTCCTTTAG	151.23872
BB74050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81682	WGS84	BB74050	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74050_Msal_Bot2012_COI	GA101	1	BB74050_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74050	Sample	BB74050	targeted	Absent	Sample	Msal_Bot2012_COI	GA101	COI	45	BB74050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA101	GYTAGTTCCRATGATGTTAGCTG	151.23872
BB74052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84447	WGS84	BB74052	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74052_Aamu_Bax2006_COI	HA20	1	BB74052_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74052	Sample	BB74052	targeted	Absent	Sample	Aamu_Bax2006_COI	HA20	COI	45	BB74052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA20	GCACAACCGGGATCTTTACTTC	147.32885
BB74052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84447	WGS84	BB74052	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74052_Pcan_Bot2011_IGS	HA20	1	BB74052_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74052	Sample	BB74052	targeted	Absent	Sample	Pcan_Bot2011_IGS	HA20	IGS	45	BB74052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA20	CGTAATCCTCAGTACTGGCTA	147.32885
BB74052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84447	WGS84	BB74052	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74052_Sspa_Oph2007_COI	HA20	1	BB74052_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74052	Sample	BB74052	targeted	Absent	Sample	Sspa_Oph2007_COI	HA20	COI	45	BB74052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA20	GAAGGCCTTCCGATGATC	147.32885
BB74052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10059	-42.84447	WGS84	BB74052	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74052_Upin_Bot2011_COI	HA20	1	BB74052_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74052	Sample	BB74052	targeted	Absent	Sample	Upin_Bot2011_COI	HA20	COI	45	BB74052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HA20	CTTTAATTACAGCGTTTTTATTGTTGT	147.32885
BB74053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82761	WGS84	BB74053	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74053_Mgig_Bot2012_COI	GA97	1	BB74053_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74053	Sample	BB74053	targeted	Absent	Sample	Mgig_Bot2012_COI	GA97	COI	45	BB74053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA97	TCTTATTCGTTGGAGACTTTATAACCCT	151.24723
BB74054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82638	WGS84	BB74054	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74054_Cmae_Bot2011_COI	GA96	1	BB74054_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74054	Sample	BB74054	targeted	Absent	Sample	Cmae_Bot2011_COI	GA96	COI	45	BB74054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA96	ATGAACAGTCTATCCTCCTTTAG	151.24668
BB74054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82638	WGS84	BB74054	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74054_Msal_Bot2012_COI	GA96	1	BB74054_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74054	Sample	BB74054	targeted	Absent	Sample	Msal_Bot2012_COI	GA96	COI	45	BB74054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA96	GYTAGTTCCRATGATGTTAGCTG	151.24668
BB74056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82353	WGS84	BB74056	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74056_Aamu_Bax2006_COI	GA93	1	BB74056_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74056	Sample	BB74056	targeted	Absent	Sample	Aamu_Bax2006_COI	GA93	COI	45	BB74056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA93	GCACAACCGGGATCTTTACTTC	151.24481
BB74056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82353	WGS84	BB74056	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74056_Pcan_Bot2011_IGS	GA93	1	BB74056_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74056	Sample	BB74056	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA93	IGS	45	BB74056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA93	CGTAATCCTCAGTACTGGCTA	151.24481
BB74056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82353	WGS84	BB74056	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74056_Sspa_Oph2007_COI	GA93	1	BB74056_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74056	Sample	BB74056	targeted	Absent	Sample	Sspa_Oph2007_COI	GA93	COI	45	BB74056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA93	GAAGGCCTTCCGATGATC	151.24481
BB74056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82353	WGS84	BB74056	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74056_Upin_Bot2011_COI	GA93	1	BB74056_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74056	Sample	BB74056	targeted	Absent	Sample	Upin_Bot2011_COI	GA93	COI	45	BB74056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA93	CTTTAATTACAGCGTTTTTATTGTTGT	151.24481
BB74057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82383	WGS84	BB74057	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74057_Mgig_Bot2012_COI	GA92	1	BB74057_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74057	Sample	BB74057	targeted	Absent	Sample	Mgig_Bot2012_COI	GA92	COI	45	BB74057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA92	TCTTATTCGTTGGAGACTTTATAACCCT	151.24413
BB74058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82347	WGS84	BB74058	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74058_Cmae_Bot2011_COI	GA91	1	BB74058_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74058	Sample	BB74058	targeted	Absent	Sample	Cmae_Bot2011_COI	GA91	COI	45	BB74058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA91	ATGAACAGTCTATCCTCCTTTAG	151.24428
BB74058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82347	WGS84	BB74058	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74058_Msal_Bot2012_COI	GA91	1	BB74058_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74058	Sample	BB74058	targeted	Absent	Sample	Msal_Bot2012_COI	GA91	COI	45	BB74058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA91	GYTAGTTCCRATGATGTTAGCTG	151.24428
BB74060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82381	WGS84	BB74060	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74060_Mgig_Bot2012_COI	GA88	1	BB74060_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74060	Sample	BB74060	targeted	Absent	Sample	Mgig_Bot2012_COI	GA88	COI	45	BB74060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA88	TCTTATTCGTTGGAGACTTTATAACCCT	151.24304
BB74061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82289	WGS84	BB74061	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74061_Cmae_Bot2011_COI	GA87	1	BB74061_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74061	Sample	BB74061	targeted	Absent	Sample	Cmae_Bot2011_COI	GA87	COI	45	BB74061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA87	ATGAACAGTCTATCCTCCTTTAG	151.24174
BB74061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82289	WGS84	BB74061	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74061_Msal_Bot2012_COI	GA87	1	BB74061_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74061	Sample	BB74061	targeted	Absent	Sample	Msal_Bot2012_COI	GA87	COI	45	BB74061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA87	GYTAGTTCCRATGATGTTAGCTG	151.24174
BB74063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8412	WGS84	BB74063	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.35	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74063_Aamu_Bax2006_COI	MA38	1	BB74063_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74063	Sample	BB74063	targeted	Present	Sample	Aamu_Bax2006_COI	MA38	COI	45	BB74063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA38	GCACAACCGGGATCTTTACTTC	144.90371
BB74063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8412	WGS84	BB74063	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74063_Pcan_Bot2011_IGS	MA38	1	BB74063_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74063	Sample	BB74063	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA38	IGS	45	BB74063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA38	CGTAATCCTCAGTACTGGCTA	144.90371
BB74063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8412	WGS84	BB74063	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.521	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74063_Sspa_Oph2007_COI	MA38	1	BB74063_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74063	Sample	BB74063	targeted	Present	Sample	Sspa_Oph2007_COI	MA38	COI	45	BB74063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA38	GAAGGCCTTCCGATGATC	144.90371
BB74063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8412	WGS84	BB74063	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.544	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74063_Upin_Bot2011_COI	MA38	1	BB74063_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74063	Sample	BB74063	targeted	Present	Sample	Upin_Bot2011_COI	MA38	COI	45	BB74063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA38	CTTTAATTACAGCGTTTTTATTGTTGT	144.90371
BB74064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82104	WGS84	BB74064	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74064_Mgig_Bot2012_COI	GA83	1	BB74064_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74064	Sample	BB74064	targeted	Absent	Sample	Mgig_Bot2012_COI	GA83	COI	45	BB74064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA83	TCTTATTCGTTGGAGACTTTATAACCCT	151.24161
BB74065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82039	WGS84	BB74065	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74065_Cmae_Bot2011_COI	GA82	1	BB74065_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74065	Sample	BB74065	targeted	Absent	Sample	Cmae_Bot2011_COI	GA82	COI	45	BB74065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA82	ATGAACAGTCTATCCTCCTTTAG	151.24193
BB74065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82039	WGS84	BB74065	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74065_Msal_Bot2012_COI	GA82	1	BB74065_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74065	Sample	BB74065	targeted	Absent	Sample	Msal_Bot2012_COI	GA82	COI	45	BB74065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA82	GYTAGTTCCRATGATGTTAGCTG	151.24193
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.38	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74067_Aamu_Bax2006_COI	MA98	1	BB74067_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Present	Sample	Aamu_Bax2006_COI	MA98	COI	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	GCACAACCGGGATCTTTACTTC	144.92884
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74067_Pcan_Bot2011_IGS	MA98	1	BB74067_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA98	IGS	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	CGTAATCCTCAGTACTGGCTA	144.92884
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.268	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74067_Sspa_Oph2007_COI	MA98	1	BB74067_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Present	Sample	Sspa_Oph2007_COI	MA98	COI	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	GAAGGCCTTCCGATGATC	144.92884
BB74067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81819	WGS84	BB74067	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74067_Upin_Bot2011_COI	MA98	1	BB74067_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74067	Sample	BB74067	targeted	Absent	Sample	Upin_Bot2011_COI	MA98	COI	45	BB74067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA98	CTTTAATTACAGCGTTTTTATTGTTGT	144.92884
BB74068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82335	WGS84	BB74068	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74068_Mgig_Bot2012_COI	MA69	1	BB74068_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74068	Sample	BB74068	targeted	Absent	Sample	Mgig_Bot2012_COI	MA69	COI	45	BB74068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA69	TCTTATTCGTTGGAGACTTTATAACCCT	144.90659
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74069_Cmae_Bot2011_COI	MA72	1	BB74069_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Absent	Sample	Cmae_Bot2011_COI	MA72	COI	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	ATGAACAGTCTATCCTCCTTTAG	144.90996
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB74069_Hsan_Wil2021_COI	MA72	1	BB74069_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Absent	Sample	Hsan_Wil2021_COI	MA72	COI	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	CCTGGGCCGGTATAGTAGGT	144.90996
BB74069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82205	WGS84	BB74069	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74069_Msal_Bot2012_COI	MA72	1	BB74069_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74069	Sample	BB74069	targeted	Absent	Sample	Msal_Bot2012_COI	MA72	COI	45	BB74069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA72	GYTAGTTCCRATGATGTTAGCTG	144.90996
BB74070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82055	WGS84	BB74070	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74070_Aamu_Bax2006_COI	GA19	1	BB74070_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74070	Sample	BB74070	targeted	Absent	Sample	Aamu_Bax2006_COI	GA19	COI	45	BB74070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA19	GCACAACCGGGATCTTTACTTC	151.23966
BB74070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82055	WGS84	BB74070	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74070_Pcan_Bot2011_IGS	GA19	1	BB74070_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74070	Sample	BB74070	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA19	IGS	45	BB74070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA19	CGTAATCCTCAGTACTGGCTA	151.23966
BB74070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82055	WGS84	BB74070	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74070_Sspa_Oph2007_COI	GA19	1	BB74070_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74070	Sample	BB74070	targeted	Absent	Sample	Sspa_Oph2007_COI	GA19	COI	45	BB74070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA19	GAAGGCCTTCCGATGATC	151.23966
BB74070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.82055	WGS84	BB74070	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74070_Upin_Bot2011_COI	GA19	1	BB74070_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74070	Sample	BB74070	targeted	Absent	Sample	Upin_Bot2011_COI	GA19	COI	45	BB74070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA19	CTTTAATTACAGCGTTTTTATTGTTGT	151.23966
BB74071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81913	WGS84	BB74071	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74071_Mgig_Bot2012_COI	GA18	1	BB74071_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74071	Sample	BB74071	targeted	Absent	Sample	Mgig_Bot2012_COI	GA18	COI	45	BB74071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA18	TCTTATTCGTTGGAGACTTTATAACCCT	151.23931
BB74072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81501	WGS84	BB74072	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74072_Cmae_Bot2011_COI	GA17	1	BB74072_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74072	Sample	BB74072	targeted	Absent	Sample	Cmae_Bot2011_COI	GA17	COI	45	BB74072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA17	ATGAACAGTCTATCCTCCTTTAG	151.23683
BB74072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81501	WGS84	BB74072	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74072_Msal_Bot2012_COI	GA17	1	BB74072_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74072	Sample	BB74072	targeted	Absent	Sample	Msal_Bot2012_COI	GA17	COI	45	BB74072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA17	GYTAGTTCCRATGATGTTAGCTG	151.23683
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	21.808	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74074_Aamu_Bax2006_COI	MA64	1	BB74074_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Present	Sample	Aamu_Bax2006_COI	MA64	COI	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	GCACAACCGGGATCTTTACTTC	144.92213
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74074_Pcan_Bot2011_IGS	MA64	1	BB74074_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA64	IGS	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	CGTAATCCTCAGTACTGGCTA	144.92213
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.211	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74074_Sspa_Oph2007_COI	MA64	1	BB74074_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Present	Sample	Sspa_Oph2007_COI	MA64	COI	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	GAAGGCCTTCCGATGATC	144.92213
BB74074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.81987	WGS84	BB74074	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74074_Upin_Bot2011_COI	MA64	1	BB74074_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74074	Sample	BB74074	targeted	Absent	Sample	Upin_Bot2011_COI	MA64	COI	45	BB74074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA64	CTTTAATTACAGCGTTTTTATTGTTGT	144.92213
BB74075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.81598	WGS84	BB74075	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74075_Mgig_Bot2012_COI	GA13	1	BB74075_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74075	Sample	BB74075	targeted	Absent	Sample	Mgig_Bot2012_COI	GA13	COI	45	BB74075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA13	TCTTATTCGTTGGAGACTTTATAACCCT	151.23583
BB74076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83155	WGS84	BB74076	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74076_Cmae_Bot2011_COI	MA75	1	BB74076_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74076	Sample	BB74076	targeted	Absent	Sample	Cmae_Bot2011_COI	MA75	COI	45	BB74076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA75	ATGAACAGTCTATCCTCCTTTAG	151.25742
BB74076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83155	WGS84	BB74076	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74076_Msal_Bot2012_COI	MA75	1	BB74076_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74076	Sample	BB74076	targeted	Absent	Sample	Msal_Bot2012_COI	MA75	COI	45	BB74076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA75	GYTAGTTCCRATGATGTTAGCTG	151.25742
BB74078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83245	WGS84	BB74078	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74078_Aamu_Bax2006_COI	GA78	1	BB74078_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74078	Sample	BB74078	targeted	Absent	Sample	Aamu_Bax2006_COI	GA78	COI	45	BB74078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA78	GCACAACCGGGATCTTTACTTC	151.26325
BB74078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83245	WGS84	BB74078	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74078_Pcan_Bot2011_IGS	GA78	1	BB74078_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74078	Sample	BB74078	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA78	IGS	45	BB74078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA78	CGTAATCCTCAGTACTGGCTA	151.26325
BB74078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83245	WGS84	BB74078	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74078_Sspa_Oph2007_COI	GA78	1	BB74078_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74078	Sample	BB74078	targeted	Absent	Sample	Sspa_Oph2007_COI	GA78	COI	45	BB74078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA78	GAAGGCCTTCCGATGATC	151.26325
BB74078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83245	WGS84	BB74078	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74078_Upin_Bot2011_COI	GA78	1	BB74078_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74078	Sample	BB74078	targeted	Absent	Sample	Upin_Bot2011_COI	GA78	COI	45	BB74078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA78	CTTTAATTACAGCGTTTTTATTGTTGT	151.26325
BB74079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83171	WGS84	BB74079	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74079_Mgig_Bot2012_COI	GA77	1	BB74079_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74079	Sample	BB74079	targeted	Absent	Sample	Mgig_Bot2012_COI	GA77	COI	45	BB74079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA77	TCTTATTCGTTGGAGACTTTATAACCCT	151.262
BB74081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83131	WGS84	BB74081	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74081_Aamu_Bax2006_COI	GA74	1	BB74081_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74081	Sample	BB74081	targeted	Absent	Sample	Aamu_Bax2006_COI	GA74	COI	45	BB74081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA74	GCACAACCGGGATCTTTACTTC	151.2555
BB74081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83131	WGS84	BB74081	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74081_Pcan_Bot2011_IGS	GA74	1	BB74081_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74081	Sample	BB74081	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA74	IGS	45	BB74081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA74	CGTAATCCTCAGTACTGGCTA	151.2555
BB74081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83131	WGS84	BB74081	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74081_Sspa_Oph2007_COI	GA74	1	BB74081_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74081	Sample	BB74081	targeted	Absent	Sample	Sspa_Oph2007_COI	GA74	COI	45	BB74081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA74	GAAGGCCTTCCGATGATC	151.2555
BB74081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83131	WGS84	BB74081	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74081_Upin_Bot2011_COI	GA74	1	BB74081_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74081	Sample	BB74081	targeted	Absent	Sample	Upin_Bot2011_COI	GA74	COI	45	BB74081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA74	CTTTAATTACAGCGTTTTTATTGTTGT	151.2555
BB74082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.82014	WGS84	BB74082	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.764	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74082_Mgig_Bot2012_COI	MA106	1	BB74082_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74082	Sample	BB74082	targeted	Present	Sample	Mgig_Bot2012_COI	MA106	COI	45	BB74082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA106	TCTTATTCGTTGGAGACTTTATAACCCT	144.91161
BB74083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83492	WGS84	BB74083	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74083_Cmae_Bot2011_COI	GA43	1	BB74083_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74083	Sample	BB74083	targeted	Absent	Sample	Cmae_Bot2011_COI	GA43	COI	45	BB74083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA43	ATGAACAGTCTATCCTCCTTTAG	151.26834
BB74083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83492	WGS84	BB74083	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74083_Msal_Bot2012_COI	GA43	1	BB74083_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74083	Sample	BB74083	targeted	Absent	Sample	Msal_Bot2012_COI	GA43	COI	45	BB74083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA43	GYTAGTTCCRATGATGTTAGCTG	151.26834
BB74085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83556	WGS84	BB74085	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74085_Aamu_Bax2006_COI	GA41	1	BB74085_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74085	Sample	BB74085	targeted	Absent	Sample	Aamu_Bax2006_COI	GA41	COI	45	BB74085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA41	GCACAACCGGGATCTTTACTTC	151.27013
BB74085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83556	WGS84	BB74085	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74085_Pcan_Bot2011_IGS	GA41	1	BB74085_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74085	Sample	BB74085	targeted	Absent	Sample	Pcan_Bot2011_IGS	GA41	IGS	45	BB74085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA41	CGTAATCCTCAGTACTGGCTA	151.27013
BB74085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83556	WGS84	BB74085	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74085_Sspa_Oph2007_COI	GA41	1	BB74085_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74085	Sample	BB74085	targeted	Absent	Sample	Sspa_Oph2007_COI	GA41	COI	45	BB74085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA41	GAAGGCCTTCCGATGATC	151.27013
BB74085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.83556	WGS84	BB74085	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74085_Upin_Bot2011_COI	GA41	1	BB74085_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74085	Sample	BB74085	targeted	Absent	Sample	Upin_Bot2011_COI	GA41	COI	45	BB74085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA41	CTTTAATTACAGCGTTTTTATTGTTGT	151.27013
BB74086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10064	-37.8198	WGS84	BB74086	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74086_Mgig_Bot2012_COI	MA53	1	BB74086_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74086	Sample	BB74086	targeted	Absent	Sample	Mgig_Bot2012_COI	MA53	COI	45	BB74086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA53	TCTTATTCGTTGGAGACTTTATAACCCT	144.92399
BB74087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8363	WGS84	BB74087	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74087_Cmae_Bot2011_COI	GA38	1	BB74087_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74087	Sample	BB74087	targeted	Absent	Sample	Cmae_Bot2011_COI	GA38	COI	45	BB74087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA38	ATGAACAGTCTATCCTCCTTTAG	151.27496
BB74087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10057	-23.8363	WGS84	BB74087	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB74087_Msal_Bot2012_COI	GA38	1	BB74087_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74087	Sample	BB74087	targeted	Absent	Sample	Msal_Bot2012_COI	GA38	COI	45	BB74087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA38	GYTAGTTCCRATGATGTTAGCTG	151.27496
BB74089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44344	WGS84	BB74089	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74089_Aamu_Bax2006_COI	BA2	1	BB74089_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74089	Sample	BB74089	targeted	Absent	Sample	Aamu_Bax2006_COI	BA2	COI	45	BB74089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA2	GCACAACCGGGATCTTTACTTC	153.07422
BB74089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44344	WGS84	BB74089	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74089_Pcan_Bot2011_IGS	BA2	1	BB74089_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74089	Sample	BB74089	targeted	Absent	Sample	Pcan_Bot2011_IGS	BA2	IGS	45	BB74089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA2	CGTAATCCTCAGTACTGGCTA	153.07422
BB74089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44344	WGS84	BB74089	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74089_Sspa_Oph2007_COI	BA2	1	BB74089_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74089	Sample	BB74089	targeted	Absent	Sample	Sspa_Oph2007_COI	BA2	COI	45	BB74089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA2	GAAGGCCTTCCGATGATC	153.07422
BB74089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10058	-27.44344	WGS84	BB74089	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Winter	14/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74089_Upin_Bot2011_COI	BA2	1	BB74089_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74089	Sample	BB74089	targeted	Absent	Sample	Upin_Bot2011_COI	BA2	COI	45	BB74089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BA2	CTTTAATTACAGCGTTTTTATTGTTGT	153.07422
BB74188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85433	WGS84	BB74188	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74188_Aamu_Bax2006_COI	T1-09	1	BB74188_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74188	Sample	BB74188	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-09	COI	45	BB74188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-09	GCACAACCGGGATCTTTACTTC	147.87329
BB74188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85433	WGS84	BB74188	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74188_Pcan_Bot2011_IGS	T1-09	1	BB74188_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74188	Sample	BB74188	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-09	IGS	45	BB74188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-09	CGTAATCCTCAGTACTGGCTA	147.87329
BB74188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85433	WGS84	BB74188	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74188_Sspa_Oph2007_COI	T1-09	1	BB74188_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74188	Sample	BB74188	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-09	COI	45	BB74188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-09	GAAGGCCTTCCGATGATC	147.87329
BB74188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85433	WGS84	BB74188	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.546	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74188_Upin_Bot2011_COI	T1-09	1	BB74188_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74188	Sample	BB74188	targeted	Present	Sample	Upin_Bot2011_COI	T1-09	COI	45	BB74188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.87329
BB74189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85804	WGS84	BB74189	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74189_Cjap_Sim2018_COI	T1-17	1	BB74189_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74189	Sample	BB74189	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-17	COI	45	BB74189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.86795
BB74189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85804	WGS84	BB74189	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.133	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74189_Mgig_Bot2012_COI	T1-17	1	BB74189_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74189	Sample	BB74189	targeted	Present	Sample	Mgig_Bot2012_COI	T1-17	COI	45	BB74189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-17	TCTTATTCGTTGGAGACTTTATAACCCT	147.86795
BB74191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96918	WGS84	BB74191	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74191_Aamu_Bax2006_COI	T1-10	1	BB74191_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74191	Sample	BB74191	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-10	COI	45	BB74191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-10	GCACAACCGGGATCTTTACTTC	147.51603
BB74191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96918	WGS84	BB74191	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74191_Pcan_Bot2011_IGS	T1-10	1	BB74191_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74191	Sample	BB74191	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-10	IGS	45	BB74191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-10	CGTAATCCTCAGTACTGGCTA	147.51603
BB74191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96918	WGS84	BB74191	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74191_Sspa_Oph2007_COI	T1-10	1	BB74191_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74191	Sample	BB74191	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-10	COI	45	BB74191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-10	GAAGGCCTTCCGATGATC	147.51603
BB74191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96918	WGS84	BB74191	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74191_Upin_Bot2011_COI	T1-10	1	BB74191_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74191	Sample	BB74191	targeted	Absent	Sample	Upin_Bot2011_COI	T1-10	COI	45	BB74191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.51603
BB74192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96019	WGS84	BB74192	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74192_Cjap_Sim2018_COI	T1-18	1	BB74192_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74192	Sample	BB74192	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-18	COI	45	BB74192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52897
BB74192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96019	WGS84	BB74192	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.965	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74192_Mgig_Bot2012_COI	T1-18	1	BB74192_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74192	Sample	BB74192	targeted	Present	Sample	Mgig_Bot2012_COI	T1-18	COI	45	BB74192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-18	TCTTATTCGTTGGAGACTTTATAACCCT	147.52897
BB74193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88525	WGS84	BB74193	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74193_Cmae_Bot2011_COI	T1-03	1	BB74193_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74193	Sample	BB74193	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-03	COI	45	BB74193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-03	ATGAACAGTCTATCCTCCTTTAG	147.3358
BB74195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88393	WGS84	BB74195	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74195_Aamu_Bax2006_COI	T1-19	1	BB74195_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74195	Sample	BB74195	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-19	COI	45	BB74195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-19	GCACAACCGGGATCTTTACTTC	147.33802
BB74195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88393	WGS84	BB74195	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74195_Pcan_Bot2011_IGS	T1-19	1	BB74195_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74195	Sample	BB74195	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-19	IGS	45	BB74195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-19	CGTAATCCTCAGTACTGGCTA	147.33802
BB74195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88393	WGS84	BB74195	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74195_Sspa_Oph2007_COI	T1-19	1	BB74195_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74195	Sample	BB74195	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-19	COI	45	BB74195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-19	GAAGGCCTTCCGATGATC	147.33802
BB74195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88393	WGS84	BB74195	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74195_Upin_Bot2011_COI	T1-19	1	BB74195_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74195	Sample	BB74195	targeted	Absent	Sample	Upin_Bot2011_COI	T1-19	COI	45	BB74195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-19	CTTTAATTACAGCGTTTTTATTGTTGT	147.33802
BB74196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87764	WGS84	BB74196	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74196_Cjap_Sim2018_COI	T1-04	1	BB74196_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74196	Sample	BB74196	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-04	COI	45	BB74196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34464
BB74196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87764	WGS84	BB74196	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.498	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74196_Mgig_Bot2012_COI	T1-04	1	BB74196_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74196	Sample	BB74196	targeted	Present	Sample	Mgig_Bot2012_COI	T1-04	COI	45	BB74196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.34464
BB74197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82601	WGS84	BB74197	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74197_Cmae_Bot2011_COI	T1-12	1	BB74197_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74197	Sample	BB74197	targeted	Absent	Sample	Cmae_Bot2011_COI	T1-12	COI	45	BB74197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-12	ATGAACAGTCTATCCTCCTTTAG	147.30451
BB74199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84579	WGS84	BB74199	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74199_Aamu_Bax2006_COI	S2-03	1	BB74199_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74199	Sample	BB74199	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-03	COI	45	BB74199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-03	GCACAACCGGGATCTTTACTTC	138.49682
BB74199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84579	WGS84	BB74199	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74199_Pcan_Bot2011_IGS	S2-03	1	BB74199_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74199	Sample	BB74199	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-03	IGS	45	BB74199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-03	CGTAATCCTCAGTACTGGCTA	138.49682
BB74199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84579	WGS84	BB74199	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.873	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74199_Sspa_Oph2007_COI	S2-03	1	BB74199_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74199	Sample	BB74199	targeted	Present	Sample	Sspa_Oph2007_COI	S2-03	COI	45	BB74199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-03	GAAGGCCTTCCGATGATC	138.49682
BB74199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84579	WGS84	BB74199	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74199_Upin_Bot2011_COI	S2-03	1	BB74199_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74199	Sample	BB74199	targeted	Absent	Sample	Upin_Bot2011_COI	S2-03	COI	45	BB74199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.49682
BB74200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84483	WGS84	BB74200	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74200_Cjap_Sim2018_COI	S2-05	1	BB74200_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74200	Sample	BB74200	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-05	COI	45	BB74200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49685
BB74200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84483	WGS84	BB74200	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74200_Mgig_Bot2012_COI	S2-05	1	BB74200_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74200	Sample	BB74200	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-05	COI	45	BB74200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.49685
BB74201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83684	WGS84	BB74201	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74201_Cmae_Bot2011_COI	S2-08	1	BB74201_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74201	Sample	BB74201	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-08	COI	45	BB74201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-08	ATGAACAGTCTATCCTCCTTTAG	138.50934
BB74203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83626	WGS84	BB74203	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB74203_Aamu_Bax2006_COI	S2-10	1	BB74203_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74203	Sample	BB74203	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-10	COI	45	BB74203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-10	GCACAACCGGGATCTTTACTTC	138.50837
BB74203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83626	WGS84	BB74203	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB74203_Pcan_Bot2011_IGS	S2-10	1	BB74203_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74203	Sample	BB74203	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-10	IGS	45	BB74203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-10	CGTAATCCTCAGTACTGGCTA	138.50837
BB74203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83626	WGS84	BB74203	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.494	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB74203_Sspa_Oph2007_COI	S2-10	1	BB74203_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74203	Sample	BB74203	targeted	Present	Sample	Sspa_Oph2007_COI	S2-10	COI	45	BB74203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-10	GAAGGCCTTCCGATGATC	138.50837
BB74203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83626	WGS84	BB74203	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB74203_Upin_Bot2011_COI	S2-10	1	BB74203_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74203	Sample	BB74203	targeted	Absent	Sample	Upin_Bot2011_COI	S2-10	COI	45	BB74203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.50837
BB74204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78612	WGS84	BB74204	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB74204_Cjap_Sim2018_COI	S2-13	1	BB74204_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74204	Sample	BB74204	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-13	COI	45	BB74204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51493
BB74204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78612	WGS84	BB74204	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB74204_Mgig_Bot2012_COI	S2-13	1	BB74204_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74204	Sample	BB74204	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-13	COI	45	BB74204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.51493
BB74205	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80954	WGS84	BB74205	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB74205_Cmae_Bot2011_COI	S2-19	1	BB74205_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB74205	Sample	BB74205	targeted	Absent	Sample	Cmae_Bot2011_COI	S2-19	COI	45	BB74205	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-19	ATGAACAGTCTATCCTCCTTTAG	138.51877
BB75216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78716	WGS84	BB75216	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75216_Cjap_Sim2018_COI	S2-15	1	BB75216_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75216	Sample	BB75216	targeted	Absent	Sample	Cjap_Sim2018_COI	S2-15	COI	45	BB75216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51476
BB75216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78716	WGS84	BB75216	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S02	5/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75216_Mgig_Bot2012_COI	S2-15	1	BB75216_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75216	Sample	BB75216	targeted	Absent	Sample	Mgig_Bot2012_COI	S2-15	COI	45	BB75216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.51476
BB75217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80872	WGS84	BB75217	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.624	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75217_Cmae_Bot2011_COI	S2-16	1	BB75217_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75217	Sample	BB75217	targeted	Present	Sample	Cmae_Bot2011_COI	S2-16	COI	45	BB75217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-16	ATGAACAGTCTATCCTCCTTTAG	138.51653
BB75219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80944	WGS84	BB75219	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75219_Aamu_Bax2006_COI	S2-18	1	BB75219_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75219	Sample	BB75219	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-18	COI	45	BB75219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-18	GCACAACCGGGATCTTTACTTC	138.51875
BB75219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80944	WGS84	BB75219	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75219_Pcan_Bot2011_IGS	S2-18	1	BB75219_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75219	Sample	BB75219	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-18	IGS	45	BB75219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-18	CGTAATCCTCAGTACTGGCTA	138.51875
BB75219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80944	WGS84	BB75219	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.045	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75219_Sspa_Oph2007_COI	S2-18	1	BB75219_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75219	Sample	BB75219	targeted	Present	Sample	Sspa_Oph2007_COI	S2-18	COI	45	BB75219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-18	GAAGGCCTTCCGATGATC	138.51875
BB75219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80944	WGS84	BB75219	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75219_Upin_Bot2011_COI	S2-18	1	BB75219_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75219	Sample	BB75219	targeted	Absent	Sample	Upin_Bot2011_COI	S2-18	COI	45	BB75219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-18	CTTTAATTACAGCGTTTTTATTGTTGT	138.51875
BB75222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85712	WGS84	BB75222	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75222_Aamu_Bax2006_COI	T1-13	1	BB75222_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75222	Sample	BB75222	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-13	COI	45	BB75222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-13	GCACAACCGGGATCTTTACTTC	147.87617
BB75222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85712	WGS84	BB75222	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75222_Pcan_Bot2011_IGS	T1-13	1	BB75222_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75222	Sample	BB75222	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-13	IGS	45	BB75222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-13	CGTAATCCTCAGTACTGGCTA	147.87617
BB75222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85712	WGS84	BB75222	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75222_Sspa_Oph2007_COI	T1-13	1	BB75222_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75222	Sample	BB75222	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-13	COI	45	BB75222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-13	GAAGGCCTTCCGATGATC	147.87617
BB75222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85712	WGS84	BB75222	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.248	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75222_Upin_Bot2011_COI	T1-13	1	BB75222_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75222	Sample	BB75222	targeted	Present	Sample	Upin_Bot2011_COI	T1-13	COI	45	BB75222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.87617
BB75223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96422	WGS84	BB75223	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	23/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75223_Cjap_Sim2018_COI	T1-06	1	BB75223_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75223	Sample	BB75223	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-06	COI	45	BB75223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51968
BB75223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.96422	WGS84	BB75223	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.632	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	23/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75223_Mgig_Bot2012_COI	T1-06	1	BB75223_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75223	Sample	BB75223	targeted	Present	Sample	Mgig_Bot2012_COI	T1-06	COI	45	BB75223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.51968
BB75226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87458	WGS84	BB75226	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75226_Aamu_Bax2006_COI	T1-15	1	BB75226_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75226	Sample	BB75226	targeted	Absent	Sample	Aamu_Bax2006_COI	T1-15	COI	45	BB75226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-15	GCACAACCGGGATCTTTACTTC	147.33635
BB75226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87458	WGS84	BB75226	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75226_Pcan_Bot2011_IGS	T1-15	1	BB75226_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75226	Sample	BB75226	targeted	Absent	Sample	Pcan_Bot2011_IGS	T1-15	IGS	45	BB75226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-15	CGTAATCCTCAGTACTGGCTA	147.33635
BB75226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87458	WGS84	BB75226	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75226_Sspa_Oph2007_COI	T1-15	1	BB75226_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75226	Sample	BB75226	targeted	Absent	Sample	Sspa_Oph2007_COI	T1-15	COI	45	BB75226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-15	GAAGGCCTTCCGATGATC	147.33635
BB75226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87458	WGS84	BB75226	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75226_Upin_Bot2011_COI	T1-15	1	BB75226_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75226	Sample	BB75226	targeted	Absent	Sample	Upin_Bot2011_COI	T1-15	COI	45	BB75226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.33635
BB75227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87996	WGS84	BB75227	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75227_Cjap_Sim2018_COI	T1-08	1	BB75227_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75227	Sample	BB75227	targeted	Absent	Sample	Cjap_Sim2018_COI	T1-08	COI	45	BB75227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34157
BB75227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87996	WGS84	BB75227	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.731	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T01	25/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75227_Mgig_Bot2012_COI	T1-08	1	BB75227_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75227	Sample	BB75227	targeted	Present	Sample	Mgig_Bot2012_COI	T1-08	COI	45	BB75227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.34157
BB75228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82735	WGS84	BB75228	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.68	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T01	25/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75228_Cmae_Bot2011_COI	T1-16	1	BB75228_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75228	Sample	BB75228	targeted	Present	Sample	Cmae_Bot2011_COI	T1-16	COI	45	BB75228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T1-16	ATGAACAGTCTATCCTCCTTTAG	147.3021
BB75230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87299	WGS84	BB75230	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75230_Cjap_Sim2018_COI	T4-04	1	BB75230_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75230	Sample	BB75230	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-04	COI	45	BB75230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33679
BB75230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87299	WGS84	BB75230	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75230_Mgig_Bot2012_COI	T4-04	1	BB75230_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75230	Sample	BB75230	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-04	COI	45	BB75230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.33679
BB75231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89813	WGS84	BB75231	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.397	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75231_Cmae_Bot2011_COI	T4-06	1	BB75231_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75231	Sample	BB75231	targeted	Present	Sample	Cmae_Bot2011_COI	T4-06	COI	45	BB75231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-06	ATGAACAGTCTATCCTCCTTTAG	147.34208
BB75233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84798	WGS84	BB75233	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75233_Aamu_Bax2006_COI	T4-10	1	BB75233_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75233	Sample	BB75233	targeted	Absent	Sample	Aamu_Bax2006_COI	T4-10	COI	45	BB75233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-10	GCACAACCGGGATCTTTACTTC	147.87403
BB75233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84798	WGS84	BB75233	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75233_Pcan_Bot2011_IGS	T4-10	1	BB75233_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75233	Sample	BB75233	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-10	IGS	45	BB75233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-10	CGTAATCCTCAGTACTGGCTA	147.87403
BB75233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84798	WGS84	BB75233	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75233_Sspa_Oph2007_COI	T4-10	1	BB75233_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75233	Sample	BB75233	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-10	COI	45	BB75233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-10	GAAGGCCTTCCGATGATC	147.87403
BB75233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.84798	WGS84	BB75233	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75233_Upin_Bot2011_COI	T4-10	1	BB75233_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75233	Sample	BB75233	targeted	Absent	Sample	Upin_Bot2011_COI	T4-10	COI	45	BB75233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.87403
BB75234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.85713	WGS84	BB75234	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75234_Cjap_Sim2018_COI	T4-12	1	BB75234_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75234	Sample	BB75234	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-12	COI	45	BB75234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87276
BB75234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.85713	WGS84	BB75234	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75234_Mgig_Bot2012_COI	T4-12	1	BB75234_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75234	Sample	BB75234	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-12	COI	45	BB75234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.87276
BB75235	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.95924	WGS84	BB75235	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.68	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75235_Cmae_Bot2011_COI	T4-14	1	BB75235_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75235	Sample	BB75235	targeted	Present	Sample	Cmae_Bot2011_COI	T4-14	COI	45	BB75235	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-14	ATGAACAGTCTATCCTCCTTTAG	147.52587
BB75237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82524	WGS84	BB75237	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.849	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75237_Aamu_Bax2006_COI	T4-03	1	BB75237_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75237	Sample	BB75237	targeted	Present	Sample	Aamu_Bax2006_COI	T4-03	COI	45	BB75237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-03	GCACAACCGGGATCTTTACTTC	147.30752
BB75237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82524	WGS84	BB75237	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75237_Pcan_Bot2011_IGS	T4-03	1	BB75237_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75237	Sample	BB75237	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-03	IGS	45	BB75237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-03	CGTAATCCTCAGTACTGGCTA	147.30752
BB75237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82524	WGS84	BB75237	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75237_Sspa_Oph2007_COI	T4-03	1	BB75237_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75237	Sample	BB75237	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-03	COI	45	BB75237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-03	GAAGGCCTTCCGATGATC	147.30752
BB75237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.82524	WGS84	BB75237	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75237_Upin_Bot2011_COI	T4-03	1	BB75237_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75237	Sample	BB75237	targeted	Absent	Sample	Upin_Bot2011_COI	T4-03	COI	45	BB75237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.30752
BB75238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.87854	WGS84	BB75238	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75238_Cjap_Sim2018_COI	T4-05	1	BB75238_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75238	Sample	BB75238	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-05	COI	45	BB75238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34183
BB75238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.87854	WGS84	BB75238	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/05/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75238_Mgig_Bot2012_COI	T4-05	1	BB75238_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75238	Sample	BB75238	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-05	COI	45	BB75238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.34183
BB75239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.89951	WGS84	BB75239	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75239_Cmae_Bot2011_COI	T4-07	1	BB75239_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75239	Sample	BB75239	targeted	Absent	Sample	Cmae_Bot2011_COI	T4-07	COI	45	BB75239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-07	ATGAACAGTCTATCCTCCTTTAG	147.33637
BB75240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88548	WGS84	BB75240	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.725	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75240_Aamu_Bax2006_COI	T4-09	1	BB75240_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75240	Sample	BB75240	targeted	Present	Sample	Aamu_Bax2006_COI	T4-09	COI	45	BB75240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-09	GCACAACCGGGATCTTTACTTC	147.33615
BB75240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88548	WGS84	BB75240	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75240_Pcan_Bot2011_IGS	T4-09	1	BB75240_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75240	Sample	BB75240	targeted	Absent	Sample	Pcan_Bot2011_IGS	T4-09	IGS	45	BB75240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-09	CGTAATCCTCAGTACTGGCTA	147.33615
BB75240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88548	WGS84	BB75240	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75240_Sspa_Oph2007_COI	T4-09	1	BB75240_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75240	Sample	BB75240	targeted	Absent	Sample	Sspa_Oph2007_COI	T4-09	COI	45	BB75240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-09	GAAGGCCTTCCGATGATC	147.33615
BB75240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.88548	WGS84	BB75240	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/05/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75240_Upin_Bot2011_COI	T4-09	1	BB75240_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75240	Sample	BB75240	targeted	Absent	Sample	Upin_Bot2011_COI	T4-09	COI	45	BB75240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.33615
BB75241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.8512	WGS84	BB75241	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB75241_Cjap_Sim2018_COI	T4-11	1	BB75241_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75241	Sample	BB75241	targeted	Absent	Sample	Cjap_Sim2018_COI	T4-11	COI	45	BB75241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87357
BB75241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10065	-42.8512	WGS84	BB75241	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T04	9/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB75241_Mgig_Bot2012_COI	T4-11	1	BB75241_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75241	Sample	BB75241	targeted	Absent	Sample	Mgig_Bot2012_COI	T4-11	COI	45	BB75241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.87357
BB75242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12998	-42.96131	WGS84	BB75242	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.519	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T04	9/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB75242_Cmae_Bot2011_COI	T4-13	1	BB75242_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75242	Sample	BB75242	targeted	Present	Sample	Cmae_Bot2011_COI	T4-13	COI	45	BB75242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T4-13	ATGAACAGTCTATCCTCCTTTAG	147.53343
BB75248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80931	WGS84	BB75248	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB75248_Aamu_Bax2006_COI	S2-20	1	BB75248_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75248	Sample	BB75248	targeted	Absent	Sample	Aamu_Bax2006_COI	S2-20	COI	45	BB75248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-20	GCACAACCGGGATCTTTACTTC	138.5177
BB75248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80931	WGS84	BB75248	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB75248_Pcan_Bot2011_IGS	S2-20	1	BB75248_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75248	Sample	BB75248	targeted	Absent	Sample	Pcan_Bot2011_IGS	S2-20	IGS	45	BB75248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-20	CGTAATCCTCAGTACTGGCTA	138.5177
BB75248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80931	WGS84	BB75248	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.63	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB75248_Sspa_Oph2007_COI	S2-20	1	BB75248_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75248	Sample	BB75248	targeted	Present	Sample	Sspa_Oph2007_COI	S2-20	COI	45	BB75248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-20	GAAGGCCTTCCGATGATC	138.5177
BB75248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80931	WGS84	BB75248	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S02	5/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB75248_Upin_Bot2011_COI	S2-20	1	BB75248_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB75248	Sample	BB75248	targeted	Absent	Sample	Upin_Bot2011_COI	S2-20	COI	45	BB75248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S2-20	CTTTAATTACAGCGTTTTTATTGTTGT	138.5177
BB80077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78295	WGS84	BB80077	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80077_Aamu_Bax2006_COI	S4-02	1	BB80077_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80077	Sample	BB80077	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-02	COI	45	BB80077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-02	GCACAACCGGGATCTTTACTTC	138.51697
BB80077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78295	WGS84	BB80077	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80077_Pcan_Bot2011_IGS	S4-02	1	BB80077_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80077	Sample	BB80077	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-02	IGS	45	BB80077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-02	CGTAATCCTCAGTACTGGCTA	138.51697
BB80077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78295	WGS84	BB80077	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80077_Sspa_Oph2007_COI	S4-02	1	BB80077_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80077	Sample	BB80077	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-02	COI	45	BB80077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-02	GAAGGCCTTCCGATGATC	138.51697
BB80077	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78295	WGS84	BB80077	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80077_Upin_Bot2011_COI	S4-02	1	BB80077_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80077	Sample	BB80077	targeted	Absent	Sample	Upin_Bot2011_COI	S4-02	COI	45	BB80077	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.51697
BB80078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78422	WGS84	BB80078	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80078_Cjap_Sim2018_COI	S4-03	1	BB80078_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80078	Sample	BB80078	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-03	COI	45	BB80078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51657
BB80078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78422	WGS84	BB80078	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.093	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80078_Mgig_Bot2012_COI	S4-03	1	BB80078_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80078	Sample	BB80078	targeted	Present	Sample	Mgig_Bot2012_COI	S4-03	COI	45	BB80078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.51657
BB80081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84367	WGS84	BB80081	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80081_Cjap_Sim2018_COI	S4-06	1	BB80081_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80081	Sample	BB80081	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-06	COI	45	BB80081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49764
BB80081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84367	WGS84	BB80081	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.692	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80081_Mgig_Bot2012_COI	S4-06	1	BB80081_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80081	Sample	BB80081	targeted	Present	Sample	Mgig_Bot2012_COI	S4-06	COI	45	BB80081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.49764
BB80082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84439	WGS84	BB80082	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.434	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80082_Cmae_Bot2011_COI	S4-07	1	BB80082_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80082	Sample	BB80082	targeted	Present	Sample	Cmae_Bot2011_COI	S4-07	COI	45	BB80082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-07	ATGAACAGTCTATCCTCCTTTAG	138.49743
BB80084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84516	WGS84	BB80084	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80084_Aamu_Bax2006_COI	S4-08	1	BB80084_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80084	Sample	BB80084	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-08	COI	45	BB80084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-08	GCACAACCGGGATCTTTACTTC	138.49722
BB80084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84516	WGS84	BB80084	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80084_Pcan_Bot2011_IGS	S4-08	1	BB80084_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80084	Sample	BB80084	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-08	IGS	45	BB80084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-08	CGTAATCCTCAGTACTGGCTA	138.49722
BB80084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84516	WGS84	BB80084	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.533	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80084_Sspa_Oph2007_COI	S4-08	1	BB80084_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80084	Sample	BB80084	targeted	Present	Sample	Sspa_Oph2007_COI	S4-08	COI	45	BB80084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-08	GAAGGCCTTCCGATGATC	138.49722
BB80084	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84516	WGS84	BB80084	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80084_Upin_Bot2011_COI	S4-08	1	BB80084_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80084	Sample	BB80084	targeted	Absent	Sample	Upin_Bot2011_COI	S4-08	COI	45	BB80084	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.49722
BB80085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84657	WGS84	BB80085	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.835	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80085_Mgig_Bot2012_COI	S4-09	1	BB80085_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80085	Sample	BB80085	targeted	Present	Sample	Mgig_Bot2012_COI	S4-09	COI	45	BB80085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.49722
BB80088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8367	WGS84	BB80088	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80088_Aamu_Bax2006_COI	S4-12	1	BB80088_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80088	Sample	BB80088	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-12	COI	45	BB80088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-12	GCACAACCGGGATCTTTACTTC	138.50862
BB80088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8367	WGS84	BB80088	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80088_Pcan_Bot2011_IGS	S4-12	1	BB80088_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80088	Sample	BB80088	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-12	IGS	45	BB80088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-12	CGTAATCCTCAGTACTGGCTA	138.50862
BB80088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8367	WGS84	BB80088	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80088_Sspa_Oph2007_COI	S4-12	1	BB80088_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80088	Sample	BB80088	targeted	Absent	Sample	Sspa_Oph2007_COI	S4-12	COI	45	BB80088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-12	GAAGGCCTTCCGATGATC	138.50862
BB80088	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.8367	WGS84	BB80088	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80088_Upin_Bot2011_COI	S4-12	1	BB80088_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80088	Sample	BB80088	targeted	Absent	Sample	Upin_Bot2011_COI	S4-12	COI	45	BB80088	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.50862
BB80089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83697	WGS84	BB80089	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80089_Cjap_Sim2018_COI	S4-13	1	BB80089_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80089	Sample	BB80089	targeted	Absent	Sample	Cjap_Sim2018_COI	S4-13	COI	45	BB80089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50959
BB80089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83697	WGS84	BB80089	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.417	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80089_Mgig_Bot2012_COI	S4-13	1	BB80089_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80089	Sample	BB80089	targeted	Present	Sample	Mgig_Bot2012_COI	S4-13	COI	45	BB80089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.50959
BB80107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78497	WGS84	BB80107	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80107_Aamu_Bax2006_COI	S3-19	1	BB80107_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80107	Sample	BB80107	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-19	COI	45	BB80107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-19	GCACAACCGGGATCTTTACTTC	138.51619
BB80107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78497	WGS84	BB80107	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80107_Pcan_Bot2011_IGS	S3-19	1	BB80107_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80107	Sample	BB80107	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-19	IGS	45	BB80107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-19	CGTAATCCTCAGTACTGGCTA	138.51619
BB80107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78497	WGS84	BB80107	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80107_Sspa_Oph2007_COI	S3-19	1	BB80107_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80107	Sample	BB80107	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-19	COI	45	BB80107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-19	GAAGGCCTTCCGATGATC	138.51619
BB80107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78497	WGS84	BB80107	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80107_Upin_Bot2011_COI	S3-19	1	BB80107_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80107	Sample	BB80107	targeted	Absent	Sample	Upin_Bot2011_COI	S3-19	COI	45	BB80107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.51619
BB80108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78601	WGS84	BB80108	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80108_Cjap_Sim2018_COI	S3-18	1	BB80108_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80108	Sample	BB80108	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-18	COI	45	BB80108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51598
BB80108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.78601	WGS84	BB80108	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80108_Mgig_Bot2012_COI	S3-18	1	BB80108_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80108	Sample	BB80108	targeted	Absent	Sample	Mgig_Bot2012_COI	S3-18	COI	45	BB80108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.51598
BB80109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78732	WGS84	BB80109	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80109_Cmae_Bot2011_COI	S3-17	1	BB80109_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80109	Sample	BB80109	targeted	Absent	Sample	Cmae_Bot2011_COI	S3-17	COI	45	BB80109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-17	ATGAACAGTCTATCCTCCTTTAG	138.51553
BB80110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78837	WGS84	BB80110	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80110_Aamu_Bax2006_COI	S3-16	1	BB80110_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80110	Sample	BB80110	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-16	COI	45	BB80110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-16	GCACAACCGGGATCTTTACTTC	138.51486
BB80110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78837	WGS84	BB80110	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80110_Pcan_Bot2011_IGS	S3-16	1	BB80110_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80110	Sample	BB80110	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-16	IGS	45	BB80110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-16	CGTAATCCTCAGTACTGGCTA	138.51486
BB80110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78837	WGS84	BB80110	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80110_Sspa_Oph2007_COI	S3-16	1	BB80110_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80110	Sample	BB80110	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-16	COI	45	BB80110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-16	GAAGGCCTTCCGATGATC	138.51486
BB80110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.78837	WGS84	BB80110	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80110_Upin_Bot2011_COI	S3-16	1	BB80110_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80110	Sample	BB80110	targeted	Absent	Sample	Upin_Bot2011_COI	S3-16	COI	45	BB80110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.51486
BB80111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81136	WGS84	BB80111	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80111_Cjap_Sim2018_COI	S3-15	1	BB80111_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80111	Sample	BB80111	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-15	COI	45	BB80111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52303
BB80111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.81136	WGS84	BB80111	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.178	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80111_Mgig_Bot2012_COI	S3-15	1	BB80111_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80111	Sample	BB80111	targeted	Present	Sample	Mgig_Bot2012_COI	S3-15	COI	45	BB80111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.52303
BB80112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.81082	WGS84	BB80112	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.226	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80112_Cmae_Bot2011_COI	S3-14	1	BB80112_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80112	Sample	BB80112	targeted	Present	Sample	Cmae_Bot2011_COI	S3-14	COI	45	BB80112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-14	ATGAACAGTCTATCCTCCTTTAG	138.52156
BB80114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80965	WGS84	BB80114	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80114_Aamu_Bax2006_COI	S3-12	1	BB80114_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80114	Sample	BB80114	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-12	COI	45	BB80114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-12	GCACAACCGGGATCTTTACTTC	138.51882
BB80114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80965	WGS84	BB80114	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80114_Pcan_Bot2011_IGS	S3-12	1	BB80114_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80114	Sample	BB80114	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-12	IGS	45	BB80114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-12	CGTAATCCTCAGTACTGGCTA	138.51882
BB80114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80965	WGS84	BB80114	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.821	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80114_Sspa_Oph2007_COI	S3-12	1	BB80114_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80114	Sample	BB80114	targeted	Present	Sample	Sspa_Oph2007_COI	S3-12	COI	45	BB80114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-12	GAAGGCCTTCCGATGATC	138.51882
BB80114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80965	WGS84	BB80114	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80114_Upin_Bot2011_COI	S3-12	1	BB80114_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80114	Sample	BB80114	targeted	Absent	Sample	Upin_Bot2011_COI	S3-12	COI	45	BB80114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.51882
BB80115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.80903	WGS84	BB80115	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80115_Cjap_Sim2018_COI	S3-11	1	BB80115_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80115	Sample	BB80115	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-11	COI	45	BB80115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51747
BB80115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.80903	WGS84	BB80115	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.018	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80115_Mgig_Bot2012_COI	S3-11	1	BB80115_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80115	Sample	BB80115	targeted	Present	Sample	Mgig_Bot2012_COI	S3-11	COI	45	BB80115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51747
BB80116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83674	WGS84	BB80116	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.423	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80116_Cmae_Bot2011_COI	S3-08	1	BB80116_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80116	Sample	BB80116	targeted	Present	Sample	Cmae_Bot2011_COI	S3-08	COI	45	BB80116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-08	ATGAACAGTCTATCCTCCTTTAG	138.50858
BB80118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83573	WGS84	BB80118	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80118_Aamu_Bax2006_COI	S3-10	1	BB80118_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80118	Sample	BB80118	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-10	COI	45	BB80118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-10	GCACAACCGGGATCTTTACTTC	138.50796
BB80118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83573	WGS84	BB80118	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80118_Pcan_Bot2011_IGS	S3-10	1	BB80118_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80118	Sample	BB80118	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-10	IGS	45	BB80118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-10	CGTAATCCTCAGTACTGGCTA	138.50796
BB80118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83573	WGS84	BB80118	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80118_Sspa_Oph2007_COI	S3-10	1	BB80118_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80118	Sample	BB80118	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-10	COI	45	BB80118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-10	GAAGGCCTTCCGATGATC	138.50796
BB80118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83573	WGS84	BB80118	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80118_Upin_Bot2011_COI	S3-10	1	BB80118_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80118	Sample	BB80118	targeted	Absent	Sample	Upin_Bot2011_COI	S3-10	COI	45	BB80118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.50796
BB80119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.83679	WGS84	BB80119	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80119_Cjap_Sim2018_COI	S3-07	1	BB80119_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80119	Sample	BB80119	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-07	COI	45	BB80119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50733
BB80119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.83679	WGS84	BB80119	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80119_Mgig_Bot2012_COI	S3-07	1	BB80119_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80119	Sample	BB80119	targeted	Present	Sample	Mgig_Bot2012_COI	S3-07	COI	45	BB80119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.50733
BB80121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84305	WGS84	BB80121	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80121_Aamu_Bax2006_COI	S3-05	1	BB80121_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80121	Sample	BB80121	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-05	COI	45	BB80121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-05	GCACAACCGGGATCTTTACTTC	138.49947
BB80121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84305	WGS84	BB80121	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80121_Pcan_Bot2011_IGS	S3-05	1	BB80121_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80121	Sample	BB80121	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-05	IGS	45	BB80121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-05	CGTAATCCTCAGTACTGGCTA	138.49947
BB80121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84305	WGS84	BB80121	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80121_Sspa_Oph2007_COI	S3-05	1	BB80121_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80121	Sample	BB80121	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-05	COI	45	BB80121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-05	GAAGGCCTTCCGATGATC	138.49947
BB80121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84305	WGS84	BB80121	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80121_Upin_Bot2011_COI	S3-05	1	BB80121_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80121	Sample	BB80121	targeted	Absent	Sample	Upin_Bot2011_COI	S3-05	COI	45	BB80121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-05	CTTTAATTACAGCGTTTTTATTGTTGT	138.49947
BB80122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84355	WGS84	BB80122	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB80122_Cjap_Sim2018_COI	S3-04	1	BB80122_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80122	Sample	BB80122	targeted	Absent	Sample	Cjap_Sim2018_COI	S3-04	COI	45	BB80122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49824
BB80122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10119	-34.84355	WGS84	BB80122	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.345	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S03	14/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80122_Mgig_Bot2012_COI	S3-04	1	BB80122_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80122	Sample	BB80122	targeted	Present	Sample	Mgig_Bot2012_COI	S3-04	COI	45	BB80122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49824
BB80123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84423	WGS84	BB80123	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	26.607	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80123_Cmae_Bot2011_COI	S3-03	1	BB80123_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80123	Sample	BB80123	targeted	Present	Sample	Cmae_Bot2011_COI	S3-03	COI	45	BB80123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-03	ATGAACAGTCTATCCTCCTTTAG	138.49776
BB80125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84665	WGS84	BB80125	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80125_Aamu_Bax2006_COI	S3-01	1	BB80125_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80125	Sample	BB80125	targeted	Absent	Sample	Aamu_Bax2006_COI	S3-01	COI	45	BB80125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-01	GCACAACCGGGATCTTTACTTC	138.49724
BB80125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84665	WGS84	BB80125	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80125_Pcan_Bot2011_IGS	S3-01	1	BB80125_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80125	Sample	BB80125	targeted	Absent	Sample	Pcan_Bot2011_IGS	S3-01	IGS	45	BB80125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-01	CGTAATCCTCAGTACTGGCTA	138.49724
BB80125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84665	WGS84	BB80125	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80125_Sspa_Oph2007_COI	S3-01	1	BB80125_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80125	Sample	BB80125	targeted	Absent	Sample	Sspa_Oph2007_COI	S3-01	COI	45	BB80125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-01	GAAGGCCTTCCGATGATC	138.49724
BB80125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12999	-34.84665	WGS84	BB80125	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S03	14/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80125_Upin_Bot2011_COI	S3-01	1	BB80125_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80125	Sample	BB80125	targeted	Absent	Sample	Upin_Bot2011_COI	S3-01	COI	45	BB80125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S3-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.49724
BB80145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38774	WGS84	BB80145	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80145_Cmae_Bot2011_COI	BB22	1	BB80145_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80145	Sample	BB80145	targeted	Absent	Sample	Cmae_Bot2011_COI	BB22	COI	45	BB80145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB22	ATGAACAGTCTATCCTCCTTTAG	153.15869
BB80145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38774	WGS84	BB80145	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80145_Msal_Bot2012_COI	BB22	1	BB80145_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80145	Sample	BB80145	targeted	Absent	Sample	Msal_Bot2012_COI	BB22	COI	45	BB80145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB22	GYTAGTTCCRATGATGTTAGCTG	153.15869
BB80147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38249	WGS84	BB80147	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80147_Aamu_Bax2006_COI	BB18	1	BB80147_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80147	Sample	BB80147	targeted	Absent	Sample	Aamu_Bax2006_COI	BB18	COI	45	BB80147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB18	GCACAACCGGGATCTTTACTTC	153.16121
BB80147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38249	WGS84	BB80147	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80147_Pcan_Bot2011_IGS	BB18	1	BB80147_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80147	Sample	BB80147	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB18	IGS	45	BB80147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB18	CGTAATCCTCAGTACTGGCTA	153.16121
BB80147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38249	WGS84	BB80147	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80147_Sspa_Oph2007_COI	BB18	1	BB80147_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80147	Sample	BB80147	targeted	Absent	Sample	Sspa_Oph2007_COI	BB18	COI	45	BB80147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB18	GAAGGCCTTCCGATGATC	153.16121
BB80148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38	WGS84	BB80148	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80148_Mgig_Bot2012_COI	BB16	1	BB80148_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80148	Sample	BB80148	targeted	Absent	Sample	Mgig_Bot2012_COI	BB16	COI	45	BB80148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB16	TCTTATTCGTTGGAGACTTTATAACCCT	153.16327
BB80149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.376	WGS84	BB80149	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80149_Cmae_Bot2011_COI	BB13	1	BB80149_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80149	Sample	BB80149	targeted	Absent	Sample	Cmae_Bot2011_COI	BB13	COI	45	BB80149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB13	ATGAACAGTCTATCCTCCTTTAG	153.16704
BB80149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.376	WGS84	BB80149	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80149_Msal_Bot2012_COI	BB13	1	BB80149_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80149	Sample	BB80149	targeted	Absent	Sample	Msal_Bot2012_COI	BB13	COI	45	BB80149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB13	GYTAGTTCCRATGATGTTAGCTG	153.16704
BB80150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37228	WGS84	BB80150	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80150_Aamu_Bax2006_COI	BB11	1	BB80150_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80150	Sample	BB80150	targeted	Absent	Sample	Aamu_Bax2006_COI	BB11	COI	45	BB80150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB11	GCACAACCGGGATCTTTACTTC	153.16969
BB80150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37228	WGS84	BB80150	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80150_Pcan_Bot2011_IGS	BB11	1	BB80150_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80150	Sample	BB80150	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB11	IGS	45	BB80150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB11	CGTAATCCTCAGTACTGGCTA	153.16969
BB80150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37228	WGS84	BB80150	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80150_Sspa_Oph2007_COI	BB11	1	BB80150_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80150	Sample	BB80150	targeted	Absent	Sample	Sspa_Oph2007_COI	BB11	COI	45	BB80150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB11	GAAGGCCTTCCGATGATC	153.16969
BB80150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37228	WGS84	BB80150	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80150_Upin_Bot2011_COI	BB11	1	BB80150_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80150	Sample	BB80150	targeted	Absent	Sample	Upin_Bot2011_COI	BB11	COI	45	BB80150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB11	CTTTAATTACAGCGTTTTTATTGTTGT	153.16969
BB80151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.35698	WGS84	BB80151	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80151_Mgig_Bot2012_COI	BB1	1	BB80151_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80151	Sample	BB80151	targeted	Absent	Sample	Mgig_Bot2012_COI	BB1	COI	45	BB80151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB1	TCTTATTCGTTGGAGACTTTATAACCCT	153.18056
BB80152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44756	WGS84	BB80152	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80152_Cmae_Bot2011_COI	BB64	1	BB80152_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80152	Sample	BB80152	targeted	Absent	Sample	Cmae_Bot2011_COI	BB64	COI	45	BB80152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB64	ATGAACAGTCTATCCTCCTTTAG	153.09609
BB80152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44756	WGS84	BB80152	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80152_Msal_Bot2012_COI	BB64	1	BB80152_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80152	Sample	BB80152	targeted	Absent	Sample	Msal_Bot2012_COI	BB64	COI	45	BB80152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB64	GYTAGTTCCRATGATGTTAGCTG	153.09609
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80154_Aamu_Bax2006_COI	MB90	1	BB80154_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Absent	Sample	Aamu_Bax2006_COI	MB90	COI	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	GCACAACCGGGATCTTTACTTC	144.91468
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80154_Pcan_Bot2011_IGS	MB90	1	BB80154_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB90	IGS	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	CGTAATCCTCAGTACTGGCTA	144.91468
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.08489609	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80154_Sspa_Oph2007_COI	MB90	1	BB80154_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Present	Sample	Sspa_Oph2007_COI	MB90	COI	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	GAAGGCCTTCCGATGATC	144.91468
BB80154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81663	WGS84	BB80154	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	41.614	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80154_Upin_Bot2011_COI	MB90	1	BB80154_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80154	Sample	BB80154	targeted	Present	Sample	Upin_Bot2011_COI	MB90	COI	45	BB80154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB90	CTTTAATTACAGCGTTTTTATTGTTGT	144.91468
BB80155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81722	WGS84	BB80155	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80155_Mgig_Bot2012_COI	MB85	1	BB80155_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80155	Sample	BB80155	targeted	Absent	Sample	Mgig_Bot2012_COI	MB85	COI	45	BB80155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB85	TCTTATTCGTTGGAGACTTTATAACCCT	144.92112
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80156_Cmae_Bot2011_COI	MB80	1	BB80156_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Absent	Sample	Cmae_Bot2011_COI	MB80	COI	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	ATGAACAGTCTATCCTCCTTTAG	144.92768
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80156_Hsan_Wil2021_COI	MB80	1	BB80156_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Absent	Sample	Hsan_Wil2021_COI	MB80	COI	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	CCTGGGCCGGTATAGTAGGT	144.92768
BB80156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81586	WGS84	BB80156	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80156_Msal_Bot2012_COI	MB80	1	BB80156_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80156	Sample	BB80156	targeted	Absent	Sample	Msal_Bot2012_COI	MB80	COI	45	BB80156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB80	GYTAGTTCCRATGATGTTAGCTG	144.92768
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80158_Aamu_Bax2006_COI	MB60	1	BB80158_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Absent	Sample	Aamu_Bax2006_COI	MB60	COI	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	GCACAACCGGGATCTTTACTTC	144.90884
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80158_Pcan_Bot2011_IGS	MB60	1	BB80158_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB60	IGS	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	CGTAATCCTCAGTACTGGCTA	144.90884
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.01603699	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80158_Sspa_Oph2007_COI	MB60	1	BB80158_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Present	Sample	Sspa_Oph2007_COI	MB60	COI	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	GAAGGCCTTCCGATGATC	144.90884
BB80158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82233	WGS84	BB80158	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80158_Upin_Bot2011_COI	MB60	1	BB80158_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80158	Sample	BB80158	targeted	Absent	Sample	Upin_Bot2011_COI	MB60	COI	45	BB80158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB60	CTTTAATTACAGCGTTTTTATTGTTGT	144.90884
BB80159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81868	WGS84	BB80159	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.562	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80159_Mgig_Bot2012_COI	MB105	1	BB80159_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80159	Sample	BB80159	targeted	Present	Sample	Mgig_Bot2012_COI	MB105	COI	45	BB80159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB105	TCTTATTCGTTGGAGACTTTATAACCCT	144.91163
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.535	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80161_Aamu_Bax2006_COI	MB115	1	BB80161_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Present	Sample	Aamu_Bax2006_COI	MB115	COI	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	GCACAACCGGGATCTTTACTTC	144.90568
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80161_Pcan_Bot2011_IGS	MB115	1	BB80161_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB115	IGS	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	CGTAATCCTCAGTACTGGCTA	144.90568
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.42890167	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80161_Sspa_Oph2007_COI	MB115	1	BB80161_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Present	Sample	Sspa_Oph2007_COI	MB115	COI	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	GAAGGCCTTCCGATGATC	144.90568
BB80161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81622	WGS84	BB80161	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80161_Upin_Bot2011_COI	MB115	1	BB80161_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80161	Sample	BB80161	targeted	Absent	Sample	Upin_Bot2011_COI	MB115	COI	45	BB80161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB115	CTTTAATTACAGCGTTTTTATTGTTGT	144.90568
BB80162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81969	WGS84	BB80162	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.517	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80162_Mgig_Bot2012_COI	MB110	1	BB80162_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80162	Sample	BB80162	targeted	Present	Sample	Mgig_Bot2012_COI	MB110	COI	45	BB80162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB110	TCTTATTCGTTGGAGACTTTATAACCCT	144.90763
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.621	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80163_Cmae_Bot2011_COI	MB50	1	BB80163_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Present	Sample	Cmae_Bot2011_COI	MB50	COI	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	ATGAACAGTCTATCCTCCTTTAG	144.90737
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.573	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80163_Hsan_Wil2021_COI	MB50	1	BB80163_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Present	Sample	Hsan_Wil2021_COI	MB50	COI	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	CCTGGGCCGGTATAGTAGGT	144.90737
BB80163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.8615	WGS84	BB80163	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80163_Msal_Bot2012_COI	MB50	1	BB80163_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80163	Sample	BB80163	targeted	Absent	Sample	Msal_Bot2012_COI	MB50	COI	45	BB80163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB50	GYTAGTTCCRATGATGTTAGCTG	144.90737
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.259	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80165_Aamu_Bax2006_COI	MB40	1	BB80165_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Present	Sample	Aamu_Bax2006_COI	MB40	COI	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	GCACAACCGGGATCTTTACTTC	144.9135
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80165_Pcan_Bot2011_IGS	MB40	1	BB80165_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB40	IGS	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	CGTAATCCTCAGTACTGGCTA	144.9135
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.34200668	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80165_Sspa_Oph2007_COI	MB40	1	BB80165_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Present	Sample	Sspa_Oph2007_COI	MB40	COI	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	GAAGGCCTTCCGATGATC	144.9135
BB80165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86365	WGS84	BB80165	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.204	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80165_Upin_Bot2011_COI	MB40	1	BB80165_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80165	Sample	BB80165	targeted	Present	Sample	Upin_Bot2011_COI	MB40	COI	45	BB80165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB40	CTTTAATTACAGCGTTTTTATTGTTGT	144.9135
BB80166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86578	WGS84	BB80166	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80166_Mgig_Bot2012_COI	MB35	1	BB80166_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80166	Sample	BB80166	targeted	Absent	Sample	Mgig_Bot2012_COI	MB35	COI	45	BB80166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB35	TCTTATTCGTTGGAGACTTTATAACCCT	144.91773
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80167_Cmae_Bot2011_COI	MB30	1	BB80167_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Absent	Sample	Cmae_Bot2011_COI	MB30	COI	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	ATGAACAGTCTATCCTCCTTTAG	144.91604
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80167_Hsan_Wil2021_COI	MB30	1	BB80167_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Absent	Sample	Hsan_Wil2021_COI	MB30	COI	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	CCTGGGCCGGTATAGTAGGT	144.91604
BB80167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86339	WGS84	BB80167	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80167_Msal_Bot2012_COI	MB30	1	BB80167_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80167	Sample	BB80167	targeted	Absent	Sample	Msal_Bot2012_COI	MB30	COI	45	BB80167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB30	GYTAGTTCCRATGATGTTAGCTG	144.91604
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.019	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80169_Aamu_Bax2006_COI	MB15	1	BB80169_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Present	Sample	Aamu_Bax2006_COI	MB15	COI	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	GCACAACCGGGATCTTTACTTC	144.90877
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80169_Pcan_Bot2011_IGS	MB15	1	BB80169_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB15	IGS	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	CGTAATCCTCAGTACTGGCTA	144.90877
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.41094589	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80169_Sspa_Oph2007_COI	MB15	1	BB80169_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Present	Sample	Sspa_Oph2007_COI	MB15	COI	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	GAAGGCCTTCCGATGATC	144.90877
BB80169	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84967	WGS84	BB80169	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.652	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80169_Upin_Bot2011_COI	MB15	1	BB80169_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80169	Sample	BB80169	targeted	Present	Sample	Upin_Bot2011_COI	MB15	COI	45	BB80169	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB15	CTTTAATTACAGCGTTTTTATTGTTGT	144.90877
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80170_Cmae_Bot2011_COI	MB10	1	BB80170_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Absent	Sample	Cmae_Bot2011_COI	MB10	COI	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	ATGAACAGTCTATCCTCCTTTAG	144.90737
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80170_Hsan_Wil2021_COI	MB10	1	BB80170_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Absent	Sample	Hsan_Wil2021_COI	MB10	COI	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	CCTGGGCCGGTATAGTAGGT	144.90737
BB80170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84398	WGS84	BB80170	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80170_Msal_Bot2012_COI	MB10	1	BB80170_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80170	Sample	BB80170	targeted	Absent	Sample	Msal_Bot2012_COI	MB10	COI	45	BB80170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB10	GYTAGTTCCRATGATGTTAGCTG	144.90737
BB80177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81974	WGS84	BB80177	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.819	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80177_Mgig_Bot2012_COI	MB108	1	BB80177_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80177	Sample	BB80177	targeted	Present	Sample	Mgig_Bot2012_COI	MB108	COI	45	BB80177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB108	TCTTATTCGTTGGAGACTTTATAACCCT	144.90959
BB80178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81639	WGS84	BB80178	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80178_Cmae_Bot2011_COI	MB104	1	BB80178_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80178	Sample	BB80178	targeted	Absent	Sample	Cmae_Bot2011_COI	MB104	COI	45	BB80178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB104	ATGAACAGTCTATCCTCCTTTAG	144.91286
BB80178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81639	WGS84	BB80178	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80178_Msal_Bot2012_COI	MB104	1	BB80178_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80178	Sample	BB80178	targeted	Absent	Sample	Msal_Bot2012_COI	MB104	COI	45	BB80178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB104	GYTAGTTCCRATGATGTTAGCTG	144.91286
BB80180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81	WGS84	BB80180	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.226	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80180_Mgig_Bot2012_COI	MB98	1	BB80180_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80180	Sample	BB80180	targeted	Present	Sample	Mgig_Bot2012_COI	MB98	COI	45	BB80180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB98	TCTTATTCGTTGGAGACTTTATAACCCT	144.91304
BB80181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.80956	WGS84	BB80181	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80181_Cmae_Bot2011_COI	MB96	1	BB80181_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80181	Sample	BB80181	targeted	Absent	Sample	Cmae_Bot2011_COI	MB96	COI	45	BB80181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB96	ATGAACAGTCTATCCTCCTTTAG	144.91452
BB80181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.80956	WGS84	BB80181	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80181_Msal_Bot2012_COI	MB96	1	BB80181_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80181	Sample	BB80181	targeted	Absent	Sample	Msal_Bot2012_COI	MB96	COI	45	BB80181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB96	GYTAGTTCCRATGATGTTAGCTG	144.91452
BB80183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81479	WGS84	BB80183	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.972	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80183_Aamu_Bax2006_COI	MB92	1	BB80183_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80183	Sample	BB80183	targeted	Present	Sample	Aamu_Bax2006_COI	MB92	COI	45	BB80183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB92	GCACAACCGGGATCTTTACTTC	144.91433
BB80183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81479	WGS84	BB80183	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80183_Pcan_Bot2011_IGS	MB92	1	BB80183_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80183	Sample	BB80183	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB92	IGS	45	BB80183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB92	CGTAATCCTCAGTACTGGCTA	144.91433
BB80183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81479	WGS84	BB80183	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.29427719	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80183_Sspa_Oph2007_COI	MB92	1	BB80183_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80183	Sample	BB80183	targeted	Present	Sample	Sspa_Oph2007_COI	MB92	COI	45	BB80183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB92	GAAGGCCTTCCGATGATC	144.91433
BB80183	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81479	WGS84	BB80183	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80183_Upin_Bot2011_COI	MB92	1	BB80183_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80183	Sample	BB80183	targeted	Absent	Sample	Upin_Bot2011_COI	MB92	COI	45	BB80183	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB92	CTTTAATTACAGCGTTTTTATTGTTGT	144.91433
BB80184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81828	WGS84	BB80184	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.642	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80184_Mgig_Bot2012_COI	MB89	1	BB80184_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80184	Sample	BB80184	targeted	Present	Sample	Mgig_Bot2012_COI	MB89	COI	45	BB80184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB89	TCTTATTCGTTGGAGACTTTATAACCCT	144.91499
BB80185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81841	WGS84	BB80185	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80185_Cmae_Bot2011_COI	MB87	1	BB80185_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80185	Sample	BB80185	targeted	Absent	Sample	Cmae_Bot2011_COI	MB87	COI	45	BB80185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB87	ATGAACAGTCTATCCTCCTTTAG	144.91788
BB80185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81841	WGS84	BB80185	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80185_Msal_Bot2012_COI	MB87	1	BB80185_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80185	Sample	BB80185	targeted	Absent	Sample	Msal_Bot2012_COI	MB87	COI	45	BB80185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB87	GYTAGTTCCRATGATGTTAGCTG	144.91788
BB80187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81514	WGS84	BB80187	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80187_Aamu_Bax2006_COI	MB81	1	BB80187_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80187	Sample	BB80187	targeted	Absent	Sample	Aamu_Bax2006_COI	MB81	COI	45	BB80187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB81	GCACAACCGGGATCTTTACTTC	144.92658
BB80187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81514	WGS84	BB80187	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80187_Pcan_Bot2011_IGS	MB81	1	BB80187_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80187	Sample	BB80187	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB81	IGS	45	BB80187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB81	CGTAATCCTCAGTACTGGCTA	144.92658
BB80187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81514	WGS84	BB80187	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.70307922	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80187_Sspa_Oph2007_COI	MB81	1	BB80187_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80187	Sample	BB80187	targeted	Present	Sample	Sspa_Oph2007_COI	MB81	COI	45	BB80187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB81	GAAGGCCTTCCGATGATC	144.92658
BB80187	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81514	WGS84	BB80187	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80187_Upin_Bot2011_COI	MB81	1	BB80187_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80187	Sample	BB80187	targeted	Absent	Sample	Upin_Bot2011_COI	MB81	COI	45	BB80187	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB81	CTTTAATTACAGCGTTTTTATTGTTGT	144.92658
BB80188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81667	WGS84	BB80188	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80188_Mgig_Bot2012_COI	MB78	1	BB80188_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80188	Sample	BB80188	targeted	Absent	Sample	Mgig_Bot2012_COI	MB78	COI	45	BB80188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB78	TCTTATTCGTTGGAGACTTTATAACCCT	144.92656
BB80189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81818	WGS84	BB80189	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80189_Cmae_Bot2011_COI	MB76	1	BB80189_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80189	Sample	BB80189	targeted	Absent	Sample	Cmae_Bot2011_COI	MB76	COI	45	BB80189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB76	ATGAACAGTCTATCCTCCTTTAG	144.92699
BB80189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81818	WGS84	BB80189	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80189_Msal_Bot2012_COI	MB76	1	BB80189_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80189	Sample	BB80189	targeted	Absent	Sample	Msal_Bot2012_COI	MB76	COI	45	BB80189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB76	GYTAGTTCCRATGATGTTAGCTG	144.92699
BB80190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81812	WGS84	BB80190	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80190_Aamu_Bax2006_COI	MB73	1	BB80190_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80190	Sample	BB80190	targeted	Absent	Sample	Aamu_Bax2006_COI	MB73	COI	45	BB80190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB73	GCACAACCGGGATCTTTACTTC	144.93004
BB80190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81812	WGS84	BB80190	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80190_Pcan_Bot2011_IGS	MB73	1	BB80190_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80190	Sample	BB80190	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB73	IGS	45	BB80190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB73	CGTAATCCTCAGTACTGGCTA	144.93004
BB80190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81812	WGS84	BB80190	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80190_Sspa_Oph2007_COI	MB73	1	BB80190_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80190	Sample	BB80190	targeted	Absent	Sample	Sspa_Oph2007_COI	MB73	COI	45	BB80190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB73	GAAGGCCTTCCGATGATC	144.93004
BB80190	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81812	WGS84	BB80190	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80190_Upin_Bot2011_COI	MB73	1	BB80190_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80190	Sample	BB80190	targeted	Absent	Sample	Upin_Bot2011_COI	MB73	COI	45	BB80190	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB73	CTTTAATTACAGCGTTTTTATTGTTGT	144.93004
BB80191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81807	WGS84	BB80191	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80191_Mgig_Bot2012_COI	MB72	1	BB80191_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80191	Sample	BB80191	targeted	Absent	Sample	Mgig_Bot2012_COI	MB72	COI	45	BB80191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB72	TCTTATTCGTTGGAGACTTTATAACCCT	144.93189
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81889	WGS84	BB80192	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80192_Cmae_Bot2011_COI	MB71	1	BB80192_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Absent	Sample	Cmae_Bot2011_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	ATGAACAGTCTATCCTCCTTTAG	144.93166
BB80192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81889	WGS84	BB80192	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80192_Msal_Bot2012_COI	MB71	1	BB80192_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80192	Sample	BB80192	targeted	Absent	Sample	Msal_Bot2012_COI	MB71	COI	45	BB80192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB71	GYTAGTTCCRATGATGTTAGCTG	144.93166
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82022	WGS84	BB80194	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80194_Aamu_Bax2006_COI	MB67	1	BB80194_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Aamu_Bax2006_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	GCACAACCGGGATCTTTACTTC	144.92791
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82022	WGS84	BB80194	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80194_Pcan_Bot2011_IGS	MB67	1	BB80194_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB67	IGS	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	CGTAATCCTCAGTACTGGCTA	144.92791
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82022	WGS84	BB80194	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80194_Sspa_Oph2007_COI	MB67	1	BB80194_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Sspa_Oph2007_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	GAAGGCCTTCCGATGATC	144.92791
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82022	WGS84	BB80194	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80194_Upin_Bot2011_COI	MB67	1	BB80194_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Upin_Bot2011_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	CTTTAATTACAGCGTTTTTATTGTTGT	144.92791
BB80194	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82022	WGS84	BB80194	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80194_Vgib_Wil2025_COI	MB67	1	BB80194_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80194	Sample	BB80194	targeted	Absent	Sample	Vgib_Wil2025_COI	MB67	COI	45	BB80194	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB67	CCCCTCTTTACAAGTTCTTTAGGGT	144.92791
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81776	WGS84	BB80195	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	37.286	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80195_Asen_Wil2025_COI	MB77	1	BB80195_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Present	Sample	Asen_Wil2025_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	GTTGTTGTTACAACACATGCTCTTTTG	144.92569
BB80195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81776	WGS84	BB80195	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80195_Mgig_Bot2012_COI	MB77	1	BB80195_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80195	Sample	BB80195	targeted	Absent	Sample	Mgig_Bot2012_COI	MB77	COI	45	BB80195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB77	TCTTATTCGTTGGAGACTTTATAACCCT	144.92569
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81986	WGS84	BB80196	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80196_Cmae_Bot2011_COI	MB66	1	BB80196_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Cmae_Bot2011_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	ATGAACAGTCTATCCTCCTTTAG	144.9239
BB80196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81986	WGS84	BB80196	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80196_Msal_Bot2012_COI	MB66	1	BB80196_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80196	Sample	BB80196	targeted	Absent	Sample	Msal_Bot2012_COI	MB66	COI	45	BB80196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB66	GYTAGTTCCRATGATGTTAGCTG	144.9239
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82068	WGS84	BB80198	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80198_Aamu_Bax2006_COI	MB62	1	BB80198_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Absent	Sample	Aamu_Bax2006_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	GCACAACCGGGATCTTTACTTC	144.91443
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82068	WGS84	BB80198	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80198_Pcan_Bot2011_IGS	MB62	1	BB80198_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB62	IGS	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	CGTAATCCTCAGTACTGGCTA	144.91443
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82068	WGS84	BB80198	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.38518524	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80198_Sspa_Oph2007_COI	MB62	1	BB80198_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Present	Sample	Sspa_Oph2007_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	GAAGGCCTTCCGATGATC	144.91443
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82068	WGS84	BB80198	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80198_Upin_Bot2011_COI	MB62	1	BB80198_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Absent	Sample	Upin_Bot2011_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	CTTTAATTACAGCGTTTTTATTGTTGT	144.91443
BB80198	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82068	WGS84	BB80198	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80198_Vgib_Wil2025_COI	MB62	1	BB80198_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80198	Sample	BB80198	targeted	Absent	Sample	Vgib_Wil2025_COI	MB62	COI	45	BB80198	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB62	CCCCTCTTTACAAGTTCTTTAGGGT	144.91443
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82144	WGS84	BB80199	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.5	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80199_Asen_Wil2025_COI	MB61	1	BB80199_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Present	Sample	Asen_Wil2025_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	GTTGTTGTTACAACACATGCTCTTTTG	144.91152
BB80199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82144	WGS84	BB80199	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80199_Mgig_Bot2012_COI	MB61	1	BB80199_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80199	Sample	BB80199	targeted	Absent	Sample	Mgig_Bot2012_COI	MB61	COI	45	BB80199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB61	TCTTATTCGTTGGAGACTTTATAACCCT	144.91152
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82492	WGS84	BB80200	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80200_Cmae_Bot2011_COI	MB58	1	BB80200_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Absent	Sample	Cmae_Bot2011_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	ATGAACAGTCTATCCTCCTTTAG	144.90413
BB80200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82492	WGS84	BB80200	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80200_Msal_Bot2012_COI	MB58	1	BB80200_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80200	Sample	BB80200	targeted	Absent	Sample	Msal_Bot2012_COI	MB58	COI	45	BB80200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB58	GYTAGTTCCRATGATGTTAGCTG	144.90413
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82694	WGS84	BB80202	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.393	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80202_Aamu_Bax2006_COI	MB126	1	BB80202_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Present	Sample	Aamu_Bax2006_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	GCACAACCGGGATCTTTACTTC	144.89947
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82694	WGS84	BB80202	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80202_Pcan_Bot2011_IGS	MB126	1	BB80202_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB126	IGS	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	CGTAATCCTCAGTACTGGCTA	144.89947
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82694	WGS84	BB80202	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.94984055	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80202_Sspa_Oph2007_COI	MB126	1	BB80202_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Present	Sample	Sspa_Oph2007_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	GAAGGCCTTCCGATGATC	144.89947
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82694	WGS84	BB80202	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80202_Upin_Bot2011_COI	MB126	1	BB80202_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Absent	Sample	Upin_Bot2011_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	CTTTAATTACAGCGTTTTTATTGTTGT	144.89947
BB80202	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.82694	WGS84	BB80202	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80202_Vgib_Wil2025_COI	MB126	1	BB80202_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80202	Sample	BB80202	targeted	Absent	Sample	Vgib_Wil2025_COI	MB126	COI	45	BB80202	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB126	CCCCTCTTTACAAGTTCTTTAGGGT	144.89947
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.825	WGS84	BB80203	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.149	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80203_Asen_Wil2025_COI	MB124	1	BB80203_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Present	Sample	Asen_Wil2025_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	GTTGTTGTTACAACACATGCTCTTTTG	144.90061
BB80203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.825	WGS84	BB80203	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.438	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80203_Mgig_Bot2012_COI	MB124	1	BB80203_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80203	Sample	BB80203	targeted	Present	Sample	Mgig_Bot2012_COI	MB124	COI	45	BB80203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB124	TCTTATTCGTTGGAGACTTTATAACCCT	144.90061
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82329	WGS84	BB80204	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80204_Cmae_Bot2011_COI	MB121	1	BB80204_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Absent	Sample	Cmae_Bot2011_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	ATGAACAGTCTATCCTCCTTTAG	144.90234
BB80204	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.82329	WGS84	BB80204	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80204_Msal_Bot2012_COI	MB121	1	BB80204_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80204	Sample	BB80204	targeted	Absent	Sample	Msal_Bot2012_COI	MB121	COI	45	BB80204	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB121	GYTAGTTCCRATGATGTTAGCTG	144.90234
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81765	WGS84	BB80206	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80206_Aamu_Bax2006_COI	MB116	1	BB80206_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Absent	Sample	Aamu_Bax2006_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	GCACAACCGGGATCTTTACTTC	144.9054
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81765	WGS84	BB80206	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80206_Pcan_Bot2011_IGS	MB116	1	BB80206_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB116	IGS	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	CGTAATCCTCAGTACTGGCTA	144.9054
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81765	WGS84	BB80206	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.45166397	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80206_Sspa_Oph2007_COI	MB116	1	BB80206_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Present	Sample	Sspa_Oph2007_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	GAAGGCCTTCCGATGATC	144.9054
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81765	WGS84	BB80206	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80206_Upin_Bot2011_COI	MB116	1	BB80206_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Absent	Sample	Upin_Bot2011_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	CTTTAATTACAGCGTTTTTATTGTTGT	144.9054
BB80206	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81765	WGS84	BB80206	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	37.649	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB80206_Vgib_Wil2025_COI	MB116	1	BB80206_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80206	Sample	BB80206	targeted	Present	Sample	Vgib_Wil2025_COI	MB116	COI	45	BB80206	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB116	CCCCTCTTTACAAGTTCTTTAGGGT	144.9054
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-37.81725	WGS84	BB80207	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	31.057	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB80207_Asen_Wil2025_COI	MB113	1	BB80207_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Present	Sample	Asen_Wil2025_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	GTTGTTGTTACAACACATGCTCTTTTG	144.90634
BB80207	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81725	WGS84	BB80207	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.332	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80207_Mgig_Bot2012_COI	MB113	1	BB80207_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80207	Sample	BB80207	targeted	Present	Sample	Mgig_Bot2012_COI	MB113	COI	45	BB80207	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB113	TCTTATTCGTTGGAGACTTTATAACCCT	144.90634
BB80208	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81853	WGS84	BB80208	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80208_Cmae_Bot2011_COI	MB112	1	BB80208_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80208	Sample	BB80208	targeted	Absent	Sample	Cmae_Bot2011_COI	MB112	COI	45	BB80208	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB112	ATGAACAGTCTATCCTCCTTTAG	144.90678
BB80208	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.81853	WGS84	BB80208	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80208_Msal_Bot2012_COI	MB112	1	BB80208_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80208	Sample	BB80208	targeted	Absent	Sample	Msal_Bot2012_COI	MB112	COI	45	BB80208	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB112	GYTAGTTCCRATGATGTTAGCTG	144.90678
BB80210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.85743	WGS84	BB80210	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80210_Mgig_Bot2012_COI	MB52	1	BB80210_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80210	Sample	BB80210	targeted	Absent	Sample	Mgig_Bot2012_COI	MB52	COI	45	BB80210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB52	TCTTATTCGTTGGAGACTTTATAACCCT	144.90744
BB80211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.861	WGS84	BB80211	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80211_Cmae_Bot2011_COI	MB49	1	BB80211_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80211	Sample	BB80211	targeted	Absent	Sample	Cmae_Bot2011_COI	MB49	COI	45	BB80211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB49	ATGAACAGTCTATCCTCCTTTAG	144.90817
BB80211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.861	WGS84	BB80211	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80211_Msal_Bot2012_COI	MB49	1	BB80211_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80211	Sample	BB80211	targeted	Absent	Sample	Msal_Bot2012_COI	MB49	COI	45	BB80211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB49	GYTAGTTCCRATGATGTTAGCTG	144.90817
BB80213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86038	WGS84	BB80213	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.135	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80213_Aamu_Bax2006_COI	MB46	1	BB80213_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80213	Sample	BB80213	targeted	Present	Sample	Aamu_Bax2006_COI	MB46	COI	45	BB80213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB46	GCACAACCGGGATCTTTACTTC	144.91026
BB80213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86038	WGS84	BB80213	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80213_Pcan_Bot2011_IGS	MB46	1	BB80213_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80213	Sample	BB80213	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB46	IGS	45	BB80213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB46	CGTAATCCTCAGTACTGGCTA	144.91026
BB80213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86038	WGS84	BB80213	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.81069565	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80213_Sspa_Oph2007_COI	MB46	1	BB80213_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80213	Sample	BB80213	targeted	Present	Sample	Sspa_Oph2007_COI	MB46	COI	45	BB80213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB46	GAAGGCCTTCCGATGATC	144.91026
BB80213	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86038	WGS84	BB80213	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.173	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80213_Upin_Bot2011_COI	MB46	1	BB80213_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80213	Sample	BB80213	targeted	Present	Sample	Upin_Bot2011_COI	MB46	COI	45	BB80213	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB46	CTTTAATTACAGCGTTTTTATTGTTGT	144.91026
BB80214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86081	WGS84	BB80214	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80214_Mgig_Bot2012_COI	MB43	1	BB80214_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80214	Sample	BB80214	targeted	Absent	Sample	Mgig_Bot2012_COI	MB43	COI	45	BB80214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB43	TCTTATTCGTTGGAGACTTTATAACCCT	144.91408
BB80215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86302	WGS84	BB80215	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80215_Cmae_Bot2011_COI	MB39	1	BB80215_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80215	Sample	BB80215	targeted	Absent	Sample	Cmae_Bot2011_COI	MB39	COI	45	BB80215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB39	ATGAACAGTCTATCCTCCTTTAG	144.9144
BB80215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86302	WGS84	BB80215	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80215_Msal_Bot2012_COI	MB39	1	BB80215_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80215	Sample	BB80215	targeted	Absent	Sample	Msal_Bot2012_COI	MB39	COI	45	BB80215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB39	GYTAGTTCCRATGATGTTAGCTG	144.9144
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.095	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80217_Aamu_Bax2006_COI	MB33	1	BB80217_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Present	Sample	Aamu_Bax2006_COI	MB33	COI	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	GCACAACCGGGATCTTTACTTC	144.91683
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80217_Pcan_Bot2011_IGS	MB33	1	BB80217_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB33	IGS	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	CGTAATCCTCAGTACTGGCTA	144.91683
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.27227402	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80217_Sspa_Oph2007_COI	MB33	1	BB80217_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Present	Sample	Sspa_Oph2007_COI	MB33	COI	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	GAAGGCCTTCCGATGATC	144.91683
BB80217	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86439	WGS84	BB80217	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.28	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80217_Upin_Bot2011_COI	MB33	1	BB80217_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80217	Sample	BB80217	targeted	Present	Sample	Upin_Bot2011_COI	MB33	COI	45	BB80217	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB33	CTTTAATTACAGCGTTTTTATTGTTGT	144.91683
BB80218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.86485	WGS84	BB80218	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80218_Mgig_Bot2012_COI	MB32	1	BB80218_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80218	Sample	BB80218	targeted	Absent	Sample	Mgig_Bot2012_COI	MB32	COI	45	BB80218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB32	TCTTATTCGTTGGAGACTTTATAACCCT	144.91483
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80219_Cmae_Bot2011_COI	MB2	1	BB80219_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Absent	Sample	Cmae_Bot2011_COI	MB2	COI	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	ATGAACAGTCTATCCTCCTTTAG	144.90381
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80219_Hsan_Wil2021_COI	MB2	1	BB80219_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Absent	Sample	Hsan_Wil2021_COI	MB2	COI	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	CCTGGGCCGGTATAGTAGGT	144.90381
BB80219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.83986	WGS84	BB80219	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80219_Msal_Bot2012_COI	MB2	1	BB80219_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80219	Sample	BB80219	targeted	Absent	Sample	Msal_Bot2012_COI	MB2	COI	45	BB80219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB2	GYTAGTTCCRATGATGTTAGCTG	144.90381
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.687	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80220_Aamu_Bax2006_COI	MB9	1	BB80220_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Present	Sample	Aamu_Bax2006_COI	MB9	COI	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	GCACAACCGGGATCTTTACTTC	144.90576
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80220_Pcan_Bot2011_IGS	MB9	1	BB80220_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB9	IGS	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	CGTAATCCTCAGTACTGGCTA	144.90576
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.12375641	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80220_Sspa_Oph2007_COI	MB9	1	BB80220_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Present	Sample	Sspa_Oph2007_COI	MB9	COI	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	GAAGGCCTTCCGATGATC	144.90576
BB80220	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84399	WGS84	BB80220	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.005	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80220_Upin_Bot2011_COI	MB9	1	BB80220_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80220	Sample	BB80220	targeted	Present	Sample	Upin_Bot2011_COI	MB9	COI	45	BB80220	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB9	CTTTAATTACAGCGTTTTTATTGTTGT	144.90576
BB80221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84295	WGS84	BB80221	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80221_Mgig_Bot2012_COI	MB7	1	BB80221_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80221	Sample	BB80221	targeted	Absent	Sample	Mgig_Bot2012_COI	MB7	COI	45	BB80221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB7	TCTTATTCGTTGGAGACTTTATAACCCT	144.90603
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80222_Cmae_Bot2011_COI	MB16	1	BB80222_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Absent	Sample	Cmae_Bot2011_COI	MB16	COI	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	ATGAACAGTCTATCCTCCTTTAG	144.91447
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	33.714	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80222_Hsan_Wil2021_COI	MB16	1	BB80222_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Present	Sample	Hsan_Wil2021_COI	MB16	COI	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	CCTGGGCCGGTATAGTAGGT	144.91447
BB80222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84966	WGS84	BB80222	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80222_Msal_Bot2012_COI	MB16	1	BB80222_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80222	Sample	BB80222	targeted	Absent	Sample	Msal_Bot2012_COI	MB16	COI	45	BB80222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB16	GYTAGTTCCRATGATGTTAGCTG	144.91447
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.655	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80224_Aamu_Bax2006_COI	MB12	1	BB80224_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Present	Sample	Aamu_Bax2006_COI	MB12	COI	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	GCACAACCGGGATCTTTACTTC	144.90642
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80224_Pcan_Bot2011_IGS	MB12	1	BB80224_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB12	IGS	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	CGTAATCCTCAGTACTGGCTA	144.90642
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.98141098	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80224_Sspa_Oph2007_COI	MB12	1	BB80224_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Present	Sample	Sspa_Oph2007_COI	MB12	COI	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	GAAGGCCTTCCGATGATC	144.90642
BB80224	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84764	WGS84	BB80224	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.613	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80224_Upin_Bot2011_COI	MB12	1	BB80224_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80224	Sample	BB80224	targeted	Present	Sample	Upin_Bot2011_COI	MB12	COI	45	BB80224	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB12	CTTTAATTACAGCGTTTTTATTGTTGT	144.90642
BB80225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84554	WGS84	BB80225	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80225_Mgig_Bot2012_COI	MB11	1	BB80225_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80225	Sample	BB80225	targeted	Absent	Sample	Mgig_Bot2012_COI	MB11	COI	45	BB80225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB11	TCTTATTCGTTGGAGACTTTATAACCCT	144.90635
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80226_Cmae_Bot2011_COI	MB28	1	BB80226_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Absent	Sample	Cmae_Bot2011_COI	MB28	COI	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	ATGAACAGTCTATCCTCCTTTAG	144.9324
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB80226_Hsan_Wil2021_COI	MB28	1	BB80226_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Absent	Sample	Hsan_Wil2021_COI	MB28	COI	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	CCTGGGCCGGTATAGTAGGT	144.9324
BB80226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84498	WGS84	BB80226	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80226_Msal_Bot2012_COI	MB28	1	BB80226_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80226	Sample	BB80226	targeted	Absent	Sample	Msal_Bot2012_COI	MB28	COI	45	BB80226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB28	GYTAGTTCCRATGATGTTAGCTG	144.9324
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.411	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80228_Aamu_Bax2006_COI	MB23	1	BB80228_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Present	Sample	Aamu_Bax2006_COI	MB23	COI	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	GCACAACCGGGATCTTTACTTC	144.92963
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80228_Pcan_Bot2011_IGS	MB23	1	BB80228_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Absent	Sample	Pcan_Bot2011_IGS	MB23	IGS	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	CGTAATCCTCAGTACTGGCTA	144.92963
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.22533417	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80228_Sspa_Oph2007_COI	MB23	1	BB80228_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Present	Sample	Sspa_Oph2007_COI	MB23	COI	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	GAAGGCCTTCCGATGATC	144.92963
BB80228	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84283	WGS84	BB80228	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.65	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80228_Upin_Bot2011_COI	MB23	1	BB80228_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80228	Sample	BB80228	targeted	Present	Sample	Upin_Bot2011_COI	MB23	COI	45	BB80228	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB23	CTTTAATTACAGCGTTTTTATTGTTGT	144.92963
BB80229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10150	-37.84138	WGS84	BB80229	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80229_Mgig_Bot2012_COI	MB22	1	BB80229_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80229	Sample	BB80229	targeted	Absent	Sample	Mgig_Bot2012_COI	MB22	COI	45	BB80229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MB22	TCTTATTCGTTGGAGACTTTATAACCCT	144.92823
BB80231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84499	WGS84	BB80231	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.652	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80231_Aamu_Bax2006_COI	HB20	1	BB80231_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80231	Sample	BB80231	targeted	Present	Sample	Aamu_Bax2006_COI	HB20	COI	45	BB80231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB20	GCACAACCGGGATCTTTACTTC	147.32948
BB80231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84499	WGS84	BB80231	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80231_Pcan_Bot2011_IGS	HB20	1	BB80231_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80231	Sample	BB80231	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB20	IGS	45	BB80231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB20	CGTAATCCTCAGTACTGGCTA	147.32948
BB80231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84499	WGS84	BB80231	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80231_Sspa_Oph2007_COI	HB20	1	BB80231_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80231	Sample	BB80231	targeted	Absent	Sample	Sspa_Oph2007_COI	HB20	COI	45	BB80231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB20	GAAGGCCTTCCGATGATC	147.32948
BB80231	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84499	WGS84	BB80231	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80231_Upin_Bot2011_COI	HB20	1	BB80231_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80231	Sample	BB80231	targeted	Absent	Sample	Upin_Bot2011_COI	HB20	COI	45	BB80231	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB20	CTTTAATTACAGCGTTTTTATTGTTGT	147.32948
BB80232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88741	WGS84	BB80232	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.869	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80232_Mgig_Bot2012_COI	HB10	1	BB80232_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80232	Sample	BB80232	targeted	Present	Sample	Mgig_Bot2012_COI	HB10	COI	45	BB80232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB10	TCTTATTCGTTGGAGACTTTATAACCCT	147.34018
BB80233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88499	WGS84	BB80233	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80233_Cmae_Bot2011_COI	HB5	1	BB80233_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80233	Sample	BB80233	targeted	Absent	Sample	Cmae_Bot2011_COI	HB5	COI	45	BB80233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB5	ATGAACAGTCTATCCTCCTTTAG	147.33665
BB80233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88499	WGS84	BB80233	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80233_Msal_Bot2012_COI	HB5	1	BB80233_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80233	Sample	BB80233	targeted	Absent	Sample	Msal_Bot2012_COI	HB5	COI	45	BB80233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB5	GYTAGTTCCRATGATGTTAGCTG	147.33665
BB80239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88538	WGS84	BB80239	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.555	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80239_Aamu_Bax2006_COI	HB9	1	BB80239_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80239	Sample	BB80239	targeted	Present	Sample	Aamu_Bax2006_COI	HB9	COI	45	BB80239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB9	GCACAACCGGGATCTTTACTTC	147.33553
BB80239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88538	WGS84	BB80239	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80239_Pcan_Bot2011_IGS	HB9	1	BB80239_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80239	Sample	BB80239	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB9	IGS	45	BB80239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB9	CGTAATCCTCAGTACTGGCTA	147.33553
BB80239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88538	WGS84	BB80239	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80239_Sspa_Oph2007_COI	HB9	1	BB80239_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80239	Sample	BB80239	targeted	Absent	Sample	Sspa_Oph2007_COI	HB9	COI	45	BB80239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB9	GAAGGCCTTCCGATGATC	147.33553
BB80239	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88538	WGS84	BB80239	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80239_Upin_Bot2011_COI	HB9	1	BB80239_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80239	Sample	BB80239	targeted	Absent	Sample	Upin_Bot2011_COI	HB9	COI	45	BB80239	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB9	CTTTAATTACAGCGTTTTTATTGTTGT	147.33553
BB80240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.885	WGS84	BB80240	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.588	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80240_Cmae_Bot2011_COI	HB8	1	BB80240_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80240	Sample	BB80240	targeted	Present	Sample	Cmae_Bot2011_COI	HB8	COI	45	BB80240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB8	ATGAACAGTCTATCCTCCTTTAG	147.33265
BB80240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.885	WGS84	BB80240	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80240_Msal_Bot2012_COI	HB8	1	BB80240_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80240	Sample	BB80240	targeted	Absent	Sample	Msal_Bot2012_COI	HB8	COI	45	BB80240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB8	GYTAGTTCCRATGATGTTAGCTG	147.33265
BB80242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88453	WGS84	BB80242	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.015	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80242_Aamu_Bax2006_COI	HB6	1	BB80242_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80242	Sample	BB80242	targeted	Present	Sample	Aamu_Bax2006_COI	HB6	COI	45	BB80242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB6	GCACAACCGGGATCTTTACTTC	147.33358
BB80242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88453	WGS84	BB80242	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80242_Pcan_Bot2011_IGS	HB6	1	BB80242_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80242	Sample	BB80242	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB6	IGS	45	BB80242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB6	CGTAATCCTCAGTACTGGCTA	147.33358
BB80242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88453	WGS84	BB80242	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80242_Sspa_Oph2007_COI	HB6	1	BB80242_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80242	Sample	BB80242	targeted	Absent	Sample	Sspa_Oph2007_COI	HB6	COI	45	BB80242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB6	GAAGGCCTTCCGATGATC	147.33358
BB80242	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88453	WGS84	BB80242	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80242_Upin_Bot2011_COI	HB6	1	BB80242_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80242	Sample	BB80242	targeted	Absent	Sample	Upin_Bot2011_COI	HB6	COI	45	BB80242	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB6	CTTTAATTACAGCGTTTTTATTGTTGT	147.33358
BB80243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88098	WGS84	BB80243	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80243_Mgig_Bot2012_COI	HB13	1	BB80243_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80243	Sample	BB80243	targeted	Absent	Sample	Mgig_Bot2012_COI	HB13	COI	45	BB80243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB13	TCTTATTCGTTGGAGACTTTATAACCCT	147.34197
BB80244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88586	WGS84	BB80244	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80244_Cmae_Bot2011_COI	HB1	1	BB80244_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80244	Sample	BB80244	targeted	Absent	Sample	Cmae_Bot2011_COI	HB1	COI	45	BB80244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB1	ATGAACAGTCTATCCTCCTTTAG	147.33887
BB80244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.88586	WGS84	BB80244	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80244_Msal_Bot2012_COI	HB1	1	BB80244_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80244	Sample	BB80244	targeted	Absent	Sample	Msal_Bot2012_COI	HB1	COI	45	BB80244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB1	GYTAGTTCCRATGATGTTAGCTG	147.33887
BB80246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84502	WGS84	BB80246	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.171	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80246_Aamu_Bax2006_COI	HB18	1	BB80246_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80246	Sample	BB80246	targeted	Present	Sample	Aamu_Bax2006_COI	HB18	COI	45	BB80246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB18	GCACAACCGGGATCTTTACTTC	147.33073
BB80246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84502	WGS84	BB80246	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80246_Pcan_Bot2011_IGS	HB18	1	BB80246_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80246	Sample	BB80246	targeted	Absent	Sample	Pcan_Bot2011_IGS	HB18	IGS	45	BB80246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB18	CGTAATCCTCAGTACTGGCTA	147.33073
BB80246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84502	WGS84	BB80246	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80246_Sspa_Oph2007_COI	HB18	1	BB80246_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80246	Sample	BB80246	targeted	Absent	Sample	Sspa_Oph2007_COI	HB18	COI	45	BB80246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB18	GAAGGCCTTCCGATGATC	147.33073
BB80246	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84502	WGS84	BB80246	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80246_Upin_Bot2011_COI	HB18	1	BB80246_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80246	Sample	BB80246	targeted	Absent	Sample	Upin_Bot2011_COI	HB18	COI	45	BB80246	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB18	CTTTAATTACAGCGTTTTTATTGTTGT	147.33073
BB80247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84651	WGS84	BB80247	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.035	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80247_Mgig_Bot2012_COI	HB16	1	BB80247_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80247	Sample	BB80247	targeted	Present	Sample	Mgig_Bot2012_COI	HB16	COI	45	BB80247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB16	TCTTATTCGTTGGAGACTTTATAACCCT	147.32893
BB80248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84752	WGS84	BB80248	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.295	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80248_Cmae_Bot2011_COI	HB14	1	BB80248_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80248	Sample	BB80248	targeted	Present	Sample	Cmae_Bot2011_COI	HB14	COI	45	BB80248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB14	ATGAACAGTCTATCCTCCTTTAG	147.32763
BB80248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.84752	WGS84	BB80248	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80248_Msal_Bot2012_COI	HB14	1	BB80248_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80248	Sample	BB80248	targeted	Absent	Sample	Msal_Bot2012_COI	HB14	COI	45	BB80248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB14	GYTAGTTCCRATGATGTTAGCTG	147.32763
BB80250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10149	-42.82857	WGS84	BB80250	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.109	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80250_Mgig_Bot2012_COI	HB22	1	BB80250_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80250	Sample	BB80250	targeted	Present	Sample	Mgig_Bot2012_COI	HB22	COI	45	BB80250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HB22	TCTTATTCGTTGGAGACTTTATAACCCT	147.31482
BB80251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81897	WGS84	BB80251	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80251_Cmae_Bot2011_COI	GB110	1	BB80251_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80251	Sample	BB80251	targeted	Absent	Sample	Cmae_Bot2011_COI	GB110	COI	45	BB80251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB110	ATGAACAGTCTATCCTCCTTTAG	151.23877
BB80251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81897	WGS84	BB80251	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80251_Msal_Bot2012_COI	GB110	1	BB80251_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80251	Sample	BB80251	targeted	Absent	Sample	Msal_Bot2012_COI	GB110	COI	45	BB80251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB110	GYTAGTTCCRATGATGTTAGCTG	151.23877
BB80253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79251	WGS84	BB80253	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80253_Aamu_Bax2006_COI	GB60	1	BB80253_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80253	Sample	BB80253	targeted	Absent	Sample	Aamu_Bax2006_COI	GB60	COI	45	BB80253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB60	GCACAACCGGGATCTTTACTTC	151.18214
BB80253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79251	WGS84	BB80253	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80253_Pcan_Bot2011_IGS	GB60	1	BB80253_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80253	Sample	BB80253	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB60	IGS	45	BB80253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB60	CGTAATCCTCAGTACTGGCTA	151.18214
BB80253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79251	WGS84	BB80253	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80253_Sspa_Oph2007_COI	GB60	1	BB80253_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80253	Sample	BB80253	targeted	Absent	Sample	Sspa_Oph2007_COI	GB60	COI	45	BB80253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB60	GAAGGCCTTCCGATGATC	151.18214
BB80253	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79251	WGS84	BB80253	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80253_Upin_Bot2011_COI	GB60	1	BB80253_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80253	Sample	BB80253	targeted	Absent	Sample	Upin_Bot2011_COI	GB60	COI	45	BB80253	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB60	CTTTAATTACAGCGTTTTTATTGTTGT	151.18214
BB80254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78354	WGS84	BB80254	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80254_Mgig_Bot2012_COI	GB90	1	BB80254_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80254	Sample	BB80254	targeted	Absent	Sample	Mgig_Bot2012_COI	GB90	COI	45	BB80254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB90	TCTTATTCGTTGGAGACTTTATAACCCT	151.20098
BB80255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77796	WGS84	BB80255	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80255_Cmae_Bot2011_COI	GB85	1	BB80255_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80255	Sample	BB80255	targeted	Absent	Sample	Cmae_Bot2011_COI	GB85	COI	45	BB80255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB85	ATGAACAGTCTATCCTCCTTTAG	151.19555
BB80255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.77796	WGS84	BB80255	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80255_Msal_Bot2012_COI	GB85	1	BB80255_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80255	Sample	BB80255	targeted	Absent	Sample	Msal_Bot2012_COI	GB85	COI	45	BB80255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB85	GYTAGTTCCRATGATGTTAGCTG	151.19555
BB80257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82328	WGS84	BB80257	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80257_Aamu_Bax2006_COI	GB70	1	BB80257_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80257	Sample	BB80257	targeted	Absent	Sample	Aamu_Bax2006_COI	GB70	COI	45	BB80257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB70	GCACAACCGGGATCTTTACTTC	151.24379
BB80257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82328	WGS84	BB80257	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80257_Pcan_Bot2011_IGS	GB70	1	BB80257_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80257	Sample	BB80257	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB70	IGS	45	BB80257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB70	CGTAATCCTCAGTACTGGCTA	151.24379
BB80257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82328	WGS84	BB80257	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80257_Sspa_Oph2007_COI	GB70	1	BB80257_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80257	Sample	BB80257	targeted	Absent	Sample	Sspa_Oph2007_COI	GB70	COI	45	BB80257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB70	GAAGGCCTTCCGATGATC	151.24379
BB80257	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82328	WGS84	BB80257	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80257_Upin_Bot2011_COI	GB70	1	BB80257_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80257	Sample	BB80257	targeted	Absent	Sample	Upin_Bot2011_COI	GB70	COI	45	BB80257	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB70	CTTTAATTACAGCGTTTTTATTGTTGT	151.24379
BB80258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78741	WGS84	BB80258	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80258_Mgig_Bot2012_COI	GB50	1	BB80258_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80258	Sample	BB80258	targeted	Absent	Sample	Mgig_Bot2012_COI	GB50	COI	45	BB80258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB50	TCTTATTCGTTGGAGACTTTATAACCCT	151.1737
BB80259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.7849	WGS84	BB80259	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80259_Cmae_Bot2011_COI	GB45	1	BB80259_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80259	Sample	BB80259	targeted	Absent	Sample	Cmae_Bot2011_COI	GB45	COI	45	BB80259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB45	ATGAACAGTCTATCCTCCTTTAG	151.17149
BB80259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.7849	WGS84	BB80259	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80259_Msal_Bot2012_COI	GB45	1	BB80259_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80259	Sample	BB80259	targeted	Absent	Sample	Msal_Bot2012_COI	GB45	COI	45	BB80259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB45	GYTAGTTCCRATGATGTTAGCTG	151.17149
BB80260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80815	WGS84	BB80260	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80260_Aamu_Bax2006_COI	GB40	1	BB80260_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80260	Sample	BB80260	targeted	Absent	Sample	Aamu_Bax2006_COI	GB40	COI	45	BB80260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB40	GCACAACCGGGATCTTTACTTC	151.21644
BB80260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80815	WGS84	BB80260	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80260_Pcan_Bot2011_IGS	GB40	1	BB80260_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80260	Sample	BB80260	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB40	IGS	45	BB80260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB40	CGTAATCCTCAGTACTGGCTA	151.21644
BB80260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80815	WGS84	BB80260	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80260_Sspa_Oph2007_COI	GB40	1	BB80260_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80260	Sample	BB80260	targeted	Absent	Sample	Sspa_Oph2007_COI	GB40	COI	45	BB80260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB40	GAAGGCCTTCCGATGATC	151.21644
BB80260	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80815	WGS84	BB80260	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80260_Upin_Bot2011_COI	GB40	1	BB80260_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80260	Sample	BB80260	targeted	Absent	Sample	Upin_Bot2011_COI	GB40	COI	45	BB80260	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB40	CTTTAATTACAGCGTTTTTATTGTTGT	151.21644
BB80261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8056	WGS84	BB80261	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80261_Mgig_Bot2012_COI	GB35	1	BB80261_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80261	Sample	BB80261	targeted	Absent	Sample	Mgig_Bot2012_COI	GB35	COI	45	BB80261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB35	TCTTATTCGTTGGAGACTTTATAACCCT	151.21581
BB80262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83508	WGS84	BB80262	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80262_Cmae_Bot2011_COI	GB20	1	BB80262_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80262	Sample	BB80262	targeted	Absent	Sample	Cmae_Bot2011_COI	GB20	COI	45	BB80262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB20	ATGAACAGTCTATCCTCCTTTAG	151.26929
BB80262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83508	WGS84	BB80262	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80262_Msal_Bot2012_COI	GB20	1	BB80262_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80262	Sample	BB80262	targeted	Absent	Sample	Msal_Bot2012_COI	GB20	COI	45	BB80262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB20	GYTAGTTCCRATGATGTTAGCTG	151.26929
BB80264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85116	WGS84	BB80264	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80264_Aamu_Bax2006_COI	GB10	1	BB80264_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80264	Sample	BB80264	targeted	Absent	Sample	Aamu_Bax2006_COI	GB10	COI	45	BB80264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB10	GCACAACCGGGATCTTTACTTC	151.31425
BB80264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85116	WGS84	BB80264	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80264_Pcan_Bot2011_IGS	GB10	1	BB80264_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80264	Sample	BB80264	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB10	IGS	45	BB80264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB10	CGTAATCCTCAGTACTGGCTA	151.31425
BB80264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85116	WGS84	BB80264	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80264_Sspa_Oph2007_COI	GB10	1	BB80264_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80264	Sample	BB80264	targeted	Absent	Sample	Sspa_Oph2007_COI	GB10	COI	45	BB80264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB10	GAAGGCCTTCCGATGATC	151.31425
BB80264	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85116	WGS84	BB80264	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80264_Upin_Bot2011_COI	GB10	1	BB80264_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80264	Sample	BB80264	targeted	Absent	Sample	Upin_Bot2011_COI	GB10	COI	45	BB80264	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB10	CTTTAATTACAGCGTTTTTATTGTTGT	151.31425
BB80265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.853	WGS84	BB80265	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80265_Mgig_Bot2012_COI	GB5	1	BB80265_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80265	Sample	BB80265	targeted	Absent	Sample	Mgig_Bot2012_COI	GB5	COI	45	BB80265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB5	TCTTATTCGTTGGAGACTTTATAACCCT	151.32026
BB80271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80759	WGS84	BB80271	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80271_Aamu_Bax2006_COI	GB43	1	BB80271_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80271	Sample	BB80271	targeted	Absent	Sample	Aamu_Bax2006_COI	GB43	COI	45	BB80271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB43	GCACAACCGGGATCTTTACTTC	151.22026
BB80271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80759	WGS84	BB80271	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80271_Pcan_Bot2011_IGS	GB43	1	BB80271_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80271	Sample	BB80271	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB43	IGS	45	BB80271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB43	CGTAATCCTCAGTACTGGCTA	151.22026
BB80271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80759	WGS84	BB80271	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80271_Sspa_Oph2007_COI	GB43	1	BB80271_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80271	Sample	BB80271	targeted	Absent	Sample	Sspa_Oph2007_COI	GB43	COI	45	BB80271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB43	GAAGGCCTTCCGATGATC	151.22026
BB80271	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80759	WGS84	BB80271	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80271_Upin_Bot2011_COI	GB43	1	BB80271_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80271	Sample	BB80271	targeted	Absent	Sample	Upin_Bot2011_COI	GB43	COI	45	BB80271	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB43	CTTTAATTACAGCGTTTTTATTGTTGT	151.22026
BB80272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80744	WGS84	BB80272	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80272_Mgig_Bot2012_COI	GB42	1	BB80272_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80272	Sample	BB80272	targeted	Absent	Sample	Mgig_Bot2012_COI	GB42	COI	45	BB80272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB42	TCTTATTCGTTGGAGACTTTATAACCCT	151.21814
BB80273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80713	WGS84	BB80273	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80273_Cmae_Bot2011_COI	GB38	1	BB80273_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80273	Sample	BB80273	targeted	Absent	Sample	Cmae_Bot2011_COI	GB38	COI	45	BB80273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB38	ATGAACAGTCTATCCTCCTTTAG	151.21483
BB80273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.80713	WGS84	BB80273	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80273_Msal_Bot2012_COI	GB38	1	BB80273_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80273	Sample	BB80273	targeted	Absent	Sample	Msal_Bot2012_COI	GB38	COI	45	BB80273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB38	GYTAGTTCCRATGATGTTAGCTG	151.21483
BB80275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79118	WGS84	BB80275	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80275_Aamu_Bax2006_COI	GB63	1	BB80275_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80275	Sample	BB80275	targeted	Absent	Sample	Aamu_Bax2006_COI	GB63	COI	45	BB80275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB63	GCACAACCGGGATCTTTACTTC	151.17737
BB80275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79118	WGS84	BB80275	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80275_Pcan_Bot2011_IGS	GB63	1	BB80275_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80275	Sample	BB80275	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB63	IGS	45	BB80275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB63	CGTAATCCTCAGTACTGGCTA	151.17737
BB80275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79118	WGS84	BB80275	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80275_Sspa_Oph2007_COI	GB63	1	BB80275_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80275	Sample	BB80275	targeted	Absent	Sample	Sspa_Oph2007_COI	GB63	COI	45	BB80275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB63	GAAGGCCTTCCGATGATC	151.17737
BB80275	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79118	WGS84	BB80275	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80275_Upin_Bot2011_COI	GB63	1	BB80275_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80275	Sample	BB80275	targeted	Absent	Sample	Upin_Bot2011_COI	GB63	COI	45	BB80275	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB63	CTTTAATTACAGCGTTTTTATTGTTGT	151.17737
BB80276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79094	WGS84	BB80276	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80276_Mgig_Bot2012_COI	GB58	1	BB80276_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80276	Sample	BB80276	targeted	Absent	Sample	Mgig_Bot2012_COI	GB58	COI	45	BB80276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB58	TCTTATTCGTTGGAGACTTTATAACCCT	151.1826
BB80277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78985	WGS84	BB80277	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80277_Cmae_Bot2011_COI	GB57	1	BB80277_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80277	Sample	BB80277	targeted	Absent	Sample	Cmae_Bot2011_COI	GB57	COI	45	BB80277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB57	ATGAACAGTCTATCCTCCTTTAG	151.18016
BB80277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78985	WGS84	BB80277	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80277_Msal_Bot2012_COI	GB57	1	BB80277_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80277	Sample	BB80277	targeted	Absent	Sample	Msal_Bot2012_COI	GB57	COI	45	BB80277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB57	GYTAGTTCCRATGATGTTAGCTG	151.18016
BB80279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78885	WGS84	BB80279	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80279_Aamu_Bax2006_COI	GB54	1	BB80279_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80279	Sample	BB80279	targeted	Absent	Sample	Aamu_Bax2006_COI	GB54	COI	45	BB80279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB54	GCACAACCGGGATCTTTACTTC	151.17702
BB80279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78885	WGS84	BB80279	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80279_Pcan_Bot2011_IGS	GB54	1	BB80279_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80279	Sample	BB80279	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB54	IGS	45	BB80279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB54	CGTAATCCTCAGTACTGGCTA	151.17702
BB80279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78885	WGS84	BB80279	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80279_Sspa_Oph2007_COI	GB54	1	BB80279_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80279	Sample	BB80279	targeted	Absent	Sample	Sspa_Oph2007_COI	GB54	COI	45	BB80279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB54	GAAGGCCTTCCGATGATC	151.17702
BB80279	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78885	WGS84	BB80279	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80279_Upin_Bot2011_COI	GB54	1	BB80279_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80279	Sample	BB80279	targeted	Absent	Sample	Upin_Bot2011_COI	GB54	COI	45	BB80279	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB54	CTTTAATTACAGCGTTTTTATTGTTGT	151.17702
BB80280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78708	WGS84	BB80280	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80280_Cmae_Bot2011_COI	GB52	1	BB80280_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80280	Sample	BB80280	targeted	Absent	Sample	Cmae_Bot2011_COI	GB52	COI	45	BB80280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB52	ATGAACAGTCTATCCTCCTTTAG	151.17666
BB80280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78708	WGS84	BB80280	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80280_Msal_Bot2012_COI	GB52	1	BB80280_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80280	Sample	BB80280	targeted	Absent	Sample	Msal_Bot2012_COI	GB52	COI	45	BB80280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB52	GYTAGTTCCRATGATGTTAGCTG	151.17666
BB80282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80282	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80282_Aamu_Bax2006_COI	GB46	1	BB80282_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80282	Sample	BB80282	targeted	Absent	Sample	Aamu_Bax2006_COI	GB46	COI	45	BB80282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB46	GCACAACCGGGATCTTTACTTC	151.17273
BB80282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80282	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80282_Pcan_Bot2011_IGS	GB46	1	BB80282_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80282	Sample	BB80282	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB46	IGS	45	BB80282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB46	CGTAATCCTCAGTACTGGCTA	151.17273
BB80282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80282	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80282_Sspa_Oph2007_COI	GB46	1	BB80282_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80282	Sample	BB80282	targeted	Absent	Sample	Sspa_Oph2007_COI	GB46	COI	45	BB80282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB46	GAAGGCCTTCCGATGATC	151.17273
BB80282	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80282	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80282_Upin_Bot2011_COI	GB46	1	BB80282_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80282	Sample	BB80282	targeted	Absent	Sample	Upin_Bot2011_COI	GB46	COI	45	BB80282	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB46	CTTTAATTACAGCGTTTTTATTGTTGT	151.17273
BB80283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78523	WGS84	BB80283	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80283_Mgig_Bot2012_COI	GB92	1	BB80283_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80283	Sample	BB80283	targeted	Absent	Sample	Mgig_Bot2012_COI	GB92	COI	45	BB80283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB92	TCTTATTCGTTGGAGACTTTATAACCCT	151.20462
BB80284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78078	WGS84	BB80284	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80284_Cmae_Bot2011_COI	GB89	1	BB80284_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80284	Sample	BB80284	targeted	Absent	Sample	Cmae_Bot2011_COI	GB89	COI	45	BB80284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB89	ATGAACAGTCTATCCTCCTTTAG	151.19976
BB80284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.78078	WGS84	BB80284	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80284_Msal_Bot2012_COI	GB89	1	BB80284_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80284	Sample	BB80284	targeted	Absent	Sample	Msal_Bot2012_COI	GB89	COI	45	BB80284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB89	GYTAGTTCCRATGATGTTAGCTG	151.19976
BB80286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81603	WGS84	BB80286	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80286_Aamu_Bax2006_COI	GB82	1	BB80286_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80286	Sample	BB80286	targeted	Absent	Sample	Aamu_Bax2006_COI	GB82	COI	45	BB80286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB82	GCACAACCGGGATCTTTACTTC	151.2366
BB80286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81603	WGS84	BB80286	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80286_Pcan_Bot2011_IGS	GB82	1	BB80286_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80286	Sample	BB80286	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB82	IGS	45	BB80286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB82	CGTAATCCTCAGTACTGGCTA	151.2366
BB80286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81603	WGS84	BB80286	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80286_Sspa_Oph2007_COI	GB82	1	BB80286_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80286	Sample	BB80286	targeted	Absent	Sample	Sspa_Oph2007_COI	GB82	COI	45	BB80286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB82	GAAGGCCTTCCGATGATC	151.2366
BB80286	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81603	WGS84	BB80286	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80286_Upin_Bot2011_COI	GB82	1	BB80286_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80286	Sample	BB80286	targeted	Absent	Sample	Upin_Bot2011_COI	GB82	COI	45	BB80286	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB82	CTTTAATTACAGCGTTTTTATTGTTGT	151.2366
BB80287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81718	WGS84	BB80287	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80287_Mgig_Bot2012_COI	GB84	1	BB80287_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80287	Sample	BB80287	targeted	Absent	Sample	Mgig_Bot2012_COI	GB84	COI	45	BB80287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB84	TCTTATTCGTTGGAGACTTTATAACCCT	151.23821
BB80288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82325	WGS84	BB80288	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80288_Cmae_Bot2011_COI	GB98	1	BB80288_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80288	Sample	BB80288	targeted	Absent	Sample	Cmae_Bot2011_COI	GB98	COI	45	BB80288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB98	ATGAACAGTCTATCCTCCTTTAG	151.24116
BB80288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82325	WGS84	BB80288	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80288_Msal_Bot2012_COI	GB98	1	BB80288_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80288	Sample	BB80288	targeted	Absent	Sample	Msal_Bot2012_COI	GB98	COI	45	BB80288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB98	GYTAGTTCCRATGATGTTAGCTG	151.24116
BB80290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81831	WGS84	BB80290	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80290_Mgig_Bot2012_COI	GB77	1	BB80290_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80290	Sample	BB80290	targeted	Absent	Sample	Mgig_Bot2012_COI	GB77	COI	45	BB80290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB77	TCTTATTCGTTGGAGACTTTATAACCCT	151.24192
BB80291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82048	WGS84	BB80291	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80291_Cmae_Bot2011_COI	GB76	1	BB80291_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80291	Sample	BB80291	targeted	Absent	Sample	Cmae_Bot2011_COI	GB76	COI	45	BB80291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB76	ATGAACAGTCTATCCTCCTTTAG	151.24452
BB80291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82048	WGS84	BB80291	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80291_Msal_Bot2012_COI	GB76	1	BB80291_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80291	Sample	BB80291	targeted	Absent	Sample	Msal_Bot2012_COI	GB76	COI	45	BB80291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB76	GYTAGTTCCRATGATGTTAGCTG	151.24452
BB80293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82236	WGS84	BB80293	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80293_Aamu_Bax2006_COI	GB73	1	BB80293_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80293	Sample	BB80293	targeted	Absent	Sample	Aamu_Bax2006_COI	GB73	COI	45	BB80293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB73	GCACAACCGGGATCTTTACTTC	151.24253
BB80293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82236	WGS84	BB80293	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80293_Pcan_Bot2011_IGS	GB73	1	BB80293_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80293	Sample	BB80293	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB73	IGS	45	BB80293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB73	CGTAATCCTCAGTACTGGCTA	151.24253
BB80293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82236	WGS84	BB80293	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80293_Sspa_Oph2007_COI	GB73	1	BB80293_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80293	Sample	BB80293	targeted	Absent	Sample	Sspa_Oph2007_COI	GB73	COI	45	BB80293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB73	GAAGGCCTTCCGATGATC	151.24253
BB80293	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82236	WGS84	BB80293	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80293_Upin_Bot2011_COI	GB73	1	BB80293_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80293	Sample	BB80293	targeted	Absent	Sample	Upin_Bot2011_COI	GB73	COI	45	BB80293	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB73	CTTTAATTACAGCGTTTTTATTGTTGT	151.24253
BB80294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82273	WGS84	BB80294	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80294_Mgig_Bot2012_COI	GB69	1	BB80294_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80294	Sample	BB80294	targeted	Absent	Sample	Mgig_Bot2012_COI	GB69	COI	45	BB80294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB69	TCTTATTCGTTGGAGACTTTATAACCCT	151.24409
BB80295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8244	WGS84	BB80295	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80295_Cmae_Bot2011_COI	GB68	1	BB80295_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80295	Sample	BB80295	targeted	Absent	Sample	Cmae_Bot2011_COI	GB68	COI	45	BB80295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB68	ATGAACAGTCTATCCTCCTTTAG	151.24366
BB80295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8244	WGS84	BB80295	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80295_Msal_Bot2012_COI	GB68	1	BB80295_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80295	Sample	BB80295	targeted	Absent	Sample	Msal_Bot2012_COI	GB68	COI	45	BB80295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB68	GYTAGTTCCRATGATGTTAGCTG	151.24366
BB80297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82526	WGS84	BB80297	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80297_Aamu_Bax2006_COI	GB64	1	BB80297_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80297	Sample	BB80297	targeted	Absent	Sample	Aamu_Bax2006_COI	GB64	COI	45	BB80297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB64	GCACAACCGGGATCTTTACTTC	151.24567
BB80297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82526	WGS84	BB80297	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80297_Pcan_Bot2011_IGS	GB64	1	BB80297_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80297	Sample	BB80297	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB64	IGS	45	BB80297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB64	CGTAATCCTCAGTACTGGCTA	151.24567
BB80297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82526	WGS84	BB80297	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80297_Sspa_Oph2007_COI	GB64	1	BB80297_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80297	Sample	BB80297	targeted	Absent	Sample	Sspa_Oph2007_COI	GB64	COI	45	BB80297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB64	GAAGGCCTTCCGATGATC	151.24567
BB80297	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82526	WGS84	BB80297	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80297_Upin_Bot2011_COI	GB64	1	BB80297_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80297	Sample	BB80297	targeted	Absent	Sample	Upin_Bot2011_COI	GB64	COI	45	BB80297	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB64	CTTTAATTACAGCGTTTTTATTGTTGT	151.24567
BB80298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8268	WGS84	BB80298	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80298_Mgig_Bot2012_COI	GB34	1	BB80298_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80298	Sample	BB80298	targeted	Absent	Sample	Mgig_Bot2012_COI	GB34	COI	45	BB80298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB34	TCTTATTCGTTGGAGACTTTATAACCCT	151.24598
BB80299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82251	WGS84	BB80299	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80299_Cmae_Bot2011_COI	GB33	1	BB80299_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80299	Sample	BB80299	targeted	Absent	Sample	Cmae_Bot2011_COI	GB33	COI	45	BB80299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB33	ATGAACAGTCTATCCTCCTTTAG	151.24644
BB80299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82251	WGS84	BB80299	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80299_Msal_Bot2012_COI	GB33	1	BB80299_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80299	Sample	BB80299	targeted	Absent	Sample	Msal_Bot2012_COI	GB33	COI	45	BB80299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB33	GYTAGTTCCRATGATGTTAGCTG	151.24644
BB80301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82699	WGS84	BB80301	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80301_Aamu_Bax2006_COI	GB29	1	BB80301_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80301	Sample	BB80301	targeted	Absent	Sample	Aamu_Bax2006_COI	GB29	COI	45	BB80301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB29	GCACAACCGGGATCTTTACTTC	151.24773
BB80301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82699	WGS84	BB80301	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80301_Pcan_Bot2011_IGS	GB29	1	BB80301_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80301	Sample	BB80301	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB29	IGS	45	BB80301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB29	CGTAATCCTCAGTACTGGCTA	151.24773
BB80301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82699	WGS84	BB80301	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80301_Sspa_Oph2007_COI	GB29	1	BB80301_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80301	Sample	BB80301	targeted	Absent	Sample	Sspa_Oph2007_COI	GB29	COI	45	BB80301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB29	GAAGGCCTTCCGATGATC	151.24773
BB80301	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82699	WGS84	BB80301	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80301_Upin_Bot2011_COI	GB29	1	BB80301_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80301	Sample	BB80301	targeted	Absent	Sample	Upin_Bot2011_COI	GB29	COI	45	BB80301	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB29	CTTTAATTACAGCGTTTTTATTGTTGT	151.24773
BB80302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81859	WGS84	BB80302	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80302_Mgig_Bot2012_COI	GB109	1	BB80302_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80302	Sample	BB80302	targeted	Absent	Sample	Mgig_Bot2012_COI	GB109	COI	45	BB80302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB109	TCTTATTCGTTGGAGACTTTATAACCCT	151.23987
BB80303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82008	WGS84	BB80303	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80303_Cmae_Bot2011_COI	GB107	1	BB80303_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80303	Sample	BB80303	targeted	Absent	Sample	Cmae_Bot2011_COI	GB107	COI	45	BB80303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB107	ATGAACAGTCTATCCTCCTTTAG	151.23915
BB80303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82008	WGS84	BB80303	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80303_Msal_Bot2012_COI	GB107	1	BB80303_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80303	Sample	BB80303	targeted	Absent	Sample	Msal_Bot2012_COI	GB107	COI	45	BB80303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB107	GYTAGTTCCRATGATGTTAGCTG	151.23915
BB80305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81982	WGS84	BB80305	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80305_Aamu_Bax2006_COI	GB103	1	BB80305_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80305	Sample	BB80305	targeted	Absent	Sample	Aamu_Bax2006_COI	GB103	COI	45	BB80305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB103	GCACAACCGGGATCTTTACTTC	151.24166
BB80305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81982	WGS84	BB80305	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80305_Pcan_Bot2011_IGS	GB103	1	BB80305_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80305	Sample	BB80305	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB103	IGS	45	BB80305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB103	CGTAATCCTCAGTACTGGCTA	151.24166
BB80305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81982	WGS84	BB80305	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80305_Sspa_Oph2007_COI	GB103	1	BB80305_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80305	Sample	BB80305	targeted	Absent	Sample	Sspa_Oph2007_COI	GB103	COI	45	BB80305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB103	GAAGGCCTTCCGATGATC	151.24166
BB80305	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.81982	WGS84	BB80305	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80305_Upin_Bot2011_COI	GB103	1	BB80305_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80305	Sample	BB80305	targeted	Absent	Sample	Upin_Bot2011_COI	GB103	COI	45	BB80305	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB103	CTTTAATTACAGCGTTTTTATTGTTGT	151.24166
BB80306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.82155	WGS84	BB80306	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80306_Mgig_Bot2012_COI	GB102	1	BB80306_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80306	Sample	BB80306	targeted	Absent	Sample	Mgig_Bot2012_COI	GB102	COI	45	BB80306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB102	TCTTATTCGTTGGAGACTTTATAACCCT	151.24001
BB80307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79354	WGS84	BB80307	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80307_Cmae_Bot2011_COI	GB96	1	BB80307_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80307	Sample	BB80307	targeted	Absent	Sample	Cmae_Bot2011_COI	GB96	COI	45	BB80307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB96	ATGAACAGTCTATCCTCCTTTAG	151.21271
BB80307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.79354	WGS84	BB80307	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80307_Msal_Bot2012_COI	GB96	1	BB80307_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80307	Sample	BB80307	targeted	Absent	Sample	Msal_Bot2012_COI	GB96	COI	45	BB80307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB96	GYTAGTTCCRATGATGTTAGCTG	151.21271
BB80309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83073	WGS84	BB80309	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80309_Aamu_Bax2006_COI	GB28	1	BB80309_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80309	Sample	BB80309	targeted	Absent	Sample	Aamu_Bax2006_COI	GB28	COI	45	BB80309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB28	GCACAACCGGGATCTTTACTTC	151.25527
BB80309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83073	WGS84	BB80309	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80309_Pcan_Bot2011_IGS	GB28	1	BB80309_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80309	Sample	BB80309	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB28	IGS	45	BB80309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB28	CGTAATCCTCAGTACTGGCTA	151.25527
BB80309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83073	WGS84	BB80309	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80309_Sspa_Oph2007_COI	GB28	1	BB80309_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80309	Sample	BB80309	targeted	Absent	Sample	Sspa_Oph2007_COI	GB28	COI	45	BB80309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB28	GAAGGCCTTCCGATGATC	151.25527
BB80309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83073	WGS84	BB80309	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80309_Upin_Bot2011_COI	GB28	1	BB80309_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80309	Sample	BB80309	targeted	Absent	Sample	Upin_Bot2011_COI	GB28	COI	45	BB80309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB28	CTTTAATTACAGCGTTTTTATTGTTGT	151.25527
BB80310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83125	WGS84	BB80310	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80310_Cmae_Bot2011_COI	GB27	1	BB80310_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80310	Sample	BB80310	targeted	Absent	Sample	Cmae_Bot2011_COI	GB27	COI	45	BB80310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB27	ATGAACAGTCTATCCTCCTTTAG	151.25757
BB80310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83125	WGS84	BB80310	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80310_Msal_Bot2012_COI	GB27	1	BB80310_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80310	Sample	BB80310	targeted	Absent	Sample	Msal_Bot2012_COI	GB27	COI	45	BB80310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB27	GYTAGTTCCRATGATGTTAGCTG	151.25757
BB80312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83228	WGS84	BB80312	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80312_Aamu_Bax2006_COI	GB24	1	BB80312_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80312	Sample	BB80312	targeted	Absent	Sample	Aamu_Bax2006_COI	GB24	COI	45	BB80312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB24	GCACAACCGGGATCTTTACTTC	151.26328
BB80312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83228	WGS84	BB80312	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80312_Pcan_Bot2011_IGS	GB24	1	BB80312_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80312	Sample	BB80312	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB24	IGS	45	BB80312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB24	CGTAATCCTCAGTACTGGCTA	151.26328
BB80312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83228	WGS84	BB80312	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80312_Sspa_Oph2007_COI	GB24	1	BB80312_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80312	Sample	BB80312	targeted	Absent	Sample	Sspa_Oph2007_COI	GB24	COI	45	BB80312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB24	GAAGGCCTTCCGATGATC	151.26328
BB80312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83228	WGS84	BB80312	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80312_Upin_Bot2011_COI	GB24	1	BB80312_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80312	Sample	BB80312	targeted	Absent	Sample	Upin_Bot2011_COI	GB24	COI	45	BB80312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB24	CTTTAATTACAGCGTTTTTATTGTTGT	151.26328
BB80313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83295	WGS84	BB80313	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80313_Mgig_Bot2012_COI	GB23	1	BB80313_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80313	Sample	BB80313	targeted	Absent	Sample	Mgig_Bot2012_COI	GB23	COI	45	BB80313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB23	TCTTATTCGTTGGAGACTTTATAACCCT	151.2651
BB80314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83452	WGS84	BB80314	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80314_Cmae_Bot2011_COI	GB22	1	BB80314_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80314	Sample	BB80314	targeted	Absent	Sample	Cmae_Bot2011_COI	GB22	COI	45	BB80314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB22	ATGAACAGTCTATCCTCCTTTAG	151.267
BB80314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.83452	WGS84	BB80314	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80314_Msal_Bot2012_COI	GB22	1	BB80314_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80314	Sample	BB80314	targeted	Absent	Sample	Msal_Bot2012_COI	GB22	COI	45	BB80314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB22	GYTAGTTCCRATGATGTTAGCTG	151.267
BB80316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85172	WGS84	BB80316	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80316_Aamu_Bax2006_COI	GB13	1	BB80316_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80316	Sample	BB80316	targeted	Absent	Sample	Aamu_Bax2006_COI	GB13	COI	45	BB80316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB13	GCACAACCGGGATCTTTACTTC	151.30872
BB80316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85172	WGS84	BB80316	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80316_Pcan_Bot2011_IGS	GB13	1	BB80316_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80316	Sample	BB80316	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB13	IGS	45	BB80316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB13	CGTAATCCTCAGTACTGGCTA	151.30872
BB80316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85172	WGS84	BB80316	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80316_Sspa_Oph2007_COI	GB13	1	BB80316_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80316	Sample	BB80316	targeted	Absent	Sample	Sspa_Oph2007_COI	GB13	COI	45	BB80316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB13	GAAGGCCTTCCGATGATC	151.30872
BB80316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85172	WGS84	BB80316	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80316_Upin_Bot2011_COI	GB13	1	BB80316_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80316	Sample	BB80316	targeted	Absent	Sample	Upin_Bot2011_COI	GB13	COI	45	BB80316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB13	CTTTAATTACAGCGTTTTTATTGTTGT	151.30872
BB80317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8531	WGS84	BB80317	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80317_Mgig_Bot2012_COI	GB12	1	BB80317_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80317	Sample	BB80317	targeted	Absent	Sample	Mgig_Bot2012_COI	GB12	COI	45	BB80317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB12	TCTTATTCGTTGGAGACTTTATAACCCT	151.30906
BB80318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85238	WGS84	BB80318	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80318_Cmae_Bot2011_COI	GB8	1	BB80318_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80318	Sample	BB80318	targeted	Absent	Sample	Cmae_Bot2011_COI	GB8	COI	45	BB80318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB8	ATGAACAGTCTATCCTCCTTTAG	151.31641
BB80318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85238	WGS84	BB80318	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80318_Msal_Bot2012_COI	GB8	1	BB80318_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80318	Sample	BB80318	targeted	Absent	Sample	Msal_Bot2012_COI	GB8	COI	45	BB80318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB8	GYTAGTTCCRATGATGTTAGCTG	151.31641
BB80320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.85326	WGS84	BB80320	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80320_Mgig_Bot2012_COI	GB6	1	BB80320_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80320	Sample	BB80320	targeted	Absent	Sample	Mgig_Bot2012_COI	GB6	COI	45	BB80320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB6	TCTTATTCGTTGGAGACTTTATAACCCT	151.31809
BB80323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8559	WGS84	BB80323	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80323_Aamu_Bax2006_COI	GB2	1	BB80323_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80323	Sample	BB80323	targeted	Absent	Sample	Aamu_Bax2006_COI	GB2	COI	45	BB80323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB2	GCACAACCGGGATCTTTACTTC	151.32319
BB80323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8559	WGS84	BB80323	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80323_Pcan_Bot2011_IGS	GB2	1	BB80323_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80323	Sample	BB80323	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB2	IGS	45	BB80323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB2	CGTAATCCTCAGTACTGGCTA	151.32319
BB80323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8559	WGS84	BB80323	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80323_Sspa_Oph2007_COI	GB2	1	BB80323_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80323	Sample	BB80323	targeted	Absent	Sample	Sspa_Oph2007_COI	GB2	COI	45	BB80323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB2	GAAGGCCTTCCGATGATC	151.32319
BB80323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10147	-23.8559	WGS84	BB80323	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80323_Upin_Bot2011_COI	GB2	1	BB80323_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80323	Sample	BB80323	targeted	Absent	Sample	Upin_Bot2011_COI	GB2	COI	45	BB80323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB2	CTTTAATTACAGCGTTTTTATTGTTGT	151.32319
BB80477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42858	WGS84	BB80477	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80477_Aamu_Bax2006_COI	BB45	1	BB80477_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80477	Sample	BB80477	targeted	Absent	Sample	Aamu_Bax2006_COI	BB45	COI	45	BB80477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB45	GCACAACCGGGATCTTTACTTC	153.12175
BB80477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42858	WGS84	BB80477	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80477_Pcan_Bot2011_IGS	BB45	1	BB80477_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80477	Sample	BB80477	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB45	IGS	45	BB80477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB45	CGTAATCCTCAGTACTGGCTA	153.12175
BB80477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42858	WGS84	BB80477	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80477_Sspa_Oph2007_COI	BB45	1	BB80477_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80477	Sample	BB80477	targeted	Absent	Sample	Sspa_Oph2007_COI	BB45	COI	45	BB80477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB45	GAAGGCCTTCCGATGATC	153.12175
BB80477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42858	WGS84	BB80477	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80477_Upin_Bot2011_COI	BB45	1	BB80477_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80477	Sample	BB80477	targeted	Absent	Sample	Upin_Bot2011_COI	BB45	COI	45	BB80477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB45	CTTTAATTACAGCGTTTTTATTGTTGT	153.12175
BB80478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40392	WGS84	BB80478	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80478_Mgig_Bot2012_COI	BB60	1	BB80478_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80478	Sample	BB80478	targeted	Absent	Sample	Mgig_Bot2012_COI	BB60	COI	45	BB80478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB60	TCTTATTCGTTGGAGACTTTATAACCCT	153.15318
BB80479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4006	WGS84	BB80479	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80479_Cmae_Bot2011_COI	BB25	1	BB80479_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80479	Sample	BB80479	targeted	Absent	Sample	Cmae_Bot2011_COI	BB25	COI	45	BB80479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB25	ATGAACAGTCTATCCTCCTTTAG	153.16275
BB80479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4006	WGS84	BB80479	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80479_Msal_Bot2012_COI	BB25	1	BB80479_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80479	Sample	BB80479	targeted	Absent	Sample	Msal_Bot2012_COI	BB25	COI	45	BB80479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB25	GYTAGTTCCRATGATGTTAGCTG	153.16275
BB80480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44671	WGS84	BB80480	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80480_Aamu_Bax2006_COI	BB40	1	BB80480_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80480	Sample	BB80480	targeted	Absent	Sample	Aamu_Bax2006_COI	BB40	COI	45	BB80480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB40	GCACAACCGGGATCTTTACTTC	153.07953
BB80480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44671	WGS84	BB80480	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80480_Pcan_Bot2011_IGS	BB40	1	BB80480_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80480	Sample	BB80480	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB40	IGS	45	BB80480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB40	CGTAATCCTCAGTACTGGCTA	153.07953
BB80480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44671	WGS84	BB80480	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80480_Sspa_Oph2007_COI	BB40	1	BB80480_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80480	Sample	BB80480	targeted	Absent	Sample	Sspa_Oph2007_COI	BB40	COI	45	BB80480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB40	GAAGGCCTTCCGATGATC	153.07953
BB80480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44671	WGS84	BB80480	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80480_Upin_Bot2011_COI	BB40	1	BB80480_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80480	Sample	BB80480	targeted	Absent	Sample	Upin_Bot2011_COI	BB40	COI	45	BB80480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB40	CTTTAATTACAGCGTTTTTATTGTTGT	153.07953
BB80481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.38453	WGS84	BB80481	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80481_Mgig_Bot2012_COI	BB20	1	BB80481_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80481	Sample	BB80481	targeted	Absent	Sample	Mgig_Bot2012_COI	BB20	COI	45	BB80481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB20	TCTTATTCGTTGGAGACTTTATAACCCT	153.15992
BB80482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37816	WGS84	BB80482	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80482_Cmae_Bot2011_COI	BB15	1	BB80482_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80482	Sample	BB80482	targeted	Absent	Sample	Cmae_Bot2011_COI	BB15	COI	45	BB80482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB15	ATGAACAGTCTATCCTCCTTTAG	153.16523
BB80482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.37816	WGS84	BB80482	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80482_Msal_Bot2012_COI	BB15	1	BB80482_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80482	Sample	BB80482	targeted	Absent	Sample	Msal_Bot2012_COI	BB15	COI	45	BB80482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB15	GYTAGTTCCRATGATGTTAGCTG	153.16523
BB80484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41854	WGS84	BB80484	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80484_Aamu_Bax2006_COI	BB56	1	BB80484_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80484	Sample	BB80484	targeted	Absent	Sample	Aamu_Bax2006_COI	BB56	COI	45	BB80484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB56	GCACAACCGGGATCTTTACTTC	153.13951
BB80484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41854	WGS84	BB80484	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80484_Pcan_Bot2011_IGS	BB56	1	BB80484_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80484	Sample	BB80484	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB56	IGS	45	BB80484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB56	CGTAATCCTCAGTACTGGCTA	153.13951
BB80484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41854	WGS84	BB80484	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80484_Sspa_Oph2007_COI	BB56	1	BB80484_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80484	Sample	BB80484	targeted	Absent	Sample	Sspa_Oph2007_COI	BB56	COI	45	BB80484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB56	GAAGGCCTTCCGATGATC	153.13951
BB80484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.41854	WGS84	BB80484	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80484_Upin_Bot2011_COI	BB56	1	BB80484_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80484	Sample	BB80484	targeted	Absent	Sample	Upin_Bot2011_COI	BB56	COI	45	BB80484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB56	CTTTAATTACAGCGTTTTTATTGTTGT	153.13951
BB80485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42242	WGS84	BB80485	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80485_Mgig_Bot2012_COI	BB54	1	BB80485_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80485	Sample	BB80485	targeted	Absent	Sample	Mgig_Bot2012_COI	BB54	COI	45	BB80485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB54	TCTTATTCGTTGGAGACTTTATAACCCT	153.13425
BB80486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42478	WGS84	BB80486	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80486_Cmae_Bot2011_COI	BB53	1	BB80486_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80486	Sample	BB80486	targeted	Absent	Sample	Cmae_Bot2011_COI	BB53	COI	45	BB80486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB53	ATGAACAGTCTATCCTCCTTTAG	153.12951
BB80486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.42478	WGS84	BB80486	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80486_Msal_Bot2012_COI	BB53	1	BB80486_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80486	Sample	BB80486	targeted	Absent	Sample	Msal_Bot2012_COI	BB53	COI	45	BB80486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB53	GYTAGTTCCRATGATGTTAGCTG	153.12951
BB80488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4305	WGS84	BB80488	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80488_Aamu_Bax2006_COI	BB47	1	BB80488_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80488	Sample	BB80488	targeted	Absent	Sample	Aamu_Bax2006_COI	BB47	COI	45	BB80488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB47	GCACAACCGGGATCTTTACTTC	153.12348
BB80488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4305	WGS84	BB80488	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80488_Pcan_Bot2011_IGS	BB47	1	BB80488_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80488	Sample	BB80488	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB47	IGS	45	BB80488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB47	CGTAATCCTCAGTACTGGCTA	153.12348
BB80488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4305	WGS84	BB80488	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80488_Sspa_Oph2007_COI	BB47	1	BB80488_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80488	Sample	BB80488	targeted	Absent	Sample	Sspa_Oph2007_COI	BB47	COI	45	BB80488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB47	GAAGGCCTTCCGATGATC	153.12348
BB80488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4305	WGS84	BB80488	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80488_Upin_Bot2011_COI	BB47	1	BB80488_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80488	Sample	BB80488	targeted	Absent	Sample	Upin_Bot2011_COI	BB47	COI	45	BB80488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB47	CTTTAATTACAGCGTTTTTATTGTTGT	153.12348
BB80489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.43049	WGS84	BB80489	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80489_Mgig_Bot2012_COI	BB44	1	BB80489_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80489	Sample	BB80489	targeted	Absent	Sample	Mgig_Bot2012_COI	BB44	COI	45	BB80489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB44	TCTTATTCGTTGGAGACTTTATAACCCT	153.11875
BB80491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4011	WGS84	BB80491	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80491_Aamu_Bax2006_COI	BB58	1	BB80491_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80491	Sample	BB80491	targeted	Absent	Sample	Aamu_Bax2006_COI	BB58	COI	45	BB80491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB58	GCACAACCGGGATCTTTACTTC	153.15819
BB80491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4011	WGS84	BB80491	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80491_Pcan_Bot2011_IGS	BB58	1	BB80491_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80491	Sample	BB80491	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB58	IGS	45	BB80491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB58	CGTAATCCTCAGTACTGGCTA	153.15819
BB80491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4011	WGS84	BB80491	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80491_Sspa_Oph2007_COI	BB58	1	BB80491_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80491	Sample	BB80491	targeted	Absent	Sample	Sspa_Oph2007_COI	BB58	COI	45	BB80491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB58	GAAGGCCTTCCGATGATC	153.15819
BB80491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.4011	WGS84	BB80491	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80491_Upin_Bot2011_COI	BB58	1	BB80491_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80491	Sample	BB80491	targeted	Absent	Sample	Upin_Bot2011_COI	BB58	COI	45	BB80491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB58	CTTTAATTACAGCGTTTTTATTGTTGT	153.15819
BB80492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.40135	WGS84	BB80492	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80492_Mgig_Bot2012_COI	BB27	1	BB80492_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80492	Sample	BB80492	targeted	Absent	Sample	Mgig_Bot2012_COI	BB27	COI	45	BB80492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB27	TCTTATTCGTTGGAGACTTTATAACCCT	153.16103
BB80493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44583	WGS84	BB80493	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80493_Cmae_Bot2011_COI	BB42	1	BB80493_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80493	Sample	BB80493	targeted	Absent	Sample	Cmae_Bot2011_COI	BB42	COI	45	BB80493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB42	ATGAACAGTCTATCCTCCTTTAG	153.08345
BB80493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44583	WGS84	BB80493	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80493_Msal_Bot2012_COI	BB42	1	BB80493_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80493	Sample	BB80493	targeted	Absent	Sample	Msal_Bot2012_COI	BB42	COI	45	BB80493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB42	GYTAGTTCCRATGATGTTAGCTG	153.08345
BB80495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44492	WGS84	BB80495	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80495_Aamu_Bax2006_COI	BB37	1	BB80495_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80495	Sample	BB80495	targeted	Absent	Sample	Aamu_Bax2006_COI	BB37	COI	45	BB80495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB37	GCACAACCGGGATCTTTACTTC	153.07984
BB80495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44492	WGS84	BB80495	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80495_Pcan_Bot2011_IGS	BB37	1	BB80495_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80495	Sample	BB80495	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB37	IGS	45	BB80495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB37	CGTAATCCTCAGTACTGGCTA	153.07984
BB80495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44492	WGS84	BB80495	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80495_Sspa_Oph2007_COI	BB37	1	BB80495_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80495	Sample	BB80495	targeted	Absent	Sample	Sspa_Oph2007_COI	BB37	COI	45	BB80495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB37	GAAGGCCTTCCGATGATC	153.07984
BB80495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44492	WGS84	BB80495	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80495_Upin_Bot2011_COI	BB37	1	BB80495_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80495	Sample	BB80495	targeted	Absent	Sample	Upin_Bot2011_COI	BB37	COI	45	BB80495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB37	CTTTAATTACAGCGTTTTTATTGTTGT	153.07984
BB80496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44558	WGS84	BB80496	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80496_Mgig_Bot2012_COI	BB36	1	BB80496_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80496	Sample	BB80496	targeted	Absent	Sample	Mgig_Bot2012_COI	BB36	COI	45	BB80496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB36	TCTTATTCGTTGGAGACTTTATAACCCT	153.07838
BB80497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44567	WGS84	BB80497	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80497_Cmae_Bot2011_COI	BB34	1	BB80497_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80497	Sample	BB80497	targeted	Absent	Sample	Cmae_Bot2011_COI	BB34	COI	45	BB80497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB34	ATGAACAGTCTATCCTCCTTTAG	153.07495
BB80497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44567	WGS84	BB80497	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80497_Msal_Bot2012_COI	BB34	1	BB80497_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80497	Sample	BB80497	targeted	Absent	Sample	Msal_Bot2012_COI	BB34	COI	45	BB80497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB34	GYTAGTTCCRATGATGTTAGCTG	153.07495
BB80499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44256	WGS84	BB80499	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80499_Aamu_Bax2006_COI	BB29	1	BB80499_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80499	Sample	BB80499	targeted	Absent	Sample	Aamu_Bax2006_COI	BB29	COI	45	BB80499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB29	GCACAACCGGGATCTTTACTTC	153.07224
BB80499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44256	WGS84	BB80499	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80499_Pcan_Bot2011_IGS	BB29	1	BB80499_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80499	Sample	BB80499	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB29	IGS	45	BB80499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB29	CGTAATCCTCAGTACTGGCTA	153.07224
BB80499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44256	WGS84	BB80499	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80499_Sspa_Oph2007_COI	BB29	1	BB80499_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80499	Sample	BB80499	targeted	Absent	Sample	Sspa_Oph2007_COI	BB29	COI	45	BB80499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB29	GAAGGCCTTCCGATGATC	153.07224
BB80499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.44256	WGS84	BB80499	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	16/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80499_Upin_Bot2011_COI	BB29	1	BB80499_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80499	Sample	BB80499	targeted	Absent	Sample	Upin_Bot2011_COI	BB29	COI	45	BB80499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB29	CTTTAATTACAGCGTTTTTATTGTTGT	153.07224
BB80500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.3698	WGS84	BB80500	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80500_Mgig_Bot2012_COI	BB9	1	BB80500_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80500	Sample	BB80500	targeted	Absent	Sample	Mgig_Bot2012_COI	BB9	COI	45	BB80500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB9	TCTTATTCGTTGGAGACTTTATAACCCT	153.17124
BB80501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36806	WGS84	BB80501	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB80501_Cmae_Bot2011_COI	BB8	1	BB80501_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80501	Sample	BB80501	targeted	Absent	Sample	Cmae_Bot2011_COI	BB8	COI	45	BB80501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB8	ATGAACAGTCTATCCTCCTTTAG	153.17231
BB80501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36806	WGS84	BB80501	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB80501_Msal_Bot2012_COI	BB8	1	BB80501_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80501	Sample	BB80501	targeted	Absent	Sample	Msal_Bot2012_COI	BB8	COI	45	BB80501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB8	GYTAGTTCCRATGATGTTAGCTG	153.17231
BB80503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36134	WGS84	BB80503	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB80503_Aamu_Bax2006_COI	BB4	1	BB80503_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80503	Sample	BB80503	targeted	Absent	Sample	Aamu_Bax2006_COI	BB4	COI	45	BB80503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB4	GCACAACCGGGATCTTTACTTC	153.17733
BB80503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36134	WGS84	BB80503	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB80503_Pcan_Bot2011_IGS	BB4	1	BB80503_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80503	Sample	BB80503	targeted	Absent	Sample	Pcan_Bot2011_IGS	BB4	IGS	45	BB80503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB4	CGTAATCCTCAGTACTGGCTA	153.17733
BB80503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36134	WGS84	BB80503	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB80503_Sspa_Oph2007_COI	BB4	1	BB80503_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80503	Sample	BB80503	targeted	Absent	Sample	Sspa_Oph2007_COI	BB4	COI	45	BB80503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB4	GAAGGCCTTCCGATGATC	153.17733
BB80503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.36134	WGS84	BB80503	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB80503_Upin_Bot2011_COI	BB4	1	BB80503_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80503	Sample	BB80503	targeted	Absent	Sample	Upin_Bot2011_COI	BB4	COI	45	BB80503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB4	CTTTAATTACAGCGTTTTTATTGTTGT	153.17733
BB80504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10148	-27.39048	WGS84	BB80504	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Spring	15/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB80504_Mgig_Bot2012_COI	BB24	1	BB80504_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB80504	Sample	BB80504	targeted	Absent	Sample	Mgig_Bot2012_COI	BB24	COI	45	BB80504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BB24	TCTTATTCGTTGGAGACTTTATAACCCT	153.15714
BB87784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85098	WGS84	BB87784	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87784_Aamu_Bax2006_COI	T11-02	1	BB87784_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87784	Sample	BB87784	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-02	COI	45	BB87784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-02	GCACAACCGGGATCTTTACTTC	147.87402
BB87784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85098	WGS84	BB87784	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87784_Pcan_Bot2011_IGS	T11-02	1	BB87784_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87784	Sample	BB87784	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-02	IGS	45	BB87784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-02	CGTAATCCTCAGTACTGGCTA	147.87402
BB87784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85098	WGS84	BB87784	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87784_Sspa_Oph2007_COI	T11-02	1	BB87784_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87784	Sample	BB87784	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-02	COI	45	BB87784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-02	GAAGGCCTTCCGATGATC	147.87402
BB87784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85098	WGS84	BB87784	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.096	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87784_Upin_Bot2011_COI	T11-02	1	BB87784_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87784	Sample	BB87784	targeted	Present	Sample	Upin_Bot2011_COI	T11-02	COI	45	BB87784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.87402
BB87785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85535	WGS84	BB87785	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87785_Cjap_Sim2018_COI	T11-03	1	BB87785_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87785	Sample	BB87785	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-03	COI	45	BB87785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87278
BB87785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85535	WGS84	BB87785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	27/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87785_Mgig_Bot2012_COI	T11-03	1	BB87785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87785	Sample	BB87785	targeted	Absent	Sample	Mgig_Bot2012_COI	T11-03	COI	45	BB87785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.87278
BB87786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88943	WGS84	BB87786	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	27/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87786_Cmae_Bot2011_COI	T11-04	1	BB87786_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87786	Sample	BB87786	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-04	COI	45	BB87786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-04	ATGAACAGTCTATCCTCCTTTAG	147.81458
BB87788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.95998	WGS84	BB87788	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.077	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87788_Aamu_Bax2006_COI	T11-06	1	BB87788_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87788	Sample	BB87788	targeted	Present	Sample	Aamu_Bax2006_COI	T11-06	COI	45	BB87788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-06	GCACAACCGGGATCTTTACTTC	147.52818
BB87788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.95998	WGS84	BB87788	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87788_Pcan_Bot2011_IGS	T11-06	1	BB87788_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87788	Sample	BB87788	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-06	IGS	45	BB87788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-06	CGTAATCCTCAGTACTGGCTA	147.52818
BB87788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.95998	WGS84	BB87788	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87788_Sspa_Oph2007_COI	T11-06	1	BB87788_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87788	Sample	BB87788	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-06	COI	45	BB87788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-06	GAAGGCCTTCCGATGATC	147.52818
BB87788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.95998	WGS84	BB87788	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87788_Upin_Bot2011_COI	T11-06	1	BB87788_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87788	Sample	BB87788	targeted	Absent	Sample	Upin_Bot2011_COI	T11-06	COI	45	BB87788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.52818
BB87789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96348	WGS84	BB87789	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	29/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87789_Cjap_Sim2018_COI	T11-07	1	BB87789_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87789	Sample	BB87789	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-07	COI	45	BB87789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51931
BB87789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96348	WGS84	BB87789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.802	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	29/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87789_Mgig_Bot2012_COI	T11-07	1	BB87789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87789	Sample	BB87789	targeted	Present	Sample	Mgig_Bot2012_COI	T11-07	COI	45	BB87789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.51931
BB87791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8275	WGS84	BB87791	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87791_Aamu_Bax2006_COI	T11-09	1	BB87791_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87791	Sample	BB87791	targeted	Absent	Sample	Aamu_Bax2006_COI	T11-09	COI	45	BB87791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-09	GCACAACCGGGATCTTTACTTC	147.30215
BB87791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8275	WGS84	BB87791	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87791_Pcan_Bot2011_IGS	T11-09	1	BB87791_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87791	Sample	BB87791	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-09	IGS	45	BB87791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-09	CGTAATCCTCAGTACTGGCTA	147.30215
BB87791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8275	WGS84	BB87791	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87791_Sspa_Oph2007_COI	T11-09	1	BB87791_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87791	Sample	BB87791	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-09	COI	45	BB87791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-09	GAAGGCCTTCCGATGATC	147.30215
BB87791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8275	WGS84	BB87791	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87791_Upin_Bot2011_COI	T11-09	1	BB87791_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87791	Sample	BB87791	targeted	Absent	Sample	Upin_Bot2011_COI	T11-09	COI	45	BB87791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.30215
BB87792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82887	WGS84	BB87792	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87792_Cjap_Sim2018_COI	T11-10	1	BB87792_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87792	Sample	BB87792	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-10	COI	45	BB87792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30192
BB87792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82887	WGS84	BB87792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.193	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87792_Mgig_Bot2012_COI	T11-10	1	BB87792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87792	Sample	BB87792	targeted	Present	Sample	Mgig_Bot2012_COI	T11-10	COI	45	BB87792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.30192
BB87793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87793	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87793_Cmae_Bot2011_COI	T11-11	1	BB87793_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87793	Sample	BB87793	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-11	COI	45	BB87793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-11	ATGAACAGTCTATCCTCCTTTAG	147.33688
BB87795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88453	WGS84	BB87795	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.276	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87795_Aamu_Bax2006_COI	T11-12	1	BB87795_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87795	Sample	BB87795	targeted	Present	Sample	Aamu_Bax2006_COI	T11-12	COI	45	BB87795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-12	GCACAACCGGGATCTTTACTTC	147.33611
BB87795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88453	WGS84	BB87795	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87795_Pcan_Bot2011_IGS	T11-12	1	BB87795_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87795	Sample	BB87795	targeted	Absent	Sample	Pcan_Bot2011_IGS	T11-12	IGS	45	BB87795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-12	CGTAATCCTCAGTACTGGCTA	147.33611
BB87795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88453	WGS84	BB87795	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87795_Sspa_Oph2007_COI	T11-12	1	BB87795_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87795	Sample	BB87795	targeted	Absent	Sample	Sspa_Oph2007_COI	T11-12	COI	45	BB87795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-12	GAAGGCCTTCCGATGATC	147.33611
BB87795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88453	WGS84	BB87795	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87795_Upin_Bot2011_COI	T11-12	1	BB87795_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87795	Sample	BB87795	targeted	Absent	Sample	Upin_Bot2011_COI	T11-12	COI	45	BB87795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.33611
BB87796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88082	WGS84	BB87796	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87796_Cjap_Sim2018_COI	T11-14	1	BB87796_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87796	Sample	BB87796	targeted	Absent	Sample	Cjap_Sim2018_COI	T11-14	COI	45	BB87796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3422
BB87796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88082	WGS84	BB87796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.014	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T11	30/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87796_Mgig_Bot2012_COI	T11-14	1	BB87796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87796	Sample	BB87796	targeted	Present	Sample	Mgig_Bot2012_COI	T11-14	COI	45	BB87796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.3422
BB87797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87836	WGS84	BB87797	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T11	30/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87797_Cmae_Bot2011_COI	T11-15	1	BB87797_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87797	Sample	BB87797	targeted	Absent	Sample	Cmae_Bot2011_COI	T11-15	COI	45	BB87797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T11-15	ATGAACAGTCTATCCTCCTTTAG	147.34177
BB87799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84645	WGS84	BB87799	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87799_Aamu_Bax2006_COI	S7-02	1	BB87799_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87799	Sample	BB87799	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-02	COI	45	BB87799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-02	GCACAACCGGGATCTTTACTTC	138.49705
BB87799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84645	WGS84	BB87799	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87799_Pcan_Bot2011_IGS	S7-02	1	BB87799_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87799	Sample	BB87799	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-02	IGS	45	BB87799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-02	CGTAATCCTCAGTACTGGCTA	138.49705
BB87799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84645	WGS84	BB87799	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87799_Sspa_Oph2007_COI	S7-02	1	BB87799_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87799	Sample	BB87799	targeted	Absent	Sample	Sspa_Oph2007_COI	S7-02	COI	45	BB87799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-02	GAAGGCCTTCCGATGATC	138.49705
BB87799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84645	WGS84	BB87799	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87799_Upin_Bot2011_COI	S7-02	1	BB87799_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87799	Sample	BB87799	targeted	Absent	Sample	Upin_Bot2011_COI	S7-02	COI	45	BB87799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.49705
BB87800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84557	WGS84	BB87800	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87800_Cjap_Sim2018_COI	S7-03	1	BB87800_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87800	Sample	BB87800	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-03	COI	45	BB87800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49696
BB87800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84557	WGS84	BB87800	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.084	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87800_Mgig_Bot2012_COI	S7-03	1	BB87800_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87800	Sample	BB87800	targeted	Present	Sample	Mgig_Bot2012_COI	S7-03	COI	45	BB87800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.49696
BB87803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83649	WGS84	BB87803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87803_Aamu_Bax2006_COI	S7-06	1	BB87803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87803	Sample	BB87803	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-06	COI	45	BB87803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-06	GCACAACCGGGATCTTTACTTC	138.50852
BB87803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83649	WGS84	BB87803	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87803_Pcan_Bot2011_IGS	S7-06	1	BB87803_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87803	Sample	BB87803	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-06	IGS	45	BB87803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-06	CGTAATCCTCAGTACTGGCTA	138.50852
BB87803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83649	WGS84	BB87803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87803_Sspa_Oph2007_COI	S7-06	1	BB87803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87803	Sample	BB87803	targeted	Absent	Sample	Sspa_Oph2007_COI	S7-06	COI	45	BB87803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-06	GAAGGCCTTCCGATGATC	138.50852
BB87803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83649	WGS84	BB87803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87803_Upin_Bot2011_COI	S7-06	1	BB87803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87803	Sample	BB87803	targeted	Absent	Sample	Upin_Bot2011_COI	S7-06	COI	45	BB87803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.50852
BB87804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83704	WGS84	BB87804	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87804_Cjap_Sim2018_COI	S7-07	1	BB87804_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87804	Sample	BB87804	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-07	COI	45	BB87804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50986
BB87804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83704	WGS84	BB87804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87804_Mgig_Bot2012_COI	S7-07	1	BB87804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87804	Sample	BB87804	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-07	COI	45	BB87804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.50986
BB87805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.605	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87805_Cmae_Bot2011_COI	S7-08	1	BB87805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87805	Sample	BB87805	targeted	Present	Sample	Cmae_Bot2011_COI	S7-08	COI	45	BB87805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-08	ATGAACAGTCTATCCTCCTTTAG	138.51031
BB87808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78381	WGS84	BB87808	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87808_Cjap_Sim2018_COI	S7-11	1	BB87808_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87808	Sample	BB87808	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-11	COI	45	BB87808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51578
BB87808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78381	WGS84	BB87808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87808_Mgig_Bot2012_COI	S7-11	1	BB87808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87808	Sample	BB87808	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-11	COI	45	BB87808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51578
BB87809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78326	WGS84	BB87809	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.598	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87809_Cmae_Bot2011_COI	S7-12	1	BB87809_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87809	Sample	BB87809	targeted	Present	Sample	Cmae_Bot2011_COI	S7-12	COI	45	BB87809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-12	ATGAACAGTCTATCCTCCTTTAG	138.51601
BB87810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78245	WGS84	BB87810	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87810_Aamu_Bax2006_COI	S7-13	1	BB87810_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87810	Sample	BB87810	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-13	COI	45	BB87810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-13	GCACAACCGGGATCTTTACTTC	138.51632
BB87810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78245	WGS84	BB87810	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87810_Pcan_Bot2011_IGS	S7-13	1	BB87810_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87810	Sample	BB87810	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-13	IGS	45	BB87810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-13	CGTAATCCTCAGTACTGGCTA	138.51632
BB87810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78245	WGS84	BB87810	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.573	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87810_Sspa_Oph2007_COI	S7-13	1	BB87810_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87810	Sample	BB87810	targeted	Present	Sample	Sspa_Oph2007_COI	S7-13	COI	45	BB87810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-13	GAAGGCCTTCCGATGATC	138.51632
BB87810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78245	WGS84	BB87810	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87810_Upin_Bot2011_COI	S7-13	1	BB87810_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87810	Sample	BB87810	targeted	Absent	Sample	Upin_Bot2011_COI	S7-13	COI	45	BB87810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.51632
BB87814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77378	WGS84	BB87814	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87814_Aamu_Bax2006_COI	S7-17	1	BB87814_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87814	Sample	BB87814	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-17	COI	45	BB87814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-17	GCACAACCGGGATCTTTACTTC	138.4857
BB87814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77378	WGS84	BB87814	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87814_Pcan_Bot2011_IGS	S7-17	1	BB87814_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87814	Sample	BB87814	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-17	IGS	45	BB87814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-17	CGTAATCCTCAGTACTGGCTA	138.4857
BB87814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77378	WGS84	BB87814	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.339	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87814_Sspa_Oph2007_COI	S7-17	1	BB87814_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87814	Sample	BB87814	targeted	Present	Sample	Sspa_Oph2007_COI	S7-17	COI	45	BB87814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-17	GAAGGCCTTCCGATGATC	138.4857
BB87814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77378	WGS84	BB87814	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87814_Upin_Bot2011_COI	S7-17	1	BB87814_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87814	Sample	BB87814	targeted	Absent	Sample	Upin_Bot2011_COI	S7-17	COI	45	BB87814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.4857
BB87815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77396	WGS84	BB87815	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87815_Cjap_Sim2018_COI	S7-18	1	BB87815_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87815	Sample	BB87815	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-18	COI	45	BB87815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.486
BB87815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77396	WGS84	BB87815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87815_Mgig_Bot2012_COI	S7-18	1	BB87815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87815	Sample	BB87815	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-18	COI	45	BB87815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.486
BB87818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80928	WGS84	BB87818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87818_Aamu_Bax2006_COI	S7-21	1	BB87818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87818	Sample	BB87818	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-21	COI	45	BB87818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-21	GCACAACCGGGATCTTTACTTC	138.51791
BB87818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80928	WGS84	BB87818	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87818_Pcan_Bot2011_IGS	S7-21	1	BB87818_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87818	Sample	BB87818	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-21	IGS	45	BB87818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-21	CGTAATCCTCAGTACTGGCTA	138.51791
BB87818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80928	WGS84	BB87818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.37	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87818_Sspa_Oph2007_COI	S7-21	1	BB87818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87818	Sample	BB87818	targeted	Present	Sample	Sspa_Oph2007_COI	S7-21	COI	45	BB87818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-21	GAAGGCCTTCCGATGATC	138.51791
BB87818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80928	WGS84	BB87818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87818_Upin_Bot2011_COI	S7-21	1	BB87818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87818	Sample	BB87818	targeted	Absent	Sample	Upin_Bot2011_COI	S7-21	COI	45	BB87818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-21	CTTTAATTACAGCGTTTTTATTGTTGT	138.51791
BB87819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80999	WGS84	BB87819	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87819_Cjap_Sim2018_COI	S7-22	1	BB87819_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87819	Sample	BB87819	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-22	COI	45	BB87819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-22	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51976
BB87819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.80999	WGS84	BB87819	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.732	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87819_Mgig_Bot2012_COI	S7-22	1	BB87819_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87819	Sample	BB87819	targeted	Present	Sample	Mgig_Bot2012_COI	S7-22	COI	45	BB87819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-22	TCTTATTCGTTGGAGACTTTATAACCCT	138.51976
BB87822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81158	WGS84	BB87822	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87822_Cjap_Sim2018_COI	S7-25	1	BB87822_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87822	Sample	BB87822	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-25	COI	45	BB87822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-25	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52358
BB87822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.81158	WGS84	BB87822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.406	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87822_Mgig_Bot2012_COI	S7-25	1	BB87822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87822	Sample	BB87822	targeted	Present	Sample	Mgig_Bot2012_COI	S7-25	COI	45	BB87822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-25	TCTTATTCGTTGGAGACTTTATAACCCT	138.52358
BB87823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84719	WGS84	BB87823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.24	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87823_Cmae_Bot2011_COI	S7-01	1	BB87823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87823	Sample	BB87823	targeted	Present	Sample	Cmae_Bot2011_COI	S7-01	COI	45	BB87823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-01	ATGAACAGTCTATCCTCCTTTAG	138.4975
BB87825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85088	WGS84	BB87825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87825_Aamu_Bax2006_COI	T12-02	1	BB87825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87825	Sample	BB87825	targeted	Absent	Sample	Aamu_Bax2006_COI	T12-02	COI	45	BB87825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-02	GCACAACCGGGATCTTTACTTC	147.87377
BB87825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85088	WGS84	BB87825	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87825_Pcan_Bot2011_IGS	T12-02	1	BB87825_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87825	Sample	BB87825	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-02	IGS	45	BB87825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-02	CGTAATCCTCAGTACTGGCTA	147.87377
BB87825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85088	WGS84	BB87825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87825_Sspa_Oph2007_COI	T12-02	1	BB87825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87825	Sample	BB87825	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-02	COI	45	BB87825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-02	GAAGGCCTTCCGATGATC	147.87377
BB87825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85088	WGS84	BB87825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87825_Upin_Bot2011_COI	T12-02	1	BB87825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87825	Sample	BB87825	targeted	Absent	Sample	Upin_Bot2011_COI	T12-02	COI	45	BB87825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.87377
BB87826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85596	WGS84	BB87826	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87826_Cjap_Sim2018_COI	T12-03	1	BB87826_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87826	Sample	BB87826	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-03	COI	45	BB87826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87236
BB87826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85596	WGS84	BB87826	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	28/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87826_Mgig_Bot2012_COI	T12-03	1	BB87826_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87826	Sample	BB87826	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-03	COI	45	BB87826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.87236
BB87827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88956	WGS84	BB87827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	28/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87827_Cmae_Bot2011_COI	T12-04	1	BB87827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87827	Sample	BB87827	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-04	COI	45	BB87827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-04	ATGAACAGTCTATCCTCCTTTAG	147.81287
BB87829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87783	WGS84	BB87829	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.785	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87829_Aamu_Bax2006_COI	T12-06	1	BB87829_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87829	Sample	BB87829	targeted	Present	Sample	Aamu_Bax2006_COI	T12-06	COI	45	BB87829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-06	GCACAACCGGGATCTTTACTTC	147.34193
BB87829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87783	WGS84	BB87829	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87829_Pcan_Bot2011_IGS	T12-06	1	BB87829_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87829	Sample	BB87829	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-06	IGS	45	BB87829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-06	CGTAATCCTCAGTACTGGCTA	147.34193
BB87829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87783	WGS84	BB87829	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87829_Sspa_Oph2007_COI	T12-06	1	BB87829_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87829	Sample	BB87829	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-06	COI	45	BB87829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-06	GAAGGCCTTCCGATGATC	147.34193
BB87829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87783	WGS84	BB87829	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87829_Upin_Bot2011_COI	T12-06	1	BB87829_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87829	Sample	BB87829	targeted	Absent	Sample	Upin_Bot2011_COI	T12-06	COI	45	BB87829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.34193
BB87830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87348	WGS84	BB87830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87830_Cmae_Bot2011_COI	T12-07	1	BB87830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87830	Sample	BB87830	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-07	COI	45	BB87830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-07	ATGAACAGTCTATCCTCCTTTAG	147.33661
BB87832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82729	WGS84	BB87832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.613	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87832_Aamu_Bax2006_COI	T12-09	1	BB87832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87832	Sample	BB87832	targeted	Present	Sample	Aamu_Bax2006_COI	T12-09	COI	45	BB87832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-09	GCACAACCGGGATCTTTACTTC	147.30174
BB87832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82729	WGS84	BB87832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87832_Pcan_Bot2011_IGS	T12-09	1	BB87832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87832	Sample	BB87832	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-09	IGS	45	BB87832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-09	CGTAATCCTCAGTACTGGCTA	147.30174
BB87832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82729	WGS84	BB87832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87832_Sspa_Oph2007_COI	T12-09	1	BB87832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87832	Sample	BB87832	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-09	COI	45	BB87832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-09	GAAGGCCTTCCGATGATC	147.30174
BB87832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82729	WGS84	BB87832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87832_Upin_Bot2011_COI	T12-09	1	BB87832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87832	Sample	BB87832	targeted	Absent	Sample	Upin_Bot2011_COI	T12-09	COI	45	BB87832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.30174
BB87833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82518	WGS84	BB87833	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87833_Cjap_Sim2018_COI	T12-10	1	BB87833_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87833	Sample	BB87833	targeted	Absent	Sample	Cjap_Sim2018_COI	T12-10	COI	45	BB87833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30494
BB87833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82518	WGS84	BB87833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T12	29/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87833_Mgig_Bot2012_COI	T12-10	1	BB87833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87833	Sample	BB87833	targeted	Absent	Sample	Mgig_Bot2012_COI	T12-10	COI	45	BB87833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.30494
BB87834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88553	WGS84	BB87834	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87834_Cmae_Bot2011_COI	T12-11	1	BB87834_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87834	Sample	BB87834	targeted	Absent	Sample	Cmae_Bot2011_COI	T12-11	COI	45	BB87834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-11	ATGAACAGTCTATCCTCCTTTAG	147.33569
BB87836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88287	WGS84	BB87836	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.775	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87836_Aamu_Bax2006_COI	T12-13	1	BB87836_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87836	Sample	BB87836	targeted	Present	Sample	Aamu_Bax2006_COI	T12-13	COI	45	BB87836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-13	GCACAACCGGGATCTTTACTTC	147.33643
BB87836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88287	WGS84	BB87836	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87836_Pcan_Bot2011_IGS	T12-13	1	BB87836_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87836	Sample	BB87836	targeted	Absent	Sample	Pcan_Bot2011_IGS	T12-13	IGS	45	BB87836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-13	CGTAATCCTCAGTACTGGCTA	147.33643
BB87836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88287	WGS84	BB87836	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87836_Sspa_Oph2007_COI	T12-13	1	BB87836_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87836	Sample	BB87836	targeted	Absent	Sample	Sspa_Oph2007_COI	T12-13	COI	45	BB87836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-13	GAAGGCCTTCCGATGATC	147.33643
BB87836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88287	WGS84	BB87836	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T12	29/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87836_Upin_Bot2011_COI	T12-13	1	BB87836_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87836	Sample	BB87836	targeted	Absent	Sample	Upin_Bot2011_COI	T12-13	COI	45	BB87836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T12-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.33643
BB87837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83653	WGS84	BB87837	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87837_Cjap_Sim2018_COI	S8-25	1	BB87837_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87837	Sample	BB87837	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-25	COI	45	BB87837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-25	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50737
BB87837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83653	WGS84	BB87837	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.978	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87837_Mgig_Bot2012_COI	S8-25	1	BB87837_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87837	Sample	BB87837	targeted	Present	Sample	Mgig_Bot2012_COI	S8-25	COI	45	BB87837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-25	TCTTATTCGTTGGAGACTTTATAACCCT	138.50737
BB87840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96205	WGS84	BB87840	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87840_Cjap_Sim2018_COI	T13-01	1	BB87840_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87840	Sample	BB87840	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-01	COI	45	BB87840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53986
BB87840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96205	WGS84	BB87840	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	40.583	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87840_Mgig_Bot2012_COI	T13-01	1	BB87840_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87840	Sample	BB87840	targeted	Present	Sample	Mgig_Bot2012_COI	T13-01	COI	45	BB87840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.53986
BB87841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96032	WGS84	BB87841	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87841_Cmae_Bot2011_COI	T13-02	1	BB87841_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87841	Sample	BB87841	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-02	COI	45	BB87841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-02	ATGAACAGTCTATCCTCCTTTAG	147.53078
BB87843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84846	WGS84	BB87843	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87843_Aamu_Bax2006_COI	T13-04	1	BB87843_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87843	Sample	BB87843	targeted	Absent	Sample	Aamu_Bax2006_COI	T13-04	COI	45	BB87843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-04	GCACAACCGGGATCTTTACTTC	147.87427
BB87843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84846	WGS84	BB87843	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87843_Pcan_Bot2011_IGS	T13-04	1	BB87843_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87843	Sample	BB87843	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-04	IGS	45	BB87843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-04	CGTAATCCTCAGTACTGGCTA	147.87427
BB87843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84846	WGS84	BB87843	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87843_Sspa_Oph2007_COI	T13-04	1	BB87843_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87843	Sample	BB87843	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-04	COI	45	BB87843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-04	GAAGGCCTTCCGATGATC	147.87427
BB87843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84846	WGS84	BB87843	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	42.288	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87843_Upin_Bot2011_COI	T13-04	1	BB87843_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87843	Sample	BB87843	targeted	Present	Sample	Upin_Bot2011_COI	T13-04	COI	45	BB87843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.87427
BB87844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85093	WGS84	BB87844	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87844_Cjap_Sim2018_COI	T13-05	1	BB87844_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87844	Sample	BB87844	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-05	COI	45	BB87844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87402
BB87844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85093	WGS84	BB87844	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87844_Mgig_Bot2012_COI	T13-05	1	BB87844_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87844	Sample	BB87844	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-05	COI	45	BB87844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.87402
BB87845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85552	WGS84	BB87845	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87845_Cmae_Bot2011_COI	T13-06	1	BB87845_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87845	Sample	BB87845	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-06	COI	45	BB87845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-06	ATGAACAGTCTATCCTCCTTTAG	147.87256
BB87847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8289	WGS84	BB87847	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87847_Aamu_Bax2006_COI	T13-08	1	BB87847_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87847	Sample	BB87847	targeted	Absent	Sample	Aamu_Bax2006_COI	T13-08	COI	45	BB87847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-08	GCACAACCGGGATCTTTACTTC	147.30228
BB87847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8289	WGS84	BB87847	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87847_Pcan_Bot2011_IGS	T13-08	1	BB87847_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87847	Sample	BB87847	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-08	IGS	45	BB87847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-08	CGTAATCCTCAGTACTGGCTA	147.30228
BB87847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8289	WGS84	BB87847	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87847_Sspa_Oph2007_COI	T13-08	1	BB87847_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87847	Sample	BB87847	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-08	COI	45	BB87847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-08	GAAGGCCTTCCGATGATC	147.30228
BB87847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8289	WGS84	BB87847	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87847_Upin_Bot2011_COI	T13-08	1	BB87847_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87847	Sample	BB87847	targeted	Absent	Sample	Upin_Bot2011_COI	T13-08	COI	45	BB87847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.30228
BB87848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82819	WGS84	BB87848	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87848_Cjap_Sim2018_COI	T13-09	1	BB87848_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87848	Sample	BB87848	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-09	COI	45	BB87848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30194
BB87848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82819	WGS84	BB87848	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87848_Mgig_Bot2012_COI	T13-09	1	BB87848_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87848	Sample	BB87848	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-09	COI	45	BB87848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.30194
BB87849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82561	WGS84	BB87849	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87849_Cmae_Bot2011_COI	T13-10	1	BB87849_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87849	Sample	BB87849	targeted	Absent	Sample	Cmae_Bot2011_COI	T13-10	COI	45	BB87849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-10	ATGAACAGTCTATCCTCCTTTAG	147.30381
BB87850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87321	WGS84	BB87850	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.841	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87850_Aamu_Bax2006_COI	T13-11	1	BB87850_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87850	Sample	BB87850	targeted	Present	Sample	Aamu_Bax2006_COI	T13-11	COI	45	BB87850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-11	GCACAACCGGGATCTTTACTTC	147.33664
BB87850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87321	WGS84	BB87850	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87850_Pcan_Bot2011_IGS	T13-11	1	BB87850_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87850	Sample	BB87850	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-11	IGS	45	BB87850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-11	CGTAATCCTCAGTACTGGCTA	147.33664
BB87850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87321	WGS84	BB87850	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87850_Sspa_Oph2007_COI	T13-11	1	BB87850_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87850	Sample	BB87850	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-11	COI	45	BB87850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-11	GAAGGCCTTCCGATGATC	147.33664
BB87850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87321	WGS84	BB87850	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87850_Upin_Bot2011_COI	T13-11	1	BB87850_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87850	Sample	BB87850	targeted	Absent	Sample	Upin_Bot2011_COI	T13-11	COI	45	BB87850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33664
BB87851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88126	WGS84	BB87851	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87851_Cjap_Sim2018_COI	T13-12	1	BB87851_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87851	Sample	BB87851	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-12	COI	45	BB87851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34203
BB87851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88126	WGS84	BB87851	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87851_Mgig_Bot2012_COI	T13-12	1	BB87851_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87851	Sample	BB87851	targeted	Absent	Sample	Mgig_Bot2012_COI	T13-12	COI	45	BB87851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.34203
BB87852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87988	WGS84	BB87852	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.093	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87852_Cmae_Bot2011_COI	T13-13	1	BB87852_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87852	Sample	BB87852	targeted	Present	Sample	Cmae_Bot2011_COI	T13-13	COI	45	BB87852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-13	ATGAACAGTCTATCCTCCTTTAG	147.3422
BB87854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88452	WGS84	BB87854	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.275	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87854_Aamu_Bax2006_COI	T13-15	1	BB87854_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87854	Sample	BB87854	targeted	Present	Sample	Aamu_Bax2006_COI	T13-15	COI	45	BB87854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-15	GCACAACCGGGATCTTTACTTC	147.33658
BB87854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88452	WGS84	BB87854	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87854_Pcan_Bot2011_IGS	T13-15	1	BB87854_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87854	Sample	BB87854	targeted	Absent	Sample	Pcan_Bot2011_IGS	T13-15	IGS	45	BB87854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-15	CGTAATCCTCAGTACTGGCTA	147.33658
BB87854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88452	WGS84	BB87854	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87854_Sspa_Oph2007_COI	T13-15	1	BB87854_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87854	Sample	BB87854	targeted	Absent	Sample	Sspa_Oph2007_COI	T13-15	COI	45	BB87854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-15	GAAGGCCTTCCGATGATC	147.33658
BB87854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88452	WGS84	BB87854	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87854_Upin_Bot2011_COI	T13-15	1	BB87854_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87854	Sample	BB87854	targeted	Absent	Sample	Upin_Bot2011_COI	T13-15	COI	45	BB87854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.33658
BB87855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88312	WGS84	BB87855	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T13	27/06/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87855_Cjap_Sim2018_COI	T13-16	1	BB87855_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87855	Sample	BB87855	targeted	Absent	Sample	Cjap_Sim2018_COI	T13-16	COI	45	BB87855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33675
BB87855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88312	WGS84	BB87855	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	40.513	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T13	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87855_Mgig_Bot2012_COI	T13-16	1	BB87855_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87855	Sample	BB87855	targeted	Present	Sample	Mgig_Bot2012_COI	T13-16	COI	45	BB87855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T13-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.33675
BB87856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84432	WGS84	BB87856	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87856_Cmae_Bot2011_COI	S8-16	1	BB87856_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87856	Sample	BB87856	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-16	COI	45	BB87856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-16	ATGAACAGTCTATCCTCCTTTAG	138.49778
BB87859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77317	WGS84	BB87859	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87859_Cjap_Sim2018_COI	S8-13	1	BB87859_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87859	Sample	BB87859	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-13	COI	45	BB87859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48449
BB87859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77317	WGS84	BB87859	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87859_Mgig_Bot2012_COI	S8-13	1	BB87859_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87859	Sample	BB87859	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-13	COI	45	BB87859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.48449
BB87861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77196	WGS84	BB87861	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87861_Aamu_Bax2006_COI	S8-11	1	BB87861_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87861	Sample	BB87861	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-11	COI	45	BB87861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-11	GCACAACCGGGATCTTTACTTC	138.48602
BB87861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77196	WGS84	BB87861	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87861_Pcan_Bot2011_IGS	S8-11	1	BB87861_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87861	Sample	BB87861	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-11	IGS	45	BB87861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-11	CGTAATCCTCAGTACTGGCTA	138.48602
BB87861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77196	WGS84	BB87861	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.153	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87861_Sspa_Oph2007_COI	S8-11	1	BB87861_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87861	Sample	BB87861	targeted	Present	Sample	Sspa_Oph2007_COI	S8-11	COI	45	BB87861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-11	GAAGGCCTTCCGATGATC	138.48602
BB87861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77196	WGS84	BB87861	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87861_Upin_Bot2011_COI	S8-11	1	BB87861_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87861	Sample	BB87861	targeted	Absent	Sample	Upin_Bot2011_COI	S8-11	COI	45	BB87861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.48602
BB87863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83652	WGS84	BB87863	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87863_Cmae_Bot2011_COI	S8-21	1	BB87863_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87863	Sample	BB87863	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-21	COI	45	BB87863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-21	ATGAACAGTCTATCCTCCTTTAG	138.50853
BB87865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78822	WGS84	BB87865	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87865_Aamu_Bax2006_COI	S8-07	1	BB87865_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87865	Sample	BB87865	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-07	COI	45	BB87865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-07	GCACAACCGGGATCTTTACTTC	138.51314
BB87865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78822	WGS84	BB87865	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87865_Pcan_Bot2011_IGS	S8-07	1	BB87865_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87865	Sample	BB87865	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-07	IGS	45	BB87865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-07	CGTAATCCTCAGTACTGGCTA	138.51314
BB87865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78822	WGS84	BB87865	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.733	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87865_Sspa_Oph2007_COI	S8-07	1	BB87865_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87865	Sample	BB87865	targeted	Present	Sample	Sspa_Oph2007_COI	S8-07	COI	45	BB87865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-07	GAAGGCCTTCCGATGATC	138.51314
BB87865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78822	WGS84	BB87865	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87865_Upin_Bot2011_COI	S8-07	1	BB87865_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87865	Sample	BB87865	targeted	Absent	Sample	Upin_Bot2011_COI	S8-07	COI	45	BB87865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.51314
BB87866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78942	WGS84	BB87866	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87866_Cjap_Sim2018_COI	S8-06	1	BB87866_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87866	Sample	BB87866	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-06	COI	45	BB87866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51307
BB87866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78942	WGS84	BB87866	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87866_Mgig_Bot2012_COI	S8-06	1	BB87866_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87866	Sample	BB87866	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-06	COI	45	BB87866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.51307
BB87867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84712	WGS84	BB87867	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87867_Cmae_Bot2011_COI	S8-18	1	BB87867_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87867	Sample	BB87867	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-18	COI	45	BB87867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-18	ATGAACAGTCTATCCTCCTTTAG	138.4976
BB87869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80964	WGS84	BB87869	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87869_Aamu_Bax2006_COI	S8-03	1	BB87869_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87869	Sample	BB87869	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-03	COI	45	BB87869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-03	GCACAACCGGGATCTTTACTTC	138.52021
BB87869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80964	WGS84	BB87869	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87869_Pcan_Bot2011_IGS	S8-03	1	BB87869_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87869	Sample	BB87869	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-03	IGS	45	BB87869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-03	CGTAATCCTCAGTACTGGCTA	138.52021
BB87869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80964	WGS84	BB87869	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.332	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87869_Sspa_Oph2007_COI	S8-03	1	BB87869_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87869	Sample	BB87869	targeted	Present	Sample	Sspa_Oph2007_COI	S8-03	COI	45	BB87869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-03	GAAGGCCTTCCGATGATC	138.52021
BB87869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80964	WGS84	BB87869	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87869_Upin_Bot2011_COI	S8-03	1	BB87869_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87869	Sample	BB87869	targeted	Absent	Sample	Upin_Bot2011_COI	S8-03	COI	45	BB87869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.52021
BB87870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80894	WGS84	BB87870	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.783	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87870_Cmae_Bot2011_COI	S8-02	1	BB87870_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87870	Sample	BB87870	targeted	Present	Sample	Cmae_Bot2011_COI	S8-02	COI	45	BB87870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-02	ATGAACAGTCTATCCTCCTTTAG	138.51889
BB87872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81034	WGS84	BB87872	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87872_Aamu_Bax2006_COI	S6-25	1	BB87872_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87872	Sample	BB87872	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-25	COI	45	BB87872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-25	GCACAACCGGGATCTTTACTTC	138.52098
BB87872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81034	WGS84	BB87872	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87872_Pcan_Bot2011_IGS	S6-25	1	BB87872_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87872	Sample	BB87872	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-25	IGS	45	BB87872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-25	CGTAATCCTCAGTACTGGCTA	138.52098
BB87872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81034	WGS84	BB87872	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.667	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87872_Sspa_Oph2007_COI	S6-25	1	BB87872_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87872	Sample	BB87872	targeted	Present	Sample	Sspa_Oph2007_COI	S6-25	COI	45	BB87872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-25	GAAGGCCTTCCGATGATC	138.52098
BB87872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81034	WGS84	BB87872	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87872_Upin_Bot2011_COI	S6-25	1	BB87872_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87872	Sample	BB87872	targeted	Absent	Sample	Upin_Bot2011_COI	S6-25	COI	45	BB87872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-25	CTTTAATTACAGCGTTTTTATTGTTGT	138.52098
BB87876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81155	WGS84	BB87876	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87876_Aamu_Bax2006_COI	S6-21	1	BB87876_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87876	Sample	BB87876	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-21	COI	45	BB87876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-21	GCACAACCGGGATCTTTACTTC	138.52436
BB87876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81155	WGS84	BB87876	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87876_Pcan_Bot2011_IGS	S6-21	1	BB87876_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87876	Sample	BB87876	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-21	IGS	45	BB87876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-21	CGTAATCCTCAGTACTGGCTA	138.52436
BB87876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81155	WGS84	BB87876	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87876_Sspa_Oph2007_COI	S6-21	1	BB87876_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87876	Sample	BB87876	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-21	COI	45	BB87876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-21	GAAGGCCTTCCGATGATC	138.52436
BB87876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81155	WGS84	BB87876	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87876_Upin_Bot2011_COI	S6-21	1	BB87876_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87876	Sample	BB87876	targeted	Absent	Sample	Upin_Bot2011_COI	S6-21	COI	45	BB87876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-21	CTTTAATTACAGCGTTTTTATTGTTGT	138.52436
BB87880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77382	WGS84	BB87880	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87880_Cjap_Sim2018_COI	S6-17	1	BB87880_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87880	Sample	BB87880	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-17	COI	45	BB87880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48565
BB87880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77382	WGS84	BB87880	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87880_Mgig_Bot2012_COI	S6-17	1	BB87880_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87880	Sample	BB87880	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-17	COI	45	BB87880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-17	TCTTATTCGTTGGAGACTTTATAACCCT	138.48565
BB87881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77307	WGS84	BB87881	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87881_Cmae_Bot2011_COI	S6-16	1	BB87881_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87881	Sample	BB87881	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-16	COI	45	BB87881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-16	ATGAACAGTCTATCCTCCTTTAG	138.48439
BB87884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78507	WGS84	BB87884	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87884_Cjap_Sim2018_COI	S6-13	1	BB87884_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87884	Sample	BB87884	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-13	COI	45	BB87884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51573
BB87884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78507	WGS84	BB87884	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87884_Mgig_Bot2012_COI	S6-13	1	BB87884_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87884	Sample	BB87884	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-13	COI	45	BB87884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.51573
BB87885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78633	WGS84	BB87885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87885_Cmae_Bot2011_COI	S6-12	1	BB87885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87885	Sample	BB87885	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-12	COI	45	BB87885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-12	ATGAACAGTCTATCCTCCTTTAG	138.51551
BB87889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83736	WGS84	BB87889	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87889_Cmae_Bot2011_COI	S6-08	1	BB87889_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87889	Sample	BB87889	targeted	Absent	Sample	Cmae_Bot2011_COI	S6-08	COI	45	BB87889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-08	ATGAACAGTCTATCCTCCTTTAG	138.51038
BB87890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83688	WGS84	BB87890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87890_Aamu_Bax2006_COI	S6-07	1	BB87890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87890	Sample	BB87890	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-07	COI	45	BB87890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-07	GCACAACCGGGATCTTTACTTC	138.50928
BB87890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83688	WGS84	BB87890	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87890_Pcan_Bot2011_IGS	S6-07	1	BB87890_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87890	Sample	BB87890	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-07	IGS	45	BB87890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-07	CGTAATCCTCAGTACTGGCTA	138.50928
BB87890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83688	WGS84	BB87890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87890_Sspa_Oph2007_COI	S6-07	1	BB87890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87890	Sample	BB87890	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-07	COI	45	BB87890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-07	GAAGGCCTTCCGATGATC	138.50928
BB87890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83688	WGS84	BB87890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87890_Upin_Bot2011_COI	S6-07	1	BB87890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87890	Sample	BB87890	targeted	Absent	Sample	Upin_Bot2011_COI	S6-07	COI	45	BB87890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.50928
BB87891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83658	WGS84	BB87891	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87891_Cjap_Sim2018_COI	S6-06	1	BB87891_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87891	Sample	BB87891	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-06	COI	45	BB87891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50805
BB87891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83658	WGS84	BB87891	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87891_Mgig_Bot2012_COI	S6-06	1	BB87891_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87891	Sample	BB87891	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-06	COI	45	BB87891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.50805
BB87894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84522	WGS84	BB87894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87894_Aamu_Bax2006_COI	S6-03	1	BB87894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87894	Sample	BB87894	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-03	COI	45	BB87894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-03	GCACAACCGGGATCTTTACTTC	138.49694
BB87894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84522	WGS84	BB87894	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87894_Pcan_Bot2011_IGS	S6-03	1	BB87894_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87894	Sample	BB87894	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-03	IGS	45	BB87894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-03	CGTAATCCTCAGTACTGGCTA	138.49694
BB87894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84522	WGS84	BB87894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87894_Sspa_Oph2007_COI	S6-03	1	BB87894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87894	Sample	BB87894	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-03	COI	45	BB87894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-03	GAAGGCCTTCCGATGATC	138.49694
BB87894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84522	WGS84	BB87894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87894_Upin_Bot2011_COI	S6-03	1	BB87894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87894	Sample	BB87894	targeted	Absent	Sample	Upin_Bot2011_COI	S6-03	COI	45	BB87894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.49694
BB87895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84619	WGS84	BB87895	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87895_Cjap_Sim2018_COI	S6-02	1	BB87895_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87895	Sample	BB87895	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-02	COI	45	BB87895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49699
BB87895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84619	WGS84	BB87895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87895_Mgig_Bot2012_COI	S6-02	1	BB87895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87895	Sample	BB87895	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-02	COI	45	BB87895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.49699
BB87896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.517	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87896_Cmae_Bot2011_COI	S6-01	1	BB87896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87896	Sample	BB87896	targeted	Present	Sample	Cmae_Bot2011_COI	S6-01	COI	45	BB87896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-01	ATGAACAGTCTATCCTCCTTTAG	138.49739
BB87898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85085	WGS84	BB87898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87898_Aamu_Bax2006_COI	T10-15	1	BB87898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87898	Sample	BB87898	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-15	COI	45	BB87898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-15	GCACAACCGGGATCTTTACTTC	147.87391
BB87898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85085	WGS84	BB87898	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87898_Pcan_Bot2011_IGS	T10-15	1	BB87898_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87898	Sample	BB87898	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-15	IGS	45	BB87898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-15	CGTAATCCTCAGTACTGGCTA	147.87391
BB87898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85085	WGS84	BB87898	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87898_Sspa_Oph2007_COI	T10-15	1	BB87898_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87898	Sample	BB87898	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-15	COI	45	BB87898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-15	GAAGGCCTTCCGATGATC	147.87391
BB87898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85085	WGS84	BB87898	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87898_Upin_Bot2011_COI	T10-15	1	BB87898_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87898	Sample	BB87898	targeted	Absent	Sample	Upin_Bot2011_COI	T10-15	COI	45	BB87898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.87391
BB87899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84692	WGS84	BB87899	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87899_Cjap_Sim2018_COI	T10-14	1	BB87899_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87899	Sample	BB87899	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-14	COI	45	BB87899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87696
BB87899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.84692	WGS84	BB87899	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	29/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87899_Mgig_Bot2012_COI	T10-14	1	BB87899_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87899	Sample	BB87899	targeted	Absent	Sample	Mgig_Bot2012_COI	T10-14	COI	45	BB87899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.87696
BB87900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88961	WGS84	BB87900	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	29/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87900_Cmae_Bot2011_COI	T10-13	1	BB87900_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87900	Sample	BB87900	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-13	COI	45	BB87900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-13	ATGAACAGTCTATCCTCCTTTAG	147.81247
BB87902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88419	WGS84	BB87902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87902_Aamu_Bax2006_COI	T10-11	1	BB87902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87902	Sample	BB87902	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-11	COI	45	BB87902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-11	GCACAACCGGGATCTTTACTTC	147.33632
BB87902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88419	WGS84	BB87902	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87902_Pcan_Bot2011_IGS	T10-11	1	BB87902_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87902	Sample	BB87902	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-11	IGS	45	BB87902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-11	CGTAATCCTCAGTACTGGCTA	147.33632
BB87902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88419	WGS84	BB87902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87902_Sspa_Oph2007_COI	T10-11	1	BB87902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87902	Sample	BB87902	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-11	COI	45	BB87902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-11	GAAGGCCTTCCGATGATC	147.33632
BB87902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88419	WGS84	BB87902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87902_Upin_Bot2011_COI	T10-11	1	BB87902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87902	Sample	BB87902	targeted	Absent	Sample	Upin_Bot2011_COI	T10-11	COI	45	BB87902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33632
BB87903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88552	WGS84	BB87903	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87903_Cjap_Sim2018_COI	T10-10	1	BB87903_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87903	Sample	BB87903	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-10	COI	45	BB87903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33542
BB87903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.88552	WGS84	BB87903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.267	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87903_Mgig_Bot2012_COI	T10-10	1	BB87903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87903	Sample	BB87903	targeted	Present	Sample	Mgig_Bot2012_COI	T10-10	COI	45	BB87903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.33542
BB87904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87881	WGS84	BB87904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87904_Cmae_Bot2011_COI	T10-09	1	BB87904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87904	Sample	BB87904	targeted	Absent	Sample	Cmae_Bot2011_COI	T10-09	COI	45	BB87904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-09	ATGAACAGTCTATCCTCCTTTAG	147.34167
BB87906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8729	WGS84	BB87906	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87906_Aamu_Bax2006_COI	T10-07	1	BB87906_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87906	Sample	BB87906	targeted	Absent	Sample	Aamu_Bax2006_COI	T10-07	COI	45	BB87906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-07	GCACAACCGGGATCTTTACTTC	147.3363
BB87906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8729	WGS84	BB87906	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87906_Pcan_Bot2011_IGS	T10-07	1	BB87906_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87906	Sample	BB87906	targeted	Absent	Sample	Pcan_Bot2011_IGS	T10-07	IGS	45	BB87906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-07	CGTAATCCTCAGTACTGGCTA	147.3363
BB87906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8729	WGS84	BB87906	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87906_Sspa_Oph2007_COI	T10-07	1	BB87906_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87906	Sample	BB87906	targeted	Absent	Sample	Sspa_Oph2007_COI	T10-07	COI	45	BB87906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-07	GAAGGCCTTCCGATGATC	147.3363
BB87906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.8729	WGS84	BB87906	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87906_Upin_Bot2011_COI	T10-07	1	BB87906_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87906	Sample	BB87906	targeted	Absent	Sample	Upin_Bot2011_COI	T10-07	COI	45	BB87906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.3363
BB87907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82895	WGS84	BB87907	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87907_Cjap_Sim2018_COI	T10-06	1	BB87907_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87907	Sample	BB87907	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-06	COI	45	BB87907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30205
BB87907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82895	WGS84	BB87907	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.74	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	28/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87907_Mgig_Bot2012_COI	T10-06	1	BB87907_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87907	Sample	BB87907	targeted	Present	Sample	Mgig_Bot2012_COI	T10-06	COI	45	BB87907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.30205
BB87908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82753	WGS84	BB87908	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.76	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	28/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87908_Cmae_Bot2011_COI	T10-05	1	BB87908_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87908	Sample	BB87908	targeted	Present	Sample	Cmae_Bot2011_COI	T10-05	COI	45	BB87908	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-05	ATGAACAGTCTATCCTCCTTTAG	147.30173
BB87910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96186	WGS84	BB87910	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87910_Cjap_Sim2018_COI	T10-03	1	BB87910_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87910	Sample	BB87910	targeted	Absent	Sample	Cjap_Sim2018_COI	T10-03	COI	45	BB87910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51957
BB87910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96186	WGS84	BB87910	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.722	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T10	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87910_Mgig_Bot2012_COI	T10-03	1	BB87910_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87910	Sample	BB87910	targeted	Present	Sample	Mgig_Bot2012_COI	T10-03	COI	45	BB87910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.51957
BB87911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96019	WGS84	BB87911	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.068	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T10	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87911_Cmae_Bot2011_COI	T10-02	1	BB87911_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87911	Sample	BB87911	targeted	Present	Sample	Cmae_Bot2011_COI	T10-02	COI	45	BB87911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T10-02	ATGAACAGTCTATCCTCCTTTAG	147.53113
BB87915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.81003	WGS84	BB87915	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87915_Cmae_Bot2011_COI	S5-22	1	BB87915_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87915	Sample	BB87915	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-22	COI	45	BB87915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-22	ATGAACAGTCTATCCTCCTTTAG	138.52013
BB87919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77349	WGS84	BB87919	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87919_Cmae_Bot2011_COI	S5-18	1	BB87919_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87919	Sample	BB87919	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-18	COI	45	BB87919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-18	ATGAACAGTCTATCCTCCTTTAG	138.48577
BB87920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77247	WGS84	BB87920	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87920_Aamu_Bax2006_COI	S5-17	1	BB87920_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87920	Sample	BB87920	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-17	COI	45	BB87920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-17	GCACAACCGGGATCTTTACTTC	138.4855
BB87920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77247	WGS84	BB87920	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87920_Pcan_Bot2011_IGS	S5-17	1	BB87920_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87920	Sample	BB87920	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-17	IGS	45	BB87920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-17	CGTAATCCTCAGTACTGGCTA	138.4855
BB87920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77247	WGS84	BB87920	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.972	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87920_Sspa_Oph2007_COI	S5-17	1	BB87920_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87920	Sample	BB87920	targeted	Present	Sample	Sspa_Oph2007_COI	S5-17	COI	45	BB87920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-17	GAAGGCCTTCCGATGATC	138.4855
BB87920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77247	WGS84	BB87920	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87920_Upin_Bot2011_COI	S5-17	1	BB87920_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87920	Sample	BB87920	targeted	Absent	Sample	Upin_Bot2011_COI	S5-17	COI	45	BB87920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-17	CTTTAATTACAGCGTTTTTATTGTTGT	138.4855
BB87921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.77137	WGS84	BB87921	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87921_Cjap_Sim2018_COI	S5-16	1	BB87921_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87921	Sample	BB87921	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-16	COI	45	BB87921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48612
BB87921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.77137	WGS84	BB87921	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87921_Mgig_Bot2012_COI	S5-16	1	BB87921_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87921	Sample	BB87921	targeted	Absent	Sample	Mgig_Bot2012_COI	S5-16	COI	45	BB87921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-16	TCTTATTCGTTGGAGACTTTATAACCCT	138.48612
BB87924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78534	WGS84	BB87924	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87924_Aamu_Bax2006_COI	S5-13	1	BB87924_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87924	Sample	BB87924	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-13	COI	45	BB87924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-13	GCACAACCGGGATCTTTACTTC	138.51572
BB87924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78534	WGS84	BB87924	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87924_Pcan_Bot2011_IGS	S5-13	1	BB87924_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87924	Sample	BB87924	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-13	IGS	45	BB87924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-13	CGTAATCCTCAGTACTGGCTA	138.51572
BB87924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78534	WGS84	BB87924	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.382	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87924_Sspa_Oph2007_COI	S5-13	1	BB87924_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87924	Sample	BB87924	targeted	Present	Sample	Sspa_Oph2007_COI	S5-13	COI	45	BB87924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-13	GAAGGCCTTCCGATGATC	138.51572
BB87924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78534	WGS84	BB87924	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87924_Upin_Bot2011_COI	S5-13	1	BB87924_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87924	Sample	BB87924	targeted	Absent	Sample	Upin_Bot2011_COI	S5-13	COI	45	BB87924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-13	CTTTAATTACAGCGTTTTTATTGTTGT	138.51572
BB87925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78656	WGS84	BB87925	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87925_Cjap_Sim2018_COI	S5-12	1	BB87925_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87925	Sample	BB87925	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-12	COI	45	BB87925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51548
BB87925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.78656	WGS84	BB87925	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.252	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87925_Mgig_Bot2012_COI	S5-12	1	BB87925_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87925	Sample	BB87925	targeted	Present	Sample	Mgig_Bot2012_COI	S5-12	COI	45	BB87925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.51548
BB87926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.78764	WGS84	BB87926	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87926_Cmae_Bot2011_COI	S5-11	1	BB87926_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87926	Sample	BB87926	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-11	COI	45	BB87926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-11	ATGAACAGTCTATCCTCCTTTAG	138.51513
BB87929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83656	WGS84	BB87929	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87929_Cjap_Sim2018_COI	S5-08	1	BB87929_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87929	Sample	BB87929	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-08	COI	45	BB87929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50972
BB87929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.83656	WGS84	BB87929	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.685	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87929_Mgig_Bot2012_COI	S5-08	1	BB87929_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87929	Sample	BB87929	targeted	Present	Sample	Mgig_Bot2012_COI	S5-08	COI	45	BB87929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.50972
BB87931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87931	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87931_Aamu_Bax2006_COI	S5-06	1	BB87931_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87931	Sample	BB87931	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-06	COI	45	BB87931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-06	GCACAACCGGGATCTTTACTTC	138.50759
BB87931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87931	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87931_Pcan_Bot2011_IGS	S5-06	1	BB87931_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87931	Sample	BB87931	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-06	IGS	45	BB87931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-06	CGTAATCCTCAGTACTGGCTA	138.50759
BB87931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	27.483	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87931_Sspa_Oph2007_COI	S5-06	1	BB87931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87931	Sample	BB87931	targeted	Present	Sample	Sspa_Oph2007_COI	S5-06	COI	45	BB87931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-06	GAAGGCCTTCCGATGATC	138.50759
BB87931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.83703	WGS84	BB87931	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87931_Upin_Bot2011_COI	S5-06	1	BB87931_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87931	Sample	BB87931	targeted	Absent	Sample	Upin_Bot2011_COI	S5-06	COI	45	BB87931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.50759
BB87935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84612	WGS84	BB87935	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87935_Aamu_Bax2006_COI	S5-02	1	BB87935_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87935	Sample	BB87935	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-02	COI	45	BB87935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-02	GCACAACCGGGATCTTTACTTC	138.49706
BB87935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84612	WGS84	BB87935	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87935_Pcan_Bot2011_IGS	S5-02	1	BB87935_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87935	Sample	BB87935	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-02	IGS	45	BB87935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-02	CGTAATCCTCAGTACTGGCTA	138.49706
BB87935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84612	WGS84	BB87935	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87935_Sspa_Oph2007_COI	S5-02	1	BB87935_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87935	Sample	BB87935	targeted	Absent	Sample	Sspa_Oph2007_COI	S5-02	COI	45	BB87935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-02	GAAGGCCTTCCGATGATC	138.49706
BB87935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84612	WGS84	BB87935	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87935_Upin_Bot2011_COI	S5-02	1	BB87935_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87935	Sample	BB87935	targeted	Absent	Sample	Upin_Bot2011_COI	S5-02	COI	45	BB87935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.49706
BB87936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.84698	WGS84	BB87936	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87936_Cjap_Sim2018_COI	S5-01	1	BB87936_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87936	Sample	BB87936	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-01	COI	45	BB87936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49736
BB87936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10632	-34.84698	WGS84	BB87936	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.676	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87936_Mgig_Bot2012_COI	S5-01	1	BB87936_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87936	Sample	BB87936	targeted	Present	Sample	Mgig_Bot2012_COI	S5-01	COI	45	BB87936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.49736
BB87937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88278	WGS84	BB87937	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87937_Cmae_Bot2011_COI	T9-16	1	BB87937_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87937	Sample	BB87937	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-16	COI	45	BB87937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-16	ATGAACAGTCTATCCTCCTTTAG	147.33629
BB87939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88567	WGS84	BB87939	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87939_Aamu_Bax2006_COI	T9-14	1	BB87939_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87939	Sample	BB87939	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-14	COI	45	BB87939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-14	GCACAACCGGGATCTTTACTTC	147.33529
BB87939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88567	WGS84	BB87939	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87939_Pcan_Bot2011_IGS	T9-14	1	BB87939_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87939	Sample	BB87939	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-14	IGS	45	BB87939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-14	CGTAATCCTCAGTACTGGCTA	147.33529
BB87939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88567	WGS84	BB87939	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87939_Sspa_Oph2007_COI	T9-14	1	BB87939_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87939	Sample	BB87939	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-14	COI	45	BB87939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-14	GAAGGCCTTCCGATGATC	147.33529
BB87939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.88567	WGS84	BB87939	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87939_Upin_Bot2011_COI	T9-14	1	BB87939_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87939	Sample	BB87939	targeted	Absent	Sample	Upin_Bot2011_COI	T9-14	COI	45	BB87939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.33529
BB87940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87919	WGS84	BB87940	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87940_Cmae_Bot2011_COI	T9-13	1	BB87940_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87940	Sample	BB87940	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-13	COI	45	BB87940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-13	ATGAACAGTCTATCCTCCTTTAG	147.3417
BB87942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87302	WGS84	BB87942	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.11	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87942_Aamu_Bax2006_COI	T9-11	1	BB87942_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87942	Sample	BB87942	targeted	Present	Sample	Aamu_Bax2006_COI	T9-11	COI	45	BB87942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-11	GCACAACCGGGATCTTTACTTC	147.33632
BB87942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87302	WGS84	BB87942	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87942_Pcan_Bot2011_IGS	T9-11	1	BB87942_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87942	Sample	BB87942	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-11	IGS	45	BB87942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-11	CGTAATCCTCAGTACTGGCTA	147.33632
BB87942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87302	WGS84	BB87942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87942_Sspa_Oph2007_COI	T9-11	1	BB87942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87942	Sample	BB87942	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-11	COI	45	BB87942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-11	GAAGGCCTTCCGATGATC	147.33632
BB87942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.87302	WGS84	BB87942	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87942_Upin_Bot2011_COI	T9-11	1	BB87942_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87942	Sample	BB87942	targeted	Absent	Sample	Upin_Bot2011_COI	T9-11	COI	45	BB87942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33632
BB87943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82517	WGS84	BB87943	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87943_Cjap_Sim2018_COI	T9-10	1	BB87943_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87943	Sample	BB87943	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-10	COI	45	BB87943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30705
BB87943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.82517	WGS84	BB87943	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.865	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87943_Mgig_Bot2012_COI	T9-10	1	BB87943_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87943	Sample	BB87943	targeted	Present	Sample	Mgig_Bot2012_COI	T9-10	COI	45	BB87943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.30705
BB87944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.82698	WGS84	BB87944	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87944_Cmae_Bot2011_COI	T9-09	1	BB87944_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87944	Sample	BB87944	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-09	COI	45	BB87944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-09	ATGAACAGTCTATCCTCCTTTAG	147.30243
BB87946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85585	WGS84	BB87946	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87946_Aamu_Bax2006_COI	T9-07	1	BB87946_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87946	Sample	BB87946	targeted	Absent	Sample	Aamu_Bax2006_COI	T9-07	COI	45	BB87946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-07	GCACAACCGGGATCTTTACTTC	147.87239
BB87946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85585	WGS84	BB87946	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87946_Pcan_Bot2011_IGS	T9-07	1	BB87946_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87946	Sample	BB87946	targeted	Absent	Sample	Pcan_Bot2011_IGS	T9-07	IGS	45	BB87946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-07	CGTAATCCTCAGTACTGGCTA	147.87239
BB87946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85585	WGS84	BB87946	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87946_Sspa_Oph2007_COI	T9-07	1	BB87946_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87946	Sample	BB87946	targeted	Absent	Sample	Sspa_Oph2007_COI	T9-07	COI	45	BB87946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-07	GAAGGCCTTCCGATGATC	147.87239
BB87946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85585	WGS84	BB87946	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87946_Upin_Bot2011_COI	T9-07	1	BB87946_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87946	Sample	BB87946	targeted	Absent	Sample	Upin_Bot2011_COI	T9-07	COI	45	BB87946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.87239
BB87947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.85078	WGS84	BB87947	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87947_Cjap_Sim2018_COI	T9-06	1	BB87947_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87947	Sample	BB87947	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-06	COI	45	BB87947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87365
BB87947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.85078	WGS84	BB87947	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.406	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	23/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87947_Mgig_Bot2012_COI	T9-06	1	BB87947_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87947	Sample	BB87947	targeted	Present	Sample	Mgig_Bot2012_COI	T9-06	COI	45	BB87947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.87365
BB87948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.84699	WGS84	BB87948	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	23/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87948_Cmae_Bot2011_COI	T9-05	1	BB87948_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87948	Sample	BB87948	targeted	Absent	Sample	Cmae_Bot2011_COI	T9-05	COI	45	BB87948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-05	ATGAACAGTCTATCCTCCTTTAG	147.87517
BB87950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96213	WGS84	BB87950	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB87950_Cjap_Sim2018_COI	T9-03	1	BB87950_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87950	Sample	BB87950	targeted	Absent	Sample	Cjap_Sim2018_COI	T9-03	COI	45	BB87950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51947
BB87950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10630	-42.96213	WGS84	BB87950	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.075	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T09	22/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87950_Mgig_Bot2012_COI	T9-03	1	BB87950_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87950	Sample	BB87950	targeted	Present	Sample	Mgig_Bot2012_COI	T9-03	COI	45	BB87950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.51947
BB87951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13000	-42.96033	WGS84	BB87951	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.593	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T09	22/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87951_Cmae_Bot2011_COI	T9-02	1	BB87951_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87951	Sample	BB87951	targeted	Present	Sample	Cmae_Bot2011_COI	T9-02	COI	45	BB87951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T9-02	ATGAACAGTCTATCCTCCTTTAG	147.53072
BB87954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.81014	WGS84	BB87954	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.64	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87954_Mgig_Bot2012_COI	S4-19	1	BB87954_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87954	Sample	BB87954	targeted	Present	Sample	Mgig_Bot2012_COI	S4-19	COI	45	BB87954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.52049
BB87955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80982	WGS84	BB87955	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB87955_Cmae_Bot2011_COI	S4-18	1	BB87955_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87955	Sample	BB87955	targeted	Absent	Sample	Cmae_Bot2011_COI	S4-18	COI	45	BB87955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-18	ATGAACAGTCTATCCTCCTTTAG	138.51977
BB87957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80913	WGS84	BB87957	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB87957_Aamu_Bax2006_COI	S4-16	1	BB87957_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87957	Sample	BB87957	targeted	Absent	Sample	Aamu_Bax2006_COI	S4-16	COI	45	BB87957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-16	GCACAACCGGGATCTTTACTTC	138.51747
BB87957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80913	WGS84	BB87957	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB87957_Pcan_Bot2011_IGS	S4-16	1	BB87957_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87957	Sample	BB87957	targeted	Absent	Sample	Pcan_Bot2011_IGS	S4-16	IGS	45	BB87957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-16	CGTAATCCTCAGTACTGGCTA	138.51747
BB87957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80913	WGS84	BB87957	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.982	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB87957_Sspa_Oph2007_COI	S4-16	1	BB87957_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87957	Sample	BB87957	targeted	Present	Sample	Sspa_Oph2007_COI	S4-16	COI	45	BB87957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-16	GAAGGCCTTCCGATGATC	138.51747
BB87957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13001	-34.80913	WGS84	BB87957	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S04	21/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB87957_Upin_Bot2011_COI	S4-16	1	BB87957_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87957	Sample	BB87957	targeted	Absent	Sample	Upin_Bot2011_COI	S4-16	COI	45	BB87957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-16	CTTTAATTACAGCGTTTTTATTGTTGT	138.51747
BB87958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83687	WGS84	BB87958	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.019	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S04	21/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB87958_Mgig_Bot2012_COI	S4-14	1	BB87958_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB87958	Sample	BB87958	targeted	Present	Sample	Mgig_Bot2012_COI	S4-14	COI	45	BB87958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S4-14	TCTTATTCGTTGGAGACTTTATAACCCT	138.5096
BB93410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18844	WGS84	BB93410	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93410_Mgig_Bot2012_COI	DD4	1	BB93410_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93410	Sample	BB93410	targeted	Absent	Sample	Mgig_Bot2012_COI	DD4	COI	45	BB93410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD4	TCTTATTCGTTGGAGACTTTATAACCCT	146.36786
BB93413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85318	WGS84	BB93413	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93413_Aamu_Bax2006_COI	GD90	1	BB93413_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93413	Sample	BB93413	targeted	Absent	Sample	Aamu_Bax2006_COI	GD90	COI	45	BB93413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD90	GCACAACCGGGATCTTTACTTC	151.31829
BB93413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85318	WGS84	BB93413	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93413_Pcan_Bot2011_IGS	GD90	1	BB93413_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93413	Sample	BB93413	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD90	IGS	45	BB93413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD90	CGTAATCCTCAGTACTGGCTA	151.31829
BB93413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85318	WGS84	BB93413	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93413_Sspa_Oph2007_COI	GD90	1	BB93413_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93413	Sample	BB93413	targeted	Absent	Sample	Sspa_Oph2007_COI	GD90	COI	45	BB93413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD90	GAAGGCCTTCCGATGATC	151.31829
BB93413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85318	WGS84	BB93413	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93413_Upin_Bot2011_COI	GD90	1	BB93413_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93413	Sample	BB93413	targeted	Absent	Sample	Upin_Bot2011_COI	GD90	COI	45	BB93413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD90	CTTTAATTACAGCGTTTTTATTGTTGT	151.31829
BB93414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7897	WGS84	BB93414	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93414_Mgig_Bot2012_COI	GD80	1	BB93414_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93414	Sample	BB93414	targeted	Absent	Sample	Mgig_Bot2012_COI	GD80	COI	45	BB93414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD80	TCTTATTCGTTGGAGACTTTATAACCCT	151.20943
BB93415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78606	WGS84	BB93415	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93415_Cmae_Bot2011_COI	GD70	1	BB93415_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93415	Sample	BB93415	targeted	Absent	Sample	Cmae_Bot2011_COI	GD70	COI	45	BB93415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD70	ATGAACAGTCTATCCTCCTTTAG	151.17164
BB93415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78606	WGS84	BB93415	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93415_Msal_Bot2012_COI	GD70	1	BB93415_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93415	Sample	BB93415	targeted	Absent	Sample	Msal_Bot2012_COI	GD70	COI	45	BB93415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD70	GYTAGTTCCRATGATGTTAGCTG	151.17164
BB93417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79059	WGS84	BB93417	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93417_Aamu_Bax2006_COI	GD60	1	BB93417_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93417	Sample	BB93417	targeted	Absent	Sample	Aamu_Bax2006_COI	GD60	COI	45	BB93417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD60	GCACAACCGGGATCTTTACTTC	151.17623
BB93417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79059	WGS84	BB93417	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93417_Pcan_Bot2011_IGS	GD60	1	BB93417_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93417	Sample	BB93417	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD60	IGS	45	BB93417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD60	CGTAATCCTCAGTACTGGCTA	151.17623
BB93417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79059	WGS84	BB93417	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93417_Sspa_Oph2007_COI	GD60	1	BB93417_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93417	Sample	BB93417	targeted	Absent	Sample	Sspa_Oph2007_COI	GD60	COI	45	BB93417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD60	GAAGGCCTTCCGATGATC	151.17623
BB93417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79059	WGS84	BB93417	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93417_Upin_Bot2011_COI	GD60	1	BB93417_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93417	Sample	BB93417	targeted	Absent	Sample	Upin_Bot2011_COI	GD60	COI	45	BB93417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD60	CTTTAATTACAGCGTTTTTATTGTTGT	151.17623
BB93418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80527	WGS84	BB93418	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93418_Mgig_Bot2012_COI	GD50	1	BB93418_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93418	Sample	BB93418	targeted	Absent	Sample	Mgig_Bot2012_COI	GD50	COI	45	BB93418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD50	TCTTATTCGTTGGAGACTTTATAACCCT	151.21539
BB93419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81656	WGS84	BB93419	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93419_Cmae_Bot2011_COI	GD107	1	BB93419_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93419	Sample	BB93419	targeted	Absent	Sample	Cmae_Bot2011_COI	GD107	COI	45	BB93419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD107	ATGAACAGTCTATCCTCCTTTAG	151.24038
BB93419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81656	WGS84	BB93419	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93419_Msal_Bot2012_COI	GD107	1	BB93419_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93419	Sample	BB93419	targeted	Absent	Sample	Msal_Bot2012_COI	GD107	COI	45	BB93419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD107	GYTAGTTCCRATGATGTTAGCTG	151.24038
BB93420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82257	WGS84	BB93420	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93420_Aamu_Bax2006_COI	GD111	1	BB93420_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93420	Sample	BB93420	targeted	Absent	Sample	Aamu_Bax2006_COI	GD111	COI	45	BB93420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD111	GCACAACCGGGATCTTTACTTC	151.24626
BB93420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82257	WGS84	BB93420	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93420_Pcan_Bot2011_IGS	GD111	1	BB93420_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93420	Sample	BB93420	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD111	IGS	45	BB93420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD111	CGTAATCCTCAGTACTGGCTA	151.24626
BB93420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82257	WGS84	BB93420	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93420_Sspa_Oph2007_COI	GD111	1	BB93420_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93420	Sample	BB93420	targeted	Absent	Sample	Sspa_Oph2007_COI	GD111	COI	45	BB93420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD111	GAAGGCCTTCCGATGATC	151.24626
BB93420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82257	WGS84	BB93420	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93420_Upin_Bot2011_COI	GD111	1	BB93420_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93420	Sample	BB93420	targeted	Absent	Sample	Upin_Bot2011_COI	GD111	COI	45	BB93420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD111	CTTTAATTACAGCGTTTTTATTGTTGT	151.24626
BB93421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83117	WGS84	BB93421	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93421_Mgig_Bot2012_COI	GD34	1	BB93421_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93421	Sample	BB93421	targeted	Absent	Sample	Mgig_Bot2012_COI	GD34	COI	45	BB93421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD34	TCTTATTCGTTGGAGACTTTATAACCCT	151.25505
BB93422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83171	WGS84	BB93422	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93422_Cmae_Bot2011_COI	GD36	1	BB93422_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93422	Sample	BB93422	targeted	Absent	Sample	Cmae_Bot2011_COI	GD36	COI	45	BB93422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD36	ATGAACAGTCTATCCTCCTTTAG	151.25897
BB93422	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83171	WGS84	BB93422	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93422_Msal_Bot2012_COI	GD36	1	BB93422_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93422	Sample	BB93422	targeted	Absent	Sample	Msal_Bot2012_COI	GD36	COI	45	BB93422	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD36	GYTAGTTCCRATGATGTTAGCTG	151.25897
BB93424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8344	WGS84	BB93424	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93424_Aamu_Bax2006_COI	GD39	1	BB93424_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93424	Sample	BB93424	targeted	Absent	Sample	Aamu_Bax2006_COI	GD39	COI	45	BB93424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD39	GCACAACCGGGATCTTTACTTC	151.26551
BB93424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8344	WGS84	BB93424	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93424_Pcan_Bot2011_IGS	GD39	1	BB93424_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93424	Sample	BB93424	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD39	IGS	45	BB93424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD39	CGTAATCCTCAGTACTGGCTA	151.26551
BB93424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8344	WGS84	BB93424	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93424_Sspa_Oph2007_COI	GD39	1	BB93424_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93424	Sample	BB93424	targeted	Absent	Sample	Sspa_Oph2007_COI	GD39	COI	45	BB93424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD39	GAAGGCCTTCCGATGATC	151.26551
BB93424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8344	WGS84	BB93424	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93424_Upin_Bot2011_COI	GD39	1	BB93424_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93424	Sample	BB93424	targeted	Absent	Sample	Upin_Bot2011_COI	GD39	COI	45	BB93424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD39	CTTTAATTACAGCGTTTTTATTGTTGT	151.26551
BB93425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83282	WGS84	BB93425	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93425_Mgig_Bot2012_COI	GD42	1	BB93425_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93425	Sample	BB93425	targeted	Absent	Sample	Mgig_Bot2012_COI	GD42	COI	45	BB93425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD42	TCTTATTCGTTGGAGACTTTATAACCCT	151.26352
BB93426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8062	WGS84	BB93426	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93426_Cmae_Bot2011_COI	GD43	1	BB93426_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93426	Sample	BB93426	targeted	Absent	Sample	Cmae_Bot2011_COI	GD43	COI	45	BB93426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD43	ATGAACAGTCTATCCTCCTTTAG	151.22027
BB93426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8062	WGS84	BB93426	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93426_Msal_Bot2012_COI	GD43	1	BB93426_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93426	Sample	BB93426	targeted	Absent	Sample	Msal_Bot2012_COI	GD43	COI	45	BB93426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD43	GYTAGTTCCRATGATGTTAGCTG	151.22027
BB93428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83454	WGS84	BB93428	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93428_Aamu_Bax2006_COI	GD103	1	BB93428_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93428	Sample	BB93428	targeted	Absent	Sample	Aamu_Bax2006_COI	GD103	COI	45	BB93428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD103	GCACAACCGGGATCTTTACTTC	151.26959
BB93428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83454	WGS84	BB93428	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93428_Pcan_Bot2011_IGS	GD103	1	BB93428_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93428	Sample	BB93428	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD103	IGS	45	BB93428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD103	CGTAATCCTCAGTACTGGCTA	151.26959
BB93428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83454	WGS84	BB93428	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93428_Sspa_Oph2007_COI	GD103	1	BB93428_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93428	Sample	BB93428	targeted	Absent	Sample	Sspa_Oph2007_COI	GD103	COI	45	BB93428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD103	GAAGGCCTTCCGATGATC	151.26959
BB93428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83454	WGS84	BB93428	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93428_Upin_Bot2011_COI	GD103	1	BB93428_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93428	Sample	BB93428	targeted	Absent	Sample	Upin_Bot2011_COI	GD103	COI	45	BB93428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD103	CTTTAATTACAGCGTTTTTATTGTTGT	151.26959
BB93429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80704	WGS84	BB93429	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93429_Mgig_Bot2012_COI	GD47	1	BB93429_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93429	Sample	BB93429	targeted	Absent	Sample	Mgig_Bot2012_COI	GD47	COI	45	BB93429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD47	TCTTATTCGTTGGAGACTTTATAACCCT	151.21656
BB93431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80543	WGS84	BB93431	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93431_Aamu_Bax2006_COI	GD52	1	BB93431_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93431	Sample	BB93431	targeted	Absent	Sample	Aamu_Bax2006_COI	GD52	COI	45	BB93431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD52	GCACAACCGGGATCTTTACTTC	151.21816
BB93431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80543	WGS84	BB93431	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93431_Pcan_Bot2011_IGS	GD52	1	BB93431_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93431	Sample	BB93431	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD52	IGS	45	BB93431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD52	CGTAATCCTCAGTACTGGCTA	151.21816
BB93431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80543	WGS84	BB93431	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93431_Sspa_Oph2007_COI	GD52	1	BB93431_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93431	Sample	BB93431	targeted	Absent	Sample	Sspa_Oph2007_COI	GD52	COI	45	BB93431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD52	GAAGGCCTTCCGATGATC	151.21816
BB93431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80543	WGS84	BB93431	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93431_Upin_Bot2011_COI	GD52	1	BB93431_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93431	Sample	BB93431	targeted	Absent	Sample	Upin_Bot2011_COI	GD52	COI	45	BB93431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD52	CTTTAATTACAGCGTTTTTATTGTTGT	151.21816
BB93432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79141	WGS84	BB93432	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93432_Mgig_Bot2012_COI	GD54	1	BB93432_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93432	Sample	BB93432	targeted	Absent	Sample	Mgig_Bot2012_COI	GD54	COI	45	BB93432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD54	TCTTATTCGTTGGAGACTTTATAACCCT	151.17851
BB93433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79121	WGS84	BB93433	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93433_Cmae_Bot2011_COI	GD56	1	BB93433_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93433	Sample	BB93433	targeted	Absent	Sample	Cmae_Bot2011_COI	GD56	COI	45	BB93433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD56	ATGAACAGTCTATCCTCCTTTAG	151.18219
BB93433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79121	WGS84	BB93433	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93433_Msal_Bot2012_COI	GD56	1	BB93433_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93433	Sample	BB93433	targeted	Absent	Sample	Msal_Bot2012_COI	GD56	COI	45	BB93433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD56	GYTAGTTCCRATGATGTTAGCTG	151.18219
BB93435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78916	WGS84	BB93435	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93435_Aamu_Bax2006_COI	GD61	1	BB93435_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93435	Sample	BB93435	targeted	Absent	Sample	Aamu_Bax2006_COI	GD61	COI	45	BB93435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD61	GCACAACCGGGATCTTTACTTC	151.17678
BB93435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78916	WGS84	BB93435	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93435_Pcan_Bot2011_IGS	GD61	1	BB93435_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93435	Sample	BB93435	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD61	IGS	45	BB93435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD61	CGTAATCCTCAGTACTGGCTA	151.17678
BB93435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78916	WGS84	BB93435	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93435_Sspa_Oph2007_COI	GD61	1	BB93435_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93435	Sample	BB93435	targeted	Absent	Sample	Sspa_Oph2007_COI	GD61	COI	45	BB93435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD61	GAAGGCCTTCCGATGATC	151.17678
BB93435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78916	WGS84	BB93435	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93435_Upin_Bot2011_COI	GD61	1	BB93435_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93435	Sample	BB93435	targeted	Absent	Sample	Upin_Bot2011_COI	GD61	COI	45	BB93435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD61	CTTTAATTACAGCGTTTTTATTGTTGT	151.17678
BB93436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78713	WGS84	BB93436	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93436_Mgig_Bot2012_COI	GD63	1	BB93436_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93436	Sample	BB93436	targeted	Absent	Sample	Mgig_Bot2012_COI	GD63	COI	45	BB93436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD63	TCTTATTCGTTGGAGACTTTATAACCCT	151.17623
BB93437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78794	WGS84	BB93437	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93437_Cmae_Bot2011_COI	GD66	1	BB93437_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93437	Sample	BB93437	targeted	Absent	Sample	Cmae_Bot2011_COI	GD66	COI	45	BB93437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD66	ATGAACAGTCTATCCTCCTTTAG	151.17475
BB93437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78794	WGS84	BB93437	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93437_Msal_Bot2012_COI	GD66	1	BB93437_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93437	Sample	BB93437	targeted	Absent	Sample	Msal_Bot2012_COI	GD66	COI	45	BB93437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD66	GYTAGTTCCRATGATGTTAGCTG	151.17475
BB93439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78618	WGS84	BB93439	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93439_Aamu_Bax2006_COI	GD69	1	BB93439_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93439	Sample	BB93439	targeted	Absent	Sample	Aamu_Bax2006_COI	GD69	COI	45	BB93439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD69	GCACAACCGGGATCTTTACTTC	151.17238
BB93439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78618	WGS84	BB93439	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93439_Pcan_Bot2011_IGS	GD69	1	BB93439_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93439	Sample	BB93439	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD69	IGS	45	BB93439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD69	CGTAATCCTCAGTACTGGCTA	151.17238
BB93439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78618	WGS84	BB93439	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93439_Sspa_Oph2007_COI	GD69	1	BB93439_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93439	Sample	BB93439	targeted	Absent	Sample	Sspa_Oph2007_COI	GD69	COI	45	BB93439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD69	GAAGGCCTTCCGATGATC	151.17238
BB93439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78618	WGS84	BB93439	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93439_Upin_Bot2011_COI	GD69	1	BB93439_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93439	Sample	BB93439	targeted	Absent	Sample	Upin_Bot2011_COI	GD69	COI	45	BB93439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD69	CTTTAATTACAGCGTTTTTATTGTTGT	151.17238
BB93440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7776	WGS84	BB93440	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93440_Cmae_Bot2011_COI	GD72	1	BB93440_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93440	Sample	BB93440	targeted	Absent	Sample	Cmae_Bot2011_COI	GD72	COI	45	BB93440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD72	ATGAACAGTCTATCCTCCTTTAG	151.19514
BB93440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.7776	WGS84	BB93440	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93440_Msal_Bot2012_COI	GD72	1	BB93440_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93440	Sample	BB93440	targeted	Absent	Sample	Msal_Bot2012_COI	GD72	COI	45	BB93440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD72	GYTAGTTCCRATGATGTTAGCTG	151.19514
BB93442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78083	WGS84	BB93442	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93442_Aamu_Bax2006_COI	GD76	1	BB93442_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93442	Sample	BB93442	targeted	Absent	Sample	Aamu_Bax2006_COI	GD76	COI	45	BB93442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD76	GCACAACCGGGATCTTTACTTC	151.1992
BB93442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78083	WGS84	BB93442	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93442_Pcan_Bot2011_IGS	GD76	1	BB93442_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93442	Sample	BB93442	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD76	IGS	45	BB93442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD76	CGTAATCCTCAGTACTGGCTA	151.1992
BB93442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78083	WGS84	BB93442	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93442_Sspa_Oph2007_COI	GD76	1	BB93442_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93442	Sample	BB93442	targeted	Absent	Sample	Sspa_Oph2007_COI	GD76	COI	45	BB93442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD76	GAAGGCCTTCCGATGATC	151.1992
BB93442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78083	WGS84	BB93442	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93442_Upin_Bot2011_COI	GD76	1	BB93442_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93442	Sample	BB93442	targeted	Absent	Sample	Upin_Bot2011_COI	GD76	COI	45	BB93442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD76	CTTTAATTACAGCGTTTTTATTGTTGT	151.1992
BB93443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78389	WGS84	BB93443	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93443_Mgig_Bot2012_COI	GD78	1	BB93443_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93443	Sample	BB93443	targeted	Absent	Sample	Mgig_Bot2012_COI	GD78	COI	45	BB93443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD78	TCTTATTCGTTGGAGACTTTATAACCCT	151.20134
BB93444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78485	WGS84	BB93444	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93444_Cmae_Bot2011_COI	GD79	1	BB93444_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93444	Sample	BB93444	targeted	Absent	Sample	Cmae_Bot2011_COI	GD79	COI	45	BB93444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD79	ATGAACAGTCTATCCTCCTTTAG	151.20342
BB93444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.78485	WGS84	BB93444	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93444_Msal_Bot2012_COI	GD79	1	BB93444_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93444	Sample	BB93444	targeted	Absent	Sample	Msal_Bot2012_COI	GD79	COI	45	BB93444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD79	GYTAGTTCCRATGATGTTAGCTG	151.20342
BB93446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79468	WGS84	BB93446	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93446_Aamu_Bax2006_COI	GD84	1	BB93446_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93446	Sample	BB93446	targeted	Absent	Sample	Aamu_Bax2006_COI	GD84	COI	45	BB93446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD84	GCACAACCGGGATCTTTACTTC	151.21374
BB93446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79468	WGS84	BB93446	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93446_Pcan_Bot2011_IGS	GD84	1	BB93446_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93446	Sample	BB93446	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD84	IGS	45	BB93446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD84	CGTAATCCTCAGTACTGGCTA	151.21374
BB93446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79468	WGS84	BB93446	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93446_Sspa_Oph2007_COI	GD84	1	BB93446_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93446	Sample	BB93446	targeted	Absent	Sample	Sspa_Oph2007_COI	GD84	COI	45	BB93446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD84	GAAGGCCTTCCGATGATC	151.21374
BB93446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.79468	WGS84	BB93446	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93446_Upin_Bot2011_COI	GD84	1	BB93446_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93446	Sample	BB93446	targeted	Absent	Sample	Upin_Bot2011_COI	GD84	COI	45	BB93446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD84	CTTTAATTACAGCGTTTTTATTGTTGT	151.21374
BB93447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85551	WGS84	BB93447	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93447_Mgig_Bot2012_COI	GD86	1	BB93447_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93447	Sample	BB93447	targeted	Absent	Sample	Mgig_Bot2012_COI	GD86	COI	45	BB93447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD86	TCTTATTCGTTGGAGACTTTATAACCCT	151.32168
BB93448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85314	WGS84	BB93448	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93448_Cmae_Bot2011_COI	GD88	1	BB93448_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93448	Sample	BB93448	targeted	Absent	Sample	Cmae_Bot2011_COI	GD88	COI	45	BB93448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD88	ATGAACAGTCTATCCTCCTTTAG	151.31966
BB93448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85314	WGS84	BB93448	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93448_Msal_Bot2012_COI	GD88	1	BB93448_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93448	Sample	BB93448	targeted	Absent	Sample	Msal_Bot2012_COI	GD88	COI	45	BB93448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD88	GYTAGTTCCRATGATGTTAGCTG	151.31966
BB93450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85126	WGS84	BB93450	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93450_Mgig_Bot2012_COI	GD93	1	BB93450_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93450	Sample	BB93450	targeted	Absent	Sample	Mgig_Bot2012_COI	GD93	COI	45	BB93450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD93	TCTTATTCGTTGGAGACTTTATAACCCT	151.31393
BB93451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85305	WGS84	BB93451	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93451_Cmae_Bot2011_COI	GD97	1	BB93451_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93451	Sample	BB93451	targeted	Absent	Sample	Cmae_Bot2011_COI	GD97	COI	45	BB93451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD97	ATGAACAGTCTATCCTCCTTTAG	151.3086
BB93451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.85305	WGS84	BB93451	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93451_Msal_Bot2012_COI	GD97	1	BB93451_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93451	Sample	BB93451	targeted	Absent	Sample	Msal_Bot2012_COI	GD97	COI	45	BB93451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD97	GYTAGTTCCRATGATGTTAGCTG	151.3086
BB93453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82128	WGS84	BB93453	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93453_Aamu_Bax2006_COI	GD15	1	BB93453_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93453	Sample	BB93453	targeted	Absent	Sample	Aamu_Bax2006_COI	GD15	COI	45	BB93453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD15	GCACAACCGGGATCTTTACTTC	151.24058
BB93453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82128	WGS84	BB93453	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93453_Pcan_Bot2011_IGS	GD15	1	BB93453_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93453	Sample	BB93453	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD15	IGS	45	BB93453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD15	CGTAATCCTCAGTACTGGCTA	151.24058
BB93453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82128	WGS84	BB93453	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93453_Sspa_Oph2007_COI	GD15	1	BB93453_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93453	Sample	BB93453	targeted	Absent	Sample	Sspa_Oph2007_COI	GD15	COI	45	BB93453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD15	GAAGGCCTTCCGATGATC	151.24058
BB93453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82128	WGS84	BB93453	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93453_Upin_Bot2011_COI	GD15	1	BB93453_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93453	Sample	BB93453	targeted	Absent	Sample	Upin_Bot2011_COI	GD15	COI	45	BB93453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD15	CTTTAATTACAGCGTTTTTATTGTTGT	151.24058
BB93454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82217	WGS84	BB93454	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93454_Mgig_Bot2012_COI	GD20	1	BB93454_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93454	Sample	BB93454	targeted	Absent	Sample	Mgig_Bot2012_COI	GD20	COI	45	BB93454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD20	TCTTATTCGTTGGAGACTTTATAACCCT	151.24371
BB93455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82269	WGS84	BB93455	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93455_Cmae_Bot2011_COI	GD25	1	BB93455_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93455	Sample	BB93455	targeted	Absent	Sample	Cmae_Bot2011_COI	GD25	COI	45	BB93455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD25	ATGAACAGTCTATCCTCCTTTAG	151.24141
BB93455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82269	WGS84	BB93455	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93455_Msal_Bot2012_COI	GD25	1	BB93455_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93455	Sample	BB93455	targeted	Absent	Sample	Msal_Bot2012_COI	GD25	COI	45	BB93455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD25	GYTAGTTCCRATGATGTTAGCTG	151.24141
BB93457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82366	WGS84	BB93457	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93457_Aamu_Bax2006_COI	GD105	1	BB93457_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93457	Sample	BB93457	targeted	Absent	Sample	Aamu_Bax2006_COI	GD105	COI	45	BB93457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD105	GCACAACCGGGATCTTTACTTC	151.24798
BB93457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82366	WGS84	BB93457	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93457_Pcan_Bot2011_IGS	GD105	1	BB93457_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93457	Sample	BB93457	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD105	IGS	45	BB93457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD105	CGTAATCCTCAGTACTGGCTA	151.24798
BB93457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82366	WGS84	BB93457	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93457_Sspa_Oph2007_COI	GD105	1	BB93457_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93457	Sample	BB93457	targeted	Absent	Sample	Sspa_Oph2007_COI	GD105	COI	45	BB93457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD105	GAAGGCCTTCCGATGATC	151.24798
BB93457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82366	WGS84	BB93457	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93457_Upin_Bot2011_COI	GD105	1	BB93457_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93457	Sample	BB93457	targeted	Absent	Sample	Upin_Bot2011_COI	GD105	COI	45	BB93457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD105	CTTTAATTACAGCGTTTTTATTGTTGT	151.24798
BB93458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82097	WGS84	BB93458	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93458_Mgig_Bot2012_COI	GD110	1	BB93458_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93458	Sample	BB93458	targeted	Absent	Sample	Mgig_Bot2012_COI	GD110	COI	45	BB93458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD110	TCTTATTCGTTGGAGACTTTATAACCCT	151.24484
BB93459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83496	WGS84	BB93459	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93459_Cmae_Bot2011_COI	GD40	1	BB93459_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93459	Sample	BB93459	targeted	Absent	Sample	Cmae_Bot2011_COI	GD40	COI	45	BB93459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD40	ATGAACAGTCTATCCTCCTTTAG	151.26835
BB93459	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83496	WGS84	BB93459	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93459_Msal_Bot2012_COI	GD40	1	BB93459_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93459	Sample	BB93459	targeted	Absent	Sample	Msal_Bot2012_COI	GD40	COI	45	BB93459	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD40	GYTAGTTCCRATGATGTTAGCTG	151.26835
BB93460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83723	WGS84	BB93460	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93460_Aamu_Bax2006_COI	GD100	1	BB93460_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93460	Sample	BB93460	targeted	Absent	Sample	Aamu_Bax2006_COI	GD100	COI	45	BB93460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD100	GCACAACCGGGATCTTTACTTC	151.27246
BB93460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83723	WGS84	BB93460	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93460_Pcan_Bot2011_IGS	GD100	1	BB93460_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93460	Sample	BB93460	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD100	IGS	45	BB93460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD100	CGTAATCCTCAGTACTGGCTA	151.27246
BB93460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83723	WGS84	BB93460	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93460_Sspa_Oph2007_COI	GD100	1	BB93460_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93460	Sample	BB93460	targeted	Absent	Sample	Sspa_Oph2007_COI	GD100	COI	45	BB93460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD100	GAAGGCCTTCCGATGATC	151.27246
BB93460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.83723	WGS84	BB93460	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93460_Upin_Bot2011_COI	GD100	1	BB93460_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93460	Sample	BB93460	targeted	Absent	Sample	Upin_Bot2011_COI	GD100	COI	45	BB93460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD100	CTTTAATTACAGCGTTTTTATTGTTGT	151.27246
BB93461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.80734	WGS84	BB93461	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93461_Mgig_Bot2012_COI	GD45	1	BB93461_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93461	Sample	BB93461	targeted	Absent	Sample	Mgig_Bot2012_COI	GD45	COI	45	BB93461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD45	TCTTATTCGTTGGAGACTTTATAACCCT	151.21889
BB93462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82706	WGS84	BB93462	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93462_Cmae_Bot2011_COI	GD1	1	BB93462_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93462	Sample	BB93462	targeted	Absent	Sample	Cmae_Bot2011_COI	GD1	COI	45	BB93462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD1	ATGAACAGTCTATCCTCCTTTAG	151.24741
BB93462	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82706	WGS84	BB93462	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93462_Msal_Bot2012_COI	GD1	1	BB93462_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93462	Sample	BB93462	targeted	Absent	Sample	Msal_Bot2012_COI	GD1	COI	45	BB93462	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD1	GYTAGTTCCRATGATGTTAGCTG	151.24741
BB93464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81622	WGS84	BB93464	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93464_Aamu_Bax2006_COI	GD3	1	BB93464_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93464	Sample	BB93464	targeted	Absent	Sample	Aamu_Bax2006_COI	GD3	COI	45	BB93464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD3	GCACAACCGGGATCTTTACTTC	151.23706
BB93464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81622	WGS84	BB93464	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93464_Pcan_Bot2011_IGS	GD3	1	BB93464_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93464	Sample	BB93464	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD3	IGS	45	BB93464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD3	CGTAATCCTCAGTACTGGCTA	151.23706
BB93464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81622	WGS84	BB93464	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93464_Sspa_Oph2007_COI	GD3	1	BB93464_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93464	Sample	BB93464	targeted	Absent	Sample	Sspa_Oph2007_COI	GD3	COI	45	BB93464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD3	GAAGGCCTTCCGATGATC	151.23706
BB93464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81622	WGS84	BB93464	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93464_Upin_Bot2011_COI	GD3	1	BB93464_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93464	Sample	BB93464	targeted	Absent	Sample	Upin_Bot2011_COI	GD3	COI	45	BB93464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD3	CTTTAATTACAGCGTTTTTATTGTTGT	151.23706
BB93465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.81546	WGS84	BB93465	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93465_Mgig_Bot2012_COI	GD4	1	BB93465_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93465	Sample	BB93465	targeted	Absent	Sample	Mgig_Bot2012_COI	GD4	COI	45	BB93465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD4	TCTTATTCGTTGGAGACTTTATAACCCT	151.23717
BB93466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8178	WGS84	BB93466	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93466_Cmae_Bot2011_COI	GD7	1	BB93466_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93466	Sample	BB93466	targeted	Absent	Sample	Cmae_Bot2011_COI	GD7	COI	45	BB93466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD7	ATGAACAGTCTATCCTCCTTTAG	151.23909
BB93466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.8178	WGS84	BB93466	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93466_Msal_Bot2012_COI	GD7	1	BB93466_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93466	Sample	BB93466	targeted	Absent	Sample	Msal_Bot2012_COI	GD7	COI	45	BB93466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD7	GYTAGTTCCRATGATGTTAGCTG	151.23909
BB93468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82046	WGS84	BB93468	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93468_Aamu_Bax2006_COI	GD12	1	BB93468_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93468	Sample	BB93468	targeted	Absent	Sample	Aamu_Bax2006_COI	GD12	COI	45	BB93468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD12	GCACAACCGGGATCTTTACTTC	151.23915
BB93468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82046	WGS84	BB93468	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93468_Pcan_Bot2011_IGS	GD12	1	BB93468_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93468	Sample	BB93468	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD12	IGS	45	BB93468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD12	CGTAATCCTCAGTACTGGCTA	151.23915
BB93468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82046	WGS84	BB93468	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93468_Sspa_Oph2007_COI	GD12	1	BB93468_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93468	Sample	BB93468	targeted	Absent	Sample	Sspa_Oph2007_COI	GD12	COI	45	BB93468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD12	GAAGGCCTTCCGATGATC	151.23915
BB93468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82046	WGS84	BB93468	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93468_Upin_Bot2011_COI	GD12	1	BB93468_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93468	Sample	BB93468	targeted	Absent	Sample	Upin_Bot2011_COI	GD12	COI	45	BB93468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD12	CTTTAATTACAGCGTTTTTATTGTTGT	151.23915
BB93469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82166	WGS84	BB93469	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93469_Mgig_Bot2012_COI	GD14	1	BB93469_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93469	Sample	BB93469	targeted	Absent	Sample	Mgig_Bot2012_COI	GD14	COI	45	BB93469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD14	TCTTATTCGTTGGAGACTTTATAACCCT	151.24024
BB93471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82143	WGS84	BB93471	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93471_Aamu_Bax2006_COI	GD19	1	BB93471_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93471	Sample	BB93471	targeted	Absent	Sample	Aamu_Bax2006_COI	GD19	COI	45	BB93471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD19	GCACAACCGGGATCTTTACTTC	151.24285
BB93471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82143	WGS84	BB93471	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93471_Pcan_Bot2011_IGS	GD19	1	BB93471_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93471	Sample	BB93471	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD19	IGS	45	BB93471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD19	CGTAATCCTCAGTACTGGCTA	151.24285
BB93471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82143	WGS84	BB93471	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93471_Sspa_Oph2007_COI	GD19	1	BB93471_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93471	Sample	BB93471	targeted	Absent	Sample	Sspa_Oph2007_COI	GD19	COI	45	BB93471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD19	GAAGGCCTTCCGATGATC	151.24285
BB93471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82143	WGS84	BB93471	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93471_Upin_Bot2011_COI	GD19	1	BB93471_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93471	Sample	BB93471	targeted	Absent	Sample	Upin_Bot2011_COI	GD19	COI	45	BB93471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD19	CTTTAATTACAGCGTTTTTATTGTTGT	151.24285
BB93472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82256	WGS84	BB93472	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93472_Mgig_Bot2012_COI	GD21	1	BB93472_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93472	Sample	BB93472	targeted	Absent	Sample	Mgig_Bot2012_COI	GD21	COI	45	BB93472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD21	TCTTATTCGTTGGAGACTTTATAACCCT	151.24253
BB93473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82382	WGS84	BB93473	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93473_Cmae_Bot2011_COI	GD27	1	BB93473_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93473	Sample	BB93473	targeted	Absent	Sample	Cmae_Bot2011_COI	GD27	COI	45	BB93473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD27	ATGAACAGTCTATCCTCCTTTAG	151.24513
BB93473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82382	WGS84	BB93473	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93473_Msal_Bot2012_COI	GD27	1	BB93473_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93473	Sample	BB93473	targeted	Absent	Sample	Msal_Bot2012_COI	GD27	COI	45	BB93473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD27	GYTAGTTCCRATGATGTTAGCTG	151.24513
BB93475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82536	WGS84	BB93475	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93475_Aamu_Bax2006_COI	GD32	1	BB93475_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93475	Sample	BB93475	targeted	Absent	Sample	Aamu_Bax2006_COI	GD32	COI	45	BB93475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD32	GCACAACCGGGATCTTTACTTC	151.24636
BB93475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82536	WGS84	BB93475	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93475_Pcan_Bot2011_IGS	GD32	1	BB93475_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93475	Sample	BB93475	targeted	Absent	Sample	Pcan_Bot2011_IGS	GD32	IGS	45	BB93475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD32	CGTAATCCTCAGTACTGGCTA	151.24636
BB93475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82536	WGS84	BB93475	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93475_Sspa_Oph2007_COI	GD32	1	BB93475_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93475	Sample	BB93475	targeted	Absent	Sample	Sspa_Oph2007_COI	GD32	COI	45	BB93475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD32	GAAGGCCTTCCGATGATC	151.24636
BB93475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82536	WGS84	BB93475	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93475_Upin_Bot2011_COI	GD32	1	BB93475_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93475	Sample	BB93475	targeted	Absent	Sample	Upin_Bot2011_COI	GD32	COI	45	BB93475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD32	CTTTAATTACAGCGTTTTTATTGTTGT	151.24636
BB93476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-23.82527	WGS84	BB93476	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93476_Mgig_Bot2012_COI	GD33	1	BB93476_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93476	Sample	BB93476	targeted	Absent	Sample	Mgig_Bot2012_COI	GD33	COI	45	BB93476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD33	TCTTATTCGTTGGAGACTTTATAACCCT	151.24526
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93477_Cmae_Bot2011_COI	MD125	1	BB93477_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Absent	Sample	Cmae_Bot2011_COI	MD125	COI	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	ATGAACAGTCTATCCTCCTTTAG	144.90157
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93477_Hsan_Wil2021_COI	MD125	1	BB93477_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Absent	Sample	Hsan_Wil2021_COI	MD125	COI	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	CCTGGGCCGGTATAGTAGGT	144.90157
BB93477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82676	WGS84	BB93477	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93477_Msal_Bot2012_COI	MD125	1	BB93477_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93477	Sample	BB93477	targeted	Absent	Sample	Msal_Bot2012_COI	MD125	COI	45	BB93477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD125	GYTAGTTCCRATGATGTTAGCTG	144.90157
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.162	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93479_Aamu_Bax2006_COI	MD115	1	BB93479_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Present	Sample	Aamu_Bax2006_COI	MD115	COI	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	GCACAACCGGGATCTTTACTTC	144.90722
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93479_Pcan_Bot2011_IGS	MD115	1	BB93479_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD115	IGS	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	CGTAATCCTCAGTACTGGCTA	144.90722
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.871	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93479_Sspa_Oph2007_COI	MD115	1	BB93479_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Present	Sample	Sspa_Oph2007_COI	MD115	COI	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	GAAGGCCTTCCGATGATC	144.90722
BB93479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82238	WGS84	BB93479	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93479_Upin_Bot2011_COI	MD115	1	BB93479_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93479	Sample	BB93479	targeted	Absent	Sample	Upin_Bot2011_COI	MD115	COI	45	BB93479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD115	CTTTAATTACAGCGTTTTTATTGTTGT	144.90722
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93480_Cmae_Bot2011_COI	MD105	1	BB93480_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Absent	Sample	Cmae_Bot2011_COI	MD105	COI	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	ATGAACAGTCTATCCTCCTTTAG	144.91047
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93480_Hsan_Wil2021_COI	MD105	1	BB93480_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Absent	Sample	Hsan_Wil2021_COI	MD105	COI	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	CCTGGGCCGGTATAGTAGGT	144.91047
BB93480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82125	WGS84	BB93480	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93480_Msal_Bot2012_COI	MD105	1	BB93480_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93480	Sample	BB93480	targeted	Absent	Sample	Msal_Bot2012_COI	MD105	COI	45	BB93480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD105	GYTAGTTCCRATGATGTTAGCTG	144.91047
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.091	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93482_Aamu_Bax2006_COI	MD90	1	BB93482_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Present	Sample	Aamu_Bax2006_COI	MD90	COI	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	GCACAACCGGGATCTTTACTTC	144.91436
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93482_Pcan_Bot2011_IGS	MD90	1	BB93482_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD90	IGS	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	CGTAATCCTCAGTACTGGCTA	144.91436
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.762	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93482_Sspa_Oph2007_COI	MD90	1	BB93482_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Present	Sample	Sspa_Oph2007_COI	MD90	COI	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	GAAGGCCTTCCGATGATC	144.91436
BB93482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81416	WGS84	BB93482	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93482_Upin_Bot2011_COI	MD90	1	BB93482_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93482	Sample	BB93482	targeted	Absent	Sample	Upin_Bot2011_COI	MD90	COI	45	BB93482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD90	CTTTAATTACAGCGTTTTTATTGTTGT	144.91436
BB93483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81802	WGS84	BB93483	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93483_Mgig_Bot2012_COI	MD85	1	BB93483_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93483	Sample	BB93483	targeted	Absent	Sample	Mgig_Bot2012_COI	MD85	COI	45	BB93483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD85	TCTTATTCGTTGGAGACTTTATAACCCT	144.91266
BB93484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81957	WGS84	BB93484	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93484_Cmae_Bot2011_COI	MD80	1	BB93484_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93484	Sample	BB93484	targeted	Absent	Sample	Cmae_Bot2011_COI	MD80	COI	45	BB93484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD80	ATGAACAGTCTATCCTCCTTTAG	144.91825
BB93484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81957	WGS84	BB93484	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93484_Msal_Bot2012_COI	MD80	1	BB93484_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93484	Sample	BB93484	targeted	Absent	Sample	Msal_Bot2012_COI	MD80	COI	45	BB93484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD80	GYTAGTTCCRATGATGTTAGCTG	144.91825
BB93486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81858	WGS84	BB93486	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.103	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93486_Aamu_Bax2006_COI	MD65	1	BB93486_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93486	Sample	BB93486	targeted	Present	Sample	Aamu_Bax2006_COI	MD65	COI	45	BB93486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD65	GCACAACCGGGATCTTTACTTC	144.92668
BB93486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81858	WGS84	BB93486	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93486_Pcan_Bot2011_IGS	MD65	1	BB93486_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93486	Sample	BB93486	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD65	IGS	45	BB93486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD65	CGTAATCCTCAGTACTGGCTA	144.92668
BB93486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81858	WGS84	BB93486	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.358	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93486_Sspa_Oph2007_COI	MD65	1	BB93486_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93486	Sample	BB93486	targeted	Present	Sample	Sspa_Oph2007_COI	MD65	COI	45	BB93486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD65	GAAGGCCTTCCGATGATC	144.92668
BB93486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81858	WGS84	BB93486	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93486_Upin_Bot2011_COI	MD65	1	BB93486_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93486	Sample	BB93486	targeted	Absent	Sample	Upin_Bot2011_COI	MD65	COI	45	BB93486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD65	CTTTAATTACAGCGTTTTTATTGTTGT	144.92668
BB93487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8189	WGS84	BB93487	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93487_Mgig_Bot2012_COI	MD60	1	BB93487_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93487	Sample	BB93487	targeted	Absent	Sample	Mgig_Bot2012_COI	MD60	COI	45	BB93487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD60	TCTTATTCGTTGGAGACTTTATAACCCT	144.92881
BB93488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86098	WGS84	BB93488	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93488_Cmae_Bot2011_COI	MD55	1	BB93488_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93488	Sample	BB93488	targeted	Absent	Sample	Cmae_Bot2011_COI	MD55	COI	45	BB93488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD55	ATGAACAGTCTATCCTCCTTTAG	144.90725
BB93488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86098	WGS84	BB93488	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93488_Msal_Bot2012_COI	MD55	1	BB93488_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93488	Sample	BB93488	targeted	Absent	Sample	Msal_Bot2012_COI	MD55	COI	45	BB93488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD55	GYTAGTTCCRATGATGTTAGCTG	144.90725
BB93490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93490	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93490_Mgig_Bot2012_COI	MD35	1	BB93490_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93490	Sample	BB93490	targeted	Absent	Sample	Mgig_Bot2012_COI	MD35	COI	45	BB93490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD35	TCTTATTCGTTGGAGACTTTATAACCCT	144.90751
BB93491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86484	WGS84	BB93491	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93491_Cmae_Bot2011_COI	MD30	1	BB93491_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93491	Sample	BB93491	targeted	Absent	Sample	Cmae_Bot2011_COI	MD30	COI	45	BB93491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD30	ATGAACAGTCTATCCTCCTTTAG	144.91759
BB93491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86484	WGS84	BB93491	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93491_Msal_Bot2012_COI	MD30	1	BB93491_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93491	Sample	BB93491	targeted	Absent	Sample	Msal_Bot2012_COI	MD30	COI	45	BB93491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD30	GYTAGTTCCRATGATGTTAGCTG	144.91759
BB93493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82706	WGS84	BB93493	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.206	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93493_Aamu_Bax2006_COI	MD124	1	BB93493_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93493	Sample	BB93493	targeted	Present	Sample	Aamu_Bax2006_COI	MD124	COI	45	BB93493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD124	GCACAACCGGGATCTTTACTTC	144.89997
BB93493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82706	WGS84	BB93493	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93493_Pcan_Bot2011_IGS	MD124	1	BB93493_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93493	Sample	BB93493	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD124	IGS	45	BB93493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD124	CGTAATCCTCAGTACTGGCTA	144.89997
BB93493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82706	WGS84	BB93493	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.593	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93493_Sspa_Oph2007_COI	MD124	1	BB93493_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93493	Sample	BB93493	targeted	Present	Sample	Sspa_Oph2007_COI	MD124	COI	45	BB93493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD124	GAAGGCCTTCCGATGATC	144.89997
BB93493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82706	WGS84	BB93493	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93493_Upin_Bot2011_COI	MD124	1	BB93493_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93493	Sample	BB93493	targeted	Absent	Sample	Upin_Bot2011_COI	MD124	COI	45	BB93493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD124	CTTTAATTACAGCGTTTTTATTGTTGT	144.89997
BB93494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82574	WGS84	BB93494	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93494_Mgig_Bot2012_COI	MD123	1	BB93494_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93494	Sample	BB93494	targeted	Absent	Sample	Mgig_Bot2012_COI	MD123	COI	45	BB93494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD123	TCTTATTCGTTGGAGACTTTATAACCCT	144.90211
BB93495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8245	WGS84	BB93495	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93495_Cmae_Bot2011_COI	MD119	1	BB93495_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93495	Sample	BB93495	targeted	Absent	Sample	Cmae_Bot2011_COI	MD119	COI	45	BB93495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD119	ATGAACAGTCTATCCTCCTTTAG	144.904
BB93495	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8245	WGS84	BB93495	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93495_Msal_Bot2012_COI	MD119	1	BB93495_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93495	Sample	BB93495	targeted	Absent	Sample	Msal_Bot2012_COI	MD119	COI	45	BB93495	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD119	GYTAGTTCCRATGATGTTAGCTG	144.904
BB93497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8209	WGS84	BB93497	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.111	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93497_Aamu_Bax2006_COI	MD113	1	BB93497_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93497	Sample	BB93497	targeted	Present	Sample	Aamu_Bax2006_COI	MD113	COI	45	BB93497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD113	GCACAACCGGGATCTTTACTTC	144.90658
BB93497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8209	WGS84	BB93497	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93497_Pcan_Bot2011_IGS	MD113	1	BB93497_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93497	Sample	BB93497	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD113	IGS	45	BB93497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD113	CGTAATCCTCAGTACTGGCTA	144.90658
BB93497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8209	WGS84	BB93497	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.119	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93497_Sspa_Oph2007_COI	MD113	1	BB93497_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93497	Sample	BB93497	targeted	Present	Sample	Sspa_Oph2007_COI	MD113	COI	45	BB93497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD113	GAAGGCCTTCCGATGATC	144.90658
BB93497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8209	WGS84	BB93497	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93497_Upin_Bot2011_COI	MD113	1	BB93497_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93497	Sample	BB93497	targeted	Absent	Sample	Upin_Bot2011_COI	MD113	COI	45	BB93497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD113	CTTTAATTACAGCGTTTTTATTGTTGT	144.90658
BB93498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81641	WGS84	BB93498	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93498_Mgig_Bot2012_COI	MD111	1	BB93498_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93498	Sample	BB93498	targeted	Absent	Sample	Mgig_Bot2012_COI	MD111	COI	45	BB93498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD111	TCTTATTCGTTGGAGACTTTATAACCCT	144.90597
BB93499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82141	WGS84	BB93499	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93499_Cmae_Bot2011_COI	MD108	1	BB93499_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93499	Sample	BB93499	targeted	Absent	Sample	Cmae_Bot2011_COI	MD108	COI	45	BB93499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD108	ATGAACAGTCTATCCTCCTTTAG	144.90788
BB93499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82141	WGS84	BB93499	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93499_Msal_Bot2012_COI	MD108	1	BB93499_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93499	Sample	BB93499	targeted	Absent	Sample	Msal_Bot2012_COI	MD108	COI	45	BB93499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD108	GYTAGTTCCRATGATGTTAGCTG	144.90788
BB93501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82138	WGS84	BB93501	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.648	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93501_Aamu_Bax2006_COI	MD106	1	BB93501_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93501	Sample	BB93501	targeted	Present	Sample	Aamu_Bax2006_COI	MD106	COI	45	BB93501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD106	GCACAACCGGGATCTTTACTTC	144.90934
BB93501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82138	WGS84	BB93501	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93501_Pcan_Bot2011_IGS	MD106	1	BB93501_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93501	Sample	BB93501	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD106	IGS	45	BB93501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD106	CGTAATCCTCAGTACTGGCTA	144.90934
BB93501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82138	WGS84	BB93501	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.252	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93501_Sspa_Oph2007_COI	MD106	1	BB93501_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93501	Sample	BB93501	targeted	Present	Sample	Sspa_Oph2007_COI	MD106	COI	45	BB93501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD106	GAAGGCCTTCCGATGATC	144.90934
BB93501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82138	WGS84	BB93501	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93501_Upin_Bot2011_COI	MD106	1	BB93501_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93501	Sample	BB93501	targeted	Absent	Sample	Upin_Bot2011_COI	MD106	COI	45	BB93501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD106	CTTTAATTACAGCGTTTTTATTGTTGT	144.90934
BB93502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82112	WGS84	BB93502	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93502_Mgig_Bot2012_COI	MD104	1	BB93502_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93502	Sample	BB93502	targeted	Absent	Sample	Mgig_Bot2012_COI	MD104	COI	45	BB93502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD104	TCTTATTCGTTGGAGACTTTATAACCCT	144.91172
BB93503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8201	WGS84	BB93503	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93503_Cmae_Bot2011_COI	MD101	1	BB93503_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93503	Sample	BB93503	targeted	Absent	Sample	Cmae_Bot2011_COI	MD101	COI	45	BB93503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD101	ATGAACAGTCTATCCTCCTTTAG	144.91155
BB93503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8201	WGS84	BB93503	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93503_Msal_Bot2012_COI	MD101	1	BB93503_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93503	Sample	BB93503	targeted	Absent	Sample	Msal_Bot2012_COI	MD101	COI	45	BB93503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD101	GYTAGTTCCRATGATGTTAGCTG	144.91155
BB93505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81014	WGS84	BB93505	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.661	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93505_Aamu_Bax2006_COI	MD96	1	BB93505_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93505	Sample	BB93505	targeted	Present	Sample	Aamu_Bax2006_COI	MD96	COI	45	BB93505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD96	GCACAACCGGGATCTTTACTTC	144.91453
BB93505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81014	WGS84	BB93505	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93505_Pcan_Bot2011_IGS	MD96	1	BB93505_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93505	Sample	BB93505	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD96	IGS	45	BB93505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD96	CGTAATCCTCAGTACTGGCTA	144.91453
BB93505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81014	WGS84	BB93505	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.678	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93505_Sspa_Oph2007_COI	MD96	1	BB93505_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93505	Sample	BB93505	targeted	Present	Sample	Sspa_Oph2007_COI	MD96	COI	45	BB93505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD96	GAAGGCCTTCCGATGATC	144.91453
BB93505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81014	WGS84	BB93505	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93505_Upin_Bot2011_COI	MD96	1	BB93505_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93505	Sample	BB93505	targeted	Absent	Sample	Upin_Bot2011_COI	MD96	COI	45	BB93505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD96	CTTTAATTACAGCGTTTTTATTGTTGT	144.91453
BB93506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81156	WGS84	BB93506	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93506_Mgig_Bot2012_COI	MD94	1	BB93506_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93506	Sample	BB93506	targeted	Absent	Sample	Mgig_Bot2012_COI	MD94	COI	45	BB93506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD94	TCTTATTCGTTGGAGACTTTATAACCCT	144.91409
BB93507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81165	WGS84	BB93507	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93507_Cmae_Bot2011_COI	MD93	1	BB93507_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93507	Sample	BB93507	targeted	Absent	Sample	Cmae_Bot2011_COI	MD93	COI	45	BB93507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD93	ATGAACAGTCTATCCTCCTTTAG	144.91297
BB93507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81165	WGS84	BB93507	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93507_Msal_Bot2012_COI	MD93	1	BB93507_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93507	Sample	BB93507	targeted	Absent	Sample	Msal_Bot2012_COI	MD93	COI	45	BB93507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD93	GYTAGTTCCRATGATGTTAGCTG	144.91297
BB93509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81584	WGS84	BB93509	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.462	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93509_Aamu_Bax2006_COI	MD88	1	BB93509_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93509	Sample	BB93509	targeted	Present	Sample	Aamu_Bax2006_COI	MD88	COI	45	BB93509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD88	GCACAACCGGGATCTTTACTTC	144.91397
BB93509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81584	WGS84	BB93509	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93509_Pcan_Bot2011_IGS	MD88	1	BB93509_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93509	Sample	BB93509	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD88	IGS	45	BB93509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD88	CGTAATCCTCAGTACTGGCTA	144.91397
BB93509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81584	WGS84	BB93509	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.684	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93509_Sspa_Oph2007_COI	MD88	1	BB93509_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93509	Sample	BB93509	targeted	Present	Sample	Sspa_Oph2007_COI	MD88	COI	45	BB93509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD88	GAAGGCCTTCCGATGATC	144.91397
BB93509	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81584	WGS84	BB93509	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93509_Upin_Bot2011_COI	MD88	1	BB93509_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93509	Sample	BB93509	targeted	Absent	Sample	Upin_Bot2011_COI	MD88	COI	45	BB93509	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD88	CTTTAATTACAGCGTTTTTATTGTTGT	144.91397
BB93510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81717	WGS84	BB93510	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93510_Cmae_Bot2011_COI	MD86	1	BB93510_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93510	Sample	BB93510	targeted	Absent	Sample	Cmae_Bot2011_COI	MD86	COI	45	BB93510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD86	ATGAACAGTCTATCCTCCTTTAG	144.91329
BB93510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81717	WGS84	BB93510	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93510_Msal_Bot2012_COI	MD86	1	BB93510_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93510	Sample	BB93510	targeted	Absent	Sample	Msal_Bot2012_COI	MD86	COI	45	BB93510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD86	GYTAGTTCCRATGATGTTAGCTG	144.91329
BB93512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81938	WGS84	BB93512	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.88	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93512_Aamu_Bax2006_COI	MD82	1	BB93512_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93512	Sample	BB93512	targeted	Present	Sample	Aamu_Bax2006_COI	MD82	COI	45	BB93512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD82	GCACAACCGGGATCTTTACTTC	144.91696
BB93512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81938	WGS84	BB93512	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93512_Pcan_Bot2011_IGS	MD82	1	BB93512_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93512	Sample	BB93512	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD82	IGS	45	BB93512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD82	CGTAATCCTCAGTACTGGCTA	144.91696
BB93512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81938	WGS84	BB93512	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.71	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93512_Sspa_Oph2007_COI	MD82	1	BB93512_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93512	Sample	BB93512	targeted	Present	Sample	Sspa_Oph2007_COI	MD82	COI	45	BB93512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD82	GAAGGCCTTCCGATGATC	144.91696
BB93512	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81938	WGS84	BB93512	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93512_Upin_Bot2011_COI	MD82	1	BB93512_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93512	Sample	BB93512	targeted	Absent	Sample	Upin_Bot2011_COI	MD82	COI	45	BB93512	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD82	CTTTAATTACAGCGTTTTTATTGTTGT	144.91696
BB93513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81971	WGS84	BB93513	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.071	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93513_Mgig_Bot2012_COI	MD79	1	BB93513_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93513	Sample	BB93513	targeted	Present	Sample	Mgig_Bot2012_COI	MD79	COI	45	BB93513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD79	TCTTATTCGTTGGAGACTTTATAACCCT	144.91962
BB93514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81923	WGS84	BB93514	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93514_Cmae_Bot2011_COI	MD77	1	BB93514_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93514	Sample	BB93514	targeted	Absent	Sample	Cmae_Bot2011_COI	MD77	COI	45	BB93514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD77	ATGAACAGTCTATCCTCCTTTAG	144.92092
BB93514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81923	WGS84	BB93514	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93514_Msal_Bot2012_COI	MD77	1	BB93514_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93514	Sample	BB93514	targeted	Absent	Sample	Msal_Bot2012_COI	MD77	COI	45	BB93514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD77	GYTAGTTCCRATGATGTTAGCTG	144.92092
BB93516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8197	WGS84	BB93516	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.216	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93516_Aamu_Bax2006_COI	MD73	1	BB93516_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93516	Sample	BB93516	targeted	Present	Sample	Aamu_Bax2006_COI	MD73	COI	45	BB93516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD73	GCACAACCGGGATCTTTACTTC	144.92558
BB93516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8197	WGS84	BB93516	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93516_Pcan_Bot2011_IGS	MD73	1	BB93516_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93516	Sample	BB93516	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD73	IGS	45	BB93516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD73	CGTAATCCTCAGTACTGGCTA	144.92558
BB93516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8197	WGS84	BB93516	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.38	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93516_Sspa_Oph2007_COI	MD73	1	BB93516_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93516	Sample	BB93516	targeted	Present	Sample	Sspa_Oph2007_COI	MD73	COI	45	BB93516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD73	GAAGGCCTTCCGATGATC	144.92558
BB93516	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8197	WGS84	BB93516	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93516_Upin_Bot2011_COI	MD73	1	BB93516_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93516	Sample	BB93516	targeted	Absent	Sample	Upin_Bot2011_COI	MD73	COI	45	BB93516	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD73	CTTTAATTACAGCGTTTTTATTGTTGT	144.92558
BB93517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81825	WGS84	BB93517	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93517_Mgig_Bot2012_COI	MD71	1	BB93517_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93517	Sample	BB93517	targeted	Absent	Sample	Mgig_Bot2012_COI	MD71	COI	45	BB93517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD71	TCTTATTCGTTGGAGACTTTATAACCCT	144.92325
BB93518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81672	WGS84	BB93518	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93518_Cmae_Bot2011_COI	MD68	1	BB93518_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93518	Sample	BB93518	targeted	Absent	Sample	Cmae_Bot2011_COI	MD68	COI	45	BB93518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD68	ATGAACAGTCTATCCTCCTTTAG	144.92453
BB93518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81672	WGS84	BB93518	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93518_Msal_Bot2012_COI	MD68	1	BB93518_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93518	Sample	BB93518	targeted	Absent	Sample	Msal_Bot2012_COI	MD68	COI	45	BB93518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD68	GYTAGTTCCRATGATGTTAGCTG	144.92453
BB93520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.81935	WGS84	BB93520	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.782	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93520_Mgig_Bot2012_COI	MD63	1	BB93520_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93520	Sample	BB93520	targeted	Present	Sample	Mgig_Bot2012_COI	MD63	COI	45	BB93520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD63	TCTTATTCGTTGGAGACTTTATAACCCT	144.92706
BB93521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82012	WGS84	BB93521	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93521_Cmae_Bot2011_COI	MD59	1	BB93521_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93521	Sample	BB93521	targeted	Absent	Sample	Cmae_Bot2011_COI	MD59	COI	45	BB93521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD59	ATGAACAGTCTATCCTCCTTTAG	144.92885
BB93521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.82012	WGS84	BB93521	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93521_Msal_Bot2012_COI	MD59	1	BB93521_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93521	Sample	BB93521	targeted	Absent	Sample	Msal_Bot2012_COI	MD59	COI	45	BB93521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD59	GYTAGTTCCRATGATGTTAGCTG	144.92885
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.729	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93523_Aamu_Bax2006_COI	MD53	1	BB93523_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Present	Sample	Aamu_Bax2006_COI	MD53	COI	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	GCACAACCGGGATCTTTACTTC	144.91028
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93523_Pcan_Bot2011_IGS	MD53	1	BB93523_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD53	IGS	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	CGTAATCCTCAGTACTGGCTA	144.91028
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.777	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93523_Sspa_Oph2007_COI	MD53	1	BB93523_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Present	Sample	Sspa_Oph2007_COI	MD53	COI	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	GAAGGCCTTCCGATGATC	144.91028
BB93523	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86028	WGS84	BB93523	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.683	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93523_Upin_Bot2011_COI	MD53	1	BB93523_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93523	Sample	BB93523	targeted	Present	Sample	Upin_Bot2011_COI	MD53	COI	45	BB93523	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD53	CTTTAATTACAGCGTTTTTATTGTTGT	144.91028
BB93524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86133	WGS84	BB93524	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93524_Mgig_Bot2012_COI	MD51	1	BB93524_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93524	Sample	BB93524	targeted	Absent	Sample	Mgig_Bot2012_COI	MD51	COI	45	BB93524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD51	TCTTATTCGTTGGAGACTTTATAACCCT	144.91089
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93525_Cmae_Bot2011_COI	MD49	1	BB93525_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Absent	Sample	Cmae_Bot2011_COI	MD49	COI	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	ATGAACAGTCTATCCTCCTTTAG	144.91331
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93525_Hsan_Wil2021_COI	MD49	1	BB93525_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Absent	Sample	Hsan_Wil2021_COI	MD49	COI	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	CCTGGGCCGGTATAGTAGGT	144.91331
BB93525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85976	WGS84	BB93525	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93525_Msal_Bot2012_COI	MD49	1	BB93525_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93525	Sample	BB93525	targeted	Absent	Sample	Msal_Bot2012_COI	MD49	COI	45	BB93525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD49	GYTAGTTCCRATGATGTTAGCTG	144.91331
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.797	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93527_Aamu_Bax2006_COI	MD44	1	BB93527_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Present	Sample	Aamu_Bax2006_COI	MD44	COI	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	GCACAACCGGGATCTTTACTTC	144.91391
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93527_Pcan_Bot2011_IGS	MD44	1	BB93527_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD44	IGS	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	CGTAATCCTCAGTACTGGCTA	144.91391
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.162	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93527_Sspa_Oph2007_COI	MD44	1	BB93527_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Present	Sample	Sspa_Oph2007_COI	MD44	COI	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	GAAGGCCTTCCGATGATC	144.91391
BB93527	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86288	WGS84	BB93527	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.076	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93527_Upin_Bot2011_COI	MD44	1	BB93527_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93527	Sample	BB93527	targeted	Present	Sample	Upin_Bot2011_COI	MD44	COI	45	BB93527	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD44	CTTTAATTACAGCGTTTTTATTGTTGT	144.91391
BB93528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86147	WGS84	BB93528	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93528_Mgig_Bot2012_COI	MD43	1	BB93528_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93528	Sample	BB93528	targeted	Absent	Sample	Mgig_Bot2012_COI	MD43	COI	45	BB93528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD43	TCTTATTCGTTGGAGACTTTATAACCCT	144.91599
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.758	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93529_Cmae_Bot2011_COI	MD39	1	BB93529_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Present	Sample	Cmae_Bot2011_COI	MD39	COI	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	ATGAACAGTCTATCCTCCTTTAG	144.91853
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93529_Hsan_Wil2021_COI	MD39	1	BB93529_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Absent	Sample	Hsan_Wil2021_COI	MD39	COI	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	CCTGGGCCGGTATAGTAGGT	144.91853
BB93529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.8639	WGS84	BB93529	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93529_Msal_Bot2012_COI	MD39	1	BB93529_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93529	Sample	BB93529	targeted	Absent	Sample	Msal_Bot2012_COI	MD39	COI	45	BB93529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD39	GYTAGTTCCRATGATGTTAGCTG	144.91853
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.243	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93530_Aamu_Bax2006_COI	MD38	1	BB93530_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Present	Sample	Aamu_Bax2006_COI	MD38	COI	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	GCACAACCGGGATCTTTACTTC	144.91628
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93530_Pcan_Bot2011_IGS	MD38	1	BB93530_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD38	IGS	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	CGTAATCCTCAGTACTGGCTA	144.91628
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.624	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93530_Sspa_Oph2007_COI	MD38	1	BB93530_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Present	Sample	Sspa_Oph2007_COI	MD38	COI	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	GAAGGCCTTCCGATGATC	144.91628
BB93530	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.86356	WGS84	BB93530	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.113	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93530_Upin_Bot2011_COI	MD38	1	BB93530_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93530	Sample	BB93530	targeted	Present	Sample	Upin_Bot2011_COI	MD38	COI	45	BB93530	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD38	CTTTAATTACAGCGTTTTTATTGTTGT	144.91628
BB93531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85783	WGS84	BB93531	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93531_Mgig_Bot2012_COI	MD37	1	BB93531_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93531	Sample	BB93531	targeted	Absent	Sample	Mgig_Bot2012_COI	MD37	COI	45	BB93531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD37	TCTTATTCGTTGGAGACTTTATAACCCT	144.9044
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93532_Cmae_Bot2011_COI	MD36	1	BB93532_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Absent	Sample	Cmae_Bot2011_COI	MD36	COI	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	ATGAACAGTCTATCCTCCTTTAG	144.90613
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93532_Hsan_Wil2021_COI	MD36	1	BB93532_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Absent	Sample	Hsan_Wil2021_COI	MD36	COI	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	CCTGGGCCGGTATAGTAGGT	144.90613
BB93532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.85842	WGS84	BB93532	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93532_Msal_Bot2012_COI	MD36	1	BB93532_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93532	Sample	BB93532	targeted	Absent	Sample	Msal_Bot2012_COI	MD36	COI	45	BB93532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD36	GYTAGTTCCRATGATGTTAGCTG	144.90613
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.689	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93534_Aamu_Bax2006_COI	MD28	1	BB93534_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Present	Sample	Aamu_Bax2006_COI	MD28	COI	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	GCACAACCGGGATCTTTACTTC	144.9158
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93534_Pcan_Bot2011_IGS	MD28	1	BB93534_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD28	IGS	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	CGTAATCCTCAGTACTGGCTA	144.9158
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.158	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93534_Sspa_Oph2007_COI	MD28	1	BB93534_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Present	Sample	Sspa_Oph2007_COI	MD28	COI	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	GAAGGCCTTCCGATGATC	144.9158
BB93534	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84942	WGS84	BB93534	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.746	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93534_Upin_Bot2011_COI	MD28	1	BB93534_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93534	Sample	BB93534	targeted	Present	Sample	Upin_Bot2011_COI	MD28	COI	45	BB93534	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD28	CTTTAATTACAGCGTTTTTATTGTTGT	144.9158
BB93535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84996	WGS84	BB93535	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.743	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93535_Mgig_Bot2012_COI	MD26	1	BB93535_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93535	Sample	BB93535	targeted	Present	Sample	Mgig_Bot2012_COI	MD26	COI	45	BB93535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD26	TCTTATTCGTTGGAGACTTTATAACCCT	144.91145
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93536_Cmae_Bot2011_COI	MD23	1	BB93536_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Absent	Sample	Cmae_Bot2011_COI	MD23	COI	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	ATGAACAGTCTATCCTCCTTTAG	144.90969
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93536_Hsan_Wil2021_COI	MD23	1	BB93536_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Absent	Sample	Hsan_Wil2021_COI	MD23	COI	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	CCTGGGCCGGTATAGTAGGT	144.90969
BB93536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84824	WGS84	BB93536	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93536_Msal_Bot2012_COI	MD23	1	BB93536_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93536	Sample	BB93536	targeted	Absent	Sample	Msal_Bot2012_COI	MD23	COI	45	BB93536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD23	GYTAGTTCCRATGATGTTAGCTG	144.90969
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.984	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93538_Aamu_Bax2006_COI	MD19	1	BB93538_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Present	Sample	Aamu_Bax2006_COI	MD19	COI	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	GCACAACCGGGATCTTTACTTC	144.90713
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93538_Pcan_Bot2011_IGS	MD19	1	BB93538_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD19	IGS	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	CGTAATCCTCAGTACTGGCTA	144.90713
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.72	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93538_Sspa_Oph2007_COI	MD19	1	BB93538_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Present	Sample	Sspa_Oph2007_COI	MD19	COI	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	GAAGGCCTTCCGATGATC	144.90713
BB93538	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84746	WGS84	BB93538	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.344	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93538_Upin_Bot2011_COI	MD19	1	BB93538_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93538	Sample	BB93538	targeted	Present	Sample	Upin_Bot2011_COI	MD19	COI	45	BB93538	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD19	CTTTAATTACAGCGTTTTTATTGTTGT	144.90713
BB93539	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84453	WGS84	BB93539	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93539_Mgig_Bot2012_COI	MD17	1	BB93539_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93539	Sample	BB93539	targeted	Absent	Sample	Mgig_Bot2012_COI	MD17	COI	45	BB93539	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD17	TCTTATTCGTTGGAGACTTTATAACCCT	144.90621
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.11	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93541_Aamu_Bax2006_COI	MD14	1	BB93541_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Present	Sample	Aamu_Bax2006_COI	MD14	COI	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	GCACAACCGGGATCTTTACTTC	144.90545
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93541_Pcan_Bot2011_IGS	MD14	1	BB93541_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD14	IGS	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	CGTAATCCTCAGTACTGGCTA	144.90545
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.976	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93541_Sspa_Oph2007_COI	MD14	1	BB93541_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Present	Sample	Sspa_Oph2007_COI	MD14	COI	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	GAAGGCCTTCCGATGATC	144.90545
BB93541	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84163	WGS84	BB93541	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.048	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93541_Upin_Bot2011_COI	MD14	1	BB93541_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93541	Sample	BB93541	targeted	Present	Sample	Upin_Bot2011_COI	MD14	COI	45	BB93541	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD14	CTTTAATTACAGCGTTTTTATTGTTGT	144.90545
BB93542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.83955	WGS84	BB93542	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93542_Mgig_Bot2012_COI	MD12	1	BB93542_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93542	Sample	BB93542	targeted	Absent	Sample	Mgig_Bot2012_COI	MD12	COI	45	BB93542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD12	TCTTATTCGTTGGAGACTTTATAACCCT	144.90411
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93543_Cmae_Bot2011_COI	MD11	1	BB93543_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Absent	Sample	Cmae_Bot2011_COI	MD11	COI	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	ATGAACAGTCTATCCTCCTTTAG	144.92611
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93543_Hsan_Wil2021_COI	MD11	1	BB93543_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Absent	Sample	Hsan_Wil2021_COI	MD11	COI	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	CCTGGGCCGGTATAGTAGGT	144.92611
BB93543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84161	WGS84	BB93543	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93543_Msal_Bot2012_COI	MD11	1	BB93543_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93543	Sample	BB93543	targeted	Absent	Sample	Msal_Bot2012_COI	MD11	COI	45	BB93543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD11	GYTAGTTCCRATGATGTTAGCTG	144.92611
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.828	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93545_Aamu_Bax2006_COI	MD7	1	BB93545_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Present	Sample	Aamu_Bax2006_COI	MD7	COI	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	GCACAACCGGGATCTTTACTTC	144.92959
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93545_Pcan_Bot2011_IGS	MD7	1	BB93545_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Absent	Sample	Pcan_Bot2011_IGS	MD7	IGS	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	CGTAATCCTCAGTACTGGCTA	144.92959
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.845	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93545_Sspa_Oph2007_COI	MD7	1	BB93545_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Present	Sample	Sspa_Oph2007_COI	MD7	COI	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	GAAGGCCTTCCGATGATC	144.92959
BB93545	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84115	WGS84	BB93545	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.365	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93545_Upin_Bot2011_COI	MD7	1	BB93545_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93545	Sample	BB93545	targeted	Present	Sample	Upin_Bot2011_COI	MD7	COI	45	BB93545	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD7	CTTTAATTACAGCGTTTTTATTGTTGT	144.92959
BB93546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84299	WGS84	BB93546	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93546_Mgig_Bot2012_COI	MD6	1	BB93546_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93546	Sample	BB93546	targeted	Absent	Sample	Mgig_Bot2012_COI	MD6	COI	45	BB93546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD6	TCTTATTCGTTGGAGACTTTATAACCCT	144.92993
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.742	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93547_Cmae_Bot2011_COI	MD4	1	BB93547_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Present	Sample	Cmae_Bot2011_COI	MD4	COI	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	ATGAACAGTCTATCCTCCTTTAG	144.92859
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93547_Hsan_Wil2021_COI	MD4	1	BB93547_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Absent	Sample	Hsan_Wil2021_COI	MD4	COI	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	CCTGGGCCGGTATAGTAGGT	144.92859
BB93547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10629	-37.84647	WGS84	BB93547	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93547_Msal_Bot2012_COI	MD4	1	BB93547_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93547	Sample	BB93547	targeted	Absent	Sample	Msal_Bot2012_COI	MD4	COI	45	BB93547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MD4	GYTAGTTCCRATGATGTTAGCTG	144.92859
BB93549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36339	WGS84	BB93549	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93549_Aamu_Bax2006_COI	BD60	1	BB93549_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93549	Sample	BB93549	targeted	Absent	Sample	Aamu_Bax2006_COI	BD60	COI	45	BB93549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD60	GCACAACCGGGATCTTTACTTC	153.17593
BB93549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36339	WGS84	BB93549	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93549_Pcan_Bot2011_IGS	BD60	1	BB93549_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93549	Sample	BB93549	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD60	IGS	45	BB93549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD60	CGTAATCCTCAGTACTGGCTA	153.17593
BB93549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36339	WGS84	BB93549	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93549_Sspa_Oph2007_COI	BD60	1	BB93549_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93549	Sample	BB93549	targeted	Absent	Sample	Sspa_Oph2007_COI	BD60	COI	45	BB93549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD60	GAAGGCCTTCCGATGATC	153.17593
BB93549	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36339	WGS84	BB93549	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93549_Upin_Bot2011_COI	BD60	1	BB93549_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93549	Sample	BB93549	targeted	Absent	Sample	Upin_Bot2011_COI	BD60	COI	45	BB93549	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD60	CTTTAATTACAGCGTTTTTATTGTTGT	153.17593
BB93550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37147	WGS84	BB93550	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93550_Cmae_Bot2011_COI	BD55	1	BB93550_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93550	Sample	BB93550	targeted	Absent	Sample	Cmae_Bot2011_COI	BD55	COI	45	BB93550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD55	ATGAACAGTCTATCCTCCTTTAG	153.1703
BB93550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37147	WGS84	BB93550	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93550_Msal_Bot2012_COI	BD55	1	BB93550_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93550	Sample	BB93550	targeted	Absent	Sample	Msal_Bot2012_COI	BD55	COI	45	BB93550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD55	GYTAGTTCCRATGATGTTAGCTG	153.1703
BB93552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40307	WGS84	BB93552	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93552_Aamu_Bax2006_COI	BD35	1	BB93552_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93552	Sample	BB93552	targeted	Absent	Sample	Aamu_Bax2006_COI	BD35	COI	45	BB93552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD35	GCACAACCGGGATCTTTACTTC	153.15355
BB93552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40307	WGS84	BB93552	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93552_Pcan_Bot2011_IGS	BD35	1	BB93552_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93552	Sample	BB93552	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD35	IGS	45	BB93552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD35	CGTAATCCTCAGTACTGGCTA	153.15355
BB93552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40307	WGS84	BB93552	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93552_Sspa_Oph2007_COI	BD35	1	BB93552_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93552	Sample	BB93552	targeted	Absent	Sample	Sspa_Oph2007_COI	BD35	COI	45	BB93552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD35	GAAGGCCTTCCGATGATC	153.15355
BB93552	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40307	WGS84	BB93552	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93552_Upin_Bot2011_COI	BD35	1	BB93552_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93552	Sample	BB93552	targeted	Absent	Sample	Upin_Bot2011_COI	BD35	COI	45	BB93552	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD35	CTTTAATTACAGCGTTTTTATTGTTGT	153.15355
BB93553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43042	WGS84	BB93553	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93553_Mgig_Bot2012_COI	BD25	1	BB93553_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93553	Sample	BB93553	targeted	Absent	Sample	Mgig_Bot2012_COI	BD25	COI	45	BB93553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD25	TCTTATTCGTTGGAGACTTTATAACCCT	153.12364
BB93554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44768	WGS84	BB93554	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93554_Cmae_Bot2011_COI	BD15	1	BB93554_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93554	Sample	BB93554	targeted	Absent	Sample	Cmae_Bot2011_COI	BD15	COI	45	BB93554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD15	ATGAACAGTCTATCCTCCTTTAG	153.0949
BB93554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44768	WGS84	BB93554	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93554_Msal_Bot2012_COI	BD15	1	BB93554_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93554	Sample	BB93554	targeted	Absent	Sample	Msal_Bot2012_COI	BD15	COI	45	BB93554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD15	GYTAGTTCCRATGATGTTAGCTG	153.0949
BB93556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36005	WGS84	BB93556	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93556_Aamu_Bax2006_COI	BD62	1	BB93556_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93556	Sample	BB93556	targeted	Absent	Sample	Aamu_Bax2006_COI	BD62	COI	45	BB93556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD62	GCACAACCGGGATCTTTACTTC	153.17846
BB93556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36005	WGS84	BB93556	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93556_Pcan_Bot2011_IGS	BD62	1	BB93556_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93556	Sample	BB93556	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD62	IGS	45	BB93556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD62	CGTAATCCTCAGTACTGGCTA	153.17846
BB93556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36005	WGS84	BB93556	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93556_Sspa_Oph2007_COI	BD62	1	BB93556_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93556	Sample	BB93556	targeted	Absent	Sample	Sspa_Oph2007_COI	BD62	COI	45	BB93556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD62	GAAGGCCTTCCGATGATC	153.17846
BB93556	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36005	WGS84	BB93556	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93556_Upin_Bot2011_COI	BD62	1	BB93556_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93556	Sample	BB93556	targeted	Absent	Sample	Upin_Bot2011_COI	BD62	COI	45	BB93556	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD62	CTTTAATTACAGCGTTTTTATTGTTGT	153.17846
BB93557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36529	WGS84	BB93557	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93557_Mgig_Bot2012_COI	BD59	1	BB93557_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93557	Sample	BB93557	targeted	Absent	Sample	Mgig_Bot2012_COI	BD59	COI	45	BB93557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD59	TCTTATTCGTTGGAGACTTTATAACCCT	153.17455
BB93558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36857	WGS84	BB93558	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93558_Cmae_Bot2011_COI	BD57	1	BB93558_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93558	Sample	BB93558	targeted	Absent	Sample	Cmae_Bot2011_COI	BD57	COI	45	BB93558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD57	ATGAACAGTCTATCCTCCTTTAG	153.17257
BB93558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.36857	WGS84	BB93558	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93558_Msal_Bot2012_COI	BD57	1	BB93558_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93558	Sample	BB93558	targeted	Absent	Sample	Msal_Bot2012_COI	BD57	COI	45	BB93558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD57	GYTAGTTCCRATGATGTTAGCTG	153.17257
BB93560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.37475	WGS84	BB93560	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93560_Mgig_Bot2012_COI	BD53	1	BB93560_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93560	Sample	BB93560	targeted	Absent	Sample	Mgig_Bot2012_COI	BD53	COI	45	BB93560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD53	TCTTATTCGTTGGAGACTTTATAACCCT	153.16829
BB93561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3773	WGS84	BB93561	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93561_Cmae_Bot2011_COI	BD51	1	BB93561_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93561	Sample	BB93561	targeted	Absent	Sample	Cmae_Bot2011_COI	BD51	COI	45	BB93561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD51	ATGAACAGTCTATCCTCCTTTAG	153.16576
BB93561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.3773	WGS84	BB93561	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93561_Msal_Bot2012_COI	BD51	1	BB93561_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93561	Sample	BB93561	targeted	Absent	Sample	Msal_Bot2012_COI	BD51	COI	45	BB93561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD51	GYTAGTTCCRATGATGTTAGCTG	153.16576
BB93563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38398	WGS84	BB93563	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93563_Aamu_Bax2006_COI	BD46	1	BB93563_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93563	Sample	BB93563	targeted	Absent	Sample	Aamu_Bax2006_COI	BD46	COI	45	BB93563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD46	GCACAACCGGGATCTTTACTTC	153.16056
BB93563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38398	WGS84	BB93563	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93563_Pcan_Bot2011_IGS	BD46	1	BB93563_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93563	Sample	BB93563	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD46	IGS	45	BB93563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD46	CGTAATCCTCAGTACTGGCTA	153.16056
BB93563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38398	WGS84	BB93563	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93563_Sspa_Oph2007_COI	BD46	1	BB93563_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93563	Sample	BB93563	targeted	Absent	Sample	Sspa_Oph2007_COI	BD46	COI	45	BB93563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD46	GAAGGCCTTCCGATGATC	153.16056
BB93563	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38398	WGS84	BB93563	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93563_Upin_Bot2011_COI	BD46	1	BB93563_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93563	Sample	BB93563	targeted	Absent	Sample	Upin_Bot2011_COI	BD46	COI	45	BB93563	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD46	CTTTAATTACAGCGTTTTTATTGTTGT	153.16056
BB93564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.38628	WGS84	BB93564	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93564_Mgig_Bot2012_COI	BD44	1	BB93564_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93564	Sample	BB93564	targeted	Absent	Sample	Mgig_Bot2012_COI	BD44	COI	45	BB93564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD44	TCTTATTCGTTGGAGACTTTATAACCCT	153.15955
BB93565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.39045	WGS84	BB93565	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93565_Cmae_Bot2011_COI	BD42	1	BB93565_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93565	Sample	BB93565	targeted	Absent	Sample	Cmae_Bot2011_COI	BD42	COI	45	BB93565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD42	ATGAACAGTCTATCCTCCTTTAG	153.15746
BB93565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.39045	WGS84	BB93565	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	21/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93565_Msal_Bot2012_COI	BD42	1	BB93565_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93565	Sample	BB93565	targeted	Absent	Sample	Msal_Bot2012_COI	BD42	COI	45	BB93565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD42	GYTAGTTCCRATGATGTTAGCTG	153.15746
BB93567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40091	WGS84	BB93567	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93567_Aamu_Bax2006_COI	BD39	1	BB93567_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93567	Sample	BB93567	targeted	Absent	Sample	Aamu_Bax2006_COI	BD39	COI	45	BB93567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD39	GCACAACCGGGATCTTTACTTC	153.16222
BB93567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40091	WGS84	BB93567	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93567_Pcan_Bot2011_IGS	BD39	1	BB93567_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93567	Sample	BB93567	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD39	IGS	45	BB93567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD39	CGTAATCCTCAGTACTGGCTA	153.16222
BB93567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40091	WGS84	BB93567	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93567_Sspa_Oph2007_COI	BD39	1	BB93567_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93567	Sample	BB93567	targeted	Absent	Sample	Sspa_Oph2007_COI	BD39	COI	45	BB93567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD39	GAAGGCCTTCCGATGATC	153.16222
BB93567	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40091	WGS84	BB93567	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93567_Upin_Bot2011_COI	BD39	1	BB93567_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93567	Sample	BB93567	targeted	Absent	Sample	Upin_Bot2011_COI	BD39	COI	45	BB93567	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD39	CTTTAATTACAGCGTTTTTATTGTTGT	153.16222
BB93568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40102	WGS84	BB93568	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93568_Mgig_Bot2012_COI	BD37	1	BB93568_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93568	Sample	BB93568	targeted	Absent	Sample	Mgig_Bot2012_COI	BD37	COI	45	BB93568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD37	TCTTATTCGTTGGAGACTTTATAACCCT	153.15883
BB93569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40516	WGS84	BB93569	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93569_Cmae_Bot2011_COI	BD33	1	BB93569_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93569	Sample	BB93569	targeted	Absent	Sample	Cmae_Bot2011_COI	BD33	COI	45	BB93569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD33	ATGAACAGTCTATCCTCCTTTAG	153.15148
BB93569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.40516	WGS84	BB93569	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93569_Msal_Bot2012_COI	BD33	1	BB93569_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93569	Sample	BB93569	targeted	Absent	Sample	Msal_Bot2012_COI	BD33	COI	45	BB93569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD33	GYTAGTTCCRATGATGTTAGCTG	153.15148
BB93570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.4123	WGS84	BB93570	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93570_Aamu_Bax2006_COI	BD32	1	BB93570_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93570	Sample	BB93570	targeted	Absent	Sample	Aamu_Bax2006_COI	BD32	COI	45	BB93570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD32	GCACAACCGGGATCTTTACTTC	153.14395
BB93570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.4123	WGS84	BB93570	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93570_Pcan_Bot2011_IGS	BD32	1	BB93570_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93570	Sample	BB93570	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD32	IGS	45	BB93570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD32	CGTAATCCTCAGTACTGGCTA	153.14395
BB93570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.4123	WGS84	BB93570	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93570_Sspa_Oph2007_COI	BD32	1	BB93570_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93570	Sample	BB93570	targeted	Absent	Sample	Sspa_Oph2007_COI	BD32	COI	45	BB93570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD32	GAAGGCCTTCCGATGATC	153.14395
BB93570	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.4123	WGS84	BB93570	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93570_Upin_Bot2011_COI	BD32	1	BB93570_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93570	Sample	BB93570	targeted	Absent	Sample	Upin_Bot2011_COI	BD32	COI	45	BB93570	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD32	CTTTAATTACAGCGTTTTTATTGTTGT	153.14395
BB93571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.41593	WGS84	BB93571	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93571_Mgig_Bot2012_COI	BD31	1	BB93571_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93571	Sample	BB93571	targeted	Absent	Sample	Mgig_Bot2012_COI	BD31	COI	45	BB93571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD31	TCTTATTCGTTGGAGACTTTATAACCCT	153.14258
BB93572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42178	WGS84	BB93572	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93572_Cmae_Bot2011_COI	BD29	1	BB93572_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93572	Sample	BB93572	targeted	Absent	Sample	Cmae_Bot2011_COI	BD29	COI	45	BB93572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD29	ATGAACAGTCTATCCTCCTTTAG	153.13521
BB93572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42178	WGS84	BB93572	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93572_Msal_Bot2012_COI	BD29	1	BB93572_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93572	Sample	BB93572	targeted	Absent	Sample	Msal_Bot2012_COI	BD29	COI	45	BB93572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD29	GYTAGTTCCRATGATGTTAGCTG	153.13521
BB93574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.428	WGS84	BB93574	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93574_Aamu_Bax2006_COI	BD24	1	BB93574_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93574	Sample	BB93574	targeted	Absent	Sample	Aamu_Bax2006_COI	BD24	COI	45	BB93574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD24	GCACAACCGGGATCTTTACTTC	153.12199
BB93574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.428	WGS84	BB93574	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93574_Pcan_Bot2011_IGS	BD24	1	BB93574_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93574	Sample	BB93574	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD24	IGS	45	BB93574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD24	CGTAATCCTCAGTACTGGCTA	153.12199
BB93574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.428	WGS84	BB93574	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93574_Sspa_Oph2007_COI	BD24	1	BB93574_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93574	Sample	BB93574	targeted	Absent	Sample	Sspa_Oph2007_COI	BD24	COI	45	BB93574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD24	GAAGGCCTTCCGATGATC	153.12199
BB93574	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.428	WGS84	BB93574	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93574_Upin_Bot2011_COI	BD24	1	BB93574_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93574	Sample	BB93574	targeted	Absent	Sample	Upin_Bot2011_COI	BD24	COI	45	BB93574	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD24	CTTTAATTACAGCGTTTTTATTGTTGT	153.12199
BB93575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.42982	WGS84	BB93575	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93575_Mgig_Bot2012_COI	BD21	1	BB93575_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93575	Sample	BB93575	targeted	Absent	Sample	Mgig_Bot2012_COI	BD21	COI	45	BB93575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD21	TCTTATTCGTTGGAGACTTTATAACCCT	153.12339
BB93576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43122	WGS84	BB93576	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93576_Cmae_Bot2011_COI	BD18	1	BB93576_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93576	Sample	BB93576	targeted	Absent	Sample	Cmae_Bot2011_COI	BD18	COI	45	BB93576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD18	ATGAACAGTCTATCCTCCTTTAG	153.11783
BB93576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.43122	WGS84	BB93576	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93576_Msal_Bot2012_COI	BD18	1	BB93576_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93576	Sample	BB93576	targeted	Absent	Sample	Msal_Bot2012_COI	BD18	COI	45	BB93576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD18	GYTAGTTCCRATGATGTTAGCTG	153.11783
BB93578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44561	WGS84	BB93578	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93578_Aamu_Bax2006_COI	BD14	1	BB93578_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93578	Sample	BB93578	targeted	Absent	Sample	Aamu_Bax2006_COI	BD14	COI	45	BB93578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD14	GCACAACCGGGATCTTTACTTC	153.08298
BB93578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44561	WGS84	BB93578	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93578_Pcan_Bot2011_IGS	BD14	1	BB93578_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93578	Sample	BB93578	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD14	IGS	45	BB93578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD14	CGTAATCCTCAGTACTGGCTA	153.08298
BB93578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44561	WGS84	BB93578	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93578_Sspa_Oph2007_COI	BD14	1	BB93578_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93578	Sample	BB93578	targeted	Absent	Sample	Sspa_Oph2007_COI	BD14	COI	45	BB93578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD14	GAAGGCCTTCCGATGATC	153.08298
BB93578	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44561	WGS84	BB93578	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93578_Upin_Bot2011_COI	BD14	1	BB93578_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93578	Sample	BB93578	targeted	Absent	Sample	Upin_Bot2011_COI	BD14	COI	45	BB93578	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD14	CTTTAATTACAGCGTTTTTATTGTTGT	153.08298
BB93579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44615	WGS84	BB93579	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93579_Mgig_Bot2012_COI	BD9	1	BB93579_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93579	Sample	BB93579	targeted	Absent	Sample	Mgig_Bot2012_COI	BD9	COI	45	BB93579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD9	TCTTATTCGTTGGAGACTTTATAACCCT	153.07929
BB93581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44533	WGS84	BB93581	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93581_Aamu_Bax2006_COI	BD7	1	BB93581_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93581	Sample	BB93581	targeted	Absent	Sample	Aamu_Bax2006_COI	BD7	COI	45	BB93581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD7	GCACAACCGGGATCTTTACTTC	153.07768
BB93581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44533	WGS84	BB93581	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93581_Pcan_Bot2011_IGS	BD7	1	BB93581_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93581	Sample	BB93581	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD7	IGS	45	BB93581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD7	CGTAATCCTCAGTACTGGCTA	153.07768
BB93581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44533	WGS84	BB93581	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93581_Sspa_Oph2007_COI	BD7	1	BB93581_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93581	Sample	BB93581	targeted	Absent	Sample	Sspa_Oph2007_COI	BD7	COI	45	BB93581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD7	GAAGGCCTTCCGATGATC	153.07768
BB93581	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44533	WGS84	BB93581	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93581_Upin_Bot2011_COI	BD7	1	BB93581_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93581	Sample	BB93581	targeted	Absent	Sample	Upin_Bot2011_COI	BD7	COI	45	BB93581	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD7	CTTTAATTACAGCGTTTTTATTGTTGT	153.07768
BB93582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44446	WGS84	BB93582	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93582_Mgig_Bot2012_COI	BD6	1	BB93582_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93582	Sample	BB93582	targeted	Absent	Sample	Mgig_Bot2012_COI	BD6	COI	45	BB93582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD6	TCTTATTCGTTGGAGACTTTATAACCCT	153.07696
BB93583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44569	WGS84	BB93583	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93583_Cmae_Bot2011_COI	BD4	1	BB93583_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93583	Sample	BB93583	targeted	Absent	Sample	Cmae_Bot2011_COI	BD4	COI	45	BB93583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD4	ATGAACAGTCTATCCTCCTTTAG	153.07546
BB93583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44569	WGS84	BB93583	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93583_Msal_Bot2012_COI	BD4	1	BB93583_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93583	Sample	BB93583	targeted	Absent	Sample	Msal_Bot2012_COI	BD4	COI	45	BB93583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD4	GYTAGTTCCRATGATGTTAGCTG	153.07546
BB93585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44293	WGS84	BB93585	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93585_Aamu_Bax2006_COI	BD2	1	BB93585_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93585	Sample	BB93585	targeted	Absent	Sample	Aamu_Bax2006_COI	BD2	COI	45	BB93585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD2	GCACAACCGGGATCTTTACTTC	153.07323
BB93585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44293	WGS84	BB93585	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93585_Pcan_Bot2011_IGS	BD2	1	BB93585_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93585	Sample	BB93585	targeted	Absent	Sample	Pcan_Bot2011_IGS	BD2	IGS	45	BB93585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD2	CGTAATCCTCAGTACTGGCTA	153.07323
BB93585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44293	WGS84	BB93585	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93585_Sspa_Oph2007_COI	BD2	1	BB93585_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93585	Sample	BB93585	targeted	Absent	Sample	Sspa_Oph2007_COI	BD2	COI	45	BB93585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD2	GAAGGCCTTCCGATGATC	153.07323
BB93585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-27.44293	WGS84	BB93585	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Autumn	20/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93585_Upin_Bot2011_COI	BD2	1	BB93585_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93585	Sample	BB93585	targeted	Absent	Sample	Upin_Bot2011_COI	BD2	COI	45	BB93585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BD2	CTTTAATTACAGCGTTTTTATTGTTGT	153.07323
BB93586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24295	WGS84	BB93586	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93586_Mgig_Bot2012_COI	YD140	1	BB93586_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93586	Sample	BB93586	targeted	Absent	Sample	Mgig_Bot2012_COI	YD140	COI	45	BB93586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD140	TCTTATTCGTTGGAGACTTTATAACCCT	149.30158
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93587_Cmae_Bot2011_COI	YD138	1	BB93587_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Cmae_Bot2011_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	ATGAACAGTCTATCCTCCTTTAG	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93587_Hsan_Wil2021_COI	YD138	1	BB93587_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Hsan_Wil2021_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	CCTGGGCCGGTATAGTAGGT	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93587_Mros_Gib2022_COI	YD138	1	BB93587_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Mros_Gib2022_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93587_Msal_Bot2012_COI	YD138	1	BB93587_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Msal_Bot2012_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	GYTAGTTCCRATGATGTTAGCTG	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93587_Pvir_Dia2013_COI	YD138	1	BB93587_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Pvir_Dia2013_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	CTTAGTGGCATTAATTCGDAATCC	149.31354
BB93587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25865	WGS84	BB93587	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93587_Rhar_Sim2018_COI	YD138	1	BB93587_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93587	Sample	BB93587	targeted	Absent	Sample	Rhar_Sim2018_COI	YD138	COI	45	BB93587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD138	CTGCTGCTATTGCTCATG	149.31354
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93589_Aamu_Bax2006_COI	YD134	1	BB93589_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Aamu_Bax2006_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	GCACAACCGGGATCTTTACTTC	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93589_Pcan_Bot2011_IGS	YD134	1	BB93589_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD134	IGS	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	CGTAATCCTCAGTACTGGCTA	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93589_Pper_Dia2013_COI	YD134	1	BB93589_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Pper_Dia2013_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	CTTAGTGGCATTAATTCGDAATCC	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93589_Sspa_Oph2007_COI	YD134	1	BB93589_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Sspa_Oph2007_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	GAAGGCCTTCCGATGATC	149.31226
BB93589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25958	WGS84	BB93589	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93589_Upin_Bot2011_COI	YD134	1	BB93589_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93589	Sample	BB93589	targeted	Absent	Sample	Upin_Bot2011_COI	YD134	COI	45	BB93589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD134	CTTTAATTACAGCGTTTTTATTGTTGT	149.31226
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93590_Cmae_Bot2011_COI	YD132	1	BB93590_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Cmae_Bot2011_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	ATGAACAGTCTATCCTCCTTTAG	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93590_Hsan_Wil2021_COI	YD132	1	BB93590_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Hsan_Wil2021_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	CCTGGGCCGGTATAGTAGGT	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93590_Mros_Gib2022_COI	YD132	1	BB93590_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Mros_Gib2022_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93590_Msal_Bot2012_COI	YD132	1	BB93590_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Msal_Bot2012_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	GYTAGTTCCRATGATGTTAGCTG	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93590_Pvir_Dia2013_COI	YD132	1	BB93590_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Pvir_Dia2013_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	CTTAGTGGCATTAATTCGDAATCC	149.31331
BB93590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26128	WGS84	BB93590	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93590_Rhar_Sim2018_COI	YD132	1	BB93590_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93590	Sample	BB93590	targeted	Absent	Sample	Rhar_Sim2018_COI	YD132	COI	45	BB93590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD132	CTGCTGCTATTGCTCATG	149.31331
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93592_Aamu_Bax2006_COI	YD129	1	BB93592_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Aamu_Bax2006_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	GCACAACCGGGATCTTTACTTC	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93592_Pcan_Bot2011_IGS	YD129	1	BB93592_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD129	IGS	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	CGTAATCCTCAGTACTGGCTA	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93592_Pper_Dia2013_COI	YD129	1	BB93592_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Pper_Dia2013_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	CTTAGTGGCATTAATTCGDAATCC	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93592_Sspa_Oph2007_COI	YD129	1	BB93592_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Sspa_Oph2007_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	GAAGGCCTTCCGATGATC	149.31412
BB93592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26274	WGS84	BB93592	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93592_Upin_Bot2011_COI	YD129	1	BB93592_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93592	Sample	BB93592	targeted	Absent	Sample	Upin_Bot2011_COI	YD129	COI	45	BB93592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD129	CTTTAATTACAGCGTTTTTATTGTTGT	149.31412
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93593_Cjap_Sim2018_COI	YD128	1	BB93593_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Cjap_Sim2018_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93593_Esin_And2018_Cyb	YD128	1	BB93593_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Esin_And2018_Cyb	YD128	Cytb	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	ACCCCTCCTCATATCCAACCA	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93593_Mgig_Bot2012_COI	YD128	1	BB93593_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Mgig_Bot2012_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	TCTTATTCGTTGGAGACTTTATAACCCT	149.31464
BB93593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26344	WGS84	BB93593	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93593_Mjap_Gib2022_COI	YD128	1	BB93593_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93593	Sample	BB93593	targeted	Absent	Sample	Mjap_Gib2022_COI	YD128	COI	45	BB93593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD128	AATTTAATTGTGACTAGGCATGGTCTG	149.31464
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93594_Cmae_Bot2011_COI	YD127	1	BB93594_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Cmae_Bot2011_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	ATGAACAGTCTATCCTCCTTTAG	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93594_Hsan_Wil2021_COI	YD127	1	BB93594_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Hsan_Wil2021_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	CCTGGGCCGGTATAGTAGGT	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93594_Mros_Gib2022_COI	YD127	1	BB93594_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Mros_Gib2022_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93594_Msal_Bot2012_COI	YD127	1	BB93594_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Msal_Bot2012_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	GYTAGTTCCRATGATGTTAGCTG	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93594_Pvir_Dia2013_COI	YD127	1	BB93594_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Pvir_Dia2013_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	CTTAGTGGCATTAATTCGDAATCC	149.31381
BB93594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26355	WGS84	BB93594	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93594_Rhar_Sim2018_COI	YD127	1	BB93594_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93594	Sample	BB93594	targeted	Absent	Sample	Rhar_Sim2018_COI	YD127	COI	45	BB93594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD127	CTGCTGCTATTGCTCATG	149.31381
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93596_Aamu_Bax2006_COI	YD123	1	BB93596_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Aamu_Bax2006_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	GCACAACCGGGATCTTTACTTC	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93596_Pcan_Bot2011_IGS	YD123	1	BB93596_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD123	IGS	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	CGTAATCCTCAGTACTGGCTA	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93596_Pper_Dia2013_COI	YD123	1	BB93596_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Pper_Dia2013_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	CTTAGTGGCATTAATTCGDAATCC	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93596_Sspa_Oph2007_COI	YD123	1	BB93596_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Sspa_Oph2007_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	GAAGGCCTTCCGATGATC	149.31475
BB93596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26474	WGS84	BB93596	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93596_Upin_Bot2011_COI	YD123	1	BB93596_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93596	Sample	BB93596	targeted	Absent	Sample	Upin_Bot2011_COI	YD123	COI	45	BB93596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD123	CTTTAATTACAGCGTTTTTATTGTTGT	149.31475
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93597_Cjap_Sim2018_COI	YD122	1	BB93597_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Cjap_Sim2018_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	TTAATATACGGTCATTTGGTATGAGTATAGATC	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93597_Esin_And2018_Cyb	YD122	1	BB93597_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Esin_And2018_Cyb	YD122	Cytb	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	ACCCCTCCTCATATCCAACCA	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93597_Mgig_Bot2012_COI	YD122	1	BB93597_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Mgig_Bot2012_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	TCTTATTCGTTGGAGACTTTATAACCCT	149.31396
BB93597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26494	WGS84	BB93597	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93597_Mjap_Gib2022_COI	YD122	1	BB93597_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93597	Sample	BB93597	targeted	Absent	Sample	Mjap_Gib2022_COI	YD122	COI	45	BB93597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD122	AATTTAATTGTGACTAGGCATGGTCTG	149.31396
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93598_Cmae_Bot2011_COI	YD118	1	BB93598_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Cmae_Bot2011_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	ATGAACAGTCTATCCTCCTTTAG	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93598_Hsan_Wil2021_COI	YD118	1	BB93598_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Hsan_Wil2021_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	CCTGGGCCGGTATAGTAGGT	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93598_Mros_Gib2022_COI	YD118	1	BB93598_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Mros_Gib2022_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	ACTTCTACCTCCTGCTCTTCTACTTCTC	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93598_Msal_Bot2012_COI	YD118	1	BB93598_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Msal_Bot2012_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	GYTAGTTCCRATGATGTTAGCTG	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93598_Pvir_Dia2013_COI	YD118	1	BB93598_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Pvir_Dia2013_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	CTTAGTGGCATTAATTCGDAATCC	149.31427
BB93598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26484	WGS84	BB93598	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93598_Rhar_Sim2018_COI	YD118	1	BB93598_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93598	Sample	BB93598	targeted	Absent	Sample	Rhar_Sim2018_COI	YD118	COI	45	BB93598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD118	CTGCTGCTATTGCTCATG	149.31427
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93600_Aamu_Bax2006_COI	YD116	1	BB93600_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Aamu_Bax2006_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	GCACAACCGGGATCTTTACTTC	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93600_Pcan_Bot2011_IGS	YD116	1	BB93600_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD116	IGS	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	CGTAATCCTCAGTACTGGCTA	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93600_Pper_Dia2013_COI	YD116	1	BB93600_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Pper_Dia2013_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	CTTAGTGGCATTAATTCGDAATCC	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93600_Sspa_Oph2007_COI	YD116	1	BB93600_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Sspa_Oph2007_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	GAAGGCCTTCCGATGATC	149.31627
BB93600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26566	WGS84	BB93600	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93600_Upin_Bot2011_COI	YD116	1	BB93600_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93600	Sample	BB93600	targeted	Absent	Sample	Upin_Bot2011_COI	YD116	COI	45	BB93600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD116	CTTTAATTACAGCGTTTTTATTGTTGT	149.31627
BB93601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26553	WGS84	BB93601	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93601_Mgig_Bot2012_COI	YD114	1	BB93601_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93601	Sample	BB93601	targeted	Absent	Sample	Mgig_Bot2012_COI	YD114	COI	45	BB93601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD114	TCTTATTCGTTGGAGACTTTATAACCCT	149.31434
BB93602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26621	WGS84	BB93602	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93602_Cmae_Bot2011_COI	YD113	1	BB93602_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93602	Sample	BB93602	targeted	Absent	Sample	Cmae_Bot2011_COI	YD113	COI	45	BB93602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD113	ATGAACAGTCTATCCTCCTTTAG	149.31567
BB93602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26621	WGS84	BB93602	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93602_Msal_Bot2012_COI	YD113	1	BB93602_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93602	Sample	BB93602	targeted	Absent	Sample	Msal_Bot2012_COI	YD113	COI	45	BB93602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD113	GYTAGTTCCRATGATGTTAGCTG	149.31567
BB93604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25593	WGS84	BB93604	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93604_Aamu_Bax2006_COI	YD108	1	BB93604_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93604	Sample	BB93604	targeted	Absent	Sample	Aamu_Bax2006_COI	YD108	COI	45	BB93604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD108	GCACAACCGGGATCTTTACTTC	149.30946
BB93604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25593	WGS84	BB93604	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93604_Pcan_Bot2011_IGS	YD108	1	BB93604_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93604	Sample	BB93604	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD108	IGS	45	BB93604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD108	CGTAATCCTCAGTACTGGCTA	149.30946
BB93604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25593	WGS84	BB93604	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93604_Sspa_Oph2007_COI	YD108	1	BB93604_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93604	Sample	BB93604	targeted	Absent	Sample	Sspa_Oph2007_COI	YD108	COI	45	BB93604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD108	GAAGGCCTTCCGATGATC	149.30946
BB93604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25593	WGS84	BB93604	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93604_Upin_Bot2011_COI	YD108	1	BB93604_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93604	Sample	BB93604	targeted	Absent	Sample	Upin_Bot2011_COI	YD108	COI	45	BB93604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD108	CTTTAATTACAGCGTTTTTATTGTTGT	149.30946
BB93605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26828	WGS84	BB93605	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93605_Mgig_Bot2012_COI	YD107	1	BB93605_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93605	Sample	BB93605	targeted	Absent	Sample	Mgig_Bot2012_COI	YD107	COI	45	BB93605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD107	TCTTATTCGTTGGAGACTTTATAACCCT	149.31404
BB93606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26754	WGS84	BB93606	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93606_Cmae_Bot2011_COI	YD106	1	BB93606_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93606	Sample	BB93606	targeted	Absent	Sample	Cmae_Bot2011_COI	YD106	COI	45	BB93606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD106	ATGAACAGTCTATCCTCCTTTAG	149.31291
BB93606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26754	WGS84	BB93606	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93606_Msal_Bot2012_COI	YD106	1	BB93606_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93606	Sample	BB93606	targeted	Absent	Sample	Msal_Bot2012_COI	YD106	COI	45	BB93606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD106	GYTAGTTCCRATGATGTTAGCTG	149.31291
BB93608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25174	WGS84	BB93608	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93608_Aamu_Bax2006_COI	YD103	1	BB93608_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93608	Sample	BB93608	targeted	Absent	Sample	Aamu_Bax2006_COI	YD103	COI	45	BB93608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD103	GCACAACCGGGATCTTTACTTC	149.30413
BB93608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25174	WGS84	BB93608	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93608_Pcan_Bot2011_IGS	YD103	1	BB93608_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93608	Sample	BB93608	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD103	IGS	45	BB93608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD103	CGTAATCCTCAGTACTGGCTA	149.30413
BB93608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25174	WGS84	BB93608	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93608_Sspa_Oph2007_COI	YD103	1	BB93608_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93608	Sample	BB93608	targeted	Absent	Sample	Sspa_Oph2007_COI	YD103	COI	45	BB93608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD103	GAAGGCCTTCCGATGATC	149.30413
BB93608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25174	WGS84	BB93608	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93608_Upin_Bot2011_COI	YD103	1	BB93608_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93608	Sample	BB93608	targeted	Absent	Sample	Upin_Bot2011_COI	YD103	COI	45	BB93608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD103	CTTTAATTACAGCGTTTTTATTGTTGT	149.30413
BB93609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25226	WGS84	BB93609	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93609_Mgig_Bot2012_COI	YD101	1	BB93609_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93609	Sample	BB93609	targeted	Absent	Sample	Mgig_Bot2012_COI	YD101	COI	45	BB93609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD101	TCTTATTCGTTGGAGACTTTATAACCCT	149.30554
BB93611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25356	WGS84	BB93611	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93611_Aamu_Bax2006_COI	YD98	1	BB93611_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93611	Sample	BB93611	targeted	Absent	Sample	Aamu_Bax2006_COI	YD98	COI	45	BB93611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD98	GCACAACCGGGATCTTTACTTC	149.30649
BB93611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25356	WGS84	BB93611	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93611_Pcan_Bot2011_IGS	YD98	1	BB93611_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93611	Sample	BB93611	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD98	IGS	45	BB93611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD98	CGTAATCCTCAGTACTGGCTA	149.30649
BB93611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25356	WGS84	BB93611	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93611_Sspa_Oph2007_COI	YD98	1	BB93611_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93611	Sample	BB93611	targeted	Absent	Sample	Sspa_Oph2007_COI	YD98	COI	45	BB93611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD98	GAAGGCCTTCCGATGATC	149.30649
BB93611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25356	WGS84	BB93611	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93611_Upin_Bot2011_COI	YD98	1	BB93611_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93611	Sample	BB93611	targeted	Absent	Sample	Upin_Bot2011_COI	YD98	COI	45	BB93611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD98	CTTTAATTACAGCGTTTTTATTGTTGT	149.30649
BB93612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25435	WGS84	BB93612	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93612_Mgig_Bot2012_COI	YD97	1	BB93612_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93612	Sample	BB93612	targeted	Absent	Sample	Mgig_Bot2012_COI	YD97	COI	45	BB93612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD97	TCTTATTCGTTGGAGACTTTATAACCCT	149.30611
BB93613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25424	WGS84	BB93613	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93613_Cmae_Bot2011_COI	YD96	1	BB93613_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93613	Sample	BB93613	targeted	Absent	Sample	Cmae_Bot2011_COI	YD96	COI	45	BB93613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD96	ATGAACAGTCTATCCTCCTTTAG	149.30705
BB93613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25424	WGS84	BB93613	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93613_Msal_Bot2012_COI	YD96	1	BB93613_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93613	Sample	BB93613	targeted	Absent	Sample	Msal_Bot2012_COI	YD96	COI	45	BB93613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD96	GYTAGTTCCRATGATGTTAGCTG	149.30705
BB93615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25704	WGS84	BB93615	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93615_Aamu_Bax2006_COI	YD92	1	BB93615_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93615	Sample	BB93615	targeted	Absent	Sample	Aamu_Bax2006_COI	YD92	COI	45	BB93615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD92	GCACAACCGGGATCTTTACTTC	149.30826
BB93615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25704	WGS84	BB93615	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93615_Pcan_Bot2011_IGS	YD92	1	BB93615_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93615	Sample	BB93615	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD92	IGS	45	BB93615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD92	CGTAATCCTCAGTACTGGCTA	149.30826
BB93615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25704	WGS84	BB93615	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93615_Sspa_Oph2007_COI	YD92	1	BB93615_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93615	Sample	BB93615	targeted	Absent	Sample	Sspa_Oph2007_COI	YD92	COI	45	BB93615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD92	GAAGGCCTTCCGATGATC	149.30826
BB93615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25704	WGS84	BB93615	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93615_Upin_Bot2011_COI	YD92	1	BB93615_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93615	Sample	BB93615	targeted	Absent	Sample	Upin_Bot2011_COI	YD92	COI	45	BB93615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD92	CTTTAATTACAGCGTTTTTATTGTTGT	149.30826
BB93616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25693	WGS84	BB93616	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93616_Mgig_Bot2012_COI	YD91	1	BB93616_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93616	Sample	BB93616	targeted	Absent	Sample	Mgig_Bot2012_COI	YD91	COI	45	BB93616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD91	TCTTATTCGTTGGAGACTTTATAACCCT	149.30923
BB93617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25837	WGS84	BB93617	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93617_Cmae_Bot2011_COI	YD89	1	BB93617_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93617	Sample	BB93617	targeted	Absent	Sample	Cmae_Bot2011_COI	YD89	COI	45	BB93617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD89	ATGAACAGTCTATCCTCCTTTAG	149.30937
BB93617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25837	WGS84	BB93617	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93617_Msal_Bot2012_COI	YD89	1	BB93617_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93617	Sample	BB93617	targeted	Absent	Sample	Msal_Bot2012_COI	YD89	COI	45	BB93617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD89	GYTAGTTCCRATGATGTTAGCTG	149.30937
BB93619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26054	WGS84	BB93619	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93619_Aamu_Bax2006_COI	YD85	1	BB93619_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93619	Sample	BB93619	targeted	Absent	Sample	Aamu_Bax2006_COI	YD85	COI	45	BB93619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD85	GCACAACCGGGATCTTTACTTC	149.31013
BB93619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26054	WGS84	BB93619	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93619_Pcan_Bot2011_IGS	YD85	1	BB93619_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93619	Sample	BB93619	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD85	IGS	45	BB93619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD85	CGTAATCCTCAGTACTGGCTA	149.31013
BB93619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26054	WGS84	BB93619	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93619_Sspa_Oph2007_COI	YD85	1	BB93619_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93619	Sample	BB93619	targeted	Absent	Sample	Sspa_Oph2007_COI	YD85	COI	45	BB93619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD85	GAAGGCCTTCCGATGATC	149.31013
BB93619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26054	WGS84	BB93619	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93619_Upin_Bot2011_COI	YD85	1	BB93619_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93619	Sample	BB93619	targeted	Absent	Sample	Upin_Bot2011_COI	YD85	COI	45	BB93619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD85	CTTTAATTACAGCGTTTTTATTGTTGT	149.31013
BB93620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25977	WGS84	BB93620	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93620_Cmae_Bot2011_COI	YD84	1	BB93620_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93620	Sample	BB93620	targeted	Absent	Sample	Cmae_Bot2011_COI	YD84	COI	45	BB93620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD84	ATGAACAGTCTATCCTCCTTTAG	149.31052
BB93620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25977	WGS84	BB93620	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93620_Msal_Bot2012_COI	YD84	1	BB93620_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93620	Sample	BB93620	targeted	Absent	Sample	Msal_Bot2012_COI	YD84	COI	45	BB93620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD84	GYTAGTTCCRATGATGTTAGCTG	149.31052
BB93622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26411	WGS84	BB93622	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93622_Aamu_Bax2006_COI	YD81	1	BB93622_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93622	Sample	BB93622	targeted	Absent	Sample	Aamu_Bax2006_COI	YD81	COI	45	BB93622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD81	GCACAACCGGGATCTTTACTTC	149.31103
BB93622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26411	WGS84	BB93622	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93622_Pcan_Bot2011_IGS	YD81	1	BB93622_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93622	Sample	BB93622	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD81	IGS	45	BB93622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD81	CGTAATCCTCAGTACTGGCTA	149.31103
BB93622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26411	WGS84	BB93622	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93622_Sspa_Oph2007_COI	YD81	1	BB93622_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93622	Sample	BB93622	targeted	Absent	Sample	Sspa_Oph2007_COI	YD81	COI	45	BB93622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD81	GAAGGCCTTCCGATGATC	149.31103
BB93622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26411	WGS84	BB93622	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93622_Upin_Bot2011_COI	YD81	1	BB93622_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93622	Sample	BB93622	targeted	Absent	Sample	Upin_Bot2011_COI	YD81	COI	45	BB93622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD81	CTTTAATTACAGCGTTTTTATTGTTGT	149.31103
BB93623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26646	WGS84	BB93623	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93623_Mgig_Bot2012_COI	YD76	1	BB93623_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93623	Sample	BB93623	targeted	Absent	Sample	Mgig_Bot2012_COI	YD76	COI	45	BB93623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD76	TCTTATTCGTTGGAGACTTTATAACCCT	149.3169
BB93624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26709	WGS84	BB93624	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93624_Cmae_Bot2011_COI	YD75	1	BB93624_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93624	Sample	BB93624	targeted	Absent	Sample	Cmae_Bot2011_COI	YD75	COI	45	BB93624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD75	ATGAACAGTCTATCCTCCTTTAG	149.31643
BB93624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.26709	WGS84	BB93624	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93624_Msal_Bot2012_COI	YD75	1	BB93624_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93624	Sample	BB93624	targeted	Absent	Sample	Msal_Bot2012_COI	YD75	COI	45	BB93624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD75	GYTAGTTCCRATGATGTTAGCTG	149.31643
BB93626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2573	WGS84	BB93626	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93626_Aamu_Bax2006_COI	YD73	1	BB93626_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93626	Sample	BB93626	targeted	Absent	Sample	Aamu_Bax2006_COI	YD73	COI	45	BB93626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD73	GCACAACCGGGATCTTTACTTC	149.31246
BB93626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2573	WGS84	BB93626	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93626_Pcan_Bot2011_IGS	YD73	1	BB93626_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93626	Sample	BB93626	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD73	IGS	45	BB93626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD73	CGTAATCCTCAGTACTGGCTA	149.31246
BB93626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2573	WGS84	BB93626	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93626_Sspa_Oph2007_COI	YD73	1	BB93626_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93626	Sample	BB93626	targeted	Absent	Sample	Sspa_Oph2007_COI	YD73	COI	45	BB93626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD73	GAAGGCCTTCCGATGATC	149.31246
BB93626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2573	WGS84	BB93626	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93626_Upin_Bot2011_COI	YD73	1	BB93626_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93626	Sample	BB93626	targeted	Absent	Sample	Upin_Bot2011_COI	YD73	COI	45	BB93626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD73	CTTTAATTACAGCGTTTTTATTGTTGT	149.31246
BB93627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25588	WGS84	BB93627	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93627_Mgig_Bot2012_COI	YD71	1	BB93627_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93627	Sample	BB93627	targeted	Absent	Sample	Mgig_Bot2012_COI	YD71	COI	45	BB93627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD71	TCTTATTCGTTGGAGACTTTATAACCCT	149.31227
BB93628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25665	WGS84	BB93628	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93628_Cmae_Bot2011_COI	YD70	1	BB93628_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93628	Sample	BB93628	targeted	Absent	Sample	Cmae_Bot2011_COI	YD70	COI	45	BB93628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD70	ATGAACAGTCTATCCTCCTTTAG	149.31193
BB93628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25665	WGS84	BB93628	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93628_Msal_Bot2012_COI	YD70	1	BB93628_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93628	Sample	BB93628	targeted	Absent	Sample	Msal_Bot2012_COI	YD70	COI	45	BB93628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD70	GYTAGTTCCRATGATGTTAGCTG	149.31193
BB93630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2553	WGS84	BB93630	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93630_Mgig_Bot2012_COI	YD65	1	BB93630_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93630	Sample	BB93630	targeted	Absent	Sample	Mgig_Bot2012_COI	YD65	COI	45	BB93630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD65	TCTTATTCGTTGGAGACTTTATAACCCT	149.31084
BB93631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25608	WGS84	BB93631	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93631_Cmae_Bot2011_COI	YD64	1	BB93631_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93631	Sample	BB93631	targeted	Absent	Sample	Cmae_Bot2011_COI	YD64	COI	45	BB93631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD64	ATGAACAGTCTATCCTCCTTTAG	149.31047
BB93631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25608	WGS84	BB93631	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93631_Msal_Bot2012_COI	YD64	1	BB93631_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93631	Sample	BB93631	targeted	Absent	Sample	Msal_Bot2012_COI	YD64	COI	45	BB93631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD64	GYTAGTTCCRATGATGTTAGCTG	149.31047
BB93633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25462	WGS84	BB93633	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93633_Aamu_Bax2006_COI	YD62	1	BB93633_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93633	Sample	BB93633	targeted	Absent	Sample	Aamu_Bax2006_COI	YD62	COI	45	BB93633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD62	GCACAACCGGGATCTTTACTTC	149.3103
BB93633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25462	WGS84	BB93633	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93633_Pcan_Bot2011_IGS	YD62	1	BB93633_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93633	Sample	BB93633	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD62	IGS	45	BB93633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD62	CGTAATCCTCAGTACTGGCTA	149.3103
BB93633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25462	WGS84	BB93633	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93633_Sspa_Oph2007_COI	YD62	1	BB93633_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93633	Sample	BB93633	targeted	Absent	Sample	Sspa_Oph2007_COI	YD62	COI	45	BB93633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD62	GAAGGCCTTCCGATGATC	149.3103
BB93633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25462	WGS84	BB93633	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93633_Upin_Bot2011_COI	YD62	1	BB93633_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93633	Sample	BB93633	targeted	Absent	Sample	Upin_Bot2011_COI	YD62	COI	45	BB93633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD62	CTTTAATTACAGCGTTTTTATTGTTGT	149.3103
BB93634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25389	WGS84	BB93634	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93634_Mgig_Bot2012_COI	YD59	1	BB93634_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93634	Sample	BB93634	targeted	Absent	Sample	Mgig_Bot2012_COI	YD59	COI	45	BB93634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD59	TCTTATTCGTTGGAGACTTTATAACCCT	149.31064
BB93635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25324	WGS84	BB93635	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93635_Cmae_Bot2011_COI	YD55	1	BB93635_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93635	Sample	BB93635	targeted	Absent	Sample	Cmae_Bot2011_COI	YD55	COI	45	BB93635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD55	ATGAACAGTCTATCCTCCTTTAG	149.30899
BB93635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25324	WGS84	BB93635	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93635_Msal_Bot2012_COI	YD55	1	BB93635_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93635	Sample	BB93635	targeted	Absent	Sample	Msal_Bot2012_COI	YD55	COI	45	BB93635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD55	GYTAGTTCCRATGATGTTAGCTG	149.30899
BB93637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25107	WGS84	BB93637	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93637_Aamu_Bax2006_COI	YD52	1	BB93637_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93637	Sample	BB93637	targeted	Absent	Sample	Aamu_Bax2006_COI	YD52	COI	45	BB93637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD52	GCACAACCGGGATCTTTACTTC	149.30902
BB93637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25107	WGS84	BB93637	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93637_Pcan_Bot2011_IGS	YD52	1	BB93637_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93637	Sample	BB93637	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD52	IGS	45	BB93637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD52	CGTAATCCTCAGTACTGGCTA	149.30902
BB93637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25107	WGS84	BB93637	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93637_Sspa_Oph2007_COI	YD52	1	BB93637_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93637	Sample	BB93637	targeted	Absent	Sample	Sspa_Oph2007_COI	YD52	COI	45	BB93637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD52	GAAGGCCTTCCGATGATC	149.30902
BB93637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25107	WGS84	BB93637	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93637_Upin_Bot2011_COI	YD52	1	BB93637_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93637	Sample	BB93637	targeted	Absent	Sample	Upin_Bot2011_COI	YD52	COI	45	BB93637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD52	CTTTAATTACAGCGTTTTTATTGTTGT	149.30902
BB93638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25162	WGS84	BB93638	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93638_Mgig_Bot2012_COI	YD51	1	BB93638_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93638	Sample	BB93638	targeted	Absent	Sample	Mgig_Bot2012_COI	YD51	COI	45	BB93638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD51	TCTTATTCGTTGGAGACTTTATAACCCT	149.3087
BB93639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24849	WGS84	BB93639	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93639_Cmae_Bot2011_COI	YD47	1	BB93639_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93639	Sample	BB93639	targeted	Absent	Sample	Cmae_Bot2011_COI	YD47	COI	45	BB93639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD47	ATGAACAGTCTATCCTCCTTTAG	149.30523
BB93639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24849	WGS84	BB93639	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93639_Msal_Bot2012_COI	YD47	1	BB93639_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93639	Sample	BB93639	targeted	Absent	Sample	Msal_Bot2012_COI	YD47	COI	45	BB93639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD47	GYTAGTTCCRATGATGTTAGCTG	149.30523
BB93640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24547	WGS84	BB93640	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93640_Aamu_Bax2006_COI	YD46	1	BB93640_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93640	Sample	BB93640	targeted	Absent	Sample	Aamu_Bax2006_COI	YD46	COI	45	BB93640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD46	GCACAACCGGGATCTTTACTTC	149.30575
BB93640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24547	WGS84	BB93640	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93640_Pcan_Bot2011_IGS	YD46	1	BB93640_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93640	Sample	BB93640	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD46	IGS	45	BB93640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD46	CGTAATCCTCAGTACTGGCTA	149.30575
BB93640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24547	WGS84	BB93640	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93640_Sspa_Oph2007_COI	YD46	1	BB93640_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93640	Sample	BB93640	targeted	Absent	Sample	Sspa_Oph2007_COI	YD46	COI	45	BB93640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD46	GAAGGCCTTCCGATGATC	149.30575
BB93640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24547	WGS84	BB93640	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93640_Upin_Bot2011_COI	YD46	1	BB93640_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93640	Sample	BB93640	targeted	Absent	Sample	Upin_Bot2011_COI	YD46	COI	45	BB93640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD46	CTTTAATTACAGCGTTTTTATTGTTGT	149.30575
BB93641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24705	WGS84	BB93641	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93641_Mgig_Bot2012_COI	YD44	1	BB93641_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93641	Sample	BB93641	targeted	Absent	Sample	Mgig_Bot2012_COI	YD44	COI	45	BB93641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD44	TCTTATTCGTTGGAGACTTTATAACCCT	149.30487
BB93642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2454	WGS84	BB93642	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93642_Cmae_Bot2011_COI	YD42	1	BB93642_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93642	Sample	BB93642	targeted	Absent	Sample	Cmae_Bot2011_COI	YD42	COI	45	BB93642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD42	ATGAACAGTCTATCCTCCTTTAG	149.30471
BB93642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2454	WGS84	BB93642	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93642_Msal_Bot2012_COI	YD42	1	BB93642_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93642	Sample	BB93642	targeted	Absent	Sample	Msal_Bot2012_COI	YD42	COI	45	BB93642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD42	GYTAGTTCCRATGATGTTAGCTG	149.30471
BB93644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24415	WGS84	BB93644	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93644_Aamu_Bax2006_COI	YD40	1	BB93644_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93644	Sample	BB93644	targeted	Absent	Sample	Aamu_Bax2006_COI	YD40	COI	45	BB93644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD40	GCACAACCGGGATCTTTACTTC	149.30462
BB93644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24415	WGS84	BB93644	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93644_Pcan_Bot2011_IGS	YD40	1	BB93644_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93644	Sample	BB93644	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD40	IGS	45	BB93644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD40	CGTAATCCTCAGTACTGGCTA	149.30462
BB93644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24415	WGS84	BB93644	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93644_Sspa_Oph2007_COI	YD40	1	BB93644_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93644	Sample	BB93644	targeted	Absent	Sample	Sspa_Oph2007_COI	YD40	COI	45	BB93644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD40	GAAGGCCTTCCGATGATC	149.30462
BB93644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24415	WGS84	BB93644	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93644_Upin_Bot2011_COI	YD40	1	BB93644_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93644	Sample	BB93644	targeted	Absent	Sample	Upin_Bot2011_COI	YD40	COI	45	BB93644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD40	CTTTAATTACAGCGTTTTTATTGTTGT	149.30462
BB93645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24561	WGS84	BB93645	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93645_Mgig_Bot2012_COI	YD38	1	BB93645_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93645	Sample	BB93645	targeted	Absent	Sample	Mgig_Bot2012_COI	YD38	COI	45	BB93645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD38	TCTTATTCGTTGGAGACTTTATAACCCT	149.30415
BB93646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24498	WGS84	BB93646	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93646_Cmae_Bot2011_COI	YD36	1	BB93646_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93646	Sample	BB93646	targeted	Absent	Sample	Cmae_Bot2011_COI	YD36	COI	45	BB93646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD36	ATGAACAGTCTATCCTCCTTTAG	149.30349
BB93646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24498	WGS84	BB93646	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93646_Msal_Bot2012_COI	YD36	1	BB93646_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93646	Sample	BB93646	targeted	Absent	Sample	Msal_Bot2012_COI	YD36	COI	45	BB93646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD36	GYTAGTTCCRATGATGTTAGCTG	149.30349
BB93648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24414	WGS84	BB93648	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93648_Aamu_Bax2006_COI	YD34	1	BB93648_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93648	Sample	BB93648	targeted	Absent	Sample	Aamu_Bax2006_COI	YD34	COI	45	BB93648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD34	GCACAACCGGGATCTTTACTTC	149.30384
BB93648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24414	WGS84	BB93648	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93648_Pcan_Bot2011_IGS	YD34	1	BB93648_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93648	Sample	BB93648	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD34	IGS	45	BB93648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD34	CGTAATCCTCAGTACTGGCTA	149.30384
BB93648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24414	WGS84	BB93648	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93648_Sspa_Oph2007_COI	YD34	1	BB93648_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93648	Sample	BB93648	targeted	Absent	Sample	Sspa_Oph2007_COI	YD34	COI	45	BB93648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD34	GAAGGCCTTCCGATGATC	149.30384
BB93648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24414	WGS84	BB93648	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93648_Upin_Bot2011_COI	YD34	1	BB93648_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93648	Sample	BB93648	targeted	Absent	Sample	Upin_Bot2011_COI	YD34	COI	45	BB93648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD34	CTTTAATTACAGCGTTTTTATTGTTGT	149.30384
BB93649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.25118	WGS84	BB93649	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93649_Mgig_Bot2012_COI	YD26	1	BB93649_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93649	Sample	BB93649	targeted	Absent	Sample	Mgig_Bot2012_COI	YD26	COI	45	BB93649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD26	TCTTATTCGTTGGAGACTTTATAACCCT	149.30754
BB93651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24831	WGS84	BB93651	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93651_Aamu_Bax2006_COI	YD22	1	BB93651_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93651	Sample	BB93651	targeted	Absent	Sample	Aamu_Bax2006_COI	YD22	COI	45	BB93651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD22	GCACAACCGGGATCTTTACTTC	149.30725
BB93651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24831	WGS84	BB93651	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93651_Pcan_Bot2011_IGS	YD22	1	BB93651_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93651	Sample	BB93651	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD22	IGS	45	BB93651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD22	CGTAATCCTCAGTACTGGCTA	149.30725
BB93651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24831	WGS84	BB93651	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93651_Sspa_Oph2007_COI	YD22	1	BB93651_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93651	Sample	BB93651	targeted	Absent	Sample	Sspa_Oph2007_COI	YD22	COI	45	BB93651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD22	GAAGGCCTTCCGATGATC	149.30725
BB93651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24831	WGS84	BB93651	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93651_Upin_Bot2011_COI	YD22	1	BB93651_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93651	Sample	BB93651	targeted	Absent	Sample	Upin_Bot2011_COI	YD22	COI	45	BB93651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD22	CTTTAATTACAGCGTTTTTATTGTTGT	149.30725
BB93652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24989	WGS84	BB93652	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93652_Mgig_Bot2012_COI	YD20	1	BB93652_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93652	Sample	BB93652	targeted	Absent	Sample	Mgig_Bot2012_COI	YD20	COI	45	BB93652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD20	TCTTATTCGTTGGAGACTTTATAACCCT	149.30652
BB93653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24764	WGS84	BB93653	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93653_Cmae_Bot2011_COI	YD19	1	BB93653_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93653	Sample	BB93653	targeted	Absent	Sample	Cmae_Bot2011_COI	YD19	COI	45	BB93653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD19	ATGAACAGTCTATCCTCCTTTAG	149.30671
BB93653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24764	WGS84	BB93653	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93653_Msal_Bot2012_COI	YD19	1	BB93653_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93653	Sample	BB93653	targeted	Absent	Sample	Msal_Bot2012_COI	YD19	COI	45	BB93653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD19	GYTAGTTCCRATGATGTTAGCTG	149.30671
BB93655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24924	WGS84	BB93655	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93655_Aamu_Bax2006_COI	YD17	1	BB93655_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93655	Sample	BB93655	targeted	Absent	Sample	Aamu_Bax2006_COI	YD17	COI	45	BB93655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD17	GCACAACCGGGATCTTTACTTC	149.30586
BB93655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24924	WGS84	BB93655	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93655_Pcan_Bot2011_IGS	YD17	1	BB93655_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93655	Sample	BB93655	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD17	IGS	45	BB93655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD17	CGTAATCCTCAGTACTGGCTA	149.30586
BB93655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24924	WGS84	BB93655	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93655_Sspa_Oph2007_COI	YD17	1	BB93655_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93655	Sample	BB93655	targeted	Absent	Sample	Sspa_Oph2007_COI	YD17	COI	45	BB93655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD17	GAAGGCCTTCCGATGATC	149.30586
BB93655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24924	WGS84	BB93655	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93655_Upin_Bot2011_COI	YD17	1	BB93655_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93655	Sample	BB93655	targeted	Absent	Sample	Upin_Bot2011_COI	YD17	COI	45	BB93655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD17	CTTTAATTACAGCGTTTTTATTGTTGT	149.30586
BB93656	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24951	WGS84	BB93656	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93656_Mgig_Bot2012_COI	YD15	1	BB93656_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93656	Sample	BB93656	targeted	Absent	Sample	Mgig_Bot2012_COI	YD15	COI	45	BB93656	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD15	TCTTATTCGTTGGAGACTTTATAACCCT	149.30338
BB93657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24873	WGS84	BB93657	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93657_Cmae_Bot2011_COI	YD14	1	BB93657_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93657	Sample	BB93657	targeted	Absent	Sample	Cmae_Bot2011_COI	YD14	COI	45	BB93657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD14	ATGAACAGTCTATCCTCCTTTAG	149.30351
BB93657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24873	WGS84	BB93657	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93657_Msal_Bot2012_COI	YD14	1	BB93657_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93657	Sample	BB93657	targeted	Absent	Sample	Msal_Bot2012_COI	YD14	COI	45	BB93657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD14	GYTAGTTCCRATGATGTTAGCTG	149.30351
BB93659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24608	WGS84	BB93659	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93659_Aamu_Bax2006_COI	YD10	1	BB93659_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93659	Sample	BB93659	targeted	Absent	Sample	Aamu_Bax2006_COI	YD10	COI	45	BB93659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD10	GCACAACCGGGATCTTTACTTC	149.30159
BB93659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24608	WGS84	BB93659	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93659_Pcan_Bot2011_IGS	YD10	1	BB93659_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93659	Sample	BB93659	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD10	IGS	45	BB93659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD10	CGTAATCCTCAGTACTGGCTA	149.30159
BB93659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24608	WGS84	BB93659	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93659_Sspa_Oph2007_COI	YD10	1	BB93659_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93659	Sample	BB93659	targeted	Absent	Sample	Sspa_Oph2007_COI	YD10	COI	45	BB93659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD10	GAAGGCCTTCCGATGATC	149.30159
BB93659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24608	WGS84	BB93659	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93659_Upin_Bot2011_COI	YD10	1	BB93659_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93659	Sample	BB93659	targeted	Absent	Sample	Upin_Bot2011_COI	YD10	COI	45	BB93659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD10	CTTTAATTACAGCGTTTTTATTGTTGT	149.30159
BB93660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24476	WGS84	BB93660	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93660_Cmae_Bot2011_COI	YD8	1	BB93660_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93660	Sample	BB93660	targeted	Absent	Sample	Cmae_Bot2011_COI	YD8	COI	45	BB93660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD8	ATGAACAGTCTATCCTCCTTTAG	149.30136
BB93660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24476	WGS84	BB93660	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93660_Msal_Bot2012_COI	YD8	1	BB93660_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93660	Sample	BB93660	targeted	Absent	Sample	Msal_Bot2012_COI	YD8	COI	45	BB93660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD8	GYTAGTTCCRATGATGTTAGCTG	149.30136
BB93662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2424	WGS84	BB93662	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93662_Aamu_Bax2006_COI	YD5	1	BB93662_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93662	Sample	BB93662	targeted	Absent	Sample	Aamu_Bax2006_COI	YD5	COI	45	BB93662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD5	GCACAACCGGGATCTTTACTTC	149.30257
BB93662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2424	WGS84	BB93662	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93662_Pcan_Bot2011_IGS	YD5	1	BB93662_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93662	Sample	BB93662	targeted	Absent	Sample	Pcan_Bot2011_IGS	YD5	IGS	45	BB93662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD5	CGTAATCCTCAGTACTGGCTA	149.30257
BB93662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2424	WGS84	BB93662	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93662_Sspa_Oph2007_COI	YD5	1	BB93662_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93662	Sample	BB93662	targeted	Absent	Sample	Sspa_Oph2007_COI	YD5	COI	45	BB93662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD5	GAAGGCCTTCCGATGATC	149.30257
BB93662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.2424	WGS84	BB93662	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93662_Upin_Bot2011_COI	YD5	1	BB93662_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93662	Sample	BB93662	targeted	Absent	Sample	Upin_Bot2011_COI	YD5	COI	45	BB93662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD5	CTTTAATTACAGCGTTTTTATTGTTGT	149.30257
BB93663	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24239	WGS84	BB93663	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93663_Mgig_Bot2012_COI	YD4	1	BB93663_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93663	Sample	BB93663	targeted	Absent	Sample	Mgig_Bot2012_COI	YD4	COI	45	BB93663	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD4	TCTTATTCGTTGGAGACTTTATAACCCT	149.30341
BB93664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24177	WGS84	BB93664	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93664_Cmae_Bot2011_COI	YD2	1	BB93664_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93664	Sample	BB93664	targeted	Absent	Sample	Cmae_Bot2011_COI	YD2	COI	45	BB93664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD2	ATGAACAGTCTATCCTCCTTTAG	149.30205
BB93664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10633	-21.24177	WGS84	BB93664	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Autumn	6/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93664_Msal_Bot2012_COI	YD2	1	BB93664_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93664	Sample	BB93664	targeted	Absent	Sample	Msal_Bot2012_COI	YD2	COI	45	BB93664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	YD2	GYTAGTTCCRATGATGTTAGCTG	149.30205
BB93666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93666	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93666_Aamu_Bax2006_COI	DD10	1	BB93666_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93666	Sample	BB93666	targeted	Absent	Sample	Aamu_Bax2006_COI	DD10	COI	45	BB93666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD10	GCACAACCGGGATCTTTACTTC	146.3657
BB93666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93666	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93666_Pcan_Bot2011_IGS	DD10	1	BB93666_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93666	Sample	BB93666	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD10	IGS	45	BB93666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD10	CGTAATCCTCAGTACTGGCTA	146.3657
BB93666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93666	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93666_Sspa_Oph2007_COI	DD10	1	BB93666_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93666	Sample	BB93666	targeted	Absent	Sample	Sspa_Oph2007_COI	DD10	COI	45	BB93666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD10	GAAGGCCTTCCGATGATC	146.3657
BB93666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18582	WGS84	BB93666	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93666_Upin_Bot2011_COI	DD10	1	BB93666_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93666	Sample	BB93666	targeted	Absent	Sample	Upin_Bot2011_COI	DD10	COI	45	BB93666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD10	CTTTAATTACAGCGTTTTTATTGTTGT	146.3657
BB93667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18664	WGS84	BB93667	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93667_Mgig_Bot2012_COI	DD8	1	BB93667_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93667	Sample	BB93667	targeted	Absent	Sample	Mgig_Bot2012_COI	DD8	COI	45	BB93667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD8	TCTTATTCGTTGGAGACTTTATAACCCT	146.36605
BB93668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18778	WGS84	BB93668	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93668_Cmae_Bot2011_COI	DD6	1	BB93668_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93668	Sample	BB93668	targeted	Absent	Sample	Cmae_Bot2011_COI	DD6	COI	45	BB93668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD6	ATGAACAGTCTATCCTCCTTTAG	146.36694
BB93668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18778	WGS84	BB93668	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93668_Msal_Bot2012_COI	DD6	1	BB93668_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93668	Sample	BB93668	targeted	Absent	Sample	Msal_Bot2012_COI	DD6	COI	45	BB93668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD6	GYTAGTTCCRATGATGTTAGCTG	146.36694
BB93670	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.1886	WGS84	BB93670	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.444	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93670_Mgig_Bot2012_COI	DD3	1	BB93670_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93670	Sample	BB93670	targeted	Present	Sample	Mgig_Bot2012_COI	DD3	COI	45	BB93670	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD3	TCTTATTCGTTGGAGACTTTATAACCCT	146.36678
BB93671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18763	WGS84	BB93671	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93671_Cmae_Bot2011_COI	DD5	1	BB93671_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93671	Sample	BB93671	targeted	Absent	Sample	Cmae_Bot2011_COI	DD5	COI	45	BB93671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD5	ATGAACAGTCTATCCTCCTTTAG	146.36571
BB93671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18763	WGS84	BB93671	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93671_Msal_Bot2012_COI	DD5	1	BB93671_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93671	Sample	BB93671	targeted	Absent	Sample	Msal_Bot2012_COI	DD5	COI	45	BB93671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD5	GYTAGTTCCRATGATGTTAGCTG	146.36571
BB93673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18359	WGS84	BB93673	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93673_Pcan_Bot2011_IGS	DD13	1	BB93673_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93673	Sample	BB93673	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD13	IGS	45	BB93673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD13	CGTAATCCTCAGTACTGGCTA	146.36547
BB93673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18359	WGS84	BB93673	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93673_Sspa_Oph2007_COI	DD13	1	BB93673_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93673	Sample	BB93673	targeted	Absent	Sample	Sspa_Oph2007_COI	DD13	COI	45	BB93673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD13	GAAGGCCTTCCGATGATC	146.36547
BB93673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18359	WGS84	BB93673	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93673_Upin_Bot2011_COI	DD13	1	BB93673_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93673	Sample	BB93673	targeted	Absent	Sample	Upin_Bot2011_COI	DD13	COI	45	BB93673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD13	CTTTAATTACAGCGTTTTTATTGTTGT	146.36547
BB93674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18474	WGS84	BB93674	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.051	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93674_Mgig_Bot2012_COI	DD12	1	BB93674_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93674	Sample	BB93674	targeted	Present	Sample	Mgig_Bot2012_COI	DD12	COI	45	BB93674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD12	TCTTATTCGTTGGAGACTTTATAACCCT	146.36414
BB93675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18176	WGS84	BB93675	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93675_Cmae_Bot2011_COI	DD17	1	BB93675_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93675	Sample	BB93675	targeted	Absent	Sample	Cmae_Bot2011_COI	DD17	COI	45	BB93675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD17	ATGAACAGTCTATCCTCCTTTAG	146.3652
BB93675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18176	WGS84	BB93675	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93675_Msal_Bot2012_COI	DD17	1	BB93675_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93675	Sample	BB93675	targeted	Absent	Sample	Msal_Bot2012_COI	DD17	COI	45	BB93675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD17	GYTAGTTCCRATGATGTTAGCTG	146.3652
BB93677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18174	WGS84	BB93677	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93677_Aamu_Bax2006_COI	DD16	1	BB93677_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93677	Sample	BB93677	targeted	Absent	Sample	Aamu_Bax2006_COI	DD16	COI	45	BB93677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD16	GCACAACCGGGATCTTTACTTC	146.36395
BB93677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18174	WGS84	BB93677	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93677_Pcan_Bot2011_IGS	DD16	1	BB93677_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93677	Sample	BB93677	targeted	Absent	Sample	Pcan_Bot2011_IGS	DD16	IGS	45	BB93677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD16	CGTAATCCTCAGTACTGGCTA	146.36395
BB93677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18174	WGS84	BB93677	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93677_Sspa_Oph2007_COI	DD16	1	BB93677_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93677	Sample	BB93677	targeted	Absent	Sample	Sspa_Oph2007_COI	DD16	COI	45	BB93677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD16	GAAGGCCTTCCGATGATC	146.36395
BB93677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18174	WGS84	BB93677	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93677_Upin_Bot2011_COI	DD16	1	BB93677_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93677	Sample	BB93677	targeted	Absent	Sample	Upin_Bot2011_COI	DD16	COI	45	BB93677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD16	CTTTAATTACAGCGTTTTTATTGTTGT	146.36395
BB93678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18188	WGS84	BB93678	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93678_Mgig_Bot2012_COI	DD18	1	BB93678_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93678	Sample	BB93678	targeted	Absent	Sample	Mgig_Bot2012_COI	DD18	COI	45	BB93678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD18	TCTTATTCGTTGGAGACTTTATAACCCT	146.36437
BB93679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18084	WGS84	BB93679	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.657	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93679_Cmae_Bot2011_COI	DD19	1	BB93679_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93679	Sample	BB93679	targeted	Present	Sample	Cmae_Bot2011_COI	DD19	COI	45	BB93679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD19	ATGAACAGTCTATCCTCCTTTAG	146.36514
BB93679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-41.18084	WGS84	BB93679	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93679_Msal_Bot2012_COI	DD19	1	BB93679_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93679	Sample	BB93679	targeted	Absent	Sample	Msal_Bot2012_COI	DD19	COI	45	BB93679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DD19	GYTAGTTCCRATGATGTTAGCTG	146.36514
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93680_Aamu_Bax2006_COI	VD5	1	BB93680_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Aamu_Bax2006_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	GCACAACCGGGATCTTTACTTC	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93680_Pcan_Bot2011_IGS	VD5	1	BB93680_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD5	IGS	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	CGTAATCCTCAGTACTGGCTA	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93680_Pper_Dia2013_COI	VD5	1	BB93680_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Pper_Dia2013_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	CTTAGTGGCATTAATTCGDAATCC	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93680_Sspa_Oph2007_COI	VD5	1	BB93680_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Sspa_Oph2007_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	GAAGGCCTTCCGATGATC	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20811	WGS84	BB93680	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93680_Upin_Bot2011_COI	VD5	1	BB93680_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Upin_Bot2011_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	CTTTAATTACAGCGTTTTTATTGTTGT	136.67224
BB93680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20811	WGS84	BB93680	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93680_Vgib_Wil2025_COI	VD5	1	BB93680_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93680	Sample	BB93680	targeted	Absent	Sample	Vgib_Wil2025_COI	VD5	COI	45	BB93680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD5	CCCCTCTTTACAAGTTCTTTAGGGT	136.67224
BB93681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8857	WGS84	BB93681	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93681_Mgig_Bot2012_COI	HD21	1	BB93681_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93681	Sample	BB93681	targeted	Absent	Sample	Mgig_Bot2012_COI	HD21	COI	45	BB93681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD21	TCTTATTCGTTGGAGACTTTATAACCCT	147.33873
BB93682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88291	WGS84	BB93682	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93682_Cmae_Bot2011_COI	HD19	1	BB93682_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93682	Sample	BB93682	targeted	Absent	Sample	Cmae_Bot2011_COI	HD19	COI	45	BB93682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD19	ATGAACAGTCTATCCTCCTTTAG	147.33838
BB93682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88291	WGS84	BB93682	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93682_Msal_Bot2012_COI	HD19	1	BB93682_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93682	Sample	BB93682	targeted	Absent	Sample	Msal_Bot2012_COI	HD19	COI	45	BB93682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD19	GYTAGTTCCRATGATGTTAGCTG	147.33838
BB93684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84382	WGS84	BB93684	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.897	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93684_Aamu_Bax2006_COI	HD7	1	BB93684_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93684	Sample	BB93684	targeted	Present	Sample	Aamu_Bax2006_COI	HD7	COI	45	BB93684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD7	GCACAACCGGGATCTTTACTTC	147.32637
BB93684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84382	WGS84	BB93684	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93684_Pcan_Bot2011_IGS	HD7	1	BB93684_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93684	Sample	BB93684	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD7	IGS	45	BB93684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD7	CGTAATCCTCAGTACTGGCTA	147.32637
BB93684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84382	WGS84	BB93684	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93684_Sspa_Oph2007_COI	HD7	1	BB93684_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93684	Sample	BB93684	targeted	Absent	Sample	Sspa_Oph2007_COI	HD7	COI	45	BB93684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD7	GAAGGCCTTCCGATGATC	147.32637
BB93684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84382	WGS84	BB93684	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93684_Upin_Bot2011_COI	HD7	1	BB93684_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93684	Sample	BB93684	targeted	Absent	Sample	Upin_Bot2011_COI	HD7	COI	45	BB93684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD7	CTTTAATTACAGCGTTTTTATTGTTGT	147.32637
BB93685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84362	WGS84	BB93685	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93685_Mgig_Bot2012_COI	HD8	1	BB93685_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93685	Sample	BB93685	targeted	Absent	Sample	Mgig_Bot2012_COI	HD8	COI	45	BB93685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD8	TCTTATTCGTTGGAGACTTTATAACCCT	147.32459
BB93686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87278	WGS84	BB93686	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93686_Cmae_Bot2011_COI	HD9	1	BB93686_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93686	Sample	BB93686	targeted	Absent	Sample	Cmae_Bot2011_COI	HD9	COI	45	BB93686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD9	ATGAACAGTCTATCCTCCTTTAG	147.33622
BB93686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87278	WGS84	BB93686	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93686_Msal_Bot2012_COI	HD9	1	BB93686_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93686	Sample	BB93686	targeted	Absent	Sample	Msal_Bot2012_COI	HD9	COI	45	BB93686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD9	GYTAGTTCCRATGATGTTAGCTG	147.33622
BB93688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8757	WGS84	BB93688	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.981	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93688_Aamu_Bax2006_COI	HD13	1	BB93688_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93688	Sample	BB93688	targeted	Present	Sample	Aamu_Bax2006_COI	HD13	COI	45	BB93688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD13	GCACAACCGGGATCTTTACTTC	147.34073
BB93688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8757	WGS84	BB93688	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93688_Pcan_Bot2011_IGS	HD13	1	BB93688_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93688	Sample	BB93688	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD13	IGS	45	BB93688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD13	CGTAATCCTCAGTACTGGCTA	147.34073
BB93688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8757	WGS84	BB93688	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93688_Sspa_Oph2007_COI	HD13	1	BB93688_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93688	Sample	BB93688	targeted	Absent	Sample	Sspa_Oph2007_COI	HD13	COI	45	BB93688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD13	GAAGGCCTTCCGATGATC	147.34073
BB93688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.8757	WGS84	BB93688	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93688_Upin_Bot2011_COI	HD13	1	BB93688_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93688	Sample	BB93688	targeted	Absent	Sample	Upin_Bot2011_COI	HD13	COI	45	BB93688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD13	CTTTAATTACAGCGTTTTTATTGTTGT	147.34073
BB93689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.87848	WGS84	BB93689	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93689_Mgig_Bot2012_COI	HD14	1	BB93689_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93689	Sample	BB93689	targeted	Absent	Sample	Mgig_Bot2012_COI	HD14	COI	45	BB93689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD14	TCTTATTCGTTGGAGACTTTATAACCCT	147.34167
BB93691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88517	WGS84	BB93691	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.394	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93691_Aamu_Bax2006_COI	HD17	1	BB93691_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93691	Sample	BB93691	targeted	Present	Sample	Aamu_Bax2006_COI	HD17	COI	45	BB93691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD17	GCACAACCGGGATCTTTACTTC	147.33462
BB93691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88517	WGS84	BB93691	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93691_Pcan_Bot2011_IGS	HD17	1	BB93691_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93691	Sample	BB93691	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD17	IGS	45	BB93691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD17	CGTAATCCTCAGTACTGGCTA	147.33462
BB93691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88517	WGS84	BB93691	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93691_Sspa_Oph2007_COI	HD17	1	BB93691_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93691	Sample	BB93691	targeted	Absent	Sample	Sspa_Oph2007_COI	HD17	COI	45	BB93691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD17	GAAGGCCTTCCGATGATC	147.33462
BB93691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.88517	WGS84	BB93691	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93691_Upin_Bot2011_COI	HD17	1	BB93691_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93691	Sample	BB93691	targeted	Absent	Sample	Upin_Bot2011_COI	HD17	COI	45	BB93691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD17	CTTTAATTACAGCGTTTTTATTGTTGT	147.33462
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20489	WGS84	BB93692	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93692_Asen_Wil2025_COI	VD20	1	BB93692_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Asen_Wil2025_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	GTTGTTGTTACAACACATGCTCTTTTG	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93692_Cjap_Sim2018_COI	VD20	1	BB93692_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Cjap_Sim2018_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93692_Esin_And2018_Cyb	VD20	1	BB93692_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Esin_And2018_Cyb	VD20	Cytb	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	ACCCCTCCTCATATCCAACCA	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93692_Mgig_Bot2012_COI	VD20	1	BB93692_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Mgig_Bot2012_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	TCTTATTCGTTGGAGACTTTATAACCCT	136.68229
BB93692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20489	WGS84	BB93692	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93692_Mjap_Gib2022_COI	VD20	1	BB93692_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93692	Sample	BB93692	targeted	Absent	Sample	Mjap_Gib2022_COI	VD20	COI	45	BB93692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD20	AATTTAATTGTGACTAGGCATGGTCTG	136.68229
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93693_Cmae_Bot2011_COI	VD10	1	BB93693_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Cmae_Bot2011_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	ATGAACAGTCTATCCTCCTTTAG	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93693_Hsan_Wil2021_COI	VD10	1	BB93693_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Hsan_Wil2021_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	CCTGGGCCGGTATAGTAGGT	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93693_Mros_Gib2022_COI	VD10	1	BB93693_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Mros_Gib2022_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93693_Msal_Bot2012_COI	VD10	1	BB93693_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Msal_Bot2012_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	GYTAGTTCCRATGATGTTAGCTG	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93693_Pvir_Dia2013_COI	VD10	1	BB93693_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Pvir_Dia2013_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	CTTAGTGGCATTAATTCGDAATCC	136.66871
BB93693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20575	WGS84	BB93693	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93693_Rhar_Sim2018_COI	VD10	1	BB93693_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93693	Sample	BB93693	targeted	Absent	Sample	Rhar_Sim2018_COI	VD10	COI	45	BB93693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD10	CTGCTGCTATTGCTCATG	136.66871
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93695_Aamu_Bax2006_COI	VD2	1	BB93695_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Aamu_Bax2006_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	GCACAACCGGGATCTTTACTTC	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93695_Pcan_Bot2011_IGS	VD2	1	BB93695_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD2	IGS	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	CGTAATCCTCAGTACTGGCTA	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93695_Pper_Dia2013_COI	VD2	1	BB93695_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Pper_Dia2013_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	CTTAGTGGCATTAATTCGDAATCC	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93695_Sspa_Oph2007_COI	VD2	1	BB93695_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Sspa_Oph2007_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	GAAGGCCTTCCGATGATC	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.19543	WGS84	BB93695	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93695_Upin_Bot2011_COI	VD2	1	BB93695_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Upin_Bot2011_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	CTTTAATTACAGCGTTTTTATTGTTGT	136.68489
BB93695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.19543	WGS84	BB93695	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93695_Vgib_Wil2025_COI	VD2	1	BB93695_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93695	Sample	BB93695	targeted	Absent	Sample	Vgib_Wil2025_COI	VD2	COI	45	BB93695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD2	CCCCTCTTTACAAGTTCTTTAGGGT	136.68489
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.2061	WGS84	BB93696	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93696_Asen_Wil2025_COI	VD3	1	BB93696_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Asen_Wil2025_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	GTTGTTGTTACAACACATGCTCTTTTG	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93696_Cjap_Sim2018_COI	VD3	1	BB93696_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Cjap_Sim2018_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93696_Esin_And2018_Cyb	VD3	1	BB93696_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Esin_And2018_Cyb	VD3	Cytb	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	ACCCCTCCTCATATCCAACCA	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93696_Mgig_Bot2012_COI	VD3	1	BB93696_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Mgig_Bot2012_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	TCTTATTCGTTGGAGACTTTATAACCCT	136.68091
BB93696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.2061	WGS84	BB93696	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93696_Mjap_Gib2022_COI	VD3	1	BB93696_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93696	Sample	BB93696	targeted	Absent	Sample	Mjap_Gib2022_COI	VD3	COI	45	BB93696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD3	AATTTAATTGTGACTAGGCATGGTCTG	136.68091
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93697_Cmae_Bot2011_COI	VD4	1	BB93697_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Cmae_Bot2011_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	ATGAACAGTCTATCCTCCTTTAG	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93697_Hsan_Wil2021_COI	VD4	1	BB93697_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Hsan_Wil2021_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	CCTGGGCCGGTATAGTAGGT	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93697_Mros_Gib2022_COI	VD4	1	BB93697_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Mros_Gib2022_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93697_Msal_Bot2012_COI	VD4	1	BB93697_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Msal_Bot2012_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	GYTAGTTCCRATGATGTTAGCTG	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93697_Pvir_Dia2013_COI	VD4	1	BB93697_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Pvir_Dia2013_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	CTTAGTGGCATTAATTCGDAATCC	136.67967
BB93697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20645	WGS84	BB93697	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93697_Rhar_Sim2018_COI	VD4	1	BB93697_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93697	Sample	BB93697	targeted	Absent	Sample	Rhar_Sim2018_COI	VD4	COI	45	BB93697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD4	CTGCTGCTATTGCTCATG	136.67967
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93699_Aamu_Bax2006_COI	VD16	1	BB93699_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Aamu_Bax2006_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	GCACAACCGGGATCTTTACTTC	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93699_Pcan_Bot2011_IGS	VD16	1	BB93699_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD16	IGS	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	CGTAATCCTCAGTACTGGCTA	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93699_Pper_Dia2013_COI	VD16	1	BB93699_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Pper_Dia2013_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	CTTAGTGGCATTAATTCGDAATCC	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93699_Sspa_Oph2007_COI	VD16	1	BB93699_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Sspa_Oph2007_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	GAAGGCCTTCCGATGATC	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20703	WGS84	BB93699	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93699_Upin_Bot2011_COI	VD16	1	BB93699_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Upin_Bot2011_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	CTTTAATTACAGCGTTTTTATTGTTGT	136.66813
BB93699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20703	WGS84	BB93699	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93699_Vgib_Wil2025_COI	VD16	1	BB93699_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93699	Sample	BB93699	targeted	Absent	Sample	Vgib_Wil2025_COI	VD16	COI	45	BB93699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD16	CCCCTCTTTACAAGTTCTTTAGGGT	136.66813
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.21016	WGS84	BB93700	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93700_Asen_Wil2025_COI	VD14	1	BB93700_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Asen_Wil2025_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	GTTGTTGTTACAACACATGCTCTTTTG	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93700_Cjap_Sim2018_COI	VD14	1	BB93700_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Cjap_Sim2018_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93700_Esin_And2018_Cyb	VD14	1	BB93700_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Esin_And2018_Cyb	VD14	Cytb	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	ACCCCTCCTCATATCCAACCA	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93700_Mgig_Bot2012_COI	VD14	1	BB93700_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Mgig_Bot2012_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	TCTTATTCGTTGGAGACTTTATAACCCT	136.67151
BB93700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.21016	WGS84	BB93700	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93700_Mjap_Gib2022_COI	VD14	1	BB93700_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93700	Sample	BB93700	targeted	Absent	Sample	Mjap_Gib2022_COI	VD14	COI	45	BB93700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD14	AATTTAATTGTGACTAGGCATGGTCTG	136.67151
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93701_Cmae_Bot2011_COI	VD13	1	BB93701_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Cmae_Bot2011_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	ATGAACAGTCTATCCTCCTTTAG	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93701_Hsan_Wil2021_COI	VD13	1	BB93701_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Hsan_Wil2021_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	CCTGGGCCGGTATAGTAGGT	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93701_Mros_Gib2022_COI	VD13	1	BB93701_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Mros_Gib2022_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93701_Msal_Bot2012_COI	VD13	1	BB93701_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Msal_Bot2012_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	GYTAGTTCCRATGATGTTAGCTG	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93701_Pvir_Dia2013_COI	VD13	1	BB93701_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Pvir_Dia2013_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	CTTAGTGGCATTAATTCGDAATCC	136.67057
BB93701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20918	WGS84	BB93701	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93701_Rhar_Sim2018_COI	VD13	1	BB93701_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93701	Sample	BB93701	targeted	Absent	Sample	Rhar_Sim2018_COI	VD13	COI	45	BB93701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD13	CTGCTGCTATTGCTCATG	136.67057
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93703_Aamu_Bax2006_COI	VD8	1	BB93703_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Aamu_Bax2006_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	GCACAACCGGGATCTTTACTTC	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93703_Pcan_Bot2011_IGS	VD8	1	BB93703_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Pcan_Bot2011_IGS	VD8	IGS	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	CGTAATCCTCAGTACTGGCTA	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93703_Pper_Dia2013_COI	VD8	1	BB93703_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Pper_Dia2013_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	CTTAGTGGCATTAATTCGDAATCC	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93703_Sspa_Oph2007_COI	VD8	1	BB93703_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Sspa_Oph2007_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	GAAGGCCTTCCGATGATC	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20367	WGS84	BB93703	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93703_Upin_Bot2011_COI	VD8	1	BB93703_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Upin_Bot2011_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	CTTTAATTACAGCGTTTTTATTGTTGT	136.6702
BB93703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.20367	WGS84	BB93703	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93703_Vgib_Wil2025_COI	VD8	1	BB93703_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93703	Sample	BB93703	targeted	Absent	Sample	Vgib_Wil2025_COI	VD8	COI	45	BB93703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD8	CCCCTCTTTACAAGTTCTTTAGGGT	136.6702
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.204	WGS84	BB93704	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93704_Asen_Wil2025_COI	VD7	1	BB93704_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Asen_Wil2025_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	GTTGTTGTTACAACACATGCTCTTTTG	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93704_Cjap_Sim2018_COI	VD7	1	BB93704_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Cjap_Sim2018_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	TTAATATACGGTCATTTGGTATGAGTATAGATC	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93704_Esin_And2018_Cyb	VD7	1	BB93704_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Esin_And2018_Cyb	VD7	Cytb	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	ACCCCTCCTCATATCCAACCA	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93704_Mgig_Bot2012_COI	VD7	1	BB93704_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Mgig_Bot2012_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	TCTTATTCGTTGGAGACTTTATAACCCT	136.67046
BB93704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.204	WGS84	BB93704	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93704_Mjap_Gib2022_COI	VD7	1	BB93704_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93704	Sample	BB93704	targeted	Absent	Sample	Mjap_Gib2022_COI	VD7	COI	45	BB93704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD7	AATTTAATTGTGACTAGGCATGGTCTG	136.67046
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93705_Cmae_Bot2011_COI	VD6	1	BB93705_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Cmae_Bot2011_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	ATGAACAGTCTATCCTCCTTTAG	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93705_Hsan_Wil2021_COI	VD6	1	BB93705_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Hsan_Wil2021_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	CCTGGGCCGGTATAGTAGGT	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93705_Mros_Gib2022_COI	VD6	1	BB93705_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Mros_Gib2022_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	ACTTCTACCTCCTGCTCTTCTACTTCTC	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93705_Msal_Bot2012_COI	VD6	1	BB93705_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Msal_Bot2012_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	GYTAGTTCCRATGATGTTAGCTG	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93705_Pvir_Dia2013_COI	VD6	1	BB93705_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Pvir_Dia2013_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	CTTAGTGGCATTAATTCGDAATCC	136.67185
BB93705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.20707	WGS84	BB93705	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Gove_Autumn	25/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93705_Rhar_Sim2018_COI	VD6	1	BB93705_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93705	Sample	BB93705	targeted	Absent	Sample	Rhar_Sim2018_COI	VD6	COI	45	BB93705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	VD6	CTGCTGCTATTGCTCATG	136.67185
BB93707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84389	WGS84	BB93707	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.318	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93707_Aamu_Bax2006_COI	HD4	1	BB93707_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93707	Sample	BB93707	targeted	Present	Sample	Aamu_Bax2006_COI	HD4	COI	45	BB93707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD4	GCACAACCGGGATCTTTACTTC	147.33123
BB93707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84389	WGS84	BB93707	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93707_Pcan_Bot2011_IGS	HD4	1	BB93707_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93707	Sample	BB93707	targeted	Absent	Sample	Pcan_Bot2011_IGS	HD4	IGS	45	BB93707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD4	CGTAATCCTCAGTACTGGCTA	147.33123
BB93707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84389	WGS84	BB93707	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93707_Sspa_Oph2007_COI	HD4	1	BB93707_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93707	Sample	BB93707	targeted	Absent	Sample	Sspa_Oph2007_COI	HD4	COI	45	BB93707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD4	GAAGGCCTTCCGATGATC	147.33123
BB93707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.84389	WGS84	BB93707	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93707_Upin_Bot2011_COI	HD4	1	BB93707_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93707	Sample	BB93707	targeted	Absent	Sample	Upin_Bot2011_COI	HD4	COI	45	BB93707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD4	CTTTAATTACAGCGTTTTTATTGTTGT	147.33123
BB93708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82814	WGS84	BB93708	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93708_Mgig_Bot2012_COI	HD3	1	BB93708_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93708	Sample	BB93708	targeted	Absent	Sample	Mgig_Bot2012_COI	HD3	COI	45	BB93708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD3	TCTTATTCGTTGGAGACTTTATAACCCT	147.31543
BB93709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82857	WGS84	BB93709	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.558	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93709_Cmae_Bot2011_COI	HD2	1	BB93709_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93709	Sample	BB93709	targeted	Present	Sample	Cmae_Bot2011_COI	HD2	COI	45	BB93709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD2	ATGAACAGTCTATCCTCCTTTAG	147.3172
BB93709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10631	-42.82857	WGS84	BB93709	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93709_Msal_Bot2012_COI	HD2	1	BB93709_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93709	Sample	BB93709	targeted	Absent	Sample	Msal_Bot2012_COI	HD2	COI	45	BB93709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HD2	GYTAGTTCCRATGATGTTAGCTG	147.3172
BB93710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67457	WGS84	BB93710	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93710_Aamu_Bax2006_COI	WD15	1	BB93710_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93710	Sample	BB93710	targeted	Absent	Sample	Aamu_Bax2006_COI	WD15	COI	45	BB93710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD15	GCACAACCGGGATCTTTACTTC	141.87024
BB93710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67457	WGS84	BB93710	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93710_Pcan_Bot2011_IGS	WD15	1	BB93710_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93710	Sample	BB93710	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD15	IGS	45	BB93710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD15	CGTAATCCTCAGTACTGGCTA	141.87024
BB93710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67457	WGS84	BB93710	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93710_Sspa_Oph2007_COI	WD15	1	BB93710_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93710	Sample	BB93710	targeted	Absent	Sample	Sspa_Oph2007_COI	WD15	COI	45	BB93710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD15	GAAGGCCTTCCGATGATC	141.87024
BB93710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67457	WGS84	BB93710	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93710_Upin_Bot2011_COI	WD15	1	BB93710_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93710	Sample	BB93710	targeted	Absent	Sample	Upin_Bot2011_COI	WD15	COI	45	BB93710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD15	CTTTAATTACAGCGTTTTTATTGTTGT	141.87024
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66717	WGS84	BB93711	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93711_Asen_Wil2025_COI	WD1	1	BB93711_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Asen_Wil2025_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	GTTGTTGTTACAACACATGCTCTTTTG	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93711_Cjap_Sim2018_COI	WD1	1	BB93711_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Cjap_Sim2018_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93711_Esin_And2018_Cyb	WD1	1	BB93711_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Esin_And2018_Cyb	WD1	Cytb	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	ACCCCTCCTCATATCCAACCA	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93711_Mgig_Bot2012_COI	WD1	1	BB93711_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Mgig_Bot2012_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	TCTTATTCGTTGGAGACTTTATAACCCT	141.84674
BB93711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66717	WGS84	BB93711	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93711_Mjap_Gib2022_COI	WD1	1	BB93711_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93711	Sample	BB93711	targeted	Absent	Sample	Mjap_Gib2022_COI	WD1	COI	45	BB93711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD1	AATTTAATTGTGACTAGGCATGGTCTG	141.84674
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93712_Cmae_Bot2011_COI	WD3	1	BB93712_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Cmae_Bot2011_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	ATGAACAGTCTATCCTCCTTTAG	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93712_Hsan_Wil2021_COI	WD3	1	BB93712_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Hsan_Wil2021_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	CCTGGGCCGGTATAGTAGGT	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93712_Mros_Gib2022_COI	WD3	1	BB93712_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Mros_Gib2022_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93712_Msal_Bot2012_COI	WD3	1	BB93712_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Msal_Bot2012_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	GYTAGTTCCRATGATGTTAGCTG	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93712_Pvir_Dia2013_COI	WD3	1	BB93712_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Pvir_Dia2013_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	CTTAGTGGCATTAATTCGDAATCC	141.84936
BB93712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66714	WGS84	BB93712	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93712_Rhar_Sim2018_COI	WD3	1	BB93712_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93712	Sample	BB93712	targeted	Absent	Sample	Rhar_Sim2018_COI	WD3	COI	45	BB93712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD3	CTGCTGCTATTGCTCATG	141.84936
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93714_Aamu_Bax2006_COI	WD7	1	BB93714_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Aamu_Bax2006_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	GCACAACCGGGATCTTTACTTC	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93714_Pcan_Bot2011_IGS	WD7	1	BB93714_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD7	IGS	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	CGTAATCCTCAGTACTGGCTA	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93714_Pper_Dia2013_COI	WD7	1	BB93714_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Pper_Dia2013_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	CTTAGTGGCATTAATTCGDAATCC	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93714_Sspa_Oph2007_COI	WD7	1	BB93714_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Sspa_Oph2007_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	GAAGGCCTTCCGATGATC	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66878	WGS84	BB93714	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93714_Upin_Bot2011_COI	WD7	1	BB93714_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Upin_Bot2011_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	CTTTAATTACAGCGTTTTTATTGTTGT	141.86098
BB93714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66878	WGS84	BB93714	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93714_Vgib_Wil2025_COI	WD7	1	BB93714_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93714	Sample	BB93714	targeted	Absent	Sample	Vgib_Wil2025_COI	WD7	COI	45	BB93714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD7	CCCCTCTTTACAAGTTCTTTAGGGT	141.86098
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.66989	WGS84	BB93715	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93715_Asen_Wil2025_COI	WD8	1	BB93715_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Asen_Wil2025_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	GTTGTTGTTACAACACATGCTCTTTTG	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93715_Cjap_Sim2018_COI	WD8	1	BB93715_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Cjap_Sim2018_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93715_Esin_And2018_Cyb	WD8	1	BB93715_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Esin_And2018_Cyb	WD8	Cytb	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	ACCCCTCCTCATATCCAACCA	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93715_Mgig_Bot2012_COI	WD8	1	BB93715_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Mgig_Bot2012_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	TCTTATTCGTTGGAGACTTTATAACCCT	141.86241
BB93715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66989	WGS84	BB93715	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93715_Mjap_Gib2022_COI	WD8	1	BB93715_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93715	Sample	BB93715	targeted	Absent	Sample	Mjap_Gib2022_COI	WD8	COI	45	BB93715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD8	AATTTAATTGTGACTAGGCATGGTCTG	141.86241
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93716_Cmae_Bot2011_COI	WD9	1	BB93716_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Cmae_Bot2011_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	ATGAACAGTCTATCCTCCTTTAG	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93716_Hsan_Wil2021_COI	WD9	1	BB93716_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Hsan_Wil2021_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	CCTGGGCCGGTATAGTAGGT	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BB93716_Mros_Gib2022_COI	WD9	1	BB93716_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Mros_Gib2022_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93716_Msal_Bot2012_COI	WD9	1	BB93716_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Msal_Bot2012_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	GYTAGTTCCRATGATGTTAGCTG	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BB93716_Pvir_Dia2013_COI	WD9	1	BB93716_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Pvir_Dia2013_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	CTTAGTGGCATTAATTCGDAATCC	141.86396
BB93716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66985	WGS84	BB93716	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BB93716_Rhar_Sim2018_COI	WD9	1	BB93716_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93716	Sample	BB93716	targeted	Absent	Sample	Rhar_Sim2018_COI	WD9	COI	45	BB93716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD9	CTGCTGCTATTGCTCATG	141.86396
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93718_Aamu_Bax2006_COI	WD12	1	BB93718_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Aamu_Bax2006_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	GCACAACCGGGATCTTTACTTC	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93718_Pcan_Bot2011_IGS	WD12	1	BB93718_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD12	IGS	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	CGTAATCCTCAGTACTGGCTA	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BB93718_Pper_Dia2013_COI	WD12	1	BB93718_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Pper_Dia2013_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	CTTAGTGGCATTAATTCGDAATCC	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93718_Sspa_Oph2007_COI	WD12	1	BB93718_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Sspa_Oph2007_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	GAAGGCCTTCCGATGATC	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67373	WGS84	BB93718	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93718_Upin_Bot2011_COI	WD12	1	BB93718_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Upin_Bot2011_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	CTTTAATTACAGCGTTTTTATTGTTGT	141.86683
BB93718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67373	WGS84	BB93718	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BB93718_Vgib_Wil2025_COI	WD12	1	BB93718_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93718	Sample	BB93718	targeted	Absent	Sample	Vgib_Wil2025_COI	WD12	COI	45	BB93718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD12	CCCCTCTTTACAAGTTCTTTAGGGT	141.86683
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-12.67349	WGS84	BB93719	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BB93719_Asen_Wil2025_COI	WD14	1	BB93719_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Asen_Wil2025_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	GTTGTTGTTACAACACATGCTCTTTTG	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BB93719_Cjap_Sim2018_COI	WD14	1	BB93719_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Cjap_Sim2018_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BB93719_Esin_And2018_Cyb	WD14	1	BB93719_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Esin_And2018_Cyb	WD14	Cytb	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	ACCCCTCCTCATATCCAACCA	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93719_Mgig_Bot2012_COI	WD14	1	BB93719_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Mgig_Bot2012_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	TCTTATTCGTTGGAGACTTTATAACCCT	141.86939
BB93719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67349	WGS84	BB93719	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BB93719_Mjap_Gib2022_COI	WD14	1	BB93719_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93719	Sample	BB93719	targeted	Absent	Sample	Mjap_Gib2022_COI	WD14	COI	45	BB93719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD14	AATTTAATTGTGACTAGGCATGGTCTG	141.86939
BB93721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67454	WGS84	BB93721	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93721_Aamu_Bax2006_COI	WD18	1	BB93721_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93721	Sample	BB93721	targeted	Absent	Sample	Aamu_Bax2006_COI	WD18	COI	45	BB93721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD18	GCACAACCGGGATCTTTACTTC	141.87152
BB93721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67454	WGS84	BB93721	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93721_Pcan_Bot2011_IGS	WD18	1	BB93721_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93721	Sample	BB93721	targeted	Absent	Sample	Pcan_Bot2011_IGS	WD18	IGS	45	BB93721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD18	CGTAATCCTCAGTACTGGCTA	141.87152
BB93721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67454	WGS84	BB93721	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93721_Sspa_Oph2007_COI	WD18	1	BB93721_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93721	Sample	BB93721	targeted	Absent	Sample	Sspa_Oph2007_COI	WD18	COI	45	BB93721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD18	GAAGGCCTTCCGATGATC	141.87152
BB93721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67454	WGS84	BB93721	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93721_Upin_Bot2011_COI	WD18	1	BB93721_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93721	Sample	BB93721	targeted	Absent	Sample	Upin_Bot2011_COI	WD18	COI	45	BB93721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD18	CTTTAATTACAGCGTTTTTATTGTTGT	141.87152
BB93722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.67312	WGS84	BB93722	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	22/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93722_Mgig_Bot2012_COI	WD19	1	BB93722_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93722	Sample	BB93722	targeted	Absent	Sample	Mgig_Bot2012_COI	WD19	COI	45	BB93722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD19	TCTTATTCGTTGGAGACTTTATAACCCT	141.87125
BB93723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66582	WGS84	BB93723	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93723_Cmae_Bot2011_COI	WD5	1	BB93723_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93723	Sample	BB93723	targeted	Absent	Sample	Cmae_Bot2011_COI	WD5	COI	45	BB93723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD5	ATGAACAGTCTATCCTCCTTTAG	141.85
BB93723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10636	-12.66582	WGS84	BB93723	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Autumn	21/05/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93723_Msal_Bot2012_COI	WD5	1	BB93723_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93723	Sample	BB93723	targeted	Absent	Sample	Msal_Bot2012_COI	WD5	COI	45	BB93723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WD5	GYTAGTTCCRATGATGTTAGCTG	141.85
BB93725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81853	WGS84	BB93725	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.504	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93725_Aamu_Bax2006_COI	MC75	1	BB93725_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93725	Sample	BB93725	targeted	Present	Sample	Aamu_Bax2006_COI	MC75	COI	45	BB93725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC75	GCACAACCGGGATCTTTACTTC	144.92784
BB93725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81853	WGS84	BB93725	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93725_Pcan_Bot2011_IGS	MC75	1	BB93725_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93725	Sample	BB93725	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC75	IGS	45	BB93725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC75	CGTAATCCTCAGTACTGGCTA	144.92784
BB93725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81853	WGS84	BB93725	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.804	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93725_Sspa_Oph2007_COI	MC75	1	BB93725_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93725	Sample	BB93725	targeted	Present	Sample	Sspa_Oph2007_COI	MC75	COI	45	BB93725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC75	GAAGGCCTTCCGATGATC	144.92784
BB93725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81853	WGS84	BB93725	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93725_Upin_Bot2011_COI	MC75	1	BB93725_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93725	Sample	BB93725	targeted	Absent	Sample	Upin_Bot2011_COI	MC75	COI	45	BB93725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC75	CTTTAATTACAGCGTTTTTATTGTTGT	144.92784
BB93726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81884	WGS84	BB93726	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.752	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93726_Mgig_Bot2012_COI	MC70	1	BB93726_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93726	Sample	BB93726	targeted	Present	Sample	Mgig_Bot2012_COI	MC70	COI	45	BB93726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC70	TCTTATTCGTTGGAGACTTTATAACCCT	144.92457
BB93727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82836	WGS84	BB93727	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93727_Cmae_Bot2011_COI	MC50	1	BB93727_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93727	Sample	BB93727	targeted	Absent	Sample	Cmae_Bot2011_COI	MC50	COI	45	BB93727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC50	ATGAACAGTCTATCCTCCTTTAG	144.89947
BB93727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82836	WGS84	BB93727	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93727_Msal_Bot2012_COI	MC50	1	BB93727_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93727	Sample	BB93727	targeted	Absent	Sample	Msal_Bot2012_COI	MC50	COI	45	BB93727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC50	GYTAGTTCCRATGATGTTAGCTG	144.89947
BB93729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86215	WGS84	BB93729	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93729_Aamu_Bax2006_COI	MC40	1	BB93729_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93729	Sample	BB93729	targeted	Absent	Sample	Aamu_Bax2006_COI	MC40	COI	45	BB93729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC40	GCACAACCGGGATCTTTACTTC	144.91262
BB93729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86215	WGS84	BB93729	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93729_Pcan_Bot2011_IGS	MC40	1	BB93729_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93729	Sample	BB93729	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC40	IGS	45	BB93729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC40	CGTAATCCTCAGTACTGGCTA	144.91262
BB93729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86215	WGS84	BB93729	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.129	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93729_Sspa_Oph2007_COI	MC40	1	BB93729_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93729	Sample	BB93729	targeted	Present	Sample	Sspa_Oph2007_COI	MC40	COI	45	BB93729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC40	GAAGGCCTTCCGATGATC	144.91262
BB93729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86215	WGS84	BB93729	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93729_Upin_Bot2011_COI	MC40	1	BB93729_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93729	Sample	BB93729	targeted	Absent	Sample	Upin_Bot2011_COI	MC40	COI	45	BB93729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC40	CTTTAATTACAGCGTTTTTATTGTTGT	144.91262
BB93730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84891	WGS84	BB93730	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93730_Cmae_Bot2011_COI	MC20	1	BB93730_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93730	Sample	BB93730	targeted	Absent	Sample	Cmae_Bot2011_COI	MC20	COI	45	BB93730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC20	ATGAACAGTCTATCCTCCTTTAG	144.91502
BB93730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84891	WGS84	BB93730	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93730_Msal_Bot2012_COI	MC20	1	BB93730_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93730	Sample	BB93730	targeted	Absent	Sample	Msal_Bot2012_COI	MC20	COI	45	BB93730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC20	GYTAGTTCCRATGATGTTAGCTG	144.91502
BB93732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81862	WGS84	BB93732	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93732_Aamu_Bax2006_COI	MC85	1	BB93732_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93732	Sample	BB93732	targeted	Absent	Sample	Aamu_Bax2006_COI	MC85	COI	45	BB93732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC85	GCACAACCGGGATCTTTACTTC	144.92195
BB93732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81862	WGS84	BB93732	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93732_Pcan_Bot2011_IGS	MC85	1	BB93732_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93732	Sample	BB93732	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC85	IGS	45	BB93732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC85	CGTAATCCTCAGTACTGGCTA	144.92195
BB93732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81862	WGS84	BB93732	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.82	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93732_Sspa_Oph2007_COI	MC85	1	BB93732_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93732	Sample	BB93732	targeted	Present	Sample	Sspa_Oph2007_COI	MC85	COI	45	BB93732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC85	GAAGGCCTTCCGATGATC	144.92195
BB93732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81862	WGS84	BB93732	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93732_Upin_Bot2011_COI	MC85	1	BB93732_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93732	Sample	BB93732	targeted	Absent	Sample	Upin_Bot2011_COI	MC85	COI	45	BB93732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC85	CTTTAATTACAGCGTTTTTATTGTTGT	144.92195
BB93733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84169	WGS84	BB93733	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.005	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93733_Mgig_Bot2012_COI	MC5	1	BB93733_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93733	Sample	BB93733	targeted	Present	Sample	Mgig_Bot2012_COI	MC5	COI	45	BB93733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC5	TCTTATTCGTTGGAGACTTTATAACCCT	144.92949
BB93734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82131	WGS84	BB93734	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93734_Cmae_Bot2011_COI	MC115	1	BB93734_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93734	Sample	BB93734	targeted	Absent	Sample	Cmae_Bot2011_COI	MC115	COI	45	BB93734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC115	ATGAACAGTCTATCCTCCTTTAG	144.90843
BB93734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82131	WGS84	BB93734	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93734_Msal_Bot2012_COI	MC115	1	BB93734_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93734	Sample	BB93734	targeted	Absent	Sample	Msal_Bot2012_COI	MC115	COI	45	BB93734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC115	GYTAGTTCCRATGATGTTAGCTG	144.90843
BB93736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81673	WGS84	BB93736	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93736_Aamu_Bax2006_COI	MC78	1	BB93736_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93736	Sample	BB93736	targeted	Absent	Sample	Aamu_Bax2006_COI	MC78	COI	45	BB93736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC78	GCACAACCGGGATCTTTACTTC	144.92566
BB93736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81673	WGS84	BB93736	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93736_Pcan_Bot2011_IGS	MC78	1	BB93736_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93736	Sample	BB93736	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC78	IGS	45	BB93736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC78	CGTAATCCTCAGTACTGGCTA	144.92566
BB93736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81673	WGS84	BB93736	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.688	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93736_Sspa_Oph2007_COI	MC78	1	BB93736_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93736	Sample	BB93736	targeted	Present	Sample	Sspa_Oph2007_COI	MC78	COI	45	BB93736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC78	GAAGGCCTTCCGATGATC	144.92566
BB93736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81673	WGS84	BB93736	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93736_Upin_Bot2011_COI	MC78	1	BB93736_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93736	Sample	BB93736	targeted	Absent	Sample	Upin_Bot2011_COI	MC78	COI	45	BB93736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC78	CTTTAATTACAGCGTTTTTATTGTTGT	144.92566
BB93737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81959	WGS84	BB93737	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.081	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93737_Mgig_Bot2012_COI	MC77	1	BB93737_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93737	Sample	BB93737	targeted	Present	Sample	Mgig_Bot2012_COI	MC77	COI	45	BB93737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC77	TCTTATTCGTTGGAGACTTTATAACCCT	144.92662
BB93738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81989	WGS84	BB93738	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93738_Cmae_Bot2011_COI	MC73	1	BB93738_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93738	Sample	BB93738	targeted	Absent	Sample	Cmae_Bot2011_COI	MC73	COI	45	BB93738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC73	ATGAACAGTCTATCCTCCTTTAG	144.92932
BB93738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81989	WGS84	BB93738	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93738_Msal_Bot2012_COI	MC73	1	BB93738_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93738	Sample	BB93738	targeted	Absent	Sample	Msal_Bot2012_COI	MC73	COI	45	BB93738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC73	GYTAGTTCCRATGATGTTAGCTG	144.92932
BB93740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81812	WGS84	BB93740	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.092	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93740_Mgig_Bot2012_COI	MC68	1	BB93740_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93740	Sample	BB93740	targeted	Present	Sample	Mgig_Bot2012_COI	MC68	COI	45	BB93740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC68	TCTTATTCGTTGGAGACTTTATAACCCT	144.9219
BB93741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81929	WGS84	BB93741	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93741_Cmae_Bot2011_COI	MC66	1	BB93741_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93741	Sample	BB93741	targeted	Absent	Sample	Cmae_Bot2011_COI	MC66	COI	45	BB93741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC66	ATGAACAGTCTATCCTCCTTTAG	144.91866
BB93741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81929	WGS84	BB93741	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93741_Msal_Bot2012_COI	MC66	1	BB93741_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93741	Sample	BB93741	targeted	Absent	Sample	Msal_Bot2012_COI	MC66	COI	45	BB93741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC66	GYTAGTTCCRATGATGTTAGCTG	144.91866
BB93743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82302	WGS84	BB93743	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93743_Aamu_Bax2006_COI	MC62	1	BB93743_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93743	Sample	BB93743	targeted	Absent	Sample	Aamu_Bax2006_COI	MC62	COI	45	BB93743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC62	GCACAACCGGGATCTTTACTTC	144.90666
BB93743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82302	WGS84	BB93743	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93743_Pcan_Bot2011_IGS	MC62	1	BB93743_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93743	Sample	BB93743	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC62	IGS	45	BB93743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC62	CGTAATCCTCAGTACTGGCTA	144.90666
BB93743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82302	WGS84	BB93743	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.151	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93743_Sspa_Oph2007_COI	MC62	1	BB93743_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93743	Sample	BB93743	targeted	Present	Sample	Sspa_Oph2007_COI	MC62	COI	45	BB93743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC62	GAAGGCCTTCCGATGATC	144.90666
BB93743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82302	WGS84	BB93743	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93743_Upin_Bot2011_COI	MC62	1	BB93743_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93743	Sample	BB93743	targeted	Absent	Sample	Upin_Bot2011_COI	MC62	COI	45	BB93743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC62	CTTTAATTACAGCGTTTTTATTGTTGT	144.90666
BB93744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82503	WGS84	BB93744	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.753	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93744_Mgig_Bot2012_COI	MC54	1	BB93744_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93744	Sample	BB93744	targeted	Present	Sample	Mgig_Bot2012_COI	MC54	COI	45	BB93744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC54	TCTTATTCGTTGGAGACTTTATAACCCT	144.9036
BB93745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82512	WGS84	BB93745	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93745_Cmae_Bot2011_COI	MC53	1	BB93745_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93745	Sample	BB93745	targeted	Absent	Sample	Cmae_Bot2011_COI	MC53	COI	45	BB93745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC53	ATGAACAGTCTATCCTCCTTTAG	144.90113
BB93745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82512	WGS84	BB93745	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93745_Msal_Bot2012_COI	MC53	1	BB93745_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93745	Sample	BB93745	targeted	Absent	Sample	Msal_Bot2012_COI	MC53	COI	45	BB93745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC53	GYTAGTTCCRATGATGTTAGCTG	144.90113
BB93747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8609	WGS84	BB93747	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93747_Aamu_Bax2006_COI	MC48	1	BB93747_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93747	Sample	BB93747	targeted	Absent	Sample	Aamu_Bax2006_COI	MC48	COI	45	BB93747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC48	GCACAACCGGGATCTTTACTTC	144.91203
BB93747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8609	WGS84	BB93747	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93747_Pcan_Bot2011_IGS	MC48	1	BB93747_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93747	Sample	BB93747	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC48	IGS	45	BB93747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC48	CGTAATCCTCAGTACTGGCTA	144.91203
BB93747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8609	WGS84	BB93747	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.16	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93747_Sspa_Oph2007_COI	MC48	1	BB93747_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93747	Sample	BB93747	targeted	Present	Sample	Sspa_Oph2007_COI	MC48	COI	45	BB93747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC48	GAAGGCCTTCCGATGATC	144.91203
BB93747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.8609	WGS84	BB93747	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93747_Upin_Bot2011_COI	MC48	1	BB93747_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93747	Sample	BB93747	targeted	Absent	Sample	Upin_Bot2011_COI	MC48	COI	45	BB93747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC48	CTTTAATTACAGCGTTTTTATTGTTGT	144.91203
BB93748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85994	WGS84	BB93748	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.712	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93748_Mgig_Bot2012_COI	MC47	1	BB93748_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93748	Sample	BB93748	targeted	Present	Sample	Mgig_Bot2012_COI	MC47	COI	45	BB93748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC47	TCTTATTCGTTGGAGACTTTATAACCCT	144.91173
BB93749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86107	WGS84	BB93749	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93749_Cmae_Bot2011_COI	MC46	1	BB93749_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93749	Sample	BB93749	targeted	Absent	Sample	Cmae_Bot2011_COI	MC46	COI	45	BB93749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC46	ATGAACAGTCTATCCTCCTTTAG	144.91072
BB93749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86107	WGS84	BB93749	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93749_Msal_Bot2012_COI	MC46	1	BB93749_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93749	Sample	BB93749	targeted	Absent	Sample	Msal_Bot2012_COI	MC46	COI	45	BB93749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC46	GYTAGTTCCRATGATGTTAGCTG	144.91072
BB93750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86157	WGS84	BB93750	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93750_Aamu_Bax2006_COI	MC44	1	BB93750_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93750	Sample	BB93750	targeted	Absent	Sample	Aamu_Bax2006_COI	MC44	COI	45	BB93750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC44	GCACAACCGGGATCTTTACTTC	144.91759
BB93750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86157	WGS84	BB93750	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93750_Pcan_Bot2011_IGS	MC44	1	BB93750_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93750	Sample	BB93750	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC44	IGS	45	BB93750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC44	CGTAATCCTCAGTACTGGCTA	144.91759
BB93750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86157	WGS84	BB93750	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.983	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93750_Sspa_Oph2007_COI	MC44	1	BB93750_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93750	Sample	BB93750	targeted	Present	Sample	Sspa_Oph2007_COI	MC44	COI	45	BB93750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC44	GAAGGCCTTCCGATGATC	144.91759
BB93750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86157	WGS84	BB93750	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93750_Upin_Bot2011_COI	MC44	1	BB93750_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93750	Sample	BB93750	targeted	Absent	Sample	Upin_Bot2011_COI	MC44	COI	45	BB93750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC44	CTTTAATTACAGCGTTTTTATTGTTGT	144.91759
BB93751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86161	WGS84	BB93751	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.858	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93751_Mgig_Bot2012_COI	MC43	1	BB93751_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93751	Sample	BB93751	targeted	Present	Sample	Mgig_Bot2012_COI	MC43	COI	45	BB93751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC43	TCTTATTCGTTGGAGACTTTATAACCCT	144.91536
BB93752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86221	WGS84	BB93752	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93752_Cmae_Bot2011_COI	MC42	1	BB93752_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93752	Sample	BB93752	targeted	Absent	Sample	Cmae_Bot2011_COI	MC42	COI	45	BB93752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC42	ATGAACAGTCTATCCTCCTTTAG	144.91719
BB93752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86221	WGS84	BB93752	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93752_Msal_Bot2012_COI	MC42	1	BB93752_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93752	Sample	BB93752	targeted	Absent	Sample	Msal_Bot2012_COI	MC42	COI	45	BB93752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC42	GYTAGTTCCRATGATGTTAGCTG	144.91719
BB93754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86172	WGS84	BB93754	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93754_Aamu_Bax2006_COI	MC39	1	BB93754_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93754	Sample	BB93754	targeted	Absent	Sample	Aamu_Bax2006_COI	MC39	COI	45	BB93754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC39	GCACAACCGGGATCTTTACTTC	144.91464
BB93754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86172	WGS84	BB93754	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93754_Pcan_Bot2011_IGS	MC39	1	BB93754_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93754	Sample	BB93754	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC39	IGS	45	BB93754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC39	CGTAATCCTCAGTACTGGCTA	144.91464
BB93754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86172	WGS84	BB93754	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.518	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93754_Sspa_Oph2007_COI	MC39	1	BB93754_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93754	Sample	BB93754	targeted	Present	Sample	Sspa_Oph2007_COI	MC39	COI	45	BB93754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC39	GAAGGCCTTCCGATGATC	144.91464
BB93754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86172	WGS84	BB93754	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93754_Upin_Bot2011_COI	MC39	1	BB93754_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93754	Sample	BB93754	targeted	Absent	Sample	Upin_Bot2011_COI	MC39	COI	45	BB93754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC39	CTTTAATTACAGCGTTTTTATTGTTGT	144.91464
BB93755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86339	WGS84	BB93755	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.77	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93755_Mgig_Bot2012_COI	MC38	1	BB93755_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93755	Sample	BB93755	targeted	Present	Sample	Mgig_Bot2012_COI	MC38	COI	45	BB93755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC38	TCTTATTCGTTGGAGACTTTATAACCCT	144.91381
BB93756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86366	WGS84	BB93756	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93756_Cmae_Bot2011_COI	MC37	1	BB93756_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93756	Sample	BB93756	targeted	Absent	Sample	Cmae_Bot2011_COI	MC37	COI	45	BB93756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC37	ATGAACAGTCTATCCTCCTTTAG	144.91806
BB93756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86366	WGS84	BB93756	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93756_Msal_Bot2012_COI	MC37	1	BB93756_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93756	Sample	BB93756	targeted	Absent	Sample	Msal_Bot2012_COI	MC37	COI	45	BB93756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC37	GYTAGTTCCRATGATGTTAGCTG	144.91806
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.665	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93758_Aamu_Bax2006_COI	MC33	1	BB93758_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Present	Sample	Aamu_Bax2006_COI	MC33	COI	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	GCACAACCGGGATCTTTACTTC	144.91624
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93758_Pcan_Bot2011_IGS	MC33	1	BB93758_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC33	IGS	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	CGTAATCCTCAGTACTGGCTA	144.91624
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.02	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93758_Sspa_Oph2007_COI	MC33	1	BB93758_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Present	Sample	Sspa_Oph2007_COI	MC33	COI	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	GAAGGCCTTCCGATGATC	144.91624
BB93758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86425	WGS84	BB93758	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.298	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93758_Upin_Bot2011_COI	MC33	1	BB93758_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93758	Sample	BB93758	targeted	Present	Sample	Upin_Bot2011_COI	MC33	COI	45	BB93758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC33	CTTTAATTACAGCGTTTTTATTGTTGT	144.91624
BB93759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86473	WGS84	BB93759	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.205	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93759_Mgig_Bot2012_COI	MC34	1	BB93759_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93759	Sample	BB93759	targeted	Present	Sample	Mgig_Bot2012_COI	MC34	COI	45	BB93759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC34	TCTTATTCGTTGGAGACTTTATAACCCT	144.91507
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93761_Aamu_Bax2006_COI	MC29	1	BB93761_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Absent	Sample	Aamu_Bax2006_COI	MC29	COI	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	GCACAACCGGGATCTTTACTTC	144.91673
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93761_Pcan_Bot2011_IGS	MC29	1	BB93761_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC29	IGS	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	CGTAATCCTCAGTACTGGCTA	144.91673
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.707	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93761_Sspa_Oph2007_COI	MC29	1	BB93761_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Present	Sample	Sspa_Oph2007_COI	MC29	COI	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	GAAGGCCTTCCGATGATC	144.91673
BB93761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.86396	WGS84	BB93761	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.635	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93761_Upin_Bot2011_COI	MC29	1	BB93761_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93761	Sample	BB93761	targeted	Present	Sample	Upin_Bot2011_COI	MC29	COI	45	BB93761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC29	CTTTAATTACAGCGTTTTTATTGTTGT	144.91673
BB93762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84237	WGS84	BB93762	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.286	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93762_Mgig_Bot2012_COI	MC26	1	BB93762_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93762	Sample	BB93762	targeted	Present	Sample	Mgig_Bot2012_COI	MC26	COI	45	BB93762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC26	TCTTATTCGTTGGAGACTTTATAACCCT	144.90518
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93763_Cmae_Bot2011_COI	MC24	1	BB93763_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Absent	Sample	Cmae_Bot2011_COI	MC24	COI	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	ATGAACAGTCTATCCTCCTTTAG	144.90579
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93763_Hsan_Wil2021_COI	MC24	1	BB93763_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Absent	Sample	Hsan_Wil2021_COI	MC24	COI	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	CCTGGGCCGGTATAGTAGGT	144.90579
BB93763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84402	WGS84	BB93763	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93763_Msal_Bot2012_COI	MC24	1	BB93763_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93763	Sample	BB93763	targeted	Absent	Sample	Msal_Bot2012_COI	MC24	COI	45	BB93763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC24	GYTAGTTCCRATGATGTTAGCTG	144.90579
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93765_Aamu_Bax2006_COI	MC17	1	BB93765_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Absent	Sample	Aamu_Bax2006_COI	MC17	COI	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	GCACAACCGGGATCTTTACTTC	144.91022
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93765_Pcan_Bot2011_IGS	MC17	1	BB93765_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC17	IGS	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	CGTAATCCTCAGTACTGGCTA	144.91022
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.233	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93765_Sspa_Oph2007_COI	MC17	1	BB93765_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Present	Sample	Sspa_Oph2007_COI	MC17	COI	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	GAAGGCCTTCCGATGATC	144.91022
BB93765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84773	WGS84	BB93765	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93765_Upin_Bot2011_COI	MC17	1	BB93765_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93765	Sample	BB93765	targeted	Absent	Sample	Upin_Bot2011_COI	MC17	COI	45	BB93765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC17	CTTTAATTACAGCGTTTTTATTGTTGT	144.91022
BB93766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84987	WGS84	BB93766	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.776	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93766_Mgig_Bot2012_COI	MC16	1	BB93766_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93766	Sample	BB93766	targeted	Present	Sample	Mgig_Bot2012_COI	MC16	COI	45	BB93766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC16	TCTTATTCGTTGGAGACTTTATAACCCT	144.91374
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93767_Cmae_Bot2011_COI	MC13	1	BB93767_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Absent	Sample	Cmae_Bot2011_COI	MC13	COI	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	ATGAACAGTCTATCCTCCTTTAG	144.91209
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93767_Hsan_Wil2021_COI	MC13	1	BB93767_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Absent	Sample	Hsan_Wil2021_COI	MC13	COI	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	CCTGGGCCGGTATAGTAGGT	144.91209
BB93767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84989	WGS84	BB93767	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93767_Msal_Bot2012_COI	MC13	1	BB93767_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93767	Sample	BB93767	targeted	Absent	Sample	Msal_Bot2012_COI	MC13	COI	45	BB93767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC13	GYTAGTTCCRATGATGTTAGCTG	144.91209
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93769_Aamu_Bax2006_COI	MC9	1	BB93769_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Absent	Sample	Aamu_Bax2006_COI	MC9	COI	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	GCACAACCGGGATCTTTACTTC	144.92542
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93769_Pcan_Bot2011_IGS	MC9	1	BB93769_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC9	IGS	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	CGTAATCCTCAGTACTGGCTA	144.92542
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.002	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93769_Sspa_Oph2007_COI	MC9	1	BB93769_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Present	Sample	Sspa_Oph2007_COI	MC9	COI	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	GAAGGCCTTCCGATGATC	144.92542
BB93769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84252	WGS84	BB93769	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93769_Upin_Bot2011_COI	MC9	1	BB93769_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93769	Sample	BB93769	targeted	Absent	Sample	Upin_Bot2011_COI	MC9	COI	45	BB93769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC9	CTTTAATTACAGCGTTTTTATTGTTGT	144.92542
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93770_Cmae_Bot2011_COI	MC98	1	BB93770_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Absent	Sample	Cmae_Bot2011_COI	MC98	COI	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	ATGAACAGTCTATCCTCCTTTAG	144.91328
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93770_Hsan_Wil2021_COI	MC98	1	BB93770_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Absent	Sample	Hsan_Wil2021_COI	MC98	COI	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	CCTGGGCCGGTATAGTAGGT	144.91328
BB93770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81835	WGS84	BB93770	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93770_Msal_Bot2012_COI	MC98	1	BB93770_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93770	Sample	BB93770	targeted	Absent	Sample	Msal_Bot2012_COI	MC98	COI	45	BB93770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC98	GYTAGTTCCRATGATGTTAGCTG	144.91328
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93772_Aamu_Bax2006_COI	MC94	1	BB93772_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Absent	Sample	Aamu_Bax2006_COI	MC94	COI	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	GCACAACCGGGATCTTTACTTC	144.91439
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93772_Pcan_Bot2011_IGS	MC94	1	BB93772_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC94	IGS	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	CGTAATCCTCAGTACTGGCTA	144.91439
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.281	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93772_Sspa_Oph2007_COI	MC94	1	BB93772_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Present	Sample	Sspa_Oph2007_COI	MC94	COI	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	GAAGGCCTTCCGATGATC	144.91439
BB93772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81592	WGS84	BB93772	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93772_Upin_Bot2011_COI	MC94	1	BB93772_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93772	Sample	BB93772	targeted	Absent	Sample	Upin_Bot2011_COI	MC94	COI	45	BB93772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC94	CTTTAATTACAGCGTTTTTATTGTTGT	144.91439
BB93773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81231	WGS84	BB93773	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.361	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93773_Mgig_Bot2012_COI	MC91	1	BB93773_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93773	Sample	BB93773	targeted	Present	Sample	Mgig_Bot2012_COI	MC91	COI	45	BB93773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC91	TCTTATTCGTTGGAGACTTTATAACCCT	144.91333
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93774_Cmae_Bot2011_COI	MC89	1	BB93774_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Absent	Sample	Cmae_Bot2011_COI	MC89	COI	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	ATGAACAGTCTATCCTCCTTTAG	144.91377
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93774_Hsan_Wil2021_COI	MC89	1	BB93774_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Absent	Sample	Hsan_Wil2021_COI	MC89	COI	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	CCTGGGCCGGTATAGTAGGT	144.91377
BB93774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81071	WGS84	BB93774	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93774_Msal_Bot2012_COI	MC89	1	BB93774_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93774	Sample	BB93774	targeted	Absent	Sample	Msal_Bot2012_COI	MC89	COI	45	BB93774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC89	GYTAGTTCCRATGATGTTAGCTG	144.91377
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93776_Aamu_Bax2006_COI	MC87	1	BB93776_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Absent	Sample	Aamu_Bax2006_COI	MC87	COI	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	GCACAACCGGGATCTTTACTTC	144.92133
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93776_Pcan_Bot2011_IGS	MC87	1	BB93776_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC87	IGS	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	CGTAATCCTCAGTACTGGCTA	144.92133
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.126	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93776_Sspa_Oph2007_COI	MC87	1	BB93776_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Present	Sample	Sspa_Oph2007_COI	MC87	COI	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	GAAGGCCTTCCGATGATC	144.92133
BB93776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81952	WGS84	BB93776	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93776_Upin_Bot2011_COI	MC87	1	BB93776_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93776	Sample	BB93776	targeted	Absent	Sample	Upin_Bot2011_COI	MC87	COI	45	BB93776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC87	CTTTAATTACAGCGTTTTTATTGTTGT	144.92133
BB93777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81702	WGS84	BB93777	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.897	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93777_Mgig_Bot2012_COI	MC82	1	BB93777_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93777	Sample	BB93777	targeted	Present	Sample	Mgig_Bot2012_COI	MC82	COI	45	BB93777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC82	TCTTATTCGTTGGAGACTTTATAACCCT	144.92242
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93778_Cmae_Bot2011_COI	MC81	1	BB93778_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Absent	Sample	Cmae_Bot2011_COI	MC81	COI	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	ATGAACAGTCTATCCTCCTTTAG	144.92449
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93778_Hsan_Wil2021_COI	MC81	1	BB93778_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Absent	Sample	Hsan_Wil2021_COI	MC81	COI	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	CCTGGGCCGGTATAGTAGGT	144.92449
BB93778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81787	WGS84	BB93778	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93778_Msal_Bot2012_COI	MC81	1	BB93778_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93778	Sample	BB93778	targeted	Absent	Sample	Msal_Bot2012_COI	MC81	COI	45	BB93778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC81	GYTAGTTCCRATGATGTTAGCTG	144.92449
BB93780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84373	WGS84	BB93780	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.135	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93780_Mgig_Bot2012_COI	MC4	1	BB93780_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93780	Sample	BB93780	targeted	Present	Sample	Mgig_Bot2012_COI	MC4	COI	45	BB93780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC4	TCTTATTCGTTGGAGACTTTATAACCCT	144.93289
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93781_Cmae_Bot2011_COI	MC2	1	BB93781_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Absent	Sample	Cmae_Bot2011_COI	MC2	COI	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	ATGAACAGTCTATCCTCCTTTAG	144.93023
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	34.7040825	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93781_Hsan_Wil2021_COI	MC2	1	BB93781_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Present	Sample	Hsan_Wil2021_COI	MC2	COI	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	CCTGGGCCGGTATAGTAGGT	144.93023
BB93781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.84485	WGS84	BB93781	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93781_Msal_Bot2012_COI	MC2	1	BB93781_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93781	Sample	BB93781	targeted	Absent	Sample	Msal_Bot2012_COI	MC2	COI	45	BB93781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC2	GYTAGTTCCRATGATGTTAGCTG	144.93023
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93783_Aamu_Bax2006_COI	MC114	1	BB93783_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Absent	Sample	Aamu_Bax2006_COI	MC114	COI	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	GCACAACCGGGATCTTTACTTC	144.90928
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93783_Pcan_Bot2011_IGS	MC114	1	BB93783_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC114	IGS	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	CGTAATCCTCAGTACTGGCTA	144.90928
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	28.803	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93783_Sspa_Oph2007_COI	MC114	1	BB93783_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Present	Sample	Sspa_Oph2007_COI	MC114	COI	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	GAAGGCCTTCCGATGATC	144.90928
BB93783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82179	WGS84	BB93783	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93783_Upin_Bot2011_COI	MC114	1	BB93783_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93783	Sample	BB93783	targeted	Absent	Sample	Upin_Bot2011_COI	MC114	COI	45	BB93783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC114	CTTTAATTACAGCGTTTTTATTGTTGT	144.90928
BB93784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81988	WGS84	BB93784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.517	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93784_Mgig_Bot2012_COI	MC108	1	BB93784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93784	Sample	BB93784	targeted	Present	Sample	Mgig_Bot2012_COI	MC108	COI	45	BB93784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC108	TCTTATTCGTTGGAGACTTTATAACCCT	144.91152
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93785_Cmae_Bot2011_COI	MC107	1	BB93785_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Absent	Sample	Cmae_Bot2011_COI	MC107	COI	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	ATGAACAGTCTATCCTCCTTTAG	144.90595
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93785_Hsan_Wil2021_COI	MC107	1	BB93785_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Absent	Sample	Hsan_Wil2021_COI	MC107	COI	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	CCTGGGCCGGTATAGTAGGT	144.90595
BB93785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81671	WGS84	BB93785	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93785_Msal_Bot2012_COI	MC107	1	BB93785_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93785	Sample	BB93785	targeted	Absent	Sample	Msal_Bot2012_COI	MC107	COI	45	BB93785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC107	GYTAGTTCCRATGATGTTAGCTG	144.90595
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93787_Aamu_Bax2006_COI	MC104	1	BB93787_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Absent	Sample	Aamu_Bax2006_COI	MC104	COI	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	GCACAACCGGGATCTTTACTTC	144.91268
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93787_Pcan_Bot2011_IGS	MC104	1	BB93787_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC104	IGS	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	CGTAATCCTCAGTACTGGCTA	144.91268
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.541	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93787_Sspa_Oph2007_COI	MC104	1	BB93787_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Present	Sample	Sspa_Oph2007_COI	MC104	COI	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	GAAGGCCTTCCGATGATC	144.91268
BB93787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81912	WGS84	BB93787	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93787_Upin_Bot2011_COI	MC104	1	BB93787_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93787	Sample	BB93787	targeted	Absent	Sample	Upin_Bot2011_COI	MC104	COI	45	BB93787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC104	CTTTAATTACAGCGTTTTTATTGTTGT	144.91268
BB93788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82018	WGS84	BB93788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.783	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93788_Mgig_Bot2012_COI	MC102	1	BB93788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93788	Sample	BB93788	targeted	Present	Sample	Mgig_Bot2012_COI	MC102	COI	45	BB93788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC102	TCTTATTCGTTGGAGACTTTATAACCCT	144.91416
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93789_Cmae_Bot2011_COI	MC101	1	BB93789_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Absent	Sample	Cmae_Bot2011_COI	MC101	COI	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	ATGAACAGTCTATCCTCCTTTAG	144.91999
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BB93789_Hsan_Wil2021_COI	MC101	1	BB93789_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Absent	Sample	Hsan_Wil2021_COI	MC101	COI	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	CCTGGGCCGGTATAGTAGGT	144.91999
BB93789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81922	WGS84	BB93789	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93789_Msal_Bot2012_COI	MC101	1	BB93789_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93789	Sample	BB93789	targeted	Absent	Sample	Msal_Bot2012_COI	MC101	COI	45	BB93789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC101	GYTAGTTCCRATGATGTTAGCTG	144.91999
BB93790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81652	WGS84	BB93790	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.416	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93790_Aamu_Bax2006_COI	MC99	1	BB93790_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93790	Sample	BB93790	targeted	Present	Sample	Aamu_Bax2006_COI	MC99	COI	45	BB93790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC99	GCACAACCGGGATCTTTACTTC	144.91313
BB93790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81652	WGS84	BB93790	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93790_Pcan_Bot2011_IGS	MC99	1	BB93790_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93790	Sample	BB93790	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC99	IGS	45	BB93790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC99	CGTAATCCTCAGTACTGGCTA	144.91313
BB93790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81652	WGS84	BB93790	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.791	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93790_Sspa_Oph2007_COI	MC99	1	BB93790_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93790	Sample	BB93790	targeted	Present	Sample	Sspa_Oph2007_COI	MC99	COI	45	BB93790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC99	GAAGGCCTTCCGATGATC	144.91313
BB93790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.81652	WGS84	BB93790	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93790_Upin_Bot2011_COI	MC99	1	BB93790_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93790	Sample	BB93790	targeted	Absent	Sample	Upin_Bot2011_COI	MC99	COI	45	BB93790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC99	CTTTAATTACAGCGTTTTTATTGTTGT	144.91313
BB93791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85956	WGS84	BB93791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.703	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93791_Mgig_Bot2012_COI	MC126	1	BB93791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93791	Sample	BB93791	targeted	Present	Sample	Mgig_Bot2012_COI	MC126	COI	45	BB93791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC126	TCTTATTCGTTGGAGACTTTATAACCCT	144.91138
BB93792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85883	WGS84	BB93792	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93792_Cmae_Bot2011_COI	MC124	1	BB93792_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93792	Sample	BB93792	targeted	Absent	Sample	Cmae_Bot2011_COI	MC124	COI	45	BB93792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC124	ATGAACAGTCTATCCTCCTTTAG	144.90837
BB93792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.85883	WGS84	BB93792	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93792_Msal_Bot2012_COI	MC124	1	BB93792_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93792	Sample	BB93792	targeted	Absent	Sample	Msal_Bot2012_COI	MC124	COI	45	BB93792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC124	GYTAGTTCCRATGATGTTAGCTG	144.90837
BB93794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82012	WGS84	BB93794	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.582	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93794_Aamu_Bax2006_COI	MC119	1	BB93794_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93794	Sample	BB93794	targeted	Present	Sample	Aamu_Bax2006_COI	MC119	COI	45	BB93794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC119	GCACAACCGGGATCTTTACTTC	144.90604
BB93794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82012	WGS84	BB93794	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93794_Pcan_Bot2011_IGS	MC119	1	BB93794_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93794	Sample	BB93794	targeted	Absent	Sample	Pcan_Bot2011_IGS	MC119	IGS	45	BB93794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC119	CGTAATCCTCAGTACTGGCTA	144.90604
BB93794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82012	WGS84	BB93794	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.355	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93794_Sspa_Oph2007_COI	MC119	1	BB93794_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93794	Sample	BB93794	targeted	Present	Sample	Sspa_Oph2007_COI	MC119	COI	45	BB93794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC119	GAAGGCCTTCCGATGATC	144.90604
BB93794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82012	WGS84	BB93794	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93794_Upin_Bot2011_COI	MC119	1	BB93794_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93794	Sample	BB93794	targeted	Absent	Sample	Upin_Bot2011_COI	MC119	COI	45	BB93794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC119	CTTTAATTACAGCGTTTTTATTGTTGT	144.90604
BB93795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82075	WGS84	BB93795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.297	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93795_Mgig_Bot2012_COI	MC118	1	BB93795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93795	Sample	BB93795	targeted	Present	Sample	Mgig_Bot2012_COI	MC118	COI	45	BB93795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC118	TCTTATTCGTTGGAGACTTTATAACCCT	144.90629
BB93796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82232	WGS84	BB93796	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93796_Cmae_Bot2011_COI	MC117	1	BB93796_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93796	Sample	BB93796	targeted	Absent	Sample	Cmae_Bot2011_COI	MC117	COI	45	BB93796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC117	ATGAACAGTCTATCCTCCTTTAG	144.90786
BB93796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10455	-37.82232	WGS84	BB93796	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93796_Msal_Bot2012_COI	MC117	1	BB93796_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93796	Sample	BB93796	targeted	Absent	Sample	Msal_Bot2012_COI	MC117	COI	45	BB93796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MC117	GYTAGTTCCRATGATGTTAGCTG	144.90786
BB93802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4292	WGS84	BB93802	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93802_Aamu_Bax2006_COI	BC45	1	BB93802_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93802	Sample	BB93802	targeted	Absent	Sample	Aamu_Bax2006_COI	BC45	COI	45	BB93802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC45	GCACAACCGGGATCTTTACTTC	153.12194
BB93802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4292	WGS84	BB93802	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93802_Pcan_Bot2011_IGS	BC45	1	BB93802_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93802	Sample	BB93802	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC45	IGS	45	BB93802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC45	CGTAATCCTCAGTACTGGCTA	153.12194
BB93802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4292	WGS84	BB93802	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93802_Sspa_Oph2007_COI	BC45	1	BB93802_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93802	Sample	BB93802	targeted	Absent	Sample	Sspa_Oph2007_COI	BC45	COI	45	BB93802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC45	GAAGGCCTTCCGATGATC	153.12194
BB93802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4292	WGS84	BB93802	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93802_Upin_Bot2011_COI	BC45	1	BB93802_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93802	Sample	BB93802	targeted	Absent	Sample	Upin_Bot2011_COI	BC45	COI	45	BB93802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC45	CTTTAATTACAGCGTTTTTATTGTTGT	153.12194
BB93803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4271	WGS84	BB93803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93803_Mgig_Bot2012_COI	BC40	1	BB93803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93803	Sample	BB93803	targeted	Absent	Sample	Mgig_Bot2012_COI	BC40	COI	45	BB93803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC40	TCTTATTCGTTGGAGACTTTATAACCCT	153.1238
BB93804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42155	WGS84	BB93804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93804_Cmae_Bot2011_COI	BC35	1	BB93804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93804	Sample	BB93804	targeted	Absent	Sample	Cmae_Bot2011_COI	BC35	COI	45	BB93804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC35	ATGAACAGTCTATCCTCCTTTAG	153.13604
BB93804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42155	WGS84	BB93804	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93804_Msal_Bot2012_COI	BC35	1	BB93804_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93804	Sample	BB93804	targeted	Absent	Sample	Msal_Bot2012_COI	BC35	COI	45	BB93804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC35	GYTAGTTCCRATGATGTTAGCTG	153.13604
BB93806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44408	WGS84	BB93806	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93806_Aamu_Bax2006_COI	BC55	1	BB93806_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93806	Sample	BB93806	targeted	Absent	Sample	Aamu_Bax2006_COI	BC55	COI	45	BB93806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC55	GCACAACCGGGATCTTTACTTC	153.07674
BB93806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44408	WGS84	BB93806	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93806_Pcan_Bot2011_IGS	BC55	1	BB93806_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93806	Sample	BB93806	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC55	IGS	45	BB93806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC55	CGTAATCCTCAGTACTGGCTA	153.07674
BB93806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44408	WGS84	BB93806	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93806_Sspa_Oph2007_COI	BC55	1	BB93806_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93806	Sample	BB93806	targeted	Absent	Sample	Sspa_Oph2007_COI	BC55	COI	45	BB93806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC55	GAAGGCCTTCCGATGATC	153.07674
BB93806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44408	WGS84	BB93806	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93806_Upin_Bot2011_COI	BC55	1	BB93806_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93806	Sample	BB93806	targeted	Absent	Sample	Upin_Bot2011_COI	BC55	COI	45	BB93806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC55	CTTTAATTACAGCGTTTTTATTGTTGT	153.07674
BB93807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3627	WGS84	BB93807	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93807_Mgig_Bot2012_COI	BC5	1	BB93807_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93807	Sample	BB93807	targeted	Absent	Sample	Mgig_Bot2012_COI	BC5	COI	45	BB93807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC5	TCTTATTCGTTGGAGACTTTATAACCCT	153.17652
BB93808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38487	WGS84	BB93808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93808_Cmae_Bot2011_COI	BC20	1	BB93808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93808	Sample	BB93808	targeted	Absent	Sample	Cmae_Bot2011_COI	BC20	COI	45	BB93808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC20	ATGAACAGTCTATCCTCCTTTAG	153.15971
BB93808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38487	WGS84	BB93808	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93808_Msal_Bot2012_COI	BC20	1	BB93808_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93808	Sample	BB93808	targeted	Absent	Sample	Msal_Bot2012_COI	BC20	COI	45	BB93808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC20	GYTAGTTCCRATGATGTTAGCTG	153.15971
BB93810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44751	WGS84	BB93810	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93810_Mgig_Bot2012_COI	BC50	1	BB93810_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93810	Sample	BB93810	targeted	Absent	Sample	Mgig_Bot2012_COI	BC50	COI	45	BB93810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC50	TCTTATTCGTTGGAGACTTTATAACCCT	153.09566
BB93811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43258	WGS84	BB93811	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93811_Cmae_Bot2011_COI	BC48	1	BB93811_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93811	Sample	BB93811	targeted	Absent	Sample	Cmae_Bot2011_COI	BC48	COI	45	BB93811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC48	ATGAACAGTCTATCCTCCTTTAG	153.11706
BB93811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.43258	WGS84	BB93811	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93811_Msal_Bot2012_COI	BC48	1	BB93811_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93811	Sample	BB93811	targeted	Absent	Sample	Msal_Bot2012_COI	BC48	COI	45	BB93811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC48	GYTAGTTCCRATGATGTTAGCTG	153.11706
BB93813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42924	WGS84	BB93813	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93813_Aamu_Bax2006_COI	BC41	1	BB93813_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93813	Sample	BB93813	targeted	Absent	Sample	Aamu_Bax2006_COI	BC41	COI	45	BB93813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC41	GCACAACCGGGATCTTTACTTC	153.1243
BB93813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42924	WGS84	BB93813	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93813_Pcan_Bot2011_IGS	BC41	1	BB93813_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93813	Sample	BB93813	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC41	IGS	45	BB93813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC41	CGTAATCCTCAGTACTGGCTA	153.1243
BB93813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42924	WGS84	BB93813	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93813_Sspa_Oph2007_COI	BC41	1	BB93813_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93813	Sample	BB93813	targeted	Absent	Sample	Sspa_Oph2007_COI	BC41	COI	45	BB93813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC41	GAAGGCCTTCCGATGATC	153.1243
BB93813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42924	WGS84	BB93813	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93813_Upin_Bot2011_COI	BC41	1	BB93813_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93813	Sample	BB93813	targeted	Absent	Sample	Upin_Bot2011_COI	BC41	COI	45	BB93813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC41	CTTTAATTACAGCGTTTTTATTGTTGT	153.1243
BB93814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.42466	WGS84	BB93814	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93814_Mgig_Bot2012_COI	BC38	1	BB93814_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93814	Sample	BB93814	targeted	Absent	Sample	Mgig_Bot2012_COI	BC38	COI	45	BB93814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC38	TCTTATTCGTTGGAGACTTTATAACCCT	153.12943
BB93815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.41408	WGS84	BB93815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93815_Cmae_Bot2011_COI	BC34	1	BB93815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93815	Sample	BB93815	targeted	Absent	Sample	Cmae_Bot2011_COI	BC34	COI	45	BB93815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC34	ATGAACAGTCTATCCTCCTTTAG	153.14362
BB93815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.41408	WGS84	BB93815	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93815_Msal_Bot2012_COI	BC34	1	BB93815_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93815	Sample	BB93815	targeted	Absent	Sample	Msal_Bot2012_COI	BC34	COI	45	BB93815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC34	GYTAGTTCCRATGATGTTAGCTG	153.14362
BB93817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4041	WGS84	BB93817	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93817_Aamu_Bax2006_COI	BC31	1	BB93817_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93817	Sample	BB93817	targeted	Absent	Sample	Aamu_Bax2006_COI	BC31	COI	45	BB93817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC31	GCACAACCGGGATCTTTACTTC	153.15312
BB93817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4041	WGS84	BB93817	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93817_Pcan_Bot2011_IGS	BC31	1	BB93817_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93817	Sample	BB93817	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC31	IGS	45	BB93817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC31	CGTAATCCTCAGTACTGGCTA	153.15312
BB93817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4041	WGS84	BB93817	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93817_Sspa_Oph2007_COI	BC31	1	BB93817_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93817	Sample	BB93817	targeted	Absent	Sample	Sspa_Oph2007_COI	BC31	COI	45	BB93817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC31	GAAGGCCTTCCGATGATC	153.15312
BB93817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.4041	WGS84	BB93817	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93817_Upin_Bot2011_COI	BC31	1	BB93817_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93817	Sample	BB93817	targeted	Absent	Sample	Upin_Bot2011_COI	BC31	COI	45	BB93817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC31	CTTTAATTACAGCGTTTTTATTGTTGT	153.15312
BB93818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40084	WGS84	BB93818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93818_Mgig_Bot2012_COI	BC29	1	BB93818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93818	Sample	BB93818	targeted	Absent	Sample	Mgig_Bot2012_COI	BC29	COI	45	BB93818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC29	TCTTATTCGTTGGAGACTTTATAACCCT	153.15682
BB93819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40095	WGS84	BB93819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93819_Cmae_Bot2011_COI	BC28	1	BB93819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93819	Sample	BB93819	targeted	Absent	Sample	Cmae_Bot2011_COI	BC28	COI	45	BB93819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC28	ATGAACAGTCTATCCTCCTTTAG	153.1607
BB93819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40095	WGS84	BB93819	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93819_Msal_Bot2012_COI	BC28	1	BB93819_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93819	Sample	BB93819	targeted	Absent	Sample	Msal_Bot2012_COI	BC28	COI	45	BB93819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC28	GYTAGTTCCRATGATGTTAGCTG	153.1607
BB93820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40096	WGS84	BB93820	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93820_Aamu_Bax2006_COI	BC26	1	BB93820_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93820	Sample	BB93820	targeted	Absent	Sample	Aamu_Bax2006_COI	BC26	COI	45	BB93820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC26	GCACAACCGGGATCTTTACTTC	153.16291
BB93820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40096	WGS84	BB93820	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93820_Pcan_Bot2011_IGS	BC26	1	BB93820_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93820	Sample	BB93820	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC26	IGS	45	BB93820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC26	CGTAATCCTCAGTACTGGCTA	153.16291
BB93820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40096	WGS84	BB93820	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93820_Sspa_Oph2007_COI	BC26	1	BB93820_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93820	Sample	BB93820	targeted	Absent	Sample	Sspa_Oph2007_COI	BC26	COI	45	BB93820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC26	GAAGGCCTTCCGATGATC	153.16291
BB93820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.40096	WGS84	BB93820	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93820_Upin_Bot2011_COI	BC26	1	BB93820_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93820	Sample	BB93820	targeted	Absent	Sample	Upin_Bot2011_COI	BC26	COI	45	BB93820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC26	CTTTAATTACAGCGTTTTTATTGTTGT	153.16291
BB93821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44624	WGS84	BB93821	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93821_Mgig_Bot2012_COI	BC62	1	BB93821_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93821	Sample	BB93821	targeted	Absent	Sample	Mgig_Bot2012_COI	BC62	COI	45	BB93821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC62	TCTTATTCGTTGGAGACTTTATAACCCT	153.07816
BB93822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44499	WGS84	BB93822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93822_Cmae_Bot2011_COI	BC61	1	BB93822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93822	Sample	BB93822	targeted	Absent	Sample	Cmae_Bot2011_COI	BC61	COI	45	BB93822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC61	ATGAACAGTCTATCCTCCTTTAG	153.08037
BB93822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44499	WGS84	BB93822	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93822_Msal_Bot2012_COI	BC61	1	BB93822_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93822	Sample	BB93822	targeted	Absent	Sample	Msal_Bot2012_COI	BC61	COI	45	BB93822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC61	GYTAGTTCCRATGATGTTAGCTG	153.08037
BB93824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44527	WGS84	BB93824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93824_Aamu_Bax2006_COI	BC56	1	BB93824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93824	Sample	BB93824	targeted	Absent	Sample	Aamu_Bax2006_COI	BC56	COI	45	BB93824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC56	GCACAACCGGGATCTTTACTTC	153.07559
BB93824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44527	WGS84	BB93824	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93824_Pcan_Bot2011_IGS	BC56	1	BB93824_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93824	Sample	BB93824	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC56	IGS	45	BB93824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC56	CGTAATCCTCAGTACTGGCTA	153.07559
BB93824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44527	WGS84	BB93824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93824_Sspa_Oph2007_COI	BC56	1	BB93824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93824	Sample	BB93824	targeted	Absent	Sample	Sspa_Oph2007_COI	BC56	COI	45	BB93824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC56	GAAGGCCTTCCGATGATC	153.07559
BB93824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44527	WGS84	BB93824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93824_Upin_Bot2011_COI	BC56	1	BB93824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93824	Sample	BB93824	targeted	Absent	Sample	Upin_Bot2011_COI	BC56	COI	45	BB93824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC56	CTTTAATTACAGCGTTTTTATTGTTGT	153.07559
BB93825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44414	WGS84	BB93825	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93825_Mgig_Bot2012_COI	BC54	1	BB93825_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93825	Sample	BB93825	targeted	Absent	Sample	Mgig_Bot2012_COI	BC54	COI	45	BB93825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC54	TCTTATTCGTTGGAGACTTTATAACCCT	153.07558
BB93826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44257	WGS84	BB93826	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93826_Cmae_Bot2011_COI	BC52	1	BB93826_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93826	Sample	BB93826	targeted	Absent	Sample	Cmae_Bot2011_COI	BC52	COI	45	BB93826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC52	ATGAACAGTCTATCCTCCTTTAG	153.07162
BB93826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.44257	WGS84	BB93826	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/04/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93826_Msal_Bot2012_COI	BC52	1	BB93826_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93826	Sample	BB93826	targeted	Absent	Sample	Msal_Bot2012_COI	BC52	COI	45	BB93826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC52	GYTAGTTCCRATGATGTTAGCTG	153.07162
BB93828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36653	WGS84	BB93828	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93828_Aamu_Bax2006_COI	BC7	1	BB93828_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93828	Sample	BB93828	targeted	Absent	Sample	Aamu_Bax2006_COI	BC7	COI	45	BB93828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC7	GCACAACCGGGATCTTTACTTC	153.17384
BB93828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36653	WGS84	BB93828	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93828_Pcan_Bot2011_IGS	BC7	1	BB93828_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93828	Sample	BB93828	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC7	IGS	45	BB93828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC7	CGTAATCCTCAGTACTGGCTA	153.17384
BB93828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36653	WGS84	BB93828	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93828_Sspa_Oph2007_COI	BC7	1	BB93828_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93828	Sample	BB93828	targeted	Absent	Sample	Sspa_Oph2007_COI	BC7	COI	45	BB93828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC7	GAAGGCCTTCCGATGATC	153.17384
BB93828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36653	WGS84	BB93828	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93828_Upin_Bot2011_COI	BC7	1	BB93828_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93828	Sample	BB93828	targeted	Absent	Sample	Upin_Bot2011_COI	BC7	COI	45	BB93828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC7	CTTTAATTACAGCGTTTTTATTGTTGT	153.17384
BB93829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.36126	WGS84	BB93829	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93829_Mgig_Bot2012_COI	BC4	1	BB93829_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93829	Sample	BB93829	targeted	Absent	Sample	Mgig_Bot2012_COI	BC4	COI	45	BB93829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC4	TCTTATTCGTTGGAGACTTTATAACCCT	153.17768
BB93831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3909	WGS84	BB93831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93831_Aamu_Bax2006_COI	BC24	1	BB93831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93831	Sample	BB93831	targeted	Absent	Sample	Aamu_Bax2006_COI	BC24	COI	45	BB93831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC24	GCACAACCGGGATCTTTACTTC	153.15723
BB93831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3909	WGS84	BB93831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93831_Pcan_Bot2011_IGS	BC24	1	BB93831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93831	Sample	BB93831	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC24	IGS	45	BB93831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC24	CGTAATCCTCAGTACTGGCTA	153.15723
BB93831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3909	WGS84	BB93831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93831_Sspa_Oph2007_COI	BC24	1	BB93831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93831	Sample	BB93831	targeted	Absent	Sample	Sspa_Oph2007_COI	BC24	COI	45	BB93831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC24	GAAGGCCTTCCGATGATC	153.15723
BB93831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.3909	WGS84	BB93831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93831_Upin_Bot2011_COI	BC24	1	BB93831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93831	Sample	BB93831	targeted	Absent	Sample	Upin_Bot2011_COI	BC24	COI	45	BB93831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC24	CTTTAATTACAGCGTTTTTATTGTTGT	153.15723
BB93832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38726	WGS84	BB93832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93832_Mgig_Bot2012_COI	BC22	1	BB93832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93832	Sample	BB93832	targeted	Absent	Sample	Mgig_Bot2012_COI	BC22	COI	45	BB93832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC22	TCTTATTCGTTGGAGACTTTATAACCCT	153.15893
BB93833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38259	WGS84	BB93833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93833_Cmae_Bot2011_COI	BC18	1	BB93833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93833	Sample	BB93833	targeted	Absent	Sample	Cmae_Bot2011_COI	BC18	COI	45	BB93833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC18	ATGAACAGTCTATCCTCCTTTAG	153.16112
BB93833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.38259	WGS84	BB93833	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93833_Msal_Bot2012_COI	BC18	1	BB93833_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93833	Sample	BB93833	targeted	Absent	Sample	Msal_Bot2012_COI	BC18	COI	45	BB93833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC18	GYTAGTTCCRATGATGTTAGCTG	153.16112
BB93835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37563	WGS84	BB93835	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93835_Aamu_Bax2006_COI	BC13	1	BB93835_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93835	Sample	BB93835	targeted	Absent	Sample	Aamu_Bax2006_COI	BC13	COI	45	BB93835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC13	GCACAACCGGGATCTTTACTTC	153.16734
BB93835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37563	WGS84	BB93835	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93835_Pcan_Bot2011_IGS	BC13	1	BB93835_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93835	Sample	BB93835	targeted	Absent	Sample	Pcan_Bot2011_IGS	BC13	IGS	45	BB93835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC13	CGTAATCCTCAGTACTGGCTA	153.16734
BB93835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37563	WGS84	BB93835	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93835_Sspa_Oph2007_COI	BC13	1	BB93835_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93835	Sample	BB93835	targeted	Absent	Sample	Sspa_Oph2007_COI	BC13	COI	45	BB93835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC13	GAAGGCCTTCCGATGATC	153.16734
BB93835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37563	WGS84	BB93835	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93835_Upin_Bot2011_COI	BC13	1	BB93835_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93835	Sample	BB93835	targeted	Absent	Sample	Upin_Bot2011_COI	BC13	COI	45	BB93835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC13	CTTTAATTACAGCGTTTTTATTGTTGT	153.16734
BB93836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.37255	WGS84	BB93836	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93836_Mgig_Bot2012_COI	BC11	1	BB93836_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93836	Sample	BB93836	targeted	Absent	Sample	Mgig_Bot2012_COI	BC11	COI	45	BB93836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC11	TCTTATTCGTTGGAGACTTTATAACCCT	153.16947
BB93837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35649	WGS84	BB93837	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93837_Cmae_Bot2011_COI	BC1	1	BB93837_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93837	Sample	BB93837	targeted	Absent	Sample	Cmae_Bot2011_COI	BC1	COI	45	BB93837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC1	ATGAACAGTCTATCCTCCTTTAG	153.18075
BB93837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10454	-27.35649	WGS84	BB93837	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Brisbane_Summer	4/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93837_Msal_Bot2012_COI	BC1	1	BB93837_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93837	Sample	BB93837	targeted	Absent	Sample	Msal_Bot2012_COI	BC1	COI	45	BB93837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BC1	GYTAGTTCCRATGATGTTAGCTG	153.18075
BB93854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8765	WGS84	BB93854	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.324	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93854_Mgig_Bot2012_COI	HC20	1	BB93854_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93854	Sample	BB93854	targeted	Present	Sample	Mgig_Bot2012_COI	HC20	COI	45	BB93854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC20	TCTTATTCGTTGGAGACTTTATAACCCT	147.3401
BB93855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.844	WGS84	BB93855	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93855_Cmae_Bot2011_COI	HC5	1	BB93855_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93855	Sample	BB93855	targeted	Absent	Sample	Cmae_Bot2011_COI	HC5	COI	45	BB93855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC5	ATGAACAGTCTATCCTCCTTTAG	147.3277
BB93855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.844	WGS84	BB93855	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93855_Msal_Bot2012_COI	HC5	1	BB93855_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93855	Sample	BB93855	targeted	Absent	Sample	Msal_Bot2012_COI	HC5	COI	45	BB93855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC5	GYTAGTTCCRATGATGTTAGCTG	147.3277
BB93857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.875	WGS84	BB93857	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93857_Aamu_Bax2006_COI	HC22	1	BB93857_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93857	Sample	BB93857	targeted	Absent	Sample	Aamu_Bax2006_COI	HC22	COI	45	BB93857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC22	GCACAACCGGGATCTTTACTTC	147.3394
BB93857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.875	WGS84	BB93857	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93857_Pcan_Bot2011_IGS	HC22	1	BB93857_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93857	Sample	BB93857	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC22	IGS	45	BB93857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC22	CGTAATCCTCAGTACTGGCTA	147.3394
BB93857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.875	WGS84	BB93857	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93857_Sspa_Oph2007_COI	HC22	1	BB93857_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93857	Sample	BB93857	targeted	Absent	Sample	Sspa_Oph2007_COI	HC22	COI	45	BB93857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC22	GAAGGCCTTCCGATGATC	147.3394
BB93857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.875	WGS84	BB93857	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93857_Upin_Bot2011_COI	HC22	1	BB93857_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93857	Sample	BB93857	targeted	Absent	Sample	Upin_Bot2011_COI	HC22	COI	45	BB93857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC22	CTTTAATTACAGCGTTTTTATTGTTGT	147.3394
BB93858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8786	WGS84	BB93858	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.553	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93858_Mgig_Bot2012_COI	HC19	1	BB93858_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93858	Sample	BB93858	targeted	Present	Sample	Mgig_Bot2012_COI	HC19	COI	45	BB93858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC19	TCTTATTCGTTGGAGACTTTATAACCCT	147.3421
BB93859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8818	WGS84	BB93859	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.324	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93859_Cmae_Bot2011_COI	HC17	1	BB93859_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93859	Sample	BB93859	targeted	Present	Sample	Cmae_Bot2011_COI	HC17	COI	45	BB93859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC17	ATGAACAGTCTATCCTCCTTTAG	147.3426
BB93859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8818	WGS84	BB93859	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93859_Msal_Bot2012_COI	HC17	1	BB93859_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93859	Sample	BB93859	targeted	Absent	Sample	Msal_Bot2012_COI	HC17	COI	45	BB93859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC17	GYTAGTTCCRATGATGTTAGCTG	147.3426
BB93860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8829	WGS84	BB93860	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93860_Aamu_Bax2006_COI	HC16	1	BB93860_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93860	Sample	BB93860	targeted	Absent	Sample	Aamu_Bax2006_COI	HC16	COI	45	BB93860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC16	GCACAACCGGGATCTTTACTTC	147.3419
BB93860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8829	WGS84	BB93860	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93860_Pcan_Bot2011_IGS	HC16	1	BB93860_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93860	Sample	BB93860	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC16	IGS	45	BB93860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC16	CGTAATCCTCAGTACTGGCTA	147.3419
BB93860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8829	WGS84	BB93860	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93860_Sspa_Oph2007_COI	HC16	1	BB93860_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93860	Sample	BB93860	targeted	Absent	Sample	Sspa_Oph2007_COI	HC16	COI	45	BB93860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC16	GAAGGCCTTCCGATGATC	147.3419
BB93860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8829	WGS84	BB93860	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93860_Upin_Bot2011_COI	HC16	1	BB93860_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93860	Sample	BB93860	targeted	Absent	Sample	Upin_Bot2011_COI	HC16	COI	45	BB93860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC16	CTTTAATTACAGCGTTTTTATTGTTGT	147.3419
BB93861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8859	WGS84	BB93861	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.95	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93861_Mgig_Bot2012_COI	HC12	1	BB93861_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93861	Sample	BB93861	targeted	Present	Sample	Mgig_Bot2012_COI	HC12	COI	45	BB93861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC12	TCTTATTCGTTGGAGACTTTATAACCCT	147.3385
BB93862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8828	WGS84	BB93862	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93862_Cmae_Bot2011_COI	HC13	1	BB93862_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93862	Sample	BB93862	targeted	Absent	Sample	Cmae_Bot2011_COI	HC13	COI	45	BB93862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC13	ATGAACAGTCTATCCTCCTTTAG	147.3363
BB93862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8828	WGS84	BB93862	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93862_Msal_Bot2012_COI	HC13	1	BB93862_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93862	Sample	BB93862	targeted	Absent	Sample	Msal_Bot2012_COI	HC13	COI	45	BB93862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC13	GYTAGTTCCRATGATGTTAGCTG	147.3363
BB93864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8461	WGS84	BB93864	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93864_Aamu_Bax2006_COI	HC9	1	BB93864_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93864	Sample	BB93864	targeted	Absent	Sample	Aamu_Bax2006_COI	HC9	COI	45	BB93864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC9	GCACAACCGGGATCTTTACTTC	147.3297
BB93864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8461	WGS84	BB93864	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93864_Pcan_Bot2011_IGS	HC9	1	BB93864_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93864	Sample	BB93864	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC9	IGS	45	BB93864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC9	CGTAATCCTCAGTACTGGCTA	147.3297
BB93864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8461	WGS84	BB93864	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93864_Sspa_Oph2007_COI	HC9	1	BB93864_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93864	Sample	BB93864	targeted	Absent	Sample	Sspa_Oph2007_COI	HC9	COI	45	BB93864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC9	GAAGGCCTTCCGATGATC	147.3297
BB93864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8461	WGS84	BB93864	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93864_Upin_Bot2011_COI	HC9	1	BB93864_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93864	Sample	BB93864	targeted	Absent	Sample	Upin_Bot2011_COI	HC9	COI	45	BB93864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC9	CTTTAATTACAGCGTTTTTATTGTTGT	147.3297
BB93865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93865	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.379	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93865_Mgig_Bot2012_COI	HC8	1	BB93865_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93865	Sample	BB93865	targeted	Present	Sample	Mgig_Bot2012_COI	HC8	COI	45	BB93865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC8	TCTTATTCGTTGGAGACTTTATAACCCT	147.3242
BB93866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93866	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93866_Cmae_Bot2011_COI	HC4	1	BB93866_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93866	Sample	BB93866	targeted	Absent	Sample	Cmae_Bot2011_COI	HC4	COI	45	BB93866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC4	ATGAACAGTCTATCCTCCTTTAG	147.3295
BB93866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.8443	WGS84	BB93866	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93866_Msal_Bot2012_COI	HC4	1	BB93866_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93866	Sample	BB93866	targeted	Absent	Sample	Msal_Bot2012_COI	HC4	COI	45	BB93866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC4	GYTAGTTCCRATGATGTTAGCTG	147.3295
BB93868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.831	WGS84	BB93868	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93868_Aamu_Bax2006_COI	HC1	1	BB93868_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93868	Sample	BB93868	targeted	Absent	Sample	Aamu_Bax2006_COI	HC1	COI	45	BB93868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC1	GCACAACCGGGATCTTTACTTC	147.3215
BB93868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.831	WGS84	BB93868	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93868_Pcan_Bot2011_IGS	HC1	1	BB93868_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93868	Sample	BB93868	targeted	Absent	Sample	Pcan_Bot2011_IGS	HC1	IGS	45	BB93868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC1	CGTAATCCTCAGTACTGGCTA	147.3215
BB93868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.831	WGS84	BB93868	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93868_Sspa_Oph2007_COI	HC1	1	BB93868_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93868	Sample	BB93868	targeted	Absent	Sample	Sspa_Oph2007_COI	HC1	COI	45	BB93868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC1	GAAGGCCTTCCGATGATC	147.3215
BB93868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10456	-42.831	WGS84	BB93868	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93868_Upin_Bot2011_COI	HC1	1	BB93868_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93868	Sample	BB93868	targeted	Absent	Sample	Upin_Bot2011_COI	HC1	COI	45	BB93868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HC1	CTTTAATTACAGCGTTTTTATTGTTGT	147.3215
BB93875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80585	WGS84	BB93875	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93875_Aamu_Bax2006_COI	GC105	1	BB93875_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93875	Sample	BB93875	targeted	Absent	Sample	Aamu_Bax2006_COI	GC105	COI	45	BB93875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC105	GCACAACCGGGATCTTTACTTC	151.21913
BB93875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80585	WGS84	BB93875	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93875_Pcan_Bot2011_IGS	GC105	1	BB93875_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93875	Sample	BB93875	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC105	IGS	45	BB93875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC105	CGTAATCCTCAGTACTGGCTA	151.21913
BB93875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80585	WGS84	BB93875	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93875_Sspa_Oph2007_COI	GC105	1	BB93875_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93875	Sample	BB93875	targeted	Absent	Sample	Sspa_Oph2007_COI	GC105	COI	45	BB93875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC105	GAAGGCCTTCCGATGATC	151.21913
BB93875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80585	WGS84	BB93875	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93875_Upin_Bot2011_COI	GC105	1	BB93875_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93875	Sample	BB93875	targeted	Absent	Sample	Upin_Bot2011_COI	GC105	COI	45	BB93875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC105	CTTTAATTACAGCGTTTTTATTGTTGT	151.21913
BB93876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85487	WGS84	BB93876	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93876_Mgig_Bot2012_COI	GC10	1	BB93876_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93876	Sample	BB93876	targeted	Absent	Sample	Mgig_Bot2012_COI	GC10	COI	45	BB93876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC10	TCTTATTCGTTGGAGACTTTATAACCCT	151.31994
BB93877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85248	WGS84	BB93877	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93877_Cmae_Bot2011_COI	GC5	1	BB93877_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93877	Sample	BB93877	targeted	Absent	Sample	Cmae_Bot2011_COI	GC5	COI	45	BB93877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC5	ATGAACAGTCTATCCTCCTTTAG	151.31446
BB93877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85248	WGS84	BB93877	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93877_Msal_Bot2012_COI	GC5	1	BB93877_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93877	Sample	BB93877	targeted	Absent	Sample	Msal_Bot2012_COI	GC5	COI	45	BB93877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC5	GYTAGTTCCRATGATGTTAGCTG	151.31446
BB93879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78941	WGS84	BB93879	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93879_Aamu_Bax2006_COI	GC95	1	BB93879_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93879	Sample	BB93879	targeted	Absent	Sample	Aamu_Bax2006_COI	GC95	COI	45	BB93879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC95	GCACAACCGGGATCTTTACTTC	151.18013
BB93879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78941	WGS84	BB93879	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93879_Pcan_Bot2011_IGS	GC95	1	BB93879_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93879	Sample	BB93879	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC95	IGS	45	BB93879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC95	CGTAATCCTCAGTACTGGCTA	151.18013
BB93879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78941	WGS84	BB93879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93879_Sspa_Oph2007_COI	GC95	1	BB93879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93879	Sample	BB93879	targeted	Absent	Sample	Sspa_Oph2007_COI	GC95	COI	45	BB93879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC95	GAAGGCCTTCCGATGATC	151.18013
BB93879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78941	WGS84	BB93879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93879_Upin_Bot2011_COI	GC95	1	BB93879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93879	Sample	BB93879	targeted	Absent	Sample	Upin_Bot2011_COI	GC95	COI	45	BB93879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC95	CTTTAATTACAGCGTTTTTATTGTTGT	151.18013
BB93880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78768	WGS84	BB93880	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93880_Cmae_Bot2011_COI	GC90	1	BB93880_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93880	Sample	BB93880	targeted	Absent	Sample	Cmae_Bot2011_COI	GC90	COI	45	BB93880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC90	ATGAACAGTCTATCCTCCTTTAG	151.17541
BB93880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78768	WGS84	BB93880	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93880_Msal_Bot2012_COI	GC90	1	BB93880_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93880	Sample	BB93880	targeted	Absent	Sample	Msal_Bot2012_COI	GC90	COI	45	BB93880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC90	GYTAGTTCCRATGATGTTAGCTG	151.17541
BB93882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78499	WGS84	BB93882	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93882_Aamu_Bax2006_COI	GC80	1	BB93882_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93882	Sample	BB93882	targeted	Absent	Sample	Aamu_Bax2006_COI	GC80	COI	45	BB93882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC80	GCACAACCGGGATCTTTACTTC	151.20342
BB93882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78499	WGS84	BB93882	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93882_Pcan_Bot2011_IGS	GC80	1	BB93882_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93882	Sample	BB93882	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC80	IGS	45	BB93882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC80	CGTAATCCTCAGTACTGGCTA	151.20342
BB93882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78499	WGS84	BB93882	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93882_Sspa_Oph2007_COI	GC80	1	BB93882_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93882	Sample	BB93882	targeted	Absent	Sample	Sspa_Oph2007_COI	GC80	COI	45	BB93882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC80	GAAGGCCTTCCGATGATC	151.20342
BB93882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78499	WGS84	BB93882	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93882_Upin_Bot2011_COI	GC80	1	BB93882_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93882	Sample	BB93882	targeted	Absent	Sample	Upin_Bot2011_COI	GC80	COI	45	BB93882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC80	CTTTAATTACAGCGTTTTTATTGTTGT	151.20342
BB93883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7791	WGS84	BB93883	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93883_Mgig_Bot2012_COI	GC75	1	BB93883_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93883	Sample	BB93883	targeted	Absent	Sample	Mgig_Bot2012_COI	GC75	COI	45	BB93883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC75	TCTTATTCGTTGGAGACTTTATAACCCT	151.19661
BB93884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81554	WGS84	BB93884	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93884_Cmae_Bot2011_COI	GC65	1	BB93884_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93884	Sample	BB93884	targeted	Absent	Sample	Cmae_Bot2011_COI	GC65	COI	45	BB93884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC65	ATGAACAGTCTATCCTCCTTTAG	151.23926
BB93884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81554	WGS84	BB93884	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93884_Msal_Bot2012_COI	GC65	1	BB93884_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93884	Sample	BB93884	targeted	Absent	Sample	Msal_Bot2012_COI	GC65	COI	45	BB93884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC65	GYTAGTTCCRATGATGTTAGCTG	151.23926
BB93886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8227	WGS84	BB93886	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93886_Aamu_Bax2006_COI	GC55	1	BB93886_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93886	Sample	BB93886	targeted	Absent	Sample	Aamu_Bax2006_COI	GC55	COI	45	BB93886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC55	GCACAACCGGGATCTTTACTTC	151.24297
BB93886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8227	WGS84	BB93886	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93886_Pcan_Bot2011_IGS	GC55	1	BB93886_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93886	Sample	BB93886	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC55	IGS	45	BB93886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC55	CGTAATCCTCAGTACTGGCTA	151.24297
BB93886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8227	WGS84	BB93886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93886_Sspa_Oph2007_COI	GC55	1	BB93886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93886	Sample	BB93886	targeted	Absent	Sample	Sspa_Oph2007_COI	GC55	COI	45	BB93886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC55	GAAGGCCTTCCGATGATC	151.24297
BB93886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8227	WGS84	BB93886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93886_Upin_Bot2011_COI	GC55	1	BB93886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93886	Sample	BB93886	targeted	Absent	Sample	Upin_Bot2011_COI	GC55	COI	45	BB93886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC55	CTTTAATTACAGCGTTTTTATTGTTGT	151.24297
BB93887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82125	WGS84	BB93887	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93887_Mgig_Bot2012_COI	GC50	1	BB93887_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93887	Sample	BB93887	targeted	Absent	Sample	Mgig_Bot2012_COI	GC50	COI	45	BB93887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC50	TCTTATTCGTTGGAGACTTTATAACCCT	151.23987
BB93888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82403	WGS84	BB93888	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93888_Cmae_Bot2011_COI	GC40	1	BB93888_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93888	Sample	BB93888	targeted	Absent	Sample	Cmae_Bot2011_COI	GC40	COI	45	BB93888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC40	ATGAACAGTCTATCCTCCTTTAG	151.2451
BB93888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82403	WGS84	BB93888	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93888_Msal_Bot2012_COI	GC40	1	BB93888_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93888	Sample	BB93888	targeted	Absent	Sample	Msal_Bot2012_COI	GC40	COI	45	BB93888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC40	GYTAGTTCCRATGATGTTAGCTG	151.2451
BB93890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80771	WGS84	BB93890	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93890_Mgig_Bot2012_COI	GC109	1	BB93890_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93890	Sample	BB93890	targeted	Absent	Sample	Mgig_Bot2012_COI	GC109	COI	45	BB93890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC109	TCTTATTCGTTGGAGACTTTATAACCCT	151.21666
BB93891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8081	WGS84	BB93891	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93891_Cmae_Bot2011_COI	GC107	1	BB93891_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93891	Sample	BB93891	targeted	Absent	Sample	Cmae_Bot2011_COI	GC107	COI	45	BB93891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC107	ATGAACAGTCTATCCTCCTTTAG	151.22028
BB93891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8081	WGS84	BB93891	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93891_Msal_Bot2012_COI	GC107	1	BB93891_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93891	Sample	BB93891	targeted	Absent	Sample	Msal_Bot2012_COI	GC107	COI	45	BB93891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC107	GYTAGTTCCRATGATGTTAGCTG	151.22028
BB93893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80814	WGS84	BB93893	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93893_Aamu_Bax2006_COI	GC101	1	BB93893_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93893	Sample	BB93893	targeted	Absent	Sample	Aamu_Bax2006_COI	GC101	COI	45	BB93893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC101	GCACAACCGGGATCTTTACTTC	151.21454
BB93893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80814	WGS84	BB93893	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93893_Pcan_Bot2011_IGS	GC101	1	BB93893_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93893	Sample	BB93893	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC101	IGS	45	BB93893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC101	CGTAATCCTCAGTACTGGCTA	151.21454
BB93893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80814	WGS84	BB93893	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93893_Sspa_Oph2007_COI	GC101	1	BB93893_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93893	Sample	BB93893	targeted	Absent	Sample	Sspa_Oph2007_COI	GC101	COI	45	BB93893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC101	GAAGGCCTTCCGATGATC	151.21454
BB93893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.80814	WGS84	BB93893	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93893_Upin_Bot2011_COI	GC101	1	BB93893_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93893	Sample	BB93893	targeted	Absent	Sample	Upin_Bot2011_COI	GC101	COI	45	BB93893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC101	CTTTAATTACAGCGTTTTTATTGTTGT	151.21454
BB93894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85458	WGS84	BB93894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93894_Mgig_Bot2012_COI	GC12	1	BB93894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93894	Sample	BB93894	targeted	Absent	Sample	Mgig_Bot2012_COI	GC12	COI	45	BB93894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC12	TCTTATTCGTTGGAGACTTTATAACCCT	151.32258
BB93895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85269	WGS84	BB93895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93895_Cmae_Bot2011_COI	GC8	1	BB93895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93895	Sample	BB93895	targeted	Absent	Sample	Cmae_Bot2011_COI	GC8	COI	45	BB93895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC8	ATGAACAGTCTATCCTCCTTTAG	151.31994
BB93895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85269	WGS84	BB93895	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93895_Msal_Bot2012_COI	GC8	1	BB93895_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93895	Sample	BB93895	targeted	Absent	Sample	Msal_Bot2012_COI	GC8	COI	45	BB93895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC8	GYTAGTTCCRATGATGTTAGCTG	151.31994
BB93897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85164	WGS84	BB93897	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93897_Aamu_Bax2006_COI	GC3	1	BB93897_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93897	Sample	BB93897	targeted	Absent	Sample	Aamu_Bax2006_COI	GC3	COI	45	BB93897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC3	GCACAACCGGGATCTTTACTTC	151.3116
BB93897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85164	WGS84	BB93897	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93897_Pcan_Bot2011_IGS	GC3	1	BB93897_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93897	Sample	BB93897	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC3	IGS	45	BB93897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC3	CGTAATCCTCAGTACTGGCTA	151.3116
BB93897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85164	WGS84	BB93897	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93897_Sspa_Oph2007_COI	GC3	1	BB93897_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93897	Sample	BB93897	targeted	Absent	Sample	Sspa_Oph2007_COI	GC3	COI	45	BB93897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC3	GAAGGCCTTCCGATGATC	151.3116
BB93897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85164	WGS84	BB93897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93897_Upin_Bot2011_COI	GC3	1	BB93897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93897	Sample	BB93897	targeted	Absent	Sample	Upin_Bot2011_COI	GC3	COI	45	BB93897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC3	CTTTAATTACAGCGTTTTTATTGTTGT	151.3116
BB93898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.85254	WGS84	BB93898	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93898_Mgig_Bot2012_COI	GC1	1	BB93898_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93898	Sample	BB93898	targeted	Absent	Sample	Mgig_Bot2012_COI	GC1	COI	45	BB93898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC1	TCTTATTCGTTGGAGACTTTATAACCCT	151.30846
BB93899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79153	WGS84	BB93899	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93899_Cmae_Bot2011_COI	GC98	1	BB93899_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93899	Sample	BB93899	targeted	Absent	Sample	Cmae_Bot2011_COI	GC98	COI	45	BB93899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC98	ATGAACAGTCTATCCTCCTTTAG	151.18068
BB93899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79153	WGS84	BB93899	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93899_Msal_Bot2012_COI	GC98	1	BB93899_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93899	Sample	BB93899	targeted	Absent	Sample	Msal_Bot2012_COI	GC98	COI	45	BB93899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC98	GYTAGTTCCRATGATGTTAGCTG	151.18068
BB93901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79014	WGS84	BB93901	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93901_Aamu_Bax2006_COI	GC92	1	BB93901_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93901	Sample	BB93901	targeted	Absent	Sample	Aamu_Bax2006_COI	GC92	COI	45	BB93901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC92	GCACAACCGGGATCTTTACTTC	151.17721
BB93901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79014	WGS84	BB93901	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93901_Pcan_Bot2011_IGS	GC92	1	BB93901_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93901	Sample	BB93901	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC92	IGS	45	BB93901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC92	CGTAATCCTCAGTACTGGCTA	151.17721
BB93901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79014	WGS84	BB93901	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93901_Sspa_Oph2007_COI	GC92	1	BB93901_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93901	Sample	BB93901	targeted	Absent	Sample	Sspa_Oph2007_COI	GC92	COI	45	BB93901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC92	GAAGGCCTTCCGATGATC	151.17721
BB93901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79014	WGS84	BB93901	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93901_Upin_Bot2011_COI	GC92	1	BB93901_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93901	Sample	BB93901	targeted	Absent	Sample	Upin_Bot2011_COI	GC92	COI	45	BB93901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC92	CTTTAATTACAGCGTTTTTATTGTTGT	151.17721
BB93902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78759	WGS84	BB93902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BB93902_Mgig_Bot2012_COI	GC88	1	BB93902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93902	Sample	BB93902	targeted	Absent	Sample	Mgig_Bot2012_COI	GC88	COI	45	BB93902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC88	TCTTATTCGTTGGAGACTTTATAACCCT	151.17442
BB93903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78545	WGS84	BB93903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BB93903_Cmae_Bot2011_COI	GC87	1	BB93903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93903	Sample	BB93903	targeted	Absent	Sample	Cmae_Bot2011_COI	GC87	COI	45	BB93903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC87	ATGAACAGTCTATCCTCCTTTAG	151.17523
BB93903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78545	WGS84	BB93903	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BB93903_Msal_Bot2012_COI	GC87	1	BB93903_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93903	Sample	BB93903	targeted	Absent	Sample	Msal_Bot2012_COI	GC87	COI	45	BB93903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC87	GYTAGTTCCRATGATGTTAGCTG	151.17523
BB93905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7855	WGS84	BB93905	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BB93905_Aamu_Bax2006_COI	GC82	1	BB93905_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93905	Sample	BB93905	targeted	Absent	Sample	Aamu_Bax2006_COI	GC82	COI	45	BB93905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC82	GCACAACCGGGATCTTTACTTC	151.17113
BB93905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7855	WGS84	BB93905	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BB93905_Pcan_Bot2011_IGS	GC82	1	BB93905_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93905	Sample	BB93905	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC82	IGS	45	BB93905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC82	CGTAATCCTCAGTACTGGCTA	151.17113
BB93905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7855	WGS84	BB93905	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BB93905_Sspa_Oph2007_COI	GC82	1	BB93905_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93905	Sample	BB93905	targeted	Absent	Sample	Sspa_Oph2007_COI	GC82	COI	45	BB93905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC82	GAAGGCCTTCCGATGATC	151.17113
BB93905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.7855	WGS84	BB93905	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BB93905_Upin_Bot2011_COI	GC82	1	BB93905_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BB93905	Sample	BB93905	targeted	Absent	Sample	Upin_Bot2011_COI	GC82	COI	45	BB93905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC82	CTTTAATTACAGCGTTTTTATTGTTGT	151.17113
BC05868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8351	WGS84	BC05868	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05868_Aamu_Bax2006_COI	GC16	1	BC05868_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05868	Sample	BC05868	targeted	Absent	Sample	Aamu_Bax2006_COI	GC16	COI	45	BC05868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC16	GCACAACCGGGATCTTTACTTC	151.27295
BC05868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8351	WGS84	BC05868	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05868_Pcan_Bot2011_IGS	GC16	1	BC05868_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05868	Sample	BC05868	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC16	IGS	45	BC05868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC16	CGTAATCCTCAGTACTGGCTA	151.27295
BC05868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8351	WGS84	BC05868	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05868_Sspa_Oph2007_COI	GC16	1	BC05868_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05868	Sample	BC05868	targeted	Absent	Sample	Sspa_Oph2007_COI	GC16	COI	45	BC05868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC16	GAAGGCCTTCCGATGATC	151.27295
BC05868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8351	WGS84	BC05868	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05868_Upin_Bot2011_COI	GC16	1	BC05868_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05868	Sample	BC05868	targeted	Absent	Sample	Upin_Bot2011_COI	GC16	COI	45	BC05868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC16	CTTTAATTACAGCGTTTTTATTGTTGT	151.27295
BC05869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.835	WGS84	BC05869	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05869_Mgig_Bot2012_COI	GC18	1	BC05869_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05869	Sample	BC05869	targeted	Absent	Sample	Mgig_Bot2012_COI	GC18	COI	45	BC05869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC18	TCTTATTCGTTGGAGACTTTATAACCCT	151.27193
BC05871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83199	WGS84	BC05871	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05871_Aamu_Bax2006_COI	GC21	1	BC05871_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05871	Sample	BC05871	targeted	Absent	Sample	Aamu_Bax2006_COI	GC21	COI	45	BC05871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC21	GCACAACCGGGATCTTTACTTC	151.25933
BC05871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83199	WGS84	BC05871	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05871_Pcan_Bot2011_IGS	GC21	1	BC05871_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05871	Sample	BC05871	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC21	IGS	45	BC05871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC21	CGTAATCCTCAGTACTGGCTA	151.25933
BC05871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83199	WGS84	BC05871	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05871_Sspa_Oph2007_COI	GC21	1	BC05871_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05871	Sample	BC05871	targeted	Absent	Sample	Sspa_Oph2007_COI	GC21	COI	45	BC05871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC21	GAAGGCCTTCCGATGATC	151.25933
BC05871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83199	WGS84	BC05871	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05871_Upin_Bot2011_COI	GC21	1	BC05871_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05871	Sample	BC05871	targeted	Absent	Sample	Upin_Bot2011_COI	GC21	COI	45	BC05871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC21	CTTTAATTACAGCGTTTTTATTGTTGT	151.25933
BC05872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83097	WGS84	BC05872	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05872_Mgig_Bot2012_COI	GC23	1	BC05872_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05872	Sample	BC05872	targeted	Absent	Sample	Mgig_Bot2012_COI	GC23	COI	45	BC05872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC23	TCTTATTCGTTGGAGACTTTATAACCCT	151.2546
BC05873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83485	WGS84	BC05873	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05873_Cmae_Bot2011_COI	GC34	1	BC05873_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05873	Sample	BC05873	targeted	Absent	Sample	Cmae_Bot2011_COI	GC34	COI	45	BC05873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC34	ATGAACAGTCTATCCTCCTTTAG	151.26891
BC05873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.83485	WGS84	BC05873	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05873_Msal_Bot2012_COI	GC34	1	BC05873_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05873	Sample	BC05873	targeted	Absent	Sample	Msal_Bot2012_COI	GC34	COI	45	BC05873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC34	GYTAGTTCCRATGATGTTAGCTG	151.26891
BC05875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79288	WGS84	BC05875	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05875_Aamu_Bax2006_COI	GC71	1	BC05875_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05875	Sample	BC05875	targeted	Absent	Sample	Aamu_Bax2006_COI	GC71	COI	45	BC05875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC71	GCACAACCGGGATCTTTACTTC	151.2117
BC05875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79288	WGS84	BC05875	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05875_Pcan_Bot2011_IGS	GC71	1	BC05875_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05875	Sample	BC05875	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC71	IGS	45	BC05875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC71	CGTAATCCTCAGTACTGGCTA	151.2117
BC05875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79288	WGS84	BC05875	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05875_Sspa_Oph2007_COI	GC71	1	BC05875_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05875	Sample	BC05875	targeted	Absent	Sample	Sspa_Oph2007_COI	GC71	COI	45	BC05875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC71	GAAGGCCTTCCGATGATC	151.2117
BC05875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79288	WGS84	BC05875	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05875_Upin_Bot2011_COI	GC71	1	BC05875_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05875	Sample	BC05875	targeted	Absent	Sample	Upin_Bot2011_COI	GC71	COI	45	BC05875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC71	CTTTAATTACAGCGTTTTTATTGTTGT	151.2117
BC05876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79447	WGS84	BC05876	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05876_Mgig_Bot2012_COI	GC72	1	BC05876_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05876	Sample	BC05876	targeted	Absent	Sample	Mgig_Bot2012_COI	GC72	COI	45	BC05876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC72	TCTTATTCGTTGGAGACTTTATAACCCT	151.21339
BC05877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79074	WGS84	BC05877	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05877_Cmae_Bot2011_COI	GC73	1	BC05877_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05877	Sample	BC05877	targeted	Absent	Sample	Cmae_Bot2011_COI	GC73	COI	45	BC05877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC73	ATGAACAGTCTATCCTCCTTTAG	151.20948
BC05877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.79074	WGS84	BC05877	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05877_Msal_Bot2012_COI	GC73	1	BC05877_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05877	Sample	BC05877	targeted	Absent	Sample	Msal_Bot2012_COI	GC73	COI	45	BC05877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC73	GYTAGTTCCRATGATGTTAGCTG	151.20948
BC05879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81491	WGS84	BC05879	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05879_Aamu_Bax2006_COI	GC26	1	BC05879_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05879	Sample	BC05879	targeted	Absent	Sample	Aamu_Bax2006_COI	GC26	COI	45	BC05879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC26	GCACAACCGGGATCTTTACTTC	151.23674
BC05879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81491	WGS84	BC05879	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05879_Pcan_Bot2011_IGS	GC26	1	BC05879_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05879	Sample	BC05879	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC26	IGS	45	BC05879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC26	CGTAATCCTCAGTACTGGCTA	151.23674
BC05879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81491	WGS84	BC05879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05879_Sspa_Oph2007_COI	GC26	1	BC05879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05879	Sample	BC05879	targeted	Absent	Sample	Sspa_Oph2007_COI	GC26	COI	45	BC05879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC26	GAAGGCCTTCCGATGATC	151.23674
BC05879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81491	WGS84	BC05879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05879_Upin_Bot2011_COI	GC26	1	BC05879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05879	Sample	BC05879	targeted	Absent	Sample	Upin_Bot2011_COI	GC26	COI	45	BC05879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC26	CTTTAATTACAGCGTTTTTATTGTTGT	151.23674
BC05880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81635	WGS84	BC05880	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05880_Cmae_Bot2011_COI	GC27	1	BC05880_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05880	Sample	BC05880	targeted	Absent	Sample	Cmae_Bot2011_COI	GC27	COI	45	BC05880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC27	ATGAACAGTCTATCCTCCTTTAG	151.23642
BC05880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81635	WGS84	BC05880	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05880_Msal_Bot2012_COI	GC27	1	BC05880_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05880	Sample	BC05880	targeted	Absent	Sample	Msal_Bot2012_COI	GC27	COI	45	BC05880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC27	GYTAGTTCCRATGATGTTAGCTG	151.23642
BC05882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82101	WGS84	BC05882	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05882_Aamu_Bax2006_COI	GC31	1	BC05882_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05882	Sample	BC05882	targeted	Absent	Sample	Aamu_Bax2006_COI	GC31	COI	45	BC05882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC31	GCACAACCGGGATCTTTACTTC	151.24208
BC05882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82101	WGS84	BC05882	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05882_Pcan_Bot2011_IGS	GC31	1	BC05882_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05882	Sample	BC05882	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC31	IGS	45	BC05882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC31	CGTAATCCTCAGTACTGGCTA	151.24208
BC05882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82101	WGS84	BC05882	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05882_Sspa_Oph2007_COI	GC31	1	BC05882_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05882	Sample	BC05882	targeted	Absent	Sample	Sspa_Oph2007_COI	GC31	COI	45	BC05882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC31	GAAGGCCTTCCGATGATC	151.24208
BC05882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82101	WGS84	BC05882	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05882_Upin_Bot2011_COI	GC31	1	BC05882_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05882	Sample	BC05882	targeted	Absent	Sample	Upin_Bot2011_COI	GC31	COI	45	BC05882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC31	CTTTAATTACAGCGTTTTTATTGTTGT	151.24208
BC05883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82391	WGS84	BC05883	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05883_Mgig_Bot2012_COI	GC32	1	BC05883_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05883	Sample	BC05883	targeted	Absent	Sample	Mgig_Bot2012_COI	GC32	COI	45	BC05883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC32	TCTTATTCGTTGGAGACTTTATAACCCT	151.24455
BC05884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82267	WGS84	BC05884	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05884_Cmae_Bot2011_COI	GC42	1	BC05884_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05884	Sample	BC05884	targeted	Absent	Sample	Cmae_Bot2011_COI	GC42	COI	45	BC05884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC42	ATGAACAGTCTATCCTCCTTTAG	151.24423
BC05884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82267	WGS84	BC05884	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05884_Msal_Bot2012_COI	GC42	1	BC05884_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05884	Sample	BC05884	targeted	Absent	Sample	Msal_Bot2012_COI	GC42	COI	45	BC05884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC42	GYTAGTTCCRATGATGTTAGCTG	151.24423
BC05886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8224	WGS84	BC05886	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05886_Aamu_Bax2006_COI	GC49	1	BC05886_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05886	Sample	BC05886	targeted	Absent	Sample	Aamu_Bax2006_COI	GC49	COI	45	BC05886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC49	GCACAACCGGGATCTTTACTTC	151.24152
BC05886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8224	WGS84	BC05886	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05886_Pcan_Bot2011_IGS	GC49	1	BC05886_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05886	Sample	BC05886	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC49	IGS	45	BC05886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC49	CGTAATCCTCAGTACTGGCTA	151.24152
BC05886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8224	WGS84	BC05886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05886_Sspa_Oph2007_COI	GC49	1	BC05886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05886	Sample	BC05886	targeted	Absent	Sample	Sspa_Oph2007_COI	GC49	COI	45	BC05886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC49	GAAGGCCTTCCGATGATC	151.24152
BC05886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.8224	WGS84	BC05886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05886_Upin_Bot2011_COI	GC49	1	BC05886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05886	Sample	BC05886	targeted	Absent	Sample	Upin_Bot2011_COI	GC49	COI	45	BC05886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC49	CTTTAATTACAGCGTTTTTATTGTTGT	151.24152
BC05887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82011	WGS84	BC05887	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05887_Mgig_Bot2012_COI	GC51	1	BC05887_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05887	Sample	BC05887	targeted	Absent	Sample	Mgig_Bot2012_COI	GC51	COI	45	BC05887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC51	TCTTATTCGTTGGAGACTTTATAACCCT	151.23989
BC05888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81964	WGS84	BC05888	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05888_Cmae_Bot2011_COI	GC53	1	BC05888_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05888	Sample	BC05888	targeted	Absent	Sample	Cmae_Bot2011_COI	GC53	COI	45	BC05888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC53	ATGAACAGTCTATCCTCCTTTAG	151.23886
BC05888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81964	WGS84	BC05888	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05888_Msal_Bot2012_COI	GC53	1	BC05888_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05888	Sample	BC05888	targeted	Absent	Sample	Msal_Bot2012_COI	GC53	COI	45	BC05888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC53	GYTAGTTCCRATGATGTTAGCTG	151.23886
BC05890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82703	WGS84	BC05890	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05890_Mgig_Bot2012_COI	GC59	1	BC05890_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05890	Sample	BC05890	targeted	Absent	Sample	Mgig_Bot2012_COI	GC59	COI	45	BC05890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC59	TCTTATTCGTTGGAGACTTTATAACCCT	151.24639
BC05891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82553	WGS84	BC05891	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05891_Cmae_Bot2011_COI	GC61	1	BC05891_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05891	Sample	BC05891	targeted	Absent	Sample	Cmae_Bot2011_COI	GC61	COI	45	BC05891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC61	ATGAACAGTCTATCCTCCTTTAG	151.2492
BC05891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.82553	WGS84	BC05891	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05891_Msal_Bot2012_COI	GC61	1	BC05891_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05891	Sample	BC05891	targeted	Absent	Sample	Msal_Bot2012_COI	GC61	COI	45	BC05891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC61	GYTAGTTCCRATGATGTTAGCTG	151.2492
BC05893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81925	WGS84	BC05893	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05893_Aamu_Bax2006_COI	GC63	1	BC05893_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05893	Sample	BC05893	targeted	Absent	Sample	Aamu_Bax2006_COI	GC63	COI	45	BC05893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC63	GCACAACCGGGATCTTTACTTC	151.24293
BC05893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81925	WGS84	BC05893	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05893_Pcan_Bot2011_IGS	GC63	1	BC05893_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05893	Sample	BC05893	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC63	IGS	45	BC05893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC63	CGTAATCCTCAGTACTGGCTA	151.24293
BC05893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81925	WGS84	BC05893	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05893_Sspa_Oph2007_COI	GC63	1	BC05893_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05893	Sample	BC05893	targeted	Absent	Sample	Sspa_Oph2007_COI	GC63	COI	45	BC05893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC63	GAAGGCCTTCCGATGATC	151.24293
BC05893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81925	WGS84	BC05893	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05893_Upin_Bot2011_COI	GC63	1	BC05893_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05893	Sample	BC05893	targeted	Absent	Sample	Upin_Bot2011_COI	GC63	COI	45	BC05893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC63	CTTTAATTACAGCGTTTTTATTGTTGT	151.24293
BC05894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81761	WGS84	BC05894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05894_Mgig_Bot2012_COI	GC64	1	BC05894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05894	Sample	BC05894	targeted	Absent	Sample	Mgig_Bot2012_COI	GC64	COI	45	BC05894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC64	TCTTATTCGTTGGAGACTTTATAACCCT	151.24103
BC05895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81432	WGS84	BC05895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05895_Cmae_Bot2011_COI	GC66	1	BC05895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05895	Sample	BC05895	targeted	Absent	Sample	Cmae_Bot2011_COI	GC66	COI	45	BC05895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC66	ATGAACAGTCTATCCTCCTTTAG	151.23819
BC05895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.81432	WGS84	BC05895	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05895_Msal_Bot2012_COI	GC66	1	BC05895_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05895	Sample	BC05895	targeted	Absent	Sample	Msal_Bot2012_COI	GC66	COI	45	BC05895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC66	GYTAGTTCCRATGATGTTAGCTG	151.23819
BC05897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78028	WGS84	BC05897	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC05897_Aamu_Bax2006_COI	GC77	1	BC05897_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05897	Sample	BC05897	targeted	Absent	Sample	Aamu_Bax2006_COI	GC77	COI	45	BC05897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC77	GCACAACCGGGATCTTTACTTC	151.19927
BC05897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78028	WGS84	BC05897	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC05897_Pcan_Bot2011_IGS	GC77	1	BC05897_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05897	Sample	BC05897	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC77	IGS	45	BC05897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC77	CGTAATCCTCAGTACTGGCTA	151.19927
BC05897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78028	WGS84	BC05897	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC05897_Sspa_Oph2007_COI	GC77	1	BC05897_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05897	Sample	BC05897	targeted	Absent	Sample	Sspa_Oph2007_COI	GC77	COI	45	BC05897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC77	GAAGGCCTTCCGATGATC	151.19927
BC05897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78028	WGS84	BC05897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC05897_Upin_Bot2011_COI	GC77	1	BC05897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05897	Sample	BC05897	targeted	Absent	Sample	Upin_Bot2011_COI	GC77	COI	45	BC05897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC77	CTTTAATTACAGCGTTTTTATTGTTGT	151.19927
BC05898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.78291	WGS84	BC05898	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC05898_Mgig_Bot2012_COI	GC78	1	BC05898_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05898	Sample	BC05898	targeted	Absent	Sample	Mgig_Bot2012_COI	GC78	COI	45	BC05898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC78	TCTTATTCGTTGGAGACTTTATAACCCT	151.19983
BC05899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.77805	WGS84	BC05899	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC05899_Cmae_Bot2011_COI	GC81	1	BC05899_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05899	Sample	BC05899	targeted	Absent	Sample	Cmae_Bot2011_COI	GC81	COI	45	BC05899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC81	ATGAACAGTCTATCCTCCTTTAG	151.19493
BC05899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10457	-23.77805	WGS84	BC05899	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Prowse T, Deveney MR (2019) Validation of molecular surveillance: parallel traditional and molecular surveys for priority marine pests in the ports of Gladstone, Brisbane, Melbourne and Hobart. Report to Department of Agriculture and Water Resources. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000040-1. SARDI Research Report Series No. 1019., Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC05899_Msal_Bot2012_COI	GC81	1	BC05899_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC05899	Sample	BC05899	targeted	Absent	Sample	Msal_Bot2012_COI	GC81	COI	45	BC05899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC81	GYTAGTTCCRATGATGTTAGCTG	151.19493
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.72	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06766_Aamu_Bax2006_COI	T5-02	1	BC06766_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Present	Sample	Aamu_Bax2006_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	GCACAACCGGGATCTTTACTTC	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06766_Pcan_Bot2011_IGS	T5-02	1	BC06766_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-02	IGS	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	CGTAATCCTCAGTACTGGCTA	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06766_Sspa_Oph2007_COI	T5-02	1	BC06766_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	GAAGGCCTTCCGATGATC	147.81438
BC06766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8894	WGS84	BC06766	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.999	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06766_Upin_Bot2011_COI	T5-02	1	BC06766_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06766	Sample	BC06766	targeted	Present	Sample	Upin_Bot2011_COI	T5-02	COI	45	BC06766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-02	CTTTAATTACAGCGTTTTTATTGTTGT	147.81438
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06767_Cjap_Sim2018_COI	T5-03	1	BC06767_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87443
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	38.58	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	17/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06767_Mgig_Bot2012_COI	T5-03	1	BC06767_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Present	Sample	Mgig_Bot2012_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.87443
BC06767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84861	WGS84	BC06767	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06767_Mjap_Gib2022_COI	T5-03	1	BC06767_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06767	Sample	BC06767	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-03	COI	45	BC06767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-03	AATTTAATTGTGACTAGGCATGGTCTG	147.87443
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06768_Cmae_Bot2011_COI	T5-04	1	BC06768_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	ATGAACAGTCTATCCTCCTTTAG	147.87403
BC06768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85093	WGS84	BC06768	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.465	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06768_Mros_Gib2022_COI	T5-04	1	BC06768_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06768	Sample	BC06768	targeted	Present	Sample	Mros_Gib2022_COI	T5-04	COI	45	BC06768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87403
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06770_Cjap_Sim2018_COI	T5-06	1	BC06770_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87262
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	17/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06770_Mgig_Bot2012_COI	T5-06	1	BC06770_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.87262
BC06770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85664	WGS84	BC06770	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	17/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06770_Mjap_Gib2022_COI	T5-06	1	BC06770_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06770	Sample	BC06770	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-06	COI	45	BC06770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-06	AATTTAATTGTGACTAGGCATGGTCTG	147.87262
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06771_Cmae_Bot2011_COI	T5-07	1	BC06771_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	ATGAACAGTCTATCCTCCTTTAG	147.30203
BC06771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06771	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06771_Mros_Gib2022_COI	T5-07	1	BC06771_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06771	Sample	BC06771	targeted	Absent	Sample	Mros_Gib2022_COI	T5-07	COI	45	BC06771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30203
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.581	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06773_Aamu_Bax2006_COI	T5-09	1	BC06773_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Present	Sample	Aamu_Bax2006_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	GCACAACCGGGATCTTTACTTC	147.30444
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06773_Pcan_Bot2011_IGS	T5-09	1	BC06773_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-09	IGS	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	CGTAATCCTCAGTACTGGCTA	147.30444
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06773_Sspa_Oph2007_COI	T5-09	1	BC06773_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	GAAGGCCTTCCGATGATC	147.30444
BC06773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82558	WGS84	BC06773	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06773_Upin_Bot2011_COI	T5-09	1	BC06773_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06773	Sample	BC06773	targeted	Absent	Sample	Upin_Bot2011_COI	T5-09	COI	45	BC06773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-09	CTTTAATTACAGCGTTTTTATTGTTGT	147.30444
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06774_Cjap_Sim2018_COI	T5-10	1	BC06774_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33697
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06774_Mgig_Bot2012_COI	T5-10	1	BC06774_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	TCTTATTCGTTGGAGACTTTATAACCCT	147.33697
BC06774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06774	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06774_Mjap_Gib2022_COI	T5-10	1	BC06774_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06774	Sample	BC06774	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-10	COI	45	BC06774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-10	AATTTAATTGTGACTAGGCATGGTCTG	147.33697
BC06775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87812	WGS84	BC06775	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.224	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06775_Cmae_Bot2011_COI	T5-11	1	BC06775_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06775	Sample	BC06775	targeted	Present	Sample	Cmae_Bot2011_COI	T5-11	COI	45	BC06775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-11	ATGAACAGTCTATCCTCCTTTAG	147.34163
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.145	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06777_Aamu_Bax2006_COI	T5-13	1	BC06777_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Present	Sample	Aamu_Bax2006_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	GCACAACCGGGATCTTTACTTC	147.33945
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06777_Pcan_Bot2011_IGS	T5-13	1	BC06777_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-13	IGS	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	CGTAATCCTCAGTACTGGCTA	147.33945
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06777_Sspa_Oph2007_COI	T5-13	1	BC06777_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	GAAGGCCTTCCGATGATC	147.33945
BC06777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.8833	WGS84	BC06777	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06777_Upin_Bot2011_COI	T5-13	1	BC06777_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06777	Sample	BC06777	targeted	Absent	Sample	Upin_Bot2011_COI	T5-13	COI	45	BC06777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-13	CTTTAATTACAGCGTTTTTATTGTTGT	147.33945
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06778_Cjap_Sim2018_COI	T5-14	1	BC06778_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33702
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.189	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06778_Mgig_Bot2012_COI	T5-14	1	BC06778_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Present	Sample	Mgig_Bot2012_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.33702
BC06778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88555	WGS84	BC06778	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06778_Mjap_Gib2022_COI	T5-14	1	BC06778_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06778	Sample	BC06778	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-14	COI	45	BC06778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-14	AATTTAATTGTGACTAGGCATGGTCTG	147.33702
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06779_Cmae_Bot2011_COI	T5-15	1	BC06779_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Absent	Sample	Cmae_Bot2011_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	ATGAACAGTCTATCCTCCTTTAG	147.53158
BC06779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96047	WGS84	BC06779	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	21/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06779_Mros_Gib2022_COI	T5-15	1	BC06779_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06779	Sample	BC06779	targeted	Absent	Sample	Mros_Gib2022_COI	T5-15	COI	45	BC06779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-15	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53158
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.095	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06780_Aamu_Bax2006_COI	T5-16	1	BC06780_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Present	Sample	Aamu_Bax2006_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	GCACAACCGGGATCTTTACTTC	147.53983
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06780_Pcan_Bot2011_IGS	T5-16	1	BC06780_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Absent	Sample	Pcan_Bot2011_IGS	T5-16	IGS	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	CGTAATCCTCAGTACTGGCTA	147.53983
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06780_Sspa_Oph2007_COI	T5-16	1	BC06780_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Absent	Sample	Sspa_Oph2007_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	GAAGGCCTTCCGATGATC	147.53983
BC06780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9618	WGS84	BC06780	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06780_Upin_Bot2011_COI	T5-16	1	BC06780_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06780	Sample	BC06780	targeted	Absent	Sample	Upin_Bot2011_COI	T5-16	COI	45	BC06780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.53983
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06781_Cjap_Sim2018_COI	T5-17	1	BC06781_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Absent	Sample	Cjap_Sim2018_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33607
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T05	19/10/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06781_Mgig_Bot2012_COI	T5-17	1	BC06781_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Absent	Sample	Mgig_Bot2012_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	TCTTATTCGTTGGAGACTTTATAACCCT	147.33607
BC06781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88462	WGS84	BC06781	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	19/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06781_Mjap_Gib2022_COI	T5-17	1	BC06781_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06781	Sample	BC06781	targeted	Absent	Sample	Mjap_Gib2022_COI	T5-17	COI	45	BC06781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-17	AATTTAATTGTGACTAGGCATGGTCTG	147.33607
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.62	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06782_Cmae_Bot2011_COI	T5-18	1	BC06782_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Present	Sample	Cmae_Bot2011_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	ATGAACAGTCTATCCTCCTTTAG	147.53312
BC06782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96113	WGS84	BC06782	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.572	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T05	26/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06782_Mros_Gib2022_COI	T5-18	1	BC06782_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06782	Sample	BC06782	targeted	Present	Sample	Mros_Gib2022_COI	T5-18	COI	45	BC06782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T5-18	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53312
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.223	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06784_Aamu_Bax2006_COI	T6-01	1	BC06784_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Present	Sample	Aamu_Bax2006_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	GCACAACCGGGATCTTTACTTC	147.30679
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06784_Pcan_Bot2011_IGS	T6-01	1	BC06784_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-01	IGS	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	CGTAATCCTCAGTACTGGCTA	147.30679
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06784_Sspa_Oph2007_COI	T6-01	1	BC06784_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	GAAGGCCTTCCGATGATC	147.30679
BC06784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82492	WGS84	BC06784	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06784_Upin_Bot2011_COI	T6-01	1	BC06784_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06784	Sample	BC06784	targeted	Absent	Sample	Upin_Bot2011_COI	T6-01	COI	45	BC06784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.30679
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06785_Cjap_Sim2018_COI	T6-02	1	BC06785_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.303
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.531	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06785_Mgig_Bot2012_COI	T6-02	1	BC06785_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Present	Sample	Mgig_Bot2012_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.303
BC06785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82589	WGS84	BC06785	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06785_Mjap_Gib2022_COI	T6-02	1	BC06785_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06785	Sample	BC06785	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-02	COI	45	BC06785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-02	AATTTAATTGTGACTAGGCATGGTCTG	147.303
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06786_Cmae_Bot2011_COI	T6-03	1	BC06786_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Absent	Sample	Cmae_Bot2011_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	ATGAACAGTCTATCCTCCTTTAG	147.30193
BC06786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82827	WGS84	BC06786	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06786_Mros_Gib2022_COI	T6-03	1	BC06786_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06786	Sample	BC06786	targeted	Absent	Sample	Mros_Gib2022_COI	T6-03	COI	45	BC06786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-03	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30193
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.316	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06788_Aamu_Bax2006_COI	T6-05	1	BC06788_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Present	Sample	Aamu_Bax2006_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	GCACAACCGGGATCTTTACTTC	147.34157
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06788_Pcan_Bot2011_IGS	T6-05	1	BC06788_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-05	IGS	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	CGTAATCCTCAGTACTGGCTA	147.34157
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06788_Sspa_Oph2007_COI	T6-05	1	BC06788_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	GAAGGCCTTCCGATGATC	147.34157
BC06788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88036	WGS84	BC06788	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06788_Upin_Bot2011_COI	T6-05	1	BC06788_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06788	Sample	BC06788	targeted	Absent	Sample	Upin_Bot2011_COI	T6-05	COI	45	BC06788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.34157
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06789_Cjap_Sim2018_COI	T6-06	1	BC06789_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34153
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.591	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06789_Mgig_Bot2012_COI	T6-06	1	BC06789_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Present	Sample	Mgig_Bot2012_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.34153
BC06789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87789	WGS84	BC06789	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06789_Mjap_Gib2022_COI	T6-06	1	BC06789_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06789	Sample	BC06789	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-06	COI	45	BC06789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-06	AATTTAATTGTGACTAGGCATGGTCTG	147.34153
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.37	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06791_Aamu_Bax2006_COI	T6-08	1	BC06791_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Present	Sample	Aamu_Bax2006_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	GCACAACCGGGATCTTTACTTC	147.33618
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06791_Pcan_Bot2011_IGS	T6-08	1	BC06791_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-08	IGS	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	CGTAATCCTCAGTACTGGCTA	147.33618
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06791_Sspa_Oph2007_COI	T6-08	1	BC06791_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	GAAGGCCTTCCGATGATC	147.33618
BC06791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88415	WGS84	BC06791	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06791_Upin_Bot2011_COI	T6-08	1	BC06791_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06791	Sample	BC06791	targeted	Absent	Sample	Upin_Bot2011_COI	T6-08	COI	45	BC06791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06792_Cjap_Sim2018_COI	T6-09	1	BC06792_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.899	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	22/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06792_Mgig_Bot2012_COI	T6-09	1	BC06792_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Present	Sample	Mgig_Bot2012_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.33618
BC06792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88268	WGS84	BC06792	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	22/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06792_Mjap_Gib2022_COI	T6-09	1	BC06792_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06792	Sample	BC06792	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-09	COI	45	BC06792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-09	AATTTAATTGTGACTAGGCATGGTCTG	147.33618
BC06793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.9627	WGS84	BC06793	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.934	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06793_Cmae_Bot2011_COI	T6-10	1	BC06793_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06793	Sample	BC06793	targeted	Present	Sample	Cmae_Bot2011_COI	T6-10	COI	45	BC06793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-10	ATGAACAGTCTATCCTCCTTTAG	147.53935
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06795_Aamu_Bax2006_COI	T6-12	1	BC06795_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Absent	Sample	Aamu_Bax2006_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	GCACAACCGGGATCTTTACTTC	147.51949
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06795_Pcan_Bot2011_IGS	T6-12	1	BC06795_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-12	IGS	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	CGTAATCCTCAGTACTGGCTA	147.51949
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06795_Sspa_Oph2007_COI	T6-12	1	BC06795_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	GAAGGCCTTCCGATGATC	147.51949
BC06795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96263	WGS84	BC06795	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	26/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06795_Upin_Bot2011_COI	T6-12	1	BC06795_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06795	Sample	BC06795	targeted	Absent	Sample	Upin_Bot2011_COI	T6-12	COI	45	BC06795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.51949
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06796_Cjap_Sim2018_COI	T6-13	1	BC06796_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Absent	Sample	Cjap_Sim2018_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81327
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.154	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T06	28/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06796_Mgig_Bot2012_COI	T6-13	1	BC06796_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Present	Sample	Mgig_Bot2012_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.81327
BC06796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88961	WGS84	BC06796	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06796_Mjap_Gib2022_COI	T6-13	1	BC06796_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06796	Sample	BC06796	targeted	Absent	Sample	Mjap_Gib2022_COI	T6-13	COI	45	BC06796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-13	AATTTAATTGTGACTAGGCATGGTCTG	147.81327
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.623	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06797_Cmae_Bot2011_COI	T6-14	1	BC06797_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Present	Sample	Cmae_Bot2011_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	ATGAACAGTCTATCCTCCTTTAG	147.8762
BC06797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84703	WGS84	BC06797	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.132	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06797_Mros_Gib2022_COI	T6-14	1	BC06797_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06797	Sample	BC06797	targeted	Present	Sample	Mros_Gib2022_COI	T6-14	COI	45	BC06797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-14	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.8762
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06799_Aamu_Bax2006_COI	T6-16	1	BC06799_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Absent	Sample	Aamu_Bax2006_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	GCACAACCGGGATCTTTACTTC	147.87236
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06799_Pcan_Bot2011_IGS	T6-16	1	BC06799_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Absent	Sample	Pcan_Bot2011_IGS	T6-16	IGS	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	CGTAATCCTCAGTACTGGCTA	147.87236
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06799_Sspa_Oph2007_COI	T6-16	1	BC06799_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Absent	Sample	Sspa_Oph2007_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	GAAGGCCTTCCGATGATC	147.87236
BC06799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85578	WGS84	BC06799	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.232	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T06	28/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06799_Upin_Bot2011_COI	T6-16	1	BC06799_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06799	Sample	BC06799	targeted	Present	Sample	Upin_Bot2011_COI	T6-16	COI	45	BC06799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T6-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.87236
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06800_Cjap_Sim2018_COI	T7-01	1	BC06800_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.81365
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06800_Mgig_Bot2012_COI	T7-01	1	BC06800_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Absent	Sample	Mgig_Bot2012_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.81365
BC06800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88943	WGS84	BC06800	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06800_Mjap_Gib2022_COI	T7-01	1	BC06800_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06800	Sample	BC06800	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-01	COI	45	BC06800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-01	AATTTAATTGTGACTAGGCATGGTCTG	147.81365
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06801_Cmae_Bot2011_COI	T7-02	1	BC06801_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Absent	Sample	Cmae_Bot2011_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	ATGAACAGTCTATCCTCCTTTAG	147.87573
BC06801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.84744	WGS84	BC06801	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.547	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06801_Mros_Gib2022_COI	T7-02	1	BC06801_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06801	Sample	BC06801	targeted	Present	Sample	Mros_Gib2022_COI	T7-02	COI	45	BC06801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87573
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.696	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06803_Aamu_Bax2006_COI	T7-04	1	BC06803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Present	Sample	Aamu_Bax2006_COI	T7-04	COI	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	GCACAACCGGGATCTTTACTTC	147.87248
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06803_Pcan_Bot2011_IGS	T7-04	1	BC06803_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-04	IGS	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	CGTAATCCTCAGTACTGGCTA	147.87248
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06803_Sspa_Oph2007_COI	T7-04	1	BC06803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-04	COI	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	GAAGGCCTTCCGATGATC	147.87248
BC06803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85595	WGS84	BC06803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.113	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06803_Upin_Bot2011_COI	T7-04	1	BC06803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06803	Sample	BC06803	targeted	Present	Sample	Upin_Bot2011_COI	T7-04	COI	45	BC06803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.87248
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06804_Cjap_Sim2018_COI	T7-05	1	BC06804_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30842
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.342	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06804_Mgig_Bot2012_COI	T7-05	1	BC06804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Present	Sample	Mgig_Bot2012_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.30842
BC06804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82522	WGS84	BC06804	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06804_Mjap_Gib2022_COI	T7-05	1	BC06804_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06804	Sample	BC06804	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-05	COI	45	BC06804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-05	AATTTAATTGTGACTAGGCATGGTCTG	147.30842
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.491	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06805_Cmae_Bot2011_COI	T7-06	1	BC06805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Present	Sample	Cmae_Bot2011_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	ATGAACAGTCTATCCTCCTTTAG	147.30286
BC06805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82654	WGS84	BC06805	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06805_Mros_Gib2022_COI	T7-06	1	BC06805_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06805	Sample	BC06805	targeted	Absent	Sample	Mros_Gib2022_COI	T7-06	COI	45	BC06805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30286
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.875	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06807_Aamu_Bax2006_COI	T7-08	1	BC06807_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Present	Sample	Aamu_Bax2006_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	GCACAACCGGGATCTTTACTTC	147.33611
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06807_Pcan_Bot2011_IGS	T7-08	1	BC06807_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-08	IGS	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	CGTAATCCTCAGTACTGGCTA	147.33611
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06807_Sspa_Oph2007_COI	T7-08	1	BC06807_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	GAAGGCCTTCCGATGATC	147.33611
BC06807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87318	WGS84	BC06807	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06807_Upin_Bot2011_COI	T7-08	1	BC06807_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06807	Sample	BC06807	targeted	Absent	Sample	Upin_Bot2011_COI	T7-08	COI	45	BC06807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.33611
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06808_Cjap_Sim2018_COI	T7-09	1	BC06808_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34178
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06808_Mgig_Bot2012_COI	T7-09	1	BC06808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Absent	Sample	Mgig_Bot2012_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.34178
BC06808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88012	WGS84	BC06808	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06808_Mjap_Gib2022_COI	T7-09	1	BC06808_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06808	Sample	BC06808	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-09	COI	45	BC06808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-09	AATTTAATTGTGACTAGGCATGGTCTG	147.34178
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06809_Cmae_Bot2011_COI	T7-10	1	BC06809_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Absent	Sample	Cmae_Bot2011_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	ATGAACAGTCTATCCTCCTTTAG	147.34175
BC06809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.87932	WGS84	BC06809	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	36.082	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06809_Mros_Gib2022_COI	T7-10	1	BC06809_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06809	Sample	BC06809	targeted	Present	Sample	Mros_Gib2022_COI	T7-10	COI	45	BC06809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-10	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.34175
BC06810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88559	WGS84	BC06810	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.393	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06810_Aamu_Bax2006_COI	T7-11	1	BC06810_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06810	Sample	BC06810	targeted	Present	Sample	Aamu_Bax2006_COI	T7-11	COI	45	BC06810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-11	GCACAACCGGGATCTTTACTTC	147.33519
BC06810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88559	WGS84	BC06810	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06810_Pcan_Bot2011_IGS	T7-11	1	BC06810_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06810	Sample	BC06810	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-11	IGS	45	BC06810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-11	CGTAATCCTCAGTACTGGCTA	147.33519
BC06810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88559	WGS84	BC06810	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06810_Sspa_Oph2007_COI	T7-11	1	BC06810_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06810	Sample	BC06810	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-11	COI	45	BC06810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-11	GAAGGCCTTCCGATGATC	147.33519
BC06810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88559	WGS84	BC06810	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06810_Upin_Bot2011_COI	T7-11	1	BC06810_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06810	Sample	BC06810	targeted	Absent	Sample	Upin_Bot2011_COI	T7-11	COI	45	BC06810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33519
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06811_Cjap_Sim2018_COI	T7-12	1	BC06811_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.33607
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.965	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	12/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06811_Mgig_Bot2012_COI	T7-12	1	BC06811_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Present	Sample	Mgig_Bot2012_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	TCTTATTCGTTGGAGACTTTATAACCCT	147.33607
BC06811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88421	WGS84	BC06811	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06811_Mjap_Gib2022_COI	T7-12	1	BC06811_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06811	Sample	BC06811	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-12	COI	45	BC06811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-12	AATTTAATTGTGACTAGGCATGGTCTG	147.33607
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.067	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06812_Cmae_Bot2011_COI	T7-13	1	BC06812_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Present	Sample	Cmae_Bot2011_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	ATGAACAGTCTATCCTCCTTTAG	147.33619
BC06812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88258	WGS84	BC06812	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.123	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	12/12/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06812_Mros_Gib2022_COI	T7-13	1	BC06812_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06812	Sample	BC06812	targeted	Present	Sample	Mros_Gib2022_COI	T7-13	COI	45	BC06812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-13	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33619
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.236	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06814_Aamu_Bax2006_COI	T7-15	1	BC06814_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Present	Sample	Aamu_Bax2006_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	GCACAACCGGGATCTTTACTTC	147.53178
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06814_Pcan_Bot2011_IGS	T7-15	1	BC06814_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Absent	Sample	Pcan_Bot2011_IGS	T7-15	IGS	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	CGTAATCCTCAGTACTGGCTA	147.53178
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06814_Sspa_Oph2007_COI	T7-15	1	BC06814_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Absent	Sample	Sspa_Oph2007_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	GAAGGCCTTCCGATGATC	147.53178
BC06814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96027	WGS84	BC06814	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06814_Upin_Bot2011_COI	T7-15	1	BC06814_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06814	Sample	BC06814	targeted	Absent	Sample	Upin_Bot2011_COI	T7-15	COI	45	BC06814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.53178
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06815_Cjap_Sim2018_COI	T7-16	1	BC06815_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Absent	Sample	Cjap_Sim2018_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51962
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.151	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T07	28/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06815_Mgig_Bot2012_COI	T7-16	1	BC06815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Present	Sample	Mgig_Bot2012_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.51962
BC06815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96388	WGS84	BC06815	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T07	28/12/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06815_Mjap_Gib2022_COI	T7-16	1	BC06815_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06815	Sample	BC06815	targeted	Absent	Sample	Mjap_Gib2022_COI	T7-16	COI	45	BC06815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T7-16	AATTTAATTGTGACTAGGCATGGTCTG	147.51962
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.742	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06816_Cmae_Bot2011_COI	T8-01	1	BC06816_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Present	Sample	Cmae_Bot2011_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	ATGAACAGTCTATCCTCCTTTAG	147.81263
BC06816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88962	WGS84	BC06816	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.384	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06816_Mros_Gib2022_COI	T8-01	1	BC06816_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06816	Sample	BC06816	targeted	Present	Sample	Mros_Gib2022_COI	T8-01	COI	45	BC06816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.81263
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06818_Aamu_Bax2006_COI	T8-03	1	BC06818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Absent	Sample	Aamu_Bax2006_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	GCACAACCGGGATCTTTACTTC	147.87373
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06818_Pcan_Bot2011_IGS	T8-03	1	BC06818_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-03	IGS	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	CGTAATCCTCAGTACTGGCTA	147.87373
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06818_Sspa_Oph2007_COI	T8-03	1	BC06818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	GAAGGCCTTCCGATGATC	147.87373
BC06818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85067	WGS84	BC06818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06818_Upin_Bot2011_COI	T8-03	1	BC06818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06818	Sample	BC06818	targeted	Absent	Sample	Upin_Bot2011_COI	T8-03	COI	45	BC06818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.87373
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	17/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06819_Cjap_Sim2018_COI	T8-04	1	BC06819_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-04	COI	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.85574
BC06819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.85592	WGS84	BC06819	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.308	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	17/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06819_Mgig_Bot2012_COI	T8-04	1	BC06819_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06819	Sample	BC06819	targeted	Present	Sample	Mgig_Bot2012_COI	T8-04	COI	45	BC06819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.85574
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.679	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06821_Aamu_Bax2006_COI	T8-06	1	BC06821_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Present	Sample	Aamu_Bax2006_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	GCACAACCGGGATCTTTACTTC	147.3021
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06821_Pcan_Bot2011_IGS	T8-06	1	BC06821_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-06	IGS	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	CGTAATCCTCAGTACTGGCTA	147.3021
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06821_Sspa_Oph2007_COI	T8-06	1	BC06821_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	GAAGGCCTTCCGATGATC	147.3021
BC06821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82878	WGS84	BC06821	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06821_Upin_Bot2011_COI	T8-06	1	BC06821_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06821	Sample	BC06821	targeted	Absent	Sample	Upin_Bot2011_COI	T8-06	COI	45	BC06821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.3021
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06822_Cjap_Sim2018_COI	T8-07	1	BC06822_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30214
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.171	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06822_Mgig_Bot2012_COI	T8-07	1	BC06822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Present	Sample	Mgig_Bot2012_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.30214
BC06822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82752	WGS84	BC06822	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06822_Mjap_Gib2022_COI	T8-07	1	BC06822_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06822	Sample	BC06822	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-07	COI	45	BC06822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-07	AATTTAATTGTGACTAGGCATGGTCTG	147.30214
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.747	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06823_Cmae_Bot2011_COI	T8-08	1	BC06823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Present	Sample	Cmae_Bot2011_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	ATGAACAGTCTATCCTCCTTTAG	147.30793
BC06823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.82546	WGS84	BC06823	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06823_Mros_Gib2022_COI	T8-08	1	BC06823_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06823	Sample	BC06823	targeted	Absent	Sample	Mros_Gib2022_COI	T8-08	COI	45	BC06823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30793
BC06825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88173	WGS84	BC06825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.009	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06825_Aamu_Bax2006_COI	T8-10	1	BC06825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06825	Sample	BC06825	targeted	Present	Sample	Aamu_Bax2006_COI	T8-10	COI	45	BC06825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-10	GCACAACCGGGATCTTTACTTC	147.34203
BC06825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88173	WGS84	BC06825	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06825_Pcan_Bot2011_IGS	T8-10	1	BC06825_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06825	Sample	BC06825	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-10	IGS	45	BC06825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-10	CGTAATCCTCAGTACTGGCTA	147.34203
BC06825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88173	WGS84	BC06825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06825_Sspa_Oph2007_COI	T8-10	1	BC06825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06825	Sample	BC06825	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-10	COI	45	BC06825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-10	GAAGGCCTTCCGATGATC	147.34203
BC06825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88173	WGS84	BC06825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06825_Upin_Bot2011_COI	T8-10	1	BC06825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06825	Sample	BC06825	targeted	Absent	Sample	Upin_Bot2011_COI	T8-10	COI	45	BC06825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.34203
BC06826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88087	WGS84	BC06826	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06826_Cjap_Sim2018_COI	T8-11	1	BC06826_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06826	Sample	BC06826	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-11	COI	45	BC06826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34167
BC06826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88087	WGS84	BC06826	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	23.782	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	18/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06826_Mgig_Bot2012_COI	T8-11	1	BC06826_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06826	Sample	BC06826	targeted	Present	Sample	Mgig_Bot2012_COI	T8-11	COI	45	BC06826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-11	TCTTATTCGTTGGAGACTTTATAACCCT	147.34167
BC06827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88574	WGS84	BC06827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06827_Cmae_Bot2011_COI	T8-12	1	BC06827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06827	Sample	BC06827	targeted	Absent	Sample	Cmae_Bot2011_COI	T8-12	COI	45	BC06827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-12	ATGAACAGTCTATCCTCCTTTAG	147.3353
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	38.211	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06829_Aamu_Bax2006_COI	T8-14	1	BC06829_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Present	Sample	Aamu_Bax2006_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	GCACAACCGGGATCTTTACTTC	147.33624
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06829_Pcan_Bot2011_IGS	T8-14	1	BC06829_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-14	IGS	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	CGTAATCCTCAGTACTGGCTA	147.33624
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06829_Sspa_Oph2007_COI	T8-14	1	BC06829_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	GAAGGCCTTCCGATGATC	147.33624
BC06829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.88265	WGS84	BC06829	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	18/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06829_Upin_Bot2011_COI	T8-14	1	BC06829_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06829	Sample	BC06829	targeted	Absent	Sample	Upin_Bot2011_COI	T8-14	COI	45	BC06829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.33624
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.078	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC06830_Cmae_Bot2011_COI	T8-15	1	BC06830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Present	Sample	Cmae_Bot2011_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	ATGAACAGTCTATCCTCCTTTAG	147.53978
BC06830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96233	WGS84	BC06830	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	25.423	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC06830_Mros_Gib2022_COI	T8-15	1	BC06830_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06830	Sample	BC06830	targeted	Present	Sample	Mros_Gib2022_COI	T8-15	COI	45	BC06830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-15	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.53978
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC06832_Aamu_Bax2006_COI	T8-17	1	BC06832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Absent	Sample	Aamu_Bax2006_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	GCACAACCGGGATCTTTACTTC	147.53022
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC06832_Pcan_Bot2011_IGS	T8-17	1	BC06832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Absent	Sample	Pcan_Bot2011_IGS	T8-17	IGS	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	CGTAATCCTCAGTACTGGCTA	147.53022
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC06832_Sspa_Oph2007_COI	T8-17	1	BC06832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Absent	Sample	Sspa_Oph2007_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	GAAGGCCTTCCGATGATC	147.53022
BC06832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96057	WGS84	BC06832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC06832_Upin_Bot2011_COI	T8-17	1	BC06832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06832	Sample	BC06832	targeted	Absent	Sample	Upin_Bot2011_COI	T8-17	COI	45	BC06832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-17	CTTTAATTACAGCGTTTTTATTGTTGT	147.53022
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC06833_Cjap_Sim2018_COI	T8-18	1	BC06833_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Absent	Sample	Cjap_Sim2018_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53883
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	25.123	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T08	28/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC06833_Mgig_Bot2012_COI	T8-18	1	BC06833_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Present	Sample	Mgig_Bot2012_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	TCTTATTCGTTGGAGACTTTATAACCCT	147.53883
BC06833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_10480	-42.96296	WGS84	BC06833	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T08	28/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC06833_Mjap_Gib2022_COI	T8-18	1	BC06833_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC06833	Sample	BC06833	targeted	Absent	Sample	Mjap_Gib2022_COI	T8-18	COI	45	BC06833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T8-18	AATTTAATTGTGACTAGGCATGGTCTG	147.53883
BC11604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.8368	WGS84	BC11604	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.028	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11604_Mgig_Bot2012_COI	S10-03	1	BC11604_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11604	Sample	BC11604	targeted	Present	Sample	Mgig_Bot2012_COI	S10-03	COI	45	BC11604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.50892
BC11608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78682	WGS84	BC11608	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.37	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11608_Mgig_Bot2012_COI	S10-11	1	BC11608_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11608	Sample	BC11608	targeted	Present	Sample	Mgig_Bot2012_COI	S10-11	COI	45	BC11608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51472
BC11611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84708	WGS84	BC11611	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.576	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S10	23/10/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11611_Mgig_Bot2012_COI	S10-06	1	BC11611_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11611	Sample	BC11611	targeted	Present	Sample	Mgig_Bot2012_COI	S10-06	COI	45	BC11611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S10-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.49757
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.751	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11614_Aamu_Bax2006_COI	T15-16	1	BC11614_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Present	Sample	Aamu_Bax2006_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	GCACAACCGGGATCTTTACTTC	147.53792
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11614_Pcan_Bot2011_IGS	T15-16	1	BC11614_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-16	IGS	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	CGTAATCCTCAGTACTGGCTA	147.53792
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11614_Sspa_Oph2007_COI	T15-16	1	BC11614_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	GAAGGCCTTCCGATGATC	147.53792
BC11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96348	WGS84	BC11614	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.325	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11614_Upin_Bot2011_COI	T15-16	1	BC11614_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11614	Sample	BC11614	targeted	Present	Sample	Upin_Bot2011_COI	T15-16	COI	45	BC11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-16	CTTTAATTACAGCGTTTTTATTGTTGT	147.53792
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11615_Cjap_Sim2018_COI	T16-01	1	BC11615_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53922
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11615_Mgig_Bot2012_COI	T16-01	1	BC11615_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Absent	Sample	Mgig_Bot2012_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.53922
BC11615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96295	WGS84	BC11615	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11615_Mjap_Gib2022_COI	T16-01	1	BC11615_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11615	Sample	BC11615	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-01	COI	45	BC11615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-01	AATTTAATTGTGACTAGGCATGGTCTG	147.53922
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.245	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11616_Cmae_Bot2011_COI	T16-02	1	BC11616_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Present	Sample	Cmae_Bot2011_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	ATGAACAGTCTATCCTCCTTTAG	147.52991
BC11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96002	WGS84	BC11616	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.038	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11616_Mros_Gib2022_COI	T16-02	1	BC11616_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11616	Sample	BC11616	targeted	Present	Sample	Mros_Gib2022_COI	T16-02	COI	45	BC11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.52991
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.607	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11618_Aamu_Bax2006_COI	T16-04	1	BC11618_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Present	Sample	Aamu_Bax2006_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	GCACAACCGGGATCTTTACTTC	147.51987
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11618_Pcan_Bot2011_IGS	T16-04	1	BC11618_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-04	IGS	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	CGTAATCCTCAGTACTGGCTA	147.51987
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11618_Sspa_Oph2007_COI	T16-04	1	BC11618_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	GAAGGCCTTCCGATGATC	147.51987
BC11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96499	WGS84	BC11618	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11618_Upin_Bot2011_COI	T16-04	1	BC11618_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11618	Sample	BC11618	targeted	Absent	Sample	Upin_Bot2011_COI	T16-04	COI	45	BC11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.51987
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11619_Cjap_Sim2018_COI	T16-05	1	BC11619_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.46533
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11619_Mgig_Bot2012_COI	T16-05	1	BC11619_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Mgig_Bot2012_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	TCTTATTCGTTGGAGACTTTATAACCCT	147.46533
BC11619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79953	WGS84	BC11619	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11619_Mjap_Gib2022_COI	T16-05	1	BC11619_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11619	Sample	BC11619	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-05	COI	45	BC11619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-05	AATTTAATTGTGACTAGGCATGGTCTG	147.46533
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.808	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11621_Aamu_Bax2006_COI	T16-07	1	BC11621_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Present	Sample	Aamu_Bax2006_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	GCACAACCGGGATCTTTACTTC	147.50225
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11621_Pcan_Bot2011_IGS	T16-07	1	BC11621_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Absent	Sample	Pcan_Bot2011_IGS	T16-07	IGS	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	CGTAATCCTCAGTACTGGCTA	147.50225
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11621_Sspa_Oph2007_COI	T16-07	1	BC11621_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Absent	Sample	Sspa_Oph2007_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	GAAGGCCTTCCGATGATC	147.50225
BC11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81014	WGS84	BC11621	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11621_Upin_Bot2011_COI	T16-07	1	BC11621_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11621	Sample	BC11621	targeted	Absent	Sample	Upin_Bot2011_COI	T16-07	COI	45	BC11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.50225
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11622_Cjap_Sim2018_COI	T16-08	1	BC11622_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Absent	Sample	Cjap_Sim2018_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52327
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T16	10/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11622_Mgig_Bot2012_COI	T16-08	1	BC11622_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Absent	Sample	Mgig_Bot2012_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.52327
BC11622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.80343	WGS84	BC11622	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T16	10/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11622_Mjap_Gib2022_COI	T16-08	1	BC11622_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11622	Sample	BC11622	targeted	Absent	Sample	Mjap_Gib2022_COI	T16-08	COI	45	BC11622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T16-08	AATTTAATTGTGACTAGGCATGGTCTG	147.52327
BC11626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.84556	WGS84	BC11626	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.135	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11626_Mgig_Bot2012_COI	S11-04	1	BC11626_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11626	Sample	BC11626	targeted	Present	Sample	Mgig_Bot2012_COI	S11-04	COI	45	BC11626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49677
BC11633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80952	WGS84	BC11633	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.145	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S11	26/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11633_Mgig_Bot2012_COI	S11-11	1	BC11633_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11633	Sample	BC11633	targeted	Present	Sample	Mgig_Bot2012_COI	S11-11	COI	45	BC11633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S11-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51928
BC11637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.8434	WGS84	BC11637	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.527	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11637_Mgig_Bot2012_COI	S12-03	1	BC11637_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11637	Sample	BC11637	targeted	Present	Sample	Mgig_Bot2012_COI	S12-03	COI	45	BC11637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.49705
BC11640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.83696	WGS84	BC11640	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.555	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11640_Mgig_Bot2012_COI	S12-06	1	BC11640_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11640	Sample	BC11640	targeted	Present	Sample	Mgig_Bot2012_COI	S12-06	COI	45	BC11640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.50982
BC11644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80897	WGS84	BC11644	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.686	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S12	20/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11644_Mgig_Bot2012_COI	S12-10	1	BC11644_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11644	Sample	BC11644	targeted	Present	Sample	Mgig_Bot2012_COI	S12-10	COI	45	BC11644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S12-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.51763
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.962	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11647_Aamu_Bax2006_COI	T17-01	1	BC11647_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Present	Sample	Aamu_Bax2006_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	GCACAACCGGGATCTTTACTTC	147.53883
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11647_Pcan_Bot2011_IGS	T17-01	1	BC11647_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-01	IGS	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	CGTAATCCTCAGTACTGGCTA	147.53883
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11647_Sspa_Oph2007_COI	T17-01	1	BC11647_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	GAAGGCCTTCCGATGATC	147.53883
BC11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9626	WGS84	BC11647	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11647_Upin_Bot2011_COI	T17-01	1	BC11647_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11647	Sample	BC11647	targeted	Absent	Sample	Upin_Bot2011_COI	T17-01	COI	45	BC11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-01	CTTTAATTACAGCGTTTTTATTGTTGT	147.53883
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11648_Cjap_Sim2018_COI	T17-03	1	BC11648_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51982
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.982	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	11/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11648_Mgig_Bot2012_COI	T17-03	1	BC11648_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Present	Sample	Mgig_Bot2012_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.51982
BC11648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.9642	WGS84	BC11648	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11648_Mjap_Gib2022_COI	T17-03	1	BC11648_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11648	Sample	BC11648	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-03	COI	45	BC11648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-03	AATTTAATTGTGACTAGGCATGGTCTG	147.51982
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	29.48	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11649_Cmae_Bot2011_COI	T17-04	1	BC11649_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Present	Sample	Cmae_Bot2011_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	ATGAACAGTCTATCCTCCTTTAG	147.52798
BC11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.95973	WGS84	BC11649	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.944	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	11/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11649_Mros_Gib2022_COI	T17-04	1	BC11649_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11649	Sample	BC11649	targeted	Present	Sample	Mros_Gib2022_COI	T17-04	COI	45	BC11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-04	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.52798
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11650_Aamu_Bax2006_COI	T17-05	1	BC11650_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Absent	Sample	Aamu_Bax2006_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	GCACAACCGGGATCTTTACTTC	147.47356
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11650_Pcan_Bot2011_IGS	T17-05	1	BC11650_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-05	IGS	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	CGTAATCCTCAGTACTGGCTA	147.47356
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11650_Sspa_Oph2007_COI	T17-05	1	BC11650_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	GAAGGCCTTCCGATGATC	147.47356
BC11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.79976	WGS84	BC11650	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11650_Upin_Bot2011_COI	T17-05	1	BC11650_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11650	Sample	BC11650	targeted	Absent	Sample	Upin_Bot2011_COI	T17-05	COI	45	BC11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-05	CTTTAATTACAGCGTTTTTATTGTTGT	147.47356
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11651_Cjap_Sim2018_COI	T17-07	1	BC11651_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Absent	Sample	Cjap_Sim2018_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.50293
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.159	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T17	22/11/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11651_Mgig_Bot2012_COI	T17-07	1	BC11651_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Present	Sample	Mgig_Bot2012_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.50293
BC11651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81025	WGS84	BC11651	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11651_Mjap_Gib2022_COI	T17-07	1	BC11651_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11651	Sample	BC11651	targeted	Absent	Sample	Mjap_Gib2022_COI	T17-07	COI	45	BC11651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-07	AATTTAATTGTGACTAGGCATGGTCTG	147.50293
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.483	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11652_Cmae_Bot2011_COI	T17-08	1	BC11652_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Present	Sample	Cmae_Bot2011_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	ATGAACAGTCTATCCTCCTTTAG	147.52213
BC11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8039	WGS84	BC11652	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.65	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11652_Mros_Gib2022_COI	T17-08	1	BC11652_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11652	Sample	BC11652	targeted	Present	Sample	Mros_Gib2022_COI	T17-08	COI	45	BC11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.52213
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11654_Aamu_Bax2006_COI	T17-06	1	BC11654_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Absent	Sample	Aamu_Bax2006_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	GCACAACCGGGATCTTTACTTC	147.4865
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11654_Pcan_Bot2011_IGS	T17-06	1	BC11654_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Absent	Sample	Pcan_Bot2011_IGS	T17-06	IGS	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	CGTAATCCTCAGTACTGGCTA	147.4865
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11654_Sspa_Oph2007_COI	T17-06	1	BC11654_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Absent	Sample	Sspa_Oph2007_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	GAAGGCCTTCCGATGATC	147.4865
BC11654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.81178	WGS84	BC11654	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T17	22/11/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11654_Upin_Bot2011_COI	T17-06	1	BC11654_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11654	Sample	BC11654	targeted	Absent	Sample	Upin_Bot2011_COI	T17-06	COI	45	BC11654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T17-06	CTTTAATTACAGCGTTTTTATTGTTGT	147.4865
BC11659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.78365	WGS84	BC11659	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11659_Mgig_Bot2012_COI	S9-03	1	BC11659_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11659	Sample	BC11659	targeted	Absent	Sample	Mgig_Bot2012_COI	S9-03	COI	45	BC11659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.51601
BC11662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.8436	WGS84	BC11662	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11662_Mgig_Bot2012_COI	S9-06	1	BC11662_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11662	Sample	BC11662	targeted	Absent	Sample	Mgig_Bot2012_COI	S9-06	COI	45	BC11662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-06	TCTTATTCGTTGGAGACTTTATAACCCT	138.4974
BC11666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11877	-34.80886	WGS84	BC11666	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S09	9/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11666_Mgig_Bot2012_COI	S9-10	1	BC11666_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11666	Sample	BC11666	targeted	Absent	Sample	Mgig_Bot2012_COI	S9-10	COI	45	BC11666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S9-10	TCTTATTCGTTGGAGACTTTATAACCCT	138.51726
BC11667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96492	WGS84	BC11667	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.905	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11667_Cmae_Bot2011_COI	T18-09	1	BC11667_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11667	Sample	BC11667	targeted	Present	Sample	Cmae_Bot2011_COI	T18-09	COI	45	BC11667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-09	ATGAACAGTCTATCCTCCTTTAG	147.51981
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.411	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11672_Aamu_Bax2006_COI	T15-14	1	BC11672_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Present	Sample	Aamu_Bax2006_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	GCACAACCGGGATCTTTACTTC	147.51958
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11672_Pcan_Bot2011_IGS	T15-14	1	BC11672_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-14	IGS	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	CGTAATCCTCAGTACTGGCTA	147.51958
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11672_Sspa_Oph2007_COI	T15-14	1	BC11672_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	GAAGGCCTTCCGATGATC	147.51958
BC11672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96477	WGS84	BC11672	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.145	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	30/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11672_Upin_Bot2011_COI	T15-14	1	BC11672_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11672	Sample	BC11672	targeted	Present	Sample	Upin_Bot2011_COI	T15-14	COI	45	BC11672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.51958
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11673_Cjap_Sim2018_COI	T15-13	1	BC11673_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.3371
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11673_Mgig_Bot2012_COI	T15-13	1	BC11673_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	TCTTATTCGTTGGAGACTTTATAACCCT	147.3371
BC11673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88283	WGS84	BC11673	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11673_Mjap_Gib2022_COI	T15-13	1	BC11673_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11673	Sample	BC11673	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-13	COI	45	BC11673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-13	AATTTAATTGTGACTAGGCATGGTCTG	147.3371
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11674_Cmae_Bot2011_COI	T15-12	1	BC11674_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	ATGAACAGTCTATCCTCCTTTAG	147.33631
BC11674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88398	WGS84	BC11674	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11674_Mros_Gib2022_COI	T15-12	1	BC11674_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11674	Sample	BC11674	targeted	Absent	Sample	Mros_Gib2022_COI	T15-12	COI	45	BC11674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-12	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33631
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.897	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11676_Aamu_Bax2006_COI	T15-10	1	BC11676_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Present	Sample	Aamu_Bax2006_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	GCACAACCGGGATCTTTACTTC	147.34183
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11676_Pcan_Bot2011_IGS	T15-10	1	BC11676_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-10	IGS	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	CGTAATCCTCAGTACTGGCTA	147.34183
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11676_Sspa_Oph2007_COI	T15-10	1	BC11676_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	GAAGGCCTTCCGATGATC	147.34183
BC11676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87883	WGS84	BC11676	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11676_Upin_Bot2011_COI	T15-10	1	BC11676_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11676	Sample	BC11676	targeted	Absent	Sample	Upin_Bot2011_COI	T15-10	COI	45	BC11676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-10	CTTTAATTACAGCGTTTTTATTGTTGT	147.34183
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11677_Cjap_Sim2018_COI	T15-09	1	BC11677_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.34172
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.03	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11677_Mgig_Bot2012_COI	T15-09	1	BC11677_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Present	Sample	Mgig_Bot2012_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	TCTTATTCGTTGGAGACTTTATAACCCT	147.34172
BC11677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88116	WGS84	BC11677	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11677_Mjap_Gib2022_COI	T15-09	1	BC11677_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11677	Sample	BC11677	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-09	COI	45	BC11677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-09	AATTTAATTGTGACTAGGCATGGTCTG	147.34172
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11678_Cmae_Bot2011_COI	T15-08	1	BC11678_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	ATGAACAGTCTATCCTCCTTTAG	147.33654
BC11678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87268	WGS84	BC11678	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11678_Mros_Gib2022_COI	T15-08	1	BC11678_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11678	Sample	BC11678	targeted	Absent	Sample	Mros_Gib2022_COI	T15-08	COI	45	BC11678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-08	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33654
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11680_Cjap_Sim2018_COI	T15-06	1	BC11680_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30245
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	28/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11680_Mgig_Bot2012_COI	T15-06	1	BC11680_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	TCTTATTCGTTGGAGACTTTATAACCCT	147.30245
BC11680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82613	WGS84	BC11680	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11680_Mjap_Gib2022_COI	T15-06	1	BC11680_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11680	Sample	BC11680	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-06	COI	45	BC11680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-06	AATTTAATTGTGACTAGGCATGGTCTG	147.30245
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11681_Cmae_Bot2011_COI	T15-05	1	BC11681_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	ATGAACAGTCTATCCTCCTTTAG	147.30244
BC11681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82935	WGS84	BC11681	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	28/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11681_Mros_Gib2022_COI	T15-05	1	BC11681_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11681	Sample	BC11681	targeted	Absent	Sample	Mros_Gib2022_COI	T15-05	COI	45	BC11681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-05	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.30244
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.638	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11683_Aamu_Bax2006_COI	T15-03	1	BC11683_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Present	Sample	Aamu_Bax2006_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	GCACAACCGGGATCTTTACTTC	147.87269
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11683_Pcan_Bot2011_IGS	T15-03	1	BC11683_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Absent	Sample	Pcan_Bot2011_IGS	T15-03	IGS	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	CGTAATCCTCAGTACTGGCTA	147.87269
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11683_Sspa_Oph2007_COI	T15-03	1	BC11683_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Absent	Sample	Sspa_Oph2007_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	GAAGGCCTTCCGATGATC	147.87269
BC11683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85567	WGS84	BC11683	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.448	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11683_Upin_Bot2011_COI	T15-03	1	BC11683_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11683	Sample	BC11683	targeted	Present	Sample	Upin_Bot2011_COI	T15-03	COI	45	BC11683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.87269
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11684_Cjap_Sim2018_COI	T15-02	1	BC11684_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Absent	Sample	Cjap_Sim2018_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87397
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T15	24/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11684_Mgig_Bot2012_COI	T15-02	1	BC11684_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Absent	Sample	Mgig_Bot2012_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	TCTTATTCGTTGGAGACTTTATAACCCT	147.87397
BC11684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85093	WGS84	BC11684	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11684_Mjap_Gib2022_COI	T15-02	1	BC11684_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11684	Sample	BC11684	targeted	Absent	Sample	Mjap_Gib2022_COI	T15-02	COI	45	BC11684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-02	AATTTAATTGTGACTAGGCATGGTCTG	147.87397
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11685_Cmae_Bot2011_COI	T15-01	1	BC11685_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Absent	Sample	Cmae_Bot2011_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	ATGAACAGTCTATCCTCCTTTAG	147.87463
BC11685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.84743	WGS84	BC11685	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T15	24/08/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11685_Mros_Gib2022_COI	T15-01	1	BC11685_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11685	Sample	BC11685	targeted	Absent	Sample	Mros_Gib2022_COI	T15-01	COI	45	BC11685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T15-01	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87463
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.574	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11687_Aamu_Bax2006_COI	T14-15	1	BC11687_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Present	Sample	Aamu_Bax2006_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	GCACAACCGGGATCTTTACTTC	147.5197
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11687_Pcan_Bot2011_IGS	T14-15	1	BC11687_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-15	IGS	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	CGTAATCCTCAGTACTGGCTA	147.5197
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11687_Sspa_Oph2007_COI	T14-15	1	BC11687_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	GAAGGCCTTCCGATGATC	147.5197
BC11687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96507	WGS84	BC11687	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11687_Upin_Bot2011_COI	T14-15	1	BC11687_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11687	Sample	BC11687	targeted	Absent	Sample	Upin_Bot2011_COI	T14-15	COI	45	BC11687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-15	CTTTAATTACAGCGTTTTTATTGTTGT	147.5197
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11688_Cjap_Sim2018_COI	T14-14	1	BC11688_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.53562
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.459	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	8/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11688_Mgig_Bot2012_COI	T14-14	1	BC11688_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Present	Sample	Mgig_Bot2012_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	TCTTATTCGTTGGAGACTTTATAACCCT	147.53562
BC11688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.96309	WGS84	BC11688	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11688_Mjap_Gib2022_COI	T14-14	1	BC11688_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11688	Sample	BC11688	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-14	COI	45	BC11688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-14	AATTTAATTGTGACTAGGCATGGTCTG	147.53562
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11689_Cmae_Bot2011_COI	T14-13	1	BC11689_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Cmae_Bot2011_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	ATGAACAGTCTATCCTCCTTTAG	147.33753
BC11689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.8741	WGS84	BC11689	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11689_Mros_Gib2022_COI	T14-13	1	BC11689_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11689	Sample	BC11689	targeted	Absent	Sample	Mros_Gib2022_COI	T14-13	COI	45	BC11689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-13	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33753
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.199	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11690_Aamu_Bax2006_COI	T14-12	1	BC11690_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Present	Sample	Aamu_Bax2006_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	GCACAACCGGGATCTTTACTTC	147.34184
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11690_Pcan_Bot2011_IGS	T14-12	1	BC11690_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-12	IGS	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	CGTAATCCTCAGTACTGGCTA	147.34184
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11690_Sspa_Oph2007_COI	T14-12	1	BC11690_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	GAAGGCCTTCCGATGATC	147.34184
BC11690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.87889	WGS84	BC11690	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11690_Upin_Bot2011_COI	T14-12	1	BC11690_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11690	Sample	BC11690	targeted	Absent	Sample	Upin_Bot2011_COI	T14-12	COI	45	BC11690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-12	CTTTAATTACAGCGTTTTTATTGTTGT	147.34184
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11691_Cjap_Sim2018_COI	T14-16	1	BC11691_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52389
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.362	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	8/02/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11691_Mgig_Bot2012_COI	T14-16	1	BC11691_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Present	Sample	Mgig_Bot2012_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	TCTTATTCGTTGGAGACTTTATAACCCT	147.52389
BC11691	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.963	WGS84	BC11691	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	8/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11691_Mjap_Gib2022_COI	T14-16	1	BC11691_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11691	Sample	BC11691	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-16	COI	45	BC11691	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-16	AATTTAATTGTGACTAGGCATGGTCTG	147.52389
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11692_Cmae_Bot2011_COI	T14-10	1	BC11692_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Cmae_Bot2011_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	ATGAACAGTCTATCCTCCTTTAG	147.33662
BC11692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88286	WGS84	BC11692	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11692_Mros_Gib2022_COI	T14-10	1	BC11692_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11692	Sample	BC11692	targeted	Absent	Sample	Mros_Gib2022_COI	T14-10	COI	45	BC11692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-10	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.33662
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.952	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11694_Aamu_Bax2006_COI	T14-08	1	BC11694_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Present	Sample	Aamu_Bax2006_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	GCACAACCGGGATCTTTACTTC	147.33681
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11694_Pcan_Bot2011_IGS	T14-08	1	BC11694_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-08	IGS	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	CGTAATCCTCAGTACTGGCTA	147.33681
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11694_Sspa_Oph2007_COI	T14-08	1	BC11694_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	GAAGGCCTTCCGATGATC	147.33681
BC11694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88578	WGS84	BC11694	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11694_Upin_Bot2011_COI	T14-08	1	BC11694_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11694	Sample	BC11694	targeted	Absent	Sample	Upin_Bot2011_COI	T14-08	COI	45	BC11694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-08	CTTTAATTACAGCGTTTTTATTGTTGT	147.33681
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11695_Cjap_Sim2018_COI	T14-07	1	BC11695_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.30768
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	31/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11695_Mgig_Bot2012_COI	T14-07	1	BC11695_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	TCTTATTCGTTGGAGACTTTATAACCCT	147.30768
BC11695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82524	WGS84	BC11695	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11695_Mjap_Gib2022_COI	T14-07	1	BC11695_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11695	Sample	BC11695	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-07	COI	45	BC11695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-07	AATTTAATTGTGACTAGGCATGGTCTG	147.30768
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11696_Cmae_Bot2011_COI	T14-06	1	BC11696_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Cmae_Bot2011_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	ATGAACAGTCTATCCTCCTTTAG	147.3019
BC11696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.82683	WGS84	BC11696	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	31/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11696_Mros_Gib2022_COI	T14-06	1	BC11696_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11696	Sample	BC11696	targeted	Absent	Sample	Mros_Gib2022_COI	T14-06	COI	45	BC11696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-06	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.3019
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.84	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC11698_Aamu_Bax2006_COI	T14-04	1	BC11698_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Present	Sample	Aamu_Bax2006_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	GCACAACCGGGATCTTTACTTC	147.81605
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC11698_Pcan_Bot2011_IGS	T14-04	1	BC11698_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Absent	Sample	Pcan_Bot2011_IGS	T14-04	IGS	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	CGTAATCCTCAGTACTGGCTA	147.81605
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC11698_Sspa_Oph2007_COI	T14-04	1	BC11698_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Absent	Sample	Sspa_Oph2007_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	GAAGGCCTTCCGATGATC	147.81605
BC11698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.88932	WGS84	BC11698	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.692	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC11698_Upin_Bot2011_COI	T14-04	1	BC11698_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11698	Sample	BC11698	targeted	Present	Sample	Upin_Bot2011_COI	T14-04	COI	45	BC11698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-04	CTTTAATTACAGCGTTTTTATTGTTGT	147.81605
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC11699_Cjap_Sim2018_COI	T14-03	1	BC11699_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Absent	Sample	Cjap_Sim2018_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87279
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T14	26/07/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC11699_Mgig_Bot2012_COI	T14-03	1	BC11699_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Absent	Sample	Mgig_Bot2012_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	TCTTATTCGTTGGAGACTTTATAACCCT	147.87279
BC11699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85553	WGS84	BC11699	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC11699_Mjap_Gib2022_COI	T14-03	1	BC11699_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11699	Sample	BC11699	targeted	Absent	Sample	Mjap_Gib2022_COI	T14-03	COI	45	BC11699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-03	AATTTAATTGTGACTAGGCATGGTCTG	147.87279
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.791	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC11700_Cmae_Bot2011_COI	T14-02	1	BC11700_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Present	Sample	Cmae_Bot2011_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	ATGAACAGTCTATCCTCCTTTAG	147.87373
BC11700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11878	-42.85117	WGS84	BC11700	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	Oyster_CRC-P_surveys-T14	26/07/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC11700_Mros_Gib2022_COI	T14-02	1	BC11700_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC11700	Sample	BC11700	targeted	Absent	Sample	Mros_Gib2022_COI	T14-02	COI	45	BC11700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T14-02	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87373
BC15784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88221	WGS84	BC15784	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15784_Cmae_Bot2011_COI	HA1	0	BC15784_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15784	Sample	BC15784	targeted	Absent	Sample	Cmae_Bot2011_COI	HA1	COI	45	BC15784	1.00E-05	Nil	Australia	HA1	ATGAACAGTCTATCCTCCTTTAG	147.34465
BC15786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88289	WGS84	BC15786	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.277	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15786_Aamu_Bax2006_COI	HA13	0	BC15786_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15786	Sample	BC15786	targeted	Present	Sample	Aamu_Bax2006_COI	HA13	COI	45	BC15786	1.00E-05	Nil	Australia	HA13	GCACAACCGGGATCTTTACTTC	147.33818
BC15786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88289	WGS84	BC15786	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15786_Sspa_Oph2007_COI	HA13	0	BC15786_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15786	Sample	BC15786	targeted	Absent	Sample	Sspa_Oph2007_COI	HA13	COI	45	BC15786	1.00E-05	Nil	Australia	HA13	GAAGGCCTTCCGATGATC	147.33818
BC15786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88289	WGS84	BC15786	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15786_Upin_Bot2011_COI	HA13	0	BC15786_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15786	Sample	BC15786	targeted	Absent	Sample	Upin_Bot2011_COI	HA13	COI	45	BC15786	1.00E-05	Nil	Australia	HA13	CTTTAATTACAGCGTTTTTATTGTTGT	147.33818
BC15787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84498	WGS84	BC15787	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15787_Mgig_Bot2012_COI	HA19	0	BC15787_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15787	Sample	BC15787	targeted	Absent	Sample	Mgig_Bot2012_COI	HA19	COI	45	BC15787	1.00E-05	Nil	Australia	HA19	TCTTATTCGTTGGAGACTTTATAACCCT	147.32942
BC15788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84393	WGS84	BC15788	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.637	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15788_Cmae_Bot2011_COI	HA21	0	BC15788_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15788	Sample	BC15788	targeted	Present	Sample	Cmae_Bot2011_COI	HA21	COI	45	BC15788	1.00E-05	Nil	Australia	HA21	ATGAACAGTCTATCCTCCTTTAG	147.32811
BC15790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88608	WGS84	BC15790	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Winter	8/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15790_Mgig_Bot2012_COI	HA3	0	BC15790_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15790	Sample	BC15790	targeted	Absent	Sample	Mgig_Bot2012_COI	HA3	COI	45	BC15790	1.00E-05	Nil	Australia	HA3	TCTTATTCGTTGGAGACTTTATAACCCT	147.33912
BC15791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18983	WGS84	BC15791	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15791_Cmae_Bot2011_COI	DD2	0	BC15791_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15791	Sample	BC15791	targeted	Absent	Sample	Cmae_Bot2011_COI	DD2	COI	45	BC15791	1.00E-05	Nil	Australia	DD2	ATGAACAGTCTATCCTCCTTTAG	146.36787
BC15793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18467	WGS84	BC15793	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15793_Aamu_Bax2006_COI	DD11	0	BC15793_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15793	Sample	BC15793	targeted	Absent	Sample	Aamu_Bax2006_COI	DD11	COI	45	BC15793	1.00E-05	Nil	Australia	DD11	GCACAACCGGGATCTTTACTTC	146.36495
BC15793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18467	WGS84	BC15793	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15793_Sspa_Oph2007_COI	DD11	0	BC15793_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15793	Sample	BC15793	targeted	Absent	Sample	Sspa_Oph2007_COI	DD11	COI	45	BC15793	1.00E-05	Nil	Australia	DD11	GAAGGCCTTCCGATGATC	146.36495
BC15793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18467	WGS84	BC15793	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15793_Upin_Bot2011_COI	DD11	0	BC15793_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15793	Sample	BC15793	targeted	Absent	Sample	Upin_Bot2011_COI	DD11	COI	45	BC15793	1.00E-05	Nil	Australia	DD11	CTTTAATTACAGCGTTTTTATTGTTGT	146.36495
BC15794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-41.18373	WGS84	BC15794	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	2018-19_Molecular_surveys-Devonport_Autumn	6/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15794_Mgig_Bot2012_COI	DD14	0	BC15794_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15794	Sample	BC15794	targeted	Absent	Sample	Mgig_Bot2012_COI	DD14	COI	45	BC15794	1.00E-05	Nil	Australia	DD14	TCTTATTCGTTGGAGACTTTATAACCCT	146.3646
BC15795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84704	WGS84	BC15795	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15795_Cmae_Bot2011_COI	MB27	0	BC15795_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15795	Sample	BC15795	targeted	Absent	Sample	Cmae_Bot2011_COI	MB27	COI	45	BC15795	1.00E-05	Nil	Australia	MB27	ATGAACAGTCTATCCTCCTTTAG	144.9319
BC15797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84189	WGS84	BC15797	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.21	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15797_Aamu_Bax2006_COI	MB21	0	BC15797_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15797	Sample	BC15797	targeted	Present	Sample	Aamu_Bax2006_COI	MB21	COI	45	BC15797	1.00E-05	Nil	Australia	MB21	GCACAACCGGGATCTTTACTTC	144.92719
BC15797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84189	WGS84	BC15797	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.45	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15797_Sspa_Oph2007_COI	MB21	0	BC15797_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15797	Sample	BC15797	targeted	Present	Sample	Sspa_Oph2007_COI	MB21	COI	45	BC15797	1.00E-05	Nil	Australia	MB21	GAAGGCCTTCCGATGATC	144.92719
BC15797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84189	WGS84	BC15797	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.055	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15797_Upin_Bot2011_COI	MB21	0	BC15797_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15797	Sample	BC15797	targeted	Present	Sample	Upin_Bot2011_COI	MB21	COI	45	BC15797	1.00E-05	Nil	Australia	MB21	CTTTAATTACAGCGTTTTTATTGTTGT	144.92719
BC15798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84384	WGS84	BC15798	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15798_Mgig_Bot2012_COI	MB19	0	BC15798_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15798	Sample	BC15798	targeted	Absent	Sample	Mgig_Bot2012_COI	MB19	COI	45	BC15798	1.00E-05	Nil	Australia	MB19	TCTTATTCGTTGGAGACTTTATAACCCT	144.92641
BC15799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85028	WGS84	BC15799	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15799_Cmae_Bot2011_COI	MB17	0	BC15799_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15799	Sample	BC15799	targeted	Absent	Sample	Cmae_Bot2011_COI	MB17	COI	45	BC15799	1.00E-05	Nil	Australia	MB17	ATGAACAGTCTATCCTCCTTTAG	144.91393
BC15801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82417	WGS84	BC15801	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15801_Aamu_Bax2006_COI	MB123	0	BC15801_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15801	Sample	BC15801	targeted	Absent	Sample	Aamu_Bax2006_COI	MB123	COI	45	BC15801	1.00E-05	Nil	Australia	MB123	GCACAACCGGGATCTTTACTTC	144.9012
BC15801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82417	WGS84	BC15801	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.565	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15801_Sspa_Oph2007_COI	MB123	0	BC15801_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15801	Sample	BC15801	targeted	Present	Sample	Sspa_Oph2007_COI	MB123	COI	45	BC15801	1.00E-05	Nil	Australia	MB123	GAAGGCCTTCCGATGATC	144.9012
BC15801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82417	WGS84	BC15801	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15801_Upin_Bot2011_COI	MB123	0	BC15801_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15801	Sample	BC15801	targeted	Absent	Sample	Upin_Bot2011_COI	MB123	COI	45	BC15801	1.00E-05	Nil	Australia	MB123	CTTTAATTACAGCGTTTTTATTGTTGT	144.9012
BC15802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82346	WGS84	BC15802	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.687	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15802_Mgig_Bot2012_COI	MB122	0	BC15802_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15802	Sample	BC15802	targeted	Present	Sample	Mgig_Bot2012_COI	MB122	COI	45	BC15802	1.00E-05	Nil	Australia	MB122	TCTTATTCGTTGGAGACTTTATAACCCT	144.90165
BC15803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82012	WGS84	BC15803	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15803_Cmae_Bot2011_COI	MB118	0	BC15803_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15803	Sample	BC15803	targeted	Absent	Sample	Cmae_Bot2011_COI	MB118	COI	45	BC15803	1.00E-05	Nil	Australia	MB118	ATGAACAGTCTATCCTCCTTTAG	144.90557
BC15805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81639	WGS84	BC15805	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	35.078	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15805_Aamu_Bax2006_COI	MB114	0	BC15805_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15805	Sample	BC15805	targeted	Present	Sample	Aamu_Bax2006_COI	MB114	COI	45	BC15805	1.00E-05	Nil	Australia	MB114	GCACAACCGGGATCTTTACTTC	144.90628
BC15805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81639	WGS84	BC15805	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.971	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15805_Sspa_Oph2007_COI	MB114	0	BC15805_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15805	Sample	BC15805	targeted	Present	Sample	Sspa_Oph2007_COI	MB114	COI	45	BC15805	1.00E-05	Nil	Australia	MB114	GAAGGCCTTCCGATGATC	144.90628
BC15805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81639	WGS84	BC15805	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15805_Upin_Bot2011_COI	MB114	0	BC15805_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15805	Sample	BC15805	targeted	Absent	Sample	Upin_Bot2011_COI	MB114	COI	45	BC15805	1.00E-05	Nil	Australia	MB114	CTTTAATTACAGCGTTTTTATTGTTGT	144.90628
BC15806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81908	WGS84	BC15806	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.944	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15806_Mgig_Bot2012_COI	MB111	0	BC15806_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15806	Sample	BC15806	targeted	Present	Sample	Mgig_Bot2012_COI	MB111	COI	45	BC15806	1.00E-05	Nil	Australia	MB111	TCTTATTCGTTGGAGACTTTATAACCCT	144.90711
BC15807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81996	WGS84	BC15807	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15807_Cmae_Bot2011_COI	MB109	0	BC15807_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15807	Sample	BC15807	targeted	Absent	Sample	Cmae_Bot2011_COI	MB109	COI	45	BC15807	1.00E-05	Nil	Australia	MB109	ATGAACAGTCTATCCTCCTTTAG	144.90884
BC15809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81839	WGS84	BC15809	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15809_Aamu_Bax2006_COI	MB106	0	BC15809_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15809	Sample	BC15809	targeted	Absent	Sample	Aamu_Bax2006_COI	MB106	COI	45	BC15809	1.00E-05	Nil	Australia	MB106	GCACAACCGGGATCTTTACTTC	144.91417
BC15809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81839	WGS84	BC15809	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.038	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15809_Sspa_Oph2007_COI	MB106	0	BC15809_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15809	Sample	BC15809	targeted	Present	Sample	Sspa_Oph2007_COI	MB106	COI	45	BC15809	1.00E-05	Nil	Australia	MB106	GAAGGCCTTCCGATGATC	144.91417
BC15809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81839	WGS84	BC15809	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/12/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15809_Upin_Bot2011_COI	MB106	0	BC15809_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15809	Sample	BC15809	targeted	Absent	Sample	Upin_Bot2011_COI	MB106	COI	45	BC15809	1.00E-05	Nil	Australia	MB106	CTTTAATTACAGCGTTTTTATTGTTGT	144.91417
BC15810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81528	WGS84	BC15810	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15810_Cmae_Bot2011_COI	MB103	0	BC15810_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15810	Sample	BC15810	targeted	Absent	Sample	Cmae_Bot2011_COI	MB103	COI	45	BC15810	1.00E-05	Nil	Australia	MB103	ATGAACAGTCTATCCTCCTTTAG	144.91289
BC15812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.83951	WGS84	BC15812	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	32.545	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15812_Aamu_Bax2006_COI	MB1	0	BC15812_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15812	Sample	BC15812	targeted	Present	Sample	Aamu_Bax2006_COI	MB1	COI	45	BC15812	1.00E-05	Nil	Australia	MB1	GCACAACCGGGATCTTTACTTC	144.90387
BC15812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.83951	WGS84	BC15812	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.604	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15812_Sspa_Oph2007_COI	MB1	0	BC15812_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15812	Sample	BC15812	targeted	Present	Sample	Sspa_Oph2007_COI	MB1	COI	45	BC15812	1.00E-05	Nil	Australia	MB1	GAAGGCCTTCCGATGATC	144.90387
BC15812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.83951	WGS84	BC15812	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.394	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15812_Upin_Bot2011_COI	MB1	0	BC15812_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15812	Sample	BC15812	targeted	Present	Sample	Upin_Bot2011_COI	MB1	COI	45	BC15812	1.00E-05	Nil	Australia	MB1	CTTTAATTACAGCGTTTTTATTGTTGT	144.90387
BC15816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81807	WGS84	BC15816	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.68	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15816_Aamu_Bax2006_COI	MA93	0	BC15816_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15816	Sample	BC15816	targeted	Present	Sample	Aamu_Bax2006_COI	MA93	COI	45	BC15816	1.00E-05	Nil	Australia	MA93	GCACAACCGGGATCTTTACTTC	144.91519
BC15816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81807	WGS84	BC15816	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.943	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15816_Sspa_Oph2007_COI	MA93	0	BC15816_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15816	Sample	BC15816	targeted	Present	Sample	Sspa_Oph2007_COI	MA93	COI	45	BC15816	1.00E-05	Nil	Australia	MA93	GAAGGCCTTCCGATGATC	144.91519
BC15816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81807	WGS84	BC15816	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15816_Upin_Bot2011_COI	MA93	0	BC15816_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15816	Sample	BC15816	targeted	Absent	Sample	Upin_Bot2011_COI	MA93	COI	45	BC15816	1.00E-05	Nil	Australia	MA93	CTTTAATTACAGCGTTTTTATTGTTGT	144.91519
BC15817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81763	WGS84	BC15817	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.884	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15817_Mgig_Bot2012_COI	MA92	0	BC15817_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15817	Sample	BC15817	targeted	Present	Sample	Mgig_Bot2012_COI	MA92	COI	45	BC15817	1.00E-05	Nil	Australia	MA92	TCTTATTCGTTGGAGACTTTATAACCCT	144.92
BC15818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85808	WGS84	BC15818	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15818_Cmae_Bot2011_COI	MA9	0	BC15818_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15818	Sample	BC15818	targeted	Absent	Sample	Cmae_Bot2011_COI	MA9	COI	45	BC15818	1.00E-05	Nil	Australia	MA9	ATGAACAGTCTATCCTCCTTTAG	144.91
BC15820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85984	WGS84	BC15820	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15820_Mgig_Bot2012_COI	MA86	0	BC15820_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15820	Sample	BC15820	targeted	Absent	Sample	Mgig_Bot2012_COI	MA86	COI	45	BC15820	1.00E-05	Nil	Australia	MA86	TCTTATTCGTTGGAGACTTTATAACCCT	144.91506
BC15821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85992	WGS84	BC15821	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.268	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15821_Cmae_Bot2011_COI	MA84	0	BC15821_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15821	Sample	BC15821	targeted	Present	Sample	Cmae_Bot2011_COI	MA84	COI	45	BC15821	1.00E-05	Nil	Australia	MA84	ATGAACAGTCTATCCTCCTTTAG	144.91472
BC15823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86202	WGS84	BC15823	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.507	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15823_Aamu_Bax2006_COI	MA82	0	BC15823_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15823	Sample	BC15823	targeted	Present	Sample	Aamu_Bax2006_COI	MA82	COI	45	BC15823	1.00E-05	Nil	Australia	MA82	GCACAACCGGGATCTTTACTTC	144.91645
BC15823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86202	WGS84	BC15823	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.21	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15823_Sspa_Oph2007_COI	MA82	0	BC15823_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15823	Sample	BC15823	targeted	Present	Sample	Sspa_Oph2007_COI	MA82	COI	45	BC15823	1.00E-05	Nil	Australia	MA82	GAAGGCCTTCCGATGATC	144.91645
BC15823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86202	WGS84	BC15823	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.606	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15823_Upin_Bot2011_COI	MA82	0	BC15823_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15823	Sample	BC15823	targeted	Present	Sample	Upin_Bot2011_COI	MA82	COI	45	BC15823	1.00E-05	Nil	Australia	MA82	CTTTAATTACAGCGTTTTTATTGTTGT	144.91645
BC15824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86328	WGS84	BC15824	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15824_Mgig_Bot2012_COI	MA74	0	BC15824_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15824	Sample	BC15824	targeted	Absent	Sample	Mgig_Bot2012_COI	MA74	COI	45	BC15824	1.00E-05	Nil	Australia	MA74	TCTTATTCGTTGGAGACTTTATAACCCT	144.91845
BC15825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86447	WGS84	BC15825	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15825_Cmae_Bot2011_COI	MA73	0	BC15825_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15825	Sample	BC15825	targeted	Absent	Sample	Cmae_Bot2011_COI	MA73	COI	45	BC15825	1.00E-05	Nil	Australia	MA73	ATGAACAGTCTATCCTCCTTTAG	144.91705
BC15827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86208	WGS84	BC15827	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.999	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15827_Aamu_Bax2006_COI	MA67	0	BC15827_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15827	Sample	BC15827	targeted	Present	Sample	Aamu_Bax2006_COI	MA67	COI	45	BC15827	1.00E-05	Nil	Australia	MA67	GCACAACCGGGATCTTTACTTC	144.91525
BC15827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86208	WGS84	BC15827	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.444	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15827_Sspa_Oph2007_COI	MA67	0	BC15827_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15827	Sample	BC15827	targeted	Present	Sample	Sspa_Oph2007_COI	MA67	COI	45	BC15827	1.00E-05	Nil	Australia	MA67	GAAGGCCTTCCGATGATC	144.91525
BC15827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86208	WGS84	BC15827	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.243	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15827_Upin_Bot2011_COI	MA67	0	BC15827_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15827	Sample	BC15827	targeted	Present	Sample	Upin_Bot2011_COI	MA67	COI	45	BC15827	1.00E-05	Nil	Australia	MA67	CTTTAATTACAGCGTTTTTATTGTTGT	144.91525
BC15830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8263	WGS84	BC15830	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.785	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15830_Aamu_Bax2006_COI	MA58	0	BC15830_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15830	Sample	BC15830	targeted	Present	Sample	Aamu_Bax2006_COI	MA58	COI	45	BC15830	1.00E-05	Nil	Australia	MA58	GCACAACCGGGATCTTTACTTC	144.90263
BC15830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8263	WGS84	BC15830	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.709	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15830_Sspa_Oph2007_COI	MA58	0	BC15830_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15830	Sample	BC15830	targeted	Present	Sample	Sspa_Oph2007_COI	MA58	COI	45	BC15830	1.00E-05	Nil	Australia	MA58	GAAGGCCTTCCGATGATC	144.90263
BC15830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8263	WGS84	BC15830	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15830_Upin_Bot2011_COI	MA58	0	BC15830_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15830	Sample	BC15830	targeted	Absent	Sample	Upin_Bot2011_COI	MA58	COI	45	BC15830	1.00E-05	Nil	Australia	MA58	CTTTAATTACAGCGTTTTTATTGTTGT	144.90263
BC15832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8201	WGS84	BC15832	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15832_Cmae_Bot2011_COI	MA56	0	BC15832_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15832	Sample	BC15832	targeted	Absent	Sample	Cmae_Bot2011_COI	MA56	COI	45	BC15832	1.00E-05	Nil	Australia	MA56	ATGAACAGTCTATCCTCCTTTAG	144.91851
BC15834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81754	WGS84	BC15834	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	22.228	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15834_Aamu_Bax2006_COI	MA46	0	BC15834_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15834	Sample	BC15834	targeted	Present	Sample	Aamu_Bax2006_COI	MA46	COI	45	BC15834	1.00E-05	Nil	Australia	MA46	GCACAACCGGGATCTTTACTTC	144.92417
BC15834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81754	WGS84	BC15834	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.094	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15834_Sspa_Oph2007_COI	MA46	0	BC15834_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15834	Sample	BC15834	targeted	Present	Sample	Sspa_Oph2007_COI	MA46	COI	45	BC15834	1.00E-05	Nil	Australia	MA46	GAAGGCCTTCCGATGATC	144.92417
BC15834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81754	WGS84	BC15834	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15834_Upin_Bot2011_COI	MA46	0	BC15834_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15834	Sample	BC15834	targeted	Absent	Sample	Upin_Bot2011_COI	MA46	COI	45	BC15834	1.00E-05	Nil	Australia	MA46	CTTTAATTACAGCGTTTTTATTGTTGT	144.92417
BC15835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85732	WGS84	BC15835	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15835_Mgig_Bot2012_COI	MA44	0	BC15835_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15835	Sample	BC15835	targeted	Absent	Sample	Mgig_Bot2012_COI	MA44	COI	45	BC15835	1.00E-05	Nil	Australia	MA44	TCTTATTCGTTGGAGACTTTATAACCCT	144.91012
BC15836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8645	WGS84	BC15836	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15836_Cmae_Bot2011_COI	MA42	0	BC15836_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15836	Sample	BC15836	targeted	Absent	Sample	Cmae_Bot2011_COI	MA42	COI	45	BC15836	1.00E-05	Nil	Australia	MA42	ATGAACAGTCTATCCTCCTTTAG	144.9176
BC15838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84279	WGS84	BC15838	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.742	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15838_Aamu_Bax2006_COI	MA37	0	BC15838_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15838	Sample	BC15838	targeted	Present	Sample	Aamu_Bax2006_COI	MA37	COI	45	BC15838	1.00E-05	Nil	Australia	MA37	GCACAACCGGGATCTTTACTTC	144.90429
BC15838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84279	WGS84	BC15838	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.095	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15838_Sspa_Oph2007_COI	MA37	0	BC15838_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15838	Sample	BC15838	targeted	Present	Sample	Sspa_Oph2007_COI	MA37	COI	45	BC15838	1.00E-05	Nil	Australia	MA37	GAAGGCCTTCCGATGATC	144.90429
BC15838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84279	WGS84	BC15838	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.339	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15838_Upin_Bot2011_COI	MA37	0	BC15838_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15838	Sample	BC15838	targeted	Present	Sample	Upin_Bot2011_COI	MA37	COI	45	BC15838	1.00E-05	Nil	Australia	MA37	CTTTAATTACAGCGTTTTTATTGTTGT	144.90429
BC15839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84415	WGS84	BC15839	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15839_Mgig_Bot2012_COI	MA36	0	BC15839_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15839	Sample	BC15839	targeted	Absent	Sample	Mgig_Bot2012_COI	MA36	COI	45	BC15839	1.00E-05	Nil	Australia	MA36	TCTTATTCGTTGGAGACTTTATAACCCT	144.90481
BC15841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84667	WGS84	BC15841	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.489	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15841_Aamu_Bax2006_COI	MA31	0	BC15841_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15841	Sample	BC15841	targeted	Present	Sample	Aamu_Bax2006_COI	MA31	COI	45	BC15841	1.00E-05	Nil	Australia	MA31	GCACAACCGGGATCTTTACTTC	144.90582
BC15841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84667	WGS84	BC15841	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.027	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15841_Sspa_Oph2007_COI	MA31	0	BC15841_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15841	Sample	BC15841	targeted	Present	Sample	Sspa_Oph2007_COI	MA31	COI	45	BC15841	1.00E-05	Nil	Australia	MA31	GAAGGCCTTCCGATGATC	144.90582
BC15841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84667	WGS84	BC15841	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.881	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15841_Upin_Bot2011_COI	MA31	0	BC15841_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15841	Sample	BC15841	targeted	Present	Sample	Upin_Bot2011_COI	MA31	COI	45	BC15841	1.00E-05	Nil	Australia	MA31	CTTTAATTACAGCGTTTTTATTGTTGT	144.90582
BC15842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81938	WGS84	BC15842	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	8/01/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15842_Mgig_Bot2012_COI	MA3	0	BC15842_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15842	Sample	BC15842	targeted	Absent	Sample	Mgig_Bot2012_COI	MA3	COI	45	BC15842	1.00E-05	Nil	Australia	MA3	TCTTATTCGTTGGAGACTTTATAACCCT	144.92775
BC15843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84537	WGS84	BC15843	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15843_Cmae_Bot2011_COI	MA29	0	BC15843_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15843	Sample	BC15843	targeted	Absent	Sample	Cmae_Bot2011_COI	MA29	COI	45	BC15843	1.00E-05	Nil	Australia	MA29	ATGAACAGTCTATCCTCCTTTAG	144.9053
BC15845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84523	WGS84	BC15845	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	26.883	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15845_Aamu_Bax2006_COI	MA23	0	BC15845_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15845	Sample	BC15845	targeted	Present	Sample	Aamu_Bax2006_COI	MA23	COI	45	BC15845	1.00E-05	Nil	Australia	MA23	GCACAACCGGGATCTTTACTTC	144.90766
BC15845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84523	WGS84	BC15845	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.498	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15845_Sspa_Oph2007_COI	MA23	0	BC15845_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15845	Sample	BC15845	targeted	Present	Sample	Sspa_Oph2007_COI	MA23	COI	45	BC15845	1.00E-05	Nil	Australia	MA23	GAAGGCCTTCCGATGATC	144.90766
BC15845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84523	WGS84	BC15845	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.455	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15845_Upin_Bot2011_COI	MA23	0	BC15845_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15845	Sample	BC15845	targeted	Present	Sample	Upin_Bot2011_COI	MA23	COI	45	BC15845	1.00E-05	Nil	Australia	MA23	CTTTAATTACAGCGTTTTTATTGTTGT	144.90766
BC15846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84488	WGS84	BC15846	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15846_Mgig_Bot2012_COI	MA124	0	BC15846_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15846	Sample	BC15846	targeted	Absent	Sample	Mgig_Bot2012_COI	MA124	COI	45	BC15846	1.00E-05	Nil	Australia	MA124	TCTTATTCGTTGGAGACTTTATAACCCT	144.93149
BC15847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84461	WGS84	BC15847	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15847_Cmae_Bot2011_COI	MA123	0	BC15847_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15847	Sample	BC15847	targeted	Absent	Sample	Cmae_Bot2011_COI	MA123	COI	45	BC15847	1.00E-05	Nil	Australia	MA123	ATGAACAGTCTATCCTCCTTTAG	144.92611
BC15849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86282	WGS84	BC15849	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.663	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15849_Aamu_Bax2006_COI	MA12	0	BC15849_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15849	Sample	BC15849	targeted	Present	Sample	Aamu_Bax2006_COI	MA12	COI	45	BC15849	1.00E-05	Nil	Australia	MA12	GCACAACCGGGATCTTTACTTC	144.91377
BC15849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86282	WGS84	BC15849	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.031	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15849_Sspa_Oph2007_COI	MA12	0	BC15849_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15849	Sample	BC15849	targeted	Present	Sample	Sspa_Oph2007_COI	MA12	COI	45	BC15849	1.00E-05	Nil	Australia	MA12	GAAGGCCTTCCGATGATC	144.91377
BC15849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86282	WGS84	BC15849	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.029	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	31/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15849_Upin_Bot2011_COI	MA12	0	BC15849_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15849	Sample	BC15849	targeted	Present	Sample	Upin_Bot2011_COI	MA12	COI	45	BC15849	1.00E-05	Nil	Australia	MA12	CTTTAATTACAGCGTTTTTATTGTTGT	144.91377
BC15850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8436	WGS84	BC15850	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15850_Cmae_Bot2011_COI	MA114	0	BC15850_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15850	Sample	BC15850	targeted	Absent	Sample	Cmae_Bot2011_COI	MA114	COI	45	BC15850	1.00E-05	Nil	Australia	MA114	ATGAACAGTCTATCCTCCTTTAG	144.93102
BC15852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84545	WGS84	BC15852	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.017	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15852_Aamu_Bax2006_COI	MA111	0	BC15852_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15852	Sample	BC15852	targeted	Present	Sample	Aamu_Bax2006_COI	MA111	COI	45	BC15852	1.00E-05	Nil	Australia	MA111	GCACAACCGGGATCTTTACTTC	144.93045
BC15852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84545	WGS84	BC15852	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.051	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15852_Sspa_Oph2007_COI	MA111	0	BC15852_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15852	Sample	BC15852	targeted	Present	Sample	Sspa_Oph2007_COI	MA111	COI	45	BC15852	1.00E-05	Nil	Australia	MA111	GAAGGCCTTCCGATGATC	144.93045
BC15852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84545	WGS84	BC15852	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.917	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15852_Upin_Bot2011_COI	MA111	0	BC15852_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15852	Sample	BC15852	targeted	Present	Sample	Upin_Bot2011_COI	MA111	COI	45	BC15852	1.00E-05	Nil	Australia	MA111	CTTTAATTACAGCGTTTTTATTGTTGT	144.93045
BC15853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82671	WGS84	BC15853	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	38.338	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15853_Mgig_Bot2012_COI	MA109	0	BC15853_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15853	Sample	BC15853	targeted	Present	Sample	Mgig_Bot2012_COI	MA109	COI	45	BC15853	1.00E-05	Nil	Australia	MA109	TCTTATTCGTTGGAGACTTTATAACCCT	144.89983
BC15854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8183	WGS84	BC15854	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	29/07/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15854_Cmae_Bot2011_COI	MA103	0	BC15854_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15854	Sample	BC15854	targeted	Absent	Sample	Cmae_Bot2011_COI	MA103	COI	45	BC15854	1.00E-05	Nil	Australia	MA103	ATGAACAGTCTATCCTCCTTTAG	144.92655
BC15856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84654	WGS84	BC15856	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.989	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15856_Aamu_Bax2006_COI	MA101	0	BC15856_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15856	Sample	BC15856	targeted	Present	Sample	Aamu_Bax2006_COI	MA101	COI	45	BC15856	1.00E-05	Nil	Australia	MA101	GCACAACCGGGATCTTTACTTC	144.93095
BC15856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84654	WGS84	BC15856	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.02	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15856_Sspa_Oph2007_COI	MA101	0	BC15856_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15856	Sample	BC15856	targeted	Present	Sample	Sspa_Oph2007_COI	MA101	COI	45	BC15856	1.00E-05	Nil	Australia	MA101	GAAGGCCTTCCGATGATC	144.93095
BC15856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84654	WGS84	BC15856	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	37.183	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Winter	30/07/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15856_Upin_Bot2011_COI	MA101	0	BC15856_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15856	Sample	BC15856	targeted	Present	Sample	Upin_Bot2011_COI	MA101	COI	45	BC15856	1.00E-05	Nil	Australia	MA101	CTTTAATTACAGCGTTTTTATTGTTGT	144.93095
BC15857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88505	WGS84	BC15857	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	6/08/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15857_Mgig_Bot2012_COI	HD18	0	BC15857_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15857	Sample	BC15857	targeted	Absent	Sample	Mgig_Bot2012_COI	HD18	COI	45	BC15857	1.00E-05	Nil	Australia	HD18	TCTTATTCGTTGGAGACTTTATAACCCT	147.33662
BC15858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8768	WGS84	BC15858	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Autumn	15/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15858_Cmae_Bot2011_COI	HD12	0	BC15858_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15858	Sample	BC15858	targeted	Absent	Sample	Cmae_Bot2011_COI	HD12	COI	45	BC15858	1.00E-05	Nil	Australia	HD12	ATGAACAGTCTATCCTCCTTTAG	147.34188
BC15860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8446	WGS84	BC15860	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.733	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15860_Mgig_Bot2012_COI	HC7	0	BC15860_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15860	Sample	BC15860	targeted	Present	Sample	Mgig_Bot2012_COI	HC7	COI	45	BC15860	1.00E-05	Nil	Australia	HC7	TCTTATTCGTTGGAGACTTTATAACCCT	147.3313
BC15861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8434	WGS84	BC15861	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15861_Cmae_Bot2011_COI	HC6	0	BC15861_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15861	Sample	BC15861	targeted	Absent	Sample	Cmae_Bot2011_COI	HC6	COI	45	BC15861	1.00E-05	Nil	Australia	HC6	ATGAACAGTCTATCCTCCTTTAG	147.3301
BC15863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8759	WGS84	BC15863	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15863_Aamu_Bax2006_COI	HC21	0	BC15863_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15863	Sample	BC15863	targeted	Absent	Sample	Aamu_Bax2006_COI	HC21	COI	45	BC15863	1.00E-05	Nil	Australia	HC21	GCACAACCGGGATCTTTACTTC	147.3413
BC15863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8759	WGS84	BC15863	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15863_Sspa_Oph2007_COI	HC21	0	BC15863_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15863	Sample	BC15863	targeted	Absent	Sample	Sspa_Oph2007_COI	HC21	COI	45	BC15863	1.00E-05	Nil	Australia	HC21	GAAGGCCTTCCGATGATC	147.3413
BC15863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8759	WGS84	BC15863	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15863_Upin_Bot2011_COI	HC21	0	BC15863_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15863	Sample	BC15863	targeted	Absent	Sample	Upin_Bot2011_COI	HC21	COI	45	BC15863	1.00E-05	Nil	Australia	HC21	CTTTAATTACAGCGTTTTTATTGTTGT	147.3413
BC15864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8293	WGS84	BC15864	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.018	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15864_Mgig_Bot2012_COI	HC2	0	BC15864_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15864	Sample	BC15864	targeted	Present	Sample	Mgig_Bot2012_COI	HC2	COI	45	BC15864	1.00E-05	Nil	Australia	HC2	TCTTATTCGTTGGAGACTTTATAACCCT	147.3182
BC15865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.8802	WGS84	BC15865	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Summer	14/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15865_Cmae_Bot2011_COI	HC18	0	BC15865_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15865	Sample	BC15865	targeted	Absent	Sample	Cmae_Bot2011_COI	HC18	COI	45	BC15865	1.00E-05	Nil	Australia	HC18	ATGAACAGTCTATCCTCCTTTAG	147.3419
BC15867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88525	WGS84	BC15867	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.066	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15867_Aamu_Bax2006_COI	HB4	0	BC15867_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15867	Sample	BC15867	targeted	Present	Sample	Aamu_Bax2006_COI	HB4	COI	45	BC15867	1.00E-05	Nil	Australia	HB4	GCACAACCGGGATCTTTACTTC	147.33569
BC15867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88525	WGS84	BC15867	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15867_Sspa_Oph2007_COI	HB4	0	BC15867_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15867	Sample	BC15867	targeted	Absent	Sample	Sspa_Oph2007_COI	HB4	COI	45	BC15867	1.00E-05	Nil	Australia	HB4	GAAGGCCTTCCGATGATC	147.33569
BC15867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88525	WGS84	BC15867	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15867_Upin_Bot2011_COI	HB4	0	BC15867_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15867	Sample	BC15867	targeted	Absent	Sample	Upin_Bot2011_COI	HB4	COI	45	BC15867	1.00E-05	Nil	Australia	HB4	CTTTAATTACAGCGTTTTTATTGTTGT	147.33569
BC15868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88502	WGS84	BC15868	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15868_Mgig_Bot2012_COI	HB3	0	BC15868_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15868	Sample	BC15868	targeted	Absent	Sample	Mgig_Bot2012_COI	HB3	COI	45	BC15868	1.00E-05	Nil	Australia	HB3	TCTTATTCGTTGGAGACTTTATAACCCT	147.33439
BC15869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.82939	WGS84	BC15869	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.532	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15869_Cmae_Bot2011_COI	HB23	0	BC15869_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15869	Sample	BC15869	targeted	Present	Sample	Cmae_Bot2011_COI	HB23	COI	45	BC15869	1.00E-05	Nil	Australia	HB23	ATGAACAGTCTATCCTCCTTTAG	147.31869
BC15870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88582	WGS84	BC15870	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	31.028	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15870_Aamu_Bax2006_COI	HB2	0	BC15870_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15870	Sample	BC15870	targeted	Present	Sample	Aamu_Bax2006_COI	HB2	COI	45	BC15870	1.00E-05	Nil	Australia	HB2	GCACAACCGGGATCTTTACTTC	147.33562
BC15870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88582	WGS84	BC15870	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15870_Sspa_Oph2007_COI	HB2	0	BC15870_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15870	Sample	BC15870	targeted	Absent	Sample	Sspa_Oph2007_COI	HB2	COI	45	BC15870	1.00E-05	Nil	Australia	HB2	GAAGGCCTTCCGATGATC	147.33562
BC15870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88582	WGS84	BC15870	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15870_Upin_Bot2011_COI	HB2	0	BC15870_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15870	Sample	BC15870	targeted	Absent	Sample	Upin_Bot2011_COI	HB2	COI	45	BC15870	1.00E-05	Nil	Australia	HB2	CTTTAATTACAGCGTTTTTATTGTTGT	147.33562
BC15871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84429	WGS84	BC15871	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15871_Mgig_Bot2012_COI	HB19	0	BC15871_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15871	Sample	BC15871	targeted	Absent	Sample	Mgig_Bot2012_COI	HB19	COI	45	BC15871	1.00E-05	Nil	Australia	HB19	TCTTATTCGTTGGAGACTTTATAACCCT	147.33007
BC15872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.84554	WGS84	BC15872	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.424	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15872_Cmae_Bot2011_COI	HB17	0	BC15872_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15872	Sample	BC15872	targeted	Present	Sample	Cmae_Bot2011_COI	HB17	COI	45	BC15872	1.00E-05	Nil	Australia	HB17	ATGAACAGTCTATCCTCCTTTAG	147.32933
BC15874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88666	WGS84	BC15874	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.316	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15874_Aamu_Bax2006_COI	HB11	0	BC15874_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15874	Sample	BC15874	targeted	Present	Sample	Aamu_Bax2006_COI	HB11	COI	45	BC15874	1.00E-05	Nil	Australia	HB11	GCACAACCGGGATCTTTACTTC	147.33999
BC15874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88666	WGS84	BC15874	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15874_Sspa_Oph2007_COI	HB11	0	BC15874_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15874	Sample	BC15874	targeted	Absent	Sample	Sspa_Oph2007_COI	HB11	COI	45	BC15874	1.00E-05	Nil	Australia	HB11	GAAGGCCTTCCGATGATC	147.33999
BC15874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-42.88666	WGS84	BC15874	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Hobart_Spring	11/06/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15874_Upin_Bot2011_COI	HB11	0	BC15874_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15874	Sample	BC15874	targeted	Absent	Sample	Upin_Bot2011_COI	HB11	COI	45	BC15874	1.00E-05	Nil	Australia	HB11	CTTTAATTACAGCGTTTTTATTGTTGT	147.33999
BC15875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8191	WGS84	BC15875	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.829	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15875_Mgig_Bot2012_COI	MC69	0	BC15875_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15875	Sample	BC15875	targeted	Present	Sample	Mgig_Bot2012_COI	MC69	COI	45	BC15875	1.00E-05	Nil	Australia	MC69	TCTTATTCGTTGGAGACTTTATAACCCT	144.92265
BC15876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81962	WGS84	BC15876	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15876_Cmae_Bot2011_COI	MD99	0	BC15876_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15876	Sample	BC15876	targeted	Absent	Sample	Cmae_Bot2011_COI	MD99	COI	45	BC15876	1.00E-05	Nil	Australia	MD99	ATGAACAGTCTATCCTCCTTTAG	144.91304
BC15878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81277	WGS84	BC15878	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.174	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15878_Aamu_Bax2006_COI	MD92	0	BC15878_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15878	Sample	BC15878	targeted	Present	Sample	Aamu_Bax2006_COI	MD92	COI	45	BC15878	1.00E-05	Nil	Australia	MD92	GCACAACCGGGATCTTTACTTC	144.91297
BC15878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81277	WGS84	BC15878	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.593	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15878_Sspa_Oph2007_COI	MD92	0	BC15878_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15878	Sample	BC15878	targeted	Present	Sample	Sspa_Oph2007_COI	MD92	COI	45	BC15878	1.00E-05	Nil	Australia	MD92	GAAGGCCTTCCGATGATC	144.91297
BC15878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81277	WGS84	BC15878	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15878_Upin_Bot2011_COI	MD92	0	BC15878_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15878	Sample	BC15878	targeted	Absent	Sample	Upin_Bot2011_COI	MD92	COI	45	BC15878	1.00E-05	Nil	Australia	MD92	CTTTAATTACAGCGTTTTTATTGTTGT	144.91297
BC15879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81384	WGS84	BC15879	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15879_Mgig_Bot2012_COI	MD89	0	BC15879_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15879	Sample	BC15879	targeted	Absent	Sample	Mgig_Bot2012_COI	MD89	COI	45	BC15879	1.00E-05	Nil	Australia	MD89	TCTTATTCGTTGGAGACTTTATAACCCT	144.91298
BC15881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81912	WGS84	BC15881	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.138	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15881_Aamu_Bax2006_COI	MD84	0	BC15881_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15881	Sample	BC15881	targeted	Present	Sample	Aamu_Bax2006_COI	MD84	COI	45	BC15881	1.00E-05	Nil	Australia	MD84	GCACAACCGGGATCTTTACTTC	144.9153
BC15881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81912	WGS84	BC15881	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.485	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15881_Sspa_Oph2007_COI	MD84	0	BC15881_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15881	Sample	BC15881	targeted	Present	Sample	Sspa_Oph2007_COI	MD84	COI	45	BC15881	1.00E-05	Nil	Australia	MD84	GAAGGCCTTCCGATGATC	144.9153
BC15881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81912	WGS84	BC15881	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15881_Upin_Bot2011_COI	MD84	0	BC15881_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15881	Sample	BC15881	targeted	Absent	Sample	Upin_Bot2011_COI	MD84	COI	45	BC15881	1.00E-05	Nil	Australia	MD84	CTTTAATTACAGCGTTTTTATTGTTGT	144.9153
BC15882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15882	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15882_Mgig_Bot2012_COI	MD81	0	BC15882_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15882	Sample	BC15882	targeted	Absent	Sample	Mgig_Bot2012_COI	MD81	COI	45	BC15882	1.00E-05	Nil	Australia	MD81	TCTTATTCGTTGGAGACTTTATAACCCT	144.9188
BC15883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8428	WGS84	BC15883	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15883_Cmae_Bot2011_COI	MD8	0	BC15883_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15883	Sample	BC15883	targeted	Absent	Sample	Cmae_Bot2011_COI	MD8	COI	45	BC15883	1.00E-05	Nil	Australia	MD8	ATGAACAGTCTATCCTCCTTTAG	144.92954
BC15885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81826	WGS84	BC15885	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	25.764	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15885_Aamu_Bax2006_COI	MD76	0	BC15885_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15885	Sample	BC15885	targeted	Present	Sample	Aamu_Bax2006_COI	MD76	COI	45	BC15885	1.00E-05	Nil	Australia	MD76	GCACAACCGGGATCTTTACTTC	144.92264
BC15885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81826	WGS84	BC15885	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.458	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15885_Sspa_Oph2007_COI	MD76	0	BC15885_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15885	Sample	BC15885	targeted	Present	Sample	Sspa_Oph2007_COI	MD76	COI	45	BC15885	1.00E-05	Nil	Australia	MD76	GAAGGCCTTCCGATGATC	144.92264
BC15885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81826	WGS84	BC15885	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15885_Upin_Bot2011_COI	MD76	0	BC15885_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15885	Sample	BC15885	targeted	Absent	Sample	Upin_Bot2011_COI	MD76	COI	45	BC15885	1.00E-05	Nil	Australia	MD76	CTTTAATTACAGCGTTTTTATTGTTGT	144.92264
BC15886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81919	WGS84	BC15886	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15886_Mgig_Bot2012_COI	MD72	0	BC15886_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15886	Sample	BC15886	targeted	Absent	Sample	Mgig_Bot2012_COI	MD72	COI	45	BC15886	1.00E-05	Nil	Australia	MD72	TCTTATTCGTTGGAGACTTTATAACCCT	144.92412
BC15887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81794	WGS84	BC15887	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15887_Cmae_Bot2011_COI	MD69	0	BC15887_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15887	Sample	BC15887	targeted	Absent	Sample	Cmae_Bot2011_COI	MD69	COI	45	BC15887	1.00E-05	Nil	Australia	MD69	ATGAACAGTCTATCCTCCTTTAG	144.92451
BC15889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81561	WGS84	BC15889	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	27.313	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15889_Aamu_Bax2006_COI	MD66	0	BC15889_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15889	Sample	BC15889	targeted	Present	Sample	Aamu_Bax2006_COI	MD66	COI	45	BC15889	1.00E-05	Nil	Australia	MD66	GCACAACCGGGATCTTTACTTC	144.92688
BC15889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81561	WGS84	BC15889	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.221	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15889_Sspa_Oph2007_COI	MD66	0	BC15889_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15889	Sample	BC15889	targeted	Present	Sample	Sspa_Oph2007_COI	MD66	COI	45	BC15889	1.00E-05	Nil	Australia	MD66	GAAGGCCTTCCGATGATC	144.92688
BC15889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81561	WGS84	BC15889	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15889_Upin_Bot2011_COI	MD66	0	BC15889_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15889	Sample	BC15889	targeted	Absent	Sample	Upin_Bot2011_COI	MD66	COI	45	BC15889	1.00E-05	Nil	Australia	MD66	CTTTAATTACAGCGTTTTTATTGTTGT	144.92688
BC15890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81966	WGS84	BC15890	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15890_Cmae_Bot2011_COI	MD64	0	BC15890_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15890	Sample	BC15890	targeted	Absent	Sample	Cmae_Bot2011_COI	MD64	COI	45	BC15890	1.00E-05	Nil	Australia	MD64	ATGAACAGTCTATCCTCCTTTAG	144.92612
BC15892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81935	WGS84	BC15892	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.052	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15892_Aamu_Bax2006_COI	MD58	0	BC15892_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15892	Sample	BC15892	targeted	Present	Sample	Aamu_Bax2006_COI	MD58	COI	45	BC15892	1.00E-05	Nil	Australia	MD58	GCACAACCGGGATCTTTACTTC	144.92941
BC15892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81935	WGS84	BC15892	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.552	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15892_Sspa_Oph2007_COI	MD58	0	BC15892_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15892	Sample	BC15892	targeted	Present	Sample	Sspa_Oph2007_COI	MD58	COI	45	BC15892	1.00E-05	Nil	Australia	MD58	GAAGGCCTTCCGATGATC	144.92941
BC15892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81935	WGS84	BC15892	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15892_Upin_Bot2011_COI	MD58	0	BC15892_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15892	Sample	BC15892	targeted	Absent	Sample	Upin_Bot2011_COI	MD58	COI	45	BC15892	1.00E-05	Nil	Australia	MD58	CTTTAATTACAGCGTTTTTATTGTTGT	144.92941
BC15893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81884	WGS84	BC15893	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15893_Mgig_Bot2012_COI	MD56	0	BC15893_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15893	Sample	BC15893	targeted	Absent	Sample	Mgig_Bot2012_COI	MD56	COI	45	BC15893	1.00E-05	Nil	Australia	MD56	TCTTATTCGTTGGAGACTTTATAACCCT	144.93145
BC15894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86038	WGS84	BC15894	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15894_Cmae_Bot2011_COI	MD54	0	BC15894_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15894	Sample	BC15894	targeted	Absent	Sample	Cmae_Bot2011_COI	MD54	COI	45	BC15894	1.00E-05	Nil	Australia	MD54	ATGAACAGTCTATCCTCCTTTAG	144.90903
BC15896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86205	WGS84	BC15896	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	30.18	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15896_Aamu_Bax2006_COI	MD47	0	BC15896_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15896	Sample	BC15896	targeted	Present	Sample	Aamu_Bax2006_COI	MD47	COI	45	BC15896	1.00E-05	Nil	Australia	MD47	GCACAACCGGGATCTTTACTTC	144.91494
BC15896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86205	WGS84	BC15896	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.817	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15896_Sspa_Oph2007_COI	MD47	0	BC15896_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15896	Sample	BC15896	targeted	Present	Sample	Sspa_Oph2007_COI	MD47	COI	45	BC15896	1.00E-05	Nil	Australia	MD47	GAAGGCCTTCCGATGATC	144.91494
BC15896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86205	WGS84	BC15896	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.769	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15896_Upin_Bot2011_COI	MD47	0	BC15896_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15896	Sample	BC15896	targeted	Present	Sample	Upin_Bot2011_COI	MD47	COI	45	BC15896	1.00E-05	Nil	Australia	MD47	CTTTAATTACAGCGTTTTTATTGTTGT	144.91494
BC15897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86128	WGS84	BC15897	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15897_Mgig_Bot2012_COI	MD46	0	BC15897_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15897	Sample	BC15897	targeted	Absent	Sample	Mgig_Bot2012_COI	MD46	COI	45	BC15897	1.00E-05	Nil	Australia	MD46	TCTTATTCGTTGGAGACTTTATAACCCT	144.91286
BC15898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86314	WGS84	BC15898	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	26/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15898_Cmae_Bot2011_COI	MD42	0	BC15898_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15898	Sample	BC15898	targeted	Absent	Sample	Cmae_Bot2011_COI	MD42	COI	45	BC15898	1.00E-05	Nil	Australia	MD42	ATGAACAGTCTATCCTCCTTTAG	144.91721
BC15900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86111	WGS84	BC15900	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.186	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15900_Aamu_Bax2006_COI	MD34	0	BC15900_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15900	Sample	BC15900	targeted	Present	Sample	Aamu_Bax2006_COI	MD34	COI	45	BC15900	1.00E-05	Nil	Australia	MD34	GCACAACCGGGATCTTTACTTC	144.90524
BC15900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86111	WGS84	BC15900	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.657	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15900_Sspa_Oph2007_COI	MD34	0	BC15900_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15900	Sample	BC15900	targeted	Present	Sample	Sspa_Oph2007_COI	MD34	COI	45	BC15900	1.00E-05	Nil	Australia	MD34	GAAGGCCTTCCGATGATC	144.90524
BC15900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86111	WGS84	BC15900	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.26	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15900_Upin_Bot2011_COI	MD34	0	BC15900_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15900	Sample	BC15900	targeted	Present	Sample	Upin_Bot2011_COI	MD34	COI	45	BC15900	1.00E-05	Nil	Australia	MD34	CTTTAATTACAGCGTTTTTATTGTTGT	144.90524
BC15901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86453	WGS84	BC15901	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15901_Mgig_Bot2012_COI	MD32	0	BC15901_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15901	Sample	BC15901	targeted	Absent	Sample	Mgig_Bot2012_COI	MD32	COI	45	BC15901	1.00E-05	Nil	Australia	MD32	TCTTATTCGTTGGAGACTTTATAACCCT	144.91606
BC15902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86511	WGS84	BC15902	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15902_Cmae_Bot2011_COI	MD31	0	BC15902_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15902	Sample	BC15902	targeted	Absent	Sample	Cmae_Bot2011_COI	MD31	COI	45	BC15902	1.00E-05	Nil	Australia	MD31	ATGAACAGTCTATCCTCCTTTAG	144.91823
BC15904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85005	WGS84	BC15904	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	24.791	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15904_Aamu_Bax2006_COI	MD27	0	BC15904_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15904	Sample	BC15904	targeted	Present	Sample	Aamu_Bax2006_COI	MD27	COI	45	BC15904	1.00E-05	Nil	Australia	MD27	GCACAACCGGGATCTTTACTTC	144.91302
BC15904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85005	WGS84	BC15904	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.979	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15904_Sspa_Oph2007_COI	MD27	0	BC15904_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15904	Sample	BC15904	targeted	Present	Sample	Sspa_Oph2007_COI	MD27	COI	45	BC15904	1.00E-05	Nil	Australia	MD27	GAAGGCCTTCCGATGATC	144.91302
BC15904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85005	WGS84	BC15904	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.579	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15904_Upin_Bot2011_COI	MD27	0	BC15904_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15904	Sample	BC15904	targeted	Present	Sample	Upin_Bot2011_COI	MD27	COI	45	BC15904	1.00E-05	Nil	Australia	MD27	CTTTAATTACAGCGTTTTTATTGTTGT	144.91302
BC15905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84831	WGS84	BC15905	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15905_Mgig_Bot2012_COI	MD24	0	BC15905_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15905	Sample	BC15905	targeted	Absent	Sample	Mgig_Bot2012_COI	MD24	COI	45	BC15905	1.00E-05	Nil	Australia	MD24	TCTTATTCGTTGGAGACTTTATAACCCT	144.91069
BC15906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84846	WGS84	BC15906	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15906_Cmae_Bot2011_COI	MD22	0	BC15906_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15906	Sample	BC15906	targeted	Absent	Sample	Cmae_Bot2011_COI	MD22	COI	45	BC15906	1.00E-05	Nil	Australia	MD22	ATGAACAGTCTATCCTCCTTTAG	144.90827
BC15908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84019	WGS84	BC15908	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	23.5	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15908_Aamu_Bax2006_COI	MD13	0	BC15908_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15908	Sample	BC15908	targeted	Present	Sample	Aamu_Bax2006_COI	MD13	COI	45	BC15908	1.00E-05	Nil	Australia	MD13	GCACAACCGGGATCTTTACTTC	144.90569
BC15908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84019	WGS84	BC15908	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.471	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15908_Sspa_Oph2007_COI	MD13	0	BC15908_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15908	Sample	BC15908	targeted	Present	Sample	Sspa_Oph2007_COI	MD13	COI	45	BC15908	1.00E-05	Nil	Australia	MD13	GAAGGCCTTCCGATGATC	144.90569
BC15908	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84019	WGS84	BC15908	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	36.372	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15908_Upin_Bot2011_COI	MD13	0	BC15908_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15908	Sample	BC15908	targeted	Present	Sample	Upin_Bot2011_COI	MD13	COI	45	BC15908	1.00E-05	Nil	Australia	MD13	CTTTAATTACAGCGTTTTTATTGTTGT	144.90569
BC15909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84466	WGS84	BC15909	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15909_Mgig_Bot2012_COI	MD2	0	BC15909_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15909	Sample	BC15909	targeted	Absent	Sample	Mgig_Bot2012_COI	MD2	COI	45	BC15909	1.00E-05	Nil	Australia	MD2	TCTTATTCGTTGGAGACTTTATAACCCT	144.93199
BC15911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82593	WGS84	BC15911	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.158	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15911_Aamu_Bax2006_COI	MD122	0	BC15911_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15911	Sample	BC15911	targeted	Present	Sample	Aamu_Bax2006_COI	MD122	COI	45	BC15911	1.00E-05	Nil	Australia	MD122	GCACAACCGGGATCTTTACTTC	144.90066
BC15911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82593	WGS84	BC15911	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.411	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15911_Sspa_Oph2007_COI	MD122	0	BC15911_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15911	Sample	BC15911	targeted	Present	Sample	Sspa_Oph2007_COI	MD122	COI	45	BC15911	1.00E-05	Nil	Australia	MD122	GAAGGCCTTCCGATGATC	144.90066
BC15911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82593	WGS84	BC15911	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15911_Upin_Bot2011_COI	MD122	0	BC15911_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15911	Sample	BC15911	targeted	Absent	Sample	Upin_Bot2011_COI	MD122	COI	45	BC15911	1.00E-05	Nil	Australia	MD122	CTTTAATTACAGCGTTTTTATTGTTGT	144.90066
BC15912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82515	WGS84	BC15912	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15912_Mgig_Bot2012_COI	MD121	0	BC15912_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15912	Sample	BC15912	targeted	Absent	Sample	Mgig_Bot2012_COI	MD121	COI	45	BC15912	1.00E-05	Nil	Australia	MD121	TCTTATTCGTTGGAGACTTTATAACCCT	144.90263
BC15913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82415	WGS84	BC15913	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	28/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15913_Cmae_Bot2011_COI	MD118	0	BC15913_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15913	Sample	BC15913	targeted	Absent	Sample	Cmae_Bot2011_COI	MD118	COI	45	BC15913	1.00E-05	Nil	Australia	MD118	ATGAACAGTCTATCCTCCTTTAG	144.90236
BC15915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82204	WGS84	BC15915	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	28.532	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15915_Aamu_Bax2006_COI	MD114	0	BC15915_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15915	Sample	BC15915	targeted	Present	Sample	Aamu_Bax2006_COI	MD114	COI	45	BC15915	1.00E-05	Nil	Australia	MD114	GCACAACCGGGATCTTTACTTC	144.90544
BC15915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82204	WGS84	BC15915	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.91	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15915_Sspa_Oph2007_COI	MD114	0	BC15915_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15915	Sample	BC15915	targeted	Present	Sample	Sspa_Oph2007_COI	MD114	COI	45	BC15915	1.00E-05	Nil	Australia	MD114	GAAGGCCTTCCGATGATC	144.90544
BC15915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82204	WGS84	BC15915	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15915_Upin_Bot2011_COI	MD114	0	BC15915_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15915	Sample	BC15915	targeted	Absent	Sample	Upin_Bot2011_COI	MD114	COI	45	BC15915	1.00E-05	Nil	Australia	MD114	CTTTAATTACAGCGTTTTTATTGTTGT	144.90544
BC15916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81945	WGS84	BC15916	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15916_Mgig_Bot2012_COI	MD112	0	BC15916_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15916	Sample	BC15916	targeted	Absent	Sample	Mgig_Bot2012_COI	MD112	COI	45	BC15916	1.00E-05	Nil	Australia	MD112	TCTTATTCGTTGGAGACTTTATAACCCT	144.90621
BC15917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82111	WGS84	BC15917	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15917_Cmae_Bot2011_COI	MD109	0	BC15917_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15917	Sample	BC15917	targeted	Absent	Sample	Cmae_Bot2011_COI	MD109	COI	45	BC15917	1.00E-05	Nil	Australia	MD109	ATGAACAGTCTATCCTCCTTTAG	144.90803
BC15919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82053	WGS84	BC15919	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	29.292	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15919_Aamu_Bax2006_COI	MD102	0	BC15919_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15919	Sample	BC15919	targeted	Present	Sample	Aamu_Bax2006_COI	MD102	COI	45	BC15919	1.00E-05	Nil	Australia	MD102	GCACAACCGGGATCTTTACTTC	144.91301
BC15919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82053	WGS84	BC15919	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.77	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15919_Sspa_Oph2007_COI	MD102	0	BC15919_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15919	Sample	BC15919	targeted	Present	Sample	Sspa_Oph2007_COI	MD102	COI	45	BC15919	1.00E-05	Nil	Australia	MD102	GAAGGCCTTCCGATGATC	144.91301
BC15919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.82053	WGS84	BC15919	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	27/06/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15919_Upin_Bot2011_COI	MD102	0	BC15919_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15919	Sample	BC15919	targeted	Absent	Sample	Upin_Bot2011_COI	MD102	COI	45	BC15919	1.00E-05	Nil	Australia	MD102	CTTTAATTACAGCGTTTTTATTGTTGT	144.91301
BC15920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11740	-37.84404	WGS84	BC15920	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Autumn	25/06/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15920_Cmae_Bot2011_COI	MD1	0	BC15920_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15920	Sample	BC15920	targeted	Absent	Sample	Cmae_Bot2011_COI	MD1	COI	45	BC15920	1.00E-05	Nil	Australia	MD1	ATGAACAGTCTATCCTCCTTTAG	144.93298
BC15922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81548	WGS84	BC15922	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15922_Aamu_Bax2006_COI	MC93	0	BC15922_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15922	Sample	BC15922	targeted	Absent	Sample	Aamu_Bax2006_COI	MC93	COI	45	BC15922	1.00E-05	Nil	Australia	MC93	GCACAACCGGGATCTTTACTTC	144.91301
BC15922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81548	WGS84	BC15922	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.852	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15922_Sspa_Oph2007_COI	MC93	0	BC15922_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15922	Sample	BC15922	targeted	Present	Sample	Sspa_Oph2007_COI	MC93	COI	45	BC15922	1.00E-05	Nil	Australia	MC93	GAAGGCCTTCCGATGATC	144.91301
BC15922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81548	WGS84	BC15922	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15922_Upin_Bot2011_COI	MC93	0	BC15922_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15922	Sample	BC15922	targeted	Absent	Sample	Upin_Bot2011_COI	MC93	COI	45	BC15922	1.00E-05	Nil	Australia	MC93	CTTTAATTACAGCGTTTTTATTGTTGT	144.91301
BC15923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81339	WGS84	BC15923	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.367	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15923_Mgig_Bot2012_COI	MC92	0	BC15923_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15923	Sample	BC15923	targeted	Present	Sample	Mgig_Bot2012_COI	MC92	COI	45	BC15923	1.00E-05	Nil	Australia	MC92	TCTTATTCGTTGGAGACTTTATAACCCT	144.91286
BC15924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81823	WGS84	BC15924	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15924_Cmae_Bot2011_COI	MC86	0	BC15924_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15924	Sample	BC15924	targeted	Absent	Sample	Cmae_Bot2011_COI	MC86	COI	45	BC15924	1.00E-05	Nil	Australia	MC86	ATGAACAGTCTATCCTCCTTTAG	144.91989
BC15926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81972	WGS84	BC15926	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15926_Aamu_Bax2006_COI	MC83	0	BC15926_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15926	Sample	BC15926	targeted	Absent	Sample	Aamu_Bax2006_COI	MC83	COI	45	BC15926	1.00E-05	Nil	Australia	MC83	GCACAACCGGGATCTTTACTTC	144.92466
BC15926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81972	WGS84	BC15926	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.778	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15926_Sspa_Oph2007_COI	MC83	0	BC15926_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15926	Sample	BC15926	targeted	Present	Sample	Sspa_Oph2007_COI	MC83	COI	45	BC15926	1.00E-05	Nil	Australia	MC83	GAAGGCCTTCCGATGATC	144.92466
BC15926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81972	WGS84	BC15926	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15926_Upin_Bot2011_COI	MC83	0	BC15926_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15926	Sample	BC15926	targeted	Absent	Sample	Upin_Bot2011_COI	MC83	COI	45	BC15926	1.00E-05	Nil	Australia	MC83	CTTTAATTACAGCGTTTTTATTGTTGT	144.92466
BC15927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84373	WGS84	BC15927	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.894	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15927_Mgig_Bot2012_COI	MC8	0	BC15927_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15927	Sample	BC15927	targeted	Present	Sample	Mgig_Bot2012_COI	MC8	COI	45	BC15927	1.00E-05	Nil	Australia	MC8	TCTTATTCGTTGGAGACTTTATAACCCT	144.92868
BC15928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81642	WGS84	BC15928	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15928_Cmae_Bot2011_COI	MC79	0	BC15928_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15928	Sample	BC15928	targeted	Absent	Sample	Cmae_Bot2011_COI	MC79	COI	45	BC15928	1.00E-05	Nil	Australia	MC79	ATGAACAGTCTATCCTCCTTTAG	144.92438
BC15930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81842	WGS84	BC15930	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.344	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15930_Mgig_Bot2012_COI	MC74	0	BC15930_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15930	Sample	BC15930	targeted	Present	Sample	Mgig_Bot2012_COI	MC74	COI	45	BC15930	1.00E-05	Nil	Australia	MC74	TCTTATTCGTTGGAGACTTTATAACCCT	144.92982
BC15931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81897	WGS84	BC15931	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15931_Cmae_Bot2011_COI	MC71	0	BC15931_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15931	Sample	BC15931	targeted	Absent	Sample	Cmae_Bot2011_COI	MC71	COI	45	BC15931	1.00E-05	Nil	Australia	MC71	ATGAACAGTCTATCCTCCTTTAG	144.92921
BC15933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81948	WGS84	BC15933	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15933_Aamu_Bax2006_COI	MC63	0	BC15933_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15933	Sample	BC15933	targeted	Absent	Sample	Aamu_Bax2006_COI	MC63	COI	45	BC15933	1.00E-05	Nil	Australia	MC63	GCACAACCGGGATCTTTACTTC	144.91605
BC15933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81948	WGS84	BC15933	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.751	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15933_Sspa_Oph2007_COI	MC63	0	BC15933_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15933	Sample	BC15933	targeted	Present	Sample	Sspa_Oph2007_COI	MC63	COI	45	BC15933	1.00E-05	Nil	Australia	MC63	GAAGGCCTTCCGATGATC	144.91605
BC15933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81948	WGS84	BC15933	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15933_Upin_Bot2011_COI	MC63	0	BC15933_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15933	Sample	BC15933	targeted	Absent	Sample	Upin_Bot2011_COI	MC63	COI	45	BC15933	1.00E-05	Nil	Australia	MC63	CTTTAATTACAGCGTTTTTATTGTTGT	144.91605
BC15934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82262	WGS84	BC15934	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.154	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15934_Mgig_Bot2012_COI	MC61	0	BC15934_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15934	Sample	BC15934	targeted	Present	Sample	Mgig_Bot2012_COI	MC61	COI	45	BC15934	1.00E-05	Nil	Australia	MC61	TCTTATTCGTTGGAGACTTTATAACCCT	144.90604
BC15935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84165	WGS84	BC15935	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15935_Cmae_Bot2011_COI	MC6	0	BC15935_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15935	Sample	BC15935	targeted	Absent	Sample	Cmae_Bot2011_COI	MC6	COI	45	BC15935	1.00E-05	Nil	Australia	MC6	ATGAACAGTCTATCCTCCTTTAG	144.92945
BC15937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82384	WGS84	BC15937	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15937_Aamu_Bax2006_COI	MC58	0	BC15937_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15937	Sample	BC15937	targeted	Absent	Sample	Aamu_Bax2006_COI	MC58	COI	45	BC15937	1.00E-05	Nil	Australia	MC58	GCACAACCGGGATCTTTACTTC	144.90353
BC15937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82384	WGS84	BC15937	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.14	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15937_Sspa_Oph2007_COI	MC58	0	BC15937_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15937	Sample	BC15937	targeted	Present	Sample	Sspa_Oph2007_COI	MC58	COI	45	BC15937	1.00E-05	Nil	Australia	MC58	GAAGGCCTTCCGATGATC	144.90353
BC15937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82384	WGS84	BC15937	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15937_Upin_Bot2011_COI	MC58	0	BC15937_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15937	Sample	BC15937	targeted	Absent	Sample	Upin_Bot2011_COI	MC58	COI	45	BC15937	1.00E-05	Nil	Australia	MC58	CTTTAATTACAGCGTTTTTATTGTTGT	144.90353
BC15938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82375	WGS84	BC15938	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.58	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15938_Mgig_Bot2012_COI	MC57	0	BC15938_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15938	Sample	BC15938	targeted	Present	Sample	Mgig_Bot2012_COI	MC57	COI	45	BC15938	1.00E-05	Nil	Australia	MC57	TCTTATTCGTTGGAGACTTTATAACCCT	144.90284
BC15939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82484	WGS84	BC15939	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15939_Cmae_Bot2011_COI	MC56	0	BC15939_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15939	Sample	BC15939	targeted	Absent	Sample	Cmae_Bot2011_COI	MC56	COI	45	BC15939	1.00E-05	Nil	Australia	MC56	ATGAACAGTCTATCCTCCTTTAG	144.90287
BC15940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82722	WGS84	BC15940	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15940_Aamu_Bax2006_COI	MC51	0	BC15940_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15940	Sample	BC15940	targeted	Absent	Sample	Aamu_Bax2006_COI	MC51	COI	45	BC15940	1.00E-05	Nil	Australia	MC51	GCACAACCGGGATCTTTACTTC	144.90064
BC15940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82722	WGS84	BC15940	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.528	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15940_Sspa_Oph2007_COI	MC51	0	BC15940_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15940	Sample	BC15940	targeted	Present	Sample	Sspa_Oph2007_COI	MC51	COI	45	BC15940	1.00E-05	Nil	Australia	MC51	GAAGGCCTTCCGATGATC	144.90064
BC15940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82722	WGS84	BC15940	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15940_Upin_Bot2011_COI	MC51	0	BC15940_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15940	Sample	BC15940	targeted	Absent	Sample	Upin_Bot2011_COI	MC51	COI	45	BC15940	1.00E-05	Nil	Australia	MC51	CTTTAATTACAGCGTTTTTATTGTTGT	144.90064
BC15941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82723	WGS84	BC15941	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.433	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15941_Mgig_Bot2012_COI	MC49	0	BC15941_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15941	Sample	BC15941	targeted	Present	Sample	Mgig_Bot2012_COI	MC49	COI	45	BC15941	1.00E-05	Nil	Australia	MC49	TCTTATTCGTTGGAGACTTTATAACCCT	144.9018
BC15942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.8663	WGS84	BC15942	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	25/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15942_Cmae_Bot2011_COI	MC31	0	BC15942_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15942	Sample	BC15942	targeted	Absent	Sample	Cmae_Bot2011_COI	MC31	COI	45	BC15942	1.00E-05	Nil	Australia	MC31	ATGAACAGTCTATCCTCCTTTAG	144.91593
BC15944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84007	WGS84	BC15944	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15944_Aamu_Bax2006_COI	MC28	0	BC15944_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15944	Sample	BC15944	targeted	Absent	Sample	Aamu_Bax2006_COI	MC28	COI	45	BC15944	1.00E-05	Nil	Australia	MC28	GCACAACCGGGATCTTTACTTC	144.90559
BC15944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84007	WGS84	BC15944	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.155	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15944_Sspa_Oph2007_COI	MC28	0	BC15944_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15944	Sample	BC15944	targeted	Present	Sample	Sspa_Oph2007_COI	MC28	COI	45	BC15944	1.00E-05	Nil	Australia	MC28	GAAGGCCTTCCGATGATC	144.90559
BC15944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84007	WGS84	BC15944	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15944_Upin_Bot2011_COI	MC28	0	BC15944_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15944	Sample	BC15944	targeted	Absent	Sample	Upin_Bot2011_COI	MC28	COI	45	BC15944	1.00E-05	Nil	Australia	MC28	CTTTAATTACAGCGTTTTTATTGTTGT	144.90559
BC15945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84115	WGS84	BC15945	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.6	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	24/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15945_Mgig_Bot2012_COI	MC27	0	BC15945_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15945	Sample	BC15945	targeted	Present	Sample	Mgig_Bot2012_COI	MC27	COI	45	BC15945	1.00E-05	Nil	Australia	MC27	TCTTATTCGTTGGAGACTTTATAACCCT	144.90495
BC15946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84441	WGS84	BC15946	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15946_Cmae_Bot2011_COI	MC23	0	BC15946_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15946	Sample	BC15946	targeted	Absent	Sample	Cmae_Bot2011_COI	MC23	COI	45	BC15946	1.00E-05	Nil	Australia	MC23	ATGAACAGTCTATCCTCCTTTAG	144.90733
BC15948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84791	WGS84	BC15948	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15948_Aamu_Bax2006_COI	MC19	0	BC15948_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15948	Sample	BC15948	targeted	Absent	Sample	Aamu_Bax2006_COI	MC19	COI	45	BC15948	1.00E-05	Nil	Australia	MC19	GCACAACCGGGATCTTTACTTC	144.90758
BC15948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84791	WGS84	BC15948	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.253	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15948_Sspa_Oph2007_COI	MC19	0	BC15948_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15948	Sample	BC15948	targeted	Present	Sample	Sspa_Oph2007_COI	MC19	COI	45	BC15948	1.00E-05	Nil	Australia	MC19	GAAGGCCTTCCGATGATC	144.90758
BC15948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84791	WGS84	BC15948	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15948_Upin_Bot2011_COI	MC19	0	BC15948_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15948	Sample	BC15948	targeted	Absent	Sample	Upin_Bot2011_COI	MC19	COI	45	BC15948	1.00E-05	Nil	Australia	MC19	CTTTAATTACAGCGTTTTTATTGTTGT	144.90758
BC15949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84786	WGS84	BC15949	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.155	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15949_Mgig_Bot2012_COI	MC18	0	BC15949_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15949	Sample	BC15949	targeted	Present	Sample	Mgig_Bot2012_COI	MC18	COI	45	BC15949	1.00E-05	Nil	Australia	MC18	TCTTATTCGTTGGAGACTTTATAACCCT	144.90858
BC15951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85836	WGS84	BC15951	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15951_Aamu_Bax2006_COI	MC122	0	BC15951_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15951	Sample	BC15951	targeted	Absent	Sample	Aamu_Bax2006_COI	MC122	COI	45	BC15951	1.00E-05	Nil	Australia	MC122	GCACAACCGGGATCTTTACTTC	144.90732
BC15951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85836	WGS84	BC15951	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.6	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15951_Sspa_Oph2007_COI	MC122	0	BC15951_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15951	Sample	BC15951	targeted	Present	Sample	Sspa_Oph2007_COI	MC122	COI	45	BC15951	1.00E-05	Nil	Australia	MC122	GAAGGCCTTCCGATGATC	144.90732
BC15951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85836	WGS84	BC15951	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.944	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15951_Upin_Bot2011_COI	MC122	0	BC15951_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15951	Sample	BC15951	targeted	Present	Sample	Upin_Bot2011_COI	MC122	COI	45	BC15951	1.00E-05	Nil	Australia	MC122	CTTTAATTACAGCGTTTTTATTGTTGT	144.90732
BC15952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82163	WGS84	BC15952	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.649	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15952_Mgig_Bot2012_COI	MC116	0	BC15952_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15952	Sample	BC15952	targeted	Present	Sample	Mgig_Bot2012_COI	MC116	COI	45	BC15952	1.00E-05	Nil	Australia	MC116	TCTTATTCGTTGGAGACTTTATAACCCT	144.90604
BC15953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.85827	WGS84	BC15953	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15953_Cmae_Bot2011_COI	MC121	0	BC15953_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15953	Sample	BC15953	targeted	Absent	Sample	Cmae_Bot2011_COI	MC121	COI	45	BC15953	1.00E-05	Nil	Australia	MC121	ATGAACAGTCTATCCTCCTTTAG	144.90522
BC15955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82293	WGS84	BC15955	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	39.503	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15955_Aamu_Bax2006_COI	MC112	0	BC15955_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15955	Sample	BC15955	targeted	Present	Sample	Aamu_Bax2006_COI	MC112	COI	45	BC15955	1.00E-05	Nil	Australia	MC112	GCACAACCGGGATCTTTACTTC	144.90727
BC15955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82293	WGS84	BC15955	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.503	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15955_Sspa_Oph2007_COI	MC112	0	BC15955_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15955	Sample	BC15955	targeted	Present	Sample	Sspa_Oph2007_COI	MC112	COI	45	BC15955	1.00E-05	Nil	Australia	MC112	GAAGGCCTTCCGATGATC	144.90727
BC15955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.82293	WGS84	BC15955	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15955_Upin_Bot2011_COI	MC112	0	BC15955_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15955	Sample	BC15955	targeted	Absent	Sample	Upin_Bot2011_COI	MC112	COI	45	BC15955	1.00E-05	Nil	Australia	MC112	CTTTAATTACAGCGTTTTTATTGTTGT	144.90727
BC15956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81995	WGS84	BC15956	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.38	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15956_Mgig_Bot2012_COI	MC111	0	BC15956_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15956	Sample	BC15956	targeted	Present	Sample	Mgig_Bot2012_COI	MC111	COI	45	BC15956	1.00E-05	Nil	Australia	MC111	TCTTATTCGTTGGAGACTTTATAACCCT	144.91018
BC15957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.84766	WGS84	BC15957	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.341	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	23/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15957_Cmae_Bot2011_COI	MC11	0	BC15957_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15957	Sample	BC15957	targeted	Present	Sample	Cmae_Bot2011_COI	MC11	COI	45	BC15957	1.00E-05	Nil	Australia	MC11	ATGAACAGTCTATCCTCCTTTAG	144.93078
BC15959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81921	WGS84	BC15959	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15959_Aamu_Bax2006_COI	MC103	0	BC15959_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15959	Sample	BC15959	targeted	Absent	Sample	Aamu_Bax2006_COI	MC103	COI	45	BC15959	1.00E-05	Nil	Australia	MC103	GCACAACCGGGATCTTTACTTC	144.92594
BC15959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81921	WGS84	BC15959	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.597	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15959_Sspa_Oph2007_COI	MC103	0	BC15959_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15959	Sample	BC15959	targeted	Present	Sample	Sspa_Oph2007_COI	MC103	COI	45	BC15959	1.00E-05	Nil	Australia	MC103	GAAGGCCTTCCGATGATC	144.92594
BC15959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.81921	WGS84	BC15959	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Summer	27/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15959_Upin_Bot2011_COI	MC103	0	BC15959_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15959	Sample	BC15959	targeted	Absent	Sample	Upin_Bot2011_COI	MC103	COI	45	BC15959	1.00E-05	Nil	Australia	MC103	CTTTAATTACAGCGTTTTTATTGTTGT	144.92594
BC15960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81039	WGS84	BC15960	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15960_Cmae_Bot2011_COI	MB99	0	BC15960_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15960	Sample	BC15960	targeted	Absent	Sample	Cmae_Bot2011_COI	MB99	COI	45	BC15960	1.00E-05	Nil	Australia	MB99	ATGAACAGTCTATCCTCCTTTAG	144.91343
BC15962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81351	WGS84	BC15962	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15962_Aamu_Bax2006_COI	MB93	0	BC15962_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15962	Sample	BC15962	targeted	Absent	Sample	Aamu_Bax2006_COI	MB93	COI	45	BC15962	1.00E-05	Nil	Australia	MB93	GCACAACCGGGATCTTTACTTC	144.91416
BC15962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81351	WGS84	BC15962	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.033	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15962_Sspa_Oph2007_COI	MB93	0	BC15962_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15962	Sample	BC15962	targeted	Present	Sample	Sspa_Oph2007_COI	MB93	COI	45	BC15962	1.00E-05	Nil	Australia	MB93	GAAGGCCTTCCGATGATC	144.91416
BC15962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81351	WGS84	BC15962	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	41.098	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15962_Upin_Bot2011_COI	MB93	0	BC15962_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15962	Sample	BC15962	targeted	Present	Sample	Upin_Bot2011_COI	MB93	COI	45	BC15962	1.00E-05	Nil	Australia	MB93	CTTTAATTACAGCGTTTTTATTGTTGT	144.91416
BC15963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81548	WGS84	BC15963	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.708	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15963_Mgig_Bot2012_COI	MB91	0	BC15963_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15963	Sample	BC15963	targeted	Present	Sample	Mgig_Bot2012_COI	MB91	COI	45	BC15963	1.00E-05	Nil	Australia	MB91	TCTTATTCGTTGGAGACTTTATAACCCT	144.9145
BC15964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81861	WGS84	BC15964	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15964_Cmae_Bot2011_COI	MB88	0	BC15964_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15964	Sample	BC15964	targeted	Absent	Sample	Cmae_Bot2011_COI	MB88	COI	45	BC15964	1.00E-05	Nil	Australia	MB88	ATGAACAGTCTATCCTCCTTTAG	144.9163
BC15966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81667	WGS84	BC15966	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15966_Aamu_Bax2006_COI	MB84	0	BC15966_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15966	Sample	BC15966	targeted	Absent	Sample	Aamu_Bax2006_COI	MB84	COI	45	BC15966	1.00E-05	Nil	Australia	MB84	GCACAACCGGGATCTTTACTTC	144.92252
BC15966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81667	WGS84	BC15966	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15966_Sspa_Oph2007_COI	MB84	0	BC15966_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15966	Sample	BC15966	targeted	Absent	Sample	Sspa_Oph2007_COI	MB84	COI	45	BC15966	1.00E-05	Nil	Australia	MB84	GAAGGCCTTCCGATGATC	144.92252
BC15966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81667	WGS84	BC15966	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15966_Upin_Bot2011_COI	MB84	0	BC15966_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15966	Sample	BC15966	targeted	Absent	Sample	Upin_Bot2011_COI	MB84	COI	45	BC15966	1.00E-05	Nil	Australia	MB84	CTTTAATTACAGCGTTTTTATTGTTGT	144.92252
BC15967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81564	WGS84	BC15967	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.833	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15967_Mgig_Bot2012_COI	MB82	0	BC15967_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15967	Sample	BC15967	targeted	Present	Sample	Mgig_Bot2012_COI	MB82	COI	45	BC15967	1.00E-05	Nil	Australia	MB82	TCTTATTCGTTGGAGACTTTATAACCCT	144.92523
BC15968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84347	WGS84	BC15968	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15968_Cmae_Bot2011_COI	MB8	0	BC15968_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15968	Sample	BC15968	targeted	Absent	Sample	Cmae_Bot2011_COI	MB8	COI	45	BC15968	1.00E-05	Nil	Australia	MB8	ATGAACAGTCTATCCTCCTTTAG	144.90609
BC15970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.81794	WGS84	BC15970	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	41.166	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15970_Mgig_Bot2012_COI	MB74	0	BC15970_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15970	Sample	BC15970	targeted	Present	Sample	Mgig_Bot2012_COI	MB74	COI	45	BC15970	1.00E-05	Nil	Australia	MB74	TCTTATTCGTTGGAGACTTTATAACCCT	144.93041
BC15971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8205	WGS84	BC15971	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15971_Cmae_Bot2011_COI	MB68	0	BC15971_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15971	Sample	BC15971	targeted	Absent	Sample	Cmae_Bot2011_COI	MB68	COI	45	BC15971	1.00E-05	Nil	Australia	MB68	ATGAACAGTCTATCCTCCTTTAG	144.92968
BC15973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84301	WGS84	BC15973	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	33.492	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15973_Aamu_Bax2006_COI	MB6	0	BC15973_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15973	Sample	BC15973	targeted	Present	Sample	Aamu_Bax2006_COI	MB6	COI	45	BC15973	1.00E-05	Nil	Australia	MB6	GCACAACCGGGATCTTTACTTC	144.90526
BC15973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84301	WGS84	BC15973	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.564	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15973_Sspa_Oph2007_COI	MB6	0	BC15973_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15973	Sample	BC15973	targeted	Present	Sample	Sspa_Oph2007_COI	MB6	COI	45	BC15973	1.00E-05	Nil	Australia	MB6	GAAGGCCTTCCGATGATC	144.90526
BC15973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84301	WGS84	BC15973	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.779	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15973_Upin_Bot2011_COI	MB6	0	BC15973_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15973	Sample	BC15973	targeted	Present	Sample	Upin_Bot2011_COI	MB6	COI	45	BC15973	1.00E-05	Nil	Australia	MB6	CTTTAATTACAGCGTTTTTATTGTTGT	144.90526
BC15974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82323	WGS84	BC15974	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15974_Mgig_Bot2012_COI	MB59	0	BC15974_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15974	Sample	BC15974	targeted	Absent	Sample	Mgig_Bot2012_COI	MB59	COI	45	BC15974	1.00E-05	Nil	Australia	MB59	TCTTATTCGTTGGAGACTTTATAACCCT	144.90667
BC15975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.82651	WGS84	BC15975	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/11/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15975_Cmae_Bot2011_COI	MB57	0	BC15975_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15975	Sample	BC15975	targeted	Absent	Sample	Cmae_Bot2011_COI	MB57	COI	45	BC15975	1.00E-05	Nil	Australia	MB57	ATGAACAGTCTATCCTCCTTTAG	144.90242
BC15977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85954	WGS84	BC15977	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15977_Aamu_Bax2006_COI	MB51	0	BC15977_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15977	Sample	BC15977	targeted	Absent	Sample	Aamu_Bax2006_COI	MB51	COI	45	BC15977	1.00E-05	Nil	Australia	MB51	GCACAACCGGGATCTTTACTTC	144.90766
BC15977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85954	WGS84	BC15977	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.856	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15977_Sspa_Oph2007_COI	MB51	0	BC15977_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15977	Sample	BC15977	targeted	Present	Sample	Sspa_Oph2007_COI	MB51	COI	45	BC15977	1.00E-05	Nil	Australia	MB51	GAAGGCCTTCCGATGATC	144.90766
BC15977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.85954	WGS84	BC15977	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.391	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15977_Upin_Bot2011_COI	MB51	0	BC15977_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15977	Sample	BC15977	targeted	Present	Sample	Upin_Bot2011_COI	MB51	COI	45	BC15977	1.00E-05	Nil	Australia	MB51	CTTTAATTACAGCGTTTTTATTGTTGT	144.90766
BC15978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86119	WGS84	BC15978	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15978_Mgig_Bot2012_COI	MB48	0	BC15978_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15978	Sample	BC15978	targeted	Absent	Sample	Mgig_Bot2012_COI	MB48	COI	45	BC15978	1.00E-05	Nil	Australia	MB48	TCTTATTCGTTGGAGACTTTATAACCCT	144.90855
BC15979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86151	WGS84	BC15979	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15979_Cmae_Bot2011_COI	MB44	0	BC15979_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15979	Sample	BC15979	targeted	Absent	Sample	Cmae_Bot2011_COI	MB44	COI	45	BC15979	1.00E-05	Nil	Australia	MB44	ATGAACAGTCTATCCTCCTTTAG	144.90993
BC15980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86182	WGS84	BC15980	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.385	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15980_Aamu_Bax2006_COI	MB42	0	BC15980_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15980	Sample	BC15980	targeted	Present	Sample	Aamu_Bax2006_COI	MB42	COI	45	BC15980	1.00E-05	Nil	Australia	MB42	GCACAACCGGGATCTTTACTTC	144.91412
BC15980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86182	WGS84	BC15980	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.933	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15980_Sspa_Oph2007_COI	MB42	0	BC15980_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15980	Sample	BC15980	targeted	Present	Sample	Sspa_Oph2007_COI	MB42	COI	45	BC15980	1.00E-05	Nil	Australia	MB42	GAAGGCCTTCCGATGATC	144.91412
BC15980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.86182	WGS84	BC15980	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	30.39	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15980_Upin_Bot2011_COI	MB42	0	BC15980_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15980	Sample	BC15980	targeted	Present	Sample	Upin_Bot2011_COI	MB42	COI	45	BC15980	1.00E-05	Nil	Australia	MB42	CTTTAATTACAGCGTTTTTATTGTTGT	144.91412
BC15981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.8626	WGS84	BC15981	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15981_Mgig_Bot2012_COI	MB41	0	BC15981_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15981	Sample	BC15981	targeted	Absent	Sample	Mgig_Bot2012_COI	MB41	COI	45	BC15981	1.00E-05	Nil	Australia	MB41	TCTTATTCGTTGGAGACTTTATAACCCT	144.91343
BC15982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11734	-37.84074	WGS84	BC15982	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/08/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15982_Cmae_Bot2011_COI	MB4	0	BC15982_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15982	Sample	BC15982	targeted	Absent	Sample	Cmae_Bot2011_COI	MB4	COI	45	BC15982	1.00E-05	Nil	Australia	MB4	ATGAACAGTCTATCCTCCTTTAG	144.90425
BC15984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86568	WGS84	BC15984	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	37.98	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15984_Aamu_Bax2006_COI	MB36	0	BC15984_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15984	Sample	BC15984	targeted	Present	Sample	Aamu_Bax2006_COI	MB36	COI	45	BC15984	1.00E-05	Nil	Australia	MB36	GCACAACCGGGATCTTTACTTC	144.91795
BC15984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86568	WGS84	BC15984	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.394	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15984_Sspa_Oph2007_COI	MB36	0	BC15984_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15984	Sample	BC15984	targeted	Present	Sample	Sspa_Oph2007_COI	MB36	COI	45	BC15984	1.00E-05	Nil	Australia	MB36	GAAGGCCTTCCGATGATC	144.91795
BC15984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86568	WGS84	BC15984	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.486	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15984_Upin_Bot2011_COI	MB36	0	BC15984_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15984	Sample	BC15984	targeted	Present	Sample	Upin_Bot2011_COI	MB36	COI	45	BC15984	1.00E-05	Nil	Australia	MB36	CTTTAATTACAGCGTTTTTATTGTTGT	144.91795
BC15985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86493	WGS84	BC15985	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC15985_Mgig_Bot2012_COI	MB34	0	BC15985_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15985	Sample	BC15985	targeted	Absent	Sample	Mgig_Bot2012_COI	MB34	COI	45	BC15985	1.00E-05	Nil	Australia	MB34	TCTTATTCGTTGGAGACTTTATAACCCT	144.91762
BC15986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86421	WGS84	BC15986	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC15986_Cmae_Bot2011_COI	MB31	0	BC15986_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15986	Sample	BC15986	targeted	Absent	Sample	Cmae_Bot2011_COI	MB31	COI	45	BC15986	1.00E-05	Nil	Australia	MB31	ATGAACAGTCTATCCTCCTTTAG	144.91498
BC15988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86288	WGS84	BC15988	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.958	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC15988_Aamu_Bax2006_COI	MB29	0	BC15988_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15988	Sample	BC15988	targeted	Present	Sample	Aamu_Bax2006_COI	MB29	COI	45	BC15988	1.00E-05	Nil	Australia	MB29	GCACAACCGGGATCTTTACTTC	144.91667
BC15988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86288	WGS84	BC15988	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC15988_Sspa_Oph2007_COI	MB29	0	BC15988_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15988	Sample	BC15988	targeted	Absent	Sample	Sspa_Oph2007_COI	MB29	COI	45	BC15988	1.00E-05	Nil	Australia	MB29	GAAGGCCTTCCGATGATC	144.91667
BC15988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_11738	-37.86288	WGS84	BC15988	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.997	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Prowse T, Buss JJ, Giblot-Ducray D, Lieu YN, Deveney MR (2019) Diagnostic performance of molecular tests used to detect priority marine pests in plankton samples. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000337-1. SARDI Research Report Series No. 1036., Adelaide.	Molecular_validation_surveys-Melbourne_Spring	11/09/2017	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC15988_Upin_Bot2011_COI	MB29	0	BC15988_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC15988	Sample	BC15988	targeted	Present	Sample	Upin_Bot2011_COI	MB29	COI	45	BC15988	1.00E-05	Nil	Australia	MB29	CTTTAATTACAGCGTTTTTATTGTTGT	144.91667
BC25830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.81013	WGS84	BC25830	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25830_Cjap_Sim2018_COI	T18-04	1	BC25830_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25830	Sample	BC25830	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-04	COI	45	BC25830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.50249
BC25830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.81013	WGS84	BC25830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.202	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	28/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25830_Mgig_Bot2012_COI	T18-04	1	BC25830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25830	Sample	BC25830	targeted	Present	Sample	Mgig_Bot2012_COI	T18-04	COI	45	BC25830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.50249
BC25831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80376	WGS84	BC25831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.322	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25831_Cmae_Bot2011_COI	T18-05	1	BC25831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25831	Sample	BC25831	targeted	Present	Sample	Cmae_Bot2011_COI	T18-05	COI	45	BC25831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-05	ATGAACAGTCTATCCTCCTTTAG	147.52542
BC25833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80019	WGS84	BC25833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25833_Aamu_Bax2006_COI	T18-07	1	BC25833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25833	Sample	BC25833	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-07	COI	45	BC25833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-07	GCACAACCGGGATCTTTACTTC	147.46497
BC25833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80019	WGS84	BC25833	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25833_Pcan_Bot2011_IGS	T18-07	1	BC25833_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25833	Sample	BC25833	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-07	IGS	45	BC25833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-07	CGTAATCCTCAGTACTGGCTA	147.46497
BC25833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80019	WGS84	BC25833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25833_Sspa_Oph2007_COI	T18-07	1	BC25833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25833	Sample	BC25833	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-07	COI	45	BC25833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-07	GAAGGCCTTCCGATGATC	147.46497
BC25833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80019	WGS84	BC25833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25833_Upin_Bot2011_COI	T18-07	1	BC25833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25833	Sample	BC25833	targeted	Absent	Sample	Upin_Bot2011_COI	T18-07	COI	45	BC25833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.46497
BC25835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96402	WGS84	BC25835	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	33.753	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25835_Cmae_Bot2011_COI	T18-12	1	BC25835_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25835	Sample	BC25835	targeted	Present	Sample	Cmae_Bot2011_COI	T18-12	COI	45	BC25835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-12	ATGAACAGTCTATCCTCCTTTAG	147.53822
BC25837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25837	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	36.668	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25837_Aamu_Bax2006_COI	T18-14	1	BC25837_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25837	Sample	BC25837	targeted	Present	Sample	Aamu_Bax2006_COI	T18-14	COI	45	BC25837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-14	GCACAACCGGGATCTTTACTTC	147.52367
BC25837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25837	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25837_Pcan_Bot2011_IGS	T18-14	1	BC25837_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25837	Sample	BC25837	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-14	IGS	45	BC25837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-14	CGTAATCCTCAGTACTGGCTA	147.52367
BC25837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25837	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25837_Sspa_Oph2007_COI	T18-14	1	BC25837_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25837	Sample	BC25837	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-14	COI	45	BC25837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-14	GAAGGCCTTCCGATGATC	147.52367
BC25837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25837	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25837_Upin_Bot2011_COI	T18-14	1	BC25837_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25837	Sample	BC25837	targeted	Absent	Sample	Upin_Bot2011_COI	T18-14	COI	45	BC25837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-14	CTTTAATTACAGCGTTTTTATTGTTGT	147.52367
BC25838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25838	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	14/12/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25838_Cjap_Sim2018_COI	T18-15	1	BC25838_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25838	Sample	BC25838	targeted	Absent	Sample	Cjap_Sim2018_COI	T18-15	COI	45	BC25838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52367
BC25838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95993	WGS84	BC25838	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.951	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T18	14/12/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25838_Mgig_Bot2012_COI	T18-15	1	BC25838_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25838	Sample	BC25838	targeted	Present	Sample	Mgig_Bot2012_COI	T18-15	COI	45	BC25838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-15	TCTTATTCGTTGGAGACTTTATAACCCT	147.52367
BC25840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25840	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25840_Aamu_Bax2006_COI	T18-17	1	BC25840_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25840	Sample	BC25840	targeted	Absent	Sample	Aamu_Bax2006_COI	T18-17	COI	45	BC25840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-17	GCACAACCGGGATCTTTACTTC	147.48547
BC25840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25840	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25840_Pcan_Bot2011_IGS	T18-17	1	BC25840_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25840	Sample	BC25840	targeted	Absent	Sample	Pcan_Bot2011_IGS	T18-17	IGS	45	BC25840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-17	CGTAATCCTCAGTACTGGCTA	147.48547
BC25840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25840	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25840_Sspa_Oph2007_COI	T18-17	1	BC25840_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25840	Sample	BC25840	targeted	Absent	Sample	Sspa_Oph2007_COI	T18-17	COI	45	BC25840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-17	GAAGGCCTTCCGATGATC	147.48547
BC25840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25840	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T18	28/12/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25840_Upin_Bot2011_COI	T18-17	1	BC25840_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25840	Sample	BC25840	targeted	Absent	Sample	Upin_Bot2011_COI	T18-17	COI	45	BC25840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T18-17	CTTTAATTACAGCGTTTTTATTGTTGT	147.48547
BC25841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84676	WGS84	BC25841	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25841_Cjap_Sim2018_COI	S13-01	1	BC25841_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25841	Sample	BC25841	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-01	COI	45	BC25841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49723
BC25841	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84676	WGS84	BC25841	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.798	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25841_Mgig_Bot2012_COI	S13-01	1	BC25841_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25841	Sample	BC25841	targeted	Present	Sample	Mgig_Bot2012_COI	S13-01	COI	45	BC25841	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.49723
BC25842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84602	WGS84	BC25842	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.636	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25842_Cmae_Bot2011_COI	S13-02	1	BC25842_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25842	Sample	BC25842	targeted	Present	Sample	Cmae_Bot2011_COI	S13-02	COI	45	BC25842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-02	ATGAACAGTCTATCCTCCTTTAG	138.4969
BC25844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25844	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25844_Aamu_Bax2006_COI	S13-04	1	BC25844_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25844	Sample	BC25844	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-04	COI	45	BC25844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-04	GCACAACCGGGATCTTTACTTC	138.50784
BC25844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25844	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25844_Pcan_Bot2011_IGS	S13-04	1	BC25844_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25844	Sample	BC25844	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-04	IGS	45	BC25844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-04	CGTAATCCTCAGTACTGGCTA	138.50784
BC25844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25844	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.095	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25844_Sspa_Oph2007_COI	S13-04	1	BC25844_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25844	Sample	BC25844	targeted	Present	Sample	Sspa_Oph2007_COI	S13-04	COI	45	BC25844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-04	GAAGGCCTTCCGATGATC	138.50784
BC25844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83697	WGS84	BC25844	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25844_Upin_Bot2011_COI	S13-04	1	BC25844_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25844	Sample	BC25844	targeted	Absent	Sample	Upin_Bot2011_COI	S13-04	COI	45	BC25844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.50784
BC25845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83671	WGS84	BC25845	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25845_Cjap_Sim2018_COI	S13-05	1	BC25845_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25845	Sample	BC25845	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-05	COI	45	BC25845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50903
BC25845	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83671	WGS84	BC25845	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.398	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25845_Mgig_Bot2012_COI	S13-05	1	BC25845_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25845	Sample	BC25845	targeted	Present	Sample	Mgig_Bot2012_COI	S13-05	COI	45	BC25845	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.50903
BC25846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83708	WGS84	BC25846	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25846_Cmae_Bot2011_COI	S13-06	1	BC25846_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25846	Sample	BC25846	targeted	Absent	Sample	Cmae_Bot2011_COI	S13-06	COI	45	BC25846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-06	ATGAACAGTCTATCCTCCTTTAG	138.51062
BC25848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77898	WGS84	BC25848	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25848_Aamu_Bax2006_COI	S13-08	1	BC25848_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25848	Sample	BC25848	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-08	COI	45	BC25848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-08	GCACAACCGGGATCTTTACTTC	138.51781
BC25848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77898	WGS84	BC25848	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25848_Pcan_Bot2011_IGS	S13-08	1	BC25848_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25848	Sample	BC25848	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-08	IGS	45	BC25848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-08	CGTAATCCTCAGTACTGGCTA	138.51781
BC25848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77898	WGS84	BC25848	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25848_Sspa_Oph2007_COI	S13-08	1	BC25848_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25848	Sample	BC25848	targeted	Absent	Sample	Sspa_Oph2007_COI	S13-08	COI	45	BC25848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-08	GAAGGCCTTCCGATGATC	138.51781
BC25848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77898	WGS84	BC25848	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25848_Upin_Bot2011_COI	S13-08	1	BC25848_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25848	Sample	BC25848	targeted	Absent	Sample	Upin_Bot2011_COI	S13-08	COI	45	BC25848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.51781
BC25849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.78028	WGS84	BC25849	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25849_Cjap_Sim2018_COI	S13-09	1	BC25849_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25849	Sample	BC25849	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-09	COI	45	BC25849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51745
BC25849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.78028	WGS84	BC25849	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.285	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25849_Mgig_Bot2012_COI	S13-09	1	BC25849_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25849	Sample	BC25849	targeted	Present	Sample	Mgig_Bot2012_COI	S13-09	COI	45	BC25849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.51745
BC25851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80998	WGS84	BC25851	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25851_Aamu_Bax2006_COI	S13-11	1	BC25851_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25851	Sample	BC25851	targeted	Absent	Sample	Aamu_Bax2006_COI	S13-11	COI	45	BC25851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-11	GCACAACCGGGATCTTTACTTC	138.51973
BC25851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80998	WGS84	BC25851	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25851_Pcan_Bot2011_IGS	S13-11	1	BC25851_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25851	Sample	BC25851	targeted	Absent	Sample	Pcan_Bot2011_IGS	S13-11	IGS	45	BC25851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-11	CGTAATCCTCAGTACTGGCTA	138.51973
BC25851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80998	WGS84	BC25851	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.003	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25851_Sspa_Oph2007_COI	S13-11	1	BC25851_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25851	Sample	BC25851	targeted	Present	Sample	Sspa_Oph2007_COI	S13-11	COI	45	BC25851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-11	GAAGGCCTTCCGATGATC	138.51973
BC25851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80998	WGS84	BC25851	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25851_Upin_Bot2011_COI	S13-11	1	BC25851_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25851	Sample	BC25851	targeted	Absent	Sample	Upin_Bot2011_COI	S13-11	COI	45	BC25851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.51973
BC25852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81041	WGS84	BC25852	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S13	22/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25852_Cjap_Sim2018_COI	S13-12	1	BC25852_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25852	Sample	BC25852	targeted	Absent	Sample	Cjap_Sim2018_COI	S13-12	COI	45	BC25852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52065
BC25852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81041	WGS84	BC25852	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.071	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S13	22/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25852_Mgig_Bot2012_COI	S13-12	1	BC25852_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25852	Sample	BC25852	targeted	Present	Sample	Mgig_Bot2012_COI	S13-12	COI	45	BC25852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S13-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.52065
BC25853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83975	WGS84	BC25853	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25853_Cmae_Bot2011_COI	S14-01	1	BC25853_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25853	Sample	BC25853	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-01	COI	45	BC25853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-01	ATGAACAGTCTATCCTCCTTTAG	138.50678
BC25855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.8365	WGS84	BC25855	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25855_Aamu_Bax2006_COI	S14-03	1	BC25855_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25855	Sample	BC25855	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-03	COI	45	BC25855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-03	GCACAACCGGGATCTTTACTTC	138.50906
BC25855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.8365	WGS84	BC25855	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25855_Pcan_Bot2011_IGS	S14-03	1	BC25855_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25855	Sample	BC25855	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-03	IGS	45	BC25855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-03	CGTAATCCTCAGTACTGGCTA	138.50906
BC25855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.8365	WGS84	BC25855	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25855_Sspa_Oph2007_COI	S14-03	1	BC25855_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25855	Sample	BC25855	targeted	Absent	Sample	Sspa_Oph2007_COI	S14-03	COI	45	BC25855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-03	GAAGGCCTTCCGATGATC	138.50906
BC25855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.8365	WGS84	BC25855	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25855_Upin_Bot2011_COI	S14-03	1	BC25855_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25855	Sample	BC25855	targeted	Absent	Sample	Upin_Bot2011_COI	S14-03	COI	45	BC25855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.50906
BC25856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83589	WGS84	BC25856	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25856_Cjap_Sim2018_COI	S14-04	1	BC25856_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25856	Sample	BC25856	targeted	Absent	Sample	Cjap_Sim2018_COI	S14-04	COI	45	BC25856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50814
BC25856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83589	WGS84	BC25856	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.305	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S14	2/08/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25856_Mgig_Bot2012_COI	S14-04	1	BC25856_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25856	Sample	BC25856	targeted	Present	Sample	Mgig_Bot2012_COI	S14-04	COI	45	BC25856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.50814
BC25857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83382	WGS84	BC25857	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25857_Cmae_Bot2011_COI	S14-05	1	BC25857_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25857	Sample	BC25857	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-05	COI	45	BC25857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-05	ATGAACAGTCTATCCTCCTTTAG	138.50779
BC25859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80934	WGS84	BC25859	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25859_Aamu_Bax2006_COI	S14-07	1	BC25859_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25859	Sample	BC25859	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-07	COI	45	BC25859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-07	GCACAACCGGGATCTTTACTTC	138.51813
BC25859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80934	WGS84	BC25859	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25859_Pcan_Bot2011_IGS	S14-07	1	BC25859_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25859	Sample	BC25859	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-07	IGS	45	BC25859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-07	CGTAATCCTCAGTACTGGCTA	138.51813
BC25859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80934	WGS84	BC25859	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.905	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25859_Sspa_Oph2007_COI	S14-07	1	BC25859_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25859	Sample	BC25859	targeted	Present	Sample	Sspa_Oph2007_COI	S14-07	COI	45	BC25859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-07	GAAGGCCTTCCGATGATC	138.51813
BC25859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80934	WGS84	BC25859	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25859_Upin_Bot2011_COI	S14-07	1	BC25859_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25859	Sample	BC25859	targeted	Absent	Sample	Upin_Bot2011_COI	S14-07	COI	45	BC25859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.51813
BC25860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80988	WGS84	BC25860	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25860_Cmae_Bot2011_COI	S14-08	1	BC25860_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25860	Sample	BC25860	targeted	Absent	Sample	Cmae_Bot2011_COI	S14-08	COI	45	BC25860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-08	ATGAACAGTCTATCCTCCTTTAG	138.51941
BC25862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81126	WGS84	BC25862	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25862_Aamu_Bax2006_COI	S14-10	1	BC25862_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25862	Sample	BC25862	targeted	Absent	Sample	Aamu_Bax2006_COI	S14-10	COI	45	BC25862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-10	GCACAACCGGGATCTTTACTTC	138.52292
BC25862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81126	WGS84	BC25862	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25862_Pcan_Bot2011_IGS	S14-10	1	BC25862_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25862	Sample	BC25862	targeted	Absent	Sample	Pcan_Bot2011_IGS	S14-10	IGS	45	BC25862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-10	CGTAATCCTCAGTACTGGCTA	138.52292
BC25862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81126	WGS84	BC25862	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25862_Sspa_Oph2007_COI	S14-10	1	BC25862_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25862	Sample	BC25862	targeted	Absent	Sample	Sspa_Oph2007_COI	S14-10	COI	45	BC25862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-10	GAAGGCCTTCCGATGATC	138.52292
BC25862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.81126	WGS84	BC25862	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S14	2/08/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25862_Upin_Bot2011_COI	S14-10	1	BC25862_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25862	Sample	BC25862	targeted	Absent	Sample	Upin_Bot2011_COI	S14-10	COI	45	BC25862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S14-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.52292
BC25863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77237	WGS84	BC25863	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25863_Cjap_Sim2018_COI	S15-01	1	BC25863_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25863	Sample	BC25863	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-01	COI	45	BC25863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48606
BC25863	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77237	WGS84	BC25863	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.659	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25863_Mgig_Bot2012_COI	S15-01	1	BC25863_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25863	Sample	BC25863	targeted	Present	Sample	Mgig_Bot2012_COI	S15-01	COI	45	BC25863	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.48606
BC25864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77271	WGS84	BC25864	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25864_Cmae_Bot2011_COI	S15-02	1	BC25864_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25864	Sample	BC25864	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-02	COI	45	BC25864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-02	ATGAACAGTCTATCCTCCTTTAG	138.48516
BC25866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77797	WGS84	BC25866	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25866_Aamu_Bax2006_COI	S15-04	1	BC25866_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25866	Sample	BC25866	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-04	COI	45	BC25866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-04	GCACAACCGGGATCTTTACTTC	138.5189
BC25866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77797	WGS84	BC25866	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25866_Pcan_Bot2011_IGS	S15-04	1	BC25866_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25866	Sample	BC25866	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-04	IGS	45	BC25866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-04	CGTAATCCTCAGTACTGGCTA	138.5189
BC25866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77797	WGS84	BC25866	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25866_Sspa_Oph2007_COI	S15-04	1	BC25866_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25866	Sample	BC25866	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-04	COI	45	BC25866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-04	GAAGGCCTTCCGATGATC	138.5189
BC25866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77797	WGS84	BC25866	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25866_Upin_Bot2011_COI	S15-04	1	BC25866_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25866	Sample	BC25866	targeted	Absent	Sample	Upin_Bot2011_COI	S15-04	COI	45	BC25866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.5189
BC25867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77886	WGS84	BC25867	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25867_Cjap_Sim2018_COI	S15-05	1	BC25867_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25867	Sample	BC25867	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-05	COI	45	BC25867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51809
BC25867	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77886	WGS84	BC25867	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.856	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25867_Mgig_Bot2012_COI	S15-05	1	BC25867_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25867	Sample	BC25867	targeted	Present	Sample	Mgig_Bot2012_COI	S15-05	COI	45	BC25867	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.51809
BC25868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.77988	WGS84	BC25868	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25868_Cmae_Bot2011_COI	S15-06	1	BC25868_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25868	Sample	BC25868	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-06	COI	45	BC25868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-06	ATGAACAGTCTATCCTCCTTTAG	138.51786
BC25870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80904	WGS84	BC25870	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25870_Cjap_Sim2018_COI	S15-08	1	BC25870_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25870	Sample	BC25870	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-08	COI	45	BC25870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51774
BC25870	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80904	WGS84	BC25870	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.898	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25870_Mgig_Bot2012_COI	S15-08	1	BC25870_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25870	Sample	BC25870	targeted	Present	Sample	Mgig_Bot2012_COI	S15-08	COI	45	BC25870	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51774
BC25871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.80946	WGS84	BC25871	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25871_Cmae_Bot2011_COI	S15-09	1	BC25871_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25871	Sample	BC25871	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-09	COI	45	BC25871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-09	ATGAACAGTCTATCCTCCTTTAG	138.51863
BC25873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83683	WGS84	BC25873	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25873_Aamu_Bax2006_COI	S15-11	1	BC25873_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25873	Sample	BC25873	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-11	COI	45	BC25873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-11	GCACAACCGGGATCTTTACTTC	138.5099
BC25873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83683	WGS84	BC25873	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25873_Pcan_Bot2011_IGS	S15-11	1	BC25873_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25873	Sample	BC25873	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-11	IGS	45	BC25873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-11	CGTAATCCTCAGTACTGGCTA	138.5099
BC25873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83683	WGS84	BC25873	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25873_Sspa_Oph2007_COI	S15-11	1	BC25873_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25873	Sample	BC25873	targeted	Absent	Sample	Sspa_Oph2007_COI	S15-11	COI	45	BC25873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-11	GAAGGCCTTCCGATGATC	138.5099
BC25873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83683	WGS84	BC25873	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25873_Upin_Bot2011_COI	S15-11	1	BC25873_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25873	Sample	BC25873	targeted	Absent	Sample	Upin_Bot2011_COI	S15-11	COI	45	BC25873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-11	CTTTAATTACAGCGTTTTTATTGTTGT	138.5099
BC25874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83695	WGS84	BC25874	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25874_Cjap_Sim2018_COI	S15-12	1	BC25874_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25874	Sample	BC25874	targeted	Absent	Sample	Cjap_Sim2018_COI	S15-12	COI	45	BC25874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50914
BC25874	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.83695	WGS84	BC25874	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	26.374	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S15	25/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25874_Mgig_Bot2012_COI	S15-12	1	BC25874_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25874	Sample	BC25874	targeted	Present	Sample	Mgig_Bot2012_COI	S15-12	COI	45	BC25874	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-12	TCTTATTCGTTGGAGACTTTATAACCCT	138.50914
BC25875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84716	WGS84	BC25875	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25875_Cmae_Bot2011_COI	S15-13	1	BC25875_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25875	Sample	BC25875	targeted	Absent	Sample	Cmae_Bot2011_COI	S15-13	COI	45	BC25875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-13	ATGAACAGTCTATCCTCCTTTAG	138.49769
BC25877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84391	WGS84	BC25877	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25877_Aamu_Bax2006_COI	S15-15	1	BC25877_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25877	Sample	BC25877	targeted	Absent	Sample	Aamu_Bax2006_COI	S15-15	COI	45	BC25877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-15	GCACAACCGGGATCTTTACTTC	138.49688
BC25877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84391	WGS84	BC25877	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25877_Pcan_Bot2011_IGS	S15-15	1	BC25877_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25877	Sample	BC25877	targeted	Absent	Sample	Pcan_Bot2011_IGS	S15-15	IGS	45	BC25877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-15	CGTAATCCTCAGTACTGGCTA	138.49688
BC25877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84391	WGS84	BC25877	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.198	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25877_Sspa_Oph2007_COI	S15-15	1	BC25877_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25877	Sample	BC25877	targeted	Present	Sample	Sspa_Oph2007_COI	S15-15	COI	45	BC25877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-15	GAAGGCCTTCCGATGATC	138.49688
BC25877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-34.84391	WGS84	BC25877	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S15	25/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25877_Upin_Bot2011_COI	S15-15	1	BC25877_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25877	Sample	BC25877	targeted	Absent	Sample	Upin_Bot2011_COI	S15-15	COI	45	BC25877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S15-15	CTTTAATTACAGCGTTTTTATTGTTGT	138.49688
BC25878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96022	WGS84	BC25878	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25878_Cjap_Sim2018_COI	T19-01	1	BC25878_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25878	Sample	BC25878	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-01	COI	45	BC25878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52332
BC25878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96022	WGS84	BC25878	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.581	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	13/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25878_Mgig_Bot2012_COI	T19-01	1	BC25878_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25878	Sample	BC25878	targeted	Present	Sample	Mgig_Bot2012_COI	T19-01	COI	45	BC25878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-01	TCTTATTCGTTGGAGACTTTATAACCCT	147.52332
BC25879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96277	WGS84	BC25879	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25879_Cmae_Bot2011_COI	T19-02	1	BC25879_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25879	Sample	BC25879	targeted	Absent	Sample	Cmae_Bot2011_COI	T19-02	COI	45	BC25879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-02	ATGAACAGTCTATCCTCCTTTAG	147.53956
BC25880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.8101	WGS84	BC25880	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25880_Aamu_Bax2006_COI	T19-03	1	BC25880_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25880	Sample	BC25880	targeted	Absent	Sample	Aamu_Bax2006_COI	T19-03	COI	45	BC25880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-03	GCACAACCGGGATCTTTACTTC	147.50248
BC25880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.8101	WGS84	BC25880	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25880_Pcan_Bot2011_IGS	T19-03	1	BC25880_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25880	Sample	BC25880	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-03	IGS	45	BC25880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-03	CGTAATCCTCAGTACTGGCTA	147.50248
BC25880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.8101	WGS84	BC25880	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25880_Sspa_Oph2007_COI	T19-03	1	BC25880_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25880	Sample	BC25880	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-03	COI	45	BC25880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-03	GAAGGCCTTCCGATGATC	147.50248
BC25880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.8101	WGS84	BC25880	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25880_Upin_Bot2011_COI	T19-03	1	BC25880_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25880	Sample	BC25880	targeted	Absent	Sample	Upin_Bot2011_COI	T19-03	COI	45	BC25880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-03	CTTTAATTACAGCGTTTTTATTGTTGT	147.50248
BC25881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96467	WGS84	BC25881	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25881_Cjap_Sim2018_COI	T19-04	1	BC25881_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25881	Sample	BC25881	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-04	COI	45	BC25881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.51968
BC25881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96467	WGS84	BC25881	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.662	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	13/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25881_Mgig_Bot2012_COI	T19-04	1	BC25881_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25881	Sample	BC25881	targeted	Present	Sample	Mgig_Bot2012_COI	T19-04	COI	45	BC25881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-04	TCTTATTCGTTGGAGACTTTATAACCCT	147.51968
BC25882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95965	WGS84	BC25882	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.316	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	13/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25882_Cmae_Bot2011_COI	T19-05	1	BC25882_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25882	Sample	BC25882	targeted	Present	Sample	Cmae_Bot2011_COI	T19-05	COI	45	BC25882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-05	ATGAACAGTCTATCCTCCTTTAG	147.52786
BC25884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96014	WGS84	BC25884	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	34.631	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25884_Aamu_Bax2006_COI	T19-07	1	BC25884_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25884	Sample	BC25884	targeted	Present	Sample	Aamu_Bax2006_COI	T19-07	COI	45	BC25884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-07	GCACAACCGGGATCTTTACTTC	147.53014
BC25884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96014	WGS84	BC25884	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25884_Pcan_Bot2011_IGS	T19-07	1	BC25884_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25884	Sample	BC25884	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-07	IGS	45	BC25884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-07	CGTAATCCTCAGTACTGGCTA	147.53014
BC25884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96014	WGS84	BC25884	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25884_Sspa_Oph2007_COI	T19-07	1	BC25884_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25884	Sample	BC25884	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-07	COI	45	BC25884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-07	GAAGGCCTTCCGATGATC	147.53014
BC25884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96014	WGS84	BC25884	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25884_Upin_Bot2011_COI	T19-07	1	BC25884_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25884	Sample	BC25884	targeted	Absent	Sample	Upin_Bot2011_COI	T19-07	COI	45	BC25884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-07	CTTTAATTACAGCGTTTTTATTGTTGT	147.53014
BC25885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95987	WGS84	BC25885	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC25885_Cjap_Sim2018_COI	T19-08	1	BC25885_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25885	Sample	BC25885	targeted	Absent	Sample	Cjap_Sim2018_COI	T19-08	COI	45	BC25885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.52361
BC25885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.95987	WGS84	BC25885	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.956	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-T19	18/01/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC25885_Mgig_Bot2012_COI	T19-08	1	BC25885_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25885	Sample	BC25885	targeted	Present	Sample	Mgig_Bot2012_COI	T19-08	COI	45	BC25885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-08	TCTTATTCGTTGGAGACTTTATAACCCT	147.52361
BC25886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.96363	WGS84	BC25886	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.685	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	18/01/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25886_Cmae_Bot2011_COI	T19-09	1	BC25886_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25886	Sample	BC25886	targeted	Present	Sample	Cmae_Bot2011_COI	T19-09	COI	45	BC25886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-09	ATGAACAGTCTATCCTCCTTTAG	147.51932
BC25893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80378	WGS84	BC25893	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC25893_Cmae_Bot2011_COI	T19-16	1	BC25893_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25893	Sample	BC25893	targeted	Absent	Sample	Cmae_Bot2011_COI	T19-16	COI	45	BC25893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-16	ATGAACAGTCTATCCTCCTTTAG	147.5254
BC25895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC25895_Aamu_Bax2006_COI	T19-18	1	BC25895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25895	Sample	BC25895	targeted	Absent	Sample	Aamu_Bax2006_COI	T19-18	COI	45	BC25895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-18	GCACAACCGGGATCTTTACTTC	147.48557
BC25895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25895	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC25895_Pcan_Bot2011_IGS	T19-18	1	BC25895_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25895	Sample	BC25895	targeted	Absent	Sample	Pcan_Bot2011_IGS	T19-18	IGS	45	BC25895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-18	CGTAATCCTCAGTACTGGCTA	147.48557
BC25895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC25895_Sspa_Oph2007_COI	T19-18	1	BC25895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25895	Sample	BC25895	targeted	Absent	Sample	Sspa_Oph2007_COI	T19-18	COI	45	BC25895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-18	GAAGGCCTTCCGATGATC	147.48557
BC25895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12035	-42.80297	WGS84	BC25895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-T19	17/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC25895_Upin_Bot2011_COI	T19-18	1	BC25895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC25895	Sample	BC25895	targeted	Absent	Sample	Upin_Bot2011_COI	T19-18	COI	45	BC25895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	T19-18	CTTTAATTACAGCGTTTTTATTGTTGT	147.48557
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29598_Aamu_Bax2006_COI	KEMB2	1	BC29598_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	GCACAACCGGGATCTTTACTTC	150.91231
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29598_Pcan_Bot2011_IGS	KEMB2	1	BC29598_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB2	IGS	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	CGTAATCCTCAGTACTGGCTA	150.91231
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29598_Sspa_Oph2007_COI	KEMB2	1	BC29598_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	GAAGGCCTTCCGATGATC	150.91231
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29598_Upin_Bot2011_COI	KEMB2	1	BC29598_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	CTTTAATTACAGCGTTTTTATTGTTGT	150.91231
BC29598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47208	WGS84	BC29598	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29598_Vgib_Wil2025_COI	KEMB2	1	BC29598_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29598	Sample	BC29598	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB2	COI	45	BC29598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB2	CCCCTCTTTACAAGTTCTTTAGGGT	150.91231
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29599_Asen_Wil2025_COI	KEMB3	1	BC29599_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	GTTGTTGTTACAACACATGCTCTTTTG	150.9113
BC29599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47054	WGS84	BC29599	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29599_Mgig_Bot2012_COI	KEMB3	1	BC29599_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29599	Sample	BC29599	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB3	COI	45	BC29599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB3	TCTTATTCGTTGGAGACTTTATAACCCT	150.9113
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29600_Cmae_Bot2011_COI	KEMB4	1	BC29600_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	ATGAACAGTCTATCCTCCTTTAG	150.90917
BC29600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46992	WGS84	BC29600	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29600_Msal_Bot2012_COI	KEMB4	1	BC29600_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29600	Sample	BC29600	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB4	COI	45	BC29600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB4	GYTAGTTCCRATGATGTTAGCTG	150.90917
BC29602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88101	WGS84	BC29602	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29602_Aamu_Bax2006_COI	NEWA46	1	BC29602_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29602	Sample	BC29602	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA46	COI	45	BC29602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA46	GCACAACCGGGATCTTTACTTC	151.75063
BC29602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88101	WGS84	BC29602	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29602_Pcan_Bot2011_IGS	NEWA46	1	BC29602_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29602	Sample	BC29602	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA46	IGS	45	BC29602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA46	CGTAATCCTCAGTACTGGCTA	151.75063
BC29602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88101	WGS84	BC29602	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29602_Sspa_Oph2007_COI	NEWA46	1	BC29602_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29602	Sample	BC29602	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA46	COI	45	BC29602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA46	GAAGGCCTTCCGATGATC	151.75063
BC29602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88101	WGS84	BC29602	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29602_Upin_Bot2011_COI	NEWA46	1	BC29602_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29602	Sample	BC29602	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA46	COI	45	BC29602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA46	CTTTAATTACAGCGTTTTTATTGTTGT	151.75063
BC29608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25322	WGS84	BC29608	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29608_Cmae_Bot2011_COI	HAYA80	1	BC29608_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29608	Sample	BC29608	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA80	COI	45	BC29608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA80	ATGAACAGTCTATCCTCCTTTAG	149.30928
BC29608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25322	WGS84	BC29608	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29608_Msal_Bot2012_COI	HAYA80	1	BC29608_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29608	Sample	BC29608	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA80	COI	45	BC29608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA80	GYTAGTTCCRATGATGTTAGCTG	149.30928
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.2537	WGS84	BC29610	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29610_Asen_Wil2025_COI	HAYA78	1	BC29610_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	GTTGTTGTTACAACACATGCTCTTTTG	149.30538
BC29610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2537	WGS84	BC29610	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29610_Mgig_Bot2012_COI	HAYA78	1	BC29610_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29610	Sample	BC29610	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA78	COI	45	BC29610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA78	TCTTATTCGTTGGAGACTTTATAACCCT	149.30538
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.254	WGS84	BC29611	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29611_Cmae_Bot2011_COI	HAYA77	1	BC29611_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	ATGAACAGTCTATCCTCCTTTAG	149.31117
BC29611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.254	WGS84	BC29611	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29611_Msal_Bot2012_COI	HAYA77	1	BC29611_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29611	Sample	BC29611	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA77	COI	45	BC29611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA77	GYTAGTTCCRATGATGTTAGCTG	149.31117
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25406	WGS84	BC29613	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29613_Aamu_Bax2006_COI	HAYA75	1	BC29613_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	GCACAACCGGGATCTTTACTTC	149.30913
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25406	WGS84	BC29613	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29613_Pcan_Bot2011_IGS	HAYA75	1	BC29613_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA75	IGS	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	CGTAATCCTCAGTACTGGCTA	149.30913
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25406	WGS84	BC29613	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29613_Sspa_Oph2007_COI	HAYA75	1	BC29613_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	GAAGGCCTTCCGATGATC	149.30913
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25406	WGS84	BC29613	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29613_Upin_Bot2011_COI	HAYA75	1	BC29613_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	CTTTAATTACAGCGTTTTTATTGTTGT	149.30913
BC29613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25406	WGS84	BC29613	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29613_Vgib_Wil2025_COI	HAYA75	1	BC29613_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29613	Sample	BC29613	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA75	COI	45	BC29613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA75	CCCCTCTTTACAAGTTCTTTAGGGT	149.30913
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25515	WGS84	BC29614	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29614_Asen_Wil2025_COI	HAYA74	1	BC29614_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	GTTGTTGTTACAACACATGCTCTTTTG	149.30727
BC29614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25515	WGS84	BC29614	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29614_Mgig_Bot2012_COI	HAYA74	1	BC29614_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29614	Sample	BC29614	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA74	COI	45	BC29614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA74	TCTTATTCGTTGGAGACTTTATAACCCT	149.30727
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25489	WGS84	BC29615	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29615_Cmae_Bot2011_COI	HAYA73	1	BC29615_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	ATGAACAGTCTATCCTCCTTTAG	149.30571
BC29615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25489	WGS84	BC29615	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29615_Msal_Bot2012_COI	HAYA73	1	BC29615_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29615	Sample	BC29615	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA73	COI	45	BC29615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA73	GYTAGTTCCRATGATGTTAGCTG	149.30571
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25514	WGS84	BC29617	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29617_Aamu_Bax2006_COI	HAYA71	1	BC29617_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	GCACAACCGGGATCTTTACTTC	149.30984
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25514	WGS84	BC29617	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29617_Pcan_Bot2011_IGS	HAYA71	1	BC29617_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA71	IGS	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	CGTAATCCTCAGTACTGGCTA	149.30984
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25514	WGS84	BC29617	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29617_Sspa_Oph2007_COI	HAYA71	1	BC29617_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	GAAGGCCTTCCGATGATC	149.30984
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25514	WGS84	BC29617	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29617_Upin_Bot2011_COI	HAYA71	1	BC29617_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	CTTTAATTACAGCGTTTTTATTGTTGT	149.30984
BC29617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25514	WGS84	BC29617	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29617_Vgib_Wil2025_COI	HAYA71	1	BC29617_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29617	Sample	BC29617	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA71	COI	45	BC29617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA71	CCCCTCTTTACAAGTTCTTTAGGGT	149.30984
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25551	WGS84	BC29618	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29618_Asen_Wil2025_COI	HAYA70	1	BC29618_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	GTTGTTGTTACAACACATGCTCTTTTG	149.30925
BC29618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25551	WGS84	BC29618	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29618_Mgig_Bot2012_COI	HAYA70	1	BC29618_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29618	Sample	BC29618	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA70	COI	45	BC29618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA70	TCTTATTCGTTGGAGACTTTATAACCCT	149.30925
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25577	WGS84	BC29619	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29619_Cmae_Bot2011_COI	HAYA69	1	BC29619_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	ATGAACAGTCTATCCTCCTTTAG	149.30652
BC29619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25577	WGS84	BC29619	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29619_Msal_Bot2012_COI	HAYA69	1	BC29619_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29619	Sample	BC29619	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA69	COI	45	BC29619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA69	GYTAGTTCCRATGATGTTAGCTG	149.30652
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25554	WGS84	BC29620	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29620_Aamu_Bax2006_COI	HAYA68	1	BC29620_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	GCACAACCGGGATCTTTACTTC	149.31215
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25554	WGS84	BC29620	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29620_Pcan_Bot2011_IGS	HAYA68	1	BC29620_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA68	IGS	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	CGTAATCCTCAGTACTGGCTA	149.31215
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25554	WGS84	BC29620	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29620_Sspa_Oph2007_COI	HAYA68	1	BC29620_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	GAAGGCCTTCCGATGATC	149.31215
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25554	WGS84	BC29620	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29620_Upin_Bot2011_COI	HAYA68	1	BC29620_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	CTTTAATTACAGCGTTTTTATTGTTGT	149.31215
BC29620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25554	WGS84	BC29620	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29620_Vgib_Wil2025_COI	HAYA68	1	BC29620_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29620	Sample	BC29620	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA68	COI	45	BC29620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA68	CCCCTCTTTACAAGTTCTTTAGGGT	149.31215
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25542	WGS84	BC29621	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29621_Asen_Wil2025_COI	HAYA67	1	BC29621_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	GTTGTTGTTACAACACATGCTCTTTTG	149.31127
BC29621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25542	WGS84	BC29621	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29621_Mgig_Bot2012_COI	HAYA67	1	BC29621_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29621	Sample	BC29621	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA67	COI	45	BC29621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA67	TCTTATTCGTTGGAGACTTTATAACCCT	149.31127
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25536	WGS84	BC29622	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29622_Cmae_Bot2011_COI	HAYA66	1	BC29622_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	ATGAACAGTCTATCCTCCTTTAG	149.3102
BC29622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25536	WGS84	BC29622	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29622_Msal_Bot2012_COI	HAYA66	1	BC29622_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29622	Sample	BC29622	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA66	COI	45	BC29622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA66	GYTAGTTCCRATGATGTTAGCTG	149.3102
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25631	WGS84	BC29624	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29624_Aamu_Bax2006_COI	HAYA64	1	BC29624_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	GCACAACCGGGATCTTTACTTC	149.30702
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25631	WGS84	BC29624	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29624_Pcan_Bot2011_IGS	HAYA64	1	BC29624_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA64	IGS	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	CGTAATCCTCAGTACTGGCTA	149.30702
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25631	WGS84	BC29624	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29624_Sspa_Oph2007_COI	HAYA64	1	BC29624_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	GAAGGCCTTCCGATGATC	149.30702
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25631	WGS84	BC29624	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29624_Upin_Bot2011_COI	HAYA64	1	BC29624_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	CTTTAATTACAGCGTTTTTATTGTTGT	149.30702
BC29624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25631	WGS84	BC29624	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29624_Vgib_Wil2025_COI	HAYA64	1	BC29624_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29624	Sample	BC29624	targeted	Absent	Sample	Vgib_Wil2025_COI	HAYA64	COI	45	BC29624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA64	CCCCTCTTTACAAGTTCTTTAGGGT	149.30702
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16573	-21.25587	WGS84	BC29625	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29625_Asen_Wil2025_COI	HAYA63	1	BC29625_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Asen_Wil2025_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	GTTGTTGTTACAACACATGCTCTTTTG	149.31251
BC29625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25587	WGS84	BC29625	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29625_Mgig_Bot2012_COI	HAYA63	1	BC29625_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29625	Sample	BC29625	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA63	COI	45	BC29625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA63	TCTTATTCGTTGGAGACTTTATAACCCT	149.31251
BC29626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25676	WGS84	BC29626	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29626_Cmae_Bot2011_COI	HAYA62	1	BC29626_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29626	Sample	BC29626	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA62	COI	45	BC29626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA62	ATGAACAGTCTATCCTCCTTTAG	149.31127
BC29626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25676	WGS84	BC29626	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29626_Msal_Bot2012_COI	HAYA62	1	BC29626_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29626	Sample	BC29626	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA62	COI	45	BC29626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA62	GYTAGTTCCRATGATGTTAGCTG	149.31127
BC29628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25702	WGS84	BC29628	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29628_Aamu_Bax2006_COI	HAYA60	1	BC29628_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29628	Sample	BC29628	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA60	COI	45	BC29628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA60	GCACAACCGGGATCTTTACTTC	149.30984
BC29628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25702	WGS84	BC29628	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29628_Pcan_Bot2011_IGS	HAYA60	1	BC29628_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29628	Sample	BC29628	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA60	IGS	45	BC29628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA60	CGTAATCCTCAGTACTGGCTA	149.30984
BC29628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25702	WGS84	BC29628	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29628_Sspa_Oph2007_COI	HAYA60	1	BC29628_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29628	Sample	BC29628	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA60	COI	45	BC29628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA60	GAAGGCCTTCCGATGATC	149.30984
BC29628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25702	WGS84	BC29628	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29628_Upin_Bot2011_COI	HAYA60	1	BC29628_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29628	Sample	BC29628	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA60	COI	45	BC29628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA60	CTTTAATTACAGCGTTTTTATTGTTGT	149.30984
BC29629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25645	WGS84	BC29629	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	13/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29629_Mgig_Bot2012_COI	HAYA59	1	BC29629_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29629	Sample	BC29629	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA59	COI	45	BC29629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA59	TCTTATTCGTTGGAGACTTTATAACCCT	149.30918
BC29631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25743	WGS84	BC29631	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29631_Aamu_Bax2006_COI	HAYA57	1	BC29631_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29631	Sample	BC29631	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA57	COI	45	BC29631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA57	GCACAACCGGGATCTTTACTTC	149.3123
BC29631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25743	WGS84	BC29631	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29631_Pcan_Bot2011_IGS	HAYA57	1	BC29631_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29631	Sample	BC29631	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA57	IGS	45	BC29631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA57	CGTAATCCTCAGTACTGGCTA	149.3123
BC29631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25743	WGS84	BC29631	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29631_Sspa_Oph2007_COI	HAYA57	1	BC29631_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29631	Sample	BC29631	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA57	COI	45	BC29631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA57	GAAGGCCTTCCGATGATC	149.3123
BC29631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25743	WGS84	BC29631	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29631_Upin_Bot2011_COI	HAYA57	1	BC29631_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29631	Sample	BC29631	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA57	COI	45	BC29631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA57	CTTTAATTACAGCGTTTTTATTGTTGT	149.3123
BC29632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25737	WGS84	BC29632	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29632_Mgig_Bot2012_COI	HAYA56	1	BC29632_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29632	Sample	BC29632	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA56	COI	45	BC29632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA56	TCTTATTCGTTGGAGACTTTATAACCCT	149.31061
BC29633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25669	WGS84	BC29633	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29633_Cmae_Bot2011_COI	HAYA55	1	BC29633_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29633	Sample	BC29633	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA55	COI	45	BC29633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA55	ATGAACAGTCTATCCTCCTTTAG	149.31006
BC29633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25669	WGS84	BC29633	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29633_Msal_Bot2012_COI	HAYA55	1	BC29633_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29633	Sample	BC29633	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA55	COI	45	BC29633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA55	GYTAGTTCCRATGATGTTAGCTG	149.31006
BC29635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25792	WGS84	BC29635	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29635_Aamu_Bax2006_COI	HAYA53	1	BC29635_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29635	Sample	BC29635	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA53	COI	45	BC29635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA53	GCACAACCGGGATCTTTACTTC	149.30823
BC29635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25792	WGS84	BC29635	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29635_Pcan_Bot2011_IGS	HAYA53	1	BC29635_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29635	Sample	BC29635	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA53	IGS	45	BC29635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA53	CGTAATCCTCAGTACTGGCTA	149.30823
BC29635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25792	WGS84	BC29635	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29635_Sspa_Oph2007_COI	HAYA53	1	BC29635_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29635	Sample	BC29635	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA53	COI	45	BC29635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA53	GAAGGCCTTCCGATGATC	149.30823
BC29635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25792	WGS84	BC29635	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29635_Upin_Bot2011_COI	HAYA53	1	BC29635_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29635	Sample	BC29635	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA53	COI	45	BC29635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA53	CTTTAATTACAGCGTTTTTATTGTTGT	149.30823
BC29636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25735	WGS84	BC29636	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29636_Mgig_Bot2012_COI	HAYA52	1	BC29636_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29636	Sample	BC29636	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA52	COI	45	BC29636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA52	TCTTATTCGTTGGAGACTTTATAACCCT	149.31285
BC29637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25823	WGS84	BC29637	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29637_Cmae_Bot2011_COI	HAYA51	1	BC29637_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29637	Sample	BC29637	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA51	COI	45	BC29637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA51	ATGAACAGTCTATCCTCCTTTAG	149.31177
BC29637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25823	WGS84	BC29637	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29637_Msal_Bot2012_COI	HAYA51	1	BC29637_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29637	Sample	BC29637	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA51	COI	45	BC29637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA51	GYTAGTTCCRATGATGTTAGCTG	149.31177
BC29639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25811	WGS84	BC29639	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29639_Aamu_Bax2006_COI	HAYA49	1	BC29639_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29639	Sample	BC29639	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA49	COI	45	BC29639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA49	GCACAACCGGGATCTTTACTTC	149.31273
BC29639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25811	WGS84	BC29639	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29639_Pcan_Bot2011_IGS	HAYA49	1	BC29639_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29639	Sample	BC29639	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA49	IGS	45	BC29639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA49	CGTAATCCTCAGTACTGGCTA	149.31273
BC29639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25811	WGS84	BC29639	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29639_Sspa_Oph2007_COI	HAYA49	1	BC29639_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29639	Sample	BC29639	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA49	COI	45	BC29639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA49	GAAGGCCTTCCGATGATC	149.31273
BC29639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25811	WGS84	BC29639	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29639_Upin_Bot2011_COI	HAYA49	1	BC29639_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29639	Sample	BC29639	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA49	COI	45	BC29639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA49	CTTTAATTACAGCGTTTTTATTGTTGT	149.31273
BC29640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25897	WGS84	BC29640	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29640_Cmae_Bot2011_COI	HAYA48	1	BC29640_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29640	Sample	BC29640	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA48	COI	45	BC29640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA48	ATGAACAGTCTATCCTCCTTTAG	149.31222
BC29640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25897	WGS84	BC29640	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29640_Msal_Bot2012_COI	HAYA48	1	BC29640_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29640	Sample	BC29640	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA48	COI	45	BC29640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA48	GYTAGTTCCRATGATGTTAGCTG	149.31222
BC29642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25917	WGS84	BC29642	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29642_Aamu_Bax2006_COI	HAYA46	1	BC29642_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29642	Sample	BC29642	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA46	COI	45	BC29642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA46	GCACAACCGGGATCTTTACTTC	149.31302
BC29642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25917	WGS84	BC29642	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29642_Pcan_Bot2011_IGS	HAYA46	1	BC29642_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29642	Sample	BC29642	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA46	IGS	45	BC29642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA46	CGTAATCCTCAGTACTGGCTA	149.31302
BC29642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25917	WGS84	BC29642	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29642_Sspa_Oph2007_COI	HAYA46	1	BC29642_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29642	Sample	BC29642	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA46	COI	45	BC29642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA46	GAAGGCCTTCCGATGATC	149.31302
BC29642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25917	WGS84	BC29642	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29642_Upin_Bot2011_COI	HAYA46	1	BC29642_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29642	Sample	BC29642	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA46	COI	45	BC29642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA46	CTTTAATTACAGCGTTTTTATTGTTGT	149.31302
BC29643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25875	WGS84	BC29643	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29643_Mgig_Bot2012_COI	HAYA45	1	BC29643_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29643	Sample	BC29643	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA45	COI	45	BC29643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA45	TCTTATTCGTTGGAGACTTTATAACCCT	149.31316
BC29644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25974	WGS84	BC29644	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29644_Cmae_Bot2011_COI	HAYA44	1	BC29644_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29644	Sample	BC29644	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA44	COI	45	BC29644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA44	ATGAACAGTCTATCCTCCTTTAG	149.31053
BC29644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25974	WGS84	BC29644	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29644_Msal_Bot2012_COI	HAYA44	1	BC29644_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29644	Sample	BC29644	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA44	COI	45	BC29644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA44	GYTAGTTCCRATGATGTTAGCTG	149.31053
BC29646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25938	WGS84	BC29646	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29646_Aamu_Bax2006_COI	HAYA42	1	BC29646_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29646	Sample	BC29646	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA42	COI	45	BC29646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA42	GCACAACCGGGATCTTTACTTC	149.3134
BC29646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25938	WGS84	BC29646	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29646_Pcan_Bot2011_IGS	HAYA42	1	BC29646_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29646	Sample	BC29646	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA42	IGS	45	BC29646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA42	CGTAATCCTCAGTACTGGCTA	149.3134
BC29646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25938	WGS84	BC29646	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29646_Sspa_Oph2007_COI	HAYA42	1	BC29646_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29646	Sample	BC29646	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA42	COI	45	BC29646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA42	GAAGGCCTTCCGATGATC	149.3134
BC29646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25938	WGS84	BC29646	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29646_Upin_Bot2011_COI	HAYA42	1	BC29646_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29646	Sample	BC29646	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA42	COI	45	BC29646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA42	CTTTAATTACAGCGTTTTTATTGTTGT	149.3134
BC29647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26013	WGS84	BC29647	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29647_Mgig_Bot2012_COI	HAYA41	1	BC29647_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29647	Sample	BC29647	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA41	COI	45	BC29647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA41	TCTTATTCGTTGGAGACTTTATAACCCT	149.31281
BC29648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25966	WGS84	BC29648	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29648_Cmae_Bot2011_COI	HAYA40	1	BC29648_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29648	Sample	BC29648	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA40	COI	45	BC29648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA40	ATGAACAGTCTATCCTCCTTTAG	149.31048
BC29648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.25966	WGS84	BC29648	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29648_Msal_Bot2012_COI	HAYA40	1	BC29648_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29648	Sample	BC29648	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA40	COI	45	BC29648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA40	GYTAGTTCCRATGATGTTAGCTG	149.31048
BC29650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26105	WGS84	BC29650	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29650_Mgig_Bot2012_COI	HAYA38	1	BC29650_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29650	Sample	BC29650	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA38	COI	45	BC29650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA38	TCTTATTCGTTGGAGACTTTATAACCCT	149.31414
BC29651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26007	WGS84	BC29651	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29651_Cmae_Bot2011_COI	HAYA37	1	BC29651_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29651	Sample	BC29651	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA37	COI	45	BC29651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA37	ATGAACAGTCTATCCTCCTTTAG	149.31273
BC29651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26007	WGS84	BC29651	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29651_Msal_Bot2012_COI	HAYA37	1	BC29651_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29651	Sample	BC29651	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA37	COI	45	BC29651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA37	GYTAGTTCCRATGATGTTAGCTG	149.31273
BC29653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26043	WGS84	BC29653	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29653_Aamu_Bax2006_COI	HAYA35	1	BC29653_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29653	Sample	BC29653	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA35	COI	45	BC29653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA35	GCACAACCGGGATCTTTACTTC	149.30992
BC29653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26043	WGS84	BC29653	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29653_Pcan_Bot2011_IGS	HAYA35	1	BC29653_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29653	Sample	BC29653	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA35	IGS	45	BC29653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA35	CGTAATCCTCAGTACTGGCTA	149.30992
BC29653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26043	WGS84	BC29653	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29653_Sspa_Oph2007_COI	HAYA35	1	BC29653_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29653	Sample	BC29653	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA35	COI	45	BC29653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA35	GAAGGCCTTCCGATGATC	149.30992
BC29653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26043	WGS84	BC29653	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29653_Upin_Bot2011_COI	HAYA35	1	BC29653_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29653	Sample	BC29653	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA35	COI	45	BC29653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA35	CTTTAATTACAGCGTTTTTATTGTTGT	149.30992
BC29654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26084	WGS84	BC29654	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29654_Mgig_Bot2012_COI	HAYA34	1	BC29654_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29654	Sample	BC29654	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA34	COI	45	BC29654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA34	TCTTATTCGTTGGAGACTTTATAACCCT	149.31405
BC29655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26145	WGS84	BC29655	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29655_Cmae_Bot2011_COI	HAYA33	1	BC29655_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29655	Sample	BC29655	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA33	COI	45	BC29655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA33	ATGAACAGTCTATCCTCCTTTAG	149.31383
BC29655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26145	WGS84	BC29655	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29655_Msal_Bot2012_COI	HAYA33	1	BC29655_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29655	Sample	BC29655	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA33	COI	45	BC29655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA33	GYTAGTTCCRATGATGTTAGCTG	149.31383
BC29657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26186	WGS84	BC29657	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29657_Aamu_Bax2006_COI	HAYA31	1	BC29657_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29657	Sample	BC29657	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA31	COI	45	BC29657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA31	GCACAACCGGGATCTTTACTTC	149.3153
BC29657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26186	WGS84	BC29657	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29657_Pcan_Bot2011_IGS	HAYA31	1	BC29657_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29657	Sample	BC29657	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA31	IGS	45	BC29657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA31	CGTAATCCTCAGTACTGGCTA	149.3153
BC29657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26186	WGS84	BC29657	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29657_Sspa_Oph2007_COI	HAYA31	1	BC29657_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29657	Sample	BC29657	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA31	COI	45	BC29657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA31	GAAGGCCTTCCGATGATC	149.3153
BC29657	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26186	WGS84	BC29657	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29657_Upin_Bot2011_COI	HAYA31	1	BC29657_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29657	Sample	BC29657	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA31	COI	45	BC29657	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA31	CTTTAATTACAGCGTTTTTATTGTTGT	149.3153
BC29658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26249	WGS84	BC29658	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29658_Mgig_Bot2012_COI	HAYA30	1	BC29658_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29658	Sample	BC29658	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA30	COI	45	BC29658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA30	TCTTATTCGTTGGAGACTTTATAACCCT	149.31419
BC29659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26259	WGS84	BC29659	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29659_Cmae_Bot2011_COI	HAYA29	1	BC29659_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29659	Sample	BC29659	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA29	COI	45	BC29659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA29	ATGAACAGTCTATCCTCCTTTAG	149.31503
BC29659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26259	WGS84	BC29659	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29659_Msal_Bot2012_COI	HAYA29	1	BC29659_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29659	Sample	BC29659	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA29	COI	45	BC29659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA29	GYTAGTTCCRATGATGTTAGCTG	149.31503
BC29660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26306	WGS84	BC29660	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29660_Aamu_Bax2006_COI	HAYA28	1	BC29660_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29660	Sample	BC29660	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA28	COI	45	BC29660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA28	GCACAACCGGGATCTTTACTTC	149.31389
BC29660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26306	WGS84	BC29660	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29660_Pcan_Bot2011_IGS	HAYA28	1	BC29660_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29660	Sample	BC29660	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA28	IGS	45	BC29660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA28	CGTAATCCTCAGTACTGGCTA	149.31389
BC29660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26306	WGS84	BC29660	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29660_Sspa_Oph2007_COI	HAYA28	1	BC29660_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29660	Sample	BC29660	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA28	COI	45	BC29660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA28	GAAGGCCTTCCGATGATC	149.31389
BC29660	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26306	WGS84	BC29660	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29660_Upin_Bot2011_COI	HAYA28	1	BC29660_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29660	Sample	BC29660	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA28	COI	45	BC29660	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA28	CTTTAATTACAGCGTTTTTATTGTTGT	149.31389
BC29661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26326	WGS84	BC29661	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29661_Mgig_Bot2012_COI	HAYA27	1	BC29661_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29661	Sample	BC29661	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA27	COI	45	BC29661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA27	TCTTATTCGTTGGAGACTTTATAACCCT	149.31547
BC29662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26291	WGS84	BC29662	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29662_Cmae_Bot2011_COI	HAYA26	1	BC29662_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29662	Sample	BC29662	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA26	COI	45	BC29662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA26	ATGAACAGTCTATCCTCCTTTAG	149.31415
BC29662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26291	WGS84	BC29662	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29662_Msal_Bot2012_COI	HAYA26	1	BC29662_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29662	Sample	BC29662	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA26	COI	45	BC29662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA26	GYTAGTTCCRATGATGTTAGCTG	149.31415
BC29664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26401	WGS84	BC29664	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29664_Aamu_Bax2006_COI	HAYA24	1	BC29664_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29664	Sample	BC29664	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA24	COI	45	BC29664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA24	GCACAACCGGGATCTTTACTTC	149.31418
BC29664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26401	WGS84	BC29664	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29664_Pcan_Bot2011_IGS	HAYA24	1	BC29664_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29664	Sample	BC29664	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA24	IGS	45	BC29664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA24	CGTAATCCTCAGTACTGGCTA	149.31418
BC29664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26401	WGS84	BC29664	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29664_Sspa_Oph2007_COI	HAYA24	1	BC29664_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29664	Sample	BC29664	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA24	COI	45	BC29664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA24	GAAGGCCTTCCGATGATC	149.31418
BC29664	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26401	WGS84	BC29664	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29664_Upin_Bot2011_COI	HAYA24	1	BC29664_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29664	Sample	BC29664	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA24	COI	45	BC29664	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA24	CTTTAATTACAGCGTTTTTATTGTTGT	149.31418
BC29665	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26359	WGS84	BC29665	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29665_Mgig_Bot2012_COI	HAYA23	1	BC29665_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29665	Sample	BC29665	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA23	COI	45	BC29665	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA23	TCTTATTCGTTGGAGACTTTATAACCCT	149.31395
BC29666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26398	WGS84	BC29666	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29666_Cmae_Bot2011_COI	HAYA22	1	BC29666_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29666	Sample	BC29666	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA22	COI	45	BC29666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA22	ATGAACAGTCTATCCTCCTTTAG	149.31106
BC29666	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26398	WGS84	BC29666	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29666_Msal_Bot2012_COI	HAYA22	1	BC29666_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29666	Sample	BC29666	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA22	COI	45	BC29666	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA22	GYTAGTTCCRATGATGTTAGCTG	149.31106
BC29668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26436	WGS84	BC29668	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29668_Aamu_Bax2006_COI	HAYA20	1	BC29668_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29668	Sample	BC29668	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA20	COI	45	BC29668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA20	GCACAACCGGGATCTTTACTTC	149.31409
BC29668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26436	WGS84	BC29668	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29668_Pcan_Bot2011_IGS	HAYA20	1	BC29668_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29668	Sample	BC29668	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA20	IGS	45	BC29668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA20	CGTAATCCTCAGTACTGGCTA	149.31409
BC29668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26436	WGS84	BC29668	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29668_Sspa_Oph2007_COI	HAYA20	1	BC29668_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29668	Sample	BC29668	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA20	COI	45	BC29668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA20	GAAGGCCTTCCGATGATC	149.31409
BC29668	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26436	WGS84	BC29668	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29668_Upin_Bot2011_COI	HAYA20	1	BC29668_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29668	Sample	BC29668	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA20	COI	45	BC29668	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA20	CTTTAATTACAGCGTTTTTATTGTTGT	149.31409
BC29669	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26538	WGS84	BC29669	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29669_Mgig_Bot2012_COI	HAYA19	1	BC29669_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29669	Sample	BC29669	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA19	COI	45	BC29669	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA19	TCTTATTCGTTGGAGACTTTATAACCCT	149.3153
BC29671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26574	WGS84	BC29671	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29671_Aamu_Bax2006_COI	HAYA17	1	BC29671_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29671	Sample	BC29671	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA17	COI	45	BC29671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA17	GCACAACCGGGATCTTTACTTC	149.31447
BC29671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26574	WGS84	BC29671	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29671_Pcan_Bot2011_IGS	HAYA17	1	BC29671_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29671	Sample	BC29671	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA17	IGS	45	BC29671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA17	CGTAATCCTCAGTACTGGCTA	149.31447
BC29671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26574	WGS84	BC29671	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29671_Sspa_Oph2007_COI	HAYA17	1	BC29671_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29671	Sample	BC29671	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA17	COI	45	BC29671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA17	GAAGGCCTTCCGATGATC	149.31447
BC29671	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26574	WGS84	BC29671	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29671_Upin_Bot2011_COI	HAYA17	1	BC29671_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29671	Sample	BC29671	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA17	COI	45	BC29671	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA17	CTTTAATTACAGCGTTTTTATTGTTGT	149.31447
BC29672	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26587	WGS84	BC29672	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29672_Mgig_Bot2012_COI	HAYA16	1	BC29672_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29672	Sample	BC29672	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA16	COI	45	BC29672	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA16	TCTTATTCGTTGGAGACTTTATAACCCT	149.3118
BC29673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26654	WGS84	BC29673	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29673_Cmae_Bot2011_COI	HAYA15	1	BC29673_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29673	Sample	BC29673	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA15	COI	45	BC29673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA15	ATGAACAGTCTATCCTCCTTTAG	149.31624
BC29673	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26654	WGS84	BC29673	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29673_Msal_Bot2012_COI	HAYA15	1	BC29673_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29673	Sample	BC29673	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA15	COI	45	BC29673	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA15	GYTAGTTCCRATGATGTTAGCTG	149.31624
BC29675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29675	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29675_Aamu_Bax2006_COI	HAYA13	1	BC29675_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29675	Sample	BC29675	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA13	COI	45	BC29675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA13	GCACAACCGGGATCTTTACTTC	149.31476
BC29675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29675	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29675_Pcan_Bot2011_IGS	HAYA13	1	BC29675_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29675	Sample	BC29675	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA13	IGS	45	BC29675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA13	CGTAATCCTCAGTACTGGCTA	149.31476
BC29675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29675	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29675_Sspa_Oph2007_COI	HAYA13	1	BC29675_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29675	Sample	BC29675	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA13	COI	45	BC29675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA13	GAAGGCCTTCCGATGATC	149.31476
BC29675	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29675	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29675_Upin_Bot2011_COI	HAYA13	1	BC29675_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29675	Sample	BC29675	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA13	COI	45	BC29675	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA13	CTTTAATTACAGCGTTTTTATTGTTGT	149.31476
BC29676	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26672	WGS84	BC29676	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29676_Mgig_Bot2012_COI	HAYA12	1	BC29676_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29676	Sample	BC29676	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA12	COI	45	BC29676	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA12	TCTTATTCGTTGGAGACTTTATAACCCT	149.31633
BC29677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26725	WGS84	BC29677	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29677_Cmae_Bot2011_COI	HAYA11	1	BC29677_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29677	Sample	BC29677	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA11	COI	45	BC29677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA11	ATGAACAGTCTATCCTCCTTTAG	149.31629
BC29677	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26725	WGS84	BC29677	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29677_Msal_Bot2012_COI	HAYA11	1	BC29677_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29677	Sample	BC29677	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA11	COI	45	BC29677	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA11	GYTAGTTCCRATGATGTTAGCTG	149.31629
BC29679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2668	WGS84	BC29679	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29679_Aamu_Bax2006_COI	HAYA9	1	BC29679_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29679	Sample	BC29679	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA9	COI	45	BC29679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA9	GCACAACCGGGATCTTTACTTC	149.31294
BC29679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2668	WGS84	BC29679	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29679_Pcan_Bot2011_IGS	HAYA9	1	BC29679_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29679	Sample	BC29679	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA9	IGS	45	BC29679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA9	CGTAATCCTCAGTACTGGCTA	149.31294
BC29679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2668	WGS84	BC29679	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29679_Sspa_Oph2007_COI	HAYA9	1	BC29679_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29679	Sample	BC29679	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA9	COI	45	BC29679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA9	GAAGGCCTTCCGATGATC	149.31294
BC29679	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2668	WGS84	BC29679	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29679_Upin_Bot2011_COI	HAYA9	1	BC29679_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29679	Sample	BC29679	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA9	COI	45	BC29679	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA9	CTTTAATTACAGCGTTTTTATTGTTGT	149.31294
BC29680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26761	WGS84	BC29680	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29680_Cmae_Bot2011_COI	HAYA8	1	BC29680_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29680	Sample	BC29680	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA8	COI	45	BC29680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA8	ATGAACAGTCTATCCTCCTTTAG	149.31205
BC29680	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26761	WGS84	BC29680	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29680_Msal_Bot2012_COI	HAYA8	1	BC29680_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29680	Sample	BC29680	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA8	COI	45	BC29680	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA8	GYTAGTTCCRATGATGTTAGCTG	149.31205
BC29682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26714	WGS84	BC29682	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29682_Aamu_Bax2006_COI	HAYA6	1	BC29682_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29682	Sample	BC29682	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA6	COI	45	BC29682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA6	GCACAACCGGGATCTTTACTTC	149.31552
BC29682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26714	WGS84	BC29682	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29682_Pcan_Bot2011_IGS	HAYA6	1	BC29682_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29682	Sample	BC29682	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA6	IGS	45	BC29682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA6	CGTAATCCTCAGTACTGGCTA	149.31552
BC29682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26714	WGS84	BC29682	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29682_Sspa_Oph2007_COI	HAYA6	1	BC29682_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29682	Sample	BC29682	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA6	COI	45	BC29682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA6	GAAGGCCTTCCGATGATC	149.31552
BC29682	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26714	WGS84	BC29682	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29682_Upin_Bot2011_COI	HAYA6	1	BC29682_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29682	Sample	BC29682	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA6	COI	45	BC29682	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA6	CTTTAATTACAGCGTTTTTATTGTTGT	149.31552
BC29683	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.2681	WGS84	BC29683	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29683_Mgig_Bot2012_COI	HAYA5	1	BC29683_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29683	Sample	BC29683	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA5	COI	45	BC29683	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA5	TCTTATTCGTTGGAGACTTTATAACCCT	149.31408
BC29684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26749	WGS84	BC29684	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29684_Cmae_Bot2011_COI	HAYA4	1	BC29684_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29684	Sample	BC29684	targeted	Absent	Sample	Cmae_Bot2011_COI	HAYA4	COI	45	BC29684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA4	ATGAACAGTCTATCCTCCTTTAG	149.3128
BC29684	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26749	WGS84	BC29684	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29684_Msal_Bot2012_COI	HAYA4	1	BC29684_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29684	Sample	BC29684	targeted	Absent	Sample	Msal_Bot2012_COI	HAYA4	COI	45	BC29684	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA4	GYTAGTTCCRATGATGTTAGCTG	149.3128
BC29686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26894	WGS84	BC29686	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29686_Aamu_Bax2006_COI	HAYA2	1	BC29686_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29686	Sample	BC29686	targeted	Absent	Sample	Aamu_Bax2006_COI	HAYA2	COI	45	BC29686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA2	GCACAACCGGGATCTTTACTTC	149.31393
BC29686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26894	WGS84	BC29686	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29686_Pcan_Bot2011_IGS	HAYA2	1	BC29686_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29686	Sample	BC29686	targeted	Absent	Sample	Pcan_Bot2011_IGS	HAYA2	IGS	45	BC29686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA2	CGTAATCCTCAGTACTGGCTA	149.31393
BC29686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26894	WGS84	BC29686	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29686_Sspa_Oph2007_COI	HAYA2	1	BC29686_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29686	Sample	BC29686	targeted	Absent	Sample	Sspa_Oph2007_COI	HAYA2	COI	45	BC29686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA2	GAAGGCCTTCCGATGATC	149.31393
BC29686	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26894	WGS84	BC29686	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29686_Upin_Bot2011_COI	HAYA2	1	BC29686_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29686	Sample	BC29686	targeted	Absent	Sample	Upin_Bot2011_COI	HAYA2	COI	45	BC29686	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA2	CTTTAATTACAGCGTTTTTATTGTTGT	149.31393
BC29687	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12151	-21.26989	WGS84	BC29687	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Hay_Point_Summer	3/11/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29687_Mgig_Bot2012_COI	HAYA1	1	BC29687_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29687	Sample	BC29687	targeted	Absent	Sample	Mgig_Bot2012_COI	HAYA1	COI	45	BC29687	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	HAYA1	TCTTATTCGTTGGAGACTTTATAACCCT	149.31431
BC29688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18009	WGS84	BC29688	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.537	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29688_Cmae_Bot2011_COI	DEVA19	1	BC29688_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29688	Sample	BC29688	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA19	COI	45	BC29688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA19	ATGAACAGTCTATCCTCCTTTAG	146.3645
BC29688	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18009	WGS84	BC29688	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29688_Msal_Bot2012_COI	DEVA19	1	BC29688_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29688	Sample	BC29688	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA19	COI	45	BC29688	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA19	GYTAGTTCCRATGATGTTAGCTG	146.3645
BC29690	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18121	WGS84	BC29690	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.484	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29690_Mgig_Bot2012_COI	DEVA17	1	BC29690_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29690	Sample	BC29690	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA17	COI	45	BC29690	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA17	TCTTATTCGTTGGAGACTTTATAACCCT	146.36505
BC29693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1824	WGS84	BC29693	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29693_Aamu_Bax2006_COI	DEVA15	1	BC29693_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29693	Sample	BC29693	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA15	COI	45	BC29693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA15	GCACAACCGGGATCTTTACTTC	146.36585
BC29693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1824	WGS84	BC29693	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29693_Pcan_Bot2011_IGS	DEVA15	1	BC29693_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29693	Sample	BC29693	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA15	IGS	45	BC29693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA15	CGTAATCCTCAGTACTGGCTA	146.36585
BC29693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1824	WGS84	BC29693	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29693_Sspa_Oph2007_COI	DEVA15	1	BC29693_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29693	Sample	BC29693	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA15	COI	45	BC29693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA15	GAAGGCCTTCCGATGATC	146.36585
BC29693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.1824	WGS84	BC29693	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29693_Upin_Bot2011_COI	DEVA15	1	BC29693_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29693	Sample	BC29693	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA15	COI	45	BC29693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA15	CTTTAATTACAGCGTTTTTATTGTTGT	146.36585
BC29694	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18351	WGS84	BC29694	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.664	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29694_Mgig_Bot2012_COI	DEVA13	1	BC29694_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29694	Sample	BC29694	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA13	COI	45	BC29694	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA13	TCTTATTCGTTGGAGACTTTATAACCCT	146.36547
BC29695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18368	WGS84	BC29695	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	37.501	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29695_Cmae_Bot2011_COI	DEVA12	1	BC29695_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29695	Sample	BC29695	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA12	COI	45	BC29695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA12	ATGAACAGTCTATCCTCCTTTAG	146.36384
BC29695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18368	WGS84	BC29695	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29695_Msal_Bot2012_COI	DEVA12	1	BC29695_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29695	Sample	BC29695	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA12	COI	45	BC29695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA12	GYTAGTTCCRATGATGTTAGCTG	146.36384
BC29697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18569	WGS84	BC29697	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29697_Aamu_Bax2006_COI	DEVA9	1	BC29697_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29697	Sample	BC29697	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA9	COI	45	BC29697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA9	GCACAACCGGGATCTTTACTTC	146.36387
BC29697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18569	WGS84	BC29697	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29697_Pcan_Bot2011_IGS	DEVA9	1	BC29697_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29697	Sample	BC29697	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA9	IGS	45	BC29697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA9	CGTAATCCTCAGTACTGGCTA	146.36387
BC29697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18569	WGS84	BC29697	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29697_Sspa_Oph2007_COI	DEVA9	1	BC29697_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29697	Sample	BC29697	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA9	COI	45	BC29697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA9	GAAGGCCTTCCGATGATC	146.36387
BC29697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18569	WGS84	BC29697	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29697_Upin_Bot2011_COI	DEVA9	1	BC29697_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29697	Sample	BC29697	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA9	COI	45	BC29697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA9	CTTTAATTACAGCGTTTTTATTGTTGT	146.36387
BC29698	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18654	WGS84	BC29698	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.654	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29698_Mgig_Bot2012_COI	DEVA8	1	BC29698_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29698	Sample	BC29698	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA8	COI	45	BC29698	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA8	TCTTATTCGTTGGAGACTTTATAACCCT	146.36659
BC29699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18728	WGS84	BC29699	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.629	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29699_Cmae_Bot2011_COI	DEVA6	1	BC29699_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29699	Sample	BC29699	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA6	COI	45	BC29699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA6	ATGAACAGTCTATCCTCCTTTAG	146.36737
BC29699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18728	WGS84	BC29699	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29699_Msal_Bot2012_COI	DEVA6	1	BC29699_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29699	Sample	BC29699	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA6	COI	45	BC29699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA6	GYTAGTTCCRATGATGTTAGCTG	146.36737
BC29701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18884	WGS84	BC29701	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29701_Aamu_Bax2006_COI	DEVA4	1	BC29701_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29701	Sample	BC29701	targeted	Absent	Sample	Aamu_Bax2006_COI	DEVA4	COI	45	BC29701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA4	GCACAACCGGGATCTTTACTTC	146.36796
BC29701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18884	WGS84	BC29701	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29701_Pcan_Bot2011_IGS	DEVA4	1	BC29701_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29701	Sample	BC29701	targeted	Absent	Sample	Pcan_Bot2011_IGS	DEVA4	IGS	45	BC29701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA4	CGTAATCCTCAGTACTGGCTA	146.36796
BC29701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18884	WGS84	BC29701	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29701_Sspa_Oph2007_COI	DEVA4	1	BC29701_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29701	Sample	BC29701	targeted	Absent	Sample	Sspa_Oph2007_COI	DEVA4	COI	45	BC29701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA4	GAAGGCCTTCCGATGATC	146.36796
BC29701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18884	WGS84	BC29701	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29701_Upin_Bot2011_COI	DEVA4	1	BC29701_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29701	Sample	BC29701	targeted	Absent	Sample	Upin_Bot2011_COI	DEVA4	COI	45	BC29701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA4	CTTTAATTACAGCGTTTTTATTGTTGT	146.36796
BC29702	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.18922	WGS84	BC29702	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.123	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29702_Mgig_Bot2012_COI	DEVA3	1	BC29702_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29702	Sample	BC29702	targeted	Present	Sample	Mgig_Bot2012_COI	DEVA3	COI	45	BC29702	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA3	TCTTATTCGTTGGAGACTTTATAACCCT	146.3675
BC29703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.19141	WGS84	BC29703	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.095	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29703_Cmae_Bot2011_COI	DEVA1	1	BC29703_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29703	Sample	BC29703	targeted	Present	Sample	Cmae_Bot2011_COI	DEVA1	COI	45	BC29703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA1	ATGAACAGTCTATCCTCCTTTAG	146.36788
BC29703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-41.19141	WGS84	BC29703	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Devonport_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29703_Msal_Bot2012_COI	DEVA1	1	BC29703_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29703	Sample	BC29703	targeted	Absent	Sample	Msal_Bot2012_COI	DEVA1	COI	45	BC29703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DEVA1	GYTAGTTCCRATGATGTTAGCTG	146.36788
BC29705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88231	WGS84	BC29705	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29705_Aamu_Bax2006_COI	NEWA44	1	BC29705_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29705	Sample	BC29705	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA44	COI	45	BC29705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA44	GCACAACCGGGATCTTTACTTC	151.75221
BC29705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88231	WGS84	BC29705	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29705_Pcan_Bot2011_IGS	NEWA44	1	BC29705_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29705	Sample	BC29705	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA44	IGS	45	BC29705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA44	CGTAATCCTCAGTACTGGCTA	151.75221
BC29705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88231	WGS84	BC29705	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29705_Sspa_Oph2007_COI	NEWA44	1	BC29705_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29705	Sample	BC29705	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA44	COI	45	BC29705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA44	GAAGGCCTTCCGATGATC	151.75221
BC29705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88231	WGS84	BC29705	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29705_Upin_Bot2011_COI	NEWA44	1	BC29705_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29705	Sample	BC29705	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA44	COI	45	BC29705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA44	CTTTAATTACAGCGTTTTTATTGTTGT	151.75221
BC29706	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88377	WGS84	BC29706	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.37	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29706_Mgig_Bot2012_COI	NEWA43	1	BC29706_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29706	Sample	BC29706	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA43	COI	45	BC29706	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA43	TCTTATTCGTTGGAGACTTTATAACCCT	151.75584
BC29707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88398	WGS84	BC29707	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29707_Cmae_Bot2011_COI	NEWA42	1	BC29707_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29707	Sample	BC29707	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA42	COI	45	BC29707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA42	ATGAACAGTCTATCCTCCTTTAG	151.75356
BC29707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88398	WGS84	BC29707	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29707_Msal_Bot2012_COI	NEWA42	1	BC29707_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29707	Sample	BC29707	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA42	COI	45	BC29707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA42	GYTAGTTCCRATGATGTTAGCTG	151.75356
BC29709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88524	WGS84	BC29709	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29709_Aamu_Bax2006_COI	NEWA40	1	BC29709_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29709	Sample	BC29709	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA40	COI	45	BC29709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA40	GCACAACCGGGATCTTTACTTC	151.7552
BC29709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88524	WGS84	BC29709	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29709_Pcan_Bot2011_IGS	NEWA40	1	BC29709_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29709	Sample	BC29709	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA40	IGS	45	BC29709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA40	CGTAATCCTCAGTACTGGCTA	151.7552
BC29709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88524	WGS84	BC29709	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29709_Sspa_Oph2007_COI	NEWA40	1	BC29709_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29709	Sample	BC29709	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA40	COI	45	BC29709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA40	GAAGGCCTTCCGATGATC	151.7552
BC29709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88524	WGS84	BC29709	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29709_Upin_Bot2011_COI	NEWA40	1	BC29709_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29709	Sample	BC29709	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA40	COI	45	BC29709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA40	CTTTAATTACAGCGTTTTTATTGTTGT	151.7552
BC29710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88718	WGS84	BC29710	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29710_Cmae_Bot2011_COI	NEWA39	1	BC29710_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29710	Sample	BC29710	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA39	COI	45	BC29710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA39	ATGAACAGTCTATCCTCCTTTAG	151.76167
BC29710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88718	WGS84	BC29710	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29710_Msal_Bot2012_COI	NEWA39	1	BC29710_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29710	Sample	BC29710	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA39	COI	45	BC29710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA39	GYTAGTTCCRATGATGTTAGCTG	151.76167
BC29712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88826	WGS84	BC29712	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29712_Aamu_Bax2006_COI	NEWA37	1	BC29712_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29712	Sample	BC29712	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA37	COI	45	BC29712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA37	GCACAACCGGGATCTTTACTTC	151.76569
BC29712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88826	WGS84	BC29712	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29712_Pcan_Bot2011_IGS	NEWA37	1	BC29712_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29712	Sample	BC29712	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA37	IGS	45	BC29712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA37	CGTAATCCTCAGTACTGGCTA	151.76569
BC29712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88826	WGS84	BC29712	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29712_Sspa_Oph2007_COI	NEWA37	1	BC29712_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29712	Sample	BC29712	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA37	COI	45	BC29712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA37	GAAGGCCTTCCGATGATC	151.76569
BC29712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88826	WGS84	BC29712	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29712_Upin_Bot2011_COI	NEWA37	1	BC29712_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29712	Sample	BC29712	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA37	COI	45	BC29712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA37	CTTTAATTACAGCGTTTTTATTGTTGT	151.76569
BC29713	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8887	WGS84	BC29713	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.677	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29713_Mgig_Bot2012_COI	NEWA36	1	BC29713_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29713	Sample	BC29713	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA36	COI	45	BC29713	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA36	TCTTATTCGTTGGAGACTTTATAACCCT	151.76318
BC29714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88836	WGS84	BC29714	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29714_Cmae_Bot2011_COI	NEWA35	1	BC29714_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29714	Sample	BC29714	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA35	COI	45	BC29714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA35	ATGAACAGTCTATCCTCCTTTAG	151.76012
BC29714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88836	WGS84	BC29714	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29714_Msal_Bot2012_COI	NEWA35	1	BC29714_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29714	Sample	BC29714	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA35	COI	45	BC29714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA35	GYTAGTTCCRATGATGTTAGCTG	151.76012
BC29716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88997	WGS84	BC29716	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29716_Aamu_Bax2006_COI	NEWA33	1	BC29716_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29716	Sample	BC29716	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA33	COI	45	BC29716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA33	GCACAACCGGGATCTTTACTTC	151.7669
BC29716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88997	WGS84	BC29716	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29716_Pcan_Bot2011_IGS	NEWA33	1	BC29716_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29716	Sample	BC29716	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA33	IGS	45	BC29716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA33	CGTAATCCTCAGTACTGGCTA	151.7669
BC29716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88997	WGS84	BC29716	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29716_Sspa_Oph2007_COI	NEWA33	1	BC29716_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29716	Sample	BC29716	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA33	COI	45	BC29716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA33	GAAGGCCTTCCGATGATC	151.7669
BC29716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.88997	WGS84	BC29716	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29716_Upin_Bot2011_COI	NEWA33	1	BC29716_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29716	Sample	BC29716	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA33	COI	45	BC29716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA33	CTTTAATTACAGCGTTTTTATTGTTGT	151.7669
BC29717	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.8901	WGS84	BC29717	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.631	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29717_Mgig_Bot2012_COI	NEWA32	1	BC29717_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29717	Sample	BC29717	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA32	COI	45	BC29717	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA32	TCTTATTCGTTGGAGACTTTATAACCCT	151.76482
BC29718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89009	WGS84	BC29718	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29718_Cmae_Bot2011_COI	NEWA31	1	BC29718_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29718	Sample	BC29718	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA31	COI	45	BC29718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA31	ATGAACAGTCTATCCTCCTTTAG	151.77235
BC29718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89009	WGS84	BC29718	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29718_Msal_Bot2012_COI	NEWA31	1	BC29718_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29718	Sample	BC29718	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA31	COI	45	BC29718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA31	GYTAGTTCCRATGATGTTAGCTG	151.77235
BC29720	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89121	WGS84	BC29720	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.578	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29720_Mgig_Bot2012_COI	NEWA29	1	BC29720_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29720	Sample	BC29720	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA29	COI	45	BC29720	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA29	TCTTATTCGTTGGAGACTTTATAACCCT	151.76873
BC29721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89171	WGS84	BC29721	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29721_Cmae_Bot2011_COI	NEWA28	1	BC29721_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29721	Sample	BC29721	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA28	COI	45	BC29721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA28	ATGAACAGTCTATCCTCCTTTAG	151.76605
BC29721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89171	WGS84	BC29721	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29721_Msal_Bot2012_COI	NEWA28	1	BC29721_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29721	Sample	BC29721	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA28	COI	45	BC29721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA28	GYTAGTTCCRATGATGTTAGCTG	151.76605
BC29723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89279	WGS84	BC29723	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29723_Aamu_Bax2006_COI	NEWA26	1	BC29723_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29723	Sample	BC29723	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA26	COI	45	BC29723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA26	GCACAACCGGGATCTTTACTTC	151.77002
BC29723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89279	WGS84	BC29723	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29723_Pcan_Bot2011_IGS	NEWA26	1	BC29723_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29723	Sample	BC29723	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA26	IGS	45	BC29723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA26	CGTAATCCTCAGTACTGGCTA	151.77002
BC29723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89279	WGS84	BC29723	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29723_Sspa_Oph2007_COI	NEWA26	1	BC29723_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29723	Sample	BC29723	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA26	COI	45	BC29723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA26	GAAGGCCTTCCGATGATC	151.77002
BC29723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89279	WGS84	BC29723	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29723_Upin_Bot2011_COI	NEWA26	1	BC29723_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29723	Sample	BC29723	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA26	COI	45	BC29723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA26	CTTTAATTACAGCGTTTTTATTGTTGT	151.77002
BC29724	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89331	WGS84	BC29724	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.576	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29724_Mgig_Bot2012_COI	NEWA25	1	BC29724_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29724	Sample	BC29724	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA25	COI	45	BC29724	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA25	TCTTATTCGTTGGAGACTTTATAACCCT	151.76761
BC29725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89442	WGS84	BC29725	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29725_Cmae_Bot2011_COI	NEWA24	1	BC29725_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29725	Sample	BC29725	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA24	COI	45	BC29725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA24	ATGAACAGTCTATCCTCCTTTAG	151.77146
BC29725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89442	WGS84	BC29725	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29725_Msal_Bot2012_COI	NEWA24	1	BC29725_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29725	Sample	BC29725	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA24	COI	45	BC29725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA24	GYTAGTTCCRATGATGTTAGCTG	151.77146
BC29727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89627	WGS84	BC29727	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29727_Aamu_Bax2006_COI	NEWA22	1	BC29727_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29727	Sample	BC29727	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA22	COI	45	BC29727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA22	GCACAACCGGGATCTTTACTTC	151.77242
BC29727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89627	WGS84	BC29727	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29727_Pcan_Bot2011_IGS	NEWA22	1	BC29727_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29727	Sample	BC29727	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA22	IGS	45	BC29727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA22	CGTAATCCTCAGTACTGGCTA	151.77242
BC29727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89627	WGS84	BC29727	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29727_Sspa_Oph2007_COI	NEWA22	1	BC29727_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29727	Sample	BC29727	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA22	COI	45	BC29727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA22	GAAGGCCTTCCGATGATC	151.77242
BC29727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89627	WGS84	BC29727	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29727_Upin_Bot2011_COI	NEWA22	1	BC29727_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29727	Sample	BC29727	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA22	COI	45	BC29727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA22	CTTTAATTACAGCGTTTTTATTGTTGT	151.77242
BC29728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89642	WGS84	BC29728	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.71	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29728_Mgig_Bot2012_COI	NEWA21	1	BC29728_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29728	Sample	BC29728	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA21	COI	45	BC29728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA21	TCTTATTCGTTGGAGACTTTATAACCCT	151.7701
BC29729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91159	WGS84	BC29729	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29729_Cmae_Bot2011_COI	NEWA20	1	BC29729_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29729	Sample	BC29729	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA20	COI	45	BC29729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA20	ATGAACAGTCTATCCTCCTTTAG	151.77401
BC29729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91159	WGS84	BC29729	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29729_Msal_Bot2012_COI	NEWA20	1	BC29729_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29729	Sample	BC29729	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA20	COI	45	BC29729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA20	GYTAGTTCCRATGATGTTAGCTG	151.77401
BC29730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91424	WGS84	BC29730	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29730_Aamu_Bax2006_COI	NEWA19	1	BC29730_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29730	Sample	BC29730	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA19	COI	45	BC29730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA19	GCACAACCGGGATCTTTACTTC	151.77313
BC29730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91424	WGS84	BC29730	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29730_Pcan_Bot2011_IGS	NEWA19	1	BC29730_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29730	Sample	BC29730	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA19	IGS	45	BC29730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA19	CGTAATCCTCAGTACTGGCTA	151.77313
BC29730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91424	WGS84	BC29730	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29730_Sspa_Oph2007_COI	NEWA19	1	BC29730_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29730	Sample	BC29730	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA19	COI	45	BC29730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA19	GAAGGCCTTCCGATGATC	151.77313
BC29730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91424	WGS84	BC29730	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29730_Upin_Bot2011_COI	NEWA19	1	BC29730_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29730	Sample	BC29730	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA19	COI	45	BC29730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA19	CTTTAATTACAGCGTTTTTATTGTTGT	151.77313
BC29731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91522	WGS84	BC29731	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.493	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29731_Mgig_Bot2012_COI	NEWA18	1	BC29731_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29731	Sample	BC29731	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA18	COI	45	BC29731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA18	TCTTATTCGTTGGAGACTTTATAACCCT	151.77531
BC29732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91678	WGS84	BC29732	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29732_Cmae_Bot2011_COI	NEWA17	1	BC29732_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29732	Sample	BC29732	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA17	COI	45	BC29732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA17	ATGAACAGTCTATCCTCCTTTAG	151.77668
BC29732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91678	WGS84	BC29732	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29732_Msal_Bot2012_COI	NEWA17	1	BC29732_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29732	Sample	BC29732	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA17	COI	45	BC29732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA17	GYTAGTTCCRATGATGTTAGCTG	151.77668
BC29734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91799	WGS84	BC29734	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29734_Aamu_Bax2006_COI	NEWA15	1	BC29734_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29734	Sample	BC29734	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA15	COI	45	BC29734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA15	GCACAACCGGGATCTTTACTTC	151.77576
BC29734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91799	WGS84	BC29734	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29734_Pcan_Bot2011_IGS	NEWA15	1	BC29734_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29734	Sample	BC29734	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA15	IGS	45	BC29734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA15	CGTAATCCTCAGTACTGGCTA	151.77576
BC29734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91799	WGS84	BC29734	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29734_Sspa_Oph2007_COI	NEWA15	1	BC29734_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29734	Sample	BC29734	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA15	COI	45	BC29734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA15	GAAGGCCTTCCGATGATC	151.77576
BC29734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91799	WGS84	BC29734	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29734_Upin_Bot2011_COI	NEWA15	1	BC29734_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29734	Sample	BC29734	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA15	COI	45	BC29734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA15	CTTTAATTACAGCGTTTTTATTGTTGT	151.77576
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29735_Cjap_Sim2018_COI	NEWA14	1	BC29735_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29735_Esin_And2018_Cyb	NEWA14	1	BC29735_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA14	Cytb	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	ACCCCTCCTCATATCCAACCA	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.387	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29735_Mgig_Bot2012_COI	NEWA14	1	BC29735_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	TCTTATTCGTTGGAGACTTTATAACCCT	151.777
BC29735	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.91987	WGS84	BC29735	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/07/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29735_Mjap_Gib2022_COI	NEWA14	1	BC29735_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29735	Sample	BC29735	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA14	COI	45	BC29735	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA14	AATTTAATTGTGACTAGGCATGGTCTG	151.777
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29736_Cmae_Bot2011_COI	NEWA13	1	BC29736_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	ATGAACAGTCTATCCTCCTTTAG	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29736_Hsan_Wil2021_COI	NEWA13	1	BC29736_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	CCTGGGCCGGTATAGTAGGT	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29736_Mros_Gib2022_COI	NEWA13	1	BC29736_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29736_Msal_Bot2012_COI	NEWA13	1	BC29736_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	GYTAGTTCCRATGATGTTAGCTG	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29736_Pvir_Dia2013_COI	NEWA13	1	BC29736_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	CTTAGTGGCATTAATTCGDAATCC	151.77052
BC29736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.89959	WGS84	BC29736	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29736_Rhar_Sim2018_COI	NEWA13	1	BC29736_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29736	Sample	BC29736	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA13	COI	45	BC29736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA13	CTGCTGCTATTGCTCATG	151.77052
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29738_Aamu_Bax2006_COI	NEWA11	1	BC29738_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	GCACAACCGGGATCTTTACTTC	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29738_Pcan_Bot2011_IGS	NEWA11	1	BC29738_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA11	IGS	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	CGTAATCCTCAGTACTGGCTA	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29738_Pper_Dia2013_COI	NEWA11	1	BC29738_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	CTTAGTGGCATTAATTCGDAATCC	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29738_Sspa_Oph2007_COI	NEWA11	1	BC29738_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	GAAGGCCTTCCGATGATC	151.77323
BC29738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90283	WGS84	BC29738	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29738_Upin_Bot2011_COI	NEWA11	1	BC29738_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29738	Sample	BC29738	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA11	COI	45	BC29738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA11	CTTTAATTACAGCGTTTTTATTGTTGT	151.77323
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29739_Cjap_Sim2018_COI	NEWA10	1	BC29739_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29739_Esin_And2018_Cyb	NEWA10	1	BC29739_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA10	Cytb	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	ACCCCTCCTCATATCCAACCA	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.398	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29739_Mgig_Bot2012_COI	NEWA10	1	BC29739_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	TCTTATTCGTTGGAGACTTTATAACCCT	151.77133
BC29739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90318	WGS84	BC29739	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29739_Mjap_Gib2022_COI	NEWA10	1	BC29739_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29739	Sample	BC29739	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA10	COI	45	BC29739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA10	AATTTAATTGTGACTAGGCATGGTCTG	151.77133
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29741_Aamu_Bax2006_COI	NEWA8	1	BC29741_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	GCACAACCGGGATCTTTACTTC	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29741_Pcan_Bot2011_IGS	NEWA8	1	BC29741_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA8	IGS	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	CGTAATCCTCAGTACTGGCTA	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29741_Pper_Dia2013_COI	NEWA8	1	BC29741_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	CTTAGTGGCATTAATTCGDAATCC	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29741_Sspa_Oph2007_COI	NEWA8	1	BC29741_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	GAAGGCCTTCCGATGATC	151.77154
BC29741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.90652	WGS84	BC29741	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29741_Upin_Bot2011_COI	NEWA8	1	BC29741_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29741	Sample	BC29741	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA8	COI	45	BC29741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA8	CTTTAATTACAGCGTTTTTATTGTTGT	151.77154
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29742_Cjap_Sim2018_COI	NEWA7	1	BC29742_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29742_Esin_And2018_Cyb	NEWA7	1	BC29742_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA7	Cytb	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	ACCCCTCCTCATATCCAACCA	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.186	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29742_Mgig_Bot2012_COI	NEWA7	1	BC29742_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	TCTTATTCGTTGGAGACTTTATAACCCT	151.76974
BC29742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92025	WGS84	BC29742	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29742_Mjap_Gib2022_COI	NEWA7	1	BC29742_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29742	Sample	BC29742	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA7	COI	45	BC29742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA7	AATTTAATTGTGACTAGGCATGGTCTG	151.76974
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29743_Cmae_Bot2011_COI	NEWA6	1	BC29743_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	ATGAACAGTCTATCCTCCTTTAG	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29743_Hsan_Wil2021_COI	NEWA6	1	BC29743_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	CCTGGGCCGGTATAGTAGGT	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29743_Mros_Gib2022_COI	NEWA6	1	BC29743_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29743_Msal_Bot2012_COI	NEWA6	1	BC29743_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	GYTAGTTCCRATGATGTTAGCTG	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29743_Pvir_Dia2013_COI	NEWA6	1	BC29743_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	CTTAGTGGCATTAATTCGDAATCC	151.77058
BC29743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92192	WGS84	BC29743	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29743_Rhar_Sim2018_COI	NEWA6	1	BC29743_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29743	Sample	BC29743	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA6	COI	45	BC29743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA6	CTGCTGCTATTGCTCATG	151.77058
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29745_Aamu_Bax2006_COI	NEWA4	1	BC29745_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	GCACAACCGGGATCTTTACTTC	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29745_Pcan_Bot2011_IGS	NEWA4	1	BC29745_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWA4	IGS	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	CGTAATCCTCAGTACTGGCTA	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29745_Pper_Dia2013_COI	NEWA4	1	BC29745_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Pper_Dia2013_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	CTTAGTGGCATTAATTCGDAATCC	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29745_Sspa_Oph2007_COI	NEWA4	1	BC29745_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	GAAGGCCTTCCGATGATC	151.77353
BC29745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92345	WGS84	BC29745	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29745_Upin_Bot2011_COI	NEWA4	1	BC29745_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29745	Sample	BC29745	targeted	Absent	Sample	Upin_Bot2011_COI	NEWA4	COI	45	BC29745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA4	CTTTAATTACAGCGTTTTTATTGTTGT	151.77353
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29746_Cjap_Sim2018_COI	NEWA3	1	BC29746_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Cjap_Sim2018_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29746_Esin_And2018_Cyb	NEWA3	1	BC29746_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Esin_And2018_Cyb	NEWA3	Cytb	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	ACCCCTCCTCATATCCAACCA	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.695	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29746_Mgig_Bot2012_COI	NEWA3	1	BC29746_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Present	Sample	Mgig_Bot2012_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	TCTTATTCGTTGGAGACTTTATAACCCT	151.77067
BC29746	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.92412	WGS84	BC29746	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29746_Mjap_Gib2022_COI	NEWA3	1	BC29746_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29746	Sample	BC29746	targeted	Absent	Sample	Mjap_Gib2022_COI	NEWA3	COI	45	BC29746	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA3	AATTTAATTGTGACTAGGCATGGTCTG	151.77067
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29747_Cmae_Bot2011_COI	NEWA2	1	BC29747_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	ATGAACAGTCTATCCTCCTTTAG	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29747_Hsan_Wil2021_COI	NEWA2	1	BC29747_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Hsan_Wil2021_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	CCTGGGCCGGTATAGTAGGT	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29747_Mros_Gib2022_COI	NEWA2	1	BC29747_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Mros_Gib2022_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29747_Msal_Bot2012_COI	NEWA2	1	BC29747_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Msal_Bot2012_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	GYTAGTTCCRATGATGTTAGCTG	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29747_Pvir_Dia2013_COI	NEWA2	1	BC29747_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Pvir_Dia2013_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	CTTAGTGGCATTAATTCGDAATCC	151.76782
BC29747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12145	-32.9244	WGS84	BC29747	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Newcastle_Summer	3/06/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29747_Rhar_Sim2018_COI	NEWA2	1	BC29747_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29747	Sample	BC29747	targeted	Absent	Sample	Rhar_Sim2018_COI	NEWA2	COI	45	BC29747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWA2	CTGCTGCTATTGCTCATG	151.76782
BC29749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4533	WGS84	BC29749	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29749_Aamu_Bax2006_COI	KEMA35	1	BC29749_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29749	Sample	BC29749	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA35	COI	45	BC29749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA35	GCACAACCGGGATCTTTACTTC	150.8953
BC29749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4533	WGS84	BC29749	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29749_Pcan_Bot2011_IGS	KEMA35	1	BC29749_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29749	Sample	BC29749	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA35	IGS	45	BC29749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA35	CGTAATCCTCAGTACTGGCTA	150.8953
BC29749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4533	WGS84	BC29749	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29749_Sspa_Oph2007_COI	KEMA35	1	BC29749_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29749	Sample	BC29749	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA35	COI	45	BC29749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA35	GAAGGCCTTCCGATGATC	150.8953
BC29749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4533	WGS84	BC29749	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29749_Upin_Bot2011_COI	KEMA35	1	BC29749_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29749	Sample	BC29749	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA35	COI	45	BC29749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA35	CTTTAATTACAGCGTTTTTATTGTTGT	150.8953
BC29750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45408	WGS84	BC29750	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29750_Cmae_Bot2011_COI	KEMA34	1	BC29750_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29750	Sample	BC29750	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA34	COI	45	BC29750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA34	ATGAACAGTCTATCCTCCTTTAG	150.89427
BC29750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45408	WGS84	BC29750	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29750_Msal_Bot2012_COI	KEMA34	1	BC29750_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29750	Sample	BC29750	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA34	COI	45	BC29750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA34	GYTAGTTCCRATGATGTTAGCTG	150.89427
BC29752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45566	WGS84	BC29752	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29752_Aamu_Bax2006_COI	KEMA32	1	BC29752_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29752	Sample	BC29752	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA32	COI	45	BC29752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA32	GCACAACCGGGATCTTTACTTC	150.89579
BC29752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45566	WGS84	BC29752	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29752_Pcan_Bot2011_IGS	KEMA32	1	BC29752_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29752	Sample	BC29752	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA32	IGS	45	BC29752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA32	CGTAATCCTCAGTACTGGCTA	150.89579
BC29752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45566	WGS84	BC29752	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29752_Sspa_Oph2007_COI	KEMA32	1	BC29752_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29752	Sample	BC29752	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA32	COI	45	BC29752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA32	GAAGGCCTTCCGATGATC	150.89579
BC29752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45566	WGS84	BC29752	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29752_Upin_Bot2011_COI	KEMA32	1	BC29752_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29752	Sample	BC29752	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA32	COI	45	BC29752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA32	CTTTAATTACAGCGTTTTTATTGTTGT	150.89579
BC29753	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45621	WGS84	BC29753	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.482	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29753_Mgig_Bot2012_COI	KEMA31	1	BC29753_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29753	Sample	BC29753	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA31	COI	45	BC29753	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA31	TCTTATTCGTTGGAGACTTTATAACCCT	150.89105
BC29754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45643	WGS84	BC29754	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29754_Cmae_Bot2011_COI	KEMA30	1	BC29754_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29754	Sample	BC29754	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA30	COI	45	BC29754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA30	ATGAACAGTCTATCCTCCTTTAG	150.88892
BC29754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45643	WGS84	BC29754	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29754_Msal_Bot2012_COI	KEMA30	1	BC29754_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29754	Sample	BC29754	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA30	COI	45	BC29754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA30	GYTAGTTCCRATGATGTTAGCTG	150.88892
BC29756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45774	WGS84	BC29756	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29756_Aamu_Bax2006_COI	KEMA28	1	BC29756_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29756	Sample	BC29756	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA28	COI	45	BC29756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA28	GCACAACCGGGATCTTTACTTC	150.89314
BC29756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45774	WGS84	BC29756	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29756_Pcan_Bot2011_IGS	KEMA28	1	BC29756_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29756	Sample	BC29756	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA28	IGS	45	BC29756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA28	CGTAATCCTCAGTACTGGCTA	150.89314
BC29756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45774	WGS84	BC29756	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29756_Sspa_Oph2007_COI	KEMA28	1	BC29756_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29756	Sample	BC29756	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA28	COI	45	BC29756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA28	GAAGGCCTTCCGATGATC	150.89314
BC29756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45774	WGS84	BC29756	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29756_Upin_Bot2011_COI	KEMA28	1	BC29756_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29756	Sample	BC29756	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA28	COI	45	BC29756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA28	CTTTAATTACAGCGTTTTTATTGTTGT	150.89314
BC29757	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45868	WGS84	BC29757	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.087	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29757_Mgig_Bot2012_COI	KEMA27	1	BC29757_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29757	Sample	BC29757	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA27	COI	45	BC29757	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA27	TCTTATTCGTTGGAGACTTTATAACCCT	150.89076
BC29758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45976	WGS84	BC29758	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29758_Cmae_Bot2011_COI	KEMA26	1	BC29758_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29758	Sample	BC29758	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA26	COI	45	BC29758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA26	ATGAACAGTCTATCCTCCTTTAG	150.89679
BC29758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45976	WGS84	BC29758	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29758_Msal_Bot2012_COI	KEMA26	1	BC29758_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29758	Sample	BC29758	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA26	COI	45	BC29758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA26	GYTAGTTCCRATGATGTTAGCTG	150.89679
BC29760	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.45994	WGS84	BC29760	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.353	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29760_Mgig_Bot2012_COI	KEMA24	1	BC29760_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29760	Sample	BC29760	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA24	COI	45	BC29760	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA24	TCTTATTCGTTGGAGACTTTATAACCCT	150.8924
BC29761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4613	WGS84	BC29761	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29761_Cmae_Bot2011_COI	KEMA23	1	BC29761_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29761	Sample	BC29761	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA23	COI	45	BC29761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA23	ATGAACAGTCTATCCTCCTTTAG	150.89796
BC29761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4613	WGS84	BC29761	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29761_Msal_Bot2012_COI	KEMA23	1	BC29761_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29761	Sample	BC29761	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA23	COI	45	BC29761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA23	GYTAGTTCCRATGATGTTAGCTG	150.89796
BC29763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46143	WGS84	BC29763	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29763_Aamu_Bax2006_COI	KEMA21	1	BC29763_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29763	Sample	BC29763	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA21	COI	45	BC29763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA21	GCACAACCGGGATCTTTACTTC	150.89383
BC29763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46143	WGS84	BC29763	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29763_Pcan_Bot2011_IGS	KEMA21	1	BC29763_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29763	Sample	BC29763	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA21	IGS	45	BC29763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA21	CGTAATCCTCAGTACTGGCTA	150.89383
BC29763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46143	WGS84	BC29763	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29763_Sspa_Oph2007_COI	KEMA21	1	BC29763_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29763	Sample	BC29763	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA21	COI	45	BC29763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA21	GAAGGCCTTCCGATGATC	150.89383
BC29763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46143	WGS84	BC29763	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29763_Upin_Bot2011_COI	KEMA21	1	BC29763_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29763	Sample	BC29763	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA21	COI	45	BC29763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA21	CTTTAATTACAGCGTTTTTATTGTTGT	150.89383
BC29764	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46245	WGS84	BC29764	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.763	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29764_Mgig_Bot2012_COI	KEMA20	1	BC29764_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29764	Sample	BC29764	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA20	COI	45	BC29764	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA20	TCTTATTCGTTGGAGACTTTATAACCCT	150.89685
BC29765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46331	WGS84	BC29765	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29765_Cmae_Bot2011_COI	KEMA19	1	BC29765_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29765	Sample	BC29765	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA19	COI	45	BC29765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA19	ATGAACAGTCTATCCTCCTTTAG	150.90459
BC29765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46331	WGS84	BC29765	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29765_Msal_Bot2012_COI	KEMA19	1	BC29765_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29765	Sample	BC29765	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA19	COI	45	BC29765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA19	GYTAGTTCCRATGATGTTAGCTG	150.90459
BC29767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46402	WGS84	BC29767	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29767_Aamu_Bax2006_COI	KEMA17	1	BC29767_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29767	Sample	BC29767	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA17	COI	45	BC29767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA17	GCACAACCGGGATCTTTACTTC	150.90113
BC29767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46402	WGS84	BC29767	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29767_Pcan_Bot2011_IGS	KEMA17	1	BC29767_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29767	Sample	BC29767	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA17	IGS	45	BC29767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA17	CGTAATCCTCAGTACTGGCTA	150.90113
BC29767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46402	WGS84	BC29767	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29767_Sspa_Oph2007_COI	KEMA17	1	BC29767_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29767	Sample	BC29767	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA17	COI	45	BC29767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA17	GAAGGCCTTCCGATGATC	150.90113
BC29768	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46436	WGS84	BC29768	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.479	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29768_Mgig_Bot2012_COI	KEMA16	1	BC29768_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29768	Sample	BC29768	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA16	COI	45	BC29768	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA16	TCTTATTCGTTGGAGACTTTATAACCCT	150.90839
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46501	WGS84	BC29769	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29769_Cmae_Bot2011_COI	KEMA15	1	BC29769_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA15	COI	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	ATGAACAGTCTATCCTCCTTTAG	150.90702
BC29769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46501	WGS84	BC29769	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29769_Msal_Bot2012_COI	KEMA15	1	BC29769_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29769	Sample	BC29769	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA15	COI	45	BC29769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA15	GYTAGTTCCRATGATGTTAGCTG	150.90702
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46527	WGS84	BC29770	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29770_Aamu_Bax2006_COI	KEMA13	1	BC29770_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	GCACAACCGGGATCTTTACTTC	150.90138
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46527	WGS84	BC29770	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29770_Pcan_Bot2011_IGS	KEMA13	1	BC29770_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA13	IGS	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	CGTAATCCTCAGTACTGGCTA	150.90138
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46527	WGS84	BC29770	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29770_Sspa_Oph2007_COI	KEMA13	1	BC29770_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	GAAGGCCTTCCGATGATC	150.90138
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46527	WGS84	BC29770	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29770_Upin_Bot2011_COI	KEMA13	1	BC29770_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	CTTTAATTACAGCGTTTTTATTGTTGT	150.90138
BC29770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46527	WGS84	BC29770	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29770_Vgib_Wil2025_COI	KEMA13	1	BC29770_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29770	Sample	BC29770	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA13	COI	45	BC29770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA13	CCCCTCTTTACAAGTTCTTTAGGGT	150.90138
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46606	WGS84	BC29771	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29771_Asen_Wil2025_COI	KEMA12	1	BC29771_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	GTTGTTGTTACAACACATGCTCTTTTG	150.90836
BC29771	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46606	WGS84	BC29771	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.269	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29771_Mgig_Bot2012_COI	KEMA12	1	BC29771_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29771	Sample	BC29771	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA12	COI	45	BC29771	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA12	TCTTATTCGTTGGAGACTTTATAACCCT	150.90836
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46705	WGS84	BC29772	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29772_Cmae_Bot2011_COI	KEMA11	1	BC29772_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	ATGAACAGTCTATCCTCCTTTAG	150.90599
BC29772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46705	WGS84	BC29772	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29772_Msal_Bot2012_COI	KEMA11	1	BC29772_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29772	Sample	BC29772	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA11	COI	45	BC29772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA11	GYTAGTTCCRATGATGTTAGCTG	150.90599
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46537	WGS84	BC29774	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29774_Aamu_Bax2006_COI	KEMA14	1	BC29774_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	GCACAACCGGGATCTTTACTTC	150.90488
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46537	WGS84	BC29774	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29774_Pcan_Bot2011_IGS	KEMA14	1	BC29774_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA14	IGS	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	CGTAATCCTCAGTACTGGCTA	150.90488
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46537	WGS84	BC29774	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29774_Sspa_Oph2007_COI	KEMA14	1	BC29774_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	GAAGGCCTTCCGATGATC	150.90488
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46537	WGS84	BC29774	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29774_Upin_Bot2011_COI	KEMA14	1	BC29774_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	CTTTAATTACAGCGTTTTTATTGTTGT	150.90488
BC29774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46537	WGS84	BC29774	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29774_Vgib_Wil2025_COI	KEMA14	1	BC29774_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29774	Sample	BC29774	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA14	COI	45	BC29774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA14	CCCCTCTTTACAAGTTCTTTAGGGT	150.90488
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46673	WGS84	BC29775	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29775_Asen_Wil2025_COI	KEMA9	1	BC29775_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	GTTGTTGTTACAACACATGCTCTTTTG	150.90128
BC29775	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46673	WGS84	BC29775	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.772	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29775_Mgig_Bot2012_COI	KEMA9	1	BC29775_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29775	Sample	BC29775	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA9	COI	45	BC29775	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA9	TCTTATTCGTTGGAGACTTTATAACCCT	150.90128
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46841	WGS84	BC29776	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29776_Cmae_Bot2011_COI	KEMA8	1	BC29776_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	ATGAACAGTCTATCCTCCTTTAG	150.90758
BC29776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46841	WGS84	BC29776	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29776_Msal_Bot2012_COI	KEMA8	1	BC29776_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29776	Sample	BC29776	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA8	COI	45	BC29776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA8	GYTAGTTCCRATGATGTTAGCTG	150.90758
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4701	WGS84	BC29778	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29778_Aamu_Bax2006_COI	KEMA6	1	BC29778_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	GCACAACCGGGATCTTTACTTC	150.9063
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4701	WGS84	BC29778	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29778_Pcan_Bot2011_IGS	KEMA6	1	BC29778_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA6	IGS	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	CGTAATCCTCAGTACTGGCTA	150.9063
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4701	WGS84	BC29778	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29778_Sspa_Oph2007_COI	KEMA6	1	BC29778_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	GAAGGCCTTCCGATGATC	150.9063
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.4701	WGS84	BC29778	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29778_Upin_Bot2011_COI	KEMA6	1	BC29778_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	CTTTAATTACAGCGTTTTTATTGTTGT	150.9063
BC29778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.4701	WGS84	BC29778	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29778_Vgib_Wil2025_COI	KEMA6	1	BC29778_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29778	Sample	BC29778	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA6	COI	45	BC29778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA6	CCCCTCTTTACAAGTTCTTTAGGGT	150.9063
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.46879	WGS84	BC29779	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29779_Asen_Wil2025_COI	KEMA5	1	BC29779_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	GTTGTTGTTACAACACATGCTCTTTTG	150.91175
BC29779	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.46879	WGS84	BC29779	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.523	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29779_Mgig_Bot2012_COI	KEMA5	1	BC29779_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29779	Sample	BC29779	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA5	COI	45	BC29779	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA5	TCTTATTCGTTGGAGACTTTATAACCCT	150.91175
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47125	WGS84	BC29781	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29781_Aamu_Bax2006_COI	KEMA3	1	BC29781_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	GCACAACCGGGATCTTTACTTC	150.91025
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47125	WGS84	BC29781	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29781_Pcan_Bot2011_IGS	KEMA3	1	BC29781_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMA3	IGS	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	CGTAATCCTCAGTACTGGCTA	150.91025
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47125	WGS84	BC29781	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29781_Sspa_Oph2007_COI	KEMA3	1	BC29781_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	GAAGGCCTTCCGATGATC	150.91025
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47125	WGS84	BC29781	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29781_Upin_Bot2011_COI	KEMA3	1	BC29781_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Upin_Bot2011_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	CTTTAATTACAGCGTTTTTATTGTTGT	150.91025
BC29781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.47125	WGS84	BC29781	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC29781_Vgib_Wil2025_COI	KEMA3	1	BC29781_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29781	Sample	BC29781	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMA3	COI	45	BC29781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA3	CCCCTCTTTACAAGTTCTTTAGGGT	150.91025
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-34.47213	WGS84	BC29782	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC29782_Asen_Wil2025_COI	KEMA2	1	BC29782_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Absent	Sample	Asen_Wil2025_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	GTTGTTGTTACAACACATGCTCTTTTG	150.91224
BC29782	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47213	WGS84	BC29782	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	28.733	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29782_Mgig_Bot2012_COI	KEMA2	1	BC29782_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29782	Sample	BC29782	targeted	Present	Sample	Mgig_Bot2012_COI	KEMA2	COI	45	BC29782	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA2	TCTTATTCGTTGGAGACTTTATAACCCT	150.91224
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47312	WGS84	BC29783	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29783_Cmae_Bot2011_COI	KEMA1	1	BC29783_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	ATGAACAGTCTATCCTCCTTTAG	150.90939
BC29783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-34.47312	WGS84	BC29783	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Summer	3/04/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29783_Msal_Bot2012_COI	KEMA1	1	BC29783_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29783	Sample	BC29783	targeted	Absent	Sample	Msal_Bot2012_COI	KEMA1	COI	45	BC29783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMA1	GYTAGTTCCRATGATGTTAGCTG	150.90939
BC29785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.95907	WGS84	BC29785	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29785_Aamu_Bax2006_COI	BOTA42	1	BC29785_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29785	Sample	BC29785	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA42	COI	45	BC29785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA42	GCACAACCGGGATCTTTACTTC	151.1975
BC29785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.95907	WGS84	BC29785	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29785_Pcan_Bot2011_IGS	BOTA42	1	BC29785_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29785	Sample	BC29785	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA42	IGS	45	BC29785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA42	CGTAATCCTCAGTACTGGCTA	151.1975
BC29785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.95907	WGS84	BC29785	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29785_Sspa_Oph2007_COI	BOTA42	1	BC29785_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29785	Sample	BC29785	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA42	COI	45	BC29785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA42	GAAGGCCTTCCGATGATC	151.1975
BC29785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.95907	WGS84	BC29785	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29785_Upin_Bot2011_COI	BOTA42	1	BC29785_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29785	Sample	BC29785	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA42	COI	45	BC29785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA42	CTTTAATTACAGCGTTTTTATTGTTGT	151.1975
BC29786	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96074	WGS84	BC29786	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.766	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29786_Mgig_Bot2012_COI	BOTA41	1	BC29786_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29786	Sample	BC29786	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA41	COI	45	BC29786	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA41	TCTTATTCGTTGGAGACTTTATAACCCT	151.19651
BC29787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96424	WGS84	BC29787	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29787_Cmae_Bot2011_COI	BOTA39	1	BC29787_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29787	Sample	BC29787	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA39	COI	45	BC29787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA39	ATGAACAGTCTATCCTCCTTTAG	151.19718
BC29787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96424	WGS84	BC29787	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29787_Msal_Bot2012_COI	BOTA39	1	BC29787_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29787	Sample	BC29787	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA39	COI	45	BC29787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA39	GYTAGTTCCRATGATGTTAGCTG	151.19718
BC29789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96755	WGS84	BC29789	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29789_Aamu_Bax2006_COI	BOTA37	1	BC29789_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29789	Sample	BC29789	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA37	COI	45	BC29789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA37	GCACAACCGGGATCTTTACTTC	151.19821
BC29789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96755	WGS84	BC29789	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29789_Pcan_Bot2011_IGS	BOTA37	1	BC29789_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29789	Sample	BC29789	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA37	IGS	45	BC29789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA37	CGTAATCCTCAGTACTGGCTA	151.19821
BC29789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96755	WGS84	BC29789	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29789_Sspa_Oph2007_COI	BOTA37	1	BC29789_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29789	Sample	BC29789	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA37	COI	45	BC29789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA37	GAAGGCCTTCCGATGATC	151.19821
BC29789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96755	WGS84	BC29789	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29789_Upin_Bot2011_COI	BOTA37	1	BC29789_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29789	Sample	BC29789	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA37	COI	45	BC29789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA37	CTTTAATTACAGCGTTTTTATTGTTGT	151.19821
BC29790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96914	WGS84	BC29790	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29790_Cmae_Bot2011_COI	BOTA36	1	BC29790_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29790	Sample	BC29790	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA36	COI	45	BC29790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA36	ATGAACAGTCTATCCTCCTTTAG	151.19959
BC29790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96914	WGS84	BC29790	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29790_Msal_Bot2012_COI	BOTA36	1	BC29790_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29790	Sample	BC29790	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA36	COI	45	BC29790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA36	GYTAGTTCCRATGATGTTAGCTG	151.19959
BC29792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96878	WGS84	BC29792	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29792_Aamu_Bax2006_COI	BOTA34	1	BC29792_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29792	Sample	BC29792	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA34	COI	45	BC29792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA34	GCACAACCGGGATCTTTACTTC	151.21625
BC29792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96878	WGS84	BC29792	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29792_Pcan_Bot2011_IGS	BOTA34	1	BC29792_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29792	Sample	BC29792	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA34	IGS	45	BC29792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA34	CGTAATCCTCAGTACTGGCTA	151.21625
BC29792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96878	WGS84	BC29792	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29792_Sspa_Oph2007_COI	BOTA34	1	BC29792_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29792	Sample	BC29792	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA34	COI	45	BC29792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA34	GAAGGCCTTCCGATGATC	151.21625
BC29792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96878	WGS84	BC29792	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	28/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29792_Upin_Bot2011_COI	BOTA34	1	BC29792_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29792	Sample	BC29792	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA34	COI	45	BC29792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA34	CTTTAATTACAGCGTTTTTATTGTTGT	151.21625
BC29793	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.96896	WGS84	BC29793	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.898	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29793_Mgig_Bot2012_COI	BOTA33	1	BC29793_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29793	Sample	BC29793	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA33	COI	45	BC29793	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA33	TCTTATTCGTTGGAGACTTTATAACCCT	151.214
BC29794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97029	WGS84	BC29794	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29794_Cmae_Bot2011_COI	BOTA32	1	BC29794_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29794	Sample	BC29794	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA32	COI	45	BC29794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA32	ATGAACAGTCTATCCTCCTTTAG	151.20081
BC29794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97029	WGS84	BC29794	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29794_Msal_Bot2012_COI	BOTA32	1	BC29794_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29794	Sample	BC29794	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA32	COI	45	BC29794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA32	GYTAGTTCCRATGATGTTAGCTG	151.20081
BC29796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97016	WGS84	BC29796	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29796_Aamu_Bax2006_COI	BOTA30	1	BC29796_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29796	Sample	BC29796	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA30	COI	45	BC29796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA30	GCACAACCGGGATCTTTACTTC	151.21513
BC29796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97016	WGS84	BC29796	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29796_Pcan_Bot2011_IGS	BOTA30	1	BC29796_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29796	Sample	BC29796	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA30	IGS	45	BC29796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA30	CGTAATCCTCAGTACTGGCTA	151.21513
BC29796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97016	WGS84	BC29796	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29796_Sspa_Oph2007_COI	BOTA30	1	BC29796_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29796	Sample	BC29796	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA30	COI	45	BC29796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA30	GAAGGCCTTCCGATGATC	151.21513
BC29796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97016	WGS84	BC29796	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29796_Upin_Bot2011_COI	BOTA30	1	BC29796_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29796	Sample	BC29796	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA30	COI	45	BC29796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA30	CTTTAATTACAGCGTTTTTATTGTTGT	151.21513
BC29797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97063	WGS84	BC29797	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.681	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29797_Mgig_Bot2012_COI	BOTA29	1	BC29797_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29797	Sample	BC29797	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA29	COI	45	BC29797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA29	TCTTATTCGTTGGAGACTTTATAACCCT	151.21326
BC29798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97089	WGS84	BC29798	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29798_Cmae_Bot2011_COI	BOTA28	1	BC29798_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29798	Sample	BC29798	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA28	COI	45	BC29798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA28	ATGAACAGTCTATCCTCCTTTAG	151.21098
BC29798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97089	WGS84	BC29798	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29798_Msal_Bot2012_COI	BOTA28	1	BC29798_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29798	Sample	BC29798	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA28	COI	45	BC29798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA28	GYTAGTTCCRATGATGTTAGCTG	151.21098
BC29800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97167	WGS84	BC29800	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29800_Aamu_Bax2006_COI	BOTA26	1	BC29800_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29800	Sample	BC29800	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA26	COI	45	BC29800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA26	GCACAACCGGGATCTTTACTTC	151.20769
BC29800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97167	WGS84	BC29800	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29800_Pcan_Bot2011_IGS	BOTA26	1	BC29800_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29800	Sample	BC29800	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA26	IGS	45	BC29800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA26	CGTAATCCTCAGTACTGGCTA	151.20769
BC29800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97167	WGS84	BC29800	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.625	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29800_Sspa_Oph2007_COI	BOTA26	1	BC29800_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29800	Sample	BC29800	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA26	COI	45	BC29800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA26	GAAGGCCTTCCGATGATC	151.20769
BC29800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97167	WGS84	BC29800	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29800_Upin_Bot2011_COI	BOTA26	1	BC29800_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29800	Sample	BC29800	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA26	COI	45	BC29800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA26	CTTTAATTACAGCGTTTTTATTGTTGT	151.20769
BC29801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97164	WGS84	BC29801	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.417	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29801_Mgig_Bot2012_COI	BOTA25	1	BC29801_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29801	Sample	BC29801	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA25	COI	45	BC29801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA25	TCTTATTCGTTGGAGACTTTATAACCCT	151.2054
BC29802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97198	WGS84	BC29802	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29802_Cmae_Bot2011_COI	BOTA24	1	BC29802_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29802	Sample	BC29802	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA24	COI	45	BC29802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA24	ATGAACAGTCTATCCTCCTTTAG	151.2028
BC29802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97198	WGS84	BC29802	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29802_Msal_Bot2012_COI	BOTA24	1	BC29802_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29802	Sample	BC29802	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA24	COI	45	BC29802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA24	GYTAGTTCCRATGATGTTAGCTG	151.2028
BC29804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9725	WGS84	BC29804	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29804_Aamu_Bax2006_COI	BOTA22	1	BC29804_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29804	Sample	BC29804	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA22	COI	45	BC29804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA22	GCACAACCGGGATCTTTACTTC	151.21045
BC29804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9725	WGS84	BC29804	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29804_Pcan_Bot2011_IGS	BOTA22	1	BC29804_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29804	Sample	BC29804	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA22	IGS	45	BC29804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA22	CGTAATCCTCAGTACTGGCTA	151.21045
BC29804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9725	WGS84	BC29804	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.153	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29804_Sspa_Oph2007_COI	BOTA22	1	BC29804_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29804	Sample	BC29804	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA22	COI	45	BC29804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA22	GAAGGCCTTCCGATGATC	151.21045
BC29804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9725	WGS84	BC29804	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29804_Upin_Bot2011_COI	BOTA22	1	BC29804_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29804	Sample	BC29804	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA22	COI	45	BC29804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA22	CTTTAATTACAGCGTTTTTATTGTTGT	151.21045
BC29805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97277	WGS84	BC29805	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.236	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29805_Mgig_Bot2012_COI	BOTA21	1	BC29805_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29805	Sample	BC29805	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA21	COI	45	BC29805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA21	TCTTATTCGTTGGAGACTTTATAACCCT	151.20822
BC29806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97333	WGS84	BC29806	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29806_Cmae_Bot2011_COI	BOTA20	1	BC29806_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29806	Sample	BC29806	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA20	COI	45	BC29806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA20	ATGAACAGTCTATCCTCCTTTAG	151.20691
BC29806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97333	WGS84	BC29806	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29806_Msal_Bot2012_COI	BOTA20	1	BC29806_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29806	Sample	BC29806	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA20	COI	45	BC29806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA20	GYTAGTTCCRATGATGTTAGCTG	151.20691
BC29808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97356	WGS84	BC29808	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29808_Aamu_Bax2006_COI	BOTA18	1	BC29808_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29808	Sample	BC29808	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA18	COI	45	BC29808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA18	GCACAACCGGGATCTTTACTTC	151.2016
BC29808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97356	WGS84	BC29808	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29808_Pcan_Bot2011_IGS	BOTA18	1	BC29808_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29808	Sample	BC29808	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA18	IGS	45	BC29808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA18	CGTAATCCTCAGTACTGGCTA	151.2016
BC29808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97356	WGS84	BC29808	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29808_Sspa_Oph2007_COI	BOTA18	1	BC29808_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29808	Sample	BC29808	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA18	COI	45	BC29808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA18	GAAGGCCTTCCGATGATC	151.2016
BC29808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97356	WGS84	BC29808	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29808_Upin_Bot2011_COI	BOTA18	1	BC29808_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29808	Sample	BC29808	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA18	COI	45	BC29808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA18	CTTTAATTACAGCGTTTTTATTGTTGT	151.2016
BC29809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.9739	WGS84	BC29809	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.834	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29809_Mgig_Bot2012_COI	BOTA17	1	BC29809_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29809	Sample	BC29809	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA17	COI	45	BC29809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA17	TCTTATTCGTTGGAGACTTTATAACCCT	151.20928
BC29811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97542	WGS84	BC29811	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29811_Aamu_Bax2006_COI	BOTA15	1	BC29811_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29811	Sample	BC29811	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA15	COI	45	BC29811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA15	GCACAACCGGGATCTTTACTTC	151.20835
BC29811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97542	WGS84	BC29811	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29811_Pcan_Bot2011_IGS	BOTA15	1	BC29811_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29811	Sample	BC29811	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA15	IGS	45	BC29811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA15	CGTAATCCTCAGTACTGGCTA	151.20835
BC29811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97542	WGS84	BC29811	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29811_Sspa_Oph2007_COI	BOTA15	1	BC29811_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29811	Sample	BC29811	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA15	COI	45	BC29811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA15	GAAGGCCTTCCGATGATC	151.20835
BC29811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97542	WGS84	BC29811	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29811_Upin_Bot2011_COI	BOTA15	1	BC29811_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29811	Sample	BC29811	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA15	COI	45	BC29811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA15	CTTTAATTACAGCGTTTTTATTGTTGT	151.20835
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29812_Cjap_Sim2018_COI	BOTA14	1	BC29812_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29812_Esin_And2018_Cyb	BOTA14	1	BC29812_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA14	Cytb	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	ACCCCTCCTCATATCCAACCA	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.111	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29812_Mgig_Bot2012_COI	BOTA14	1	BC29812_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	TCTTATTCGTTGGAGACTTTATAACCCT	151.20754
BC29812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97677	WGS84	BC29812	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29812_Mjap_Gib2022_COI	BOTA14	1	BC29812_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29812	Sample	BC29812	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA14	COI	45	BC29812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA14	AATTTAATTGTGACTAGGCATGGTCTG	151.20754
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29813_Cmae_Bot2011_COI	BOTA13	1	BC29813_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	ATGAACAGTCTATCCTCCTTTAG	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29813_Hsan_Wil2021_COI	BOTA13	1	BC29813_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	CCTGGGCCGGTATAGTAGGT	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29813_Mros_Gib2022_COI	BOTA13	1	BC29813_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29813_Msal_Bot2012_COI	BOTA13	1	BC29813_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	GYTAGTTCCRATGATGTTAGCTG	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29813_Pvir_Dia2013_COI	BOTA13	1	BC29813_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	CTTAGTGGCATTAATTCGDAATCC	151.20882
BC29813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.97772	WGS84	BC29813	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29813_Rhar_Sim2018_COI	BOTA13	1	BC29813_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29813	Sample	BC29813	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA13	COI	45	BC29813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA13	CTGCTGCTATTGCTCATG	151.20882
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29815_Aamu_Bax2006_COI	BOTA11	1	BC29815_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	GCACAACCGGGATCTTTACTTC	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29815_Pcan_Bot2011_IGS	BOTA11	1	BC29815_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA11	IGS	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	CGTAATCCTCAGTACTGGCTA	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29815_Pper_Dia2013_COI	BOTA11	1	BC29815_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	CTTAGTGGCATTAATTCGDAATCC	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29815_Sspa_Oph2007_COI	BOTA11	1	BC29815_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	GAAGGCCTTCCGATGATC	151.20777
BC29815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98016	WGS84	BC29815	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29815_Upin_Bot2011_COI	BOTA11	1	BC29815_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29815	Sample	BC29815	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA11	COI	45	BC29815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA11	CTTTAATTACAGCGTTTTTATTGTTGT	151.20777
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29816_Cjap_Sim2018_COI	BOTA10	1	BC29816_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29816_Esin_And2018_Cyb	BOTA10	1	BC29816_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA10	Cytb	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	ACCCCTCCTCATATCCAACCA	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	27.613	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29816_Mgig_Bot2012_COI	BOTA10	1	BC29816_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	TCTTATTCGTTGGAGACTTTATAACCCT	151.20684
BC29816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.98236	WGS84	BC29816	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29816_Mjap_Gib2022_COI	BOTA10	1	BC29816_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29816	Sample	BC29816	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA10	COI	45	BC29816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA10	AATTTAATTGTGACTAGGCATGGTCTG	151.20684
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29817_Cmae_Bot2011_COI	BOTA9	1	BC29817_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	ATGAACAGTCTATCCTCCTTTAG	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29817_Hsan_Wil2021_COI	BOTA9	1	BC29817_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	CCTGGGCCGGTATAGTAGGT	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29817_Mros_Gib2022_COI	BOTA9	1	BC29817_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29817_Msal_Bot2012_COI	BOTA9	1	BC29817_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	GYTAGTTCCRATGATGTTAGCTG	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29817_Pvir_Dia2013_COI	BOTA9	1	BC29817_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	CTTAGTGGCATTAATTCGDAATCC	151.21203
BC29817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99596	WGS84	BC29817	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29817_Rhar_Sim2018_COI	BOTA9	1	BC29817_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29817	Sample	BC29817	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA9	COI	45	BC29817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA9	CTGCTGCTATTGCTCATG	151.21203
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29819_Aamu_Bax2006_COI	BOTA7	1	BC29819_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	GCACAACCGGGATCTTTACTTC	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29819_Pcan_Bot2011_IGS	BOTA7	1	BC29819_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA7	IGS	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	CGTAATCCTCAGTACTGGCTA	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29819_Pper_Dia2013_COI	BOTA7	1	BC29819_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	CTTAGTGGCATTAATTCGDAATCC	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.49	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29819_Sspa_Oph2007_COI	BOTA7	1	BC29819_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	GAAGGCCTTCCGATGATC	151.21359
BC29819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99802	WGS84	BC29819	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29819_Upin_Bot2011_COI	BOTA7	1	BC29819_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29819	Sample	BC29819	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA7	COI	45	BC29819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA7	CTTTAATTACAGCGTTTTTATTGTTGT	151.21359
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29820_Cmae_Bot2011_COI	BOTA6	1	BC29820_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	ATGAACAGTCTATCCTCCTTTAG	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29820_Hsan_Wil2021_COI	BOTA6	1	BC29820_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	CCTGGGCCGGTATAGTAGGT	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29820_Mros_Gib2022_COI	BOTA6	1	BC29820_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29820_Msal_Bot2012_COI	BOTA6	1	BC29820_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	GYTAGTTCCRATGATGTTAGCTG	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29820_Pvir_Dia2013_COI	BOTA6	1	BC29820_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	CTTAGTGGCATTAATTCGDAATCC	151.20998
BC29820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99813	WGS84	BC29820	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	27/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29820_Rhar_Sim2018_COI	BOTA6	1	BC29820_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29820	Sample	BC29820	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA6	COI	45	BC29820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA6	CTGCTGCTATTGCTCATG	151.20998
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29822_Aamu_Bax2006_COI	BOTA4	1	BC29822_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	GCACAACCGGGATCTTTACTTC	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29822_Pcan_Bot2011_IGS	BOTA4	1	BC29822_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTA4	IGS	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	CGTAATCCTCAGTACTGGCTA	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC29822_Pper_Dia2013_COI	BOTA4	1	BC29822_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Pper_Dia2013_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	CTTAGTGGCATTAATTCGDAATCC	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.2	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29822_Sspa_Oph2007_COI	BOTA4	1	BC29822_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Present	Sample	Sspa_Oph2007_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	GAAGGCCTTCCGATGATC	151.21232
BC29822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-33.99986	WGS84	BC29822	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29822_Upin_Bot2011_COI	BOTA4	1	BC29822_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29822	Sample	BC29822	targeted	Absent	Sample	Upin_Bot2011_COI	BOTA4	COI	45	BC29822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA4	CTTTAATTACAGCGTTTTTATTGTTGT	151.21232
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC29823_Cjap_Sim2018_COI	BOTA3	1	BC29823_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Cjap_Sim2018_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC29823_Esin_And2018_Cyb	BOTA3	1	BC29823_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Esin_And2018_Cyb	BOTA3	Cytb	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	ACCCCTCCTCATATCCAACCA	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.032	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29823_Mgig_Bot2012_COI	BOTA3	1	BC29823_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Present	Sample	Mgig_Bot2012_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	TCTTATTCGTTGGAGACTTTATAACCCT	151.20712
BC29823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00016	WGS84	BC29823	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC29823_Mjap_Gib2022_COI	BOTA3	1	BC29823_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29823	Sample	BC29823	targeted	Absent	Sample	Mjap_Gib2022_COI	BOTA3	COI	45	BC29823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA3	AATTTAATTGTGACTAGGCATGGTCTG	151.20712
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29824_Cmae_Bot2011_COI	BOTA1	1	BC29824_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	ATGAACAGTCTATCCTCCTTTAG	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC29824_Hsan_Wil2021_COI	BOTA1	1	BC29824_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Hsan_Wil2021_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	CCTGGGCCGGTATAGTAGGT	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC29824_Mros_Gib2022_COI	BOTA1	1	BC29824_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Mros_Gib2022_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29824_Msal_Bot2012_COI	BOTA1	1	BC29824_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Msal_Bot2012_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	GYTAGTTCCRATGATGTTAGCTG	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC29824_Pvir_Dia2013_COI	BOTA1	1	BC29824_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Pvir_Dia2013_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	CTTAGTGGCATTAATTCGDAATCC	151.20991
BC29824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12160	-34.00295	WGS84	BC29824	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2018-19_Molecular_surveys-Botany_Summer	26/02/2019	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC29824_Rhar_Sim2018_COI	BOTA1	1	BC29824_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29824	Sample	BC29824	targeted	Absent	Sample	Rhar_Sim2018_COI	BOTA1	COI	45	BC29824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTA1	CTGCTGCTATTGCTCATG	151.20991
BC29826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66552	WGS84	BC29826	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29826_Aamu_Bax2006_COI	WEIA5	1	BC29826_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29826	Sample	BC29826	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA5	COI	45	BC29826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA5	GCACAACCGGGATCTTTACTTC	141.85005
BC29826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66552	WGS84	BC29826	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29826_Pcan_Bot2011_IGS	WEIA5	1	BC29826_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29826	Sample	BC29826	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA5	IGS	45	BC29826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA5	CGTAATCCTCAGTACTGGCTA	141.85005
BC29826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66552	WGS84	BC29826	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29826_Sspa_Oph2007_COI	WEIA5	1	BC29826_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29826	Sample	BC29826	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA5	COI	45	BC29826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA5	GAAGGCCTTCCGATGATC	141.85005
BC29826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66552	WGS84	BC29826	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29826_Upin_Bot2011_COI	WEIA5	1	BC29826_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29826	Sample	BC29826	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA5	COI	45	BC29826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA5	CTTTAATTACAGCGTTTTTATTGTTGT	141.85005
BC29827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67287	WGS84	BC29827	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29827_Mgig_Bot2012_COI	WEIA19	1	BC29827_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29827	Sample	BC29827	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA19	COI	45	BC29827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA19	TCTTATTCGTTGGAGACTTTATAACCCT	141.86564
BC29828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67224	WGS84	BC29828	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29828_Cmae_Bot2011_COI	WEIA18	1	BC29828_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29828	Sample	BC29828	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA18	COI	45	BC29828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA18	ATGAACAGTCTATCCTCCTTTAG	141.86659
BC29828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67224	WGS84	BC29828	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29828_Msal_Bot2012_COI	WEIA18	1	BC29828_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29828	Sample	BC29828	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA18	COI	45	BC29828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA18	GYTAGTTCCRATGATGTTAGCTG	141.86659
BC29830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67318	WGS84	BC29830	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29830_Mgig_Bot2012_COI	WEIA16	1	BC29830_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29830	Sample	BC29830	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA16	COI	45	BC29830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA16	TCTTATTCGTTGGAGACTTTATAACCCT	141.86865
BC29831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67395	WGS84	BC29831	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29831_Cmae_Bot2011_COI	WEIA14	1	BC29831_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29831	Sample	BC29831	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA14	COI	45	BC29831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA14	ATGAACAGTCTATCCTCCTTTAG	141.8665
BC29831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67395	WGS84	BC29831	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29831_Msal_Bot2012_COI	WEIA14	1	BC29831_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29831	Sample	BC29831	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA14	COI	45	BC29831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA14	GYTAGTTCCRATGATGTTAGCTG	141.8665
BC29833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67486	WGS84	BC29833	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29833_Aamu_Bax2006_COI	WEIA12	1	BC29833_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29833	Sample	BC29833	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA12	COI	45	BC29833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA12	GCACAACCGGGATCTTTACTTC	141.87001
BC29833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67486	WGS84	BC29833	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29833_Pcan_Bot2011_IGS	WEIA12	1	BC29833_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29833	Sample	BC29833	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA12	IGS	45	BC29833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA12	CGTAATCCTCAGTACTGGCTA	141.87001
BC29833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67486	WGS84	BC29833	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29833_Sspa_Oph2007_COI	WEIA12	1	BC29833_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29833	Sample	BC29833	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA12	COI	45	BC29833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA12	GAAGGCCTTCCGATGATC	141.87001
BC29833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67486	WGS84	BC29833	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29833_Upin_Bot2011_COI	WEIA12	1	BC29833_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29833	Sample	BC29833	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA12	COI	45	BC29833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA12	CTTTAATTACAGCGTTTTTATTGTTGT	141.87001
BC29834	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67501	WGS84	BC29834	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29834_Mgig_Bot2012_COI	WEIA11	1	BC29834_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29834	Sample	BC29834	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA11	COI	45	BC29834	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA11	TCTTATTCGTTGGAGACTTTATAACCCT	141.86849
BC29835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66819	WGS84	BC29835	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29835_Cmae_Bot2011_COI	WEIA9	1	BC29835_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29835	Sample	BC29835	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA9	COI	45	BC29835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA9	ATGAACAGTCTATCCTCCTTTAG	141.86086
BC29835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66819	WGS84	BC29835	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	19/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29835_Msal_Bot2012_COI	WEIA9	1	BC29835_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29835	Sample	BC29835	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA9	COI	45	BC29835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA9	GYTAGTTCCRATGATGTTAGCTG	141.86086
BC29837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67017	WGS84	BC29837	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29837_Aamu_Bax2006_COI	WEIA7	1	BC29837_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29837	Sample	BC29837	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA7	COI	45	BC29837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA7	GCACAACCGGGATCTTTACTTC	141.86209
BC29837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67017	WGS84	BC29837	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29837_Pcan_Bot2011_IGS	WEIA7	1	BC29837_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29837	Sample	BC29837	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA7	IGS	45	BC29837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA7	CGTAATCCTCAGTACTGGCTA	141.86209
BC29837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67017	WGS84	BC29837	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29837_Sspa_Oph2007_COI	WEIA7	1	BC29837_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29837	Sample	BC29837	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA7	COI	45	BC29837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA7	GAAGGCCTTCCGATGATC	141.86209
BC29837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.67017	WGS84	BC29837	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29837_Upin_Bot2011_COI	WEIA7	1	BC29837_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29837	Sample	BC29837	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA7	COI	45	BC29837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA7	CTTTAATTACAGCGTTTTTATTGTTGT	141.86209
BC29838	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66604	WGS84	BC29838	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29838_Mgig_Bot2012_COI	WEIA4	1	BC29838_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29838	Sample	BC29838	targeted	Absent	Sample	Mgig_Bot2012_COI	WEIA4	COI	45	BC29838	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA4	TCTTATTCGTTGGAGACTTTATAACCCT	141.84772
BC29839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66715	WGS84	BC29839	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29839_Cmae_Bot2011_COI	WEIA3	1	BC29839_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29839	Sample	BC29839	targeted	Absent	Sample	Cmae_Bot2011_COI	WEIA3	COI	45	BC29839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA3	ATGAACAGTCTATCCTCCTTTAG	141.84948
BC29839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.66715	WGS84	BC29839	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29839_Msal_Bot2012_COI	WEIA3	1	BC29839_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29839	Sample	BC29839	targeted	Absent	Sample	Msal_Bot2012_COI	WEIA3	COI	45	BC29839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA3	GYTAGTTCCRATGATGTTAGCTG	141.84948
BC29840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.6676	WGS84	BC29840	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29840_Aamu_Bax2006_COI	WEIA1	1	BC29840_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29840	Sample	BC29840	targeted	Absent	Sample	Aamu_Bax2006_COI	WEIA1	COI	45	BC29840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA1	GCACAACCGGGATCTTTACTTC	141.846
BC29840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.6676	WGS84	BC29840	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29840_Pcan_Bot2011_IGS	WEIA1	1	BC29840_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29840	Sample	BC29840	targeted	Absent	Sample	Pcan_Bot2011_IGS	WEIA1	IGS	45	BC29840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA1	CGTAATCCTCAGTACTGGCTA	141.846
BC29840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.6676	WGS84	BC29840	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29840_Sspa_Oph2007_COI	WEIA1	1	BC29840_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29840	Sample	BC29840	targeted	Absent	Sample	Sspa_Oph2007_COI	WEIA1	COI	45	BC29840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA1	GAAGGCCTTCCGATGATC	141.846
BC29840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.6676	WGS84	BC29840	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Weipa_Summer	18/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29840_Upin_Bot2011_COI	WEIA1	1	BC29840_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29840	Sample	BC29840	targeted	Absent	Sample	Upin_Bot2011_COI	WEIA1	COI	45	BC29840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	WEIA1	CTTTAATTACAGCGTTTTTATTGTTGT	141.846
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20676	WGS84	BC29846	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29846_Cmae_Bot2011_COI	GOVA15	1	BC29846_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	ATGAACAGTCTATCCTCCTTTAG	136.66753
BC29846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20676	WGS84	BC29846	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29846_Msal_Bot2012_COI	GOVA15	1	BC29846_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29846	Sample	BC29846	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA15	COI	45	BC29846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA15	GYTAGTTCCRATGATGTTAGCTG	136.66753
BC29848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20461	WGS84	BC29848	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29848_Aamu_Bax2006_COI	GOVA5	1	BC29848_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29848	Sample	BC29848	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA5	COI	45	BC29848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA5	GCACAACCGGGATCTTTACTTC	136.68266
BC29848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20461	WGS84	BC29848	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29848_Pcan_Bot2011_IGS	GOVA5	1	BC29848_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29848	Sample	BC29848	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA5	IGS	45	BC29848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA5	CGTAATCCTCAGTACTGGCTA	136.68266
BC29848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20461	WGS84	BC29848	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29848_Sspa_Oph2007_COI	GOVA5	1	BC29848_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29848	Sample	BC29848	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA5	COI	45	BC29848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA5	GAAGGCCTTCCGATGATC	136.68266
BC29848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20461	WGS84	BC29848	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29848_Upin_Bot2011_COI	GOVA5	1	BC29848_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29848	Sample	BC29848	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA5	COI	45	BC29848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA5	CTTTAATTACAGCGTTTTTATTGTTGT	136.68266
BC29849	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20293	WGS84	BC29849	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29849_Mgig_Bot2012_COI	GOVA22	1	BC29849_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29849	Sample	BC29849	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA22	COI	45	BC29849	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA22	TCTTATTCGTTGGAGACTTTATAACCCT	136.66973
BC29851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20509	WGS84	BC29851	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29851_Aamu_Bax2006_COI	GOVA19	1	BC29851_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29851	Sample	BC29851	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA19	COI	45	BC29851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA19	GCACAACCGGGATCTTTACTTC	136.66844
BC29851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20509	WGS84	BC29851	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29851_Pcan_Bot2011_IGS	GOVA19	1	BC29851_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29851	Sample	BC29851	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA19	IGS	45	BC29851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA19	CGTAATCCTCAGTACTGGCTA	136.66844
BC29851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20509	WGS84	BC29851	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29851_Sspa_Oph2007_COI	GOVA19	1	BC29851_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29851	Sample	BC29851	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA19	COI	45	BC29851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA19	GAAGGCCTTCCGATGATC	136.66844
BC29851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20509	WGS84	BC29851	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29851_Upin_Bot2011_COI	GOVA19	1	BC29851_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29851	Sample	BC29851	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA19	COI	45	BC29851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA19	CTTTAATTACAGCGTTTTTATTGTTGT	136.66844
BC29852	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20625	WGS84	BC29852	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29852_Mgig_Bot2012_COI	GOVA17	1	BC29852_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29852	Sample	BC29852	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA17	COI	45	BC29852	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA17	TCTTATTCGTTGGAGACTTTATAACCCT	136.6721
BC29853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20777	WGS84	BC29853	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29853_Cmae_Bot2011_COI	GOVA13	1	BC29853_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29853	Sample	BC29853	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA13	COI	45	BC29853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA13	ATGAACAGTCTATCCTCCTTTAG	136.66944
BC29853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20777	WGS84	BC29853	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	22/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29853_Msal_Bot2012_COI	GOVA13	1	BC29853_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29853	Sample	BC29853	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA13	COI	45	BC29853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA13	GYTAGTTCCRATGATGTTAGCTG	136.66944
BC29855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.21015	WGS84	BC29855	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29855_Aamu_Bax2006_COI	GOVA9	1	BC29855_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29855	Sample	BC29855	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA9	COI	45	BC29855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA9	GCACAACCGGGATCTTTACTTC	136.67167
BC29855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.21015	WGS84	BC29855	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29855_Pcan_Bot2011_IGS	GOVA9	1	BC29855_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29855	Sample	BC29855	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA9	IGS	45	BC29855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA9	CGTAATCCTCAGTACTGGCTA	136.67167
BC29855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.21015	WGS84	BC29855	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29855_Sspa_Oph2007_COI	GOVA9	1	BC29855_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29855	Sample	BC29855	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA9	COI	45	BC29855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA9	GAAGGCCTTCCGATGATC	136.67167
BC29855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.21015	WGS84	BC29855	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29855_Upin_Bot2011_COI	GOVA9	1	BC29855_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29855	Sample	BC29855	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA9	COI	45	BC29855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA9	CTTTAATTACAGCGTTTTTATTGTTGT	136.67167
BC29856	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.20147	WGS84	BC29856	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC29856_Mgig_Bot2012_COI	GOVA7	1	BC29856_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29856	Sample	BC29856	targeted	Absent	Sample	Mgig_Bot2012_COI	GOVA7	COI	45	BC29856	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA7	TCTTATTCGTTGGAGACTTTATAACCCT	136.68073
BC29857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.2028	WGS84	BC29857	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29857_Cmae_Bot2011_COI	GOVA6	1	BC29857_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29857	Sample	BC29857	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA6	COI	45	BC29857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA6	ATGAACAGTCTATCCTCCTTTAG	136.68155
BC29857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.2028	WGS84	BC29857	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29857_Msal_Bot2012_COI	GOVA6	1	BC29857_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29857	Sample	BC29857	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA6	COI	45	BC29857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA6	GYTAGTTCCRATGATGTTAGCTG	136.68155
BC29859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19561	WGS84	BC29859	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC29859_Aamu_Bax2006_COI	GOVA2	1	BC29859_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29859	Sample	BC29859	targeted	Absent	Sample	Aamu_Bax2006_COI	GOVA2	COI	45	BC29859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA2	GCACAACCGGGATCTTTACTTC	136.68673
BC29859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19561	WGS84	BC29859	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC29859_Pcan_Bot2011_IGS	GOVA2	1	BC29859_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29859	Sample	BC29859	targeted	Absent	Sample	Pcan_Bot2011_IGS	GOVA2	IGS	45	BC29859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA2	CGTAATCCTCAGTACTGGCTA	136.68673
BC29859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19561	WGS84	BC29859	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC29859_Sspa_Oph2007_COI	GOVA2	1	BC29859_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29859	Sample	BC29859	targeted	Absent	Sample	Sspa_Oph2007_COI	GOVA2	COI	45	BC29859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA2	GAAGGCCTTCCGATGATC	136.68673
BC29859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19561	WGS84	BC29859	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC29859_Upin_Bot2011_COI	GOVA2	1	BC29859_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29859	Sample	BC29859	targeted	Absent	Sample	Upin_Bot2011_COI	GOVA2	COI	45	BC29859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA2	CTTTAATTACAGCGTTTTTATTGTTGT	136.68673
BC29860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19556	WGS84	BC29860	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC29860_Cmae_Bot2011_COI	GOVA1	1	BC29860_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29860	Sample	BC29860	targeted	Absent	Sample	Cmae_Bot2011_COI	GOVA1	COI	45	BC29860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA1	ATGAACAGTCTATCCTCCTTTAG	136.68512
BC29860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12159	-12.19556	WGS84	BC29860	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Gove_Summer	21/02/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC29860_Msal_Bot2012_COI	GOVA1	1	BC29860_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC29860	Sample	BC29860	targeted	Absent	Sample	Msal_Bot2012_COI	GOVA1	COI	45	BC29860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GOVA1	GYTAGTTCCRATGATGTTAGCTG	136.68512
BC37387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99876	WGS84	BC37387	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37387_Mgig_Bot2012_COI	BOTB6	1	BC37387_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37387	Sample	BC37387	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB6	COI	45	BC37387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB6	TCTTATTCGTTGGAGACTTTATAACCCT	151.2101
BC37388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99816	WGS84	BC37388	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37388_Cmae_Bot2011_COI	BOTB7	1	BC37388_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37388	Sample	BC37388	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB7	COI	45	BC37388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB7	ATGAACAGTCTATCCTCCTTTAG	151.21204
BC37388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99816	WGS84	BC37388	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37388_Msal_Bot2012_COI	BOTB7	1	BC37388_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37388	Sample	BC37388	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB7	COI	45	BC37388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB7	GYTAGTTCCRATGATGTTAGCTG	151.21204
BC37390	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99718	WGS84	BC37390	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37390_Mgig_Bot2012_COI	BOTB9	1	BC37390_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37390	Sample	BC37390	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB9	COI	45	BC37390	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB9	TCTTATTCGTTGGAGACTTTATAACCCT	151.21115
BC37391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98273	WGS84	BC37391	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37391_Cmae_Bot2011_COI	BOTB10	1	BC37391_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37391	Sample	BC37391	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB10	COI	45	BC37391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB10	ATGAACAGTCTATCCTCCTTTAG	151.20824
BC37391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.98273	WGS84	BC37391	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37391_Msal_Bot2012_COI	BOTB10	1	BC37391_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37391	Sample	BC37391	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB10	COI	45	BC37391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB10	GYTAGTTCCRATGATGTTAGCTG	151.20824
BC37393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97923	WGS84	BC37393	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37393_Aamu_Bax2006_COI	BOTB12	1	BC37393_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37393	Sample	BC37393	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB12	COI	45	BC37393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB12	GCACAACCGGGATCTTTACTTC	151.20787
BC37393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97923	WGS84	BC37393	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37393_Pcan_Bot2011_IGS	BOTB12	1	BC37393_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37393	Sample	BC37393	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB12	IGS	45	BC37393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB12	CGTAATCCTCAGTACTGGCTA	151.20787
BC37393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97923	WGS84	BC37393	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37393_Sspa_Oph2007_COI	BOTB12	1	BC37393_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37393	Sample	BC37393	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB12	COI	45	BC37393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB12	GAAGGCCTTCCGATGATC	151.20787
BC37393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97923	WGS84	BC37393	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37393_Upin_Bot2011_COI	BOTB12	1	BC37393_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37393	Sample	BC37393	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB12	COI	45	BC37393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB12	CTTTAATTACAGCGTTTTTATTGTTGT	151.20787
BC37394	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97853	WGS84	BC37394	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37394_Mgig_Bot2012_COI	BOTB13	1	BC37394_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37394	Sample	BC37394	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB13	COI	45	BC37394	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB13	TCTTATTCGTTGGAGACTTTATAACCCT	151.20963
BC37395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9773	WGS84	BC37395	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37395_Cmae_Bot2011_COI	BOTB14	1	BC37395_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37395	Sample	BC37395	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB14	COI	45	BC37395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB14	ATGAACAGTCTATCCTCCTTTAG	151.20861
BC37395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9773	WGS84	BC37395	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37395_Msal_Bot2012_COI	BOTB14	1	BC37395_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37395	Sample	BC37395	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB14	COI	45	BC37395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB14	GYTAGTTCCRATGATGTTAGCTG	151.20861
BC37397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97578	WGS84	BC37397	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37397_Aamu_Bax2006_COI	BOTB16	1	BC37397_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37397	Sample	BC37397	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB16	COI	45	BC37397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB16	GCACAACCGGGATCTTTACTTC	151.2104
BC37397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97578	WGS84	BC37397	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37397_Pcan_Bot2011_IGS	BOTB16	1	BC37397_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37397	Sample	BC37397	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB16	IGS	45	BC37397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB16	CGTAATCCTCAGTACTGGCTA	151.2104
BC37397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97578	WGS84	BC37397	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	42.292	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37397_Sspa_Oph2007_COI	BOTB16	1	BC37397_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37397	Sample	BC37397	targeted	Present	Sample	Sspa_Oph2007_COI	BOTB16	COI	45	BC37397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB16	GAAGGCCTTCCGATGATC	151.2104
BC37397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97578	WGS84	BC37397	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37397_Upin_Bot2011_COI	BOTB16	1	BC37397_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37397	Sample	BC37397	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB16	COI	45	BC37397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB16	CTTTAATTACAGCGTTTTTATTGTTGT	151.2104
BC37398	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97457	WGS84	BC37398	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37398_Mgig_Bot2012_COI	BOTB17	1	BC37398_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37398	Sample	BC37398	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB17	COI	45	BC37398	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB17	TCTTATTCGTTGGAGACTTTATAACCCT	151.2098
BC37399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97298	WGS84	BC37399	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37399_Cmae_Bot2011_COI	BOTB18	1	BC37399_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37399	Sample	BC37399	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB18	COI	45	BC37399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB18	ATGAACAGTCTATCCTCCTTTAG	151.20234
BC37399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97298	WGS84	BC37399	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37399_Msal_Bot2012_COI	BOTB18	1	BC37399_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37399	Sample	BC37399	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB18	COI	45	BC37399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB18	GYTAGTTCCRATGATGTTAGCTG	151.20234
BC37401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97244	WGS84	BC37401	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37401_Aamu_Bax2006_COI	BOTB20	1	BC37401_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37401	Sample	BC37401	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB20	COI	45	BC37401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB20	GCACAACCGGGATCTTTACTTC	151.20641
BC37401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97244	WGS84	BC37401	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37401_Pcan_Bot2011_IGS	BOTB20	1	BC37401_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37401	Sample	BC37401	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB20	IGS	45	BC37401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB20	CGTAATCCTCAGTACTGGCTA	151.20641
BC37401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97244	WGS84	BC37401	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.231	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37401_Sspa_Oph2007_COI	BOTB20	1	BC37401_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37401	Sample	BC37401	targeted	Present	Sample	Sspa_Oph2007_COI	BOTB20	COI	45	BC37401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB20	GAAGGCCTTCCGATGATC	151.20641
BC37401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97244	WGS84	BC37401	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37401_Upin_Bot2011_COI	BOTB20	1	BC37401_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37401	Sample	BC37401	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB20	COI	45	BC37401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB20	CTTTAATTACAGCGTTTTTATTGTTGT	151.20641
BC37402	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97291	WGS84	BC37402	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37402_Mgig_Bot2012_COI	BOTB21	1	BC37402_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37402	Sample	BC37402	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB21	COI	45	BC37402	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB21	TCTTATTCGTTGGAGACTTTATAACCCT	151.20878
BC37403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97278	WGS84	BC37403	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37403_Cmae_Bot2011_COI	BOTB22	1	BC37403_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37403	Sample	BC37403	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB22	COI	45	BC37403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB22	ATGAACAGTCTATCCTCCTTTAG	151.21048
BC37403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97278	WGS84	BC37403	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37403_Msal_Bot2012_COI	BOTB22	1	BC37403_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37403	Sample	BC37403	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB22	COI	45	BC37403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB22	GYTAGTTCCRATGATGTTAGCTG	151.21048
BC37405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97152	WGS84	BC37405	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37405_Aamu_Bax2006_COI	BOTB24	1	BC37405_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37405	Sample	BC37405	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB24	COI	45	BC37405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB24	GCACAACCGGGATCTTTACTTC	151.20333
BC37405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97152	WGS84	BC37405	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37405_Pcan_Bot2011_IGS	BOTB24	1	BC37405_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37405	Sample	BC37405	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB24	IGS	45	BC37405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB24	CGTAATCCTCAGTACTGGCTA	151.20333
BC37405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97152	WGS84	BC37405	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	40.037	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37405_Sspa_Oph2007_COI	BOTB24	1	BC37405_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37405	Sample	BC37405	targeted	Present	Sample	Sspa_Oph2007_COI	BOTB24	COI	45	BC37405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB24	GAAGGCCTTCCGATGATC	151.20333
BC37405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97152	WGS84	BC37405	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37405_Upin_Bot2011_COI	BOTB24	1	BC37405_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37405	Sample	BC37405	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB24	COI	45	BC37405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB24	CTTTAATTACAGCGTTTTTATTGTTGT	151.20333
BC37406	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9714	WGS84	BC37406	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.659	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37406_Mgig_Bot2012_COI	BOTB25	1	BC37406_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37406	Sample	BC37406	targeted	Present	Sample	Mgig_Bot2012_COI	BOTB25	COI	45	BC37406	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB25	TCTTATTCGTTGGAGACTTTATAACCCT	151.20539
BC37407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97093	WGS84	BC37407	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37407_Cmae_Bot2011_COI	BOTB26	1	BC37407_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37407	Sample	BC37407	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB26	COI	45	BC37407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB26	ATGAACAGTCTATCCTCCTTTAG	151.20754
BC37407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97093	WGS84	BC37407	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37407_Msal_Bot2012_COI	BOTB26	1	BC37407_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37407	Sample	BC37407	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB26	COI	45	BC37407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB26	GYTAGTTCCRATGATGTTAGCTG	151.20754
BC37409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97049	WGS84	BC37409	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37409_Aamu_Bax2006_COI	BOTB28	1	BC37409_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37409	Sample	BC37409	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB28	COI	45	BC37409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB28	GCACAACCGGGATCTTTACTTC	151.21199
BC37409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97049	WGS84	BC37409	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37409_Pcan_Bot2011_IGS	BOTB28	1	BC37409_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37409	Sample	BC37409	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB28	IGS	45	BC37409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB28	CGTAATCCTCAGTACTGGCTA	151.21199
BC37409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97049	WGS84	BC37409	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37409_Sspa_Oph2007_COI	BOTB28	1	BC37409_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37409	Sample	BC37409	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB28	COI	45	BC37409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB28	GAAGGCCTTCCGATGATC	151.21199
BC37409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97049	WGS84	BC37409	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37409_Upin_Bot2011_COI	BOTB28	1	BC37409_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37409	Sample	BC37409	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB28	COI	45	BC37409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB28	CTTTAATTACAGCGTTTTTATTGTTGT	151.21199
BC37410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97018	WGS84	BC37410	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37410_Cmae_Bot2011_COI	BOTB29	1	BC37410_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37410	Sample	BC37410	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB29	COI	45	BC37410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB29	ATGAACAGTCTATCCTCCTTTAG	151.21416
BC37410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97018	WGS84	BC37410	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37410_Msal_Bot2012_COI	BOTB29	1	BC37410_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37410	Sample	BC37410	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB29	COI	45	BC37410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB29	GYTAGTTCCRATGATGTTAGCTG	151.21416
BC37412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97019	WGS84	BC37412	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37412_Aamu_Bax2006_COI	BOTB31	1	BC37412_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37412	Sample	BC37412	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB31	COI	45	BC37412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB31	GCACAACCGGGATCTTTACTTC	151.19852
BC37412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97019	WGS84	BC37412	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37412_Pcan_Bot2011_IGS	BOTB31	1	BC37412_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37412	Sample	BC37412	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB31	IGS	45	BC37412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB31	CGTAATCCTCAGTACTGGCTA	151.19852
BC37412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97019	WGS84	BC37412	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37412_Sspa_Oph2007_COI	BOTB31	1	BC37412_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37412	Sample	BC37412	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB31	COI	45	BC37412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB31	GAAGGCCTTCCGATGATC	151.19852
BC37412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97019	WGS84	BC37412	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37412_Upin_Bot2011_COI	BOTB31	1	BC37412_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37412	Sample	BC37412	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB31	COI	45	BC37412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB31	CTTTAATTACAGCGTTTTTATTGTTGT	151.19852
BC37413	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.97061	WGS84	BC37413	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37413_Mgig_Bot2012_COI	BOTB32	1	BC37413_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37413	Sample	BC37413	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB32	COI	45	BC37413	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB32	TCTTATTCGTTGGAGACTTTATAACCCT	151.20139
BC37414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9686	WGS84	BC37414	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37414_Cmae_Bot2011_COI	BOTB33	1	BC37414_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37414	Sample	BC37414	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB33	COI	45	BC37414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB33	ATGAACAGTCTATCCTCCTTTAG	151.21507
BC37414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9686	WGS84	BC37414	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37414_Msal_Bot2012_COI	BOTB33	1	BC37414_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37414	Sample	BC37414	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB33	COI	45	BC37414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB33	GYTAGTTCCRATGATGTTAGCTG	151.21507
BC37416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96834	WGS84	BC37416	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37416_Aamu_Bax2006_COI	BOTB35	1	BC37416_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37416	Sample	BC37416	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB35	COI	45	BC37416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB35	GCACAACCGGGATCTTTACTTC	151.21836
BC37416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96834	WGS84	BC37416	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37416_Pcan_Bot2011_IGS	BOTB35	1	BC37416_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37416	Sample	BC37416	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB35	IGS	45	BC37416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB35	CGTAATCCTCAGTACTGGCTA	151.21836
BC37416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96834	WGS84	BC37416	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37416_Sspa_Oph2007_COI	BOTB35	1	BC37416_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37416	Sample	BC37416	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB35	COI	45	BC37416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB35	GAAGGCCTTCCGATGATC	151.21836
BC37416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96834	WGS84	BC37416	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37416_Upin_Bot2011_COI	BOTB35	1	BC37416_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37416	Sample	BC37416	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB35	COI	45	BC37416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB35	CTTTAATTACAGCGTTTTTATTGTTGT	151.21836
BC37417	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.9683	WGS84	BC37417	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37417_Mgig_Bot2012_COI	BOTB36	1	BC37417_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37417	Sample	BC37417	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB36	COI	45	BC37417	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB36	TCTTATTCGTTGGAGACTTTATAACCCT	151.19945
BC37418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96688	WGS84	BC37418	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37418_Cmae_Bot2011_COI	BOTB37	1	BC37418_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37418	Sample	BC37418	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB37	COI	45	BC37418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB37	ATGAACAGTCTATCCTCCTTTAG	151.19813
BC37418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96688	WGS84	BC37418	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37418_Msal_Bot2012_COI	BOTB37	1	BC37418_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37418	Sample	BC37418	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB37	COI	45	BC37418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB37	GYTAGTTCCRATGATGTTAGCTG	151.19813
BC37420	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96368	WGS84	BC37420	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37420_Mgig_Bot2012_COI	BOTB39	1	BC37420_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37420	Sample	BC37420	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB39	COI	45	BC37420	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB39	TCTTATTCGTTGGAGACTTTATAACCCT	151.19844
BC37421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.95928	WGS84	BC37421	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37421_Cmae_Bot2011_COI	BOTB42	1	BC37421_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37421	Sample	BC37421	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB42	COI	45	BC37421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB42	ATGAACAGTCTATCCTCCTTTAG	151.19883
BC37421	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.95928	WGS84	BC37421	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37421_Msal_Bot2012_COI	BOTB42	1	BC37421_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37421	Sample	BC37421	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB42	COI	45	BC37421	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB42	GYTAGTTCCRATGATGTTAGCTG	151.19883
BC37424	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.96267	WGS84	BC37424	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	21/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37424_Mgig_Bot2012_COI	BOTB40	1	BC37424_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37424	Sample	BC37424	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB40	COI	45	BC37424	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB40	TCTTATTCGTTGGAGACTTTATAACCCT	151.19922
BC37427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88148	WGS84	BC37427	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37427_Aamu_Bax2006_COI	NEWB45	1	BC37427_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37427	Sample	BC37427	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB45	COI	45	BC37427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB45	GCACAACCGGGATCTTTACTTC	151.74943
BC37427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88148	WGS84	BC37427	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37427_Pcan_Bot2011_IGS	NEWB45	1	BC37427_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37427	Sample	BC37427	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB45	IGS	45	BC37427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB45	CGTAATCCTCAGTACTGGCTA	151.74943
BC37427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88148	WGS84	BC37427	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37427_Sspa_Oph2007_COI	NEWB45	1	BC37427_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37427	Sample	BC37427	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB45	COI	45	BC37427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB45	GAAGGCCTTCCGATGATC	151.74943
BC37427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88148	WGS84	BC37427	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37427_Upin_Bot2011_COI	NEWB45	1	BC37427_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37427	Sample	BC37427	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB45	COI	45	BC37427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB45	CTTTAATTACAGCGTTTTTATTGTTGT	151.74943
BC37428	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88286	WGS84	BC37428	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.869	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37428_Mgig_Bot2012_COI	NEWB44	1	BC37428_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37428	Sample	BC37428	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB44	COI	45	BC37428	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB44	TCTTATTCGTTGGAGACTTTATAACCCT	151.75313
BC37429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88427	WGS84	BC37429	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37429_Cmae_Bot2011_COI	NEWB43	1	BC37429_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37429	Sample	BC37429	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB43	COI	45	BC37429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB43	ATGAACAGTCTATCCTCCTTTAG	151.75678
BC37429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88427	WGS84	BC37429	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37429_Msal_Bot2012_COI	NEWB43	1	BC37429_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37429	Sample	BC37429	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB43	COI	45	BC37429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB43	GYTAGTTCCRATGATGTTAGCTG	151.75678
BC37430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88439	WGS84	BC37430	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37430_Aamu_Bax2006_COI	NEWB42	1	BC37430_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37430	Sample	BC37430	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB42	COI	45	BC37430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB42	GCACAACCGGGATCTTTACTTC	151.75453
BC37430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88439	WGS84	BC37430	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37430_Pcan_Bot2011_IGS	NEWB42	1	BC37430_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37430	Sample	BC37430	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB42	IGS	45	BC37430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB42	CGTAATCCTCAGTACTGGCTA	151.75453
BC37430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88439	WGS84	BC37430	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37430_Sspa_Oph2007_COI	NEWB42	1	BC37430_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37430	Sample	BC37430	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB42	COI	45	BC37430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB42	GAAGGCCTTCCGATGATC	151.75453
BC37430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88439	WGS84	BC37430	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37430_Upin_Bot2011_COI	NEWB42	1	BC37430_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37430	Sample	BC37430	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB42	COI	45	BC37430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB42	CTTTAATTACAGCGTTTTTATTGTTGT	151.75453
BC37431	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88566	WGS84	BC37431	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.508	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37431_Mgig_Bot2012_COI	NEWB41	1	BC37431_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37431	Sample	BC37431	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB41	COI	45	BC37431	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB41	TCTTATTCGTTGGAGACTTTATAACCCT	151.75829
BC37432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88519	WGS84	BC37432	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37432_Cmae_Bot2011_COI	NEWB40	1	BC37432_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37432	Sample	BC37432	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB40	COI	45	BC37432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB40	ATGAACAGTCTATCCTCCTTTAG	151.75593
BC37432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88519	WGS84	BC37432	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37432_Msal_Bot2012_COI	NEWB40	1	BC37432_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37432	Sample	BC37432	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB40	COI	45	BC37432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB40	GYTAGTTCCRATGATGTTAGCTG	151.75593
BC37434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8867	WGS84	BC37434	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37434_Aamu_Bax2006_COI	NEWB38	1	BC37434_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37434	Sample	BC37434	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB38	COI	45	BC37434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB38	GCACAACCGGGATCTTTACTTC	151.75959
BC37434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8867	WGS84	BC37434	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37434_Pcan_Bot2011_IGS	NEWB38	1	BC37434_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37434	Sample	BC37434	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB38	IGS	45	BC37434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB38	CGTAATCCTCAGTACTGGCTA	151.75959
BC37434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8867	WGS84	BC37434	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37434_Sspa_Oph2007_COI	NEWB38	1	BC37434_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37434	Sample	BC37434	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB38	COI	45	BC37434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB38	GAAGGCCTTCCGATGATC	151.75959
BC37434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.8867	WGS84	BC37434	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37434_Upin_Bot2011_COI	NEWB38	1	BC37434_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37434	Sample	BC37434	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB38	COI	45	BC37434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB38	CTTTAATTACAGCGTTTTTATTGTTGT	151.75959
BC37435	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88803	WGS84	BC37435	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37435_Mgig_Bot2012_COI	NEWB37	1	BC37435_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37435	Sample	BC37435	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB37	COI	45	BC37435	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB37	TCTTATTCGTTGGAGACTTTATAACCCT	151.76579
BC37436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88799	WGS84	BC37436	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37436_Cmae_Bot2011_COI	NEWB36	1	BC37436_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37436	Sample	BC37436	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB36	COI	45	BC37436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB36	ATGAACAGTCTATCCTCCTTTAG	151.76335
BC37436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88799	WGS84	BC37436	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37436_Msal_Bot2012_COI	NEWB36	1	BC37436_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37436	Sample	BC37436	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB36	COI	45	BC37436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB36	GYTAGTTCCRATGATGTTAGCTG	151.76335
BC37438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88925	WGS84	BC37438	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37438_Aamu_Bax2006_COI	NEWB34	1	BC37438_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37438	Sample	BC37438	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB34	COI	45	BC37438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB34	GCACAACCGGGATCTTTACTTC	151.76953
BC37438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88925	WGS84	BC37438	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37438_Pcan_Bot2011_IGS	NEWB34	1	BC37438_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37438	Sample	BC37438	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB34	IGS	45	BC37438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB34	CGTAATCCTCAGTACTGGCTA	151.76953
BC37438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88925	WGS84	BC37438	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37438_Sspa_Oph2007_COI	NEWB34	1	BC37438_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37438	Sample	BC37438	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB34	COI	45	BC37438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB34	GAAGGCCTTCCGATGATC	151.76953
BC37438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88925	WGS84	BC37438	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37438_Upin_Bot2011_COI	NEWB34	1	BC37438_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37438	Sample	BC37438	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB34	COI	45	BC37438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB34	CTTTAATTACAGCGTTTTTATTGTTGT	151.76953
BC37439	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.88954	WGS84	BC37439	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.259	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37439_Mgig_Bot2012_COI	NEWB33	1	BC37439_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37439	Sample	BC37439	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB33	COI	45	BC37439	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB33	TCTTATTCGTTGGAGACTTTATAACCCT	151.76725
BC37441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89028	WGS84	BC37441	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37441_Aamu_Bax2006_COI	NEWB31	1	BC37441_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37441	Sample	BC37441	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB31	COI	45	BC37441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB31	GCACAACCGGGATCTTTACTTC	151.77213
BC37441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89028	WGS84	BC37441	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37441_Pcan_Bot2011_IGS	NEWB31	1	BC37441_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37441	Sample	BC37441	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB31	IGS	45	BC37441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB31	CGTAATCCTCAGTACTGGCTA	151.77213
BC37441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89028	WGS84	BC37441	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37441_Sspa_Oph2007_COI	NEWB31	1	BC37441_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37441	Sample	BC37441	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB31	COI	45	BC37441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB31	GAAGGCCTTCCGATGATC	151.77213
BC37441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89028	WGS84	BC37441	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37441_Upin_Bot2011_COI	NEWB31	1	BC37441_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37441	Sample	BC37441	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB31	COI	45	BC37441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB31	CTTTAATTACAGCGTTTTTATTGTTGT	151.77213
BC37442	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89074	WGS84	BC37442	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.623	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37442_Mgig_Bot2012_COI	NEWB30	1	BC37442_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37442	Sample	BC37442	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB30	COI	45	BC37442	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB30	TCTTATTCGTTGGAGACTTTATAACCCT	151.77097
BC37443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89124	WGS84	BC37443	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37443_Cmae_Bot2011_COI	NEWB29	1	BC37443_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37443	Sample	BC37443	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB29	COI	45	BC37443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB29	ATGAACAGTCTATCCTCCTTTAG	151.76864
BC37443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89124	WGS84	BC37443	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37443_Msal_Bot2012_COI	NEWB29	1	BC37443_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37443	Sample	BC37443	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB29	COI	45	BC37443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB29	GYTAGTTCCRATGATGTTAGCTG	151.76864
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37445_Aamu_Bax2006_COI	NEWB27	1	BC37445_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	GCACAACCGGGATCTTTACTTC	151.77239
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37445_Pcan_Bot2011_IGS	NEWB27	1	BC37445_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB27	IGS	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	CGTAATCCTCAGTACTGGCTA	151.77239
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37445_Sspa_Oph2007_COI	NEWB27	1	BC37445_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	GAAGGCCTTCCGATGATC	151.77239
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37445_Upin_Bot2011_COI	NEWB27	1	BC37445_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	CTTTAATTACAGCGTTTTTATTGTTGT	151.77239
BC37445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89217	WGS84	BC37445	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37445_Vgib_Wil2025_COI	NEWB27	1	BC37445_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37445	Sample	BC37445	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB27	COI	45	BC37445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB27	CCCCTCTTTACAAGTTCTTTAGGGT	151.77239
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37446_Asen_Wil2025_COI	NEWB26	1	BC37446_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Absent	Sample	Asen_Wil2025_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	GTTGTTGTTACAACACATGCTCTTTTG	151.77011
BC37446	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89254	WGS84	BC37446	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.236	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37446_Mgig_Bot2012_COI	NEWB26	1	BC37446_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37446	Sample	BC37446	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB26	COI	45	BC37446	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB26	TCTTATTCGTTGGAGACTTTATAACCCT	151.77011
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37447_Cmae_Bot2011_COI	NEWB25	1	BC37447_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	ATGAACAGTCTATCCTCCTTTAG	151.76678
BC37447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89267	WGS84	BC37447	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37447_Msal_Bot2012_COI	NEWB25	1	BC37447_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37447	Sample	BC37447	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB25	COI	45	BC37447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB25	GYTAGTTCCRATGATGTTAGCTG	151.76678
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37449_Aamu_Bax2006_COI	NEWB23	1	BC37449_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	GCACAACCGGGATCTTTACTTC	151.76928
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37449_Pcan_Bot2011_IGS	NEWB23	1	BC37449_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB23	IGS	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	CGTAATCCTCAGTACTGGCTA	151.76928
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37449_Sspa_Oph2007_COI	NEWB23	1	BC37449_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	GAAGGCCTTCCGATGATC	151.76928
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37449_Upin_Bot2011_COI	NEWB23	1	BC37449_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	CTTTAATTACAGCGTTTTTATTGTTGT	151.76928
BC37449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89448	WGS84	BC37449	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37449_Vgib_Wil2025_COI	NEWB23	1	BC37449_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37449	Sample	BC37449	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB23	COI	45	BC37449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB23	CCCCTCTTTACAAGTTCTTTAGGGT	151.76928
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37450_Cmae_Bot2011_COI	NEWB22	1	BC37450_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	ATGAACAGTCTATCCTCCTTTAG	151.77257
BC37450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.89614	WGS84	BC37450	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37450_Msal_Bot2012_COI	NEWB22	1	BC37450_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37450	Sample	BC37450	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB22	COI	45	BC37450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB22	GYTAGTTCCRATGATGTTAGCTG	151.77257
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37452_Aamu_Bax2006_COI	NEWB20	1	BC37452_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	GCACAACCGGGATCTTTACTTC	151.77454
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37452_Pcan_Bot2011_IGS	NEWB20	1	BC37452_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB20	IGS	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	CGTAATCCTCAGTACTGGCTA	151.77454
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37452_Sspa_Oph2007_COI	NEWB20	1	BC37452_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	GAAGGCCTTCCGATGATC	151.77454
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37452_Upin_Bot2011_COI	NEWB20	1	BC37452_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	CTTTAATTACAGCGTTTTTATTGTTGT	151.77454
BC37452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91198	WGS84	BC37452	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37452_Vgib_Wil2025_COI	NEWB20	1	BC37452_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37452	Sample	BC37452	targeted	Absent	Sample	Vgib_Wil2025_COI	NEWB20	COI	45	BC37452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB20	CCCCTCTTTACAAGTTCTTTAGGGT	151.77454
BC37453	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91353	WGS84	BC37453	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37453_Mgig_Bot2012_COI	NEWB19	1	BC37453_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37453	Sample	BC37453	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB19	COI	45	BC37453	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB19	TCTTATTCGTTGGAGACTTTATAACCCT	151.77278
BC37454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91478	WGS84	BC37454	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37454_Cmae_Bot2011_COI	NEWB18	1	BC37454_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37454	Sample	BC37454	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB18	COI	45	BC37454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB18	ATGAACAGTCTATCCTCCTTTAG	151.77479
BC37454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91478	WGS84	BC37454	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37454_Msal_Bot2012_COI	NEWB18	1	BC37454_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37454	Sample	BC37454	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB18	COI	45	BC37454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB18	GYTAGTTCCRATGATGTTAGCTG	151.77479
BC37456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91743	WGS84	BC37456	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37456_Aamu_Bax2006_COI	NEWB16	1	BC37456_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37456	Sample	BC37456	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB16	COI	45	BC37456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB16	GCACAACCGGGATCTTTACTTC	151.77377
BC37456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91743	WGS84	BC37456	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37456_Pcan_Bot2011_IGS	NEWB16	1	BC37456_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37456	Sample	BC37456	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB16	IGS	45	BC37456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB16	CGTAATCCTCAGTACTGGCTA	151.77377
BC37456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91743	WGS84	BC37456	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37456_Sspa_Oph2007_COI	NEWB16	1	BC37456_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37456	Sample	BC37456	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB16	COI	45	BC37456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB16	GAAGGCCTTCCGATGATC	151.77377
BC37456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91743	WGS84	BC37456	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37456_Upin_Bot2011_COI	NEWB16	1	BC37456_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37456	Sample	BC37456	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB16	COI	45	BC37456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB16	CTTTAATTACAGCGTTTTTATTGTTGT	151.77377
BC37457	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91821	WGS84	BC37457	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37457_Mgig_Bot2012_COI	NEWB15	1	BC37457_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37457	Sample	BC37457	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB15	COI	45	BC37457	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB15	TCTTATTCGTTGGAGACTTTATAACCCT	151.77566
BC37458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91994	WGS84	BC37458	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37458_Cmae_Bot2011_COI	NEWB14	1	BC37458_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37458	Sample	BC37458	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB14	COI	45	BC37458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB14	ATGAACAGTCTATCCTCCTTTAG	151.77723
BC37458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91994	WGS84	BC37458	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	16/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37458_Msal_Bot2012_COI	NEWB14	1	BC37458_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37458	Sample	BC37458	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB14	COI	45	BC37458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB14	GYTAGTTCCRATGATGTTAGCTG	151.77723
BC37460	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90041	WGS84	BC37460	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.295	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37460_Mgig_Bot2012_COI	NEWB12	1	BC37460_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37460	Sample	BC37460	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB12	COI	45	BC37460	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB12	TCTTATTCGTTGGAGACTTTATAACCCT	151.77233
BC37461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90277	WGS84	BC37461	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37461_Cmae_Bot2011_COI	NEWB11	1	BC37461_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37461	Sample	BC37461	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB11	COI	45	BC37461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB11	ATGAACAGTCTATCCTCCTTTAG	151.77329
BC37461	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90277	WGS84	BC37461	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37461_Msal_Bot2012_COI	NEWB11	1	BC37461_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37461	Sample	BC37461	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB11	COI	45	BC37461	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB11	GYTAGTTCCRATGATGTTAGCTG	151.77329
BC37463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90467	WGS84	BC37463	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37463_Aamu_Bax2006_COI	NEWB9	1	BC37463_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37463	Sample	BC37463	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB9	COI	45	BC37463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB9	GCACAACCGGGATCTTTACTTC	151.77235
BC37463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90467	WGS84	BC37463	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37463_Pcan_Bot2011_IGS	NEWB9	1	BC37463_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37463	Sample	BC37463	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB9	IGS	45	BC37463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB9	CGTAATCCTCAGTACTGGCTA	151.77235
BC37463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90467	WGS84	BC37463	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37463_Sspa_Oph2007_COI	NEWB9	1	BC37463_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37463	Sample	BC37463	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB9	COI	45	BC37463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB9	GAAGGCCTTCCGATGATC	151.77235
BC37463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90467	WGS84	BC37463	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37463_Upin_Bot2011_COI	NEWB9	1	BC37463_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37463	Sample	BC37463	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB9	COI	45	BC37463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB9	CTTTAATTACAGCGTTTTTATTGTTGT	151.77235
BC37464	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.90649	WGS84	BC37464	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.821	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37464_Mgig_Bot2012_COI	NEWB8	1	BC37464_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37464	Sample	BC37464	targeted	Present	Sample	Mgig_Bot2012_COI	NEWB8	COI	45	BC37464	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB8	TCTTATTCGTTGGAGACTTTATAACCCT	151.77151
BC37465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91934	WGS84	BC37465	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37465_Cmae_Bot2011_COI	NEWB7	1	BC37465_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37465	Sample	BC37465	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB7	COI	45	BC37465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB7	ATGAACAGTCTATCCTCCTTTAG	151.76933
BC37465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.91934	WGS84	BC37465	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37465_Msal_Bot2012_COI	NEWB7	1	BC37465_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37465	Sample	BC37465	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB7	COI	45	BC37465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB7	GYTAGTTCCRATGATGTTAGCTG	151.76933
BC37467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.9216	WGS84	BC37467	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37467_Aamu_Bax2006_COI	NEWB5	1	BC37467_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37467	Sample	BC37467	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB5	COI	45	BC37467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB5	GCACAACCGGGATCTTTACTTC	151.76876
BC37467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.9216	WGS84	BC37467	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37467_Pcan_Bot2011_IGS	NEWB5	1	BC37467_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37467	Sample	BC37467	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB5	IGS	45	BC37467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB5	CGTAATCCTCAGTACTGGCTA	151.76876
BC37467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.9216	WGS84	BC37467	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37467_Sspa_Oph2007_COI	NEWB5	1	BC37467_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37467	Sample	BC37467	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB5	COI	45	BC37467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB5	GAAGGCCTTCCGATGATC	151.76876
BC37467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.9216	WGS84	BC37467	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37467_Upin_Bot2011_COI	NEWB5	1	BC37467_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37467	Sample	BC37467	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB5	COI	45	BC37467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB5	CTTTAATTACAGCGTTTTTATTGTTGT	151.76876
BC37468	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92383	WGS84	BC37468	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37468_Mgig_Bot2012_COI	NEWB4	1	BC37468_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37468	Sample	BC37468	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB4	COI	45	BC37468	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB4	TCTTATTCGTTGGAGACTTTATAACCCT	151.77345
BC37469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92421	WGS84	BC37469	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37469_Cmae_Bot2011_COI	NEWB3	1	BC37469_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37469	Sample	BC37469	targeted	Absent	Sample	Cmae_Bot2011_COI	NEWB3	COI	45	BC37469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB3	ATGAACAGTCTATCCTCCTTTAG	151.77064
BC37469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92421	WGS84	BC37469	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37469_Msal_Bot2012_COI	NEWB3	1	BC37469_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37469	Sample	BC37469	targeted	Absent	Sample	Msal_Bot2012_COI	NEWB3	COI	45	BC37469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB3	GYTAGTTCCRATGATGTTAGCTG	151.77064
BC37470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92384	WGS84	BC37470	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37470_Aamu_Bax2006_COI	NEWB2	1	BC37470_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37470	Sample	BC37470	targeted	Absent	Sample	Aamu_Bax2006_COI	NEWB2	COI	45	BC37470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB2	GCACAACCGGGATCTTTACTTC	151.76765
BC37470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92384	WGS84	BC37470	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37470_Pcan_Bot2011_IGS	NEWB2	1	BC37470_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37470	Sample	BC37470	targeted	Absent	Sample	Pcan_Bot2011_IGS	NEWB2	IGS	45	BC37470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB2	CGTAATCCTCAGTACTGGCTA	151.76765
BC37470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92384	WGS84	BC37470	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37470_Sspa_Oph2007_COI	NEWB2	1	BC37470_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37470	Sample	BC37470	targeted	Absent	Sample	Sspa_Oph2007_COI	NEWB2	COI	45	BC37470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB2	GAAGGCCTTCCGATGATC	151.76765
BC37470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92384	WGS84	BC37470	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37470_Upin_Bot2011_COI	NEWB2	1	BC37470_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37470	Sample	BC37470	targeted	Absent	Sample	Upin_Bot2011_COI	NEWB2	COI	45	BC37470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB2	CTTTAATTACAGCGTTTTTATTGTTGT	151.76765
BC37471	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-32.92394	WGS84	BC37471	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Newcastle_Autumn	15/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37471_Mgig_Bot2012_COI	NEWB1	1	BC37471_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37471	Sample	BC37471	targeted	Absent	Sample	Mgig_Bot2012_COI	NEWB1	COI	45	BC37471	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	NEWB1	TCTTATTCGTTGGAGACTTTATAACCCT	151.76439
BC37472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00231	WGS84	BC37472	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37472_Cmae_Bot2011_COI	BOTB1	1	BC37472_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37472	Sample	BC37472	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB1	COI	45	BC37472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB1	ATGAACAGTCTATCCTCCTTTAG	151.20921
BC37472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00231	WGS84	BC37472	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	19/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37472_Msal_Bot2012_COI	BOTB1	1	BC37472_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37472	Sample	BC37472	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB1	COI	45	BC37472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB1	GYTAGTTCCRATGATGTTAGCTG	151.20921
BC37474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00023	WGS84	BC37474	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37474_Aamu_Bax2006_COI	BOTB3	1	BC37474_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37474	Sample	BC37474	targeted	Absent	Sample	Aamu_Bax2006_COI	BOTB3	COI	45	BC37474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB3	GCACAACCGGGATCTTTACTTC	151.20815
BC37474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00023	WGS84	BC37474	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37474_Pcan_Bot2011_IGS	BOTB3	1	BC37474_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37474	Sample	BC37474	targeted	Absent	Sample	Pcan_Bot2011_IGS	BOTB3	IGS	45	BC37474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB3	CGTAATCCTCAGTACTGGCTA	151.20815
BC37474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00023	WGS84	BC37474	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37474_Sspa_Oph2007_COI	BOTB3	1	BC37474_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37474	Sample	BC37474	targeted	Absent	Sample	Sspa_Oph2007_COI	BOTB3	COI	45	BC37474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB3	GAAGGCCTTCCGATGATC	151.20815
BC37474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-34.00023	WGS84	BC37474	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37474_Upin_Bot2011_COI	BOTB3	1	BC37474_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37474	Sample	BC37474	targeted	Absent	Sample	Upin_Bot2011_COI	BOTB3	COI	45	BC37474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB3	CTTTAATTACAGCGTTTTTATTGTTGT	151.20815
BC37475	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99948	WGS84	BC37475	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37475_Mgig_Bot2012_COI	BOTB4	1	BC37475_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37475	Sample	BC37475	targeted	Absent	Sample	Mgig_Bot2012_COI	BOTB4	COI	45	BC37475	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB4	TCTTATTCGTTGGAGACTTTATAACCCT	151.21169
BC37476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99864	WGS84	BC37476	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37476_Cmae_Bot2011_COI	BOTB5	1	BC37476_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37476	Sample	BC37476	targeted	Absent	Sample	Cmae_Bot2011_COI	BOTB5	COI	45	BC37476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB5	ATGAACAGTCTATCCTCCTTTAG	151.20885
BC37476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12231	-33.99864	WGS84	BC37476	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Botany_Autumn	20/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37476_Msal_Bot2012_COI	BOTB5	1	BC37476_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37476	Sample	BC37476	targeted	Absent	Sample	Msal_Bot2012_COI	BOTB5	COI	45	BC37476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BOTB5	GYTAGTTCCRATGATGTTAGCTG	151.20885
BC37478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45463	WGS84	BC37478	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37478_Aamu_Bax2006_COI	KEMB34	1	BC37478_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37478	Sample	BC37478	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB34	COI	45	BC37478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB34	GCACAACCGGGATCTTTACTTC	150.89491
BC37478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45463	WGS84	BC37478	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37478_Pcan_Bot2011_IGS	KEMB34	1	BC37478_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37478	Sample	BC37478	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB34	IGS	45	BC37478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB34	CGTAATCCTCAGTACTGGCTA	150.89491
BC37478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45463	WGS84	BC37478	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37478_Sspa_Oph2007_COI	KEMB34	1	BC37478_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37478	Sample	BC37478	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB34	COI	45	BC37478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB34	GAAGGCCTTCCGATGATC	150.89491
BC37478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45463	WGS84	BC37478	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37478_Upin_Bot2011_COI	KEMB34	1	BC37478_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37478	Sample	BC37478	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB34	COI	45	BC37478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB34	CTTTAATTACAGCGTTTTTATTGTTGT	150.89491
BC37479	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45485	WGS84	BC37479	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.165	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37479_Mgig_Bot2012_COI	KEMB33	1	BC37479_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37479	Sample	BC37479	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB33	COI	45	BC37479	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB33	TCTTATTCGTTGGAGACTTTATAACCCT	150.8876
BC37481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45662	WGS84	BC37481	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37481_Aamu_Bax2006_COI	KEMB31	1	BC37481_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37481	Sample	BC37481	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB31	COI	45	BC37481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB31	GCACAACCGGGATCTTTACTTC	150.89192
BC37481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45662	WGS84	BC37481	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37481_Pcan_Bot2011_IGS	KEMB31	1	BC37481_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37481	Sample	BC37481	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB31	IGS	45	BC37481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB31	CGTAATCCTCAGTACTGGCTA	150.89192
BC37481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45662	WGS84	BC37481	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37481_Sspa_Oph2007_COI	KEMB31	1	BC37481_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37481	Sample	BC37481	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB31	COI	45	BC37481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB31	GAAGGCCTTCCGATGATC	150.89192
BC37481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45662	WGS84	BC37481	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37481_Upin_Bot2011_COI	KEMB31	1	BC37481_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37481	Sample	BC37481	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB31	COI	45	BC37481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB31	CTTTAATTACAGCGTTTTTATTGTTGT	150.89192
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37482_Asen_Wil2025_COI	KEMB30	1	BC37482_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	GTTGTTGTTACAACACATGCTCTTTTG	150.88835
BC37482	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45677	WGS84	BC37482	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37482_Mgig_Bot2012_COI	KEMB30	1	BC37482_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37482	Sample	BC37482	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB30	COI	45	BC37482	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB30	TCTTATTCGTTGGAGACTTTATAACCCT	150.88835
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37483_Cmae_Bot2011_COI	KEMB29	1	BC37483_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	ATGAACAGTCTATCCTCCTTTAG	150.89545
BC37483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45771	WGS84	BC37483	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37483_Msal_Bot2012_COI	KEMB29	1	BC37483_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37483	Sample	BC37483	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB29	COI	45	BC37483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB29	GYTAGTTCCRATGATGTTAGCTG	150.89545
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37485_Aamu_Bax2006_COI	KEMB27	1	BC37485_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	GCACAACCGGGATCTTTACTTC	150.89094
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37485_Pcan_Bot2011_IGS	KEMB27	1	BC37485_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB27	IGS	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	CGTAATCCTCAGTACTGGCTA	150.89094
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37485_Sspa_Oph2007_COI	KEMB27	1	BC37485_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	GAAGGCCTTCCGATGATC	150.89094
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37485_Upin_Bot2011_COI	KEMB27	1	BC37485_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	CTTTAATTACAGCGTTTTTATTGTTGT	150.89094
BC37485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45875	WGS84	BC37485	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37485_Vgib_Wil2025_COI	KEMB27	1	BC37485_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37485	Sample	BC37485	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB27	COI	45	BC37485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB27	CCCCTCTTTACAAGTTCTTTAGGGT	150.89094
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC37486_Asen_Wil2025_COI	KEMB26	1	BC37486_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Absent	Sample	Asen_Wil2025_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	GTTGTTGTTACAACACATGCTCTTTTG	150.89653
BC37486	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.45989	WGS84	BC37486	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.616	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37486_Mgig_Bot2012_COI	KEMB26	1	BC37486_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37486	Sample	BC37486	targeted	Present	Sample	Mgig_Bot2012_COI	KEMB26	COI	45	BC37486	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB26	TCTTATTCGTTGGAGACTTTATAACCCT	150.89653
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37487_Cmae_Bot2011_COI	KEMB25	1	BC37487_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	ATGAACAGTCTATCCTCCTTTAG	150.89433
BC37487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46003	WGS84	BC37487	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37487_Msal_Bot2012_COI	KEMB25	1	BC37487_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37487	Sample	BC37487	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB25	COI	45	BC37487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB25	GYTAGTTCCRATGATGTTAGCTG	150.89433
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37489_Aamu_Bax2006_COI	KEMB23	1	BC37489_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	GCACAACCGGGATCTTTACTTC	150.89793
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37489_Pcan_Bot2011_IGS	KEMB23	1	BC37489_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB23	IGS	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	CGTAATCCTCAGTACTGGCTA	150.89793
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37489_Sspa_Oph2007_COI	KEMB23	1	BC37489_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	GAAGGCCTTCCGATGATC	150.89793
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37489_Upin_Bot2011_COI	KEMB23	1	BC37489_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	CTTTAATTACAGCGTTTTTATTGTTGT	150.89793
BC37489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46132	WGS84	BC37489	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC37489_Vgib_Wil2025_COI	KEMB23	1	BC37489_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37489	Sample	BC37489	targeted	Absent	Sample	Vgib_Wil2025_COI	KEMB23	COI	45	BC37489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB23	CCCCTCTTTACAAGTTCTTTAGGGT	150.89793
BC37490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46159	WGS84	BC37490	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37490_Cmae_Bot2011_COI	KEMB22	1	BC37490_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37490	Sample	BC37490	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB22	COI	45	BC37490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB22	ATGAACAGTCTATCCTCCTTTAG	150.89548
BC37490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46159	WGS84	BC37490	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37490_Msal_Bot2012_COI	KEMB22	1	BC37490_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37490	Sample	BC37490	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB22	COI	45	BC37490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB22	GYTAGTTCCRATGATGTTAGCTG	150.89548
BC37492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46283	WGS84	BC37492	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37492_Aamu_Bax2006_COI	KEMB20	1	BC37492_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37492	Sample	BC37492	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB20	COI	45	BC37492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB20	GCACAACCGGGATCTTTACTTC	150.89739
BC37492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46283	WGS84	BC37492	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37492_Pcan_Bot2011_IGS	KEMB20	1	BC37492_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37492	Sample	BC37492	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB20	IGS	45	BC37492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB20	CGTAATCCTCAGTACTGGCTA	150.89739
BC37492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46283	WGS84	BC37492	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37492_Sspa_Oph2007_COI	KEMB20	1	BC37492_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37492	Sample	BC37492	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB20	COI	45	BC37492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB20	GAAGGCCTTCCGATGATC	150.89739
BC37492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46283	WGS84	BC37492	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37492_Upin_Bot2011_COI	KEMB20	1	BC37492_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37492	Sample	BC37492	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB20	COI	45	BC37492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB20	CTTTAATTACAGCGTTTTTATTGTTGT	150.89739
BC37493	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46383	WGS84	BC37493	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37493_Mgig_Bot2012_COI	KEMB19	1	BC37493_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37493	Sample	BC37493	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB19	COI	45	BC37493	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB19	TCTTATTCGTTGGAGACTTTATAACCCT	150.90512
BC37494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46358	WGS84	BC37494	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37494_Cmae_Bot2011_COI	KEMB18	1	BC37494_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37494	Sample	BC37494	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB18	COI	45	BC37494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB18	ATGAACAGTCTATCCTCCTTTAG	150.90353
BC37494	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46358	WGS84	BC37494	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37494_Msal_Bot2012_COI	KEMB18	1	BC37494_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37494	Sample	BC37494	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB18	COI	45	BC37494	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB18	GYTAGTTCCRATGATGTTAGCTG	150.90353
BC37496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46441	WGS84	BC37496	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37496_Aamu_Bax2006_COI	KEMB16	1	BC37496_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37496	Sample	BC37496	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB16	COI	45	BC37496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB16	GCACAACCGGGATCTTTACTTC	150.9084
BC37496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46441	WGS84	BC37496	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37496_Pcan_Bot2011_IGS	KEMB16	1	BC37496_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37496	Sample	BC37496	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB16	IGS	45	BC37496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB16	CGTAATCCTCAGTACTGGCTA	150.9084
BC37496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46441	WGS84	BC37496	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37496_Sspa_Oph2007_COI	KEMB16	1	BC37496_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37496	Sample	BC37496	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB16	COI	45	BC37496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB16	GAAGGCCTTCCGATGATC	150.9084
BC37496	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46441	WGS84	BC37496	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37496_Upin_Bot2011_COI	KEMB16	1	BC37496_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37496	Sample	BC37496	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB16	COI	45	BC37496	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB16	CTTTAATTACAGCGTTTTTATTGTTGT	150.9084
BC37497	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46504	WGS84	BC37497	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37497_Mgig_Bot2012_COI	KEMB15	1	BC37497_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37497	Sample	BC37497	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB15	COI	45	BC37497	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB15	TCTTATTCGTTGGAGACTTTATAACCCT	150.90708
BC37498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46521	WGS84	BC37498	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37498_Cmae_Bot2011_COI	KEMB14	1	BC37498_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37498	Sample	BC37498	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB14	COI	45	BC37498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB14	ATGAACAGTCTATCCTCCTTTAG	150.90487
BC37498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46521	WGS84	BC37498	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37498_Msal_Bot2012_COI	KEMB14	1	BC37498_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37498	Sample	BC37498	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB14	COI	45	BC37498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB14	GYTAGTTCCRATGATGTTAGCTG	150.90487
BC37500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46622	WGS84	BC37500	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37500_Aamu_Bax2006_COI	KEMB12	1	BC37500_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37500	Sample	BC37500	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB12	COI	45	BC37500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB12	GCACAACCGGGATCTTTACTTC	150.90847
BC37500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46622	WGS84	BC37500	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37500_Pcan_Bot2011_IGS	KEMB12	1	BC37500_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37500	Sample	BC37500	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB12	IGS	45	BC37500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB12	CGTAATCCTCAGTACTGGCTA	150.90847
BC37500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46622	WGS84	BC37500	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37500_Sspa_Oph2007_COI	KEMB12	1	BC37500_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37500	Sample	BC37500	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB12	COI	45	BC37500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB12	GAAGGCCTTCCGATGATC	150.90847
BC37500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46622	WGS84	BC37500	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37500_Upin_Bot2011_COI	KEMB12	1	BC37500_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37500	Sample	BC37500	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB12	COI	45	BC37500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB12	CTTTAATTACAGCGTTTTTATTGTTGT	150.90847
BC37501	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46709	WGS84	BC37501	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	14/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37501_Mgig_Bot2012_COI	KEMB11	1	BC37501_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37501	Sample	BC37501	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB11	COI	45	BC37501	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB11	TCTTATTCGTTGGAGACTTTATAACCCT	150.90581
BC37502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46689	WGS84	BC37502	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37502_Cmae_Bot2011_COI	KEMB10	1	BC37502_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37502	Sample	BC37502	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB10	COI	45	BC37502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB10	ATGAACAGTCTATCCTCCTTTAG	150.90398
BC37502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46689	WGS84	BC37502	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37502_Msal_Bot2012_COI	KEMB10	1	BC37502_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37502	Sample	BC37502	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB10	COI	45	BC37502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB10	GYTAGTTCCRATGATGTTAGCTG	150.90398
BC37504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46864	WGS84	BC37504	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC37504_Aamu_Bax2006_COI	KEMB8	1	BC37504_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37504	Sample	BC37504	targeted	Absent	Sample	Aamu_Bax2006_COI	KEMB8	COI	45	BC37504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB8	GCACAACCGGGATCTTTACTTC	150.90694
BC37504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46864	WGS84	BC37504	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC37504_Pcan_Bot2011_IGS	KEMB8	1	BC37504_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37504	Sample	BC37504	targeted	Absent	Sample	Pcan_Bot2011_IGS	KEMB8	IGS	45	BC37504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB8	CGTAATCCTCAGTACTGGCTA	150.90694
BC37504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46864	WGS84	BC37504	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC37504_Sspa_Oph2007_COI	KEMB8	1	BC37504_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37504	Sample	BC37504	targeted	Absent	Sample	Sspa_Oph2007_COI	KEMB8	COI	45	BC37504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB8	GAAGGCCTTCCGATGATC	150.90694
BC37504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46864	WGS84	BC37504	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC37504_Upin_Bot2011_COI	KEMB8	1	BC37504_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37504	Sample	BC37504	targeted	Absent	Sample	Upin_Bot2011_COI	KEMB8	COI	45	BC37504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB8	CTTTAATTACAGCGTTTTTATTGTTGT	150.90694
BC37505	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.46901	WGS84	BC37505	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC37505_Mgig_Bot2012_COI	KEMB7	1	BC37505_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37505	Sample	BC37505	targeted	Absent	Sample	Mgig_Bot2012_COI	KEMB7	COI	45	BC37505	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB7	TCTTATTCGTTGGAGACTTTATAACCCT	150.90504
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC37506_Cmae_Bot2011_COI	KEMB6	1	BC37506_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Cmae_Bot2011_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	ATGAACAGTCTATCCTCCTTTAG	150.90624
BC37506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12229	-34.47012	WGS84	BC37506	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2019) Molecular surveys for priority marine pests in 2018-2019 for the ports of Gove, Weipa, Hay Point, Newcastle, Botany Bay, Port Kembla and Devonport. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2019/000406-1. SARDI Research Report Series No. 1044., Adelaide.	2018-19_Molecular_surveys-Port_Kembla_Autumn	13/05/2019	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC37506_Msal_Bot2012_COI	KEMB6	1	BC37506_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC37506	Sample	BC37506	targeted	Absent	Sample	Msal_Bot2012_COI	KEMB6	COI	45	BC37506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KEMB6	GYTAGTTCCRATGATGTTAGCTG	150.90624
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63715_Cjap_Sim2018_COI	FREB30	1	BC63715_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74996
BC63715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04111	WGS84	BC63715	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63715_Mgig_Bot2012_COI	FREB30	1	BC63715_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63715	Sample	BC63715	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB30	COI	45	BC63715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB30	TCTTATTCGTTGGAGACTTTATAACCCT	115.74996
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63716_Cmae_Bot2011_COI	FREB25	1	BC63716_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	ATGAACAGTCTATCCTCCTTTAG	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63716_Msal_Bot2012_COI	FREB25	1	BC63716_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Msal_Bot2012_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	GYTAGTTCCRATGATGTTAGCTG	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63716_Pvir_Dia2013_COI	FREB25	1	BC63716_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	CTTAGTGGCATTAATTCGDAATCC	115.74773
BC63716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04333	WGS84	BC63716	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63716_Rhar_Sim2018_COI	FREB25	1	BC63716_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63716	Sample	BC63716	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB25	COI	45	BC63716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB25	CTGCTGCTATTGCTCATG	115.74773
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63718_Aamu_Bax2006_COI	FREB15	1	BC63718_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	GCACAACCGGGATCTTTACTTC	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63718_Pcan_Bot2011_IGS	FREB15	1	BC63718_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB15	IGS	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	CGTAATCCTCAGTACTGGCTA	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63718_Pper_Dia2013_COI	FREB15	1	BC63718_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Pper_Dia2013_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	CTTAGTGGCATTAATTCGDAATCC	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63718_Sspa_Oph2007_COI	FREB15	1	BC63718_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	GAAGGCCTTCCGATGATC	115.74405
BC63718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04758	WGS84	BC63718	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63718_Upin_Bot2011_COI	FREB15	1	BC63718_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63718	Sample	BC63718	targeted	Absent	Sample	Upin_Bot2011_COI	FREB15	COI	45	BC63718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB15	CTTTAATTACAGCGTTTTTATTGTTGT	115.74405
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63719_Cjap_Sim2018_COI	FREB10	1	BC63719_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74163
BC63719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04948	WGS84	BC63719	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63719_Mgig_Bot2012_COI	FREB10	1	BC63719_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63719	Sample	BC63719	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB10	COI	45	BC63719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB10	TCTTATTCGTTGGAGACTTTATAACCCT	115.74163
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63721_Aamu_Bax2006_COI	FREB33	1	BC63721_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	GCACAACCGGGATCTTTACTTC	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63721_Pcan_Bot2011_IGS	FREB33	1	BC63721_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB33	IGS	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	CGTAATCCTCAGTACTGGCTA	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63721_Pper_Dia2013_COI	FREB33	1	BC63721_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Pper_Dia2013_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	CTTAGTGGCATTAATTCGDAATCC	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63721_Sspa_Oph2007_COI	FREB33	1	BC63721_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	GAAGGCCTTCCGATGATC	115.75105
BC63721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04146	WGS84	BC63721	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63721_Upin_Bot2011_COI	FREB33	1	BC63721_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63721	Sample	BC63721	targeted	Absent	Sample	Upin_Bot2011_COI	FREB33	COI	45	BC63721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB33	CTTTAATTACAGCGTTTTTATTGTTGT	115.75105
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63722_Cjap_Sim2018_COI	FREB32	1	BC63722_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7509
BC63722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04079	WGS84	BC63722	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63722_Mgig_Bot2012_COI	FREB32	1	BC63722_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63722	Sample	BC63722	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB32	COI	45	BC63722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB32	TCTTATTCGTTGGAGACTTTATAACCCT	115.7509
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63723_Cmae_Bot2011_COI	FREB31	1	BC63723_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	ATGAACAGTCTATCCTCCTTTAG	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63723_Msal_Bot2012_COI	FREB31	1	BC63723_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Msal_Bot2012_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	GYTAGTTCCRATGATGTTAGCTG	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63723_Pvir_Dia2013_COI	FREB31	1	BC63723_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	CTTAGTGGCATTAATTCGDAATCC	115.75126
BC63723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0412	WGS84	BC63723	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63723_Rhar_Sim2018_COI	FREB31	1	BC63723_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63723	Sample	BC63723	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB31	COI	45	BC63723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB31	CTGCTGCTATTGCTCATG	115.75126
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63725_Aamu_Bax2006_COI	FREB28	1	BC63725_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	GCACAACCGGGATCTTTACTTC	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63725_Pcan_Bot2011_IGS	FREB28	1	BC63725_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB28	IGS	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	CGTAATCCTCAGTACTGGCTA	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63725_Pper_Dia2013_COI	FREB28	1	BC63725_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Pper_Dia2013_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	CTTAGTGGCATTAATTCGDAATCC	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63725_Sspa_Oph2007_COI	FREB28	1	BC63725_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	GAAGGCCTTCCGATGATC	115.74897
BC63725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0423	WGS84	BC63725	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63725_Upin_Bot2011_COI	FREB28	1	BC63725_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63725	Sample	BC63725	targeted	Absent	Sample	Upin_Bot2011_COI	FREB28	COI	45	BC63725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB28	CTTTAATTACAGCGTTTTTATTGTTGT	115.74897
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63726_Cjap_Sim2018_COI	FREB27	1	BC63726_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74747
BC63726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04263	WGS84	BC63726	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63726_Mgig_Bot2012_COI	FREB27	1	BC63726_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63726	Sample	BC63726	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB27	COI	45	BC63726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB27	TCTTATTCGTTGGAGACTTTATAACCCT	115.74747
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63727_Cmae_Bot2011_COI	FREB26	1	BC63727_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	ATGAACAGTCTATCCTCCTTTAG	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63727_Msal_Bot2012_COI	FREB26	1	BC63727_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Msal_Bot2012_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	GYTAGTTCCRATGATGTTAGCTG	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63727_Pvir_Dia2013_COI	FREB26	1	BC63727_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	CTTAGTGGCATTAATTCGDAATCC	115.74931
BC63727	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04346	WGS84	BC63727	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63727_Rhar_Sim2018_COI	FREB26	1	BC63727_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63727	Sample	BC63727	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB26	COI	45	BC63727	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB26	CTGCTGCTATTGCTCATG	115.74931
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63729_Aamu_Bax2006_COI	FREB23	1	BC63729_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	GCACAACCGGGATCTTTACTTC	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63729_Pcan_Bot2011_IGS	FREB23	1	BC63729_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB23	IGS	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	CGTAATCCTCAGTACTGGCTA	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63729_Pper_Dia2013_COI	FREB23	1	BC63729_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Pper_Dia2013_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	CTTAGTGGCATTAATTCGDAATCC	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63729_Sspa_Oph2007_COI	FREB23	1	BC63729_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	GAAGGCCTTCCGATGATC	115.74823
BC63729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04445	WGS84	BC63729	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63729_Upin_Bot2011_COI	FREB23	1	BC63729_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63729	Sample	BC63729	targeted	Absent	Sample	Upin_Bot2011_COI	FREB23	COI	45	BC63729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB23	CTTTAATTACAGCGTTTTTATTGTTGT	115.74823
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63730_Cmae_Bot2011_COI	FREB22	1	BC63730_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	ATGAACAGTCTATCCTCCTTTAG	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63730_Msal_Bot2012_COI	FREB22	1	BC63730_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Msal_Bot2012_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	GYTAGTTCCRATGATGTTAGCTG	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63730_Pvir_Dia2013_COI	FREB22	1	BC63730_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	CTTAGTGGCATTAATTCGDAATCC	115.74684
BC63730	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04436	WGS84	BC63730	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63730_Rhar_Sim2018_COI	FREB22	1	BC63730_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63730	Sample	BC63730	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB22	COI	45	BC63730	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB22	CTGCTGCTATTGCTCATG	115.74684
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63732_Aamu_Bax2006_COI	FREB19	1	BC63732_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	GCACAACCGGGATCTTTACTTC	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63732_Pcan_Bot2011_IGS	FREB19	1	BC63732_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB19	IGS	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	CGTAATCCTCAGTACTGGCTA	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63732_Pper_Dia2013_COI	FREB19	1	BC63732_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Pper_Dia2013_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	CTTAGTGGCATTAATTCGDAATCC	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63732_Sspa_Oph2007_COI	FREB19	1	BC63732_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	GAAGGCCTTCCGATGATC	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04564	WGS84	BC63732	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63732_Upin_Bot2011_COI	FREB19	1	BC63732_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Upin_Bot2011_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	CTTTAATTACAGCGTTTTTATTGTTGT	115.74577
BC63732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04564	WGS84	BC63732	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63732_Vgib_Wil2025_COI	FREB19	1	BC63732_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63732	Sample	BC63732	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB19	COI	45	BC63732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB19	CCCCTCTTTACAAGTTCTTTAGGGT	115.74577
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04584	WGS84	BC63733	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	25.059	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63733_Asen_Wil2025_COI	FREB18	1	BC63733_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Present	Sample	Asen_Wil2025_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	GTTGTTGTTACAACACATGCTCTTTTG	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63733_Cjap_Sim2018_COI	FREB18	1	BC63733_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74461
BC63733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04584	WGS84	BC63733	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63733_Mgig_Bot2012_COI	FREB18	1	BC63733_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63733	Sample	BC63733	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB18	COI	45	BC63733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB18	TCTTATTCGTTGGAGACTTTATAACCCT	115.74461
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63734_Cmae_Bot2011_COI	FREB17	1	BC63734_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	ATGAACAGTCTATCCTCCTTTAG	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63734_Msal_Bot2012_COI	FREB17	1	BC63734_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Msal_Bot2012_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	GYTAGTTCCRATGATGTTAGCTG	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63734_Pvir_Dia2013_COI	FREB17	1	BC63734_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	CTTAGTGGCATTAATTCGDAATCC	115.74626
BC63734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04686	WGS84	BC63734	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63734_Rhar_Sim2018_COI	FREB17	1	BC63734_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63734	Sample	BC63734	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB17	COI	45	BC63734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB17	CTGCTGCTATTGCTCATG	115.74626
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63736_Aamu_Bax2006_COI	FREB14	1	BC63736_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	GCACAACCGGGATCTTTACTTC	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63736_Pcan_Bot2011_IGS	FREB14	1	BC63736_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB14	IGS	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	CGTAATCCTCAGTACTGGCTA	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63736_Pper_Dia2013_COI	FREB14	1	BC63736_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Pper_Dia2013_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	CTTAGTGGCATTAATTCGDAATCC	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63736_Sspa_Oph2007_COI	FREB14	1	BC63736_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	GAAGGCCTTCCGATGATC	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04787	WGS84	BC63736	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63736_Upin_Bot2011_COI	FREB14	1	BC63736_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Upin_Bot2011_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	CTTTAATTACAGCGTTTTTATTGTTGT	115.74513
BC63736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04787	WGS84	BC63736	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63736_Vgib_Wil2025_COI	FREB14	1	BC63736_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63736	Sample	BC63736	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB14	COI	45	BC63736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB14	CCCCTCTTTACAAGTTCTTTAGGGT	115.74513
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.04791	WGS84	BC63737	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	25.357	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63737_Asen_Wil2025_COI	FREB13	1	BC63737_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Present	Sample	Asen_Wil2025_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	GTTGTTGTTACAACACATGCTCTTTTG	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63737_Cjap_Sim2018_COI	FREB13	1	BC63737_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74378
BC63737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04791	WGS84	BC63737	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63737_Mgig_Bot2012_COI	FREB13	1	BC63737_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63737	Sample	BC63737	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB13	COI	45	BC63737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB13	TCTTATTCGTTGGAGACTTTATAACCCT	115.74378
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63738_Cmae_Bot2011_COI	FREB12	1	BC63738_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	ATGAACAGTCTATCCTCCTTTAG	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63738_Msal_Bot2012_COI	FREB12	1	BC63738_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Msal_Bot2012_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	GYTAGTTCCRATGATGTTAGCTG	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63738_Pvir_Dia2013_COI	FREB12	1	BC63738_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	CTTAGTGGCATTAATTCGDAATCC	115.74316
BC63738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.04822	WGS84	BC63738	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63738_Rhar_Sim2018_COI	FREB12	1	BC63738_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63738	Sample	BC63738	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB12	COI	45	BC63738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB12	CTGCTGCTATTGCTCATG	115.74316
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.0496	WGS84	BC63740	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	23.972	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63740_Asen_Wil2025_COI	FREB9	1	BC63740_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Present	Sample	Asen_Wil2025_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	GTTGTTGTTACAACACATGCTCTTTTG	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63740_Cjap_Sim2018_COI	FREB9	1	BC63740_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74163
BC63740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.0496	WGS84	BC63740	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63740_Mgig_Bot2012_COI	FREB9	1	BC63740_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63740	Sample	BC63740	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB9	COI	45	BC63740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB9	TCTTATTCGTTGGAGACTTTATAACCCT	115.74163
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63741_Cmae_Bot2011_COI	FREB8	1	BC63741_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	ATGAACAGTCTATCCTCCTTTAG	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63741_Msal_Bot2012_COI	FREB8	1	BC63741_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Msal_Bot2012_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	GYTAGTTCCRATGATGTTAGCTG	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63741_Pvir_Dia2013_COI	FREB8	1	BC63741_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	CTTAGTGGCATTAATTCGDAATCC	115.74305
BC63741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05009	WGS84	BC63741	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63741_Rhar_Sim2018_COI	FREB8	1	BC63741_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63741	Sample	BC63741	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB8	COI	45	BC63741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB8	CTGCTGCTATTGCTCATG	115.74305
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63743_Aamu_Bax2006_COI	FREB6	1	BC63743_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	GCACAACCGGGATCTTTACTTC	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63743_Pcan_Bot2011_IGS	FREB6	1	BC63743_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB6	IGS	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	CGTAATCCTCAGTACTGGCTA	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63743_Pper_Dia2013_COI	FREB6	1	BC63743_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Pper_Dia2013_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	CTTAGTGGCATTAATTCGDAATCC	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63743_Sspa_Oph2007_COI	FREB6	1	BC63743_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	GAAGGCCTTCCGATGATC	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05114	WGS84	BC63743	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63743_Upin_Bot2011_COI	FREB6	1	BC63743_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Upin_Bot2011_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	CTTTAATTACAGCGTTTTTATTGTTGT	115.74209
BC63743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05114	WGS84	BC63743	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63743_Vgib_Wil2025_COI	FREB6	1	BC63743_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63743	Sample	BC63743	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB6	COI	45	BC63743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB6	CCCCTCTTTACAAGTTCTTTAGGGT	115.74209
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05213	WGS84	BC63744	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	25.338	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63744_Asen_Wil2025_COI	FREB4	1	BC63744_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Present	Sample	Asen_Wil2025_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	GTTGTTGTTACAACACATGCTCTTTTG	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63744_Cjap_Sim2018_COI	FREB4	1	BC63744_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Cjap_Sim2018_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74122
BC63744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05213	WGS84	BC63744	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63744_Mgig_Bot2012_COI	FREB4	1	BC63744_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63744	Sample	BC63744	targeted	Absent	Sample	Mgig_Bot2012_COI	FREB4	COI	45	BC63744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB4	TCTTATTCGTTGGAGACTTTATAACCCT	115.74122
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63745_Cmae_Bot2011_COI	FREB3	1	BC63745_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Cmae_Bot2011_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	ATGAACAGTCTATCCTCCTTTAG	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63745_Msal_Bot2012_COI	FREB3	1	BC63745_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Msal_Bot2012_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	GYTAGTTCCRATGATGTTAGCTG	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63745_Pvir_Dia2013_COI	FREB3	1	BC63745_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Pvir_Dia2013_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	CTTAGTGGCATTAATTCGDAATCC	115.73963
BC63745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05217	WGS84	BC63745	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63745_Rhar_Sim2018_COI	FREB3	1	BC63745_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63745	Sample	BC63745	targeted	Absent	Sample	Rhar_Sim2018_COI	FREB3	COI	45	BC63745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB3	CTGCTGCTATTGCTCATG	115.73963
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63747_Aamu_Bax2006_COI	FREB1	1	BC63747_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Aamu_Bax2006_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	GCACAACCGGGATCTTTACTTC	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63747_Pcan_Bot2011_IGS	FREB1	1	BC63747_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREB1	IGS	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	CGTAATCCTCAGTACTGGCTA	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63747_Pper_Dia2013_COI	FREB1	1	BC63747_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Pper_Dia2013_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	CTTAGTGGCATTAATTCGDAATCC	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63747_Sspa_Oph2007_COI	FREB1	1	BC63747_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Sspa_Oph2007_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	GAAGGCCTTCCGATGATC	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-32.05427	WGS84	BC63747	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63747_Upin_Bot2011_COI	FREB1	1	BC63747_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Upin_Bot2011_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	CTTTAATTACAGCGTTTTTATTGTTGT	115.73746
BC63747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.05427	WGS84	BC63747	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Fremantle_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63747_Vgib_Wil2025_COI	FREB1	1	BC63747_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63747	Sample	BC63747	targeted	Absent	Sample	Vgib_Wil2025_COI	FREB1	COI	45	BC63747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREB1	CCCCTCTTTACAAGTTCTTTAGGGT	115.73746
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63748_Cjap_Sim2018_COI	KWIB60	1	BC63748_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77273
BC63748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18686	WGS84	BC63748	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63748_Mgig_Bot2012_COI	KWIB60	1	BC63748_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63748	Sample	BC63748	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB60	COI	45	BC63748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB60	TCTTATTCGTTGGAGACTTTATAACCCT	115.77273
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63749_Cmae_Bot2011_COI	KWIB55	1	BC63749_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	ATGAACAGTCTATCCTCCTTTAG	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63749_Msal_Bot2012_COI	KWIB55	1	BC63749_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	GYTAGTTCCRATGATGTTAGCTG	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63749_Pvir_Dia2013_COI	KWIB55	1	BC63749_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	CTTAGTGGCATTAATTCGDAATCC	115.77353
BC63749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19163	WGS84	BC63749	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63749_Rhar_Sim2018_COI	KWIB55	1	BC63749_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63749	Sample	BC63749	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB55	COI	45	BC63749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB55	CTGCTGCTATTGCTCATG	115.77353
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63750_Aamu_Bax2006_COI	KWIB50	1	BC63750_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	GCACAACCGGGATCTTTACTTC	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63750_Pcan_Bot2011_IGS	KWIB50	1	BC63750_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB50	IGS	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	CGTAATCCTCAGTACTGGCTA	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63750_Pper_Dia2013_COI	KWIB50	1	BC63750_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	CTTAGTGGCATTAATTCGDAATCC	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63750_Sspa_Oph2007_COI	KWIB50	1	BC63750_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	GAAGGCCTTCCGATGATC	115.76393
BC63750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20664	WGS84	BC63750	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63750_Upin_Bot2011_COI	KWIB50	1	BC63750_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63750	Sample	BC63750	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB50	COI	45	BC63750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB50	CTTTAATTACAGCGTTTTTATTGTTGT	115.76393
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63751_Cjap_Sim2018_COI	KWIB45	1	BC63751_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76082
BC63751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20989	WGS84	BC63751	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63751_Mgig_Bot2012_COI	KWIB45	1	BC63751_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63751	Sample	BC63751	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB45	COI	45	BC63751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB45	TCTTATTCGTTGGAGACTTTATAACCCT	115.76082
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63752_Cmae_Bot2011_COI	KWIB40	1	BC63752_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	ATGAACAGTCTATCCTCCTTTAG	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63752_Msal_Bot2012_COI	KWIB40	1	BC63752_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	GYTAGTTCCRATGATGTTAGCTG	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63752_Pvir_Dia2013_COI	KWIB40	1	BC63752_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	CTTAGTGGCATTAATTCGDAATCC	115.7574
BC63752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23094	WGS84	BC63752	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63752_Rhar_Sim2018_COI	KWIB40	1	BC63752_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63752	Sample	BC63752	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB40	COI	45	BC63752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB40	CTGCTGCTATTGCTCATG	115.7574
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63754_Aamu_Bax2006_COI	KWIB30	1	BC63754_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	GCACAACCGGGATCTTTACTTC	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63754_Pcan_Bot2011_IGS	KWIB30	1	BC63754_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB30	IGS	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	CGTAATCCTCAGTACTGGCTA	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63754_Pper_Dia2013_COI	KWIB30	1	BC63754_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	CTTAGTGGCATTAATTCGDAATCC	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.959	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63754_Sspa_Oph2007_COI	KWIB30	1	BC63754_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	GAAGGCCTTCCGATGATC	115.75607
BC63754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23659	WGS84	BC63754	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63754_Upin_Bot2011_COI	KWIB30	1	BC63754_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63754	Sample	BC63754	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB30	COI	45	BC63754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB30	CTTTAATTACAGCGTTTTTATTGTTGT	115.75607
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63755_Cjap_Sim2018_COI	KWIB25	1	BC63755_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75317
BC63755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23658	WGS84	BC63755	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63755_Mgig_Bot2012_COI	KWIB25	1	BC63755_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63755	Sample	BC63755	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB25	COI	45	BC63755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB25	TCTTATTCGTTGGAGACTTTATAACCCT	115.75317
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63756_Cmae_Bot2011_COI	KWIB20	1	BC63756_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	ATGAACAGTCTATCCTCCTTTAG	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63756_Msal_Bot2012_COI	KWIB20	1	BC63756_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	GYTAGTTCCRATGATGTTAGCTG	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63756_Pvir_Dia2013_COI	KWIB20	1	BC63756_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	CTTAGTGGCATTAATTCGDAATCC	115.75447
BC63756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23955	WGS84	BC63756	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63756_Rhar_Sim2018_COI	KWIB20	1	BC63756_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63756	Sample	BC63756	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB20	COI	45	BC63756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB20	CTGCTGCTATTGCTCATG	115.75447
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63758_Aamu_Bax2006_COI	KWIB10	1	BC63758_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	GCACAACCGGGATCTTTACTTC	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63758_Pcan_Bot2011_IGS	KWIB10	1	BC63758_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB10	IGS	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	CGTAATCCTCAGTACTGGCTA	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63758_Pper_Dia2013_COI	KWIB10	1	BC63758_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	CTTAGTGGCATTAATTCGDAATCC	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63758_Sspa_Oph2007_COI	KWIB10	1	BC63758_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	GAAGGCCTTCCGATGATC	115.74383
BC63758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2519	WGS84	BC63758	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63758_Upin_Bot2011_COI	KWIB10	1	BC63758_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63758	Sample	BC63758	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB10	COI	45	BC63758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB10	CTTTAATTACAGCGTTTTTATTGTTGT	115.74383
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63759_Cjap_Sim2018_COI	KWIB5	1	BC63759_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74466
BC63759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25546	WGS84	BC63759	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63759_Mgig_Bot2012_COI	KWIB5	1	BC63759_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63759	Sample	BC63759	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB5	COI	45	BC63759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB5	TCTTATTCGTTGGAGACTTTATAACCCT	115.74466
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63761_Aamu_Bax2006_COI	KWIB58	1	BC63761_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	GCACAACCGGGATCTTTACTTC	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63761_Pcan_Bot2011_IGS	KWIB58	1	BC63761_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB58	IGS	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	CGTAATCCTCAGTACTGGCTA	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63761_Pper_Dia2013_COI	KWIB58	1	BC63761_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	CTTAGTGGCATTAATTCGDAATCC	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63761_Sspa_Oph2007_COI	KWIB58	1	BC63761_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	GAAGGCCTTCCGATGATC	115.76952
BC63761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.18819	WGS84	BC63761	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63761_Upin_Bot2011_COI	KWIB58	1	BC63761_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63761	Sample	BC63761	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB58	COI	45	BC63761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB58	CTTTAATTACAGCGTTTTTATTGTTGT	115.76952
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63762_Cjap_Sim2018_COI	KWIB57	1	BC63762_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7729
BC63762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19084	WGS84	BC63762	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63762_Mgig_Bot2012_COI	KWIB57	1	BC63762_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63762	Sample	BC63762	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB57	COI	45	BC63762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB57	TCTTATTCGTTGGAGACTTTATAACCCT	115.7729
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63763_Cmae_Bot2011_COI	KWIB56	1	BC63763_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	ATGAACAGTCTATCCTCCTTTAG	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63763_Msal_Bot2012_COI	KWIB56	1	BC63763_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	GYTAGTTCCRATGATGTTAGCTG	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63763_Pvir_Dia2013_COI	KWIB56	1	BC63763_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	CTTAGTGGCATTAATTCGDAATCC	115.7711
BC63763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19125	WGS84	BC63763	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63763_Rhar_Sim2018_COI	KWIB56	1	BC63763_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63763	Sample	BC63763	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB56	COI	45	BC63763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB56	CTGCTGCTATTGCTCATG	115.7711
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63765_Aamu_Bax2006_COI	KWIB53	1	BC63765_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	GCACAACCGGGATCTTTACTTC	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63765_Pcan_Bot2011_IGS	KWIB53	1	BC63765_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB53	IGS	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	CGTAATCCTCAGTACTGGCTA	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63765_Pper_Dia2013_COI	KWIB53	1	BC63765_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	CTTAGTGGCATTAATTCGDAATCC	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63765_Sspa_Oph2007_COI	KWIB53	1	BC63765_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	GAAGGCCTTCCGATGATC	115.77138
BC63765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19141	WGS84	BC63765	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63765_Upin_Bot2011_COI	KWIB53	1	BC63765_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63765	Sample	BC63765	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB53	COI	45	BC63765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB53	CTTTAATTACAGCGTTTTTATTGTTGT	115.77138
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63766_Cjap_Sim2018_COI	KWIB52	1	BC63766_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76958
BC63766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.19132	WGS84	BC63766	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63766_Mgig_Bot2012_COI	KWIB52	1	BC63766_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63766	Sample	BC63766	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB52	COI	45	BC63766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB52	TCTTATTCGTTGGAGACTTTATAACCCT	115.76958
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63767_Cmae_Bot2011_COI	KWIB51	1	BC63767_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	ATGAACAGTCTATCCTCCTTTAG	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63767_Msal_Bot2012_COI	KWIB51	1	BC63767_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	GYTAGTTCCRATGATGTTAGCTG	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63767_Pvir_Dia2013_COI	KWIB51	1	BC63767_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	CTTAGTGGCATTAATTCGDAATCC	115.76639
BC63767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.20739	WGS84	BC63767	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63767_Rhar_Sim2018_COI	KWIB51	1	BC63767_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63767	Sample	BC63767	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB51	COI	45	BC63767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB51	CTGCTGCTATTGCTCATG	115.76639
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63769_Aamu_Bax2006_COI	KWIB48	1	BC63769_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	GCACAACCGGGATCTTTACTTC	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63769_Pcan_Bot2011_IGS	KWIB48	1	BC63769_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB48	IGS	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	CGTAATCCTCAGTACTGGCTA	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63769_Pper_Dia2013_COI	KWIB48	1	BC63769_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	CTTAGTGGCATTAATTCGDAATCC	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63769_Sspa_Oph2007_COI	KWIB48	1	BC63769_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	GAAGGCCTTCCGATGATC	115.76106
BC63769	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2086	WGS84	BC63769	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63769_Upin_Bot2011_COI	KWIB48	1	BC63769_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63769	Sample	BC63769	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB48	COI	45	BC63769	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB48	CTTTAATTACAGCGTTTTTATTGTTGT	115.76106
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63770_Cmae_Bot2011_COI	KWIB47	1	BC63770_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	ATGAACAGTCTATCCTCCTTTAG	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63770_Msal_Bot2012_COI	KWIB47	1	BC63770_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	GYTAGTTCCRATGATGTTAGCTG	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63770_Pvir_Dia2013_COI	KWIB47	1	BC63770_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	CTTAGTGGCATTAATTCGDAATCC	115.76457
BC63770	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21047	WGS84	BC63770	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63770_Rhar_Sim2018_COI	KWIB47	1	BC63770_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63770	Sample	BC63770	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB47	COI	45	BC63770	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB47	CTGCTGCTATTGCTCATG	115.76457
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63772_Aamu_Bax2006_COI	KWIB44	1	BC63772_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	GCACAACCGGGATCTTTACTTC	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63772_Pcan_Bot2011_IGS	KWIB44	1	BC63772_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB44	IGS	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	CGTAATCCTCAGTACTGGCTA	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63772_Pper_Dia2013_COI	KWIB44	1	BC63772_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	CTTAGTGGCATTAATTCGDAATCC	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63772_Sspa_Oph2007_COI	KWIB44	1	BC63772_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	GAAGGCCTTCCGATGATC	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63772	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63772_Upin_Bot2011_COI	KWIB44	1	BC63772_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	CTTTAATTACAGCGTTTTTATTGTTGT	115.76168
BC63772	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21242	WGS84	BC63772	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63772_Vgib_Wil2025_COI	KWIB44	1	BC63772_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63772	Sample	BC63772	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB44	COI	45	BC63772	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB44	CCCCTCTTTACAAGTTCTTTAGGGT	115.76168
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.21242	WGS84	BC63773	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	31.652	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63773_Asen_Wil2025_COI	KWIB43	1	BC63773_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Present	Sample	Asen_Wil2025_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	GTTGTTGTTACAACACATGCTCTTTTG	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63773_Cjap_Sim2018_COI	KWIB43	1	BC63773_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75911
BC63773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.21242	WGS84	BC63773	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63773_Mgig_Bot2012_COI	KWIB43	1	BC63773_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63773	Sample	BC63773	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB43	COI	45	BC63773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB43	TCTTATTCGTTGGAGACTTTATAACCCT	115.75911
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63774_Cmae_Bot2011_COI	KWIB42	1	BC63774_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	ATGAACAGTCTATCCTCCTTTAG	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63774_Msal_Bot2012_COI	KWIB42	1	BC63774_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	GYTAGTTCCRATGATGTTAGCTG	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63774_Pvir_Dia2013_COI	KWIB42	1	BC63774_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	CTTAGTGGCATTAATTCGDAATCC	115.75677
BC63774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22943	WGS84	BC63774	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63774_Rhar_Sim2018_COI	KWIB42	1	BC63774_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63774	Sample	BC63774	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB42	COI	45	BC63774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB42	CTGCTGCTATTGCTCATG	115.75677
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63776_Aamu_Bax2006_COI	KWIB39	1	BC63776_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	GCACAACCGGGATCTTTACTTC	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63776_Pcan_Bot2011_IGS	KWIB39	1	BC63776_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB39	IGS	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	CGTAATCCTCAGTACTGGCTA	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63776_Pper_Dia2013_COI	KWIB39	1	BC63776_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	CTTAGTGGCATTAATTCGDAATCC	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63776_Sspa_Oph2007_COI	KWIB39	1	BC63776_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	GAAGGCCTTCCGATGATC	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22993	WGS84	BC63776	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63776_Upin_Bot2011_COI	KWIB39	1	BC63776_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	CTTTAATTACAGCGTTTTTATTGTTGT	115.75608
BC63776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22993	WGS84	BC63776	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63776_Vgib_Wil2025_COI	KWIB39	1	BC63776_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63776	Sample	BC63776	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB39	COI	45	BC63776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB39	CCCCTCTTTACAAGTTCTTTAGGGT	115.75608
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.22969	WGS84	BC63777	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63777_Asen_Wil2025_COI	KWIB38	1	BC63777_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	GTTGTTGTTACAACACATGCTCTTTTG	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63777_Cjap_Sim2018_COI	KWIB38	1	BC63777_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75292
BC63777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.22969	WGS84	BC63777	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63777_Mgig_Bot2012_COI	KWIB38	1	BC63777_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63777	Sample	BC63777	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB38	COI	45	BC63777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB38	TCTTATTCGTTGGAGACTTTATAACCCT	115.75292
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63778_Cmae_Bot2011_COI	KWIB37	1	BC63778_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	ATGAACAGTCTATCCTCCTTTAG	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63778_Msal_Bot2012_COI	KWIB37	1	BC63778_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	GYTAGTTCCRATGATGTTAGCTG	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63778_Pvir_Dia2013_COI	KWIB37	1	BC63778_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	CTTAGTGGCATTAATTCGDAATCC	115.7565
BC63778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23223	WGS84	BC63778	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63778_Rhar_Sim2018_COI	KWIB37	1	BC63778_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63778	Sample	BC63778	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB37	COI	45	BC63778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB37	CTGCTGCTATTGCTCATG	115.7565
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23233	WGS84	BC63780	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63780_Asen_Wil2025_COI	KWIB34	1	BC63780_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	GTTGTTGTTACAACACATGCTCTTTTG	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63780_Cjap_Sim2018_COI	KWIB34	1	BC63780_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75612
BC63780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23233	WGS84	BC63780	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63780_Mgig_Bot2012_COI	KWIB34	1	BC63780_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63780	Sample	BC63780	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB34	COI	45	BC63780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB34	TCTTATTCGTTGGAGACTTTATAACCCT	115.75612
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63781_Cmae_Bot2011_COI	KWIB33	1	BC63781_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	ATGAACAGTCTATCCTCCTTTAG	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63781_Msal_Bot2012_COI	KWIB33	1	BC63781_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	GYTAGTTCCRATGATGTTAGCTG	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63781_Pvir_Dia2013_COI	KWIB33	1	BC63781_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	CTTAGTGGCATTAATTCGDAATCC	115.75438
BC63781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23243	WGS84	BC63781	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63781_Rhar_Sim2018_COI	KWIB33	1	BC63781_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63781	Sample	BC63781	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB33	COI	45	BC63781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB33	CTGCTGCTATTGCTCATG	115.75438
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63783_Aamu_Bax2006_COI	KWIB31	1	BC63783_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	GCACAACCGGGATCTTTACTTC	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63783_Pcan_Bot2011_IGS	KWIB31	1	BC63783_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB31	IGS	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	CGTAATCCTCAGTACTGGCTA	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63783_Pper_Dia2013_COI	KWIB31	1	BC63783_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	CTTAGTGGCATTAATTCGDAATCC	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63783_Sspa_Oph2007_COI	KWIB31	1	BC63783_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	GAAGGCCTTCCGATGATC	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23656	WGS84	BC63783	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63783_Upin_Bot2011_COI	KWIB31	1	BC63783_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	CTTTAATTACAGCGTTTTTATTGTTGT	115.75781
BC63783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23656	WGS84	BC63783	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63783_Vgib_Wil2025_COI	KWIB31	1	BC63783_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63783	Sample	BC63783	targeted	Absent	Sample	Vgib_Wil2025_COI	KWIB31	COI	45	BC63783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB31	CCCCTCTTTACAAGTTCTTTAGGGT	115.75781
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-32.23605	WGS84	BC63784	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63784_Asen_Wil2025_COI	KWIB29	1	BC63784_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Asen_Wil2025_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	GTTGTTGTTACAACACATGCTCTTTTG	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63784_Cjap_Sim2018_COI	KWIB29	1	BC63784_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75441
BC63784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23605	WGS84	BC63784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63784_Mgig_Bot2012_COI	KWIB29	1	BC63784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63784	Sample	BC63784	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB29	COI	45	BC63784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB29	TCTTATTCGTTGGAGACTTTATAACCCT	115.75441
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63785_Cmae_Bot2011_COI	KWIB28	1	BC63785_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	ATGAACAGTCTATCCTCCTTTAG	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63785_Msal_Bot2012_COI	KWIB28	1	BC63785_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	GYTAGTTCCRATGATGTTAGCTG	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63785_Pvir_Dia2013_COI	KWIB28	1	BC63785_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	CTTAGTGGCATTAATTCGDAATCC	115.75654
BC63785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2377	WGS84	BC63785	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63785_Rhar_Sim2018_COI	KWIB28	1	BC63785_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63785	Sample	BC63785	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB28	COI	45	BC63785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB28	CTGCTGCTATTGCTCATG	115.75654
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63787_Aamu_Bax2006_COI	KWIB26	1	BC63787_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	GCACAACCGGGATCTTTACTTC	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63787_Pcan_Bot2011_IGS	KWIB26	1	BC63787_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB26	IGS	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	CGTAATCCTCAGTACTGGCTA	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63787_Pper_Dia2013_COI	KWIB26	1	BC63787_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	CTTAGTGGCATTAATTCGDAATCC	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63787_Sspa_Oph2007_COI	KWIB26	1	BC63787_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	GAAGGCCTTCCGATGATC	115.75575
BC63787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23738	WGS84	BC63787	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63787_Upin_Bot2011_COI	KWIB26	1	BC63787_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63787	Sample	BC63787	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB26	COI	45	BC63787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB26	CTTTAATTACAGCGTTTTTATTGTTGT	115.75575
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63788_Cjap_Sim2018_COI	KWIB24	1	BC63788_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75724
BC63788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23881	WGS84	BC63788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63788_Mgig_Bot2012_COI	KWIB24	1	BC63788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63788	Sample	BC63788	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB24	COI	45	BC63788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB24	TCTTATTCGTTGGAGACTTTATAACCCT	115.75724
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63789_Cmae_Bot2011_COI	KWIB23	1	BC63789_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	ATGAACAGTCTATCCTCCTTTAG	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63789_Msal_Bot2012_COI	KWIB23	1	BC63789_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	GYTAGTTCCRATGATGTTAGCTG	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63789_Pvir_Dia2013_COI	KWIB23	1	BC63789_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	CTTAGTGGCATTAATTCGDAATCC	115.75448
BC63789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2382	WGS84	BC63789	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63789_Rhar_Sim2018_COI	KWIB23	1	BC63789_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63789	Sample	BC63789	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB23	COI	45	BC63789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB23	CTGCTGCTATTGCTCATG	115.75448
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63790_Aamu_Bax2006_COI	KWIB22	1	BC63790_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	GCACAACCGGGATCTTTACTTC	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63790_Pcan_Bot2011_IGS	KWIB22	1	BC63790_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB22	IGS	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	CGTAATCCTCAGTACTGGCTA	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63790_Pper_Dia2013_COI	KWIB22	1	BC63790_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	CTTAGTGGCATTAATTCGDAATCC	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63790_Sspa_Oph2007_COI	KWIB22	1	BC63790_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	GAAGGCCTTCCGATGATC	115.75808
BC63790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24026	WGS84	BC63790	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63790_Upin_Bot2011_COI	KWIB22	1	BC63790_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63790	Sample	BC63790	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB22	COI	45	BC63790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB22	CTTTAATTACAGCGTTTTTATTGTTGT	115.75808
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63791_Cjap_Sim2018_COI	KWIB21	1	BC63791_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75679
BC63791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23994	WGS84	BC63791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63791_Mgig_Bot2012_COI	KWIB21	1	BC63791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63791	Sample	BC63791	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB21	COI	45	BC63791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB21	TCTTATTCGTTGGAGACTTTATAACCCT	115.75679
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63792_Cmae_Bot2011_COI	KWIB19	1	BC63792_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	ATGAACAGTCTATCCTCCTTTAG	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63792_Msal_Bot2012_COI	KWIB19	1	BC63792_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	GYTAGTTCCRATGATGTTAGCTG	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63792_Pvir_Dia2013_COI	KWIB19	1	BC63792_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	CTTAGTGGCATTAATTCGDAATCC	115.75341
BC63792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.23976	WGS84	BC63792	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63792_Rhar_Sim2018_COI	KWIB19	1	BC63792_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63792	Sample	BC63792	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB19	COI	45	BC63792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB19	CTGCTGCTATTGCTCATG	115.75341
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63794_Aamu_Bax2006_COI	KWIB17	1	BC63794_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	GCACAACCGGGATCTTTACTTC	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63794_Pcan_Bot2011_IGS	KWIB17	1	BC63794_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB17	IGS	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	CGTAATCCTCAGTACTGGCTA	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63794_Pper_Dia2013_COI	KWIB17	1	BC63794_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	CTTAGTGGCATTAATTCGDAATCC	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63794_Sspa_Oph2007_COI	KWIB17	1	BC63794_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	GAAGGCCTTCCGATGATC	115.75376
BC63794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24202	WGS84	BC63794	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63794_Upin_Bot2011_COI	KWIB17	1	BC63794_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63794	Sample	BC63794	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB17	COI	45	BC63794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB17	CTTTAATTACAGCGTTTTTATTGTTGT	115.75376
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63795_Cjap_Sim2018_COI	KWIB16	1	BC63795_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75743
BC63795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24364	WGS84	BC63795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63795_Mgig_Bot2012_COI	KWIB16	1	BC63795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63795	Sample	BC63795	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB16	COI	45	BC63795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB16	TCTTATTCGTTGGAGACTTTATAACCCT	115.75743
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63796_Cmae_Bot2011_COI	KWIB14	1	BC63796_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	ATGAACAGTCTATCCTCCTTTAG	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63796_Msal_Bot2012_COI	KWIB14	1	BC63796_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	GYTAGTTCCRATGATGTTAGCTG	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63796_Pvir_Dia2013_COI	KWIB14	1	BC63796_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	CTTAGTGGCATTAATTCGDAATCC	115.7535
BC63796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24277	WGS84	BC63796	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63796_Rhar_Sim2018_COI	KWIB14	1	BC63796_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63796	Sample	BC63796	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB14	COI	45	BC63796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB14	CTGCTGCTATTGCTCATG	115.7535
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63798_Aamu_Bax2006_COI	KWIB12	1	BC63798_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	GCACAACCGGGATCTTTACTTC	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63798_Pcan_Bot2011_IGS	KWIB12	1	BC63798_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB12	IGS	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	CGTAATCCTCAGTACTGGCTA	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63798_Pper_Dia2013_COI	KWIB12	1	BC63798_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	CTTAGTGGCATTAATTCGDAATCC	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63798_Sspa_Oph2007_COI	KWIB12	1	BC63798_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	GAAGGCCTTCCGATGATC	115.75291
BC63798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.24371	WGS84	BC63798	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63798_Upin_Bot2011_COI	KWIB12	1	BC63798_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63798	Sample	BC63798	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB12	COI	45	BC63798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB12	CTTTAATTACAGCGTTTTTATTGTTGT	115.75291
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63799_Cjap_Sim2018_COI	KWIB11	1	BC63799_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74522
BC63799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25199	WGS84	BC63799	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63799_Mgig_Bot2012_COI	KWIB11	1	BC63799_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63799	Sample	BC63799	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB11	COI	45	BC63799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB11	TCTTATTCGTTGGAGACTTTATAACCCT	115.74522
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63800_Cmae_Bot2011_COI	KWIB9	1	BC63800_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	ATGAACAGTCTATCCTCCTTTAG	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63800_Msal_Bot2012_COI	KWIB9	1	BC63800_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	GYTAGTTCCRATGATGTTAGCTG	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63800_Pvir_Dia2013_COI	KWIB9	1	BC63800_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	CTTAGTGGCATTAATTCGDAATCC	115.74274
BC63800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25183	WGS84	BC63800	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63800_Rhar_Sim2018_COI	KWIB9	1	BC63800_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63800	Sample	BC63800	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB9	COI	45	BC63800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB9	CTGCTGCTATTGCTCATG	115.74274
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63802_Aamu_Bax2006_COI	KWIB7	1	BC63802_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	GCACAACCGGGATCTTTACTTC	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63802_Pcan_Bot2011_IGS	KWIB7	1	BC63802_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB7	IGS	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	CGTAATCCTCAGTACTGGCTA	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63802_Pper_Dia2013_COI	KWIB7	1	BC63802_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	CTTAGTGGCATTAATTCGDAATCC	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.577	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63802_Sspa_Oph2007_COI	KWIB7	1	BC63802_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Present	Sample	Sspa_Oph2007_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	GAAGGCCTTCCGATGATC	115.74276
BC63802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25302	WGS84	BC63802	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63802_Upin_Bot2011_COI	KWIB7	1	BC63802_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63802	Sample	BC63802	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB7	COI	45	BC63802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB7	CTTTAATTACAGCGTTTTTATTGTTGT	115.74276
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63803_Cjap_Sim2018_COI	KWIB6	1	BC63803_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74078
BC63803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25287	WGS84	BC63803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63803_Mgig_Bot2012_COI	KWIB6	1	BC63803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63803	Sample	BC63803	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB6	COI	45	BC63803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB6	TCTTATTCGTTGGAGACTTTATAACCCT	115.74078
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63804_Cmae_Bot2011_COI	KWIB4	1	BC63804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	ATGAACAGTCTATCCTCCTTTAG	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63804_Msal_Bot2012_COI	KWIB4	1	BC63804_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Msal_Bot2012_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	GYTAGTTCCRATGATGTTAGCTG	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63804_Pvir_Dia2013_COI	KWIB4	1	BC63804_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	CTTAGTGGCATTAATTCGDAATCC	115.74288
BC63804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25516	WGS84	BC63804	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63804_Rhar_Sim2018_COI	KWIB4	1	BC63804_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63804	Sample	BC63804	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIB4	COI	45	BC63804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB4	CTGCTGCTATTGCTCATG	115.74288
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63806_Aamu_Bax2006_COI	KWIB2	1	BC63806_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	GCACAACCGGGATCTTTACTTC	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63806_Pcan_Bot2011_IGS	KWIB2	1	BC63806_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIB2	IGS	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	CGTAATCCTCAGTACTGGCTA	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63806_Pper_Dia2013_COI	KWIB2	1	BC63806_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Pper_Dia2013_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	CTTAGTGGCATTAATTCGDAATCC	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63806_Sspa_Oph2007_COI	KWIB2	1	BC63806_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	GAAGGCCTTCCGATGATC	115.74169
BC63806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.2558	WGS84	BC63806	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63806_Upin_Bot2011_COI	KWIB2	1	BC63806_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63806	Sample	BC63806	targeted	Absent	Sample	Upin_Bot2011_COI	KWIB2	COI	45	BC63806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB2	CTTTAATTACAGCGTTTTTATTGTTGT	115.74169
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63807_Cjap_Sim2018_COI	KWIB1	1	BC63807_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74057
BC63807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13075	-32.25607	WGS84	BC63807	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Autumn	5/12/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63807_Mgig_Bot2012_COI	KWIB1	1	BC63807_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63807	Sample	BC63807	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIB1	COI	45	BC63807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIB1	TCTTATTCGTTGGAGACTTTATAACCCT	115.74057
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63808_Cmae_Bot2011_COI	BUNB20	1	BC63808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	ATGAACAGTCTATCCTCCTTTAG	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63808_Msal_Bot2012_COI	BUNB20	1	BC63808_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	GYTAGTTCCRATGATGTTAGCTG	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63808_Pvir_Dia2013_COI	BUNB20	1	BC63808_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	CTTAGTGGCATTAATTCGDAATCC	115.64503
BC63808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30696	WGS84	BC63808	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63808_Rhar_Sim2018_COI	BUNB20	1	BC63808_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63808	Sample	BC63808	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB20	COI	45	BC63808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB20	CTGCTGCTATTGCTCATG	115.64503
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63810_Cjap_Sim2018_COI	BUNB10	1	BC63810_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66527
BC63810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32313	WGS84	BC63810	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63810_Mgig_Bot2012_COI	BUNB10	1	BC63810_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63810	Sample	BC63810	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB10	COI	45	BC63810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB10	TCTTATTCGTTGGAGACTTTATAACCCT	115.66527
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63811_Cmae_Bot2011_COI	BUNB5	1	BC63811_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	ATGAACAGTCTATCCTCCTTTAG	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63811_Msal_Bot2012_COI	BUNB5	1	BC63811_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	GYTAGTTCCRATGATGTTAGCTG	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63811_Pvir_Dia2013_COI	BUNB5	1	BC63811_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	CTTAGTGGCATTAATTCGDAATCC	115.66712
BC63811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32473	WGS84	BC63811	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63811_Rhar_Sim2018_COI	BUNB5	1	BC63811_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63811	Sample	BC63811	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB5	COI	45	BC63811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB5	CTGCTGCTATTGCTCATG	115.66712
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63813_Aamu_Bax2006_COI	BUNB22	1	BC63813_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	GCACAACCGGGATCTTTACTTC	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63813_Pcan_Bot2011_IGS	BUNB22	1	BC63813_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB22	IGS	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	CGTAATCCTCAGTACTGGCTA	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63813_Pper_Dia2013_COI	BUNB22	1	BC63813_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	CTTAGTGGCATTAATTCGDAATCC	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63813_Sspa_Oph2007_COI	BUNB22	1	BC63813_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	GAAGGCCTTCCGATGATC	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30647	WGS84	BC63813	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63813_Upin_Bot2011_COI	BUNB22	1	BC63813_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	CTTTAATTACAGCGTTTTTATTGTTGT	115.64537
BC63813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.30647	WGS84	BC63813	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63813_Vgib_Wil2025_COI	BUNB22	1	BC63813_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63813	Sample	BC63813	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB22	COI	45	BC63813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB22	CCCCTCTTTACAAGTTCTTTAGGGT	115.64537
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-33.30753	WGS84	BC63814	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63814_Asen_Wil2025_COI	BUNB21	1	BC63814_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	GTTGTTGTTACAACACATGCTCTTTTG	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63814_Cjap_Sim2018_COI	BUNB21	1	BC63814_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64716
BC63814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30753	WGS84	BC63814	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63814_Mgig_Bot2012_COI	BUNB21	1	BC63814_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63814	Sample	BC63814	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB21	COI	45	BC63814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB21	TCTTATTCGTTGGAGACTTTATAACCCT	115.64716
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63815_Cmae_Bot2011_COI	BUNB19	1	BC63815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	ATGAACAGTCTATCCTCCTTTAG	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63815_Msal_Bot2012_COI	BUNB19	1	BC63815_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	GYTAGTTCCRATGATGTTAGCTG	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63815_Pvir_Dia2013_COI	BUNB19	1	BC63815_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	CTTAGTGGCATTAATTCGDAATCC	115.64399
BC63815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.30822	WGS84	BC63815	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63815_Rhar_Sim2018_COI	BUNB19	1	BC63815_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63815	Sample	BC63815	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB19	COI	45	BC63815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB19	CTGCTGCTATTGCTCATG	115.64399
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63817_Aamu_Bax2006_COI	BUNB17	1	BC63817_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	GCACAACCGGGATCTTTACTTC	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63817_Pcan_Bot2011_IGS	BUNB17	1	BC63817_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB17	IGS	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	CGTAATCCTCAGTACTGGCTA	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63817_Pper_Dia2013_COI	BUNB17	1	BC63817_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	CTTAGTGGCATTAATTCGDAATCC	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63817_Sspa_Oph2007_COI	BUNB17	1	BC63817_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	GAAGGCCTTCCGATGATC	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63817_Upin_Bot2011_COI	BUNB17	1	BC63817_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	CTTTAATTACAGCGTTTTTATTGTTGT	115.66296
BC63817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32099	WGS84	BC63817	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63817_Vgib_Wil2025_COI	BUNB17	1	BC63817_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63817	Sample	BC63817	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB17	COI	45	BC63817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB17	CCCCTCTTTACAAGTTCTTTAGGGT	115.66296
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63818_Asen_Wil2025_COI	BUNB16	1	BC63818_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	GTTGTTGTTACAACACATGCTCTTTTG	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63818_Cjap_Sim2018_COI	BUNB16	1	BC63818_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.6614
BC63818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.3204	WGS84	BC63818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63818_Mgig_Bot2012_COI	BUNB16	1	BC63818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63818	Sample	BC63818	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB16	COI	45	BC63818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB16	TCTTATTCGTTGGAGACTTTATAACCCT	115.6614
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63819_Cmae_Bot2011_COI	BUNB14	1	BC63819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	ATGAACAGTCTATCCTCCTTTAG	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63819_Msal_Bot2012_COI	BUNB14	1	BC63819_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	GYTAGTTCCRATGATGTTAGCTG	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63819_Pvir_Dia2013_COI	BUNB14	1	BC63819_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	CTTAGTGGCATTAATTCGDAATCC	115.6627
BC63819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32125	WGS84	BC63819	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63819_Rhar_Sim2018_COI	BUNB14	1	BC63819_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63819	Sample	BC63819	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB14	COI	45	BC63819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB14	CTGCTGCTATTGCTCATG	115.6627
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63820_Aamu_Bax2006_COI	BUNB13	1	BC63820_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	GCACAACCGGGATCTTTACTTC	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63820_Pcan_Bot2011_IGS	BUNB13	1	BC63820_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB13	IGS	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	CGTAATCCTCAGTACTGGCTA	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63820_Pper_Dia2013_COI	BUNB13	1	BC63820_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	CTTAGTGGCATTAATTCGDAATCC	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63820_Sspa_Oph2007_COI	BUNB13	1	BC63820_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	GAAGGCCTTCCGATGATC	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63820_Upin_Bot2011_COI	BUNB13	1	BC63820_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	CTTTAATTACAGCGTTTTTATTGTTGT	115.66204
BC63820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32167	WGS84	BC63820	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63820_Vgib_Wil2025_COI	BUNB13	1	BC63820_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63820	Sample	BC63820	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB13	COI	45	BC63820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB13	CCCCTCTTTACAAGTTCTTTAGGGT	115.66204
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63821_Asen_Wil2025_COI	BUNB12	1	BC63821_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	GTTGTTGTTACAACACATGCTCTTTTG	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63821_Cjap_Sim2018_COI	BUNB12	1	BC63821_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66029
BC63821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32148	WGS84	BC63821	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63821_Mgig_Bot2012_COI	BUNB12	1	BC63821_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63821	Sample	BC63821	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB12	COI	45	BC63821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB12	TCTTATTCGTTGGAGACTTTATAACCCT	115.66029
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63822_Cmae_Bot2011_COI	BUNB11	1	BC63822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	ATGAACAGTCTATCCTCCTTTAG	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63822_Msal_Bot2012_COI	BUNB11	1	BC63822_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	GYTAGTTCCRATGATGTTAGCTG	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63822_Pvir_Dia2013_COI	BUNB11	1	BC63822_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	CTTAGTGGCATTAATTCGDAATCC	115.6586
BC63822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32095	WGS84	BC63822	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63822_Rhar_Sim2018_COI	BUNB11	1	BC63822_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63822	Sample	BC63822	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB11	COI	45	BC63822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB11	CTGCTGCTATTGCTCATG	115.6586
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63824_Aamu_Bax2006_COI	BUNB8	1	BC63824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	GCACAACCGGGATCTTTACTTC	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63824_Pcan_Bot2011_IGS	BUNB8	1	BC63824_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB8	IGS	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	CGTAATCCTCAGTACTGGCTA	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63824_Pper_Dia2013_COI	BUNB8	1	BC63824_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	CTTAGTGGCATTAATTCGDAATCC	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63824_Sspa_Oph2007_COI	BUNB8	1	BC63824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	GAAGGCCTTCCGATGATC	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63824_Upin_Bot2011_COI	BUNB8	1	BC63824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	CTTTAATTACAGCGTTTTTATTGTTGT	115.66286
BC63824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32295	WGS84	BC63824	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63824_Vgib_Wil2025_COI	BUNB8	1	BC63824_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63824	Sample	BC63824	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB8	COI	45	BC63824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB8	CCCCTCTTTACAAGTTCTTTAGGGT	115.66286
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63825_Asen_Wil2025_COI	BUNB7	1	BC63825_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	GTTGTTGTTACAACACATGCTCTTTTG	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63825_Cjap_Sim2018_COI	BUNB7	1	BC63825_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66108
BC63825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32256	WGS84	BC63825	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63825_Mgig_Bot2012_COI	BUNB7	1	BC63825_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63825	Sample	BC63825	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB7	COI	45	BC63825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB7	TCTTATTCGTTGGAGACTTTATAACCCT	115.66108
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63826_Cmae_Bot2011_COI	BUNB6	1	BC63826_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	ATGAACAGTCTATCCTCCTTTAG	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63826_Msal_Bot2012_COI	BUNB6	1	BC63826_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Msal_Bot2012_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	GYTAGTTCCRATGATGTTAGCTG	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63826_Pvir_Dia2013_COI	BUNB6	1	BC63826_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	CTTAGTGGCATTAATTCGDAATCC	115.66757
BC63826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32494	WGS84	BC63826	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63826_Rhar_Sim2018_COI	BUNB6	1	BC63826_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63826	Sample	BC63826	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNB6	COI	45	BC63826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB6	CTGCTGCTATTGCTCATG	115.66757
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63828_Aamu_Bax2006_COI	BUNB3	1	BC63828_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	GCACAACCGGGATCTTTACTTC	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63828_Pcan_Bot2011_IGS	BUNB3	1	BC63828_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNB3	IGS	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	CGTAATCCTCAGTACTGGCTA	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63828_Pper_Dia2013_COI	BUNB3	1	BC63828_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Pper_Dia2013_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	CTTAGTGGCATTAATTCGDAATCC	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63828_Sspa_Oph2007_COI	BUNB3	1	BC63828_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	GAAGGCCTTCCGATGATC	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63828_Upin_Bot2011_COI	BUNB3	1	BC63828_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Upin_Bot2011_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	CTTTAATTACAGCGTTTTTATTGTTGT	115.66363
BC63828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32413	WGS84	BC63828	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63828_Vgib_Wil2025_COI	BUNB3	1	BC63828_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63828	Sample	BC63828	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNB3	COI	45	BC63828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB3	CCCCTCTTTACAAGTTCTTTAGGGT	115.66363
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63829_Asen_Wil2025_COI	BUNB2	1	BC63829_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Asen_Wil2025_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	GTTGTTGTTACAACACATGCTCTTTTG	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63829_Cjap_Sim2018_COI	BUNB2	1	BC63829_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66723
BC63829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-33.32558	WGS84	BC63829	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Autumn	14/05/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63829_Mgig_Bot2012_COI	BUNB2	1	BC63829_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63829	Sample	BC63829	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNB2	COI	45	BC63829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNB2	TCTTATTCGTTGGAGACTTTATAACCCT	115.66723
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63831_Aamu_Bax2006_COI	GERB15	1	BC63831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	GCACAACCGGGATCTTTACTTC	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63831_Pcan_Bot2011_IGS	GERB15	1	BC63831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB15	IGS	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	CGTAATCCTCAGTACTGGCTA	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63831_Pper_Dia2013_COI	GERB15	1	BC63831_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Pper_Dia2013_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	CTTAGTGGCATTAATTCGDAATCC	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63831_Sspa_Oph2007_COI	GERB15	1	BC63831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	GAAGGCCTTCCGATGATC	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63831_Upin_Bot2011_COI	GERB15	1	BC63831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Upin_Bot2011_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	CTTTAATTACAGCGTTTTTATTGTTGT	114.60004
BC63831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77206	WGS84	BC63831	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63831_Vgib_Wil2025_COI	GERB15	1	BC63831_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63831	Sample	BC63831	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB15	COI	45	BC63831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB15	CCCCTCTTTACAAGTTCTTTAGGGT	114.60004
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77465	WGS84	BC63832	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63832_Asen_Wil2025_COI	GERB10	1	BC63832_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Asen_Wil2025_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	GTTGTTGTTACAACACATGCTCTTTTG	114.60076
BC63832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77465	WGS84	BC63832	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63832_Cjap_Sim2018_COI	GERB10	1	BC63832_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63832	Sample	BC63832	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB10	COI	45	BC63832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB10	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.60076
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63833_Cmae_Bot2011_COI	GERB5	1	BC63833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	ATGAACAGTCTATCCTCCTTTAG	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63833_Msal_Bot2012_COI	GERB5	1	BC63833_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Msal_Bot2012_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	GYTAGTTCCRATGATGTTAGCTG	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63833_Pvir_Dia2013_COI	GERB5	1	BC63833_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	CTTAGTGGCATTAATTCGDAATCC	114.59844
BC63833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77534	WGS84	BC63833	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63833_Rhar_Sim2018_COI	GERB5	1	BC63833_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63833	Sample	BC63833	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB5	COI	45	BC63833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB5	CTGCTGCTATTGCTCATG	114.59844
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63835_Aamu_Bax2006_COI	GERB13	1	BC63835_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	GCACAACCGGGATCTTTACTTC	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63835_Pcan_Bot2011_IGS	GERB13	1	BC63835_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB13	IGS	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	CGTAATCCTCAGTACTGGCTA	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63835_Pper_Dia2013_COI	GERB13	1	BC63835_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Pper_Dia2013_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	CTTAGTGGCATTAATTCGDAATCC	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63835_Sspa_Oph2007_COI	GERB13	1	BC63835_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	GAAGGCCTTCCGATGATC	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77301	WGS84	BC63835	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63835_Upin_Bot2011_COI	GERB13	1	BC63835_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Upin_Bot2011_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	CTTTAATTACAGCGTTTTTATTGTTGT	114.59874
BC63835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77301	WGS84	BC63835	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63835_Vgib_Wil2025_COI	GERB13	1	BC63835_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63835	Sample	BC63835	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB13	COI	45	BC63835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB13	CCCCTCTTTACAAGTTCTTTAGGGT	114.59874
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77283	WGS84	BC63836	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63836_Asen_Wil2025_COI	GERB12	1	BC63836_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Asen_Wil2025_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	GTTGTTGTTACAACACATGCTCTTTTG	114.59728
BC63836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77283	WGS84	BC63836	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63836_Cjap_Sim2018_COI	GERB12	1	BC63836_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63836	Sample	BC63836	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB12	COI	45	BC63836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB12	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59728
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63837_Cmae_Bot2011_COI	GERB11	1	BC63837_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	ATGAACAGTCTATCCTCCTTTAG	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63837_Msal_Bot2012_COI	GERB11	1	BC63837_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Msal_Bot2012_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	GYTAGTTCCRATGATGTTAGCTG	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63837_Pvir_Dia2013_COI	GERB11	1	BC63837_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	CTTAGTGGCATTAATTCGDAATCC	114.59585
BC63837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77278	WGS84	BC63837	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63837_Rhar_Sim2018_COI	GERB11	1	BC63837_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63837	Sample	BC63837	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB11	COI	45	BC63837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB11	CTGCTGCTATTGCTCATG	114.59585
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63839_Aamu_Bax2006_COI	GERB8	1	BC63839_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	GCACAACCGGGATCTTTACTTC	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63839_Pcan_Bot2011_IGS	GERB8	1	BC63839_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB8	IGS	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	CGTAATCCTCAGTACTGGCTA	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63839_Pper_Dia2013_COI	GERB8	1	BC63839_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Pper_Dia2013_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	CTTAGTGGCATTAATTCGDAATCC	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63839_Sspa_Oph2007_COI	GERB8	1	BC63839_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	GAAGGCCTTCCGATGATC	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77384	WGS84	BC63839	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63839_Upin_Bot2011_COI	GERB8	1	BC63839_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Upin_Bot2011_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	CTTTAATTACAGCGTTTTTATTGTTGT	114.59782
BC63839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16576	-28.77384	WGS84	BC63839	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63839_Vgib_Wil2025_COI	GERB8	1	BC63839_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63839	Sample	BC63839	targeted	Absent	Sample	Vgib_Wil2025_COI	GERB8	COI	45	BC63839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB8	CCCCTCTTTACAAGTTCTTTAGGGT	114.59782
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63840_Cmae_Bot2011_COI	GERB7	1	BC63840_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	ATGAACAGTCTATCCTCCTTTAG	114.59635
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63840_Msal_Bot2012_COI	GERB7	1	BC63840_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Msal_Bot2012_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	GYTAGTTCCRATGATGTTAGCTG	114.59635
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63840_Pvir_Dia2013_COI	GERB7	1	BC63840_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	CTTAGTGGCATTAATTCGDAATCC	114.59635
BC63840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77372	WGS84	BC63840	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63840_Rhar_Sim2018_COI	GERB7	1	BC63840_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63840	Sample	BC63840	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB7	COI	45	BC63840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB7	CTGCTGCTATTGCTCATG	114.59635
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63842_Aamu_Bax2006_COI	GERB4	1	BC63842_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	GCACAACCGGGATCTTTACTTC	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63842_Pcan_Bot2011_IGS	GERB4	1	BC63842_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB4	IGS	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	CGTAATCCTCAGTACTGGCTA	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63842_Pper_Dia2013_COI	GERB4	1	BC63842_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Pper_Dia2013_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	CTTAGTGGCATTAATTCGDAATCC	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63842_Sspa_Oph2007_COI	GERB4	1	BC63842_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	GAAGGCCTTCCGATGATC	114.59693
BC63842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77489	WGS84	BC63842	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63842_Upin_Bot2011_COI	GERB4	1	BC63842_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63842	Sample	BC63842	targeted	Absent	Sample	Upin_Bot2011_COI	GERB4	COI	45	BC63842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB4	CTTTAATTACAGCGTTTTTATTGTTGT	114.59693
BC63843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77458	WGS84	BC63843	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63843_Cjap_Sim2018_COI	GERB3	1	BC63843_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63843	Sample	BC63843	targeted	Absent	Sample	Cjap_Sim2018_COI	GERB3	COI	45	BC63843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB3	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59538
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63844_Cmae_Bot2011_COI	GERB2	1	BC63844_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Cmae_Bot2011_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	ATGAACAGTCTATCCTCCTTTAG	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63844_Msal_Bot2012_COI	GERB2	1	BC63844_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Msal_Bot2012_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	GYTAGTTCCRATGATGTTAGCTG	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63844_Pvir_Dia2013_COI	GERB2	1	BC63844_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Pvir_Dia2013_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	CTTAGTGGCATTAATTCGDAATCC	114.59591
BC63844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77588	WGS84	BC63844	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63844_Rhar_Sim2018_COI	GERB2	1	BC63844_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63844	Sample	BC63844	targeted	Absent	Sample	Rhar_Sim2018_COI	GERB2	COI	45	BC63844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB2	CTGCTGCTATTGCTCATG	114.59591
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63846_Aamu_Bax2006_COI	KWIA59	1	BC63846_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	GCACAACCGGGATCTTTACTTC	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63846_Pcan_Bot2011_IGS	KWIA59	1	BC63846_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA59	IGS	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	CGTAATCCTCAGTACTGGCTA	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63846_Pper_Dia2013_COI	KWIA59	1	BC63846_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	CTTAGTGGCATTAATTCGDAATCC	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63846_Sspa_Oph2007_COI	KWIA59	1	BC63846_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	GAAGGCCTTCCGATGATC	115.77156
BC63846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1883	WGS84	BC63846	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63846_Upin_Bot2011_COI	KWIA59	1	BC63846_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63846	Sample	BC63846	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA59	COI	45	BC63846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA59	CTTTAATTACAGCGTTTTTATTGTTGT	115.77156
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63847_Cjap_Sim2018_COI	KWIA58	1	BC63847_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76965
BC63847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.18813	WGS84	BC63847	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63847_Mgig_Bot2012_COI	KWIA58	1	BC63847_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63847	Sample	BC63847	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA58	COI	45	BC63847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA58	TCTTATTCGTTGGAGACTTTATAACCCT	115.76965
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63848_Cmae_Bot2011_COI	KWIA57	1	BC63848_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	ATGAACAGTCTATCCTCCTTTAG	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63848_Msal_Bot2012_COI	KWIA57	1	BC63848_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	GYTAGTTCCRATGATGTTAGCTG	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63848_Pvir_Dia2013_COI	KWIA57	1	BC63848_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	CTTAGTGGCATTAATTCGDAATCC	115.77329
BC63848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1904	WGS84	BC63848	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63848_Rhar_Sim2018_COI	KWIA57	1	BC63848_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63848	Sample	BC63848	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA57	COI	45	BC63848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA57	CTGCTGCTATTGCTCATG	115.77329
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63850_Cjap_Sim2018_COI	KWIA55	1	BC63850_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.77384
BC63850	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19107	WGS84	BC63850	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63850_Mgig_Bot2012_COI	KWIA55	1	BC63850_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63850	Sample	BC63850	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA55	COI	45	BC63850	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA55	TCTTATTCGTTGGAGACTTTATAACCCT	115.77384
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63851_Cmae_Bot2011_COI	KWIA54	1	BC63851_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	ATGAACAGTCTATCCTCCTTTAG	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63851_Msal_Bot2012_COI	KWIA54	1	BC63851_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	GYTAGTTCCRATGATGTTAGCTG	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63851_Pvir_Dia2013_COI	KWIA54	1	BC63851_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	CTTAGTGGCATTAATTCGDAATCC	115.7731
BC63851	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.1909	WGS84	BC63851	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63851_Rhar_Sim2018_COI	KWIA54	1	BC63851_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63851	Sample	BC63851	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA54	COI	45	BC63851	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA54	CTGCTGCTATTGCTCATG	115.7731
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63853_Aamu_Bax2006_COI	KWIA52	1	BC63853_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	GCACAACCGGGATCTTTACTTC	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63853_Pcan_Bot2011_IGS	KWIA52	1	BC63853_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA52	IGS	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	CGTAATCCTCAGTACTGGCTA	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63853_Pper_Dia2013_COI	KWIA52	1	BC63853_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	CTTAGTGGCATTAATTCGDAATCC	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63853_Sspa_Oph2007_COI	KWIA52	1	BC63853_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	GAAGGCCTTCCGATGATC	115.76936
BC63853	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.19067	WGS84	BC63853	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63853_Upin_Bot2011_COI	KWIA52	1	BC63853_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63853	Sample	BC63853	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA52	COI	45	BC63853	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA52	CTTTAATTACAGCGTTTTTATTGTTGT	115.76936
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63854_Cjap_Sim2018_COI	KWIA51	1	BC63854_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76603
BC63854	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20692	WGS84	BC63854	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63854_Mgig_Bot2012_COI	KWIA51	1	BC63854_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63854	Sample	BC63854	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA51	COI	45	BC63854	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA51	TCTTATTCGTTGGAGACTTTATAACCCT	115.76603
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63855_Cmae_Bot2011_COI	KWIA50	1	BC63855_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	ATGAACAGTCTATCCTCCTTTAG	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63855_Msal_Bot2012_COI	KWIA50	1	BC63855_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	GYTAGTTCCRATGATGTTAGCTG	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63855_Pvir_Dia2013_COI	KWIA50	1	BC63855_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	CTTAGTGGCATTAATTCGDAATCC	115.76389
BC63855	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20685	WGS84	BC63855	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63855_Rhar_Sim2018_COI	KWIA50	1	BC63855_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63855	Sample	BC63855	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA50	COI	45	BC63855	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA50	CTGCTGCTATTGCTCATG	115.76389
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63857_Aamu_Bax2006_COI	KWIA48	1	BC63857_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	GCACAACCGGGATCTTTACTTC	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63857_Pcan_Bot2011_IGS	KWIA48	1	BC63857_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA48	IGS	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	CGTAATCCTCAGTACTGGCTA	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63857_Pper_Dia2013_COI	KWIA48	1	BC63857_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	CTTAGTGGCATTAATTCGDAATCC	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63857_Sspa_Oph2007_COI	KWIA48	1	BC63857_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	GAAGGCCTTCCGATGATC	115.76244
BC63857	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20835	WGS84	BC63857	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63857_Upin_Bot2011_COI	KWIA48	1	BC63857_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63857	Sample	BC63857	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA48	COI	45	BC63857	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA48	CTTTAATTACAGCGTTTTTATTGTTGT	115.76244
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63858_Cjap_Sim2018_COI	KWIA47	1	BC63858_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76541
BC63858	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21038	WGS84	BC63858	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63858_Mgig_Bot2012_COI	KWIA47	1	BC63858_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63858	Sample	BC63858	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA47	COI	45	BC63858	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA47	TCTTATTCGTTGGAGACTTTATAACCCT	115.76541
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63859_Cmae_Bot2011_COI	KWIA46	1	BC63859_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	ATGAACAGTCTATCCTCCTTTAG	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63859_Msal_Bot2012_COI	KWIA46	1	BC63859_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	GYTAGTTCCRATGATGTTAGCTG	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63859_Pvir_Dia2013_COI	KWIA46	1	BC63859_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	CTTAGTGGCATTAATTCGDAATCC	115.7633
BC63859	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21026	WGS84	BC63859	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63859_Rhar_Sim2018_COI	KWIA46	1	BC63859_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63859	Sample	BC63859	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA46	COI	45	BC63859	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA46	CTGCTGCTATTGCTCATG	115.7633
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63860_Aamu_Bax2006_COI	KWIA45	1	BC63860_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	GCACAACCGGGATCTTTACTTC	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63860_Pcan_Bot2011_IGS	KWIA45	1	BC63860_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA45	IGS	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	CGTAATCCTCAGTACTGGCTA	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63860_Pper_Dia2013_COI	KWIA45	1	BC63860_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	CTTAGTGGCATTAATTCGDAATCC	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63860_Sspa_Oph2007_COI	KWIA45	1	BC63860_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	GAAGGCCTTCCGATGATC	115.76077
BC63860	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.20987	WGS84	BC63860	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63860_Upin_Bot2011_COI	KWIA45	1	BC63860_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63860	Sample	BC63860	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA45	COI	45	BC63860	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA45	CTTTAATTACAGCGTTTTTATTGTTGT	115.76077
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63861_Cjap_Sim2018_COI	KWIA44	1	BC63861_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.76186
BC63861	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2121	WGS84	BC63861	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63861_Mgig_Bot2012_COI	KWIA44	1	BC63861_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63861	Sample	BC63861	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA44	COI	45	BC63861	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA44	TCTTATTCGTTGGAGACTTTATAACCCT	115.76186
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63862_Cmae_Bot2011_COI	KWIA43	1	BC63862_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	ATGAACAGTCTATCCTCCTTTAG	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63862_Msal_Bot2012_COI	KWIA43	1	BC63862_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	GYTAGTTCCRATGATGTTAGCTG	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63862_Pvir_Dia2013_COI	KWIA43	1	BC63862_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	CTTAGTGGCATTAATTCGDAATCC	115.75889
BC63862	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.21126	WGS84	BC63862	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63862_Rhar_Sim2018_COI	KWIA43	1	BC63862_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63862	Sample	BC63862	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA43	COI	45	BC63862	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA43	CTGCTGCTATTGCTCATG	115.75889
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63864_Aamu_Bax2006_COI	KWIA41	1	BC63864_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	GCACAACCGGGATCTTTACTTC	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63864_Pcan_Bot2011_IGS	KWIA41	1	BC63864_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA41	IGS	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	CGTAATCCTCAGTACTGGCTA	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63864_Pper_Dia2013_COI	KWIA41	1	BC63864_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	CTTAGTGGCATTAATTCGDAATCC	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63864_Sspa_Oph2007_COI	KWIA41	1	BC63864_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	GAAGGCCTTCCGATGATC	115.75511
BC63864	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22816	WGS84	BC63864	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63864_Upin_Bot2011_COI	KWIA41	1	BC63864_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63864	Sample	BC63864	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA41	COI	45	BC63864	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA41	CTTTAATTACAGCGTTTTTATTGTTGT	115.75511
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63865_Cjap_Sim2018_COI	KWIA40	1	BC63865_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75719
BC63865	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22974	WGS84	BC63865	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63865_Mgig_Bot2012_COI	KWIA40	1	BC63865_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63865	Sample	BC63865	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA40	COI	45	BC63865	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA40	TCTTATTCGTTGGAGACTTTATAACCCT	115.75719
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63866_Cmae_Bot2011_COI	KWIA39	1	BC63866_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	ATGAACAGTCTATCCTCCTTTAG	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63866_Msal_Bot2012_COI	KWIA39	1	BC63866_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	GYTAGTTCCRATGATGTTAGCTG	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63866_Pvir_Dia2013_COI	KWIA39	1	BC63866_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	CTTAGTGGCATTAATTCGDAATCC	115.75548
BC63866	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.22959	WGS84	BC63866	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63866_Rhar_Sim2018_COI	KWIA39	1	BC63866_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63866	Sample	BC63866	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA39	COI	45	BC63866	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA39	CTGCTGCTATTGCTCATG	115.75548
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63868_Aamu_Bax2006_COI	KWIA37	1	BC63868_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	GCACAACCGGGATCTTTACTTC	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63868_Pcan_Bot2011_IGS	KWIA37	1	BC63868_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA37	IGS	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	CGTAATCCTCAGTACTGGCTA	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63868_Pper_Dia2013_COI	KWIA37	1	BC63868_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	CTTAGTGGCATTAATTCGDAATCC	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63868_Sspa_Oph2007_COI	KWIA37	1	BC63868_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	GAAGGCCTTCCGATGATC	115.75622
BC63868	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23105	WGS84	BC63868	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63868_Upin_Bot2011_COI	KWIA37	1	BC63868_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63868	Sample	BC63868	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA37	COI	45	BC63868	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA37	CTTTAATTACAGCGTTTTTATTGTTGT	115.75622
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63869_Cjap_Sim2018_COI	KWIA36	1	BC63869_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75422
BC63869	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23086	WGS84	BC63869	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63869_Mgig_Bot2012_COI	KWIA36	1	BC63869_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63869	Sample	BC63869	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA36	COI	45	BC63869	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA36	TCTTATTCGTTGGAGACTTTATAACCCT	115.75422
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63871_Aamu_Bax2006_COI	KWIA34	1	BC63871_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	GCACAACCGGGATCTTTACTTC	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63871_Pcan_Bot2011_IGS	KWIA34	1	BC63871_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA34	IGS	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	CGTAATCCTCAGTACTGGCTA	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63871_Pper_Dia2013_COI	KWIA34	1	BC63871_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	CTTAGTGGCATTAATTCGDAATCC	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63871_Sspa_Oph2007_COI	KWIA34	1	BC63871_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	GAAGGCCTTCCGATGATC	115.75462
BC63871	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23247	WGS84	BC63871	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63871_Upin_Bot2011_COI	KWIA34	1	BC63871_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63871	Sample	BC63871	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA34	COI	45	BC63871	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA34	CTTTAATTACAGCGTTTTTATTGTTGT	115.75462
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63872_Cjap_Sim2018_COI	KWIA33	1	BC63872_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75344
BC63872	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23229	WGS84	BC63872	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63872_Mgig_Bot2012_COI	KWIA33	1	BC63872_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63872	Sample	BC63872	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA33	COI	45	BC63872	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA33	TCTTATTCGTTGGAGACTTTATAACCCT	115.75344
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63873_Cmae_Bot2011_COI	KWIA32	1	BC63873_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	ATGAACAGTCTATCCTCCTTTAG	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63873_Msal_Bot2012_COI	KWIA32	1	BC63873_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	GYTAGTTCCRATGATGTTAGCTG	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63873_Pvir_Dia2013_COI	KWIA32	1	BC63873_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	CTTAGTGGCATTAATTCGDAATCC	115.75404
BC63873	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23377	WGS84	BC63873	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63873_Rhar_Sim2018_COI	KWIA32	1	BC63873_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63873	Sample	BC63873	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA32	COI	45	BC63873	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA32	CTGCTGCTATTGCTCATG	115.75404
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63875_Aamu_Bax2006_COI	KWIA30	1	BC63875_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	GCACAACCGGGATCTTTACTTC	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63875_Pcan_Bot2011_IGS	KWIA30	1	BC63875_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA30	IGS	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	CGTAATCCTCAGTACTGGCTA	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63875_Pper_Dia2013_COI	KWIA30	1	BC63875_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	CTTAGTGGCATTAATTCGDAATCC	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63875_Sspa_Oph2007_COI	KWIA30	1	BC63875_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	GAAGGCCTTCCGATGATC	115.75568
BC63875	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2362	WGS84	BC63875	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63875_Upin_Bot2011_COI	KWIA30	1	BC63875_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63875	Sample	BC63875	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA30	COI	45	BC63875	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA30	CTTTAATTACAGCGTTTTTATTGTTGT	115.75568
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63876_Cjap_Sim2018_COI	KWIA29	1	BC63876_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75383
BC63876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23582	WGS84	BC63876	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63876_Mgig_Bot2012_COI	KWIA29	1	BC63876_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63876	Sample	BC63876	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA29	COI	45	BC63876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA29	TCTTATTCGTTGGAGACTTTATAACCCT	115.75383
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63877_Cmae_Bot2011_COI	KWIA28	1	BC63877_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	ATGAACAGTCTATCCTCCTTTAG	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63877_Msal_Bot2012_COI	KWIA28	1	BC63877_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	GYTAGTTCCRATGATGTTAGCTG	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63877_Pvir_Dia2013_COI	KWIA28	1	BC63877_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	CTTAGTGGCATTAATTCGDAATCC	115.75828
BC63877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23807	WGS84	BC63877	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63877_Rhar_Sim2018_COI	KWIA28	1	BC63877_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63877	Sample	BC63877	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA28	COI	45	BC63877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA28	CTGCTGCTATTGCTCATG	115.75828
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63879_Aamu_Bax2006_COI	KWIA26	1	BC63879_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	GCACAACCGGGATCTTTACTTC	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63879_Pcan_Bot2011_IGS	KWIA26	1	BC63879_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA26	IGS	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	CGTAATCCTCAGTACTGGCTA	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63879_Pper_Dia2013_COI	KWIA26	1	BC63879_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	CTTAGTGGCATTAATTCGDAATCC	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63879_Sspa_Oph2007_COI	KWIA26	1	BC63879_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	GAAGGCCTTCCGATGATC	115.75454
BC63879	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23742	WGS84	BC63879	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63879_Upin_Bot2011_COI	KWIA26	1	BC63879_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63879	Sample	BC63879	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA26	COI	45	BC63879	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA26	CTTTAATTACAGCGTTTTTATTGTTGT	115.75454
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63880_Cmae_Bot2011_COI	KWIA25	1	BC63880_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	ATGAACAGTCTATCCTCCTTTAG	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63880_Msal_Bot2012_COI	KWIA25	1	BC63880_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	GYTAGTTCCRATGATGTTAGCTG	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63880_Pvir_Dia2013_COI	KWIA25	1	BC63880_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	CTTAGTGGCATTAATTCGDAATCC	115.75253
BC63880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23698	WGS84	BC63880	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63880_Rhar_Sim2018_COI	KWIA25	1	BC63880_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63880	Sample	BC63880	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA25	COI	45	BC63880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA25	CTGCTGCTATTGCTCATG	115.75253
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63882_Aamu_Bax2006_COI	KWIA23	1	BC63882_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	GCACAACCGGGATCTTTACTTC	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63882_Pcan_Bot2011_IGS	KWIA23	1	BC63882_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA23	IGS	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	CGTAATCCTCAGTACTGGCTA	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63882_Pper_Dia2013_COI	KWIA23	1	BC63882_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	CTTAGTGGCATTAATTCGDAATCC	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63882_Sspa_Oph2007_COI	KWIA23	1	BC63882_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	GAAGGCCTTCCGATGATC	115.75331
BC63882	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.23863	WGS84	BC63882	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63882_Upin_Bot2011_COI	KWIA23	1	BC63882_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63882	Sample	BC63882	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA23	COI	45	BC63882	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA23	CTTTAATTACAGCGTTTTTATTGTTGT	115.75331
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63883_Cjap_Sim2018_COI	KWIA22	1	BC63883_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7579
BC63883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24069	WGS84	BC63883	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63883_Mgig_Bot2012_COI	KWIA22	1	BC63883_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63883	Sample	BC63883	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA22	COI	45	BC63883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA22	TCTTATTCGTTGGAGACTTTATAACCCT	115.7579
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63884_Cmae_Bot2011_COI	KWIA21	1	BC63884_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	ATGAACAGTCTATCCTCCTTTAG	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63884_Msal_Bot2012_COI	KWIA21	1	BC63884_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	GYTAGTTCCRATGATGTTAGCTG	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63884_Pvir_Dia2013_COI	KWIA21	1	BC63884_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	CTTAGTGGCATTAATTCGDAATCC	115.75653
BC63884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24053	WGS84	BC63884	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63884_Rhar_Sim2018_COI	KWIA21	1	BC63884_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63884	Sample	BC63884	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA21	COI	45	BC63884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA21	CTGCTGCTATTGCTCATG	115.75653
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63886_Aamu_Bax2006_COI	KWIA19	1	BC63886_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	GCACAACCGGGATCTTTACTTC	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63886_Pcan_Bot2011_IGS	KWIA19	1	BC63886_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA19	IGS	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	CGTAATCCTCAGTACTGGCTA	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63886_Pper_Dia2013_COI	KWIA19	1	BC63886_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	CTTAGTGGCATTAATTCGDAATCC	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63886_Sspa_Oph2007_COI	KWIA19	1	BC63886_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	GAAGGCCTTCCGATGATC	115.75228
BC63886	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24029	WGS84	BC63886	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63886_Upin_Bot2011_COI	KWIA19	1	BC63886_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63886	Sample	BC63886	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA19	COI	45	BC63886	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA19	CTTTAATTACAGCGTTTTTATTGTTGT	115.75228
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63887_Cjap_Sim2018_COI	KWIA18	1	BC63887_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.757
BC63887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24247	WGS84	BC63887	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63887_Mgig_Bot2012_COI	KWIA18	1	BC63887_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63887	Sample	BC63887	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA18	COI	45	BC63887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA18	TCTTATTCGTTGGAGACTTTATAACCCT	115.757
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63888_Cmae_Bot2011_COI	KWIA17	1	BC63888_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	ATGAACAGTCTATCCTCCTTTAG	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63888_Msal_Bot2012_COI	KWIA17	1	BC63888_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	GYTAGTTCCRATGATGTTAGCTG	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63888_Pvir_Dia2013_COI	KWIA17	1	BC63888_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	CTTAGTGGCATTAATTCGDAATCC	115.75289
BC63888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24173	WGS84	BC63888	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63888_Rhar_Sim2018_COI	KWIA17	1	BC63888_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63888	Sample	BC63888	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA17	COI	45	BC63888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA17	CTGCTGCTATTGCTCATG	115.75289
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63890_Cjap_Sim2018_COI	KWIA15	1	BC63890_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75557
BC63890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24367	WGS84	BC63890	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63890_Mgig_Bot2012_COI	KWIA15	1	BC63890_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63890	Sample	BC63890	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA15	COI	45	BC63890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA15	TCTTATTCGTTGGAGACTTTATAACCCT	115.75557
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63891_Cmae_Bot2011_COI	KWIA14	1	BC63891_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	ATGAACAGTCTATCCTCCTTTAG	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63891_Msal_Bot2012_COI	KWIA14	1	BC63891_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	GYTAGTTCCRATGATGTTAGCTG	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63891_Pvir_Dia2013_COI	KWIA14	1	BC63891_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	CTTAGTGGCATTAATTCGDAATCC	115.75365
BC63891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24337	WGS84	BC63891	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63891_Rhar_Sim2018_COI	KWIA14	1	BC63891_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63891	Sample	BC63891	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA14	COI	45	BC63891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA14	CTGCTGCTATTGCTCATG	115.75365
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63893_Aamu_Bax2006_COI	KWIA12	1	BC63893_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	GCACAACCGGGATCTTTACTTC	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63893_Pcan_Bot2011_IGS	KWIA12	1	BC63893_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA12	IGS	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	CGTAATCCTCAGTACTGGCTA	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63893_Pper_Dia2013_COI	KWIA12	1	BC63893_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	CTTAGTGGCATTAATTCGDAATCC	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63893_Sspa_Oph2007_COI	KWIA12	1	BC63893_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	GAAGGCCTTCCGATGATC	115.75246
BC63893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.24459	WGS84	BC63893	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63893_Upin_Bot2011_COI	KWIA12	1	BC63893_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63893	Sample	BC63893	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA12	COI	45	BC63893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA12	CTTTAATTACAGCGTTTTTATTGTTGT	115.75246
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63894_Cjap_Sim2018_COI	KWIA11	1	BC63894_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74442
BC63894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.2527	WGS84	BC63894	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63894_Mgig_Bot2012_COI	KWIA11	1	BC63894_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63894	Sample	BC63894	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA11	COI	45	BC63894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA11	TCTTATTCGTTGGAGACTTTATAACCCT	115.74442
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63895_Cmae_Bot2011_COI	KWIA10	1	BC63895_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	ATGAACAGTCTATCCTCCTTTAG	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63895_Msal_Bot2012_COI	KWIA10	1	BC63895_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	GYTAGTTCCRATGATGTTAGCTG	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63895_Pvir_Dia2013_COI	KWIA10	1	BC63895_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	CTTAGTGGCATTAATTCGDAATCC	115.74309
BC63895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25262	WGS84	BC63895	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63895_Rhar_Sim2018_COI	KWIA10	1	BC63895_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63895	Sample	BC63895	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA10	COI	45	BC63895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA10	CTGCTGCTATTGCTCATG	115.74309
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63897_Aamu_Bax2006_COI	KWIA8	1	BC63897_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	GCACAACCGGGATCTTTACTTC	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63897_Pcan_Bot2011_IGS	KWIA8	1	BC63897_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA8	IGS	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	CGTAATCCTCAGTACTGGCTA	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63897_Pper_Dia2013_COI	KWIA8	1	BC63897_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	CTTAGTGGCATTAATTCGDAATCC	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63897_Sspa_Oph2007_COI	KWIA8	1	BC63897_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	GAAGGCCTTCCGATGATC	115.74373
BC63897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25329	WGS84	BC63897	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63897_Upin_Bot2011_COI	KWIA8	1	BC63897_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63897	Sample	BC63897	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA8	COI	45	BC63897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA8	CTTTAATTACAGCGTTTTTATTGTTGT	115.74373
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63898_Cjap_Sim2018_COI	KWIA7	1	BC63898_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74208
BC63898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25375	WGS84	BC63898	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63898_Mgig_Bot2012_COI	KWIA7	1	BC63898_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63898	Sample	BC63898	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA7	COI	45	BC63898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA7	TCTTATTCGTTGGAGACTTTATAACCCT	115.74208
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63899_Cmae_Bot2011_COI	KWIA6	1	BC63899_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	ATGAACAGTCTATCCTCCTTTAG	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63899_Msal_Bot2012_COI	KWIA6	1	BC63899_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	GYTAGTTCCRATGATGTTAGCTG	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63899_Pvir_Dia2013_COI	KWIA6	1	BC63899_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	CTTAGTGGCATTAATTCGDAATCC	115.74095
BC63899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25386	WGS84	BC63899	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63899_Rhar_Sim2018_COI	KWIA6	1	BC63899_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63899	Sample	BC63899	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA6	COI	45	BC63899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA6	CTGCTGCTATTGCTCATG	115.74095
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63901_Aamu_Bax2006_COI	KWIA4	1	BC63901_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Aamu_Bax2006_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	GCACAACCGGGATCTTTACTTC	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63901_Pcan_Bot2011_IGS	KWIA4	1	BC63901_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Pcan_Bot2011_IGS	KWIA4	IGS	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	CGTAATCCTCAGTACTGGCTA	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63901_Pper_Dia2013_COI	KWIA4	1	BC63901_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Pper_Dia2013_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	CTTAGTGGCATTAATTCGDAATCC	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63901_Sspa_Oph2007_COI	KWIA4	1	BC63901_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Sspa_Oph2007_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	GAAGGCCTTCCGATGATC	115.74282
BC63901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25511	WGS84	BC63901	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63901_Upin_Bot2011_COI	KWIA4	1	BC63901_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63901	Sample	BC63901	targeted	Absent	Sample	Upin_Bot2011_COI	KWIA4	COI	45	BC63901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA4	CTTTAATTACAGCGTTTTTATTGTTGT	115.74282
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63902_Cjap_Sim2018_COI	KWIA3	1	BC63902_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Cjap_Sim2018_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74126
BC63902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25493	WGS84	BC63902	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63902_Mgig_Bot2012_COI	KWIA3	1	BC63902_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63902	Sample	BC63902	targeted	Absent	Sample	Mgig_Bot2012_COI	KWIA3	COI	45	BC63902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA3	TCTTATTCGTTGGAGACTTTATAACCCT	115.74126
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63903_Cmae_Bot2011_COI	KWIA2	1	BC63903_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Cmae_Bot2011_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	ATGAACAGTCTATCCTCCTTTAG	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63903_Msal_Bot2012_COI	KWIA2	1	BC63903_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Msal_Bot2012_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	GYTAGTTCCRATGATGTTAGCTG	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63903_Pvir_Dia2013_COI	KWIA2	1	BC63903_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Pvir_Dia2013_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	CTTAGTGGCATTAATTCGDAATCC	115.74164
BC63903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-32.25595	WGS84	BC63903	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Kwinana_Summer	19/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63903_Rhar_Sim2018_COI	KWIA2	1	BC63903_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63903	Sample	BC63903	targeted	Absent	Sample	Rhar_Sim2018_COI	KWIA2	COI	45	BC63903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KWIA2	CTGCTGCTATTGCTCATG	115.74164
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63905_Aamu_Bax2006_COI	FREA32	1	BC63905_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	GCACAACCGGGATCTTTACTTC	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63905_Pcan_Bot2011_IGS	FREA32	1	BC63905_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA32	IGS	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	CGTAATCCTCAGTACTGGCTA	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63905_Pper_Dia2013_COI	FREA32	1	BC63905_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Pper_Dia2013_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	CTTAGTGGCATTAATTCGDAATCC	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63905_Sspa_Oph2007_COI	FREA32	1	BC63905_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	GAAGGCCTTCCGATGATC	115.75126
BC63905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04051	WGS84	BC63905	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63905_Upin_Bot2011_COI	FREA32	1	BC63905_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63905	Sample	BC63905	targeted	Absent	Sample	Upin_Bot2011_COI	FREA32	COI	45	BC63905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA32	CTTTAATTACAGCGTTTTTATTGTTGT	115.75126
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63906_Cjap_Sim2018_COI	FREA33	1	BC63906_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.75228
BC63906	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04116	WGS84	BC63906	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63906_Mgig_Bot2012_COI	FREA33	1	BC63906_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63906	Sample	BC63906	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA33	COI	45	BC63906	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA33	TCTTATTCGTTGGAGACTTTATAACCCT	115.75228
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63907_Cmae_Bot2011_COI	FREA5	1	BC63907_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	ATGAACAGTCTATCCTCCTTTAG	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63907_Hsan_Wil2021_COI	FREA5	1	BC63907_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	CCTGGGCCGGTATAGTAGGT	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63907_Mros_Gib2022_COI	FREA5	1	BC63907_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Mros_Gib2022_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63907_Msal_Bot2012_COI	FREA5	1	BC63907_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Msal_Bot2012_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	GYTAGTTCCRATGATGTTAGCTG	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63907_Pvir_Dia2013_COI	FREA5	1	BC63907_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	CTTAGTGGCATTAATTCGDAATCC	115.74059
BC63907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05092	WGS84	BC63907	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63907_Rhar_Sim2018_COI	FREA5	1	BC63907_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63907	Sample	BC63907	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA5	COI	45	BC63907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA5	CTGCTGCTATTGCTCATG	115.74059
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63909_Aamu_Bax2006_COI	FREA15	1	BC63909_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	GCACAACCGGGATCTTTACTTC	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63909_Pcan_Bot2011_IGS	FREA15	1	BC63909_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA15	IGS	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	CGTAATCCTCAGTACTGGCTA	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63909_Pper_Dia2013_COI	FREA15	1	BC63909_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Pper_Dia2013_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	CTTAGTGGCATTAATTCGDAATCC	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63909_Sspa_Oph2007_COI	FREA15	1	BC63909_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	GAAGGCCTTCCGATGATC	115.74348
BC63909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04636	WGS84	BC63909	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63909_Upin_Bot2011_COI	FREA15	1	BC63909_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63909	Sample	BC63909	targeted	Absent	Sample	Upin_Bot2011_COI	FREA15	COI	45	BC63909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA15	CTTTAATTACAGCGTTTTTATTGTTGT	115.74348
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63910_Cmae_Bot2011_COI	FREA20	1	BC63910_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	ATGAACAGTCTATCCTCCTTTAG	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63910_Hsan_Wil2021_COI	FREA20	1	BC63910_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	CCTGGGCCGGTATAGTAGGT	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63910_Mros_Gib2022_COI	FREA20	1	BC63910_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Mros_Gib2022_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63910_Msal_Bot2012_COI	FREA20	1	BC63910_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Msal_Bot2012_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	GYTAGTTCCRATGATGTTAGCTG	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63910_Pvir_Dia2013_COI	FREA20	1	BC63910_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	CTTAGTGGCATTAATTCGDAATCC	115.74761
BC63910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04563	WGS84	BC63910	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63910_Rhar_Sim2018_COI	FREA20	1	BC63910_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63910	Sample	BC63910	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA20	COI	45	BC63910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA20	CTGCTGCTATTGCTCATG	115.74761
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63912_Aamu_Bax2006_COI	FREA30	1	BC63912_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	GCACAACCGGGATCTTTACTTC	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63912_Pcan_Bot2011_IGS	FREA30	1	BC63912_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA30	IGS	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	CGTAATCCTCAGTACTGGCTA	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63912_Pper_Dia2013_COI	FREA30	1	BC63912_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Pper_Dia2013_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	CTTAGTGGCATTAATTCGDAATCC	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63912_Sspa_Oph2007_COI	FREA30	1	BC63912_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	GAAGGCCTTCCGATGATC	115.75018
BC63912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04129	WGS84	BC63912	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63912_Upin_Bot2011_COI	FREA30	1	BC63912_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63912	Sample	BC63912	targeted	Absent	Sample	Upin_Bot2011_COI	FREA30	COI	45	BC63912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA30	CTTTAATTACAGCGTTTTTATTGTTGT	115.75018
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63913_Cjap_Sim2018_COI	FREA1	1	BC63913_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63913_Esin_And2018_Cyb	FREA1	1	BC63913_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Esin_And2018_Cyb	FREA1	Cytb	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	ACCCCTCCTCATATCCAACCA	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63913_Mgig_Bot2012_COI	FREA1	1	BC63913_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	TCTTATTCGTTGGAGACTTTATAACCCT	115.73752
BC63913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05449	WGS84	BC63913	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63913_Mjap_Gib2022_COI	FREA1	1	BC63913_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63913	Sample	BC63913	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA1	COI	45	BC63913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA1	AATTTAATTGTGACTAGGCATGGTCTG	115.73752
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63914_Cmae_Bot2011_COI	FREA2	1	BC63914_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	ATGAACAGTCTATCCTCCTTTAG	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63914_Hsan_Wil2021_COI	FREA2	1	BC63914_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	CCTGGGCCGGTATAGTAGGT	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63914_Mros_Gib2022_COI	FREA2	1	BC63914_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Mros_Gib2022_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63914_Msal_Bot2012_COI	FREA2	1	BC63914_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Msal_Bot2012_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	GYTAGTTCCRATGATGTTAGCTG	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63914_Pvir_Dia2013_COI	FREA2	1	BC63914_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	CTTAGTGGCATTAATTCGDAATCC	115.73877
BC63914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05334	WGS84	BC63914	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63914_Rhar_Sim2018_COI	FREA2	1	BC63914_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63914	Sample	BC63914	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA2	COI	45	BC63914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA2	CTGCTGCTATTGCTCATG	115.73877
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63916_Aamu_Bax2006_COI	FREA4	1	BC63916_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	GCACAACCGGGATCTTTACTTC	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63916_Pcan_Bot2011_IGS	FREA4	1	BC63916_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA4	IGS	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	CGTAATCCTCAGTACTGGCTA	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63916_Pper_Dia2013_COI	FREA4	1	BC63916_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Pper_Dia2013_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	CTTAGTGGCATTAATTCGDAATCC	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63916_Sspa_Oph2007_COI	FREA4	1	BC63916_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	GAAGGCCTTCCGATGATC	115.74132
BC63916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05239	WGS84	BC63916	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63916_Upin_Bot2011_COI	FREA4	1	BC63916_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63916	Sample	BC63916	targeted	Absent	Sample	Upin_Bot2011_COI	FREA4	COI	45	BC63916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA4	CTTTAATTACAGCGTTTTTATTGTTGT	115.74132
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63917_Cjap_Sim2018_COI	FREA6	1	BC63917_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63917_Esin_And2018_Cyb	FREA6	1	BC63917_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Esin_And2018_Cyb	FREA6	Cytb	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	ACCCCTCCTCATATCCAACCA	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63917_Mgig_Bot2012_COI	FREA6	1	BC63917_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	TCTTATTCGTTGGAGACTTTATAACCCT	115.74243
BC63917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.05116	WGS84	BC63917	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63917_Mjap_Gib2022_COI	FREA6	1	BC63917_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63917	Sample	BC63917	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA6	COI	45	BC63917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA6	AATTTAATTGTGACTAGGCATGGTCTG	115.74243
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63918_Cmae_Bot2011_COI	FREA7	1	BC63918_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	ATGAACAGTCTATCCTCCTTTAG	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63918_Hsan_Wil2021_COI	FREA7	1	BC63918_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	CCTGGGCCGGTATAGTAGGT	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63918_Mros_Gib2022_COI	FREA7	1	BC63918_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Mros_Gib2022_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63918_Msal_Bot2012_COI	FREA7	1	BC63918_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Msal_Bot2012_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	GYTAGTTCCRATGATGTTAGCTG	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63918_Pvir_Dia2013_COI	FREA7	1	BC63918_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	CTTAGTGGCATTAATTCGDAATCC	115.74189
BC63918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04972	WGS84	BC63918	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63918_Rhar_Sim2018_COI	FREA7	1	BC63918_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63918	Sample	BC63918	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA7	COI	45	BC63918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA7	CTGCTGCTATTGCTCATG	115.74189
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63920_Cjap_Sim2018_COI	FREA9	1	BC63920_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63920_Esin_And2018_Cyb	FREA9	1	BC63920_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Esin_And2018_Cyb	FREA9	Cytb	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	ACCCCTCCTCATATCCAACCA	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63920_Mgig_Bot2012_COI	FREA9	1	BC63920_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	TCTTATTCGTTGGAGACTTTATAACCCT	115.74142
BC63920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04868	WGS84	BC63920	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63920_Mjap_Gib2022_COI	FREA9	1	BC63920_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63920	Sample	BC63920	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA9	COI	45	BC63920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA9	AATTTAATTGTGACTAGGCATGGTCTG	115.74142
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63921_Cmae_Bot2011_COI	FREA11	1	BC63921_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	ATGAACAGTCTATCCTCCTTTAG	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63921_Hsan_Wil2021_COI	FREA11	1	BC63921_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	CCTGGGCCGGTATAGTAGGT	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63921_Mros_Gib2022_COI	FREA11	1	BC63921_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Mros_Gib2022_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63921_Msal_Bot2012_COI	FREA11	1	BC63921_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Msal_Bot2012_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	GYTAGTTCCRATGATGTTAGCTG	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63921_Pvir_Dia2013_COI	FREA11	1	BC63921_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	CTTAGTGGCATTAATTCGDAATCC	115.74474
BC63921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0488	WGS84	BC63921	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63921_Rhar_Sim2018_COI	FREA11	1	BC63921_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63921	Sample	BC63921	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA11	COI	45	BC63921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA11	CTGCTGCTATTGCTCATG	115.74474
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63923_Aamu_Bax2006_COI	FREA13	1	BC63923_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	GCACAACCGGGATCTTTACTTC	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63923_Pcan_Bot2011_IGS	FREA13	1	BC63923_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA13	IGS	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	CGTAATCCTCAGTACTGGCTA	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63923_Pper_Dia2013_COI	FREA13	1	BC63923_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Pper_Dia2013_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	CTTAGTGGCATTAATTCGDAATCC	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63923_Sspa_Oph2007_COI	FREA13	1	BC63923_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	GAAGGCCTTCCGATGATC	115.7438
BC63923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0476	WGS84	BC63923	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63923_Upin_Bot2011_COI	FREA13	1	BC63923_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63923	Sample	BC63923	targeted	Absent	Sample	Upin_Bot2011_COI	FREA13	COI	45	BC63923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA13	CTTTAATTACAGCGTTTTTATTGTTGT	115.7438
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63924_Cjap_Sim2018_COI	FREA14	1	BC63924_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63924_Esin_And2018_Cyb	FREA14	1	BC63924_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Esin_And2018_Cyb	FREA14	Cytb	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	ACCCCTCCTCATATCCAACCA	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63924_Mgig_Bot2012_COI	FREA14	1	BC63924_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	TCTTATTCGTTGGAGACTTTATAACCCT	115.74543
BC63924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04771	WGS84	BC63924	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63924_Mjap_Gib2022_COI	FREA14	1	BC63924_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63924	Sample	BC63924	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA14	COI	45	BC63924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA14	AATTTAATTGTGACTAGGCATGGTCTG	115.74543
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63925_Cmae_Bot2011_COI	FREA16	1	BC63925_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	ATGAACAGTCTATCCTCCTTTAG	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63925_Hsan_Wil2021_COI	FREA16	1	BC63925_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	CCTGGGCCGGTATAGTAGGT	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63925_Mros_Gib2022_COI	FREA16	1	BC63925_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Mros_Gib2022_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63925_Msal_Bot2012_COI	FREA16	1	BC63925_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Msal_Bot2012_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	GYTAGTTCCRATGATGTTAGCTG	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63925_Pvir_Dia2013_COI	FREA16	1	BC63925_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	CTTAGTGGCATTAATTCGDAATCC	115.74466
BC63925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04633	WGS84	BC63925	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63925_Rhar_Sim2018_COI	FREA16	1	BC63925_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63925	Sample	BC63925	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA16	COI	45	BC63925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA16	CTGCTGCTATTGCTCATG	115.74466
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63927_Aamu_Bax2006_COI	FREA18	1	BC63927_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	GCACAACCGGGATCTTTACTTC	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63927_Pcan_Bot2011_IGS	FREA18	1	BC63927_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA18	IGS	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	CGTAATCCTCAGTACTGGCTA	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63927_Pper_Dia2013_COI	FREA18	1	BC63927_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Pper_Dia2013_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	CTTAGTGGCATTAATTCGDAATCC	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63927_Sspa_Oph2007_COI	FREA18	1	BC63927_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	GAAGGCCTTCCGATGATC	115.74433
BC63927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04525	WGS84	BC63927	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63927_Upin_Bot2011_COI	FREA18	1	BC63927_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63927	Sample	BC63927	targeted	Absent	Sample	Upin_Bot2011_COI	FREA18	COI	45	BC63927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA18	CTTTAATTACAGCGTTTTTATTGTTGT	115.74433
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63928_Cjap_Sim2018_COI	FREA19	1	BC63928_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63928_Esin_And2018_Cyb	FREA19	1	BC63928_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Esin_And2018_Cyb	FREA19	Cytb	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	ACCCCTCCTCATATCCAACCA	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63928_Mgig_Bot2012_COI	FREA19	1	BC63928_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	TCTTATTCGTTGGAGACTTTATAACCCT	115.74597
BC63928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04527	WGS84	BC63928	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63928_Mjap_Gib2022_COI	FREA19	1	BC63928_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63928	Sample	BC63928	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA19	COI	45	BC63928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA19	AATTTAATTGTGACTAGGCATGGTCTG	115.74597
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63929_Cmae_Bot2011_COI	FREA17	1	BC63929_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	ATGAACAGTCTATCCTCCTTTAG	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63929_Hsan_Wil2021_COI	FREA17	1	BC63929_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Hsan_Wil2021_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	CCTGGGCCGGTATAGTAGGT	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63929_Mros_Gib2022_COI	FREA17	1	BC63929_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Mros_Gib2022_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63929_Msal_Bot2012_COI	FREA17	1	BC63929_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Msal_Bot2012_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	GYTAGTTCCRATGATGTTAGCTG	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63929_Pvir_Dia2013_COI	FREA17	1	BC63929_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	CTTAGTGGCATTAATTCGDAATCC	115.74646
BC63929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0467	WGS84	BC63929	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63929_Rhar_Sim2018_COI	FREA17	1	BC63929_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63929	Sample	BC63929	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA17	COI	45	BC63929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA17	CTGCTGCTATTGCTCATG	115.74646
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63930_Aamu_Bax2006_COI	FREA22	1	BC63930_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	GCACAACCGGGATCTTTACTTC	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63930_Pcan_Bot2011_IGS	FREA22	1	BC63930_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA22	IGS	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	CGTAATCCTCAGTACTGGCTA	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63930_Pper_Dia2013_COI	FREA22	1	BC63930_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Pper_Dia2013_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	CTTAGTGGCATTAATTCGDAATCC	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63930_Sspa_Oph2007_COI	FREA22	1	BC63930_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	GAAGGCCTTCCGATGATC	115.74689
BC63930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04452	WGS84	BC63930	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63930_Upin_Bot2011_COI	FREA22	1	BC63930_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63930	Sample	BC63930	targeted	Absent	Sample	Upin_Bot2011_COI	FREA22	COI	45	BC63930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA22	CTTTAATTACAGCGTTTTTATTGTTGT	115.74689
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63931_Cjap_Sim2018_COI	FREA23	1	BC63931_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63931_Esin_And2018_Cyb	FREA23	1	BC63931_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Esin_And2018_Cyb	FREA23	Cytb	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	ACCCCTCCTCATATCCAACCA	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63931_Mgig_Bot2012_COI	FREA23	1	BC63931_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	TCTTATTCGTTGGAGACTTTATAACCCT	115.7487
BC63931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04468	WGS84	BC63931	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63931_Mjap_Gib2022_COI	FREA23	1	BC63931_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63931	Sample	BC63931	targeted	Absent	Sample	Mjap_Gib2022_COI	FREA23	COI	45	BC63931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA23	AATTTAATTGTGACTAGGCATGGTCTG	115.7487
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63932_Cmae_Bot2011_COI	FREA24	1	BC63932_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	ATGAACAGTCTATCCTCCTTTAG	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63932_Msal_Bot2012_COI	FREA24	1	BC63932_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Msal_Bot2012_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	GYTAGTTCCRATGATGTTAGCTG	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63932_Pvir_Dia2013_COI	FREA24	1	BC63932_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	CTTAGTGGCATTAATTCGDAATCC	115.74651
BC63932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04333	WGS84	BC63932	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63932_Rhar_Sim2018_COI	FREA24	1	BC63932_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63932	Sample	BC63932	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA24	COI	45	BC63932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA24	CTGCTGCTATTGCTCATG	115.74651
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63934_Aamu_Bax2006_COI	FREA27	1	BC63934_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Aamu_Bax2006_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	GCACAACCGGGATCTTTACTTC	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63934_Pcan_Bot2011_IGS	FREA27	1	BC63934_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Pcan_Bot2011_IGS	FREA27	IGS	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	CGTAATCCTCAGTACTGGCTA	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63934_Pper_Dia2013_COI	FREA27	1	BC63934_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Pper_Dia2013_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	CTTAGTGGCATTAATTCGDAATCC	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63934_Sspa_Oph2007_COI	FREA27	1	BC63934_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Sspa_Oph2007_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	GAAGGCCTTCCGATGATC	115.74776
BC63934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04256	WGS84	BC63934	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63934_Upin_Bot2011_COI	FREA27	1	BC63934_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63934	Sample	BC63934	targeted	Absent	Sample	Upin_Bot2011_COI	FREA27	COI	45	BC63934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA27	CTTTAATTACAGCGTTTTTATTGTTGT	115.74776
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63935_Cjap_Sim2018_COI	FREA28	1	BC63935_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Cjap_Sim2018_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.7493
BC63935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.04257	WGS84	BC63935	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63935_Mgig_Bot2012_COI	FREA28	1	BC63935_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63935	Sample	BC63935	targeted	Absent	Sample	Mgig_Bot2012_COI	FREA28	COI	45	BC63935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA28	TCTTATTCGTTGGAGACTTTATAACCCT	115.7493
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63936_Cmae_Bot2011_COI	FREA29	1	BC63936_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Cmae_Bot2011_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	ATGAACAGTCTATCCTCCTTTAG	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63936_Msal_Bot2012_COI	FREA29	1	BC63936_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Msal_Bot2012_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	GYTAGTTCCRATGATGTTAGCTG	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63936_Pvir_Dia2013_COI	FREA29	1	BC63936_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Pvir_Dia2013_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	CTTAGTGGCATTAATTCGDAATCC	115.75084
BC63936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-32.0426	WGS84	BC63936	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Fremantle_Summer	17/03/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63936_Rhar_Sim2018_COI	FREA29	1	BC63936_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63936	Sample	BC63936	targeted	Absent	Sample	Rhar_Sim2018_COI	FREA29	COI	45	BC63936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	FREA29	CTGCTGCTATTGCTCATG	115.75084
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63938_Aamu_Bax2006_COI	BUNA23	1	BC63938_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	GCACAACCGGGATCTTTACTTC	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63938_Pcan_Bot2011_IGS	BUNA23	1	BC63938_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA23	IGS	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	CGTAATCCTCAGTACTGGCTA	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63938_Pper_Dia2013_COI	BUNA23	1	BC63938_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	CTTAGTGGCATTAATTCGDAATCC	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63938_Sspa_Oph2007_COI	BUNA23	1	BC63938_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	GAAGGCCTTCCGATGATC	115.64785
BC63938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30573	WGS84	BC63938	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63938_Upin_Bot2011_COI	BUNA23	1	BC63938_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63938	Sample	BC63938	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA23	COI	45	BC63938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA23	CTTTAATTACAGCGTTTTTATTGTTGT	115.64785
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63939_Asen_Wil2025_COI	BUNA22	1	BC63939_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	GTTGTTGTTACAACACATGCTCTTTTG	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63939_Cjap_Sim2018_COI	BUNA22	1	BC63939_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63939_Esin_And2018_Cyb	BUNA22	1	BC63939_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA22	Cytb	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	ACCCCTCCTCATATCCAACCA	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63939_Mgig_Bot2012_COI	BUNA22	1	BC63939_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	TCTTATTCGTTGGAGACTTTATAACCCT	115.64643
BC63939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30589	WGS84	BC63939	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63939_Mjap_Gib2022_COI	BUNA22	1	BC63939_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63939	Sample	BC63939	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA22	COI	45	BC63939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA22	AATTTAATTGTGACTAGGCATGGTCTG	115.64643
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63941_Aamu_Bax2006_COI	BUNA20	1	BC63941_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	GCACAACCGGGATCTTTACTTC	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63941_Pcan_Bot2011_IGS	BUNA20	1	BC63941_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA20	IGS	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	CGTAATCCTCAGTACTGGCTA	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63941_Pper_Dia2013_COI	BUNA20	1	BC63941_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	CTTAGTGGCATTAATTCGDAATCC	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63941_Sspa_Oph2007_COI	BUNA20	1	BC63941_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	GAAGGCCTTCCGATGATC	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63941_Upin_Bot2011_COI	BUNA20	1	BC63941_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	CTTTAATTACAGCGTTTTTATTGTTGT	115.64524
BC63941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30703	WGS84	BC63941	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63941_Vgib_Wil2025_COI	BUNA20	1	BC63941_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63941	Sample	BC63941	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA20	COI	45	BC63941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA20	CCCCTCTTTACAAGTTCTTTAGGGT	115.64524
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63942_Asen_Wil2025_COI	BUNA19	1	BC63942_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	GTTGTTGTTACAACACATGCTCTTTTG	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63942_Cjap_Sim2018_COI	BUNA19	1	BC63942_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63942_Esin_And2018_Cyb	BUNA19	1	BC63942_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA19	Cytb	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	ACCCCTCCTCATATCCAACCA	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63942_Mgig_Bot2012_COI	BUNA19	1	BC63942_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	TCTTATTCGTTGGAGACTTTATAACCCT	115.64384
BC63942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30814	WGS84	BC63942	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63942_Mjap_Gib2022_COI	BUNA19	1	BC63942_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63942	Sample	BC63942	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA19	COI	45	BC63942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA19	AATTTAATTGTGACTAGGCATGGTCTG	115.64384
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63943_Cmae_Bot2011_COI	BUNA18	1	BC63943_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	ATGAACAGTCTATCCTCCTTTAG	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63943_Hsan_Wil2021_COI	BUNA18	1	BC63943_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	CCTGGGCCGGTATAGTAGGT	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63943_Mros_Gib2022_COI	BUNA18	1	BC63943_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63943_Msal_Bot2012_COI	BUNA18	1	BC63943_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	GYTAGTTCCRATGATGTTAGCTG	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63943_Pvir_Dia2013_COI	BUNA18	1	BC63943_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	CTTAGTGGCATTAATTCGDAATCC	115.64241
BC63943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.30924	WGS84	BC63943	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63943_Rhar_Sim2018_COI	BUNA18	1	BC63943_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63943	Sample	BC63943	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA18	COI	45	BC63943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA18	CTGCTGCTATTGCTCATG	115.64241
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63945_Aamu_Bax2006_COI	BUNA16	1	BC63945_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	GCACAACCGGGATCTTTACTTC	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63945_Pcan_Bot2011_IGS	BUNA16	1	BC63945_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA16	IGS	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	CGTAATCCTCAGTACTGGCTA	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63945_Pper_Dia2013_COI	BUNA16	1	BC63945_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	CTTAGTGGCATTAATTCGDAATCC	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63945_Sspa_Oph2007_COI	BUNA16	1	BC63945_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	GAAGGCCTTCCGATGATC	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63945_Upin_Bot2011_COI	BUNA16	1	BC63945_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	CTTTAATTACAGCGTTTTTATTGTTGT	115.66118
BC63945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32049	WGS84	BC63945	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63945_Vgib_Wil2025_COI	BUNA16	1	BC63945_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63945	Sample	BC63945	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA16	COI	45	BC63945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA16	CCCCTCTTTACAAGTTCTTTAGGGT	115.66118
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63946_Asen_Wil2025_COI	BUNA15	1	BC63946_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	GTTGTTGTTACAACACATGCTCTTTTG	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63946_Cjap_Sim2018_COI	BUNA15	1	BC63946_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63946_Esin_And2018_Cyb	BUNA15	1	BC63946_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA15	Cytb	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	ACCCCTCCTCATATCCAACCA	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63946_Mgig_Bot2012_COI	BUNA15	1	BC63946_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	TCTTATTCGTTGGAGACTTTATAACCCT	115.66542
BC63946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32276	WGS84	BC63946	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63946_Mjap_Gib2022_COI	BUNA15	1	BC63946_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63946	Sample	BC63946	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA15	COI	45	BC63946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA15	AATTTAATTGTGACTAGGCATGGTCTG	115.66542
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63947_Cmae_Bot2011_COI	BUNA14	1	BC63947_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	ATGAACAGTCTATCCTCCTTTAG	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63947_Hsan_Wil2021_COI	BUNA14	1	BC63947_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	CCTGGGCCGGTATAGTAGGT	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63947_Mros_Gib2022_COI	BUNA14	1	BC63947_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63947_Msal_Bot2012_COI	BUNA14	1	BC63947_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	GYTAGTTCCRATGATGTTAGCTG	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63947_Pvir_Dia2013_COI	BUNA14	1	BC63947_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	CTTAGTGGCATTAATTCGDAATCC	115.66383
BC63947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32204	WGS84	BC63947	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63947_Rhar_Sim2018_COI	BUNA14	1	BC63947_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63947	Sample	BC63947	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA14	COI	45	BC63947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA14	CTGCTGCTATTGCTCATG	115.66383
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63949_Aamu_Bax2006_COI	BUNA12	1	BC63949_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	GCACAACCGGGATCTTTACTTC	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63949_Pcan_Bot2011_IGS	BUNA12	1	BC63949_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA12	IGS	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	CGTAATCCTCAGTACTGGCTA	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63949_Pper_Dia2013_COI	BUNA12	1	BC63949_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	CTTAGTGGCATTAATTCGDAATCC	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63949_Sspa_Oph2007_COI	BUNA12	1	BC63949_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	GAAGGCCTTCCGATGATC	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63949_Upin_Bot2011_COI	BUNA12	1	BC63949_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	CTTTAATTACAGCGTTTTTATTGTTGT	115.66024
BC63949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32164	WGS84	BC63949	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63949_Vgib_Wil2025_COI	BUNA12	1	BC63949_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63949	Sample	BC63949	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA12	COI	45	BC63949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA12	CCCCTCTTTACAAGTTCTTTAGGGT	115.66024
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63950_Cmae_Bot2011_COI	BUNA11	1	BC63950_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	ATGAACAGTCTATCCTCCTTTAG	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63950_Hsan_Wil2021_COI	BUNA11	1	BC63950_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	CCTGGGCCGGTATAGTAGGT	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63950_Mros_Gib2022_COI	BUNA11	1	BC63950_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63950_Msal_Bot2012_COI	BUNA11	1	BC63950_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	GYTAGTTCCRATGATGTTAGCTG	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63950_Pvir_Dia2013_COI	BUNA11	1	BC63950_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	CTTAGTGGCATTAATTCGDAATCC	115.65884
BC63950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32104	WGS84	BC63950	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63950_Rhar_Sim2018_COI	BUNA11	1	BC63950_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63950	Sample	BC63950	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA11	COI	45	BC63950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA11	CTGCTGCTATTGCTCATG	115.65884
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63952_Aamu_Bax2006_COI	BUNA9	1	BC63952_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	GCACAACCGGGATCTTTACTTC	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63952_Pcan_Bot2011_IGS	BUNA9	1	BC63952_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA9	IGS	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	CGTAATCCTCAGTACTGGCTA	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63952_Pper_Dia2013_COI	BUNA9	1	BC63952_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	CTTAGTGGCATTAATTCGDAATCC	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63952_Sspa_Oph2007_COI	BUNA9	1	BC63952_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	GAAGGCCTTCCGATGATC	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63952_Upin_Bot2011_COI	BUNA9	1	BC63952_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	CTTTAATTACAGCGTTTTTATTGTTGT	115.66476
BC63952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32321	WGS84	BC63952	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63952_Vgib_Wil2025_COI	BUNA9	1	BC63952_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63952	Sample	BC63952	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA9	COI	45	BC63952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA9	CCCCTCTTTACAAGTTCTTTAGGGT	115.66476
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63953_Asen_Wil2025_COI	BUNA8	1	BC63953_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	GTTGTTGTTACAACACATGCTCTTTTG	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63953_Cjap_Sim2018_COI	BUNA8	1	BC63953_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63953_Esin_And2018_Cyb	BUNA8	1	BC63953_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA8	Cytb	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	ACCCCTCCTCATATCCAACCA	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63953_Mgig_Bot2012_COI	BUNA8	1	BC63953_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	TCTTATTCGTTGGAGACTTTATAACCCT	115.66291
BC63953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.323	WGS84	BC63953	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63953_Mjap_Gib2022_COI	BUNA8	1	BC63953_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63953	Sample	BC63953	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA8	COI	45	BC63953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA8	AATTTAATTGTGACTAGGCATGGTCTG	115.66291
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63954_Cmae_Bot2011_COI	BUNA7	1	BC63954_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	ATGAACAGTCTATCCTCCTTTAG	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63954_Hsan_Wil2021_COI	BUNA7	1	BC63954_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	CCTGGGCCGGTATAGTAGGT	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63954_Mros_Gib2022_COI	BUNA7	1	BC63954_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63954_Msal_Bot2012_COI	BUNA7	1	BC63954_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	GYTAGTTCCRATGATGTTAGCTG	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63954_Pvir_Dia2013_COI	BUNA7	1	BC63954_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	CTTAGTGGCATTAATTCGDAATCC	115.66124
BC63954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32272	WGS84	BC63954	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63954_Rhar_Sim2018_COI	BUNA7	1	BC63954_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63954	Sample	BC63954	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA7	COI	45	BC63954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA7	CTGCTGCTATTGCTCATG	115.66124
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63956_Aamu_Bax2006_COI	BUNA5	1	BC63956_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Aamu_Bax2006_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	GCACAACCGGGATCTTTACTTC	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63956_Pcan_Bot2011_IGS	BUNA5	1	BC63956_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Pcan_Bot2011_IGS	BUNA5	IGS	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	CGTAATCCTCAGTACTGGCTA	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63956_Pper_Dia2013_COI	BUNA5	1	BC63956_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Pper_Dia2013_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	CTTAGTGGCATTAATTCGDAATCC	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63956_Sspa_Oph2007_COI	BUNA5	1	BC63956_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Sspa_Oph2007_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	GAAGGCCTTCCGATGATC	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63956_Upin_Bot2011_COI	BUNA5	1	BC63956_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Upin_Bot2011_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	CTTTAATTACAGCGTTTTTATTGTTGT	115.66727
BC63956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32477	WGS84	BC63956	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BC63956_Vgib_Wil2025_COI	BUNA5	1	BC63956_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63956	Sample	BC63956	targeted	Absent	Sample	Vgib_Wil2025_COI	BUNA5	COI	45	BC63956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA5	CCCCTCTTTACAAGTTCTTTAGGGT	115.66727
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63957_Asen_Wil2025_COI	BUNA4	1	BC63957_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	GTTGTTGTTACAACACATGCTCTTTTG	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63957_Cjap_Sim2018_COI	BUNA4	1	BC63957_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63957_Esin_And2018_Cyb	BUNA4	1	BC63957_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA4	Cytb	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	ACCCCTCCTCATATCCAACCA	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63957_Mgig_Bot2012_COI	BUNA4	1	BC63957_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	TCTTATTCGTTGGAGACTTTATAACCCT	115.66539
BC63957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32441	WGS84	BC63957	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63957_Mjap_Gib2022_COI	BUNA4	1	BC63957_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63957	Sample	BC63957	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA4	COI	45	BC63957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA4	AATTTAATTGTGACTAGGCATGGTCTG	115.66539
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63958_Cmae_Bot2011_COI	BUNA3	1	BC63958_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Cmae_Bot2011_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	ATGAACAGTCTATCCTCCTTTAG	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BC63958_Hsan_Wil2021_COI	BUNA3	1	BC63958_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Hsan_Wil2021_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	CCTGGGCCGGTATAGTAGGT	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BC63958_Mros_Gib2022_COI	BUNA3	1	BC63958_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Mros_Gib2022_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	ACTTCTACCTCCTGCTCTTCTACTTCTC	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63958_Msal_Bot2012_COI	BUNA3	1	BC63958_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Msal_Bot2012_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	GYTAGTTCCRATGATGTTAGCTG	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63958_Pvir_Dia2013_COI	BUNA3	1	BC63958_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Pvir_Dia2013_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	CTTAGTGGCATTAATTCGDAATCC	115.66343
BC63958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32391	WGS84	BC63958	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63958_Rhar_Sim2018_COI	BUNA3	1	BC63958_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63958	Sample	BC63958	targeted	Absent	Sample	Rhar_Sim2018_COI	BUNA3	COI	45	BC63958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA3	CTGCTGCTATTGCTCATG	115.66343
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BC63960_Asen_Wil2025_COI	BUNA1	1	BC63960_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Asen_Wil2025_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	GTTGTTGTTACAACACATGCTCTTTTG	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63960_Cjap_Sim2018_COI	BUNA1	1	BC63960_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Cjap_Sim2018_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	TTAATATACGGTCATTTGGTATGAGTATAGATC	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BC63960_Esin_And2018_Cyb	BUNA1	1	BC63960_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Esin_And2018_Cyb	BUNA1	Cytb	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	ACCCCTCCTCATATCCAACCA	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63960_Mgig_Bot2012_COI	BUNA1	1	BC63960_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Mgig_Bot2012_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	TCTTATTCGTTGGAGACTTTATAACCCT	115.66645
BC63960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12953	-33.32686	WGS84	BC63960	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2020_Molecular_surveys-Bunbury_Summer	26/01/2020	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BC63960_Mjap_Gib2022_COI	BUNA1	1	BC63960_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63960	Sample	BC63960	targeted	Absent	Sample	Mjap_Gib2022_COI	BUNA1	COI	45	BC63960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	BUNA1	AATTTAATTGTGACTAGGCATGGTCTG	115.66645
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63961_Cmae_Bot2011_COI	GERA15	1	BC63961_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	ATGAACAGTCTATCCTCCTTTAG	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63961_Msal_Bot2012_COI	GERA15	1	BC63961_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Msal_Bot2012_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	GYTAGTTCCRATGATGTTAGCTG	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63961_Pvir_Dia2013_COI	GERA15	1	BC63961_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	CTTAGTGGCATTAATTCGDAATCC	114.59997
BC63961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77147	WGS84	BC63961	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63961_Rhar_Sim2018_COI	GERA15	1	BC63961_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63961	Sample	BC63961	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA15	COI	45	BC63961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA15	CTGCTGCTATTGCTCATG	114.59997
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63963_Aamu_Bax2006_COI	GERA13	1	BC63963_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	GCACAACCGGGATCTTTACTTC	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63963_Pcan_Bot2011_IGS	GERA13	1	BC63963_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA13	IGS	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	CGTAATCCTCAGTACTGGCTA	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63963_Pper_Dia2013_COI	GERA13	1	BC63963_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Pper_Dia2013_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	CTTAGTGGCATTAATTCGDAATCC	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63963_Sspa_Oph2007_COI	GERA13	1	BC63963_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	GAAGGCCTTCCGATGATC	114.5987
BC63963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77256	WGS84	BC63963	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63963_Upin_Bot2011_COI	GERA13	1	BC63963_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63963	Sample	BC63963	targeted	Absent	Sample	Upin_Bot2011_COI	GERA13	COI	45	BC63963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA13	CTTTAATTACAGCGTTTTTATTGTTGT	114.5987
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63964_Cjap_Sim2018_COI	GERA12	1	BC63964_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59739
BC63964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77269	WGS84	BC63964	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63964_Mgig_Bot2012_COI	GERA12	1	BC63964_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63964	Sample	BC63964	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA12	COI	45	BC63964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA12	TCTTATTCGTTGGAGACTTTATAACCCT	114.59739
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63965_Cmae_Bot2011_COI	GERA11	1	BC63965_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	ATGAACAGTCTATCCTCCTTTAG	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63965_Msal_Bot2012_COI	GERA11	1	BC63965_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Msal_Bot2012_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	GYTAGTTCCRATGATGTTAGCTG	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63965_Pvir_Dia2013_COI	GERA11	1	BC63965_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	CTTAGTGGCATTAATTCGDAATCC	114.59609
BC63965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77252	WGS84	BC63965	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63965_Rhar_Sim2018_COI	GERA11	1	BC63965_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63965	Sample	BC63965	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA11	COI	45	BC63965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA11	CTGCTGCTATTGCTCATG	114.59609
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63967_Aamu_Bax2006_COI	GERA9	1	BC63967_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	GCACAACCGGGATCTTTACTTC	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63967_Pcan_Bot2011_IGS	GERA9	1	BC63967_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA9	IGS	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	CGTAATCCTCAGTACTGGCTA	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63967_Pper_Dia2013_COI	GERA9	1	BC63967_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Pper_Dia2013_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	CTTAGTGGCATTAATTCGDAATCC	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63967_Sspa_Oph2007_COI	GERA9	1	BC63967_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	GAAGGCCTTCCGATGATC	114.5995
BC63967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77394	WGS84	BC63967	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63967_Upin_Bot2011_COI	GERA9	1	BC63967_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63967	Sample	BC63967	targeted	Absent	Sample	Upin_Bot2011_COI	GERA9	COI	45	BC63967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA9	CTTTAATTACAGCGTTTTTATTGTTGT	114.5995
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63968_Cjap_Sim2018_COI	GERA8	1	BC63968_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59813
BC63968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77367	WGS84	BC63968	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63968_Mgig_Bot2012_COI	GERA8	1	BC63968_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63968	Sample	BC63968	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA8	COI	45	BC63968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA8	TCTTATTCGTTGGAGACTTTATAACCCT	114.59813
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63969_Cmae_Bot2011_COI	GERA7	1	BC63969_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	ATGAACAGTCTATCCTCCTTTAG	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63969_Msal_Bot2012_COI	GERA7	1	BC63969_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Msal_Bot2012_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	GYTAGTTCCRATGATGTTAGCTG	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63969_Pvir_Dia2013_COI	GERA7	1	BC63969_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	CTTAGTGGCATTAATTCGDAATCC	114.59669
BC63969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.7735	WGS84	BC63969	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63969_Rhar_Sim2018_COI	GERA7	1	BC63969_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63969	Sample	BC63969	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA7	COI	45	BC63969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA7	CTGCTGCTATTGCTCATG	114.59669
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63970_Aamu_Bax2006_COI	GERA6	1	BC63970_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	GCACAACCGGGATCTTTACTTC	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63970_Pcan_Bot2011_IGS	GERA6	1	BC63970_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA6	IGS	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	CGTAATCCTCAGTACTGGCTA	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63970_Pper_Dia2013_COI	GERA6	1	BC63970_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Pper_Dia2013_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	CTTAGTGGCATTAATTCGDAATCC	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63970_Sspa_Oph2007_COI	GERA6	1	BC63970_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	GAAGGCCTTCCGATGATC	114.6002
BC63970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77502	WGS84	BC63970	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63970_Upin_Bot2011_COI	GERA6	1	BC63970_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63970	Sample	BC63970	targeted	Absent	Sample	Upin_Bot2011_COI	GERA6	COI	45	BC63970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA6	CTTTAATTACAGCGTTTTTATTGTTGT	114.6002
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63971_Cjap_Sim2018_COI	GERA5	1	BC63971_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59862
BC63971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63971	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63971_Mgig_Bot2012_COI	GERA5	1	BC63971_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63971	Sample	BC63971	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA5	COI	45	BC63971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA5	TCTTATTCGTTGGAGACTTTATAACCCT	114.59862
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC63972_Cmae_Bot2011_COI	GERA4	1	BC63972_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Cmae_Bot2011_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	ATGAACAGTCTATCCTCCTTTAG	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BC63972_Msal_Bot2012_COI	GERA4	1	BC63972_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Msal_Bot2012_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	GYTAGTTCCRATGATGTTAGCTG	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BC63972_Pvir_Dia2013_COI	GERA4	1	BC63972_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Pvir_Dia2013_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	CTTAGTGGCATTAATTCGDAATCC	114.59705
BC63972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77481	WGS84	BC63972	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BC63972_Rhar_Sim2018_COI	GERA4	1	BC63972_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63972	Sample	BC63972	targeted	Absent	Sample	Rhar_Sim2018_COI	GERA4	COI	45	BC63972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA4	CTGCTGCTATTGCTCATG	114.59705
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC63974_Aamu_Bax2006_COI	GERA2	1	BC63974_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Aamu_Bax2006_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	GCACAACCGGGATCTTTACTTC	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC63974_Pcan_Bot2011_IGS	GERA2	1	BC63974_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERA2	IGS	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	CGTAATCCTCAGTACTGGCTA	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC63974_Pper_Dia2013_COI	GERA2	1	BC63974_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Pper_Dia2013_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	CTTAGTGGCATTAATTCGDAATCC	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC63974_Sspa_Oph2007_COI	GERA2	1	BC63974_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Sspa_Oph2007_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	GAAGGCCTTCCGATGATC	114.59598
BC63974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77594	WGS84	BC63974	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC63974_Upin_Bot2011_COI	GERA2	1	BC63974_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63974	Sample	BC63974	targeted	Absent	Sample	Upin_Bot2011_COI	GERA2	COI	45	BC63974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA2	CTTTAATTACAGCGTTTTTATTGTTGT	114.59598
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC63975_Cjap_Sim2018_COI	GERA1	1	BC63975_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Cjap_Sim2018_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	TTAATATACGGTCATTTGGTATGAGTATAGATC	114.59449
BC63975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12954	-28.77552	WGS84	BC63975	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Summer	25/01/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC63975_Mgig_Bot2012_COI	GERA1	1	BC63975_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC63975	Sample	BC63975	targeted	Absent	Sample	Mgig_Bot2012_COI	GERA1	COI	45	BC63975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERA1	TCTTATTCGTTGGAGACTTTATAACCCT	114.59449
BC64564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81059	WGS84	BC64564	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64564_Aamu_Bax2006_COI	ADEAPC-60	1	BC64564_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64564	Sample	BC64564	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-60	COI	45	BC64564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-60	GCACAACCGGGATCTTTACTTC	138.54369
BC64564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81059	WGS84	BC64564	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64564_Pcan_Bot2011_IGS	ADEAPC-60	1	BC64564_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64564	Sample	BC64564	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-60	IGS	45	BC64564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-60	CGTAATCCTCAGTACTGGCTA	138.54369
BC64564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81059	WGS84	BC64564	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.495	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64564_Sspa_Oph2007_COI	ADEAPC-60	1	BC64564_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64564	Sample	BC64564	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-60	COI	45	BC64564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-60	GAAGGCCTTCCGATGATC	138.54369
BC64564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81059	WGS84	BC64564	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64564_Upin_Bot2011_COI	ADEAPC-60	1	BC64564_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64564	Sample	BC64564	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-60	COI	45	BC64564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-60	CTTTAATTACAGCGTTTTTATTGTTGT	138.54369
BC64565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8123	WGS84	BC64565	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64565_Cjap_Sim2018_COI	ADEAPC-59	1	BC64565_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64565	Sample	BC64565	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-59	COI	45	BC64565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-59	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.53897
BC64565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8123	WGS84	BC64565	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.677	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64565_Mgig_Bot2012_COI	ADEAPC-59	1	BC64565_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64565	Sample	BC64565	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-59	COI	45	BC64565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-59	TCTTATTCGTTGGAGACTTTATAACCCT	138.53897
BC64566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81333	WGS84	BC64566	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64566_Cmae_Bot2011_COI	ADEAPC-58	1	BC64566_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64566	Sample	BC64566	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-58	COI	45	BC64566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-58	ATGAACAGTCTATCCTCCTTTAG	138.53131
BC64568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81501	WGS84	BC64568	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64568_Aamu_Bax2006_COI	ADEAPC-56	1	BC64568_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64568	Sample	BC64568	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-56	COI	45	BC64568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-56	GCACAACCGGGATCTTTACTTC	138.51475
BC64568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81501	WGS84	BC64568	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64568_Pcan_Bot2011_IGS	ADEAPC-56	1	BC64568_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64568	Sample	BC64568	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-56	IGS	45	BC64568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-56	CGTAATCCTCAGTACTGGCTA	138.51475
BC64568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81501	WGS84	BC64568	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.681	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64568_Sspa_Oph2007_COI	ADEAPC-56	1	BC64568_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64568	Sample	BC64568	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-56	COI	45	BC64568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-56	GAAGGCCTTCCGATGATC	138.51475
BC64568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81501	WGS84	BC64568	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64568_Upin_Bot2011_COI	ADEAPC-56	1	BC64568_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64568	Sample	BC64568	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-56	COI	45	BC64568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-56	CTTTAATTACAGCGTTTTTATTGTTGT	138.51475
BC64569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81915	WGS84	BC64569	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64569_Cjap_Sim2018_COI	ADEAPC-55	1	BC64569_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64569	Sample	BC64569	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-55	COI	45	BC64569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-55	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51385
BC64569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81915	WGS84	BC64569	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.067	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64569_Mgig_Bot2012_COI	ADEAPC-55	1	BC64569_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64569	Sample	BC64569	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-55	COI	45	BC64569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-55	TCTTATTCGTTGGAGACTTTATAACCCT	138.51385
BC64571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83047	WGS84	BC64571	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64571_Aamu_Bax2006_COI	ADEAPC-53	1	BC64571_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64571	Sample	BC64571	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-53	COI	45	BC64571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-53	GCACAACCGGGATCTTTACTTC	138.51076
BC64571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83047	WGS84	BC64571	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64571_Pcan_Bot2011_IGS	ADEAPC-53	1	BC64571_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64571	Sample	BC64571	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-53	IGS	45	BC64571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-53	CGTAATCCTCAGTACTGGCTA	138.51076
BC64571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83047	WGS84	BC64571	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.548	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64571_Sspa_Oph2007_COI	ADEAPC-53	1	BC64571_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64571	Sample	BC64571	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-53	COI	45	BC64571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-53	GAAGGCCTTCCGATGATC	138.51076
BC64571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83047	WGS84	BC64571	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64571_Upin_Bot2011_COI	ADEAPC-53	1	BC64571_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64571	Sample	BC64571	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-53	COI	45	BC64571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-53	CTTTAATTACAGCGTTTTTATTGTTGT	138.51076
BC64572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83034	WGS84	BC64572	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64572_Cjap_Sim2018_COI	ADEAPC-52	1	BC64572_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64572	Sample	BC64572	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-52	COI	45	BC64572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-52	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51084
BC64572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83034	WGS84	BC64572	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.334	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64572_Mgig_Bot2012_COI	ADEAPC-52	1	BC64572_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64572	Sample	BC64572	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-52	COI	45	BC64572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-52	TCTTATTCGTTGGAGACTTTATAACCCT	138.51084
BC64573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83073	WGS84	BC64573	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64573_Cmae_Bot2011_COI	ADEAPC-51	1	BC64573_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64573	Sample	BC64573	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-51	COI	45	BC64573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-51	ATGAACAGTCTATCCTCCTTTAG	138.51131
BC64575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83738	WGS84	BC64575	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64575_Aamu_Bax2006_COI	ADEAPC-49	1	BC64575_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64575	Sample	BC64575	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-49	COI	45	BC64575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-49	GCACAACCGGGATCTTTACTTC	138.51065
BC64575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83738	WGS84	BC64575	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64575_Pcan_Bot2011_IGS	ADEAPC-49	1	BC64575_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64575	Sample	BC64575	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-49	IGS	45	BC64575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-49	CGTAATCCTCAGTACTGGCTA	138.51065
BC64575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83738	WGS84	BC64575	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.603	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64575_Sspa_Oph2007_COI	ADEAPC-49	1	BC64575_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64575	Sample	BC64575	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-49	COI	45	BC64575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-49	GAAGGCCTTCCGATGATC	138.51065
BC64575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83738	WGS84	BC64575	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64575_Upin_Bot2011_COI	ADEAPC-49	1	BC64575_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64575	Sample	BC64575	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-49	COI	45	BC64575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-49	CTTTAATTACAGCGTTTTTATTGTTGT	138.51065
BC64576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83724	WGS84	BC64576	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64576_Cjap_Sim2018_COI	ADEAPC-48	1	BC64576_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64576	Sample	BC64576	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-48	COI	45	BC64576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-48	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51052
BC64576	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83724	WGS84	BC64576	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.265	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64576_Mgig_Bot2012_COI	ADEAPC-48	1	BC64576_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64576	Sample	BC64576	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-48	COI	45	BC64576	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-48	TCTTATTCGTTGGAGACTTTATAACCCT	138.51052
BC64577	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.83723	WGS84	BC64577	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64577_Cmae_Bot2011_COI	ADEAPC-47	1	BC64577_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64577	Sample	BC64577	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-47	COI	45	BC64577	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-47	ATGAACAGTCTATCCTCCTTTAG	138.5104
BC64579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80372	WGS84	BC64579	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64579_Aamu_Bax2006_COI	ADEAPC-45	1	BC64579_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64579	Sample	BC64579	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-45	COI	45	BC64579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-45	GCACAACCGGGATCTTTACTTC	138.54048
BC64579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80372	WGS84	BC64579	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64579_Pcan_Bot2011_IGS	ADEAPC-45	1	BC64579_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64579	Sample	BC64579	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-45	IGS	45	BC64579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-45	CGTAATCCTCAGTACTGGCTA	138.54048
BC64579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80372	WGS84	BC64579	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.477	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64579_Sspa_Oph2007_COI	ADEAPC-45	1	BC64579_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64579	Sample	BC64579	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-45	COI	45	BC64579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-45	GAAGGCCTTCCGATGATC	138.54048
BC64579	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80372	WGS84	BC64579	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64579_Upin_Bot2011_COI	ADEAPC-45	1	BC64579_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64579	Sample	BC64579	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-45	COI	45	BC64579	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-45	CTTTAATTACAGCGTTTTTATTGTTGT	138.54048
BC64580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80499	WGS84	BC64580	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64580_Cmae_Bot2011_COI	ADEAPC-44	1	BC64580_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64580	Sample	BC64580	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-44	COI	45	BC64580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-44	ATGAACAGTCTATCCTCCTTTAG	138.54584
BC64582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8005	WGS84	BC64582	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64582_Aamu_Bax2006_COI	ADEAPC-42	1	BC64582_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64582	Sample	BC64582	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-42	COI	45	BC64582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-42	GCACAACCGGGATCTTTACTTC	138.54783
BC64582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8005	WGS84	BC64582	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64582_Pcan_Bot2011_IGS	ADEAPC-42	1	BC64582_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64582	Sample	BC64582	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-42	IGS	45	BC64582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-42	CGTAATCCTCAGTACTGGCTA	138.54783
BC64582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8005	WGS84	BC64582	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	31.508	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64582_Sspa_Oph2007_COI	ADEAPC-42	1	BC64582_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64582	Sample	BC64582	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-42	COI	45	BC64582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-42	GAAGGCCTTCCGATGATC	138.54783
BC64582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.8005	WGS84	BC64582	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64582_Upin_Bot2011_COI	ADEAPC-42	1	BC64582_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64582	Sample	BC64582	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-42	COI	45	BC64582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-42	CTTTAATTACAGCGTTTTTATTGTTGT	138.54783
BC64583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79895	WGS84	BC64583	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64583_Cjap_Sim2018_COI	ADEAPC-41	1	BC64583_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64583	Sample	BC64583	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-41	COI	45	BC64583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-41	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54817
BC64583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79895	WGS84	BC64583	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.872	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64583_Mgig_Bot2012_COI	ADEAPC-41	1	BC64583_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64583	Sample	BC64583	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-41	COI	45	BC64583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-41	TCTTATTCGTTGGAGACTTTATAACCCT	138.54817
BC64584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79447	WGS84	BC64584	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64584_Cmae_Bot2011_COI	ADEAPC-40	1	BC64584_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64584	Sample	BC64584	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-40	COI	45	BC64584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-40	ATGAACAGTCTATCCTCCTTTAG	138.54594
BC64586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78712	WGS84	BC64586	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64586_Aamu_Bax2006_COI	ADEAPC-38	1	BC64586_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64586	Sample	BC64586	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-38	COI	45	BC64586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-38	GCACAACCGGGATCTTTACTTC	138.54309
BC64586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78712	WGS84	BC64586	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64586_Pcan_Bot2011_IGS	ADEAPC-38	1	BC64586_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64586	Sample	BC64586	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-38	IGS	45	BC64586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-38	CGTAATCCTCAGTACTGGCTA	138.54309
BC64586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78712	WGS84	BC64586	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64586_Sspa_Oph2007_COI	ADEAPC-38	1	BC64586_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64586	Sample	BC64586	targeted	Absent	Sample	Sspa_Oph2007_COI	ADEAPC-38	COI	45	BC64586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-38	GAAGGCCTTCCGATGATC	138.54309
BC64586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78712	WGS84	BC64586	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64586_Upin_Bot2011_COI	ADEAPC-38	1	BC64586_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64586	Sample	BC64586	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-38	COI	45	BC64586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-38	CTTTAATTACAGCGTTTTTATTGTTGT	138.54309
BC64587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78384	WGS84	BC64587	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64587_Cjap_Sim2018_COI	ADEAPC-37	1	BC64587_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64587	Sample	BC64587	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-37	COI	45	BC64587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-37	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.54032
BC64587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78384	WGS84	BC64587	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.801	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64587_Mgig_Bot2012_COI	ADEAPC-37	1	BC64587_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64587	Sample	BC64587	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-37	COI	45	BC64587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-37	TCTTATTCGTTGGAGACTTTATAACCCT	138.54032
BC64588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78076	WGS84	BC64588	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64588_Cmae_Bot2011_COI	ADEAPC-36	1	BC64588_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64588	Sample	BC64588	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-36	COI	45	BC64588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-36	ATGAACAGTCTATCCTCCTTTAG	138.5374
BC64590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77469	WGS84	BC64590	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64590_Cjap_Sim2018_COI	ADEAPC-34	1	BC64590_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64590	Sample	BC64590	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-34	COI	45	BC64590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-34	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.53169
BC64590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77469	WGS84	BC64590	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.339	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64590_Mgig_Bot2012_COI	ADEAPC-34	1	BC64590_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64590	Sample	BC64590	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-34	COI	45	BC64590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-34	TCTTATTCGTTGGAGACTTTATAACCCT	138.53169
BC64591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77128	WGS84	BC64591	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64591_Cmae_Bot2011_COI	ADEAPC-33	1	BC64591_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64591	Sample	BC64591	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-33	COI	45	BC64591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-33	ATGAACAGTCTATCCTCCTTTAG	138.52947
BC64593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76424	WGS84	BC64593	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64593_Aamu_Bax2006_COI	ADEAPC-31	1	BC64593_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64593	Sample	BC64593	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-31	COI	45	BC64593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-31	GCACAACCGGGATCTTTACTTC	138.52591
BC64593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76424	WGS84	BC64593	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64593_Pcan_Bot2011_IGS	ADEAPC-31	1	BC64593_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64593	Sample	BC64593	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-31	IGS	45	BC64593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-31	CGTAATCCTCAGTACTGGCTA	138.52591
BC64593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76424	WGS84	BC64593	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64593_Sspa_Oph2007_COI	ADEAPC-31	1	BC64593_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64593	Sample	BC64593	targeted	Absent	Sample	Sspa_Oph2007_COI	ADEAPC-31	COI	45	BC64593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-31	GAAGGCCTTCCGATGATC	138.52591
BC64593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76424	WGS84	BC64593	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64593_Upin_Bot2011_COI	ADEAPC-31	1	BC64593_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64593	Sample	BC64593	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-31	COI	45	BC64593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-31	CTTTAATTACAGCGTTTTTATTGTTGT	138.52591
BC64594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76096	WGS84	BC64594	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64594_Cjap_Sim2018_COI	ADEAPC-30	1	BC64594_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64594	Sample	BC64594	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-30	COI	45	BC64594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-30	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52349
BC64594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76096	WGS84	BC64594	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.275	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64594_Mgig_Bot2012_COI	ADEAPC-30	1	BC64594_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64594	Sample	BC64594	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-30	COI	45	BC64594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-30	TCTTATTCGTTGGAGACTTTATAACCCT	138.52349
BC64595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.75796	WGS84	BC64595	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	18/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64595_Cmae_Bot2011_COI	ADEAPC-29	1	BC64595_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64595	Sample	BC64595	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-29	COI	45	BC64595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-29	ATGAACAGTCTATCCTCCTTTAG	138.52051
BC64597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81029	WGS84	BC64597	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64597_Aamu_Bax2006_COI	ADEAPC-27	1	BC64597_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64597	Sample	BC64597	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-27	COI	45	BC64597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-27	GCACAACCGGGATCTTTACTTC	138.52168
BC64597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81029	WGS84	BC64597	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64597_Pcan_Bot2011_IGS	ADEAPC-27	1	BC64597_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64597	Sample	BC64597	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-27	IGS	45	BC64597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-27	CGTAATCCTCAGTACTGGCTA	138.52168
BC64597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81029	WGS84	BC64597	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.286	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64597_Sspa_Oph2007_COI	ADEAPC-27	1	BC64597_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64597	Sample	BC64597	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-27	COI	45	BC64597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-27	GAAGGCCTTCCGATGATC	138.52168
BC64597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.81029	WGS84	BC64597	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64597_Upin_Bot2011_COI	ADEAPC-27	1	BC64597_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64597	Sample	BC64597	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-27	COI	45	BC64597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-27	CTTTAATTACAGCGTTTTTATTGTTGT	138.52168
BC64598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80908	WGS84	BC64598	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64598_Cjap_Sim2018_COI	ADEAPC-26	1	BC64598_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64598	Sample	BC64598	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-26	COI	45	BC64598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-26	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51913
BC64598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80908	WGS84	BC64598	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.891	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64598_Mgig_Bot2012_COI	ADEAPC-26	1	BC64598_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64598	Sample	BC64598	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-26	COI	45	BC64598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-26	TCTTATTCGTTGGAGACTTTATAACCCT	138.51913
BC64599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80869	WGS84	BC64599	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64599_Cmae_Bot2011_COI	ADEAPC-25	1	BC64599_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64599	Sample	BC64599	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-25	COI	45	BC64599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-25	ATGAACAGTCTATCCTCCTTTAG	138.5177
BC64601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80038	WGS84	BC64601	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64601_Aamu_Bax2006_COI	ADEAPC-23	1	BC64601_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64601	Sample	BC64601	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-23	COI	45	BC64601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-23	GCACAACCGGGATCTTTACTTC	138.51358
BC64601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80038	WGS84	BC64601	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64601_Pcan_Bot2011_IGS	ADEAPC-23	1	BC64601_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64601	Sample	BC64601	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-23	IGS	45	BC64601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-23	CGTAATCCTCAGTACTGGCTA	138.51358
BC64601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80038	WGS84	BC64601	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.54	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64601_Sspa_Oph2007_COI	ADEAPC-23	1	BC64601_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64601	Sample	BC64601	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-23	COI	45	BC64601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-23	GAAGGCCTTCCGATGATC	138.51358
BC64601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.80038	WGS84	BC64601	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64601_Upin_Bot2011_COI	ADEAPC-23	1	BC64601_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64601	Sample	BC64601	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-23	COI	45	BC64601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-23	CTTTAATTACAGCGTTTTTATTGTTGT	138.51358
BC64602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79792	WGS84	BC64602	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64602_Cjap_Sim2018_COI	ADEAPC-22	1	BC64602_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64602	Sample	BC64602	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-22	COI	45	BC64602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-22	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51337
BC64602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79792	WGS84	BC64602	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.621	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64602_Mgig_Bot2012_COI	ADEAPC-22	1	BC64602_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64602	Sample	BC64602	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-22	COI	45	BC64602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-22	TCTTATTCGTTGGAGACTTTATAACCCT	138.51337
BC64603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.79528	WGS84	BC64603	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64603_Cmae_Bot2011_COI	ADEAPC-21	1	BC64603_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64603	Sample	BC64603	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-21	COI	45	BC64603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-21	ATGAACAGTCTATCCTCCTTTAG	138.51364
BC64605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78832	WGS84	BC64605	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64605_Aamu_Bax2006_COI	ADEAPC-19	1	BC64605_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64605	Sample	BC64605	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-19	COI	45	BC64605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-19	GCACAACCGGGATCTTTACTTC	138.51547
BC64605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78832	WGS84	BC64605	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64605_Pcan_Bot2011_IGS	ADEAPC-19	1	BC64605_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64605	Sample	BC64605	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-19	IGS	45	BC64605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-19	CGTAATCCTCAGTACTGGCTA	138.51547
BC64605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78832	WGS84	BC64605	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.577	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64605_Sspa_Oph2007_COI	ADEAPC-19	1	BC64605_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64605	Sample	BC64605	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-19	COI	45	BC64605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-19	GAAGGCCTTCCGATGATC	138.51547
BC64605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78832	WGS84	BC64605	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64605_Upin_Bot2011_COI	ADEAPC-19	1	BC64605_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64605	Sample	BC64605	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-19	COI	45	BC64605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.51547
BC64606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78608	WGS84	BC64606	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64606_Cjap_Sim2018_COI	ADEAPC-18	1	BC64606_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64606	Sample	BC64606	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-18	COI	45	BC64606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-18	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51602
BC64606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78608	WGS84	BC64606	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.356	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64606_Mgig_Bot2012_COI	ADEAPC-18	1	BC64606_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64606	Sample	BC64606	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-18	COI	45	BC64606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-18	TCTTATTCGTTGGAGACTTTATAACCCT	138.51602
BC64607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.78364	WGS84	BC64607	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64607_Cmae_Bot2011_COI	ADEAPC-17	1	BC64607_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64607	Sample	BC64607	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-17	COI	45	BC64607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-17	ATGAACAGTCTATCCTCCTTTAG	138.51668
BC64609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.778	WGS84	BC64609	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64609_Aamu_Bax2006_COI	ADEAPC-15	1	BC64609_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64609	Sample	BC64609	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-15	COI	45	BC64609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-15	GCACAACCGGGATCTTTACTTC	138.51913
BC64609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.778	WGS84	BC64609	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64609_Pcan_Bot2011_IGS	ADEAPC-15	1	BC64609_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64609	Sample	BC64609	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-15	IGS	45	BC64609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-15	CGTAATCCTCAGTACTGGCTA	138.51913
BC64609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.778	WGS84	BC64609	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	29.398	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64609_Sspa_Oph2007_COI	ADEAPC-15	1	BC64609_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64609	Sample	BC64609	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-15	COI	45	BC64609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-15	GAAGGCCTTCCGATGATC	138.51913
BC64609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.778	WGS84	BC64609	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64609_Upin_Bot2011_COI	ADEAPC-15	1	BC64609_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64609	Sample	BC64609	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-15	COI	45	BC64609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-15	CTTTAATTACAGCGTTTTTATTGTTGT	138.51913
BC64610	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77268	WGS84	BC64610	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64610_Cmae_Bot2011_COI	ADEAPC-14	1	BC64610_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64610	Sample	BC64610	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-14	COI	45	BC64610	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-14	ATGAACAGTCTATCCTCCTTTAG	138.51969
BC64612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76536	WGS84	BC64612	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64612_Aamu_Bax2006_COI	ADEAPC-12	1	BC64612_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64612	Sample	BC64612	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-12	COI	45	BC64612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-12	GCACAACCGGGATCTTTACTTC	138.51727
BC64612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76536	WGS84	BC64612	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64612_Pcan_Bot2011_IGS	ADEAPC-12	1	BC64612_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64612	Sample	BC64612	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-12	IGS	45	BC64612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-12	CGTAATCCTCAGTACTGGCTA	138.51727
BC64612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76536	WGS84	BC64612	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.538	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64612_Sspa_Oph2007_COI	ADEAPC-12	1	BC64612_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64612	Sample	BC64612	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-12	COI	45	BC64612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-12	GAAGGCCTTCCGATGATC	138.51727
BC64612	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76536	WGS84	BC64612	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64612_Upin_Bot2011_COI	ADEAPC-12	1	BC64612_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64612	Sample	BC64612	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-12	COI	45	BC64612	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.51727
BC64613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76148	WGS84	BC64613	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64613_Cjap_Sim2018_COI	ADEAPC-11	1	BC64613_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64613	Sample	BC64613	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-11	COI	45	BC64613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51153
BC64613	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76148	WGS84	BC64613	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.706	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64613_Mgig_Bot2012_COI	ADEAPC-11	1	BC64613_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64613	Sample	BC64613	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-11	COI	45	BC64613	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.51153
BC64614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76032	WGS84	BC64614	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64614_Cmae_Bot2011_COI	ADEAPC-10	1	BC64614_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64614	Sample	BC64614	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-10	COI	45	BC64614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-10	ATGAACAGTCTATCCTCCTTTAG	138.50655
BC64616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76234	WGS84	BC64616	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64616_Aamu_Bax2006_COI	ADEAPC-08	1	BC64616_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64616	Sample	BC64616	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-08	COI	45	BC64616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-08	GCACAACCGGGATCTTTACTTC	138.49388
BC64616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76234	WGS84	BC64616	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64616_Pcan_Bot2011_IGS	ADEAPC-08	1	BC64616_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64616	Sample	BC64616	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-08	IGS	45	BC64616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-08	CGTAATCCTCAGTACTGGCTA	138.49388
BC64616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76234	WGS84	BC64616	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.044	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64616_Sspa_Oph2007_COI	ADEAPC-08	1	BC64616_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64616	Sample	BC64616	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-08	COI	45	BC64616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-08	GAAGGCCTTCCGATGATC	138.49388
BC64616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76234	WGS84	BC64616	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64616_Upin_Bot2011_COI	ADEAPC-08	1	BC64616_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64616	Sample	BC64616	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-08	COI	45	BC64616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.49388
BC64617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76304	WGS84	BC64617	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64617_Cjap_Sim2018_COI	ADEAPC-07	1	BC64617_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64617	Sample	BC64617	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-07	COI	45	BC64617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49257
BC64617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76304	WGS84	BC64617	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.698	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64617_Mgig_Bot2012_COI	ADEAPC-07	1	BC64617_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64617	Sample	BC64617	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-07	COI	45	BC64617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.49257
BC64618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76372	WGS84	BC64618	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64618_Cmae_Bot2011_COI	ADEAPC-06	1	BC64618_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64618	Sample	BC64618	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-06	COI	45	BC64618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-06	ATGAACAGTCTATCCTCCTTTAG	138.49151
BC64620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76871	WGS84	BC64620	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC64620_Cjap_Sim2018_COI	ADEAPC-04	1	BC64620_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64620	Sample	BC64620	targeted	Absent	Sample	Cjap_Sim2018_COI	ADEAPC-04	COI	45	BC64620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48329
BC64620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76871	WGS84	BC64620	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.789	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC64620_Mgig_Bot2012_COI	ADEAPC-04	1	BC64620_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64620	Sample	BC64620	targeted	Present	Sample	Mgig_Bot2012_COI	ADEAPC-04	COI	45	BC64620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.48329
BC64621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.76978	WGS84	BC64621	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC64621_Cmae_Bot2011_COI	ADEAPC-03	1	BC64621_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64621	Sample	BC64621	targeted	Absent	Sample	Cmae_Bot2011_COI	ADEAPC-03	COI	45	BC64621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-03	ATGAACAGTCTATCCTCCTTTAG	138.48298
BC64623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77103	WGS84	BC64623	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC64623_Aamu_Bax2006_COI	ADEAPC-01	1	BC64623_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64623	Sample	BC64623	targeted	Absent	Sample	Aamu_Bax2006_COI	ADEAPC-01	COI	45	BC64623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-01	GCACAACCGGGATCTTTACTTC	138.48203
BC64623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77103	WGS84	BC64623	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC64623_Pcan_Bot2011_IGS	ADEAPC-01	1	BC64623_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64623	Sample	BC64623	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADEAPC-01	IGS	45	BC64623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-01	CGTAATCCTCAGTACTGGCTA	138.48203
BC64623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77103	WGS84	BC64623	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.627	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC64623_Sspa_Oph2007_COI	ADEAPC-01	1	BC64623_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64623	Sample	BC64623	targeted	Present	Sample	Sspa_Oph2007_COI	ADEAPC-01	COI	45	BC64623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-01	GAAGGCCTTCCGATGATC	138.48203
BC64623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_12952	-34.77103	WGS84	BC64623	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Paddle_Crab_IMS_survey	17/03/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC64623_Upin_Bot2011_COI	ADEAPC-01	1	BC64623_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC64623	Sample	BC64623	targeted	Absent	Sample	Upin_Bot2011_COI	ADEAPC-01	COI	45	BC64623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADEAPC-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.48203
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC76569_Aamu_Bax2006_COI	GERB1	1	BC76569_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Aamu_Bax2006_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	GCACAACCGGGATCTTTACTTC	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC76569_Pcan_Bot2011_IGS	GERB1	1	BC76569_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Pcan_Bot2011_IGS	GERB1	IGS	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	CGTAATCCTCAGTACTGGCTA	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BC76569_Pper_Dia2013_COI	GERB1	1	BC76569_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Pper_Dia2013_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	CTTAGTGGCATTAATTCGDAATCC	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC76569_Sspa_Oph2007_COI	GERB1	1	BC76569_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Sspa_Oph2007_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	GAAGGCCTTCCGATGATC	114.59414
BC76569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13076	-28.77571	WGS84	BC76569	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveys for priority marine pests in  2020 for the ports of Bunbury, Kwinana, Fremantle and Geraldton. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000354-1. SARDI Research Report Series No. 1082, Adelaide.	2020_Molecular_surveys-Geraldton_Autumn	19/05/2020	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC76569_Upin_Bot2011_COI	GERB1	1	BC76569_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC76569	Sample	BC76569	targeted	Absent	Sample	Upin_Bot2011_COI	GERB1	COI	45	BC76569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GERB1	CTTTAATTACAGCGTTTTTATTGTTGT	114.59414
BC77251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84457	WGS84	BC77251	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77251_Cjap_Sim2018_COI	S5-04	1	BC77251_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77251	Sample	BC77251	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-04	COI	45	BC77251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49741
BC77251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84457	WGS84	BC77251	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.578	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77251_Mgig_Bot2012_COI	S5-04	1	BC77251_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77251	Sample	BC77251	targeted	Present	Sample	Mgig_Bot2012_COI	S5-04	COI	45	BC77251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.49741
BC77252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83697	WGS84	BC77252	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77252_Cmae_Bot2011_COI	S5-09	1	BC77252_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77252	Sample	BC77252	targeted	Absent	Sample	Cmae_Bot2011_COI	S5-09	COI	45	BC77252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-09	ATGAACAGTCTATCCTCCTTTAG	138.51049
BC77254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77376	WGS84	BC77254	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77254_Aamu_Bax2006_COI	S5-19	1	BC77254_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77254	Sample	BC77254	targeted	Absent	Sample	Aamu_Bax2006_COI	S5-19	COI	45	BC77254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-19	GCACAACCGGGATCTTTACTTC	138.48542
BC77254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77376	WGS84	BC77254	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77254_Pcan_Bot2011_IGS	S5-19	1	BC77254_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77254	Sample	BC77254	targeted	Absent	Sample	Pcan_Bot2011_IGS	S5-19	IGS	45	BC77254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-19	CGTAATCCTCAGTACTGGCTA	138.48542
BC77254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77376	WGS84	BC77254	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.105	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77254_Sspa_Oph2007_COI	S5-19	1	BC77254_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77254	Sample	BC77254	targeted	Present	Sample	Sspa_Oph2007_COI	S5-19	COI	45	BC77254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-19	GAAGGCCTTCCGATGATC	138.48542
BC77254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77376	WGS84	BC77254	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77254_Upin_Bot2011_COI	S5-19	1	BC77254_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77254	Sample	BC77254	targeted	Absent	Sample	Upin_Bot2011_COI	S5-19	COI	45	BC77254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.48542
BC77255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81058	WGS84	BC77255	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S05	26/03/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77255_Cjap_Sim2018_COI	S5-23	1	BC77255_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77255	Sample	BC77255	targeted	Absent	Sample	Cjap_Sim2018_COI	S5-23	COI	45	BC77255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-23	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52126
BC77255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81058	WGS84	BC77255	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.148	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S05	26/03/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77255_Mgig_Bot2012_COI	S5-23	1	BC77255_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77255	Sample	BC77255	targeted	Present	Sample	Mgig_Bot2012_COI	S5-23	COI	45	BC77255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S5-23	TCTTATTCGTTGGAGACTTTATAACCCT	138.52126
BC77256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84392	WGS84	BC77256	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	32.686	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77256_Cmae_Bot2011_COI	S6-04	1	BC77256_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77256	Sample	BC77256	targeted	Present	Sample	Cmae_Bot2011_COI	S6-04	COI	45	BC77256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-04	ATGAACAGTCTATCCTCCTTTAG	138.49698
BC77258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78399	WGS84	BC77258	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77258_Aamu_Bax2006_COI	S6-14	1	BC77258_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77258	Sample	BC77258	targeted	Absent	Sample	Aamu_Bax2006_COI	S6-14	COI	45	BC77258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-14	GCACAACCGGGATCTTTACTTC	138.51578
BC77258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78399	WGS84	BC77258	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77258_Pcan_Bot2011_IGS	S6-14	1	BC77258_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77258	Sample	BC77258	targeted	Absent	Sample	Pcan_Bot2011_IGS	S6-14	IGS	45	BC77258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-14	CGTAATCCTCAGTACTGGCTA	138.51578
BC77258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78399	WGS84	BC77258	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77258_Sspa_Oph2007_COI	S6-14	1	BC77258_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77258	Sample	BC77258	targeted	Absent	Sample	Sspa_Oph2007_COI	S6-14	COI	45	BC77258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-14	GAAGGCCTTCCGATGATC	138.51578
BC77258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78399	WGS84	BC77258	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77258_Upin_Bot2011_COI	S6-14	1	BC77258_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77258	Sample	BC77258	targeted	Absent	Sample	Upin_Bot2011_COI	S6-14	COI	45	BC77258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.51578
BC77259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77244	WGS84	BC77259	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S06	24/04/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77259_Cjap_Sim2018_COI	S6-19	1	BC77259_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77259	Sample	BC77259	targeted	Absent	Sample	Cjap_Sim2018_COI	S6-19	COI	45	BC77259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48557
BC77259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77244	WGS84	BC77259	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S06	24/04/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77259_Mgig_Bot2012_COI	S6-19	1	BC77259_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77259	Sample	BC77259	targeted	Absent	Sample	Mgig_Bot2012_COI	S6-19	COI	45	BC77259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S6-19	TCTTATTCGTTGGAGACTTTATAACCCT	138.48557
BC77261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84489	WGS84	BC77261	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77261_Aamu_Bax2006_COI	S7-04	1	BC77261_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77261	Sample	BC77261	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-04	COI	45	BC77261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-04	GCACAACCGGGATCTTTACTTC	138.49712
BC77261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84489	WGS84	BC77261	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77261_Pcan_Bot2011_IGS	S7-04	1	BC77261_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77261	Sample	BC77261	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-04	IGS	45	BC77261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-04	CGTAATCCTCAGTACTGGCTA	138.49712
BC77261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84489	WGS84	BC77261	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.43	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77261_Sspa_Oph2007_COI	S7-04	1	BC77261_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77261	Sample	BC77261	targeted	Present	Sample	Sspa_Oph2007_COI	S7-04	COI	45	BC77261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-04	GAAGGCCTTCCGATGATC	138.49712
BC77261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84489	WGS84	BC77261	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77261_Upin_Bot2011_COI	S7-04	1	BC77261_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77261	Sample	BC77261	targeted	Absent	Sample	Upin_Bot2011_COI	S7-04	COI	45	BC77261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-04	CTTTAATTACAGCGTTTTTATTGTTGT	138.49712
BC77262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83653	WGS84	BC77262	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77262_Cjap_Sim2018_COI	S7-09	1	BC77262_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77262	Sample	BC77262	targeted	Absent	Sample	Cjap_Sim2018_COI	S7-09	COI	45	BC77262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50914
BC77262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83653	WGS84	BC77262	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S07	30/05/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77262_Mgig_Bot2012_COI	S7-09	1	BC77262_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77262	Sample	BC77262	targeted	Absent	Sample	Mgig_Bot2012_COI	S7-09	COI	45	BC77262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.50914
BC77263	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78246	WGS84	BC77263	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77263_Cmae_Bot2011_COI	S7-14	1	BC77263_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77263	Sample	BC77263	targeted	Absent	Sample	Cmae_Bot2011_COI	S7-14	COI	45	BC77263	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-14	ATGAACAGTCTATCCTCCTTTAG	138.51648
BC77265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81053	WGS84	BC77265	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77265_Aamu_Bax2006_COI	S7-23	1	BC77265_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77265	Sample	BC77265	targeted	Absent	Sample	Aamu_Bax2006_COI	S7-23	COI	45	BC77265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-23	GCACAACCGGGATCTTTACTTC	138.52131
BC77265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81053	WGS84	BC77265	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77265_Pcan_Bot2011_IGS	S7-23	1	BC77265_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77265	Sample	BC77265	targeted	Absent	Sample	Pcan_Bot2011_IGS	S7-23	IGS	45	BC77265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-23	CGTAATCCTCAGTACTGGCTA	138.52131
BC77265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81053	WGS84	BC77265	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	30.479	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77265_Sspa_Oph2007_COI	S7-23	1	BC77265_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77265	Sample	BC77265	targeted	Present	Sample	Sspa_Oph2007_COI	S7-23	COI	45	BC77265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-23	GAAGGCCTTCCGATGATC	138.52131
BC77265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81053	WGS84	BC77265	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S07	30/05/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77265_Upin_Bot2011_COI	S7-23	1	BC77265_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77265	Sample	BC77265	targeted	Absent	Sample	Upin_Bot2011_COI	S7-23	COI	45	BC77265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S7-23	CTTTAATTACAGCGTTTTTATTGTTGT	138.52131
BC77266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81019	WGS84	BC77266	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77266_Cjap_Sim2018_COI	S8-04	1	BC77266_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77266	Sample	BC77266	targeted	Absent	Sample	Cjap_Sim2018_COI	S8-04	COI	45	BC77266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.52036
BC77266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81019	WGS84	BC77266	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S08	8/01/2018	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77266_Mgig_Bot2012_COI	S8-04	1	BC77266_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77266	Sample	BC77266	targeted	Absent	Sample	Mgig_Bot2012_COI	S8-04	COI	45	BC77266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.52036
BC77267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78552	WGS84	BC77267	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77267_Cmae_Bot2011_COI	S8-09	1	BC77267_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77267	Sample	BC77267	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-09	COI	45	BC77267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-09	ATGAACAGTCTATCCTCCTTTAG	138.51387
BC77269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84801	WGS84	BC77269	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77269_Aamu_Bax2006_COI	S8-19	1	BC77269_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77269	Sample	BC77269	targeted	Absent	Sample	Aamu_Bax2006_COI	S8-19	COI	45	BC77269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-19	GCACAACCGGGATCTTTACTTC	138.49777
BC77269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84801	WGS84	BC77269	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77269_Pcan_Bot2011_IGS	S8-19	1	BC77269_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77269	Sample	BC77269	targeted	Absent	Sample	Pcan_Bot2011_IGS	S8-19	IGS	45	BC77269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-19	CGTAATCCTCAGTACTGGCTA	138.49777
BC77269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84801	WGS84	BC77269	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.409	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77269_Sspa_Oph2007_COI	S8-19	1	BC77269_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77269	Sample	BC77269	targeted	Present	Sample	Sspa_Oph2007_COI	S8-19	COI	45	BC77269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-19	GAAGGCCTTCCGATGATC	138.49777
BC77269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84801	WGS84	BC77269	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77269_Upin_Bot2011_COI	S8-19	1	BC77269_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77269	Sample	BC77269	targeted	Absent	Sample	Upin_Bot2011_COI	S8-19	COI	45	BC77269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-19	CTTTAATTACAGCGTTTTTATTGTTGT	138.49777
BC77270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83692	WGS84	BC77270	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S08	8/01/2018	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77270_Cmae_Bot2011_COI	S8-23	1	BC77270_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77270	Sample	BC77270	targeted	Absent	Sample	Cmae_Bot2011_COI	S8-23	COI	45	BC77270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S8-23	ATGAACAGTCTATCCTCCTTTAG	138.50975
BC77272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8366	WGS84	BC77272	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77272_Aamu_Bax2006_COI	S16-02	1	BC77272_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77272	Sample	BC77272	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-02	COI	45	BC77272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-02	GCACAACCGGGATCTTTACTTC	138.50718
BC77272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8366	WGS84	BC77272	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77272_Pcan_Bot2011_IGS	S16-02	1	BC77272_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77272	Sample	BC77272	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-02	IGS	45	BC77272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-02	CGTAATCCTCAGTACTGGCTA	138.50718
BC77272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8366	WGS84	BC77272	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77272_Sspa_Oph2007_COI	S16-02	1	BC77272_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77272	Sample	BC77272	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-02	COI	45	BC77272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-02	GAAGGCCTTCCGATGATC	138.50718
BC77272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8366	WGS84	BC77272	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77272_Upin_Bot2011_COI	S16-02	1	BC77272_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77272	Sample	BC77272	targeted	Absent	Sample	Upin_Bot2011_COI	S16-02	COI	45	BC77272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-02	CTTTAATTACAGCGTTTTTATTGTTGT	138.50718
BC77273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83638	WGS84	BC77273	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77273_Cjap_Sim2018_COI	S16-03	1	BC77273_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77273	Sample	BC77273	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-03	COI	45	BC77273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50866
BC77273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83638	WGS84	BC77273	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.905	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77273_Mgig_Bot2012_COI	S16-03	1	BC77273_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77273	Sample	BC77273	targeted	Present	Sample	Mgig_Bot2012_COI	S16-03	COI	45	BC77273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-03	TCTTATTCGTTGGAGACTTTATAACCCT	138.50866
BC77274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83522	WGS84	BC77274	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77274_Cmae_Bot2011_COI	S16-04	1	BC77274_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77274	Sample	BC77274	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-04	COI	45	BC77274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-04	ATGAACAGTCTATCCTCCTTTAG	138.50734
BC77276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80831	WGS84	BC77276	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77276_Aamu_Bax2006_COI	S16-06	1	BC77276_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77276	Sample	BC77276	targeted	Absent	Sample	Aamu_Bax2006_COI	S16-06	COI	45	BC77276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-06	GCACAACCGGGATCTTTACTTC	138.516
BC77276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80831	WGS84	BC77276	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77276_Pcan_Bot2011_IGS	S16-06	1	BC77276_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77276	Sample	BC77276	targeted	Absent	Sample	Pcan_Bot2011_IGS	S16-06	IGS	45	BC77276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-06	CGTAATCCTCAGTACTGGCTA	138.516
BC77276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80831	WGS84	BC77276	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77276_Sspa_Oph2007_COI	S16-06	1	BC77276_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77276	Sample	BC77276	targeted	Absent	Sample	Sspa_Oph2007_COI	S16-06	COI	45	BC77276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-06	GAAGGCCTTCCGATGATC	138.516
BC77276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80831	WGS84	BC77276	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77276_Upin_Bot2011_COI	S16-06	1	BC77276_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77276	Sample	BC77276	targeted	Absent	Sample	Upin_Bot2011_COI	S16-06	COI	45	BC77276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-06	CTTTAATTACAGCGTTTTTATTGTTGT	138.516
BC77277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77277	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77277_Cjap_Sim2018_COI	S16-07	1	BC77277_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77277	Sample	BC77277	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-07	COI	45	BC77277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-07	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51754
BC77277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77277	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.002	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77277_Mgig_Bot2012_COI	S16-07	1	BC77277_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77277	Sample	BC77277	targeted	Present	Sample	Mgig_Bot2012_COI	S16-07	COI	45	BC77277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-07	TCTTATTCGTTGGAGACTTTATAACCCT	138.51754
BC77278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77278	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77278_Cmae_Bot2011_COI	S16-08	1	BC77278_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77278	Sample	BC77278	targeted	Absent	Sample	Cmae_Bot2011_COI	S16-08	COI	45	BC77278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-08	ATGAACAGTCTATCCTCCTTTAG	138.51838
BC77280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83901	WGS84	BC77280	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S16	3/12/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77280_Cjap_Sim2018_COI	S16-01	1	BC77280_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77280	Sample	BC77280	targeted	Absent	Sample	Cjap_Sim2018_COI	S16-01	COI	45	BC77280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-01	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50604
BC77280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83901	WGS84	BC77280	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.94	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S16	3/12/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77280_Mgig_Bot2012_COI	S16-01	1	BC77280_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77280	Sample	BC77280	targeted	Present	Sample	Mgig_Bot2012_COI	S16-01	COI	45	BC77280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S16-01	TCTTATTCGTTGGAGACTTTATAACCCT	138.50604
BC77281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77315	WGS84	BC77281	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77281_Cmae_Bot2011_COI	S17-01	1	BC77281_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77281	Sample	BC77281	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-01	COI	45	BC77281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-01	ATGAACAGTCTATCCTCCTTTAG	138.48546
BC77283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77394	WGS84	BC77283	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77283_Aamu_Bax2006_COI	S17-03	1	BC77283_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77283	Sample	BC77283	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-03	COI	45	BC77283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-03	GCACAACCGGGATCTTTACTTC	138.48586
BC77283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77394	WGS84	BC77283	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77283_Pcan_Bot2011_IGS	S17-03	1	BC77283_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77283	Sample	BC77283	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-03	IGS	45	BC77283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-03	CGTAATCCTCAGTACTGGCTA	138.48586
BC77283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77394	WGS84	BC77283	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.821	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77283_Sspa_Oph2007_COI	S17-03	1	BC77283_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77283	Sample	BC77283	targeted	Present	Sample	Sspa_Oph2007_COI	S17-03	COI	45	BC77283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-03	GAAGGCCTTCCGATGATC	138.48586
BC77283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77394	WGS84	BC77283	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77283_Upin_Bot2011_COI	S17-03	1	BC77283_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77283	Sample	BC77283	targeted	Absent	Sample	Upin_Bot2011_COI	S17-03	COI	45	BC77283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.48586
BC77284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77691	WGS84	BC77284	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77284_Cjap_Sim2018_COI	S17-04	1	BC77284_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77284	Sample	BC77284	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-04	COI	45	BC77284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51832
BC77284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77691	WGS84	BC77284	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.725	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77284_Mgig_Bot2012_COI	S17-04	1	BC77284_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77284	Sample	BC77284	targeted	Present	Sample	Mgig_Bot2012_COI	S17-04	COI	45	BC77284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.51832
BC77285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77779	WGS84	BC77285	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77285_Cmae_Bot2011_COI	S17-05	1	BC77285_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77285	Sample	BC77285	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-05	COI	45	BC77285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-05	ATGAACAGTCTATCCTCCTTTAG	138.51794
BC77287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80962	WGS84	BC77287	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77287_Aamu_Bax2006_COI	S17-07	1	BC77287_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77287	Sample	BC77287	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-07	COI	45	BC77287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-07	GCACAACCGGGATCTTTACTTC	138.51817
BC77287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80962	WGS84	BC77287	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77287_Pcan_Bot2011_IGS	S17-07	1	BC77287_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77287	Sample	BC77287	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-07	IGS	45	BC77287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-07	CGTAATCCTCAGTACTGGCTA	138.51817
BC77287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80962	WGS84	BC77287	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	43.404	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77287_Sspa_Oph2007_COI	S17-07	1	BC77287_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77287	Sample	BC77287	targeted	Present	Sample	Sspa_Oph2007_COI	S17-07	COI	45	BC77287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-07	GAAGGCCTTCCGATGATC	138.51817
BC77287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80962	WGS84	BC77287	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77287_Upin_Bot2011_COI	S17-07	1	BC77287_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77287	Sample	BC77287	targeted	Absent	Sample	Upin_Bot2011_COI	S17-07	COI	45	BC77287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.51817
BC77288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80998	WGS84	BC77288	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77288_Cjap_Sim2018_COI	S17-08	1	BC77288_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77288	Sample	BC77288	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-08	COI	45	BC77288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51955
BC77288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80998	WGS84	BC77288	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.93	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77288_Mgig_Bot2012_COI	S17-08	1	BC77288_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77288	Sample	BC77288	targeted	Present	Sample	Mgig_Bot2012_COI	S17-08	COI	45	BC77288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51955
BC77289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81059	WGS84	BC77289	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77289_Cmae_Bot2011_COI	S17-09	1	BC77289_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77289	Sample	BC77289	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-09	COI	45	BC77289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-09	ATGAACAGTCTATCCTCCTTTAG	138.52046
BC77290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84498	WGS84	BC77290	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77290_Aamu_Bax2006_COI	S17-10	1	BC77290_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77290	Sample	BC77290	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-10	COI	45	BC77290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-10	GCACAACCGGGATCTTTACTTC	138.49722
BC77290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84498	WGS84	BC77290	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77290_Pcan_Bot2011_IGS	S17-10	1	BC77290_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77290	Sample	BC77290	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-10	IGS	45	BC77290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-10	CGTAATCCTCAGTACTGGCTA	138.49722
BC77290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84498	WGS84	BC77290	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77290_Sspa_Oph2007_COI	S17-10	1	BC77290_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77290	Sample	BC77290	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-10	COI	45	BC77290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-10	GAAGGCCTTCCGATGATC	138.49722
BC77290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84498	WGS84	BC77290	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77290_Upin_Bot2011_COI	S17-10	1	BC77290_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77290	Sample	BC77290	targeted	Absent	Sample	Upin_Bot2011_COI	S17-10	COI	45	BC77290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-10	CTTTAATTACAGCGTTTTTATTGTTGT	138.49722
BC77291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.844	WGS84	BC77291	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77291_Cjap_Sim2018_COI	S17-11	1	BC77291_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77291	Sample	BC77291	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-11	COI	45	BC77291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-11	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.4977
BC77291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.844	WGS84	BC77291	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	42.52	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77291_Mgig_Bot2012_COI	S17-11	1	BC77291_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77291	Sample	BC77291	targeted	Present	Sample	Mgig_Bot2012_COI	S17-11	COI	45	BC77291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-11	TCTTATTCGTTGGAGACTTTATAACCCT	138.4977
BC77292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84305	WGS84	BC77292	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77292_Cmae_Bot2011_COI	S17-12	1	BC77292_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77292	Sample	BC77292	targeted	Absent	Sample	Cmae_Bot2011_COI	S17-12	COI	45	BC77292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-12	ATGAACAGTCTATCCTCCTTTAG	138.49884
BC77294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83721	WGS84	BC77294	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77294_Aamu_Bax2006_COI	S17-14	1	BC77294_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77294	Sample	BC77294	targeted	Absent	Sample	Aamu_Bax2006_COI	S17-14	COI	45	BC77294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-14	GCACAACCGGGATCTTTACTTC	138.51088
BC77294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83721	WGS84	BC77294	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77294_Pcan_Bot2011_IGS	S17-14	1	BC77294_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77294	Sample	BC77294	targeted	Absent	Sample	Pcan_Bot2011_IGS	S17-14	IGS	45	BC77294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-14	CGTAATCCTCAGTACTGGCTA	138.51088
BC77294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83721	WGS84	BC77294	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77294_Sspa_Oph2007_COI	S17-14	1	BC77294_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77294	Sample	BC77294	targeted	Absent	Sample	Sspa_Oph2007_COI	S17-14	COI	45	BC77294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-14	GAAGGCCTTCCGATGATC	138.51088
BC77294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83721	WGS84	BC77294	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77294_Upin_Bot2011_COI	S17-14	1	BC77294_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77294	Sample	BC77294	targeted	Absent	Sample	Upin_Bot2011_COI	S17-14	COI	45	BC77294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-14	CTTTAATTACAGCGTTTTTATTGTTGT	138.51088
BC77295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83581	WGS84	BC77295	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S17	26/03/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77295_Cjap_Sim2018_COI	S17-15	1	BC77295_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77295	Sample	BC77295	targeted	Absent	Sample	Cjap_Sim2018_COI	S17-15	COI	45	BC77295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50807
BC77295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83581	WGS84	BC77295	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.991	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S17	26/03/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77295_Mgig_Bot2012_COI	S17-15	1	BC77295_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77295	Sample	BC77295	targeted	Present	Sample	Mgig_Bot2012_COI	S17-15	COI	45	BC77295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S17-15	TCTTATTCGTTGGAGACTTTATAACCCT	138.50807
BC77296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83556	WGS84	BC77296	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77296_Cmae_Bot2011_COI	S18-01	1	BC77296_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77296	Sample	BC77296	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-01	COI	45	BC77296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-01	ATGAACAGTCTATCCTCCTTTAG	138.50784
BC77298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83661	WGS84	BC77298	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77298_Aamu_Bax2006_COI	S18-03	1	BC77298_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77298	Sample	BC77298	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-03	COI	45	BC77298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-03	GCACAACCGGGATCTTTACTTC	138.5099
BC77298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83661	WGS84	BC77298	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77298_Pcan_Bot2011_IGS	S18-03	1	BC77298_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77298	Sample	BC77298	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-03	IGS	45	BC77298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-03	CGTAATCCTCAGTACTGGCTA	138.5099
BC77298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83661	WGS84	BC77298	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.833	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77298_Sspa_Oph2007_COI	S18-03	1	BC77298_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77298	Sample	BC77298	targeted	Present	Sample	Sspa_Oph2007_COI	S18-03	COI	45	BC77298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-03	GAAGGCCTTCCGATGATC	138.5099
BC77298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83661	WGS84	BC77298	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77298_Upin_Bot2011_COI	S18-03	1	BC77298_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77298	Sample	BC77298	targeted	Absent	Sample	Upin_Bot2011_COI	S18-03	COI	45	BC77298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-03	CTTTAATTACAGCGTTTTTATTGTTGT	138.5099
BC77299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83696	WGS84	BC77299	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77299_Cjap_Sim2018_COI	S18-04	1	BC77299_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77299	Sample	BC77299	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-04	COI	45	BC77299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51075
BC77299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83696	WGS84	BC77299	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77299_Mgig_Bot2012_COI	S18-04	1	BC77299_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77299	Sample	BC77299	targeted	Absent	Sample	Mgig_Bot2012_COI	S18-04	COI	45	BC77299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-04	TCTTATTCGTTGGAGACTTTATAACCCT	138.51075
BC77300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83734	WGS84	BC77300	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77300_Cmae_Bot2011_COI	S18-05	1	BC77300_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77300	Sample	BC77300	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-05	COI	45	BC77300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-05	ATGAACAGTCTATCCTCCTTTAG	138.51042
BC77302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77302	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77302_Aamu_Bax2006_COI	S18-07	1	BC77302_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77302	Sample	BC77302	targeted	Absent	Sample	Aamu_Bax2006_COI	S18-07	COI	45	BC77302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-07	GCACAACCGGGATCTTTACTTC	138.51771
BC77302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77302	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77302_Pcan_Bot2011_IGS	S18-07	1	BC77302_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77302	Sample	BC77302	targeted	Absent	Sample	Pcan_Bot2011_IGS	S18-07	IGS	45	BC77302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-07	CGTAATCCTCAGTACTGGCTA	138.51771
BC77302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77302	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.848	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77302_Sspa_Oph2007_COI	S18-07	1	BC77302_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77302	Sample	BC77302	targeted	Present	Sample	Sspa_Oph2007_COI	S18-07	COI	45	BC77302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-07	GAAGGCCTTCCGATGATC	138.51771
BC77302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80911	WGS84	BC77302	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77302_Upin_Bot2011_COI	S18-07	1	BC77302_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77302	Sample	BC77302	targeted	Absent	Sample	Upin_Bot2011_COI	S18-07	COI	45	BC77302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-07	CTTTAATTACAGCGTTTTTATTGTTGT	138.51771
BC77303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77303	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77303_Cjap_Sim2018_COI	S18-08	1	BC77303_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77303	Sample	BC77303	targeted	Absent	Sample	Cjap_Sim2018_COI	S18-08	COI	45	BC77303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51854
BC77303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.80968	WGS84	BC77303	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.982	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S18	4/09/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77303_Mgig_Bot2012_COI	S18-08	1	BC77303_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77303	Sample	BC77303	targeted	Present	Sample	Mgig_Bot2012_COI	S18-08	COI	45	BC77303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-08	TCTTATTCGTTGGAGACTTTATAACCCT	138.51854
BC77304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81006	WGS84	BC77304	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S18	4/09/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77304_Cmae_Bot2011_COI	S18-09	1	BC77304_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77304	Sample	BC77304	targeted	Absent	Sample	Cmae_Bot2011_COI	S18-09	COI	45	BC77304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S18-09	ATGAACAGTCTATCCTCCTTTAG	138.51952
BC77306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77317	WGS84	BC77306	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77306_Aamu_Bax2006_COI	S19-01	1	BC77306_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77306	Sample	BC77306	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-01	COI	45	BC77306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-01	GCACAACCGGGATCTTTACTTC	138.48557
BC77306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77317	WGS84	BC77306	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77306_Pcan_Bot2011_IGS	S19-01	1	BC77306_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77306	Sample	BC77306	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-01	IGS	45	BC77306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-01	CGTAATCCTCAGTACTGGCTA	138.48557
BC77306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77317	WGS84	BC77306	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.029	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77306_Sspa_Oph2007_COI	S19-01	1	BC77306_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77306	Sample	BC77306	targeted	Present	Sample	Sspa_Oph2007_COI	S19-01	COI	45	BC77306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-01	GAAGGCCTTCCGATGATC	138.48557
BC77306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77317	WGS84	BC77306	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77306_Upin_Bot2011_COI	S19-01	1	BC77306_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77306	Sample	BC77306	targeted	Absent	Sample	Upin_Bot2011_COI	S19-01	COI	45	BC77306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-01	CTTTAATTACAGCGTTTTTATTGTTGT	138.48557
BC77307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77357	WGS84	BC77307	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77307_Cjap_Sim2018_COI	S19-02	1	BC77307_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77307	Sample	BC77307	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-02	COI	45	BC77307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-02	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48591
BC77307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77357	WGS84	BC77307	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77307_Mgig_Bot2012_COI	S19-02	1	BC77307_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77307	Sample	BC77307	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-02	COI	45	BC77307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-02	TCTTATTCGTTGGAGACTTTATAACCCT	138.48591
BC77308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.77295	WGS84	BC77308	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77308_Cmae_Bot2011_COI	S19-03	1	BC77308_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77308	Sample	BC77308	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-03	COI	45	BC77308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-03	ATGAACAGTCTATCCTCCTTTAG	138.48449
BC77310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7798	WGS84	BC77310	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77310_Cjap_Sim2018_COI	S19-05	1	BC77310_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77310	Sample	BC77310	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-05	COI	45	BC77310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-05	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51801
BC77310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.7798	WGS84	BC77310	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77310_Mgig_Bot2012_COI	S19-05	1	BC77310_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77310	Sample	BC77310	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-05	COI	45	BC77310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-05	TCTTATTCGTTGGAGACTTTATAACCCT	138.51801
BC77311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.78118	WGS84	BC77311	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.443	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77311_Cmae_Bot2011_COI	S19-06	1	BC77311_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77311	Sample	BC77311	targeted	Present	Sample	Cmae_Bot2011_COI	S19-06	COI	45	BC77311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-06	ATGAACAGTCTATCCTCCTTTAG	138.5172
BC77313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84552	WGS84	BC77313	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77313_Aamu_Bax2006_COI	S19-08	1	BC77313_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77313	Sample	BC77313	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-08	COI	45	BC77313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-08	GCACAACCGGGATCTTTACTTC	138.49703
BC77313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84552	WGS84	BC77313	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77313_Pcan_Bot2011_IGS	S19-08	1	BC77313_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77313	Sample	BC77313	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-08	IGS	45	BC77313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-08	CGTAATCCTCAGTACTGGCTA	138.49703
BC77313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84552	WGS84	BC77313	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77313_Sspa_Oph2007_COI	S19-08	1	BC77313_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77313	Sample	BC77313	targeted	Absent	Sample	Sspa_Oph2007_COI	S19-08	COI	45	BC77313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-08	GAAGGCCTTCCGATGATC	138.49703
BC77313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84552	WGS84	BC77313	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77313_Upin_Bot2011_COI	S19-08	1	BC77313_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77313	Sample	BC77313	targeted	Absent	Sample	Upin_Bot2011_COI	S19-08	COI	45	BC77313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-08	CTTTAATTACAGCGTTTTTATTGTTGT	138.49703
BC77314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84334	WGS84	BC77314	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77314_Cjap_Sim2018_COI	S19-09	1	BC77314_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77314	Sample	BC77314	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-09	COI	45	BC77314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-09	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49677
BC77314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.84334	WGS84	BC77314	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77314_Mgig_Bot2012_COI	S19-09	1	BC77314_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77314	Sample	BC77314	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-09	COI	45	BC77314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-09	TCTTATTCGTTGGAGACTTTATAACCCT	138.49677
BC77315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83658	WGS84	BC77315	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77315_Cmae_Bot2011_COI	S19-10	1	BC77315_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77315	Sample	BC77315	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-10	COI	45	BC77315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-10	ATGAACAGTCTATCCTCCTTTAG	138.50835
BC77317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83647	WGS84	BC77317	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77317_Aamu_Bax2006_COI	S19-12	1	BC77317_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77317	Sample	BC77317	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-12	COI	45	BC77317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-12	GCACAACCGGGATCTTTACTTC	138.50948
BC77317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83647	WGS84	BC77317	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77317_Pcan_Bot2011_IGS	S19-12	1	BC77317_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77317	Sample	BC77317	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-12	IGS	45	BC77317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-12	CGTAATCCTCAGTACTGGCTA	138.50948
BC77317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83647	WGS84	BC77317	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.99	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77317_Sspa_Oph2007_COI	S19-12	1	BC77317_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77317	Sample	BC77317	targeted	Present	Sample	Sspa_Oph2007_COI	S19-12	COI	45	BC77317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-12	GAAGGCCTTCCGATGATC	138.50948
BC77317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.83647	WGS84	BC77317	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77317_Upin_Bot2011_COI	S19-12	1	BC77317_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77317	Sample	BC77317	targeted	Absent	Sample	Upin_Bot2011_COI	S19-12	COI	45	BC77317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-12	CTTTAATTACAGCGTTTTTATTGTTGT	138.50948
BC77318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8092	WGS84	BC77318	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Theil MJ, Giblot-Ducray D, Deveney MR (2020) Molecular surveillance for Asian Paddle Crab in the Port River - Barker Inlet. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2020/000289-1. SARDI Research Report Series No. 1068., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BC77318_Cjap_Sim2018_COI	S19-13	1	BC77318_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77318	Sample	BC77318	targeted	Absent	Sample	Cjap_Sim2018_COI	S19-13	COI	45	BC77318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-13	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.5177
BC77318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.8092	WGS84	BC77318	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Deveney MR, Wiltshire KH, Lieu YN, Buss JJ, Moody NJG, Mohr PA, Ellard KA (2020) Advanced surveillance for OsHV-1 microvariant. In 'Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster diseases'. (Eds MR Deveney and KH Wiltshire) pp. 1 - 43. (Fisheries Research and Development Corporation Deakin, ACT)	Oyster_CRC-P_surveys-S19	29/04/2019	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BC77318_Mgig_Bot2012_COI	S19-13	1	BC77318_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77318	Sample	BC77318	targeted	Absent	Sample	Mgig_Bot2012_COI	S19-13	COI	45	BC77318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-13	TCTTATTCGTTGGAGACTTTATAACCCT	138.5177
BC77319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81009	WGS84	BC77319	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BC77319_Cmae_Bot2011_COI	S19-14	1	BC77319_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77319	Sample	BC77319	targeted	Absent	Sample	Cmae_Bot2011_COI	S19-14	COI	45	BC77319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-14	ATGAACAGTCTATCCTCCTTTAG	138.52
BC77320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81046	WGS84	BC77320	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BC77320_Aamu_Bax2006_COI	S19-15	1	BC77320_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77320	Sample	BC77320	targeted	Absent	Sample	Aamu_Bax2006_COI	S19-15	COI	45	BC77320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-15	GCACAACCGGGATCTTTACTTC	138.52139
BC77320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81046	WGS84	BC77320	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BC77320_Pcan_Bot2011_IGS	S19-15	1	BC77320_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77320	Sample	BC77320	targeted	Absent	Sample	Pcan_Bot2011_IGS	S19-15	IGS	45	BC77320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-15	CGTAATCCTCAGTACTGGCTA	138.52139
BC77320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81046	WGS84	BC77320	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.897	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BC77320_Sspa_Oph2007_COI	S19-15	1	BC77320_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77320	Sample	BC77320	targeted	Present	Sample	Sspa_Oph2007_COI	S19-15	COI	45	BC77320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-15	GAAGGCCTTCCGATGATC	138.52139
BC77320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_13009	-34.81046	WGS84	BC77320	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH (2021) Designing molecular surveillance for marine pests to support domestic ballast water management. Report to Department of Agriculture, Water and the Environment. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2021/000152-1. SARDI Research Report Series No. 1095., Adelaide.	Oyster_CRC-P_surveys-S19	29/04/2019	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BC77320_Upin_Bot2011_COI	S19-15	1	BC77320_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BC77320	Sample	BC77320	targeted	Absent	Sample	Upin_Bot2011_COI	S19-15	COI	45	BC77320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	S19-15	CTTTAATTACAGCGTTTTTATTGTTGT	138.52139
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28891_Aamu_Bax2006_COI	POR-B30	1	BD28891_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	GCACAACCGGGATCTTTACTTC	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28891_Pcan_Bot2011_IGS	POR-B30	1	BD28891_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B30	IGS	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	CGTAATCCTCAGTACTGGCTA	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28891_Pper_Dia2013_COI	POR-B30	1	BD28891_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	CTTAGTGGCATTAATTCGDAATCC	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28891_Sspa_Oph2007_COI	POR-B30	1	BD28891_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	GAAGGCCTTCCGATGATC	141.62277
BD28891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34516	WGS84	BD28891	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.115	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28891_Upin_Bot2011_COI	POR-B30	1	BD28891_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28891	Sample	BD28891	targeted	Present	Sample	Upin_Bot2011_COI	POR-B30	COI	45	BD28891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B30	CTTTAATTACAGCGTTTTTATTGTTGT	141.62277
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28892_Cjap_Sim2018_COI	POR-B31	1	BD28892_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28892_Esin_And2018_Cyb	POR-B31	1	BD28892_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B31	Cytb	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	ACCCCTCCTCATATCCAACCA	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28892_Mgig_Bot2012_COI	POR-B31	1	BD28892_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	TCTTATTCGTTGGAGACTTTATAACCCT	141.61982
BD28892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3498	WGS84	BD28892	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28892_Mjap_Gib2022_COI	POR-B31	1	BD28892_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28892	Sample	BD28892	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B31	COI	45	BD28892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B31	AATTTAATTGTGACTAGGCATGGTCTG	141.61982
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28893_Cmae_Bot2011_COI	POR-B32	1	BD28893_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	ATGAACAGTCTATCCTCCTTTAG	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28893_Hsan_Wil2021_COI	POR-B32	1	BD28893_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	CCTGGGCCGGTATAGTAGGT	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28893_Mros_Gib2022_COI	POR-B32	1	BD28893_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28893_Msal_Bot2012_COI	POR-B32	1	BD28893_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	GYTAGTTCCRATGATGTTAGCTG	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28893_Mstr_Wil2021_COI	POR-B32	1	BD28893_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	GTAGTGGTAACTACTCATGCTTTA	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28893_Pvir_Dia2013_COI	POR-B32	1	BD28893_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	CTTAGTGGCATTAATTCGDAATCC	141.62082
BD28893	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34787	WGS84	BD28893	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28893_Rhar_Sim2018_COI	POR-B32	1	BD28893_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28893	Sample	BD28893	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B32	COI	45	BD28893	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B32	CTGCTGCTATTGCTCATG	141.62082
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28895_Aamu_Bax2006_COI	POR-B34	1	BD28895_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	GCACAACCGGGATCTTTACTTC	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28895_Pcan_Bot2011_IGS	POR-B34	1	BD28895_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B34	IGS	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	CGTAATCCTCAGTACTGGCTA	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28895_Pper_Dia2013_COI	POR-B34	1	BD28895_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	CTTAGTGGCATTAATTCGDAATCC	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28895_Sspa_Oph2007_COI	POR-B34	1	BD28895_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	GAAGGCCTTCCGATGATC	141.61843
BD28895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34851	WGS84	BD28895	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.487	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28895_Upin_Bot2011_COI	POR-B34	1	BD28895_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28895	Sample	BD28895	targeted	Present	Sample	Upin_Bot2011_COI	POR-B34	COI	45	BD28895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B34	CTTTAATTACAGCGTTTTTATTGTTGT	141.61843
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28896_Cjap_Sim2018_COI	POR-B22	1	BD28896_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28896_Esin_And2018_Cyb	POR-B22	1	BD28896_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B22	Cytb	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	ACCCCTCCTCATATCCAACCA	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28896_Mgig_Bot2012_COI	POR-B22	1	BD28896_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	TCTTATTCGTTGGAGACTTTATAACCCT	141.62235
BD28896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34604	WGS84	BD28896	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28896_Mjap_Gib2022_COI	POR-B22	1	BD28896_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28896	Sample	BD28896	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B22	COI	45	BD28896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B22	AATTTAATTGTGACTAGGCATGGTCTG	141.62235
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28897_Cmae_Bot2011_COI	POR-B21	1	BD28897_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	ATGAACAGTCTATCCTCCTTTAG	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28897_Hsan_Wil2021_COI	POR-B21	1	BD28897_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	CCTGGGCCGGTATAGTAGGT	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28897_Mros_Gib2022_COI	POR-B21	1	BD28897_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28897_Msal_Bot2012_COI	POR-B21	1	BD28897_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	GYTAGTTCCRATGATGTTAGCTG	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28897_Mstr_Wil2021_COI	POR-B21	1	BD28897_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	GTAGTGGTAACTACTCATGCTTTA	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28897_Pvir_Dia2013_COI	POR-B21	1	BD28897_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	CTTAGTGGCATTAATTCGDAATCC	141.62176
BD28897	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34549	WGS84	BD28897	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28897_Rhar_Sim2018_COI	POR-B21	1	BD28897_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28897	Sample	BD28897	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B21	COI	45	BD28897	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B21	CTGCTGCTATTGCTCATG	141.62176
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28899_Aamu_Bax2006_COI	POR-B19	1	BD28899_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	GCACAACCGGGATCTTTACTTC	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28899_Pcan_Bot2011_IGS	POR-B19	1	BD28899_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B19	IGS	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	CGTAATCCTCAGTACTGGCTA	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28899_Pper_Dia2013_COI	POR-B19	1	BD28899_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	CTTAGTGGCATTAATTCGDAATCC	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28899_Sspa_Oph2007_COI	POR-B19	1	BD28899_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	GAAGGCCTTCCGATGATC	141.6189
BD28899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34564	WGS84	BD28899	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28899_Upin_Bot2011_COI	POR-B19	1	BD28899_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28899	Sample	BD28899	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B19	COI	45	BD28899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B19	CTTTAATTACAGCGTTTTTATTGTTGT	141.6189
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28900_Cjap_Sim2018_COI	POR-B18	1	BD28900_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28900_Esin_And2018_Cyb	POR-B18	1	BD28900_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B18	Cytb	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	ACCCCTCCTCATATCCAACCA	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28900_Mgig_Bot2012_COI	POR-B18	1	BD28900_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	TCTTATTCGTTGGAGACTTTATAACCCT	141.61759
BD28900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34568	WGS84	BD28900	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28900_Mjap_Gib2022_COI	POR-B18	1	BD28900_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28900	Sample	BD28900	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B18	COI	45	BD28900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B18	AATTTAATTGTGACTAGGCATGGTCTG	141.61759
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28901_Cmae_Bot2011_COI	POR-B17	1	BD28901_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	ATGAACAGTCTATCCTCCTTTAG	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28901_Hsan_Wil2021_COI	POR-B17	1	BD28901_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	CCTGGGCCGGTATAGTAGGT	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28901_Mros_Gib2022_COI	POR-B17	1	BD28901_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28901_Msal_Bot2012_COI	POR-B17	1	BD28901_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	GYTAGTTCCRATGATGTTAGCTG	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28901_Mstr_Wil2021_COI	POR-B17	1	BD28901_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	GTAGTGGTAACTACTCATGCTTTA	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28901_Pvir_Dia2013_COI	POR-B17	1	BD28901_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	CTTAGTGGCATTAATTCGDAATCC	141.61616
BD28901	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34573	WGS84	BD28901	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28901_Rhar_Sim2018_COI	POR-B17	1	BD28901_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28901	Sample	BD28901	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B17	COI	45	BD28901	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B17	CTGCTGCTATTGCTCATG	141.61616
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28903_Aamu_Bax2006_COI	POR-B15	1	BD28903_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	GCACAACCGGGATCTTTACTTC	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28903_Pcan_Bot2011_IGS	POR-B15	1	BD28903_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B15	IGS	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	CGTAATCCTCAGTACTGGCTA	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28903_Pper_Dia2013_COI	POR-B15	1	BD28903_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	CTTAGTGGCATTAATTCGDAATCC	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28903_Sspa_Oph2007_COI	POR-B15	1	BD28903_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	GAAGGCCTTCCGATGATC	141.61341
BD28903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3459	WGS84	BD28903	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.058	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28903_Upin_Bot2011_COI	POR-B15	1	BD28903_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28903	Sample	BD28903	targeted	Present	Sample	Upin_Bot2011_COI	POR-B15	COI	45	BD28903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B15	CTTTAATTACAGCGTTTTTATTGTTGT	141.61341
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28904_Cjap_Sim2018_COI	POR-B14	1	BD28904_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28904_Esin_And2018_Cyb	POR-B14	1	BD28904_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B14	Cytb	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	ACCCCTCCTCATATCCAACCA	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28904_Mgig_Bot2012_COI	POR-B14	1	BD28904_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	TCTTATTCGTTGGAGACTTTATAACCCT	141.61401
BD28904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34494	WGS84	BD28904	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28904_Mjap_Gib2022_COI	POR-B14	1	BD28904_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28904	Sample	BD28904	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B14	COI	45	BD28904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B14	AATTTAATTGTGACTAGGCATGGTCTG	141.61401
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28905_Cmae_Bot2011_COI	POR-B13	1	BD28905_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	ATGAACAGTCTATCCTCCTTTAG	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28905_Hsan_Wil2021_COI	POR-B13	1	BD28905_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	CCTGGGCCGGTATAGTAGGT	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.321	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28905_Mros_Gib2022_COI	POR-B13	1	BD28905_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Present	Sample	Mros_Gib2022_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28905_Msal_Bot2012_COI	POR-B13	1	BD28905_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	GYTAGTTCCRATGATGTTAGCTG	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28905_Mstr_Wil2021_COI	POR-B13	1	BD28905_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	GTAGTGGTAACTACTCATGCTTTA	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28905_Pvir_Dia2013_COI	POR-B13	1	BD28905_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	CTTAGTGGCATTAATTCGDAATCC	141.61265
BD28905	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34495	WGS84	BD28905	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28905_Rhar_Sim2018_COI	POR-B13	1	BD28905_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28905	Sample	BD28905	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B13	COI	45	BD28905	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B13	CTGCTGCTATTGCTCATG	141.61265
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28907_Aamu_Bax2006_COI	POR-B11	1	BD28907_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	GCACAACCGGGATCTTTACTTC	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28907_Pcan_Bot2011_IGS	POR-B11	1	BD28907_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B11	IGS	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	CGTAATCCTCAGTACTGGCTA	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28907_Pper_Dia2013_COI	POR-B11	1	BD28907_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	CTTAGTGGCATTAATTCGDAATCC	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28907_Sspa_Oph2007_COI	POR-B11	1	BD28907_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	GAAGGCCTTCCGATGATC	141.62078
BD28907	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34885	WGS84	BD28907	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28907_Upin_Bot2011_COI	POR-B11	1	BD28907_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28907	Sample	BD28907	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B11	COI	45	BD28907	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B11	CTTTAATTACAGCGTTTTTATTGTTGT	141.62078
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28909_Cmae_Bot2011_COI	POR-B10	1	BD28909_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	ATGAACAGTCTATCCTCCTTTAG	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28909_Hsan_Wil2021_COI	POR-B10	1	BD28909_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	CCTGGGCCGGTATAGTAGGT	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28909_Mros_Gib2022_COI	POR-B10	1	BD28909_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28909_Msal_Bot2012_COI	POR-B10	1	BD28909_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	GYTAGTTCCRATGATGTTAGCTG	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28909_Mstr_Wil2021_COI	POR-B10	1	BD28909_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	GTAGTGGTAACTACTCATGCTTTA	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28909_Pvir_Dia2013_COI	POR-B10	1	BD28909_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	CTTAGTGGCATTAATTCGDAATCC	141.62026
BD28909	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34731	WGS84	BD28909	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28909_Rhar_Sim2018_COI	POR-B10	1	BD28909_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28909	Sample	BD28909	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B10	COI	45	BD28909	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B10	CTGCTGCTATTGCTCATG	141.62026
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28910_Aamu_Bax2006_COI	POR-B09	1	BD28910_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	GCACAACCGGGATCTTTACTTC	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28910_Pcan_Bot2011_IGS	POR-B09	1	BD28910_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B09	IGS	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	CGTAATCCTCAGTACTGGCTA	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28910_Pper_Dia2013_COI	POR-B09	1	BD28910_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	CTTAGTGGCATTAATTCGDAATCC	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28910_Sspa_Oph2007_COI	POR-B09	1	BD28910_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	GAAGGCCTTCCGATGATC	141.61892
BD28910	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34738	WGS84	BD28910	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28910_Upin_Bot2011_COI	POR-B09	1	BD28910_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28910	Sample	BD28910	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B09	COI	45	BD28910	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B09	CTTTAATTACAGCGTTTTTATTGTTGT	141.61892
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28911_Cjap_Sim2018_COI	POR-B08	1	BD28911_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28911_Esin_And2018_Cyb	POR-B08	1	BD28911_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B08	Cytb	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	ACCCCTCCTCATATCCAACCA	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28911_Mgig_Bot2012_COI	POR-B08	1	BD28911_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	TCTTATTCGTTGGAGACTTTATAACCCT	141.61753
BD28911	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34745	WGS84	BD28911	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28911_Mjap_Gib2022_COI	POR-B08	1	BD28911_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28911	Sample	BD28911	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B08	COI	45	BD28911	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B08	AATTTAATTGTGACTAGGCATGGTCTG	141.61753
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28912_Cmae_Bot2011_COI	POR-B07	1	BD28912_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	ATGAACAGTCTATCCTCCTTTAG	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28912_Hsan_Wil2021_COI	POR-B07	1	BD28912_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	CCTGGGCCGGTATAGTAGGT	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.833	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28912_Mros_Gib2022_COI	POR-B07	1	BD28912_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Present	Sample	Mros_Gib2022_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28912_Msal_Bot2012_COI	POR-B07	1	BD28912_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	GYTAGTTCCRATGATGTTAGCTG	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28912_Mstr_Wil2021_COI	POR-B07	1	BD28912_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	GTAGTGGTAACTACTCATGCTTTA	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28912_Pvir_Dia2013_COI	POR-B07	1	BD28912_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	CTTAGTGGCATTAATTCGDAATCC	141.61615
BD28912	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34755	WGS84	BD28912	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28912_Rhar_Sim2018_COI	POR-B07	1	BD28912_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28912	Sample	BD28912	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B07	COI	45	BD28912	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B07	CTGCTGCTATTGCTCATG	141.61615
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28914_Aamu_Bax2006_COI	POR-B05	1	BD28914_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	GCACAACCGGGATCTTTACTTC	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28914_Pcan_Bot2011_IGS	POR-B05	1	BD28914_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B05	IGS	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	CGTAATCCTCAGTACTGGCTA	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28914_Pper_Dia2013_COI	POR-B05	1	BD28914_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	CTTAGTGGCATTAATTCGDAATCC	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28914_Sspa_Oph2007_COI	POR-B05	1	BD28914_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	GAAGGCCTTCCGATGATC	141.61197
BD28914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34776	WGS84	BD28914	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	34.911	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28914_Upin_Bot2011_COI	POR-B05	1	BD28914_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28914	Sample	BD28914	targeted	Present	Sample	Upin_Bot2011_COI	POR-B05	COI	45	BD28914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B05	CTTTAATTACAGCGTTTTTATTGTTGT	141.61197
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28915_Cjap_Sim2018_COI	POR-B04	1	BD28915_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28915_Esin_And2018_Cyb	POR-B04	1	BD28915_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B04	Cytb	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	ACCCCTCCTCATATCCAACCA	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28915_Mgig_Bot2012_COI	POR-B04	1	BD28915_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	TCTTATTCGTTGGAGACTTTATAACCCT	141.61159
BD28915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34784	WGS84	BD28915	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28915_Mjap_Gib2022_COI	POR-B04	1	BD28915_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28915	Sample	BD28915	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B04	COI	45	BD28915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B04	AATTTAATTGTGACTAGGCATGGTCTG	141.61159
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28916_Cmae_Bot2011_COI	POR-B03	1	BD28916_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	ATGAACAGTCTATCCTCCTTTAG	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28916_Hsan_Wil2021_COI	POR-B03	1	BD28916_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	CCTGGGCCGGTATAGTAGGT	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28916_Mros_Gib2022_COI	POR-B03	1	BD28916_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28916_Msal_Bot2012_COI	POR-B03	1	BD28916_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	GYTAGTTCCRATGATGTTAGCTG	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28916_Mstr_Wil2021_COI	POR-B03	1	BD28916_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	GTAGTGGTAACTACTCATGCTTTA	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28916_Pvir_Dia2013_COI	POR-B03	1	BD28916_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	CTTAGTGGCATTAATTCGDAATCC	141.61129
BD28916	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34852	WGS84	BD28916	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28916_Rhar_Sim2018_COI	POR-B03	1	BD28916_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28916	Sample	BD28916	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B03	COI	45	BD28916	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B03	CTGCTGCTATTGCTCATG	141.61129
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28918_Aamu_Bax2006_COI	POR-B01	1	BD28918_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	GCACAACCGGGATCTTTACTTC	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28918_Pcan_Bot2011_IGS	POR-B01	1	BD28918_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B01	IGS	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	CGTAATCCTCAGTACTGGCTA	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28918_Pper_Dia2013_COI	POR-B01	1	BD28918_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	CTTAGTGGCATTAATTCGDAATCC	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28918_Sspa_Oph2007_COI	POR-B01	1	BD28918_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	GAAGGCCTTCCGATGATC	141.60984
BD28918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34938	WGS84	BD28918	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28918_Upin_Bot2011_COI	POR-B01	1	BD28918_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28918	Sample	BD28918	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B01	COI	45	BD28918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B01	CTTTAATTACAGCGTTTTTATTGTTGT	141.60984
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81115	WGS84	BD28919	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28919_Asen_Wil2025_COI	ADL-B35	1	BD28919_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	GTTGTTGTTACAACACATGCTCTTTTG	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28919_Cjap_Sim2018_COI	ADL-B35	1	BD28919_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28919_Esin_And2018_Cyb	ADL-B35	1	BD28919_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B35	Cytb	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	ACCCCTCCTCATATCCAACCA	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.726	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28919_Mgig_Bot2012_COI	ADL-B35	1	BD28919_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	TCTTATTCGTTGGAGACTTTATAACCCT	138.51463
BD28919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81115	WGS84	BD28919	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28919_Mjap_Gib2022_COI	ADL-B35	1	BD28919_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28919	Sample	BD28919	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B35	COI	45	BD28919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B35	AATTTAATTGTGACTAGGCATGGTCTG	138.51463
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28921_Aamu_Bax2006_COI	ADL-B33	1	BD28921_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	GCACAACCGGGATCTTTACTTC	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28921_Pcan_Bot2011_IGS	ADL-B33	1	BD28921_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B33	IGS	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	CGTAATCCTCAGTACTGGCTA	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28921_Pper_Dia2013_COI	ADL-B33	1	BD28921_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	CTTAGTGGCATTAATTCGDAATCC	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28921_Sspa_Oph2007_COI	ADL-B33	1	BD28921_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	GAAGGCCTTCCGATGATC	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81419	WGS84	BD28921	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28921_Upin_Bot2011_COI	ADL-B33	1	BD28921_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	CTTTAATTACAGCGTTTTTATTGTTGT	138.51399
BD28921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81419	WGS84	BD28921	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28921_Vgib_Wil2025_COI	ADL-B33	1	BD28921_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28921	Sample	BD28921	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B33	COI	45	BD28921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B33	CCCCTCTTTACAAGTTCTTTAGGGT	138.51399
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.81518	WGS84	BD28922	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28922_Asen_Wil2025_COI	ADL-B32	1	BD28922_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	GTTGTTGTTACAACACATGCTCTTTTG	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28922_Cjap_Sim2018_COI	ADL-B32	1	BD28922_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28922_Esin_And2018_Cyb	ADL-B32	1	BD28922_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B32	Cytb	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	ACCCCTCCTCATATCCAACCA	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.517	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28922_Mgig_Bot2012_COI	ADL-B32	1	BD28922_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	TCTTATTCGTTGGAGACTTTATAACCCT	138.51323
BD28922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81518	WGS84	BD28922	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28922_Mjap_Gib2022_COI	ADL-B32	1	BD28922_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28922	Sample	BD28922	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B32	COI	45	BD28922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B32	AATTTAATTGTGACTAGGCATGGTCTG	138.51323
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28923_Cmae_Bot2011_COI	ADL-B31	1	BD28923_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	ATGAACAGTCTATCCTCCTTTAG	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28923_Hsan_Wil2021_COI	ADL-B31	1	BD28923_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	CCTGGGCCGGTATAGTAGGT	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28923_Mros_Gib2022_COI	ADL-B31	1	BD28923_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28923_Msal_Bot2012_COI	ADL-B31	1	BD28923_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	GYTAGTTCCRATGATGTTAGCTG	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28923_Mstr_Wil2021_COI	ADL-B31	1	BD28923_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	GTAGTGGTAACTACTCATGCTTTA	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28923_Pvir_Dia2013_COI	ADL-B31	1	BD28923_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	CTTAGTGGCATTAATTCGDAATCC	138.51405
BD28923	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81627	WGS84	BD28923	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28923_Rhar_Sim2018_COI	ADL-B31	1	BD28923_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28923	Sample	BD28923	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B31	COI	45	BD28923	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B31	CTGCTGCTATTGCTCATG	138.51405
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28925_Aamu_Bax2006_COI	ADL-B29	1	BD28925_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	GCACAACCGGGATCTTTACTTC	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28925_Pcan_Bot2011_IGS	ADL-B29	1	BD28925_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B29	IGS	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	CGTAATCCTCAGTACTGGCTA	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28925_Pper_Dia2013_COI	ADL-B29	1	BD28925_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	CTTAGTGGCATTAATTCGDAATCC	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28925_Sspa_Oph2007_COI	ADL-B29	1	BD28925_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	GAAGGCCTTCCGATGATC	138.51263
BD28925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81832	WGS84	BD28925	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28925_Upin_Bot2011_COI	ADL-B29	1	BD28925_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28925	Sample	BD28925	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B29	COI	45	BD28925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B29	CTTTAATTACAGCGTTTTTATTGTTGT	138.51263
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28926_Cjap_Sim2018_COI	ADL-B28	1	BD28926_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28926_Esin_And2018_Cyb	ADL-B28	1	BD28926_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B28	Cytb	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	ACCCCTCCTCATATCCAACCA	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.967	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28926_Mgig_Bot2012_COI	ADL-B28	1	BD28926_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	TCTTATTCGTTGGAGACTTTATAACCCT	138.51205
BD28926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.81933	WGS84	BD28926	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28926_Mjap_Gib2022_COI	ADL-B28	1	BD28926_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28926	Sample	BD28926	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B28	COI	45	BD28926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B28	AATTTAATTGTGACTAGGCATGGTCTG	138.51205
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28927_Cmae_Bot2011_COI	ADL-B27	1	BD28927_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	ATGAACAGTCTATCCTCCTTTAG	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28927_Hsan_Wil2021_COI	ADL-B27	1	BD28927_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	CCTGGGCCGGTATAGTAGGT	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28927_Mros_Gib2022_COI	ADL-B27	1	BD28927_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28927_Msal_Bot2012_COI	ADL-B27	1	BD28927_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	GYTAGTTCCRATGATGTTAGCTG	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28927_Mstr_Wil2021_COI	ADL-B27	1	BD28927_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	GTAGTGGTAACTACTCATGCTTTA	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28927_Pvir_Dia2013_COI	ADL-B27	1	BD28927_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	CTTAGTGGCATTAATTCGDAATCC	138.51146
BD28927	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82041	WGS84	BD28927	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28927_Rhar_Sim2018_COI	ADL-B27	1	BD28927_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28927	Sample	BD28927	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B27	COI	45	BD28927	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B27	CTGCTGCTATTGCTCATG	138.51146
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28929_Aamu_Bax2006_COI	ADL-B25	1	BD28929_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	GCACAACCGGGATCTTTACTTC	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28929_Pcan_Bot2011_IGS	ADL-B25	1	BD28929_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B25	IGS	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	CGTAATCCTCAGTACTGGCTA	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28929_Pper_Dia2013_COI	ADL-B25	1	BD28929_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	CTTAGTGGCATTAATTCGDAATCC	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28929_Sspa_Oph2007_COI	ADL-B25	1	BD28929_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	GAAGGCCTTCCGATGATC	138.51172
BD28929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82254	WGS84	BD28929	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28929_Upin_Bot2011_COI	ADL-B25	1	BD28929_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28929	Sample	BD28929	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B25	COI	45	BD28929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B25	CTTTAATTACAGCGTTTTTATTGTTGT	138.51172
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28930_Cmae_Bot2011_COI	ADL-B24	1	BD28930_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	ATGAACAGTCTATCCTCCTTTAG	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28930_Hsan_Wil2021_COI	ADL-B24	1	BD28930_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	CCTGGGCCGGTATAGTAGGT	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28930_Mros_Gib2022_COI	ADL-B24	1	BD28930_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28930_Msal_Bot2012_COI	ADL-B24	1	BD28930_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	GYTAGTTCCRATGATGTTAGCTG	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28930_Mstr_Wil2021_COI	ADL-B24	1	BD28930_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	GTAGTGGTAACTACTCATGCTTTA	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28930_Pvir_Dia2013_COI	ADL-B24	1	BD28930_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	CTTAGTGGCATTAATTCGDAATCC	138.51033
BD28930	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82248	WGS84	BD28930	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28930_Rhar_Sim2018_COI	ADL-B24	1	BD28930_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28930	Sample	BD28930	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B24	COI	45	BD28930	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B24	CTGCTGCTATTGCTCATG	138.51033
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28932_Aamu_Bax2006_COI	ADL-B22	1	BD28932_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	GCACAACCGGGATCTTTACTTC	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28932_Pcan_Bot2011_IGS	ADL-B22	1	BD28932_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B22	IGS	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	CGTAATCCTCAGTACTGGCTA	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28932_Pper_Dia2013_COI	ADL-B22	1	BD28932_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	CTTAGTGGCATTAATTCGDAATCC	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28932_Sspa_Oph2007_COI	ADL-B22	1	BD28932_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	GAAGGCCTTCCGATGATC	138.50953
BD28932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82356	WGS84	BD28932	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28932_Upin_Bot2011_COI	ADL-B22	1	BD28932_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28932	Sample	BD28932	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B22	COI	45	BD28932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B22	CTTTAATTACAGCGTTTTTATTGTTGT	138.50953
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28933_Cjap_Sim2018_COI	ADL-B21	1	BD28933_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28933_Esin_And2018_Cyb	ADL-B21	1	BD28933_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B21	Cytb	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	ACCCCTCCTCATATCCAACCA	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.217	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28933_Mgig_Bot2012_COI	ADL-B21	1	BD28933_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	TCTTATTCGTTGGAGACTTTATAACCCT	138.51072
BD28933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82424	WGS84	BD28933	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28933_Mjap_Gib2022_COI	ADL-B21	1	BD28933_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28933	Sample	BD28933	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B21	COI	45	BD28933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B21	AATTTAATTGTGACTAGGCATGGTCTG	138.51072
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28934_Cmae_Bot2011_COI	ADL-B20	1	BD28934_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	ATGAACAGTCTATCCTCCTTTAG	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28934_Hsan_Wil2021_COI	ADL-B20	1	BD28934_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	CCTGGGCCGGTATAGTAGGT	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28934_Mros_Gib2022_COI	ADL-B20	1	BD28934_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28934_Msal_Bot2012_COI	ADL-B20	1	BD28934_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	GYTAGTTCCRATGATGTTAGCTG	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28934_Mstr_Wil2021_COI	ADL-B20	1	BD28934_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	GTAGTGGTAACTACTCATGCTTTA	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28934_Pvir_Dia2013_COI	ADL-B20	1	BD28934_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	CTTAGTGGCATTAATTCGDAATCC	138.50884
BD28934	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82459	WGS84	BD28934	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28934_Rhar_Sim2018_COI	ADL-B20	1	BD28934_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28934	Sample	BD28934	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B20	COI	45	BD28934	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B20	CTGCTGCTATTGCTCATG	138.50884
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28936_Aamu_Bax2006_COI	ADL-B18	1	BD28936_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	GCACAACCGGGATCTTTACTTC	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28936_Pcan_Bot2011_IGS	ADL-B18	1	BD28936_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B18	IGS	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	CGTAATCCTCAGTACTGGCTA	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28936_Pper_Dia2013_COI	ADL-B18	1	BD28936_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	CTTAGTGGCATTAATTCGDAATCC	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28936_Sspa_Oph2007_COI	ADL-B18	1	BD28936_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	GAAGGCCTTCCGATGATC	138.50817
BD28936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82562	WGS84	BD28936	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28936_Upin_Bot2011_COI	ADL-B18	1	BD28936_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28936	Sample	BD28936	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B18	COI	45	BD28936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B18	CTTTAATTACAGCGTTTTTATTGTTGT	138.50817
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28937_Cjap_Sim2018_COI	ADL-B17	1	BD28937_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28937_Esin_And2018_Cyb	ADL-B17	1	BD28937_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B17	Cytb	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	ACCCCTCCTCATATCCAACCA	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	29.406	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28937_Mgig_Bot2012_COI	ADL-B17	1	BD28937_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	TCTTATTCGTTGGAGACTTTATAACCCT	138.50901
BD28937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82668	WGS84	BD28937	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28937_Mjap_Gib2022_COI	ADL-B17	1	BD28937_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28937	Sample	BD28937	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B17	COI	45	BD28937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B17	AATTTAATTGTGACTAGGCATGGTCTG	138.50901
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28938_Cmae_Bot2011_COI	ADL-B16	1	BD28938_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	ATGAACAGTCTATCCTCCTTTAG	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28938_Hsan_Wil2021_COI	ADL-B16	1	BD28938_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	CCTGGGCCGGTATAGTAGGT	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28938_Mros_Gib2022_COI	ADL-B16	1	BD28938_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28938_Msal_Bot2012_COI	ADL-B16	1	BD28938_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	GYTAGTTCCRATGATGTTAGCTG	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28938_Mstr_Wil2021_COI	ADL-B16	1	BD28938_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	GTAGTGGTAACTACTCATGCTTTA	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28938_Pvir_Dia2013_COI	ADL-B16	1	BD28938_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	CTTAGTGGCATTAATTCGDAATCC	138.50756
BD28938	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82669	WGS84	BD28938	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28938_Rhar_Sim2018_COI	ADL-B16	1	BD28938_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28938	Sample	BD28938	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B16	COI	45	BD28938	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B16	CTGCTGCTATTGCTCATG	138.50756
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28940_Cjap_Sim2018_COI	ADL-B14	1	BD28940_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28940_Esin_And2018_Cyb	ADL-B14	1	BD28940_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B14	Cytb	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	ACCCCTCCTCATATCCAACCA	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	31.488	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28940_Mgig_Bot2012_COI	ADL-B14	1	BD28940_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	TCTTATTCGTTGGAGACTTTATAACCCT	138.50873
BD28940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82861	WGS84	BD28940	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28940_Mjap_Gib2022_COI	ADL-B14	1	BD28940_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28940	Sample	BD28940	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B14	COI	45	BD28940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B14	AATTTAATTGTGACTAGGCATGGTCTG	138.50873
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28941_Cmae_Bot2011_COI	ADL-B13	1	BD28941_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	ATGAACAGTCTATCCTCCTTTAG	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28941_Hsan_Wil2021_COI	ADL-B13	1	BD28941_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	CCTGGGCCGGTATAGTAGGT	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28941_Mros_Gib2022_COI	ADL-B13	1	BD28941_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28941_Msal_Bot2012_COI	ADL-B13	1	BD28941_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	GYTAGTTCCRATGATGTTAGCTG	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28941_Mstr_Wil2021_COI	ADL-B13	1	BD28941_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	GTAGTGGTAACTACTCATGCTTTA	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28941_Pvir_Dia2013_COI	ADL-B13	1	BD28941_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	CTTAGTGGCATTAATTCGDAATCC	138.50754
BD28941	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.82868	WGS84	BD28941	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28941_Rhar_Sim2018_COI	ADL-B13	1	BD28941_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28941	Sample	BD28941	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B13	COI	45	BD28941	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B13	CTGCTGCTATTGCTCATG	138.50754
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28943_Aamu_Bax2006_COI	ADL-B11	1	BD28943_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	GCACAACCGGGATCTTTACTTC	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28943_Pcan_Bot2011_IGS	ADL-B11	1	BD28943_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B11	IGS	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	CGTAATCCTCAGTACTGGCTA	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28943_Pper_Dia2013_COI	ADL-B11	1	BD28943_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	CTTAGTGGCATTAATTCGDAATCC	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.118	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28943_Sspa_Oph2007_COI	ADL-B11	1	BD28943_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	GAAGGCCTTCCGATGATC	138.50868
BD28943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83012	WGS84	BD28943	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28943_Upin_Bot2011_COI	ADL-B11	1	BD28943_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28943	Sample	BD28943	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B11	COI	45	BD28943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B11	CTTTAATTACAGCGTTTTTATTGTTGT	138.50868
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28944_Cjap_Sim2018_COI	ADL-B10	1	BD28944_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28944_Esin_And2018_Cyb	ADL-B10	1	BD28944_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B10	Cytb	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	ACCCCTCCTCATATCCAACCA	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	30.748	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28944_Mgig_Bot2012_COI	ADL-B10	1	BD28944_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	TCTTATTCGTTGGAGACTTTATAACCCT	138.50761
BD28944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83071	WGS84	BD28944	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28944_Mjap_Gib2022_COI	ADL-B10	1	BD28944_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28944	Sample	BD28944	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B10	COI	45	BD28944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B10	AATTTAATTGTGACTAGGCATGGTCTG	138.50761
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28945_Cmae_Bot2011_COI	ADL-B09	1	BD28945_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	ATGAACAGTCTATCCTCCTTTAG	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28945_Hsan_Wil2021_COI	ADL-B09	1	BD28945_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	CCTGGGCCGGTATAGTAGGT	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28945_Mros_Gib2022_COI	ADL-B09	1	BD28945_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28945_Msal_Bot2012_COI	ADL-B09	1	BD28945_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	GYTAGTTCCRATGATGTTAGCTG	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28945_Mstr_Wil2021_COI	ADL-B09	1	BD28945_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	GTAGTGGTAACTACTCATGCTTTA	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28945_Pvir_Dia2013_COI	ADL-B09	1	BD28945_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	CTTAGTGGCATTAATTCGDAATCC	138.50754
BD28945	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83271	WGS84	BD28945	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28945_Rhar_Sim2018_COI	ADL-B09	1	BD28945_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28945	Sample	BD28945	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B09	COI	45	BD28945	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B09	CTGCTGCTATTGCTCATG	138.50754
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28947_Aamu_Bax2006_COI	ADL-B07	1	BD28947_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	GCACAACCGGGATCTTTACTTC	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28947_Pcan_Bot2011_IGS	ADL-B07	1	BD28947_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B07	IGS	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	CGTAATCCTCAGTACTGGCTA	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28947_Pper_Dia2013_COI	ADL-B07	1	BD28947_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	CTTAGTGGCATTAATTCGDAATCC	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28947_Sspa_Oph2007_COI	ADL-B07	1	BD28947_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	GAAGGCCTTCCGATGATC	138.50757
BD28947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83624	WGS84	BD28947	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28947_Upin_Bot2011_COI	ADL-B07	1	BD28947_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28947	Sample	BD28947	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B07	COI	45	BD28947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B07	CTTTAATTACAGCGTTTTTATTGTTGT	138.50757
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28948_Cjap_Sim2018_COI	ADL-B06	1	BD28948_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28948_Esin_And2018_Cyb	ADL-B06	1	BD28948_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B06	Cytb	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	ACCCCTCCTCATATCCAACCA	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.592	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28948_Mgig_Bot2012_COI	ADL-B06	1	BD28948_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	TCTTATTCGTTGGAGACTTTATAACCCT	138.50663
BD28948	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83888	WGS84	BD28948	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28948_Mjap_Gib2022_COI	ADL-B06	1	BD28948_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28948	Sample	BD28948	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B06	COI	45	BD28948	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B06	AATTTAATTGTGACTAGGCATGGTCTG	138.50663
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28949_Cmae_Bot2011_COI	ADL-B05	1	BD28949_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	ATGAACAGTCTATCCTCCTTTAG	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28949_Hsan_Wil2021_COI	ADL-B05	1	BD28949_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	CCTGGGCCGGTATAGTAGGT	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28949_Mros_Gib2022_COI	ADL-B05	1	BD28949_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28949_Msal_Bot2012_COI	ADL-B05	1	BD28949_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	GYTAGTTCCRATGATGTTAGCTG	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28949_Mstr_Wil2021_COI	ADL-B05	1	BD28949_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	GTAGTGGTAACTACTCATGCTTTA	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28949_Pvir_Dia2013_COI	ADL-B05	1	BD28949_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	CTTAGTGGCATTAATTCGDAATCC	138.50549
BD28949	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.83926	WGS84	BD28949	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28949_Rhar_Sim2018_COI	ADL-B05	1	BD28949_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28949	Sample	BD28949	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B05	COI	45	BD28949	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B05	CTGCTGCTATTGCTCATG	138.50549
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28950_Aamu_Bax2006_COI	ADL-B04	1	BD28950_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	GCACAACCGGGATCTTTACTTC	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28950_Pcan_Bot2011_IGS	ADL-B04	1	BD28950_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B04	IGS	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	CGTAATCCTCAGTACTGGCTA	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28950_Pper_Dia2013_COI	ADL-B04	1	BD28950_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	CTTAGTGGCATTAATTCGDAATCC	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28950_Sspa_Oph2007_COI	ADL-B04	1	BD28950_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	GAAGGCCTTCCGATGATC	138.50603
BD28950	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84	WGS84	BD28950	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28950_Upin_Bot2011_COI	ADL-B04	1	BD28950_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28950	Sample	BD28950	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B04	COI	45	BD28950	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B04	CTTTAATTACAGCGTTTTTATTGTTGT	138.50603
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28951_Cjap_Sim2018_COI	ADL-B03	1	BD28951_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28951_Esin_And2018_Cyb	ADL-B03	1	BD28951_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B03	Cytb	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	ACCCCTCCTCATATCCAACCA	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.238	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28951_Mgig_Bot2012_COI	ADL-B03	1	BD28951_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	TCTTATTCGTTGGAGACTTTATAACCCT	138.50558
BD28951	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84127	WGS84	BD28951	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28951_Mjap_Gib2022_COI	ADL-B03	1	BD28951_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28951	Sample	BD28951	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B03	COI	45	BD28951	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B03	AATTTAATTGTGACTAGGCATGGTCTG	138.50558
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28952_Cmae_Bot2011_COI	ADL-B02	1	BD28952_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	ATGAACAGTCTATCCTCCTTTAG	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28952_Hsan_Wil2021_COI	ADL-B02	1	BD28952_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	CCTGGGCCGGTATAGTAGGT	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28952_Mros_Gib2022_COI	ADL-B02	1	BD28952_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28952_Msal_Bot2012_COI	ADL-B02	1	BD28952_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	GYTAGTTCCRATGATGTTAGCTG	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28952_Mstr_Wil2021_COI	ADL-B02	1	BD28952_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	GTAGTGGTAACTACTCATGCTTTA	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28952_Pvir_Dia2013_COI	ADL-B02	1	BD28952_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	CTTAGTGGCATTAATTCGDAATCC	138.50066
BD28952	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.84244	WGS84	BD28952	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Summer	17/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28952_Rhar_Sim2018_COI	ADL-B02	1	BD28952_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28952	Sample	BD28952	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B02	COI	45	BD28952	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B02	CTGCTGCTATTGCTCATG	138.50066
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28954_Aamu_Bax2006_COI	ADL-B70	1	BD28954_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	GCACAACCGGGATCTTTACTTC	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28954_Pcan_Bot2011_IGS	ADL-B70	1	BD28954_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B70	IGS	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	CGTAATCCTCAGTACTGGCTA	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28954_Pper_Dia2013_COI	ADL-B70	1	BD28954_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	CTTAGTGGCATTAATTCGDAATCC	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.919	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28954_Sspa_Oph2007_COI	ADL-B70	1	BD28954_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	GAAGGCCTTCCGATGATC	138.5006
BD28954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76082	WGS84	BD28954	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28954_Upin_Bot2011_COI	ADL-B70	1	BD28954_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28954	Sample	BD28954	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B70	COI	45	BD28954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B70	CTTTAATTACAGCGTTTTTATTGTTGT	138.5006
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28955_Cjap_Sim2018_COI	ADL-B69	1	BD28955_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28955_Esin_And2018_Cyb	ADL-B69	1	BD28955_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B69	Cytb	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	ACCCCTCCTCATATCCAACCA	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	32.061	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28955_Mgig_Bot2012_COI	ADL-B69	1	BD28955_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	TCTTATTCGTTGGAGACTTTATAACCCT	138.49891
BD28955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76084	WGS84	BD28955	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28955_Mjap_Gib2022_COI	ADL-B69	1	BD28955_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28955	Sample	BD28955	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B69	COI	45	BD28955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B69	AATTTAATTGTGACTAGGCATGGTCTG	138.49891
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28956_Cmae_Bot2011_COI	ADL-B68	1	BD28956_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	ATGAACAGTCTATCCTCCTTTAG	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28956_Hsan_Wil2021_COI	ADL-B68	1	BD28956_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	CCTGGGCCGGTATAGTAGGT	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28956_Mros_Gib2022_COI	ADL-B68	1	BD28956_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28956_Msal_Bot2012_COI	ADL-B68	1	BD28956_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	GYTAGTTCCRATGATGTTAGCTG	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28956_Mstr_Wil2021_COI	ADL-B68	1	BD28956_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	GTAGTGGTAACTACTCATGCTTTA	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28956_Pvir_Dia2013_COI	ADL-B68	1	BD28956_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	CTTAGTGGCATTAATTCGDAATCC	138.50173
BD28956	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76224	WGS84	BD28956	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28956_Rhar_Sim2018_COI	ADL-B68	1	BD28956_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28956	Sample	BD28956	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B68	COI	45	BD28956	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B68	CTGCTGCTATTGCTCATG	138.50173
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28958_Aamu_Bax2006_COI	ADL-B66	1	BD28958_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	GCACAACCGGGATCTTTACTTC	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28958_Pcan_Bot2011_IGS	ADL-B66	1	BD28958_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B66	IGS	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	CGTAATCCTCAGTACTGGCTA	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28958_Pper_Dia2013_COI	ADL-B66	1	BD28958_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	CTTAGTGGCATTAATTCGDAATCC	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28958_Sspa_Oph2007_COI	ADL-B66	1	BD28958_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	GAAGGCCTTCCGATGATC	138.49752
BD28958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76233	WGS84	BD28958	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28958_Upin_Bot2011_COI	ADL-B66	1	BD28958_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28958	Sample	BD28958	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B66	COI	45	BD28958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B66	CTTTAATTACAGCGTTTTTATTGTTGT	138.49752
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28959_Cjap_Sim2018_COI	ADL-B65	1	BD28959_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28959_Esin_And2018_Cyb	ADL-B65	1	BD28959_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B65	Cytb	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	ACCCCTCCTCATATCCAACCA	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.665	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28959_Mgig_Bot2012_COI	ADL-B65	1	BD28959_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	TCTTATTCGTTGGAGACTTTATAACCCT	138.49536
BD28959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76234	WGS84	BD28959	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28959_Mjap_Gib2022_COI	ADL-B65	1	BD28959_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28959	Sample	BD28959	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B65	COI	45	BD28959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B65	AATTTAATTGTGACTAGGCATGGTCTG	138.49536
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28961_Aamu_Bax2006_COI	ADL-B63	1	BD28961_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	GCACAACCGGGATCTTTACTTC	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28961_Pcan_Bot2011_IGS	ADL-B63	1	BD28961_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B63	IGS	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	CGTAATCCTCAGTACTGGCTA	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28961_Pper_Dia2013_COI	ADL-B63	1	BD28961_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	CTTAGTGGCATTAATTCGDAATCC	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28961_Sspa_Oph2007_COI	ADL-B63	1	BD28961_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	GAAGGCCTTCCGATGATC	138.49468
BD28961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28961	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28961_Upin_Bot2011_COI	ADL-B63	1	BD28961_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28961	Sample	BD28961	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B63	COI	45	BD28961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B63	CTTTAATTACAGCGTTTTTATTGTTGT	138.49468
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28962_Cjap_Sim2018_COI	ADL-B62	1	BD28962_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28962_Esin_And2018_Cyb	ADL-B62	1	BD28962_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B62	Cytb	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	ACCCCTCCTCATATCCAACCA	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.705	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28962_Mgig_Bot2012_COI	ADL-B62	1	BD28962_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	TCTTATTCGTTGGAGACTTTATAACCCT	138.49227
BD28962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76396	WGS84	BD28962	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28962_Mjap_Gib2022_COI	ADL-B62	1	BD28962_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28962	Sample	BD28962	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B62	COI	45	BD28962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B62	AATTTAATTGTGACTAGGCATGGTCTG	138.49227
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28963_Cmae_Bot2011_COI	ADL-B61	1	BD28963_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	ATGAACAGTCTATCCTCCTTTAG	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28963_Hsan_Wil2021_COI	ADL-B61	1	BD28963_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	CCTGGGCCGGTATAGTAGGT	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28963_Mros_Gib2022_COI	ADL-B61	1	BD28963_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28963_Msal_Bot2012_COI	ADL-B61	1	BD28963_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	GYTAGTTCCRATGATGTTAGCTG	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28963_Mstr_Wil2021_COI	ADL-B61	1	BD28963_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	GTAGTGGTAACTACTCATGCTTTA	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28963_Pvir_Dia2013_COI	ADL-B61	1	BD28963_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	CTTAGTGGCATTAATTCGDAATCC	138.49346
BD28963	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76548	WGS84	BD28963	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28963_Rhar_Sim2018_COI	ADL-B61	1	BD28963_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28963	Sample	BD28963	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B61	COI	45	BD28963	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B61	CTGCTGCTATTGCTCATG	138.49346
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28965_Aamu_Bax2006_COI	ADL-B59	1	BD28965_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	GCACAACCGGGATCTTTACTTC	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28965_Pcan_Bot2011_IGS	ADL-B59	1	BD28965_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B59	IGS	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	CGTAATCCTCAGTACTGGCTA	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28965_Pper_Dia2013_COI	ADL-B59	1	BD28965_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	CTTAGTGGCATTAATTCGDAATCC	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28965_Sspa_Oph2007_COI	ADL-B59	1	BD28965_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	GAAGGCCTTCCGATGATC	138.49026
BD28965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76708	WGS84	BD28965	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28965_Upin_Bot2011_COI	ADL-B59	1	BD28965_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28965	Sample	BD28965	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B59	COI	45	BD28965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B59	CTTTAATTACAGCGTTTTTATTGTTGT	138.49026
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28966_Cjap_Sim2018_COI	ADL-B58	1	BD28966_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28966_Esin_And2018_Cyb	ADL-B58	1	BD28966_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B58	Cytb	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	ACCCCTCCTCATATCCAACCA	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.967	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28966_Mgig_Bot2012_COI	ADL-B58	1	BD28966_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	TCTTATTCGTTGGAGACTTTATAACCCT	138.48822
BD28966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76714	WGS84	BD28966	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28966_Mjap_Gib2022_COI	ADL-B58	1	BD28966_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28966	Sample	BD28966	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B58	COI	45	BD28966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B58	AATTTAATTGTGACTAGGCATGGTCTG	138.48822
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28967_Cmae_Bot2011_COI	ADL-B57	1	BD28967_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	ATGAACAGTCTATCCTCCTTTAG	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28967_Hsan_Wil2021_COI	ADL-B57	1	BD28967_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	CCTGGGCCGGTATAGTAGGT	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28967_Mros_Gib2022_COI	ADL-B57	1	BD28967_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28967_Msal_Bot2012_COI	ADL-B57	1	BD28967_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	GYTAGTTCCRATGATGTTAGCTG	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28967_Mstr_Wil2021_COI	ADL-B57	1	BD28967_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	GTAGTGGTAACTACTCATGCTTTA	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28967_Pvir_Dia2013_COI	ADL-B57	1	BD28967_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	CTTAGTGGCATTAATTCGDAATCC	138.48711
BD28967	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76563	WGS84	BD28967	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28967_Rhar_Sim2018_COI	ADL-B57	1	BD28967_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28967	Sample	BD28967	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B57	COI	45	BD28967	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B57	CTGCTGCTATTGCTCATG	138.48711
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28969_Aamu_Bax2006_COI	ADL-B55	1	BD28969_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	GCACAACCGGGATCTTTACTTC	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28969_Pcan_Bot2011_IGS	ADL-B55	1	BD28969_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B55	IGS	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	CGTAATCCTCAGTACTGGCTA	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28969_Pper_Dia2013_COI	ADL-B55	1	BD28969_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	CTTAGTGGCATTAATTCGDAATCC	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.993	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28969_Sspa_Oph2007_COI	ADL-B55	1	BD28969_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	GAAGGCCTTCCGATGATC	138.48937
BD28969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76855	WGS84	BD28969	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28969_Upin_Bot2011_COI	ADL-B55	1	BD28969_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28969	Sample	BD28969	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B55	COI	45	BD28969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B55	CTTTAATTACAGCGTTTTTATTGTTGT	138.48937
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28970_Cmae_Bot2011_COI	ADL-B54	1	BD28970_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	ATGAACAGTCTATCCTCCTTTAG	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28970_Hsan_Wil2021_COI	ADL-B54	1	BD28970_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	CCTGGGCCGGTATAGTAGGT	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28970_Mros_Gib2022_COI	ADL-B54	1	BD28970_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28970_Msal_Bot2012_COI	ADL-B54	1	BD28970_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	GYTAGTTCCRATGATGTTAGCTG	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28970_Mstr_Wil2021_COI	ADL-B54	1	BD28970_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	GTAGTGGTAACTACTCATGCTTTA	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28970_Pvir_Dia2013_COI	ADL-B54	1	BD28970_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	CTTAGTGGCATTAATTCGDAATCC	138.48724
BD28970	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.76866	WGS84	BD28970	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28970_Rhar_Sim2018_COI	ADL-B54	1	BD28970_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28970	Sample	BD28970	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B54	COI	45	BD28970	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B54	CTGCTGCTATTGCTCATG	138.48724
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28972_Aamu_Bax2006_COI	ADL-B52	1	BD28972_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	GCACAACCGGGATCTTTACTTC	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28972_Pcan_Bot2011_IGS	ADL-B52	1	BD28972_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B52	IGS	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	CGTAATCCTCAGTACTGGCTA	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28972_Pper_Dia2013_COI	ADL-B52	1	BD28972_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	CTTAGTGGCATTAATTCGDAATCC	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28972_Sspa_Oph2007_COI	ADL-B52	1	BD28972_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	GAAGGCCTTCCGATGATC	138.4862
BD28972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77014	WGS84	BD28972	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28972_Upin_Bot2011_COI	ADL-B52	1	BD28972_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28972	Sample	BD28972	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B52	COI	45	BD28972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B52	CTTTAATTACAGCGTTTTTATTGTTGT	138.4862
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28973_Cjap_Sim2018_COI	ADL-B51	1	BD28973_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28973_Esin_And2018_Cyb	ADL-B51	1	BD28973_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B51	Cytb	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	ACCCCTCCTCATATCCAACCA	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28973_Mgig_Bot2012_COI	ADL-B51	1	BD28973_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	TCTTATTCGTTGGAGACTTTATAACCCT	138.48404
BD28973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77031	WGS84	BD28973	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28973_Mjap_Gib2022_COI	ADL-B51	1	BD28973_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28973	Sample	BD28973	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B51	COI	45	BD28973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B51	AATTTAATTGTGACTAGGCATGGTCTG	138.48404
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28974_Cmae_Bot2011_COI	ADL-B50	1	BD28974_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	ATGAACAGTCTATCCTCCTTTAG	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28974_Hsan_Wil2021_COI	ADL-B50	1	BD28974_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	CCTGGGCCGGTATAGTAGGT	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28974_Mros_Gib2022_COI	ADL-B50	1	BD28974_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28974_Msal_Bot2012_COI	ADL-B50	1	BD28974_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	GYTAGTTCCRATGATGTTAGCTG	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28974_Mstr_Wil2021_COI	ADL-B50	1	BD28974_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	GTAGTGGTAACTACTCATGCTTTA	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28974_Pvir_Dia2013_COI	ADL-B50	1	BD28974_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	CTTAGTGGCATTAATTCGDAATCC	138.48525
BD28974	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77169	WGS84	BD28974	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28974_Rhar_Sim2018_COI	ADL-B50	1	BD28974_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28974	Sample	BD28974	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B50	COI	45	BD28974	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B50	CTGCTGCTATTGCTCATG	138.48525
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28976_Aamu_Bax2006_COI	ADL-B48	1	BD28976_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	GCACAACCGGGATCTTTACTTC	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28976_Pcan_Bot2011_IGS	ADL-B48	1	BD28976_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B48	IGS	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	CGTAATCCTCAGTACTGGCTA	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28976_Pper_Dia2013_COI	ADL-B48	1	BD28976_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	CTTAGTGGCATTAATTCGDAATCC	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.903	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28976_Sspa_Oph2007_COI	ADL-B48	1	BD28976_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	GAAGGCCTTCCGATGATC	138.48421
BD28976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7732	WGS84	BD28976	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28976_Upin_Bot2011_COI	ADL-B48	1	BD28976_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28976	Sample	BD28976	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B48	COI	45	BD28976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B48	CTTTAATTACAGCGTTTTTATTGTTGT	138.48421
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28977_Cjap_Sim2018_COI	ADL-B47	1	BD28977_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28977_Esin_And2018_Cyb	ADL-B47	1	BD28977_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B47	Cytb	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	ACCCCTCCTCATATCCAACCA	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.744	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28977_Mgig_Bot2012_COI	ADL-B47	1	BD28977_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	TCTTATTCGTTGGAGACTTTATAACCCT	138.48203
BD28977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77323	WGS84	BD28977	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28977_Mjap_Gib2022_COI	ADL-B47	1	BD28977_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28977	Sample	BD28977	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B47	COI	45	BD28977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B47	AATTTAATTGTGACTAGGCATGGTCTG	138.48203
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28978_Cmae_Bot2011_COI	ADL-B46	1	BD28978_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	ATGAACAGTCTATCCTCCTTTAG	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28978_Hsan_Wil2021_COI	ADL-B46	1	BD28978_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	CCTGGGCCGGTATAGTAGGT	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28978_Mros_Gib2022_COI	ADL-B46	1	BD28978_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28978_Msal_Bot2012_COI	ADL-B46	1	BD28978_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	GYTAGTTCCRATGATGTTAGCTG	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28978_Mstr_Wil2021_COI	ADL-B46	1	BD28978_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	GTAGTGGTAACTACTCATGCTTTA	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28978_Pvir_Dia2013_COI	ADL-B46	1	BD28978_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	CTTAGTGGCATTAATTCGDAATCC	138.48334
BD28978	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77474	WGS84	BD28978	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28978_Rhar_Sim2018_COI	ADL-B46	1	BD28978_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28978	Sample	BD28978	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B46	COI	45	BD28978	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B46	CTGCTGCTATTGCTCATG	138.48334
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.77483	WGS84	BD28980	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28980_Asen_Wil2025_COI	ADL-B44	1	BD28980_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	GTTGTTGTTACAACACATGCTCTTTTG	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28980_Cjap_Sim2018_COI	ADL-B44	1	BD28980_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28980_Esin_And2018_Cyb	ADL-B44	1	BD28980_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B44	Cytb	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	ACCCCTCCTCATATCCAACCA	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28980_Mgig_Bot2012_COI	ADL-B44	1	BD28980_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	TCTTATTCGTTGGAGACTTTATAACCCT	138.48112
BD28980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77483	WGS84	BD28980	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28980_Mjap_Gib2022_COI	ADL-B44	1	BD28980_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28980	Sample	BD28980	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B44	COI	45	BD28980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B44	AATTTAATTGTGACTAGGCATGGTCTG	138.48112
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28981_Cmae_Bot2011_COI	ADL-B43	1	BD28981_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	ATGAACAGTCTATCCTCCTTTAG	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28981_Hsan_Wil2021_COI	ADL-B43	1	BD28981_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	CCTGGGCCGGTATAGTAGGT	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28981_Mros_Gib2022_COI	ADL-B43	1	BD28981_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28981_Msal_Bot2012_COI	ADL-B43	1	BD28981_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	GYTAGTTCCRATGATGTTAGCTG	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28981_Mstr_Wil2021_COI	ADL-B43	1	BD28981_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	GTAGTGGTAACTACTCATGCTTTA	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28981_Pvir_Dia2013_COI	ADL-B43	1	BD28981_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	CTTAGTGGCATTAATTCGDAATCC	138.4802
BD28981	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.77623	WGS84	BD28981	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28981_Rhar_Sim2018_COI	ADL-B43	1	BD28981_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28981	Sample	BD28981	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B43	COI	45	BD28981	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B43	CTGCTGCTATTGCTCATG	138.4802
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28983_Aamu_Bax2006_COI	ADL-B41	1	BD28983_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	GCACAACCGGGATCTTTACTTC	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28983_Pcan_Bot2011_IGS	ADL-B41	1	BD28983_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B41	IGS	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	CGTAATCCTCAGTACTGGCTA	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28983_Pper_Dia2013_COI	ADL-B41	1	BD28983_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	CTTAGTGGCATTAATTCGDAATCC	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.296	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28983_Sspa_Oph2007_COI	ADL-B41	1	BD28983_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	GAAGGCCTTCCGATGATC	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.7795	WGS84	BD28983	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28983_Upin_Bot2011_COI	ADL-B41	1	BD28983_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	CTTTAATTACAGCGTTTTTATTGTTGT	138.47823
BD28983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.7795	WGS84	BD28983	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28983_Vgib_Wil2025_COI	ADL-B41	1	BD28983_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28983	Sample	BD28983	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B41	COI	45	BD28983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B41	CCCCTCTTTACAAGTTCTTTAGGGT	138.47823
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78099	WGS84	BD28984	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28984_Asen_Wil2025_COI	ADL-B40	1	BD28984_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	GTTGTTGTTACAACACATGCTCTTTTG	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28984_Cjap_Sim2018_COI	ADL-B40	1	BD28984_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28984_Esin_And2018_Cyb	ADL-B40	1	BD28984_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B40	Cytb	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	ACCCCTCCTCATATCCAACCA	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28984_Mgig_Bot2012_COI	ADL-B40	1	BD28984_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	TCTTATTCGTTGGAGACTTTATAACCCT	138.47714
BD28984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78099	WGS84	BD28984	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28984_Mjap_Gib2022_COI	ADL-B40	1	BD28984_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28984	Sample	BD28984	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B40	COI	45	BD28984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B40	AATTTAATTGTGACTAGGCATGGTCTG	138.47714
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28985_Cmae_Bot2011_COI	ADL-B39	1	BD28985_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	ATGAACAGTCTATCCTCCTTTAG	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28985_Hsan_Wil2021_COI	ADL-B39	1	BD28985_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	CCTGGGCCGGTATAGTAGGT	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28985_Mros_Gib2022_COI	ADL-B39	1	BD28985_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28985_Msal_Bot2012_COI	ADL-B39	1	BD28985_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	GYTAGTTCCRATGATGTTAGCTG	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28985_Mstr_Wil2021_COI	ADL-B39	1	BD28985_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	GTAGTGGTAACTACTCATGCTTTA	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28985_Pvir_Dia2013_COI	ADL-B39	1	BD28985_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	CTTAGTGGCATTAATTCGDAATCC	138.47624
BD28985	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78257	WGS84	BD28985	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28985_Rhar_Sim2018_COI	ADL-B39	1	BD28985_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28985	Sample	BD28985	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-B39	COI	45	BD28985	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B39	CTGCTGCTATTGCTCATG	138.47624
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28987_Aamu_Bax2006_COI	ADL-B37	1	BD28987_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	GCACAACCGGGATCTTTACTTC	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28987_Pcan_Bot2011_IGS	ADL-B37	1	BD28987_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-B37	IGS	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	CGTAATCCTCAGTACTGGCTA	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28987_Pper_Dia2013_COI	ADL-B37	1	BD28987_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	CTTAGTGGCATTAATTCGDAATCC	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28987_Sspa_Oph2007_COI	ADL-B37	1	BD28987_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	GAAGGCCTTCCGATGATC	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78416	WGS84	BD28987	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28987_Upin_Bot2011_COI	ADL-B37	1	BD28987_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	CTTTAATTACAGCGTTTTTATTGTTGT	138.47512
BD28987	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78416	WGS84	BD28987	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD28987_Vgib_Wil2025_COI	ADL-B37	1	BD28987_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28987	Sample	BD28987	targeted	Absent	Sample	Vgib_Wil2025_COI	ADL-B37	COI	45	BD28987	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B37	CCCCTCTTTACAAGTTCTTTAGGGT	138.47512
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.78394	WGS84	BD28988	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD28988_Asen_Wil2025_COI	ADL-B36	1	BD28988_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Asen_Wil2025_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	GTTGTTGTTACAACACATGCTCTTTTG	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28988_Cjap_Sim2018_COI	ADL-B36	1	BD28988_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28988_Esin_And2018_Cyb	ADL-B36	1	BD28988_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-B36	Cytb	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	ACCCCTCCTCATATCCAACCA	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28988_Mgig_Bot2012_COI	ADL-B36	1	BD28988_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	TCTTATTCGTTGGAGACTTTATAACCCT	138.47309
BD28988	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14709	-34.78394	WGS84	BD28988	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Summer	3/10/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28988_Mjap_Gib2022_COI	ADL-B36	1	BD28988_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28988	Sample	BD28988	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-B36	COI	45	BD28988	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-B36	AATTTAATTGTGACTAGGCATGGTCTG	138.47309
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28989_Cmae_Bot2011_COI	PLO-B40	1	BD28989_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	ATGAACAGTCTATCCTCCTTTAG	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28989_Hsan_Wil2021_COI	PLO-B40	1	BD28989_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	CCTGGGCCGGTATAGTAGGT	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28989_Mros_Gib2022_COI	PLO-B40	1	BD28989_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28989_Msal_Bot2012_COI	PLO-B40	1	BD28989_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	GYTAGTTCCRATGATGTTAGCTG	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28989_Mstr_Wil2021_COI	PLO-B40	1	BD28989_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	GTAGTGGTAACTACTCATGCTTTA	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28989_Pvir_Dia2013_COI	PLO-B40	1	BD28989_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	CTTAGTGGCATTAATTCGDAATCC	135.86778
BD28989	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74	WGS84	BD28989	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28989_Rhar_Sim2018_COI	PLO-B40	1	BD28989_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28989	Sample	BD28989	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B40	COI	45	BD28989	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B40	CTGCTGCTATTGCTCATG	135.86778
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28990_Aamu_Bax2006_COI	PLO-B39	1	BD28990_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	GCACAACCGGGATCTTTACTTC	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28990_Pcan_Bot2011_IGS	PLO-B39	1	BD28990_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B39	IGS	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	CGTAATCCTCAGTACTGGCTA	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28990_Pper_Dia2013_COI	PLO-B39	1	BD28990_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	CTTAGTGGCATTAATTCGDAATCC	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.374	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28990_Sspa_Oph2007_COI	PLO-B39	1	BD28990_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	GAAGGCCTTCCGATGATC	135.86996
BD28990	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73963	WGS84	BD28990	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28990_Upin_Bot2011_COI	PLO-B39	1	BD28990_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28990	Sample	BD28990	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B39	COI	45	BD28990	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B39	CTTTAATTACAGCGTTTTTATTGTTGT	135.86996
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28991_Cjap_Sim2018_COI	PLO-B38	1	BD28991_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28991_Esin_And2018_Cyb	PLO-B38	1	BD28991_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B38	Cytb	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	ACCCCTCCTCATATCCAACCA	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28991_Mgig_Bot2012_COI	PLO-B38	1	BD28991_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	TCTTATTCGTTGGAGACTTTATAACCCT	135.87269
BD28991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.73937	WGS84	BD28991	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28991_Mjap_Gib2022_COI	PLO-B38	1	BD28991_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28991	Sample	BD28991	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B38	COI	45	BD28991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B38	AATTTAATTGTGACTAGGCATGGTCTG	135.87269
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28992_Cmae_Bot2011_COI	PLO-B37	1	BD28992_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	ATGAACAGTCTATCCTCCTTTAG	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28992_Hsan_Wil2021_COI	PLO-B37	1	BD28992_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	CCTGGGCCGGTATAGTAGGT	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28992_Mros_Gib2022_COI	PLO-B37	1	BD28992_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28992_Msal_Bot2012_COI	PLO-B37	1	BD28992_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	GYTAGTTCCRATGATGTTAGCTG	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28992_Mstr_Wil2021_COI	PLO-B37	1	BD28992_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	GTAGTGGTAACTACTCATGCTTTA	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28992_Pvir_Dia2013_COI	PLO-B37	1	BD28992_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	CTTAGTGGCATTAATTCGDAATCC	135.87379
BD28992	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.74189	WGS84	BD28992	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28992_Rhar_Sim2018_COI	PLO-B37	1	BD28992_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28992	Sample	BD28992	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B37	COI	45	BD28992	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B37	CTGCTGCTATTGCTCATG	135.87379
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28994_Aamu_Bax2006_COI	THE-B35	1	BD28994_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	GCACAACCGGGATCTTTACTTC	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28994_Pcan_Bot2011_IGS	THE-B35	1	BD28994_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B35	IGS	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	CGTAATCCTCAGTACTGGCTA	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28994_Pper_Dia2013_COI	THE-B35	1	BD28994_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	CTTAGTGGCATTAATTCGDAATCC	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28994_Sspa_Oph2007_COI	THE-B35	1	BD28994_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	GAAGGCCTTCCGATGATC	133.63829
BD28994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14554	WGS84	BD28994	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28994_Upin_Bot2011_COI	THE-B35	1	BD28994_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28994	Sample	BD28994	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B35	COI	45	BD28994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B35	CTTTAATTACAGCGTTTTTATTGTTGT	133.63829
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28995_Cjap_Sim2018_COI	THE-B34	1	BD28995_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28995_Esin_And2018_Cyb	THE-B34	1	BD28995_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B34	Cytb	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	ACCCCTCCTCATATCCAACCA	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.467	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28995_Mgig_Bot2012_COI	THE-B34	1	BD28995_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	TCTTATTCGTTGGAGACTTTATAACCCT	133.63995
BD28995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14572	WGS84	BD28995	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28995_Mjap_Gib2022_COI	THE-B34	1	BD28995_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28995	Sample	BD28995	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B34	COI	45	BD28995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B34	AATTTAATTGTGACTAGGCATGGTCTG	133.63995
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD28996_Cmae_Bot2011_COI	THE-B33	1	BD28996_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	ATGAACAGTCTATCCTCCTTTAG	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD28996_Hsan_Wil2021_COI	THE-B33	1	BD28996_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	CCTGGGCCGGTATAGTAGGT	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD28996_Mros_Gib2022_COI	THE-B33	1	BD28996_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD28996_Msal_Bot2012_COI	THE-B33	1	BD28996_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	GYTAGTTCCRATGATGTTAGCTG	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD28996_Mstr_Wil2021_COI	THE-B33	1	BD28996_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	GTAGTGGTAACTACTCATGCTTTA	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD28996_Pvir_Dia2013_COI	THE-B33	1	BD28996_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	CTTAGTGGCATTAATTCGDAATCC	133.64047
BD28996	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14539	WGS84	BD28996	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD28996_Rhar_Sim2018_COI	THE-B33	1	BD28996_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28996	Sample	BD28996	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B33	COI	45	BD28996	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B33	CTGCTGCTATTGCTCATG	133.64047
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD28998_Aamu_Bax2006_COI	THE-B31	1	BD28998_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	GCACAACCGGGATCTTTACTTC	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD28998_Pcan_Bot2011_IGS	THE-B31	1	BD28998_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B31	IGS	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	CGTAATCCTCAGTACTGGCTA	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD28998_Pper_Dia2013_COI	THE-B31	1	BD28998_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	CTTAGTGGCATTAATTCGDAATCC	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD28998_Sspa_Oph2007_COI	THE-B31	1	BD28998_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	GAAGGCCTTCCGATGATC	133.63652
BD28998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14676	WGS84	BD28998	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD28998_Upin_Bot2011_COI	THE-B31	1	BD28998_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28998	Sample	BD28998	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B31	COI	45	BD28998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B31	CTTTAATTACAGCGTTTTTATTGTTGT	133.63652
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD28999_Cjap_Sim2018_COI	THE-B30	1	BD28999_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD28999_Esin_And2018_Cyb	THE-B30	1	BD28999_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B30	Cytb	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	ACCCCTCCTCATATCCAACCA	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.884	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD28999_Mgig_Bot2012_COI	THE-B30	1	BD28999_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	TCTTATTCGTTGGAGACTTTATAACCCT	133.63797
BD28999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14686	WGS84	BD28999	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD28999_Mjap_Gib2022_COI	THE-B30	1	BD28999_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD28999	Sample	BD28999	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B30	COI	45	BD28999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B30	AATTTAATTGTGACTAGGCATGGTCTG	133.63797
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29001_Aamu_Bax2006_COI	THE-B28	1	BD29001_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	GCACAACCGGGATCTTTACTTC	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29001_Pcan_Bot2011_IGS	THE-B28	1	BD29001_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B28	IGS	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	CGTAATCCTCAGTACTGGCTA	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29001_Pper_Dia2013_COI	THE-B28	1	BD29001_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	CTTAGTGGCATTAATTCGDAATCC	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29001_Sspa_Oph2007_COI	THE-B28	1	BD29001_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	GAAGGCCTTCCGATGATC	133.63921
BD29001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14674	WGS84	BD29001	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29001_Upin_Bot2011_COI	THE-B28	1	BD29001_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29001	Sample	BD29001	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B28	COI	45	BD29001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B28	CTTTAATTACAGCGTTTTTATTGTTGT	133.63921
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29002_Cjap_Sim2018_COI	THE-B27	1	BD29002_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29002_Esin_And2018_Cyb	THE-B27	1	BD29002_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B27	Cytb	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	ACCCCTCCTCATATCCAACCA	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	33.407	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29002_Mgig_Bot2012_COI	THE-B27	1	BD29002_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	TCTTATTCGTTGGAGACTTTATAACCCT	133.64049
BD29002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14682	WGS84	BD29002	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29002_Mjap_Gib2022_COI	THE-B27	1	BD29002_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29002	Sample	BD29002	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B27	COI	45	BD29002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B27	AATTTAATTGTGACTAGGCATGGTCTG	133.64049
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29003_Cmae_Bot2011_COI	THE-B26	1	BD29003_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	ATGAACAGTCTATCCTCCTTTAG	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29003_Hsan_Wil2021_COI	THE-B26	1	BD29003_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	CCTGGGCCGGTATAGTAGGT	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29003_Mros_Gib2022_COI	THE-B26	1	BD29003_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29003_Msal_Bot2012_COI	THE-B26	1	BD29003_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	GYTAGTTCCRATGATGTTAGCTG	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29003_Mstr_Wil2021_COI	THE-B26	1	BD29003_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	GTAGTGGTAACTACTCATGCTTTA	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29003_Pvir_Dia2013_COI	THE-B26	1	BD29003_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	CTTAGTGGCATTAATTCGDAATCC	133.63596
BD29003	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14788	WGS84	BD29003	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29003_Rhar_Sim2018_COI	THE-B26	1	BD29003_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29003	Sample	BD29003	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B26	COI	45	BD29003	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B26	CTGCTGCTATTGCTCATG	133.63596
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29005_Aamu_Bax2006_COI	THE-B24	1	BD29005_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	GCACAACCGGGATCTTTACTTC	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29005_Pcan_Bot2011_IGS	THE-B24	1	BD29005_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B24	IGS	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	CGTAATCCTCAGTACTGGCTA	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29005_Pper_Dia2013_COI	THE-B24	1	BD29005_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	CTTAGTGGCATTAATTCGDAATCC	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29005_Sspa_Oph2007_COI	THE-B24	1	BD29005_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	GAAGGCCTTCCGATGATC	133.63886
BD29005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14776	WGS84	BD29005	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29005_Upin_Bot2011_COI	THE-B24	1	BD29005_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29005	Sample	BD29005	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B24	COI	45	BD29005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B24	CTTTAATTACAGCGTTTTTATTGTTGT	133.63886
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29006_Cjap_Sim2018_COI	THE-B23	1	BD29006_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29006_Esin_And2018_Cyb	THE-B23	1	BD29006_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B23	Cytb	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	ACCCCTCCTCATATCCAACCA	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	41.113	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29006_Mgig_Bot2012_COI	THE-B23	1	BD29006_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	TCTTATTCGTTGGAGACTTTATAACCCT	133.64018
BD29006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14777	WGS84	BD29006	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29006_Mjap_Gib2022_COI	THE-B23	1	BD29006_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29006	Sample	BD29006	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B23	COI	45	BD29006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B23	AATTTAATTGTGACTAGGCATGGTCTG	133.64018
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29007_Cmae_Bot2011_COI	THE-B22	1	BD29007_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	ATGAACAGTCTATCCTCCTTTAG	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29007_Hsan_Wil2021_COI	THE-B22	1	BD29007_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	CCTGGGCCGGTATAGTAGGT	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29007_Mros_Gib2022_COI	THE-B22	1	BD29007_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29007_Msal_Bot2012_COI	THE-B22	1	BD29007_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	GYTAGTTCCRATGATGTTAGCTG	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29007_Mstr_Wil2021_COI	THE-B22	1	BD29007_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	GTAGTGGTAACTACTCATGCTTTA	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29007_Pvir_Dia2013_COI	THE-B22	1	BD29007_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	CTTAGTGGCATTAATTCGDAATCC	133.64094
BD29007	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14784	WGS84	BD29007	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29007_Rhar_Sim2018_COI	THE-B22	1	BD29007_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29007	Sample	BD29007	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B22	COI	45	BD29007	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B22	CTGCTGCTATTGCTCATG	133.64094
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29009_Aamu_Bax2006_COI	THE-B20	1	BD29009_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	GCACAACCGGGATCTTTACTTC	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29009_Pcan_Bot2011_IGS	THE-B20	1	BD29009_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B20	IGS	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	CGTAATCCTCAGTACTGGCTA	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29009_Pper_Dia2013_COI	THE-B20	1	BD29009_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	CTTAGTGGCATTAATTCGDAATCC	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29009_Sspa_Oph2007_COI	THE-B20	1	BD29009_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	GAAGGCCTTCCGATGATC	133.63656
BD29009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14903	WGS84	BD29009	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29009_Upin_Bot2011_COI	THE-B20	1	BD29009_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29009	Sample	BD29009	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B20	COI	45	BD29009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B20	CTTTAATTACAGCGTTTTTATTGTTGT	133.63656
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29010_Cmae_Bot2011_COI	THE-B19	1	BD29010_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	ATGAACAGTCTATCCTCCTTTAG	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29010_Hsan_Wil2021_COI	THE-B19	1	BD29010_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	CCTGGGCCGGTATAGTAGGT	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29010_Mros_Gib2022_COI	THE-B19	1	BD29010_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29010_Msal_Bot2012_COI	THE-B19	1	BD29010_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	GYTAGTTCCRATGATGTTAGCTG	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29010_Mstr_Wil2021_COI	THE-B19	1	BD29010_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	GTAGTGGTAACTACTCATGCTTTA	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29010_Pvir_Dia2013_COI	THE-B19	1	BD29010_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	CTTAGTGGCATTAATTCGDAATCC	133.63803
BD29010	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14915	WGS84	BD29010	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29010_Rhar_Sim2018_COI	THE-B19	1	BD29010_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29010	Sample	BD29010	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B19	COI	45	BD29010	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B19	CTGCTGCTATTGCTCATG	133.63803
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29012_Aamu_Bax2006_COI	THE-B17	1	BD29012_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	GCACAACCGGGATCTTTACTTC	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29012_Pcan_Bot2011_IGS	THE-B17	1	BD29012_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B17	IGS	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	CGTAATCCTCAGTACTGGCTA	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29012_Pper_Dia2013_COI	THE-B17	1	BD29012_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	CTTAGTGGCATTAATTCGDAATCC	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29012_Sspa_Oph2007_COI	THE-B17	1	BD29012_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	GAAGGCCTTCCGATGATC	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15352	WGS84	BD29012	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29012_Upin_Bot2011_COI	THE-B17	1	BD29012_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	CTTTAATTACAGCGTTTTTATTGTTGT	133.63895
BD29012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15352	WGS84	BD29012	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29012_Vgib_Wil2025_COI	THE-B17	1	BD29012_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29012	Sample	BD29012	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B17	COI	45	BD29012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B17	CCCCTCTTTACAAGTTCTTTAGGGT	133.63895
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15244	WGS84	BD29013	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29013_Asen_Wil2025_COI	THE-B16	1	BD29013_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	GTTGTTGTTACAACACATGCTCTTTTG	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29013_Cjap_Sim2018_COI	THE-B16	1	BD29013_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29013_Esin_And2018_Cyb	THE-B16	1	BD29013_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B16	Cytb	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	ACCCCTCCTCATATCCAACCA	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.047	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29013_Mgig_Bot2012_COI	THE-B16	1	BD29013_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	TCTTATTCGTTGGAGACTTTATAACCCT	133.64047
BD29013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15244	WGS84	BD29013	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29013_Mjap_Gib2022_COI	THE-B16	1	BD29013_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29013	Sample	BD29013	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B16	COI	45	BD29013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B16	AATTTAATTGTGACTAGGCATGGTCTG	133.64047
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29014_Cmae_Bot2011_COI	THE-B15	1	BD29014_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	ATGAACAGTCTATCCTCCTTTAG	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29014_Hsan_Wil2021_COI	THE-B15	1	BD29014_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	CCTGGGCCGGTATAGTAGGT	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29014_Mros_Gib2022_COI	THE-B15	1	BD29014_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29014_Msal_Bot2012_COI	THE-B15	1	BD29014_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	GYTAGTTCCRATGATGTTAGCTG	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29014_Mstr_Wil2021_COI	THE-B15	1	BD29014_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	GTAGTGGTAACTACTCATGCTTTA	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29014_Pvir_Dia2013_COI	THE-B15	1	BD29014_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	CTTAGTGGCATTAATTCGDAATCC	133.63988
BD29014	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15215	WGS84	BD29014	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29014_Rhar_Sim2018_COI	THE-B15	1	BD29014_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29014	Sample	BD29014	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B15	COI	45	BD29014	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B15	CTGCTGCTATTGCTCATG	133.63988
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29016_Aamu_Bax2006_COI	THE-B13	1	BD29016_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	GCACAACCGGGATCTTTACTTC	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29016_Pcan_Bot2011_IGS	THE-B13	1	BD29016_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B13	IGS	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	CGTAATCCTCAGTACTGGCTA	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29016_Pper_Dia2013_COI	THE-B13	1	BD29016_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	CTTAGTGGCATTAATTCGDAATCC	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29016_Sspa_Oph2007_COI	THE-B13	1	BD29016_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	GAAGGCCTTCCGATGATC	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15214	WGS84	BD29016	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29016_Upin_Bot2011_COI	THE-B13	1	BD29016_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	CTTTAATTACAGCGTTTTTATTGTTGT	133.63703
BD29016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15214	WGS84	BD29016	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29016_Vgib_Wil2025_COI	THE-B13	1	BD29016_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29016	Sample	BD29016	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B13	COI	45	BD29016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B13	CCCCTCTTTACAAGTTCTTTAGGGT	133.63703
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15206	WGS84	BD29017	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29017_Asen_Wil2025_COI	THE-B12	1	BD29017_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	GTTGTTGTTACAACACATGCTCTTTTG	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29017_Cjap_Sim2018_COI	THE-B12	1	BD29017_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29017_Esin_And2018_Cyb	THE-B12	1	BD29017_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B12	Cytb	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	ACCCCTCCTCATATCCAACCA	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29017_Mgig_Bot2012_COI	THE-B12	1	BD29017_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	TCTTATTCGTTGGAGACTTTATAACCCT	133.63559
BD29017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15206	WGS84	BD29017	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29017_Mjap_Gib2022_COI	THE-B12	1	BD29017_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29017	Sample	BD29017	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B12	COI	45	BD29017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B12	AATTTAATTGTGACTAGGCATGGTCTG	133.63559
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29018_Cmae_Bot2011_COI	THE-B11	1	BD29018_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	ATGAACAGTCTATCCTCCTTTAG	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29018_Hsan_Wil2021_COI	THE-B11	1	BD29018_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	CCTGGGCCGGTATAGTAGGT	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29018_Mros_Gib2022_COI	THE-B11	1	BD29018_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29018_Msal_Bot2012_COI	THE-B11	1	BD29018_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	GYTAGTTCCRATGATGTTAGCTG	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29018_Mstr_Wil2021_COI	THE-B11	1	BD29018_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	GTAGTGGTAACTACTCATGCTTTA	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29018_Pvir_Dia2013_COI	THE-B11	1	BD29018_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	CTTAGTGGCATTAATTCGDAATCC	133.6357
BD29018	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.151	WGS84	BD29018	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29018_Rhar_Sim2018_COI	THE-B11	1	BD29018_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29018	Sample	BD29018	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B11	COI	45	BD29018	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B11	CTGCTGCTATTGCTCATG	133.6357
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15116	WGS84	BD29020	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29020_Asen_Wil2025_COI	THE-B09	1	BD29020_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	GTTGTTGTTACAACACATGCTCTTTTG	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29020_Cjap_Sim2018_COI	THE-B09	1	BD29020_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29020_Esin_And2018_Cyb	THE-B09	1	BD29020_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B09	Cytb	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	ACCCCTCCTCATATCCAACCA	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	35.177	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29020_Mgig_Bot2012_COI	THE-B09	1	BD29020_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	TCTTATTCGTTGGAGACTTTATAACCCT	133.63801
BD29020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15116	WGS84	BD29020	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29020_Mjap_Gib2022_COI	THE-B09	1	BD29020_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29020	Sample	BD29020	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B09	COI	45	BD29020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B09	AATTTAATTGTGACTAGGCATGGTCTG	133.63801
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29021_Cmae_Bot2011_COI	THE-B08	1	BD29021_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	ATGAACAGTCTATCCTCCTTTAG	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29021_Hsan_Wil2021_COI	THE-B08	1	BD29021_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	CCTGGGCCGGTATAGTAGGT	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29021_Mros_Gib2022_COI	THE-B08	1	BD29021_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29021_Msal_Bot2012_COI	THE-B08	1	BD29021_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	GYTAGTTCCRATGATGTTAGCTG	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29021_Mstr_Wil2021_COI	THE-B08	1	BD29021_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	GTAGTGGTAACTACTCATGCTTTA	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29021_Pvir_Dia2013_COI	THE-B08	1	BD29021_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	CTTAGTGGCATTAATTCGDAATCC	133.63934
BD29021	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15129	WGS84	BD29021	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29021_Rhar_Sim2018_COI	THE-B08	1	BD29021_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29021	Sample	BD29021	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B08	COI	45	BD29021	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B08	CTGCTGCTATTGCTCATG	133.63934
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29023_Aamu_Bax2006_COI	THE-B06	1	BD29023_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	GCACAACCGGGATCTTTACTTC	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29023_Pcan_Bot2011_IGS	THE-B06	1	BD29023_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B06	IGS	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	CGTAATCCTCAGTACTGGCTA	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29023_Pper_Dia2013_COI	THE-B06	1	BD29023_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	CTTAGTGGCATTAATTCGDAATCC	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29023_Sspa_Oph2007_COI	THE-B06	1	BD29023_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	GAAGGCCTTCCGATGATC	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15118	WGS84	BD29023	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29023_Upin_Bot2011_COI	THE-B06	1	BD29023_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	CTTTAATTACAGCGTTTTTATTGTTGT	133.64194
BD29023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15118	WGS84	BD29023	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29023_Vgib_Wil2025_COI	THE-B06	1	BD29023_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29023	Sample	BD29023	targeted	Absent	Sample	Vgib_Wil2025_COI	THE-B06	COI	45	BD29023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B06	CCCCTCTTTACAAGTTCTTTAGGGT	133.64194
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-32.15019	WGS84	BD29024	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29024_Asen_Wil2025_COI	THE-B05	1	BD29024_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Asen_Wil2025_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	GTTGTTGTTACAACACATGCTCTTTTG	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29024_Cjap_Sim2018_COI	THE-B05	1	BD29024_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29024_Esin_And2018_Cyb	THE-B05	1	BD29024_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B05	Cytb	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	ACCCCTCCTCATATCCAACCA	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	34.027	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29024_Mgig_Bot2012_COI	THE-B05	1	BD29024_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Present	Sample	Mgig_Bot2012_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	TCTTATTCGTTGGAGACTTTATAACCCT	133.64111
BD29024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15019	WGS84	BD29024	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29024_Mjap_Gib2022_COI	THE-B05	1	BD29024_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29024	Sample	BD29024	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B05	COI	45	BD29024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B05	AATTTAATTGTGACTAGGCATGGTCTG	133.64111
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29025_Cmae_Bot2011_COI	THE-B04	1	BD29025_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	ATGAACAGTCTATCCTCCTTTAG	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29025_Hsan_Wil2021_COI	THE-B04	1	BD29025_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	CCTGGGCCGGTATAGTAGGT	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29025_Mros_Gib2022_COI	THE-B04	1	BD29025_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Mros_Gib2022_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29025_Msal_Bot2012_COI	THE-B04	1	BD29025_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Msal_Bot2012_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	GYTAGTTCCRATGATGTTAGCTG	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29025_Mstr_Wil2021_COI	THE-B04	1	BD29025_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	GTAGTGGTAACTACTCATGCTTTA	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29025_Pvir_Dia2013_COI	THE-B04	1	BD29025_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	CTTAGTGGCATTAATTCGDAATCC	133.63966
BD29025	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15014	WGS84	BD29025	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29025_Rhar_Sim2018_COI	THE-B04	1	BD29025_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29025	Sample	BD29025	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-B04	COI	45	BD29025	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B04	CTGCTGCTATTGCTCATG	133.63966
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29027_Aamu_Bax2006_COI	THE-B02	1	BD29027_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	GCACAACCGGGATCTTTACTTC	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29027_Pcan_Bot2011_IGS	THE-B02	1	BD29027_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-B02	IGS	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	CGTAATCCTCAGTACTGGCTA	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29027_Pper_Dia2013_COI	THE-B02	1	BD29027_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Pper_Dia2013_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	CTTAGTGGCATTAATTCGDAATCC	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29027_Sspa_Oph2007_COI	THE-B02	1	BD29027_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	GAAGGCCTTCCGATGATC	133.63706
BD29027	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.15015	WGS84	BD29027	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29027_Upin_Bot2011_COI	THE-B02	1	BD29027_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29027	Sample	BD29027	targeted	Absent	Sample	Upin_Bot2011_COI	THE-B02	COI	45	BD29027	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B02	CTTTAATTACAGCGTTTTTATTGTTGT	133.63706
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29028_Cjap_Sim2018_COI	THE-B01	1	BD29028_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29028_Esin_And2018_Cyb	THE-B01	1	BD29028_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Esin_And2018_Cyb	THE-B01	Cytb	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	ACCCCTCCTCATATCCAACCA	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29028_Mgig_Bot2012_COI	THE-B01	1	BD29028_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	TCTTATTCGTTGGAGACTTTATAACCCT	133.63559
BD29028	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-32.14995	WGS84	BD29028	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Summer	23/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29028_Mjap_Gib2022_COI	THE-B01	1	BD29028_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29028	Sample	BD29028	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-B01	COI	45	BD29028	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-B01	AATTTAATTGTGACTAGGCATGGTCTG	133.63559
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29029_Cmae_Bot2011_COI	PGI-B35	1	BD29029_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	ATGAACAGTCTATCCTCCTTTAG	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29029_Hsan_Wil2021_COI	PGI-B35	1	BD29029_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	CCTGGGCCGGTATAGTAGGT	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29029_Mros_Gib2022_COI	PGI-B35	1	BD29029_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29029_Msal_Bot2012_COI	PGI-B35	1	BD29029_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	GYTAGTTCCRATGATGTTAGCTG	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29029_Mstr_Wil2021_COI	PGI-B35	1	BD29029_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	GTAGTGGTAACTACTCATGCTTTA	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29029_Pvir_Dia2013_COI	PGI-B35	1	BD29029_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	CTTAGTGGCATTAATTCGDAATCC	137.7706
BD29029	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01775	WGS84	BD29029	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29029_Rhar_Sim2018_COI	PGI-B35	1	BD29029_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29029	Sample	BD29029	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B35	COI	45	BD29029	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B35	CTGCTGCTATTGCTCATG	137.7706
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29030_Aamu_Bax2006_COI	PGI-B34	1	BD29030_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	GCACAACCGGGATCTTTACTTC	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29030_Pcan_Bot2011_IGS	PGI-B34	1	BD29030_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B34	IGS	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	CGTAATCCTCAGTACTGGCTA	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29030_Pper_Dia2013_COI	PGI-B34	1	BD29030_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	CTTAGTGGCATTAATTCGDAATCC	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29030_Sspa_Oph2007_COI	PGI-B34	1	BD29030_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	GAAGGCCTTCCGATGATC	137.76884
BD29030	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01782	WGS84	BD29030	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29030_Upin_Bot2011_COI	PGI-B34	1	BD29030_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29030	Sample	BD29030	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B34	COI	45	BD29030	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B34	CTTTAATTACAGCGTTTTTATTGTTGT	137.76884
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29031_Cjap_Sim2018_COI	PGI-B33	1	BD29031_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29031_Esin_And2018_Cyb	PGI-B33	1	BD29031_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B33	Cytb	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	ACCCCTCCTCATATCCAACCA	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29031_Mgig_Bot2012_COI	PGI-B33	1	BD29031_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	TCTTATTCGTTGGAGACTTTATAACCCT	137.76708
BD29031	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01777	WGS84	BD29031	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29031_Mjap_Gib2022_COI	PGI-B33	1	BD29031_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29031	Sample	BD29031	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B33	COI	45	BD29031	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B33	AATTTAATTGTGACTAGGCATGGTCTG	137.76708
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29032_Cmae_Bot2011_COI	PGI-B32	1	BD29032_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	ATGAACAGTCTATCCTCCTTTAG	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29032_Hsan_Wil2021_COI	PGI-B32	1	BD29032_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	CCTGGGCCGGTATAGTAGGT	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29032_Mros_Gib2022_COI	PGI-B32	1	BD29032_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29032_Msal_Bot2012_COI	PGI-B32	1	BD29032_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	GYTAGTTCCRATGATGTTAGCTG	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29032_Mstr_Wil2021_COI	PGI-B32	1	BD29032_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	GTAGTGGTAACTACTCATGCTTTA	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29032_Pvir_Dia2013_COI	PGI-B32	1	BD29032_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	CTTAGTGGCATTAATTCGDAATCC	137.76542
BD29032	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01781	WGS84	BD29032	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29032_Rhar_Sim2018_COI	PGI-B32	1	BD29032_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29032	Sample	BD29032	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B32	COI	45	BD29032	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B32	CTGCTGCTATTGCTCATG	137.76542
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29034_Aamu_Bax2006_COI	PGI-B30	1	BD29034_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	GCACAACCGGGATCTTTACTTC	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29034_Pcan_Bot2011_IGS	PGI-B30	1	BD29034_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B30	IGS	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	CGTAATCCTCAGTACTGGCTA	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29034_Pper_Dia2013_COI	PGI-B30	1	BD29034_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	CTTAGTGGCATTAATTCGDAATCC	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29034_Sspa_Oph2007_COI	PGI-B30	1	BD29034_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	GAAGGCCTTCCGATGATC	137.77147
BD29034	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01892	WGS84	BD29034	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29034_Upin_Bot2011_COI	PGI-B30	1	BD29034_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29034	Sample	BD29034	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B30	COI	45	BD29034	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B30	CTTTAATTACAGCGTTTTTATTGTTGT	137.77147
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29035_Cjap_Sim2018_COI	PGI-B29	1	BD29035_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29035_Esin_And2018_Cyb	PGI-B29	1	BD29035_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B29	Cytb	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	ACCCCTCCTCATATCCAACCA	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29035_Mgig_Bot2012_COI	PGI-B29	1	BD29035_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	TCTTATTCGTTGGAGACTTTATAACCCT	137.76983
BD29035	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01897	WGS84	BD29035	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29035_Mjap_Gib2022_COI	PGI-B29	1	BD29035_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29035	Sample	BD29035	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B29	COI	45	BD29035	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B29	AATTTAATTGTGACTAGGCATGGTCTG	137.76983
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29036_Cmae_Bot2011_COI	PGI-B28	1	BD29036_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	ATGAACAGTCTATCCTCCTTTAG	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29036_Hsan_Wil2021_COI	PGI-B28	1	BD29036_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	CCTGGGCCGGTATAGTAGGT	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29036_Mros_Gib2022_COI	PGI-B28	1	BD29036_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29036_Msal_Bot2012_COI	PGI-B28	1	BD29036_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	GYTAGTTCCRATGATGTTAGCTG	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29036_Mstr_Wil2021_COI	PGI-B28	1	BD29036_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	GTAGTGGTAACTACTCATGCTTTA	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29036_Pvir_Dia2013_COI	PGI-B28	1	BD29036_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	CTTAGTGGCATTAATTCGDAATCC	137.76811
BD29036	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01895	WGS84	BD29036	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29036_Rhar_Sim2018_COI	PGI-B28	1	BD29036_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29036	Sample	BD29036	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B28	COI	45	BD29036	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B28	CTGCTGCTATTGCTCATG	137.76811
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29038_Aamu_Bax2006_COI	PGI-B26	1	BD29038_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	GCACAACCGGGATCTTTACTTC	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29038_Pcan_Bot2011_IGS	PGI-B26	1	BD29038_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B26	IGS	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	CGTAATCCTCAGTACTGGCTA	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29038_Pper_Dia2013_COI	PGI-B26	1	BD29038_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	CTTAGTGGCATTAATTCGDAATCC	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29038_Sspa_Oph2007_COI	PGI-B26	1	BD29038_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	GAAGGCCTTCCGATGATC	137.7646
BD29038	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.01901	WGS84	BD29038	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29038_Upin_Bot2011_COI	PGI-B26	1	BD29038_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29038	Sample	BD29038	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B26	COI	45	BD29038	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B26	CTTTAATTACAGCGTTTTTATTGTTGT	137.7646
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29039_Cjap_Sim2018_COI	PGI-B25	1	BD29039_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29039_Esin_And2018_Cyb	PGI-B25	1	BD29039_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B25	Cytb	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	ACCCCTCCTCATATCCAACCA	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29039_Mgig_Bot2012_COI	PGI-B25	1	BD29039_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	TCTTATTCGTTGGAGACTTTATAACCCT	137.77239
BD29039	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02011	WGS84	BD29039	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29039_Mjap_Gib2022_COI	PGI-B25	1	BD29039_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29039	Sample	BD29039	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B25	COI	45	BD29039	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B25	AATTTAATTGTGACTAGGCATGGTCTG	137.77239
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29041_Aamu_Bax2006_COI	PGI-B23	1	BD29041_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	GCACAACCGGGATCTTTACTTC	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29041_Pcan_Bot2011_IGS	PGI-B23	1	BD29041_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B23	IGS	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	CGTAATCCTCAGTACTGGCTA	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29041_Pper_Dia2013_COI	PGI-B23	1	BD29041_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	CTTAGTGGCATTAATTCGDAATCC	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29041_Sspa_Oph2007_COI	PGI-B23	1	BD29041_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	GAAGGCCTTCCGATGATC	137.76897
BD29041	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02027	WGS84	BD29041	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29041_Upin_Bot2011_COI	PGI-B23	1	BD29041_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29041	Sample	BD29041	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B23	COI	45	BD29041	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B23	CTTTAATTACAGCGTTTTTATTGTTGT	137.76897
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29042_Cjap_Sim2018_COI	PGI-B22	1	BD29042_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29042_Esin_And2018_Cyb	PGI-B22	1	BD29042_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B22	Cytb	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	ACCCCTCCTCATATCCAACCA	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29042_Mgig_Bot2012_COI	PGI-B22	1	BD29042_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	TCTTATTCGTTGGAGACTTTATAACCCT	137.76718
BD29042	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29042	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29042_Mjap_Gib2022_COI	PGI-B22	1	BD29042_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29042	Sample	BD29042	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B22	COI	45	BD29042	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B22	AATTTAATTGTGACTAGGCATGGTCTG	137.76718
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29043_Cmae_Bot2011_COI	PGI-B21	1	BD29043_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	ATGAACAGTCTATCCTCCTTTAG	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29043_Hsan_Wil2021_COI	PGI-B21	1	BD29043_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	CCTGGGCCGGTATAGTAGGT	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29043_Mros_Gib2022_COI	PGI-B21	1	BD29043_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29043_Msal_Bot2012_COI	PGI-B21	1	BD29043_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	GYTAGTTCCRATGATGTTAGCTG	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29043_Mstr_Wil2021_COI	PGI-B21	1	BD29043_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	GTAGTGGTAACTACTCATGCTTTA	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29043_Pvir_Dia2013_COI	PGI-B21	1	BD29043_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	CTTAGTGGCATTAATTCGDAATCC	137.76543
BD29043	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02029	WGS84	BD29043	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29043_Rhar_Sim2018_COI	PGI-B21	1	BD29043_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29043	Sample	BD29043	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B21	COI	45	BD29043	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B21	CTGCTGCTATTGCTCATG	137.76543
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29045_Aamu_Bax2006_COI	PGI-B19	1	BD29045_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	GCACAACCGGGATCTTTACTTC	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29045_Pcan_Bot2011_IGS	PGI-B19	1	BD29045_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B19	IGS	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	CGTAATCCTCAGTACTGGCTA	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29045_Pper_Dia2013_COI	PGI-B19	1	BD29045_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	CTTAGTGGCATTAATTCGDAATCC	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29045_Sspa_Oph2007_COI	PGI-B19	1	BD29045_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	GAAGGCCTTCCGATGATC	137.77334
BD29045	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02128	WGS84	BD29045	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29045_Upin_Bot2011_COI	PGI-B19	1	BD29045_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29045	Sample	BD29045	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B19	COI	45	BD29045	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B19	CTTTAATTACAGCGTTTTTATTGTTGT	137.77334
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29046_Cjap_Sim2018_COI	PGI-B18	1	BD29046_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29046_Esin_And2018_Cyb	PGI-B18	1	BD29046_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B18	Cytb	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	ACCCCTCCTCATATCCAACCA	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29046_Mgig_Bot2012_COI	PGI-B18	1	BD29046_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	TCTTATTCGTTGGAGACTTTATAACCCT	137.77165
BD29046	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.0213	WGS84	BD29046	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29046_Mjap_Gib2022_COI	PGI-B18	1	BD29046_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29046	Sample	BD29046	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B18	COI	45	BD29046	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B18	AATTTAATTGTGACTAGGCATGGTCTG	137.77165
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29047_Cmae_Bot2011_COI	PGI-B17	1	BD29047_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	ATGAACAGTCTATCCTCCTTTAG	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29047_Hsan_Wil2021_COI	PGI-B17	1	BD29047_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	CCTGGGCCGGTATAGTAGGT	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29047_Mros_Gib2022_COI	PGI-B17	1	BD29047_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29047_Msal_Bot2012_COI	PGI-B17	1	BD29047_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	GYTAGTTCCRATGATGTTAGCTG	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29047_Mstr_Wil2021_COI	PGI-B17	1	BD29047_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	GTAGTGGTAACTACTCATGCTTTA	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29047_Pvir_Dia2013_COI	PGI-B17	1	BD29047_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	CTTAGTGGCATTAATTCGDAATCC	137.76988
BD29047	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02141	WGS84	BD29047	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29047_Rhar_Sim2018_COI	PGI-B17	1	BD29047_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29047	Sample	BD29047	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B17	COI	45	BD29047	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B17	CTGCTGCTATTGCTCATG	137.76988
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29049_Aamu_Bax2006_COI	PGI-B15	1	BD29049_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	GCACAACCGGGATCTTTACTTC	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29049_Pcan_Bot2011_IGS	PGI-B15	1	BD29049_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B15	IGS	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	CGTAATCCTCAGTACTGGCTA	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29049_Pper_Dia2013_COI	PGI-B15	1	BD29049_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	CTTAGTGGCATTAATTCGDAATCC	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29049_Sspa_Oph2007_COI	PGI-B15	1	BD29049_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	GAAGGCCTTCCGATGATC	137.77077
BD29049	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02261	WGS84	BD29049	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29049_Upin_Bot2011_COI	PGI-B15	1	BD29049_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29049	Sample	BD29049	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B15	COI	45	BD29049	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B15	CTTTAATTACAGCGTTTTTATTGTTGT	137.77077
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29050_Cmae_Bot2011_COI	PGI-B14	1	BD29050_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	ATGAACAGTCTATCCTCCTTTAG	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29050_Hsan_Wil2021_COI	PGI-B14	1	BD29050_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	CCTGGGCCGGTATAGTAGGT	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29050_Mros_Gib2022_COI	PGI-B14	1	BD29050_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29050_Msal_Bot2012_COI	PGI-B14	1	BD29050_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	GYTAGTTCCRATGATGTTAGCTG	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29050_Mstr_Wil2021_COI	PGI-B14	1	BD29050_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	GTAGTGGTAACTACTCATGCTTTA	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29050_Pvir_Dia2013_COI	PGI-B14	1	BD29050_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	CTTAGTGGCATTAATTCGDAATCC	137.76906
BD29050	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02265	WGS84	BD29050	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29050_Rhar_Sim2018_COI	PGI-B14	1	BD29050_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29050	Sample	BD29050	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B14	COI	45	BD29050	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B14	CTGCTGCTATTGCTCATG	137.76906
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29052_Aamu_Bax2006_COI	PGI-B12	1	BD29052_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	GCACAACCGGGATCTTTACTTC	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29052_Pcan_Bot2011_IGS	PGI-B12	1	BD29052_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B12	IGS	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	CGTAATCCTCAGTACTGGCTA	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29052_Pper_Dia2013_COI	PGI-B12	1	BD29052_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	CTTAGTGGCATTAATTCGDAATCC	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29052_Sspa_Oph2007_COI	PGI-B12	1	BD29052_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	GAAGGCCTTCCGATGATC	137.76561
BD29052	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02284	WGS84	BD29052	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29052_Upin_Bot2011_COI	PGI-B12	1	BD29052_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29052	Sample	BD29052	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B12	COI	45	BD29052	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B12	CTTTAATTACAGCGTTTTTATTGTTGT	137.76561
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29053_Cjap_Sim2018_COI	PGI-B11	1	BD29053_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29053_Esin_And2018_Cyb	PGI-B11	1	BD29053_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B11	Cytb	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	ACCCCTCCTCATATCCAACCA	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29053_Mgig_Bot2012_COI	PGI-B11	1	BD29053_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	TCTTATTCGTTGGAGACTTTATAACCCT	137.76396
BD29053	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02295	WGS84	BD29053	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29053_Mjap_Gib2022_COI	PGI-B11	1	BD29053_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29053	Sample	BD29053	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B11	COI	45	BD29053	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B11	AATTTAATTGTGACTAGGCATGGTCTG	137.76396
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29054_Cmae_Bot2011_COI	PGI-B10	1	BD29054_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	ATGAACAGTCTATCCTCCTTTAG	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29054_Hsan_Wil2021_COI	PGI-B10	1	BD29054_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	CCTGGGCCGGTATAGTAGGT	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29054_Mros_Gib2022_COI	PGI-B10	1	BD29054_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29054_Msal_Bot2012_COI	PGI-B10	1	BD29054_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	GYTAGTTCCRATGATGTTAGCTG	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29054_Mstr_Wil2021_COI	PGI-B10	1	BD29054_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	GTAGTGGTAACTACTCATGCTTTA	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29054_Pvir_Dia2013_COI	PGI-B10	1	BD29054_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	CTTAGTGGCATTAATTCGDAATCC	137.77167
BD29054	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02393	WGS84	BD29054	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29054_Rhar_Sim2018_COI	PGI-B10	1	BD29054_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29054	Sample	BD29054	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B10	COI	45	BD29054	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B10	CTGCTGCTATTGCTCATG	137.77167
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29056_Aamu_Bax2006_COI	PGI-B08	1	BD29056_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	GCACAACCGGGATCTTTACTTC	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29056_Pcan_Bot2011_IGS	PGI-B08	1	BD29056_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B08	IGS	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	CGTAATCCTCAGTACTGGCTA	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29056_Pper_Dia2013_COI	PGI-B08	1	BD29056_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	CTTAGTGGCATTAATTCGDAATCC	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29056_Sspa_Oph2007_COI	PGI-B08	1	BD29056_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	GAAGGCCTTCCGATGATC	137.76814
BD29056	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02394	WGS84	BD29056	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29056_Upin_Bot2011_COI	PGI-B08	1	BD29056_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29056	Sample	BD29056	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B08	COI	45	BD29056	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B08	CTTTAATTACAGCGTTTTTATTGTTGT	137.76814
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29057_Cjap_Sim2018_COI	PGI-B07	1	BD29057_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29057_Esin_And2018_Cyb	PGI-B07	1	BD29057_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B07	Cytb	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	ACCCCTCCTCATATCCAACCA	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29057_Mgig_Bot2012_COI	PGI-B07	1	BD29057_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	TCTTATTCGTTGGAGACTTTATAACCCT	137.76643
BD29057	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02409	WGS84	BD29057	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29057_Mjap_Gib2022_COI	PGI-B07	1	BD29057_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29057	Sample	BD29057	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B07	COI	45	BD29057	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B07	AATTTAATTGTGACTAGGCATGGTCTG	137.76643
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29058_Cmae_Bot2011_COI	PGI-B06	1	BD29058_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	ATGAACAGTCTATCCTCCTTTAG	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29058_Hsan_Wil2021_COI	PGI-B06	1	BD29058_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	CCTGGGCCGGTATAGTAGGT	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29058_Mros_Gib2022_COI	PGI-B06	1	BD29058_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29058_Msal_Bot2012_COI	PGI-B06	1	BD29058_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	GYTAGTTCCRATGATGTTAGCTG	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29058_Mstr_Wil2021_COI	PGI-B06	1	BD29058_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	GTAGTGGTAACTACTCATGCTTTA	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29058_Pvir_Dia2013_COI	PGI-B06	1	BD29058_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	CTTAGTGGCATTAATTCGDAATCC	137.76476
BD29058	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02407	WGS84	BD29058	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29058_Rhar_Sim2018_COI	PGI-B06	1	BD29058_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29058	Sample	BD29058	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B06	COI	45	BD29058	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B06	CTGCTGCTATTGCTCATG	137.76476
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29060_Cjap_Sim2018_COI	PGI-B04	1	BD29060_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29060_Esin_And2018_Cyb	PGI-B04	1	BD29060_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-B04	Cytb	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	ACCCCTCCTCATATCCAACCA	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29060_Mgig_Bot2012_COI	PGI-B04	1	BD29060_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	TCTTATTCGTTGGAGACTTTATAACCCT	137.76916
BD29060	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02521	WGS84	BD29060	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29060_Mjap_Gib2022_COI	PGI-B04	1	BD29060_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29060	Sample	BD29060	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-B04	COI	45	BD29060	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B04	AATTTAATTGTGACTAGGCATGGTCTG	137.76916
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29061_Cmae_Bot2011_COI	PGI-B03	1	BD29061_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	ATGAACAGTCTATCCTCCTTTAG	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29061_Hsan_Wil2021_COI	PGI-B03	1	BD29061_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	CCTGGGCCGGTATAGTAGGT	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29061_Mros_Gib2022_COI	PGI-B03	1	BD29061_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29061_Msal_Bot2012_COI	PGI-B03	1	BD29061_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	GYTAGTTCCRATGATGTTAGCTG	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29061_Mstr_Wil2021_COI	PGI-B03	1	BD29061_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	GTAGTGGTAACTACTCATGCTTTA	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29061_Pvir_Dia2013_COI	PGI-B03	1	BD29061_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	CTTAGTGGCATTAATTCGDAATCC	137.76729
BD29061	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02534	WGS84	BD29061	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29061_Rhar_Sim2018_COI	PGI-B03	1	BD29061_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29061	Sample	BD29061	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-B03	COI	45	BD29061	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B03	CTGCTGCTATTGCTCATG	137.76729
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29063_Aamu_Bax2006_COI	PGI-B01	1	BD29063_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	GCACAACCGGGATCTTTACTTC	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29063_Pcan_Bot2011_IGS	PGI-B01	1	BD29063_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-B01	IGS	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	CGTAATCCTCAGTACTGGCTA	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29063_Pper_Dia2013_COI	PGI-B01	1	BD29063_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	CTTAGTGGCATTAATTCGDAATCC	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29063_Sspa_Oph2007_COI	PGI-B01	1	BD29063_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	GAAGGCCTTCCGATGATC	137.76396
BD29063	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14703	-35.02543	WGS84	BD29063	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29063_Upin_Bot2011_COI	PGI-B01	1	BD29063_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29063	Sample	BD29063	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-B01	COI	45	BD29063	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-B01	CTTTAATTACAGCGTTTTTATTGTTGT	137.76396
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29064_Cjap_Sim2018_COI	KPT-B35	1	BD29064_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29064_Esin_And2018_Cyb	KPT-B35	1	BD29064_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B35	Cytb	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	ACCCCTCCTCATATCCAACCA	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29064_Mgig_Bot2012_COI	KPT-B35	1	BD29064_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	TCTTATTCGTTGGAGACTTTATAACCCT	137.78064
BD29064	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96128	WGS84	BD29064	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29064_Mjap_Gib2022_COI	KPT-B35	1	BD29064_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29064	Sample	BD29064	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B35	COI	45	BD29064	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B35	AATTTAATTGTGACTAGGCATGGTCTG	137.78064
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29065_Cmae_Bot2011_COI	KPT-B34	1	BD29065_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	ATGAACAGTCTATCCTCCTTTAG	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29065_Hsan_Wil2021_COI	KPT-B34	1	BD29065_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	CCTGGGCCGGTATAGTAGGT	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29065_Mros_Gib2022_COI	KPT-B34	1	BD29065_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29065_Msal_Bot2012_COI	KPT-B34	1	BD29065_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	GYTAGTTCCRATGATGTTAGCTG	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29065_Mstr_Wil2021_COI	KPT-B34	1	BD29065_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	GTAGTGGTAACTACTCATGCTTTA	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29065_Pvir_Dia2013_COI	KPT-B34	1	BD29065_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	CTTAGTGGCATTAATTCGDAATCC	137.77922
BD29065	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96121	WGS84	BD29065	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29065_Rhar_Sim2018_COI	KPT-B34	1	BD29065_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29065	Sample	BD29065	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B34	COI	45	BD29065	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B34	CTGCTGCTATTGCTCATG	137.77922
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29067_Aamu_Bax2006_COI	KPT-B32	1	BD29067_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	GCACAACCGGGATCTTTACTTC	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29067_Pcan_Bot2011_IGS	KPT-B32	1	BD29067_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B32	IGS	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	CGTAATCCTCAGTACTGGCTA	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29067_Pper_Dia2013_COI	KPT-B32	1	BD29067_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	CTTAGTGGCATTAATTCGDAATCC	137.77676
BD29067	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96132	WGS84	BD29067	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29067_Upin_Bot2011_COI	KPT-B32	1	BD29067_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29067	Sample	BD29067	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B32	COI	45	BD29067	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B32	CTTTAATTACAGCGTTTTTATTGTTGT	137.77676
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29068_Cjap_Sim2018_COI	KPT-B31	1	BD29068_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29068_Esin_And2018_Cyb	KPT-B31	1	BD29068_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B31	Cytb	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	ACCCCTCCTCATATCCAACCA	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29068_Mgig_Bot2012_COI	KPT-B31	1	BD29068_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	TCTTATTCGTTGGAGACTTTATAACCCT	137.77987
BD29068	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96021	WGS84	BD29068	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29068_Mjap_Gib2022_COI	KPT-B31	1	BD29068_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29068	Sample	BD29068	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B31	COI	45	BD29068	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B31	AATTTAATTGTGACTAGGCATGGTCTG	137.77987
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29069_Cmae_Bot2011_COI	KPT-B30	1	BD29069_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	ATGAACAGTCTATCCTCCTTTAG	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29069_Hsan_Wil2021_COI	KPT-B30	1	BD29069_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	CCTGGGCCGGTATAGTAGGT	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29069_Mros_Gib2022_COI	KPT-B30	1	BD29069_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29069_Msal_Bot2012_COI	KPT-B30	1	BD29069_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	GYTAGTTCCRATGATGTTAGCTG	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29069_Mstr_Wil2021_COI	KPT-B30	1	BD29069_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	GTAGTGGTAACTACTCATGCTTTA	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29069_Pvir_Dia2013_COI	KPT-B30	1	BD29069_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	CTTAGTGGCATTAATTCGDAATCC	137.77857
BD29069	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96046	WGS84	BD29069	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29069_Rhar_Sim2018_COI	KPT-B30	1	BD29069_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29069	Sample	BD29069	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B30	COI	45	BD29069	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B30	CTGCTGCTATTGCTCATG	137.77857
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29070_Aamu_Bax2006_COI	KPT-B29	1	BD29070_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	GCACAACCGGGATCTTTACTTC	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29070_Pcan_Bot2011_IGS	KPT-B29	1	BD29070_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B29	IGS	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	CGTAATCCTCAGTACTGGCTA	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29070_Pper_Dia2013_COI	KPT-B29	1	BD29070_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	CTTAGTGGCATTAATTCGDAATCC	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29070_Sspa_Oph2007_COI	KPT-B29	1	BD29070_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	GAAGGCCTTCCGATGATC	137.7774
BD29070	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96043	WGS84	BD29070	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29070_Upin_Bot2011_COI	KPT-B29	1	BD29070_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29070	Sample	BD29070	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B29	COI	45	BD29070	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B29	CTTTAATTACAGCGTTTTTATTGTTGT	137.7774
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29071_Cjap_Sim2018_COI	KPT-B28	1	BD29071_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29071_Esin_And2018_Cyb	KPT-B28	1	BD29071_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B28	Cytb	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	ACCCCTCCTCATATCCAACCA	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29071_Mgig_Bot2012_COI	KPT-B28	1	BD29071_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	TCTTATTCGTTGGAGACTTTATAACCCT	137.77599
BD29071	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96051	WGS84	BD29071	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29071_Mjap_Gib2022_COI	KPT-B28	1	BD29071_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29071	Sample	BD29071	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B28	COI	45	BD29071	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B28	AATTTAATTGTGACTAGGCATGGTCTG	137.77599
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29072_Cmae_Bot2011_COI	KPT-B27	1	BD29072_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	ATGAACAGTCTATCCTCCTTTAG	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29072_Hsan_Wil2021_COI	KPT-B27	1	BD29072_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	CCTGGGCCGGTATAGTAGGT	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29072_Mros_Gib2022_COI	KPT-B27	1	BD29072_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29072_Msal_Bot2012_COI	KPT-B27	1	BD29072_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	GYTAGTTCCRATGATGTTAGCTG	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29072_Mstr_Wil2021_COI	KPT-B27	1	BD29072_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	GTAGTGGTAACTACTCATGCTTTA	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29072_Pvir_Dia2013_COI	KPT-B27	1	BD29072_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	CTTAGTGGCATTAATTCGDAATCC	137.77929
BD29072	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95943	WGS84	BD29072	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29072_Rhar_Sim2018_COI	KPT-B27	1	BD29072_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29072	Sample	BD29072	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B27	COI	45	BD29072	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B27	CTGCTGCTATTGCTCATG	137.77929
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29074_Aamu_Bax2006_COI	KPT-B25	1	BD29074_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	GCACAACCGGGATCTTTACTTC	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29074_Pcan_Bot2011_IGS	KPT-B25	1	BD29074_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B25	IGS	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	CGTAATCCTCAGTACTGGCTA	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29074_Pper_Dia2013_COI	KPT-B25	1	BD29074_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	CTTAGTGGCATTAATTCGDAATCC	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29074_Sspa_Oph2007_COI	KPT-B25	1	BD29074_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	GAAGGCCTTCCGATGATC	137.77663
BD29074	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95953	WGS84	BD29074	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29074_Upin_Bot2011_COI	KPT-B25	1	BD29074_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29074	Sample	BD29074	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B25	COI	45	BD29074	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B25	CTTTAATTACAGCGTTTTTATTGTTGT	137.77663
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29075_Cjap_Sim2018_COI	KPT-B24	1	BD29075_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29075_Esin_And2018_Cyb	KPT-B24	1	BD29075_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B24	Cytb	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	ACCCCTCCTCATATCCAACCA	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29075_Mgig_Bot2012_COI	KPT-B24	1	BD29075_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	TCTTATTCGTTGGAGACTTTATAACCCT	137.77851
BD29075	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95858	WGS84	BD29075	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29075_Mjap_Gib2022_COI	KPT-B24	1	BD29075_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29075	Sample	BD29075	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B24	COI	45	BD29075	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B24	AATTTAATTGTGACTAGGCATGGTCTG	137.77851
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29076_Cmae_Bot2011_COI	KPT-B23	1	BD29076_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	ATGAACAGTCTATCCTCCTTTAG	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29076_Hsan_Wil2021_COI	KPT-B23	1	BD29076_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	CCTGGGCCGGTATAGTAGGT	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29076_Msal_Bot2012_COI	KPT-B23	1	BD29076_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	GYTAGTTCCRATGATGTTAGCTG	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29076_Mstr_Wil2021_COI	KPT-B23	1	BD29076_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	GTAGTGGTAACTACTCATGCTTTA	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29076_Pvir_Dia2013_COI	KPT-B23	1	BD29076_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	CTTAGTGGCATTAATTCGDAATCC	137.77717
BD29076	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95861	WGS84	BD29076	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29076_Rhar_Sim2018_COI	KPT-B23	1	BD29076_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29076	Sample	BD29076	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B23	COI	45	BD29076	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B23	CTGCTGCTATTGCTCATG	137.77717
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29078_Aamu_Bax2006_COI	KPT-B21	1	BD29078_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	GCACAACCGGGATCTTTACTTC	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29078_Pcan_Bot2011_IGS	KPT-B21	1	BD29078_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B21	IGS	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	CGTAATCCTCAGTACTGGCTA	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29078_Pper_Dia2013_COI	KPT-B21	1	BD29078_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	CTTAGTGGCATTAATTCGDAATCC	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29078_Sspa_Oph2007_COI	KPT-B21	1	BD29078_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	GAAGGCCTTCCGATGATC	137.77675
BD29078	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.95751	WGS84	BD29078	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29078_Upin_Bot2011_COI	KPT-B21	1	BD29078_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29078	Sample	BD29078	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B21	COI	45	BD29078	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B21	CTTTAATTACAGCGTTTTTATTGTTGT	137.77675
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29079_Cjap_Sim2018_COI	KPT-B20	1	BD29079_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29079_Esin_And2018_Cyb	KPT-B20	1	BD29079_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B20	Cytb	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	ACCCCTCCTCATATCCAACCA	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29079_Mgig_Bot2012_COI	KPT-B20	1	BD29079_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	TCTTATTCGTTGGAGACTTTATAACCCT	137.77984
BD29079	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96209	WGS84	BD29079	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29079_Mjap_Gib2022_COI	KPT-B20	1	BD29079_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29079	Sample	BD29079	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B20	COI	45	BD29079	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B20	AATTTAATTGTGACTAGGCATGGTCTG	137.77984
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29081_Aamu_Bax2006_COI	KPT-B18	1	BD29081_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	GCACAACCGGGATCTTTACTTC	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29081_Pcan_Bot2011_IGS	KPT-B18	1	BD29081_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B18	IGS	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	CGTAATCCTCAGTACTGGCTA	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29081_Pper_Dia2013_COI	KPT-B18	1	BD29081_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	CTTAGTGGCATTAATTCGDAATCC	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29081_Sspa_Oph2007_COI	KPT-B18	1	BD29081_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	GAAGGCCTTCCGATGATC	137.77734
BD29081	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29081	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29081_Upin_Bot2011_COI	KPT-B18	1	BD29081_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29081	Sample	BD29081	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B18	COI	45	BD29081	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B18	CTTTAATTACAGCGTTTTTATTGTTGT	137.77734
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29082_Cjap_Sim2018_COI	KPT-B17	1	BD29082_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29082_Esin_And2018_Cyb	KPT-B17	1	BD29082_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B17	Cytb	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	ACCCCTCCTCATATCCAACCA	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29082_Mgig_Bot2012_COI	KPT-B17	1	BD29082_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	TCTTATTCGTTGGAGACTTTATAACCCT	137.77611
BD29082	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96224	WGS84	BD29082	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29082_Mjap_Gib2022_COI	KPT-B17	1	BD29082_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29082	Sample	BD29082	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B17	COI	45	BD29082	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B17	AATTTAATTGTGACTAGGCATGGTCTG	137.77611
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29083_Cmae_Bot2011_COI	KPT-B16	1	BD29083_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	ATGAACAGTCTATCCTCCTTTAG	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29083_Hsan_Wil2021_COI	KPT-B16	1	BD29083_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	CCTGGGCCGGTATAGTAGGT	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29083_Mros_Gib2022_COI	KPT-B16	1	BD29083_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29083_Msal_Bot2012_COI	KPT-B16	1	BD29083_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	GYTAGTTCCRATGATGTTAGCTG	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29083_Mstr_Wil2021_COI	KPT-B16	1	BD29083_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	GTAGTGGTAACTACTCATGCTTTA	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29083_Pvir_Dia2013_COI	KPT-B16	1	BD29083_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	CTTAGTGGCATTAATTCGDAATCC	137.78061
BD29083	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.963	WGS84	BD29083	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29083_Rhar_Sim2018_COI	KPT-B16	1	BD29083_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29083	Sample	BD29083	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B16	COI	45	BD29083	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B16	CTGCTGCTATTGCTCATG	137.78061
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29085_Aamu_Bax2006_COI	KPT-B14	1	BD29085_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	GCACAACCGGGATCTTTACTTC	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29085_Pcan_Bot2011_IGS	KPT-B14	1	BD29085_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B14	IGS	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	CGTAATCCTCAGTACTGGCTA	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29085_Pper_Dia2013_COI	KPT-B14	1	BD29085_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	CTTAGTGGCATTAATTCGDAATCC	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29085_Sspa_Oph2007_COI	KPT-B14	1	BD29085_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	GAAGGCCTTCCGATGATC	137.77798
BD29085	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96311	WGS84	BD29085	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29085_Upin_Bot2011_COI	KPT-B14	1	BD29085_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29085	Sample	BD29085	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B14	COI	45	BD29085	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B14	CTTTAATTACAGCGTTTTTATTGTTGT	137.77798
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29086_Cjap_Sim2018_COI	KPT-B13	1	BD29086_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29086_Esin_And2018_Cyb	KPT-B13	1	BD29086_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B13	Cytb	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	ACCCCTCCTCATATCCAACCA	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29086_Mgig_Bot2012_COI	KPT-B13	1	BD29086_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	TCTTATTCGTTGGAGACTTTATAACCCT	137.77686
BD29086	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96319	WGS84	BD29086	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29086_Mjap_Gib2022_COI	KPT-B13	1	BD29086_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29086	Sample	BD29086	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B13	COI	45	BD29086	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B13	AATTTAATTGTGACTAGGCATGGTCTG	137.77686
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29087_Cmae_Bot2011_COI	KPT-B12	1	BD29087_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	ATGAACAGTCTATCCTCCTTTAG	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29087_Hsan_Wil2021_COI	KPT-B12	1	BD29087_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	CCTGGGCCGGTATAGTAGGT	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29087_Mros_Gib2022_COI	KPT-B12	1	BD29087_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29087_Msal_Bot2012_COI	KPT-B12	1	BD29087_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	GYTAGTTCCRATGATGTTAGCTG	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29087_Mstr_Wil2021_COI	KPT-B12	1	BD29087_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	GTAGTGGTAACTACTCATGCTTTA	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29087_Pvir_Dia2013_COI	KPT-B12	1	BD29087_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	CTTAGTGGCATTAATTCGDAATCC	137.7754
BD29087	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96327	WGS84	BD29087	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29087_Rhar_Sim2018_COI	KPT-B12	1	BD29087_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29087	Sample	BD29087	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B12	COI	45	BD29087	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B12	CTGCTGCTATTGCTCATG	137.7754
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29089_Aamu_Bax2006_COI	KPT-B10	1	BD29089_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	GCACAACCGGGATCTTTACTTC	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29089_Pcan_Bot2011_IGS	KPT-B10	1	BD29089_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B10	IGS	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	CGTAATCCTCAGTACTGGCTA	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29089_Pper_Dia2013_COI	KPT-B10	1	BD29089_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	CTTAGTGGCATTAATTCGDAATCC	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29089_Sspa_Oph2007_COI	KPT-B10	1	BD29089_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	GAAGGCCTTCCGATGATC	137.78008
BD29089	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96401	WGS84	BD29089	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29089_Upin_Bot2011_COI	KPT-B10	1	BD29089_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29089	Sample	BD29089	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B10	COI	45	BD29089	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B10	CTTTAATTACAGCGTTTTTATTGTTGT	137.78008
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29090_Cmae_Bot2011_COI	KPT-B09	1	BD29090_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	ATGAACAGTCTATCCTCCTTTAG	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29090_Hsan_Wil2021_COI	KPT-B09	1	BD29090_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	CCTGGGCCGGTATAGTAGGT	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29090_Mros_Gib2022_COI	KPT-B09	1	BD29090_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29090_Msal_Bot2012_COI	KPT-B09	1	BD29090_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	GYTAGTTCCRATGATGTTAGCTG	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29090_Mstr_Wil2021_COI	KPT-B09	1	BD29090_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	GTAGTGGTAACTACTCATGCTTTA	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29090_Pvir_Dia2013_COI	KPT-B09	1	BD29090_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	CTTAGTGGCATTAATTCGDAATCC	137.77871
BD29090	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96407	WGS84	BD29090	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29090_Rhar_Sim2018_COI	KPT-B09	1	BD29090_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29090	Sample	BD29090	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B09	COI	45	BD29090	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B09	CTGCTGCTATTGCTCATG	137.77871
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29092_Aamu_Bax2006_COI	KPT-B07	1	BD29092_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	GCACAACCGGGATCTTTACTTC	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29092_Pcan_Bot2011_IGS	KPT-B07	1	BD29092_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B07	IGS	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	CGTAATCCTCAGTACTGGCTA	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29092_Pper_Dia2013_COI	KPT-B07	1	BD29092_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	CTTAGTGGCATTAATTCGDAATCC	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29092_Sspa_Oph2007_COI	KPT-B07	1	BD29092_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	GAAGGCCTTCCGATGATC	137.77614
BD29092	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96418	WGS84	BD29092	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29092_Upin_Bot2011_COI	KPT-B07	1	BD29092_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29092	Sample	BD29092	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B07	COI	45	BD29092	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B07	CTTTAATTACAGCGTTTTTATTGTTGT	137.77614
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29093_Cjap_Sim2018_COI	KPT-B06	1	BD29093_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29093_Esin_And2018_Cyb	KPT-B06	1	BD29093_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B06	Cytb	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	ACCCCTCCTCATATCCAACCA	137.77479
BD29093	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96417	WGS84	BD29093	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29093_Mgig_Bot2012_COI	KPT-B06	1	BD29093_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29093	Sample	BD29093	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B06	COI	45	BD29093	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B06	TCTTATTCGTTGGAGACTTTATAACCCT	137.77479
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29094_Cmae_Bot2011_COI	KPT-B05	1	BD29094_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	ATGAACAGTCTATCCTCCTTTAG	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29094_Hsan_Wil2021_COI	KPT-B05	1	BD29094_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	CCTGGGCCGGTATAGTAGGT	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29094_Mros_Gib2022_COI	KPT-B05	1	BD29094_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29094_Msal_Bot2012_COI	KPT-B05	1	BD29094_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	GYTAGTTCCRATGATGTTAGCTG	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29094_Mstr_Wil2021_COI	KPT-B05	1	BD29094_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	GTAGTGGTAACTACTCATGCTTTA	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29094_Pvir_Dia2013_COI	KPT-B05	1	BD29094_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	CTTAGTGGCATTAATTCGDAATCC	137.77363
BD29094	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96422	WGS84	BD29094	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29094_Rhar_Sim2018_COI	KPT-B05	1	BD29094_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29094	Sample	BD29094	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B05	COI	45	BD29094	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B05	CTGCTGCTATTGCTCATG	137.77363
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29096_Aamu_Bax2006_COI	KPT-B03	1	BD29096_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	GCACAACCGGGATCTTTACTTC	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29096_Pcan_Bot2011_IGS	KPT-B03	1	BD29096_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-B03	IGS	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	CGTAATCCTCAGTACTGGCTA	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29096_Pper_Dia2013_COI	KPT-B03	1	BD29096_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	CTTAGTGGCATTAATTCGDAATCC	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29096_Sspa_Oph2007_COI	KPT-B03	1	BD29096_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	GAAGGCCTTCCGATGATC	137.77684
BD29096	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96503	WGS84	BD29096	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29096_Upin_Bot2011_COI	KPT-B03	1	BD29096_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29096	Sample	BD29096	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-B03	COI	45	BD29096	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B03	CTTTAATTACAGCGTTTTTATTGTTGT	137.77684
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29097_Cjap_Sim2018_COI	KPT-B02	1	BD29097_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29097_Esin_And2018_Cyb	KPT-B02	1	BD29097_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-B02	Cytb	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	ACCCCTCCTCATATCCAACCA	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29097_Mgig_Bot2012_COI	KPT-B02	1	BD29097_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	TCTTATTCGTTGGAGACTTTATAACCCT	137.77554
BD29097	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96509	WGS84	BD29097	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29097_Mjap_Gib2022_COI	KPT-B02	1	BD29097_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29097	Sample	BD29097	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-B02	COI	45	BD29097	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B02	AATTTAATTGTGACTAGGCATGGTCTG	137.77554
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29098_Cmae_Bot2011_COI	KPT-B01	1	BD29098_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	ATGAACAGTCTATCCTCCTTTAG	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29098_Hsan_Wil2021_COI	KPT-B01	1	BD29098_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	CCTGGGCCGGTATAGTAGGT	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29098_Mros_Gib2022_COI	KPT-B01	1	BD29098_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29098_Msal_Bot2012_COI	KPT-B01	1	BD29098_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	GYTAGTTCCRATGATGTTAGCTG	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29098_Mstr_Wil2021_COI	KPT-B01	1	BD29098_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	GTAGTGGTAACTACTCATGCTTTA	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29098_Pvir_Dia2013_COI	KPT-B01	1	BD29098_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	CTTAGTGGCATTAATTCGDAATCC	137.77442
BD29098	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.96511	WGS84	BD29098	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29098_Rhar_Sim2018_COI	KPT-B01	1	BD29098_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29098	Sample	BD29098	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-B01	COI	45	BD29098	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-B01	CTGCTGCTATTGCTCATG	137.77442
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29100_Aamu_Bax2006_COI	PLO-B34	1	BD29100_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	GCACAACCGGGATCTTTACTTC	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29100_Pcan_Bot2011_IGS	PLO-B34	1	BD29100_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B34	IGS	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	CGTAATCCTCAGTACTGGCTA	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29100_Pper_Dia2013_COI	PLO-B34	1	BD29100_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	CTTAGTGGCATTAATTCGDAATCC	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29100_Sspa_Oph2007_COI	PLO-B34	1	BD29100_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	GAAGGCCTTCCGATGATC	135.87284
BD29100	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70991	WGS84	BD29100	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29100_Upin_Bot2011_COI	PLO-B34	1	BD29100_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29100	Sample	BD29100	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B34	COI	45	BD29100	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B34	CTTTAATTACAGCGTTTTTATTGTTGT	135.87284
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29101_Cjap_Sim2018_COI	PLO-B33	1	BD29101_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29101_Esin_And2018_Cyb	PLO-B33	1	BD29101_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B33	Cytb	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	ACCCCTCCTCATATCCAACCA	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29101_Mgig_Bot2012_COI	PLO-B33	1	BD29101_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	TCTTATTCGTTGGAGACTTTATAACCCT	135.87046
BD29101	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70996	WGS84	BD29101	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29101_Mjap_Gib2022_COI	PLO-B33	1	BD29101_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29101	Sample	BD29101	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B33	COI	45	BD29101	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B33	AATTTAATTGTGACTAGGCATGGTCTG	135.87046
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29102_Cmae_Bot2011_COI	PLO-B32	1	BD29102_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	ATGAACAGTCTATCCTCCTTTAG	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29102_Hsan_Wil2021_COI	PLO-B32	1	BD29102_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	CCTGGGCCGGTATAGTAGGT	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29102_Mros_Gib2022_COI	PLO-B32	1	BD29102_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29102_Msal_Bot2012_COI	PLO-B32	1	BD29102_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	GYTAGTTCCRATGATGTTAGCTG	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29102_Mstr_Wil2021_COI	PLO-B32	1	BD29102_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	GTAGTGGTAACTACTCATGCTTTA	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29102_Pvir_Dia2013_COI	PLO-B32	1	BD29102_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	CTTAGTGGCATTAATTCGDAATCC	135.86825
BD29102	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.70998	WGS84	BD29102	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29102_Rhar_Sim2018_COI	PLO-B32	1	BD29102_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29102	Sample	BD29102	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B32	COI	45	BD29102	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B32	CTGCTGCTATTGCTCATG	135.86825
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29104_Aamu_Bax2006_COI	PLO-B30	1	BD29104_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	GCACAACCGGGATCTTTACTTC	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29104_Pcan_Bot2011_IGS	PLO-B30	1	BD29104_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B30	IGS	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	CGTAATCCTCAGTACTGGCTA	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29104_Pper_Dia2013_COI	PLO-B30	1	BD29104_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	CTTAGTGGCATTAATTCGDAATCC	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29104_Sspa_Oph2007_COI	PLO-B30	1	BD29104_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	GAAGGCCTTCCGATGATC	135.86831
BD29104	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71319	WGS84	BD29104	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29104_Upin_Bot2011_COI	PLO-B30	1	BD29104_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29104	Sample	BD29104	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B30	COI	45	BD29104	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B30	CTTTAATTACAGCGTTTTTATTGTTGT	135.86831
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29105_Cjap_Sim2018_COI	PLO-B29	1	BD29105_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29105_Esin_And2018_Cyb	PLO-B29	1	BD29105_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B29	Cytb	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	ACCCCTCCTCATATCCAACCA	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29105_Mgig_Bot2012_COI	PLO-B29	1	BD29105_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	TCTTATTCGTTGGAGACTTTATAACCCT	135.86743
BD29105	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29105	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29105_Mjap_Gib2022_COI	PLO-B29	1	BD29105_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29105	Sample	BD29105	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B29	COI	45	BD29105	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B29	AATTTAATTGTGACTAGGCATGGTCTG	135.86743
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29106_Cmae_Bot2011_COI	PLO-B28	1	BD29106_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	ATGAACAGTCTATCCTCCTTTAG	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29106_Hsan_Wil2021_COI	PLO-B28	1	BD29106_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	CCTGGGCCGGTATAGTAGGT	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29106_Mros_Gib2022_COI	PLO-B28	1	BD29106_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29106_Msal_Bot2012_COI	PLO-B28	1	BD29106_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	GYTAGTTCCRATGATGTTAGCTG	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29106_Mstr_Wil2021_COI	PLO-B28	1	BD29106_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	GTAGTGGTAACTACTCATGCTTTA	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29106_Pvir_Dia2013_COI	PLO-B28	1	BD29106_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	CTTAGTGGCATTAATTCGDAATCC	135.86504
BD29106	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71479	WGS84	BD29106	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29106_Rhar_Sim2018_COI	PLO-B28	1	BD29106_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29106	Sample	BD29106	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B28	COI	45	BD29106	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B28	CTGCTGCTATTGCTCATG	135.86504
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29108_Aamu_Bax2006_COI	PLO-B26	1	BD29108_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	GCACAACCGGGATCTTTACTTC	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29108_Pcan_Bot2011_IGS	PLO-B26	1	BD29108_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B26	IGS	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	CGTAATCCTCAGTACTGGCTA	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29108_Pper_Dia2013_COI	PLO-B26	1	BD29108_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	CTTAGTGGCATTAATTCGDAATCC	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29108_Sspa_Oph2007_COI	PLO-B26	1	BD29108_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	GAAGGCCTTCCGATGATC	135.86628
BD29108	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71637	WGS84	BD29108	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29108_Upin_Bot2011_COI	PLO-B26	1	BD29108_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29108	Sample	BD29108	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B26	COI	45	BD29108	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B26	CTTTAATTACAGCGTTTTTATTGTTGT	135.86628
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29109_Cjap_Sim2018_COI	PLO-B25	1	BD29109_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29109_Esin_And2018_Cyb	PLO-B25	1	BD29109_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B25	Cytb	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	ACCCCTCCTCATATCCAACCA	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29109_Mgig_Bot2012_COI	PLO-B25	1	BD29109_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	TCTTATTCGTTGGAGACTTTATAACCCT	135.86842
BD29109	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71633	WGS84	BD29109	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29109_Mjap_Gib2022_COI	PLO-B25	1	BD29109_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29109	Sample	BD29109	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B25	COI	45	BD29109	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B25	AATTTAATTGTGACTAGGCATGGTCTG	135.86842
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29111_Aamu_Bax2006_COI	PLO-B23	1	BD29111_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	GCACAACCGGGATCTTTACTTC	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29111_Pcan_Bot2011_IGS	PLO-B23	1	BD29111_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B23	IGS	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	CGTAATCCTCAGTACTGGCTA	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29111_Pper_Dia2013_COI	PLO-B23	1	BD29111_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	CTTAGTGGCATTAATTCGDAATCC	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29111_Sspa_Oph2007_COI	PLO-B23	1	BD29111_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	GAAGGCCTTCCGATGATC	135.86511
BD29111	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71795	WGS84	BD29111	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29111_Upin_Bot2011_COI	PLO-B23	1	BD29111_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29111	Sample	BD29111	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B23	COI	45	BD29111	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B23	CTTTAATTACAGCGTTTTTATTGTTGT	135.86511
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71962	WGS84	BD29112	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29112_Asen_Wil2025_COI	PLO-B22	1	BD29112_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	GTTGTTGTTACAACACATGCTCTTTTG	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29112_Cjap_Sim2018_COI	PLO-B22	1	BD29112_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29112_Esin_And2018_Cyb	PLO-B22	1	BD29112_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B22	Cytb	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	ACCCCTCCTCATATCCAACCA	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29112_Mgig_Bot2012_COI	PLO-B22	1	BD29112_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	TCTTATTCGTTGGAGACTTTATAACCCT	135.86435
BD29112	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71962	WGS84	BD29112	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29112_Mjap_Gib2022_COI	PLO-B22	1	BD29112_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29112	Sample	BD29112	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B22	COI	45	BD29112	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B22	AATTTAATTGTGACTAGGCATGGTCTG	135.86435
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29113_Cmae_Bot2011_COI	PLO-B21	1	BD29113_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	ATGAACAGTCTATCCTCCTTTAG	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29113_Hsan_Wil2021_COI	PLO-B21	1	BD29113_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	CCTGGGCCGGTATAGTAGGT	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29113_Mros_Gib2022_COI	PLO-B21	1	BD29113_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29113_Msal_Bot2012_COI	PLO-B21	1	BD29113_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	GYTAGTTCCRATGATGTTAGCTG	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29113_Mstr_Wil2021_COI	PLO-B21	1	BD29113_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	GTAGTGGTAACTACTCATGCTTTA	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29113_Pvir_Dia2013_COI	PLO-B21	1	BD29113_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	CTTAGTGGCATTAATTCGDAATCC	135.86744
BD29113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71159	WGS84	BD29113	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29113_Rhar_Sim2018_COI	PLO-B21	1	BD29113_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29113	Sample	BD29113	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B21	COI	45	BD29113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B21	CTGCTGCTATTGCTCATG	135.86744
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29115_Aamu_Bax2006_COI	PLO-B19	1	BD29115_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	GCACAACCGGGATCTTTACTTC	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29115_Pcan_Bot2011_IGS	PLO-B19	1	BD29115_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B19	IGS	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	CGTAATCCTCAGTACTGGCTA	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29115_Pper_Dia2013_COI	PLO-B19	1	BD29115_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	CTTAGTGGCATTAATTCGDAATCC	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29115_Sspa_Oph2007_COI	PLO-B19	1	BD29115_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	GAAGGCCTTCCGATGATC	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71152	WGS84	BD29115	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29115_Upin_Bot2011_COI	PLO-B19	1	BD29115_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	CTTTAATTACAGCGTTTTTATTGTTGT	135.87177
BD29115	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71152	WGS84	BD29115	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29115_Vgib_Wil2025_COI	PLO-B19	1	BD29115_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29115	Sample	BD29115	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B19	COI	45	BD29115	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B19	CCCCTCTTTACAAGTTCTTTAGGGT	135.87177
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71148	WGS84	BD29116	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29116_Asen_Wil2025_COI	PLO-B18	1	BD29116_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Asen_Wil2025_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	GTTGTTGTTACAACACATGCTCTTTTG	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29116_Cjap_Sim2018_COI	PLO-B18	1	BD29116_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29116_Esin_And2018_Cyb	PLO-B18	1	BD29116_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B18	Cytb	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	ACCCCTCCTCATATCCAACCA	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29116_Mgig_Bot2012_COI	PLO-B18	1	BD29116_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	TCTTATTCGTTGGAGACTTTATAACCCT	135.87388
BD29116	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71148	WGS84	BD29116	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29116_Mjap_Gib2022_COI	PLO-B18	1	BD29116_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29116	Sample	BD29116	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B18	COI	45	BD29116	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B18	AATTTAATTGTGACTAGGCATGGTCTG	135.87388
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29117_Cmae_Bot2011_COI	PLO-B17	1	BD29117_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	ATGAACAGTCTATCCTCCTTTAG	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29117_Hsan_Wil2021_COI	PLO-B17	1	BD29117_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	CCTGGGCCGGTATAGTAGGT	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29117_Mros_Gib2022_COI	PLO-B17	1	BD29117_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29117_Msal_Bot2012_COI	PLO-B17	1	BD29117_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	GYTAGTTCCRATGATGTTAGCTG	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29117_Mstr_Wil2021_COI	PLO-B17	1	BD29117_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	GTAGTGGTAACTACTCATGCTTTA	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29117_Pvir_Dia2013_COI	PLO-B17	1	BD29117_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	CTTAGTGGCATTAATTCGDAATCC	135.87506
BD29117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71303	WGS84	BD29117	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29117_Rhar_Sim2018_COI	PLO-B17	1	BD29117_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29117	Sample	BD29117	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B17	COI	45	BD29117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B17	CTGCTGCTATTGCTCATG	135.87506
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29119_Aamu_Bax2006_COI	PLO-B15	1	BD29119_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	GCACAACCGGGATCTTTACTTC	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29119_Pcan_Bot2011_IGS	PLO-B15	1	BD29119_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B15	IGS	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	CGTAATCCTCAGTACTGGCTA	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29119_Pper_Dia2013_COI	PLO-B15	1	BD29119_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	CTTAGTGGCATTAATTCGDAATCC	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29119_Sspa_Oph2007_COI	PLO-B15	1	BD29119_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	GAAGGCCTTCCGATGATC	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71468	WGS84	BD29119	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29119_Upin_Bot2011_COI	PLO-B15	1	BD29119_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	CTTTAATTACAGCGTTTTTATTGTTGT	135.87184
BD29119	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16577	-34.71468	WGS84	BD29119	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29119_Vgib_Wil2025_COI	PLO-B15	1	BD29119_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29119	Sample	BD29119	targeted	Absent	Sample	Vgib_Wil2025_COI	PLO-B15	COI	45	BD29119	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B15	CCCCTCTTTACAAGTTCTTTAGGGT	135.87184
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29120_Cmae_Bot2011_COI	PLO-B14	1	BD29120_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	ATGAACAGTCTATCCTCCTTTAG	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29120_Hsan_Wil2021_COI	PLO-B14	1	BD29120_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	CCTGGGCCGGTATAGTAGGT	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29120_Mros_Gib2022_COI	PLO-B14	1	BD29120_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29120_Msal_Bot2012_COI	PLO-B14	1	BD29120_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	GYTAGTTCCRATGATGTTAGCTG	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29120_Mstr_Wil2021_COI	PLO-B14	1	BD29120_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	GTAGTGGTAACTACTCATGCTTTA	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29120_Pvir_Dia2013_COI	PLO-B14	1	BD29120_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	CTTAGTGGCATTAATTCGDAATCC	135.87404
BD29120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.7147	WGS84	BD29120	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29120_Rhar_Sim2018_COI	PLO-B14	1	BD29120_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29120	Sample	BD29120	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B14	COI	45	BD29120	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B14	CTGCTGCTATTGCTCATG	135.87404
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29122_Aamu_Bax2006_COI	PLO-B12	1	BD29122_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	GCACAACCGGGATCTTTACTTC	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29122_Pcan_Bot2011_IGS	PLO-B12	1	BD29122_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B12	IGS	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	CGTAATCCTCAGTACTGGCTA	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29122_Pper_Dia2013_COI	PLO-B12	1	BD29122_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	CTTAGTGGCATTAATTCGDAATCC	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29122_Sspa_Oph2007_COI	PLO-B12	1	BD29122_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	GAAGGCCTTCCGATGATC	135.87842
BD29122	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71463	WGS84	BD29122	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29122_Upin_Bot2011_COI	PLO-B12	1	BD29122_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29122	Sample	BD29122	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B12	COI	45	BD29122	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B12	CTTTAATTACAGCGTTTTTATTGTTGT	135.87842
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29123_Cjap_Sim2018_COI	PLO-B11	1	BD29123_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29123_Esin_And2018_Cyb	PLO-B11	1	BD29123_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B11	Cytb	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	ACCCCTCCTCATATCCAACCA	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29123_Mgig_Bot2012_COI	PLO-B11	1	BD29123_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	TCTTATTCGTTGGAGACTTTATAACCCT	135.87959
BD29123	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71615	WGS84	BD29123	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29123_Mjap_Gib2022_COI	PLO-B11	1	BD29123_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29123	Sample	BD29123	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B11	COI	45	BD29123	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B11	AATTTAATTGTGACTAGGCATGGTCTG	135.87959
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29124_Cmae_Bot2011_COI	PLO-B10	1	BD29124_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	ATGAACAGTCTATCCTCCTTTAG	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29124_Hsan_Wil2021_COI	PLO-B10	1	BD29124_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	CCTGGGCCGGTATAGTAGGT	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29124_Mros_Gib2022_COI	PLO-B10	1	BD29124_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29124_Msal_Bot2012_COI	PLO-B10	1	BD29124_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	GYTAGTTCCRATGATGTTAGCTG	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29124_Mstr_Wil2021_COI	PLO-B10	1	BD29124_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	GTAGTGGTAACTACTCATGCTTTA	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29124_Pvir_Dia2013_COI	PLO-B10	1	BD29124_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	CTTAGTGGCATTAATTCGDAATCC	135.87733
BD29124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71622	WGS84	BD29124	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29124_Rhar_Sim2018_COI	PLO-B10	1	BD29124_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29124	Sample	BD29124	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B10	COI	45	BD29124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B10	CTGCTGCTATTGCTCATG	135.87733
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29126_Aamu_Bax2006_COI	PLO-B08	1	BD29126_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	GCACAACCGGGATCTTTACTTC	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29126_Pcan_Bot2011_IGS	PLO-B08	1	BD29126_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B08	IGS	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	CGTAATCCTCAGTACTGGCTA	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29126_Pper_Dia2013_COI	PLO-B08	1	BD29126_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	CTTAGTGGCATTAATTCGDAATCC	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29126_Sspa_Oph2007_COI	PLO-B08	1	BD29126_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	GAAGGCCTTCCGATGATC	135.87292
BD29126	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71624	WGS84	BD29126	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29126_Upin_Bot2011_COI	PLO-B08	1	BD29126_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29126	Sample	BD29126	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B08	COI	45	BD29126	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B08	CTTTAATTACAGCGTTTTTATTGTTGT	135.87292
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29127_Cjap_Sim2018_COI	PLO-B07	1	BD29127_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29127_Esin_And2018_Cyb	PLO-B07	1	BD29127_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B07	Cytb	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	ACCCCTCCTCATATCCAACCA	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29127_Mgig_Bot2012_COI	PLO-B07	1	BD29127_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	TCTTATTCGTTGGAGACTTTATAACCCT	135.8712
BD29127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71644	WGS84	BD29127	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29127_Mjap_Gib2022_COI	PLO-B07	1	BD29127_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29127	Sample	BD29127	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B07	COI	45	BD29127	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B07	AATTTAATTGTGACTAGGCATGGTCTG	135.8712
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29128_Cmae_Bot2011_COI	PLO-B06	1	BD29128_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	ATGAACAGTCTATCCTCCTTTAG	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29128_Hsan_Wil2021_COI	PLO-B06	1	BD29128_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	CCTGGGCCGGTATAGTAGGT	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29128_Mros_Gib2022_COI	PLO-B06	1	BD29128_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29128_Msal_Bot2012_COI	PLO-B06	1	BD29128_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	GYTAGTTCCRATGATGTTAGCTG	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29128_Mstr_Wil2021_COI	PLO-B06	1	BD29128_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	GTAGTGGTAACTACTCATGCTTTA	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29128_Pvir_Dia2013_COI	PLO-B06	1	BD29128_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	CTTAGTGGCATTAATTCGDAATCC	135.8719
BD29128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71789	WGS84	BD29128	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29128_Rhar_Sim2018_COI	PLO-B06	1	BD29128_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29128	Sample	BD29128	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B06	COI	45	BD29128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B06	CTGCTGCTATTGCTCATG	135.8719
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29130_Cjap_Sim2018_COI	PLO-B04	1	BD29130_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29130_Esin_And2018_Cyb	PLO-B04	1	BD29130_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-B04	Cytb	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	ACCCCTCCTCATATCCAACCA	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29130_Mgig_Bot2012_COI	PLO-B04	1	BD29130_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	TCTTATTCGTTGGAGACTTTATAACCCT	135.87408
BD29130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71783	WGS84	BD29130	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29130_Mjap_Gib2022_COI	PLO-B04	1	BD29130_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29130	Sample	BD29130	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-B04	COI	45	BD29130	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B04	AATTTAATTGTGACTAGGCATGGTCTG	135.87408
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29131_Cmae_Bot2011_COI	PLO-B03	1	BD29131_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	ATGAACAGTCTATCCTCCTTTAG	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29131_Hsan_Wil2021_COI	PLO-B03	1	BD29131_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	CCTGGGCCGGTATAGTAGGT	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29131_Mros_Gib2022_COI	PLO-B03	1	BD29131_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29131_Msal_Bot2012_COI	PLO-B03	1	BD29131_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	GYTAGTTCCRATGATGTTAGCTG	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29131_Mstr_Wil2021_COI	PLO-B03	1	BD29131_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	GTAGTGGTAACTACTCATGCTTTA	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29131_Pvir_Dia2013_COI	PLO-B03	1	BD29131_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	CTTAGTGGCATTAATTCGDAATCC	135.87627
BD29131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71774	WGS84	BD29131	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29131_Rhar_Sim2018_COI	PLO-B03	1	BD29131_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29131	Sample	BD29131	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-B03	COI	45	BD29131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B03	CTGCTGCTATTGCTCATG	135.87627
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29133_Aamu_Bax2006_COI	PLO-B01	1	BD29133_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	GCACAACCGGGATCTTTACTTC	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29133_Pcan_Bot2011_IGS	PLO-B01	1	BD29133_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-B01	IGS	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	CGTAATCCTCAGTACTGGCTA	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29133_Pper_Dia2013_COI	PLO-B01	1	BD29133_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	CTTAGTGGCATTAATTCGDAATCC	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29133_Sspa_Oph2007_COI	PLO-B01	1	BD29133_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	GAAGGCCTTCCGATGATC	135.88088
BD29133	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14702	-34.71776	WGS84	BD29133	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	15/02/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29133_Upin_Bot2011_COI	PLO-B01	1	BD29133_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29133	Sample	BD29133	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-B01	COI	45	BD29133	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-B01	CTTTAATTACAGCGTTTTTATTGTTGT	135.88088
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29134_Cjap_Sim2018_COI	ADL-A70	1	BD29134_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29134_Esin_And2018_Cyb	ADL-A70	1	BD29134_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A70	Cytb	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	ACCCCTCCTCATATCCAACCA	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	37.16	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29134_Mgig_Bot2012_COI	ADL-A70	1	BD29134_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	TCTTATTCGTTGGAGACTTTATAACCCT	138.51415
BD29134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81115	WGS84	BD29134	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29134_Mjap_Gib2022_COI	ADL-A70	1	BD29134_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29134	Sample	BD29134	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A70	COI	45	BD29134	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A70	AATTTAATTGTGACTAGGCATGGTCTG	138.51415
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	27.234	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29135_Cmae_Bot2011_COI	ADL-A69	1	BD29135_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	ATGAACAGTCTATCCTCCTTTAG	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29135_Hsan_Wil2021_COI	ADL-A69	1	BD29135_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	CCTGGGCCGGTATAGTAGGT	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29135_Mros_Gib2022_COI	ADL-A69	1	BD29135_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29135_Msal_Bot2012_COI	ADL-A69	1	BD29135_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	GYTAGTTCCRATGATGTTAGCTG	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29135_Mstr_Wil2021_COI	ADL-A69	1	BD29135_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	GTAGTGGTAACTACTCATGCTTTA	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29135_Pvir_Dia2013_COI	ADL-A69	1	BD29135_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	CTTAGTGGCATTAATTCGDAATCC	138.51371
BD29135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81219	WGS84	BD29135	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29135_Rhar_Sim2018_COI	ADL-A69	1	BD29135_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29135	Sample	BD29135	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A69	COI	45	BD29135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A69	CTGCTGCTATTGCTCATG	138.51371
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29137_Aamu_Bax2006_COI	ADL-A67	1	BD29137_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	GCACAACCGGGATCTTTACTTC	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29137_Pcan_Bot2011_IGS	ADL-A67	1	BD29137_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A67	IGS	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	CGTAATCCTCAGTACTGGCTA	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29137_Pper_Dia2013_COI	ADL-A67	1	BD29137_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	CTTAGTGGCATTAATTCGDAATCC	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.207	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29137_Sspa_Oph2007_COI	ADL-A67	1	BD29137_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	GAAGGCCTTCCGATGATC	138.51305
BD29137	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8153	WGS84	BD29137	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29137_Upin_Bot2011_COI	ADL-A67	1	BD29137_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29137	Sample	BD29137	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A67	COI	45	BD29137	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A67	CTTTAATTACAGCGTTTTTATTGTTGT	138.51305
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29138_Cjap_Sim2018_COI	ADL-A66	1	BD29138_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29138_Esin_And2018_Cyb	ADL-A66	1	BD29138_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A66	Cytb	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	ACCCCTCCTCATATCCAACCA	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29138_Mgig_Bot2012_COI	ADL-A66	1	BD29138_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	TCTTATTCGTTGGAGACTTTATAACCCT	138.51382
BD29138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81621	WGS84	BD29138	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29138_Mjap_Gib2022_COI	ADL-A66	1	BD29138_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29138	Sample	BD29138	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A66	COI	45	BD29138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A66	AATTTAATTGTGACTAGGCATGGTCTG	138.51382
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	25.039	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29139_Cmae_Bot2011_COI	ADL-A65	1	BD29139_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	ATGAACAGTCTATCCTCCTTTAG	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29139_Hsan_Wil2021_COI	ADL-A65	1	BD29139_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	CCTGGGCCGGTATAGTAGGT	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29139_Mros_Gib2022_COI	ADL-A65	1	BD29139_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29139_Msal_Bot2012_COI	ADL-A65	1	BD29139_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	GYTAGTTCCRATGATGTTAGCTG	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29139_Mstr_Wil2021_COI	ADL-A65	1	BD29139_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	GTAGTGGTAACTACTCATGCTTTA	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29139_Pvir_Dia2013_COI	ADL-A65	1	BD29139_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	CTTAGTGGCATTAATTCGDAATCC	138.51317
BD29139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81728	WGS84	BD29139	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29139_Rhar_Sim2018_COI	ADL-A65	1	BD29139_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29139	Sample	BD29139	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A65	COI	45	BD29139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A65	CTGCTGCTATTGCTCATG	138.51317
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29140_Aamu_Bax2006_COI	ADL-A64	1	BD29140_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	GCACAACCGGGATCTTTACTTC	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29140_Pcan_Bot2011_IGS	ADL-A64	1	BD29140_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A64	IGS	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	CGTAATCCTCAGTACTGGCTA	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29140_Pper_Dia2013_COI	ADL-A64	1	BD29140_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	CTTAGTGGCATTAATTCGDAATCC	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.458	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29140_Sspa_Oph2007_COI	ADL-A64	1	BD29140_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	GAAGGCCTTCCGATGATC	138.51268
BD29140	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81844	WGS84	BD29140	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29140_Upin_Bot2011_COI	ADL-A64	1	BD29140_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29140	Sample	BD29140	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A64	COI	45	BD29140	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A64	CTTTAATTACAGCGTTTTTATTGTTGT	138.51268
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29141_Cjap_Sim2018_COI	ADL-A63	1	BD29141_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29141_Esin_And2018_Cyb	ADL-A63	1	BD29141_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A63	Cytb	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	ACCCCTCCTCATATCCAACCA	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29141_Mgig_Bot2012_COI	ADL-A63	1	BD29141_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	TCTTATTCGTTGGAGACTTTATAACCCT	138.51165
BD29141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.81988	WGS84	BD29141	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29141_Mjap_Gib2022_COI	ADL-A63	1	BD29141_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29141	Sample	BD29141	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A63	COI	45	BD29141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A63	AATTTAATTGTGACTAGGCATGGTCTG	138.51165
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29142_Cmae_Bot2011_COI	ADL-A62	1	BD29142_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	ATGAACAGTCTATCCTCCTTTAG	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29142_Hsan_Wil2021_COI	ADL-A62	1	BD29142_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	CCTGGGCCGGTATAGTAGGT	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29142_Mros_Gib2022_COI	ADL-A62	1	BD29142_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29142_Msal_Bot2012_COI	ADL-A62	1	BD29142_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	GYTAGTTCCRATGATGTTAGCTG	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29142_Pvir_Dia2013_COI	ADL-A62	1	BD29142_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	CTTAGTGGCATTAATTCGDAATCC	138.51129
BD29142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82043	WGS84	BD29142	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29142_Rhar_Sim2018_COI	ADL-A62	1	BD29142_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29142	Sample	BD29142	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A62	COI	45	BD29142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A62	CTGCTGCTATTGCTCATG	138.51129
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29144_Aamu_Bax2006_COI	ADL-A60	1	BD29144_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	GCACAACCGGGATCTTTACTTC	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29144_Pcan_Bot2011_IGS	ADL-A60	1	BD29144_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A60	IGS	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	CGTAATCCTCAGTACTGGCTA	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29144_Pper_Dia2013_COI	ADL-A60	1	BD29144_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	CTTAGTGGCATTAATTCGDAATCC	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.505	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29144_Sspa_Oph2007_COI	ADL-A60	1	BD29144_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	GAAGGCCTTCCGATGATC	138.5106
BD29144	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82147	WGS84	BD29144	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29144_Upin_Bot2011_COI	ADL-A60	1	BD29144_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29144	Sample	BD29144	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A60	COI	45	BD29144	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A60	CTTTAATTACAGCGTTTTTATTGTTGT	138.5106
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29145_Cjap_Sim2018_COI	ADL-A59	1	BD29145_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29145_Esin_And2018_Cyb	ADL-A59	1	BD29145_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A59	Cytb	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	ACCCCTCCTCATATCCAACCA	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29145_Mgig_Bot2012_COI	ADL-A59	1	BD29145_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	TCTTATTCGTTGGAGACTTTATAACCCT	138.51145
BD29145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82246	WGS84	BD29145	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29145_Mjap_Gib2022_COI	ADL-A59	1	BD29145_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29145	Sample	BD29145	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A59	COI	45	BD29145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A59	AATTTAATTGTGACTAGGCATGGTCTG	138.51145
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.29	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29146_Cmae_Bot2011_COI	ADL-A58	1	BD29146_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	ATGAACAGTCTATCCTCCTTTAG	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29146_Hsan_Wil2021_COI	ADL-A58	1	BD29146_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	CCTGGGCCGGTATAGTAGGT	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29146_Mros_Gib2022_COI	ADL-A58	1	BD29146_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29146_Msal_Bot2012_COI	ADL-A58	1	BD29146_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	GYTAGTTCCRATGATGTTAGCTG	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29146_Mstr_Wil2021_COI	ADL-A58	1	BD29146_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	GTAGTGGTAACTACTCATGCTTTA	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29146_Pvir_Dia2013_COI	ADL-A58	1	BD29146_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	CTTAGTGGCATTAATTCGDAATCC	138.51002
BD29146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82251	WGS84	BD29146	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29146_Rhar_Sim2018_COI	ADL-A58	1	BD29146_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29146	Sample	BD29146	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A58	COI	45	BD29146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A58	CTGCTGCTATTGCTCATG	138.51002
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29148_Aamu_Bax2006_COI	ADL-A56	1	BD29148_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	GCACAACCGGGATCTTTACTTC	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29148_Pcan_Bot2011_IGS	ADL-A56	1	BD29148_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A56	IGS	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	CGTAATCCTCAGTACTGGCTA	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29148_Pper_Dia2013_COI	ADL-A56	1	BD29148_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	CTTAGTGGCATTAATTCGDAATCC	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	32.049	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29148_Sspa_Oph2007_COI	ADL-A56	1	BD29148_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	GAAGGCCTTCCGATGATC	138.50936
BD29148	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82357	WGS84	BD29148	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29148_Upin_Bot2011_COI	ADL-A56	1	BD29148_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29148	Sample	BD29148	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A56	COI	45	BD29148	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A56	CTTTAATTACAGCGTTTTTATTGTTGT	138.50936
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29149_Cjap_Sim2018_COI	ADL-A55	1	BD29149_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29149_Esin_And2018_Cyb	ADL-A55	1	BD29149_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A55	Cytb	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	ACCCCTCCTCATATCCAACCA	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29149_Mgig_Bot2012_COI	ADL-A55	1	BD29149_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	TCTTATTCGTTGGAGACTTTATAACCCT	138.51001
BD29149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82454	WGS84	BD29149	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29149_Mjap_Gib2022_COI	ADL-A55	1	BD29149_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29149	Sample	BD29149	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A55	COI	45	BD29149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A55	AATTTAATTGTGACTAGGCATGGTCTG	138.51001
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29151_Aamu_Bax2006_COI	ADL-A53	1	BD29151_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	GCACAACCGGGATCTTTACTTC	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29151_Pcan_Bot2011_IGS	ADL-A53	1	BD29151_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A53	IGS	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	CGTAATCCTCAGTACTGGCTA	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29151_Pper_Dia2013_COI	ADL-A53	1	BD29151_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	CTTAGTGGCATTAATTCGDAATCC	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.918	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29151_Sspa_Oph2007_COI	ADL-A53	1	BD29151_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	GAAGGCCTTCCGATGATC	138.5094
BD29151	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82561	WGS84	BD29151	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29151_Upin_Bot2011_COI	ADL-A53	1	BD29151_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29151	Sample	BD29151	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A53	COI	45	BD29151	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A53	CTTTAATTACAGCGTTTTTATTGTTGT	138.5094
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29152_Cjap_Sim2018_COI	ADL-A52	1	BD29152_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29152_Esin_And2018_Cyb	ADL-A52	1	BD29152_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A52	Cytb	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	ACCCCTCCTCATATCCAACCA	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29152_Mgig_Bot2012_COI	ADL-A52	1	BD29152_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	TCTTATTCGTTGGAGACTTTATAACCCT	138.50802
BD29152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.8256	WGS84	BD29152	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29152_Mjap_Gib2022_COI	ADL-A52	1	BD29152_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29152	Sample	BD29152	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A52	COI	45	BD29152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A52	AATTTAATTGTGACTAGGCATGGTCTG	138.50802
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29153_Cmae_Bot2011_COI	ADL-A51	1	BD29153_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	ATGAACAGTCTATCCTCCTTTAG	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29153_Hsan_Wil2021_COI	ADL-A51	1	BD29153_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	CCTGGGCCGGTATAGTAGGT	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29153_Mros_Gib2022_COI	ADL-A51	1	BD29153_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29153_Msal_Bot2012_COI	ADL-A51	1	BD29153_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	GYTAGTTCCRATGATGTTAGCTG	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29153_Mstr_Wil2021_COI	ADL-A51	1	BD29153_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	GTAGTGGTAACTACTCATGCTTTA	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29153_Pvir_Dia2013_COI	ADL-A51	1	BD29153_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	CTTAGTGGCATTAATTCGDAATCC	138.50876
BD29153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82661	WGS84	BD29153	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29153_Rhar_Sim2018_COI	ADL-A51	1	BD29153_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29153	Sample	BD29153	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A51	COI	45	BD29153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A51	CTGCTGCTATTGCTCATG	138.50876
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29155_Aamu_Bax2006_COI	ADL-A49	1	BD29155_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	GCACAACCGGGATCTTTACTTC	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29155_Pcan_Bot2011_IGS	ADL-A49	1	BD29155_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A49	IGS	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	CGTAATCCTCAGTACTGGCTA	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29155_Pper_Dia2013_COI	ADL-A49	1	BD29155_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	CTTAGTGGCATTAATTCGDAATCC	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.949	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29155_Sspa_Oph2007_COI	ADL-A49	1	BD29155_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	GAAGGCCTTCCGATGATC	138.50794
BD29155	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82769	WGS84	BD29155	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29155_Upin_Bot2011_COI	ADL-A49	1	BD29155_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29155	Sample	BD29155	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A49	COI	45	BD29155	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A49	CTTTAATTACAGCGTTTTTATTGTTGT	138.50794
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29156_Cjap_Sim2018_COI	ADL-A48	1	BD29156_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29156_Esin_And2018_Cyb	ADL-A48	1	BD29156_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A48	Cytb	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	ACCCCTCCTCATATCCAACCA	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	36.438	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29156_Mgig_Bot2012_COI	ADL-A48	1	BD29156_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Present	Sample	Mgig_Bot2012_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	TCTTATTCGTTGGAGACTTTATAACCCT	138.50888
BD29156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82867	WGS84	BD29156	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29156_Mjap_Gib2022_COI	ADL-A48	1	BD29156_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29156	Sample	BD29156	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A48	COI	45	BD29156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A48	AATTTAATTGTGACTAGGCATGGTCTG	138.50888
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	38.872	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29157_Cmae_Bot2011_COI	ADL-A47	1	BD29157_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	ATGAACAGTCTATCCTCCTTTAG	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29157_Hsan_Wil2021_COI	ADL-A47	1	BD29157_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	CCTGGGCCGGTATAGTAGGT	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29157_Mros_Gib2022_COI	ADL-A47	1	BD29157_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29157_Msal_Bot2012_COI	ADL-A47	1	BD29157_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	GYTAGTTCCRATGATGTTAGCTG	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29157_Mstr_Wil2021_COI	ADL-A47	1	BD29157_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	GTAGTGGTAACTACTCATGCTTTA	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29157_Pvir_Dia2013_COI	ADL-A47	1	BD29157_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	CTTAGTGGCATTAATTCGDAATCC	138.50732
BD29157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82874	WGS84	BD29157	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29157_Rhar_Sim2018_COI	ADL-A47	1	BD29157_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29157	Sample	BD29157	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A47	COI	45	BD29157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A47	CTGCTGCTATTGCTCATG	138.50732
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29159_Aamu_Bax2006_COI	ADL-A45	1	BD29159_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	GCACAACCGGGATCTTTACTTC	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29159_Pcan_Bot2011_IGS	ADL-A45	1	BD29159_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A45	IGS	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	CGTAATCCTCAGTACTGGCTA	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29159_Pper_Dia2013_COI	ADL-A45	1	BD29159_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	CTTAGTGGCATTAATTCGDAATCC	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.151	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29159_Sspa_Oph2007_COI	ADL-A45	1	BD29159_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	GAAGGCCTTCCGATGATC	138.50813
BD29159	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.82976	WGS84	BD29159	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29159_Upin_Bot2011_COI	ADL-A45	1	BD29159_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29159	Sample	BD29159	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A45	COI	45	BD29159	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A45	CTTTAATTACAGCGTTTTTATTGTTGT	138.50813
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29160_Cmae_Bot2011_COI	ADL-A44	1	BD29160_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	ATGAACAGTCTATCCTCCTTTAG	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29160_Hsan_Wil2021_COI	ADL-A44	1	BD29160_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	CCTGGGCCGGTATAGTAGGT	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29160_Mros_Gib2022_COI	ADL-A44	1	BD29160_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29160_Msal_Bot2012_COI	ADL-A44	1	BD29160_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	GYTAGTTCCRATGATGTTAGCTG	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29160_Mstr_Wil2021_COI	ADL-A44	1	BD29160_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	GTAGTGGTAACTACTCATGCTTTA	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29160_Pvir_Dia2013_COI	ADL-A44	1	BD29160_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	CTTAGTGGCATTAATTCGDAATCC	138.50737
BD29160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83073	WGS84	BD29160	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29160_Rhar_Sim2018_COI	ADL-A44	1	BD29160_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29160	Sample	BD29160	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A44	COI	45	BD29160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A44	CTGCTGCTATTGCTCATG	138.50737
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29162_Aamu_Bax2006_COI	ADL-A42	1	BD29162_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	GCACAACCGGGATCTTTACTTC	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29162_Pcan_Bot2011_IGS	ADL-A42	1	BD29162_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A42	IGS	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	CGTAATCCTCAGTACTGGCTA	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29162_Pper_Dia2013_COI	ADL-A42	1	BD29162_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	CTTAGTGGCATTAATTCGDAATCC	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.392	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29162_Sspa_Oph2007_COI	ADL-A42	1	BD29162_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	GAAGGCCTTCCGATGATC	138.50687
BD29162	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83584	WGS84	BD29162	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29162_Upin_Bot2011_COI	ADL-A42	1	BD29162_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29162	Sample	BD29162	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A42	COI	45	BD29162	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A42	CTTTAATTACAGCGTTTTTATTGTTGT	138.50687
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29163_Cjap_Sim2018_COI	ADL-A41	1	BD29163_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29163_Esin_And2018_Cyb	ADL-A41	1	BD29163_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A41	Cytb	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	ACCCCTCCTCATATCCAACCA	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29163_Mgig_Bot2012_COI	ADL-A41	1	BD29163_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	TCTTATTCGTTGGAGACTTTATAACCCT	138.50654
BD29163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83892	WGS84	BD29163	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29163_Mjap_Gib2022_COI	ADL-A41	1	BD29163_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29163	Sample	BD29163	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A41	COI	45	BD29163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A41	AATTTAATTGTGACTAGGCATGGTCTG	138.50654
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29164_Cmae_Bot2011_COI	ADL-A40	1	BD29164_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	ATGAACAGTCTATCCTCCTTTAG	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29164_Hsan_Wil2021_COI	ADL-A40	1	BD29164_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	CCTGGGCCGGTATAGTAGGT	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29164_Mros_Gib2022_COI	ADL-A40	1	BD29164_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29164_Msal_Bot2012_COI	ADL-A40	1	BD29164_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	GYTAGTTCCRATGATGTTAGCTG	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29164_Mstr_Wil2021_COI	ADL-A40	1	BD29164_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	GTAGTGGTAACTACTCATGCTTTA	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29164_Pvir_Dia2013_COI	ADL-A40	1	BD29164_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	CTTAGTGGCATTAATTCGDAATCC	138.50533
BD29164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.83909	WGS84	BD29164	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29164_Rhar_Sim2018_COI	ADL-A40	1	BD29164_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29164	Sample	BD29164	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A40	COI	45	BD29164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A40	CTGCTGCTATTGCTCATG	138.50533
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29166_Aamu_Bax2006_COI	ADL-A38	1	BD29166_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	GCACAACCGGGATCTTTACTTC	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29166_Pcan_Bot2011_IGS	ADL-A38	1	BD29166_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A38	IGS	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	CGTAATCCTCAGTACTGGCTA	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29166_Pper_Dia2013_COI	ADL-A38	1	BD29166_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	CTTAGTGGCATTAATTCGDAATCC	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.998	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29166_Sspa_Oph2007_COI	ADL-A38	1	BD29166_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	GAAGGCCTTCCGATGATC	138.50531
BD29166	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84104	WGS84	BD29166	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29166_Upin_Bot2011_COI	ADL-A38	1	BD29166_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29166	Sample	BD29166	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A38	COI	45	BD29166	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A38	CTTTAATTACAGCGTTTTTATTGTTGT	138.50531
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29167_Cjap_Sim2018_COI	ADL-A37	1	BD29167_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29167_Esin_And2018_Cyb	ADL-A37	1	BD29167_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A37	Cytb	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	ACCCCTCCTCATATCCAACCA	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29167_Mgig_Bot2012_COI	ADL-A37	1	BD29167_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	TCTTATTCGTTGGAGACTTTATAACCCT	138.50054
BD29167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84239	WGS84	BD29167	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29167_Mjap_Gib2022_COI	ADL-A37	1	BD29167_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29167	Sample	BD29167	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A37	COI	45	BD29167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A37	AATTTAATTGTGACTAGGCATGGTCTG	138.50054
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29168_Cmae_Bot2011_COI	ADL-A36	1	BD29168_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	ATGAACAGTCTATCCTCCTTTAG	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29168_Hsan_Wil2021_COI	ADL-A36	1	BD29168_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	CCTGGGCCGGTATAGTAGGT	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29168_Mros_Gib2022_COI	ADL-A36	1	BD29168_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29168_Msal_Bot2012_COI	ADL-A36	1	BD29168_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	GYTAGTTCCRATGATGTTAGCTG	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29168_Mstr_Wil2021_COI	ADL-A36	1	BD29168_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	GTAGTGGTAACTACTCATGCTTTA	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29168_Pvir_Dia2013_COI	ADL-A36	1	BD29168_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	CTTAGTGGCATTAATTCGDAATCC	138.4992
BD29168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.84255	WGS84	BD29168	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Inner_Harbor_Winter	27/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29168_Rhar_Sim2018_COI	ADL-A36	1	BD29168_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29168	Sample	BD29168	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A36	COI	45	BD29168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A36	CTGCTGCTATTGCTCATG	138.4992
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29170_Cjap_Sim2018_COI	ADL-A34	1	BD29170_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29170_Esin_And2018_Cyb	ADL-A34	1	BD29170_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A34	Cytb	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	ACCCCTCCTCATATCCAACCA	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29170_Mgig_Bot2012_COI	ADL-A34	1	BD29170_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	TCTTATTCGTTGGAGACTTTATAACCCT	138.49805
BD29170	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76083	WGS84	BD29170	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29170_Mjap_Gib2022_COI	ADL-A34	1	BD29170_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29170	Sample	BD29170	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A34	COI	45	BD29170	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A34	AATTTAATTGTGACTAGGCATGGTCTG	138.49805
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29171_Cmae_Bot2011_COI	ADL-A33	1	BD29171_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	ATGAACAGTCTATCCTCCTTTAG	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29171_Hsan_Wil2021_COI	ADL-A33	1	BD29171_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	CCTGGGCCGGTATAGTAGGT	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29171_Mros_Gib2022_COI	ADL-A33	1	BD29171_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29171_Msal_Bot2012_COI	ADL-A33	1	BD29171_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	GYTAGTTCCRATGATGTTAGCTG	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29171_Mstr_Wil2021_COI	ADL-A33	1	BD29171_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	GTAGTGGTAACTACTCATGCTTTA	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29171_Pvir_Dia2013_COI	ADL-A33	1	BD29171_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	CTTAGTGGCATTAATTCGDAATCC	138.5011
BD29171	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76208	WGS84	BD29171	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29171_Rhar_Sim2018_COI	ADL-A33	1	BD29171_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29171	Sample	BD29171	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A33	COI	45	BD29171	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A33	CTGCTGCTATTGCTCATG	138.5011
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29173_Aamu_Bax2006_COI	ADL-A31	1	BD29173_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	GCACAACCGGGATCTTTACTTC	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29173_Pcan_Bot2011_IGS	ADL-A31	1	BD29173_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A31	IGS	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	CGTAATCCTCAGTACTGGCTA	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29173_Pper_Dia2013_COI	ADL-A31	1	BD29173_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	CTTAGTGGCATTAATTCGDAATCC	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29173_Sspa_Oph2007_COI	ADL-A31	1	BD29173_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	GAAGGCCTTCCGATGATC	138.49696
BD29173	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76215	WGS84	BD29173	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29173_Upin_Bot2011_COI	ADL-A31	1	BD29173_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29173	Sample	BD29173	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A31	COI	45	BD29173	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A31	CTTTAATTACAGCGTTTTTATTGTTGT	138.49696
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29174_Cjap_Sim2018_COI	ADL-A30	1	BD29174_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29174_Esin_And2018_Cyb	ADL-A30	1	BD29174_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A30	Cytb	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	ACCCCTCCTCATATCCAACCA	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29174_Mgig_Bot2012_COI	ADL-A30	1	BD29174_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	TCTTATTCGTTGGAGACTTTATAACCCT	138.49501
BD29174	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76254	WGS84	BD29174	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29174_Mjap_Gib2022_COI	ADL-A30	1	BD29174_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29174	Sample	BD29174	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A30	COI	45	BD29174	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A30	AATTTAATTGTGACTAGGCATGGTCTG	138.49501
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.613	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29175_Cmae_Bot2011_COI	ADL-A29	1	BD29175_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	ATGAACAGTCTATCCTCCTTTAG	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29175_Hsan_Wil2021_COI	ADL-A29	1	BD29175_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	CCTGGGCCGGTATAGTAGGT	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29175_Mros_Gib2022_COI	ADL-A29	1	BD29175_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29175_Msal_Bot2012_COI	ADL-A29	1	BD29175_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	GYTAGTTCCRATGATGTTAGCTG	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29175_Mstr_Wil2021_COI	ADL-A29	1	BD29175_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	GTAGTGGTAACTACTCATGCTTTA	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29175_Pvir_Dia2013_COI	ADL-A29	1	BD29175_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	CTTAGTGGCATTAATTCGDAATCC	138.49487
BD29175	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76395	WGS84	BD29175	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29175_Rhar_Sim2018_COI	ADL-A29	1	BD29175_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29175	Sample	BD29175	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A29	COI	45	BD29175	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A29	CTGCTGCTATTGCTCATG	138.49487
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29177_Aamu_Bax2006_COI	ADL-A27	1	BD29177_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	GCACAACCGGGATCTTTACTTC	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29177_Pcan_Bot2011_IGS	ADL-A27	1	BD29177_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A27	IGS	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	CGTAATCCTCAGTACTGGCTA	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29177_Pper_Dia2013_COI	ADL-A27	1	BD29177_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	CTTAGTGGCATTAATTCGDAATCC	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.613	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29177_Sspa_Oph2007_COI	ADL-A27	1	BD29177_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	GAAGGCCTTCCGATGATC	138.4918
BD29177	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76411	WGS84	BD29177	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29177_Upin_Bot2011_COI	ADL-A27	1	BD29177_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29177	Sample	BD29177	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A27	COI	45	BD29177	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A27	CTTTAATTACAGCGTTTTTATTGTTGT	138.4918
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29178_Cjap_Sim2018_COI	ADL-A26	1	BD29178_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29178_Esin_And2018_Cyb	ADL-A26	1	BD29178_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A26	Cytb	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	ACCCCTCCTCATATCCAACCA	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29178_Mgig_Bot2012_COI	ADL-A26	1	BD29178_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	TCTTATTCGTTGGAGACTTTATAACCCT	138.49301
BD29178	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7658	WGS84	BD29178	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29178_Mjap_Gib2022_COI	ADL-A26	1	BD29178_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29178	Sample	BD29178	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A26	COI	45	BD29178	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A26	AATTTAATTGTGACTAGGCATGGTCTG	138.49301
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	34.448	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29179_Cmae_Bot2011_COI	ADL-A25	1	BD29179_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	ATGAACAGTCTATCCTCCTTTAG	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29179_Hsan_Wil2021_COI	ADL-A25	1	BD29179_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	CCTGGGCCGGTATAGTAGGT	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29179_Mros_Gib2022_COI	ADL-A25	1	BD29179_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29179_Msal_Bot2012_COI	ADL-A25	1	BD29179_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	GYTAGTTCCRATGATGTTAGCTG	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29179_Mstr_Wil2021_COI	ADL-A25	1	BD29179_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	GTAGTGGTAACTACTCATGCTTTA	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29179_Pvir_Dia2013_COI	ADL-A25	1	BD29179_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	CTTAGTGGCATTAATTCGDAATCC	138.49085
BD29179	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76572	WGS84	BD29179	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29179_Rhar_Sim2018_COI	ADL-A25	1	BD29179_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29179	Sample	BD29179	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A25	COI	45	BD29179	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A25	CTGCTGCTATTGCTCATG	138.49085
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29180_Aamu_Bax2006_COI	ADL-A24	1	BD29180_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	GCACAACCGGGATCTTTACTTC	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29180_Pcan_Bot2011_IGS	ADL-A24	1	BD29180_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A24	IGS	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	CGTAATCCTCAGTACTGGCTA	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29180_Pper_Dia2013_COI	ADL-A24	1	BD29180_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	CTTAGTGGCATTAATTCGDAATCC	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.921	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29180_Sspa_Oph2007_COI	ADL-A24	1	BD29180_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	GAAGGCCTTCCGATGATC	138.48685
BD29180	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.766	WGS84	BD29180	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29180_Upin_Bot2011_COI	ADL-A24	1	BD29180_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29180	Sample	BD29180	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A24	COI	45	BD29180	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A24	CTTTAATTACAGCGTTTTTATTGTTGT	138.48685
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29181_Cjap_Sim2018_COI	ADL-A23	1	BD29181_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29181_Esin_And2018_Cyb	ADL-A23	1	BD29181_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A23	Cytb	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	ACCCCTCCTCATATCCAACCA	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29181_Mgig_Bot2012_COI	ADL-A23	1	BD29181_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	TCTTATTCGTTGGAGACTTTATAACCCT	138.48983
BD29181	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76741	WGS84	BD29181	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29181_Mjap_Gib2022_COI	ADL-A23	1	BD29181_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29181	Sample	BD29181	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A23	COI	45	BD29181	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A23	AATTTAATTGTGACTAGGCATGGTCTG	138.48983
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29182_Cmae_Bot2011_COI	ADL-A22	1	BD29182_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Cmae_Bot2011_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	ATGAACAGTCTATCCTCCTTTAG	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29182_Hsan_Wil2021_COI	ADL-A22	1	BD29182_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	CCTGGGCCGGTATAGTAGGT	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29182_Mros_Gib2022_COI	ADL-A22	1	BD29182_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29182_Msal_Bot2012_COI	ADL-A22	1	BD29182_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	GYTAGTTCCRATGATGTTAGCTG	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29182_Mstr_Wil2021_COI	ADL-A22	1	BD29182_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	GTAGTGGTAACTACTCATGCTTTA	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29182_Pvir_Dia2013_COI	ADL-A22	1	BD29182_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	CTTAGTGGCATTAATTCGDAATCC	138.48798
BD29182	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76751	WGS84	BD29182	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29182_Rhar_Sim2018_COI	ADL-A22	1	BD29182_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29182	Sample	BD29182	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A22	COI	45	BD29182	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A22	CTGCTGCTATTGCTCATG	138.48798
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29184_Aamu_Bax2006_COI	ADL-A20	1	BD29184_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	GCACAACCGGGATCTTTACTTC	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29184_Pcan_Bot2011_IGS	ADL-A20	1	BD29184_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A20	IGS	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	CGTAATCCTCAGTACTGGCTA	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29184_Pper_Dia2013_COI	ADL-A20	1	BD29184_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	CTTAGTGGCATTAATTCGDAATCC	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.591	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29184_Sspa_Oph2007_COI	ADL-A20	1	BD29184_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	GAAGGCCTTCCGATGATC	138.4887
BD29184	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76872	WGS84	BD29184	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29184_Upin_Bot2011_COI	ADL-A20	1	BD29184_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29184	Sample	BD29184	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A20	COI	45	BD29184	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A20	CTTTAATTACAGCGTTTTTATTGTTGT	138.4887
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29185_Cjap_Sim2018_COI	ADL-A19	1	BD29185_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29185_Esin_And2018_Cyb	ADL-A19	1	BD29185_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A19	Cytb	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	ACCCCTCCTCATATCCAACCA	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29185_Mgig_Bot2012_COI	ADL-A19	1	BD29185_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	TCTTATTCGTTGGAGACTTTATAACCCT	138.48667
BD29185	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76881	WGS84	BD29185	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29185_Mjap_Gib2022_COI	ADL-A19	1	BD29185_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29185	Sample	BD29185	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A19	COI	45	BD29185	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A19	AATTTAATTGTGACTAGGCATGGTCTG	138.48667
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	31.006	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29186_Cmae_Bot2011_COI	ADL-A18	1	BD29186_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	ATGAACAGTCTATCCTCCTTTAG	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29186_Hsan_Wil2021_COI	ADL-A18	1	BD29186_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	CCTGGGCCGGTATAGTAGGT	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29186_Mros_Gib2022_COI	ADL-A18	1	BD29186_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29186_Msal_Bot2012_COI	ADL-A18	1	BD29186_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	GYTAGTTCCRATGATGTTAGCTG	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29186_Mstr_Wil2021_COI	ADL-A18	1	BD29186_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	GTAGTGGTAACTACTCATGCTTTA	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29186_Pvir_Dia2013_COI	ADL-A18	1	BD29186_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	CTTAGTGGCATTAATTCGDAATCC	138.48482
BD29186	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.76912	WGS84	BD29186	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29186_Rhar_Sim2018_COI	ADL-A18	1	BD29186_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29186	Sample	BD29186	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A18	COI	45	BD29186	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A18	CTGCTGCTATTGCTCATG	138.48482
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29188_Aamu_Bax2006_COI	ADL-A16	1	BD29188_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	GCACAACCGGGATCTTTACTTC	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29188_Pcan_Bot2011_IGS	ADL-A16	1	BD29188_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A16	IGS	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	CGTAATCCTCAGTACTGGCTA	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29188_Pper_Dia2013_COI	ADL-A16	1	BD29188_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	CTTAGTGGCATTAATTCGDAATCC	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29188_Sspa_Oph2007_COI	ADL-A16	1	BD29188_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Sspa_Oph2007_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	GAAGGCCTTCCGATGATC	138.48413
BD29188	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7707	WGS84	BD29188	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29188_Upin_Bot2011_COI	ADL-A16	1	BD29188_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29188	Sample	BD29188	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A16	COI	45	BD29188	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A16	CTTTAATTACAGCGTTTTTATTGTTGT	138.48413
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29189_Cjap_Sim2018_COI	ADL-A15	1	BD29189_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29189_Esin_And2018_Cyb	ADL-A15	1	BD29189_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A15	Cytb	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	ACCCCTCCTCATATCCAACCA	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29189_Mgig_Bot2012_COI	ADL-A15	1	BD29189_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	TCTTATTCGTTGGAGACTTTATAACCCT	138.48498
BD29189	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77209	WGS84	BD29189	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29189_Mjap_Gib2022_COI	ADL-A15	1	BD29189_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29189	Sample	BD29189	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A15	COI	45	BD29189	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A15	AATTTAATTGTGACTAGGCATGGTCTG	138.48498
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29191_Aamu_Bax2006_COI	ADL-A13	1	BD29191_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	GCACAACCGGGATCTTTACTTC	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29191_Pcan_Bot2011_IGS	ADL-A13	1	BD29191_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A13	IGS	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	CGTAATCCTCAGTACTGGCTA	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29191_Pper_Dia2013_COI	ADL-A13	1	BD29191_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	CTTAGTGGCATTAATTCGDAATCC	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.6	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29191_Sspa_Oph2007_COI	ADL-A13	1	BD29191_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	GAAGGCCTTCCGATGATC	138.48374
BD29191	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77349	WGS84	BD29191	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29191_Upin_Bot2011_COI	ADL-A13	1	BD29191_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29191	Sample	BD29191	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A13	COI	45	BD29191	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A13	CTTTAATTACAGCGTTTTTATTGTTGT	138.48374
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29192_Cjap_Sim2018_COI	ADL-A12	1	BD29192_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29192_Esin_And2018_Cyb	ADL-A12	1	BD29192_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A12	Cytb	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	ACCCCTCCTCATATCCAACCA	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29192_Mgig_Bot2012_COI	ADL-A12	1	BD29192_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	TCTTATTCGTTGGAGACTTTATAACCCT	138.48197
BD29192	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77372	WGS84	BD29192	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29192_Mjap_Gib2022_COI	ADL-A12	1	BD29192_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29192	Sample	BD29192	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A12	COI	45	BD29192	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A12	AATTTAATTGTGACTAGGCATGGTCTG	138.48197
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	30.949	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29193_Cmae_Bot2011_COI	ADL-A11	1	BD29193_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	ATGAACAGTCTATCCTCCTTTAG	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29193_Hsan_Wil2021_COI	ADL-A11	1	BD29193_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	CCTGGGCCGGTATAGTAGGT	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29193_Mros_Gib2022_COI	ADL-A11	1	BD29193_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29193_Msal_Bot2012_COI	ADL-A11	1	BD29193_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	GYTAGTTCCRATGATGTTAGCTG	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29193_Mstr_Wil2021_COI	ADL-A11	1	BD29193_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	GTAGTGGTAACTACTCATGCTTTA	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29193_Pvir_Dia2013_COI	ADL-A11	1	BD29193_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	CTTAGTGGCATTAATTCGDAATCC	138.48302
BD29193	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77522	WGS84	BD29193	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29193_Rhar_Sim2018_COI	ADL-A11	1	BD29193_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29193	Sample	BD29193	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A11	COI	45	BD29193	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A11	CTGCTGCTATTGCTCATG	138.48302
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29195_Aamu_Bax2006_COI	ADL-A09	1	BD29195_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	GCACAACCGGGATCTTTACTTC	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29195_Pcan_Bot2011_IGS	ADL-A09	1	BD29195_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A09	IGS	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	CGTAATCCTCAGTACTGGCTA	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29195_Pper_Dia2013_COI	ADL-A09	1	BD29195_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	CTTAGTGGCATTAATTCGDAATCC	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.55	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29195_Sspa_Oph2007_COI	ADL-A09	1	BD29195_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	GAAGGCCTTCCGATGATC	138.48218
BD29195	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77669	WGS84	BD29195	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29195_Upin_Bot2011_COI	ADL-A09	1	BD29195_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29195	Sample	BD29195	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A09	COI	45	BD29195	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A09	CTTTAATTACAGCGTTTTTATTGTTGT	138.48218
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29196_Cjap_Sim2018_COI	ADL-A08	1	BD29196_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29196_Esin_And2018_Cyb	ADL-A08	1	BD29196_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A08	Cytb	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	ACCCCTCCTCATATCCAACCA	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29196_Mgig_Bot2012_COI	ADL-A08	1	BD29196_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	TCTTATTCGTTGGAGACTTTATAACCCT	138.47978
BD29196	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77667	WGS84	BD29196	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29196_Mjap_Gib2022_COI	ADL-A08	1	BD29196_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29196	Sample	BD29196	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A08	COI	45	BD29196	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A08	AATTTAATTGTGACTAGGCATGGTCTG	138.47978
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	35.376	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29197_Cmae_Bot2011_COI	ADL-A07	1	BD29197_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	ATGAACAGTCTATCCTCCTTTAG	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29197_Hsan_Wil2021_COI	ADL-A07	1	BD29197_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	CCTGGGCCGGTATAGTAGGT	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29197_Mros_Gib2022_COI	ADL-A07	1	BD29197_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29197_Msal_Bot2012_COI	ADL-A07	1	BD29197_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	GYTAGTTCCRATGATGTTAGCTG	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29197_Mstr_Wil2021_COI	ADL-A07	1	BD29197_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	GTAGTGGTAACTACTCATGCTTTA	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29197_Pvir_Dia2013_COI	ADL-A07	1	BD29197_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	CTTAGTGGCATTAATTCGDAATCC	138.4788
BD29197	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.77825	WGS84	BD29197	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29197_Rhar_Sim2018_COI	ADL-A07	1	BD29197_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29197	Sample	BD29197	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A07	COI	45	BD29197	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A07	CTGCTGCTATTGCTCATG	138.4788
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29199_Aamu_Bax2006_COI	ADL-A05	1	BD29199_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	GCACAACCGGGATCTTTACTTC	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29199_Pcan_Bot2011_IGS	ADL-A05	1	BD29199_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A05	IGS	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	CGTAATCCTCAGTACTGGCTA	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29199_Pper_Dia2013_COI	ADL-A05	1	BD29199_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	CTTAGTGGCATTAATTCGDAATCC	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	37.209	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29199_Sspa_Oph2007_COI	ADL-A05	1	BD29199_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	GAAGGCCTTCCGATGATC	138.47665
BD29199	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78135	WGS84	BD29199	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29199_Upin_Bot2011_COI	ADL-A05	1	BD29199_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29199	Sample	BD29199	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A05	COI	45	BD29199	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A05	CTTTAATTACAGCGTTTTTATTGTTGT	138.47665
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29200_Cjap_Sim2018_COI	ADL-A04	1	BD29200_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Cjap_Sim2018_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29200_Esin_And2018_Cyb	ADL-A04	1	BD29200_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Esin_And2018_Cyb	ADL-A04	Cytb	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	ACCCCTCCTCATATCCAACCA	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29200_Mgig_Bot2012_COI	ADL-A04	1	BD29200_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Mgig_Bot2012_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	TCTTATTCGTTGGAGACTTTATAACCCT	138.47562
BD29200	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78283	WGS84	BD29200	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29200_Mjap_Gib2022_COI	ADL-A04	1	BD29200_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29200	Sample	BD29200	targeted	Absent	Sample	Mjap_Gib2022_COI	ADL-A04	COI	45	BD29200	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A04	AATTTAATTGTGACTAGGCATGGTCTG	138.47562
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	36.645	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29201_Cmae_Bot2011_COI	ADL-A03	1	BD29201_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Present	Sample	Cmae_Bot2011_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	ATGAACAGTCTATCCTCCTTTAG	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29201_Hsan_Wil2021_COI	ADL-A03	1	BD29201_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Hsan_Wil2021_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	CCTGGGCCGGTATAGTAGGT	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29201_Mros_Gib2022_COI	ADL-A03	1	BD29201_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Mros_Gib2022_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29201_Msal_Bot2012_COI	ADL-A03	1	BD29201_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Msal_Bot2012_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	GYTAGTTCCRATGATGTTAGCTG	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29201_Mstr_Wil2021_COI	ADL-A03	1	BD29201_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Mstr_Wil2021_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	GTAGTGGTAACTACTCATGCTTTA	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29201_Pvir_Dia2013_COI	ADL-A03	1	BD29201_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Pvir_Dia2013_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	CTTAGTGGCATTAATTCGDAATCC	138.47409
BD29201	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.7826	WGS84	BD29201	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29201_Rhar_Sim2018_COI	ADL-A03	1	BD29201_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29201	Sample	BD29201	targeted	Absent	Sample	Rhar_Sim2018_COI	ADL-A03	COI	45	BD29201	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A03	CTGCTGCTATTGCTCATG	138.47409
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29203_Aamu_Bax2006_COI	ADL-A01	1	BD29203_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Aamu_Bax2006_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	GCACAACCGGGATCTTTACTTC	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29203_Pcan_Bot2011_IGS	ADL-A01	1	BD29203_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Pcan_Bot2011_IGS	ADL-A01	IGS	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	CGTAATCCTCAGTACTGGCTA	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29203_Pper_Dia2013_COI	ADL-A01	1	BD29203_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Pper_Dia2013_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	CTTAGTGGCATTAATTCGDAATCC	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.614	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29203_Sspa_Oph2007_COI	ADL-A01	1	BD29203_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Present	Sample	Sspa_Oph2007_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	GAAGGCCTTCCGATGATC	138.47237
BD29203	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14411	-34.78433	WGS84	BD29203	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Outer_Harbor_Winter	24/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29203_Upin_Bot2011_COI	ADL-A01	1	BD29203_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29203	Sample	BD29203	targeted	Absent	Sample	Upin_Bot2011_COI	ADL-A01	COI	45	BD29203	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADL-A01	CTTTAATTACAGCGTTTTTATTGTTGT	138.47237
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29209_Cmae_Bot2011_COI	THE-A35	1	BD29209_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	ATGAACAGTCTATCCTCCTTTAG	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29209_Hsan_Wil2021_COI	THE-A35	1	BD29209_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	CCTGGGCCGGTATAGTAGGT	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29209_Mros_Gib2022_COI	THE-A35	1	BD29209_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29209_Msal_Bot2012_COI	THE-A35	1	BD29209_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	GYTAGTTCCRATGATGTTAGCTG	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29209_Mstr_Wil2021_COI	THE-A35	1	BD29209_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	GTAGTGGTAACTACTCATGCTTTA	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29209_Pvir_Dia2013_COI	THE-A35	1	BD29209_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	CTTAGTGGCATTAATTCGDAATCC	133.64129
BD29209	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14581	WGS84	BD29209	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29209_Rhar_Sim2018_COI	THE-A35	1	BD29209_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29209	Sample	BD29209	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A35	COI	45	BD29209	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A35	CTGCTGCTATTGCTCATG	133.64129
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29210_Aamu_Bax2006_COI	THE-A34	1	BD29210_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	GCACAACCGGGATCTTTACTTC	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29210_Pcan_Bot2011_IGS	THE-A34	1	BD29210_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A34	IGS	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	CGTAATCCTCAGTACTGGCTA	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29210_Pper_Dia2013_COI	THE-A34	1	BD29210_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	CTTAGTGGCATTAATTCGDAATCC	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29210_Sspa_Oph2007_COI	THE-A34	1	BD29210_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	GAAGGCCTTCCGATGATC	133.63997
BD29210	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29210	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29210_Upin_Bot2011_COI	THE-A34	1	BD29210_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29210	Sample	BD29210	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A34	COI	45	BD29210	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A34	CTTTAATTACAGCGTTTTTATTGTTGT	133.63997
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29211_Cjap_Sim2018_COI	THE-A33	1	BD29211_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29211_Esin_And2018_Cyb	THE-A33	1	BD29211_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A33	Cytb	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	ACCCCTCCTCATATCCAACCA	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29211_Mgig_Bot2012_COI	THE-A33	1	BD29211_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	TCTTATTCGTTGGAGACTTTATAACCCT	133.63854
BD29211	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14583	WGS84	BD29211	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29211_Mjap_Gib2022_COI	THE-A33	1	BD29211_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29211	Sample	BD29211	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A33	COI	45	BD29211	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A33	AATTTAATTGTGACTAGGCATGGTCTG	133.63854
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29212_Cmae_Bot2011_COI	THE-A32	1	BD29212_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	ATGAACAGTCTATCCTCCTTTAG	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29212_Hsan_Wil2021_COI	THE-A32	1	BD29212_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	CCTGGGCCGGTATAGTAGGT	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29212_Mros_Gib2022_COI	THE-A32	1	BD29212_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29212_Msal_Bot2012_COI	THE-A32	1	BD29212_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	GYTAGTTCCRATGATGTTAGCTG	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29212_Mstr_Wil2021_COI	THE-A32	1	BD29212_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	GTAGTGGTAACTACTCATGCTTTA	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29212_Pvir_Dia2013_COI	THE-A32	1	BD29212_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	CTTAGTGGCATTAATTCGDAATCC	133.63713
BD29212	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14572	WGS84	BD29212	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29212_Rhar_Sim2018_COI	THE-A32	1	BD29212_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29212	Sample	BD29212	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A32	COI	45	BD29212	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A32	CTGCTGCTATTGCTCATG	133.63713
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29214_Aamu_Bax2006_COI	THE-A30	1	BD29214_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	GCACAACCGGGATCTTTACTTC	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29214_Pcan_Bot2011_IGS	THE-A30	1	BD29214_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A30	IGS	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	CGTAATCCTCAGTACTGGCTA	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29214_Pper_Dia2013_COI	THE-A30	1	BD29214_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	CTTAGTGGCATTAATTCGDAATCC	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29214_Sspa_Oph2007_COI	THE-A30	1	BD29214_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	GAAGGCCTTCCGATGATC	133.63639
BD29214	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14691	WGS84	BD29214	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29214_Upin_Bot2011_COI	THE-A30	1	BD29214_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29214	Sample	BD29214	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A30	COI	45	BD29214	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A30	CTTTAATTACAGCGTTTTTATTGTTGT	133.63639
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29215_Cjap_Sim2018_COI	THE-A29	1	BD29215_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29215_Esin_And2018_Cyb	THE-A29	1	BD29215_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A29	Cytb	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	ACCCCTCCTCATATCCAACCA	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29215_Mgig_Bot2012_COI	THE-A29	1	BD29215_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	TCTTATTCGTTGGAGACTTTATAACCCT	133.63786
BD29215	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1469	WGS84	BD29215	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29215_Mjap_Gib2022_COI	THE-A29	1	BD29215_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29215	Sample	BD29215	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A29	COI	45	BD29215	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A29	AATTTAATTGTGACTAGGCATGGTCTG	133.63786
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29216_Cmae_Bot2011_COI	THE-A28	1	BD29216_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	ATGAACAGTCTATCCTCCTTTAG	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29216_Hsan_Wil2021_COI	THE-A28	1	BD29216_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	CCTGGGCCGGTATAGTAGGT	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29216_Mros_Gib2022_COI	THE-A28	1	BD29216_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29216_Msal_Bot2012_COI	THE-A28	1	BD29216_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	GYTAGTTCCRATGATGTTAGCTG	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29216_Mstr_Wil2021_COI	THE-A28	1	BD29216_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	GTAGTGGTAACTACTCATGCTTTA	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29216_Pvir_Dia2013_COI	THE-A28	1	BD29216_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	CTTAGTGGCATTAATTCGDAATCC	133.63866
BD29216	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14673	WGS84	BD29216	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29216_Rhar_Sim2018_COI	THE-A28	1	BD29216_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29216	Sample	BD29216	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A28	COI	45	BD29216	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A28	CTGCTGCTATTGCTCATG	133.63866
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29218_Aamu_Bax2006_COI	THE-A26	1	BD29218_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	GCACAACCGGGATCTTTACTTC	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29218_Pcan_Bot2011_IGS	THE-A26	1	BD29218_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A26	IGS	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	CGTAATCCTCAGTACTGGCTA	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29218_Pper_Dia2013_COI	THE-A26	1	BD29218_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	CTTAGTGGCATTAATTCGDAATCC	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29218_Sspa_Oph2007_COI	THE-A26	1	BD29218_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	GAAGGCCTTCCGATGATC	133.64141
BD29218	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14778	WGS84	BD29218	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29218_Upin_Bot2011_COI	THE-A26	1	BD29218_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29218	Sample	BD29218	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A26	COI	45	BD29218	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A26	CTTTAATTACAGCGTTTTTATTGTTGT	133.64141
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29219_Cjap_Sim2018_COI	THE-A25	1	BD29219_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29219_Esin_And2018_Cyb	THE-A25	1	BD29219_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A25	Cytb	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	ACCCCTCCTCATATCCAACCA	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29219_Mgig_Bot2012_COI	THE-A25	1	BD29219_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	TCTTATTCGTTGGAGACTTTATAACCCT	133.64001
BD29219	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29219	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29219_Mjap_Gib2022_COI	THE-A25	1	BD29219_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29219	Sample	BD29219	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A25	COI	45	BD29219	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A25	AATTTAATTGTGACTAGGCATGGTCTG	133.64001
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29221_Aamu_Bax2006_COI	THE-A23	1	BD29221_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	GCACAACCGGGATCTTTACTTC	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29221_Pcan_Bot2011_IGS	THE-A23	1	BD29221_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A23	IGS	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	CGTAATCCTCAGTACTGGCTA	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29221_Pper_Dia2013_COI	THE-A23	1	BD29221_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	CTTAGTGGCATTAATTCGDAATCC	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29221_Sspa_Oph2007_COI	THE-A23	1	BD29221_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	GAAGGCCTTCCGATGATC	133.63701
BD29221	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14779	WGS84	BD29221	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29221_Upin_Bot2011_COI	THE-A23	1	BD29221_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29221	Sample	BD29221	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A23	COI	45	BD29221	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A23	CTTTAATTACAGCGTTTTTATTGTTGT	133.63701
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29222_Cjap_Sim2018_COI	THE-A22	1	BD29222_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29222_Esin_And2018_Cyb	THE-A22	1	BD29222_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A22	Cytb	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	ACCCCTCCTCATATCCAACCA	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29222_Mgig_Bot2012_COI	THE-A22	1	BD29222_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	TCTTATTCGTTGGAGACTTTATAACCCT	133.63571
BD29222	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14806	WGS84	BD29222	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29222_Mjap_Gib2022_COI	THE-A22	1	BD29222_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29222	Sample	BD29222	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A22	COI	45	BD29222	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A22	AATTTAATTGTGACTAGGCATGGTCTG	133.63571
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29223_Cmae_Bot2011_COI	THE-A21	1	BD29223_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	ATGAACAGTCTATCCTCCTTTAG	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29223_Hsan_Wil2021_COI	THE-A21	1	BD29223_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	CCTGGGCCGGTATAGTAGGT	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29223_Mros_Gib2022_COI	THE-A21	1	BD29223_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29223_Msal_Bot2012_COI	THE-A21	1	BD29223_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	GYTAGTTCCRATGATGTTAGCTG	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29223_Mstr_Wil2021_COI	THE-A21	1	BD29223_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	GTAGTGGTAACTACTCATGCTTTA	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29223_Pvir_Dia2013_COI	THE-A21	1	BD29223_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	CTTAGTGGCATTAATTCGDAATCC	133.63495
BD29223	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14892	WGS84	BD29223	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29223_Rhar_Sim2018_COI	THE-A21	1	BD29223_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29223	Sample	BD29223	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A21	COI	45	BD29223	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A21	CTGCTGCTATTGCTCATG	133.63495
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29225_Aamu_Bax2006_COI	THE-A19	1	BD29225_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	GCACAACCGGGATCTTTACTTC	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29225_Pcan_Bot2011_IGS	THE-A19	1	BD29225_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A19	IGS	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	CGTAATCCTCAGTACTGGCTA	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29225_Pper_Dia2013_COI	THE-A19	1	BD29225_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	CTTAGTGGCATTAATTCGDAATCC	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29225_Sspa_Oph2007_COI	THE-A19	1	BD29225_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	GAAGGCCTTCCGATGATC	133.63764
BD29225	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.14905	WGS84	BD29225	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29225_Upin_Bot2011_COI	THE-A19	1	BD29225_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29225	Sample	BD29225	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A19	COI	45	BD29225	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A19	CTTTAATTACAGCGTTTTTATTGTTGT	133.63764
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29226_Cjap_Sim2018_COI	THE-A18	1	BD29226_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29226_Esin_And2018_Cyb	THE-A18	1	BD29226_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A18	Cytb	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	ACCCCTCCTCATATCCAACCA	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29226_Mgig_Bot2012_COI	THE-A18	1	BD29226_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	TCTTATTCGTTGGAGACTTTATAACCCT	133.6378
BD29226	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15345	WGS84	BD29226	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29226_Mjap_Gib2022_COI	THE-A18	1	BD29226_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29226	Sample	BD29226	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A18	COI	45	BD29226	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A18	AATTTAATTGTGACTAGGCATGGTCTG	133.6378
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29227_Cmae_Bot2011_COI	THE-A17	1	BD29227_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	ATGAACAGTCTATCCTCCTTTAG	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29227_Hsan_Wil2021_COI	THE-A17	1	BD29227_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	CCTGGGCCGGTATAGTAGGT	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29227_Mros_Gib2022_COI	THE-A17	1	BD29227_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29227_Msal_Bot2012_COI	THE-A17	1	BD29227_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	GYTAGTTCCRATGATGTTAGCTG	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29227_Mstr_Wil2021_COI	THE-A17	1	BD29227_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	GTAGTGGTAACTACTCATGCTTTA	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29227_Pvir_Dia2013_COI	THE-A17	1	BD29227_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	CTTAGTGGCATTAATTCGDAATCC	133.63938
BD29227	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15351	WGS84	BD29227	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29227_Rhar_Sim2018_COI	THE-A17	1	BD29227_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29227	Sample	BD29227	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A17	COI	45	BD29227	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A17	CTGCTGCTATTGCTCATG	133.63938
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29229_Aamu_Bax2006_COI	THE-A15	1	BD29229_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	GCACAACCGGGATCTTTACTTC	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29229_Pcan_Bot2011_IGS	THE-A15	1	BD29229_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A15	IGS	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	CGTAATCCTCAGTACTGGCTA	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29229_Pper_Dia2013_COI	THE-A15	1	BD29229_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	CTTAGTGGCATTAATTCGDAATCC	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29229_Sspa_Oph2007_COI	THE-A15	1	BD29229_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	GAAGGCCTTCCGATGATC	133.63994
BD29229	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15236	WGS84	BD29229	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29229_Upin_Bot2011_COI	THE-A15	1	BD29229_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29229	Sample	BD29229	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A15	COI	45	BD29229	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A15	CTTTAATTACAGCGTTTTTATTGTTGT	133.63994
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29230_Cmae_Bot2011_COI	THE-A14	1	BD29230_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	ATGAACAGTCTATCCTCCTTTAG	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29230_Hsan_Wil2021_COI	THE-A14	1	BD29230_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	CCTGGGCCGGTATAGTAGGT	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29230_Mros_Gib2022_COI	THE-A14	1	BD29230_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29230_Msal_Bot2012_COI	THE-A14	1	BD29230_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	GYTAGTTCCRATGATGTTAGCTG	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29230_Mstr_Wil2021_COI	THE-A14	1	BD29230_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	GTAGTGGTAACTACTCATGCTTTA	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29230_Pvir_Dia2013_COI	THE-A14	1	BD29230_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	CTTAGTGGCATTAATTCGDAATCC	133.63852
BD29230	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15235	WGS84	BD29230	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29230_Rhar_Sim2018_COI	THE-A14	1	BD29230_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29230	Sample	BD29230	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A14	COI	45	BD29230	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A14	CTGCTGCTATTGCTCATG	133.63852
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29232_Aamu_Bax2006_COI	THE-A11	1	BD29232_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	GCACAACCGGGATCTTTACTTC	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29232_Pcan_Bot2011_IGS	THE-A11	1	BD29232_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A11	IGS	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	CGTAATCCTCAGTACTGGCTA	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29232_Pper_Dia2013_COI	THE-A11	1	BD29232_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	CTTAGTGGCATTAATTCGDAATCC	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29232_Sspa_Oph2007_COI	THE-A11	1	BD29232_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	GAAGGCCTTCCGATGATC	133.6349
BD29232	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15124	WGS84	BD29232	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29232_Upin_Bot2011_COI	THE-A11	1	BD29232_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29232	Sample	BD29232	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A11	COI	45	BD29232	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A11	CTTTAATTACAGCGTTTTTATTGTTGT	133.6349
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29233_Cjap_Sim2018_COI	THE-A12	1	BD29233_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29233_Esin_And2018_Cyb	THE-A12	1	BD29233_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A12	Cytb	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	ACCCCTCCTCATATCCAACCA	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29233_Mgig_Bot2012_COI	THE-A12	1	BD29233_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	TCTTATTCGTTGGAGACTTTATAACCCT	133.63562
BD29233	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15226	WGS84	BD29233	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29233_Mjap_Gib2022_COI	THE-A12	1	BD29233_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29233	Sample	BD29233	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A12	COI	45	BD29233	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A12	AATTTAATTGTGACTAGGCATGGTCTG	133.63562
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29234_Cmae_Bot2011_COI	THE-A10	1	BD29234_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	ATGAACAGTCTATCCTCCTTTAG	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29234_Hsan_Wil2021_COI	THE-A10	1	BD29234_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	CCTGGGCCGGTATAGTAGGT	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29234_Mros_Gib2022_COI	THE-A10	1	BD29234_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29234_Msal_Bot2012_COI	THE-A10	1	BD29234_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	GYTAGTTCCRATGATGTTAGCTG	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29234_Mstr_Wil2021_COI	THE-A10	1	BD29234_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	GTAGTGGTAACTACTCATGCTTTA	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29234_Pvir_Dia2013_COI	THE-A10	1	BD29234_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	CTTAGTGGCATTAATTCGDAATCC	133.63622
BD29234	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15136	WGS84	BD29234	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29234_Rhar_Sim2018_COI	THE-A10	1	BD29234_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29234	Sample	BD29234	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A10	COI	45	BD29234	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A10	CTGCTGCTATTGCTCATG	133.63622
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29236_Aamu_Bax2006_COI	THE-A08	1	BD29236_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	GCACAACCGGGATCTTTACTTC	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29236_Pcan_Bot2011_IGS	THE-A08	1	BD29236_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A08	IGS	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	CGTAATCCTCAGTACTGGCTA	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29236_Pper_Dia2013_COI	THE-A08	1	BD29236_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	CTTAGTGGCATTAATTCGDAATCC	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29236_Sspa_Oph2007_COI	THE-A08	1	BD29236_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	GAAGGCCTTCCGATGATC	133.63907
BD29236	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15137	WGS84	BD29236	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29236_Upin_Bot2011_COI	THE-A08	1	BD29236_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29236	Sample	BD29236	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A08	COI	45	BD29236	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A08	CTTTAATTACAGCGTTTTTATTGTTGT	133.63907
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29237_Cjap_Sim2018_COI	THE-A07	1	BD29237_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29237_Esin_And2018_Cyb	THE-A07	1	BD29237_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A07	Cytb	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	ACCCCTCCTCATATCCAACCA	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29237_Mgig_Bot2012_COI	THE-A07	1	BD29237_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	TCTTATTCGTTGGAGACTTTATAACCCT	133.64068
BD29237	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15138	WGS84	BD29237	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29237_Mjap_Gib2022_COI	THE-A07	1	BD29237_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29237	Sample	BD29237	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A07	COI	45	BD29237	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A07	AATTTAATTGTGACTAGGCATGGTCTG	133.64068
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29238_Cmae_Bot2011_COI	THE-A06	1	BD29238_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	ATGAACAGTCTATCCTCCTTTAG	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29238_Hsan_Wil2021_COI	THE-A06	1	BD29238_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	CCTGGGCCGGTATAGTAGGT	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29238_Mros_Gib2022_COI	THE-A06	1	BD29238_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29238_Msal_Bot2012_COI	THE-A06	1	BD29238_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	GYTAGTTCCRATGATGTTAGCTG	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29238_Mstr_Wil2021_COI	THE-A06	1	BD29238_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	GTAGTGGTAACTACTCATGCTTTA	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29238_Pvir_Dia2013_COI	THE-A06	1	BD29238_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	CTTAGTGGCATTAATTCGDAATCC	133.64205
BD29238	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15145	WGS84	BD29238	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29238_Rhar_Sim2018_COI	THE-A06	1	BD29238_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29238	Sample	BD29238	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A06	COI	45	BD29238	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A06	CTGCTGCTATTGCTCATG	133.64205
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29240_Cjap_Sim2018_COI	THE-A04	1	BD29240_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Cjap_Sim2018_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	TTAATATACGGTCATTTGGTATGAGTATAGATC	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29240_Esin_And2018_Cyb	THE-A04	1	BD29240_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Esin_And2018_Cyb	THE-A04	Cytb	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	ACCCCTCCTCATATCCAACCA	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29240_Mgig_Bot2012_COI	THE-A04	1	BD29240_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Mgig_Bot2012_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	TCTTATTCGTTGGAGACTTTATAACCCT	133.63986
BD29240	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.1504	WGS84	BD29240	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29240_Mjap_Gib2022_COI	THE-A04	1	BD29240_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29240	Sample	BD29240	targeted	Absent	Sample	Mjap_Gib2022_COI	THE-A04	COI	45	BD29240	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A04	AATTTAATTGTGACTAGGCATGGTCTG	133.63986
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29241_Cmae_Bot2011_COI	THE-A03	1	BD29241_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Cmae_Bot2011_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	ATGAACAGTCTATCCTCCTTTAG	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29241_Hsan_Wil2021_COI	THE-A03	1	BD29241_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Hsan_Wil2021_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	CCTGGGCCGGTATAGTAGGT	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29241_Mros_Gib2022_COI	THE-A03	1	BD29241_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Mros_Gib2022_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	ACTTCTACCTCCTGCTCTTCTACTTCTC	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29241_Msal_Bot2012_COI	THE-A03	1	BD29241_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Msal_Bot2012_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	GYTAGTTCCRATGATGTTAGCTG	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29241_Mstr_Wil2021_COI	THE-A03	1	BD29241_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Mstr_Wil2021_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	GTAGTGGTAACTACTCATGCTTTA	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29241_Pvir_Dia2013_COI	THE-A03	1	BD29241_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Pvir_Dia2013_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	CTTAGTGGCATTAATTCGDAATCC	133.63825
BD29241	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15048	WGS84	BD29241	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29241_Rhar_Sim2018_COI	THE-A03	1	BD29241_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29241	Sample	BD29241	targeted	Absent	Sample	Rhar_Sim2018_COI	THE-A03	COI	45	BD29241	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A03	CTGCTGCTATTGCTCATG	133.63825
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29243_Aamu_Bax2006_COI	THE-A01	1	BD29243_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Aamu_Bax2006_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	GCACAACCGGGATCTTTACTTC	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29243_Pcan_Bot2011_IGS	THE-A01	1	BD29243_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Pcan_Bot2011_IGS	THE-A01	IGS	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	CGTAATCCTCAGTACTGGCTA	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29243_Pper_Dia2013_COI	THE-A01	1	BD29243_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Pper_Dia2013_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	CTTAGTGGCATTAATTCGDAATCC	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29243_Sspa_Oph2007_COI	THE-A01	1	BD29243_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Sspa_Oph2007_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	GAAGGCCTTCCGATGATC	133.63322
BD29243	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-32.15022	WGS84	BD29243	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Thevenard_Winter	22/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29243_Upin_Bot2011_COI	THE-A01	1	BD29243_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29243	Sample	BD29243	targeted	Absent	Sample	Upin_Bot2011_COI	THE-A01	COI	45	BD29243	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	THE-A01	CTTTAATTACAGCGTTTTTATTGTTGT	133.63322
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29244_Cjap_Sim2018_COI	PLO-A40	1	BD29244_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29244_Esin_And2018_Cyb	PLO-A40	1	BD29244_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A40	Cytb	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	ACCCCTCCTCATATCCAACCA	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29244_Mgig_Bot2012_COI	PLO-A40	1	BD29244_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	TCTTATTCGTTGGAGACTTTATAACCCT	135.87311
BD29244	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.73994	WGS84	BD29244	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29244_Mjap_Gib2022_COI	PLO-A40	1	BD29244_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29244	Sample	BD29244	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A40	COI	45	BD29244	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A40	AATTTAATTGTGACTAGGCATGGTCTG	135.87311
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29245_Cmae_Bot2011_COI	PLO-A39	1	BD29245_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	ATGAACAGTCTATCCTCCTTTAG	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29245_Hsan_Wil2021_COI	PLO-A39	1	BD29245_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	CCTGGGCCGGTATAGTAGGT	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29245_Mros_Gib2022_COI	PLO-A39	1	BD29245_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29245_Msal_Bot2012_COI	PLO-A39	1	BD29245_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	GYTAGTTCCRATGATGTTAGCTG	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29245_Mstr_Wil2021_COI	PLO-A39	1	BD29245_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	GTAGTGGTAACTACTCATGCTTTA	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29245_Pvir_Dia2013_COI	PLO-A39	1	BD29245_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	CTTAGTGGCATTAATTCGDAATCC	135.8719
BD29245	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74189	WGS84	BD29245	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29245_Rhar_Sim2018_COI	PLO-A39	1	BD29245_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29245	Sample	BD29245	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A39	COI	45	BD29245	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A39	CTGCTGCTATTGCTCATG	135.8719
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29247_Aamu_Bax2006_COI	PLO-A37	1	BD29247_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	GCACAACCGGGATCTTTACTTC	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29247_Pcan_Bot2011_IGS	PLO-A37	1	BD29247_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A37	IGS	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	CGTAATCCTCAGTACTGGCTA	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29247_Pper_Dia2013_COI	PLO-A37	1	BD29247_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	CTTAGTGGCATTAATTCGDAATCC	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.462	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29247_Sspa_Oph2007_COI	PLO-A37	1	BD29247_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	GAAGGCCTTCCGATGATC	135.87481
BD29247	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74229	WGS84	BD29247	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29247_Upin_Bot2011_COI	PLO-A37	1	BD29247_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29247	Sample	BD29247	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A37	COI	45	BD29247	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A37	CTTTAATTACAGCGTTTTTATTGTTGT	135.87481
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29248_Cjap_Sim2018_COI	PLO-A36	1	BD29248_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29248_Esin_And2018_Cyb	PLO-A36	1	BD29248_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A36	Cytb	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	ACCCCTCCTCATATCCAACCA	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29248_Mgig_Bot2012_COI	PLO-A36	1	BD29248_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	TCTTATTCGTTGGAGACTTTATAACCCT	135.87696
BD29248	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.74192	WGS84	BD29248	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29248_Mjap_Gib2022_COI	PLO-A36	1	BD29248_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29248	Sample	BD29248	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A36	COI	45	BD29248	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A36	AATTTAATTGTGACTAGGCATGGTCTG	135.87696
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29249_Cmae_Bot2011_COI	PLO-A35	1	BD29249_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	ATGAACAGTCTATCCTCCTTTAG	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29249_Hsan_Wil2021_COI	PLO-A35	1	BD29249_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	CCTGGGCCGGTATAGTAGGT	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29249_Mros_Gib2022_COI	PLO-A35	1	BD29249_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29249_Msal_Bot2012_COI	PLO-A35	1	BD29249_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	GYTAGTTCCRATGATGTTAGCTG	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29249_Mstr_Wil2021_COI	PLO-A35	1	BD29249_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	GTAGTGGTAACTACTCATGCTTTA	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29249_Pvir_Dia2013_COI	PLO-A35	1	BD29249_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	CTTAGTGGCATTAATTCGDAATCC	135.87281
BD29249	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71622	WGS84	BD29249	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29249_Rhar_Sim2018_COI	PLO-A35	1	BD29249_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29249	Sample	BD29249	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A35	COI	45	BD29249	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A35	CTGCTGCTATTGCTCATG	135.87281
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29250_Aamu_Bax2006_COI	PLO-A34	1	BD29250_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	GCACAACCGGGATCTTTACTTC	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29250_Pcan_Bot2011_IGS	PLO-A34	1	BD29250_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A34	IGS	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	CGTAATCCTCAGTACTGGCTA	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29250_Pper_Dia2013_COI	PLO-A34	1	BD29250_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	CTTAGTGGCATTAATTCGDAATCC	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	39.573	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29250_Sspa_Oph2007_COI	PLO-A34	1	BD29250_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	GAAGGCCTTCCGATGATC	135.8746
BD29250	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71647	WGS84	BD29250	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29250_Upin_Bot2011_COI	PLO-A34	1	BD29250_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29250	Sample	BD29250	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A34	COI	45	BD29250	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A34	CTTTAATTACAGCGTTTTTATTGTTGT	135.8746
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29251_Cjap_Sim2018_COI	PLO-A33	1	BD29251_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29251_Esin_And2018_Cyb	PLO-A33	1	BD29251_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A33	Cytb	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	ACCCCTCCTCATATCCAACCA	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29251_Mgig_Bot2012_COI	PLO-A33	1	BD29251_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	TCTTATTCGTTGGAGACTTTATAACCCT	135.87175
BD29251	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71465	WGS84	BD29251	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29251_Mjap_Gib2022_COI	PLO-A33	1	BD29251_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29251	Sample	BD29251	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A33	COI	45	BD29251	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A33	AATTTAATTGTGACTAGGCATGGTCTG	135.87175
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29252_Cmae_Bot2011_COI	PLO-A32	1	BD29252_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	ATGAACAGTCTATCCTCCTTTAG	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29252_Hsan_Wil2021_COI	PLO-A32	1	BD29252_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	CCTGGGCCGGTATAGTAGGT	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29252_Mros_Gib2022_COI	PLO-A32	1	BD29252_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29252_Msal_Bot2012_COI	PLO-A32	1	BD29252_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	GYTAGTTCCRATGATGTTAGCTG	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29252_Mstr_Wil2021_COI	PLO-A32	1	BD29252_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	GTAGTGGTAACTACTCATGCTTTA	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29252_Pvir_Dia2013_COI	PLO-A32	1	BD29252_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	CTTAGTGGCATTAATTCGDAATCC	135.87354
BD29252	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71476	WGS84	BD29252	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29252_Rhar_Sim2018_COI	PLO-A32	1	BD29252_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29252	Sample	BD29252	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A32	COI	45	BD29252	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A32	CTGCTGCTATTGCTCATG	135.87354
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29254_Aamu_Bax2006_COI	PLO-A30	1	BD29254_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	GCACAACCGGGATCTTTACTTC	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29254_Pcan_Bot2011_IGS	PLO-A30	1	BD29254_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A30	IGS	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	CGTAATCCTCAGTACTGGCTA	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29254_Pper_Dia2013_COI	PLO-A30	1	BD29254_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	CTTAGTGGCATTAATTCGDAATCC	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.412	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29254_Sspa_Oph2007_COI	PLO-A30	1	BD29254_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	GAAGGCCTTCCGATGATC	135.87692
BD29254	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71628	WGS84	BD29254	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29254_Upin_Bot2011_COI	PLO-A30	1	BD29254_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29254	Sample	BD29254	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A30	COI	45	BD29254	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A30	CTTTAATTACAGCGTTTTTATTGTTGT	135.87692
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29255_Cjap_Sim2018_COI	PLO-A29	1	BD29255_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29255_Esin_And2018_Cyb	PLO-A29	1	BD29255_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A29	Cytb	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	ACCCCTCCTCATATCCAACCA	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29255_Mgig_Bot2012_COI	PLO-A29	1	BD29255_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	TCTTATTCGTTGGAGACTTTATAACCCT	135.87799
BD29255	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71472	WGS84	BD29255	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29255_Mjap_Gib2022_COI	PLO-A29	1	BD29255_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29255	Sample	BD29255	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A29	COI	45	BD29255	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A29	AATTTAATTGTGACTAGGCATGGTCTG	135.87799
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29256_Cmae_Bot2011_COI	PLO-A28	1	BD29256_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	ATGAACAGTCTATCCTCCTTTAG	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29256_Hsan_Wil2021_COI	PLO-A28	1	BD29256_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	CCTGGGCCGGTATAGTAGGT	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29256_Mros_Gib2022_COI	PLO-A28	1	BD29256_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29256_Msal_Bot2012_COI	PLO-A28	1	BD29256_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	GYTAGTTCCRATGATGTTAGCTG	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29256_Mstr_Wil2021_COI	PLO-A28	1	BD29256_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	GTAGTGGTAACTACTCATGCTTTA	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29256_Pvir_Dia2013_COI	PLO-A28	1	BD29256_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	CTTAGTGGCATTAATTCGDAATCC	135.87249
BD29256	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71316	WGS84	BD29256	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29256_Rhar_Sim2018_COI	PLO-A28	1	BD29256_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29256	Sample	BD29256	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A28	COI	45	BD29256	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A28	CTGCTGCTATTGCTCATG	135.87249
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29258_Aamu_Bax2006_COI	PLO-A26	1	BD29258_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	GCACAACCGGGATCTTTACTTC	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29258_Pcan_Bot2011_IGS	PLO-A26	1	BD29258_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A26	IGS	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	CGTAATCCTCAGTACTGGCTA	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29258_Pper_Dia2013_COI	PLO-A26	1	BD29258_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	CTTAGTGGCATTAATTCGDAATCC	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.218	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29258_Sspa_Oph2007_COI	PLO-A26	1	BD29258_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	GAAGGCCTTCCGATGATC	135.87367
BD29258	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71143	WGS84	BD29258	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29258_Upin_Bot2011_COI	PLO-A26	1	BD29258_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29258	Sample	BD29258	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A26	COI	45	BD29258	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A26	CTTTAATTACAGCGTTTTTATTGTTGT	135.87367
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29259_Cjap_Sim2018_COI	PLO-A25	1	BD29259_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29259_Esin_And2018_Cyb	PLO-A25	1	BD29259_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A25	Cytb	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	ACCCCTCCTCATATCCAACCA	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29259_Mgig_Bot2012_COI	PLO-A25	1	BD29259_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	TCTTATTCGTTGGAGACTTTATAACCCT	135.87256
BD29259	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70989	WGS84	BD29259	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29259_Mjap_Gib2022_COI	PLO-A25	1	BD29259_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29259	Sample	BD29259	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A25	COI	45	BD29259	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A25	AATTTAATTGTGACTAGGCATGGTCTG	135.87256
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29261_Aamu_Bax2006_COI	PLO-A24	1	BD29261_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	GCACAACCGGGATCTTTACTTC	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29261_Pcan_Bot2011_IGS	PLO-A24	1	BD29261_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A24	IGS	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	CGTAATCCTCAGTACTGGCTA	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29261_Pper_Dia2013_COI	PLO-A24	1	BD29261_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	CTTAGTGGCATTAATTCGDAATCC	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.05	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29261_Sspa_Oph2007_COI	PLO-A24	1	BD29261_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	GAAGGCCTTCCGATGATC	135.87139
BD29261	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29261	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29261_Upin_Bot2011_COI	PLO-A24	1	BD29261_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29261	Sample	BD29261	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A24	COI	45	BD29261	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A24	CTTTAATTACAGCGTTTTTATTGTTGT	135.87139
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29262_Cjap_Sim2018_COI	PLO-A23	1	BD29262_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29262_Esin_And2018_Cyb	PLO-A23	1	BD29262_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A23	Cytb	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	ACCCCTCCTCATATCCAACCA	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29262_Mgig_Bot2012_COI	PLO-A23	1	BD29262_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	TCTTATTCGTTGGAGACTTTATAACCCT	135.87022
BD29262	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.70995	WGS84	BD29262	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29262_Mjap_Gib2022_COI	PLO-A23	1	BD29262_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29262	Sample	BD29262	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A23	COI	45	BD29262	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A23	AATTTAATTGTGACTAGGCATGGTCTG	135.87022
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29265_Aamu_Bax2006_COI	PLO-A20	1	BD29265_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	GCACAACCGGGATCTTTACTTC	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29265_Pcan_Bot2011_IGS	PLO-A20	1	BD29265_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A20	IGS	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	CGTAATCCTCAGTACTGGCTA	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29265_Pper_Dia2013_COI	PLO-A20	1	BD29265_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	CTTAGTGGCATTAATTCGDAATCC	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	41.913	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29265_Sspa_Oph2007_COI	PLO-A20	1	BD29265_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	GAAGGCCTTCCGATGATC	135.86686
BD29265	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71153	WGS84	BD29265	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29265_Upin_Bot2011_COI	PLO-A20	1	BD29265_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29265	Sample	BD29265	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A20	COI	45	BD29265	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A20	CTTTAATTACAGCGTTTTTATTGTTGT	135.86686
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29266_Cjap_Sim2018_COI	PLO-A19	1	BD29266_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29266_Esin_And2018_Cyb	PLO-A19	1	BD29266_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A19	Cytb	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	ACCCCTCCTCATATCCAACCA	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29266_Mgig_Bot2012_COI	PLO-A19	1	BD29266_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	TCTTATTCGTTGGAGACTTTATAACCCT	135.86574
BD29266	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71321	WGS84	BD29266	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29266_Mjap_Gib2022_COI	PLO-A19	1	BD29266_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29266	Sample	BD29266	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A19	COI	45	BD29266	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A19	AATTTAATTGTGACTAGGCATGGTCTG	135.86574
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29267_Cmae_Bot2011_COI	PLO-A18	1	BD29267_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	ATGAACAGTCTATCCTCCTTTAG	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29267_Hsan_Wil2021_COI	PLO-A18	1	BD29267_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	CCTGGGCCGGTATAGTAGGT	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29267_Mros_Gib2022_COI	PLO-A18	1	BD29267_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29267_Msal_Bot2012_COI	PLO-A18	1	BD29267_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	GYTAGTTCCRATGATGTTAGCTG	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29267_Mstr_Wil2021_COI	PLO-A18	1	BD29267_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	GTAGTGGTAACTACTCATGCTTTA	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29267_Pvir_Dia2013_COI	PLO-A18	1	BD29267_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	CTTAGTGGCATTAATTCGDAATCC	135.86819
BD29267	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71309	WGS84	BD29267	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29267_Rhar_Sim2018_COI	PLO-A18	1	BD29267_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29267	Sample	BD29267	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A18	COI	45	BD29267	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A18	CTGCTGCTATTGCTCATG	135.86819
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29269_Aamu_Bax2006_COI	PLO-A16	1	BD29269_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	GCACAACCGGGATCTTTACTTC	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29269_Pcan_Bot2011_IGS	PLO-A16	1	BD29269_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A16	IGS	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	CGTAATCCTCAGTACTGGCTA	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29269_Pper_Dia2013_COI	PLO-A16	1	BD29269_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	CTTAGTGGCATTAATTCGDAATCC	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29269_Sspa_Oph2007_COI	PLO-A16	1	BD29269_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Sspa_Oph2007_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	GAAGGCCTTCCGATGATC	135.86685
BD29269	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71482	WGS84	BD29269	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29269_Upin_Bot2011_COI	PLO-A16	1	BD29269_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29269	Sample	BD29269	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A16	COI	45	BD29269	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A16	CTTTAATTACAGCGTTTTTATTGTTGT	135.86685
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29270_Cmae_Bot2011_COI	PLO-A15	1	BD29270_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	ATGAACAGTCTATCCTCCTTTAG	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29270_Hsan_Wil2021_COI	PLO-A15	1	BD29270_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	CCTGGGCCGGTATAGTAGGT	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29270_Mros_Gib2022_COI	PLO-A15	1	BD29270_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29270_Msal_Bot2012_COI	PLO-A15	1	BD29270_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	GYTAGTTCCRATGATGTTAGCTG	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29270_Mstr_Wil2021_COI	PLO-A15	1	BD29270_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	GTAGTGGTAACTACTCATGCTTTA	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29270_Pvir_Dia2013_COI	PLO-A15	1	BD29270_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	CTTAGTGGCATTAATTCGDAATCC	135.86365
BD29270	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29270	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29270_Rhar_Sim2018_COI	PLO-A15	1	BD29270_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29270	Sample	BD29270	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A15	COI	45	BD29270	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A15	CTGCTGCTATTGCTCATG	135.86365
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29272_Aamu_Bax2006_COI	PLO-A13	1	BD29272_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	GCACAACCGGGATCTTTACTTC	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29272_Pcan_Bot2011_IGS	PLO-A13	1	BD29272_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A13	IGS	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	CGTAATCCTCAGTACTGGCTA	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29272_Pper_Dia2013_COI	PLO-A13	1	BD29272_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	CTTAGTGGCATTAATTCGDAATCC	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.94	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29272_Sspa_Oph2007_COI	PLO-A13	1	BD29272_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	GAAGGCCTTCCGATGATC	135.86812
BD29272	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71634	WGS84	BD29272	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29272_Upin_Bot2011_COI	PLO-A13	1	BD29272_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29272	Sample	BD29272	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A13	COI	45	BD29272	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A13	CTTTAATTACAGCGTTTTTATTGTTGT	135.86812
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29273_Cjap_Sim2018_COI	PLO-A12	1	BD29273_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29273_Esin_And2018_Cyb	PLO-A12	1	BD29273_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A12	Cytb	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	ACCCCTCCTCATATCCAACCA	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29273_Mgig_Bot2012_COI	PLO-A12	1	BD29273_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	TCTTATTCGTTGGAGACTTTATAACCCT	135.86717
BD29273	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7179	WGS84	BD29273	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29273_Mjap_Gib2022_COI	PLO-A12	1	BD29273_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29273	Sample	BD29273	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A12	COI	45	BD29273	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A12	AATTTAATTGTGACTAGGCATGGTCTG	135.86717
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29274_Cmae_Bot2011_COI	PLO-A11	1	BD29274_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	ATGAACAGTCTATCCTCCTTTAG	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29274_Hsan_Wil2021_COI	PLO-A11	1	BD29274_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	CCTGGGCCGGTATAGTAGGT	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29274_Mros_Gib2022_COI	PLO-A11	1	BD29274_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29274_Msal_Bot2012_COI	PLO-A11	1	BD29274_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	GYTAGTTCCRATGATGTTAGCTG	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29274_Mstr_Wil2021_COI	PLO-A11	1	BD29274_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	GTAGTGGTAACTACTCATGCTTTA	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29274_Pvir_Dia2013_COI	PLO-A11	1	BD29274_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	CTTAGTGGCATTAATTCGDAATCC	135.86498
BD29274	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71792	WGS84	BD29274	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29274_Rhar_Sim2018_COI	PLO-A11	1	BD29274_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29274	Sample	BD29274	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A11	COI	45	BD29274	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A11	CTGCTGCTATTGCTCATG	135.86498
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29276_Aamu_Bax2006_COI	PLO-A09	1	BD29276_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	GCACAACCGGGATCTTTACTTC	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29276_Pcan_Bot2011_IGS	PLO-A09	1	BD29276_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A09	IGS	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	CGTAATCCTCAGTACTGGCTA	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29276_Pper_Dia2013_COI	PLO-A09	1	BD29276_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	CTTAGTGGCATTAATTCGDAATCC	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	33.488	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29276_Sspa_Oph2007_COI	PLO-A09	1	BD29276_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	GAAGGCCTTCCGATGATC	135.87123
BD29276	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71712	WGS84	BD29276	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29276_Upin_Bot2011_COI	PLO-A09	1	BD29276_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29276	Sample	BD29276	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A09	COI	45	BD29276	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A09	CTTTAATTACAGCGTTTTTATTGTTGT	135.87123
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29277_Cjap_Sim2018_COI	PLO-A08	1	BD29277_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29277_Esin_And2018_Cyb	PLO-A08	1	BD29277_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A08	Cytb	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	ACCCCTCCTCATATCCAACCA	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29277_Mgig_Bot2012_COI	PLO-A08	1	BD29277_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	TCTTATTCGTTGGAGACTTTATAACCCT	135.87218
BD29277	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71852	WGS84	BD29277	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29277_Mjap_Gib2022_COI	PLO-A08	1	BD29277_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29277	Sample	BD29277	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A08	COI	45	BD29277	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A08	AATTTAATTGTGACTAGGCATGGTCTG	135.87218
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29278_Cmae_Bot2011_COI	PLO-A07	1	BD29278_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	ATGAACAGTCTATCCTCCTTTAG	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29278_Hsan_Wil2021_COI	PLO-A07	1	BD29278_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	CCTGGGCCGGTATAGTAGGT	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29278_Mros_Gib2022_COI	PLO-A07	1	BD29278_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29278_Msal_Bot2012_COI	PLO-A07	1	BD29278_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	GYTAGTTCCRATGATGTTAGCTG	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29278_Mstr_Wil2021_COI	PLO-A07	1	BD29278_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	GTAGTGGTAACTACTCATGCTTTA	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29278_Pvir_Dia2013_COI	PLO-A07	1	BD29278_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	CTTAGTGGCATTAATTCGDAATCC	135.8729
BD29278	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71939	WGS84	BD29278	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29278_Rhar_Sim2018_COI	PLO-A07	1	BD29278_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29278	Sample	BD29278	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A07	COI	45	BD29278	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A07	CTGCTGCTATTGCTCATG	135.8729
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29280_Cjap_Sim2018_COI	PLO-A05	1	BD29280_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29280_Esin_And2018_Cyb	PLO-A05	1	BD29280_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A05	Cytb	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	ACCCCTCCTCATATCCAACCA	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29280_Mgig_Bot2012_COI	PLO-A05	1	BD29280_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	TCTTATTCGTTGGAGACTTTATAACCCT	135.87604
BD29280	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71782	WGS84	BD29280	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29280_Mjap_Gib2022_COI	PLO-A05	1	BD29280_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29280	Sample	BD29280	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A05	COI	45	BD29280	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A05	AATTTAATTGTGACTAGGCATGGTCTG	135.87604
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29281_Cmae_Bot2011_COI	PLO-A04	1	BD29281_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Cmae_Bot2011_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	ATGAACAGTCTATCCTCCTTTAG	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29281_Hsan_Wil2021_COI	PLO-A04	1	BD29281_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Hsan_Wil2021_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	CCTGGGCCGGTATAGTAGGT	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29281_Mros_Gib2022_COI	PLO-A04	1	BD29281_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Mros_Gib2022_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	ACTTCTACCTCCTGCTCTTCTACTTCTC	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29281_Msal_Bot2012_COI	PLO-A04	1	BD29281_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Msal_Bot2012_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	GYTAGTTCCRATGATGTTAGCTG	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29281_Mstr_Wil2021_COI	PLO-A04	1	BD29281_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Mstr_Wil2021_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	GTAGTGGTAACTACTCATGCTTTA	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29281_Pvir_Dia2013_COI	PLO-A04	1	BD29281_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Pvir_Dia2013_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	CTTAGTGGCATTAATTCGDAATCC	135.87711
BD29281	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.7194	WGS84	BD29281	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29281_Rhar_Sim2018_COI	PLO-A04	1	BD29281_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29281	Sample	BD29281	targeted	Absent	Sample	Rhar_Sim2018_COI	PLO-A04	COI	45	BD29281	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A04	CTGCTGCTATTGCTCATG	135.87711
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29283_Aamu_Bax2006_COI	PLO-A02	1	BD29283_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Aamu_Bax2006_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	GCACAACCGGGATCTTTACTTC	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29283_Pcan_Bot2011_IGS	PLO-A02	1	BD29283_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Pcan_Bot2011_IGS	PLO-A02	IGS	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	CGTAATCCTCAGTACTGGCTA	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29283_Pper_Dia2013_COI	PLO-A02	1	BD29283_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Pper_Dia2013_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	CTTAGTGGCATTAATTCGDAATCC	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	36.434	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29283_Sspa_Oph2007_COI	PLO-A02	1	BD29283_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Present	Sample	Sspa_Oph2007_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	GAAGGCCTTCCGATGATC	135.87965
BD29283	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71619	WGS84	BD29283	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29283_Upin_Bot2011_COI	PLO-A02	1	BD29283_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29283	Sample	BD29283	targeted	Absent	Sample	Upin_Bot2011_COI	PLO-A02	COI	45	BD29283	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A02	CTTTAATTACAGCGTTTTTATTGTTGT	135.87965
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29284_Cjap_Sim2018_COI	PLO-A01	1	BD29284_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Cjap_Sim2018_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29284_Esin_And2018_Cyb	PLO-A01	1	BD29284_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Esin_And2018_Cyb	PLO-A01	Cytb	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	ACCCCTCCTCATATCCAACCA	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29284_Mgig_Bot2012_COI	PLO-A01	1	BD29284_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Mgig_Bot2012_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	TCTTATTCGTTGGAGACTTTATAACCCT	135.8807
BD29284	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14380	-34.71776	WGS84	BD29284	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29284_Mjap_Gib2022_COI	PLO-A01	1	BD29284_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29284	Sample	BD29284	targeted	Absent	Sample	Mjap_Gib2022_COI	PLO-A01	COI	45	BD29284	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PLO-A01	AATTTAATTGTGACTAGGCATGGTCTG	135.8807
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29285_Cmae_Bot2011_COI	POR-A35	1	BD29285_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	ATGAACAGTCTATCCTCCTTTAG	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29285_Hsan_Wil2021_COI	POR-A35	1	BD29285_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	CCTGGGCCGGTATAGTAGGT	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	34.588	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29285_Mros_Gib2022_COI	POR-A35	1	BD29285_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Present	Sample	Mros_Gib2022_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29285_Msal_Bot2012_COI	POR-A35	1	BD29285_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	GYTAGTTCCRATGATGTTAGCTG	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29285_Mstr_Wil2021_COI	POR-A35	1	BD29285_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	GTAGTGGTAACTACTCATGCTTTA	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29285_Pvir_Dia2013_COI	POR-A35	1	BD29285_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	CTTAGTGGCATTAATTCGDAATCC	141.61391
BD29285	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34511	WGS84	BD29285	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29285_Rhar_Sim2018_COI	POR-A35	1	BD29285_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29285	Sample	BD29285	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A35	COI	45	BD29285	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A35	CTGCTGCTATTGCTCATG	141.61391
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29287_Aamu_Bax2006_COI	POR-A33	1	BD29287_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	GCACAACCGGGATCTTTACTTC	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29287_Pcan_Bot2011_IGS	POR-A33	1	BD29287_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A33	IGS	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	CGTAATCCTCAGTACTGGCTA	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29287_Pper_Dia2013_COI	POR-A33	1	BD29287_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	CTTAGTGGCATTAATTCGDAATCC	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29287_Sspa_Oph2007_COI	POR-A33	1	BD29287_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	GAAGGCCTTCCGATGATC	141.61111
BD29287	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34488	WGS84	BD29287	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.197	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29287_Upin_Bot2011_COI	POR-A33	1	BD29287_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29287	Sample	BD29287	targeted	Present	Sample	Upin_Bot2011_COI	POR-A33	COI	45	BD29287	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A33	CTTTAATTACAGCGTTTTTATTGTTGT	141.61111
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29288_Cjap_Sim2018_COI	POR-A32	1	BD29288_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29288_Esin_And2018_Cyb	POR-A32	1	BD29288_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A32	Cytb	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	ACCCCTCCTCATATCCAACCA	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29288_Mgig_Bot2012_COI	POR-A32	1	BD29288_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	TCTTATTCGTTGGAGACTTTATAACCCT	141.62275
BD29288	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34508	WGS84	BD29288	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29288_Mjap_Gib2022_COI	POR-A32	1	BD29288_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29288	Sample	BD29288	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A32	COI	45	BD29288	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A32	AATTTAATTGTGACTAGGCATGGTCTG	141.62275
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29289_Cmae_Bot2011_COI	POR-A31	1	BD29289_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	ATGAACAGTCTATCCTCCTTTAG	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29289_Hsan_Wil2021_COI	POR-A31	1	BD29289_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	CCTGGGCCGGTATAGTAGGT	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29289_Mros_Gib2022_COI	POR-A31	1	BD29289_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29289_Msal_Bot2012_COI	POR-A31	1	BD29289_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	GYTAGTTCCRATGATGTTAGCTG	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29289_Mstr_Wil2021_COI	POR-A31	1	BD29289_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	GTAGTGGTAACTACTCATGCTTTA	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29289_Pvir_Dia2013_COI	POR-A31	1	BD29289_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	CTTAGTGGCATTAATTCGDAATCC	141.62149
BD29289	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34531	WGS84	BD29289	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29289_Rhar_Sim2018_COI	POR-A31	1	BD29289_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29289	Sample	BD29289	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A31	COI	45	BD29289	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A31	CTGCTGCTATTGCTCATG	141.62149
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29290_Aamu_Bax2006_COI	POR-A30	1	BD29290_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	GCACAACCGGGATCTTTACTTC	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29290_Pcan_Bot2011_IGS	POR-A30	1	BD29290_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A30	IGS	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	CGTAATCCTCAGTACTGGCTA	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29290_Pper_Dia2013_COI	POR-A30	1	BD29290_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	CTTAGTGGCATTAATTCGDAATCC	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	34.639	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29290_Sspa_Oph2007_COI	POR-A30	1	BD29290_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	GAAGGCCTTCCGATGATC	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	33.661	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29290_Upin_Bot2011_COI	POR-A30	1	BD29290_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Present	Sample	Upin_Bot2011_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	CTTTAATTACAGCGTTTTTATTGTTGT	141.61982
BD29290	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34555	WGS84	BD29290	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29290_Vgib_Wil2025_COI	POR-A30	1	BD29290_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29290	Sample	BD29290	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A30	COI	45	BD29290	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A30	CCCCTCTTTACAAGTTCTTTAGGGT	141.61982
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29291_Asen_Wil2025_COI	POR-A29	1	BD29291_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	GTTGTTGTTACAACACATGCTCTTTTG	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29291_Cjap_Sim2018_COI	POR-A29	1	BD29291_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29291_Esin_And2018_Cyb	POR-A29	1	BD29291_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A29	Cytb	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	ACCCCTCCTCATATCCAACCA	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29291_Mgig_Bot2012_COI	POR-A29	1	BD29291_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	TCTTATTCGTTGGAGACTTTATAACCCT	141.61874
BD29291	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34545	WGS84	BD29291	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29291_Mjap_Gib2022_COI	POR-A29	1	BD29291_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29291	Sample	BD29291	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A29	COI	45	BD29291	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A29	AATTTAATTGTGACTAGGCATGGTCTG	141.61874
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29292_Cmae_Bot2011_COI	POR-A28	1	BD29292_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	ATGAACAGTCTATCCTCCTTTAG	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29292_Hsan_Wil2021_COI	POR-A28	1	BD29292_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	CCTGGGCCGGTATAGTAGGT	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29292_Mros_Gib2022_COI	POR-A28	1	BD29292_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29292_Msal_Bot2012_COI	POR-A28	1	BD29292_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	GYTAGTTCCRATGATGTTAGCTG	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29292_Mstr_Wil2021_COI	POR-A28	1	BD29292_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	GTAGTGGTAACTACTCATGCTTTA	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29292_Pvir_Dia2013_COI	POR-A28	1	BD29292_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	CTTAGTGGCATTAATTCGDAATCC	141.61735
BD29292	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34554	WGS84	BD29292	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29292_Rhar_Sim2018_COI	POR-A28	1	BD29292_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29292	Sample	BD29292	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A28	COI	45	BD29292	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A28	CTGCTGCTATTGCTCATG	141.61735
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29294_Aamu_Bax2006_COI	POR-A26	1	BD29294_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	GCACAACCGGGATCTTTACTTC	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29294_Pcan_Bot2011_IGS	POR-A26	1	BD29294_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A26	IGS	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	CGTAATCCTCAGTACTGGCTA	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29294_Pper_Dia2013_COI	POR-A26	1	BD29294_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	CTTAGTGGCATTAATTCGDAATCC	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.441	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29294_Sspa_Oph2007_COI	POR-A26	1	BD29294_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	GAAGGCCTTCCGATGATC	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	31.395	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29294_Upin_Bot2011_COI	POR-A26	1	BD29294_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Present	Sample	Upin_Bot2011_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	CTTTAATTACAGCGTTTTTATTGTTGT	141.61454
BD29294	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34566	WGS84	BD29294	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29294_Vgib_Wil2025_COI	POR-A26	1	BD29294_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29294	Sample	BD29294	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A26	COI	45	BD29294	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A26	CCCCTCTTTACAAGTTCTTTAGGGT	141.61454
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29295_Asen_Wil2025_COI	POR-A25	1	BD29295_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	GTTGTTGTTACAACACATGCTCTTTTG	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29295_Cjap_Sim2018_COI	POR-A25	1	BD29295_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29295_Esin_And2018_Cyb	POR-A25	1	BD29295_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A25	Cytb	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	ACCCCTCCTCATATCCAACCA	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29295_Mgig_Bot2012_COI	POR-A25	1	BD29295_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	TCTTATTCGTTGGAGACTTTATAACCCT	141.61313
BD29295	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34572	WGS84	BD29295	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29295_Mjap_Gib2022_COI	POR-A25	1	BD29295_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29295	Sample	BD29295	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A25	COI	45	BD29295	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A25	AATTTAATTGTGACTAGGCATGGTCTG	141.61313
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29296_Cmae_Bot2011_COI	POR-A24	1	BD29296_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	ATGAACAGTCTATCCTCCTTTAG	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29296_Hsan_Wil2021_COI	POR-A24	1	BD29296_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	CCTGGGCCGGTATAGTAGGT	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29296_Mros_Gib2022_COI	POR-A24	1	BD29296_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29296_Msal_Bot2012_COI	POR-A24	1	BD29296_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	GYTAGTTCCRATGATGTTAGCTG	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29296_Mstr_Wil2021_COI	POR-A24	1	BD29296_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	GTAGTGGTAACTACTCATGCTTTA	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29296_Pvir_Dia2013_COI	POR-A24	1	BD29296_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	CTTAGTGGCATTAATTCGDAATCC	141.6222
BD29296	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34618	WGS84	BD29296	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29296_Rhar_Sim2018_COI	POR-A24	1	BD29296_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29296	Sample	BD29296	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A24	COI	45	BD29296	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A24	CTGCTGCTATTGCTCATG	141.6222
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29298_Aamu_Bax2006_COI	POR-A22	1	BD29298_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	GCACAACCGGGATCTTTACTTC	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29298_Pcan_Bot2011_IGS	POR-A22	1	BD29298_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A22	IGS	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	CGTAATCCTCAGTACTGGCTA	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29298_Pper_Dia2013_COI	POR-A22	1	BD29298_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	CTTAGTGGCATTAATTCGDAATCC	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29298_Sspa_Oph2007_COI	POR-A22	1	BD29298_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	GAAGGCCTTCCGATGATC	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.417	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29298_Upin_Bot2011_COI	POR-A22	1	BD29298_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Present	Sample	Upin_Bot2011_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	CTTTAATTACAGCGTTTTTATTGTTGT	141.61961
BD29298	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34641	WGS84	BD29298	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29298_Vgib_Wil2025_COI	POR-A22	1	BD29298_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29298	Sample	BD29298	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A22	COI	45	BD29298	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A22	CCCCTCTTTACAAGTTCTTTAGGGT	141.61961
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29299_Asen_Wil2025_COI	POR-A21	1	BD29299_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	GTTGTTGTTACAACACATGCTCTTTTG	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29299_Cjap_Sim2018_COI	POR-A21	1	BD29299_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29299_Esin_And2018_Cyb	POR-A21	1	BD29299_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A21	Cytb	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	ACCCCTCCTCATATCCAACCA	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29299_Mgig_Bot2012_COI	POR-A21	1	BD29299_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	TCTTATTCGTTGGAGACTTTATAACCCT	141.61823
BD29299	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34646	WGS84	BD29299	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29299_Mjap_Gib2022_COI	POR-A21	1	BD29299_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29299	Sample	BD29299	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A21	COI	45	BD29299	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A21	AATTTAATTGTGACTAGGCATGGTCTG	141.61823
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29300_Cmae_Bot2011_COI	POR-A20	1	BD29300_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	ATGAACAGTCTATCCTCCTTTAG	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29300_Hsan_Wil2021_COI	POR-A20	1	BD29300_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	CCTGGGCCGGTATAGTAGGT	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29300_Mros_Gib2022_COI	POR-A20	1	BD29300_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29300_Msal_Bot2012_COI	POR-A20	1	BD29300_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	GYTAGTTCCRATGATGTTAGCTG	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29300_Mstr_Wil2021_COI	POR-A20	1	BD29300_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	GTAGTGGTAACTACTCATGCTTTA	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29300_Pvir_Dia2013_COI	POR-A20	1	BD29300_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	CTTAGTGGCATTAATTCGDAATCC	141.61686
BD29300	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34654	WGS84	BD29300	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29300_Rhar_Sim2018_COI	POR-A20	1	BD29300_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29300	Sample	BD29300	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A20	COI	45	BD29300	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A20	CTGCTGCTATTGCTCATG	141.61686
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29302_Aamu_Bax2006_COI	POR-A18	1	BD29302_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	GCACAACCGGGATCTTTACTTC	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29302_Pcan_Bot2011_IGS	POR-A18	1	BD29302_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A18	IGS	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	CGTAATCCTCAGTACTGGCTA	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29302_Pper_Dia2013_COI	POR-A18	1	BD29302_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	CTTAGTGGCATTAATTCGDAATCC	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	35.242	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29302_Sspa_Oph2007_COI	POR-A18	1	BD29302_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	GAAGGCCTTCCGATGATC	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	29.602	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29302_Upin_Bot2011_COI	POR-A18	1	BD29302_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Present	Sample	Upin_Bot2011_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	CTTTAATTACAGCGTTTTTATTGTTGT	141.61393
BD29302	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD29302	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29302_Vgib_Wil2025_COI	POR-A18	1	BD29302_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29302	Sample	BD29302	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A18	COI	45	BD29302	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A18	CCCCTCTTTACAAGTTCTTTAGGGT	141.61393
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29303_Asen_Wil2025_COI	POR-A17	1	BD29303_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	GTTGTTGTTACAACACATGCTCTTTTG	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29303_Cjap_Sim2018_COI	POR-A17	1	BD29303_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29303_Esin_And2018_Cyb	POR-A17	1	BD29303_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A17	Cytb	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	ACCCCTCCTCATATCCAACCA	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29303_Mgig_Bot2012_COI	POR-A17	1	BD29303_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	TCTTATTCGTTGGAGACTTTATAACCCT	141.61256
BD29303	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34672	WGS84	BD29303	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29303_Mjap_Gib2022_COI	POR-A17	1	BD29303_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29303	Sample	BD29303	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A17	COI	45	BD29303	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A17	AATTTAATTGTGACTAGGCATGGTCTG	141.61256
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29304_Cmae_Bot2011_COI	POR-A16	1	BD29304_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	ATGAACAGTCTATCCTCCTTTAG	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29304_Hsan_Wil2021_COI	POR-A16	1	BD29304_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	CCTGGGCCGGTATAGTAGGT	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29304_Mros_Gib2022_COI	POR-A16	1	BD29304_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29304_Msal_Bot2012_COI	POR-A16	1	BD29304_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	GYTAGTTCCRATGATGTTAGCTG	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29304_Mstr_Wil2021_COI	POR-A16	1	BD29304_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	GTAGTGGTAACTACTCATGCTTTA	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29304_Pvir_Dia2013_COI	POR-A16	1	BD29304_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	CTTAGTGGCATTAATTCGDAATCC	141.62101
BD29304	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3479	WGS84	BD29304	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29304_Rhar_Sim2018_COI	POR-A16	1	BD29304_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29304	Sample	BD29304	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A16	COI	45	BD29304	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A16	CTGCTGCTATTGCTCATG	141.62101
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29306_Aamu_Bax2006_COI	POR-A14	1	BD29306_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	GCACAACCGGGATCTTTACTTC	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29306_Pcan_Bot2011_IGS	POR-A14	1	BD29306_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A14	IGS	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	CGTAATCCTCAGTACTGGCTA	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29306_Pper_Dia2013_COI	POR-A14	1	BD29306_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	CTTAGTGGCATTAATTCGDAATCC	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	38.559	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29306_Sspa_Oph2007_COI	POR-A14	1	BD29306_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Present	Sample	Sspa_Oph2007_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	GAAGGCCTTCCGATGATC	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.027	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29306_Upin_Bot2011_COI	POR-A14	1	BD29306_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Present	Sample	Upin_Bot2011_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	CTTTAATTACAGCGTTTTTATTGTTGT	141.61892
BD29306	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3474	WGS84	BD29306	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29306_Vgib_Wil2025_COI	POR-A14	1	BD29306_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29306	Sample	BD29306	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A14	COI	45	BD29306	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A14	CCCCTCTTTACAAGTTCTTTAGGGT	141.61892
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29307_Asen_Wil2025_COI	POR-A13	1	BD29307_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	GTTGTTGTTACAACACATGCTCTTTTG	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29307_Cjap_Sim2018_COI	POR-A13	1	BD29307_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29307_Esin_And2018_Cyb	POR-A13	1	BD29307_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A13	Cytb	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	ACCCCTCCTCATATCCAACCA	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29307_Mgig_Bot2012_COI	POR-A13	1	BD29307_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	TCTTATTCGTTGGAGACTTTATAACCCT	141.61754
BD29307	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34743	WGS84	BD29307	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29307_Mjap_Gib2022_COI	POR-A13	1	BD29307_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29307	Sample	BD29307	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A13	COI	45	BD29307	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A13	AATTTAATTGTGACTAGGCATGGTCTG	141.61754
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29308_Cmae_Bot2011_COI	POR-A12	1	BD29308_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	ATGAACAGTCTATCCTCCTTTAG	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29308_Hsan_Wil2021_COI	POR-A12	1	BD29308_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	CCTGGGCCGGTATAGTAGGT	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29308_Mros_Gib2022_COI	POR-A12	1	BD29308_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29308_Msal_Bot2012_COI	POR-A12	1	BD29308_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	GYTAGTTCCRATGATGTTAGCTG	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29308_Mstr_Wil2021_COI	POR-A12	1	BD29308_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	GTAGTGGTAACTACTCATGCTTTA	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29308_Pvir_Dia2013_COI	POR-A12	1	BD29308_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	CTTAGTGGCATTAATTCGDAATCC	141.61615
BD29308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34754	WGS84	BD29308	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29308_Rhar_Sim2018_COI	POR-A12	1	BD29308_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29308	Sample	BD29308	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A12	COI	45	BD29308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A12	CTGCTGCTATTGCTCATG	141.61615
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD29310_Asen_Wil2025_COI	POR-A10	1	BD29310_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Asen_Wil2025_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	GTTGTTGTTACAACACATGCTCTTTTG	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29310_Cjap_Sim2018_COI	POR-A10	1	BD29310_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29310_Esin_And2018_Cyb	POR-A10	1	BD29310_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A10	Cytb	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	ACCCCTCCTCATATCCAACCA	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29310_Mgig_Bot2012_COI	POR-A10	1	BD29310_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	TCTTATTCGTTGGAGACTTTATAACCCT	141.61328
BD29310	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3476	WGS84	BD29310	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29310_Mjap_Gib2022_COI	POR-A10	1	BD29310_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29310	Sample	BD29310	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A10	COI	45	BD29310	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A10	AATTTAATTGTGACTAGGCATGGTCTG	141.61328
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29311_Cmae_Bot2011_COI	POR-A09	1	BD29311_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	ATGAACAGTCTATCCTCCTTTAG	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29311_Hsan_Wil2021_COI	POR-A09	1	BD29311_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	CCTGGGCCGGTATAGTAGGT	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29311_Mros_Gib2022_COI	POR-A09	1	BD29311_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29311_Msal_Bot2012_COI	POR-A09	1	BD29311_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	GYTAGTTCCRATGATGTTAGCTG	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29311_Mstr_Wil2021_COI	POR-A09	1	BD29311_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	GTAGTGGTAACTACTCATGCTTTA	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29311_Pvir_Dia2013_COI	POR-A09	1	BD29311_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	CTTAGTGGCATTAATTCGDAATCC	141.61189
BD29311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34768	WGS84	BD29311	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29311_Rhar_Sim2018_COI	POR-A09	1	BD29311_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29311	Sample	BD29311	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A09	COI	45	BD29311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A09	CTGCTGCTATTGCTCATG	141.61189
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29313_Aamu_Bax2006_COI	POR-A07	1	BD29313_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	GCACAACCGGGATCTTTACTTC	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29313_Pcan_Bot2011_IGS	POR-A07	1	BD29313_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A07	IGS	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	CGTAATCCTCAGTACTGGCTA	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29313_Pper_Dia2013_COI	POR-A07	1	BD29313_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	CTTAGTGGCATTAATTCGDAATCC	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29313_Sspa_Oph2007_COI	POR-A07	1	BD29313_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	GAAGGCCTTCCGATGATC	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.78	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29313_Upin_Bot2011_COI	POR-A07	1	BD29313_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Present	Sample	Upin_Bot2011_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	CTTTAATTACAGCGTTTTTATTGTTGT	141.62051
BD29313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34881	WGS84	BD29313	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD29313_Vgib_Wil2025_COI	POR-A07	1	BD29313_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29313	Sample	BD29313	targeted	Absent	Sample	Vgib_Wil2025_COI	POR-A07	COI	45	BD29313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A07	CCCCTCTTTACAAGTTCTTTAGGGT	141.62051
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29314_Cjap_Sim2018_COI	POR-A06	1	BD29314_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29314_Esin_And2018_Cyb	POR-A06	1	BD29314_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A06	Cytb	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	ACCCCTCCTCATATCCAACCA	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29314_Mgig_Bot2012_COI	POR-A06	1	BD29314_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	TCTTATTCGTTGGAGACTTTATAACCCT	141.61969
BD29314	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34832	WGS84	BD29314	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29314_Mjap_Gib2022_COI	POR-A06	1	BD29314_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29314	Sample	BD29314	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A06	COI	45	BD29314	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A06	AATTTAATTGTGACTAGGCATGGTCTG	141.61969
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29315_Cmae_Bot2011_COI	POR-A05	1	BD29315_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	ATGAACAGTCTATCCTCCTTTAG	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29315_Hsan_Wil2021_COI	POR-A05	1	BD29315_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	CCTGGGCCGGTATAGTAGGT	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	33.964	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29315_Mros_Gib2022_COI	POR-A05	1	BD29315_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Present	Sample	Mros_Gib2022_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29315_Msal_Bot2012_COI	POR-A05	1	BD29315_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	GYTAGTTCCRATGATGTTAGCTG	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29315_Mstr_Wil2021_COI	POR-A05	1	BD29315_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	GTAGTGGTAACTACTCATGCTTTA	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29315_Pvir_Dia2013_COI	POR-A05	1	BD29315_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	CTTAGTGGCATTAATTCGDAATCC	141.61829
BD29315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3484	WGS84	BD29315	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29315_Rhar_Sim2018_COI	POR-A05	1	BD29315_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29315	Sample	BD29315	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A05	COI	45	BD29315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A05	CTGCTGCTATTGCTCATG	141.61829
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29317_Aamu_Bax2006_COI	POR-A03	1	BD29317_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	GCACAACCGGGATCTTTACTTC	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29317_Pcan_Bot2011_IGS	POR-A03	1	BD29317_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-A03	IGS	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	CGTAATCCTCAGTACTGGCTA	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29317_Pper_Dia2013_COI	POR-A03	1	BD29317_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Pper_Dia2013_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	CTTAGTGGCATTAATTCGDAATCC	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29317_Sspa_Oph2007_COI	POR-A03	1	BD29317_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	GAAGGCCTTCCGATGATC	141.61122
BD29317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34866	WGS84	BD29317	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	35.484	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29317_Upin_Bot2011_COI	POR-A03	1	BD29317_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29317	Sample	BD29317	targeted	Present	Sample	Upin_Bot2011_COI	POR-A03	COI	45	BD29317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A03	CTTTAATTACAGCGTTTTTATTGTTGT	141.61122
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29318_Cjap_Sim2018_COI	POR-A02	1	BD29318_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29318_Esin_And2018_Cyb	POR-A02	1	BD29318_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Esin_And2018_Cyb	POR-A02	Cytb	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	ACCCCTCCTCATATCCAACCA	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29318_Mgig_Bot2012_COI	POR-A02	1	BD29318_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	TCTTATTCGTTGGAGACTTTATAACCCT	141.62044
BD29318	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34947	WGS84	BD29318	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/10/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29318_Mjap_Gib2022_COI	POR-A02	1	BD29318_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29318	Sample	BD29318	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-A02	COI	45	BD29318	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A02	AATTTAATTGTGACTAGGCATGGTCTG	141.62044
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29319_Cmae_Bot2011_COI	POR-A01	1	BD29319_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	ATGAACAGTCTATCCTCCTTTAG	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29319_Hsan_Wil2021_COI	POR-A01	1	BD29319_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	CCTGGGCCGGTATAGTAGGT	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29319_Mros_Gib2022_COI	POR-A01	1	BD29319_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Mros_Gib2022_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29319_Msal_Bot2012_COI	POR-A01	1	BD29319_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Msal_Bot2012_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	GYTAGTTCCRATGATGTTAGCTG	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29319_Mstr_Wil2021_COI	POR-A01	1	BD29319_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	GTAGTGGTAACTACTCATGCTTTA	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29319_Pvir_Dia2013_COI	POR-A01	1	BD29319_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	CTTAGTGGCATTAATTCGDAATCC	141.61035
BD29319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34935	WGS84	BD29319	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Winter	9/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29319_Rhar_Sim2018_COI	POR-A01	1	BD29319_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29319	Sample	BD29319	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-A01	COI	45	BD29319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-A01	CTGCTGCTATTGCTCATG	141.61035
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29320_Aamu_Bax2006_COI	KPT-A35	1	BD29320_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	GCACAACCGGGATCTTTACTTC	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29320_Pcan_Bot2011_IGS	KPT-A35	1	BD29320_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A35	IGS	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	CGTAATCCTCAGTACTGGCTA	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29320_Pper_Dia2013_COI	KPT-A35	1	BD29320_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	CTTAGTGGCATTAATTCGDAATCC	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29320_Sspa_Oph2007_COI	KPT-A35	1	BD29320_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	GAAGGCCTTCCGATGATC	137.77845
BD29320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95766	WGS84	BD29320	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29320_Upin_Bot2011_COI	KPT-A35	1	BD29320_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29320	Sample	BD29320	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A35	COI	45	BD29320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A35	CTTTAATTACAGCGTTTTTATTGTTGT	137.77845
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29321_Cjap_Sim2018_COI	KPT-A34	1	BD29321_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29321_Esin_And2018_Cyb	KPT-A34	1	BD29321_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A34	Cytb	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	ACCCCTCCTCATATCCAACCA	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29321_Mgig_Bot2012_COI	KPT-A34	1	BD29321_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	TCTTATTCGTTGGAGACTTTATAACCCT	137.7771
BD29321	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95762	WGS84	BD29321	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29321_Mjap_Gib2022_COI	KPT-A34	1	BD29321_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29321	Sample	BD29321	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A34	COI	45	BD29321	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A34	AATTTAATTGTGACTAGGCATGGTCTG	137.7771
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29322_Cmae_Bot2011_COI	KPT-A33	1	BD29322_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	ATGAACAGTCTATCCTCCTTTAG	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29322_Hsan_Wil2021_COI	KPT-A33	1	BD29322_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	CCTGGGCCGGTATAGTAGGT	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29322_Mros_Gib2022_COI	KPT-A33	1	BD29322_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29322_Msal_Bot2012_COI	KPT-A33	1	BD29322_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	GYTAGTTCCRATGATGTTAGCTG	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29322_Mstr_Wil2021_COI	KPT-A33	1	BD29322_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	GTAGTGGTAACTACTCATGCTTTA	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29322_Pvir_Dia2013_COI	KPT-A33	1	BD29322_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	CTTAGTGGCATTAATTCGDAATCC	137.77745
BD29322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95838	WGS84	BD29322	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29322_Rhar_Sim2018_COI	KPT-A33	1	BD29322_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29322	Sample	BD29322	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A33	COI	45	BD29322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A33	CTGCTGCTATTGCTCATG	137.77745
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29324_Aamu_Bax2006_COI	KPT-A31	1	BD29324_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	GCACAACCGGGATCTTTACTTC	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29324_Pcan_Bot2011_IGS	KPT-A31	1	BD29324_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A31	IGS	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	CGTAATCCTCAGTACTGGCTA	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29324_Pper_Dia2013_COI	KPT-A31	1	BD29324_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	CTTAGTGGCATTAATTCGDAATCC	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29324_Sspa_Oph2007_COI	KPT-A31	1	BD29324_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	GAAGGCCTTCCGATGATC	137.77984
BD29324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95957	WGS84	BD29324	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29324_Upin_Bot2011_COI	KPT-A31	1	BD29324_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29324	Sample	BD29324	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A31	COI	45	BD29324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A31	CTTTAATTACAGCGTTTTTATTGTTGT	137.77984
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29325_Cjap_Sim2018_COI	KPT-A30	1	BD29325_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29325_Esin_And2018_Cyb	KPT-A30	1	BD29325_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A30	Cytb	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	ACCCCTCCTCATATCCAACCA	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29325_Mgig_Bot2012_COI	KPT-A30	1	BD29325_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	TCTTATTCGTTGGAGACTTTATAACCCT	137.77845
BD29325	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95959	WGS84	BD29325	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29325_Mjap_Gib2022_COI	KPT-A30	1	BD29325_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29325	Sample	BD29325	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A30	COI	45	BD29325	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A30	AATTTAATTGTGACTAGGCATGGTCTG	137.77845
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29326_Cmae_Bot2011_COI	KPT-A29	1	BD29326_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	ATGAACAGTCTATCCTCCTTTAG	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29326_Hsan_Wil2021_COI	KPT-A29	1	BD29326_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	CCTGGGCCGGTATAGTAGGT	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29326_Mros_Gib2022_COI	KPT-A29	1	BD29326_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29326_Msal_Bot2012_COI	KPT-A29	1	BD29326_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	GYTAGTTCCRATGATGTTAGCTG	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29326_Mstr_Wil2021_COI	KPT-A29	1	BD29326_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	GTAGTGGTAACTACTCATGCTTTA	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29326_Pvir_Dia2013_COI	KPT-A29	1	BD29326_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	CTTAGTGGCATTAATTCGDAATCC	137.77712
BD29326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.95973	WGS84	BD29326	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29326_Rhar_Sim2018_COI	KPT-A29	1	BD29326_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29326	Sample	BD29326	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A29	COI	45	BD29326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A29	CTGCTGCTATTGCTCATG	137.77712
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29328_Aamu_Bax2006_COI	KPT-A27	1	BD29328_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	GCACAACCGGGATCTTTACTTC	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29328_Pcan_Bot2011_IGS	KPT-A27	1	BD29328_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A27	IGS	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	CGTAATCCTCAGTACTGGCTA	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29328_Pper_Dia2013_COI	KPT-A27	1	BD29328_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	CTTAGTGGCATTAATTCGDAATCC	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29328_Sspa_Oph2007_COI	KPT-A27	1	BD29328_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	GAAGGCCTTCCGATGATC	137.77676
BD29328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96035	WGS84	BD29328	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29328_Upin_Bot2011_COI	KPT-A27	1	BD29328_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29328	Sample	BD29328	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A27	COI	45	BD29328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A27	CTTTAATTACAGCGTTTTTATTGTTGT	137.77676
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29329_Cjap_Sim2018_COI	KPT-A26	1	BD29329_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29329_Esin_And2018_Cyb	KPT-A26	1	BD29329_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A26	Cytb	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	ACCCCTCCTCATATCCAACCA	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29329_Mgig_Bot2012_COI	KPT-A26	1	BD29329_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	TCTTATTCGTTGGAGACTTTATAACCCT	137.77811
BD29329	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96018	WGS84	BD29329	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29329_Mjap_Gib2022_COI	KPT-A26	1	BD29329_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29329	Sample	BD29329	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A26	COI	45	BD29329	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A26	AATTTAATTGTGACTAGGCATGGTCTG	137.77811
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29331_Aamu_Bax2006_COI	KPT-A24	1	BD29331_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	GCACAACCGGGATCTTTACTTC	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29331_Pcan_Bot2011_IGS	KPT-A24	1	BD29331_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A24	IGS	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	CGTAATCCTCAGTACTGGCTA	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29331_Pper_Dia2013_COI	KPT-A24	1	BD29331_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	CTTAGTGGCATTAATTCGDAATCC	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29331_Sspa_Oph2007_COI	KPT-A24	1	BD29331_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	GAAGGCCTTCCGATGATC	137.78109
BD29331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.9613	WGS84	BD29331	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29331_Upin_Bot2011_COI	KPT-A24	1	BD29331_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29331	Sample	BD29331	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A24	COI	45	BD29331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A24	CTTTAATTACAGCGTTTTTATTGTTGT	137.78109
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29332_Cjap_Sim2018_COI	KPT-A23	1	BD29332_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29332_Esin_And2018_Cyb	KPT-A23	1	BD29332_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A23	Cytb	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	ACCCCTCCTCATATCCAACCA	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29332_Mgig_Bot2012_COI	KPT-A23	1	BD29332_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	TCTTATTCGTTGGAGACTTTATAACCCT	137.77976
BD29332	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96149	WGS84	BD29332	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29332_Mjap_Gib2022_COI	KPT-A23	1	BD29332_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29332	Sample	BD29332	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A23	COI	45	BD29332	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A23	AATTTAATTGTGACTAGGCATGGTCTG	137.77976
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29333_Cmae_Bot2011_COI	KPT-A22	1	BD29333_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	ATGAACAGTCTATCCTCCTTTAG	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29333_Hsan_Wil2021_COI	KPT-A22	1	BD29333_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	CCTGGGCCGGTATAGTAGGT	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29333_Mros_Gib2022_COI	KPT-A22	1	BD29333_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29333_Msal_Bot2012_COI	KPT-A22	1	BD29333_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	GYTAGTTCCRATGATGTTAGCTG	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29333_Mstr_Wil2021_COI	KPT-A22	1	BD29333_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	GTAGTGGTAACTACTCATGCTTTA	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29333_Pvir_Dia2013_COI	KPT-A22	1	BD29333_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	CTTAGTGGCATTAATTCGDAATCC	137.77849
BD29333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96147	WGS84	BD29333	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29333_Rhar_Sim2018_COI	KPT-A22	1	BD29333_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29333	Sample	BD29333	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A22	COI	45	BD29333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A22	CTGCTGCTATTGCTCATG	137.77849
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29335_Aamu_Bax2006_COI	KPT-A20	1	BD29335_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	GCACAACCGGGATCTTTACTTC	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29335_Pcan_Bot2011_IGS	KPT-A20	1	BD29335_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A20	IGS	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	CGTAATCCTCAGTACTGGCTA	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29335_Pper_Dia2013_COI	KPT-A20	1	BD29335_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	CTTAGTGGCATTAATTCGDAATCC	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29335_Sspa_Oph2007_COI	KPT-A20	1	BD29335_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	GAAGGCCTTCCGATGATC	137.77572
BD29335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96196	WGS84	BD29335	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29335_Upin_Bot2011_COI	KPT-A20	1	BD29335_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29335	Sample	BD29335	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A20	COI	45	BD29335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A20	CTTTAATTACAGCGTTTTTATTGTTGT	137.77572
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29336_Cjap_Sim2018_COI	KPT-A19	1	BD29336_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29336_Esin_And2018_Cyb	KPT-A19	1	BD29336_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A19	Cytb	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	ACCCCTCCTCATATCCAACCA	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29336_Mgig_Bot2012_COI	KPT-A19	1	BD29336_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	TCTTATTCGTTGGAGACTTTATAACCCT	137.77707
BD29336	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96212	WGS84	BD29336	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29336_Mjap_Gib2022_COI	KPT-A19	1	BD29336_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29336	Sample	BD29336	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A19	COI	45	BD29336	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A19	AATTTAATTGTGACTAGGCATGGTCTG	137.77707
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29337_Cmae_Bot2011_COI	KPT-A18	1	BD29337_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	ATGAACAGTCTATCCTCCTTTAG	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29337_Hsan_Wil2021_COI	KPT-A18	1	BD29337_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	CCTGGGCCGGTATAGTAGGT	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29337_Mros_Gib2022_COI	KPT-A18	1	BD29337_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29337_Msal_Bot2012_COI	KPT-A18	1	BD29337_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	GYTAGTTCCRATGATGTTAGCTG	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29337_Mstr_Wil2021_COI	KPT-A18	1	BD29337_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	GTAGTGGTAACTACTCATGCTTTA	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29337_Pvir_Dia2013_COI	KPT-A18	1	BD29337_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	CTTAGTGGCATTAATTCGDAATCC	137.7782
BD29337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96198	WGS84	BD29337	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29337_Rhar_Sim2018_COI	KPT-A18	1	BD29337_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29337	Sample	BD29337	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A18	COI	45	BD29337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A18	CTGCTGCTATTGCTCATG	137.7782
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29339_Aamu_Bax2006_COI	KPT-A16	1	BD29339_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	GCACAACCGGGATCTTTACTTC	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29339_Pcan_Bot2011_IGS	KPT-A16	1	BD29339_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A16	IGS	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	CGTAATCCTCAGTACTGGCTA	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29339_Pper_Dia2013_COI	KPT-A16	1	BD29339_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	CTTAGTGGCATTAATTCGDAATCC	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29339_Sspa_Oph2007_COI	KPT-A16	1	BD29339_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	GAAGGCCTTCCGATGATC	137.78114
BD29339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96324	WGS84	BD29339	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29339_Upin_Bot2011_COI	KPT-A16	1	BD29339_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29339	Sample	BD29339	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A16	COI	45	BD29339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A16	CTTTAATTACAGCGTTTTTATTGTTGT	137.78114
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29340_Cmae_Bot2011_COI	KPT-A15	1	BD29340_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	ATGAACAGTCTATCCTCCTTTAG	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29340_Hsan_Wil2021_COI	KPT-A15	1	BD29340_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	CCTGGGCCGGTATAGTAGGT	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29340_Mros_Gib2022_COI	KPT-A15	1	BD29340_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29340_Msal_Bot2012_COI	KPT-A15	1	BD29340_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	GYTAGTTCCRATGATGTTAGCTG	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29340_Mstr_Wil2021_COI	KPT-A15	1	BD29340_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	GTAGTGGTAACTACTCATGCTTTA	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29340_Pvir_Dia2013_COI	KPT-A15	1	BD29340_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	CTTAGTGGCATTAATTCGDAATCC	137.77982
BD29340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96334	WGS84	BD29340	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29340_Rhar_Sim2018_COI	KPT-A15	1	BD29340_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29340	Sample	BD29340	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A15	COI	45	BD29340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A15	CTGCTGCTATTGCTCATG	137.77982
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29342_Aamu_Bax2006_COI	KPT-A13	1	BD29342_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	GCACAACCGGGATCTTTACTTC	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29342_Pcan_Bot2011_IGS	KPT-A13	1	BD29342_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A13	IGS	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	CGTAATCCTCAGTACTGGCTA	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29342_Pper_Dia2013_COI	KPT-A13	1	BD29342_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	CTTAGTGGCATTAATTCGDAATCC	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29342_Sspa_Oph2007_COI	KPT-A13	1	BD29342_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	GAAGGCCTTCCGATGATC	137.77723
BD29342	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96342	WGS84	BD29342	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29342_Upin_Bot2011_COI	KPT-A13	1	BD29342_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29342	Sample	BD29342	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A13	COI	45	BD29342	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A13	CTTTAATTACAGCGTTTTTATTGTTGT	137.77723
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29343_Cjap_Sim2018_COI	KPT-A12	1	BD29343_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29343_Esin_And2018_Cyb	KPT-A12	1	BD29343_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A12	Cytb	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	ACCCCTCCTCATATCCAACCA	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29343_Mgig_Bot2012_COI	KPT-A12	1	BD29343_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	TCTTATTCGTTGGAGACTTTATAACCCT	137.77589
BD29343	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96357	WGS84	BD29343	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29343_Mjap_Gib2022_COI	KPT-A12	1	BD29343_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29343	Sample	BD29343	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A12	COI	45	BD29343	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A12	AATTTAATTGTGACTAGGCATGGTCTG	137.77589
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29344_Hsan_Wil2021_COI	KPT-A11	1	BD29344_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	CCTGGGCCGGTATAGTAGGT	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29344_Mros_Gib2022_COI	KPT-A11	1	BD29344_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29344_Msal_Bot2012_COI	KPT-A11	1	BD29344_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	GYTAGTTCCRATGATGTTAGCTG	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29344_Mstr_Wil2021_COI	KPT-A11	1	BD29344_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	GTAGTGGTAACTACTCATGCTTTA	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29344_Pvir_Dia2013_COI	KPT-A11	1	BD29344_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	CTTAGTGGCATTAATTCGDAATCC	137.77455
BD29344	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96284	WGS84	BD29344	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29344_Rhar_Sim2018_COI	KPT-A11	1	BD29344_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29344	Sample	BD29344	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A11	COI	45	BD29344	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A11	CTGCTGCTATTGCTCATG	137.77455
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29346_Aamu_Bax2006_COI	KPT-A09	1	BD29346_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	GCACAACCGGGATCTTTACTTC	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29346_Pcan_Bot2011_IGS	KPT-A09	1	BD29346_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A09	IGS	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	CGTAATCCTCAGTACTGGCTA	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29346_Pper_Dia2013_COI	KPT-A09	1	BD29346_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	CTTAGTGGCATTAATTCGDAATCC	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29346_Sspa_Oph2007_COI	KPT-A09	1	BD29346_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	GAAGGCCTTCCGATGATC	137.7745
BD29346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96384	WGS84	BD29346	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29346_Upin_Bot2011_COI	KPT-A09	1	BD29346_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29346	Sample	BD29346	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A09	COI	45	BD29346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A09	CTTTAATTACAGCGTTTTTATTGTTGT	137.7745
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29347_Cjap_Sim2018_COI	KPT-A08	1	BD29347_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29347_Esin_And2018_Cyb	KPT-A08	1	BD29347_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A08	Cytb	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	ACCCCTCCTCATATCCAACCA	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29347_Mgig_Bot2012_COI	KPT-A08	1	BD29347_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	TCTTATTCGTTGGAGACTTTATAACCCT	137.77573
BD29347	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29347	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29347_Mjap_Gib2022_COI	KPT-A08	1	BD29347_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29347	Sample	BD29347	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A08	COI	45	BD29347	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A08	AATTTAATTGTGACTAGGCATGGTCTG	137.77573
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29348_Cmae_Bot2011_COI	KPT-A07	1	BD29348_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	ATGAACAGTCTATCCTCCTTTAG	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29348_Hsan_Wil2021_COI	KPT-A07	1	BD29348_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	CCTGGGCCGGTATAGTAGGT	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29348_Mros_Gib2022_COI	KPT-A07	1	BD29348_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29348_Msal_Bot2012_COI	KPT-A07	1	BD29348_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	GYTAGTTCCRATGATGTTAGCTG	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29348_Mstr_Wil2021_COI	KPT-A07	1	BD29348_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	GTAGTGGTAACTACTCATGCTTTA	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29348_Pvir_Dia2013_COI	KPT-A07	1	BD29348_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	CTTAGTGGCATTAATTCGDAATCC	137.77697
BD29348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96386	WGS84	BD29348	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29348_Rhar_Sim2018_COI	KPT-A07	1	BD29348_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29348	Sample	BD29348	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A07	COI	45	BD29348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A07	CTGCTGCTATTGCTCATG	137.77697
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29350_Cjap_Sim2018_COI	KPT-A05	1	BD29350_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29350_Esin_And2018_Cyb	KPT-A05	1	BD29350_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Esin_And2018_Cyb	KPT-A05	Cytb	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	ACCCCTCCTCATATCCAACCA	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29350_Mgig_Bot2012_COI	KPT-A05	1	BD29350_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Mgig_Bot2012_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	TCTTATTCGTTGGAGACTTTATAACCCT	137.78049
BD29350	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96427	WGS84	BD29350	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29350_Mjap_Gib2022_COI	KPT-A05	1	BD29350_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29350	Sample	BD29350	targeted	Absent	Sample	Mjap_Gib2022_COI	KPT-A05	COI	45	BD29350	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A05	AATTTAATTGTGACTAGGCATGGTCTG	137.78049
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29351_Cmae_Bot2011_COI	KPT-A04	1	BD29351_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Cmae_Bot2011_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	ATGAACAGTCTATCCTCCTTTAG	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29351_Hsan_Wil2021_COI	KPT-A04	1	BD29351_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Hsan_Wil2021_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	CCTGGGCCGGTATAGTAGGT	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29351_Mros_Gib2022_COI	KPT-A04	1	BD29351_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Mros_Gib2022_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29351_Msal_Bot2012_COI	KPT-A04	1	BD29351_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Msal_Bot2012_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	GYTAGTTCCRATGATGTTAGCTG	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29351_Mstr_Wil2021_COI	KPT-A04	1	BD29351_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Mstr_Wil2021_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	GTAGTGGTAACTACTCATGCTTTA	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29351_Pvir_Dia2013_COI	KPT-A04	1	BD29351_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Pvir_Dia2013_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	CTTAGTGGCATTAATTCGDAATCC	137.77854
BD29351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96531	WGS84	BD29351	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29351_Rhar_Sim2018_COI	KPT-A04	1	BD29351_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29351	Sample	BD29351	targeted	Absent	Sample	Rhar_Sim2018_COI	KPT-A04	COI	45	BD29351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A04	CTGCTGCTATTGCTCATG	137.77854
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29353_Aamu_Bax2006_COI	KPT-A02	1	BD29353_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Aamu_Bax2006_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	GCACAACCGGGATCTTTACTTC	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29353_Pcan_Bot2011_IGS	KPT-A02	1	BD29353_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Pcan_Bot2011_IGS	KPT-A02	IGS	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	CGTAATCCTCAGTACTGGCTA	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29353_Pper_Dia2013_COI	KPT-A02	1	BD29353_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Pper_Dia2013_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	CTTAGTGGCATTAATTCGDAATCC	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29353_Sspa_Oph2007_COI	KPT-A02	1	BD29353_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Sspa_Oph2007_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	GAAGGCCTTCCGATGATC	137.77582
BD29353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-34.96544	WGS84	BD29353	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29353_Upin_Bot2011_COI	KPT-A02	1	BD29353_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29353	Sample	BD29353	targeted	Absent	Sample	Upin_Bot2011_COI	KPT-A02	COI	45	BD29353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A02	CTTTAATTACAGCGTTTTTATTGTTGT	137.77582
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29354_Cjap_Sim2018_COI	PGI-A35	1	BD29354_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29354_Esin_And2018_Cyb	PGI-A35	1	BD29354_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A35	Cytb	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	ACCCCTCCTCATATCCAACCA	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29354_Mgig_Bot2012_COI	PGI-A35	1	BD29354_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	TCTTATTCGTTGGAGACTTTATAACCCT	137.76478
BD29354	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.025	WGS84	BD29354	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29354_Mjap_Gib2022_COI	PGI-A35	1	BD29354_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29354	Sample	BD29354	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A35	COI	45	BD29354	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A35	AATTTAATTGTGACTAGGCATGGTCTG	137.76478
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29355_Cmae_Bot2011_COI	PGI-A34	1	BD29355_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	ATGAACAGTCTATCCTCCTTTAG	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29355_Hsan_Wil2021_COI	PGI-A34	1	BD29355_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	CCTGGGCCGGTATAGTAGGT	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29355_Mros_Gib2022_COI	PGI-A34	1	BD29355_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29355_Msal_Bot2012_COI	PGI-A34	1	BD29355_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	GYTAGTTCCRATGATGTTAGCTG	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29355_Mstr_Wil2021_COI	PGI-A34	1	BD29355_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	GTAGTGGTAACTACTCATGCTTTA	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29355_Pvir_Dia2013_COI	PGI-A34	1	BD29355_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	CTTAGTGGCATTAATTCGDAATCC	137.76598
BD29355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02458	WGS84	BD29355	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29355_Rhar_Sim2018_COI	PGI-A34	1	BD29355_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29355	Sample	BD29355	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A34	COI	45	BD29355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A34	CTGCTGCTATTGCTCATG	137.76598
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29357_Aamu_Bax2006_COI	PGI-A32	1	BD29357_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	GCACAACCGGGATCTTTACTTC	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29357_Pcan_Bot2011_IGS	PGI-A32	1	BD29357_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A32	IGS	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	CGTAATCCTCAGTACTGGCTA	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29357_Pper_Dia2013_COI	PGI-A32	1	BD29357_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	CTTAGTGGCATTAATTCGDAATCC	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29357_Sspa_Oph2007_COI	PGI-A32	1	BD29357_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	GAAGGCCTTCCGATGATC	137.76976
BD29357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02466	WGS84	BD29357	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29357_Upin_Bot2011_COI	PGI-A32	1	BD29357_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29357	Sample	BD29357	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A32	COI	45	BD29357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A32	CTTTAATTACAGCGTTTTTATTGTTGT	137.76976
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29358_Cjap_Sim2018_COI	PGI-A31	1	BD29358_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29358_Esin_And2018_Cyb	PGI-A31	1	BD29358_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A31	Cytb	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	ACCCCTCCTCATATCCAACCA	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29358_Mgig_Bot2012_COI	PGI-A31	1	BD29358_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	TCTTATTCGTTGGAGACTTTATAACCCT	137.77129
BD29358	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02464	WGS84	BD29358	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29358_Mjap_Gib2022_COI	PGI-A31	1	BD29358_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29358	Sample	BD29358	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A31	COI	45	BD29358	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A31	AATTTAATTGTGACTAGGCATGGTCTG	137.77129
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29359_Cmae_Bot2011_COI	PGI-A30	1	BD29359_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	ATGAACAGTCTATCCTCCTTTAG	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29359_Hsan_Wil2021_COI	PGI-A30	1	BD29359_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	CCTGGGCCGGTATAGTAGGT	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29359_Mros_Gib2022_COI	PGI-A30	1	BD29359_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29359_Msal_Bot2012_COI	PGI-A30	1	BD29359_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	GYTAGTTCCRATGATGTTAGCTG	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29359_Mstr_Wil2021_COI	PGI-A30	1	BD29359_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	GTAGTGGTAACTACTCATGCTTTA	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29359_Pvir_Dia2013_COI	PGI-A30	1	BD29359_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	CTTAGTGGCATTAATTCGDAATCC	137.77228
BD29359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02338	WGS84	BD29359	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29359_Rhar_Sim2018_COI	PGI-A30	1	BD29359_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29359	Sample	BD29359	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A30	COI	45	BD29359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A30	CTGCTGCTATTGCTCATG	137.77228
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29360_Aamu_Bax2006_COI	PGI-A29	1	BD29360_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	GCACAACCGGGATCTTTACTTC	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29360_Pcan_Bot2011_IGS	PGI-A29	1	BD29360_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A29	IGS	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	CGTAATCCTCAGTACTGGCTA	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29360_Pper_Dia2013_COI	PGI-A29	1	BD29360_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	CTTAGTGGCATTAATTCGDAATCC	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29360_Sspa_Oph2007_COI	PGI-A29	1	BD29360_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	GAAGGCCTTCCGATGATC	137.77066
BD29360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0234	WGS84	BD29360	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29360_Upin_Bot2011_COI	PGI-A29	1	BD29360_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29360	Sample	BD29360	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A29	COI	45	BD29360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A29	CTTTAATTACAGCGTTTTTATTGTTGT	137.77066
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29361_Cjap_Sim2018_COI	PGI-A28	1	BD29361_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29361_Esin_And2018_Cyb	PGI-A28	1	BD29361_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A28	Cytb	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	ACCCCTCCTCATATCCAACCA	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29361_Mgig_Bot2012_COI	PGI-A28	1	BD29361_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	TCTTATTCGTTGGAGACTTTATAACCCT	137.76915
BD29361	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02377	WGS84	BD29361	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29361_Mjap_Gib2022_COI	PGI-A28	1	BD29361_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29361	Sample	BD29361	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A28	COI	45	BD29361	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A28	AATTTAATTGTGACTAGGCATGGTCTG	137.76915
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29362_Cmae_Bot2011_COI	PGI-A27	1	BD29362_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	ATGAACAGTCTATCCTCCTTTAG	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29362_Hsan_Wil2021_COI	PGI-A27	1	BD29362_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	CCTGGGCCGGTATAGTAGGT	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29362_Mros_Gib2022_COI	PGI-A27	1	BD29362_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29362_Msal_Bot2012_COI	PGI-A27	1	BD29362_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	GYTAGTTCCRATGATGTTAGCTG	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29362_Mstr_Wil2021_COI	PGI-A27	1	BD29362_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	GTAGTGGTAACTACTCATGCTTTA	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29362_Pvir_Dia2013_COI	PGI-A27	1	BD29362_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	CTTAGTGGCATTAATTCGDAATCC	137.76747
BD29362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02384	WGS84	BD29362	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29362_Rhar_Sim2018_COI	PGI-A27	1	BD29362_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29362	Sample	BD29362	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A27	COI	45	BD29362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A27	CTGCTGCTATTGCTCATG	137.76747
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29364_Aamu_Bax2006_COI	PGI-A25	1	BD29364_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	GCACAACCGGGATCTTTACTTC	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29364_Pcan_Bot2011_IGS	PGI-A25	1	BD29364_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A25	IGS	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	CGTAATCCTCAGTACTGGCTA	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29364_Pper_Dia2013_COI	PGI-A25	1	BD29364_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	CTTAGTGGCATTAATTCGDAATCC	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29364_Sspa_Oph2007_COI	PGI-A25	1	BD29364_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	GAAGGCCTTCCGATGATC	137.76465
BD29364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02271	WGS84	BD29364	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29364_Upin_Bot2011_COI	PGI-A25	1	BD29364_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29364	Sample	BD29364	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A25	COI	45	BD29364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A25	CTTTAATTACAGCGTTTTTATTGTTGT	137.76465
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29365_Cjap_Sim2018_COI	PGI-A24	1	BD29365_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29365_Esin_And2018_Cyb	PGI-A24	1	BD29365_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A24	Cytb	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	ACCCCTCCTCATATCCAACCA	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29365_Mgig_Bot2012_COI	PGI-A24	1	BD29365_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	TCTTATTCGTTGGAGACTTTATAACCCT	137.76629
BD29365	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02273	WGS84	BD29365	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29365_Mjap_Gib2022_COI	PGI-A24	1	BD29365_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29365	Sample	BD29365	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A24	COI	45	BD29365	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A24	AATTTAATTGTGACTAGGCATGGTCTG	137.76629
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29366_Cmae_Bot2011_COI	PGI-A23	1	BD29366_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	ATGAACAGTCTATCCTCCTTTAG	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29366_Hsan_Wil2021_COI	PGI-A23	1	BD29366_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	CCTGGGCCGGTATAGTAGGT	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29366_Mros_Gib2022_COI	PGI-A23	1	BD29366_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29366_Msal_Bot2012_COI	PGI-A23	1	BD29366_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	GYTAGTTCCRATGATGTTAGCTG	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29366_Mstr_Wil2021_COI	PGI-A23	1	BD29366_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	GTAGTGGTAACTACTCATGCTTTA	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29366_Pvir_Dia2013_COI	PGI-A23	1	BD29366_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	CTTAGTGGCATTAATTCGDAATCC	137.76796
BD29366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02252	WGS84	BD29366	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29366_Rhar_Sim2018_COI	PGI-A23	1	BD29366_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29366	Sample	BD29366	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A23	COI	45	BD29366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A23	CTGCTGCTATTGCTCATG	137.76796
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29368_Aamu_Bax2006_COI	PGI-A21	1	BD29368_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	GCACAACCGGGATCTTTACTTC	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29368_Pcan_Bot2011_IGS	PGI-A21	1	BD29368_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A21	IGS	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	CGTAATCCTCAGTACTGGCTA	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29368_Pper_Dia2013_COI	PGI-A21	1	BD29368_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	CTTAGTGGCATTAATTCGDAATCC	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29368_Sspa_Oph2007_COI	PGI-A21	1	BD29368_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	GAAGGCCTTCCGATGATC	137.77254
BD29368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0219	WGS84	BD29368	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29368_Upin_Bot2011_COI	PGI-A21	1	BD29368_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29368	Sample	BD29368	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A21	COI	45	BD29368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A21	CTTTAATTACAGCGTTTTTATTGTTGT	137.77254
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29369_Cjap_Sim2018_COI	PGI-A20	1	BD29369_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29369_Esin_And2018_Cyb	PGI-A20	1	BD29369_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A20	Cytb	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	ACCCCTCCTCATATCCAACCA	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29369_Mgig_Bot2012_COI	PGI-A20	1	BD29369_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	TCTTATTCGTTGGAGACTTTATAACCCT	137.77337
BD29369	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02194	WGS84	BD29369	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29369_Mjap_Gib2022_COI	PGI-A20	1	BD29369_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29369	Sample	BD29369	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A20	COI	45	BD29369	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A20	AATTTAATTGTGACTAGGCATGGTCTG	137.77337
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29371_Aamu_Bax2006_COI	PGI-A18	1	BD29371_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	GCACAACCGGGATCTTTACTTC	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29371_Pcan_Bot2011_IGS	PGI-A18	1	BD29371_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A18	IGS	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	CGTAATCCTCAGTACTGGCTA	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29371_Pper_Dia2013_COI	PGI-A18	1	BD29371_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	CTTAGTGGCATTAATTCGDAATCC	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29371_Sspa_Oph2007_COI	PGI-A18	1	BD29371_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	GAAGGCCTTCCGATGATC	137.77238
BD29371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02137	WGS84	BD29371	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29371_Upin_Bot2011_COI	PGI-A18	1	BD29371_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29371	Sample	BD29371	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A18	COI	45	BD29371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A18	CTTTAATTACAGCGTTTTTATTGTTGT	137.77238
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29372_Cjap_Sim2018_COI	PGI-A17	1	BD29372_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29372_Esin_And2018_Cyb	PGI-A17	1	BD29372_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A17	Cytb	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	ACCCCTCCTCATATCCAACCA	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29372_Mgig_Bot2012_COI	PGI-A17	1	BD29372_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	TCTTATTCGTTGGAGACTTTATAACCCT	137.77262
BD29372	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02158	WGS84	BD29372	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29372_Mjap_Gib2022_COI	PGI-A17	1	BD29372_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29372	Sample	BD29372	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A17	COI	45	BD29372	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A17	AATTTAATTGTGACTAGGCATGGTCTG	137.77262
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29373_Cmae_Bot2011_COI	PGI-A16	1	BD29373_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	ATGAACAGTCTATCCTCCTTTAG	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29373_Hsan_Wil2021_COI	PGI-A16	1	BD29373_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	CCTGGGCCGGTATAGTAGGT	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29373_Mros_Gib2022_COI	PGI-A16	1	BD29373_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29373_Msal_Bot2012_COI	PGI-A16	1	BD29373_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	GYTAGTTCCRATGATGTTAGCTG	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29373_Mstr_Wil2021_COI	PGI-A16	1	BD29373_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	GTAGTGGTAACTACTCATGCTTTA	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29373_Pvir_Dia2013_COI	PGI-A16	1	BD29373_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	CTTAGTGGCATTAATTCGDAATCC	137.77345
BD29373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02031	WGS84	BD29373	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29373_Rhar_Sim2018_COI	PGI-A16	1	BD29373_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29373	Sample	BD29373	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A16	COI	45	BD29373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A16	CTGCTGCTATTGCTCATG	137.77345
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29375_Aamu_Bax2006_COI	PGI-A14	1	BD29375_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	GCACAACCGGGATCTTTACTTC	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29375_Pcan_Bot2011_IGS	PGI-A14	1	BD29375_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A14	IGS	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	CGTAATCCTCAGTACTGGCTA	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29375_Pper_Dia2013_COI	PGI-A14	1	BD29375_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	CTTAGTGGCATTAATTCGDAATCC	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29375_Sspa_Oph2007_COI	PGI-A14	1	BD29375_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	GAAGGCCTTCCGATGATC	137.76969
BD29375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02034	WGS84	BD29375	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29375_Upin_Bot2011_COI	PGI-A14	1	BD29375_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29375	Sample	BD29375	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A14	COI	45	BD29375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A14	CTTTAATTACAGCGTTTTTATTGTTGT	137.76969
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29376_Cjap_Sim2018_COI	PGI-A13	1	BD29376_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29376_Esin_And2018_Cyb	PGI-A13	1	BD29376_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A13	Cytb	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	ACCCCTCCTCATATCCAACCA	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29376_Mgig_Bot2012_COI	PGI-A13	1	BD29376_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	TCTTATTCGTTGGAGACTTTATAACCCT	137.76788
BD29376	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02029	WGS84	BD29376	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29376_Mjap_Gib2022_COI	PGI-A13	1	BD29376_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29376	Sample	BD29376	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A13	COI	45	BD29376	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A13	AATTTAATTGTGACTAGGCATGGTCTG	137.76788
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29377_Cmae_Bot2011_COI	PGI-A12	1	BD29377_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	ATGAACAGTCTATCCTCCTTTAG	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29377_Hsan_Wil2021_COI	PGI-A12	1	BD29377_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	CCTGGGCCGGTATAGTAGGT	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29377_Mros_Gib2022_COI	PGI-A12	1	BD29377_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29377_Msal_Bot2012_COI	PGI-A12	1	BD29377_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	GYTAGTTCCRATGATGTTAGCTG	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29377_Mstr_Wil2021_COI	PGI-A12	1	BD29377_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	GTAGTGGTAACTACTCATGCTTTA	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29377_Pvir_Dia2013_COI	PGI-A12	1	BD29377_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	CTTAGTGGCATTAATTCGDAATCC	137.7662
BD29377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.02043	WGS84	BD29377	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29377_Rhar_Sim2018_COI	PGI-A12	1	BD29377_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29377	Sample	BD29377	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A12	COI	45	BD29377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A12	CTGCTGCTATTGCTCATG	137.7662
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29379_Aamu_Bax2006_COI	PGI-A10	1	BD29379_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	GCACAACCGGGATCTTTACTTC	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29379_Pcan_Bot2011_IGS	PGI-A10	1	BD29379_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A10	IGS	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	CGTAATCCTCAGTACTGGCTA	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29379_Pper_Dia2013_COI	PGI-A10	1	BD29379_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	CTTAGTGGCATTAATTCGDAATCC	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29379_Sspa_Oph2007_COI	PGI-A10	1	BD29379_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	GAAGGCCTTCCGATGATC	137.76517
BD29379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.0189	WGS84	BD29379	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29379_Upin_Bot2011_COI	PGI-A10	1	BD29379_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29379	Sample	BD29379	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A10	COI	45	BD29379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A10	CTTTAATTACAGCGTTTTTATTGTTGT	137.76517
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29380_Cmae_Bot2011_COI	PGI-A09	1	BD29380_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	ATGAACAGTCTATCCTCCTTTAG	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29380_Hsan_Wil2021_COI	PGI-A09	1	BD29380_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	CCTGGGCCGGTATAGTAGGT	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29380_Mros_Gib2022_COI	PGI-A09	1	BD29380_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29380_Msal_Bot2012_COI	PGI-A09	1	BD29380_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	GYTAGTTCCRATGATGTTAGCTG	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29380_Pvir_Dia2013_COI	PGI-A09	1	BD29380_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	CTTAGTGGCATTAATTCGDAATCC	137.76659
BD29380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01862	WGS84	BD29380	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29380_Rhar_Sim2018_COI	PGI-A09	1	BD29380_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29380	Sample	BD29380	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A09	COI	45	BD29380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A09	CTGCTGCTATTGCTCATG	137.76659
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29382_Aamu_Bax2006_COI	PGI-A07	1	BD29382_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	GCACAACCGGGATCTTTACTTC	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29382_Pcan_Bot2011_IGS	PGI-A07	1	BD29382_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A07	IGS	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	CGTAATCCTCAGTACTGGCTA	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29382_Pper_Dia2013_COI	PGI-A07	1	BD29382_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	CTTAGTGGCATTAATTCGDAATCC	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29382_Sspa_Oph2007_COI	PGI-A07	1	BD29382_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	GAAGGCCTTCCGATGATC	137.77035
BD29382	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01874	WGS84	BD29382	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29382_Upin_Bot2011_COI	PGI-A07	1	BD29382_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29382	Sample	BD29382	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A07	COI	45	BD29382	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A07	CTTTAATTACAGCGTTTTTATTGTTGT	137.77035
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29383_Cjap_Sim2018_COI	PGI-A06	1	BD29383_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29383_Esin_And2018_Cyb	PGI-A06	1	BD29383_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A06	Cytb	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	ACCCCTCCTCATATCCAACCA	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29383_Mgig_Bot2012_COI	PGI-A06	1	BD29383_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	TCTTATTCGTTGGAGACTTTATAACCCT	137.77222
BD29383	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01883	WGS84	BD29383	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29383_Mjap_Gib2022_COI	PGI-A06	1	BD29383_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29383	Sample	BD29383	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A06	COI	45	BD29383	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A06	AATTTAATTGTGACTAGGCATGGTCTG	137.77222
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29384_Cmae_Bot2011_COI	PGI-A02	1	BD29384_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	ATGAACAGTCTATCCTCCTTTAG	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29384_Hsan_Wil2021_COI	PGI-A02	1	BD29384_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	CCTGGGCCGGTATAGTAGGT	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29384_Mros_Gib2022_COI	PGI-A02	1	BD29384_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29384_Msal_Bot2012_COI	PGI-A02	1	BD29384_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	GYTAGTTCCRATGATGTTAGCTG	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29384_Mstr_Wil2021_COI	PGI-A02	1	BD29384_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	GTAGTGGTAACTACTCATGCTTTA	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29384_Pvir_Dia2013_COI	PGI-A02	1	BD29384_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	CTTAGTGGCATTAATTCGDAATCC	137.76551
BD29384	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01729	WGS84	BD29384	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29384_Rhar_Sim2018_COI	PGI-A02	1	BD29384_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29384	Sample	BD29384	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A02	COI	45	BD29384	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A02	CTGCTGCTATTGCTCATG	137.76551
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD29386_Aamu_Bax2006_COI	PGI-A05	1	BD29386_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Aamu_Bax2006_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	GCACAACCGGGATCTTTACTTC	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD29386_Pcan_Bot2011_IGS	PGI-A05	1	BD29386_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Pcan_Bot2011_IGS	PGI-A05	IGS	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	CGTAATCCTCAGTACTGGCTA	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD29386_Pper_Dia2013_COI	PGI-A05	1	BD29386_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Pper_Dia2013_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	CTTAGTGGCATTAATTCGDAATCC	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD29386_Sspa_Oph2007_COI	PGI-A05	1	BD29386_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Sspa_Oph2007_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	GAAGGCCTTCCGATGATC	137.77132
BD29386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01792	WGS84	BD29386	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD29386_Upin_Bot2011_COI	PGI-A05	1	BD29386_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29386	Sample	BD29386	targeted	Absent	Sample	Upin_Bot2011_COI	PGI-A05	COI	45	BD29386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A05	CTTTAATTACAGCGTTTTTATTGTTGT	137.77132
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD29387_Cjap_Sim2018_COI	PGI-A04	1	BD29387_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Cjap_Sim2018_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD29387_Esin_And2018_Cyb	PGI-A04	1	BD29387_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Esin_And2018_Cyb	PGI-A04	Cytb	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	ACCCCTCCTCATATCCAACCA	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD29387_Mgig_Bot2012_COI	PGI-A04	1	BD29387_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Mgig_Bot2012_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	TCTTATTCGTTGGAGACTTTATAACCCT	137.7696
BD29387	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01815	WGS84	BD29387	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD29387_Mjap_Gib2022_COI	PGI-A04	1	BD29387_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29387	Sample	BD29387	targeted	Absent	Sample	Mjap_Gib2022_COI	PGI-A04	COI	45	BD29387	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A04	AATTTAATTGTGACTAGGCATGGTCTG	137.7696
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD29388_Cmae_Bot2011_COI	PGI-A01	1	BD29388_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Cmae_Bot2011_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	ATGAACAGTCTATCCTCCTTTAG	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD29388_Hsan_Wil2021_COI	PGI-A01	1	BD29388_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Hsan_Wil2021_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	CCTGGGCCGGTATAGTAGGT	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD29388_Mros_Gib2022_COI	PGI-A01	1	BD29388_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Mros_Gib2022_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	ACTTCTACCTCCTGCTCTTCTACTTCTC	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD29388_Msal_Bot2012_COI	PGI-A01	1	BD29388_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Msal_Bot2012_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	GYTAGTTCCRATGATGTTAGCTG	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD29388_Mstr_Wil2021_COI	PGI-A01	1	BD29388_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Mstr_Wil2021_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	GTAGTGGTAACTACTCATGCTTTA	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD29388_Pvir_Dia2013_COI	PGI-A01	1	BD29388_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Pvir_Dia2013_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	CTTAGTGGCATTAATTCGDAATCC	137.76432
BD29388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14379	-35.01848	WGS84	BD29388	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Port_Giles_Winter	10/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD29388_Rhar_Sim2018_COI	PGI-A01	1	BD29388_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD29388	Sample	BD29388	targeted	Absent	Sample	Rhar_Sim2018_COI	PGI-A01	COI	45	BD29388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	PGI-A01	CTGCTGCTATTGCTCATG	137.76432
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82164777	WGS84	BD42628	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42628_Pper_Dia2013_COI	ADV-02	1	BD42628_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-02	COI	45	BD42628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-02	CTTAGTGGCATTAATTCGDAATCC	144.9096108
BD42628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82164777	WGS84	BD42628	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42628_Vgib_Wil2025_COI	ADV-02	0	BD42628_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42628	Sample	BD42628	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-02	COI	45	BD42628	1.00E-05	Nil	Australia	ADV-02	CCCCTCTTTACAAGTTCTTTAGGGT	144.9096108
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82136752	WGS84	BD42629	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	37.172	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42629_Asen_Wil2025_COI	ADV-03	0	BD42629_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Present	Sample	Asen_Wil2025_COI	ADV-03	COI	45	BD42629	1.00E-05	Nil	Australia	ADV-03	GTTGTTGTTACAACACATGCTCTTTTG	144.9094286
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42629_Cjap_Sim2018_COI	ADV-03	1	BD42629_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-03	COI	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9094286
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42629_Esin_And2018_Cyb	ADV-03	1	BD42629_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-03	Cytb	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	ACCCCTCCTCATATCCAACCA	144.9094286
BD42629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82136752	WGS84	BD42629	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42629_Mjap_Gib2022_COI	ADV-03	1	BD42629_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42629	Sample	BD42629	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-03	COI	45	BD42629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-03	AATTTAATTGTGACTAGGCATGGTCTG	144.9094286
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8205268	WGS84	BD42631	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42631_Pper_Dia2013_COI	ADV-05	1	BD42631_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-05	COI	45	BD42631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-05	CTTAGTGGCATTAATTCGDAATCC	144.9088819
BD42631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8205268	WGS84	BD42631	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42631_Vgib_Wil2025_COI	ADV-05	0	BD42631_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42631	Sample	BD42631	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-05	COI	45	BD42631	1.00E-05	Nil	Australia	ADV-05	CCCCTCTTTACAAGTTCTTTAGGGT	144.9088819
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.8279042	WGS84	BD42632	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.347	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42632_Asen_Wil2025_COI	ADV-06	0	BD42632_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Present	Sample	Asen_Wil2025_COI	ADV-06	COI	45	BD42632	1.00E-05	Nil	Australia	ADV-06	GTTGTTGTTACAACACATGCTCTTTTG	144.8998675
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42632_Cjap_Sim2018_COI	ADV-06	1	BD42632_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-06	COI	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.8998675
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42632_Esin_And2018_Cyb	ADV-06	1	BD42632_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-06	Cytb	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	ACCCCTCCTCATATCCAACCA	144.8998675
BD42632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8279042	WGS84	BD42632	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42632_Mjap_Gib2022_COI	ADV-06	1	BD42632_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42632	Sample	BD42632	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-06	COI	45	BD42632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-06	AATTTAATTGTGACTAGGCATGGTCTG	144.8998675
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	31.253	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42633_Hsan_Wil2021_COI	ADV-07	1	BD42633_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-07	COI	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	CCTGGGCCGGTATAGTAGGT	144.8992714
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42633_Mros_Gib2022_COI	ADV-07	1	BD42633_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-07	COI	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.8992714
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42633_Pvir_Dia2013_COI	ADV-07	1	BD42633_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-07	COI	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	CTTAGTGGCATTAATTCGDAATCC	144.8992714
BD42633	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82764214	WGS84	BD42633	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42633_Rhar_Sim2018_COI	ADV-07	1	BD42633_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42633	Sample	BD42633	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-07	COI	45	BD42633	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-07	CTGCTGCTATTGCTCATG	144.8992714
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82803522	WGS84	BD42635	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42635_Pper_Dia2013_COI	ADV-09	1	BD42635_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-09	COI	45	BD42635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-09	CTTAGTGGCATTAATTCGDAATCC	144.9001655
BD42635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.82803522	WGS84	BD42635	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42635_Vgib_Wil2025_COI	ADV-09	0	BD42635_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42635	Sample	BD42635	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-09	COI	45	BD42635	1.00E-05	Nil	Australia	ADV-09	CCCCTCTTTACAAGTTCTTTAGGGT	144.9001655
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81817395	WGS84	BD42636	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42636_Asen_Wil2025_COI	ADV-10	0	BD42636_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-10	COI	45	BD42636	1.00E-05	Nil	Australia	ADV-10	GTTGTTGTTACAACACATGCTCTTTTG	144.9215243
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42636_Cjap_Sim2018_COI	ADV-10	1	BD42636_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-10	COI	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9215243
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42636_Esin_And2018_Cyb	ADV-10	1	BD42636_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-10	Cytb	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	ACCCCTCCTCATATCCAACCA	144.9215243
BD42636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81817395	WGS84	BD42636	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42636_Mjap_Gib2022_COI	ADV-10	1	BD42636_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42636	Sample	BD42636	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-10	COI	45	BD42636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-10	AATTTAATTGTGACTAGGCATGGTCTG	144.9215243
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	29.34	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42637_Hsan_Wil2021_COI	ADV-11	1	BD42637_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-11	COI	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	CCTGGGCCGGTATAGTAGGT	144.9004635
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42637_Mros_Gib2022_COI	ADV-11	1	BD42637_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-11	COI	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9004635
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42637_Pvir_Dia2013_COI	ADV-11	1	BD42637_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-11	COI	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	CTTAGTGGCATTAATTCGDAATCC	144.9004635
BD42637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.82816625	WGS84	BD42637	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42637_Rhar_Sim2018_COI	ADV-11	1	BD42637_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42637	Sample	BD42637	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-11	COI	45	BD42637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-11	CTGCTGCTATTGCTCATG	144.9004635
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84265504	WGS84	BD42639	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42639_Pper_Dia2013_COI	ADV-13	1	BD42639_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-13	COI	45	BD42639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-13	CTTAGTGGCATTAATTCGDAATCC	144.9298511
BD42639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84265504	WGS84	BD42639	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42639_Vgib_Wil2025_COI	ADV-13	0	BD42639_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42639	Sample	BD42639	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-13	COI	45	BD42639	1.00E-05	Nil	Australia	ADV-13	CCCCTCTTTACAAGTTCTTTAGGGT	144.9298511
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	32.161	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42640_Hsan_Wil2021_COI	ADV-14	1	BD42640_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-14	COI	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	CCTGGGCCGGTATAGTAGGT	144.9293007
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42640_Mros_Gib2022_COI	ADV-14	1	BD42640_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-14	COI	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9293007
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42640_Pvir_Dia2013_COI	ADV-14	1	BD42640_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-14	COI	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	CTTAGTGGCATTAATTCGDAATCC	144.9293007
BD42640	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84254362	WGS84	BD42640	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42640_Rhar_Sim2018_COI	ADV-14	1	BD42640_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42640	Sample	BD42640	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-14	COI	45	BD42640	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-14	CTGCTGCTATTGCTCATG	144.9293007
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84220947	WGS84	BD42642	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42642_Pper_Dia2013_COI	ADV-16	1	BD42642_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-16	COI	45	BD42642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-16	CTTAGTGGCATTAATTCGDAATCC	144.9276494
BD42642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84220947	WGS84	BD42642	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42642_Vgib_Wil2025_COI	ADV-16	0	BD42642_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42642	Sample	BD42642	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-16	COI	45	BD42642	1.00E-05	Nil	Australia	ADV-16	CCCCTCTTTACAAGTTCTTTAGGGT	144.9276494
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81869538	WGS84	BD42643	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42643_Asen_Wil2025_COI	ADV-17	0	BD42643_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Asen_Wil2025_COI	ADV-17	COI	45	BD42643	1.00E-05	Nil	Australia	ADV-17	GTTGTTGTTACAACACATGCTCTTTTG	144.9217039
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42643_Cjap_Sim2018_COI	ADV-17	1	BD42643_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-17	COI	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9217039
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42643_Esin_And2018_Cyb	ADV-17	1	BD42643_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-17	Cytb	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	ACCCCTCCTCATATCCAACCA	144.9217039
BD42643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81869538	WGS84	BD42643	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42643_Mjap_Gib2022_COI	ADV-17	1	BD42643_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42643	Sample	BD42643	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-17	COI	45	BD42643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-17	AATTTAATTGTGACTAGGCATGGTCTG	144.9217039
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42644_Hsan_Wil2021_COI	ADV-18	1	BD42644_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-18	COI	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	CCTGGGCCGGTATAGTAGGT	144.9216141
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42644_Mros_Gib2022_COI	ADV-18	1	BD42644_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-18	COI	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9216141
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42644_Pvir_Dia2013_COI	ADV-18	1	BD42644_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-18	COI	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	CTTAGTGGCATTAATTCGDAATCC	144.9216141
BD42644	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81843466	WGS84	BD42644	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42644_Rhar_Sim2018_COI	ADV-18	1	BD42644_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42644	Sample	BD42644	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-18	COI	45	BD42644	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-18	CTGCTGCTATTGCTCATG	144.9216141
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81921681	WGS84	BD42646	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42646_Pper_Dia2013_COI	ADV-20	1	BD42646_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-20	COI	45	BD42646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-20	CTTAGTGGCATTAATTCGDAATCC	144.9218836
BD42646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81921681	WGS84	BD42646	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42646_Vgib_Wil2025_COI	ADV-20	0	BD42646_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42646	Sample	BD42646	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-20	COI	45	BD42646	1.00E-05	Nil	Australia	ADV-20	CCCCTCTTTACAAGTTCTTTAGGGT	144.9218836
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81969679	WGS84	BD42647	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	37.671	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42647_Asen_Wil2025_COI	ADV-21	0	BD42647_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Present	Sample	Asen_Wil2025_COI	ADV-21	COI	45	BD42647	1.00E-05	Nil	Australia	ADV-21	GTTGTTGTTACAACACATGCTCTTTTG	144.9156239
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42647_Cjap_Sim2018_COI	ADV-21	1	BD42647_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-21	COI	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9156239
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42647_Esin_And2018_Cyb	ADV-21	1	BD42647_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-21	Cytb	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	ACCCCTCCTCATATCCAACCA	144.9156239
BD42647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81969679	WGS84	BD42647	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42647_Mjap_Gib2022_COI	ADV-21	1	BD42647_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42647	Sample	BD42647	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-21	COI	45	BD42647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-21	AATTTAATTGTGACTAGGCATGGTCTG	144.9156239
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	36.773	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42648_Hsan_Wil2021_COI	ADV-22	1	BD42648_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Present	Sample	Hsan_Wil2021_COI	ADV-22	COI	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	CCTGGGCCGGTATAGTAGGT	144.9153674
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42648_Mros_Gib2022_COI	ADV-22	1	BD42648_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-22	COI	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9153674
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42648_Pvir_Dia2013_COI	ADV-22	1	BD42648_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-22	COI	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	CTTAGTGGCATTAATTCGDAATCC	144.9153674
BD42648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81919597	WGS84	BD42648	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42648_Rhar_Sim2018_COI	ADV-22	1	BD42648_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42648	Sample	BD42648	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-22	COI	45	BD42648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-22	CTGCTGCTATTGCTCATG	144.9153674
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.81994715	WGS84	BD42650	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.251	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42650_Asen_Wil2025_COI	ADV-24	0	BD42650_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Present	Sample	Asen_Wil2025_COI	ADV-24	COI	45	BD42650	1.00E-05	Nil	Australia	ADV-24	GTTGTTGTTACAACACATGCTCTTTTG	144.9157521
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42650_Cjap_Sim2018_COI	ADV-24	1	BD42650_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-24	COI	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9157521
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42650_Esin_And2018_Cyb	ADV-24	1	BD42650_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-24	Cytb	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	ACCCCTCCTCATATCCAACCA	144.9157521
BD42650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.81994715	WGS84	BD42650	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42650_Mjap_Gib2022_COI	ADV-24	1	BD42650_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42650	Sample	BD42650	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-24	COI	45	BD42650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-24	AATTTAATTGTGACTAGGCATGGTCTG	144.9157521
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42651_Hsan_Wil2021_COI	ADV-25	1	BD42651_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-25	COI	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	CCTGGGCCGGTATAGTAGGT	144.9158804
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42651_Mros_Gib2022_COI	ADV-25	1	BD42651_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-25	COI	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9158804
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42651_Pvir_Dia2013_COI	ADV-25	1	BD42651_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-25	COI	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	CTTAGTGGCATTAATTCGDAATCC	144.9158804
BD42651	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8201976	WGS84	BD42651	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42651_Rhar_Sim2018_COI	ADV-25	1	BD42651_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42651	Sample	BD42651	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-25	COI	45	BD42651	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-25	CTGCTGCTATTGCTCATG	144.9158804
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84526043	WGS84	BD42653	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD42653_Pper_Dia2013_COI	ADV-29	1	BD42653_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Pper_Dia2013_COI	ADV-29	COI	45	BD42653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-29	CTTAGTGGCATTAATTCGDAATCC	144.9067328
BD42653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84526043	WGS84	BD42653	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD42653_Vgib_Wil2025_COI	ADV-29	0	BD42653_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42653	Sample	BD42653	targeted	Absent	Sample	Vgib_Wil2025_COI	ADV-29	COI	45	BD42653	1.00E-05	Nil	Australia	ADV-29	CCCCTCTTTACAAGTTCTTTAGGGT	144.9067328
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-37.84503161	WGS84	BD42654	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	37.553	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD42654_Asen_Wil2025_COI	ADV-28	0	BD42654_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Present	Sample	Asen_Wil2025_COI	ADV-28	COI	45	BD42654	1.00E-05	Nil	Australia	ADV-28	GTTGTTGTTACAACACATGCTCTTTTG	144.9073504
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD42654_Cjap_Sim2018_COI	ADV-28	1	BD42654_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Cjap_Sim2018_COI	ADV-28	COI	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	TTAATATACGGTCATTTGGTATGAGTATAGATC	144.9073504
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD42654_Esin_And2018_Cyb	ADV-28	1	BD42654_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Esin_And2018_Cyb	ADV-28	Cytb	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	ACCCCTCCTCATATCCAACCA	144.9073504
BD42654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.84503161	WGS84	BD42654	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD42654_Mjap_Gib2022_COI	ADV-28	1	BD42654_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42654	Sample	BD42654	targeted	Absent	Sample	Mjap_Gib2022_COI	ADV-28	COI	45	BD42654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-28	AATTTAATTGTGACTAGGCATGGTCTG	144.9073504
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD42655_Hsan_Wil2021_COI	ADV-27	1	BD42655_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Hsan_Wil2021_COI	ADV-27	COI	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	CCTGGGCCGGTATAGTAGGT	144.9076592
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD42655_Mros_Gib2022_COI	ADV-27	1	BD42655_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Mros_Gib2022_COI	ADV-27	COI	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	ACTTCTACCTCCTGCTCTTCTACTTCTC	144.9076592
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD42655_Pvir_Dia2013_COI	ADV-27	1	BD42655_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Pvir_Dia2013_COI	ADV-27	COI	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	CTTAGTGGCATTAATTCGDAATCC	144.9076592
BD42655	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14569	-37.8449172	WGS84	BD42655	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Hemigrapsus_validation	22/02/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD42655_Rhar_Sim2018_COI	ADV-27	1	BD42655_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 20 micron Plankton net	BD42655	Sample	BD42655	targeted	Absent	Sample	Rhar_Sim2018_COI	ADV-27	COI	45	BD42655	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ADV-27	CTGCTGCTATTGCTCATG	144.9076592
BD44107	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55408	WGS84	BD44107	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.652	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44107_Mros_Gib2022_COI	ESAV-211	1	BD44107_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44107	Sample	BD44107	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-211	COI	45	BD44107	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-211	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.8808
BD44110	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55502	WGS84	BD44110	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	37.61	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44110_Mros_Gib2022_COI	ESAV-214	1	BD44110_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44110	Sample	BD44110	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-214	COI	45	BD44110	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-214	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87684
BD44113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55757	WGS84	BD44113	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44113_Asen_Wil2025_COI	ESAV-217	0	BD44113_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44113	Sample	BD44113	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-217	COI	45	BD44113	1.00E-05	Nil	Australia	ESAV-217	GTTGTTGTTACAACACATGCTCTTTTG	147.87285
BD44113	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55757	WGS84	BD44113	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	25.828	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44113_Mjap_Gib2022_COI	ESAV-217	1	BD44113_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44113	Sample	BD44113	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-217	COI	45	BD44113	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-217	AATTTAATTGTGACTAGGCATGGTCTG	147.87285
BD44114	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5486	WGS84	BD44114	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	36.761	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44114_Mros_Gib2022_COI	ESAV-218	1	BD44114_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44114	Sample	BD44114	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-218	COI	45	BD44114	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-218	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.92963
BD44117	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.5204	WGS84	BD44117	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	29.847	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44117_Mjap_Gib2022_COI	ESAV-221	1	BD44117_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44117	Sample	BD44117	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-221	COI	45	BD44117	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-221	AATTTAATTGTGACTAGGCATGGTCTG	147.91448
BD44118	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51625	WGS84	BD44118	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	30.591	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44118_Mros_Gib2022_COI	ESAV-223	1	BD44118_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44118	Sample	BD44118	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-223	COI	45	BD44118	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-223	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.91345
BD44120	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.14877	WGS84	BD44120	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44120_Asen_Wil2025_COI	ESAV-203	0	BD44120_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44120	Sample	BD44120	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-203	COI	45	BD44120	1.00E-05	Nil	Australia	ESAV-203	GTTGTTGTTACAACACATGCTCTTTTG	147.28186
BD44121	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55372	WGS84	BD44121	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44121_Mros_Gib2022_COI	ESAV-227	1	BD44121_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44121	Sample	BD44121	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-227	COI	45	BD44121	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-227	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.88807
BD44124	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51226	WGS84	BD44124	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	25.471	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	16/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44124_Mjap_Gib2022_COI	ESAV-224	1	BD44124_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44124	Sample	BD44124	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-224	COI	45	BD44124	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-224	AATTTAATTGTGACTAGGCATGGTCTG	147.91468
BD44125	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15187	WGS84	BD44125	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44125_Mros_Gib2022_COI	ESAV-205	1	BD44125_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44125	Sample	BD44125	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-205	COI	45	BD44125	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-205	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28325
BD44127	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15435	WGS84	BD44127	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	32.253	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44127_Vgib_Wil2025_COI	ESAV-207	0	BD44127_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44127	Sample	BD44127	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-207	COI	45	BD44127	1.00E-05	Nil	Australia	ESAV-207	CCCCTCTTTACAAGTTCTTTAGGGT	147.2836
BD44128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1557	WGS84	BD44128	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44128_Asen_Wil2025_COI	ESAV-208	0	BD44128_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44128	Sample	BD44128	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-208	COI	45	BD44128	1.00E-05	Nil	Australia	ESAV-208	GTTGTTGTTACAACACATGCTCTTTTG	147.28187
BD44128	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1557	WGS84	BD44128	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44128_Mjap_Gib2022_COI	ESAV-208	1	BD44128_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44128	Sample	BD44128	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-208	COI	45	BD44128	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-208	AATTTAATTGTGACTAGGCATGGTCTG	147.28187
BD44129	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15662	WGS84	BD44129	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	35.46	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44129_Mros_Gib2022_COI	ESAV-209	1	BD44129_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44129	Sample	BD44129	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-209	COI	45	BD44129	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-209	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28031
BD44130	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15936	WGS84	BD44130	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	30.094	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	22/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44130_Vgib_Wil2025_COI	ESAV-210	0	BD44130_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44130	Sample	BD44130	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-210	COI	45	BD44130	1.00E-05	Nil	Australia	ESAV-210	CCCCTCTTTACAAGTTCTTTAGGGT	147.27943
BD44131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.154	WGS84	BD44131	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	35.047	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44131_Asen_Wil2025_COI	ESAV-191	0	BD44131_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44131	Sample	BD44131	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-191	COI	45	BD44131	1.00E-05	Nil	Australia	ESAV-191	GTTGTTGTTACAACACATGCTCTTTTG	147.28375
BD44131	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.154	WGS84	BD44131	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44131_Mjap_Gib2022_COI	ESAV-191	1	BD44131_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44131	Sample	BD44131	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-191	COI	45	BD44131	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-191	AATTTAATTGTGACTAGGCATGGTCTG	147.28375
BD44132	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1546	WGS84	BD44132	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.824	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44132_Mros_Gib2022_COI	ESAV-193	1	BD44132_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44132	Sample	BD44132	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-193	COI	45	BD44132	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-193	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28539
BD44134	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15551	WGS84	BD44134	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.81	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44134_Vgib_Wil2025_COI	ESAV-196	0	BD44134_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44134	Sample	BD44134	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-196	COI	45	BD44134	1.00E-05	Nil	Australia	ESAV-196	CCCCTCTTTACAAGTTCTTTAGGGT	147.289
BD44135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15543	WGS84	BD44135	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	34.418	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44135_Asen_Wil2025_COI	ESAV-197	0	BD44135_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44135	Sample	BD44135	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-197	COI	45	BD44135	1.00E-05	Nil	Australia	ESAV-197	GTTGTTGTTACAACACATGCTCTTTTG	147.29045
BD44135	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15543	WGS84	BD44135	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44135_Mjap_Gib2022_COI	ESAV-197	1	BD44135_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44135	Sample	BD44135	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-197	COI	45	BD44135	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-197	AATTTAATTGTGACTAGGCATGGTCTG	147.29045
BD44136	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15645	WGS84	BD44136	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.508	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Summer	3/07/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44136_Mros_Gib2022_COI	ESAV-199	1	BD44136_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44136	Sample	BD44136	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-199	COI	45	BD44136	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-199	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.29038
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15883	WGS84	BD44138	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44138_Pper_Dia2013_COI	ESAV-001	1	BD44138_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-001	COI	45	BD44138	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-001	CTTAGTGGCATTAATTCGDAATCC	147.24663
BD44138	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15883	WGS84	BD44138	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	33.46	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44138_Vgib_Wil2025_COI	ESAV-001	0	BD44138_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44138	Sample	BD44138	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-001	COI	45	BD44138	1.00E-05	Nil	Australia	ESAV-001	CCCCTCTTTACAAGTTCTTTAGGGT	147.24663
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15882	WGS84	BD44139	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44139_Asen_Wil2025_COI	ESAV-002	0	BD44139_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-002	COI	45	BD44139	1.00E-05	Nil	Australia	ESAV-002	GTTGTTGTTACAACACATGCTCTTTTG	147.24847
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44139_Cjap_Sim2018_COI	ESAV-002	1	BD44139_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-002	COI	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.24847
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44139_Esin_And2018_Cyb	ESAV-002	1	BD44139_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-002	Cytb	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	ACCCCTCCTCATATCCAACCA	147.24847
BD44139	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15882	WGS84	BD44139	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44139_Mjap_Gib2022_COI	ESAV-002	1	BD44139_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44139	Sample	BD44139	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-002	COI	45	BD44139	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-002	AATTTAATTGTGACTAGGCATGGTCTG	147.24847
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15967	WGS84	BD44141	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44141_Pper_Dia2013_COI	ESAV-004	1	BD44141_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-004	COI	45	BD44141	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-004	CTTAGTGGCATTAATTCGDAATCC	147.25625
BD44141	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15967	WGS84	BD44141	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	33.716	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44141_Vgib_Wil2025_COI	ESAV-004	0	BD44141_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44141	Sample	BD44141	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-004	COI	45	BD44141	1.00E-05	Nil	Australia	ESAV-004	CCCCTCTTTACAAGTTCTTTAGGGT	147.25625
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.1605	WGS84	BD44142	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	36.019	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44142_Asen_Wil2025_COI	ESAV-005	0	BD44142_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Present	Sample	Asen_Wil2025_COI	ESAV-005	COI	45	BD44142	1.00E-05	Nil	Australia	ESAV-005	GTTGTTGTTACAACACATGCTCTTTTG	147.25847
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44142_Cjap_Sim2018_COI	ESAV-005	1	BD44142_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-005	COI	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.25847
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44142_Esin_And2018_Cyb	ESAV-005	1	BD44142_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-005	Cytb	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	ACCCCTCCTCATATCCAACCA	147.25847
BD44142	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1605	WGS84	BD44142	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44142_Mjap_Gib2022_COI	ESAV-005	1	BD44142_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44142	Sample	BD44142	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-005	COI	45	BD44142	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-005	AATTTAATTGTGACTAGGCATGGTCTG	147.25847
BD44143	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.1618	WGS84	BD44143	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.548	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/03/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44143_Mros_Gib2022_COI	ESAV-006	1	BD44143_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44143	Sample	BD44143	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-006	COI	45	BD44143	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.26127
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15707	WGS84	BD44145	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44145_Pper_Dia2013_COI	ESAV-008	1	BD44145_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-008	COI	45	BD44145	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-008	CTTAGTGGCATTAATTCGDAATCC	147.28853
BD44145	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15707	WGS84	BD44145	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	31.658	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44145_Vgib_Wil2025_COI	ESAV-008	0	BD44145_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44145	Sample	BD44145	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-008	COI	45	BD44145	1.00E-05	Nil	Australia	ESAV-008	CCCCTCTTTACAAGTTCTTTAGGGT	147.28853
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15595	WGS84	BD44146	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44146_Asen_Wil2025_COI	ESAV-009	0	BD44146_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-009	COI	45	BD44146	1.00E-05	Nil	Australia	ESAV-009	GTTGTTGTTACAACACATGCTCTTTTG	147.28775
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44146_Cjap_Sim2018_COI	ESAV-009	1	BD44146_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-009	COI	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.28775
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44146_Esin_And2018_Cyb	ESAV-009	1	BD44146_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-009	Cytb	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	ACCCCTCCTCATATCCAACCA	147.28775
BD44146	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15595	WGS84	BD44146	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44146_Mjap_Gib2022_COI	ESAV-009	1	BD44146_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44146	Sample	BD44146	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-009	COI	45	BD44146	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-009	AATTTAATTGTGACTAGGCATGGTCTG	147.28775
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44147_Hsan_Wil2021_COI	ESAV-010	1	BD44147_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-010	COI	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	CCTGGGCCGGTATAGTAGGT	147.28753
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	27.53	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44147_Mros_Gib2022_COI	ESAV-010	1	BD44147_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-010	COI	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28753
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44147_Pvir_Dia2013_COI	ESAV-010	1	BD44147_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-010	COI	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	CTTAGTGGCATTAATTCGDAATCC	147.28753
BD44147	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15468	WGS84	BD44147	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44147_Rhar_Sim2018_COI	ESAV-010	1	BD44147_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44147	Sample	BD44147	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-010	COI	45	BD44147	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-010	CTGCTGCTATTGCTCATG	147.28753
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15212	WGS84	BD44149	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44149_Pper_Dia2013_COI	ESAV-012	1	BD44149_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-012	COI	45	BD44149	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-012	CTTAGTGGCATTAATTCGDAATCC	147.28903
BD44149	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-43.15212	WGS84	BD44149	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	29.779	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD44149_Vgib_Wil2025_COI	ESAV-012	0	BD44149_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44149	Sample	BD44149	targeted	Present	Sample	Vgib_Wil2025_COI	ESAV-012	COI	45	BD44149	1.00E-05	Nil	Australia	ESAV-012	CCCCTCTTTACAAGTTCTTTAGGGT	147.28903
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44150_Hsan_Wil2021_COI	ESAV-013	1	BD44150_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-013	COI	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	CCTGGGCCGGTATAGTAGGT	147.28998
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	28.662	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44150_Mros_Gib2022_COI	ESAV-013	1	BD44150_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-013	COI	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.28998
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44150_Pvir_Dia2013_COI	ESAV-013	1	BD44150_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-013	COI	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	CTTAGTGGCATTAATTCGDAATCC	147.28998
BD44150	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15132	WGS84	BD44150	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44150_Rhar_Sim2018_COI	ESAV-013	1	BD44150_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44150	Sample	BD44150	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-013	COI	45	BD44150	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-013	CTGCTGCTATTGCTCATG	147.28998
BD44152	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-43.15357	WGS84	BD44152	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	12/08/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44152_Pper_Dia2013_COI	ESAV-015	1	BD44152_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44152	Sample	BD44152	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-015	COI	45	BD44152	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-015	CTTAGTGGCATTAATTCGDAATCC	147.28717
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44153_Cjap_Sim2018_COI	ESAV-016	1	BD44153_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-016	COI	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.88314
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44153_Esin_And2018_Cyb	ESAV-016	1	BD44153_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-016	Cytb	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	ACCCCTCCTCATATCCAACCA	147.88314
BD44153	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55457	WGS84	BD44153	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	36.409	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44153_Mjap_Gib2022_COI	ESAV-016	1	BD44153_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44153	Sample	BD44153	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-016	COI	45	BD44153	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-016	AATTTAATTGTGACTAGGCATGGTCTG	147.88314
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44154_Hsan_Wil2021_COI	ESAV-018	1	BD44154_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-018	COI	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	CCTGGGCCGGTATAGTAGGT	147.8818
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.646	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44154_Mros_Gib2022_COI	ESAV-018	1	BD44154_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-018	COI	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.8818
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44154_Pvir_Dia2013_COI	ESAV-018	1	BD44154_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-018	COI	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	CTTAGTGGCATTAATTCGDAATCC	147.8818
BD44154	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55424	WGS84	BD44154	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44154_Rhar_Sim2018_COI	ESAV-018	1	BD44154_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44154	Sample	BD44154	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-018	COI	45	BD44154	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-018	CTGCTGCTATTGCTCATG	147.8818
BD44156	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55399	WGS84	BD44156	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44156_Pper_Dia2013_COI	ESAV-020	1	BD44156_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44156	Sample	BD44156	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-020	COI	45	BD44156	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-020	CTTAGTGGCATTAATTCGDAATCC	147.87966
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44157_Cjap_Sim2018_COI	ESAV-022	1	BD44157_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-022	COI	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.8773
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44157_Esin_And2018_Cyb	ESAV-022	1	BD44157_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-022	Cytb	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	ACCCCTCCTCATATCCAACCA	147.8773
BD44157	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55517	WGS84	BD44157	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	31.052	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44157_Mjap_Gib2022_COI	ESAV-022	1	BD44157_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44157	Sample	BD44157	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-022	COI	45	BD44157	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-022	AATTTAATTGTGACTAGGCATGGTCTG	147.8773
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44158_Hsan_Wil2021_COI	ESAV-025	1	BD44158_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-025	COI	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	CCTGGGCCGGTATAGTAGGT	147.87623
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.531	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44158_Mros_Gib2022_COI	ESAV-025	1	BD44158_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-025	COI	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.87623
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44158_Pvir_Dia2013_COI	ESAV-025	1	BD44158_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-025	COI	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	CTTAGTGGCATTAATTCGDAATCC	147.87623
BD44158	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55601	WGS84	BD44158	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44158_Rhar_Sim2018_COI	ESAV-025	1	BD44158_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44158	Sample	BD44158	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-025	COI	45	BD44158	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-025	CTGCTGCTATTGCTCATG	147.87623
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44160_Cjap_Sim2018_COI	ESAV-030	1	BD44160_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-030	COI	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.87273
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44160_Esin_And2018_Cyb	ESAV-030	1	BD44160_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-030	Cytb	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	ACCCCTCCTCATATCCAACCA	147.87273
BD44160	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55772	WGS84	BD44160	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44160_Mjap_Gib2022_COI	ESAV-030	1	BD44160_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44160	Sample	BD44160	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-030	COI	45	BD44160	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-030	AATTTAATTGTGACTAGGCATGGTCTG	147.87273
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44161_Hsan_Wil2021_COI	ESAV-032	1	BD44161_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-032	COI	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	CCTGGGCCGGTATAGTAGGT	147.92961
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	30.554	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44161_Mros_Gib2022_COI	ESAV-032	1	BD44161_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-032	COI	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.92961
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44161_Pvir_Dia2013_COI	ESAV-032	1	BD44161_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-032	COI	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	CTTAGTGGCATTAATTCGDAATCC	147.92961
BD44161	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.54773	WGS84	BD44161	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44161_Rhar_Sim2018_COI	ESAV-032	1	BD44161_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44161	Sample	BD44161	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-032	COI	45	BD44161	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-032	CTGCTGCTATTGCTCATG	147.92961
BD44163	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51382	WGS84	BD44163	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44163_Pper_Dia2013_COI	ESAV-034	1	BD44163_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44163	Sample	BD44163	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-034	COI	45	BD44163	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-034	CTTAGTGGCATTAATTCGDAATCC	147.91372
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44164_Cjap_Sim2018_COI	ESAV-035	1	BD44164_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-035	COI	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	147.9142
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44164_Esin_And2018_Cyb	ESAV-035	1	BD44164_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-035	Cytb	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	ACCCCTCCTCATATCCAACCA	147.9142
BD44164	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51328	WGS84	BD44164	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	32.651	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44164_Mjap_Gib2022_COI	ESAV-035	1	BD44164_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44164	Sample	BD44164	targeted	Present	Sample	Mjap_Gib2022_COI	ESAV-035	COI	45	BD44164	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-035	AATTTAATTGTGACTAGGCATGGTCTG	147.9142
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44165_Hsan_Wil2021_COI	ESAV-036	1	BD44165_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-036	COI	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	CCTGGGCCGGTATAGTAGGT	147.91501
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	29.658	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44165_Mros_Gib2022_COI	ESAV-036	1	BD44165_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Present	Sample	Mros_Gib2022_COI	ESAV-036	COI	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	ACTTCTACCTCCTGCTCTTCTACTTCTC	147.91501
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44165_Pvir_Dia2013_COI	ESAV-036	1	BD44165_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-036	COI	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	CTTAGTGGCATTAATTCGDAATCC	147.91501
BD44165	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.51095	WGS84	BD44165	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44165_Rhar_Sim2018_COI	ESAV-036	1	BD44165_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44165	Sample	BD44165	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-036	COI	45	BD44165	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-036	CTGCTGCTATTGCTCATG	147.91501
BD44167	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55275	WGS84	BD44167	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44167_Pper_Dia2013_COI	ESAV-038	1	BD44167_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44167	Sample	BD44167	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-038	COI	45	BD44167	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-038	CTTAGTGGCATTAATTCGDAATCC	147.91067
BD44168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16578	-42.55335	WGS84	BD44168	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Not assessed	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD44168_Asen_Wil2025_COI	ESAV-039	0	BD44168_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44168	Sample	BD44168	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-039	COI	45	BD44168	1.00E-05	Nil	Australia	ESAV-039	GTTGTTGTTACAACACATGCTCTTTTG	147.89548
BD44168	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14680	-42.55335	WGS84	BD44168	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Giblot-Ducray D, Herdina, Ellard K, Deveney MR, Wiltshire KH (2022) Development of TaqMan MGB quantitative PCR assays for the invasive species Maoricolpus roseus (Quoy & Gaimard, 1834) and Mya japonica (Jay, 1857). Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000222-1. SARDI Research Report Series No. 1149., Adelaide.	2021_Assay_validation_sampling-Tas_Mya_and_Maoricolpus_Spring	16/12/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44168_Mjap_Gib2022_COI	ESAV-039	1	BD44168_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44168	Sample	BD44168	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-039	COI	45	BD44168	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-039	AATTTAATTGTGACTAGGCATGGTCTG	147.89548
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44708_Cjap_Sim2018_COI	POR-B23	1	BD44708_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44708_Esin_And2018_Cyb	POR-B23	1	BD44708_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B23	Cytb	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	ACCCCTCCTCATATCCAACCA	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD44708_Mgig_Bot2012_COI	POR-B23	1	BD44708_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	TCTTATTCGTTGGAGACTTTATAACCCT	141.61277
BD44708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3469	WGS84	BD44708	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44708_Mjap_Gib2022_COI	POR-B23	1	BD44708_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44708	Sample	BD44708	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B23	COI	45	BD44708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B23	AATTTAATTGTGACTAGGCATGGTCTG	141.61277
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD44709_Cmae_Bot2011_COI	POR-B24	1	BD44709_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	ATGAACAGTCTATCCTCCTTTAG	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44709_Hsan_Wil2021_COI	POR-B24	1	BD44709_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	CCTGGGCCGGTATAGTAGGT	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44709_Mros_Gib2022_COI	POR-B24	1	BD44709_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Mros_Gib2022_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD44709_Msal_Bot2012_COI	POR-B24	1	BD44709_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	GYTAGTTCCRATGATGTTAGCTG	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD44709_Mstr_Wil2021_COI	POR-B24	1	BD44709_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	GTAGTGGTAACTACTCATGCTTTA	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44709_Pvir_Dia2013_COI	POR-B24	1	BD44709_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	CTTAGTGGCATTAATTCGDAATCC	141.61416
BD44709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34679	WGS84	BD44709	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44709_Rhar_Sim2018_COI	POR-B24	1	BD44709_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44709	Sample	BD44709	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B24	COI	45	BD44709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B24	CTGCTGCTATTGCTCATG	141.61416
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD44710_Aamu_Bax2006_COI	POR-B25	1	BD44710_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	GCACAACCGGGATCTTTACTTC	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD44710_Pcan_Bot2011_IGS	POR-B25	1	BD44710_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B25	IGS	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	CGTAATCCTCAGTACTGGCTA	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44710_Pper_Dia2013_COI	POR-B25	1	BD44710_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	CTTAGTGGCATTAATTCGDAATCC	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD44710_Sspa_Oph2007_COI	POR-B25	1	BD44710_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	GAAGGCCTTCCGATGATC	141.61558
BD44710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34675	WGS84	BD44710	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	32.828	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD44710_Upin_Bot2011_COI	POR-B25	1	BD44710_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44710	Sample	BD44710	targeted	Present	Sample	Upin_Bot2011_COI	POR-B25	COI	45	BD44710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B25	CTTTAATTACAGCGTTTTTATTGTTGT	141.61558
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD44711_Cjap_Sim2018_COI	POR-B26	1	BD44711_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Cjap_Sim2018_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	TTAATATACGGTCATTTGGTATGAGTATAGATC	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD44711_Esin_And2018_Cyb	POR-B26	1	BD44711_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Esin_And2018_Cyb	POR-B26	Cytb	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	ACCCCTCCTCATATCCAACCA	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BD44711_Mgig_Bot2012_COI	POR-B26	1	BD44711_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Mgig_Bot2012_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	TCTTATTCGTTGGAGACTTTATAACCCT	141.61693
BD44711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34668	WGS84	BD44711	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD44711_Mjap_Gib2022_COI	POR-B26	1	BD44711_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44711	Sample	BD44711	targeted	Absent	Sample	Mjap_Gib2022_COI	POR-B26	COI	45	BD44711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B26	AATTTAATTGTGACTAGGCATGGTCTG	141.61693
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BD44712_Cmae_Bot2011_COI	POR-B27	1	BD44712_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Cmae_Bot2011_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	ATGAACAGTCTATCCTCCTTTAG	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD44712_Hsan_Wil2021_COI	POR-B27	1	BD44712_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Hsan_Wil2021_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	CCTGGGCCGGTATAGTAGGT	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	32.883	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD44712_Mros_Gib2022_COI	POR-B27	1	BD44712_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Present	Sample	Mros_Gib2022_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	ACTTCTACCTCCTGCTCTTCTACTTCTC	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD44712_Msal_Bot2012_COI	POR-B27	1	BD44712_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Msal_Bot2012_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	GYTAGTTCCRATGATGTTAGCTG	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BD44712_Mstr_Wil2021_COI	POR-B27	1	BD44712_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Mstr_Wil2021_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	GTAGTGGTAACTACTCATGCTTTA	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD44712_Pvir_Dia2013_COI	POR-B27	1	BD44712_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Pvir_Dia2013_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	CTTAGTGGCATTAATTCGDAATCC	141.61831
BD44712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.3466	WGS84	BD44712	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD44712_Rhar_Sim2018_COI	POR-B27	1	BD44712_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44712	Sample	BD44712	targeted	Absent	Sample	Rhar_Sim2018_COI	POR-B27	COI	45	BD44712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B27	CTGCTGCTATTGCTCATG	141.61831
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BD44714_Aamu_Bax2006_COI	POR-B35	1	BD44714_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Aamu_Bax2006_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	GCACAACCGGGATCTTTACTTC	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD44714_Pcan_Bot2011_IGS	POR-B35	1	BD44714_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Pcan_Bot2011_IGS	POR-B35	IGS	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	CGTAATCCTCAGTACTGGCTA	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD44714_Pper_Dia2013_COI	POR-B35	1	BD44714_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Pper_Dia2013_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	CTTAGTGGCATTAATTCGDAATCC	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BD44714_Sspa_Oph2007_COI	POR-B35	1	BD44714_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Sspa_Oph2007_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	GAAGGCCTTCCGATGATC	141.61984
BD44714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14710	-38.34845	WGS84	BD44714	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Deveney MR (2022) Molecular surveys for priority marine pests in 2021-22 for Thevenard, Port Lincoln, Port Giles, Klein Point, Port Adelaide, South Australia, and Portland, Victoria.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2022/000236-1. SARDI Research Report Series No. 1147., Adelaide.	2021-22_Molecular_surveys-Portland_Summer	27/03/2022	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BD44714_Upin_Bot2011_COI	POR-B35	1	BD44714_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD44714	Sample	BD44714	targeted	Absent	Sample	Upin_Bot2011_COI	POR-B35	COI	45	BD44714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	POR-B35	CTTTAATTACAGCGTTTTTATTGTTGT	141.61984
BD47585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.96444	WGS84	BD47585	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47585_Esin_And2018_Cyb	ESAV-236	1	BD47585_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47585	Sample	BD47585	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-236	Cytb	45	BD47585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-236	ACCCCTCCTCATATCCAACCA	137.77685
BD47585	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.96444	WGS84	BD47585	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Summer	3/03/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47585_Mjap_Gib2022_COI	ESAV-236	1	BD47585_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47585	Sample	BD47585	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-236	COI	45	BD47585	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-236	AATTTAATTGTGACTAGGCATGGTCTG	137.77685
BD47589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85119	WGS84	BD47589	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47589_Esin_And2018_Cyb	ESAV-096	1	BD47589_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47589	Sample	BD47589	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-096	Cytb	45	BD47589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-096	ACCCCTCCTCATATCCAACCA	138.46606
BD47589	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85119	WGS84	BD47589	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47589_Mjap_Gib2022_COI	ESAV-096	1	BD47589_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47589	Sample	BD47589	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-096	COI	45	BD47589	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-096	AATTTAATTGTGACTAGGCATGGTCTG	138.46606
BD47592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8198	WGS84	BD47592	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47592_Asen_Wil2025_COI	GC30	1	BD47592_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47592	Sample	BD47592	targeted	Absent	Sample	Asen_Wil2025_COI	GC30	COI	45	BD47592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC30	GTTGTTGTTACAACACATGCTCTTTTG	151.24001
BD47592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8198	WGS84	BD47592	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47592_Esin_And2018_Cyb	GC30	1	BD47592_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47592	Sample	BD47592	targeted	Absent	Sample	Esin_And2018_Cyb	GC30	Cytb	45	BD47592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC30	ACCCCTCCTCATATCCAACCA	151.24001
BD47592	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8198	WGS84	BD47592	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47592_Mjap_Gib2022_COI	GC30	1	BD47592_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47592	Sample	BD47592	targeted	Absent	Sample	Mjap_Gib2022_COI	GC30	COI	45	BD47592	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC30	AATTTAATTGTGACTAGGCATGGTCTG	151.24001
BD47596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86518	WGS84	BD47596	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47596_Esin_And2018_Cyb	ESAV-110	1	BD47596_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47596	Sample	BD47596	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-110	Cytb	45	BD47596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-110	ACCCCTCCTCATATCCAACCA	138.46542
BD47596	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.86518	WGS84	BD47596	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47596_Mjap_Gib2022_COI	ESAV-110	1	BD47596_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47596	Sample	BD47596	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-110	COI	45	BD47596	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-110	AATTTAATTGTGACTAGGCATGGTCTG	138.46542
BD47600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82069	WGS84	BD47600	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47600_Esin_And2018_Cyb	GB100	1	BD47600_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47600	Sample	BD47600	targeted	Absent	Sample	Esin_And2018_Cyb	GB100	Cytb	45	BD47600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB100	ACCCCTCCTCATATCCAACCA	151.24147
BD47600	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82069	WGS84	BD47600	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47600_Mjap_Gib2022_COI	GB100	1	BD47600_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47600	Sample	BD47600	targeted	Absent	Sample	Mjap_Gib2022_COI	GB100	COI	45	BD47600	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB100	AATTTAATTGTGACTAGGCATGGTCTG	151.24147
BD47604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82408	WGS84	BD47604	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47604_Esin_And2018_Cyb	ESAV-155	1	BD47604_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47604	Sample	BD47604	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-155	Cytb	45	BD47604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-155	ACCCCTCCTCATATCCAACCA	138.48026
BD47604	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82408	WGS84	BD47604	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47604_Mjap_Gib2022_COI	ESAV-155	1	BD47604_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47604	Sample	BD47604	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-155	COI	45	BD47604	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-155	AATTTAATTGTGACTAGGCATGGTCTG	138.48026
BD47608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78742	WGS84	BD47608	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47608_Esin_And2018_Cyb	GC89	1	BD47608_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47608	Sample	BD47608	targeted	Absent	Sample	Esin_And2018_Cyb	GC89	Cytb	45	BD47608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC89	ACCCCTCCTCATATCCAACCA	151.17475
BD47608	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78742	WGS84	BD47608	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47608_Mjap_Gib2022_COI	GC89	1	BD47608_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47608	Sample	BD47608	targeted	Absent	Sample	Mjap_Gib2022_COI	GC89	COI	45	BD47608	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC89	AATTTAATTGTGACTAGGCATGGTCTG	151.17475
BD47611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.79115	WGS84	BD47611	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47611_Esin_And2018_Cyb	GC99	1	BD47611_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47611	Sample	BD47611	targeted	Absent	Sample	Esin_And2018_Cyb	GC99	Cytb	45	BD47611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC99	ACCCCTCCTCATATCCAACCA	151.17999
BD47611	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.79115	WGS84	BD47611	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47611_Mjap_Gib2022_COI	GC99	1	BD47611_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47611	Sample	BD47611	targeted	Absent	Sample	Mjap_Gib2022_COI	GC99	COI	45	BD47611	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC99	AATTTAATTGTGACTAGGCATGGTCTG	151.17999
BD47615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01932	WGS84	BD47615	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47615_Esin_And2018_Cyb	ESAV-234	1	BD47615_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47615	Sample	BD47615	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-234	Cytb	45	BD47615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-234	ACCCCTCCTCATATCCAACCA	137.7696
BD47615	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01932	WGS84	BD47615	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47615_Mjap_Gib2022_COI	ESAV-234	1	BD47615_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47615	Sample	BD47615	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-234	COI	45	BD47615	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-234	AATTTAATTGTGACTAGGCATGGTCTG	137.7696
BD47619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85579	WGS84	BD47619	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47619_Esin_And2018_Cyb	ESAV-100	1	BD47619_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47619	Sample	BD47619	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-100	Cytb	45	BD47619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-100	ACCCCTCCTCATATCCAACCA	138.46577
BD47619	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85579	WGS84	BD47619	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47619_Mjap_Gib2022_COI	ESAV-100	1	BD47619_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47619	Sample	BD47619	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-100	COI	45	BD47619	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-100	AATTTAATTGTGACTAGGCATGGTCTG	138.46577
BD47622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83492	WGS84	BD47622	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47622_Esin_And2018_Cyb	GB19	1	BD47622_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47622	Sample	BD47622	targeted	Absent	Sample	Esin_And2018_Cyb	GB19	Cytb	45	BD47622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB19	ACCCCTCCTCATATCCAACCA	151.27091
BD47622	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83492	WGS84	BD47622	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47622_Mjap_Gib2022_COI	GB19	1	BD47622_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47622	Sample	BD47622	targeted	Absent	Sample	Mjap_Gib2022_COI	GB19	COI	45	BD47622	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB19	AATTTAATTGTGACTAGGCATGGTCTG	151.27091
BD47623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.82193	WGS84	BD47623	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	30/07/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47623_Msal_Bot2012_COI	GA21	1	BD47623_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47623	Sample	BD47623	targeted	Absent	Sample	Msal_Bot2012_COI	GA21	COI	45	BD47623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA21	GYTAGTTCCRATGATGTTAGCTG	151.24014
BD47625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85025	WGS84	BD47625	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47625_Pcan_Bot2011_IGS	ESAV-134	1	BD47625_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47625	Sample	BD47625	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-134	IGS	45	BD47625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-134	CGTAATCCTCAGTACTGGCTA	138.4712
BD47625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85025	WGS84	BD47625	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47625_Pper_Dia2013_COI	ESAV-134	1	BD47625_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47625	Sample	BD47625	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-134	COI	45	BD47625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-134	CTTAGTGGCATTAATTCGDAATCC	138.4712
BD47625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85025	WGS84	BD47625	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47625_Vgib_Wil2025_COI	ESAV-134	1	BD47625_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47625	Sample	BD47625	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-134	COI	45	BD47625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-134	CCCCTCTTTACAAGTTCTTTAGGGT	138.4712
BD47626	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85478	WGS84	BD47626	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47626_Esin_And2018_Cyb	ESAV-131	1	BD47626_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47626	Sample	BD47626	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-131	Cytb	45	BD47626	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-131	ACCCCTCCTCATATCCAACCA	138.47153
BD47629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83771	WGS84	BD47629	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47629_Pcan_Bot2011_IGS	ESAV-054	1	BD47629_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47629	Sample	BD47629	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-054	IGS	45	BD47629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-054	CGTAATCCTCAGTACTGGCTA	138.46302
BD47629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83771	WGS84	BD47629	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47629_Pper_Dia2013_COI	ESAV-054	1	BD47629_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47629	Sample	BD47629	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-054	COI	45	BD47629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-054	CTTAGTGGCATTAATTCGDAATCC	138.46302
BD47629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83771	WGS84	BD47629	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47629_Vgib_Wil2025_COI	ESAV-054	1	BD47629_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47629	Sample	BD47629	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-054	COI	45	BD47629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-054	CCCCTCTTTACAAGTTCTTTAGGGT	138.46302
BD47632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8061	WGS84	BD47632	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47632_Pcan_Bot2011_IGS	GB36	1	BD47632_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47632	Sample	BD47632	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB36	IGS	45	BD47632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB36	CGTAATCCTCAGTACTGGCTA	151.21873
BD47632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.8061	WGS84	BD47632	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47632_Pper_Dia2013_COI	GB36	1	BD47632_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47632	Sample	BD47632	targeted	Absent	Sample	Pper_Dia2013_COI	GB36	COI	45	BD47632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB36	CTTAGTGGCATTAATTCGDAATCC	151.21873
BD47636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81849	WGS84	BD47636	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47636_Pcan_Bot2011_IGS	ESAV-161	1	BD47636_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47636	Sample	BD47636	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-161	IGS	45	BD47636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-161	CGTAATCCTCAGTACTGGCTA	138.48251
BD47636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81849	WGS84	BD47636	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47636_Pper_Dia2013_COI	ESAV-161	1	BD47636_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47636	Sample	BD47636	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-161	COI	45	BD47636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-161	CTTAGTGGCATTAATTCGDAATCC	138.48251
BD47636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.81849	WGS84	BD47636	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47636_Vgib_Wil2025_COI	ESAV-161	1	BD47636_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47636	Sample	BD47636	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-161	COI	45	BD47636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-161	CCCCTCTTTACAAGTTCTTTAGGGT	138.48251
BD47637	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.85887	WGS84	BD47637	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47637_Esin_And2018_Cyb	ESAV-127	1	BD47637_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47637	Sample	BD47637	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-127	Cytb	45	BD47637	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-127	ACCCCTCCTCATATCCAACCA	138.47
BD47643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84129	WGS84	BD47643	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47643_Pcan_Bot2011_IGS	ESAV-050	1	BD47643_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47643	Sample	BD47643	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-050	IGS	45	BD47643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-050	CGTAATCCTCAGTACTGGCTA	138.46358
BD47643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84129	WGS84	BD47643	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47643_Pper_Dia2013_COI	ESAV-050	1	BD47643_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47643	Sample	BD47643	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-050	COI	45	BD47643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-050	CTTAGTGGCATTAATTCGDAATCC	138.46358
BD47643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84129	WGS84	BD47643	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47643_Vgib_Wil2025_COI	ESAV-050	1	BD47643_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47643	Sample	BD47643	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-050	COI	45	BD47643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-050	CCCCTCTTTACAAGTTCTTTAGGGT	138.46358
BD47647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83858	WGS84	BD47647	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47647_Pcan_Bot2011_IGS	ESAV-053	1	BD47647_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47647	Sample	BD47647	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-053	IGS	45	BD47647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-053	CGTAATCCTCAGTACTGGCTA	138.4633
BD47647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83858	WGS84	BD47647	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47647_Pper_Dia2013_COI	ESAV-053	1	BD47647_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47647	Sample	BD47647	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-053	COI	45	BD47647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-053	CTTAGTGGCATTAATTCGDAATCC	138.4633
BD47647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.83858	WGS84	BD47647	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47647_Vgib_Wil2025_COI	ESAV-053	1	BD47647_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47647	Sample	BD47647	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-053	COI	45	BD47647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-053	CCCCTCTTTACAAGTTCTTTAGGGT	138.4633
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47648_Asen_Wil2025_COI	ESAV-046	1	BD47648_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-046	COI	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	GTTGTTGTTACAACACATGCTCTTTTG	138.46418
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47648_Cjap_Sim2018_COI	ESAV-046	1	BD47648_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-046	COI	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46418
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47648_Esin_And2018_Cyb	ESAV-046	1	BD47648_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Esin_And2018_Cyb	ESAV-046	Cytb	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	ACCCCTCCTCATATCCAACCA	138.46418
BD47648	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.84505	WGS84	BD47648	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47648_Mjap_Gib2022_COI	ESAV-046	1	BD47648_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47648	Sample	BD47648	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-046	COI	45	BD47648	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-046	AATTTAATTGTGACTAGGCATGGTCTG	138.46418
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47649_Hsan_Wil2021_COI	GC22	1	BD47649_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Hsan_Wil2021_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	CCTGGGCCGGTATAGTAGGT	151.25658
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47649_Msal_Bot2012_COI	GC22	1	BD47649_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Msal_Bot2012_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	GYTAGTTCCRATGATGTTAGCTG	151.25658
BD47649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83127	WGS84	BD47649	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47649_Rhar_Sim2018_COI	GC22	1	BD47649_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47649	Sample	BD47649	targeted	Absent	Sample	Rhar_Sim2018_COI	GC22	COI	45	BD47649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC22	CTGCTGCTATTGCTCATG	151.25658
BD47650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81559	WGS84	BD47650	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47650_Pcan_Bot2011_IGS	GC67	1	BD47650_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47650	Sample	BD47650	targeted	Absent	Sample	Pcan_Bot2011_IGS	GC67	IGS	45	BD47650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC67	CGTAATCCTCAGTACTGGCTA	151.23557
BD47650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81559	WGS84	BD47650	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47650_Pper_Dia2013_COI	GC67	1	BD47650_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47650	Sample	BD47650	targeted	Absent	Sample	Pper_Dia2013_COI	GC67	COI	45	BD47650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC67	CTTAGTGGCATTAATTCGDAATCC	151.23557
BD47650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.81559	WGS84	BD47650	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47650_Vgib_Wil2025_COI	GC67	1	BD47650_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47650	Sample	BD47650	targeted	Absent	Sample	Vgib_Wil2025_COI	GC67	COI	45	BD47650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC67	CCCCTCTTTACAAGTTCTTTAGGGT	151.23557
BD47654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01847	WGS84	BD47654	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47654_Pcan_Bot2011_IGS	ESAV-235	1	BD47654_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47654	Sample	BD47654	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-235	IGS	45	BD47654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-235	CGTAATCCTCAGTACTGGCTA	137.7687
BD47654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01847	WGS84	BD47654	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47654_Pper_Dia2013_COI	ESAV-235	1	BD47654_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47654	Sample	BD47654	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-235	COI	45	BD47654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-235	CTTAGTGGCATTAATTCGDAATCC	137.7687
BD47654	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-35.01847	WGS84	BD47654	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47654_Vgib_Wil2025_COI	ESAV-235	1	BD47654_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47654	Sample	BD47654	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-235	COI	45	BD47654	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-235	CCCCTCTTTACAAGTTCTTTAGGGT	137.7687
BD47658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82125	WGS84	BD47658	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47658_Pcan_Bot2011_IGS	ESAV-158	1	BD47658_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47658	Sample	BD47658	targeted	Absent	Sample	Pcan_Bot2011_IGS	ESAV-158	IGS	45	BD47658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-158	CGTAATCCTCAGTACTGGCTA	138.48193
BD47658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82125	WGS84	BD47658	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47658_Pper_Dia2013_COI	ESAV-158	1	BD47658_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47658	Sample	BD47658	targeted	Absent	Sample	Pper_Dia2013_COI	ESAV-158	COI	45	BD47658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-158	CTTAGTGGCATTAATTCGDAATCC	138.48193
BD47658	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82125	WGS84	BD47658	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47658_Vgib_Wil2025_COI	ESAV-158	1	BD47658_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47658	Sample	BD47658	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-158	COI	45	BD47658	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-158	CCCCTCTTTACAAGTTCTTTAGGGT	138.48193
BD47659	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-34.82743	WGS84	BD47659	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47659_Mjap_Gib2022_COI	ESAV-151	1	BD47659_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47659	Sample	BD47659	targeted	Absent	Sample	Mjap_Gib2022_COI	ESAV-151	COI	45	BD47659	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-151	AATTTAATTGTGACTAGGCATGGTCTG	138.47671
BD47661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78689	WGS84	BD47661	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47661_Pcan_Bot2011_IGS	GB49	1	BD47661_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47661	Sample	BD47661	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB49	IGS	45	BD47661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB49	CGTAATCCTCAGTACTGGCTA	151.17419
BD47661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78689	WGS84	BD47661	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BD47661_Pper_Dia2013_COI	GB49	1	BD47661_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47661	Sample	BD47661	targeted	Absent	Sample	Pper_Dia2013_COI	GB49	COI	45	BD47661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB49	CTTAGTGGCATTAATTCGDAATCC	151.17419
BD47661	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.78689	WGS84	BD47661	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47661_Vgib_Wil2025_COI	GB49	1	BD47661_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47661	Sample	BD47661	targeted	Absent	Sample	Vgib_Wil2025_COI	GB49	COI	45	BD47661	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB49	CCCCTCTTTACAAGTTCTTTAGGGT	151.17419
BD47662	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14740	-23.83533	WGS84	BD47662	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BD47662_Esin_And2018_Cyb	GB17	1	BD47662_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47662	Sample	BD47662	targeted	Absent	Sample	Esin_And2018_Cyb	GB17	Cytb	45	BD47662	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB17	ACCCCTCCTCATATCCAACCA	151.27433
BD47667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80933	WGS84	BD47667	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47667_Hsan_Wil2021_COI	ESAV-085	1	BD47667_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47667	Sample	BD47667	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-085	COI	45	BD47667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-085	CCTGGGCCGGTATAGTAGGT	138.4643
BD47667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80933	WGS84	BD47667	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47667_Msal_Bot2012_COI	ESAV-085	1	BD47667_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47667	Sample	BD47667	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-085	COI	45	BD47667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-085	GYTAGTTCCRATGATGTTAGCTG	138.4643
BD47667	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80933	WGS84	BD47667	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47667_Pvir_Dia2013_COI	ESAV-085	1	BD47667_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47667	Sample	BD47667	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-085	COI	45	BD47667	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-085	CTTAGTGGCATTAATTCGDAATCC	138.4643
BD47674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82227	WGS84	BD47674	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47674_Hsan_Wil2021_COI	ESAV-157	1	BD47674_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47674	Sample	BD47674	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-157	COI	45	BD47674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-157	CCTGGGCCGGTATAGTAGGT	138.48171
BD47674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82227	WGS84	BD47674	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47674_Msal_Bot2012_COI	ESAV-157	1	BD47674_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47674	Sample	BD47674	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-157	COI	45	BD47674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-157	GYTAGTTCCRATGATGTTAGCTG	138.48171
BD47674	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82227	WGS84	BD47674	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47674_Pvir_Dia2013_COI	ESAV-157	1	BD47674_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47674	Sample	BD47674	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-157	COI	45	BD47674	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-157	CTTAGTGGCATTAATTCGDAATCC	138.48171
BD47678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80476	WGS84	BD47678	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47678_Hsan_Wil2021_COI	ESAV-090	1	BD47678_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47678	Sample	BD47678	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-090	COI	45	BD47678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-090	CCTGGGCCGGTATAGTAGGT	138.46491
BD47678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80476	WGS84	BD47678	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47678_Msal_Bot2012_COI	ESAV-090	1	BD47678_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47678	Sample	BD47678	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-090	COI	45	BD47678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-090	GYTAGTTCCRATGATGTTAGCTG	138.46491
BD47678	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80476	WGS84	BD47678	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47678_Pvir_Dia2013_COI	ESAV-090	1	BD47678_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47678	Sample	BD47678	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-090	COI	45	BD47678	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-090	CTTAGTGGCATTAATTCGDAATCC	138.46491
BD47681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82023	WGS84	BD47681	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47681_Hsan_Wil2021_COI	MA80	1	BD47681_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47681	Sample	BD47681	targeted	Absent	Sample	Hsan_Wil2021_COI	MA80	COI	45	BD47681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA80	CCTGGGCCGGTATAGTAGGT	151.2409
BD47681	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82023	WGS84	BD47681	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47681_Msal_Bot2012_COI	MA80	1	BD47681_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47681	Sample	BD47681	targeted	Absent	Sample	Msal_Bot2012_COI	MA80	COI	45	BD47681	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA80	GYTAGTTCCRATGATGTTAGCTG	151.2409
BD47685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-35.01856	WGS84	BD47685	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47685_Hsan_Wil2021_COI	ESAV-231	1	BD47685_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47685	Sample	BD47685	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-231	COI	45	BD47685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-231	CCTGGGCCGGTATAGTAGGT	137.76584
BD47685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-35.01856	WGS84	BD47685	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47685_Msal_Bot2012_COI	ESAV-231	1	BD47685_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47685	Sample	BD47685	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-231	COI	45	BD47685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-231	GYTAGTTCCRATGATGTTAGCTG	137.76584
BD47685	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-35.01856	WGS84	BD47685	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Giles_Summer	3/02/2022	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47685_Pvir_Dia2013_COI	ESAV-231	1	BD47685_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47685	Sample	BD47685	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-231	COI	45	BD47685	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-231	CTTAGTGGCATTAATTCGDAATCC	137.76584
BD47689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81205	WGS84	BD47689	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47689_Hsan_Wil2021_COI	ESAV-077	1	BD47689_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47689	Sample	BD47689	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-077	COI	45	BD47689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-077	CCTGGGCCGGTATAGTAGGT	138.46397
BD47689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81205	WGS84	BD47689	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47689_Msal_Bot2012_COI	ESAV-077	1	BD47689_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47689	Sample	BD47689	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-077	COI	45	BD47689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-077	GYTAGTTCCRATGATGTTAGCTG	138.46397
BD47689	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81205	WGS84	BD47689	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47689_Pvir_Dia2013_COI	ESAV-077	1	BD47689_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47689	Sample	BD47689	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-077	COI	45	BD47689	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-077	CTTAGTGGCATTAATTCGDAATCC	138.46397
BD47696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82028	WGS84	BD47696	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47696_Hsan_Wil2021_COI	ESAV-159	1	BD47696_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47696	Sample	BD47696	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-159	COI	45	BD47696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-159	CCTGGGCCGGTATAGTAGGT	138.48196
BD47696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82028	WGS84	BD47696	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47696_Msal_Bot2012_COI	ESAV-159	1	BD47696_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47696	Sample	BD47696	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-159	COI	45	BD47696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-159	GYTAGTTCCRATGATGTTAGCTG	138.48196
BD47696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82028	WGS84	BD47696	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47696_Pvir_Dia2013_COI	ESAV-159	1	BD47696_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47696	Sample	BD47696	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-159	COI	45	BD47696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-159	CTTAGTGGCATTAATTCGDAATCC	138.48196
BD47700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83225	WGS84	BD47700	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47700_Hsan_Wil2021_COI	ESAV-060	1	BD47700_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47700	Sample	BD47700	targeted	Absent	Sample	Hsan_Wil2021_COI	ESAV-060	COI	45	BD47700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-060	CCTGGGCCGGTATAGTAGGT	138.46248
BD47700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83225	WGS84	BD47700	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47700_Msal_Bot2012_COI	ESAV-060	1	BD47700_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47700	Sample	BD47700	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-060	COI	45	BD47700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-060	GYTAGTTCCRATGATGTTAGCTG	138.46248
BD47700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83225	WGS84	BD47700	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47700_Pvir_Dia2013_COI	ESAV-060	1	BD47700_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47700	Sample	BD47700	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-060	COI	45	BD47700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-060	CTTAGTGGCATTAATTCGDAATCC	138.46248
BD47708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.78913	WGS84	BD47708	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47708_Rhar_Sim2018_COI	GC91	1	BD47708_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47708	Sample	BD47708	targeted	Absent	Sample	Rhar_Sim2018_COI	GC91	COI	45	BD47708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC91	CTGCTGCTATTGCTCATG	151.17637
BD47710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84682	WGS84	BD47710	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47710_Asen_Wil2025_COI	ESAV-044	1	BD47710_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47710	Sample	BD47710	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-044	COI	45	BD47710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-044	GTTGTTGTTACAACACATGCTCTTTTG	138.46405
BD47711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82291	WGS84	BD47711	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47711_Rhar_Sim2018_COI	GC41	1	BD47711_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47711	Sample	BD47711	targeted	Absent	Sample	Rhar_Sim2018_COI	GC41	COI	45	BD47711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC41	CTGCTGCTATTGCTCATG	151.24344
BD47714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.80039	WGS84	BD47714	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47714_Asen_Wil2025_COI	ESAV-178	1	BD47714_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47714	Sample	BD47714	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-178	COI	45	BD47714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-178	GTTGTTGTTACAACACATGCTCTTTTG	138.48192
BD47715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84269	WGS84	BD47715	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47715_Rhar_Sim2018_COI	ESAV-140	1	BD47715_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47715	Sample	BD47715	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-140	COI	45	BD47715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-140	CTGCTGCTATTGCTCATG	138.47128
BD47718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82857	WGS84	BD47718	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47718_Asen_Wil2025_COI	ESAV-064	1	BD47718_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47718	Sample	BD47718	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-064	COI	45	BD47718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-064	GTTGTTGTTACAACACATGCTCTTTTG	138.46228
BD47719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83945	WGS84	BD47719	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47719_Msal_Bot2012_COI	ESAV-052	1	BD47719_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47719	Sample	BD47719	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-052	COI	45	BD47719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-052	GYTAGTTCCRATGATGTTAGCTG	138.46355
BD47719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.83945	WGS84	BD47719	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47719_Rhar_Sim2018_COI	ESAV-052	1	BD47719_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47719	Sample	BD47719	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-052	COI	45	BD47719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-052	CTGCTGCTATTGCTCATG	138.46355
BD47721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.83703	WGS84	BD47721	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47721_Asen_Wil2025_COI	GC15	1	BD47721_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47721	Sample	BD47721	targeted	Absent	Sample	Asen_Wil2025_COI	GC15	COI	45	BD47721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC15	GTTGTTGTTACAACACATGCTCTTTTG	151.27364
BD47722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79309	WGS84	BD47722	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47722_Msal_Bot2012_COI	GC97	1	BD47722_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47722	Sample	BD47722	targeted	Absent	Sample	Msal_Bot2012_COI	GC97	COI	45	BD47722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC97	GYTAGTTCCRATGATGTTAGCTG	151.18256
BD47725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.82037	WGS84	BD47725	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47725_Asen_Wil2025_COI	ESAV-073	1	BD47725_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47725	Sample	BD47725	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-073	COI	45	BD47725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-073	GTTGTTGTTACAACACATGCTCTTTTG	138.4632
BD47728	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.7899	WGS84	BD47728	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	3/01/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47728_Vgib_Wil2025_COI	GC93	1	BD47728_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47728	Sample	BD47728	targeted	Absent	Sample	Vgib_Wil2025_COI	GC93	COI	45	BD47728	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC93	CCCCTCTTTACAAGTTCTTTAGGGT	151.17787
BD47729	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.84391	WGS84	BD47729	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47729_Asen_Wil2025_COI	ESAV-091	1	BD47729_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47729	Sample	BD47729	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-091	COI	45	BD47729	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-091	GTTGTTGTTACAACACATGCTCTTTTG	138.46741
BD47731	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.85136	WGS84	BD47731	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47731_Vgib_Wil2025_COI	GC4	1	BD47731_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47731	Sample	BD47731	targeted	Absent	Sample	Vgib_Wil2025_COI	GC4	COI	45	BD47731	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC4	CCCCTCTTTACAAGTTCTTTAGGGT	151.31385
BD47732	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82044	WGS84	BD47732	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Autumn	26/06/2018	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47732_Asen_Wil2025_COI	GD13	1	BD47732_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47732	Sample	BD47732	targeted	Absent	Sample	Asen_Wil2025_COI	GD13	COI	45	BD47732	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GD13	GTTGTTGTTACAACACATGCTCTTTTG	151.24022
BD47733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.78577	WGS84	BD47733	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/01/2017	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47733_Rhar_Sim2018_COI	GA59	1	BD47733_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47733	Sample	BD47733	targeted	Absent	Sample	Rhar_Sim2018_COI	GA59	COI	45	BD47733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA59	CTGCTGCTATTGCTCATG	151.17282
BD47736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.85575	WGS84	BD47736	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47736_Asen_Wil2025_COI	ESAV-130	1	BD47736_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47736	Sample	BD47736	targeted	Absent	Sample	Asen_Wil2025_COI	ESAV-130	COI	45	BD47736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-130	GTTGTTGTTACAACACATGCTCTTTTG	138.47129
BD47737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81579	WGS84	BD47737	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47737_Msal_Bot2012_COI	ESAV-080	1	BD47737_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47737	Sample	BD47737	targeted	Absent	Sample	Msal_Bot2012_COI	ESAV-080	COI	45	BD47737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-080	GYTAGTTCCRATGATGTTAGCTG	138.46367
BD47737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-34.81579	WGS84	BD47737	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47737_Rhar_Sim2018_COI	ESAV-080	1	BD47737_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47737	Sample	BD47737	targeted	Absent	Sample	Rhar_Sim2018_COI	ESAV-080	COI	45	BD47737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-080	CTGCTGCTATTGCTCATG	138.46367
BD47739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.8171	WGS84	BD47739	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47739_Pcan_Bot2011_IGS	MA100	1	BD47739_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47739	Sample	BD47739	targeted	Absent	Sample	Pcan_Bot2011_IGS	MA100	IGS	45	BD47739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA100	CGTAATCCTCAGTACTGGCTA	151.23818
BD47739	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.8171	WGS84	BD47739	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/02/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47739_Vgib_Wil2025_COI	MA100	1	BD47739_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47739	Sample	BD47739	targeted	Absent	Sample	Vgib_Wil2025_COI	MA100	COI	45	BD47739	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA100	CCCCTCTTTACAAGTTCTTTAGGGT	151.23818
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BD47740_Hsan_Wil2021_COI	GB65	1	BD47740_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Hsan_Wil2021_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	CCTGGGCCGGTATAGTAGGT	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47740_Mros_Gib2022_COI	GB65	1	BD47740_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Mros_Gib2022_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BD47740_Msal_Bot2012_COI	GB65	1	BD47740_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Msal_Bot2012_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	GYTAGTTCCRATGATGTTAGCTG	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47740_Pvir_Dia2013_COI	GB65	1	BD47740_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Pvir_Dia2013_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	CTTAGTGGCATTAATTCGDAATCC	151.2453
BD47740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.82563	WGS84	BD47740	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BD47740_Rhar_Sim2018_COI	GB65	1	BD47740_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47740	Sample	BD47740	targeted	Absent	Sample	Rhar_Sim2018_COI	GB65	COI	45	BD47740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB65	CTGCTGCTATTGCTCATG	151.2453
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47742_Pcan_Bot2011_IGS	GB62	1	BD47742_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB62	IGS	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	CGTAATCCTCAGTACTGGCTA	151.178
BD47742	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.79134	WGS84	BD47742	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47742_Vgib_Wil2025_COI	GB62	1	BD47742_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47742	Sample	BD47742	targeted	Absent	Sample	Vgib_Wil2025_COI	GB62	COI	45	BD47742	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB62	CCCCTCTTTACAAGTTCTTTAGGGT	151.178
BD47743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14739	-23.85192	WGS84	BD47743	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Winter	31/07/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47743_Asen_Wil2025_COI	GA34	1	BD47743_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47743	Sample	BD47743	targeted	Absent	Sample	Asen_Wil2025_COI	GA34	COI	45	BD47743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GA34	GTTGTTGTTACAACACATGCTCTTTTG	151.30959
BD47744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80229	WGS84	BD47744	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47744_Mros_Gib2022_COI	ESAV-187	1	BD47744_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47744	Sample	BD47744	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-187	COI	45	BD47744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-187	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47662
BD47744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80229	WGS84	BD47744	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47744_Pvir_Dia2013_COI	ESAV-187	1	BD47744_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47744	Sample	BD47744	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-187	COI	45	BD47744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-187	CTTAGTGGCATTAATTCGDAATCC	138.47662
BD47748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80569	WGS84	BD47748	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47748_Mros_Gib2022_COI	ESAV-089	1	BD47748_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47748	Sample	BD47748	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-089	COI	45	BD47748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-089	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.46485
BD47748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80569	WGS84	BD47748	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47748_Pvir_Dia2013_COI	ESAV-089	1	BD47748_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47748	Sample	BD47748	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-089	COI	45	BD47748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-089	CTTAGTGGCATTAATTCGDAATCC	138.46485
BD47750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8214	WGS84	BD47750	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47750_Cjap_Sim2018_COI	GB75	1	BD47750_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47750	Sample	BD47750	targeted	Absent	Sample	Cjap_Sim2018_COI	GB75	COI	45	BD47750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB75	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.2451
BD47751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82647	WGS84	BD47751	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47751_Mros_Gib2022_COI	GB30	1	BD47751_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47751	Sample	BD47751	targeted	Absent	Sample	Mros_Gib2022_COI	GB30	COI	45	BD47751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB30	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.24714
BD47751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82647	WGS84	BD47751	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47751_Pvir_Dia2013_COI	GB30	1	BD47751_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47751	Sample	BD47751	targeted	Absent	Sample	Pvir_Dia2013_COI	GB30	COI	45	BD47751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB30	CTTAGTGGCATTAATTCGDAATCC	151.24714
BD47755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.85198	WGS84	BD47755	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47755_Mros_Gib2022_COI	ESAV-133	1	BD47755_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47755	Sample	BD47755	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-133	COI	45	BD47755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-133	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47143
BD47755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.85198	WGS84	BD47755	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47755_Pvir_Dia2013_COI	ESAV-133	1	BD47755_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47755	Sample	BD47755	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-133	COI	45	BD47755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-133	CTTAGTGGCATTAATTCGDAATCC	138.47143
BD47759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.7939	WGS84	BD47759	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47759_Mros_Gib2022_COI	MA110	1	BD47759_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47759	Sample	BD47759	targeted	Absent	Sample	Mros_Gib2022_COI	MA110	COI	45	BD47759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA110	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21309
BD47759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.7939	WGS84	BD47759	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Winter	8/03/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47759_Pvir_Dia2013_COI	MA110	1	BD47759_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47759	Sample	BD47759	targeted	Absent	Sample	Pvir_Dia2013_COI	MA110	COI	45	BD47759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	MA110	CTTAGTGGCATTAATTCGDAATCC	151.21309
BD47762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82353	WGS84	BD47762	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47762_Mros_Gib2022_COI	GC48	1	BD47762_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47762	Sample	BD47762	targeted	Absent	Sample	Mros_Gib2022_COI	GC48	COI	45	BD47762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC48	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.24316
BD47762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82353	WGS84	BD47762	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	28/02/2018	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47762_Pvir_Dia2013_COI	GC48	1	BD47762_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47762	Sample	BD47762	targeted	Absent	Sample	Pvir_Dia2013_COI	GC48	COI	45	BD47762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC48	CTTAGTGGCATTAATTCGDAATCC	151.24316
BD47766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84552	WGS84	BD47766	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47766_Mros_Gib2022_COI	ESAV-138	1	BD47766_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47766	Sample	BD47766	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-138	COI	45	BD47766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-138	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.47092
BD47766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84552	WGS84	BD47766	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47766_Pvir_Dia2013_COI	ESAV-138	1	BD47766_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47766	Sample	BD47766	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-138	COI	45	BD47766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-138	CTTAGTGGCATTAATTCGDAATCC	138.47092
BD47773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82461	WGS84	BD47773	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47773_Mros_Gib2022_COI	GB32	1	BD47773_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47773	Sample	BD47773	targeted	Absent	Sample	Mros_Gib2022_COI	GB32	COI	45	BD47773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB32	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.24755
BD47773	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82461	WGS84	BD47773	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	31/10/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47773_Pvir_Dia2013_COI	GB32	1	BD47773_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47773	Sample	BD47773	targeted	Absent	Sample	Pvir_Dia2013_COI	GB32	COI	45	BD47773	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB32	CTTAGTGGCATTAATTCGDAATCC	151.24755
BD47777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86687	WGS84	BD47777	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47777_Mros_Gib2022_COI	ESAV-119	1	BD47777_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47777	Sample	BD47777	targeted	Absent	Sample	Mros_Gib2022_COI	ESAV-119	COI	45	BD47777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-119	ACTTCTACCTCCTGCTCTTCTACTTCTC	138.4668
BD47777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86687	WGS84	BD47777	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47777_Pvir_Dia2013_COI	ESAV-119	1	BD47777_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47777	Sample	BD47777	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-119	COI	45	BD47777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-119	CTTAGTGGCATTAATTCGDAATCC	138.4668
BD47780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79329	WGS84	BD47780	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47780_Mros_Gib2022_COI	GB59	1	BD47780_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47780	Sample	BD47780	targeted	Absent	Sample	Mros_Gib2022_COI	GB59	COI	45	BD47780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB59	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18479
BD47780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.79329	WGS84	BD47780	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47780_Pvir_Dia2013_COI	GB59	1	BD47780_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47780	Sample	BD47780	targeted	Absent	Sample	Pvir_Dia2013_COI	GB59	COI	45	BD47780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB59	CTTAGTGGCATTAATTCGDAATCC	151.18479
BD47784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80632	WGS84	BD47784	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47784_Mros_Gib2022_COI	GB39	1	BD47784_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47784	Sample	BD47784	targeted	Absent	Sample	Mros_Gib2022_COI	GB39	COI	45	BD47784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB39	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.21532
BD47784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80632	WGS84	BD47784	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47784_Pvir_Dia2013_COI	GB39	1	BD47784_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47784	Sample	BD47784	targeted	Absent	Sample	Pvir_Dia2013_COI	GB39	COI	45	BD47784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB39	CTTAGTGGCATTAATTCGDAATCC	151.21532
BD47787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.85994	WGS84	BD47787	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47787_Cjap_Sim2018_COI	ESAV-126	1	BD47787_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47787	Sample	BD47787	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-126	COI	45	BD47787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-126	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46972
BD47790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.84037	WGS84	BD47790	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	23/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47790_Cjap_Sim2018_COI	ESAV-051	1	BD47790_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47790	Sample	BD47790	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-051	COI	45	BD47790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-051	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46353
BD47794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82305	WGS84	BD47794	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BD47794_Asen_Wil2025_COI	GB72	1	BD47794_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47794	Sample	BD47794	targeted	Absent	Sample	Asen_Wil2025_COI	GB72	COI	45	BD47794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB72	GTTGTTGTTACAACACATGCTCTTTTG	151.24253
BD47794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82305	WGS84	BD47794	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47794_Cjap_Sim2018_COI	GB72	1	BD47794_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47794	Sample	BD47794	targeted	Absent	Sample	Cjap_Sim2018_COI	GB72	COI	45	BD47794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB72	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.24253
BD47794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.82305	WGS84	BD47794	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BD47794_Mjap_Gib2022_COI	GB72	1	BD47794_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47794	Sample	BD47794	targeted	Absent	Sample	Mjap_Gib2022_COI	GB72	COI	45	BD47794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB72	AATTTAATTGTGACTAGGCATGGTCTG	151.24253
BD47798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.83395	WGS84	BD47798	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47798_Cjap_Sim2018_COI	ESAV-145	1	BD47798_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47798	Sample	BD47798	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-145	COI	45	BD47798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-145	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47286
BD47802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83531	WGS84	BD47802	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47802_Cjap_Sim2018_COI	GC20	1	BD47802_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47802	Sample	BD47802	targeted	Absent	Sample	Cjap_Sim2018_COI	GC20	COI	45	BD47802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC20	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26975
BD47806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.71776	WGS84	BD47806	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Port_Lincoln_Summer	29/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47806_Cjap_Sim2018_COI	ESAV-190	1	BD47806_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47806	Sample	BD47806	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-190	COI	45	BD47806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-190	TTAATATACGGTCATTTGGTATGAGTATAGATC	135.88088
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BD47809_Pcan_Bot2011_IGS	GB86	1	BD47809_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Pcan_Bot2011_IGS	GB86	IGS	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	CGTAATCCTCAGTACTGGCTA	151.19615
BD47809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.77904	WGS84	BD47809	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47809_Vgib_Wil2025_COI	GB86	1	BD47809_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47809	Sample	BD47809	targeted	Absent	Sample	Vgib_Wil2025_COI	GB86	COI	45	BD47809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB86	CCCCTCTTTACAAGTTCTTTAGGGT	151.19615
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47810_Mros_Gib2022_COI	GB41	1	BD47810_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Mros_Gib2022_COI	GB41	COI	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2175
BD47810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.80687	WGS84	BD47810	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/01/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47810_Pvir_Dia2013_COI	GB41	1	BD47810_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47810	Sample	BD47810	targeted	Absent	Sample	Pvir_Dia2013_COI	GB41	COI	45	BD47810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB41	CTTAGTGGCATTAATTCGDAATCC	151.2175
BD47812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.80529	WGS84	BD47812	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47812_Vgib_Wil2025_COI	ESAV-173	1	BD47812_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47812	Sample	BD47812	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-173	COI	45	BD47812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-173	CCCCTCTTTACAAGTTCTTTAGGGT	138.48388
BD47813	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.86431	WGS84	BD47813	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47813_Cjap_Sim2018_COI	ESAV-109	1	BD47813_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47813	Sample	BD47813	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-109	COI	45	BD47813	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-109	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.46528
BD47814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8156	WGS84	BD47814	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BD47814_Mros_Gib2022_COI	GB81	1	BD47814_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47814	Sample	BD47814	targeted	Absent	Sample	Mros_Gib2022_COI	GB81	COI	45	BD47814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB81	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.23658
BD47814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.8156	WGS84	BD47814	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	Molecular_validation_surveys-Gladstone_Spring	11/02/2017	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47814_Pvir_Dia2013_COI	GB81	1	BD47814_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47814	Sample	BD47814	targeted	Absent	Sample	Pvir_Dia2013_COI	GB81	COI	45	BD47814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GB81	CTTAGTGGCATTAATTCGDAATCC	151.23658
BD47816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.85356	WGS84	BD47816	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47816_Vgib_Wil2025_COI	ESAV-098	1	BD47816_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47816	Sample	BD47816	targeted	Absent	Sample	Vgib_Wil2025_COI	ESAV-098	COI	45	BD47816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-098	CCCCTCTTTACAAGTTCTTTAGGGT	138.46576
BD47817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.95886	WGS84	BD47817	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021-22_Molecular_surveys-Klein_Point_Winter	29/09/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47817_Cjap_Sim2018_COI	KPT-A39	1	BD47817_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47817	Sample	BD47817	targeted	Absent	Sample	Cjap_Sim2018_COI	KPT-A39	COI	45	BD47817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	KPT-A39	TTAATATACGGTCATTTGGTATGAGTATAGATC	137.77591
BD47818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.79976	WGS84	BD47818	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	29/11/2021	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BD47818_Pvir_Dia2013_COI	ESAV-184	1	BD47818_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47818	Sample	BD47818	targeted	Absent	Sample	Pvir_Dia2013_COI	ESAV-184	COI	45	BD47818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-184	CTTAGTGGCATTAATTCGDAATCC	138.47774
BD47820	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-34.85365	WGS84	BD47820	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Willan RC, Ellard K, Deveney MR (2023) Assessment of analytical and field performance of qPCR assays for priority marine pests. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences),  SARDI Publication No. F2023/000164-1. SARDI Research Report Series No. 1189, Adelaide.	2021_Assay_validation_sampling-Exotic_species_validation	24/11/2021	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BD47820_Cjap_Sim2018_COI	ESAV-132	1	BD47820_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47820	Sample	BD47820	targeted	Absent	Sample	Cjap_Sim2018_COI	ESAV-132	COI	45	BD47820	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	ESAV-132	TTAATATACGGTCATTTGGTATGAGTATAGATC	138.47161
BD47823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_14711	-23.83627	WGS84	BD47823	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Development and validation of  quantitative PCR assays for the invasive bivalves, Arcuatula senhousia and Varicorbula gibba. Report to the Department of Agriculture, Fisheries and Forestry. South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	Molecular_validation_surveys-Gladstone_Summer	27/02/2018	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BD47823_Vgib_Wil2025_COI	GC17	1	BD47823_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 50 micron Plankton net	BD47823	Sample	BD47823	targeted	Absent	Sample	Vgib_Wil2025_COI	GC17	COI	45	BD47823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GC17	CCCCTCTTTACAAGTTCTTTAGGGT	151.2724
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11308_Aamu_Bax2006_COI	CNSA-002	1	BE11308_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	GCACAACCGGGATCTTTACTTC	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11308_Pcan_Bot2011_IGS	CNSA-002	1	BE11308_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-002	IGS	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	CGTAATCCTCAGTACTGGCTA	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11308_Pper_Dia2013_COI	CNSA-002	1	BE11308_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	CTTAGTGGCATTAATTCGDAATCC	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11308_Sspa_Oph2007_COI	CNSA-002	1	BE11308_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	GAAGGCCTTCCGATGATC	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11308_Upin_Bot2011_COI	CNSA-002	1	BE11308_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	CTTTAATTACAGCGTTTTTATTGTTGT	145.77346
BE11308	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94733	WGS84	BE11308	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11308_Vgib_Wil2025_COI	CNSA-002	1	BE11308_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11308	Sample	BE11308	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-002	COI	45	BE11308	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-002	CCCCTCTTTACAAGTTCTTTAGGGT	145.77346
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11309_Asen_Wil2025_COI	CNSA-003	1	BE11309_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	GTTGTTGTTACAACACATGCTCTTTTG	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11309_Cjap_Sim2018_COI	CNSA-003	1	BE11309_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11309_Esin_And2018_Cyb	CNSA-003	1	BE11309_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-003	Cytb	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	ACCCCTCCTCATATCCAACCA	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11309_Mgig_Bot2012_COI	CNSA-003	1	BE11309_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	TCTTATTCGTTGGAGACTTTATAACCCT	145.77464
BE11309	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94508	WGS84	BE11309	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11309_Mjap_Gib2022_COI	CNSA-003	1	BE11309_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11309	Sample	BE11309	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-003	COI	45	BE11309	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-003	AATTTAATTGTGACTAGGCATGGTCTG	145.77464
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11311_Aamu_Bax2006_COI	CNSA-005	1	BE11311_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	GCACAACCGGGATCTTTACTTC	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11311_Pcan_Bot2011_IGS	CNSA-005	1	BE11311_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-005	IGS	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	CGTAATCCTCAGTACTGGCTA	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11311_Pper_Dia2013_COI	CNSA-005	1	BE11311_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	CTTAGTGGCATTAATTCGDAATCC	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11311_Sspa_Oph2007_COI	CNSA-005	1	BE11311_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	GAAGGCCTTCCGATGATC	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11311_Upin_Bot2011_COI	CNSA-005	1	BE11311_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	CTTTAATTACAGCGTTTTTATTGTTGT	145.77936
BE11311	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94188	WGS84	BE11311	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11311_Vgib_Wil2025_COI	CNSA-005	1	BE11311_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11311	Sample	BE11311	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-005	COI	45	BE11311	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-005	CCCCTCTTTACAAGTTCTTTAGGGT	145.77936
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11312_Asen_Wil2025_COI	CNSA-006	1	BE11312_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	GTTGTTGTTACAACACATGCTCTTTTG	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11312_Cjap_Sim2018_COI	CNSA-006	1	BE11312_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11312_Esin_And2018_Cyb	CNSA-006	1	BE11312_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-006	Cytb	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	ACCCCTCCTCATATCCAACCA	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11312_Mgig_Bot2012_COI	CNSA-006	1	BE11312_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	TCTTATTCGTTGGAGACTTTATAACCCT	145.78256
BE11312	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.9426	WGS84	BE11312	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11312_Mjap_Gib2022_COI	CNSA-006	1	BE11312_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11312	Sample	BE11312	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-006	COI	45	BE11312	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-006	AATTTAATTGTGACTAGGCATGGTCTG	145.78256
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11313_Cmae_Bot2011_COI	CNSA-007	1	BE11313_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	ATGAACAGTCTATCCTCCTTTAG	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11313_Hsan_Wil2021_COI	CNSA-007	1	BE11313_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	CCTGGGCCGGTATAGTAGGT	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11313_Mros_Gib2022_COI	CNSA-007	1	BE11313_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11313_Msal_Bot2012_COI	CNSA-007	1	BE11313_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	GYTAGTTCCRATGATGTTAGCTG	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11313_Mstr_Wil2021_COI	CNSA-007	1	BE11313_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	GTAGTGGTAACTACTCATGCTTTA	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11313_Pvir_Dia2013_COI	CNSA-007	1	BE11313_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	CTTAGTGGCATTAATTCGDAATCC	145.7816
BE11313	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.94121	WGS84	BE11313	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11313_Rhar_Sim2018_COI	CNSA-007	1	BE11313_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11313	Sample	BE11313	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-007	COI	45	BE11313	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-007	CTGCTGCTATTGCTCATG	145.7816
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11315_Aamu_Bax2006_COI	CNSA-009	1	BE11315_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	GCACAACCGGGATCTTTACTTC	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11315_Pcan_Bot2011_IGS	CNSA-009	1	BE11315_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-009	IGS	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	CGTAATCCTCAGTACTGGCTA	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11315_Pper_Dia2013_COI	CNSA-009	1	BE11315_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	CTTAGTGGCATTAATTCGDAATCC	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11315_Sspa_Oph2007_COI	CNSA-009	1	BE11315_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	GAAGGCCTTCCGATGATC	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11315_Upin_Bot2011_COI	CNSA-009	1	BE11315_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	CTTTAATTACAGCGTTTTTATTGTTGT	145.78196
BE11315	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93893	WGS84	BE11315	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11315_Vgib_Wil2025_COI	CNSA-009	1	BE11315_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11315	Sample	BE11315	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-009	COI	45	BE11315	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-009	CCCCTCTTTACAAGTTCTTTAGGGT	145.78196
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11316_Asen_Wil2025_COI	CNSA-010	1	BE11316_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	GTTGTTGTTACAACACATGCTCTTTTG	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11316_Cjap_Sim2018_COI	CNSA-010	1	BE11316_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11316_Esin_And2018_Cyb	CNSA-010	1	BE11316_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-010	Cytb	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	ACCCCTCCTCATATCCAACCA	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11316_Mgig_Bot2012_COI	CNSA-010	1	BE11316_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	TCTTATTCGTTGGAGACTTTATAACCCT	145.78192
BE11316	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93761	WGS84	BE11316	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11316_Mjap_Gib2022_COI	CNSA-010	1	BE11316_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11316	Sample	BE11316	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-010	COI	45	BE11316	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-010	AATTTAATTGTGACTAGGCATGGTCTG	145.78192
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11317_Cmae_Bot2011_COI	CNSA-011	1	BE11317_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	ATGAACAGTCTATCCTCCTTTAG	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11317_Hsan_Wil2021_COI	CNSA-011	1	BE11317_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	CCTGGGCCGGTATAGTAGGT	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11317_Mros_Gib2022_COI	CNSA-011	1	BE11317_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11317_Msal_Bot2012_COI	CNSA-011	1	BE11317_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	GYTAGTTCCRATGATGTTAGCTG	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11317_Mstr_Wil2021_COI	CNSA-011	1	BE11317_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	GTAGTGGTAACTACTCATGCTTTA	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11317_Pvir_Dia2013_COI	CNSA-011	1	BE11317_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	CTTAGTGGCATTAATTCGDAATCC	145.78089
BE11317	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93759	WGS84	BE11317	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11317_Rhar_Sim2018_COI	CNSA-011	1	BE11317_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11317	Sample	BE11317	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-011	COI	45	BE11317	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-011	CTGCTGCTATTGCTCATG	145.78089
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11319_Aamu_Bax2006_COI	CNSA-013	1	BE11319_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	GCACAACCGGGATCTTTACTTC	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11319_Pcan_Bot2011_IGS	CNSA-013	1	BE11319_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-013	IGS	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	CGTAATCCTCAGTACTGGCTA	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11319_Pper_Dia2013_COI	CNSA-013	1	BE11319_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	CTTAGTGGCATTAATTCGDAATCC	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11319_Sspa_Oph2007_COI	CNSA-013	1	BE11319_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	GAAGGCCTTCCGATGATC	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11319_Upin_Bot2011_COI	CNSA-013	1	BE11319_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	CTTTAATTACAGCGTTTTTATTGTTGT	145.78071
BE11319	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93474	WGS84	BE11319	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11319_Vgib_Wil2025_COI	CNSA-013	1	BE11319_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11319	Sample	BE11319	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-013	COI	45	BE11319	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-013	CCCCTCTTTACAAGTTCTTTAGGGT	145.78071
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11320_Cmae_Bot2011_COI	CNSA-014	1	BE11320_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	ATGAACAGTCTATCCTCCTTTAG	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11320_Hsan_Wil2021_COI	CNSA-014	1	BE11320_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	CCTGGGCCGGTATAGTAGGT	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11320_Mros_Gib2022_COI	CNSA-014	1	BE11320_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11320_Msal_Bot2012_COI	CNSA-014	1	BE11320_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	GYTAGTTCCRATGATGTTAGCTG	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11320_Mstr_Wil2021_COI	CNSA-014	1	BE11320_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	GTAGTGGTAACTACTCATGCTTTA	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11320_Pvir_Dia2013_COI	CNSA-014	1	BE11320_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	CTTAGTGGCATTAATTCGDAATCC	145.78207
BE11320	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93471	WGS84	BE11320	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11320_Rhar_Sim2018_COI	CNSA-014	1	BE11320_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11320	Sample	BE11320	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-014	COI	45	BE11320	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-014	CTGCTGCTATTGCTCATG	145.78207
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11322_Aamu_Bax2006_COI	CNSA-016	1	BE11322_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	GCACAACCGGGATCTTTACTTC	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11322_Pcan_Bot2011_IGS	CNSA-016	1	BE11322_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-016	IGS	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	CGTAATCCTCAGTACTGGCTA	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11322_Pper_Dia2013_COI	CNSA-016	1	BE11322_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	CTTAGTGGCATTAATTCGDAATCC	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11322_Sspa_Oph2007_COI	CNSA-016	1	BE11322_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	GAAGGCCTTCCGATGATC	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11322_Upin_Bot2011_COI	CNSA-016	1	BE11322_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	CTTTAATTACAGCGTTTTTATTGTTGT	145.78048
BE11322	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93189	WGS84	BE11322	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11322_Vgib_Wil2025_COI	CNSA-016	1	BE11322_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11322	Sample	BE11322	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-016	COI	45	BE11322	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-016	CCCCTCTTTACAAGTTCTTTAGGGT	145.78048
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11323_Asen_Wil2025_COI	CNSA-017	1	BE11323_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	GTTGTTGTTACAACACATGCTCTTTTG	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11323_Cjap_Sim2018_COI	CNSA-017	1	BE11323_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11323_Esin_And2018_Cyb	CNSA-017	1	BE11323_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-017	Cytb	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	ACCCCTCCTCATATCCAACCA	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11323_Mgig_Bot2012_COI	CNSA-017	1	BE11323_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	TCTTATTCGTTGGAGACTTTATAACCCT	145.78215
BE11323	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93174	WGS84	BE11323	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11323_Mjap_Gib2022_COI	CNSA-017	1	BE11323_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11323	Sample	BE11323	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-017	COI	45	BE11323	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-017	AATTTAATTGTGACTAGGCATGGTCTG	145.78215
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11324_Cmae_Bot2011_COI	CNSA-018	1	BE11324_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	ATGAACAGTCTATCCTCCTTTAG	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11324_Hsan_Wil2021_COI	CNSA-018	1	BE11324_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	CCTGGGCCGGTATAGTAGGT	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11324_Mros_Gib2022_COI	CNSA-018	1	BE11324_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11324_Msal_Bot2012_COI	CNSA-018	1	BE11324_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	GYTAGTTCCRATGATGTTAGCTG	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11324_Mstr_Wil2021_COI	CNSA-018	1	BE11324_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	GTAGTGGTAACTACTCATGCTTTA	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11324_Pvir_Dia2013_COI	CNSA-018	1	BE11324_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	CTTAGTGGCATTAATTCGDAATCC	145.78129
BE11324	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.93041	WGS84	BE11324	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11324_Rhar_Sim2018_COI	CNSA-018	1	BE11324_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11324	Sample	BE11324	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-018	COI	45	BE11324	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-018	CTGCTGCTATTGCTCATG	145.78129
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11326_Aamu_Bax2006_COI	CNSA-020	1	BE11326_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	GCACAACCGGGATCTTTACTTC	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11326_Pcan_Bot2011_IGS	CNSA-020	1	BE11326_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-020	IGS	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	CGTAATCCTCAGTACTGGCTA	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11326_Pper_Dia2013_COI	CNSA-020	1	BE11326_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	CTTAGTGGCATTAATTCGDAATCC	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11326_Sspa_Oph2007_COI	CNSA-020	1	BE11326_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	GAAGGCCTTCCGATGATC	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11326_Upin_Bot2011_COI	CNSA-020	1	BE11326_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	CTTTAATTACAGCGTTTTTATTGTTGT	145.78211
BE11326	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92899	WGS84	BE11326	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11326_Vgib_Wil2025_COI	CNSA-020	1	BE11326_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11326	Sample	BE11326	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-020	COI	45	BE11326	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-020	CCCCTCTTTACAAGTTCTTTAGGGT	145.78211
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11327_Asen_Wil2025_COI	CNSA-021	1	BE11327_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	GTTGTTGTTACAACACATGCTCTTTTG	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11327_Cjap_Sim2018_COI	CNSA-021	1	BE11327_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11327_Esin_And2018_Cyb	CNSA-021	1	BE11327_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-021	Cytb	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	ACCCCTCCTCATATCCAACCA	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11327_Mgig_Bot2012_COI	CNSA-021	1	BE11327_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	TCTTATTCGTTGGAGACTTTATAACCCT	145.7835
BE11327	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92876	WGS84	BE11327	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11327_Mjap_Gib2022_COI	CNSA-021	1	BE11327_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11327	Sample	BE11327	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-021	COI	45	BE11327	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-021	AATTTAATTGTGACTAGGCATGGTCTG	145.7835
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11328_Cmae_Bot2011_COI	CNSA-022	1	BE11328_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	ATGAACAGTCTATCCTCCTTTAG	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11328_Hsan_Wil2021_COI	CNSA-022	1	BE11328_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	CCTGGGCCGGTATAGTAGGT	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11328_Mros_Gib2022_COI	CNSA-022	1	BE11328_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11328_Msal_Bot2012_COI	CNSA-022	1	BE11328_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	GYTAGTTCCRATGATGTTAGCTG	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11328_Mstr_Wil2021_COI	CNSA-022	1	BE11328_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	GTAGTGGTAACTACTCATGCTTTA	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11328_Pvir_Dia2013_COI	CNSA-022	1	BE11328_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	CTTAGTGGCATTAATTCGDAATCC	145.78172
BE11328	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92606	WGS84	BE11328	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11328_Rhar_Sim2018_COI	CNSA-022	1	BE11328_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11328	Sample	BE11328	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-022	COI	45	BE11328	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-022	CTGCTGCTATTGCTCATG	145.78172
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11330_Asen_Wil2025_COI	CNSA-024	1	BE11330_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	GTTGTTGTTACAACACATGCTCTTTTG	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11330_Cjap_Sim2018_COI	CNSA-024	1	BE11330_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11330_Esin_And2018_Cyb	CNSA-024	1	BE11330_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-024	Cytb	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	ACCCCTCCTCATATCCAACCA	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11330_Mgig_Bot2012_COI	CNSA-024	1	BE11330_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	TCTTATTCGTTGGAGACTTTATAACCCT	145.78272
BE11330	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92482	WGS84	BE11330	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11330_Mjap_Gib2022_COI	CNSA-024	1	BE11330_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11330	Sample	BE11330	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-024	COI	45	BE11330	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-024	AATTTAATTGTGACTAGGCATGGTCTG	145.78272
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11331_Cmae_Bot2011_COI	CNSA-025	1	BE11331_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	ATGAACAGTCTATCCTCCTTTAG	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11331_Hsan_Wil2021_COI	CNSA-025	1	BE11331_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	CCTGGGCCGGTATAGTAGGT	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11331_Mros_Gib2022_COI	CNSA-025	1	BE11331_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11331_Msal_Bot2012_COI	CNSA-025	1	BE11331_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	GYTAGTTCCRATGATGTTAGCTG	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11331_Mstr_Wil2021_COI	CNSA-025	1	BE11331_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	GTAGTGGTAACTACTCATGCTTTA	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11331_Pvir_Dia2013_COI	CNSA-025	1	BE11331_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	CTTAGTGGCATTAATTCGDAATCC	145.78397
BE11331	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92443	WGS84	BE11331	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11331_Rhar_Sim2018_COI	CNSA-025	1	BE11331_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11331	Sample	BE11331	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-025	COI	45	BE11331	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-025	CTGCTGCTATTGCTCATG	145.78397
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11333_Aamu_Bax2006_COI	CNSA-027	1	BE11333_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	GCACAACCGGGATCTTTACTTC	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11333_Pcan_Bot2011_IGS	CNSA-027	1	BE11333_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-027	IGS	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	CGTAATCCTCAGTACTGGCTA	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11333_Pper_Dia2013_COI	CNSA-027	1	BE11333_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	CTTAGTGGCATTAATTCGDAATCC	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11333_Sspa_Oph2007_COI	CNSA-027	1	BE11333_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	GAAGGCCTTCCGATGATC	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11333_Upin_Bot2011_COI	CNSA-027	1	BE11333_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	CTTTAATTACAGCGTTTTTATTGTTGT	145.78387
BE11333	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92159	WGS84	BE11333	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11333_Vgib_Wil2025_COI	CNSA-027	1	BE11333_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11333	Sample	BE11333	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-027	COI	45	BE11333	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-027	CCCCTCTTTACAAGTTCTTTAGGGT	145.78387
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11334_Asen_Wil2025_COI	CNSA-028	1	BE11334_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	GTTGTTGTTACAACACATGCTCTTTTG	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11334_Cjap_Sim2018_COI	CNSA-028	1	BE11334_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11334_Esin_And2018_Cyb	CNSA-028	1	BE11334_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-028	Cytb	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	ACCCCTCCTCATATCCAACCA	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11334_Mgig_Bot2012_COI	CNSA-028	1	BE11334_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	TCTTATTCGTTGGAGACTTTATAACCCT	145.78504
BE11334	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92157	WGS84	BE11334	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11334_Mjap_Gib2022_COI	CNSA-028	1	BE11334_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11334	Sample	BE11334	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-028	COI	45	BE11334	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-028	AATTTAATTGTGACTAGGCATGGTCTG	145.78504
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11335_Cmae_Bot2011_COI	CNSA-029	1	BE11335_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	ATGAACAGTCTATCCTCCTTTAG	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11335_Hsan_Wil2021_COI	CNSA-029	1	BE11335_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	CCTGGGCCGGTATAGTAGGT	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11335_Mros_Gib2022_COI	CNSA-029	1	BE11335_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11335_Msal_Bot2012_COI	CNSA-029	1	BE11335_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	GYTAGTTCCRATGATGTTAGCTG	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11335_Mstr_Wil2021_COI	CNSA-029	1	BE11335_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	GTAGTGGTAACTACTCATGCTTTA	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11335_Pvir_Dia2013_COI	CNSA-029	1	BE11335_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	CTTAGTGGCATTAATTCGDAATCC	145.78451
BE11335	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92014	WGS84	BE11335	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11335_Rhar_Sim2018_COI	CNSA-029	1	BE11335_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11335	Sample	BE11335	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-029	COI	45	BE11335	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-029	CTGCTGCTATTGCTCATG	145.78451
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11337_Aamu_Bax2006_COI	CNSA-031	1	BE11337_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	GCACAACCGGGATCTTTACTTC	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11337_Pcan_Bot2011_IGS	CNSA-031	1	BE11337_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-031	IGS	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	CGTAATCCTCAGTACTGGCTA	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11337_Pper_Dia2013_COI	CNSA-031	1	BE11337_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	CTTAGTGGCATTAATTCGDAATCC	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11337_Sspa_Oph2007_COI	CNSA-031	1	BE11337_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	GAAGGCCTTCCGATGATC	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11337_Upin_Bot2011_COI	CNSA-031	1	BE11337_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	CTTTAATTACAGCGTTTTTATTGTTGT	145.78462
BE11337	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91781	WGS84	BE11337	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11337_Vgib_Wil2025_COI	CNSA-031	1	BE11337_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11337	Sample	BE11337	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-031	COI	45	BE11337	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-031	CCCCTCTTTACAAGTTCTTTAGGGT	145.78462
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11338_Asen_Wil2025_COI	CNSA-032	1	BE11338_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	GTTGTTGTTACAACACATGCTCTTTTG	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11338_Cjap_Sim2018_COI	CNSA-032	1	BE11338_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11338_Esin_And2018_Cyb	CNSA-032	1	BE11338_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-032	Cytb	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	ACCCCTCCTCATATCCAACCA	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11338_Mgig_Bot2012_COI	CNSA-032	1	BE11338_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	TCTTATTCGTTGGAGACTTTATAACCCT	145.78565
BE11338	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.91801	WGS84	BE11338	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11338_Mjap_Gib2022_COI	CNSA-032	1	BE11338_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11338	Sample	BE11338	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-032	COI	45	BE11338	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-032	AATTTAATTGTGACTAGGCATGGTCTG	145.78565
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11339_Cmae_Bot2011_COI	CNSA-033	1	BE11339_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	ATGAACAGTCTATCCTCCTTTAG	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11339_Hsan_Wil2021_COI	CNSA-033	1	BE11339_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	CCTGGGCCGGTATAGTAGGT	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11339_Mros_Gib2022_COI	CNSA-033	1	BE11339_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Mros_Gib2022_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11339_Msal_Bot2012_COI	CNSA-033	1	BE11339_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Msal_Bot2012_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	GYTAGTTCCRATGATGTTAGCTG	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11339_Mstr_Wil2021_COI	CNSA-033	1	BE11339_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	GTAGTGGTAACTACTCATGCTTTA	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11339_Pvir_Dia2013_COI	CNSA-033	1	BE11339_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	CTTAGTGGCATTAATTCGDAATCC	145.782
BE11339	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92402	WGS84	BE11339	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11339_Rhar_Sim2018_COI	CNSA-033	1	BE11339_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11339	Sample	BE11339	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSA-033	COI	45	BE11339	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-033	CTGCTGCTATTGCTCATG	145.782
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11340_Aamu_Bax2006_COI	CNSA-034	1	BE11340_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	GCACAACCGGGATCTTTACTTC	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11340_Pcan_Bot2011_IGS	CNSA-034	1	BE11340_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSA-034	IGS	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	CGTAATCCTCAGTACTGGCTA	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11340_Pper_Dia2013_COI	CNSA-034	1	BE11340_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Pper_Dia2013_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	CTTAGTGGCATTAATTCGDAATCC	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11340_Sspa_Oph2007_COI	CNSA-034	1	BE11340_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	GAAGGCCTTCCGATGATC	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11340_Upin_Bot2011_COI	CNSA-034	1	BE11340_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Upin_Bot2011_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	CTTTAATTACAGCGTTTTTATTGTTGT	145.7838
BE11340	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92835	WGS84	BE11340	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11340_Vgib_Wil2025_COI	CNSA-034	1	BE11340_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11340	Sample	BE11340	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSA-034	COI	45	BE11340	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-034	CCCCTCTTTACAAGTTCTTTAGGGT	145.7838
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11341_Asen_Wil2025_COI	CNSA-035	1	BE11341_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Asen_Wil2025_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	GTTGTTGTTACAACACATGCTCTTTTG	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11341_Cjap_Sim2018_COI	CNSA-035	1	BE11341_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11341_Esin_And2018_Cyb	CNSA-035	1	BE11341_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Esin_And2018_Cyb	CNSA-035	Cytb	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	ACCCCTCCTCATATCCAACCA	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11341_Mgig_Bot2012_COI	CNSA-035	1	BE11341_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	TCTTATTCGTTGGAGACTTTATAACCCT	145.78241
BE11341	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-16.92836	WGS84	BE11341	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_A	22/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11341_Mjap_Gib2022_COI	CNSA-035	1	BE11341_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11341	Sample	BE11341	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSA-035	COI	45	BE11341	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSA-035	AATTTAATTGTGACTAGGCATGGTCTG	145.78241
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11346_Cmae_Bot2011_COI	TSVA-001	1	BE11346_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	ATGAACAGTCTATCCTCCTTTAG	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11346_Hsan_Wil2021_COI	TSVA-001	1	BE11346_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	CCTGGGCCGGTATAGTAGGT	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11346_Mros_Gib2022_COI	TSVA-001	1	BE11346_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11346_Msal_Bot2012_COI	TSVA-001	1	BE11346_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	GYTAGTTCCRATGATGTTAGCTG	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11346_Mstr_Wil2021_COI	TSVA-001	1	BE11346_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	GTAGTGGTAACTACTCATGCTTTA	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11346_Pvir_Dia2013_COI	TSVA-001	1	BE11346_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	CTTAGTGGCATTAATTCGDAATCC	146.8425
BE11346	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2425	WGS84	BE11346	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11346_Rhar_Sim2018_COI	TSVA-001	1	BE11346_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11346	Sample	BE11346	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-001	COI	45	BE11346	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-001	CTGCTGCTATTGCTCATG	146.8425
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11348_Aamu_Bax2006_COI	TSVA-003	1	BE11348_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	GCACAACCGGGATCTTTACTTC	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11348_Pcan_Bot2011_IGS	TSVA-003	1	BE11348_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-003	IGS	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	CGTAATCCTCAGTACTGGCTA	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11348_Pper_Dia2013_COI	TSVA-003	1	BE11348_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	CTTAGTGGCATTAATTCGDAATCC	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11348_Sspa_Oph2007_COI	TSVA-003	1	BE11348_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	GAAGGCCTTCCGATGATC	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11348_Upin_Bot2011_COI	TSVA-003	1	BE11348_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	CTTTAATTACAGCGTTTTTATTGTTGT	146.8419
BE11348	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24144	WGS84	BE11348	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11348_Vgib_Wil2025_COI	TSVA-003	1	BE11348_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11348	Sample	BE11348	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-003	COI	45	BE11348	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-003	CCCCTCTTTACAAGTTCTTTAGGGT	146.8419
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11349_Asen_Wil2025_COI	TSVA-004	1	BE11349_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	GTTGTTGTTACAACACATGCTCTTTTG	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11349_Cjap_Sim2018_COI	TSVA-004	1	BE11349_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11349_Esin_And2018_Cyb	TSVA-004	1	BE11349_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-004	Cytb	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	ACCCCTCCTCATATCCAACCA	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11349_Mgig_Bot2012_COI	TSVA-004	1	BE11349_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	TCTTATTCGTTGGAGACTTTATAACCCT	146.84148
BE11349	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2417	WGS84	BE11349	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11349_Mjap_Gib2022_COI	TSVA-004	1	BE11349_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11349	Sample	BE11349	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-004	COI	45	BE11349	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-004	AATTTAATTGTGACTAGGCATGGTCTG	146.84148
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11351_Aamu_Bax2006_COI	TSVA-006	1	BE11351_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	GCACAACCGGGATCTTTACTTC	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11351_Pcan_Bot2011_IGS	TSVA-006	1	BE11351_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-006	IGS	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	CGTAATCCTCAGTACTGGCTA	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11351_Pper_Dia2013_COI	TSVA-006	1	BE11351_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	CTTAGTGGCATTAATTCGDAATCC	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11351_Sspa_Oph2007_COI	TSVA-006	1	BE11351_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	GAAGGCCTTCCGATGATC	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11351_Upin_Bot2011_COI	TSVA-006	1	BE11351_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	CTTTAATTACAGCGTTTTTATTGTTGT	146.83958
BE11351	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24116	WGS84	BE11351	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11351_Vgib_Wil2025_COI	TSVA-006	1	BE11351_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11351	Sample	BE11351	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-006	COI	45	BE11351	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-006	CCCCTCTTTACAAGTTCTTTAGGGT	146.83958
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11352_Asen_Wil2025_COI	TSVA-007	1	BE11352_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	GTTGTTGTTACAACACATGCTCTTTTG	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11352_Cjap_Sim2018_COI	TSVA-007	1	BE11352_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11352_Esin_And2018_Cyb	TSVA-007	1	BE11352_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-007	Cytb	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	ACCCCTCCTCATATCCAACCA	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11352_Mgig_Bot2012_COI	TSVA-007	1	BE11352_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	TCTTATTCGTTGGAGACTTTATAACCCT	146.84007
BE11352	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23994	WGS84	BE11352	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11352_Mjap_Gib2022_COI	TSVA-007	1	BE11352_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11352	Sample	BE11352	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-007	COI	45	BE11352	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-007	AATTTAATTGTGACTAGGCATGGTCTG	146.84007
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11353_Cmae_Bot2011_COI	TSVA-008	1	BE11353_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	ATGAACAGTCTATCCTCCTTTAG	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11353_Hsan_Wil2021_COI	TSVA-008	1	BE11353_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	CCTGGGCCGGTATAGTAGGT	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11353_Mros_Gib2022_COI	TSVA-008	1	BE11353_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11353_Msal_Bot2012_COI	TSVA-008	1	BE11353_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	GYTAGTTCCRATGATGTTAGCTG	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11353_Mstr_Wil2021_COI	TSVA-008	1	BE11353_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	GTAGTGGTAACTACTCATGCTTTA	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11353_Pvir_Dia2013_COI	TSVA-008	1	BE11353_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	CTTAGTGGCATTAATTCGDAATCC	146.83795
BE11353	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24028	WGS84	BE11353	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11353_Rhar_Sim2018_COI	TSVA-008	1	BE11353_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11353	Sample	BE11353	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-008	COI	45	BE11353	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-008	CTGCTGCTATTGCTCATG	146.83795
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11355_Aamu_Bax2006_COI	TSVA-010	1	BE11355_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	GCACAACCGGGATCTTTACTTC	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11355_Pcan_Bot2011_IGS	TSVA-010	1	BE11355_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-010	IGS	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	CGTAATCCTCAGTACTGGCTA	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11355_Pper_Dia2013_COI	TSVA-010	1	BE11355_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	CTTAGTGGCATTAATTCGDAATCC	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11355_Sspa_Oph2007_COI	TSVA-010	1	BE11355_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	GAAGGCCTTCCGATGATC	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11355_Upin_Bot2011_COI	TSVA-010	1	BE11355_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	CTTTAATTACAGCGTTTTTATTGTTGT	146.84064
BE11355	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23915	WGS84	BE11355	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11355_Vgib_Wil2025_COI	TSVA-010	1	BE11355_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11355	Sample	BE11355	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-010	COI	45	BE11355	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-010	CCCCTCTTTACAAGTTCTTTAGGGT	146.84064
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11356_Asen_Wil2025_COI	TSVA-011	1	BE11356_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	GTTGTTGTTACAACACATGCTCTTTTG	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11356_Cjap_Sim2018_COI	TSVA-011	1	BE11356_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11356_Esin_And2018_Cyb	TSVA-011	1	BE11356_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-011	Cytb	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	ACCCCTCCTCATATCCAACCA	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11356_Mgig_Bot2012_COI	TSVA-011	1	BE11356_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	TCTTATTCGTTGGAGACTTTATAACCCT	146.84188
BE11356	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23956	WGS84	BE11356	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11356_Mjap_Gib2022_COI	TSVA-011	1	BE11356_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11356	Sample	BE11356	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-011	COI	45	BE11356	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-011	AATTTAATTGTGACTAGGCATGGTCTG	146.84188
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11357_Cmae_Bot2011_COI	TSVA-012	1	BE11357_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	ATGAACAGTCTATCCTCCTTTAG	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11357_Hsan_Wil2021_COI	TSVA-012	1	BE11357_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	CCTGGGCCGGTATAGTAGGT	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11357_Mros_Gib2022_COI	TSVA-012	1	BE11357_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11357_Msal_Bot2012_COI	TSVA-012	1	BE11357_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	GYTAGTTCCRATGATGTTAGCTG	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11357_Mstr_Wil2021_COI	TSVA-012	1	BE11357_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	GTAGTGGTAACTACTCATGCTTTA	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11357_Pvir_Dia2013_COI	TSVA-012	1	BE11357_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	CTTAGTGGCATTAATTCGDAATCC	146.83955
BE11357	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23704	WGS84	BE11357	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11357_Rhar_Sim2018_COI	TSVA-012	1	BE11357_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11357	Sample	BE11357	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-012	COI	45	BE11357	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-012	CTGCTGCTATTGCTCATG	146.83955
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11359_Aamu_Bax2006_COI	TSVA-014	1	BE11359_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	GCACAACCGGGATCTTTACTTC	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11359_Pcan_Bot2011_IGS	TSVA-014	1	BE11359_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-014	IGS	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	CGTAATCCTCAGTACTGGCTA	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11359_Pper_Dia2013_COI	TSVA-014	1	BE11359_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	CTTAGTGGCATTAATTCGDAATCC	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11359_Sspa_Oph2007_COI	TSVA-014	1	BE11359_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	GAAGGCCTTCCGATGATC	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11359_Upin_Bot2011_COI	TSVA-014	1	BE11359_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	CTTTAATTACAGCGTTTTTATTGTTGT	146.83647
BE11359	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2373	WGS84	BE11359	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11359_Vgib_Wil2025_COI	TSVA-014	1	BE11359_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11359	Sample	BE11359	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-014	COI	45	BE11359	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-014	CCCCTCTTTACAAGTTCTTTAGGGT	146.83647
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11360_Cmae_Bot2011_COI	TSVA-015	1	BE11360_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	ATGAACAGTCTATCCTCCTTTAG	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11360_Hsan_Wil2021_COI	TSVA-015	1	BE11360_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	CCTGGGCCGGTATAGTAGGT	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11360_Mros_Gib2022_COI	TSVA-015	1	BE11360_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11360_Msal_Bot2012_COI	TSVA-015	1	BE11360_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	GYTAGTTCCRATGATGTTAGCTG	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11360_Mstr_Wil2021_COI	TSVA-015	1	BE11360_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	GTAGTGGTAACTACTCATGCTTTA	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11360_Pvir_Dia2013_COI	TSVA-015	1	BE11360_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	CTTAGTGGCATTAATTCGDAATCC	146.83612
BE11360	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23738	WGS84	BE11360	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11360_Rhar_Sim2018_COI	TSVA-015	1	BE11360_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11360	Sample	BE11360	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-015	COI	45	BE11360	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-015	CTGCTGCTATTGCTCATG	146.83612
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11362_Aamu_Bax2006_COI	TSVA-017	1	BE11362_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	GCACAACCGGGATCTTTACTTC	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11362_Pcan_Bot2011_IGS	TSVA-017	1	BE11362_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-017	IGS	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	CGTAATCCTCAGTACTGGCTA	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11362_Pper_Dia2013_COI	TSVA-017	1	BE11362_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	CTTAGTGGCATTAATTCGDAATCC	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11362_Sspa_Oph2007_COI	TSVA-017	1	BE11362_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	GAAGGCCTTCCGATGATC	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11362_Upin_Bot2011_COI	TSVA-017	1	BE11362_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	CTTTAATTACAGCGTTTTTATTGTTGT	146.83385
BE11362	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23863	WGS84	BE11362	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11362_Vgib_Wil2025_COI	TSVA-017	1	BE11362_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11362	Sample	BE11362	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-017	COI	45	BE11362	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-017	CCCCTCTTTACAAGTTCTTTAGGGT	146.83385
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11363_Asen_Wil2025_COI	TSVA-018	1	BE11363_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	GTTGTTGTTACAACACATGCTCTTTTG	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11363_Cjap_Sim2018_COI	TSVA-018	1	BE11363_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11363_Esin_And2018_Cyb	TSVA-018	1	BE11363_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-018	Cytb	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	ACCCCTCCTCATATCCAACCA	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11363_Mgig_Bot2012_COI	TSVA-018	1	BE11363_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	TCTTATTCGTTGGAGACTTTATAACCCT	146.83475
BE11363	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23971	WGS84	BE11363	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11363_Mjap_Gib2022_COI	TSVA-018	1	BE11363_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11363	Sample	BE11363	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-018	COI	45	BE11363	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-018	AATTTAATTGTGACTAGGCATGGTCTG	146.83475
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11364_Cmae_Bot2011_COI	TSVA-019	1	BE11364_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	ATGAACAGTCTATCCTCCTTTAG	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11364_Hsan_Wil2021_COI	TSVA-019	1	BE11364_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	CCTGGGCCGGTATAGTAGGT	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11364_Mros_Gib2022_COI	TSVA-019	1	BE11364_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11364_Msal_Bot2012_COI	TSVA-019	1	BE11364_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	GYTAGTTCCRATGATGTTAGCTG	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11364_Mstr_Wil2021_COI	TSVA-019	1	BE11364_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	GTAGTGGTAACTACTCATGCTTTA	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11364_Pvir_Dia2013_COI	TSVA-019	1	BE11364_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	CTTAGTGGCATTAATTCGDAATCC	146.83385
BE11364	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23951	WGS84	BE11364	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11364_Rhar_Sim2018_COI	TSVA-019	1	BE11364_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11364	Sample	BE11364	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-019	COI	45	BE11364	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-019	CTGCTGCTATTGCTCATG	146.83385
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11366_Aamu_Bax2006_COI	TSVA-021	1	BE11366_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	GCACAACCGGGATCTTTACTTC	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11366_Pcan_Bot2011_IGS	TSVA-021	1	BE11366_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-021	IGS	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	CGTAATCCTCAGTACTGGCTA	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11366_Pper_Dia2013_COI	TSVA-021	1	BE11366_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	CTTAGTGGCATTAATTCGDAATCC	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11366_Sspa_Oph2007_COI	TSVA-021	1	BE11366_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	GAAGGCCTTCCGATGATC	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11366_Upin_Bot2011_COI	TSVA-021	1	BE11366_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	CTTTAATTACAGCGTTTTTATTGTTGT	146.83773
BE11366	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23853	WGS84	BE11366	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11366_Vgib_Wil2025_COI	TSVA-021	1	BE11366_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11366	Sample	BE11366	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-021	COI	45	BE11366	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-021	CCCCTCTTTACAAGTTCTTTAGGGT	146.83773
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11367_Asen_Wil2025_COI	TSVA-022	1	BE11367_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	GTTGTTGTTACAACACATGCTCTTTTG	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11367_Cjap_Sim2018_COI	TSVA-022	1	BE11367_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11367_Esin_And2018_Cyb	TSVA-022	1	BE11367_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-022	Cytb	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	ACCCCTCCTCATATCCAACCA	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11367_Mgig_Bot2012_COI	TSVA-022	1	BE11367_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	TCTTATTCGTTGGAGACTTTATAACCCT	146.83784
BE11367	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23733	WGS84	BE11367	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11367_Mjap_Gib2022_COI	TSVA-022	1	BE11367_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11367	Sample	BE11367	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-022	COI	45	BE11367	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-022	AATTTAATTGTGACTAGGCATGGTCTG	146.83784
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11368_Cmae_Bot2011_COI	TSVA-023	1	BE11368_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	ATGAACAGTCTATCCTCCTTTAG	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11368_Hsan_Wil2021_COI	TSVA-023	1	BE11368_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	CCTGGGCCGGTATAGTAGGT	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11368_Mros_Gib2022_COI	TSVA-023	1	BE11368_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11368_Msal_Bot2012_COI	TSVA-023	1	BE11368_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	GYTAGTTCCRATGATGTTAGCTG	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11368_Mstr_Wil2021_COI	TSVA-023	1	BE11368_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	GTAGTGGTAACTACTCATGCTTTA	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11368_Pvir_Dia2013_COI	TSVA-023	1	BE11368_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	CTTAGTGGCATTAATTCGDAATCC	146.83503
BE11368	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.23859	WGS84	BE11368	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11368_Rhar_Sim2018_COI	TSVA-023	1	BE11368_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11368	Sample	BE11368	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-023	COI	45	BE11368	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-023	CTGCTGCTATTGCTCATG	146.83503
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11370_Asen_Wil2025_COI	TSVA-025	1	BE11370_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	GTTGTTGTTACAACACATGCTCTTTTG	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11370_Cjap_Sim2018_COI	TSVA-025	1	BE11370_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11370_Esin_And2018_Cyb	TSVA-025	1	BE11370_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-025	Cytb	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	ACCCCTCCTCATATCCAACCA	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11370_Mgig_Bot2012_COI	TSVA-025	1	BE11370_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	TCTTATTCGTTGGAGACTTTATAACCCT	146.83313
BE11370	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.2452	WGS84	BE11370	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11370_Mjap_Gib2022_COI	TSVA-025	1	BE11370_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11370	Sample	BE11370	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-025	COI	45	BE11370	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-025	AATTTAATTGTGACTAGGCATGGTCTG	146.83313
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11371_Cmae_Bot2011_COI	TSVA-026	1	BE11371_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	ATGAACAGTCTATCCTCCTTTAG	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11371_Hsan_Wil2021_COI	TSVA-026	1	BE11371_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	CCTGGGCCGGTATAGTAGGT	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11371_Mros_Gib2022_COI	TSVA-026	1	BE11371_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11371_Msal_Bot2012_COI	TSVA-026	1	BE11371_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	GYTAGTTCCRATGATGTTAGCTG	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11371_Mstr_Wil2021_COI	TSVA-026	1	BE11371_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	GTAGTGGTAACTACTCATGCTTTA	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11371_Pvir_Dia2013_COI	TSVA-026	1	BE11371_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	CTTAGTGGCATTAATTCGDAATCC	146.83212
BE11371	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24652	WGS84	BE11371	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11371_Rhar_Sim2018_COI	TSVA-026	1	BE11371_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11371	Sample	BE11371	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-026	COI	45	BE11371	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-026	CTGCTGCTATTGCTCATG	146.83212
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11373_Aamu_Bax2006_COI	TSVA-028	1	BE11373_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	GCACAACCGGGATCTTTACTTC	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11373_Pcan_Bot2011_IGS	TSVA-028	1	BE11373_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-028	IGS	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	CGTAATCCTCAGTACTGGCTA	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11373_Pper_Dia2013_COI	TSVA-028	1	BE11373_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	CTTAGTGGCATTAATTCGDAATCC	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11373_Sspa_Oph2007_COI	TSVA-028	1	BE11373_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	GAAGGCCTTCCGATGATC	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11373_Upin_Bot2011_COI	TSVA-028	1	BE11373_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	CTTTAATTACAGCGTTTTTATTGTTGT	146.83453
BE11373	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24613	WGS84	BE11373	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11373_Vgib_Wil2025_COI	TSVA-028	1	BE11373_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11373	Sample	BE11373	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-028	COI	45	BE11373	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-028	CCCCTCTTTACAAGTTCTTTAGGGT	146.83453
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11374_Asen_Wil2025_COI	TSVA-029	1	BE11374_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	GTTGTTGTTACAACACATGCTCTTTTG	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11374_Cjap_Sim2018_COI	TSVA-029	1	BE11374_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11374_Esin_And2018_Cyb	TSVA-029	1	BE11374_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-029	Cytb	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	ACCCCTCCTCATATCCAACCA	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11374_Mgig_Bot2012_COI	TSVA-029	1	BE11374_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	TCTTATTCGTTGGAGACTTTATAACCCT	146.83552
BE11374	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24643	WGS84	BE11374	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11374_Mjap_Gib2022_COI	TSVA-029	1	BE11374_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11374	Sample	BE11374	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-029	COI	45	BE11374	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-029	AATTTAATTGTGACTAGGCATGGTCTG	146.83552
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11375_Cmae_Bot2011_COI	TSVA-030	1	BE11375_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	ATGAACAGTCTATCCTCCTTTAG	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11375_Hsan_Wil2021_COI	TSVA-030	1	BE11375_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	CCTGGGCCGGTATAGTAGGT	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11375_Mros_Gib2022_COI	TSVA-030	1	BE11375_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11375_Msal_Bot2012_COI	TSVA-030	1	BE11375_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	GYTAGTTCCRATGATGTTAGCTG	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11375_Mstr_Wil2021_COI	TSVA-030	1	BE11375_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	GTAGTGGTAACTACTCATGCTTTA	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11375_Pvir_Dia2013_COI	TSVA-030	1	BE11375_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	CTTAGTGGCATTAATTCGDAATCC	146.83475
BE11375	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24705	WGS84	BE11375	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11375_Rhar_Sim2018_COI	TSVA-030	1	BE11375_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11375	Sample	BE11375	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-030	COI	45	BE11375	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-030	CTGCTGCTATTGCTCATG	146.83475
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11377_Aamu_Bax2006_COI	TSVA-032	1	BE11377_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	GCACAACCGGGATCTTTACTTC	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11377_Pcan_Bot2011_IGS	TSVA-032	1	BE11377_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-032	IGS	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	CGTAATCCTCAGTACTGGCTA	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11377_Pper_Dia2013_COI	TSVA-032	1	BE11377_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	CTTAGTGGCATTAATTCGDAATCC	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11377_Sspa_Oph2007_COI	TSVA-032	1	BE11377_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	GAAGGCCTTCCGATGATC	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11377_Upin_Bot2011_COI	TSVA-032	1	BE11377_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	CTTTAATTACAGCGTTTTTATTGTTGT	146.83341
BE11377	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24917	WGS84	BE11377	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11377_Vgib_Wil2025_COI	TSVA-032	1	BE11377_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11377	Sample	BE11377	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-032	COI	45	BE11377	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-032	CCCCTCTTTACAAGTTCTTTAGGGT	146.83341
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11378_Asen_Wil2025_COI	TSVA-033	1	BE11378_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Asen_Wil2025_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	GTTGTTGTTACAACACATGCTCTTTTG	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11378_Cjap_Sim2018_COI	TSVA-033	1	BE11378_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11378_Esin_And2018_Cyb	TSVA-033	1	BE11378_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Esin_And2018_Cyb	TSVA-033	Cytb	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	ACCCCTCCTCATATCCAACCA	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11378_Mgig_Bot2012_COI	TSVA-033	1	BE11378_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	TCTTATTCGTTGGAGACTTTATAACCCT	146.83416
BE11378	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.24923	WGS84	BE11378	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11378_Mjap_Gib2022_COI	TSVA-033	1	BE11378_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11378	Sample	BE11378	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVA-033	COI	45	BE11378	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-033	AATTTAATTGTGACTAGGCATGGTCTG	146.83416
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11379_Cmae_Bot2011_COI	TSVA-034	1	BE11379_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	ATGAACAGTCTATCCTCCTTTAG	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11379_Hsan_Wil2021_COI	TSVA-034	1	BE11379_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	CCTGGGCCGGTATAGTAGGT	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11379_Mros_Gib2022_COI	TSVA-034	1	BE11379_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Mros_Gib2022_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11379_Msal_Bot2012_COI	TSVA-034	1	BE11379_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Msal_Bot2012_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	GYTAGTTCCRATGATGTTAGCTG	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11379_Mstr_Wil2021_COI	TSVA-034	1	BE11379_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	GTAGTGGTAACTACTCATGCTTTA	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11379_Pvir_Dia2013_COI	TSVA-034	1	BE11379_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	CTTAGTGGCATTAATTCGDAATCC	146.83376
BE11379	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25054	WGS84	BE11379	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11379_Rhar_Sim2018_COI	TSVA-034	1	BE11379_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11379	Sample	BE11379	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVA-034	COI	45	BE11379	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-034	CTGCTGCTATTGCTCATG	146.83376
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11380_Aamu_Bax2006_COI	TSVA-035	1	BE11380_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	GCACAACCGGGATCTTTACTTC	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11380_Pcan_Bot2011_IGS	TSVA-035	1	BE11380_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVA-035	IGS	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	CGTAATCCTCAGTACTGGCTA	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11380_Pper_Dia2013_COI	TSVA-035	1	BE11380_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Pper_Dia2013_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	CTTAGTGGCATTAATTCGDAATCC	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11380_Sspa_Oph2007_COI	TSVA-035	1	BE11380_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	GAAGGCCTTCCGATGATC	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11380_Upin_Bot2011_COI	TSVA-035	1	BE11380_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Upin_Bot2011_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	CTTTAATTACAGCGTTTTTATTGTTGT	146.83379
BE11380	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16038	-19.25085	WGS84	BE11380	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_A	28/11/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11380_Vgib_Wil2025_COI	TSVA-035	1	BE11380_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11380	Sample	BE11380	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVA-035	COI	45	BE11380	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVA-035	CCCCTCTTTACAAGTTCTTTAGGGT	146.83379
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11385_Asen_Wil2025_COI	GLTA-001	1	BE11385_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	GTTGTTGTTACAACACATGCTCTTTTG	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11385_Cjap_Sim2018_COI	GLTA-001	1	BE11385_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11385_Esin_And2018_Cyb	GLTA-001	1	BE11385_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-001	Cytb	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	ACCCCTCCTCATATCCAACCA	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11385_Mgig_Bot2012_COI	GLTA-001	1	BE11385_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	TCTTATTCGTTGGAGACTTTATAACCCT	151.32361
BE11385	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8565	WGS84	BE11385	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11385_Mjap_Gib2022_COI	GLTA-001	1	BE11385_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11385	Sample	BE11385	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-001	COI	45	BE11385	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-001	AATTTAATTGTGACTAGGCATGGTCTG	151.32361
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11386_Cmae_Bot2011_COI	GLTA-002	1	BE11386_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	ATGAACAGTCTATCCTCCTTTAG	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11386_Hsan_Wil2021_COI	GLTA-002	1	BE11386_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	CCTGGGCCGGTATAGTAGGT	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11386_Mros_Gib2022_COI	GLTA-002	1	BE11386_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11386_Msal_Bot2012_COI	GLTA-002	1	BE11386_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	GYTAGTTCCRATGATGTTAGCTG	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11386_Mstr_Wil2021_COI	GLTA-002	1	BE11386_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	GTAGTGGTAACTACTCATGCTTTA	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11386_Pvir_Dia2013_COI	GLTA-002	1	BE11386_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	CTTAGTGGCATTAATTCGDAATCC	151.32328
BE11386	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85594	WGS84	BE11386	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11386_Rhar_Sim2018_COI	GLTA-002	1	BE11386_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11386	Sample	BE11386	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-002	COI	45	BE11386	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-002	CTGCTGCTATTGCTCATG	151.32328
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11388_Aamu_Bax2006_COI	GLTA-004	1	BE11388_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	GCACAACCGGGATCTTTACTTC	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11388_Pcan_Bot2011_IGS	GLTA-004	1	BE11388_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-004	IGS	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	CGTAATCCTCAGTACTGGCTA	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11388_Pper_Dia2013_COI	GLTA-004	1	BE11388_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	CTTAGTGGCATTAATTCGDAATCC	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11388_Sspa_Oph2007_COI	GLTA-004	1	BE11388_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	GAAGGCCTTCCGATGATC	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11388_Upin_Bot2011_COI	GLTA-004	1	BE11388_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	CTTTAATTACAGCGTTTTTATTGTTGT	151.32448
BE11388	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85468	WGS84	BE11388	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11388_Vgib_Wil2025_COI	GLTA-004	1	BE11388_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11388	Sample	BE11388	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-004	COI	45	BE11388	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-004	CCCCTCTTTACAAGTTCTTTAGGGT	151.32448
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11389_Asen_Wil2025_COI	GLTA-005	1	BE11389_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	GTTGTTGTTACAACACATGCTCTTTTG	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11389_Cjap_Sim2018_COI	GLTA-005	1	BE11389_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11389_Esin_And2018_Cyb	GLTA-005	1	BE11389_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-005	Cytb	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	ACCCCTCCTCATATCCAACCA	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11389_Mgig_Bot2012_COI	GLTA-005	1	BE11389_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	TCTTATTCGTTGGAGACTTTATAACCCT	151.32614
BE11389	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85415	WGS84	BE11389	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11389_Mjap_Gib2022_COI	GLTA-005	1	BE11389_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11389	Sample	BE11389	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-005	COI	45	BE11389	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-005	AATTTAATTGTGACTAGGCATGGTCTG	151.32614
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11391_Aamu_Bax2006_COI	GLTA-007	1	BE11391_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	GCACAACCGGGATCTTTACTTC	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11391_Pcan_Bot2011_IGS	GLTA-007	1	BE11391_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-007	IGS	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	CGTAATCCTCAGTACTGGCTA	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11391_Pper_Dia2013_COI	GLTA-007	1	BE11391_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	CTTAGTGGCATTAATTCGDAATCC	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11391_Sspa_Oph2007_COI	GLTA-007	1	BE11391_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	GAAGGCCTTCCGATGATC	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11391_Upin_Bot2011_COI	GLTA-007	1	BE11391_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	CTTTAATTACAGCGTTTTTATTGTTGT	151.32495
BE11391	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85322	WGS84	BE11391	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11391_Vgib_Wil2025_COI	GLTA-007	1	BE11391_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11391	Sample	BE11391	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-007	COI	45	BE11391	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-007	CCCCTCTTTACAAGTTCTTTAGGGT	151.32495
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11392_Asen_Wil2025_COI	GLTA-008	1	BE11392_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	GTTGTTGTTACAACACATGCTCTTTTG	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11392_Cjap_Sim2018_COI	GLTA-008	1	BE11392_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11392_Esin_And2018_Cyb	GLTA-008	1	BE11392_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-008	Cytb	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	ACCCCTCCTCATATCCAACCA	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11392_Mgig_Bot2012_COI	GLTA-008	1	BE11392_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	TCTTATTCGTTGGAGACTTTATAACCCT	151.31684
BE11392	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85283	WGS84	BE11392	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11392_Mjap_Gib2022_COI	GLTA-008	1	BE11392_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11392	Sample	BE11392	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-008	COI	45	BE11392	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-008	AATTTAATTGTGACTAGGCATGGTCTG	151.31684
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11393_Cmae_Bot2011_COI	GLTA-009	1	BE11393_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	ATGAACAGTCTATCCTCCTTTAG	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11393_Hsan_Wil2021_COI	GLTA-009	1	BE11393_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	CCTGGGCCGGTATAGTAGGT	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11393_Mros_Gib2022_COI	GLTA-009	1	BE11393_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11393_Msal_Bot2012_COI	GLTA-009	1	BE11393_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	GYTAGTTCCRATGATGTTAGCTG	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11393_Mstr_Wil2021_COI	GLTA-009	1	BE11393_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	GTAGTGGTAACTACTCATGCTTTA	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11393_Pvir_Dia2013_COI	GLTA-009	1	BE11393_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	CTTAGTGGCATTAATTCGDAATCC	151.31842
BE11393	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85285	WGS84	BE11393	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11393_Rhar_Sim2018_COI	GLTA-009	1	BE11393_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11393	Sample	BE11393	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-009	COI	45	BE11393	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-009	CTGCTGCTATTGCTCATG	151.31842
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11395_Aamu_Bax2006_COI	GLTA-011	1	BE11395_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	GCACAACCGGGATCTTTACTTC	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11395_Pcan_Bot2011_IGS	GLTA-011	1	BE11395_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-011	IGS	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	CGTAATCCTCAGTACTGGCTA	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11395_Pper_Dia2013_COI	GLTA-011	1	BE11395_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	CTTAGTGGCATTAATTCGDAATCC	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11395_Sspa_Oph2007_COI	GLTA-011	1	BE11395_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	GAAGGCCTTCCGATGATC	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11395_Upin_Bot2011_COI	GLTA-011	1	BE11395_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	CTTTAATTACAGCGTTTTTATTGTTGT	151.32214
BE11395	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85203	WGS84	BE11395	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11395_Vgib_Wil2025_COI	GLTA-011	1	BE11395_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11395	Sample	BE11395	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-011	COI	45	BE11395	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-011	CCCCTCTTTACAAGTTCTTTAGGGT	151.32214
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11396_Asen_Wil2025_COI	GLTA-012	1	BE11396_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	GTTGTTGTTACAACACATGCTCTTTTG	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11396_Cjap_Sim2018_COI	GLTA-012	1	BE11396_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11396_Esin_And2018_Cyb	GLTA-012	1	BE11396_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-012	Cytb	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	ACCCCTCCTCATATCCAACCA	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11396_Mgig_Bot2012_COI	GLTA-012	1	BE11396_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	TCTTATTCGTTGGAGACTTTATAACCCT	151.32342
BE11396	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85135	WGS84	BE11396	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11396_Mjap_Gib2022_COI	GLTA-012	1	BE11396_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11396	Sample	BE11396	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-012	COI	45	BE11396	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-012	AATTTAATTGTGACTAGGCATGGTCTG	151.32342
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11397_Cmae_Bot2011_COI	GLTA-013	1	BE11397_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	ATGAACAGTCTATCCTCCTTTAG	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11397_Hsan_Wil2021_COI	GLTA-013	1	BE11397_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	CCTGGGCCGGTATAGTAGGT	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11397_Mros_Gib2022_COI	GLTA-013	1	BE11397_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11397_Msal_Bot2012_COI	GLTA-013	1	BE11397_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	GYTAGTTCCRATGATGTTAGCTG	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11397_Mstr_Wil2021_COI	GLTA-013	1	BE11397_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	GTAGTGGTAACTACTCATGCTTTA	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11397_Pvir_Dia2013_COI	GLTA-013	1	BE11397_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	CTTAGTGGCATTAATTCGDAATCC	151.3073
BE11397	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85269	WGS84	BE11397	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11397_Rhar_Sim2018_COI	GLTA-013	1	BE11397_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11397	Sample	BE11397	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-013	COI	45	BE11397	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-013	CTGCTGCTATTGCTCATG	151.3073
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11399_Aamu_Bax2006_COI	GLTA-015	1	BE11399_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	GCACAACCGGGATCTTTACTTC	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11399_Pcan_Bot2011_IGS	GLTA-015	1	BE11399_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-015	IGS	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	CGTAATCCTCAGTACTGGCTA	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11399_Pper_Dia2013_COI	GLTA-015	1	BE11399_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	CTTAGTGGCATTAATTCGDAATCC	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11399_Sspa_Oph2007_COI	GLTA-015	1	BE11399_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	GAAGGCCTTCCGATGATC	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11399_Upin_Bot2011_COI	GLTA-015	1	BE11399_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	CTTTAATTACAGCGTTTTTATTGTTGT	151.31768
BE11399	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8513	WGS84	BE11399	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11399_Vgib_Wil2025_COI	GLTA-015	1	BE11399_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11399	Sample	BE11399	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-015	COI	45	BE11399	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-015	CCCCTCTTTACAAGTTCTTTAGGGT	151.31768
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11400_Asen_Wil2025_COI	GLTA-016	1	BE11400_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	GTTGTTGTTACAACACATGCTCTTTTG	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11400_Cjap_Sim2018_COI	GLTA-016	1	BE11400_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11400_Esin_And2018_Cyb	GLTA-016	1	BE11400_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-016	Cytb	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	ACCCCTCCTCATATCCAACCA	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11400_Mgig_Bot2012_COI	GLTA-016	1	BE11400_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	TCTTATTCGTTGGAGACTTTATAACCCT	151.31914
BE11400	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85103	WGS84	BE11400	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11400_Mjap_Gib2022_COI	GLTA-016	1	BE11400_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11400	Sample	BE11400	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-016	COI	45	BE11400	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-016	AATTTAATTGTGACTAGGCATGGTCTG	151.31914
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11401_Cmae_Bot2011_COI	GLTA-017	1	BE11401_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	ATGAACAGTCTATCCTCCTTTAG	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11401_Mros_Gib2022_COI	GLTA-017	1	BE11401_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11401_Msal_Bot2012_COI	GLTA-017	1	BE11401_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	GYTAGTTCCRATGATGTTAGCTG	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11401_Mstr_Wil2021_COI	GLTA-017	1	BE11401_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	GTAGTGGTAACTACTCATGCTTTA	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11401_Pvir_Dia2013_COI	GLTA-017	1	BE11401_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	CTTAGTGGCATTAATTCGDAATCC	151.3209
BE11401	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85074	WGS84	BE11401	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11401_Rhar_Sim2018_COI	GLTA-017	1	BE11401_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11401	Sample	BE11401	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-017	COI	45	BE11401	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-017	CTGCTGCTATTGCTCATG	151.3209
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11403_Aamu_Bax2006_COI	GLTA-019	1	BE11403_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	GCACAACCGGGATCTTTACTTC	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11403_Pcan_Bot2011_IGS	GLTA-019	1	BE11403_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-019	IGS	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	CGTAATCCTCAGTACTGGCTA	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11403_Pper_Dia2013_COI	GLTA-019	1	BE11403_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	CTTAGTGGCATTAATTCGDAATCC	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11403_Sspa_Oph2007_COI	GLTA-019	1	BE11403_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	GAAGGCCTTCCGATGATC	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11403_Upin_Bot2011_COI	GLTA-019	1	BE11403_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	CTTTAATTACAGCGTTTTTATTGTTGT	151.30614
BE11403	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8515	WGS84	BE11403	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11403_Vgib_Wil2025_COI	GLTA-019	1	BE11403_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11403	Sample	BE11403	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-019	COI	45	BE11403	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-019	CCCCTCTTTACAAGTTCTTTAGGGT	151.30614
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11404_Asen_Wil2025_COI	GLTA-020	1	BE11404_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	GTTGTTGTTACAACACATGCTCTTTTG	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11404_Cjap_Sim2018_COI	GLTA-020	1	BE11404_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11404_Esin_And2018_Cyb	GLTA-020	1	BE11404_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-020	Cytb	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	ACCCCTCCTCATATCCAACCA	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11404_Mgig_Bot2012_COI	GLTA-020	1	BE11404_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	TCTTATTCGTTGGAGACTTTATAACCCT	151.308
BE11404	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85099	WGS84	BE11404	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11404_Mjap_Gib2022_COI	GLTA-020	1	BE11404_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11404	Sample	BE11404	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-020	COI	45	BE11404	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-020	AATTTAATTGTGACTAGGCATGGTCTG	151.308
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11405_Cmae_Bot2011_COI	GLTA-021	1	BE11405_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	ATGAACAGTCTATCCTCCTTTAG	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11405_Hsan_Wil2021_COI	GLTA-021	1	BE11405_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	CCTGGGCCGGTATAGTAGGT	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11405_Mros_Gib2022_COI	GLTA-021	1	BE11405_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11405_Msal_Bot2012_COI	GLTA-021	1	BE11405_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	GYTAGTTCCRATGATGTTAGCTG	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11405_Mstr_Wil2021_COI	GLTA-021	1	BE11405_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	GTAGTGGTAACTACTCATGCTTTA	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11405_Pvir_Dia2013_COI	GLTA-021	1	BE11405_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	CTTAGTGGCATTAATTCGDAATCC	151.30947
BE11405	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85082	WGS84	BE11405	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11405_Rhar_Sim2018_COI	GLTA-021	1	BE11405_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11405	Sample	BE11405	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-021	COI	45	BE11405	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-021	CTGCTGCTATTGCTCATG	151.30947
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11407_Aamu_Bax2006_COI	GLTA-023	1	BE11407_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	GCACAACCGGGATCTTTACTTC	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11407_Pcan_Bot2011_IGS	GLTA-023	1	BE11407_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-023	IGS	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	CGTAATCCTCAGTACTGGCTA	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11407_Pper_Dia2013_COI	GLTA-023	1	BE11407_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	CTTAGTGGCATTAATTCGDAATCC	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11407_Sspa_Oph2007_COI	GLTA-023	1	BE11407_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	GAAGGCCTTCCGATGATC	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11407_Upin_Bot2011_COI	GLTA-023	1	BE11407_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	CTTTAATTACAGCGTTTTTATTGTTGT	151.31293
BE11407	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85068	WGS84	BE11407	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11407_Vgib_Wil2025_COI	GLTA-023	1	BE11407_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11407	Sample	BE11407	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-023	COI	45	BE11407	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-023	CCCCTCTTTACAAGTTCTTTAGGGT	151.31293
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11408_Asen_Wil2025_COI	GLTA-024	1	BE11408_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	GTTGTTGTTACAACACATGCTCTTTTG	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11408_Cjap_Sim2018_COI	GLTA-024	1	BE11408_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11408_Esin_And2018_Cyb	GLTA-024	1	BE11408_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-024	Cytb	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	ACCCCTCCTCATATCCAACCA	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11408_Mgig_Bot2012_COI	GLTA-024	1	BE11408_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	TCTTATTCGTTGGAGACTTTATAACCCT	151.31454
BE11408	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85048	WGS84	BE11408	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11408_Mjap_Gib2022_COI	GLTA-024	1	BE11408_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11408	Sample	BE11408	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-024	COI	45	BE11408	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-024	AATTTAATTGTGACTAGGCATGGTCTG	151.31454
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11409_Cmae_Bot2011_COI	GLTA-025	1	BE11409_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	ATGAACAGTCTATCCTCCTTTAG	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11409_Hsan_Wil2021_COI	GLTA-025	1	BE11409_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	CCTGGGCCGGTATAGTAGGT	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11409_Mros_Gib2022_COI	GLTA-025	1	BE11409_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11409_Msal_Bot2012_COI	GLTA-025	1	BE11409_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	GYTAGTTCCRATGATGTTAGCTG	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11409_Mstr_Wil2021_COI	GLTA-025	1	BE11409_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	GTAGTGGTAACTACTCATGCTTTA	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11409_Pvir_Dia2013_COI	GLTA-025	1	BE11409_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	CTTAGTGGCATTAATTCGDAATCC	151.3163
BE11409	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85021	WGS84	BE11409	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11409_Rhar_Sim2018_COI	GLTA-025	1	BE11409_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11409	Sample	BE11409	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-025	COI	45	BE11409	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-025	CTGCTGCTATTGCTCATG	151.3163
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11410_Aamu_Bax2006_COI	GLTA-026	1	BE11410_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	GCACAACCGGGATCTTTACTTC	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11410_Pcan_Bot2011_IGS	GLTA-026	1	BE11410_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-026	IGS	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	CGTAATCCTCAGTACTGGCTA	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11410_Pper_Dia2013_COI	GLTA-026	1	BE11410_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	CTTAGTGGCATTAATTCGDAATCC	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11410_Sspa_Oph2007_COI	GLTA-026	1	BE11410_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	GAAGGCCTTCCGATGATC	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11410_Upin_Bot2011_COI	GLTA-026	1	BE11410_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	CTTTAATTACAGCGTTTTTATTGTTGT	151.31826
BE11410	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84992	WGS84	BE11410	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11410_Vgib_Wil2025_COI	GLTA-026	1	BE11410_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11410	Sample	BE11410	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-026	COI	45	BE11410	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-026	CCCCTCTTTACAAGTTCTTTAGGGT	151.31826
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11411_Asen_Wil2025_COI	GLTA-027	1	BE11411_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	GTTGTTGTTACAACACATGCTCTTTTG	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11411_Cjap_Sim2018_COI	GLTA-027	1	BE11411_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11411_Esin_And2018_Cyb	GLTA-027	1	BE11411_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-027	Cytb	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	ACCCCTCCTCATATCCAACCA	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11411_Mgig_Bot2012_COI	GLTA-027	1	BE11411_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	TCTTATTCGTTGGAGACTTTATAACCCT	151.31994
BE11411	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84952	WGS84	BE11411	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11411_Mjap_Gib2022_COI	GLTA-027	1	BE11411_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11411	Sample	BE11411	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-027	COI	45	BE11411	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-027	AATTTAATTGTGACTAGGCATGGTCTG	151.31994
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11412_Cmae_Bot2011_COI	GLTA-028	1	BE11412_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	ATGAACAGTCTATCCTCCTTTAG	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11412_Hsan_Wil2021_COI	GLTA-028	1	BE11412_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	CCTGGGCCGGTATAGTAGGT	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11412_Mros_Gib2022_COI	GLTA-028	1	BE11412_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11412_Msal_Bot2012_COI	GLTA-028	1	BE11412_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	GYTAGTTCCRATGATGTTAGCTG	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11412_Mstr_Wil2021_COI	GLTA-028	1	BE11412_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	GTAGTGGTAACTACTCATGCTTTA	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11412_Pvir_Dia2013_COI	GLTA-028	1	BE11412_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	CTTAGTGGCATTAATTCGDAATCC	151.30704
BE11412	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.85004	WGS84	BE11412	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11412_Rhar_Sim2018_COI	GLTA-028	1	BE11412_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11412	Sample	BE11412	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-028	COI	45	BE11412	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-028	CTGCTGCTATTGCTCATG	151.30704
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11414_Aamu_Bax2006_COI	GLTA-030	1	BE11414_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	GCACAACCGGGATCTTTACTTC	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11414_Pcan_Bot2011_IGS	GLTA-030	1	BE11414_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-030	IGS	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	CGTAATCCTCAGTACTGGCTA	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11414_Pper_Dia2013_COI	GLTA-030	1	BE11414_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	CTTAGTGGCATTAATTCGDAATCC	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11414_Sspa_Oph2007_COI	GLTA-030	1	BE11414_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	GAAGGCCTTCCGATGATC	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11414_Upin_Bot2011_COI	GLTA-030	1	BE11414_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	CTTTAATTACAGCGTTTTTATTGTTGT	151.31051
BE11414	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84958	WGS84	BE11414	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11414_Vgib_Wil2025_COI	GLTA-030	1	BE11414_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11414	Sample	BE11414	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-030	COI	45	BE11414	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-030	CCCCTCTTTACAAGTTCTTTAGGGT	151.31051
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11415_Asen_Wil2025_COI	GLTA-031	1	BE11415_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	GTTGTTGTTACAACACATGCTCTTTTG	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11415_Cjap_Sim2018_COI	GLTA-031	1	BE11415_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11415_Esin_And2018_Cyb	GLTA-031	1	BE11415_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-031	Cytb	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	ACCCCTCCTCATATCCAACCA	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11415_Mgig_Bot2012_COI	GLTA-031	1	BE11415_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	TCTTATTCGTTGGAGACTTTATAACCCT	151.3121
BE11415	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84931	WGS84	BE11415	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11415_Mjap_Gib2022_COI	GLTA-031	1	BE11415_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11415	Sample	BE11415	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-031	COI	45	BE11415	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-031	AATTTAATTGTGACTAGGCATGGTCTG	151.3121
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11416_Cmae_Bot2011_COI	GLTA-032	1	BE11416_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	ATGAACAGTCTATCCTCCTTTAG	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11416_Mros_Gib2022_COI	GLTA-032	1	BE11416_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11416_Msal_Bot2012_COI	GLTA-032	1	BE11416_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	GYTAGTTCCRATGATGTTAGCTG	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11416_Mstr_Wil2021_COI	GLTA-032	1	BE11416_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	GTAGTGGTAACTACTCATGCTTTA	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11416_Pvir_Dia2013_COI	GLTA-032	1	BE11416_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	CTTAGTGGCATTAATTCGDAATCC	151.31387
BE11416	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84927	WGS84	BE11416	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11416_Rhar_Sim2018_COI	GLTA-032	1	BE11416_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11416	Sample	BE11416	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-032	COI	45	BE11416	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-032	CTGCTGCTATTGCTCATG	151.31387
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11418_Aamu_Bax2006_COI	GLTA-034	1	BE11418_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	GCACAACCGGGATCTTTACTTC	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11418_Pcan_Bot2011_IGS	GLTA-034	1	BE11418_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-034	IGS	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	CGTAATCCTCAGTACTGGCTA	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11418_Pper_Dia2013_COI	GLTA-034	1	BE11418_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	CTTAGTGGCATTAATTCGDAATCC	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11418_Sspa_Oph2007_COI	GLTA-034	1	BE11418_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	GAAGGCCTTCCGATGATC	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11418_Upin_Bot2011_COI	GLTA-034	1	BE11418_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	CTTTAATTACAGCGTTTTTATTGTTGT	151.31106
BE11418	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84814	WGS84	BE11418	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11418_Vgib_Wil2025_COI	GLTA-034	1	BE11418_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11418	Sample	BE11418	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-034	COI	45	BE11418	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-034	CCCCTCTTTACAAGTTCTTTAGGGT	151.31106
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11419_Asen_Wil2025_COI	GLTA-035	1	BE11419_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	GTTGTTGTTACAACACATGCTCTTTTG	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11419_Cjap_Sim2018_COI	GLTA-035	1	BE11419_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11419_Esin_And2018_Cyb	GLTA-035	1	BE11419_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-035	Cytb	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	ACCCCTCCTCATATCCAACCA	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11419_Mgig_Bot2012_COI	GLTA-035	1	BE11419_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	TCTTATTCGTTGGAGACTTTATAACCCT	151.31275
BE11419	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.84779	WGS84	BE11419	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_A	20/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11419_Mjap_Gib2022_COI	GLTA-035	1	BE11419_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11419	Sample	BE11419	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-035	COI	45	BE11419	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-035	AATTTAATTGTGACTAGGCATGGTCTG	151.31275
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11425_Aamu_Bax2006_COI	GLTA-037	1	BE11425_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	GCACAACCGGGATCTTTACTTC	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11425_Pcan_Bot2011_IGS	GLTA-037	1	BE11425_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-037	IGS	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	CGTAATCCTCAGTACTGGCTA	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11425_Pper_Dia2013_COI	GLTA-037	1	BE11425_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	CTTAGTGGCATTAATTCGDAATCC	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11425_Sspa_Oph2007_COI	GLTA-037	1	BE11425_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	GAAGGCCTTCCGATGATC	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11425_Upin_Bot2011_COI	GLTA-037	1	BE11425_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	CTTTAATTACAGCGTTTTTATTGTTGT	151.27401
BE11425	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83811	WGS84	BE11425	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11425_Vgib_Wil2025_COI	GLTA-037	1	BE11425_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11425	Sample	BE11425	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-037	COI	45	BE11425	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-037	CCCCTCTTTACAAGTTCTTTAGGGT	151.27401
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11426_Asen_Wil2025_COI	GLTA-038	1	BE11426_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	GTTGTTGTTACAACACATGCTCTTTTG	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11426_Cjap_Sim2018_COI	GLTA-038	1	BE11426_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11426_Esin_And2018_Cyb	GLTA-038	1	BE11426_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-038	Cytb	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	ACCCCTCCTCATATCCAACCA	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11426_Mgig_Bot2012_COI	GLTA-038	1	BE11426_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	TCTTATTCGTTGGAGACTTTATAACCCT	151.27728
BE11426	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83778	WGS84	BE11426	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11426_Mjap_Gib2022_COI	GLTA-038	1	BE11426_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11426	Sample	BE11426	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-038	COI	45	BE11426	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-038	AATTTAATTGTGACTAGGCATGGTCTG	151.27728
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11427_Cmae_Bot2011_COI	GLTA-039	1	BE11427_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	ATGAACAGTCTATCCTCCTTTAG	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11427_Hsan_Wil2021_COI	GLTA-039	1	BE11427_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	CCTGGGCCGGTATAGTAGGT	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11427_Mros_Gib2022_COI	GLTA-039	1	BE11427_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11427_Msal_Bot2012_COI	GLTA-039	1	BE11427_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	GYTAGTTCCRATGATGTTAGCTG	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11427_Mstr_Wil2021_COI	GLTA-039	1	BE11427_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	GTAGTGGTAACTACTCATGCTTTA	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11427_Pvir_Dia2013_COI	GLTA-039	1	BE11427_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	CTTAGTGGCATTAATTCGDAATCC	151.27652
BE11427	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83655	WGS84	BE11427	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11427_Rhar_Sim2018_COI	GLTA-039	1	BE11427_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11427	Sample	BE11427	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-039	COI	45	BE11427	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-039	CTGCTGCTATTGCTCATG	151.27652
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11429_Aamu_Bax2006_COI	GLTA-041	1	BE11429_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	GCACAACCGGGATCTTTACTTC	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11429_Pcan_Bot2011_IGS	GLTA-041	1	BE11429_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-041	IGS	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	CGTAATCCTCAGTACTGGCTA	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11429_Pper_Dia2013_COI	GLTA-041	1	BE11429_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	CTTAGTGGCATTAATTCGDAATCC	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11429_Sspa_Oph2007_COI	GLTA-041	1	BE11429_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	GAAGGCCTTCCGATGATC	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11429_Upin_Bot2011_COI	GLTA-041	1	BE11429_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	CTTTAATTACAGCGTTTTTATTGTTGT	151.27704
BE11429	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83475	WGS84	BE11429	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11429_Vgib_Wil2025_COI	GLTA-041	1	BE11429_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11429	Sample	BE11429	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-041	COI	45	BE11429	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-041	CCCCTCTTTACAAGTTCTTTAGGGT	151.27704
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11430_Cmae_Bot2011_COI	GLTA-042	1	BE11430_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	ATGAACAGTCTATCCTCCTTTAG	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11430_Hsan_Wil2021_COI	GLTA-042	1	BE11430_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	CCTGGGCCGGTATAGTAGGT	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11430_Mros_Gib2022_COI	GLTA-042	1	BE11430_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11430_Msal_Bot2012_COI	GLTA-042	1	BE11430_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	GYTAGTTCCRATGATGTTAGCTG	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11430_Mstr_Wil2021_COI	GLTA-042	1	BE11430_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	GTAGTGGTAACTACTCATGCTTTA	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11430_Pvir_Dia2013_COI	GLTA-042	1	BE11430_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	CTTAGTGGCATTAATTCGDAATCC	151.26694
BE11430	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83435	WGS84	BE11430	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11430_Rhar_Sim2018_COI	GLTA-042	1	BE11430_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11430	Sample	BE11430	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-042	COI	45	BE11430	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-042	CTGCTGCTATTGCTCATG	151.26694
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11432_Aamu_Bax2006_COI	GLTA-044	1	BE11432_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	GCACAACCGGGATCTTTACTTC	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11432_Pcan_Bot2011_IGS	GLTA-044	1	BE11432_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-044	IGS	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	CGTAATCCTCAGTACTGGCTA	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11432_Pper_Dia2013_COI	GLTA-044	1	BE11432_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	CTTAGTGGCATTAATTCGDAATCC	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11432_Sspa_Oph2007_COI	GLTA-044	1	BE11432_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	GAAGGCCTTCCGATGATC	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11432_Upin_Bot2011_COI	GLTA-044	1	BE11432_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	CTTTAATTACAGCGTTTTTATTGTTGT	151.27076
BE11432	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83382	WGS84	BE11432	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11432_Vgib_Wil2025_COI	GLTA-044	1	BE11432_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11432	Sample	BE11432	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-044	COI	45	BE11432	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-044	CCCCTCTTTACAAGTTCTTTAGGGT	151.27076
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11433_Asen_Wil2025_COI	GLTA-045	1	BE11433_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	GTTGTTGTTACAACACATGCTCTTTTG	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11433_Cjap_Sim2018_COI	GLTA-045	1	BE11433_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11433_Esin_And2018_Cyb	GLTA-045	1	BE11433_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-045	Cytb	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	ACCCCTCCTCATATCCAACCA	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11433_Mgig_Bot2012_COI	GLTA-045	1	BE11433_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	TCTTATTCGTTGGAGACTTTATAACCCT	151.27266
BE11433	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83387	WGS84	BE11433	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11433_Mjap_Gib2022_COI	GLTA-045	1	BE11433_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11433	Sample	BE11433	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-045	COI	45	BE11433	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-045	AATTTAATTGTGACTAGGCATGGTCTG	151.27266
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11434_Cmae_Bot2011_COI	GLTA-046	1	BE11434_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	ATGAACAGTCTATCCTCCTTTAG	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11434_Mros_Gib2022_COI	GLTA-046	1	BE11434_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11434_Msal_Bot2012_COI	GLTA-046	1	BE11434_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	GYTAGTTCCRATGATGTTAGCTG	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11434_Mstr_Wil2021_COI	GLTA-046	1	BE11434_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	GTAGTGGTAACTACTCATGCTTTA	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11434_Pvir_Dia2013_COI	GLTA-046	1	BE11434_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	CTTAGTGGCATTAATTCGDAATCC	151.27417
BE11434	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11434	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11434_Rhar_Sim2018_COI	GLTA-046	1	BE11434_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11434	Sample	BE11434	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-046	COI	45	BE11434	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-046	CTGCTGCTATTGCTCATG	151.27417
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11436_Aamu_Bax2006_COI	GLTA-048	1	BE11436_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	GCACAACCGGGATCTTTACTTC	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11436_Pcan_Bot2011_IGS	GLTA-048	1	BE11436_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-048	IGS	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	CGTAATCCTCAGTACTGGCTA	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11436_Pper_Dia2013_COI	GLTA-048	1	BE11436_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	CTTAGTGGCATTAATTCGDAATCC	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11436_Sspa_Oph2007_COI	GLTA-048	1	BE11436_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	GAAGGCCTTCCGATGATC	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11436_Upin_Bot2011_COI	GLTA-048	1	BE11436_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	CTTTAATTACAGCGTTTTTATTGTTGT	151.26603
BE11436	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83333	WGS84	BE11436	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11436_Vgib_Wil2025_COI	GLTA-048	1	BE11436_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11436	Sample	BE11436	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-048	COI	45	BE11436	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-048	CCCCTCTTTACAAGTTCTTTAGGGT	151.26603
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11437_Asen_Wil2025_COI	GLTA-049	1	BE11437_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	GTTGTTGTTACAACACATGCTCTTTTG	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11437_Cjap_Sim2018_COI	GLTA-049	1	BE11437_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11437_Esin_And2018_Cyb	GLTA-049	1	BE11437_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-049	Cytb	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	ACCCCTCCTCATATCCAACCA	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11437_Mgig_Bot2012_COI	GLTA-049	1	BE11437_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	TCTTATTCGTTGGAGACTTTATAACCCT	151.26763
BE11437	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8331	WGS84	BE11437	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11437_Mjap_Gib2022_COI	GLTA-049	1	BE11437_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11437	Sample	BE11437	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-049	COI	45	BE11437	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-049	AATTTAATTGTGACTAGGCATGGTCTG	151.26763
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11438_Cmae_Bot2011_COI	GLTA-050	1	BE11438_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	ATGAACAGTCTATCCTCCTTTAG	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11438_Hsan_Wil2021_COI	GLTA-050	1	BE11438_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	CCTGGGCCGGTATAGTAGGT	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11438_Mros_Gib2022_COI	GLTA-050	1	BE11438_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11438_Msal_Bot2012_COI	GLTA-050	1	BE11438_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	GYTAGTTCCRATGATGTTAGCTG	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11438_Mstr_Wil2021_COI	GLTA-050	1	BE11438_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	GTAGTGGTAACTACTCATGCTTTA	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11438_Pvir_Dia2013_COI	GLTA-050	1	BE11438_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	CTTAGTGGCATTAATTCGDAATCC	151.26955
BE11438	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83272	WGS84	BE11438	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11438_Rhar_Sim2018_COI	GLTA-050	1	BE11438_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11438	Sample	BE11438	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-050	COI	45	BE11438	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-050	CTGCTGCTATTGCTCATG	151.26955
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11440_Asen_Wil2025_COI	GLTA-052	1	BE11440_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	GTTGTTGTTACAACACATGCTCTTTTG	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11440_Cjap_Sim2018_COI	GLTA-052	1	BE11440_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11440_Esin_And2018_Cyb	GLTA-052	1	BE11440_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-052	Cytb	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	ACCCCTCCTCATATCCAACCA	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11440_Mgig_Bot2012_COI	GLTA-052	1	BE11440_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	TCTTATTCGTTGGAGACTTTATAACCCT	151.27336
BE11440	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83215	WGS84	BE11440	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11440_Mjap_Gib2022_COI	GLTA-052	1	BE11440_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11440	Sample	BE11440	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-052	COI	45	BE11440	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-052	AATTTAATTGTGACTAGGCATGGTCTG	151.27336
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11441_Cmae_Bot2011_COI	GLTA-053	1	BE11441_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	ATGAACAGTCTATCCTCCTTTAG	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11441_Hsan_Wil2021_COI	GLTA-053	1	BE11441_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	CCTGGGCCGGTATAGTAGGT	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11441_Mros_Gib2022_COI	GLTA-053	1	BE11441_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11441_Msal_Bot2012_COI	GLTA-053	1	BE11441_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	GYTAGTTCCRATGATGTTAGCTG	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11441_Mstr_Wil2021_COI	GLTA-053	1	BE11441_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	GTAGTGGTAACTACTCATGCTTTA	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11441_Pvir_Dia2013_COI	GLTA-053	1	BE11441_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	CTTAGTGGCATTAATTCGDAATCC	151.26337
BE11441	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83191	WGS84	BE11441	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11441_Rhar_Sim2018_COI	GLTA-053	1	BE11441_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11441	Sample	BE11441	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-053	COI	45	BE11441	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-053	CTGCTGCTATTGCTCATG	151.26337
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11443_Aamu_Bax2006_COI	GLTA-055	1	BE11443_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	GCACAACCGGGATCTTTACTTC	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11443_Pcan_Bot2011_IGS	GLTA-055	1	BE11443_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-055	IGS	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	CGTAATCCTCAGTACTGGCTA	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11443_Pper_Dia2013_COI	GLTA-055	1	BE11443_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	CTTAGTGGCATTAATTCGDAATCC	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11443_Sspa_Oph2007_COI	GLTA-055	1	BE11443_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	GAAGGCCTTCCGATGATC	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11443_Upin_Bot2011_COI	GLTA-055	1	BE11443_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	CTTTAATTACAGCGTTTTTATTGTTGT	151.26634
BE11443	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83181	WGS84	BE11443	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11443_Vgib_Wil2025_COI	GLTA-055	1	BE11443_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11443	Sample	BE11443	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-055	COI	45	BE11443	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-055	CCCCTCTTTACAAGTTCTTTAGGGT	151.26634
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11444_Asen_Wil2025_COI	GLTA-056	1	BE11444_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	GTTGTTGTTACAACACATGCTCTTTTG	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11444_Cjap_Sim2018_COI	GLTA-056	1	BE11444_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11444_Esin_And2018_Cyb	GLTA-056	1	BE11444_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-056	Cytb	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	ACCCCTCCTCATATCCAACCA	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11444_Mgig_Bot2012_COI	GLTA-056	1	BE11444_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	TCTTATTCGTTGGAGACTTTATAACCCT	151.26801
BE11444	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83172	WGS84	BE11444	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11444_Mjap_Gib2022_COI	GLTA-056	1	BE11444_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11444	Sample	BE11444	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-056	COI	45	BE11444	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-056	AATTTAATTGTGACTAGGCATGGTCTG	151.26801
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11445_Cmae_Bot2011_COI	GLTA-057	1	BE11445_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	ATGAACAGTCTATCCTCCTTTAG	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11445_Hsan_Wil2021_COI	GLTA-057	1	BE11445_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	CCTGGGCCGGTATAGTAGGT	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11445_Mros_Gib2022_COI	GLTA-057	1	BE11445_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11445_Msal_Bot2012_COI	GLTA-057	1	BE11445_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	GYTAGTTCCRATGATGTTAGCTG	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11445_Mstr_Wil2021_COI	GLTA-057	1	BE11445_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	GTAGTGGTAACTACTCATGCTTTA	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11445_Pvir_Dia2013_COI	GLTA-057	1	BE11445_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	CTTAGTGGCATTAATTCGDAATCC	151.25797
BE11445	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83136	WGS84	BE11445	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11445_Rhar_Sim2018_COI	GLTA-057	1	BE11445_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11445	Sample	BE11445	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-057	COI	45	BE11445	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-057	CTGCTGCTATTGCTCATG	151.25797
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11447_Aamu_Bax2006_COI	GLTA-059	1	BE11447_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	GCACAACCGGGATCTTTACTTC	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11447_Pcan_Bot2011_IGS	GLTA-059	1	BE11447_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-059	IGS	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	CGTAATCCTCAGTACTGGCTA	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11447_Pper_Dia2013_COI	GLTA-059	1	BE11447_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	CTTAGTGGCATTAATTCGDAATCC	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11447_Sspa_Oph2007_COI	GLTA-059	1	BE11447_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	GAAGGCCTTCCGATGATC	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11447_Upin_Bot2011_COI	GLTA-059	1	BE11447_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	CTTTAATTACAGCGTTTTTATTGTTGT	151.26129
BE11447	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83022	WGS84	BE11447	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11447_Vgib_Wil2025_COI	GLTA-059	1	BE11447_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11447	Sample	BE11447	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-059	COI	45	BE11447	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-059	CCCCTCTTTACAAGTTCTTTAGGGT	151.26129
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11448_Asen_Wil2025_COI	GLTA-060	1	BE11448_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	GTTGTTGTTACAACACATGCTCTTTTG	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11448_Cjap_Sim2018_COI	GLTA-060	1	BE11448_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11448_Esin_And2018_Cyb	GLTA-060	1	BE11448_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-060	Cytb	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	ACCCCTCCTCATATCCAACCA	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11448_Mgig_Bot2012_COI	GLTA-060	1	BE11448_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	TCTTATTCGTTGGAGACTTTATAACCCT	151.26294
BE11448	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.83018	WGS84	BE11448	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11448_Mjap_Gib2022_COI	GLTA-060	1	BE11448_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11448	Sample	BE11448	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-060	COI	45	BE11448	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-060	AATTTAATTGTGACTAGGCATGGTCTG	151.26294
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11449_Cmae_Bot2011_COI	GLTA-061	1	BE11449_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	ATGAACAGTCTATCCTCCTTTAG	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11449_Hsan_Wil2021_COI	GLTA-061	1	BE11449_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	CCTGGGCCGGTATAGTAGGT	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11449_Mros_Gib2022_COI	GLTA-061	1	BE11449_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11449_Msal_Bot2012_COI	GLTA-061	1	BE11449_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	GYTAGTTCCRATGATGTTAGCTG	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11449_Mstr_Wil2021_COI	GLTA-061	1	BE11449_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	GTAGTGGTAACTACTCATGCTTTA	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11449_Pvir_Dia2013_COI	GLTA-061	1	BE11449_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	CTTAGTGGCATTAATTCGDAATCC	151.26484
BE11449	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82988	WGS84	BE11449	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11449_Rhar_Sim2018_COI	GLTA-061	1	BE11449_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11449	Sample	BE11449	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-061	COI	45	BE11449	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-061	CTGCTGCTATTGCTCATG	151.26484
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11450_Aamu_Bax2006_COI	GLTA-062	1	BE11450_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	GCACAACCGGGATCTTTACTTC	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11450_Pcan_Bot2011_IGS	GLTA-062	1	BE11450_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-062	IGS	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	CGTAATCCTCAGTACTGGCTA	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11450_Pper_Dia2013_COI	GLTA-062	1	BE11450_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	CTTAGTGGCATTAATTCGDAATCC	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11450_Sspa_Oph2007_COI	GLTA-062	1	BE11450_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	GAAGGCCTTCCGATGATC	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11450_Upin_Bot2011_COI	GLTA-062	1	BE11450_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	CTTTAATTACAGCGTTTTTATTGTTGT	151.25286
BE11450	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82995	WGS84	BE11450	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11450_Vgib_Wil2025_COI	GLTA-062	1	BE11450_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11450	Sample	BE11450	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-062	COI	45	BE11450	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-062	CCCCTCTTTACAAGTTCTTTAGGGT	151.25286
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11451_Asen_Wil2025_COI	GLTA-063	1	BE11451_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	GTTGTTGTTACAACACATGCTCTTTTG	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11451_Cjap_Sim2018_COI	GLTA-063	1	BE11451_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11451_Esin_And2018_Cyb	GLTA-063	1	BE11451_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-063	Cytb	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	ACCCCTCCTCATATCCAACCA	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11451_Mgig_Bot2012_COI	GLTA-063	1	BE11451_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	TCTTATTCGTTGGAGACTTTATAACCCT	151.25459
BE11451	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82966	WGS84	BE11451	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11451_Mjap_Gib2022_COI	GLTA-063	1	BE11451_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11451	Sample	BE11451	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-063	COI	45	BE11451	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-063	AATTTAATTGTGACTAGGCATGGTCTG	151.25459
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11452_Cmae_Bot2011_COI	GLTA-064	1	BE11452_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	ATGAACAGTCTATCCTCCTTTAG	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11452_Hsan_Wil2021_COI	GLTA-064	1	BE11452_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	CCTGGGCCGGTATAGTAGGT	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11452_Mros_Gib2022_COI	GLTA-064	1	BE11452_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11452_Msal_Bot2012_COI	GLTA-064	1	BE11452_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	GYTAGTTCCRATGATGTTAGCTG	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11452_Mstr_Wil2021_COI	GLTA-064	1	BE11452_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	GTAGTGGTAACTACTCATGCTTTA	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11452_Pvir_Dia2013_COI	GLTA-064	1	BE11452_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	CTTAGTGGCATTAATTCGDAATCC	151.25594
BE11452	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82962	WGS84	BE11452	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11452_Rhar_Sim2018_COI	GLTA-064	1	BE11452_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11452	Sample	BE11452	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-064	COI	45	BE11452	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-064	CTGCTGCTATTGCTCATG	151.25594
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11454_Aamu_Bax2006_COI	GLTA-066	1	BE11454_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	GCACAACCGGGATCTTTACTTC	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11454_Pcan_Bot2011_IGS	GLTA-066	1	BE11454_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-066	IGS	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	CGTAATCCTCAGTACTGGCTA	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11454_Pper_Dia2013_COI	GLTA-066	1	BE11454_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	CTTAGTGGCATTAATTCGDAATCC	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11454_Sspa_Oph2007_COI	GLTA-066	1	BE11454_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	GAAGGCCTTCCGATGATC	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11454_Upin_Bot2011_COI	GLTA-066	1	BE11454_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	CTTTAATTACAGCGTTTTTATTGTTGT	151.26022
BE11454	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82888	WGS84	BE11454	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11454_Vgib_Wil2025_COI	GLTA-066	1	BE11454_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11454	Sample	BE11454	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-066	COI	45	BE11454	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-066	CCCCTCTTTACAAGTTCTTTAGGGT	151.26022
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11455_Asen_Wil2025_COI	GLTA-067	1	BE11455_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	GTTGTTGTTACAACACATGCTCTTTTG	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11455_Cjap_Sim2018_COI	GLTA-067	1	BE11455_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11455_Esin_And2018_Cyb	GLTA-067	1	BE11455_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-067	Cytb	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	ACCCCTCCTCATATCCAACCA	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11455_Mgig_Bot2012_COI	GLTA-067	1	BE11455_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	TCTTATTCGTTGGAGACTTTATAACCCT	151.26156
BE11455	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82955	WGS84	BE11455	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11455_Mjap_Gib2022_COI	GLTA-067	1	BE11455_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11455	Sample	BE11455	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-067	COI	45	BE11455	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-067	AATTTAATTGTGACTAGGCATGGTCTG	151.26156
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11456_Cmae_Bot2011_COI	GLTA-068	1	BE11456_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	ATGAACAGTCTATCCTCCTTTAG	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11456_Hsan_Wil2021_COI	GLTA-068	1	BE11456_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	CCTGGGCCGGTATAGTAGGT	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11456_Mros_Gib2022_COI	GLTA-068	1	BE11456_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11456_Msal_Bot2012_COI	GLTA-068	1	BE11456_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	GYTAGTTCCRATGATGTTAGCTG	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11456_Mstr_Wil2021_COI	GLTA-068	1	BE11456_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	GTAGTGGTAACTACTCATGCTTTA	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11456_Pvir_Dia2013_COI	GLTA-068	1	BE11456_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	CTTAGTGGCATTAATTCGDAATCC	151.25335
BE11456	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.8284	WGS84	BE11456	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11456_Rhar_Sim2018_COI	GLTA-068	1	BE11456_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11456	Sample	BE11456	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-068	COI	45	BE11456	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-068	CTGCTGCTATTGCTCATG	151.25335
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11458_Aamu_Bax2006_COI	GLTA-070	1	BE11458_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	GCACAACCGGGATCTTTACTTC	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11458_Pcan_Bot2011_IGS	GLTA-070	1	BE11458_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-070	IGS	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	CGTAATCCTCAGTACTGGCTA	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11458_Pper_Dia2013_COI	GLTA-070	1	BE11458_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	CTTAGTGGCATTAATTCGDAATCC	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11458_Sspa_Oph2007_COI	GLTA-070	1	BE11458_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	GAAGGCCTTCCGATGATC	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11458_Upin_Bot2011_COI	GLTA-070	1	BE11458_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	CTTTAATTACAGCGTTTTTATTGTTGT	151.25701
BE11458	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16040	-23.82805	WGS84	BE11458	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_A	23/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11458_Vgib_Wil2025_COI	GLTA-070	1	BE11458_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11458	Sample	BE11458	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-070	COI	45	BE11458	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-070	CCCCTCTTTACAAGTTCTTTAGGGT	151.25701
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11463_Cmae_Bot2011_COI	GLTA-076	1	BE11463_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	ATGAACAGTCTATCCTCCTTTAG	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11463_Hsan_Wil2021_COI	GLTA-076	1	BE11463_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	CCTGGGCCGGTATAGTAGGT	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11463_Mros_Gib2022_COI	GLTA-076	1	BE11463_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11463_Msal_Bot2012_COI	GLTA-076	1	BE11463_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	GYTAGTTCCRATGATGTTAGCTG	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11463_Mstr_Wil2021_COI	GLTA-076	1	BE11463_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	GTAGTGGTAACTACTCATGCTTTA	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11463_Pvir_Dia2013_COI	GLTA-076	1	BE11463_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	CTTAGTGGCATTAATTCGDAATCC	151.18532
BE11463	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79361	WGS84	BE11463	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11463_Rhar_Sim2018_COI	GLTA-076	1	BE11463_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11463	Sample	BE11463	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-076	COI	45	BE11463	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-076	CTGCTGCTATTGCTCATG	151.18532
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11465_Aamu_Bax2006_COI	GLTA-078	1	BE11465_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	GCACAACCGGGATCTTTACTTC	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11465_Pcan_Bot2011_IGS	GLTA-078	1	BE11465_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-078	IGS	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	CGTAATCCTCAGTACTGGCTA	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11465_Pper_Dia2013_COI	GLTA-078	1	BE11465_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	CTTAGTGGCATTAATTCGDAATCC	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11465_Sspa_Oph2007_COI	GLTA-078	1	BE11465_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	GAAGGCCTTCCGATGATC	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11465_Upin_Bot2011_COI	GLTA-078	1	BE11465_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	CTTTAATTACAGCGTTTTTATTGTTGT	151.18864
BE11465	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79297	WGS84	BE11465	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11465_Vgib_Wil2025_COI	GLTA-078	1	BE11465_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11465	Sample	BE11465	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-078	COI	45	BE11465	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-078	CCCCTCTTTACAAGTTCTTTAGGGT	151.18864
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11466_Asen_Wil2025_COI	GLTA-079	1	BE11466_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	GTTGTTGTTACAACACATGCTCTTTTG	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11466_Cjap_Sim2018_COI	GLTA-079	1	BE11466_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11466_Esin_And2018_Cyb	GLTA-079	1	BE11466_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-079	Cytb	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	ACCCCTCCTCATATCCAACCA	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11466_Mgig_Bot2012_COI	GLTA-079	1	BE11466_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	TCTTATTCGTTGGAGACTTTATAACCCT	151.18113
BE11466	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79294	WGS84	BE11466	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11466_Mjap_Gib2022_COI	GLTA-079	1	BE11466_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11466	Sample	BE11466	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-079	COI	45	BE11466	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-079	AATTTAATTGTGACTAGGCATGGTCTG	151.18113
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11467_Cmae_Bot2011_COI	GLTA-080	1	BE11467_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	ATGAACAGTCTATCCTCCTTTAG	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11467_Hsan_Wil2021_COI	GLTA-080	1	BE11467_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	CCTGGGCCGGTATAGTAGGT	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11467_Mros_Gib2022_COI	GLTA-080	1	BE11467_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11467_Msal_Bot2012_COI	GLTA-080	1	BE11467_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	GYTAGTTCCRATGATGTTAGCTG	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11467_Mstr_Wil2021_COI	GLTA-080	1	BE11467_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	GTAGTGGTAACTACTCATGCTTTA	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11467_Pvir_Dia2013_COI	GLTA-080	1	BE11467_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	CTTAGTGGCATTAATTCGDAATCC	151.18602
BE11467	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79194	WGS84	BE11467	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11467_Rhar_Sim2018_COI	GLTA-080	1	BE11467_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11467	Sample	BE11467	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-080	COI	45	BE11467	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-080	CTGCTGCTATTGCTCATG	151.18602
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11469_Aamu_Bax2006_COI	GLTA-082	1	BE11469_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	GCACAACCGGGATCTTTACTTC	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11469_Pcan_Bot2011_IGS	GLTA-082	1	BE11469_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-082	IGS	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	CGTAATCCTCAGTACTGGCTA	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11469_Pper_Dia2013_COI	GLTA-082	1	BE11469_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	CTTAGTGGCATTAATTCGDAATCC	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11469_Sspa_Oph2007_COI	GLTA-082	1	BE11469_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	GAAGGCCTTCCGATGATC	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11469_Upin_Bot2011_COI	GLTA-082	1	BE11469_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	CTTTAATTACAGCGTTTTTATTGTTGT	151.18347
BE11469	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79104	WGS84	BE11469	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11469_Vgib_Wil2025_COI	GLTA-082	1	BE11469_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11469	Sample	BE11469	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-082	COI	45	BE11469	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-082	CCCCTCTTTACAAGTTCTTTAGGGT	151.18347
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11470_Cmae_Bot2011_COI	GLTA-083	1	BE11470_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	ATGAACAGTCTATCCTCCTTTAG	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11470_Hsan_Wil2021_COI	GLTA-083	1	BE11470_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	CCTGGGCCGGTATAGTAGGT	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11470_Mros_Gib2022_COI	GLTA-083	1	BE11470_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11470_Msal_Bot2012_COI	GLTA-083	1	BE11470_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	GYTAGTTCCRATGATGTTAGCTG	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11470_Mstr_Wil2021_COI	GLTA-083	1	BE11470_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	GTAGTGGTAACTACTCATGCTTTA	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11470_Pvir_Dia2013_COI	GLTA-083	1	BE11470_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	CTTAGTGGCATTAATTCGDAATCC	151.18476
BE11470	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79078	WGS84	BE11470	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11470_Rhar_Sim2018_COI	GLTA-083	1	BE11470_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11470	Sample	BE11470	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-083	COI	45	BE11470	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-083	CTGCTGCTATTGCTCATG	151.18476
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11472_Aamu_Bax2006_COI	GLTA-085	1	BE11472_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	GCACAACCGGGATCTTTACTTC	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11472_Pcan_Bot2011_IGS	GLTA-085	1	BE11472_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-085	IGS	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	CGTAATCCTCAGTACTGGCTA	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11472_Pper_Dia2013_COI	GLTA-085	1	BE11472_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	CTTAGTGGCATTAATTCGDAATCC	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11472_Sspa_Oph2007_COI	GLTA-085	1	BE11472_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	GAAGGCCTTCCGATGATC	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11472_Upin_Bot2011_COI	GLTA-085	1	BE11472_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	CTTTAATTACAGCGTTTTTATTGTTGT	151.18227
BE11472	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78988	WGS84	BE11472	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11472_Vgib_Wil2025_COI	GLTA-085	1	BE11472_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11472	Sample	BE11472	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-085	COI	45	BE11472	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-085	CCCCTCTTTACAAGTTCTTTAGGGT	151.18227
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11473_Asen_Wil2025_COI	GLTA-086	1	BE11473_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	GTTGTTGTTACAACACATGCTCTTTTG	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11473_Cjap_Sim2018_COI	GLTA-086	1	BE11473_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11473_Esin_And2018_Cyb	GLTA-086	1	BE11473_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-086	Cytb	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	ACCCCTCCTCATATCCAACCA	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11473_Mgig_Bot2012_COI	GLTA-086	1	BE11473_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	TCTTATTCGTTGGAGACTTTATAACCCT	151.18417
BE11473	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78967	WGS84	BE11473	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11473_Mjap_Gib2022_COI	GLTA-086	1	BE11473_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11473	Sample	BE11473	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-086	COI	45	BE11473	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-086	AATTTAATTGTGACTAGGCATGGTCTG	151.18417
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11474_Cmae_Bot2011_COI	GLTA-087	1	BE11474_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	ATGAACAGTCTATCCTCCTTTAG	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11474_Hsan_Wil2021_COI	GLTA-087	1	BE11474_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	CCTGGGCCGGTATAGTAGGT	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11474_Mros_Gib2022_COI	GLTA-087	1	BE11474_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11474_Msal_Bot2012_COI	GLTA-087	1	BE11474_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	GYTAGTTCCRATGATGTTAGCTG	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11474_Mstr_Wil2021_COI	GLTA-087	1	BE11474_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	GTAGTGGTAACTACTCATGCTTTA	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11474_Pvir_Dia2013_COI	GLTA-087	1	BE11474_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	CTTAGTGGCATTAATTCGDAATCC	151.18564
BE11474	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78955	WGS84	BE11474	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11474_Rhar_Sim2018_COI	GLTA-087	1	BE11474_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11474	Sample	BE11474	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-087	COI	45	BE11474	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-087	CTGCTGCTATTGCTCATG	151.18564
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11476_Aamu_Bax2006_COI	GLTA-089	1	BE11476_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	GCACAACCGGGATCTTTACTTC	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11476_Pcan_Bot2011_IGS	GLTA-089	1	BE11476_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-089	IGS	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	CGTAATCCTCAGTACTGGCTA	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11476_Pper_Dia2013_COI	GLTA-089	1	BE11476_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	CTTAGTGGCATTAATTCGDAATCC	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11476_Sspa_Oph2007_COI	GLTA-089	1	BE11476_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	GAAGGCCTTCCGATGATC	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11476_Upin_Bot2011_COI	GLTA-089	1	BE11476_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	CTTTAATTACAGCGTTTTTATTGTTGT	151.18258
BE11476	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7884	WGS84	BE11476	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11476_Vgib_Wil2025_COI	GLTA-089	1	BE11476_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11476	Sample	BE11476	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-089	COI	45	BE11476	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-089	CCCCTCTTTACAAGTTCTTTAGGGT	151.18258
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11477_Asen_Wil2025_COI	GLTA-090	1	BE11477_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	GTTGTTGTTACAACACATGCTCTTTTG	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11477_Cjap_Sim2018_COI	GLTA-090	1	BE11477_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11477_Esin_And2018_Cyb	GLTA-090	1	BE11477_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-090	Cytb	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	ACCCCTCCTCATATCCAACCA	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11477_Mgig_Bot2012_COI	GLTA-090	1	BE11477_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	TCTTATTCGTTGGAGACTTTATAACCCT	151.18425
BE11477	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78833	WGS84	BE11477	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11477_Mjap_Gib2022_COI	GLTA-090	1	BE11477_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11477	Sample	BE11477	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-090	COI	45	BE11477	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-090	AATTTAATTGTGACTAGGCATGGTCTG	151.18425
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11478_Cmae_Bot2011_COI	GLTA-091	1	BE11478_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	ATGAACAGTCTATCCTCCTTTAG	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11478_Hsan_Wil2021_COI	GLTA-091	1	BE11478_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	CCTGGGCCGGTATAGTAGGT	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11478_Mros_Gib2022_COI	GLTA-091	1	BE11478_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11478_Msal_Bot2012_COI	GLTA-091	1	BE11478_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	GYTAGTTCCRATGATGTTAGCTG	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11478_Mstr_Wil2021_COI	GLTA-091	1	BE11478_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	GTAGTGGTAACTACTCATGCTTTA	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11478_Pvir_Dia2013_COI	GLTA-091	1	BE11478_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	CTTAGTGGCATTAATTCGDAATCC	151.1787
BE11478	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.7877	WGS84	BE11478	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11478_Rhar_Sim2018_COI	GLTA-091	1	BE11478_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11478	Sample	BE11478	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-091	COI	45	BE11478	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-091	CTGCTGCTATTGCTCATG	151.1787
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11480_Asen_Wil2025_COI	GLTA-093	1	BE11480_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	GTTGTTGTTACAACACATGCTCTTTTG	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11480_Cjap_Sim2018_COI	GLTA-093	1	BE11480_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11480_Esin_And2018_Cyb	GLTA-093	1	BE11480_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-093	Cytb	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	ACCCCTCCTCATATCCAACCA	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11480_Mgig_Bot2012_COI	GLTA-093	1	BE11480_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	TCTTATTCGTTGGAGACTTTATAACCCT	151.18133
BE11480	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78661	WGS84	BE11480	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11480_Mjap_Gib2022_COI	GLTA-093	1	BE11480_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11480	Sample	BE11480	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-093	COI	45	BE11480	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-093	AATTTAATTGTGACTAGGCATGGTCTG	151.18133
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11481_Cmae_Bot2011_COI	GLTA-094	1	BE11481_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	ATGAACAGTCTATCCTCCTTTAG	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11481_Hsan_Wil2021_COI	GLTA-094	1	BE11481_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	CCTGGGCCGGTATAGTAGGT	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11481_Mros_Gib2022_COI	GLTA-094	1	BE11481_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11481_Msal_Bot2012_COI	GLTA-094	1	BE11481_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	GYTAGTTCCRATGATGTTAGCTG	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11481_Mstr_Wil2021_COI	GLTA-094	1	BE11481_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	GTAGTGGTAACTACTCATGCTTTA	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11481_Pvir_Dia2013_COI	GLTA-094	1	BE11481_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	CTTAGTGGCATTAATTCGDAATCC	151.1775
BE11481	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78664	WGS84	BE11481	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11481_Rhar_Sim2018_COI	GLTA-094	1	BE11481_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11481	Sample	BE11481	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-094	COI	45	BE11481	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-094	CTGCTGCTATTGCTCATG	151.1775
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11483_Aamu_Bax2006_COI	GLTA-096	1	BE11483_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	GCACAACCGGGATCTTTACTTC	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11483_Pcan_Bot2011_IGS	GLTA-096	1	BE11483_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-096	IGS	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	CGTAATCCTCAGTACTGGCTA	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11483_Pper_Dia2013_COI	GLTA-096	1	BE11483_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	CTTAGTGGCATTAATTCGDAATCC	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11483_Sspa_Oph2007_COI	GLTA-096	1	BE11483_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	GAAGGCCTTCCGATGATC	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11483_Upin_Bot2011_COI	GLTA-096	1	BE11483_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	CTTTAATTACAGCGTTTTTATTGTTGT	151.18079
BE11483	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78625	WGS84	BE11483	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11483_Vgib_Wil2025_COI	GLTA-096	1	BE11483_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11483	Sample	BE11483	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-096	COI	45	BE11483	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-096	CCCCTCTTTACAAGTTCTTTAGGGT	151.18079
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11484_Asen_Wil2025_COI	GLTA-097	1	BE11484_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	GTTGTTGTTACAACACATGCTCTTTTG	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11484_Cjap_Sim2018_COI	GLTA-097	1	BE11484_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11484_Esin_And2018_Cyb	GLTA-097	1	BE11484_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-097	Cytb	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	ACCCCTCCTCATATCCAACCA	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11484_Mgig_Bot2012_COI	GLTA-097	1	BE11484_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	TCTTATTCGTTGGAGACTTTATAACCCT	151.17661
BE11484	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78527	WGS84	BE11484	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11484_Mjap_Gib2022_COI	GLTA-097	1	BE11484_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11484	Sample	BE11484	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-097	COI	45	BE11484	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-097	AATTTAATTGTGACTAGGCATGGTCTG	151.17661
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11485_Cmae_Bot2011_COI	GLTA-098	1	BE11485_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	ATGAACAGTCTATCCTCCTTTAG	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11485_Hsan_Wil2021_COI	GLTA-098	1	BE11485_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	CCTGGGCCGGTATAGTAGGT	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11485_Mros_Gib2022_COI	GLTA-098	1	BE11485_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11485_Msal_Bot2012_COI	GLTA-098	1	BE11485_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	GYTAGTTCCRATGATGTTAGCTG	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11485_Mstr_Wil2021_COI	GLTA-098	1	BE11485_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	GTAGTGGTAACTACTCATGCTTTA	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11485_Pvir_Dia2013_COI	GLTA-098	1	BE11485_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	CTTAGTGGCATTAATTCGDAATCC	151.17817
BE11485	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78513	WGS84	BE11485	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11485_Rhar_Sim2018_COI	GLTA-098	1	BE11485_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11485	Sample	BE11485	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-098	COI	45	BE11485	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-098	CTGCTGCTATTGCTCATG	151.17817
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11487_Aamu_Bax2006_COI	GLTA-100	1	BE11487_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	GCACAACCGGGATCTTTACTTC	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11487_Pcan_Bot2011_IGS	GLTA-100	1	BE11487_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-100	IGS	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	CGTAATCCTCAGTACTGGCTA	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11487_Pper_Dia2013_COI	GLTA-100	1	BE11487_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	CTTAGTGGCATTAATTCGDAATCC	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11487_Sspa_Oph2007_COI	GLTA-100	1	BE11487_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	GAAGGCCTTCCGATGATC	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11487_Upin_Bot2011_COI	GLTA-100	1	BE11487_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	CTTTAATTACAGCGTTTTTATTGTTGT	151.17533
BE11487	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78411	WGS84	BE11487	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11487_Vgib_Wil2025_COI	GLTA-100	1	BE11487_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11487	Sample	BE11487	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-100	COI	45	BE11487	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-100	CCCCTCTTTACAAGTTCTTTAGGGT	151.17533
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11488_Asen_Wil2025_COI	GLTA-101	1	BE11488_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	GTTGTTGTTACAACACATGCTCTTTTG	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11488_Cjap_Sim2018_COI	GLTA-101	1	BE11488_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11488_Esin_And2018_Cyb	GLTA-101	1	BE11488_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-101	Cytb	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	ACCCCTCCTCATATCCAACCA	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11488_Mgig_Bot2012_COI	GLTA-101	1	BE11488_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	TCTTATTCGTTGGAGACTTTATAACCCT	151.17702
BE11488	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78399	WGS84	BE11488	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11488_Mjap_Gib2022_COI	GLTA-101	1	BE11488_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11488	Sample	BE11488	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-101	COI	45	BE11488	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-101	AATTTAATTGTGACTAGGCATGGTCTG	151.17702
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11489_Cmae_Bot2011_COI	GLTA-102	1	BE11489_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	ATGAACAGTCTATCCTCCTTTAG	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11489_Hsan_Wil2021_COI	GLTA-102	1	BE11489_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	CCTGGGCCGGTATAGTAGGT	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11489_Mros_Gib2022_COI	GLTA-102	1	BE11489_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11489_Msal_Bot2012_COI	GLTA-102	1	BE11489_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	GYTAGTTCCRATGATGTTAGCTG	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11489_Mstr_Wil2021_COI	GLTA-102	1	BE11489_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	GTAGTGGTAACTACTCATGCTTTA	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11489_Pvir_Dia2013_COI	GLTA-102	1	BE11489_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	CTTAGTGGCATTAATTCGDAATCC	151.17267
BE11489	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78321	WGS84	BE11489	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11489_Rhar_Sim2018_COI	GLTA-102	1	BE11489_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11489	Sample	BE11489	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-102	COI	45	BE11489	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-102	CTGCTGCTATTGCTCATG	151.17267
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11490_Aamu_Bax2006_COI	GLTA-103	1	BE11490_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	GCACAACCGGGATCTTTACTTC	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11490_Pcan_Bot2011_IGS	GLTA-103	1	BE11490_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-103	IGS	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	CGTAATCCTCAGTACTGGCTA	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11490_Pper_Dia2013_COI	GLTA-103	1	BE11490_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	CTTAGTGGCATTAATTCGDAATCC	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11490_Sspa_Oph2007_COI	GLTA-103	1	BE11490_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	GAAGGCCTTCCGATGATC	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11490_Upin_Bot2011_COI	GLTA-103	1	BE11490_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	CTTTAATTACAGCGTTTTTATTGTTGT	151.17436
BE11490	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78305	WGS84	BE11490	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11490_Vgib_Wil2025_COI	GLTA-103	1	BE11490_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11490	Sample	BE11490	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-103	COI	45	BE11490	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-103	CCCCTCTTTACAAGTTCTTTAGGGT	151.17436
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11491_Asen_Wil2025_COI	GLTA-104	1	BE11491_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	GTTGTTGTTACAACACATGCTCTTTTG	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11491_Cjap_Sim2018_COI	GLTA-104	1	BE11491_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11491_Esin_And2018_Cyb	GLTA-104	1	BE11491_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-104	Cytb	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	ACCCCTCCTCATATCCAACCA	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11491_Mgig_Bot2012_COI	GLTA-104	1	BE11491_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	TCTTATTCGTTGGAGACTTTATAACCCT	151.17602
BE11491	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78285	WGS84	BE11491	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11491_Mjap_Gib2022_COI	GLTA-104	1	BE11491_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11491	Sample	BE11491	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-104	COI	45	BE11491	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-104	AATTTAATTGTGACTAGGCATGGTCTG	151.17602
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11492_Cmae_Bot2011_COI	GLTA-105	1	BE11492_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	ATGAACAGTCTATCCTCCTTTAG	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11492_Hsan_Wil2021_COI	GLTA-105	1	BE11492_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	CCTGGGCCGGTATAGTAGGT	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11492_Mros_Gib2022_COI	GLTA-105	1	BE11492_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11492_Msal_Bot2012_COI	GLTA-105	1	BE11492_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	GYTAGTTCCRATGATGTTAGCTG	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11492_Mstr_Wil2021_COI	GLTA-105	1	BE11492_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	GTAGTGGTAACTACTCATGCTTTA	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11492_Pvir_Dia2013_COI	GLTA-105	1	BE11492_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	CTTAGTGGCATTAATTCGDAATCC	151.17294
BE11492	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.78179	WGS84	BE11492	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11492_Rhar_Sim2018_COI	GLTA-105	1	BE11492_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11492	Sample	BE11492	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-105	COI	45	BE11492	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-105	CTGCTGCTATTGCTCATG	151.17294
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11498_Aamu_Bax2006_COI	GLTA-072	1	BE11498_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	GCACAACCGGGATCTTTACTTC	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11498_Pcan_Bot2011_IGS	GLTA-072	1	BE11498_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTA-072	IGS	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	CGTAATCCTCAGTACTGGCTA	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11498_Pper_Dia2013_COI	GLTA-072	1	BE11498_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Pper_Dia2013_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	CTTAGTGGCATTAATTCGDAATCC	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11498_Sspa_Oph2007_COI	GLTA-072	1	BE11498_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	GAAGGCCTTCCGATGATC	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11498_Upin_Bot2011_COI	GLTA-072	1	BE11498_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Upin_Bot2011_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	CTTTAATTACAGCGTTTTTATTGTTGT	151.18449
BE11498	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79496	WGS84	BE11498	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11498_Vgib_Wil2025_COI	GLTA-072	1	BE11498_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11498	Sample	BE11498	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTA-072	COI	45	BE11498	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-072	CCCCTCTTTACAAGTTCTTTAGGGT	151.18449
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11499_Asen_Wil2025_COI	GLTA-073	1	BE11499_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Asen_Wil2025_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	GTTGTTGTTACAACACATGCTCTTTTG	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11499_Cjap_Sim2018_COI	GLTA-073	1	BE11499_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11499_Esin_And2018_Cyb	GLTA-073	1	BE11499_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Esin_And2018_Cyb	GLTA-073	Cytb	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	ACCCCTCCTCATATCCAACCA	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11499_Mgig_Bot2012_COI	GLTA-073	1	BE11499_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	TCTTATTCGTTGGAGACTTTATAACCCT	151.18617
BE11499	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79461	WGS84	BE11499	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11499_Mjap_Gib2022_COI	GLTA-073	1	BE11499_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11499	Sample	BE11499	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTA-073	COI	45	BE11499	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-073	AATTTAATTGTGACTAGGCATGGTCTG	151.18617
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11500_Cmae_Bot2011_COI	GLTA-074	1	BE11500_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	ATGAACAGTCTATCCTCCTTTAG	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11500_Hsan_Wil2021_COI	GLTA-074	1	BE11500_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	CCTGGGCCGGTATAGTAGGT	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11500_Mros_Gib2022_COI	GLTA-074	1	BE11500_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Mros_Gib2022_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11500_Msal_Bot2012_COI	GLTA-074	1	BE11500_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Msal_Bot2012_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	GYTAGTTCCRATGATGTTAGCTG	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11500_Mstr_Wil2021_COI	GLTA-074	1	BE11500_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	GTAGTGGTAACTACTCATGCTTTA	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11500_Pvir_Dia2013_COI	GLTA-074	1	BE11500_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	CTTAGTGGCATTAATTCGDAATCC	151.18815
BE11500	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16041	-23.79434	WGS84	BE11500	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_A	25/10/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11500_Rhar_Sim2018_COI	GLTA-074	1	BE11500_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11500	Sample	BE11500	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTA-074	COI	45	BE11500	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTA-074	CTGCTGCTATTGCTCATG	151.18815
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11502_Aamu_Bax2006_COI	DRWA-001	1	BE11502_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	GCACAACCGGGATCTTTACTTC	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11502_Pcan_Bot2011_IGS	DRWA-001	1	BE11502_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-001	IGS	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	CGTAATCCTCAGTACTGGCTA	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11502_Pper_Dia2013_COI	DRWA-001	1	BE11502_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	CTTAGTGGCATTAATTCGDAATCC	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11502_Sspa_Oph2007_COI	DRWA-001	1	BE11502_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	GAAGGCCTTCCGATGATC	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11502_Upin_Bot2011_COI	DRWA-001	1	BE11502_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	CTTTAATTACAGCGTTTTTATTGTTGT	130.84747
BE11502	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52847	WGS84	BE11502	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11502_Vgib_Wil2025_COI	DRWA-001	1	BE11502_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11502	Sample	BE11502	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-001	COI	45	BE11502	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-001	CCCCTCTTTACAAGTTCTTTAGGGT	130.84747
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11503_Asen_Wil2025_COI	DRWA-002	1	BE11503_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	GTTGTTGTTACAACACATGCTCTTTTG	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11503_Cjap_Sim2018_COI	DRWA-002	1	BE11503_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11503_Esin_And2018_Cyb	DRWA-002	1	BE11503_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-002	Cytb	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	ACCCCTCCTCATATCCAACCA	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11503_Mgig_Bot2012_COI	DRWA-002	1	BE11503_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	TCTTATTCGTTGGAGACTTTATAACCCT	130.84915
BE11503	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52846	WGS84	BE11503	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11503_Mjap_Gib2022_COI	DRWA-002	1	BE11503_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11503	Sample	BE11503	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-002	COI	45	BE11503	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-002	AATTTAATTGTGACTAGGCATGGTCTG	130.84915
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11504_Cmae_Bot2011_COI	DRWA-003	1	BE11504_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	ATGAACAGTCTATCCTCCTTTAG	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11504_Hsan_Wil2021_COI	DRWA-003	1	BE11504_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	CCTGGGCCGGTATAGTAGGT	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11504_Mros_Gib2022_COI	DRWA-003	1	BE11504_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11504_Msal_Bot2012_COI	DRWA-003	1	BE11504_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	GYTAGTTCCRATGATGTTAGCTG	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11504_Mstr_Wil2021_COI	DRWA-003	1	BE11504_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	GTAGTGGTAACTACTCATGCTTTA	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11504_Pvir_Dia2013_COI	DRWA-003	1	BE11504_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	CTTAGTGGCATTAATTCGDAATCC	130.85052
BE11504	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52818	WGS84	BE11504	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11504_Rhar_Sim2018_COI	DRWA-003	1	BE11504_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11504	Sample	BE11504	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-003	COI	45	BE11504	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-003	CTGCTGCTATTGCTCATG	130.85052
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11506_Aamu_Bax2006_COI	DRWA-005	1	BE11506_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	GCACAACCGGGATCTTTACTTC	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11506_Pcan_Bot2011_IGS	DRWA-005	1	BE11506_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-005	IGS	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	CGTAATCCTCAGTACTGGCTA	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11506_Pper_Dia2013_COI	DRWA-005	1	BE11506_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	CTTAGTGGCATTAATTCGDAATCC	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11506_Sspa_Oph2007_COI	DRWA-005	1	BE11506_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	GAAGGCCTTCCGATGATC	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11506_Upin_Bot2011_COI	DRWA-005	1	BE11506_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	CTTTAATTACAGCGTTTTTATTGTTGT	130.85324
BE11506	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52827	WGS84	BE11506	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11506_Vgib_Wil2025_COI	DRWA-005	1	BE11506_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11506	Sample	BE11506	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-005	COI	45	BE11506	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-005	CCCCTCTTTACAAGTTCTTTAGGGT	130.85324
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11507_Asen_Wil2025_COI	DRWA-006	1	BE11507_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	GTTGTTGTTACAACACATGCTCTTTTG	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11507_Cjap_Sim2018_COI	DRWA-006	1	BE11507_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11507_Esin_And2018_Cyb	DRWA-006	1	BE11507_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-006	Cytb	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	ACCCCTCCTCATATCCAACCA	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11507_Mgig_Bot2012_COI	DRWA-006	1	BE11507_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	TCTTATTCGTTGGAGACTTTATAACCCT	130.84666
BE11507	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52691	WGS84	BE11507	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11507_Mjap_Gib2022_COI	DRWA-006	1	BE11507_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11507	Sample	BE11507	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-006	COI	45	BE11507	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-006	AATTTAATTGTGACTAGGCATGGTCTG	130.84666
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11508_Cmae_Bot2011_COI	DRWA-007	1	BE11508_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	ATGAACAGTCTATCCTCCTTTAG	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11508_Hsan_Wil2021_COI	DRWA-007	1	BE11508_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	CCTGGGCCGGTATAGTAGGT	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11508_Mros_Gib2022_COI	DRWA-007	1	BE11508_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11508_Msal_Bot2012_COI	DRWA-007	1	BE11508_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	GYTAGTTCCRATGATGTTAGCTG	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11508_Mstr_Wil2021_COI	DRWA-007	1	BE11508_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	GTAGTGGTAACTACTCATGCTTTA	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11508_Pvir_Dia2013_COI	DRWA-007	1	BE11508_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	CTTAGTGGCATTAATTCGDAATCC	130.84857
BE11508	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52733	WGS84	BE11508	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11508_Rhar_Sim2018_COI	DRWA-007	1	BE11508_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11508	Sample	BE11508	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-007	COI	45	BE11508	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-007	CTGCTGCTATTGCTCATG	130.84857
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11510_Asen_Wil2025_COI	DRWA-009	1	BE11510_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	GTTGTTGTTACAACACATGCTCTTTTG	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11510_Cjap_Sim2018_COI	DRWA-009	1	BE11510_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11510_Esin_And2018_Cyb	DRWA-009	1	BE11510_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-009	Cytb	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	ACCCCTCCTCATATCCAACCA	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11510_Mgig_Bot2012_COI	DRWA-009	1	BE11510_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	TCTTATTCGTTGGAGACTTTATAACCCT	130.85118
BE11510	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52708	WGS84	BE11510	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11510_Mjap_Gib2022_COI	DRWA-009	1	BE11510_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11510	Sample	BE11510	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-009	COI	45	BE11510	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-009	AATTTAATTGTGACTAGGCATGGTCTG	130.85118
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11511_Cmae_Bot2011_COI	DRWA-010	1	BE11511_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	ATGAACAGTCTATCCTCCTTTAG	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11511_Hsan_Wil2021_COI	DRWA-010	1	BE11511_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	CCTGGGCCGGTATAGTAGGT	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11511_Mros_Gib2022_COI	DRWA-010	1	BE11511_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11511_Msal_Bot2012_COI	DRWA-010	1	BE11511_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	GYTAGTTCCRATGATGTTAGCTG	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11511_Mstr_Wil2021_COI	DRWA-010	1	BE11511_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	GTAGTGGTAACTACTCATGCTTTA	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11511_Pvir_Dia2013_COI	DRWA-010	1	BE11511_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	CTTAGTGGCATTAATTCGDAATCC	130.85253
BE11511	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52709	WGS84	BE11511	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11511_Rhar_Sim2018_COI	DRWA-010	1	BE11511_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11511	Sample	BE11511	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-010	COI	45	BE11511	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-010	CTGCTGCTATTGCTCATG	130.85253
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11513_Aamu_Bax2006_COI	DRWA-012	1	BE11513_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	GCACAACCGGGATCTTTACTTC	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11513_Pcan_Bot2011_IGS	DRWA-012	1	BE11513_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-012	IGS	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	CGTAATCCTCAGTACTGGCTA	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11513_Pper_Dia2013_COI	DRWA-012	1	BE11513_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	CTTAGTGGCATTAATTCGDAATCC	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11513_Sspa_Oph2007_COI	DRWA-012	1	BE11513_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	GAAGGCCTTCCGATGATC	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11513_Upin_Bot2011_COI	DRWA-012	1	BE11513_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	CTTTAATTACAGCGTTTTTATTGTTGT	130.84598
BE11513	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5257	WGS84	BE11513	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11513_Vgib_Wil2025_COI	DRWA-012	1	BE11513_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11513	Sample	BE11513	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-012	COI	45	BE11513	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-012	CCCCTCTTTACAAGTTCTTTAGGGT	130.84598
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11514_Asen_Wil2025_COI	DRWA-013	1	BE11514_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	GTTGTTGTTACAACACATGCTCTTTTG	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11514_Cjap_Sim2018_COI	DRWA-013	1	BE11514_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11514_Esin_And2018_Cyb	DRWA-013	1	BE11514_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-013	Cytb	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	ACCCCTCCTCATATCCAACCA	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11514_Mgig_Bot2012_COI	DRWA-013	1	BE11514_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	TCTTATTCGTTGGAGACTTTATAACCCT	130.84737
BE11514	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52567	WGS84	BE11514	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11514_Mjap_Gib2022_COI	DRWA-013	1	BE11514_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11514	Sample	BE11514	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-013	COI	45	BE11514	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-013	AATTTAATTGTGACTAGGCATGGTCTG	130.84737
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11515_Cmae_Bot2011_COI	DRWA-014	1	BE11515_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	ATGAACAGTCTATCCTCCTTTAG	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11515_Hsan_Wil2021_COI	DRWA-014	1	BE11515_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	CCTGGGCCGGTATAGTAGGT	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11515_Mros_Gib2022_COI	DRWA-014	1	BE11515_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11515_Msal_Bot2012_COI	DRWA-014	1	BE11515_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	GYTAGTTCCRATGATGTTAGCTG	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11515_Mstr_Wil2021_COI	DRWA-014	1	BE11515_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	GTAGTGGTAACTACTCATGCTTTA	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11515_Pvir_Dia2013_COI	DRWA-014	1	BE11515_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	CTTAGTGGCATTAATTCGDAATCC	130.84539
BE11515	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52459	WGS84	BE11515	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11515_Rhar_Sim2018_COI	DRWA-014	1	BE11515_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11515	Sample	BE11515	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-014	COI	45	BE11515	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-014	CTGCTGCTATTGCTCATG	130.84539
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11517_Aamu_Bax2006_COI	DRWA-016	1	BE11517_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	GCACAACCGGGATCTTTACTTC	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11517_Pcan_Bot2011_IGS	DRWA-016	1	BE11517_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-016	IGS	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	CGTAATCCTCAGTACTGGCTA	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11517_Pper_Dia2013_COI	DRWA-016	1	BE11517_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	CTTAGTGGCATTAATTCGDAATCC	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11517_Sspa_Oph2007_COI	DRWA-016	1	BE11517_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	GAAGGCCTTCCGATGATC	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11517_Upin_Bot2011_COI	DRWA-016	1	BE11517_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	CTTTAATTACAGCGTTTTTATTGTTGT	130.84482
BE11517	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52358	WGS84	BE11517	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11517_Vgib_Wil2025_COI	DRWA-016	1	BE11517_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11517	Sample	BE11517	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-016	COI	45	BE11517	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-016	CCCCTCTTTACAAGTTCTTTAGGGT	130.84482
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11518_Asen_Wil2025_COI	DRWA-017	1	BE11518_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	GTTGTTGTTACAACACATGCTCTTTTG	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11518_Cjap_Sim2018_COI	DRWA-017	1	BE11518_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11518_Esin_And2018_Cyb	DRWA-017	1	BE11518_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-017	Cytb	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	ACCCCTCCTCATATCCAACCA	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11518_Mgig_Bot2012_COI	DRWA-017	1	BE11518_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	TCTTATTCGTTGGAGACTTTATAACCCT	130.846
BE11518	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52355	WGS84	BE11518	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11518_Mjap_Gib2022_COI	DRWA-017	1	BE11518_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11518	Sample	BE11518	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-017	COI	45	BE11518	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-017	AATTTAATTGTGACTAGGCATGGTCTG	130.846
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11519_Cmae_Bot2011_COI	DRWA-018	1	BE11519_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	ATGAACAGTCTATCCTCCTTTAG	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11519_Hsan_Wil2021_COI	DRWA-018	1	BE11519_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	CCTGGGCCGGTATAGTAGGT	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11519_Mros_Gib2022_COI	DRWA-018	1	BE11519_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11519_Msal_Bot2012_COI	DRWA-018	1	BE11519_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	GYTAGTTCCRATGATGTTAGCTG	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11519_Mstr_Wil2021_COI	DRWA-018	1	BE11519_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	GTAGTGGTAACTACTCATGCTTTA	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11519_Pvir_Dia2013_COI	DRWA-018	1	BE11519_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	CTTAGTGGCATTAATTCGDAATCC	130.84534
BE11519	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52225	WGS84	BE11519	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11519_Rhar_Sim2018_COI	DRWA-018	1	BE11519_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11519	Sample	BE11519	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-018	COI	45	BE11519	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-018	CTGCTGCTATTGCTCATG	130.84534
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11520_Aamu_Bax2006_COI	DRWA-019	1	BE11520_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	GCACAACCGGGATCTTTACTTC	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11520_Pcan_Bot2011_IGS	DRWA-019	1	BE11520_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-019	IGS	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	CGTAATCCTCAGTACTGGCTA	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11520_Pper_Dia2013_COI	DRWA-019	1	BE11520_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	CTTAGTGGCATTAATTCGDAATCC	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11520_Sspa_Oph2007_COI	DRWA-019	1	BE11520_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	GAAGGCCTTCCGATGATC	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11520_Upin_Bot2011_COI	DRWA-019	1	BE11520_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	CTTTAATTACAGCGTTTTTATTGTTGT	130.84673
BE11520	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52236	WGS84	BE11520	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11520_Vgib_Wil2025_COI	DRWA-019	1	BE11520_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11520	Sample	BE11520	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-019	COI	45	BE11520	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-019	CCCCTCTTTACAAGTTCTTTAGGGT	130.84673
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11521_Asen_Wil2025_COI	DRWA-020	1	BE11521_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	GTTGTTGTTACAACACATGCTCTTTTG	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11521_Cjap_Sim2018_COI	DRWA-020	1	BE11521_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11521_Esin_And2018_Cyb	DRWA-020	1	BE11521_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-020	Cytb	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	ACCCCTCCTCATATCCAACCA	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11521_Mgig_Bot2012_COI	DRWA-020	1	BE11521_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	TCTTATTCGTTGGAGACTTTATAACCCT	130.84479
BE11521	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52113	WGS84	BE11521	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11521_Mjap_Gib2022_COI	DRWA-020	1	BE11521_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11521	Sample	BE11521	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-020	COI	45	BE11521	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-020	AATTTAATTGTGACTAGGCATGGTCTG	130.84479
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11522_Cmae_Bot2011_COI	DRWA-021	1	BE11522_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	ATGAACAGTCTATCCTCCTTTAG	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11522_Hsan_Wil2021_COI	DRWA-021	1	BE11522_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	CCTGGGCCGGTATAGTAGGT	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11522_Mros_Gib2022_COI	DRWA-021	1	BE11522_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11522_Msal_Bot2012_COI	DRWA-021	1	BE11522_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	GYTAGTTCCRATGATGTTAGCTG	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11522_Mstr_Wil2021_COI	DRWA-021	1	BE11522_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	GTAGTGGTAACTACTCATGCTTTA	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11522_Pvir_Dia2013_COI	DRWA-021	1	BE11522_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	CTTAGTGGCATTAATTCGDAATCC	130.84612
BE11522	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52122	WGS84	BE11522	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11522_Rhar_Sim2018_COI	DRWA-021	1	BE11522_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11522	Sample	BE11522	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-021	COI	45	BE11522	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-021	CTGCTGCTATTGCTCATG	130.84612
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11524_Aamu_Bax2006_COI	DRWA-023	1	BE11524_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	GCACAACCGGGATCTTTACTTC	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11524_Pcan_Bot2011_IGS	DRWA-023	1	BE11524_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-023	IGS	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	CGTAATCCTCAGTACTGGCTA	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11524_Pper_Dia2013_COI	DRWA-023	1	BE11524_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	CTTAGTGGCATTAATTCGDAATCC	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11524_Sspa_Oph2007_COI	DRWA-023	1	BE11524_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	GAAGGCCTTCCGATGATC	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11524_Upin_Bot2011_COI	DRWA-023	1	BE11524_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	CTTTAATTACAGCGTTTTTATTGTTGT	130.84894
BE11524	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52056	WGS84	BE11524	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11524_Vgib_Wil2025_COI	DRWA-023	1	BE11524_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11524	Sample	BE11524	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-023	COI	45	BE11524	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-023	CCCCTCTTTACAAGTTCTTTAGGGT	130.84894
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11525_Asen_Wil2025_COI	DRWA-024	1	BE11525_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	GTTGTTGTTACAACACATGCTCTTTTG	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11525_Cjap_Sim2018_COI	DRWA-024	1	BE11525_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11525_Esin_And2018_Cyb	DRWA-024	1	BE11525_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-024	Cytb	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	ACCCCTCCTCATATCCAACCA	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11525_Mgig_Bot2012_COI	DRWA-024	1	BE11525_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	TCTTATTCGTTGGAGACTTTATAACCCT	130.85033
BE11525	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52066	WGS84	BE11525	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11525_Mjap_Gib2022_COI	DRWA-024	1	BE11525_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11525	Sample	BE11525	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-024	COI	45	BE11525	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-024	AATTTAATTGTGACTAGGCATGGTCTG	130.85033
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11526_Cmae_Bot2011_COI	DRWA-025	1	BE11526_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	ATGAACAGTCTATCCTCCTTTAG	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11526_Hsan_Wil2021_COI	DRWA-025	1	BE11526_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	CCTGGGCCGGTATAGTAGGT	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11526_Mros_Gib2022_COI	DRWA-025	1	BE11526_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11526_Msal_Bot2012_COI	DRWA-025	1	BE11526_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	GYTAGTTCCRATGATGTTAGCTG	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11526_Mstr_Wil2021_COI	DRWA-025	1	BE11526_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	GTAGTGGTAACTACTCATGCTTTA	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11526_Pvir_Dia2013_COI	DRWA-025	1	BE11526_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	CTTAGTGGCATTAATTCGDAATCC	130.84542
BE11526	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11526	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11526_Rhar_Sim2018_COI	DRWA-025	1	BE11526_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11526	Sample	BE11526	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-025	COI	45	BE11526	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-025	CTGCTGCTATTGCTCATG	130.84542
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11528_Aamu_Bax2006_COI	DRWA-027	1	BE11528_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	GCACAACCGGGATCTTTACTTC	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11528_Pcan_Bot2011_IGS	DRWA-027	1	BE11528_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-027	IGS	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	CGTAATCCTCAGTACTGGCTA	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11528_Pper_Dia2013_COI	DRWA-027	1	BE11528_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	CTTAGTGGCATTAATTCGDAATCC	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11528_Sspa_Oph2007_COI	DRWA-027	1	BE11528_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	GAAGGCCTTCCGATGATC	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11528_Upin_Bot2011_COI	DRWA-027	1	BE11528_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	CTTTAATTACAGCGTTTTTATTGTTGT	130.848
BE11528	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5201	WGS84	BE11528	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11528_Vgib_Wil2025_COI	DRWA-027	1	BE11528_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11528	Sample	BE11528	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-027	COI	45	BE11528	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-027	CCCCTCTTTACAAGTTCTTTAGGGT	130.848
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11529_Asen_Wil2025_COI	DRWA-028	1	BE11529_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	GTTGTTGTTACAACACATGCTCTTTTG	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11529_Cjap_Sim2018_COI	DRWA-028	1	BE11529_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11529_Esin_And2018_Cyb	DRWA-028	1	BE11529_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-028	Cytb	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	ACCCCTCCTCATATCCAACCA	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11529_Mgig_Bot2012_COI	DRWA-028	1	BE11529_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	TCTTATTCGTTGGAGACTTTATAACCCT	130.84944
BE11529	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52005	WGS84	BE11529	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11529_Mjap_Gib2022_COI	DRWA-028	1	BE11529_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11529	Sample	BE11529	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-028	COI	45	BE11529	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-028	AATTTAATTGTGACTAGGCATGGTCTG	130.84944
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11531_Aamu_Bax2006_COI	DRWA-030	1	BE11531_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	GCACAACCGGGATCTTTACTTC	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11531_Pcan_Bot2011_IGS	DRWA-030	1	BE11531_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-030	IGS	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	CGTAATCCTCAGTACTGGCTA	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11531_Pper_Dia2013_COI	DRWA-030	1	BE11531_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	CTTAGTGGCATTAATTCGDAATCC	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11531_Sspa_Oph2007_COI	DRWA-030	1	BE11531_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	GAAGGCCTTCCGATGATC	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11531_Upin_Bot2011_COI	DRWA-030	1	BE11531_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	CTTTAATTACAGCGTTTTTATTGTTGT	130.84867
BE11531	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51892	WGS84	BE11531	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11531_Vgib_Wil2025_COI	DRWA-030	1	BE11531_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11531	Sample	BE11531	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-030	COI	45	BE11531	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-030	CCCCTCTTTACAAGTTCTTTAGGGT	130.84867
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11532_Asen_Wil2025_COI	DRWA-031	1	BE11532_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	GTTGTTGTTACAACACATGCTCTTTTG	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11532_Cjap_Sim2018_COI	DRWA-031	1	BE11532_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11532_Esin_And2018_Cyb	DRWA-031	1	BE11532_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-031	Cytb	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	ACCCCTCCTCATATCCAACCA	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11532_Mgig_Bot2012_COI	DRWA-031	1	BE11532_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	TCTTATTCGTTGGAGACTTTATAACCCT	130.85026
BE11532	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51907	WGS84	BE11532	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11532_Mjap_Gib2022_COI	DRWA-031	1	BE11532_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11532	Sample	BE11532	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-031	COI	45	BE11532	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-031	AATTTAATTGTGACTAGGCATGGTCTG	130.85026
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11533_Cmae_Bot2011_COI	DRWA-032	1	BE11533_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	ATGAACAGTCTATCCTCCTTTAG	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11533_Hsan_Wil2021_COI	DRWA-032	1	BE11533_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	CCTGGGCCGGTATAGTAGGT	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11533_Mros_Gib2022_COI	DRWA-032	1	BE11533_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11533_Msal_Bot2012_COI	DRWA-032	1	BE11533_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	GYTAGTTCCRATGATGTTAGCTG	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11533_Mstr_Wil2021_COI	DRWA-032	1	BE11533_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	GTAGTGGTAACTACTCATGCTTTA	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11533_Pvir_Dia2013_COI	DRWA-032	1	BE11533_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	CTTAGTGGCATTAATTCGDAATCC	130.85127
BE11533	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5217	WGS84	BE11533	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11533_Rhar_Sim2018_COI	DRWA-032	1	BE11533_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11533	Sample	BE11533	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-032	COI	45	BE11533	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-032	CTGCTGCTATTGCTCATG	130.85127
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11535_Aamu_Bax2006_COI	DRWA-034	1	BE11535_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	GCACAACCGGGATCTTTACTTC	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11535_Pcan_Bot2011_IGS	DRWA-034	1	BE11535_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-034	IGS	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	CGTAATCCTCAGTACTGGCTA	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11535_Pper_Dia2013_COI	DRWA-034	1	BE11535_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	CTTAGTGGCATTAATTCGDAATCC	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11535_Sspa_Oph2007_COI	DRWA-034	1	BE11535_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	GAAGGCCTTCCGATGATC	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11535_Upin_Bot2011_COI	DRWA-034	1	BE11535_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	CTTTAATTACAGCGTTTTTATTGTTGT	130.84775
BE11535	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52466	WGS84	BE11535	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11535_Vgib_Wil2025_COI	DRWA-034	1	BE11535_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11535	Sample	BE11535	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-034	COI	45	BE11535	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-034	CCCCTCTTTACAAGTTCTTTAGGGT	130.84775
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11536_Asen_Wil2025_COI	DRWA-035	1	BE11536_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	GTTGTTGTTACAACACATGCTCTTTTG	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11536_Cjap_Sim2018_COI	DRWA-035	1	BE11536_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11536_Esin_And2018_Cyb	DRWA-035	1	BE11536_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-035	Cytb	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	ACCCCTCCTCATATCCAACCA	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11536_Mgig_Bot2012_COI	DRWA-035	1	BE11536_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	TCTTATTCGTTGGAGACTTTATAACCCT	130.85165
BE11536	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.52492	WGS84	BE11536	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_A	11/06/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11536_Mjap_Gib2022_COI	DRWA-035	1	BE11536_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11536	Sample	BE11536	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-035	COI	45	BE11536	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-035	AATTTAATTGTGACTAGGCATGGTCTG	130.85165
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11542_Aamu_Bax2006_COI	DRWA-107	1	BE11542_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	GCACAACCGGGATCTTTACTTC	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11542_Pcan_Bot2011_IGS	DRWA-107	1	BE11542_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-107	IGS	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	CGTAATCCTCAGTACTGGCTA	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11542_Pper_Dia2013_COI	DRWA-107	1	BE11542_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	CTTAGTGGCATTAATTCGDAATCC	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11542_Sspa_Oph2007_COI	DRWA-107	1	BE11542_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	GAAGGCCTTCCGATGATC	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11542_Upin_Bot2011_COI	DRWA-107	1	BE11542_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	CTTTAATTACAGCGTTTTTATTGTTGT	130.9044
BE11542	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51245	WGS84	BE11542	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11542_Vgib_Wil2025_COI	DRWA-107	1	BE11542_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11542	Sample	BE11542	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-107	COI	45	BE11542	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-107	CCCCTCTTTACAAGTTCTTTAGGGT	130.9044
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11543_Asen_Wil2025_COI	DRWA-108	1	BE11543_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	GTTGTTGTTACAACACATGCTCTTTTG	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11543_Cjap_Sim2018_COI	DRWA-108	1	BE11543_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11543_Esin_And2018_Cyb	DRWA-108	1	BE11543_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-108	Cytb	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	ACCCCTCCTCATATCCAACCA	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11543_Mgig_Bot2012_COI	DRWA-108	1	BE11543_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	TCTTATTCGTTGGAGACTTTATAACCCT	130.90387
BE11543	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.51139	WGS84	BE11543	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11543_Mjap_Gib2022_COI	DRWA-108	1	BE11543_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11543	Sample	BE11543	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-108	COI	45	BE11543	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-108	AATTTAATTGTGACTAGGCATGGTCTG	130.90387
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11544_Cmae_Bot2011_COI	DRWA-109	1	BE11544_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	ATGAACAGTCTATCCTCCTTTAG	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11544_Hsan_Wil2021_COI	DRWA-109	1	BE11544_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	CCTGGGCCGGTATAGTAGGT	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11544_Mros_Gib2022_COI	DRWA-109	1	BE11544_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11544_Msal_Bot2012_COI	DRWA-109	1	BE11544_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	GYTAGTTCCRATGATGTTAGCTG	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11544_Mstr_Wil2021_COI	DRWA-109	1	BE11544_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	GTAGTGGTAACTACTCATGCTTTA	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11544_Pvir_Dia2013_COI	DRWA-109	1	BE11544_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	CTTAGTGGCATTAATTCGDAATCC	130.90443
BE11544	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5104	WGS84	BE11544	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11544_Rhar_Sim2018_COI	DRWA-109	1	BE11544_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11544	Sample	BE11544	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-109	COI	45	BE11544	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-109	CTGCTGCTATTGCTCATG	130.90443
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11546_Aamu_Bax2006_COI	DRWA-111	1	BE11546_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	GCACAACCGGGATCTTTACTTC	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11546_Pcan_Bot2011_IGS	DRWA-111	1	BE11546_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-111	IGS	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	CGTAATCCTCAGTACTGGCTA	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11546_Pper_Dia2013_COI	DRWA-111	1	BE11546_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	CTTAGTGGCATTAATTCGDAATCC	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11546_Sspa_Oph2007_COI	DRWA-111	1	BE11546_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	GAAGGCCTTCCGATGATC	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11546_Upin_Bot2011_COI	DRWA-111	1	BE11546_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	CTTTAATTACAGCGTTTTTATTGTTGT	130.90358
BE11546	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5096	WGS84	BE11546	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11546_Vgib_Wil2025_COI	DRWA-111	1	BE11546_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11546	Sample	BE11546	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-111	COI	45	BE11546	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-111	CCCCTCTTTACAAGTTCTTTAGGGT	130.90358
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11547_Asen_Wil2025_COI	DRWA-112	1	BE11547_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	GTTGTTGTTACAACACATGCTCTTTTG	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11547_Cjap_Sim2018_COI	DRWA-112	1	BE11547_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11547_Esin_And2018_Cyb	DRWA-112	1	BE11547_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-112	Cytb	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	ACCCCTCCTCATATCCAACCA	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11547_Mgig_Bot2012_COI	DRWA-112	1	BE11547_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	TCTTATTCGTTGGAGACTTTATAACCCT	130.90503
BE11547	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50939	WGS84	BE11547	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11547_Mjap_Gib2022_COI	DRWA-112	1	BE11547_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11547	Sample	BE11547	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-112	COI	45	BE11547	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-112	AATTTAATTGTGACTAGGCATGGTCTG	130.90503
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11548_Cmae_Bot2011_COI	DRWA-113	1	BE11548_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	ATGAACAGTCTATCCTCCTTTAG	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11548_Hsan_Wil2021_COI	DRWA-113	1	BE11548_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	CCTGGGCCGGTATAGTAGGT	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11548_Mros_Gib2022_COI	DRWA-113	1	BE11548_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11548_Msal_Bot2012_COI	DRWA-113	1	BE11548_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	GYTAGTTCCRATGATGTTAGCTG	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11548_Mstr_Wil2021_COI	DRWA-113	1	BE11548_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	GTAGTGGTAACTACTCATGCTTTA	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11548_Pvir_Dia2013_COI	DRWA-113	1	BE11548_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	CTTAGTGGCATTAATTCGDAATCC	130.90438
BE11548	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50837	WGS84	BE11548	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11548_Rhar_Sim2018_COI	DRWA-113	1	BE11548_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11548	Sample	BE11548	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-113	COI	45	BE11548	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-113	CTGCTGCTATTGCTCATG	130.90438
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11550_Asen_Wil2025_COI	DRWA-115	1	BE11550_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	GTTGTTGTTACAACACATGCTCTTTTG	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11550_Cjap_Sim2018_COI	DRWA-115	1	BE11550_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11550_Esin_And2018_Cyb	DRWA-115	1	BE11550_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-115	Cytb	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	ACCCCTCCTCATATCCAACCA	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11550_Mgig_Bot2012_COI	DRWA-115	1	BE11550_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	TCTTATTCGTTGGAGACTTTATAACCCT	130.90702
BE11550	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50835	WGS84	BE11550	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11550_Mjap_Gib2022_COI	DRWA-115	1	BE11550_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11550	Sample	BE11550	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-115	COI	45	BE11550	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-115	AATTTAATTGTGACTAGGCATGGTCTG	130.90702
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11551_Cmae_Bot2011_COI	DRWA-116	1	BE11551_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	ATGAACAGTCTATCCTCCTTTAG	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11551_Hsan_Wil2021_COI	DRWA-116	1	BE11551_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	CCTGGGCCGGTATAGTAGGT	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11551_Mros_Gib2022_COI	DRWA-116	1	BE11551_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11551_Msal_Bot2012_COI	DRWA-116	1	BE11551_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	GYTAGTTCCRATGATGTTAGCTG	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11551_Mstr_Wil2021_COI	DRWA-116	1	BE11551_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	GTAGTGGTAACTACTCATGCTTTA	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11551_Pvir_Dia2013_COI	DRWA-116	1	BE11551_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	CTTAGTGGCATTAATTCGDAATCC	130.90822
BE11551	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50843	WGS84	BE11551	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11551_Rhar_Sim2018_COI	DRWA-116	1	BE11551_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11551	Sample	BE11551	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-116	COI	45	BE11551	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-116	CTGCTGCTATTGCTCATG	130.90822
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11553_Aamu_Bax2006_COI	DRWA-118	1	BE11553_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	GCACAACCGGGATCTTTACTTC	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11553_Pcan_Bot2011_IGS	DRWA-118	1	BE11553_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-118	IGS	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	CGTAATCCTCAGTACTGGCTA	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11553_Pper_Dia2013_COI	DRWA-118	1	BE11553_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	CTTAGTGGCATTAATTCGDAATCC	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11553_Sspa_Oph2007_COI	DRWA-118	1	BE11553_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	GAAGGCCTTCCGATGATC	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11553_Upin_Bot2011_COI	DRWA-118	1	BE11553_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	CTTTAATTACAGCGTTTTTATTGTTGT	130.90494
BE11553	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11553	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11553_Vgib_Wil2025_COI	DRWA-118	1	BE11553_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11553	Sample	BE11553	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-118	COI	45	BE11553	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-118	CCCCTCTTTACAAGTTCTTTAGGGT	130.90494
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11554_Asen_Wil2025_COI	DRWA-119	1	BE11554_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	GTTGTTGTTACAACACATGCTCTTTTG	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11554_Cjap_Sim2018_COI	DRWA-119	1	BE11554_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11554_Esin_And2018_Cyb	DRWA-119	1	BE11554_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-119	Cytb	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	ACCCCTCCTCATATCCAACCA	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11554_Mgig_Bot2012_COI	DRWA-119	1	BE11554_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	TCTTATTCGTTGGAGACTTTATAACCCT	130.90649
BE11554	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50739	WGS84	BE11554	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11554_Mjap_Gib2022_COI	DRWA-119	1	BE11554_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11554	Sample	BE11554	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-119	COI	45	BE11554	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-119	AATTTAATTGTGACTAGGCATGGTCTG	130.90649
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11555_Cmae_Bot2011_COI	DRWA-120	1	BE11555_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	ATGAACAGTCTATCCTCCTTTAG	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11555_Hsan_Wil2021_COI	DRWA-120	1	BE11555_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	CCTGGGCCGGTATAGTAGGT	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11555_Mros_Gib2022_COI	DRWA-120	1	BE11555_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11555_Msal_Bot2012_COI	DRWA-120	1	BE11555_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	GYTAGTTCCRATGATGTTAGCTG	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11555_Mstr_Wil2021_COI	DRWA-120	1	BE11555_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	GTAGTGGTAACTACTCATGCTTTA	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11555_Pvir_Dia2013_COI	DRWA-120	1	BE11555_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	CTTAGTGGCATTAATTCGDAATCC	130.90767
BE11555	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5074	WGS84	BE11555	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11555_Rhar_Sim2018_COI	DRWA-120	1	BE11555_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11555	Sample	BE11555	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-120	COI	45	BE11555	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-120	CTGCTGCTATTGCTCATG	130.90767
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11557_Aamu_Bax2006_COI	DRWA-122	1	BE11557_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	GCACAACCGGGATCTTTACTTC	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11557_Pcan_Bot2011_IGS	DRWA-122	1	BE11557_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-122	IGS	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	CGTAATCCTCAGTACTGGCTA	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11557_Pper_Dia2013_COI	DRWA-122	1	BE11557_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	CTTAGTGGCATTAATTCGDAATCC	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11557_Sspa_Oph2007_COI	DRWA-122	1	BE11557_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	GAAGGCCTTCCGATGATC	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11557_Upin_Bot2011_COI	DRWA-122	1	BE11557_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	CTTTAATTACAGCGTTTTTATTGTTGT	130.91004
BE11557	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11557	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11557_Vgib_Wil2025_COI	DRWA-122	1	BE11557_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11557	Sample	BE11557	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-122	COI	45	BE11557	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-122	CCCCTCTTTACAAGTTCTTTAGGGT	130.91004
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11558_Asen_Wil2025_COI	DRWA-123	1	BE11558_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	GTTGTTGTTACAACACATGCTCTTTTG	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11558_Cjap_Sim2018_COI	DRWA-123	1	BE11558_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11558_Esin_And2018_Cyb	DRWA-123	1	BE11558_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-123	Cytb	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	ACCCCTCCTCATATCCAACCA	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11558_Mgig_Bot2012_COI	DRWA-123	1	BE11558_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	TCTTATTCGTTGGAGACTTTATAACCCT	130.9111
BE11558	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50748	WGS84	BE11558	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11558_Mjap_Gib2022_COI	DRWA-123	1	BE11558_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11558	Sample	BE11558	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-123	COI	45	BE11558	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-123	AATTTAATTGTGACTAGGCATGGTCTG	130.9111
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11559_Cmae_Bot2011_COI	DRWA-124	1	BE11559_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	ATGAACAGTCTATCCTCCTTTAG	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11559_Hsan_Wil2021_COI	DRWA-124	1	BE11559_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	CCTGGGCCGGTATAGTAGGT	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11559_Mros_Gib2022_COI	DRWA-124	1	BE11559_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11559_Msal_Bot2012_COI	DRWA-124	1	BE11559_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	GYTAGTTCCRATGATGTTAGCTG	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11559_Mstr_Wil2021_COI	DRWA-124	1	BE11559_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	GTAGTGGTAACTACTCATGCTTTA	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11559_Pvir_Dia2013_COI	DRWA-124	1	BE11559_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	CTTAGTGGCATTAATTCGDAATCC	130.91247
BE11559	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50753	WGS84	BE11559	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11559_Rhar_Sim2018_COI	DRWA-124	1	BE11559_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11559	Sample	BE11559	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-124	COI	45	BE11559	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-124	CTGCTGCTATTGCTCATG	130.91247
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11560_Aamu_Bax2006_COI	DRWA-125	1	BE11560_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	GCACAACCGGGATCTTTACTTC	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11560_Pcan_Bot2011_IGS	DRWA-125	1	BE11560_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-125	IGS	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	CGTAATCCTCAGTACTGGCTA	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11560_Pper_Dia2013_COI	DRWA-125	1	BE11560_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	CTTAGTGGCATTAATTCGDAATCC	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11560_Sspa_Oph2007_COI	DRWA-125	1	BE11560_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	GAAGGCCTTCCGATGATC	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11560_Upin_Bot2011_COI	DRWA-125	1	BE11560_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	CTTTAATTACAGCGTTTTTATTGTTGT	130.91337
BE11560	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50746	WGS84	BE11560	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11560_Vgib_Wil2025_COI	DRWA-125	1	BE11560_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11560	Sample	BE11560	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-125	COI	45	BE11560	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-125	CCCCTCTTTACAAGTTCTTTAGGGT	130.91337
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11561_Asen_Wil2025_COI	DRWA-126	1	BE11561_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	GTTGTTGTTACAACACATGCTCTTTTG	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11561_Cjap_Sim2018_COI	DRWA-126	1	BE11561_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11561_Esin_And2018_Cyb	DRWA-126	1	BE11561_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-126	Cytb	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	ACCCCTCCTCATATCCAACCA	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11561_Mgig_Bot2012_COI	DRWA-126	1	BE11561_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	TCTTATTCGTTGGAGACTTTATAACCCT	130.90563
BE11561	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5065	WGS84	BE11561	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11561_Mjap_Gib2022_COI	DRWA-126	1	BE11561_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11561	Sample	BE11561	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-126	COI	45	BE11561	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-126	AATTTAATTGTGACTAGGCATGGTCTG	130.90563
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11562_Cmae_Bot2011_COI	DRWA-127	1	BE11562_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	ATGAACAGTCTATCCTCCTTTAG	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11562_Hsan_Wil2021_COI	DRWA-127	1	BE11562_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	CCTGGGCCGGTATAGTAGGT	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11562_Mros_Gib2022_COI	DRWA-127	1	BE11562_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11562_Msal_Bot2012_COI	DRWA-127	1	BE11562_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	GYTAGTTCCRATGATGTTAGCTG	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11562_Mstr_Wil2021_COI	DRWA-127	1	BE11562_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	GTAGTGGTAACTACTCATGCTTTA	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11562_Pvir_Dia2013_COI	DRWA-127	1	BE11562_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	CTTAGTGGCATTAATTCGDAATCC	130.90671
BE11562	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50656	WGS84	BE11562	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11562_Rhar_Sim2018_COI	DRWA-127	1	BE11562_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11562	Sample	BE11562	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-127	COI	45	BE11562	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-127	CTGCTGCTATTGCTCATG	130.90671
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11564_Aamu_Bax2006_COI	DRWA-129	1	BE11564_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	GCACAACCGGGATCTTTACTTC	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11564_Pcan_Bot2011_IGS	DRWA-129	1	BE11564_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-129	IGS	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	CGTAATCCTCAGTACTGGCTA	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11564_Pper_Dia2013_COI	DRWA-129	1	BE11564_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	CTTAGTGGCATTAATTCGDAATCC	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11564_Sspa_Oph2007_COI	DRWA-129	1	BE11564_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	GAAGGCCTTCCGATGATC	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11564_Upin_Bot2011_COI	DRWA-129	1	BE11564_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	CTTTAATTACAGCGTTTTTATTGTTGT	130.90933
BE11564	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50647	WGS84	BE11564	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11564_Vgib_Wil2025_COI	DRWA-129	1	BE11564_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11564	Sample	BE11564	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-129	COI	45	BE11564	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-129	CCCCTCTTTACAAGTTCTTTAGGGT	130.90933
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11565_Asen_Wil2025_COI	DRWA-130	1	BE11565_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	GTTGTTGTTACAACACATGCTCTTTTG	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11565_Cjap_Sim2018_COI	DRWA-130	1	BE11565_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11565_Esin_And2018_Cyb	DRWA-130	1	BE11565_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-130	Cytb	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	ACCCCTCCTCATATCCAACCA	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11565_Mgig_Bot2012_COI	DRWA-130	1	BE11565_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	TCTTATTCGTTGGAGACTTTATAACCCT	130.91053
BE11565	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50649	WGS84	BE11565	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11565_Mjap_Gib2022_COI	DRWA-130	1	BE11565_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11565	Sample	BE11565	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-130	COI	45	BE11565	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-130	AATTTAATTGTGACTAGGCATGGTCTG	130.91053
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11566_Cmae_Bot2011_COI	DRWA-131	1	BE11566_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	ATGAACAGTCTATCCTCCTTTAG	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11566_Hsan_Wil2021_COI	DRWA-131	1	BE11566_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	CCTGGGCCGGTATAGTAGGT	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11566_Mros_Gib2022_COI	DRWA-131	1	BE11566_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11566_Msal_Bot2012_COI	DRWA-131	1	BE11566_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	GYTAGTTCCRATGATGTTAGCTG	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11566_Mstr_Wil2021_COI	DRWA-131	1	BE11566_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	GTAGTGGTAACTACTCATGCTTTA	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11566_Pvir_Dia2013_COI	DRWA-131	1	BE11566_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	CTTAGTGGCATTAATTCGDAATCC	130.91196
BE11566	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50655	WGS84	BE11566	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11566_Rhar_Sim2018_COI	DRWA-131	1	BE11566_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11566	Sample	BE11566	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-131	COI	45	BE11566	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-131	CTGCTGCTATTGCTCATG	130.91196
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11568_Aamu_Bax2006_COI	DRWA-133	1	BE11568_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	GCACAACCGGGATCTTTACTTC	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11568_Pcan_Bot2011_IGS	DRWA-133	1	BE11568_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-133	IGS	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	CGTAATCCTCAGTACTGGCTA	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11568_Pper_Dia2013_COI	DRWA-133	1	BE11568_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	CTTAGTGGCATTAATTCGDAATCC	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11568_Sspa_Oph2007_COI	DRWA-133	1	BE11568_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	GAAGGCCTTCCGATGATC	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11568_Upin_Bot2011_COI	DRWA-133	1	BE11568_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	CTTTAATTACAGCGTTTTTATTGTTGT	130.91413
BE11568	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50657	WGS84	BE11568	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11568_Vgib_Wil2025_COI	DRWA-133	1	BE11568_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11568	Sample	BE11568	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-133	COI	45	BE11568	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-133	CCCCTCTTTACAAGTTCTTTAGGGT	130.91413
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11569_Asen_Wil2025_COI	DRWA-134	1	BE11569_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	GTTGTTGTTACAACACATGCTCTTTTG	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11569_Cjap_Sim2018_COI	DRWA-134	1	BE11569_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11569_Esin_And2018_Cyb	DRWA-134	1	BE11569_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-134	Cytb	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	ACCCCTCCTCATATCCAACCA	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11569_Mgig_Bot2012_COI	DRWA-134	1	BE11569_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	TCTTATTCGTTGGAGACTTTATAACCCT	130.91543
BE11569	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50664	WGS84	BE11569	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11569_Mjap_Gib2022_COI	DRWA-134	1	BE11569_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11569	Sample	BE11569	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-134	COI	45	BE11569	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-134	AATTTAATTGTGACTAGGCATGGTCTG	130.91543
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11571_Aamu_Bax2006_COI	DRWA-136	1	BE11571_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	GCACAACCGGGATCTTTACTTC	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11571_Pcan_Bot2011_IGS	DRWA-136	1	BE11571_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-136	IGS	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	CGTAATCCTCAGTACTGGCTA	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11571_Pper_Dia2013_COI	DRWA-136	1	BE11571_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	CTTAGTGGCATTAATTCGDAATCC	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11571_Sspa_Oph2007_COI	DRWA-136	1	BE11571_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	GAAGGCCTTCCGATGATC	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11571_Upin_Bot2011_COI	DRWA-136	1	BE11571_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	CTTTAATTACAGCGTTTTTATTGTTGT	130.91023
BE11571	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50539	WGS84	BE11571	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11571_Vgib_Wil2025_COI	DRWA-136	1	BE11571_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11571	Sample	BE11571	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-136	COI	45	BE11571	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-136	CCCCTCTTTACAAGTTCTTTAGGGT	130.91023
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11572_Asen_Wil2025_COI	DRWA-137	1	BE11572_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	GTTGTTGTTACAACACATGCTCTTTTG	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11572_Cjap_Sim2018_COI	DRWA-137	1	BE11572_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11572_Esin_And2018_Cyb	DRWA-137	1	BE11572_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-137	Cytb	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	ACCCCTCCTCATATCCAACCA	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11572_Mgig_Bot2012_COI	DRWA-137	1	BE11572_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	TCTTATTCGTTGGAGACTTTATAACCCT	130.91144
BE11572	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11572	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11572_Mjap_Gib2022_COI	DRWA-137	1	BE11572_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11572	Sample	BE11572	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-137	COI	45	BE11572	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-137	AATTTAATTGTGACTAGGCATGGTCTG	130.91144
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11573_Cmae_Bot2011_COI	DRWA-138	1	BE11573_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	ATGAACAGTCTATCCTCCTTTAG	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11573_Hsan_Wil2021_COI	DRWA-138	1	BE11573_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	CCTGGGCCGGTATAGTAGGT	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11573_Mros_Gib2022_COI	DRWA-138	1	BE11573_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11573_Msal_Bot2012_COI	DRWA-138	1	BE11573_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	GYTAGTTCCRATGATGTTAGCTG	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11573_Mstr_Wil2021_COI	DRWA-138	1	BE11573_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	GTAGTGGTAACTACTCATGCTTTA	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11573_Pvir_Dia2013_COI	DRWA-138	1	BE11573_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	CTTAGTGGCATTAATTCGDAATCC	130.91245
BE11573	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.50552	WGS84	BE11573	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11573_Rhar_Sim2018_COI	DRWA-138	1	BE11573_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11573	Sample	BE11573	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-138	COI	45	BE11573	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-138	CTGCTGCTATTGCTCATG	130.91245
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11575_Aamu_Bax2006_COI	DRWA-140	1	BE11575_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	GCACAACCGGGATCTTTACTTC	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11575_Pcan_Bot2011_IGS	DRWA-140	1	BE11575_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-140	IGS	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	CGTAATCCTCAGTACTGGCTA	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11575_Pper_Dia2013_COI	DRWA-140	1	BE11575_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	CTTAGTGGCATTAATTCGDAATCC	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11575_Sspa_Oph2007_COI	DRWA-140	1	BE11575_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	GAAGGCCTTCCGATGATC	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11575_Upin_Bot2011_COI	DRWA-140	1	BE11575_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	CTTTAATTACAGCGTTTTTATTGTTGT	130.91305
BE11575	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16039	-12.5045	WGS84	BE11575	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_A	11/09/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11575_Vgib_Wil2025_COI	DRWA-140	1	BE11575_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11575	Sample	BE11575	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-140	COI	45	BE11575	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-140	CCCCTCTTTACAAGTTCTTTAGGGT	130.91305
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11580_Cmae_Bot2011_COI	DRWA-036	1	BE11580_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	ATGAACAGTCTATCCTCCTTTAG	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11580_Hsan_Wil2021_COI	DRWA-036	1	BE11580_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	CCTGGGCCGGTATAGTAGGT	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11580_Mros_Gib2022_COI	DRWA-036	1	BE11580_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11580_Msal_Bot2012_COI	DRWA-036	1	BE11580_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	GYTAGTTCCRATGATGTTAGCTG	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11580_Mstr_Wil2021_COI	DRWA-036	1	BE11580_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	GTAGTGGTAACTACTCATGCTTTA	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11580_Pvir_Dia2013_COI	DRWA-036	1	BE11580_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	CTTAGTGGCATTAATTCGDAATCC	130.93098
BE11580	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51532	WGS84	BE11580	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11580_Rhar_Sim2018_COI	DRWA-036	1	BE11580_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11580	Sample	BE11580	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-036	COI	45	BE11580	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-036	CTGCTGCTATTGCTCATG	130.93098
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11582_Aamu_Bax2006_COI	DRWA-038	1	BE11582_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	GCACAACCGGGATCTTTACTTC	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11582_Pcan_Bot2011_IGS	DRWA-038	1	BE11582_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-038	IGS	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	CGTAATCCTCAGTACTGGCTA	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11582_Pper_Dia2013_COI	DRWA-038	1	BE11582_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	CTTAGTGGCATTAATTCGDAATCC	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11582_Sspa_Oph2007_COI	DRWA-038	1	BE11582_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	GAAGGCCTTCCGATGATC	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11582_Upin_Bot2011_COI	DRWA-038	1	BE11582_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	CTTTAATTACAGCGTTTTTATTGTTGT	130.93065
BE11582	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51448	WGS84	BE11582	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11582_Vgib_Wil2025_COI	DRWA-038	1	BE11582_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11582	Sample	BE11582	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-038	COI	45	BE11582	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-038	CCCCTCTTTACAAGTTCTTTAGGGT	130.93065
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11583_Asen_Wil2025_COI	DRWA-039	1	BE11583_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	GTTGTTGTTACAACACATGCTCTTTTG	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11583_Cjap_Sim2018_COI	DRWA-039	1	BE11583_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11583_Esin_And2018_Cyb	DRWA-039	1	BE11583_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-039	Cytb	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	ACCCCTCCTCATATCCAACCA	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11583_Mgig_Bot2012_COI	DRWA-039	1	BE11583_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	TCTTATTCGTTGGAGACTTTATAACCCT	130.93139
BE11583	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51457	WGS84	BE11583	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11583_Mjap_Gib2022_COI	DRWA-039	1	BE11583_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11583	Sample	BE11583	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-039	COI	45	BE11583	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-039	AATTTAATTGTGACTAGGCATGGTCTG	130.93139
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11584_Cmae_Bot2011_COI	DRWA-040	1	BE11584_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	ATGAACAGTCTATCCTCCTTTAG	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11584_Hsan_Wil2021_COI	DRWA-040	1	BE11584_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	CCTGGGCCGGTATAGTAGGT	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11584_Mros_Gib2022_COI	DRWA-040	1	BE11584_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11584_Msal_Bot2012_COI	DRWA-040	1	BE11584_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	GYTAGTTCCRATGATGTTAGCTG	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11584_Mstr_Wil2021_COI	DRWA-040	1	BE11584_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	GTAGTGGTAACTACTCATGCTTTA	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11584_Pvir_Dia2013_COI	DRWA-040	1	BE11584_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	CTTAGTGGCATTAATTCGDAATCC	130.92998
BE11584	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51362	WGS84	BE11584	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11584_Rhar_Sim2018_COI	DRWA-040	1	BE11584_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11584	Sample	BE11584	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-040	COI	45	BE11584	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-040	CTGCTGCTATTGCTCATG	130.92998
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11586_Aamu_Bax2006_COI	DRWA-042	1	BE11586_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	GCACAACCGGGATCTTTACTTC	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11586_Pcan_Bot2011_IGS	DRWA-042	1	BE11586_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-042	IGS	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	CGTAATCCTCAGTACTGGCTA	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11586_Pper_Dia2013_COI	DRWA-042	1	BE11586_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	CTTAGTGGCATTAATTCGDAATCC	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11586_Sspa_Oph2007_COI	DRWA-042	1	BE11586_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	GAAGGCCTTCCGATGATC	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11586_Upin_Bot2011_COI	DRWA-042	1	BE11586_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	CTTTAATTACAGCGTTTTTATTGTTGT	130.93261
BE11586	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51394	WGS84	BE11586	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11586_Vgib_Wil2025_COI	DRWA-042	1	BE11586_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11586	Sample	BE11586	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-042	COI	45	BE11586	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-042	CCCCTCTTTACAAGTTCTTTAGGGT	130.93261
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11587_Asen_Wil2025_COI	DRWA-043	1	BE11587_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	GTTGTTGTTACAACACATGCTCTTTTG	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11587_Cjap_Sim2018_COI	DRWA-043	1	BE11587_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11587_Esin_And2018_Cyb	DRWA-043	1	BE11587_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-043	Cytb	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	ACCCCTCCTCATATCCAACCA	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11587_Mgig_Bot2012_COI	DRWA-043	1	BE11587_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	TCTTATTCGTTGGAGACTTTATAACCCT	130.9301
BE11587	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51313	WGS84	BE11587	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11587_Mjap_Gib2022_COI	DRWA-043	1	BE11587_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11587	Sample	BE11587	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-043	COI	45	BE11587	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-043	AATTTAATTGTGACTAGGCATGGTCTG	130.9301
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11588_Cmae_Bot2011_COI	DRWA-044	1	BE11588_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	ATGAACAGTCTATCCTCCTTTAG	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11588_Hsan_Wil2021_COI	DRWA-044	1	BE11588_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	CCTGGGCCGGTATAGTAGGT	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11588_Mros_Gib2022_COI	DRWA-044	1	BE11588_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11588_Msal_Bot2012_COI	DRWA-044	1	BE11588_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	GYTAGTTCCRATGATGTTAGCTG	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11588_Mstr_Wil2021_COI	DRWA-044	1	BE11588_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	GTAGTGGTAACTACTCATGCTTTA	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11588_Pvir_Dia2013_COI	DRWA-044	1	BE11588_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	CTTAGTGGCATTAATTCGDAATCC	130.93111
BE11588	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51312	WGS84	BE11588	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11588_Rhar_Sim2018_COI	DRWA-044	1	BE11588_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11588	Sample	BE11588	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-044	COI	45	BE11588	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-044	CTGCTGCTATTGCTCATG	130.93111
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11590_Asen_Wil2025_COI	DRWA-046	1	BE11590_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	GTTGTTGTTACAACACATGCTCTTTTG	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11590_Cjap_Sim2018_COI	DRWA-046	1	BE11590_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11590_Esin_And2018_Cyb	DRWA-046	1	BE11590_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-046	Cytb	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	ACCCCTCCTCATATCCAACCA	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11590_Mgig_Bot2012_COI	DRWA-046	1	BE11590_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	TCTTATTCGTTGGAGACTTTATAACCCT	130.92965
BE11590	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51215	WGS84	BE11590	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11590_Mjap_Gib2022_COI	DRWA-046	1	BE11590_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11590	Sample	BE11590	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-046	COI	45	BE11590	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-046	AATTTAATTGTGACTAGGCATGGTCTG	130.92965
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11591_Cmae_Bot2011_COI	DRWA-047	1	BE11591_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	ATGAACAGTCTATCCTCCTTTAG	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11591_Hsan_Wil2021_COI	DRWA-047	1	BE11591_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	CCTGGGCCGGTATAGTAGGT	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11591_Mros_Gib2022_COI	DRWA-047	1	BE11591_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11591_Msal_Bot2012_COI	DRWA-047	1	BE11591_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	GYTAGTTCCRATGATGTTAGCTG	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11591_Mstr_Wil2021_COI	DRWA-047	1	BE11591_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	GTAGTGGTAACTACTCATGCTTTA	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11591_Pvir_Dia2013_COI	DRWA-047	1	BE11591_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	CTTAGTGGCATTAATTCGDAATCC	130.93074
BE11591	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51216	WGS84	BE11591	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11591_Rhar_Sim2018_COI	DRWA-047	1	BE11591_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11591	Sample	BE11591	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-047	COI	45	BE11591	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-047	CTGCTGCTATTGCTCATG	130.93074
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11593_Aamu_Bax2006_COI	DRWA-049	1	BE11593_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	GCACAACCGGGATCTTTACTTC	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11593_Pcan_Bot2011_IGS	DRWA-049	1	BE11593_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-049	IGS	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	CGTAATCCTCAGTACTGGCTA	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11593_Pper_Dia2013_COI	DRWA-049	1	BE11593_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	CTTAGTGGCATTAATTCGDAATCC	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11593_Sspa_Oph2007_COI	DRWA-049	1	BE11593_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	GAAGGCCTTCCGATGATC	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11593_Upin_Bot2011_COI	DRWA-049	1	BE11593_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	CTTTAATTACAGCGTTTTTATTGTTGT	130.92916
BE11593	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.5113	WGS84	BE11593	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11593_Vgib_Wil2025_COI	DRWA-049	1	BE11593_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11593	Sample	BE11593	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-049	COI	45	BE11593	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-049	CCCCTCTTTACAAGTTCTTTAGGGT	130.92916
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11594_Asen_Wil2025_COI	DRWA-050	1	BE11594_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	GTTGTTGTTACAACACATGCTCTTTTG	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11594_Cjap_Sim2018_COI	DRWA-050	1	BE11594_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11594_Esin_And2018_Cyb	DRWA-050	1	BE11594_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-050	Cytb	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	ACCCCTCCTCATATCCAACCA	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11594_Mgig_Bot2012_COI	DRWA-050	1	BE11594_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	TCTTATTCGTTGGAGACTTTATAACCCT	130.93018
BE11594	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51127	WGS84	BE11594	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11594_Mjap_Gib2022_COI	DRWA-050	1	BE11594_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11594	Sample	BE11594	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-050	COI	45	BE11594	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-050	AATTTAATTGTGACTAGGCATGGTCTG	130.93018
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11595_Cmae_Bot2011_COI	DRWA-051	1	BE11595_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	ATGAACAGTCTATCCTCCTTTAG	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11595_Hsan_Wil2021_COI	DRWA-051	1	BE11595_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	CCTGGGCCGGTATAGTAGGT	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11595_Mros_Gib2022_COI	DRWA-051	1	BE11595_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11595_Msal_Bot2012_COI	DRWA-051	1	BE11595_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	GYTAGTTCCRATGATGTTAGCTG	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11595_Mstr_Wil2021_COI	DRWA-051	1	BE11595_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	GTAGTGGTAACTACTCATGCTTTA	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11595_Pvir_Dia2013_COI	DRWA-051	1	BE11595_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	CTTAGTGGCATTAATTCGDAATCC	130.93119
BE11595	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51146	WGS84	BE11595	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11595_Rhar_Sim2018_COI	DRWA-051	1	BE11595_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11595	Sample	BE11595	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-051	COI	45	BE11595	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-051	CTGCTGCTATTGCTCATG	130.93119
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11597_Aamu_Bax2006_COI	DRWA-053	1	BE11597_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	GCACAACCGGGATCTTTACTTC	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11597_Pcan_Bot2011_IGS	DRWA-053	1	BE11597_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-053	IGS	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	CGTAATCCTCAGTACTGGCTA	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11597_Pper_Dia2013_COI	DRWA-053	1	BE11597_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	CTTAGTGGCATTAATTCGDAATCC	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11597_Sspa_Oph2007_COI	DRWA-053	1	BE11597_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	GAAGGCCTTCCGATGATC	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11597_Upin_Bot2011_COI	DRWA-053	1	BE11597_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	CTTTAATTACAGCGTTTTTATTGTTGT	130.9286
BE11597	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51035	WGS84	BE11597	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11597_Vgib_Wil2025_COI	DRWA-053	1	BE11597_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11597	Sample	BE11597	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-053	COI	45	BE11597	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-053	CCCCTCTTTACAAGTTCTTTAGGGT	130.9286
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11598_Asen_Wil2025_COI	DRWA-054	1	BE11598_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	GTTGTTGTTACAACACATGCTCTTTTG	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11598_Cjap_Sim2018_COI	DRWA-054	1	BE11598_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11598_Esin_And2018_Cyb	DRWA-054	1	BE11598_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-054	Cytb	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	ACCCCTCCTCATATCCAACCA	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11598_Mgig_Bot2012_COI	DRWA-054	1	BE11598_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	TCTTATTCGTTGGAGACTTTATAACCCT	130.92966
BE11598	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51045	WGS84	BE11598	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11598_Mjap_Gib2022_COI	DRWA-054	1	BE11598_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11598	Sample	BE11598	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-054	COI	45	BE11598	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-054	AATTTAATTGTGACTAGGCATGGTCTG	130.92966
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11599_Cmae_Bot2011_COI	DRWA-055	1	BE11599_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	ATGAACAGTCTATCCTCCTTTAG	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11599_Hsan_Wil2021_COI	DRWA-055	1	BE11599_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	CCTGGGCCGGTATAGTAGGT	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11599_Mros_Gib2022_COI	DRWA-055	1	BE11599_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11599_Msal_Bot2012_COI	DRWA-055	1	BE11599_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	GYTAGTTCCRATGATGTTAGCTG	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11599_Mstr_Wil2021_COI	DRWA-055	1	BE11599_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	GTAGTGGTAACTACTCATGCTTTA	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11599_Pvir_Dia2013_COI	DRWA-055	1	BE11599_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	CTTAGTGGCATTAATTCGDAATCC	130.93069
BE11599	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.51039	WGS84	BE11599	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11599_Rhar_Sim2018_COI	DRWA-055	1	BE11599_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11599	Sample	BE11599	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-055	COI	45	BE11599	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-055	CTGCTGCTATTGCTCATG	130.93069
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11601_Aamu_Bax2006_COI	DRWA-057	1	BE11601_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	GCACAACCGGGATCTTTACTTC	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11601_Pcan_Bot2011_IGS	DRWA-057	1	BE11601_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-057	IGS	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	CGTAATCCTCAGTACTGGCTA	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11601_Pper_Dia2013_COI	DRWA-057	1	BE11601_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	CTTAGTGGCATTAATTCGDAATCC	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11601_Sspa_Oph2007_COI	DRWA-057	1	BE11601_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	GAAGGCCTTCCGATGATC	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11601_Upin_Bot2011_COI	DRWA-057	1	BE11601_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	CTTTAATTACAGCGTTTTTATTGTTGT	130.92821
BE11601	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50952	WGS84	BE11601	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11601_Vgib_Wil2025_COI	DRWA-057	1	BE11601_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11601	Sample	BE11601	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-057	COI	45	BE11601	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-057	CCCCTCTTTACAAGTTCTTTAGGGT	130.92821
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11602_Asen_Wil2025_COI	DRWA-058	1	BE11602_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	GTTGTTGTTACAACACATGCTCTTTTG	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11602_Cjap_Sim2018_COI	DRWA-058	1	BE11602_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11602_Esin_And2018_Cyb	DRWA-058	1	BE11602_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-058	Cytb	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	ACCCCTCCTCATATCCAACCA	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11602_Mgig_Bot2012_COI	DRWA-058	1	BE11602_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	TCTTATTCGTTGGAGACTTTATAACCCT	130.92919
BE11602	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50958	WGS84	BE11602	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11602_Mjap_Gib2022_COI	DRWA-058	1	BE11602_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11602	Sample	BE11602	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-058	COI	45	BE11602	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-058	AATTTAATTGTGACTAGGCATGGTCTG	130.92919
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11603_Cmae_Bot2011_COI	DRWA-059	1	BE11603_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	ATGAACAGTCTATCCTCCTTTAG	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11603_Hsan_Wil2021_COI	DRWA-059	1	BE11603_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	CCTGGGCCGGTATAGTAGGT	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11603_Mros_Gib2022_COI	DRWA-059	1	BE11603_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11603_Msal_Bot2012_COI	DRWA-059	1	BE11603_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	GYTAGTTCCRATGATGTTAGCTG	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11603_Mstr_Wil2021_COI	DRWA-059	1	BE11603_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	GTAGTGGTAACTACTCATGCTTTA	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11603_Pvir_Dia2013_COI	DRWA-059	1	BE11603_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	CTTAGTGGCATTAATTCGDAATCC	130.93037
BE11603	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50954	WGS84	BE11603	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11603_Rhar_Sim2018_COI	DRWA-059	1	BE11603_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11603	Sample	BE11603	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-059	COI	45	BE11603	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-059	CTGCTGCTATTGCTCATG	130.93037
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11605_Aamu_Bax2006_COI	DRWA-061	1	BE11605_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	GCACAACCGGGATCTTTACTTC	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11605_Pcan_Bot2011_IGS	DRWA-061	1	BE11605_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-061	IGS	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	CGTAATCCTCAGTACTGGCTA	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11605_Pper_Dia2013_COI	DRWA-061	1	BE11605_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	CTTAGTGGCATTAATTCGDAATCC	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11605_Sspa_Oph2007_COI	DRWA-061	1	BE11605_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	GAAGGCCTTCCGATGATC	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11605_Upin_Bot2011_COI	DRWA-061	1	BE11605_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	CTTTAATTACAGCGTTTTTATTGTTGT	130.92762
BE11605	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50866	WGS84	BE11605	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11605_Vgib_Wil2025_COI	DRWA-061	1	BE11605_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11605	Sample	BE11605	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-061	COI	45	BE11605	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-061	CCCCTCTTTACAAGTTCTTTAGGGT	130.92762
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11606_Asen_Wil2025_COI	DRWA-062	1	BE11606_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	GTTGTTGTTACAACACATGCTCTTTTG	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11606_Cjap_Sim2018_COI	DRWA-062	1	BE11606_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11606_Esin_And2018_Cyb	DRWA-062	1	BE11606_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-062	Cytb	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	ACCCCTCCTCATATCCAACCA	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11606_Mgig_Bot2012_COI	DRWA-062	1	BE11606_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	TCTTATTCGTTGGAGACTTTATAACCCT	130.92868
BE11606	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50864	WGS84	BE11606	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11606_Mjap_Gib2022_COI	DRWA-062	1	BE11606_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11606	Sample	BE11606	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-062	COI	45	BE11606	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-062	AATTTAATTGTGACTAGGCATGGTCTG	130.92868
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11607_Cmae_Bot2011_COI	DRWA-063	1	BE11607_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	ATGAACAGTCTATCCTCCTTTAG	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11607_Hsan_Wil2021_COI	DRWA-063	1	BE11607_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	CCTGGGCCGGTATAGTAGGT	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11607_Mros_Gib2022_COI	DRWA-063	1	BE11607_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11607_Msal_Bot2012_COI	DRWA-063	1	BE11607_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	GYTAGTTCCRATGATGTTAGCTG	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11607_Mstr_Wil2021_COI	DRWA-063	1	BE11607_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	GTAGTGGTAACTACTCATGCTTTA	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11607_Pvir_Dia2013_COI	DRWA-063	1	BE11607_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	CTTAGTGGCATTAATTCGDAATCC	130.92967
BE11607	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50861	WGS84	BE11607	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11607_Rhar_Sim2018_COI	DRWA-063	1	BE11607_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11607	Sample	BE11607	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-063	COI	45	BE11607	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-063	CTGCTGCTATTGCTCATG	130.92967
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11609_Aamu_Bax2006_COI	DRWA-065	1	BE11609_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	GCACAACCGGGATCTTTACTTC	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11609_Pcan_Bot2011_IGS	DRWA-065	1	BE11609_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-065	IGS	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	CGTAATCCTCAGTACTGGCTA	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11609_Pper_Dia2013_COI	DRWA-065	1	BE11609_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	CTTAGTGGCATTAATTCGDAATCC	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11609_Sspa_Oph2007_COI	DRWA-065	1	BE11609_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	GAAGGCCTTCCGATGATC	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11609_Upin_Bot2011_COI	DRWA-065	1	BE11609_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	CTTTAATTACAGCGTTTTTATTGTTGT	130.92723
BE11609	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50786	WGS84	BE11609	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11609_Vgib_Wil2025_COI	DRWA-065	1	BE11609_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11609	Sample	BE11609	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-065	COI	45	BE11609	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-065	CCCCTCTTTACAAGTTCTTTAGGGT	130.92723
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11614_Cmae_Bot2011_COI	DRWA-066	1	BE11614_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	ATGAACAGTCTATCCTCCTTTAG	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11614_Hsan_Wil2021_COI	DRWA-066	1	BE11614_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	CCTGGGCCGGTATAGTAGGT	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11614_Mros_Gib2022_COI	DRWA-066	1	BE11614_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11614_Msal_Bot2012_COI	DRWA-066	1	BE11614_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	GYTAGTTCCRATGATGTTAGCTG	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11614_Mstr_Wil2021_COI	DRWA-066	1	BE11614_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	GTAGTGGTAACTACTCATGCTTTA	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11614_Pvir_Dia2013_COI	DRWA-066	1	BE11614_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	CTTAGTGGCATTAATTCGDAATCC	130.92805
BE11614	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50757	WGS84	BE11614	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11614_Rhar_Sim2018_COI	DRWA-066	1	BE11614_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11614	Sample	BE11614	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-066	COI	45	BE11614	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-066	CTGCTGCTATTGCTCATG	130.92805
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11616_Aamu_Bax2006_COI	DRWA-068	1	BE11616_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	GCACAACCGGGATCTTTACTTC	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11616_Pcan_Bot2011_IGS	DRWA-068	1	BE11616_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-068	IGS	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	CGTAATCCTCAGTACTGGCTA	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11616_Pper_Dia2013_COI	DRWA-068	1	BE11616_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	CTTAGTGGCATTAATTCGDAATCC	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11616_Sspa_Oph2007_COI	DRWA-068	1	BE11616_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	GAAGGCCTTCCGATGATC	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11616_Upin_Bot2011_COI	DRWA-068	1	BE11616_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	CTTTAATTACAGCGTTTTTATTGTTGT	130.93016
BE11616	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50759	WGS84	BE11616	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11616_Vgib_Wil2025_COI	DRWA-068	1	BE11616_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11616	Sample	BE11616	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-068	COI	45	BE11616	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-068	CCCCTCTTTACAAGTTCTTTAGGGT	130.93016
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11617_Asen_Wil2025_COI	DRWA-069	1	BE11617_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	GTTGTTGTTACAACACATGCTCTTTTG	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11617_Cjap_Sim2018_COI	DRWA-069	1	BE11617_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11617_Esin_And2018_Cyb	DRWA-069	1	BE11617_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-069	Cytb	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	ACCCCTCCTCATATCCAACCA	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11617_Mgig_Bot2012_COI	DRWA-069	1	BE11617_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	TCTTATTCGTTGGAGACTTTATAACCCT	130.92765
BE11617	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50665	WGS84	BE11617	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11617_Mjap_Gib2022_COI	DRWA-069	1	BE11617_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11617	Sample	BE11617	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-069	COI	45	BE11617	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-069	AATTTAATTGTGACTAGGCATGGTCTG	130.92765
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11618_Cmae_Bot2011_COI	DRWA-070	1	BE11618_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	ATGAACAGTCTATCCTCCTTTAG	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11618_Hsan_Wil2021_COI	DRWA-070	1	BE11618_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	CCTGGGCCGGTATAGTAGGT	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11618_Mros_Gib2022_COI	DRWA-070	1	BE11618_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11618_Msal_Bot2012_COI	DRWA-070	1	BE11618_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	GYTAGTTCCRATGATGTTAGCTG	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11618_Mstr_Wil2021_COI	DRWA-070	1	BE11618_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	GTAGTGGTAACTACTCATGCTTTA	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11618_Pvir_Dia2013_COI	DRWA-070	1	BE11618_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	CTTAGTGGCATTAATTCGDAATCC	130.92862
BE11618	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.50668	WGS84	BE11618	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_A	11/07/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11618_Rhar_Sim2018_COI	DRWA-070	1	BE11618_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11618	Sample	BE11618	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-070	COI	45	BE11618	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-070	CTGCTGCTATTGCTCATG	130.92862
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11620_Asen_Wil2025_COI	DRWA-072	1	BE11620_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	GTTGTTGTTACAACACATGCTCTTTTG	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11620_Cjap_Sim2018_COI	DRWA-072	1	BE11620_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11620_Esin_And2018_Cyb	DRWA-072	1	BE11620_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-072	Cytb	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	ACCCCTCCTCATATCCAACCA	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11620_Mgig_Bot2012_COI	DRWA-072	1	BE11620_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	TCTTATTCGTTGGAGACTTTATAACCCT	130.8886
BE11620	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49634	WGS84	BE11620	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11620_Mjap_Gib2022_COI	DRWA-072	1	BE11620_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11620	Sample	BE11620	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-072	COI	45	BE11620	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-072	AATTTAATTGTGACTAGGCATGGTCTG	130.8886
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11621_Cmae_Bot2011_COI	DRWA-073	1	BE11621_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	ATGAACAGTCTATCCTCCTTTAG	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11621_Hsan_Wil2021_COI	DRWA-073	1	BE11621_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	CCTGGGCCGGTATAGTAGGT	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11621_Mros_Gib2022_COI	DRWA-073	1	BE11621_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11621_Msal_Bot2012_COI	DRWA-073	1	BE11621_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	GYTAGTTCCRATGATGTTAGCTG	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11621_Mstr_Wil2021_COI	DRWA-073	1	BE11621_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	GTAGTGGTAACTACTCATGCTTTA	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11621_Pvir_Dia2013_COI	DRWA-073	1	BE11621_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	CTTAGTGGCATTAATTCGDAATCC	130.89041
BE11621	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49657	WGS84	BE11621	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11621_Rhar_Sim2018_COI	DRWA-073	1	BE11621_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11621	Sample	BE11621	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-073	COI	45	BE11621	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-073	CTGCTGCTATTGCTCATG	130.89041
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11623_Aamu_Bax2006_COI	DRWA-075	1	BE11623_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	GCACAACCGGGATCTTTACTTC	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11623_Pcan_Bot2011_IGS	DRWA-075	1	BE11623_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-075	IGS	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	CGTAATCCTCAGTACTGGCTA	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11623_Pper_Dia2013_COI	DRWA-075	1	BE11623_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	CTTAGTGGCATTAATTCGDAATCC	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11623_Sspa_Oph2007_COI	DRWA-075	1	BE11623_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	GAAGGCCTTCCGATGATC	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11623_Upin_Bot2011_COI	DRWA-075	1	BE11623_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	CTTTAATTACAGCGTTTTTATTGTTGT	130.87952
BE11623	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49493	WGS84	BE11623	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11623_Vgib_Wil2025_COI	DRWA-075	1	BE11623_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11623	Sample	BE11623	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-075	COI	45	BE11623	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-075	CCCCTCTTTACAAGTTCTTTAGGGT	130.87952
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11624_Asen_Wil2025_COI	DRWA-076	1	BE11624_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	GTTGTTGTTACAACACATGCTCTTTTG	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11624_Cjap_Sim2018_COI	DRWA-076	1	BE11624_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11624_Esin_And2018_Cyb	DRWA-076	1	BE11624_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-076	Cytb	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	ACCCCTCCTCATATCCAACCA	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11624_Mgig_Bot2012_COI	DRWA-076	1	BE11624_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	TCTTATTCGTTGGAGACTTTATAACCCT	130.88109
BE11624	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49512	WGS84	BE11624	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11624_Mjap_Gib2022_COI	DRWA-076	1	BE11624_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11624	Sample	BE11624	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-076	COI	45	BE11624	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-076	AATTTAATTGTGACTAGGCATGGTCTG	130.88109
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11625_Cmae_Bot2011_COI	DRWA-077	1	BE11625_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	ATGAACAGTCTATCCTCCTTTAG	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11625_Hsan_Wil2021_COI	DRWA-077	1	BE11625_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	CCTGGGCCGGTATAGTAGGT	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11625_Mros_Gib2022_COI	DRWA-077	1	BE11625_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11625_Msal_Bot2012_COI	DRWA-077	1	BE11625_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	GYTAGTTCCRATGATGTTAGCTG	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11625_Mstr_Wil2021_COI	DRWA-077	1	BE11625_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	GTAGTGGTAACTACTCATGCTTTA	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11625_Pvir_Dia2013_COI	DRWA-077	1	BE11625_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	CTTAGTGGCATTAATTCGDAATCC	130.88243
BE11625	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49514	WGS84	BE11625	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11625_Rhar_Sim2018_COI	DRWA-077	1	BE11625_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11625	Sample	BE11625	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-077	COI	45	BE11625	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-077	CTGCTGCTATTGCTCATG	130.88243
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11627_Aamu_Bax2006_COI	DRWA-079	1	BE11627_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	GCACAACCGGGATCTTTACTTC	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11627_Pcan_Bot2011_IGS	DRWA-079	1	BE11627_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-079	IGS	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	CGTAATCCTCAGTACTGGCTA	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11627_Pper_Dia2013_COI	DRWA-079	1	BE11627_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	CTTAGTGGCATTAATTCGDAATCC	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11627_Sspa_Oph2007_COI	DRWA-079	1	BE11627_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	GAAGGCCTTCCGATGATC	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11627_Upin_Bot2011_COI	DRWA-079	1	BE11627_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	CTTTAATTACAGCGTTTTTATTGTTGT	130.88853
BE11627	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11627	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11627_Vgib_Wil2025_COI	DRWA-079	1	BE11627_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11627	Sample	BE11627	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-079	COI	45	BE11627	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-079	CCCCTCTTTACAAGTTCTTTAGGGT	130.88853
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11628_Asen_Wil2025_COI	DRWA-080	1	BE11628_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	GTTGTTGTTACAACACATGCTCTTTTG	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11628_Cjap_Sim2018_COI	DRWA-080	1	BE11628_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11628_Esin_And2018_Cyb	DRWA-080	1	BE11628_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-080	Cytb	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	ACCCCTCCTCATATCCAACCA	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11628_Mgig_Bot2012_COI	DRWA-080	1	BE11628_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	TCTTATTCGTTGGAGACTTTATAACCCT	130.88966
BE11628	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49534	WGS84	BE11628	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11628_Mjap_Gib2022_COI	DRWA-080	1	BE11628_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11628	Sample	BE11628	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-080	COI	45	BE11628	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-080	AATTTAATTGTGACTAGGCATGGTCTG	130.88966
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11629_Cmae_Bot2011_COI	DRWA-081	1	BE11629_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	ATGAACAGTCTATCCTCCTTTAG	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11629_Hsan_Wil2021_COI	DRWA-081	1	BE11629_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	CCTGGGCCGGTATAGTAGGT	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11629_Mros_Gib2022_COI	DRWA-081	1	BE11629_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11629_Msal_Bot2012_COI	DRWA-081	1	BE11629_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	GYTAGTTCCRATGATGTTAGCTG	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11629_Mstr_Wil2021_COI	DRWA-081	1	BE11629_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	GTAGTGGTAACTACTCATGCTTTA	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11629_Pvir_Dia2013_COI	DRWA-081	1	BE11629_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	CTTAGTGGCATTAATTCGDAATCC	130.87884
BE11629	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49378	WGS84	BE11629	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11629_Rhar_Sim2018_COI	DRWA-081	1	BE11629_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11629	Sample	BE11629	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-081	COI	45	BE11629	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-081	CTGCTGCTATTGCTCATG	130.87884
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11630_Aamu_Bax2006_COI	DRWA-082	1	BE11630_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	GCACAACCGGGATCTTTACTTC	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11630_Pcan_Bot2011_IGS	DRWA-082	1	BE11630_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-082	IGS	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	CGTAATCCTCAGTACTGGCTA	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11630_Pper_Dia2013_COI	DRWA-082	1	BE11630_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	CTTAGTGGCATTAATTCGDAATCC	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11630_Sspa_Oph2007_COI	DRWA-082	1	BE11630_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	GAAGGCCTTCCGATGATC	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11630_Upin_Bot2011_COI	DRWA-082	1	BE11630_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	CTTTAATTACAGCGTTTTTATTGTTGT	130.88028
BE11630	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4939	WGS84	BE11630	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11630_Vgib_Wil2025_COI	DRWA-082	1	BE11630_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11630	Sample	BE11630	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-082	COI	45	BE11630	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-082	CCCCTCTTTACAAGTTCTTTAGGGT	130.88028
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11631_Asen_Wil2025_COI	DRWA-083	1	BE11631_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	GTTGTTGTTACAACACATGCTCTTTTG	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11631_Cjap_Sim2018_COI	DRWA-083	1	BE11631_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11631_Esin_And2018_Cyb	DRWA-083	1	BE11631_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-083	Cytb	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	ACCCCTCCTCATATCCAACCA	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11631_Mgig_Bot2012_COI	DRWA-083	1	BE11631_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	TCTTATTCGTTGGAGACTTTATAACCCT	130.88173
BE11631	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49388	WGS84	BE11631	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11631_Mjap_Gib2022_COI	DRWA-083	1	BE11631_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11631	Sample	BE11631	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-083	COI	45	BE11631	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-083	AATTTAATTGTGACTAGGCATGGTCTG	130.88173
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11632_Cmae_Bot2011_COI	DRWA-084	1	BE11632_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	ATGAACAGTCTATCCTCCTTTAG	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11632_Hsan_Wil2021_COI	DRWA-084	1	BE11632_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	CCTGGGCCGGTATAGTAGGT	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11632_Mros_Gib2022_COI	DRWA-084	1	BE11632_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11632_Msal_Bot2012_COI	DRWA-084	1	BE11632_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	GYTAGTTCCRATGATGTTAGCTG	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11632_Mstr_Wil2021_COI	DRWA-084	1	BE11632_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	GTAGTGGTAACTACTCATGCTTTA	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11632_Pvir_Dia2013_COI	DRWA-084	1	BE11632_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	CTTAGTGGCATTAATTCGDAATCC	130.87667
BE11632	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49253	WGS84	BE11632	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11632_Rhar_Sim2018_COI	DRWA-084	1	BE11632_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11632	Sample	BE11632	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-084	COI	45	BE11632	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-084	CTGCTGCTATTGCTCATG	130.87667
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11634_Aamu_Bax2006_COI	DRWA-086	1	BE11634_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	GCACAACCGGGATCTTTACTTC	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11634_Pcan_Bot2011_IGS	DRWA-086	1	BE11634_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-086	IGS	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	CGTAATCCTCAGTACTGGCTA	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11634_Pper_Dia2013_COI	DRWA-086	1	BE11634_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	CTTAGTGGCATTAATTCGDAATCC	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11634_Sspa_Oph2007_COI	DRWA-086	1	BE11634_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	GAAGGCCTTCCGATGATC	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11634_Upin_Bot2011_COI	DRWA-086	1	BE11634_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	CTTTAATTACAGCGTTTTTATTGTTGT	130.87958
BE11634	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49259	WGS84	BE11634	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11634_Vgib_Wil2025_COI	DRWA-086	1	BE11634_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11634	Sample	BE11634	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-086	COI	45	BE11634	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-086	CCCCTCTTTACAAGTTCTTTAGGGT	130.87958
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11635_Asen_Wil2025_COI	DRWA-087	1	BE11635_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	GTTGTTGTTACAACACATGCTCTTTTG	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11635_Cjap_Sim2018_COI	DRWA-087	1	BE11635_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11635_Esin_And2018_Cyb	DRWA-087	1	BE11635_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-087	Cytb	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	ACCCCTCCTCATATCCAACCA	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11635_Mgig_Bot2012_COI	DRWA-087	1	BE11635_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	TCTTATTCGTTGGAGACTTTATAACCCT	130.88106
BE11635	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49277	WGS84	BE11635	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11635_Mjap_Gib2022_COI	DRWA-087	1	BE11635_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11635	Sample	BE11635	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-087	COI	45	BE11635	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-087	AATTTAATTGTGACTAGGCATGGTCTG	130.88106
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11636_Cmae_Bot2011_COI	DRWA-088	1	BE11636_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	ATGAACAGTCTATCCTCCTTTAG	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11636_Hsan_Wil2021_COI	DRWA-088	1	BE11636_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	CCTGGGCCGGTATAGTAGGT	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11636_Mros_Gib2022_COI	DRWA-088	1	BE11636_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11636_Msal_Bot2012_COI	DRWA-088	1	BE11636_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	GYTAGTTCCRATGATGTTAGCTG	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11636_Mstr_Wil2021_COI	DRWA-088	1	BE11636_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	GTAGTGGTAACTACTCATGCTTTA	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11636_Pvir_Dia2013_COI	DRWA-088	1	BE11636_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	CTTAGTGGCATTAATTCGDAATCC	130.87464
BE11636	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49137	WGS84	BE11636	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11636_Rhar_Sim2018_COI	DRWA-088	1	BE11636_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11636	Sample	BE11636	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-088	COI	45	BE11636	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-088	CTGCTGCTATTGCTCATG	130.87464
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11638_Aamu_Bax2006_COI	DRWA-090	1	BE11638_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	GCACAACCGGGATCTTTACTTC	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11638_Pcan_Bot2011_IGS	DRWA-090	1	BE11638_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-090	IGS	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	CGTAATCCTCAGTACTGGCTA	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11638_Pper_Dia2013_COI	DRWA-090	1	BE11638_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	CTTAGTGGCATTAATTCGDAATCC	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11638_Sspa_Oph2007_COI	DRWA-090	1	BE11638_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	GAAGGCCTTCCGATGATC	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11638_Upin_Bot2011_COI	DRWA-090	1	BE11638_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	CTTTAATTACAGCGTTTTTATTGTTGT	130.87752
BE11638	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49146	WGS84	BE11638	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11638_Vgib_Wil2025_COI	DRWA-090	1	BE11638_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11638	Sample	BE11638	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-090	COI	45	BE11638	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-090	CCCCTCTTTACAAGTTCTTTAGGGT	130.87752
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11639_Asen_Wil2025_COI	DRWA-091	1	BE11639_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	GTTGTTGTTACAACACATGCTCTTTTG	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11639_Cjap_Sim2018_COI	DRWA-091	1	BE11639_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11639_Esin_And2018_Cyb	DRWA-091	1	BE11639_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-091	Cytb	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	ACCCCTCCTCATATCCAACCA	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11639_Mgig_Bot2012_COI	DRWA-091	1	BE11639_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	TCTTATTCGTTGGAGACTTTATAACCCT	130.87904
BE11639	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49148	WGS84	BE11639	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11639_Mjap_Gib2022_COI	DRWA-091	1	BE11639_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11639	Sample	BE11639	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-091	COI	45	BE11639	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-091	AATTTAATTGTGACTAGGCATGGTCTG	130.87904
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11641_Aamu_Bax2006_COI	DRWA-093	1	BE11641_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	GCACAACCGGGATCTTTACTTC	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11641_Pcan_Bot2011_IGS	DRWA-093	1	BE11641_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-093	IGS	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	CGTAATCCTCAGTACTGGCTA	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11641_Pper_Dia2013_COI	DRWA-093	1	BE11641_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	CTTAGTGGCATTAATTCGDAATCC	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11641_Sspa_Oph2007_COI	DRWA-093	1	BE11641_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	GAAGGCCTTCCGATGATC	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11641_Upin_Bot2011_COI	DRWA-093	1	BE11641_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	CTTTAATTACAGCGTTTTTATTGTTGT	130.87532
BE11641	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.49017	WGS84	BE11641	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11641_Vgib_Wil2025_COI	DRWA-093	1	BE11641_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11641	Sample	BE11641	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-093	COI	45	BE11641	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-093	CCCCTCTTTACAAGTTCTTTAGGGT	130.87532
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11642_Asen_Wil2025_COI	DRWA-094	1	BE11642_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	GTTGTTGTTACAACACATGCTCTTTTG	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11642_Cjap_Sim2018_COI	DRWA-094	1	BE11642_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11642_Esin_And2018_Cyb	DRWA-094	1	BE11642_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-094	Cytb	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	ACCCCTCCTCATATCCAACCA	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11642_Mgig_Bot2012_COI	DRWA-094	1	BE11642_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	TCTTATTCGTTGGAGACTTTATAACCCT	130.8769
BE11642	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.4902	WGS84	BE11642	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11642_Mjap_Gib2022_COI	DRWA-094	1	BE11642_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11642	Sample	BE11642	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-094	COI	45	BE11642	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-094	AATTTAATTGTGACTAGGCATGGTCTG	130.8769
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11643_Cmae_Bot2011_COI	DRWA-095	1	BE11643_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	ATGAACAGTCTATCCTCCTTTAG	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11643_Hsan_Wil2021_COI	DRWA-095	1	BE11643_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	CCTGGGCCGGTATAGTAGGT	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11643_Mros_Gib2022_COI	DRWA-095	1	BE11643_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11643_Msal_Bot2012_COI	DRWA-095	1	BE11643_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	GYTAGTTCCRATGATGTTAGCTG	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11643_Mstr_Wil2021_COI	DRWA-095	1	BE11643_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	GTAGTGGTAACTACTCATGCTTTA	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11643_Pvir_Dia2013_COI	DRWA-095	1	BE11643_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	CTTAGTGGCATTAATTCGDAATCC	130.87313
BE11643	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48876	WGS84	BE11643	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11643_Rhar_Sim2018_COI	DRWA-095	1	BE11643_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11643	Sample	BE11643	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-095	COI	45	BE11643	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-095	CTGCTGCTATTGCTCATG	130.87313
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11645_Aamu_Bax2006_COI	DRWA-097	1	BE11645_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	GCACAACCGGGATCTTTACTTC	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11645_Pcan_Bot2011_IGS	DRWA-097	1	BE11645_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-097	IGS	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	CGTAATCCTCAGTACTGGCTA	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11645_Pper_Dia2013_COI	DRWA-097	1	BE11645_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	CTTAGTGGCATTAATTCGDAATCC	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11645_Sspa_Oph2007_COI	DRWA-097	1	BE11645_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	GAAGGCCTTCCGATGATC	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11645_Upin_Bot2011_COI	DRWA-097	1	BE11645_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	CTTTAATTACAGCGTTTTTATTGTTGT	130.87592
BE11645	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48882	WGS84	BE11645	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11645_Vgib_Wil2025_COI	DRWA-097	1	BE11645_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11645	Sample	BE11645	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-097	COI	45	BE11645	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-097	CCCCTCTTTACAAGTTCTTTAGGGT	130.87592
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11646_Asen_Wil2025_COI	DRWA-098	1	BE11646_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	GTTGTTGTTACAACACATGCTCTTTTG	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11646_Cjap_Sim2018_COI	DRWA-098	1	BE11646_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11646_Esin_And2018_Cyb	DRWA-098	1	BE11646_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-098	Cytb	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	ACCCCTCCTCATATCCAACCA	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11646_Mgig_Bot2012_COI	DRWA-098	1	BE11646_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	TCTTATTCGTTGGAGACTTTATAACCCT	130.87388
BE11646	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48757	WGS84	BE11646	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11646_Mjap_Gib2022_COI	DRWA-098	1	BE11646_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11646	Sample	BE11646	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-098	COI	45	BE11646	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-098	AATTTAATTGTGACTAGGCATGGTCTG	130.87388
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11647_Cmae_Bot2011_COI	DRWA-099	1	BE11647_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	ATGAACAGTCTATCCTCCTTTAG	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11647_Hsan_Wil2021_COI	DRWA-099	1	BE11647_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	CCTGGGCCGGTATAGTAGGT	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11647_Mros_Gib2022_COI	DRWA-099	1	BE11647_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11647_Msal_Bot2012_COI	DRWA-099	1	BE11647_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	GYTAGTTCCRATGATGTTAGCTG	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11647_Mstr_Wil2021_COI	DRWA-099	1	BE11647_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	GTAGTGGTAACTACTCATGCTTTA	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11647_Pvir_Dia2013_COI	DRWA-099	1	BE11647_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	CTTAGTGGCATTAATTCGDAATCC	130.87343
BE11647	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48636	WGS84	BE11647	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11647_Rhar_Sim2018_COI	DRWA-099	1	BE11647_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11647	Sample	BE11647	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-099	COI	45	BE11647	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-099	CTGCTGCTATTGCTCATG	130.87343
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11649_Aamu_Bax2006_COI	DRWA-101	1	BE11649_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	GCACAACCGGGATCTTTACTTC	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11649_Pcan_Bot2011_IGS	DRWA-101	1	BE11649_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-101	IGS	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	CGTAATCCTCAGTACTGGCTA	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11649_Pper_Dia2013_COI	DRWA-101	1	BE11649_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	CTTAGTGGCATTAATTCGDAATCC	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11649_Sspa_Oph2007_COI	DRWA-101	1	BE11649_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	GAAGGCCTTCCGATGATC	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11649_Upin_Bot2011_COI	DRWA-101	1	BE11649_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	CTTTAATTACAGCGTTTTTATTGTTGT	130.87622
BE11649	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48646	WGS84	BE11649	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11649_Vgib_Wil2025_COI	DRWA-101	1	BE11649_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11649	Sample	BE11649	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-101	COI	45	BE11649	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-101	CCCCTCTTTACAAGTTCTTTAGGGT	130.87622
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11650_Cmae_Bot2011_COI	DRWA-102	1	BE11650_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	ATGAACAGTCTATCCTCCTTTAG	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11650_Hsan_Wil2021_COI	DRWA-102	1	BE11650_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	CCTGGGCCGGTATAGTAGGT	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11650_Mros_Gib2022_COI	DRWA-102	1	BE11650_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Mros_Gib2022_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11650_Msal_Bot2012_COI	DRWA-102	1	BE11650_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Msal_Bot2012_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	GYTAGTTCCRATGATGTTAGCTG	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11650_Mstr_Wil2021_COI	DRWA-102	1	BE11650_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	GTAGTGGTAACTACTCATGCTTTA	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11650_Pvir_Dia2013_COI	DRWA-102	1	BE11650_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	CTTAGTGGCATTAATTCGDAATCC	130.87416
BE11650	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48528	WGS84	BE11650	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11650_Rhar_Sim2018_COI	DRWA-102	1	BE11650_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11650	Sample	BE11650	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWA-102	COI	45	BE11650	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-102	CTGCTGCTATTGCTCATG	130.87416
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11652_Aamu_Bax2006_COI	DRWA-104	1	BE11652_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	GCACAACCGGGATCTTTACTTC	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11652_Pcan_Bot2011_IGS	DRWA-104	1	BE11652_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWA-104	IGS	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	CGTAATCCTCAGTACTGGCTA	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11652_Pper_Dia2013_COI	DRWA-104	1	BE11652_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Pper_Dia2013_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	CTTAGTGGCATTAATTCGDAATCC	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11652_Sspa_Oph2007_COI	DRWA-104	1	BE11652_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	GAAGGCCTTCCGATGATC	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11652_Upin_Bot2011_COI	DRWA-104	1	BE11652_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Upin_Bot2011_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	CTTTAATTACAGCGTTTTTATTGTTGT	130.87494
BE11652	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48408	WGS84	BE11652	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11652_Vgib_Wil2025_COI	DRWA-104	1	BE11652_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11652	Sample	BE11652	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWA-104	COI	45	BE11652	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-104	CCCCTCTTTACAAGTTCTTTAGGGT	130.87494
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11653_Asen_Wil2025_COI	DRWA-105	1	BE11653_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Asen_Wil2025_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	GTTGTTGTTACAACACATGCTCTTTTG	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11653_Cjap_Sim2018_COI	DRWA-105	1	BE11653_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11653_Esin_And2018_Cyb	DRWA-105	1	BE11653_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Esin_And2018_Cyb	DRWA-105	Cytb	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	ACCCCTCCTCATATCCAACCA	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11653_Mgig_Bot2012_COI	DRWA-105	1	BE11653_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	TCTTATTCGTTGGAGACTTTATAACCCT	130.87621
BE11653	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16382	-12.48406	WGS84	BE11653	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_A	11/08/2023	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11653_Mjap_Gib2022_COI	DRWA-105	1	BE11653_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11653	Sample	BE11653	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWA-105	COI	45	BE11653	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWA-105	AATTTAATTGTGACTAGGCATGGTCTG	130.87621
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11797_Cmae_Bot2011_COI	DRWB-100	1	BE11797_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	ATGAACAGTCTATCCTCCTTTAG	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11797_Hsan_Wil2021_COI	DRWB-100	1	BE11797_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	CCTGGGCCGGTATAGTAGGT	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11797_Mros_Gib2022_COI	DRWB-100	1	BE11797_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11797_Msal_Bot2012_COI	DRWB-100	1	BE11797_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	GYTAGTTCCRATGATGTTAGCTG	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11797_Mstr_Wil2021_COI	DRWB-100	1	BE11797_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	GTAGTGGTAACTACTCATGCTTTA	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11797_Pvir_Dia2013_COI	DRWB-100	1	BE11797_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	CTTAGTGGCATTAATTCGDAATCC	130.92721
BE11797	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.5077	WGS84	BE11797	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11797_Rhar_Sim2018_COI	DRWB-100	1	BE11797_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11797	Sample	BE11797	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-100	COI	45	BE11797	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-100	CTGCTGCTATTGCTCATG	130.92721
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11799_Aamu_Bax2006_COI	DRWB-102	1	BE11799_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	GCACAACCGGGATCTTTACTTC	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11799_Pcan_Bot2011_IGS	DRWB-102	1	BE11799_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-102	IGS	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	CGTAATCCTCAGTACTGGCTA	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11799_Pper_Dia2013_COI	DRWB-102	1	BE11799_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	CTTAGTGGCATTAATTCGDAATCC	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11799_Sspa_Oph2007_COI	DRWB-102	1	BE11799_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	GAAGGCCTTCCGATGATC	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11799_Upin_Bot2011_COI	DRWB-102	1	BE11799_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	CTTTAATTACAGCGTTTTTATTGTTGT	130.9292
BE11799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50785	WGS84	BE11799	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11799_Vgib_Wil2025_COI	DRWB-102	1	BE11799_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11799	Sample	BE11799	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-102	COI	45	BE11799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-102	CCCCTCTTTACAAGTTCTTTAGGGT	130.9292
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11800_Asen_Wil2025_COI	DRWB-103	1	BE11800_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	GTTGTTGTTACAACACATGCTCTTTTG	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11800_Cjap_Sim2018_COI	DRWB-103	1	BE11800_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11800_Esin_And2018_Cyb	DRWB-103	1	BE11800_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-103	Cytb	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	ACCCCTCCTCATATCCAACCA	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11800_Mgig_Bot2012_COI	DRWB-103	1	BE11800_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	TCTTATTCGTTGGAGACTTTATAACCCT	130.93014
BE11800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50773	WGS84	BE11800	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11800_Mjap_Gib2022_COI	DRWB-103	1	BE11800_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11800	Sample	BE11800	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-103	COI	45	BE11800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-103	AATTTAATTGTGACTAGGCATGGTCTG	130.93014
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11801_Cmae_Bot2011_COI	DRWB-104	1	BE11801_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	ATGAACAGTCTATCCTCCTTTAG	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11801_Hsan_Wil2021_COI	DRWB-104	1	BE11801_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	CCTGGGCCGGTATAGTAGGT	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11801_Mros_Gib2022_COI	DRWB-104	1	BE11801_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11801_Msal_Bot2012_COI	DRWB-104	1	BE11801_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	GYTAGTTCCRATGATGTTAGCTG	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11801_Mstr_Wil2021_COI	DRWB-104	1	BE11801_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	GTAGTGGTAACTACTCATGCTTTA	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11801_Pvir_Dia2013_COI	DRWB-104	1	BE11801_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	CTTAGTGGCATTAATTCGDAATCC	130.92768
BE11801	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50705	WGS84	BE11801	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11801_Rhar_Sim2018_COI	DRWB-104	1	BE11801_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11801	Sample	BE11801	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-104	COI	45	BE11801	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-104	CTGCTGCTATTGCTCATG	130.92768
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11803_Aamu_Bax2006_COI	DRWB-106	1	BE11803_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	GCACAACCGGGATCTTTACTTC	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11803_Pcan_Bot2011_IGS	DRWB-106	1	BE11803_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-106	IGS	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	CGTAATCCTCAGTACTGGCTA	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11803_Pper_Dia2013_COI	DRWB-106	1	BE11803_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	CTTAGTGGCATTAATTCGDAATCC	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11803_Sspa_Oph2007_COI	DRWB-106	1	BE11803_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	GAAGGCCTTCCGATGATC	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11803_Upin_Bot2011_COI	DRWB-106	1	BE11803_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	CTTTAATTACAGCGTTTTTATTGTTGT	130.88186
BE11803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49631	WGS84	BE11803	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11803_Vgib_Wil2025_COI	DRWB-106	1	BE11803_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11803	Sample	BE11803	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-106	COI	45	BE11803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-106	CCCCTCTTTACAAGTTCTTTAGGGT	130.88186
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11804_Asen_Wil2025_COI	DRWB-107	1	BE11804_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	GTTGTTGTTACAACACATGCTCTTTTG	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11804_Cjap_Sim2018_COI	DRWB-107	1	BE11804_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11804_Esin_And2018_Cyb	DRWB-107	1	BE11804_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-107	Cytb	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	ACCCCTCCTCATATCCAACCA	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11804_Mgig_Bot2012_COI	DRWB-107	1	BE11804_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	TCTTATTCGTTGGAGACTTTATAACCCT	130.88878
BE11804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4965	WGS84	BE11804	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11804_Mjap_Gib2022_COI	DRWB-107	1	BE11804_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11804	Sample	BE11804	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-107	COI	45	BE11804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-107	AATTTAATTGTGACTAGGCATGGTCTG	130.88878
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11805_Cmae_Bot2011_COI	DRWB-108	1	BE11805_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	ATGAACAGTCTATCCTCCTTTAG	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11805_Hsan_Wil2021_COI	DRWB-108	1	BE11805_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	CCTGGGCCGGTATAGTAGGT	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11805_Mros_Gib2022_COI	DRWB-108	1	BE11805_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11805_Msal_Bot2012_COI	DRWB-108	1	BE11805_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	GYTAGTTCCRATGATGTTAGCTG	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11805_Mstr_Wil2021_COI	DRWB-108	1	BE11805_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	GTAGTGGTAACTACTCATGCTTTA	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11805_Pvir_Dia2013_COI	DRWB-108	1	BE11805_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	CTTAGTGGCATTAATTCGDAATCC	130.89031
BE11805	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49653	WGS84	BE11805	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11805_Rhar_Sim2018_COI	DRWB-108	1	BE11805_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11805	Sample	BE11805	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-108	COI	45	BE11805	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-108	CTGCTGCTATTGCTCATG	130.89031
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11807_Aamu_Bax2006_COI	DRWB-110	1	BE11807_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	GCACAACCGGGATCTTTACTTC	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11807_Pcan_Bot2011_IGS	DRWB-110	1	BE11807_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-110	IGS	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	CGTAATCCTCAGTACTGGCTA	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11807_Pper_Dia2013_COI	DRWB-110	1	BE11807_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	CTTAGTGGCATTAATTCGDAATCC	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11807_Sspa_Oph2007_COI	DRWB-110	1	BE11807_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	GAAGGCCTTCCGATGATC	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11807_Upin_Bot2011_COI	DRWB-110	1	BE11807_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	CTTTAATTACAGCGTTTTTATTGTTGT	130.87946
BE11807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49495	WGS84	BE11807	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11807_Vgib_Wil2025_COI	DRWB-110	1	BE11807_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11807	Sample	BE11807	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-110	COI	45	BE11807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-110	CCCCTCTTTACAAGTTCTTTAGGGT	130.87946
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11808_Asen_Wil2025_COI	DRWB-111	1	BE11808_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	GTTGTTGTTACAACACATGCTCTTTTG	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11808_Cjap_Sim2018_COI	DRWB-111	1	BE11808_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11808_Esin_And2018_Cyb	DRWB-111	1	BE11808_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-111	Cytb	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	ACCCCTCCTCATATCCAACCA	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11808_Mgig_Bot2012_COI	DRWB-111	1	BE11808_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	TCTTATTCGTTGGAGACTTTATAACCCT	130.88104
BE11808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49504	WGS84	BE11808	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11808_Mjap_Gib2022_COI	DRWB-111	1	BE11808_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11808	Sample	BE11808	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-111	COI	45	BE11808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-111	AATTTAATTGTGACTAGGCATGGTCTG	130.88104
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11809_Cmae_Bot2011_COI	DRWB-112	1	BE11809_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	ATGAACAGTCTATCCTCCTTTAG	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11809_Hsan_Wil2021_COI	DRWB-112	1	BE11809_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	CCTGGGCCGGTATAGTAGGT	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11809_Mros_Gib2022_COI	DRWB-112	1	BE11809_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11809_Msal_Bot2012_COI	DRWB-112	1	BE11809_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	GYTAGTTCCRATGATGTTAGCTG	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11809_Mstr_Wil2021_COI	DRWB-112	1	BE11809_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	GTAGTGGTAACTACTCATGCTTTA	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11809_Pvir_Dia2013_COI	DRWB-112	1	BE11809_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	CTTAGTGGCATTAATTCGDAATCC	130.88251
BE11809	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4951	WGS84	BE11809	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11809_Rhar_Sim2018_COI	DRWB-112	1	BE11809_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11809	Sample	BE11809	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-112	COI	45	BE11809	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-112	CTGCTGCTATTGCTCATG	130.88251
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11810_Aamu_Bax2006_COI	DRWB-113	1	BE11810_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	GCACAACCGGGATCTTTACTTC	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11810_Pcan_Bot2011_IGS	DRWB-113	1	BE11810_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-113	IGS	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	CGTAATCCTCAGTACTGGCTA	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11810_Pper_Dia2013_COI	DRWB-113	1	BE11810_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	CTTAGTGGCATTAATTCGDAATCC	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11810_Sspa_Oph2007_COI	DRWB-113	1	BE11810_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	GAAGGCCTTCCGATGATC	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11810_Upin_Bot2011_COI	DRWB-113	1	BE11810_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	CTTTAATTACAGCGTTTTTATTGTTGT	130.88405
BE11810	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49513	WGS84	BE11810	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11810_Vgib_Wil2025_COI	DRWB-113	1	BE11810_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11810	Sample	BE11810	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-113	COI	45	BE11810	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-113	CCCCTCTTTACAAGTTCTTTAGGGT	130.88405
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11811_Asen_Wil2025_COI	DRWB-114	1	BE11811_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	GTTGTTGTTACAACACATGCTCTTTTG	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11811_Cjap_Sim2018_COI	DRWB-114	1	BE11811_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11811_Esin_And2018_Cyb	DRWB-114	1	BE11811_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-114	Cytb	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	ACCCCTCCTCATATCCAACCA	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11811_Mgig_Bot2012_COI	DRWB-114	1	BE11811_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	TCTTATTCGTTGGAGACTTTATAACCCT	130.88842
BE11811	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4952	WGS84	BE11811	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11811_Mjap_Gib2022_COI	DRWB-114	1	BE11811_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11811	Sample	BE11811	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-114	COI	45	BE11811	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-114	AATTTAATTGTGACTAGGCATGGTCTG	130.88842
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11812_Cmae_Bot2011_COI	DRWB-115	1	BE11812_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	ATGAACAGTCTATCCTCCTTTAG	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11812_Hsan_Wil2021_COI	DRWB-115	1	BE11812_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	CCTGGGCCGGTATAGTAGGT	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11812_Mros_Gib2022_COI	DRWB-115	1	BE11812_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11812_Msal_Bot2012_COI	DRWB-115	1	BE11812_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	GYTAGTTCCRATGATGTTAGCTG	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11812_Mstr_Wil2021_COI	DRWB-115	1	BE11812_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	GTAGTGGTAACTACTCATGCTTTA	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11812_Pvir_Dia2013_COI	DRWB-115	1	BE11812_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	CTTAGTGGCATTAATTCGDAATCC	130.88975
BE11812	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49527	WGS84	BE11812	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11812_Rhar_Sim2018_COI	DRWB-115	1	BE11812_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11812	Sample	BE11812	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-115	COI	45	BE11812	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-115	CTGCTGCTATTGCTCATG	130.88975
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11814_Aamu_Bax2006_COI	DRWB-117	1	BE11814_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	GCACAACCGGGATCTTTACTTC	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11814_Pcan_Bot2011_IGS	DRWB-117	1	BE11814_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-117	IGS	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	CGTAATCCTCAGTACTGGCTA	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11814_Pper_Dia2013_COI	DRWB-117	1	BE11814_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	CTTAGTGGCATTAATTCGDAATCC	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11814_Sspa_Oph2007_COI	DRWB-117	1	BE11814_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	GAAGGCCTTCCGATGATC	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11814_Upin_Bot2011_COI	DRWB-117	1	BE11814_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	CTTTAATTACAGCGTTTTTATTGTTGT	130.88021
BE11814	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49373	WGS84	BE11814	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11814_Vgib_Wil2025_COI	DRWB-117	1	BE11814_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11814	Sample	BE11814	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-117	COI	45	BE11814	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-117	CCCCTCTTTACAAGTTCTTTAGGGT	130.88021
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11815_Asen_Wil2025_COI	DRWB-118	1	BE11815_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	GTTGTTGTTACAACACATGCTCTTTTG	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11815_Cjap_Sim2018_COI	DRWB-118	1	BE11815_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11815_Esin_And2018_Cyb	DRWB-118	1	BE11815_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-118	Cytb	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	ACCCCTCCTCATATCCAACCA	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11815_Mgig_Bot2012_COI	DRWB-118	1	BE11815_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	TCTTATTCGTTGGAGACTTTATAACCCT	130.88173
BE11815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49381	WGS84	BE11815	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11815_Mjap_Gib2022_COI	DRWB-118	1	BE11815_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11815	Sample	BE11815	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-118	COI	45	BE11815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-118	AATTTAATTGTGACTAGGCATGGTCTG	130.88173
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11816_Cmae_Bot2011_COI	DRWB-119	1	BE11816_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	ATGAACAGTCTATCCTCCTTTAG	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11816_Hsan_Wil2021_COI	DRWB-119	1	BE11816_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	CCTGGGCCGGTATAGTAGGT	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11816_Mros_Gib2022_COI	DRWB-119	1	BE11816_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11816_Msal_Bot2012_COI	DRWB-119	1	BE11816_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	GYTAGTTCCRATGATGTTAGCTG	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11816_Mstr_Wil2021_COI	DRWB-119	1	BE11816_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	GTAGTGGTAACTACTCATGCTTTA	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11816_Pvir_Dia2013_COI	DRWB-119	1	BE11816_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	CTTAGTGGCATTAATTCGDAATCC	130.87668
BE11816	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49247	WGS84	BE11816	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11816_Rhar_Sim2018_COI	DRWB-119	1	BE11816_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11816	Sample	BE11816	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-119	COI	45	BE11816	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-119	CTGCTGCTATTGCTCATG	130.87668
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11818_Aamu_Bax2006_COI	DRWB-121	1	BE11818_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	GCACAACCGGGATCTTTACTTC	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11818_Pcan_Bot2011_IGS	DRWB-121	1	BE11818_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-121	IGS	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	CGTAATCCTCAGTACTGGCTA	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11818_Pper_Dia2013_COI	DRWB-121	1	BE11818_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	CTTAGTGGCATTAATTCGDAATCC	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11818_Sspa_Oph2007_COI	DRWB-121	1	BE11818_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	GAAGGCCTTCCGATGATC	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11818_Upin_Bot2011_COI	DRWB-121	1	BE11818_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	CTTTAATTACAGCGTTTTTATTGTTGT	130.87964
BE11818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49261	WGS84	BE11818	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11818_Vgib_Wil2025_COI	DRWB-121	1	BE11818_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11818	Sample	BE11818	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-121	COI	45	BE11818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-121	CCCCTCTTTACAAGTTCTTTAGGGT	130.87964
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11819_Asen_Wil2025_COI	DRWB-122	1	BE11819_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	GTTGTTGTTACAACACATGCTCTTTTG	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11819_Cjap_Sim2018_COI	DRWB-122	1	BE11819_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11819_Esin_And2018_Cyb	DRWB-122	1	BE11819_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-122	Cytb	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	ACCCCTCCTCATATCCAACCA	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11819_Mgig_Bot2012_COI	DRWB-122	1	BE11819_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	TCTTATTCGTTGGAGACTTTATAACCCT	130.88122
BE11819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49262	WGS84	BE11819	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11819_Mjap_Gib2022_COI	DRWB-122	1	BE11819_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11819	Sample	BE11819	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-122	COI	45	BE11819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-122	AATTTAATTGTGACTAGGCATGGTCTG	130.88122
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11821_Aamu_Bax2006_COI	DRWB-124	1	BE11821_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	GCACAACCGGGATCTTTACTTC	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11821_Pcan_Bot2011_IGS	DRWB-124	1	BE11821_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-124	IGS	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	CGTAATCCTCAGTACTGGCTA	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11821_Pper_Dia2013_COI	DRWB-124	1	BE11821_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	CTTAGTGGCATTAATTCGDAATCC	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11821_Sspa_Oph2007_COI	DRWB-124	1	BE11821_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	GAAGGCCTTCCGATGATC	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11821_Upin_Bot2011_COI	DRWB-124	1	BE11821_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	CTTTAATTACAGCGTTTTTATTGTTGT	130.87608
BE11821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11821	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11821_Vgib_Wil2025_COI	DRWB-124	1	BE11821_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11821	Sample	BE11821	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-124	COI	45	BE11821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-124	CCCCTCTTTACAAGTTCTTTAGGGT	130.87608
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11822_Asen_Wil2025_COI	DRWB-125	1	BE11822_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	GTTGTTGTTACAACACATGCTCTTTTG	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11822_Cjap_Sim2018_COI	DRWB-125	1	BE11822_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11822_Esin_And2018_Cyb	DRWB-125	1	BE11822_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-125	Cytb	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	ACCCCTCCTCATATCCAACCA	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11822_Mgig_Bot2012_COI	DRWB-125	1	BE11822_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	TCTTATTCGTTGGAGACTTTATAACCCT	130.87747
BE11822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49127	WGS84	BE11822	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11822_Mjap_Gib2022_COI	DRWB-125	1	BE11822_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11822	Sample	BE11822	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-125	COI	45	BE11822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-125	AATTTAATTGTGACTAGGCATGGTCTG	130.87747
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11823_Cmae_Bot2011_COI	DRWB-126	1	BE11823_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	ATGAACAGTCTATCCTCCTTTAG	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11823_Hsan_Wil2021_COI	DRWB-126	1	BE11823_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	CCTGGGCCGGTATAGTAGGT	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11823_Mros_Gib2022_COI	DRWB-126	1	BE11823_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11823_Msal_Bot2012_COI	DRWB-126	1	BE11823_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	GYTAGTTCCRATGATGTTAGCTG	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11823_Mstr_Wil2021_COI	DRWB-126	1	BE11823_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	GTAGTGGTAACTACTCATGCTTTA	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11823_Pvir_Dia2013_COI	DRWB-126	1	BE11823_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	CTTAGTGGCATTAATTCGDAATCC	130.87892
BE11823	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49129	WGS84	BE11823	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11823_Rhar_Sim2018_COI	DRWB-126	1	BE11823_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11823	Sample	BE11823	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-126	COI	45	BE11823	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-126	CTGCTGCTATTGCTCATG	130.87892
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11825_Aamu_Bax2006_COI	DRWB-128	1	BE11825_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	GCACAACCGGGATCTTTACTTC	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11825_Pcan_Bot2011_IGS	DRWB-128	1	BE11825_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-128	IGS	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	CGTAATCCTCAGTACTGGCTA	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11825_Pper_Dia2013_COI	DRWB-128	1	BE11825_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	CTTAGTGGCATTAATTCGDAATCC	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11825_Sspa_Oph2007_COI	DRWB-128	1	BE11825_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	GAAGGCCTTCCGATGATC	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11825_Upin_Bot2011_COI	DRWB-128	1	BE11825_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	CTTTAATTACAGCGTTTTTATTGTTGT	130.87545
BE11825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49003	WGS84	BE11825	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11825_Vgib_Wil2025_COI	DRWB-128	1	BE11825_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11825	Sample	BE11825	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-128	COI	45	BE11825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-128	CCCCTCTTTACAAGTTCTTTAGGGT	130.87545
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11826_Asen_Wil2025_COI	DRWB-129	1	BE11826_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	GTTGTTGTTACAACACATGCTCTTTTG	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11826_Cjap_Sim2018_COI	DRWB-129	1	BE11826_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11826_Esin_And2018_Cyb	DRWB-129	1	BE11826_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-129	Cytb	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	ACCCCTCCTCATATCCAACCA	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11826_Mgig_Bot2012_COI	DRWB-129	1	BE11826_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	TCTTATTCGTTGGAGACTTTATAACCCT	130.87695
BE11826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.49004	WGS84	BE11826	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11826_Mjap_Gib2022_COI	DRWB-129	1	BE11826_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11826	Sample	BE11826	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-129	COI	45	BE11826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-129	AATTTAATTGTGACTAGGCATGGTCTG	130.87695
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11827_Cmae_Bot2011_COI	DRWB-130	1	BE11827_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	ATGAACAGTCTATCCTCCTTTAG	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11827_Hsan_Wil2021_COI	DRWB-130	1	BE11827_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	CCTGGGCCGGTATAGTAGGT	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11827_Mros_Gib2022_COI	DRWB-130	1	BE11827_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11827_Msal_Bot2012_COI	DRWB-130	1	BE11827_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	GYTAGTTCCRATGATGTTAGCTG	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11827_Mstr_Wil2021_COI	DRWB-130	1	BE11827_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	GTAGTGGTAACTACTCATGCTTTA	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11827_Pvir_Dia2013_COI	DRWB-130	1	BE11827_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	CTTAGTGGCATTAATTCGDAATCC	130.87335
BE11827	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48874	WGS84	BE11827	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11827_Rhar_Sim2018_COI	DRWB-130	1	BE11827_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11827	Sample	BE11827	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-130	COI	45	BE11827	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-130	CTGCTGCTATTGCTCATG	130.87335
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11829_Aamu_Bax2006_COI	DRWB-132	1	BE11829_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	GCACAACCGGGATCTTTACTTC	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11829_Pcan_Bot2011_IGS	DRWB-132	1	BE11829_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-132	IGS	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	CGTAATCCTCAGTACTGGCTA	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11829_Pper_Dia2013_COI	DRWB-132	1	BE11829_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	CTTAGTGGCATTAATTCGDAATCC	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11829_Sspa_Oph2007_COI	DRWB-132	1	BE11829_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	GAAGGCCTTCCGATGATC	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11829_Upin_Bot2011_COI	DRWB-132	1	BE11829_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	CTTTAATTACAGCGTTTTTATTGTTGT	130.87609
BE11829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48881	WGS84	BE11829	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11829_Vgib_Wil2025_COI	DRWB-132	1	BE11829_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11829	Sample	BE11829	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-132	COI	45	BE11829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-132	CCCCTCTTTACAAGTTCTTTAGGGT	130.87609
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11830_Cmae_Bot2011_COI	DRWB-133	1	BE11830_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	ATGAACAGTCTATCCTCCTTTAG	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11830_Hsan_Wil2021_COI	DRWB-133	1	BE11830_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	CCTGGGCCGGTATAGTAGGT	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11830_Mros_Gib2022_COI	DRWB-133	1	BE11830_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11830_Msal_Bot2012_COI	DRWB-133	1	BE11830_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	GYTAGTTCCRATGATGTTAGCTG	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11830_Mstr_Wil2021_COI	DRWB-133	1	BE11830_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	GTAGTGGTAACTACTCATGCTTTA	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11830_Pvir_Dia2013_COI	DRWB-133	1	BE11830_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	CTTAGTGGCATTAATTCGDAATCC	130.87392
BE11830	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.4874	WGS84	BE11830	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11830_Rhar_Sim2018_COI	DRWB-133	1	BE11830_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11830	Sample	BE11830	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-133	COI	45	BE11830	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-133	CTGCTGCTATTGCTCATG	130.87392
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11832_Aamu_Bax2006_COI	DRWB-135	1	BE11832_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	GCACAACCGGGATCTTTACTTC	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11832_Pcan_Bot2011_IGS	DRWB-135	1	BE11832_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-135	IGS	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	CGTAATCCTCAGTACTGGCTA	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11832_Pper_Dia2013_COI	DRWB-135	1	BE11832_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	CTTAGTGGCATTAATTCGDAATCC	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11832_Sspa_Oph2007_COI	DRWB-135	1	BE11832_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	GAAGGCCTTCCGATGATC	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11832_Upin_Bot2011_COI	DRWB-135	1	BE11832_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	CTTTAATTACAGCGTTTTTATTGTTGT	130.87487
BE11832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.48636	WGS84	BE11832	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW04_23-24_B	3/07/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11832_Vgib_Wil2025_COI	DRWB-135	1	BE11832_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11832	Sample	BE11832	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-135	COI	45	BE11832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-135	CCCCTCTTTACAAGTTCTTTAGGGT	130.87487
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11876_Aamu_Bax2006_COI	DRWB-071	1	BE11876_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	GCACAACCGGGATCTTTACTTC	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11876_Pcan_Bot2011_IGS	DRWB-071	1	BE11876_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-071	IGS	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	CGTAATCCTCAGTACTGGCTA	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11876_Pper_Dia2013_COI	DRWB-071	1	BE11876_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	CTTAGTGGCATTAATTCGDAATCC	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11876_Sspa_Oph2007_COI	DRWB-071	1	BE11876_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	GAAGGCCTTCCGATGATC	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11876_Upin_Bot2011_COI	DRWB-071	1	BE11876_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	CTTTAATTACAGCGTTTTTATTGTTGT	130.93133
BE11876	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51538	WGS84	BE11876	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11876_Vgib_Wil2025_COI	DRWB-071	1	BE11876_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11876	Sample	BE11876	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-071	COI	45	BE11876	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-071	CCCCTCTTTACAAGTTCTTTAGGGT	130.93133
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11877_Asen_Wil2025_COI	DRWB-072	1	BE11877_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	GTTGTTGTTACAACACATGCTCTTTTG	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11877_Cjap_Sim2018_COI	DRWB-072	1	BE11877_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11877_Esin_And2018_Cyb	DRWB-072	1	BE11877_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-072	Cytb	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	ACCCCTCCTCATATCCAACCA	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11877_Mgig_Bot2012_COI	DRWB-072	1	BE11877_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	TCTTATTCGTTGGAGACTTTATAACCCT	130.93003
BE11877	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51476	WGS84	BE11877	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11877_Mjap_Gib2022_COI	DRWB-072	1	BE11877_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11877	Sample	BE11877	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-072	COI	45	BE11877	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-072	AATTTAATTGTGACTAGGCATGGTCTG	130.93003
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11878_Cmae_Bot2011_COI	DRWB-073	1	BE11878_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	ATGAACAGTCTATCCTCCTTTAG	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11878_Hsan_Wil2021_COI	DRWB-073	1	BE11878_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	CCTGGGCCGGTATAGTAGGT	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11878_Mros_Gib2022_COI	DRWB-073	1	BE11878_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11878_Msal_Bot2012_COI	DRWB-073	1	BE11878_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	GYTAGTTCCRATGATGTTAGCTG	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11878_Mstr_Wil2021_COI	DRWB-073	1	BE11878_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	GTAGTGGTAACTACTCATGCTTTA	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11878_Pvir_Dia2013_COI	DRWB-073	1	BE11878_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	CTTAGTGGCATTAATTCGDAATCC	130.93104
BE11878	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51485	WGS84	BE11878	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11878_Rhar_Sim2018_COI	DRWB-073	1	BE11878_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11878	Sample	BE11878	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-073	COI	45	BE11878	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-073	CTGCTGCTATTGCTCATG	130.93104
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11880_Asen_Wil2025_COI	DRWB-075	1	BE11880_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	GTTGTTGTTACAACACATGCTCTTTTG	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11880_Cjap_Sim2018_COI	DRWB-075	1	BE11880_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11880_Esin_And2018_Cyb	DRWB-075	1	BE11880_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-075	Cytb	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	ACCCCTCCTCATATCCAACCA	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11880_Mgig_Bot2012_COI	DRWB-075	1	BE11880_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	TCTTATTCGTTGGAGACTTTATAACCCT	130.93056
BE11880	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51385	WGS84	BE11880	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11880_Mjap_Gib2022_COI	DRWB-075	1	BE11880_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11880	Sample	BE11880	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-075	COI	45	BE11880	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-075	AATTTAATTGTGACTAGGCATGGTCTG	130.93056
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11881_Cmae_Bot2011_COI	DRWB-076	1	BE11881_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	ATGAACAGTCTATCCTCCTTTAG	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11881_Hsan_Wil2021_COI	DRWB-076	1	BE11881_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	CCTGGGCCGGTATAGTAGGT	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11881_Mros_Gib2022_COI	DRWB-076	1	BE11881_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11881_Msal_Bot2012_COI	DRWB-076	1	BE11881_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	GYTAGTTCCRATGATGTTAGCTG	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11881_Mstr_Wil2021_COI	DRWB-076	1	BE11881_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	GTAGTGGTAACTACTCATGCTTTA	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11881_Pvir_Dia2013_COI	DRWB-076	1	BE11881_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	CTTAGTGGCATTAATTCGDAATCC	130.93156
BE11881	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51394	WGS84	BE11881	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11881_Rhar_Sim2018_COI	DRWB-076	1	BE11881_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11881	Sample	BE11881	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-076	COI	45	BE11881	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-076	CTGCTGCTATTGCTCATG	130.93156
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11883_Aamu_Bax2006_COI	DRWB-078	1	BE11883_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	GCACAACCGGGATCTTTACTTC	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11883_Pcan_Bot2011_IGS	DRWB-078	1	BE11883_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-078	IGS	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	CGTAATCCTCAGTACTGGCTA	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11883_Pper_Dia2013_COI	DRWB-078	1	BE11883_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	CTTAGTGGCATTAATTCGDAATCC	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11883_Sspa_Oph2007_COI	DRWB-078	1	BE11883_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	GAAGGCCTTCCGATGATC	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11883_Upin_Bot2011_COI	DRWB-078	1	BE11883_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	CTTTAATTACAGCGTTTTTATTGTTGT	130.9301
BE11883	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51297	WGS84	BE11883	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11883_Vgib_Wil2025_COI	DRWB-078	1	BE11883_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11883	Sample	BE11883	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-078	COI	45	BE11883	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-078	CCCCTCTTTACAAGTTCTTTAGGGT	130.9301
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11884_Asen_Wil2025_COI	DRWB-079	1	BE11884_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	GTTGTTGTTACAACACATGCTCTTTTG	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11884_Cjap_Sim2018_COI	DRWB-079	1	BE11884_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11884_Esin_And2018_Cyb	DRWB-079	1	BE11884_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-079	Cytb	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	ACCCCTCCTCATATCCAACCA	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11884_Mgig_Bot2012_COI	DRWB-079	1	BE11884_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	TCTTATTCGTTGGAGACTTTATAACCCT	130.93108
BE11884	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51306	WGS84	BE11884	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11884_Mjap_Gib2022_COI	DRWB-079	1	BE11884_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11884	Sample	BE11884	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-079	COI	45	BE11884	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-079	AATTTAATTGTGACTAGGCATGGTCTG	130.93108
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11885_Cmae_Bot2011_COI	DRWB-080	1	BE11885_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	ATGAACAGTCTATCCTCCTTTAG	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11885_Hsan_Wil2021_COI	DRWB-080	1	BE11885_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	CCTGGGCCGGTATAGTAGGT	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11885_Mros_Gib2022_COI	DRWB-080	1	BE11885_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11885_Msal_Bot2012_COI	DRWB-080	1	BE11885_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	GYTAGTTCCRATGATGTTAGCTG	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11885_Mstr_Wil2021_COI	DRWB-080	1	BE11885_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	GTAGTGGTAACTACTCATGCTTTA	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11885_Pvir_Dia2013_COI	DRWB-080	1	BE11885_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	CTTAGTGGCATTAATTCGDAATCC	130.93206
BE11885	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51291	WGS84	BE11885	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11885_Rhar_Sim2018_COI	DRWB-080	1	BE11885_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11885	Sample	BE11885	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-080	COI	45	BE11885	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-080	CTGCTGCTATTGCTCATG	130.93206
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11887_Aamu_Bax2006_COI	DRWB-082	1	BE11887_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	GCACAACCGGGATCTTTACTTC	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11887_Pcan_Bot2011_IGS	DRWB-082	1	BE11887_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-082	IGS	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	CGTAATCCTCAGTACTGGCTA	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11887_Pper_Dia2013_COI	DRWB-082	1	BE11887_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	CTTAGTGGCATTAATTCGDAATCC	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11887_Sspa_Oph2007_COI	DRWB-082	1	BE11887_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	GAAGGCCTTCCGATGATC	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11887_Upin_Bot2011_COI	DRWB-082	1	BE11887_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	CTTTAATTACAGCGTTTTTATTGTTGT	130.93061
BE11887	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51226	WGS84	BE11887	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11887_Vgib_Wil2025_COI	DRWB-082	1	BE11887_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11887	Sample	BE11887	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-082	COI	45	BE11887	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-082	CCCCTCTTTACAAGTTCTTTAGGGT	130.93061
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11888_Asen_Wil2025_COI	DRWB-083	1	BE11888_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	GTTGTTGTTACAACACATGCTCTTTTG	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11888_Cjap_Sim2018_COI	DRWB-083	1	BE11888_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11888_Esin_And2018_Cyb	DRWB-083	1	BE11888_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-083	Cytb	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	ACCCCTCCTCATATCCAACCA	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11888_Mgig_Bot2012_COI	DRWB-083	1	BE11888_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	TCTTATTCGTTGGAGACTTTATAACCCT	130.9316
BE11888	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51224	WGS84	BE11888	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11888_Mjap_Gib2022_COI	DRWB-083	1	BE11888_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11888	Sample	BE11888	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-083	COI	45	BE11888	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-083	AATTTAATTGTGACTAGGCATGGTCTG	130.9316
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11889_Cmae_Bot2011_COI	DRWB-084	1	BE11889_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	ATGAACAGTCTATCCTCCTTTAG	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11889_Hsan_Wil2021_COI	DRWB-084	1	BE11889_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	CCTGGGCCGGTATAGTAGGT	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11889_Mros_Gib2022_COI	DRWB-084	1	BE11889_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11889_Msal_Bot2012_COI	DRWB-084	1	BE11889_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	GYTAGTTCCRATGATGTTAGCTG	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11889_Mstr_Wil2021_COI	DRWB-084	1	BE11889_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	GTAGTGGTAACTACTCATGCTTTA	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11889_Pvir_Dia2013_COI	DRWB-084	1	BE11889_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	CTTAGTGGCATTAATTCGDAATCC	130.92909
BE11889	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51139	WGS84	BE11889	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11889_Rhar_Sim2018_COI	DRWB-084	1	BE11889_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11889	Sample	BE11889	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-084	COI	45	BE11889	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-084	CTGCTGCTATTGCTCATG	130.92909
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11890_Aamu_Bax2006_COI	DRWB-085	1	BE11890_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	GCACAACCGGGATCTTTACTTC	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11890_Pcan_Bot2011_IGS	DRWB-085	1	BE11890_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-085	IGS	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	CGTAATCCTCAGTACTGGCTA	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11890_Pper_Dia2013_COI	DRWB-085	1	BE11890_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	CTTAGTGGCATTAATTCGDAATCC	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11890_Sspa_Oph2007_COI	DRWB-085	1	BE11890_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	GAAGGCCTTCCGATGATC	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11890_Upin_Bot2011_COI	DRWB-085	1	BE11890_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	CTTTAATTACAGCGTTTTTATTGTTGT	130.93012
BE11890	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51134	WGS84	BE11890	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11890_Vgib_Wil2025_COI	DRWB-085	1	BE11890_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11890	Sample	BE11890	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-085	COI	45	BE11890	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-085	CCCCTCTTTACAAGTTCTTTAGGGT	130.93012
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11891_Asen_Wil2025_COI	DRWB-086	1	BE11891_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	GTTGTTGTTACAACACATGCTCTTTTG	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11891_Cjap_Sim2018_COI	DRWB-086	1	BE11891_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11891_Esin_And2018_Cyb	DRWB-086	1	BE11891_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-086	Cytb	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	ACCCCTCCTCATATCCAACCA	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11891_Mgig_Bot2012_COI	DRWB-086	1	BE11891_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	TCTTATTCGTTGGAGACTTTATAACCCT	130.93112
BE11891	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51136	WGS84	BE11891	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11891_Mjap_Gib2022_COI	DRWB-086	1	BE11891_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11891	Sample	BE11891	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-086	COI	45	BE11891	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-086	AATTTAATTGTGACTAGGCATGGTCTG	130.93112
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11892_Cmae_Bot2011_COI	DRWB-087	1	BE11892_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	ATGAACAGTCTATCCTCCTTTAG	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11892_Hsan_Wil2021_COI	DRWB-087	1	BE11892_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	CCTGGGCCGGTATAGTAGGT	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11892_Mros_Gib2022_COI	DRWB-087	1	BE11892_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11892_Msal_Bot2012_COI	DRWB-087	1	BE11892_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	GYTAGTTCCRATGATGTTAGCTG	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11892_Mstr_Wil2021_COI	DRWB-087	1	BE11892_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	GTAGTGGTAACTACTCATGCTTTA	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11892_Pvir_Dia2013_COI	DRWB-087	1	BE11892_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	CTTAGTGGCATTAATTCGDAATCC	130.93215
BE11892	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51141	WGS84	BE11892	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11892_Rhar_Sim2018_COI	DRWB-087	1	BE11892_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11892	Sample	BE11892	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-087	COI	45	BE11892	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-087	CTGCTGCTATTGCTCATG	130.93215
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11894_Aamu_Bax2006_COI	DRWB-089	1	BE11894_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	GCACAACCGGGATCTTTACTTC	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11894_Pcan_Bot2011_IGS	DRWB-089	1	BE11894_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-089	IGS	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	CGTAATCCTCAGTACTGGCTA	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11894_Pper_Dia2013_COI	DRWB-089	1	BE11894_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	CTTAGTGGCATTAATTCGDAATCC	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11894_Sspa_Oph2007_COI	DRWB-089	1	BE11894_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	GAAGGCCTTCCGATGATC	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11894_Upin_Bot2011_COI	DRWB-089	1	BE11894_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	CTTTAATTACAGCGTTTTTATTGTTGT	130.92963
BE11894	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51037	WGS84	BE11894	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11894_Vgib_Wil2025_COI	DRWB-089	1	BE11894_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11894	Sample	BE11894	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-089	COI	45	BE11894	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-089	CCCCTCTTTACAAGTTCTTTAGGGT	130.92963
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11895_Asen_Wil2025_COI	DRWB-090	1	BE11895_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	GTTGTTGTTACAACACATGCTCTTTTG	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11895_Cjap_Sim2018_COI	DRWB-090	1	BE11895_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11895_Esin_And2018_Cyb	DRWB-090	1	BE11895_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-090	Cytb	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	ACCCCTCCTCATATCCAACCA	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11895_Mgig_Bot2012_COI	DRWB-090	1	BE11895_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	TCTTATTCGTTGGAGACTTTATAACCCT	130.93065
BE11895	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51044	WGS84	BE11895	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11895_Mjap_Gib2022_COI	DRWB-090	1	BE11895_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11895	Sample	BE11895	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-090	COI	45	BE11895	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-090	AATTTAATTGTGACTAGGCATGGTCTG	130.93065
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11896_Cmae_Bot2011_COI	DRWB-091	1	BE11896_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	ATGAACAGTCTATCCTCCTTTAG	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11896_Hsan_Wil2021_COI	DRWB-091	1	BE11896_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	CCTGGGCCGGTATAGTAGGT	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11896_Mros_Gib2022_COI	DRWB-091	1	BE11896_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11896_Msal_Bot2012_COI	DRWB-091	1	BE11896_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	GYTAGTTCCRATGATGTTAGCTG	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11896_Mstr_Wil2021_COI	DRWB-091	1	BE11896_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	GTAGTGGTAACTACTCATGCTTTA	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11896_Pvir_Dia2013_COI	DRWB-091	1	BE11896_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	CTTAGTGGCATTAATTCGDAATCC	130.93164
BE11896	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.51053	WGS84	BE11896	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11896_Rhar_Sim2018_COI	DRWB-091	1	BE11896_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11896	Sample	BE11896	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-091	COI	45	BE11896	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-091	CTGCTGCTATTGCTCATG	130.93164
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11898_Aamu_Bax2006_COI	DRWB-093	1	BE11898_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	GCACAACCGGGATCTTTACTTC	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11898_Pcan_Bot2011_IGS	DRWB-093	1	BE11898_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-093	IGS	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	CGTAATCCTCAGTACTGGCTA	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11898_Pper_Dia2013_COI	DRWB-093	1	BE11898_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	CTTAGTGGCATTAATTCGDAATCC	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11898_Sspa_Oph2007_COI	DRWB-093	1	BE11898_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	GAAGGCCTTCCGATGATC	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11898_Upin_Bot2011_COI	DRWB-093	1	BE11898_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	CTTTAATTACAGCGTTTTTATTGTTGT	130.92915
BE11898	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11898	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11898_Vgib_Wil2025_COI	DRWB-093	1	BE11898_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11898	Sample	BE11898	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-093	COI	45	BE11898	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-093	CCCCTCTTTACAAGTTCTTTAGGGT	130.92915
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11899_Asen_Wil2025_COI	DRWB-094	1	BE11899_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	GTTGTTGTTACAACACATGCTCTTTTG	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11899_Cjap_Sim2018_COI	DRWB-094	1	BE11899_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11899_Esin_And2018_Cyb	DRWB-094	1	BE11899_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-094	Cytb	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	ACCCCTCCTCATATCCAACCA	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11899_Mgig_Bot2012_COI	DRWB-094	1	BE11899_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	TCTTATTCGTTGGAGACTTTATAACCCT	130.93013
BE11899	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50948	WGS84	BE11899	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11899_Mjap_Gib2022_COI	DRWB-094	1	BE11899_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11899	Sample	BE11899	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-094	COI	45	BE11899	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-094	AATTTAATTGTGACTAGGCATGGTCTG	130.93013
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11900_Cmae_Bot2011_COI	DRWB-095	1	BE11900_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	ATGAACAGTCTATCCTCCTTTAG	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11900_Hsan_Wil2021_COI	DRWB-095	1	BE11900_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	CCTGGGCCGGTATAGTAGGT	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11900_Mros_Gib2022_COI	DRWB-095	1	BE11900_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11900_Msal_Bot2012_COI	DRWB-095	1	BE11900_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	GYTAGTTCCRATGATGTTAGCTG	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11900_Mstr_Wil2021_COI	DRWB-095	1	BE11900_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	GTAGTGGTAACTACTCATGCTTTA	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11900_Pvir_Dia2013_COI	DRWB-095	1	BE11900_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	CTTAGTGGCATTAATTCGDAATCC	130.93115
BE11900	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50956	WGS84	BE11900	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11900_Rhar_Sim2018_COI	DRWB-095	1	BE11900_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11900	Sample	BE11900	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-095	COI	45	BE11900	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-095	CTGCTGCTATTGCTCATG	130.93115
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11902_Aamu_Bax2006_COI	DRWB-097	1	BE11902_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	GCACAACCGGGATCTTTACTTC	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11902_Pcan_Bot2011_IGS	DRWB-097	1	BE11902_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-097	IGS	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	CGTAATCCTCAGTACTGGCTA	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11902_Pper_Dia2013_COI	DRWB-097	1	BE11902_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	CTTAGTGGCATTAATTCGDAATCC	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11902_Sspa_Oph2007_COI	DRWB-097	1	BE11902_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	GAAGGCCTTCCGATGATC	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11902_Upin_Bot2011_COI	DRWB-097	1	BE11902_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	CTTTAATTACAGCGTTTTTATTGTTGT	130.92861
BE11902	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50865	WGS84	BE11902	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11902_Vgib_Wil2025_COI	DRWB-097	1	BE11902_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11902	Sample	BE11902	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-097	COI	45	BE11902	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-097	CCCCTCTTTACAAGTTCTTTAGGGT	130.92861
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11903_Asen_Wil2025_COI	DRWB-098	1	BE11903_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	GTTGTTGTTACAACACATGCTCTTTTG	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11903_Cjap_Sim2018_COI	DRWB-098	1	BE11903_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11903_Esin_And2018_Cyb	DRWB-098	1	BE11903_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-098	Cytb	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	ACCCCTCCTCATATCCAACCA	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11903_Mgig_Bot2012_COI	DRWB-098	1	BE11903_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	TCTTATTCGTTGGAGACTTTATAACCCT	130.92962
BE11903	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50864	WGS84	BE11903	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11903_Mjap_Gib2022_COI	DRWB-098	1	BE11903_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11903	Sample	BE11903	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-098	COI	45	BE11903	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-098	AATTTAATTGTGACTAGGCATGGTCTG	130.92962
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11904_Cmae_Bot2011_COI	DRWB-099	1	BE11904_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	ATGAACAGTCTATCCTCCTTTAG	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11904_Hsan_Wil2021_COI	DRWB-099	1	BE11904_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	CCTGGGCCGGTATAGTAGGT	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11904_Mros_Gib2022_COI	DRWB-099	1	BE11904_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11904_Msal_Bot2012_COI	DRWB-099	1	BE11904_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	GYTAGTTCCRATGATGTTAGCTG	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11904_Mstr_Wil2021_COI	DRWB-099	1	BE11904_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	GTAGTGGTAACTACTCATGCTTTA	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11904_Pvir_Dia2013_COI	DRWB-099	1	BE11904_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	CTTAGTGGCATTAATTCGDAATCC	130.93073
BE11904	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16441	-12.50882	WGS84	BE11904	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW03_23-24_B	3/06/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11904_Rhar_Sim2018_COI	DRWB-099	1	BE11904_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11904	Sample	BE11904	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-099	COI	45	BE11904	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-099	CTGCTGCTATTGCTCATG	130.93073
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11913_Aamu_Bax2006_COI	GLTB-006	1	BE11913_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	GCACAACCGGGATCTTTACTTC	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11913_Pcan_Bot2011_IGS	GLTB-006	1	BE11913_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-006	IGS	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	CGTAATCCTCAGTACTGGCTA	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11913_Pper_Dia2013_COI	GLTB-006	1	BE11913_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	CTTAGTGGCATTAATTCGDAATCC	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11913_Sspa_Oph2007_COI	GLTB-006	1	BE11913_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	GAAGGCCTTCCGATGATC	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11913_Upin_Bot2011_COI	GLTB-006	1	BE11913_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	CTTTAATTACAGCGTTTTTATTGTTGT	151.32344
BE11913	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85357	WGS84	BE11913	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11913_Vgib_Wil2025_COI	GLTB-006	1	BE11913_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11913	Sample	BE11913	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-006	COI	45	BE11913	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-006	CCCCTCTTTACAAGTTCTTTAGGGT	151.32344
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11914_Asen_Wil2025_COI	GLTB-018	1	BE11914_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	GTTGTTGTTACAACACATGCTCTTTTG	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11914_Cjap_Sim2018_COI	GLTB-018	1	BE11914_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11914_Esin_And2018_Cyb	GLTB-018	1	BE11914_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-018	Cytb	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	ACCCCTCCTCATATCCAACCA	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11914_Mgig_Bot2012_COI	GLTB-018	1	BE11914_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	TCTTATTCGTTGGAGACTTTATAACCCT	151.32254
BE11914	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85057	WGS84	BE11914	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11914_Mjap_Gib2022_COI	GLTB-018	1	BE11914_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11914	Sample	BE11914	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-018	COI	45	BE11914	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-018	AATTTAATTGTGACTAGGCATGGTCTG	151.32254
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11915_Cmae_Bot2011_COI	GLTB-012	1	BE11915_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	ATGAACAGTCTATCCTCCTTTAG	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11915_Hsan_Wil2021_COI	GLTB-012	1	BE11915_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	CCTGGGCCGGTATAGTAGGT	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11915_Mros_Gib2022_COI	GLTB-012	1	BE11915_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11915_Msal_Bot2012_COI	GLTB-012	1	BE11915_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	GYTAGTTCCRATGATGTTAGCTG	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11915_Mstr_Wil2021_COI	GLTB-012	1	BE11915_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	GTAGTGGTAACTACTCATGCTTTA	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11915_Pvir_Dia2013_COI	GLTB-012	1	BE11915_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	CTTAGTGGCATTAATTCGDAATCC	151.32407
BE11915	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85162	WGS84	BE11915	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11915_Rhar_Sim2018_COI	GLTB-012	1	BE11915_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11915	Sample	BE11915	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-012	COI	45	BE11915	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-012	CTGCTGCTATTGCTCATG	151.32407
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11917_Aamu_Bax2006_COI	GLTB-030	1	BE11917_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	GCACAACCGGGATCTTTACTTC	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11917_Pcan_Bot2011_IGS	GLTB-030	1	BE11917_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-030	IGS	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	CGTAATCCTCAGTACTGGCTA	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11917_Pper_Dia2013_COI	GLTB-030	1	BE11917_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	CTTAGTGGCATTAATTCGDAATCC	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11917_Sspa_Oph2007_COI	GLTB-030	1	BE11917_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	GAAGGCCTTCCGATGATC	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11917_Upin_Bot2011_COI	GLTB-030	1	BE11917_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	CTTTAATTACAGCGTTTTTATTGTTGT	151.31018
BE11917	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84907	WGS84	BE11917	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11917_Vgib_Wil2025_COI	GLTB-030	1	BE11917_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11917	Sample	BE11917	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-030	COI	45	BE11917	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-030	CCCCTCTTTACAAGTTCTTTAGGGT	151.31018
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11918_Asen_Wil2025_COI	GLTB-029	1	BE11918_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	GTTGTTGTTACAACACATGCTCTTTTG	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11918_Cjap_Sim2018_COI	GLTB-029	1	BE11918_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11918_Esin_And2018_Cyb	GLTB-029	1	BE11918_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-029	Cytb	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	ACCCCTCCTCATATCCAACCA	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11918_Mgig_Bot2012_COI	GLTB-029	1	BE11918_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	TCTTATTCGTTGGAGACTTTATAACCCT	151.30871
BE11918	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84965	WGS84	BE11918	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11918_Mjap_Gib2022_COI	GLTB-029	1	BE11918_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11918	Sample	BE11918	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-029	COI	45	BE11918	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-029	AATTTAATTGTGACTAGGCATGGTCTG	151.30871
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11919_Cmae_Bot2011_COI	GLTB-005	1	BE11919_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	ATGAACAGTCTATCCTCCTTTAG	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11919_Hsan_Wil2021_COI	GLTB-005	1	BE11919_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	CCTGGGCCGGTATAGTAGGT	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11919_Mros_Gib2022_COI	GLTB-005	1	BE11919_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11919_Msal_Bot2012_COI	GLTB-005	1	BE11919_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	GYTAGTTCCRATGATGTTAGCTG	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11919_Mstr_Wil2021_COI	GLTB-005	1	BE11919_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	GTAGTGGTAACTACTCATGCTTTA	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11919_Pvir_Dia2013_COI	GLTB-005	1	BE11919_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	CTTAGTGGCATTAATTCGDAATCC	151.32604
BE11919	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85428	WGS84	BE11919	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11919_Rhar_Sim2018_COI	GLTB-005	1	BE11919_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11919	Sample	BE11919	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-005	COI	45	BE11919	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-005	CTGCTGCTATTGCTCATG	151.32604
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11920_Aamu_Bax2006_COI	GLTB-004	1	BE11920_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	GCACAACCGGGATCTTTACTTC	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11920_Pcan_Bot2011_IGS	GLTB-004	1	BE11920_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-004	IGS	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	CGTAATCCTCAGTACTGGCTA	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11920_Pper_Dia2013_COI	GLTB-004	1	BE11920_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	CTTAGTGGCATTAATTCGDAATCC	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11920_Sspa_Oph2007_COI	GLTB-004	1	BE11920_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	GAAGGCCTTCCGATGATC	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11920_Upin_Bot2011_COI	GLTB-004	1	BE11920_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	CTTTAATTACAGCGTTTTTATTGTTGT	151.32434
BE11920	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85438	WGS84	BE11920	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11920_Vgib_Wil2025_COI	GLTB-004	1	BE11920_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11920	Sample	BE11920	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-004	COI	45	BE11920	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-004	CCCCTCTTTACAAGTTCTTTAGGGT	151.32434
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11921_Asen_Wil2025_COI	GLTB-010	1	BE11921_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	GTTGTTGTTACAACACATGCTCTTTTG	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11921_Cjap_Sim2018_COI	GLTB-010	1	BE11921_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11921_Esin_And2018_Cyb	GLTB-010	1	BE11921_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-010	Cytb	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	ACCCCTCCTCATATCCAACCA	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11921_Mgig_Bot2012_COI	GLTB-010	1	BE11921_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	TCTTATTCGTTGGAGACTTTATAACCCT	151.32044
BE11921	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85252	WGS84	BE11921	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11921_Mjap_Gib2022_COI	GLTB-010	1	BE11921_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11921	Sample	BE11921	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-010	COI	45	BE11921	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-010	AATTTAATTGTGACTAGGCATGGTCTG	151.32044
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11922_Cmae_Bot2011_COI	GLTB-011	1	BE11922_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	ATGAACAGTCTATCCTCCTTTAG	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11922_Hsan_Wil2021_COI	GLTB-011	1	BE11922_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	CCTGGGCCGGTATAGTAGGT	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11922_Mros_Gib2022_COI	GLTB-011	1	BE11922_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11922_Msal_Bot2012_COI	GLTB-011	1	BE11922_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	GYTAGTTCCRATGATGTTAGCTG	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11922_Mstr_Wil2021_COI	GLTB-011	1	BE11922_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	GTAGTGGTAACTACTCATGCTTTA	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11922_Pvir_Dia2013_COI	GLTB-011	1	BE11922_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	CTTAGTGGCATTAATTCGDAATCC	151.32207
BE11922	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85182	WGS84	BE11922	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11922_Rhar_Sim2018_COI	GLTB-011	1	BE11922_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11922	Sample	BE11922	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-011	COI	45	BE11922	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-011	CTGCTGCTATTGCTCATG	151.32207
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11924_Aamu_Bax2006_COI	GLTB-023	1	BE11924_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	GCACAACCGGGATCTTTACTTC	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11924_Pcan_Bot2011_IGS	GLTB-023	1	BE11924_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-023	IGS	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	CGTAATCCTCAGTACTGGCTA	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11924_Pper_Dia2013_COI	GLTB-023	1	BE11924_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	CTTAGTGGCATTAATTCGDAATCC	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11924_Sspa_Oph2007_COI	GLTB-023	1	BE11924_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	GAAGGCCTTCCGATGATC	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11924_Upin_Bot2011_COI	GLTB-023	1	BE11924_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	CTTTAATTACAGCGTTTTTATTGTTGT	151.31305
BE11924	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85037	WGS84	BE11924	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11924_Vgib_Wil2025_COI	GLTB-023	1	BE11924_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11924	Sample	BE11924	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-023	COI	45	BE11924	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-023	CCCCTCTTTACAAGTTCTTTAGGGT	151.31305
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11925_Asen_Wil2025_COI	GLTB-022	1	BE11925_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	GTTGTTGTTACAACACATGCTCTTTTG	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11925_Cjap_Sim2018_COI	GLTB-022	1	BE11925_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11925_Esin_And2018_Cyb	GLTB-022	1	BE11925_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-022	Cytb	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	ACCCCTCCTCATATCCAACCA	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11925_Mgig_Bot2012_COI	GLTB-022	1	BE11925_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	TCTTATTCGTTGGAGACTTTATAACCCT	151.31132
BE11925	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85053	WGS84	BE11925	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11925_Mjap_Gib2022_COI	GLTB-022	1	BE11925_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11925	Sample	BE11925	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-022	COI	45	BE11925	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-022	AATTTAATTGTGACTAGGCATGGTCTG	151.31132
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11926_Cmae_Bot2011_COI	GLTB-016	1	BE11926_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	ATGAACAGTCTATCCTCCTTTAG	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11926_Hsan_Wil2021_COI	GLTB-016	1	BE11926_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	CCTGGGCCGGTATAGTAGGT	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11926_Mros_Gib2022_COI	GLTB-016	1	BE11926_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11926_Msal_Bot2012_COI	GLTB-016	1	BE11926_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	GYTAGTTCCRATGATGTTAGCTG	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11926_Mstr_Wil2021_COI	GLTB-016	1	BE11926_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	GTAGTGGTAACTACTCATGCTTTA	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11926_Pvir_Dia2013_COI	GLTB-016	1	BE11926_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	CTTAGTGGCATTAATTCGDAATCC	151.31957
BE11926	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85123	WGS84	BE11926	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11926_Rhar_Sim2018_COI	GLTB-016	1	BE11926_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11926	Sample	BE11926	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-016	COI	45	BE11926	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-016	CTGCTGCTATTGCTCATG	151.31957
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11928_Aamu_Bax2006_COI	GLTB-028	1	BE11928_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	GCACAACCGGGATCTTTACTTC	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11928_Pcan_Bot2011_IGS	GLTB-028	1	BE11928_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-028	IGS	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	CGTAATCCTCAGTACTGGCTA	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11928_Pper_Dia2013_COI	GLTB-028	1	BE11928_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	CTTAGTGGCATTAATTCGDAATCC	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11928_Sspa_Oph2007_COI	GLTB-028	1	BE11928_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	GAAGGCCTTCCGATGATC	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11928_Upin_Bot2011_COI	GLTB-028	1	BE11928_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	CTTTAATTACAGCGTTTTTATTGTTGT	151.30658
BE11928	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84957	WGS84	BE11928	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.6)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11928_Vgib_Wil2025_COI	GLTB-028	1	BE11928_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11928	Sample	BE11928	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-028	COI	45	BE11928	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-028	CCCCTCTTTACAAGTTCTTTAGGGT	151.30658
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11929_Asen_Wil2025_COI	GLTB-009	1	BE11929_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	GTTGTTGTTACAACACATGCTCTTTTG	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11929_Cjap_Sim2018_COI	GLTB-009	1	BE11929_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11929_Esin_And2018_Cyb	GLTB-009	1	BE11929_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-009	Cytb	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	ACCCCTCCTCATATCCAACCA	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11929_Mgig_Bot2012_COI	GLTB-009	1	BE11929_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	TCTTATTCGTTGGAGACTTTATAACCCT	151.31877
BE11929	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8522	WGS84	BE11929	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11929_Mjap_Gib2022_COI	GLTB-009	1	BE11929_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11929	Sample	BE11929	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-009	COI	45	BE11929	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-009	AATTTAATTGTGACTAGGCATGGTCTG	151.31877
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11931_Aamu_Bax2006_COI	GLTB-027	1	BE11931_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	GCACAACCGGGATCTTTACTTC	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11931_Pcan_Bot2011_IGS	GLTB-027	1	BE11931_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-027	IGS	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	CGTAATCCTCAGTACTGGCTA	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11931_Pper_Dia2013_COI	GLTB-027	1	BE11931_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	CTTAGTGGCATTAATTCGDAATCC	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11931_Sspa_Oph2007_COI	GLTB-027	1	BE11931_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	GAAGGCCTTCCGATGATC	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11931_Upin_Bot2011_COI	GLTB-027	1	BE11931_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	CTTTAATTACAGCGTTTTTATTGTTGT	151.31963
BE11931	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84902	WGS84	BE11931	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11931_Vgib_Wil2025_COI	GLTB-027	1	BE11931_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11931	Sample	BE11931	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-027	COI	45	BE11931	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-027	CCCCTCTTTACAAGTTCTTTAGGGT	151.31963
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11932_Asen_Wil2025_COI	GLTB-021	1	BE11932_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	GTTGTTGTTACAACACATGCTCTTTTG	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11932_Cjap_Sim2018_COI	GLTB-021	1	BE11932_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11932_Esin_And2018_Cyb	GLTB-021	1	BE11932_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-021	Cytb	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	ACCCCTCCTCATATCCAACCA	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11932_Mgig_Bot2012_COI	GLTB-021	1	BE11932_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	TCTTATTCGTTGGAGACTTTATAACCCT	151.3098
BE11932	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85082	WGS84	BE11932	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11932_Mjap_Gib2022_COI	GLTB-021	1	BE11932_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11932	Sample	BE11932	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-021	COI	45	BE11932	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-021	AATTTAATTGTGACTAGGCATGGTCTG	151.3098
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11933_Cmae_Bot2011_COI	GLTB-014	1	BE11933_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	ATGAACAGTCTATCCTCCTTTAG	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11933_Hsan_Wil2021_COI	GLTB-014	1	BE11933_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	CCTGGGCCGGTATAGTAGGT	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11933_Mros_Gib2022_COI	GLTB-014	1	BE11933_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11933_Msal_Bot2012_COI	GLTB-014	1	BE11933_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	GYTAGTTCCRATGATGTTAGCTG	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11933_Mstr_Wil2021_COI	GLTB-014	1	BE11933_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	GTAGTGGTAACTACTCATGCTTTA	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11933_Pvir_Dia2013_COI	GLTB-014	1	BE11933_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	CTTAGTGGCATTAATTCGDAATCC	151.31603
BE11933	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85132	WGS84	BE11933	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11933_Rhar_Sim2018_COI	GLTB-014	1	BE11933_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11933	Sample	BE11933	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-014	COI	45	BE11933	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-014	CTGCTGCTATTGCTCATG	151.31603
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11935_Aamu_Bax2006_COI	GLTB-026	1	BE11935_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	GCACAACCGGGATCTTTACTTC	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11935_Pcan_Bot2011_IGS	GLTB-026	1	BE11935_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-026	IGS	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	CGTAATCCTCAGTACTGGCTA	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11935_Pper_Dia2013_COI	GLTB-026	1	BE11935_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	CTTAGTGGCATTAATTCGDAATCC	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11935_Sspa_Oph2007_COI	GLTB-026	1	BE11935_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	GAAGGCCTTCCGATGATC	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11935_Upin_Bot2011_COI	GLTB-026	1	BE11935_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	CTTTAATTACAGCGTTTTTATTGTTGT	151.31817
BE11935	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84955	WGS84	BE11935	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11935_Vgib_Wil2025_COI	GLTB-026	1	BE11935_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11935	Sample	BE11935	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-026	COI	45	BE11935	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-026	CCCCTCTTTACAAGTTCTTTAGGGT	151.31817
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11936_Asen_Wil2025_COI	GLTB-020	1	BE11936_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	GTTGTTGTTACAACACATGCTCTTTTG	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11936_Cjap_Sim2018_COI	GLTB-020	1	BE11936_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11936_Esin_And2018_Cyb	GLTB-020	1	BE11936_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-020	Cytb	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	ACCCCTCCTCATATCCAACCA	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11936_Mgig_Bot2012_COI	GLTB-020	1	BE11936_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	TCTTATTCGTTGGAGACTTTATAACCCT	151.30812
BE11936	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85092	WGS84	BE11936	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11936_Mjap_Gib2022_COI	GLTB-020	1	BE11936_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11936	Sample	BE11936	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-020	COI	45	BE11936	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-020	AATTTAATTGTGACTAGGCATGGTCTG	151.30812
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11937_Cmae_Bot2011_COI	GLTB-019	1	BE11937_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	ATGAACAGTCTATCCTCCTTTAG	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11937_Hsan_Wil2021_COI	GLTB-019	1	BE11937_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	CCTGGGCCGGTATAGTAGGT	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11937_Mros_Gib2022_COI	GLTB-019	1	BE11937_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11937_Msal_Bot2012_COI	GLTB-019	1	BE11937_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	GYTAGTTCCRATGATGTTAGCTG	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11937_Mstr_Wil2021_COI	GLTB-019	1	BE11937_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	GTAGTGGTAACTACTCATGCTTTA	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11937_Pvir_Dia2013_COI	GLTB-019	1	BE11937_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	CTTAGTGGCATTAATTCGDAATCC	151.30599
BE11937	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85128	WGS84	BE11937	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11937_Rhar_Sim2018_COI	GLTB-019	1	BE11937_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11937	Sample	BE11937	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-019	COI	45	BE11937	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-019	CTGCTGCTATTGCTCATG	151.30599
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11939_Aamu_Bax2006_COI	GLTB-007	1	BE11939_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	GCACAACCGGGATCTTTACTTC	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11939_Pcan_Bot2011_IGS	GLTB-007	1	BE11939_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-007	IGS	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	CGTAATCCTCAGTACTGGCTA	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11939_Pper_Dia2013_COI	GLTB-007	1	BE11939_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	CTTAGTGGCATTAATTCGDAATCC	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11939_Sspa_Oph2007_COI	GLTB-007	1	BE11939_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	GAAGGCCTTCCGATGATC	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11939_Upin_Bot2011_COI	GLTB-007	1	BE11939_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	CTTTAATTACAGCGTTTTTATTGTTGT	151.32538
BE11939	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85292	WGS84	BE11939	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11939_Vgib_Wil2025_COI	GLTB-007	1	BE11939_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11939	Sample	BE11939	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-007	COI	45	BE11939	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-007	CCCCTCTTTACAAGTTCTTTAGGGT	151.32538
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11940_Cmae_Bot2011_COI	GLTB-013	1	BE11940_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	ATGAACAGTCTATCCTCCTTTAG	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11940_Hsan_Wil2021_COI	GLTB-013	1	BE11940_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	CCTGGGCCGGTATAGTAGGT	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11940_Mros_Gib2022_COI	GLTB-013	1	BE11940_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11940_Msal_Bot2012_COI	GLTB-013	1	BE11940_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	GYTAGTTCCRATGATGTTAGCTG	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11940_Mstr_Wil2021_COI	GLTB-013	1	BE11940_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	GTAGTGGTAACTACTCATGCTTTA	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11940_Pvir_Dia2013_COI	GLTB-013	1	BE11940_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	CTTAGTGGCATTAATTCGDAATCC	151.30703
BE11940	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85224	WGS84	BE11940	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11940_Rhar_Sim2018_COI	GLTB-013	1	BE11940_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11940	Sample	BE11940	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-013	COI	45	BE11940	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-013	CTGCTGCTATTGCTCATG	151.30703
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11942_Aamu_Bax2006_COI	GLTB-002	1	BE11942_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	GCACAACCGGGATCTTTACTTC	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11942_Pcan_Bot2011_IGS	GLTB-002	1	BE11942_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-002	IGS	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	CGTAATCCTCAGTACTGGCTA	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11942_Pper_Dia2013_COI	GLTB-002	1	BE11942_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	CTTAGTGGCATTAATTCGDAATCC	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11942_Sspa_Oph2007_COI	GLTB-002	1	BE11942_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	GAAGGCCTTCCGATGATC	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11942_Upin_Bot2011_COI	GLTB-002	1	BE11942_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	CTTTAATTACAGCGTTTTTATTGTTGT	151.32348
BE11942	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.85591	WGS84	BE11942	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11942_Vgib_Wil2025_COI	GLTB-002	1	BE11942_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11942	Sample	BE11942	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-002	COI	45	BE11942	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-002	CCCCTCTTTACAAGTTCTTTAGGGT	151.32348
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11943_Asen_Wil2025_COI	GLTB-031	1	BE11943_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	GTTGTTGTTACAACACATGCTCTTTTG	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11943_Cjap_Sim2018_COI	GLTB-031	1	BE11943_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11943_Esin_And2018_Cyb	GLTB-031	1	BE11943_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-031	Cytb	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	ACCCCTCCTCATATCCAACCA	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11943_Mgig_Bot2012_COI	GLTB-031	1	BE11943_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	TCTTATTCGTTGGAGACTTTATAACCCT	151.31176
BE11943	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11943	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11943_Mjap_Gib2022_COI	GLTB-031	1	BE11943_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11943	Sample	BE11943	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-031	COI	45	BE11943	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-031	AATTTAATTGTGACTAGGCATGGTCTG	151.31176
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11944_Cmae_Bot2011_COI	GLTB-032	1	BE11944_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	ATGAACAGTCTATCCTCCTTTAG	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11944_Mros_Gib2022_COI	GLTB-032	1	BE11944_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11944_Msal_Bot2012_COI	GLTB-032	1	BE11944_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	GYTAGTTCCRATGATGTTAGCTG	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11944_Mstr_Wil2021_COI	GLTB-032	1	BE11944_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	GTAGTGGTAACTACTCATGCTTTA	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11944_Pvir_Dia2013_COI	GLTB-032	1	BE11944_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	CTTAGTGGCATTAATTCGDAATCC	151.31378
BE11944	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84893	WGS84	BE11944	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11944_Rhar_Sim2018_COI	GLTB-032	1	BE11944_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11944	Sample	BE11944	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-032	COI	45	BE11944	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-032	CTGCTGCTATTGCTCATG	151.31378
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11946_Aamu_Bax2006_COI	GLTB-034	1	BE11946_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	GCACAACCGGGATCTTTACTTC	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11946_Pcan_Bot2011_IGS	GLTB-034	1	BE11946_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-034	IGS	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	CGTAATCCTCAGTACTGGCTA	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11946_Pper_Dia2013_COI	GLTB-034	1	BE11946_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	CTTAGTGGCATTAATTCGDAATCC	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11946_Sspa_Oph2007_COI	GLTB-034	1	BE11946_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	GAAGGCCTTCCGATGATC	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11946_Upin_Bot2011_COI	GLTB-034	1	BE11946_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	CTTTAATTACAGCGTTTTTATTGTTGT	151.31117
BE11946	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84791	WGS84	BE11946	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.7)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11946_Vgib_Wil2025_COI	GLTB-034	1	BE11946_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11946	Sample	BE11946	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-034	COI	45	BE11946	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-034	CCCCTCTTTACAAGTTCTTTAGGGT	151.31117
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11947_Asen_Wil2025_COI	GLTB-035	1	BE11947_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	GTTGTTGTTACAACACATGCTCTTTTG	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11947_Cjap_Sim2018_COI	GLTB-035	1	BE11947_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11947_Esin_And2018_Cyb	GLTB-035	1	BE11947_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-035	Cytb	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	ACCCCTCCTCATATCCAACCA	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11947_Mgig_Bot2012_COI	GLTB-035	1	BE11947_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	TCTTATTCGTTGGAGACTTTATAACCCT	151.31271
BE11947	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.84746	WGS84	BE11947	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT01_23-24_B	15/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11947_Mjap_Gib2022_COI	GLTB-035	1	BE11947_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11947	Sample	BE11947	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-035	COI	45	BE11947	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-035	AATTTAATTGTGACTAGGCATGGTCTG	151.31271
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11953_Aamu_Bax2006_COI	GLTB-042	1	BE11953_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	GCACAACCGGGATCTTTACTTC	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11953_Pcan_Bot2011_IGS	GLTB-042	1	BE11953_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-042	IGS	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	CGTAATCCTCAGTACTGGCTA	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11953_Pper_Dia2013_COI	GLTB-042	1	BE11953_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	CTTAGTGGCATTAATTCGDAATCC	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11953_Sspa_Oph2007_COI	GLTB-042	1	BE11953_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	GAAGGCCTTCCGATGATC	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11953_Upin_Bot2011_COI	GLTB-042	1	BE11953_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	CTTTAATTACAGCGTTTTTATTGTTGT	151.2668
BE11953	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83407	WGS84	BE11953	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11953_Vgib_Wil2025_COI	GLTB-042	1	BE11953_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11953	Sample	BE11953	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-042	COI	45	BE11953	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-042	CCCCTCTTTACAAGTTCTTTAGGGT	151.2668
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11954_Asen_Wil2025_COI	GLTB-043	1	BE11954_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	GTTGTTGTTACAACACATGCTCTTTTG	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11954_Cjap_Sim2018_COI	GLTB-043	1	BE11954_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11954_Esin_And2018_Cyb	GLTB-043	1	BE11954_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-043	Cytb	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	ACCCCTCCTCATATCCAACCA	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11954_Mgig_Bot2012_COI	GLTB-043	1	BE11954_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	TCTTATTCGTTGGAGACTTTATAACCCT	151.26832
BE11954	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8337	WGS84	BE11954	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11954_Mjap_Gib2022_COI	GLTB-043	1	BE11954_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11954	Sample	BE11954	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-043	COI	45	BE11954	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-043	AATTTAATTGTGACTAGGCATGGTCTG	151.26832
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11955_Cmae_Bot2011_COI	GLTB-044	1	BE11955_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	ATGAACAGTCTATCCTCCTTTAG	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11955_Hsan_Wil2021_COI	GLTB-044	1	BE11955_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	CCTGGGCCGGTATAGTAGGT	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11955_Mros_Gib2022_COI	GLTB-044	1	BE11955_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11955_Msal_Bot2012_COI	GLTB-044	1	BE11955_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	GYTAGTTCCRATGATGTTAGCTG	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11955_Mstr_Wil2021_COI	GLTB-044	1	BE11955_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	GTAGTGGTAACTACTCATGCTTTA	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11955_Pvir_Dia2013_COI	GLTB-044	1	BE11955_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	CTTAGTGGCATTAATTCGDAATCC	151.27042
BE11955	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83343	WGS84	BE11955	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11955_Rhar_Sim2018_COI	GLTB-044	1	BE11955_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11955	Sample	BE11955	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-044	COI	45	BE11955	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-044	CTGCTGCTATTGCTCATG	151.27042
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11957_Aamu_Bax2006_COI	GLTB-046	1	BE11957_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	GCACAACCGGGATCTTTACTTC	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11957_Pcan_Bot2011_IGS	GLTB-046	1	BE11957_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-046	IGS	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	CGTAATCCTCAGTACTGGCTA	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11957_Pper_Dia2013_COI	GLTB-046	1	BE11957_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	CTTAGTGGCATTAATTCGDAATCC	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11957_Sspa_Oph2007_COI	GLTB-046	1	BE11957_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	GAAGGCCTTCCGATGATC	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11957_Upin_Bot2011_COI	GLTB-046	1	BE11957_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	CTTTAATTACAGCGTTTTTATTGTTGT	151.27382
BE11957	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83296	WGS84	BE11957	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11957_Vgib_Wil2025_COI	GLTB-046	1	BE11957_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11957	Sample	BE11957	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-046	COI	45	BE11957	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-046	CCCCTCTTTACAAGTTCTTTAGGGT	151.27382
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11958_Asen_Wil2025_COI	GLTB-053	1	BE11958_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	GTTGTTGTTACAACACATGCTCTTTTG	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11958_Cjap_Sim2018_COI	GLTB-053	1	BE11958_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11958_Esin_And2018_Cyb	GLTB-053	1	BE11958_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-053	Cytb	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	ACCCCTCCTCATATCCAACCA	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11958_Mgig_Bot2012_COI	GLTB-053	1	BE11958_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	TCTTATTCGTTGGAGACTTTATAACCCT	151.26226
BE11958	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83167	WGS84	BE11958	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11958_Mjap_Gib2022_COI	GLTB-053	1	BE11958_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11958	Sample	BE11958	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-053	COI	45	BE11958	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-053	AATTTAATTGTGACTAGGCATGGTCTG	151.26226
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11959_Cmae_Bot2011_COI	GLTB-047	1	BE11959_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	ATGAACAGTCTATCCTCCTTTAG	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11959_Hsan_Wil2021_COI	GLTB-047	1	BE11959_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	CCTGGGCCGGTATAGTAGGT	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11959_Mros_Gib2022_COI	GLTB-047	1	BE11959_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11959_Msal_Bot2012_COI	GLTB-047	1	BE11959_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	GYTAGTTCCRATGATGTTAGCTG	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11959_Mstr_Wil2021_COI	GLTB-047	1	BE11959_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	GTAGTGGTAACTACTCATGCTTTA	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11959_Pvir_Dia2013_COI	GLTB-047	1	BE11959_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	CTTAGTGGCATTAATTCGDAATCC	151.27558
BE11959	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8327	WGS84	BE11959	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11959_Rhar_Sim2018_COI	GLTB-047	1	BE11959_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11959	Sample	BE11959	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-047	COI	45	BE11959	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-047	CTGCTGCTATTGCTCATG	151.27558
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11960_Aamu_Bax2006_COI	GLTB-048	1	BE11960_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	GCACAACCGGGATCTTTACTTC	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11960_Pcan_Bot2011_IGS	GLTB-048	1	BE11960_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-048	IGS	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	CGTAATCCTCAGTACTGGCTA	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11960_Pper_Dia2013_COI	GLTB-048	1	BE11960_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	CTTAGTGGCATTAATTCGDAATCC	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11960_Sspa_Oph2007_COI	GLTB-048	1	BE11960_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	GAAGGCCTTCCGATGATC	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11960_Upin_Bot2011_COI	GLTB-048	1	BE11960_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	CTTTAATTACAGCGTTTTTATTGTTGT	151.26536
BE11960	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83283	WGS84	BE11960	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11960_Vgib_Wil2025_COI	GLTB-048	1	BE11960_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11960	Sample	BE11960	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-048	COI	45	BE11960	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-048	CCCCTCTTTACAAGTTCTTTAGGGT	151.26536
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11961_Asen_Wil2025_COI	GLTB-049	1	BE11961_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	GTTGTTGTTACAACACATGCTCTTTTG	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11961_Cjap_Sim2018_COI	GLTB-049	1	BE11961_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11961_Esin_And2018_Cyb	GLTB-049	1	BE11961_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-049	Cytb	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	ACCCCTCCTCATATCCAACCA	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11961_Mgig_Bot2012_COI	GLTB-049	1	BE11961_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	TCTTATTCGTTGGAGACTTTATAACCCT	151.26732
BE11961	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83245	WGS84	BE11961	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11961_Mjap_Gib2022_COI	GLTB-049	1	BE11961_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11961	Sample	BE11961	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-049	COI	45	BE11961	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-049	AATTTAATTGTGACTAGGCATGGTCTG	151.26732
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11962_Cmae_Bot2011_COI	GLTB-050	1	BE11962_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	ATGAACAGTCTATCCTCCTTTAG	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11962_Hsan_Wil2021_COI	GLTB-050	1	BE11962_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	CCTGGGCCGGTATAGTAGGT	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11962_Mros_Gib2022_COI	GLTB-050	1	BE11962_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11962_Msal_Bot2012_COI	GLTB-050	1	BE11962_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	GYTAGTTCCRATGATGTTAGCTG	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11962_Mstr_Wil2021_COI	GLTB-050	1	BE11962_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	GTAGTGGTAACTACTCATGCTTTA	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11962_Pvir_Dia2013_COI	GLTB-050	1	BE11962_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	CTTAGTGGCATTAATTCGDAATCC	151.26908
BE11962	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8323	WGS84	BE11962	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11962_Rhar_Sim2018_COI	GLTB-050	1	BE11962_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11962	Sample	BE11962	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-050	COI	45	BE11962	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-050	CTGCTGCTATTGCTCATG	151.26908
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11964_Aamu_Bax2006_COI	GLTB-052	1	BE11964_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	GCACAACCGGGATCTTTACTTC	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11964_Pcan_Bot2011_IGS	GLTB-052	1	BE11964_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-052	IGS	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	CGTAATCCTCAGTACTGGCTA	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11964_Pper_Dia2013_COI	GLTB-052	1	BE11964_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	CTTAGTGGCATTAATTCGDAATCC	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11964_Sspa_Oph2007_COI	GLTB-052	1	BE11964_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	GAAGGCCTTCCGATGATC	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11964_Upin_Bot2011_COI	GLTB-052	1	BE11964_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	CTTTAATTACAGCGTTTTTATTGTTGT	151.27254
BE11964	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83164	WGS84	BE11964	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11964_Vgib_Wil2025_COI	GLTB-052	1	BE11964_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11964	Sample	BE11964	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-052	COI	45	BE11964	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-052	CCCCTCTTTACAAGTTCTTTAGGGT	151.27254
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11965_Asen_Wil2025_COI	GLTB-054	1	BE11965_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	GTTGTTGTTACAACACATGCTCTTTTG	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11965_Cjap_Sim2018_COI	GLTB-054	1	BE11965_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11965_Esin_And2018_Cyb	GLTB-054	1	BE11965_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-054	Cytb	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	ACCCCTCCTCATATCCAACCA	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11965_Mgig_Bot2012_COI	GLTB-054	1	BE11965_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	TCTTATTCGTTGGAGACTTTATAACCCT	151.26444
BE11965	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83133	WGS84	BE11965	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11965_Mjap_Gib2022_COI	GLTB-054	1	BE11965_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11965	Sample	BE11965	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-054	COI	45	BE11965	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-054	AATTTAATTGTGACTAGGCATGGTCTG	151.26444
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11966_Cmae_Bot2011_COI	GLTB-055	1	BE11966_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	ATGAACAGTCTATCCTCCTTTAG	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11966_Hsan_Wil2021_COI	GLTB-055	1	BE11966_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	CCTGGGCCGGTATAGTAGGT	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11966_Mros_Gib2022_COI	GLTB-055	1	BE11966_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11966_Msal_Bot2012_COI	GLTB-055	1	BE11966_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	GYTAGTTCCRATGATGTTAGCTG	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11966_Mstr_Wil2021_COI	GLTB-055	1	BE11966_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	GTAGTGGTAACTACTCATGCTTTA	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11966_Pvir_Dia2013_COI	GLTB-055	1	BE11966_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	CTTAGTGGCATTAATTCGDAATCC	151.26628
BE11966	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83129	WGS84	BE11966	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11966_Rhar_Sim2018_COI	GLTB-055	1	BE11966_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11966	Sample	BE11966	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-055	COI	45	BE11966	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-055	CTGCTGCTATTGCTCATG	151.26628
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11968_Aamu_Bax2006_COI	GLTB-057	1	BE11968_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	GCACAACCGGGATCTTTACTTC	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11968_Pcan_Bot2011_IGS	GLTB-057	1	BE11968_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-057	IGS	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	CGTAATCCTCAGTACTGGCTA	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11968_Pper_Dia2013_COI	GLTB-057	1	BE11968_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	CTTAGTGGCATTAATTCGDAATCC	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11968_Sspa_Oph2007_COI	GLTB-057	1	BE11968_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	GAAGGCCTTCCGATGATC	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11968_Upin_Bot2011_COI	GLTB-057	1	BE11968_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	CTTTAATTACAGCGTTTTTATTGTTGT	151.25752
BE11968	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83087	WGS84	BE11968	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11968_Vgib_Wil2025_COI	GLTB-057	1	BE11968_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11968	Sample	BE11968	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-057	COI	45	BE11968	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-057	CCCCTCTTTACAAGTTCTTTAGGGT	151.25752
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11969_Asen_Wil2025_COI	GLTB-058	1	BE11969_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	GTTGTTGTTACAACACATGCTCTTTTG	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11969_Cjap_Sim2018_COI	GLTB-058	1	BE11969_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11969_Esin_And2018_Cyb	GLTB-058	1	BE11969_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-058	Cytb	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	ACCCCTCCTCATATCCAACCA	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11969_Mgig_Bot2012_COI	GLTB-058	1	BE11969_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	TCTTATTCGTTGGAGACTTTATAACCCT	151.25955
BE11969	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83074	WGS84	BE11969	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11969_Mjap_Gib2022_COI	GLTB-058	1	BE11969_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11969	Sample	BE11969	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-058	COI	45	BE11969	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-058	AATTTAATTGTGACTAGGCATGGTCTG	151.25955
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11971_Aamu_Bax2006_COI	GLTB-060	1	BE11971_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	GCACAACCGGGATCTTTACTTC	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11971_Pcan_Bot2011_IGS	GLTB-060	1	BE11971_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-060	IGS	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	CGTAATCCTCAGTACTGGCTA	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11971_Pper_Dia2013_COI	GLTB-060	1	BE11971_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	CTTAGTGGCATTAATTCGDAATCC	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11971_Sspa_Oph2007_COI	GLTB-060	1	BE11971_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	GAAGGCCTTCCGATGATC	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11971_Upin_Bot2011_COI	GLTB-060	1	BE11971_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	CTTTAATTACAGCGTTTTTATTGTTGT	151.26334
BE11971	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83024	WGS84	BE11971	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11971_Vgib_Wil2025_COI	GLTB-060	1	BE11971_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11971	Sample	BE11971	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-060	COI	45	BE11971	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-060	CCCCTCTTTACAAGTTCTTTAGGGT	151.26334
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11972_Asen_Wil2025_COI	GLTB-061	1	BE11972_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	GTTGTTGTTACAACACATGCTCTTTTG	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11972_Cjap_Sim2018_COI	GLTB-061	1	BE11972_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11972_Esin_And2018_Cyb	GLTB-061	1	BE11972_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-061	Cytb	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	ACCCCTCCTCATATCCAACCA	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11972_Mgig_Bot2012_COI	GLTB-061	1	BE11972_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	TCTTATTCGTTGGAGACTTTATAACCCT	151.26491
BE11972	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82981	WGS84	BE11972	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11972_Mjap_Gib2022_COI	GLTB-061	1	BE11972_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11972	Sample	BE11972	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-061	COI	45	BE11972	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-061	AATTTAATTGTGACTAGGCATGGTCTG	151.26491
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11973_Cmae_Bot2011_COI	GLTB-062	1	BE11973_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	ATGAACAGTCTATCCTCCTTTAG	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11973_Hsan_Wil2021_COI	GLTB-062	1	BE11973_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	CCTGGGCCGGTATAGTAGGT	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11973_Mros_Gib2022_COI	GLTB-062	1	BE11973_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11973_Msal_Bot2012_COI	GLTB-062	1	BE11973_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	GYTAGTTCCRATGATGTTAGCTG	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11973_Mstr_Wil2021_COI	GLTB-062	1	BE11973_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	GTAGTGGTAACTACTCATGCTTTA	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11973_Pvir_Dia2013_COI	GLTB-062	1	BE11973_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	CTTAGTGGCATTAATTCGDAATCC	151.25297
BE11973	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82995	WGS84	BE11973	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11973_Rhar_Sim2018_COI	GLTB-062	1	BE11973_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11973	Sample	BE11973	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-062	COI	45	BE11973	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-062	CTGCTGCTATTGCTCATG	151.25297
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11975_Aamu_Bax2006_COI	GLTB-064	1	BE11975_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	GCACAACCGGGATCTTTACTTC	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11975_Pcan_Bot2011_IGS	GLTB-064	1	BE11975_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-064	IGS	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	CGTAATCCTCAGTACTGGCTA	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11975_Pper_Dia2013_COI	GLTB-064	1	BE11975_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	CTTAGTGGCATTAATTCGDAATCC	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11975_Sspa_Oph2007_COI	GLTB-064	1	BE11975_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	GAAGGCCTTCCGATGATC	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11975_Upin_Bot2011_COI	GLTB-064	1	BE11975_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	CTTTAATTACAGCGTTTTTATTGTTGT	151.25657
BE11975	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82934	WGS84	BE11975	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11975_Vgib_Wil2025_COI	GLTB-064	1	BE11975_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11975	Sample	BE11975	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-064	COI	45	BE11975	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-064	CCCCTCTTTACAAGTTCTTTAGGGT	151.25657
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11976_Asen_Wil2025_COI	GLTB-065	1	BE11976_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	GTTGTTGTTACAACACATGCTCTTTTG	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11976_Cjap_Sim2018_COI	GLTB-065	1	BE11976_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11976_Esin_And2018_Cyb	GLTB-065	1	BE11976_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-065	Cytb	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	ACCCCTCCTCATATCCAACCA	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11976_Mgig_Bot2012_COI	GLTB-065	1	BE11976_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	TCTTATTCGTTGGAGACTTTATAACCCT	151.25842
BE11976	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82913	WGS84	BE11976	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11976_Mjap_Gib2022_COI	GLTB-065	1	BE11976_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11976	Sample	BE11976	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-065	COI	45	BE11976	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-065	AATTTAATTGTGACTAGGCATGGTCTG	151.25842
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11977_Cmae_Bot2011_COI	GLTB-066	1	BE11977_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	ATGAACAGTCTATCCTCCTTTAG	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11977_Hsan_Wil2021_COI	GLTB-066	1	BE11977_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	CCTGGGCCGGTATAGTAGGT	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11977_Mros_Gib2022_COI	GLTB-066	1	BE11977_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11977_Msal_Bot2012_COI	GLTB-066	1	BE11977_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	GYTAGTTCCRATGATGTTAGCTG	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11977_Mstr_Wil2021_COI	GLTB-066	1	BE11977_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	GTAGTGGTAACTACTCATGCTTTA	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11977_Pvir_Dia2013_COI	GLTB-066	1	BE11977_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	CTTAGTGGCATTAATTCGDAATCC	151.2599
BE11977	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.8285	WGS84	BE11977	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11977_Rhar_Sim2018_COI	GLTB-066	1	BE11977_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11977	Sample	BE11977	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-066	COI	45	BE11977	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-066	CTGCTGCTATTGCTCATG	151.2599
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11979_Aamu_Bax2006_COI	GLTB-068	1	BE11979_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	GCACAACCGGGATCTTTACTTC	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11979_Pcan_Bot2011_IGS	GLTB-068	1	BE11979_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-068	IGS	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	CGTAATCCTCAGTACTGGCTA	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11979_Pper_Dia2013_COI	GLTB-068	1	BE11979_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	CTTAGTGGCATTAATTCGDAATCC	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11979_Sspa_Oph2007_COI	GLTB-068	1	BE11979_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	GAAGGCCTTCCGATGATC	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11979_Upin_Bot2011_COI	GLTB-068	1	BE11979_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	CTTTAATTACAGCGTTTTTATTGTTGT	151.25338
BE11979	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82838	WGS84	BE11979	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11979_Vgib_Wil2025_COI	GLTB-068	1	BE11979_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11979	Sample	BE11979	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-068	COI	45	BE11979	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-068	CCCCTCTTTACAAGTTCTTTAGGGT	151.25338
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11980_Cmae_Bot2011_COI	GLTB-069	1	BE11980_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	ATGAACAGTCTATCCTCCTTTAG	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11980_Hsan_Wil2021_COI	GLTB-069	1	BE11980_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	CCTGGGCCGGTATAGTAGGT	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11980_Mros_Gib2022_COI	GLTB-069	1	BE11980_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11980_Msal_Bot2012_COI	GLTB-069	1	BE11980_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	GYTAGTTCCRATGATGTTAGCTG	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11980_Mstr_Wil2021_COI	GLTB-069	1	BE11980_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	GTAGTGGTAACTACTCATGCTTTA	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11980_Pvir_Dia2013_COI	GLTB-069	1	BE11980_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	CTTAGTGGCATTAATTCGDAATCC	151.25566
BE11980	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.82818	WGS84	BE11980	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	High (Scale factor > 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11980_Rhar_Sim2018_COI	GLTB-069	1	BE11980_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11980	Sample	BE11980	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-069	COI	45	BE11980	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-069	CTGCTGCTATTGCTCATG	151.25566
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11982_Aamu_Bax2006_COI	GLTB-036	1	BE11982_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	GCACAACCGGGATCTTTACTTC	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11982_Pcan_Bot2011_IGS	GLTB-036	1	BE11982_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-036	IGS	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	CGTAATCCTCAGTACTGGCTA	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11982_Pper_Dia2013_COI	GLTB-036	1	BE11982_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	CTTAGTGGCATTAATTCGDAATCC	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11982_Sspa_Oph2007_COI	GLTB-036	1	BE11982_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	GAAGGCCTTCCGATGATC	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11982_Upin_Bot2011_COI	GLTB-036	1	BE11982_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	CTTTAATTACAGCGTTTTTATTGTTGT	151.27472
BE11982	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83858	WGS84	BE11982	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11982_Vgib_Wil2025_COI	GLTB-036	1	BE11982_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11982	Sample	BE11982	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-036	COI	45	BE11982	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-036	CCCCTCTTTACAAGTTCTTTAGGGT	151.27472
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11983_Asen_Wil2025_COI	GLTB-037	1	BE11983_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	GTTGTTGTTACAACACATGCTCTTTTG	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11983_Cjap_Sim2018_COI	GLTB-037	1	BE11983_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11983_Esin_And2018_Cyb	GLTB-037	1	BE11983_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-037	Cytb	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	ACCCCTCCTCATATCCAACCA	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11983_Mgig_Bot2012_COI	GLTB-037	1	BE11983_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	TCTTATTCGTTGGAGACTTTATAACCCT	151.27454
BE11983	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83762	WGS84	BE11983	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11983_Mjap_Gib2022_COI	GLTB-037	1	BE11983_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11983	Sample	BE11983	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-037	COI	45	BE11983	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-037	AATTTAATTGTGACTAGGCATGGTCTG	151.27454
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11984_Cmae_Bot2011_COI	GLTB-038	1	BE11984_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	ATGAACAGTCTATCCTCCTTTAG	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11984_Hsan_Wil2021_COI	GLTB-038	1	BE11984_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	CCTGGGCCGGTATAGTAGGT	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11984_Mros_Gib2022_COI	GLTB-038	1	BE11984_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11984_Msal_Bot2012_COI	GLTB-038	1	BE11984_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	GYTAGTTCCRATGATGTTAGCTG	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11984_Mstr_Wil2021_COI	GLTB-038	1	BE11984_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	GTAGTGGTAACTACTCATGCTTTA	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11984_Pvir_Dia2013_COI	GLTB-038	1	BE11984_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	CTTAGTGGCATTAATTCGDAATCC	151.27761
BE11984	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83676	WGS84	BE11984	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11984_Rhar_Sim2018_COI	GLTB-038	1	BE11984_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11984	Sample	BE11984	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-038	COI	45	BE11984	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-038	CTGCTGCTATTGCTCATG	151.27761
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11986_Aamu_Bax2006_COI	GLTB-040	1	BE11986_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	GCACAACCGGGATCTTTACTTC	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11986_Pcan_Bot2011_IGS	GLTB-040	1	BE11986_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-040	IGS	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	CGTAATCCTCAGTACTGGCTA	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11986_Pper_Dia2013_COI	GLTB-040	1	BE11986_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	CTTAGTGGCATTAATTCGDAATCC	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11986_Sspa_Oph2007_COI	GLTB-040	1	BE11986_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	GAAGGCCTTCCGATGATC	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11986_Upin_Bot2011_COI	GLTB-040	1	BE11986_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	CTTTAATTACAGCGTTTTTATTGTTGT	151.27501
BE11986	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16440	-23.83454	WGS84	BE11986	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT02_23-24_B	16/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11986_Vgib_Wil2025_COI	GLTB-040	1	BE11986_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11986	Sample	BE11986	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-040	COI	45	BE11986	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-040	CCCCTCTTTACAAGTTCTTTAGGGT	151.27501
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11991_Cmae_Bot2011_COI	GLTB-071	1	BE11991_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	ATGAACAGTCTATCCTCCTTTAG	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11991_Hsan_Wil2021_COI	GLTB-071	1	BE11991_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	CCTGGGCCGGTATAGTAGGT	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11991_Mros_Gib2022_COI	GLTB-071	1	BE11991_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11991_Msal_Bot2012_COI	GLTB-071	1	BE11991_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	GYTAGTTCCRATGATGTTAGCTG	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11991_Mstr_Wil2021_COI	GLTB-071	1	BE11991_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	GTAGTGGTAACTACTCATGCTTTA	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11991_Pvir_Dia2013_COI	GLTB-071	1	BE11991_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	CTTAGTGGCATTAATTCGDAATCC	151.18361
BE11991	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79578	WGS84	BE11991	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Moderate (Scale factor 5 - 10)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11991_Rhar_Sim2018_COI	GLTB-071	1	BE11991_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11991	Sample	BE11991	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-071	COI	45	BE11991	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-071	CTGCTGCTATTGCTCATG	151.18361
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11993_Aamu_Bax2006_COI	GLTB-073	1	BE11993_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	GCACAACCGGGATCTTTACTTC	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11993_Pcan_Bot2011_IGS	GLTB-073	1	BE11993_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-073	IGS	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	CGTAATCCTCAGTACTGGCTA	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11993_Pper_Dia2013_COI	GLTB-073	1	BE11993_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	CTTAGTGGCATTAATTCGDAATCC	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11993_Sspa_Oph2007_COI	GLTB-073	1	BE11993_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	GAAGGCCTTCCGATGATC	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11993_Upin_Bot2011_COI	GLTB-073	1	BE11993_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	CTTTAATTACAGCGTTTTTATTGTTGT	151.18557
BE11993	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79428	WGS84	BE11993	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11993_Vgib_Wil2025_COI	GLTB-073	1	BE11993_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11993	Sample	BE11993	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-073	COI	45	BE11993	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-073	CCCCTCTTTACAAGTTCTTTAGGGT	151.18557
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11994_Asen_Wil2025_COI	GLTB-074	1	BE11994_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	GTTGTTGTTACAACACATGCTCTTTTG	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11994_Cjap_Sim2018_COI	GLTB-074	1	BE11994_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11994_Esin_And2018_Cyb	GLTB-074	1	BE11994_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-074	Cytb	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	ACCCCTCCTCATATCCAACCA	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11994_Mgig_Bot2012_COI	GLTB-074	1	BE11994_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	TCTTATTCGTTGGAGACTTTATAACCCT	151.18773
BE11994	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79381	WGS84	BE11994	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11994_Mjap_Gib2022_COI	GLTB-074	1	BE11994_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11994	Sample	BE11994	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-074	COI	45	BE11994	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-074	AATTTAATTGTGACTAGGCATGGTCTG	151.18773
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11995_Cmae_Bot2011_COI	GLTB-075	1	BE11995_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	ATGAACAGTCTATCCTCCTTTAG	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11995_Hsan_Wil2021_COI	GLTB-075	1	BE11995_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	CCTGGGCCGGTATAGTAGGT	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11995_Mros_Gib2022_COI	GLTB-075	1	BE11995_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11995_Msal_Bot2012_COI	GLTB-075	1	BE11995_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	GYTAGTTCCRATGATGTTAGCTG	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11995_Mstr_Wil2021_COI	GLTB-075	1	BE11995_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	GTAGTGGTAACTACTCATGCTTTA	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11995_Pvir_Dia2013_COI	GLTB-075	1	BE11995_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	CTTAGTGGCATTAATTCGDAATCC	151.18169
BE11995	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79351	WGS84	BE11995	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11995_Rhar_Sim2018_COI	GLTB-075	1	BE11995_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11995	Sample	BE11995	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-075	COI	45	BE11995	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-075	CTGCTGCTATTGCTCATG	151.18169
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE11997_Aamu_Bax2006_COI	GLTB-077	1	BE11997_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	GCACAACCGGGATCTTTACTTC	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE11997_Pcan_Bot2011_IGS	GLTB-077	1	BE11997_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-077	IGS	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	CGTAATCCTCAGTACTGGCTA	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE11997_Pper_Dia2013_COI	GLTB-077	1	BE11997_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	CTTAGTGGCATTAATTCGDAATCC	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE11997_Sspa_Oph2007_COI	GLTB-077	1	BE11997_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	GAAGGCCTTCCGATGATC	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE11997_Upin_Bot2011_COI	GLTB-077	1	BE11997_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	CTTTAATTACAGCGTTTTTATTGTTGT	151.18751
BE11997	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79299	WGS84	BE11997	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE11997_Vgib_Wil2025_COI	GLTB-077	1	BE11997_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11997	Sample	BE11997	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-077	COI	45	BE11997	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-077	CCCCTCTTTACAAGTTCTTTAGGGT	151.18751
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE11998_Asen_Wil2025_COI	GLTB-078	1	BE11998_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	GTTGTTGTTACAACACATGCTCTTTTG	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE11998_Cjap_Sim2018_COI	GLTB-078	1	BE11998_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE11998_Esin_And2018_Cyb	GLTB-078	1	BE11998_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-078	Cytb	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	ACCCCTCCTCATATCCAACCA	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE11998_Mgig_Bot2012_COI	GLTB-078	1	BE11998_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	TCTTATTCGTTGGAGACTTTATAACCCT	151.18853
BE11998	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7922	WGS84	BE11998	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE11998_Mjap_Gib2022_COI	GLTB-078	1	BE11998_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11998	Sample	BE11998	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-078	COI	45	BE11998	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-078	AATTTAATTGTGACTAGGCATGGTCTG	151.18853
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE11999_Cmae_Bot2011_COI	GLTB-079	1	BE11999_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	ATGAACAGTCTATCCTCCTTTAG	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE11999_Hsan_Wil2021_COI	GLTB-079	1	BE11999_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	CCTGGGCCGGTATAGTAGGT	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE11999_Mros_Gib2022_COI	GLTB-079	1	BE11999_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE11999_Msal_Bot2012_COI	GLTB-079	1	BE11999_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	GYTAGTTCCRATGATGTTAGCTG	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE11999_Mstr_Wil2021_COI	GLTB-079	1	BE11999_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	GTAGTGGTAACTACTCATGCTTTA	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE11999_Pvir_Dia2013_COI	GLTB-079	1	BE11999_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	CTTAGTGGCATTAATTCGDAATCC	151.18068
BE11999	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79225	WGS84	BE11999	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE11999_Rhar_Sim2018_COI	GLTB-079	1	BE11999_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE11999	Sample	BE11999	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-079	COI	45	BE11999	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-079	CTGCTGCTATTGCTCATG	151.18068
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12000_Aamu_Bax2006_COI	GLTB-080	1	BE12000_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	GCACAACCGGGATCTTTACTTC	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12000_Pcan_Bot2011_IGS	GLTB-080	1	BE12000_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-080	IGS	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	CGTAATCCTCAGTACTGGCTA	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12000_Pper_Dia2013_COI	GLTB-080	1	BE12000_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	CTTAGTGGCATTAATTCGDAATCC	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12000_Sspa_Oph2007_COI	GLTB-080	1	BE12000_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	GAAGGCCTTCCGATGATC	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12000_Upin_Bot2011_COI	GLTB-080	1	BE12000_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	CTTTAATTACAGCGTTTTTATTGTTGT	151.18576
BE12000	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79178	WGS84	BE12000	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12000_Vgib_Wil2025_COI	GLTB-080	1	BE12000_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12000	Sample	BE12000	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-080	COI	45	BE12000	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-080	CCCCTCTTTACAAGTTCTTTAGGGT	151.18576
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12001_Asen_Wil2025_COI	GLTB-081	1	BE12001_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	GTTGTTGTTACAACACATGCTCTTTTG	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12001_Cjap_Sim2018_COI	GLTB-081	1	BE12001_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12001_Esin_And2018_Cyb	GLTB-081	1	BE12001_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-081	Cytb	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	ACCCCTCCTCATATCCAACCA	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12001_Mgig_Bot2012_COI	GLTB-081	1	BE12001_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	TCTTATTCGTTGGAGACTTTATAACCCT	151.1876
BE12001	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79102	WGS84	BE12001	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12001_Mjap_Gib2022_COI	GLTB-081	1	BE12001_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12001	Sample	BE12001	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-081	COI	45	BE12001	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-081	AATTTAATTGTGACTAGGCATGGTCTG	151.1876
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12002_Cmae_Bot2011_COI	GLTB-082	1	BE12002_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	ATGAACAGTCTATCCTCCTTTAG	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12002_Hsan_Wil2021_COI	GLTB-082	1	BE12002_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	CCTGGGCCGGTATAGTAGGT	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12002_Mros_Gib2022_COI	GLTB-082	1	BE12002_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12002_Msal_Bot2012_COI	GLTB-082	1	BE12002_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	GYTAGTTCCRATGATGTTAGCTG	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12002_Mstr_Wil2021_COI	GLTB-082	1	BE12002_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	GTAGTGGTAACTACTCATGCTTTA	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12002_Pvir_Dia2013_COI	GLTB-082	1	BE12002_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	CTTAGTGGCATTAATTCGDAATCC	151.18299
BE12002	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.79054	WGS84	BE12002	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12002_Rhar_Sim2018_COI	GLTB-082	1	BE12002_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12002	Sample	BE12002	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-082	COI	45	BE12002	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-082	CTGCTGCTATTGCTCATG	151.18299
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12004_Aamu_Bax2006_COI	GLTB-084	1	BE12004_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	GCACAACCGGGATCTTTACTTC	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12004_Pcan_Bot2011_IGS	GLTB-084	1	BE12004_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-084	IGS	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	CGTAATCCTCAGTACTGGCTA	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12004_Pper_Dia2013_COI	GLTB-084	1	BE12004_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	CTTAGTGGCATTAATTCGDAATCC	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12004_Sspa_Oph2007_COI	GLTB-084	1	BE12004_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	GAAGGCCTTCCGATGATC	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12004_Upin_Bot2011_COI	GLTB-084	1	BE12004_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	CTTTAATTACAGCGTTTTTATTGTTGT	151.18668
BE12004	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78973	WGS84	BE12004	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12004_Vgib_Wil2025_COI	GLTB-084	1	BE12004_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12004	Sample	BE12004	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-084	COI	45	BE12004	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-084	CCCCTCTTTACAAGTTCTTTAGGGT	151.18668
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12005_Asen_Wil2025_COI	GLTB-085	1	BE12005_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	GTTGTTGTTACAACACATGCTCTTTTG	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12005_Cjap_Sim2018_COI	GLTB-085	1	BE12005_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12005_Esin_And2018_Cyb	GLTB-085	1	BE12005_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-085	Cytb	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	ACCCCTCCTCATATCCAACCA	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12005_Mgig_Bot2012_COI	GLTB-085	1	BE12005_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	TCTTATTCGTTGGAGACTTTATAACCCT	151.18209
BE12005	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78938	WGS84	BE12005	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12005_Mjap_Gib2022_COI	GLTB-085	1	BE12005_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12005	Sample	BE12005	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-085	COI	45	BE12005	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-085	AATTTAATTGTGACTAGGCATGGTCTG	151.18209
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12006_Cmae_Bot2011_COI	GLTB-086	1	BE12006_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	ATGAACAGTCTATCCTCCTTTAG	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12006_Hsan_Wil2021_COI	GLTB-086	1	BE12006_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	CCTGGGCCGGTATAGTAGGT	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12006_Mros_Gib2022_COI	GLTB-086	1	BE12006_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12006_Msal_Bot2012_COI	GLTB-086	1	BE12006_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	GYTAGTTCCRATGATGTTAGCTG	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12006_Mstr_Wil2021_COI	GLTB-086	1	BE12006_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	GTAGTGGTAACTACTCATGCTTTA	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12006_Pvir_Dia2013_COI	GLTB-086	1	BE12006_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	CTTAGTGGCATTAATTCGDAATCC	151.18354
BE12006	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78912	WGS84	BE12006	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12006_Rhar_Sim2018_COI	GLTB-086	1	BE12006_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12006	Sample	BE12006	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-086	COI	45	BE12006	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-086	CTGCTGCTATTGCTCATG	151.18354
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12008_Aamu_Bax2006_COI	GLTB-088	1	BE12008_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	GCACAACCGGGATCTTTACTTC	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12008_Pcan_Bot2011_IGS	GLTB-088	1	BE12008_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-088	IGS	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	CGTAATCCTCAGTACTGGCTA	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12008_Pper_Dia2013_COI	GLTB-088	1	BE12008_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	CTTAGTGGCATTAATTCGDAATCC	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12008_Sspa_Oph2007_COI	GLTB-088	1	BE12008_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	GAAGGCCTTCCGATGATC	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12008_Upin_Bot2011_COI	GLTB-088	1	BE12008_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	CTTTAATTACAGCGTTTTTATTGTTGT	151.18112
BE12008	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78842	WGS84	BE12008	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12008_Vgib_Wil2025_COI	GLTB-088	1	BE12008_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12008	Sample	BE12008	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-088	COI	45	BE12008	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-088	CCCCTCTTTACAAGTTCTTTAGGGT	151.18112
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12009_Asen_Wil2025_COI	GLTB-089	1	BE12009_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	GTTGTTGTTACAACACATGCTCTTTTG	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12009_Cjap_Sim2018_COI	GLTB-089	1	BE12009_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12009_Esin_And2018_Cyb	GLTB-089	1	BE12009_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-089	Cytb	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	ACCCCTCCTCATATCCAACCA	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12009_Mgig_Bot2012_COI	GLTB-089	1	BE12009_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	TCTTATTCGTTGGAGACTTTATAACCCT	151.18263
BE12009	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78775	WGS84	BE12009	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12009_Mjap_Gib2022_COI	GLTB-089	1	BE12009_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12009	Sample	BE12009	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-089	COI	45	BE12009	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-089	AATTTAATTGTGACTAGGCATGGTCTG	151.18263
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12011_Aamu_Bax2006_COI	GLTB-091	1	BE12011_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	GCACAACCGGGATCTTTACTTC	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12011_Pcan_Bot2011_IGS	GLTB-091	1	BE12011_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-091	IGS	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	CGTAATCCTCAGTACTGGCTA	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12011_Pper_Dia2013_COI	GLTB-091	1	BE12011_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	CTTAGTGGCATTAATTCGDAATCC	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12011_Sspa_Oph2007_COI	GLTB-091	1	BE12011_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	GAAGGCCTTCCGATGATC	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12011_Upin_Bot2011_COI	GLTB-091	1	BE12011_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	CTTTAATTACAGCGTTTTTATTGTTGT	151.17847
BE12011	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.7874	WGS84	BE12011	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12011_Vgib_Wil2025_COI	GLTB-091	1	BE12011_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12011	Sample	BE12011	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-091	COI	45	BE12011	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-091	CCCCTCTTTACAAGTTCTTTAGGGT	151.17847
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12012_Asen_Wil2025_COI	GLTB-092	1	BE12012_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	GTTGTTGTTACAACACATGCTCTTTTG	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12012_Cjap_Sim2018_COI	GLTB-092	1	BE12012_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12012_Esin_And2018_Cyb	GLTB-092	1	BE12012_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-092	Cytb	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	ACCCCTCCTCATATCCAACCA	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12012_Mgig_Bot2012_COI	GLTB-092	1	BE12012_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	TCTTATTCGTTGGAGACTTTATAACCCT	151.17995
BE12012	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78684	WGS84	BE12012	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12012_Mjap_Gib2022_COI	GLTB-092	1	BE12012_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12012	Sample	BE12012	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-092	COI	45	BE12012	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-092	AATTTAATTGTGACTAGGCATGGTCTG	151.17995
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12013_Cmae_Bot2011_COI	GLTB-093	1	BE12013_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	ATGAACAGTCTATCCTCCTTTAG	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12013_Hsan_Wil2021_COI	GLTB-093	1	BE12013_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	CCTGGGCCGGTATAGTAGGT	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12013_Mros_Gib2022_COI	GLTB-093	1	BE12013_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12013_Msal_Bot2012_COI	GLTB-093	1	BE12013_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	GYTAGTTCCRATGATGTTAGCTG	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12013_Mstr_Wil2021_COI	GLTB-093	1	BE12013_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	GTAGTGGTAACTACTCATGCTTTA	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12013_Pvir_Dia2013_COI	GLTB-093	1	BE12013_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	CTTAGTGGCATTAATTCGDAATCC	151.18144
BE12013	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78672	WGS84	BE12013	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12013_Rhar_Sim2018_COI	GLTB-093	1	BE12013_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12013	Sample	BE12013	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-093	COI	45	BE12013	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-093	CTGCTGCTATTGCTCATG	151.18144
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12015_Aamu_Bax2006_COI	GLTB-095	1	BE12015_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	GCACAACCGGGATCTTTACTTC	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12015_Pcan_Bot2011_IGS	GLTB-095	1	BE12015_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-095	IGS	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	CGTAATCCTCAGTACTGGCTA	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12015_Pper_Dia2013_COI	GLTB-095	1	BE12015_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	CTTAGTGGCATTAATTCGDAATCC	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12015_Sspa_Oph2007_COI	GLTB-095	1	BE12015_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	GAAGGCCTTCCGATGATC	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12015_Upin_Bot2011_COI	GLTB-095	1	BE12015_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	CTTTAATTACAGCGTTTTTATTGTTGT	151.17895
BE12015	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78567	WGS84	BE12015	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12015_Vgib_Wil2025_COI	GLTB-095	1	BE12015_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12015	Sample	BE12015	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-095	COI	45	BE12015	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-095	CCCCTCTTTACAAGTTCTTTAGGGT	151.17895
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12016_Asen_Wil2025_COI	GLTB-096	1	BE12016_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	GTTGTTGTTACAACACATGCTCTTTTG	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12016_Cjap_Sim2018_COI	GLTB-096	1	BE12016_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12016_Esin_And2018_Cyb	GLTB-096	1	BE12016_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-096	Cytb	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	ACCCCTCCTCATATCCAACCA	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12016_Mgig_Bot2012_COI	GLTB-096	1	BE12016_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	TCTTATTCGTTGGAGACTTTATAACCCT	151.18077
BE12016	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78549	WGS84	BE12016	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12016_Mjap_Gib2022_COI	GLTB-096	1	BE12016_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12016	Sample	BE12016	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-096	COI	45	BE12016	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-096	AATTTAATTGTGACTAGGCATGGTCTG	151.18077
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12017_Cmae_Bot2011_COI	GLTB-097	1	BE12017_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	ATGAACAGTCTATCCTCCTTTAG	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12017_Hsan_Wil2021_COI	GLTB-097	1	BE12017_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	CCTGGGCCGGTATAGTAGGT	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12017_Mros_Gib2022_COI	GLTB-097	1	BE12017_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12017_Msal_Bot2012_COI	GLTB-097	1	BE12017_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	GYTAGTTCCRATGATGTTAGCTG	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12017_Mstr_Wil2021_COI	GLTB-097	1	BE12017_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	GTAGTGGTAACTACTCATGCTTTA	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12017_Pvir_Dia2013_COI	GLTB-097	1	BE12017_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	CTTAGTGGCATTAATTCGDAATCC	151.17635
BE12017	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78474	WGS84	BE12017	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12017_Rhar_Sim2018_COI	GLTB-097	1	BE12017_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12017	Sample	BE12017	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-097	COI	45	BE12017	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-097	CTGCTGCTATTGCTCATG	151.17635
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12019_Aamu_Bax2006_COI	GLTB-099	1	BE12019_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	GCACAACCGGGATCTTTACTTC	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12019_Pcan_Bot2011_IGS	GLTB-099	1	BE12019_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-099	IGS	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	CGTAATCCTCAGTACTGGCTA	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12019_Pper_Dia2013_COI	GLTB-099	1	BE12019_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	CTTAGTGGCATTAATTCGDAATCC	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12019_Sspa_Oph2007_COI	GLTB-099	1	BE12019_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	GAAGGCCTTCCGATGATC	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12019_Upin_Bot2011_COI	GLTB-099	1	BE12019_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	CTTTAATTACAGCGTTTTTATTGTTGT	151.17342
BE12019	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78364	WGS84	BE12019	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12019_Vgib_Wil2025_COI	GLTB-099	1	BE12019_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12019	Sample	BE12019	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-099	COI	45	BE12019	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-099	CCCCTCTTTACAAGTTCTTTAGGGT	151.17342
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12020_Cmae_Bot2011_COI	GLTB-100	1	BE12020_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	ATGAACAGTCTATCCTCCTTTAG	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12020_Hsan_Wil2021_COI	GLTB-100	1	BE12020_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	CCTGGGCCGGTATAGTAGGT	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12020_Mros_Gib2022_COI	GLTB-100	1	BE12020_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12020_Msal_Bot2012_COI	GLTB-100	1	BE12020_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	GYTAGTTCCRATGATGTTAGCTG	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12020_Mstr_Wil2021_COI	GLTB-100	1	BE12020_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	GTAGTGGTAACTACTCATGCTTTA	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12020_Pvir_Dia2013_COI	GLTB-100	1	BE12020_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	CTTAGTGGCATTAATTCGDAATCC	151.17533
BE12020	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78361	WGS84	BE12020	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12020_Rhar_Sim2018_COI	GLTB-100	1	BE12020_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12020	Sample	BE12020	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-100	COI	45	BE12020	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-100	CTGCTGCTATTGCTCATG	151.17533
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE12022_Aamu_Bax2006_COI	GLTB-102	1	BE12022_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Aamu_Bax2006_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	GCACAACCGGGATCTTTACTTC	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE12022_Pcan_Bot2011_IGS	GLTB-102	1	BE12022_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Pcan_Bot2011_IGS	GLTB-102	IGS	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	CGTAATCCTCAGTACTGGCTA	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE12022_Pper_Dia2013_COI	GLTB-102	1	BE12022_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Pper_Dia2013_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	CTTAGTGGCATTAATTCGDAATCC	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE12022_Sspa_Oph2007_COI	GLTB-102	1	BE12022_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Sspa_Oph2007_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	GAAGGCCTTCCGATGATC	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE12022_Upin_Bot2011_COI	GLTB-102	1	BE12022_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Upin_Bot2011_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	CTTTAATTACAGCGTTTTTATTGTTGT	151.17257
BE12022	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78234	WGS84	BE12022	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE12022_Vgib_Wil2025_COI	GLTB-102	1	BE12022_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12022	Sample	BE12022	targeted	Absent	Sample	Vgib_Wil2025_COI	GLTB-102	COI	45	BE12022	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-102	CCCCTCTTTACAAGTTCTTTAGGGT	151.17257
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE12023_Asen_Wil2025_COI	GLTB-103	1	BE12023_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Asen_Wil2025_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	GTTGTTGTTACAACACATGCTCTTTTG	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE12023_Cjap_Sim2018_COI	GLTB-103	1	BE12023_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Cjap_Sim2018_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	TTAATATACGGTCATTTGGTATGAGTATAGATC	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE12023_Esin_And2018_Cyb	GLTB-103	1	BE12023_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Esin_And2018_Cyb	GLTB-103	Cytb	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	ACCCCTCCTCATATCCAACCA	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE12023_Mgig_Bot2012_COI	GLTB-103	1	BE12023_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Mgig_Bot2012_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	TCTTATTCGTTGGAGACTTTATAACCCT	151.17423
BE12023	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78245	WGS84	BE12023	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE12023_Mjap_Gib2022_COI	GLTB-103	1	BE12023_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12023	Sample	BE12023	targeted	Absent	Sample	Mjap_Gib2022_COI	GLTB-103	COI	45	BE12023	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-103	AATTTAATTGTGACTAGGCATGGTCTG	151.17423
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE12024_Cmae_Bot2011_COI	GLTB-104	1	BE12024_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Cmae_Bot2011_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	ATGAACAGTCTATCCTCCTTTAG	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE12024_Hsan_Wil2021_COI	GLTB-104	1	BE12024_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Hsan_Wil2021_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	CCTGGGCCGGTATAGTAGGT	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE12024_Mros_Gib2022_COI	GLTB-104	1	BE12024_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Mros_Gib2022_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	ACTTCTACCTCCTGCTCTTCTACTTCTC	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE12024_Msal_Bot2012_COI	GLTB-104	1	BE12024_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Msal_Bot2012_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	GYTAGTTCCRATGATGTTAGCTG	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE12024_Mstr_Wil2021_COI	GLTB-104	1	BE12024_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Mstr_Wil2021_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	GTAGTGGTAACTACTCATGCTTTA	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE12024_Pvir_Dia2013_COI	GLTB-104	1	BE12024_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Pvir_Dia2013_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	CTTAGTGGCATTAATTCGDAATCC	151.17575
BE12024	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16445	-23.78198	WGS84	BE12024	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Minor (Scale factor 2 - 5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-GLT03_23-24_B	17/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE12024_Rhar_Sim2018_COI	GLTB-104	1	BE12024_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE12024	Sample	BE12024	targeted	Absent	Sample	Rhar_Sim2018_COI	GLTB-104	COI	45	BE12024	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	GLTB-104	CTGCTGCTATTGCTCATG	151.17575
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32692_Asen_Wil2025_COI	CNSB-001	1	BE32692_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	GTTGTTGTTACAACACATGCTCTTTTG	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32692_Cjap_Sim2018_COI	CNSB-001	1	BE32692_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32692_Esin_And2018_Cyb	CNSB-001	1	BE32692_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-001	Cytb	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	ACCCCTCCTCATATCCAACCA	145.77391
BE32692	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.95044	WGS84	BE32692	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32692_Mjap_Gib2022_COI	CNSB-001	1	BE32692_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32692	Sample	BE32692	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-001	COI	45	BE32692	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-001	AATTTAATTGTGACTAGGCATGGTCTG	145.77391
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32693_Cmae_Bot2011_COI	CNSB-002	1	BE32693_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	ATGAACAGTCTATCCTCCTTTAG	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32693_Hsan_Wil2021_COI	CNSB-002	1	BE32693_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	CCTGGGCCGGTATAGTAGGT	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32693_Mros_Gib2022_COI	CNSB-002	1	BE32693_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32693_Msal_Bot2012_COI	CNSB-002	1	BE32693_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	GYTAGTTCCRATGATGTTAGCTG	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32693_Mstr_Wil2021_COI	CNSB-002	1	BE32693_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	GTAGTGGTAACTACTCATGCTTTA	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32693_Pvir_Dia2013_COI	CNSB-002	1	BE32693_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	CTTAGTGGCATTAATTCGDAATCC	145.77343
BE32693	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94743	WGS84	BE32693	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32693_Rhar_Sim2018_COI	CNSB-002	1	BE32693_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32693	Sample	BE32693	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-002	COI	45	BE32693	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-002	CTGCTGCTATTGCTCATG	145.77343
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32695_Aamu_Bax2006_COI	CNSB-004	1	BE32695_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	GCACAACCGGGATCTTTACTTC	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32695_Pcan_Bot2011_IGS	CNSB-004	1	BE32695_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-004	IGS	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	CGTAATCCTCAGTACTGGCTA	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32695_Pper_Dia2013_COI	CNSB-004	1	BE32695_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	CTTAGTGGCATTAATTCGDAATCC	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32695_Sspa_Oph2007_COI	CNSB-004	1	BE32695_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	GAAGGCCTTCCGATGATC	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32695_Upin_Bot2011_COI	CNSB-004	1	BE32695_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	CTTTAATTACAGCGTTTTTATTGTTGT	145.7763
BE32695	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94265	WGS84	BE32695	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32695_Vgib_Wil2025_COI	CNSB-004	1	BE32695_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32695	Sample	BE32695	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-004	COI	45	BE32695	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-004	CCCCTCTTTACAAGTTCTTTAGGGT	145.7763
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32696_Asen_Wil2025_COI	CNSB-005	1	BE32696_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	GTTGTTGTTACAACACATGCTCTTTTG	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32696_Cjap_Sim2018_COI	CNSB-005	1	BE32696_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32696_Esin_And2018_Cyb	CNSB-005	1	BE32696_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-005	Cytb	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	ACCCCTCCTCATATCCAACCA	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32696_Mgig_Bot2012_COI	CNSB-005	1	BE32696_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	TCTTATTCGTTGGAGACTTTATAACCCT	145.77936
BE32696	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94192	WGS84	BE32696	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32696_Mjap_Gib2022_COI	CNSB-005	1	BE32696_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32696	Sample	BE32696	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-005	COI	45	BE32696	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-005	AATTTAATTGTGACTAGGCATGGTCTG	145.77936
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32697_Cmae_Bot2011_COI	CNSB-006	1	BE32697_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	ATGAACAGTCTATCCTCCTTTAG	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32697_Hsan_Wil2021_COI	CNSB-006	1	BE32697_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	CCTGGGCCGGTATAGTAGGT	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32697_Mros_Gib2022_COI	CNSB-006	1	BE32697_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32697_Msal_Bot2012_COI	CNSB-006	1	BE32697_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	GYTAGTTCCRATGATGTTAGCTG	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32697_Mstr_Wil2021_COI	CNSB-006	1	BE32697_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	GTAGTGGTAACTACTCATGCTTTA	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32697_Pvir_Dia2013_COI	CNSB-006	1	BE32697_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	CTTAGTGGCATTAATTCGDAATCC	145.7822
BE32697	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94177	WGS84	BE32697	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32697_Rhar_Sim2018_COI	CNSB-006	1	BE32697_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32697	Sample	BE32697	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-006	COI	45	BE32697	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-006	CTGCTGCTATTGCTCATG	145.7822
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32699_Aamu_Bax2006_COI	CNSB-008	1	BE32699_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	GCACAACCGGGATCTTTACTTC	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32699_Pcan_Bot2011_IGS	CNSB-008	1	BE32699_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-008	IGS	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	CGTAATCCTCAGTACTGGCTA	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32699_Pper_Dia2013_COI	CNSB-008	1	BE32699_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	CTTAGTGGCATTAATTCGDAATCC	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32699_Sspa_Oph2007_COI	CNSB-008	1	BE32699_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	GAAGGCCTTCCGATGATC	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32699_Upin_Bot2011_COI	CNSB-008	1	BE32699_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	CTTTAATTACAGCGTTTTTATTGTTGT	145.78279
BE32699	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.94032	WGS84	BE32699	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32699_Vgib_Wil2025_COI	CNSB-008	1	BE32699_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32699	Sample	BE32699	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-008	COI	45	BE32699	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-008	CCCCTCTTTACAAGTTCTTTAGGGT	145.78279
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32700_Asen_Wil2025_COI	CNSB-009	1	BE32700_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	GTTGTTGTTACAACACATGCTCTTTTG	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32700_Cjap_Sim2018_COI	CNSB-009	1	BE32700_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32700_Esin_And2018_Cyb	CNSB-009	1	BE32700_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-009	Cytb	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	ACCCCTCCTCATATCCAACCA	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32700_Mgig_Bot2012_COI	CNSB-009	1	BE32700_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	TCTTATTCGTTGGAGACTTTATAACCCT	145.78192
BE32700	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93896	WGS84	BE32700	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32700_Mjap_Gib2022_COI	CNSB-009	1	BE32700_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32700	Sample	BE32700	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-009	COI	45	BE32700	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-009	AATTTAATTGTGACTAGGCATGGTCTG	145.78192
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32701_Cmae_Bot2011_COI	CNSB-010	1	BE32701_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	ATGAACAGTCTATCCTCCTTTAG	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32701_Hsan_Wil2021_COI	CNSB-010	1	BE32701_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	CCTGGGCCGGTATAGTAGGT	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32701_Mros_Gib2022_COI	CNSB-010	1	BE32701_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32701_Msal_Bot2012_COI	CNSB-010	1	BE32701_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	GYTAGTTCCRATGATGTTAGCTG	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32701_Mstr_Wil2021_COI	CNSB-010	1	BE32701_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	GTAGTGGTAACTACTCATGCTTTA	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32701_Pvir_Dia2013_COI	CNSB-010	1	BE32701_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	CTTAGTGGCATTAATTCGDAATCC	145.78101
BE32701	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9376	WGS84	BE32701	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32701_Rhar_Sim2018_COI	CNSB-010	1	BE32701_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32701	Sample	BE32701	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-010	COI	45	BE32701	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-010	CTGCTGCTATTGCTCATG	145.78101
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32703_Aamu_Bax2006_COI	CNSB-012	1	BE32703_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	GCACAACCGGGATCTTTACTTC	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32703_Pcan_Bot2011_IGS	CNSB-012	1	BE32703_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-012	IGS	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	CGTAATCCTCAGTACTGGCTA	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32703_Pper_Dia2013_COI	CNSB-012	1	BE32703_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	CTTAGTGGCATTAATTCGDAATCC	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32703_Sspa_Oph2007_COI	CNSB-012	1	BE32703_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	GAAGGCCTTCCGATGATC	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32703_Upin_Bot2011_COI	CNSB-012	1	BE32703_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	CTTTAATTACAGCGTTTTTATTGTTGT	145.78168
BE32703	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93615	WGS84	BE32703	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32703_Vgib_Wil2025_COI	CNSB-012	1	BE32703_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32703	Sample	BE32703	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-012	COI	45	BE32703	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-012	CCCCTCTTTACAAGTTCTTTAGGGT	145.78168
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32704_Asen_Wil2025_COI	CNSB-013	1	BE32704_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	GTTGTTGTTACAACACATGCTCTTTTG	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32704_Cjap_Sim2018_COI	CNSB-013	1	BE32704_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32704_Esin_And2018_Cyb	CNSB-013	1	BE32704_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-013	Cytb	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	ACCCCTCCTCATATCCAACCA	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32704_Mgig_Bot2012_COI	CNSB-013	1	BE32704_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	TCTTATTCGTTGGAGACTTTATAACCCT	145.78072
BE32704	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93474	WGS84	BE32704	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32704_Mjap_Gib2022_COI	CNSB-013	1	BE32704_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32704	Sample	BE32704	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-013	COI	45	BE32704	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-013	AATTTAATTGTGACTAGGCATGGTCTG	145.78072
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32705_Cmae_Bot2011_COI	CNSB-014	1	BE32705_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	ATGAACAGTCTATCCTCCTTTAG	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32705_Hsan_Wil2021_COI	CNSB-014	1	BE32705_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	CCTGGGCCGGTATAGTAGGT	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32705_Mros_Gib2022_COI	CNSB-014	1	BE32705_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32705_Msal_Bot2012_COI	CNSB-014	1	BE32705_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	GYTAGTTCCRATGATGTTAGCTG	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32705_Mstr_Wil2021_COI	CNSB-014	1	BE32705_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	GTAGTGGTAACTACTCATGCTTTA	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32705_Pvir_Dia2013_COI	CNSB-014	1	BE32705_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	CTTAGTGGCATTAATTCGDAATCC	145.78204
BE32705	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93473	WGS84	BE32705	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32705_Rhar_Sim2018_COI	CNSB-014	1	BE32705_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32705	Sample	BE32705	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-014	COI	45	BE32705	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-014	CTGCTGCTATTGCTCATG	145.78204
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32707_Aamu_Bax2006_COI	CNSB-016	1	BE32707_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	GCACAACCGGGATCTTTACTTC	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32707_Pcan_Bot2011_IGS	CNSB-016	1	BE32707_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-016	IGS	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	CGTAATCCTCAGTACTGGCTA	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32707_Pper_Dia2013_COI	CNSB-016	1	BE32707_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	CTTAGTGGCATTAATTCGDAATCC	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32707_Sspa_Oph2007_COI	CNSB-016	1	BE32707_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	GAAGGCCTTCCGATGATC	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32707_Upin_Bot2011_COI	CNSB-016	1	BE32707_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	CTTTAATTACAGCGTTTTTATTGTTGT	145.78074
BE32707	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93209	WGS84	BE32707	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32707_Vgib_Wil2025_COI	CNSB-016	1	BE32707_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32707	Sample	BE32707	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-016	COI	45	BE32707	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-016	CCCCTCTTTACAAGTTCTTTAGGGT	145.78074
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32708_Asen_Wil2025_COI	CNSB-017	1	BE32708_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	GTTGTTGTTACAACACATGCTCTTTTG	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32708_Cjap_Sim2018_COI	CNSB-017	1	BE32708_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32708_Esin_And2018_Cyb	CNSB-017	1	BE32708_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-017	Cytb	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	ACCCCTCCTCATATCCAACCA	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32708_Mgig_Bot2012_COI	CNSB-017	1	BE32708_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	TCTTATTCGTTGGAGACTTTATAACCCT	145.7822
BE32708	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93179	WGS84	BE32708	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32708_Mjap_Gib2022_COI	CNSB-017	1	BE32708_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32708	Sample	BE32708	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-017	COI	45	BE32708	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-017	AATTTAATTGTGACTAGGCATGGTCTG	145.7822
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32709_Cmae_Bot2011_COI	CNSB-018	1	BE32709_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	ATGAACAGTCTATCCTCCTTTAG	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32709_Hsan_Wil2021_COI	CNSB-018	1	BE32709_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	CCTGGGCCGGTATAGTAGGT	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32709_Mros_Gib2022_COI	CNSB-018	1	BE32709_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32709_Msal_Bot2012_COI	CNSB-018	1	BE32709_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	GYTAGTTCCRATGATGTTAGCTG	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32709_Mstr_Wil2021_COI	CNSB-018	1	BE32709_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	GTAGTGGTAACTACTCATGCTTTA	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32709_Pvir_Dia2013_COI	CNSB-018	1	BE32709_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	CTTAGTGGCATTAATTCGDAATCC	145.78124
BE32709	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93046	WGS84	BE32709	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32709_Rhar_Sim2018_COI	CNSB-018	1	BE32709_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32709	Sample	BE32709	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-018	COI	45	BE32709	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-018	CTGCTGCTATTGCTCATG	145.78124
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32710_Aamu_Bax2006_COI	CNSB-019	1	BE32710_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	GCACAACCGGGATCTTTACTTC	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32710_Pcan_Bot2011_IGS	CNSB-019	1	BE32710_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-019	IGS	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	CGTAATCCTCAGTACTGGCTA	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32710_Pper_Dia2013_COI	CNSB-019	1	BE32710_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	CTTAGTGGCATTAATTCGDAATCC	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32710_Sspa_Oph2007_COI	CNSB-019	1	BE32710_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	GAAGGCCTTCCGATGATC	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32710_Upin_Bot2011_COI	CNSB-019	1	BE32710_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	CTTTAATTACAGCGTTTTTATTGTTGT	145.78283
BE32710	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.93022	WGS84	BE32710	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32710_Vgib_Wil2025_COI	CNSB-019	1	BE32710_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32710	Sample	BE32710	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-019	COI	45	BE32710	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-019	CCCCTCTTTACAAGTTCTTTAGGGT	145.78283
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32711_Asen_Wil2025_COI	CNSB-020	1	BE32711_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	GTTGTTGTTACAACACATGCTCTTTTG	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32711_Cjap_Sim2018_COI	CNSB-020	1	BE32711_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32711_Esin_And2018_Cyb	CNSB-020	1	BE32711_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-020	Cytb	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	ACCCCTCCTCATATCCAACCA	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32711_Mgig_Bot2012_COI	CNSB-020	1	BE32711_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	TCTTATTCGTTGGAGACTTTATAACCCT	145.78186
BE32711	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92896	WGS84	BE32711	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32711_Mjap_Gib2022_COI	CNSB-020	1	BE32711_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32711	Sample	BE32711	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-020	COI	45	BE32711	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-020	AATTTAATTGTGACTAGGCATGGTCTG	145.78186
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32712_Cmae_Bot2011_COI	CNSB-021	1	BE32712_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	ATGAACAGTCTATCCTCCTTTAG	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32712_Hsan_Wil2021_COI	CNSB-021	1	BE32712_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	CCTGGGCCGGTATAGTAGGT	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32712_Mros_Gib2022_COI	CNSB-021	1	BE32712_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32712_Msal_Bot2012_COI	CNSB-021	1	BE32712_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	GYTAGTTCCRATGATGTTAGCTG	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32712_Mstr_Wil2021_COI	CNSB-021	1	BE32712_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	GTAGTGGTAACTACTCATGCTTTA	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32712_Pvir_Dia2013_COI	CNSB-021	1	BE32712_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	CTTAGTGGCATTAATTCGDAATCC	145.78355
BE32712	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92878	WGS84	BE32712	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32712_Rhar_Sim2018_COI	CNSB-021	1	BE32712_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32712	Sample	BE32712	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-021	COI	45	BE32712	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-021	CTGCTGCTATTGCTCATG	145.78355
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32714_Aamu_Bax2006_COI	CNSB-023	1	BE32714_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	GCACAACCGGGATCTTTACTTC	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32714_Pcan_Bot2011_IGS	CNSB-023	1	BE32714_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-023	IGS	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	CGTAATCCTCAGTACTGGCTA	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32714_Pper_Dia2013_COI	CNSB-023	1	BE32714_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	CTTAGTGGCATTAATTCGDAATCC	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32714_Sspa_Oph2007_COI	CNSB-023	1	BE32714_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	GAAGGCCTTCCGATGATC	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32714_Upin_Bot2011_COI	CNSB-023	1	BE32714_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	CTTTAATTACAGCGTTTTTATTGTTGT	145.78405
BE32714	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92783	WGS84	BE32714	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32714_Vgib_Wil2025_COI	CNSB-023	1	BE32714_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32714	Sample	BE32714	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-023	COI	45	BE32714	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-023	CCCCTCTTTACAAGTTCTTTAGGGT	145.78405
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32715_Asen_Wil2025_COI	CNSB-024	1	BE32715_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	GTTGTTGTTACAACACATGCTCTTTTG	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32715_Cjap_Sim2018_COI	CNSB-024	1	BE32715_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32715_Esin_And2018_Cyb	CNSB-024	1	BE32715_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-024	Cytb	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	ACCCCTCCTCATATCCAACCA	145.78184
BE32715	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92619	WGS84	BE32715	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32715_Mjap_Gib2022_COI	CNSB-024	1	BE32715_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32715	Sample	BE32715	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-024	COI	45	BE32715	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-024	AATTTAATTGTGACTAGGCATGGTCTG	145.78184
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32716_Cmae_Bot2011_COI	CNSB-025	1	BE32716_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	ATGAACAGTCTATCCTCCTTTAG	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32716_Hsan_Wil2021_COI	CNSB-025	1	BE32716_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	CCTGGGCCGGTATAGTAGGT	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32716_Mros_Gib2022_COI	CNSB-025	1	BE32716_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32716_Msal_Bot2012_COI	CNSB-025	1	BE32716_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	GYTAGTTCCRATGATGTTAGCTG	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32716_Mstr_Wil2021_COI	CNSB-025	1	BE32716_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	GTAGTGGTAACTACTCATGCTTTA	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32716_Pvir_Dia2013_COI	CNSB-025	1	BE32716_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	CTTAGTGGCATTAATTCGDAATCC	145.78279
BE32716	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92638	WGS84	BE32716	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32716_Rhar_Sim2018_COI	CNSB-025	1	BE32716_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32716	Sample	BE32716	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-025	COI	45	BE32716	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-025	CTGCTGCTATTGCTCATG	145.78279
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32718_Aamu_Bax2006_COI	CNSB-027	1	BE32718_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	GCACAACCGGGATCTTTACTTC	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32718_Pcan_Bot2011_IGS	CNSB-027	1	BE32718_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-027	IGS	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	CGTAATCCTCAGTACTGGCTA	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32718_Pper_Dia2013_COI	CNSB-027	1	BE32718_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	CTTAGTGGCATTAATTCGDAATCC	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32718_Sspa_Oph2007_COI	CNSB-027	1	BE32718_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	GAAGGCCTTCCGATGATC	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32718_Upin_Bot2011_COI	CNSB-027	1	BE32718_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	CTTTAATTACAGCGTTTTTATTGTTGT	145.78385
BE32718	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92448	WGS84	BE32718	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32718_Vgib_Wil2025_COI	CNSB-027	1	BE32718_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32718	Sample	BE32718	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-027	COI	45	BE32718	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-027	CCCCTCTTTACAAGTTCTTTAGGGT	145.78385
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32719_Asen_Wil2025_COI	CNSB-028	1	BE32719_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	GTTGTTGTTACAACACATGCTCTTTTG	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32719_Cjap_Sim2018_COI	CNSB-028	1	BE32719_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32719_Esin_And2018_Cyb	CNSB-028	1	BE32719_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-028	Cytb	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	ACCCCTCCTCATATCCAACCA	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32719_Mgig_Bot2012_COI	CNSB-028	1	BE32719_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	TCTTATTCGTTGGAGACTTTATAACCCT	145.78334
BE32719	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92322	WGS84	BE32719	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32719_Mjap_Gib2022_COI	CNSB-028	1	BE32719_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32719	Sample	BE32719	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-028	COI	45	BE32719	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-028	AATTTAATTGTGACTAGGCATGGTCTG	145.78334
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32721_Aamu_Bax2006_COI	CNSB-030	1	BE32721_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	GCACAACCGGGATCTTTACTTC	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32721_Pcan_Bot2011_IGS	CNSB-030	1	BE32721_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-030	IGS	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	CGTAATCCTCAGTACTGGCTA	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32721_Pper_Dia2013_COI	CNSB-030	1	BE32721_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	CTTAGTGGCATTAATTCGDAATCC	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32721_Sspa_Oph2007_COI	CNSB-030	1	BE32721_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	GAAGGCCTTCCGATGATC	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32721_Upin_Bot2011_COI	CNSB-030	1	BE32721_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	CTTTAATTACAGCGTTTTTATTGTTGT	145.78369
BE32721	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32721	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32721_Vgib_Wil2025_COI	CNSB-030	1	BE32721_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32721	Sample	BE32721	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-030	COI	45	BE32721	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-030	CCCCTCTTTACAAGTTCTTTAGGGT	145.78369
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32722_Asen_Wil2025_COI	CNSB-031	1	BE32722_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	GTTGTTGTTACAACACATGCTCTTTTG	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32722_Cjap_Sim2018_COI	CNSB-031	1	BE32722_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32722_Esin_And2018_Cyb	CNSB-031	1	BE32722_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-031	Cytb	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	ACCCCTCCTCATATCCAACCA	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32722_Mgig_Bot2012_COI	CNSB-031	1	BE32722_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	TCTTATTCGTTGGAGACTTTATAACCCT	145.78513
BE32722	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92154	WGS84	BE32722	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32722_Mjap_Gib2022_COI	CNSB-031	1	BE32722_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32722	Sample	BE32722	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-031	COI	45	BE32722	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-031	AATTTAATTGTGACTAGGCATGGTCTG	145.78513
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32723_Cmae_Bot2011_COI	CNSB-032	1	BE32723_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Cmae_Bot2011_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	ATGAACAGTCTATCCTCCTTTAG	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32723_Hsan_Wil2021_COI	CNSB-032	1	BE32723_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Hsan_Wil2021_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	CCTGGGCCGGTATAGTAGGT	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32723_Mros_Gib2022_COI	CNSB-032	1	BE32723_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Mros_Gib2022_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	ACTTCTACCTCCTGCTCTTCTACTTCTC	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32723_Msal_Bot2012_COI	CNSB-032	1	BE32723_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Msal_Bot2012_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	GYTAGTTCCRATGATGTTAGCTG	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32723_Mstr_Wil2021_COI	CNSB-032	1	BE32723_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Mstr_Wil2021_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	GTAGTGGTAACTACTCATGCTTTA	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32723_Pvir_Dia2013_COI	CNSB-032	1	BE32723_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Pvir_Dia2013_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	CTTAGTGGCATTAATTCGDAATCC	145.78453
BE32723	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.92009	WGS84	BE32723	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32723_Rhar_Sim2018_COI	CNSB-032	1	BE32723_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32723	Sample	BE32723	targeted	Absent	Sample	Rhar_Sim2018_COI	CNSB-032	COI	45	BE32723	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-032	CTGCTGCTATTGCTCATG	145.78453
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32725_Aamu_Bax2006_COI	CNSB-034	1	BE32725_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Aamu_Bax2006_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	GCACAACCGGGATCTTTACTTC	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32725_Pcan_Bot2011_IGS	CNSB-034	1	BE32725_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Pcan_Bot2011_IGS	CNSB-034	IGS	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	CGTAATCCTCAGTACTGGCTA	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32725_Pper_Dia2013_COI	CNSB-034	1	BE32725_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Pper_Dia2013_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	CTTAGTGGCATTAATTCGDAATCC	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32725_Sspa_Oph2007_COI	CNSB-034	1	BE32725_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Sspa_Oph2007_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	GAAGGCCTTCCGATGATC	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32725_Upin_Bot2011_COI	CNSB-034	1	BE32725_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Upin_Bot2011_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	CTTTAATTACAGCGTTTTTATTGTTGT	145.78422
BE32725	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.91725	WGS84	BE32725	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32725_Vgib_Wil2025_COI	CNSB-034	1	BE32725_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32725	Sample	BE32725	targeted	Absent	Sample	Vgib_Wil2025_COI	CNSB-034	COI	45	BE32725	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-034	CCCCTCTTTACAAGTTCTTTAGGGT	145.78422
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32726_Asen_Wil2025_COI	CNSB-035	1	BE32726_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Asen_Wil2025_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	GTTGTTGTTACAACACATGCTCTTTTG	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32726_Cjap_Sim2018_COI	CNSB-035	1	BE32726_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Cjap_Sim2018_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	TTAATATACGGTCATTTGGTATGAGTATAGATC	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32726_Esin_And2018_Cyb	CNSB-035	1	BE32726_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Esin_And2018_Cyb	CNSB-035	Cytb	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	ACCCCTCCTCATATCCAACCA	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32726_Mgig_Bot2012_COI	CNSB-035	1	BE32726_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Mgig_Bot2012_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	TCTTATTCGTTGGAGACTTTATAACCCT	145.78546
BE32726	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-16.9172	WGS84	BE32726	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-CNS_23-24_B	18/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32726_Mjap_Gib2022_COI	CNSB-035	1	BE32726_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32726	Sample	BE32726	targeted	Absent	Sample	Mjap_Gib2022_COI	CNSB-035	COI	45	BE32726	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	CNSB-035	AATTTAATTGTGACTAGGCATGGTCTG	145.78546
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32733_Asen_Wil2025_COI	TSVB-001	1	BE32733_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	GTTGTTGTTACAACACATGCTCTTTTG	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32733_Cjap_Sim2018_COI	TSVB-001	1	BE32733_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32733_Esin_And2018_Cyb	TSVB-001	1	BE32733_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-001	Cytb	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	ACCCCTCCTCATATCCAACCA	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32733_Mgig_Bot2012_COI	TSVB-001	1	BE32733_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	TCTTATTCGTTGGAGACTTTATAACCCT	146.84256
BE32733	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24222	WGS84	BE32733	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32733_Mjap_Gib2022_COI	TSVB-001	1	BE32733_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32733	Sample	BE32733	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-001	COI	45	BE32733	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-001	AATTTAATTGTGACTAGGCATGGTCTG	146.84256
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32734_Cmae_Bot2011_COI	TSVB-002	1	BE32734_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	ATGAACAGTCTATCCTCCTTTAG	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32734_Hsan_Wil2021_COI	TSVB-002	1	BE32734_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	CCTGGGCCGGTATAGTAGGT	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32734_Mros_Gib2022_COI	TSVB-002	1	BE32734_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32734_Msal_Bot2012_COI	TSVB-002	1	BE32734_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	GYTAGTTCCRATGATGTTAGCTG	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32734_Mstr_Wil2021_COI	TSVB-002	1	BE32734_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	GTAGTGGTAACTACTCATGCTTTA	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32734_Pvir_Dia2013_COI	TSVB-002	1	BE32734_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	CTTAGTGGCATTAATTCGDAATCC	146.84133
BE32734	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24259	WGS84	BE32734	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32734_Rhar_Sim2018_COI	TSVB-002	1	BE32734_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32734	Sample	BE32734	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-002	COI	45	BE32734	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-002	CTGCTGCTATTGCTCATG	146.84133
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32736_Aamu_Bax2006_COI	TSVB-004	1	BE32736_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	GCACAACCGGGATCTTTACTTC	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32736_Pcan_Bot2011_IGS	TSVB-004	1	BE32736_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-004	IGS	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	CGTAATCCTCAGTACTGGCTA	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32736_Pper_Dia2013_COI	TSVB-004	1	BE32736_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	CTTAGTGGCATTAATTCGDAATCC	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32736_Sspa_Oph2007_COI	TSVB-004	1	BE32736_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	GAAGGCCTTCCGATGATC	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32736_Upin_Bot2011_COI	TSVB-004	1	BE32736_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	CTTTAATTACAGCGTTTTTATTGTTGT	146.8404
BE32736	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24121	WGS84	BE32736	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32736_Vgib_Wil2025_COI	TSVB-004	1	BE32736_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32736	Sample	BE32736	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-004	COI	45	BE32736	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-004	CCCCTCTTTACAAGTTCTTTAGGGT	146.8404
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32737_Asen_Wil2025_COI	TSVB-005	1	BE32737_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	GTTGTTGTTACAACACATGCTCTTTTG	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32737_Cjap_Sim2018_COI	TSVB-005	1	BE32737_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32737_Esin_And2018_Cyb	TSVB-005	1	BE32737_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-005	Cytb	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	ACCCCTCCTCATATCCAACCA	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32737_Mgig_Bot2012_COI	TSVB-005	1	BE32737_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	TCTTATTCGTTGGAGACTTTATAACCCT	146.84199
BE32737	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24112	WGS84	BE32737	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32737_Mjap_Gib2022_COI	TSVB-005	1	BE32737_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32737	Sample	BE32737	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-005	COI	45	BE32737	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-005	AATTTAATTGTGACTAGGCATGGTCTG	146.84199
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32738_Cmae_Bot2011_COI	TSVB-006	1	BE32738_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	ATGAACAGTCTATCCTCCTTTAG	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32738_Hsan_Wil2021_COI	TSVB-006	1	BE32738_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	CCTGGGCCGGTATAGTAGGT	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32738_Mros_Gib2022_COI	TSVB-006	1	BE32738_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32738_Msal_Bot2012_COI	TSVB-006	1	BE32738_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	GYTAGTTCCRATGATGTTAGCTG	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32738_Mstr_Wil2021_COI	TSVB-006	1	BE32738_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	GTAGTGGTAACTACTCATGCTTTA	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32738_Pvir_Dia2013_COI	TSVB-006	1	BE32738_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	CTTAGTGGCATTAATTCGDAATCC	146.84104
BE32738	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24	WGS84	BE32738	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32738_Rhar_Sim2018_COI	TSVB-006	1	BE32738_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32738	Sample	BE32738	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-006	COI	45	BE32738	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-006	CTGCTGCTATTGCTCATG	146.84104
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32740_Asen_Wil2025_COI	TSVB-008	1	BE32740_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	GTTGTTGTTACAACACATGCTCTTTTG	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32740_Cjap_Sim2018_COI	TSVB-008	1	BE32740_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32740_Esin_And2018_Cyb	TSVB-008	1	BE32740_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-008	Cytb	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	ACCCCTCCTCATATCCAACCA	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32740_Mgig_Bot2012_COI	TSVB-008	1	BE32740_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	TCTTATTCGTTGGAGACTTTATAACCCT	146.83793
BE32740	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24024	WGS84	BE32740	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32740_Mjap_Gib2022_COI	TSVB-008	1	BE32740_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32740	Sample	BE32740	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-008	COI	45	BE32740	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-008	AATTTAATTGTGACTAGGCATGGTCTG	146.83793
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32741_Cmae_Bot2011_COI	TSVB-009	1	BE32741_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	ATGAACAGTCTATCCTCCTTTAG	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32741_Hsan_Wil2021_COI	TSVB-009	1	BE32741_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	CCTGGGCCGGTATAGTAGGT	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32741_Mros_Gib2022_COI	TSVB-009	1	BE32741_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32741_Msal_Bot2012_COI	TSVB-009	1	BE32741_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	GYTAGTTCCRATGATGTTAGCTG	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32741_Mstr_Wil2021_COI	TSVB-009	1	BE32741_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	GTAGTGGTAACTACTCATGCTTTA	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32741_Pvir_Dia2013_COI	TSVB-009	1	BE32741_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	CTTAGTGGCATTAATTCGDAATCC	146.84027
BE32741	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23863	WGS84	BE32741	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32741_Rhar_Sim2018_COI	TSVB-009	1	BE32741_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32741	Sample	BE32741	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-009	COI	45	BE32741	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-009	CTGCTGCTATTGCTCATG	146.84027
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32743_Aamu_Bax2006_COI	TSVB-011	1	BE32743_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	GCACAACCGGGATCTTTACTTC	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32743_Pcan_Bot2011_IGS	TSVB-011	1	BE32743_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-011	IGS	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	CGTAATCCTCAGTACTGGCTA	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32743_Pper_Dia2013_COI	TSVB-011	1	BE32743_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	CTTAGTGGCATTAATTCGDAATCC	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32743_Sspa_Oph2007_COI	TSVB-011	1	BE32743_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	GAAGGCCTTCCGATGATC	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32743_Upin_Bot2011_COI	TSVB-011	1	BE32743_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	CTTTAATTACAGCGTTTTTATTGTTGT	146.83706
BE32743	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23908	WGS84	BE32743	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32743_Vgib_Wil2025_COI	TSVB-011	1	BE32743_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32743	Sample	BE32743	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-011	COI	45	BE32743	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-011	CCCCTCTTTACAAGTTCTTTAGGGT	146.83706
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32744_Asen_Wil2025_COI	TSVB-012	1	BE32744_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	GTTGTTGTTACAACACATGCTCTTTTG	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32744_Cjap_Sim2018_COI	TSVB-012	1	BE32744_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32744_Esin_And2018_Cyb	TSVB-012	1	BE32744_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-012	Cytb	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	ACCCCTCCTCATATCCAACCA	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32744_Mgig_Bot2012_COI	TSVB-012	1	BE32744_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	TCTTATTCGTTGGAGACTTTATAACCCT	146.83539
BE32744	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23917	WGS84	BE32744	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32744_Mjap_Gib2022_COI	TSVB-012	1	BE32744_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32744	Sample	BE32744	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-012	COI	45	BE32744	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-012	AATTTAATTGTGACTAGGCATGGTCTG	146.83539
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32745_Cmae_Bot2011_COI	TSVB-013	1	BE32745_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	ATGAACAGTCTATCCTCCTTTAG	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32745_Hsan_Wil2021_COI	TSVB-013	1	BE32745_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	CCTGGGCCGGTATAGTAGGT	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32745_Mros_Gib2022_COI	TSVB-013	1	BE32745_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32745_Msal_Bot2012_COI	TSVB-013	1	BE32745_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	GYTAGTTCCRATGATGTTAGCTG	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32745_Mstr_Wil2021_COI	TSVB-013	1	BE32745_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	GTAGTGGTAACTACTCATGCTTTA	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32745_Pvir_Dia2013_COI	TSVB-013	1	BE32745_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	CTTAGTGGCATTAATTCGDAATCC	146.83377
BE32745	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23929	WGS84	BE32745	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32745_Rhar_Sim2018_COI	TSVB-013	1	BE32745_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32745	Sample	BE32745	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-013	COI	45	BE32745	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-013	CTGCTGCTATTGCTCATG	146.83377
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32747_Aamu_Bax2006_COI	TSVB-015	1	BE32747_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	GCACAACCGGGATCTTTACTTC	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32747_Pcan_Bot2011_IGS	TSVB-015	1	BE32747_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-015	IGS	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	CGTAATCCTCAGTACTGGCTA	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32747_Pper_Dia2013_COI	TSVB-015	1	BE32747_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	CTTAGTGGCATTAATTCGDAATCC	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32747_Sspa_Oph2007_COI	TSVB-015	1	BE32747_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	GAAGGCCTTCCGATGATC	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32747_Upin_Bot2011_COI	TSVB-015	1	BE32747_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	CTTTAATTACAGCGTTTTTATTGTTGT	146.83915
BE32747	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23745	WGS84	BE32747	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32747_Vgib_Wil2025_COI	TSVB-015	1	BE32747_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32747	Sample	BE32747	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-015	COI	45	BE32747	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-015	CCCCTCTTTACAAGTTCTTTAGGGT	146.83915
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32748_Asen_Wil2025_COI	TSVB-016	1	BE32748_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	GTTGTTGTTACAACACATGCTCTTTTG	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32748_Cjap_Sim2018_COI	TSVB-016	1	BE32748_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32748_Esin_And2018_Cyb	TSVB-016	1	BE32748_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-016	Cytb	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	ACCCCTCCTCATATCCAACCA	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32748_Mgig_Bot2012_COI	TSVB-016	1	BE32748_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	TCTTATTCGTTGGAGACTTTATAACCCT	146.83762
BE32748	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2376	WGS84	BE32748	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32748_Mjap_Gib2022_COI	TSVB-016	1	BE32748_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32748	Sample	BE32748	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-016	COI	45	BE32748	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-016	AATTTAATTGTGACTAGGCATGGTCTG	146.83762
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32749_Cmae_Bot2011_COI	TSVB-017	1	BE32749_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	ATGAACAGTCTATCCTCCTTTAG	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32749_Mros_Gib2022_COI	TSVB-017	1	BE32749_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32749_Msal_Bot2012_COI	TSVB-017	1	BE32749_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	GYTAGTTCCRATGATGTTAGCTG	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32749_Mstr_Wil2021_COI	TSVB-017	1	BE32749_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	GTAGTGGTAACTACTCATGCTTTA	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32749_Pvir_Dia2013_COI	TSVB-017	1	BE32749_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	CTTAGTGGCATTAATTCGDAATCC	146.83614
BE32749	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23771	WGS84	BE32749	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32749_Rhar_Sim2018_COI	TSVB-017	1	BE32749_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32749	Sample	BE32749	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-017	COI	45	BE32749	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-017	CTGCTGCTATTGCTCATG	146.83614
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32750_Aamu_Bax2006_COI	TSVB-018	1	BE32750_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	GCACAACCGGGATCTTTACTTC	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32750_Pcan_Bot2011_IGS	TSVB-018	1	BE32750_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-018	IGS	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	CGTAATCCTCAGTACTGGCTA	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32750_Pper_Dia2013_COI	TSVB-018	1	BE32750_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	CTTAGTGGCATTAATTCGDAATCC	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32750_Sspa_Oph2007_COI	TSVB-018	1	BE32750_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	GAAGGCCTTCCGATGATC	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32750_Upin_Bot2011_COI	TSVB-018	1	BE32750_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	CTTTAATTACAGCGTTTTTATTGTTGT	146.83444
BE32750	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23803	WGS84	BE32750	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32750_Vgib_Wil2025_COI	TSVB-018	1	BE32750_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32750	Sample	BE32750	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-018	COI	45	BE32750	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-018	CCCCTCTTTACAAGTTCTTTAGGGT	146.83444
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32751_Asen_Wil2025_COI	TSVB-019	1	BE32751_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	GTTGTTGTTACAACACATGCTCTTTTG	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32751_Cjap_Sim2018_COI	TSVB-019	1	BE32751_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32751_Esin_And2018_Cyb	TSVB-019	1	BE32751_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-019	Cytb	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	ACCCCTCCTCATATCCAACCA	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32751_Mgig_Bot2012_COI	TSVB-019	1	BE32751_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	TCTTATTCGTTGGAGACTTTATAACCCT	146.83331
BE32751	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23813	WGS84	BE32751	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32751_Mjap_Gib2022_COI	TSVB-019	1	BE32751_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32751	Sample	BE32751	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-019	COI	45	BE32751	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-019	AATTTAATTGTGACTAGGCATGGTCTG	146.83331
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32752_Cmae_Bot2011_COI	TSVB-020	1	BE32752_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	ATGAACAGTCTATCCTCCTTTAG	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32752_Hsan_Wil2021_COI	TSVB-020	1	BE32752_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	CCTGGGCCGGTATAGTAGGT	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32752_Mros_Gib2022_COI	TSVB-020	1	BE32752_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32752_Msal_Bot2012_COI	TSVB-020	1	BE32752_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	GYTAGTTCCRATGATGTTAGCTG	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32752_Mstr_Wil2021_COI	TSVB-020	1	BE32752_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	GTAGTGGTAACTACTCATGCTTTA	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32752_Pvir_Dia2013_COI	TSVB-020	1	BE32752_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	CTTAGTGGCATTAATTCGDAATCC	146.83792
BE32752	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.23666	WGS84	BE32752	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32752_Rhar_Sim2018_COI	TSVB-020	1	BE32752_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32752	Sample	BE32752	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-020	COI	45	BE32752	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-020	CTGCTGCTATTGCTCATG	146.83792
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32754_Aamu_Bax2006_COI	TSVB-022	1	BE32754_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	GCACAACCGGGATCTTTACTTC	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32754_Pcan_Bot2011_IGS	TSVB-022	1	BE32754_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-022	IGS	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	CGTAATCCTCAGTACTGGCTA	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32754_Pper_Dia2013_COI	TSVB-022	1	BE32754_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	CTTAGTGGCATTAATTCGDAATCC	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32754_Sspa_Oph2007_COI	TSVB-022	1	BE32754_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	GAAGGCCTTCCGATGATC	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32754_Upin_Bot2011_COI	TSVB-022	1	BE32754_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	CTTTAATTACAGCGTTTTTATTGTTGT	146.83487
BE32754	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2371	WGS84	BE32754	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32754_Vgib_Wil2025_COI	TSVB-022	1	BE32754_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32754	Sample	BE32754	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-022	COI	45	BE32754	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-022	CCCCTCTTTACAAGTTCTTTAGGGT	146.83487
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32755_Asen_Wil2025_COI	TSVB-023	1	BE32755_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	GTTGTTGTTACAACACATGCTCTTTTG	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32755_Cjap_Sim2018_COI	TSVB-023	1	BE32755_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32755_Esin_And2018_Cyb	TSVB-023	1	BE32755_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-023	Cytb	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	ACCCCTCCTCATATCCAACCA	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32755_Mgig_Bot2012_COI	TSVB-023	1	BE32755_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	TCTTATTCGTTGGAGACTTTATAACCCT	146.83892
BE32755	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24134	WGS84	BE32755	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32755_Mjap_Gib2022_COI	TSVB-023	1	BE32755_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32755	Sample	BE32755	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-023	COI	45	BE32755	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-023	AATTTAATTGTGACTAGGCATGGTCTG	146.83892
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32756_Cmae_Bot2011_COI	TSVB-024	1	BE32756_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	ATGAACAGTCTATCCTCCTTTAG	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32756_Hsan_Wil2021_COI	TSVB-024	1	BE32756_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	CCTGGGCCGGTATAGTAGGT	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32756_Mros_Gib2022_COI	TSVB-024	1	BE32756_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32756_Msal_Bot2012_COI	TSVB-024	1	BE32756_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	GYTAGTTCCRATGATGTTAGCTG	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32756_Mstr_Wil2021_COI	TSVB-024	1	BE32756_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	GTAGTGGTAACTACTCATGCTTTA	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32756_Pvir_Dia2013_COI	TSVB-024	1	BE32756_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	CTTAGTGGCATTAATTCGDAATCC	146.83443
BE32756	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.2445	WGS84	BE32756	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32756_Rhar_Sim2018_COI	TSVB-024	1	BE32756_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32756	Sample	BE32756	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-024	COI	45	BE32756	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-024	CTGCTGCTATTGCTCATG	146.83443
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32758_Aamu_Bax2006_COI	TSVB-026	1	BE32758_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	GCACAACCGGGATCTTTACTTC	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32758_Pcan_Bot2011_IGS	TSVB-026	1	BE32758_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-026	IGS	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	CGTAATCCTCAGTACTGGCTA	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32758_Pper_Dia2013_COI	TSVB-026	1	BE32758_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	CTTAGTGGCATTAATTCGDAATCC	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32758_Sspa_Oph2007_COI	TSVB-026	1	BE32758_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	GAAGGCCTTCCGATGATC	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32758_Upin_Bot2011_COI	TSVB-026	1	BE32758_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	CTTTAATTACAGCGTTTTTATTGTTGT	146.83467
BE32758	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24627	WGS84	BE32758	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32758_Vgib_Wil2025_COI	TSVB-026	1	BE32758_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32758	Sample	BE32758	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-026	COI	45	BE32758	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-026	CCCCTCTTTACAAGTTCTTTAGGGT	146.83467
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32759_Asen_Wil2025_COI	TSVB-027	1	BE32759_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	GTTGTTGTTACAACACATGCTCTTTTG	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32759_Cjap_Sim2018_COI	TSVB-027	1	BE32759_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32759_Esin_And2018_Cyb	TSVB-027	1	BE32759_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-027	Cytb	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	ACCCCTCCTCATATCCAACCA	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32759_Mgig_Bot2012_COI	TSVB-027	1	BE32759_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	TCTTATTCGTTGGAGACTTTATAACCCT	146.83533
BE32759	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24604	WGS84	BE32759	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32759_Mjap_Gib2022_COI	TSVB-027	1	BE32759_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32759	Sample	BE32759	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-027	COI	45	BE32759	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-027	AATTTAATTGTGACTAGGCATGGTCTG	146.83533
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32761_Aamu_Bax2006_COI	TSVB-029	1	BE32761_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	GCACAACCGGGATCTTTACTTC	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32761_Pcan_Bot2011_IGS	TSVB-029	1	BE32761_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-029	IGS	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	CGTAATCCTCAGTACTGGCTA	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32761_Pper_Dia2013_COI	TSVB-029	1	BE32761_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	CTTAGTGGCATTAATTCGDAATCC	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32761_Sspa_Oph2007_COI	TSVB-029	1	BE32761_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	GAAGGCCTTCCGATGATC	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32761_Upin_Bot2011_COI	TSVB-029	1	BE32761_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	CTTTAATTACAGCGTTTTTATTGTTGT	146.83352
BE32761	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24798	WGS84	BE32761	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32761_Vgib_Wil2025_COI	TSVB-029	1	BE32761_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32761	Sample	BE32761	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-029	COI	45	BE32761	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-029	CCCCTCTTTACAAGTTCTTTAGGGT	146.83352
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32762_Asen_Wil2025_COI	TSVB-030	1	BE32762_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	GTTGTTGTTACAACACATGCTCTTTTG	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32762_Cjap_Sim2018_COI	TSVB-030	1	BE32762_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32762_Esin_And2018_Cyb	TSVB-030	1	BE32762_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-030	Cytb	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	ACCCCTCCTCATATCCAACCA	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32762_Mgig_Bot2012_COI	TSVB-030	1	BE32762_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	TCTTATTCGTTGGAGACTTTATAACCCT	146.83326
BE32762	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24916	WGS84	BE32762	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32762_Mjap_Gib2022_COI	TSVB-030	1	BE32762_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32762	Sample	BE32762	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-030	COI	45	BE32762	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-030	AATTTAATTGTGACTAGGCATGGTCTG	146.83326
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32763_Cmae_Bot2011_COI	TSVB-031	1	BE32763_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	ATGAACAGTCTATCCTCCTTTAG	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32763_Hsan_Wil2021_COI	TSVB-031	1	BE32763_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	CCTGGGCCGGTATAGTAGGT	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32763_Mros_Gib2022_COI	TSVB-031	1	BE32763_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32763_Msal_Bot2012_COI	TSVB-031	1	BE32763_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	GYTAGTTCCRATGATGTTAGCTG	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32763_Mstr_Wil2021_COI	TSVB-031	1	BE32763_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	GTAGTGGTAACTACTCATGCTTTA	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32763_Pvir_Dia2013_COI	TSVB-031	1	BE32763_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	CTTAGTGGCATTAATTCGDAATCC	146.83387
BE32763	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.25051	WGS84	BE32763	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32763_Rhar_Sim2018_COI	TSVB-031	1	BE32763_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32763	Sample	BE32763	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-031	COI	45	BE32763	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-031	CTGCTGCTATTGCTCATG	146.83387
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32765_Aamu_Bax2006_COI	TSVB-033	1	BE32765_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Aamu_Bax2006_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	GCACAACCGGGATCTTTACTTC	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32765_Pcan_Bot2011_IGS	TSVB-033	1	BE32765_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Pcan_Bot2011_IGS	TSVB-033	IGS	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	CGTAATCCTCAGTACTGGCTA	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32765_Pper_Dia2013_COI	TSVB-033	1	BE32765_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Pper_Dia2013_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	CTTAGTGGCATTAATTCGDAATCC	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32765_Sspa_Oph2007_COI	TSVB-033	1	BE32765_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Sspa_Oph2007_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	GAAGGCCTTCCGATGATC	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32765_Upin_Bot2011_COI	TSVB-033	1	BE32765_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Upin_Bot2011_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	CTTTAATTACAGCGTTTTTATTGTTGT	146.83413
BE32765	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24814	WGS84	BE32765	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32765_Vgib_Wil2025_COI	TSVB-033	1	BE32765_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32765	Sample	BE32765	targeted	Absent	Sample	Vgib_Wil2025_COI	TSVB-033	COI	45	BE32765	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-033	CCCCTCTTTACAAGTTCTTTAGGGT	146.83413
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32766_Asen_Wil2025_COI	TSVB-034	1	BE32766_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Asen_Wil2025_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	GTTGTTGTTACAACACATGCTCTTTTG	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32766_Cjap_Sim2018_COI	TSVB-034	1	BE32766_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Cjap_Sim2018_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32766_Esin_And2018_Cyb	TSVB-034	1	BE32766_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Esin_And2018_Cyb	TSVB-034	Cytb	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	ACCCCTCCTCATATCCAACCA	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32766_Mgig_Bot2012_COI	TSVB-034	1	BE32766_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Mgig_Bot2012_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	TCTTATTCGTTGGAGACTTTATAACCCT	146.83207
BE32766	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24646	WGS84	BE32766	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32766_Mjap_Gib2022_COI	TSVB-034	1	BE32766_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32766	Sample	BE32766	targeted	Absent	Sample	Mjap_Gib2022_COI	TSVB-034	COI	45	BE32766	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-034	AATTTAATTGTGACTAGGCATGGTCTG	146.83207
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32767_Cmae_Bot2011_COI	TSVB-035	1	BE32767_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Cmae_Bot2011_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	ATGAACAGTCTATCCTCCTTTAG	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32767_Hsan_Wil2021_COI	TSVB-035	1	BE32767_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Hsan_Wil2021_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	CCTGGGCCGGTATAGTAGGT	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32767_Mros_Gib2022_COI	TSVB-035	1	BE32767_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Mros_Gib2022_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32767_Msal_Bot2012_COI	TSVB-035	1	BE32767_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Msal_Bot2012_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	GYTAGTTCCRATGATGTTAGCTG	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32767_Mstr_Wil2021_COI	TSVB-035	1	BE32767_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Mstr_Wil2021_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	GTAGTGGTAACTACTCATGCTTTA	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32767_Pvir_Dia2013_COI	TSVB-035	1	BE32767_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Pvir_Dia2013_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	CTTAGTGGCATTAATTCGDAATCC	146.83158
BE32767	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16426	-19.24815	WGS84	BE32767	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-TSV_23-24_B	23/03/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32767_Rhar_Sim2018_COI	TSVB-035	1	BE32767_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32767	Sample	BE32767	targeted	Absent	Sample	Rhar_Sim2018_COI	TSVB-035	COI	45	BE32767	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	TSVB-035	CTGCTGCTATTGCTCATG	146.83158
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32774_Cmae_Bot2011_COI	DRWB-001	1	BE32774_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	ATGAACAGTCTATCCTCCTTTAG	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32774_Hsan_Wil2021_COI	DRWB-001	1	BE32774_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	CCTGGGCCGGTATAGTAGGT	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32774_Mros_Gib2022_COI	DRWB-001	1	BE32774_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32774_Msal_Bot2012_COI	DRWB-001	1	BE32774_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	GYTAGTTCCRATGATGTTAGCTG	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32774_Mstr_Wil2021_COI	DRWB-001	1	BE32774_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	GTAGTGGTAACTACTCATGCTTTA	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32774_Pvir_Dia2013_COI	DRWB-001	1	BE32774_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	CTTAGTGGCATTAATTCGDAATCC	130.90592
BE32774	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51353	WGS84	BE32774	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32774_Rhar_Sim2018_COI	DRWB-001	1	BE32774_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32774	Sample	BE32774	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-001	COI	45	BE32774	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-001	CTGCTGCTATTGCTCATG	130.90592
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32776_Aamu_Bax2006_COI	DRWB-003	1	BE32776_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	GCACAACCGGGATCTTTACTTC	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32776_Pcan_Bot2011_IGS	DRWB-003	1	BE32776_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-003	IGS	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	CGTAATCCTCAGTACTGGCTA	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32776_Pper_Dia2013_COI	DRWB-003	1	BE32776_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	CTTAGTGGCATTAATTCGDAATCC	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32776_Sspa_Oph2007_COI	DRWB-003	1	BE32776_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	GAAGGCCTTCCGATGATC	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32776_Upin_Bot2011_COI	DRWB-003	1	BE32776_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	CTTTAATTACAGCGTTTTTATTGTTGT	130.90357
BE32776	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51152	WGS84	BE32776	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32776_Vgib_Wil2025_COI	DRWB-003	1	BE32776_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32776	Sample	BE32776	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-003	COI	45	BE32776	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-003	CCCCTCTTTACAAGTTCTTTAGGGT	130.90357
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32777_Asen_Wil2025_COI	DRWB-004	1	BE32777_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	GTTGTTGTTACAACACATGCTCTTTTG	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32777_Cjap_Sim2018_COI	DRWB-004	1	BE32777_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32777_Esin_And2018_Cyb	DRWB-004	1	BE32777_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-004	Cytb	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	ACCCCTCCTCATATCCAACCA	130.9043
BE32777	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51053	WGS84	BE32777	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32777_Mjap_Gib2022_COI	DRWB-004	1	BE32777_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32777	Sample	BE32777	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-004	COI	45	BE32777	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-004	AATTTAATTGTGACTAGGCATGGTCTG	130.9043
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32778_Cmae_Bot2011_COI	DRWB-005	1	BE32778_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	ATGAACAGTCTATCCTCCTTTAG	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32778_Hsan_Wil2021_COI	DRWB-005	1	BE32778_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	CCTGGGCCGGTATAGTAGGT	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32778_Mros_Gib2022_COI	DRWB-005	1	BE32778_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32778_Msal_Bot2012_COI	DRWB-005	1	BE32778_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	GYTAGTTCCRATGATGTTAGCTG	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32778_Mstr_Wil2021_COI	DRWB-005	1	BE32778_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	GTAGTGGTAACTACTCATGCTTTA	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32778_Pvir_Dia2013_COI	DRWB-005	1	BE32778_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	CTTAGTGGCATTAATTCGDAATCC	130.90546
BE32778	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51061	WGS84	BE32778	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32778_Rhar_Sim2018_COI	DRWB-005	1	BE32778_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32778	Sample	BE32778	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-005	COI	45	BE32778	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-005	CTGCTGCTATTGCTCATG	130.90546
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32780_Asen_Wil2025_COI	DRWB-007	1	BE32780_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	GTTGTTGTTACAACACATGCTCTTTTG	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32780_Cjap_Sim2018_COI	DRWB-007	1	BE32780_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32780_Esin_And2018_Cyb	DRWB-007	1	BE32780_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-007	Cytb	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	ACCCCTCCTCATATCCAACCA	130.90486
BE32780	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50958	WGS84	BE32780	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32780_Mjap_Gib2022_COI	DRWB-007	1	BE32780_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32780	Sample	BE32780	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-007	COI	45	BE32780	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-007	AATTTAATTGTGACTAGGCATGGTCTG	130.90486
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32781_Cmae_Bot2011_COI	DRWB-008	1	BE32781_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	ATGAACAGTCTATCCTCCTTTAG	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32781_Hsan_Wil2021_COI	DRWB-008	1	BE32781_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	CCTGGGCCGGTATAGTAGGT	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32781_Mros_Gib2022_COI	DRWB-008	1	BE32781_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32781_Msal_Bot2012_COI	DRWB-008	1	BE32781_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	GYTAGTTCCRATGATGTTAGCTG	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32781_Mstr_Wil2021_COI	DRWB-008	1	BE32781_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	GTAGTGGTAACTACTCATGCTTTA	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32781_Pvir_Dia2013_COI	DRWB-008	1	BE32781_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	CTTAGTGGCATTAATTCGDAATCC	130.9043
BE32781	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50856	WGS84	BE32781	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32781_Rhar_Sim2018_COI	DRWB-008	1	BE32781_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32781	Sample	BE32781	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-008	COI	45	BE32781	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-008	CTGCTGCTATTGCTCATG	130.9043
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32783_Aamu_Bax2006_COI	DRWB-010	1	BE32783_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	GCACAACCGGGATCTTTACTTC	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32783_Pcan_Bot2011_IGS	DRWB-010	1	BE32783_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-010	IGS	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	CGTAATCCTCAGTACTGGCTA	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32783_Pper_Dia2013_COI	DRWB-010	1	BE32783_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	CTTAGTGGCATTAATTCGDAATCC	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32783_Sspa_Oph2007_COI	DRWB-010	1	BE32783_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	GAAGGCCTTCCGATGATC	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32783_Upin_Bot2011_COI	DRWB-010	1	BE32783_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	CTTTAATTACAGCGTTTTTATTGTTGT	130.90673
BE32783	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50857	WGS84	BE32783	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32783_Vgib_Wil2025_COI	DRWB-010	1	BE32783_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32783	Sample	BE32783	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-010	COI	45	BE32783	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-010	CCCCTCTTTACAAGTTCTTTAGGGT	130.90673
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32784_Asen_Wil2025_COI	DRWB-011	1	BE32784_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	GTTGTTGTTACAACACATGCTCTTTTG	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32784_Cjap_Sim2018_COI	DRWB-011	1	BE32784_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32784_Esin_And2018_Cyb	DRWB-011	1	BE32784_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-011	Cytb	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	ACCCCTCCTCATATCCAACCA	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32784_Mgig_Bot2012_COI	DRWB-011	1	BE32784_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	TCTTATTCGTTGGAGACTTTATAACCCT	130.90786
BE32784	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50863	WGS84	BE32784	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32784_Mjap_Gib2022_COI	DRWB-011	1	BE32784_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32784	Sample	BE32784	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-011	COI	45	BE32784	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-011	AATTTAATTGTGACTAGGCATGGTCTG	130.90786
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32785_Cmae_Bot2011_COI	DRWB-012	1	BE32785_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	ATGAACAGTCTATCCTCCTTTAG	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32785_Hsan_Wil2021_COI	DRWB-012	1	BE32785_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	CCTGGGCCGGTATAGTAGGT	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32785_Mros_Gib2022_COI	DRWB-012	1	BE32785_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32785_Msal_Bot2012_COI	DRWB-012	1	BE32785_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	GYTAGTTCCRATGATGTTAGCTG	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32785_Mstr_Wil2021_COI	DRWB-012	1	BE32785_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	GTAGTGGTAACTACTCATGCTTTA	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32785_Pvir_Dia2013_COI	DRWB-012	1	BE32785_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	CTTAGTGGCATTAATTCGDAATCC	130.90373
BE32785	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50758	WGS84	BE32785	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32785_Rhar_Sim2018_COI	DRWB-012	1	BE32785_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32785	Sample	BE32785	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-012	COI	45	BE32785	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-012	CTGCTGCTATTGCTCATG	130.90373
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32787_Aamu_Bax2006_COI	DRWB-014	1	BE32787_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	GCACAACCGGGATCTTTACTTC	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32787_Pcan_Bot2011_IGS	DRWB-014	1	BE32787_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-014	IGS	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	CGTAATCCTCAGTACTGGCTA	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32787_Pper_Dia2013_COI	DRWB-014	1	BE32787_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	CTTAGTGGCATTAATTCGDAATCC	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32787_Sspa_Oph2007_COI	DRWB-014	1	BE32787_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	GAAGGCCTTCCGATGATC	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32787_Upin_Bot2011_COI	DRWB-014	1	BE32787_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	CTTTAATTACAGCGTTTTTATTGTTGT	130.90613
BE32787	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32787	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32787_Vgib_Wil2025_COI	DRWB-014	1	BE32787_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32787	Sample	BE32787	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-014	COI	45	BE32787	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-014	CCCCTCTTTACAAGTTCTTTAGGGT	130.90613
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32788_Asen_Wil2025_COI	DRWB-015	1	BE32788_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	GTTGTTGTTACAACACATGCTCTTTTG	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32788_Cjap_Sim2018_COI	DRWB-015	1	BE32788_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32788_Esin_And2018_Cyb	DRWB-015	1	BE32788_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-015	Cytb	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	ACCCCTCCTCATATCCAACCA	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32788_Mgig_Bot2012_COI	DRWB-015	1	BE32788_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	TCTTATTCGTTGGAGACTTTATAACCCT	130.90725
BE32788	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50762	WGS84	BE32788	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32788_Mjap_Gib2022_COI	DRWB-015	1	BE32788_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32788	Sample	BE32788	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-015	COI	45	BE32788	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-015	AATTTAATTGTGACTAGGCATGGTCTG	130.90725
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32789_Cmae_Bot2011_COI	DRWB-016	1	BE32789_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	ATGAACAGTCTATCCTCCTTTAG	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32789_Hsan_Wil2021_COI	DRWB-016	1	BE32789_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	CCTGGGCCGGTATAGTAGGT	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32789_Mros_Gib2022_COI	DRWB-016	1	BE32789_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32789_Msal_Bot2012_COI	DRWB-016	1	BE32789_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	GYTAGTTCCRATGATGTTAGCTG	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32789_Mstr_Wil2021_COI	DRWB-016	1	BE32789_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	GTAGTGGTAACTACTCATGCTTTA	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32789_Pvir_Dia2013_COI	DRWB-016	1	BE32789_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	CTTAGTGGCATTAATTCGDAATCC	130.90842
BE32789	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50766	WGS84	BE32789	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32789_Rhar_Sim2018_COI	DRWB-016	1	BE32789_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32789	Sample	BE32789	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-016	COI	45	BE32789	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-016	CTGCTGCTATTGCTCATG	130.90842
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32790_Aamu_Bax2006_COI	DRWB-017	1	BE32790_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	GCACAACCGGGATCTTTACTTC	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32790_Pcan_Bot2011_IGS	DRWB-017	1	BE32790_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-017	IGS	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	CGTAATCCTCAGTACTGGCTA	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32790_Pper_Dia2013_COI	DRWB-017	1	BE32790_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	CTTAGTGGCATTAATTCGDAATCC	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32790_Sspa_Oph2007_COI	DRWB-017	1	BE32790_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	GAAGGCCTTCCGATGATC	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32790_Upin_Bot2011_COI	DRWB-017	1	BE32790_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	CTTTAATTACAGCGTTTTTATTGTTGT	130.90959
BE32790	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50767	WGS84	BE32790	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32790_Vgib_Wil2025_COI	DRWB-017	1	BE32790_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32790	Sample	BE32790	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-017	COI	45	BE32790	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-017	CCCCTCTTTACAAGTTCTTTAGGGT	130.90959
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32791_Asen_Wil2025_COI	DRWB-018	1	BE32791_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	GTTGTTGTTACAACACATGCTCTTTTG	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32791_Cjap_Sim2018_COI	DRWB-018	1	BE32791_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32791_Esin_And2018_Cyb	DRWB-018	1	BE32791_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-018	Cytb	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	ACCCCTCCTCATATCCAACCA	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32791_Mgig_Bot2012_COI	DRWB-018	1	BE32791_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	TCTTATTCGTTGGAGACTTTATAACCCT	130.91082
BE32791	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5077	WGS84	BE32791	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32791_Mjap_Gib2022_COI	DRWB-018	1	BE32791_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32791	Sample	BE32791	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-018	COI	45	BE32791	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-018	AATTTAATTGTGACTAGGCATGGTCTG	130.91082
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32792_Cmae_Bot2011_COI	DRWB-019	1	BE32792_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	ATGAACAGTCTATCCTCCTTTAG	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32792_Hsan_Wil2021_COI	DRWB-019	1	BE32792_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	CCTGGGCCGGTATAGTAGGT	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32792_Mros_Gib2022_COI	DRWB-019	1	BE32792_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32792_Msal_Bot2012_COI	DRWB-019	1	BE32792_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	GYTAGTTCCRATGATGTTAGCTG	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32792_Mstr_Wil2021_COI	DRWB-019	1	BE32792_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	GTAGTGGTAACTACTCATGCTTTA	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32792_Pvir_Dia2013_COI	DRWB-019	1	BE32792_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	CTTAGTGGCATTAATTCGDAATCC	130.91205
BE32792	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50768	WGS84	BE32792	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32792_Rhar_Sim2018_COI	DRWB-019	1	BE32792_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32792	Sample	BE32792	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-019	COI	45	BE32792	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-019	CTGCTGCTATTGCTCATG	130.91205
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32794_Aamu_Bax2006_COI	DRWB-021	1	BE32794_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	GCACAACCGGGATCTTTACTTC	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32794_Pcan_Bot2011_IGS	DRWB-021	1	BE32794_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-021	IGS	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	CGTAATCCTCAGTACTGGCTA	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32794_Pper_Dia2013_COI	DRWB-021	1	BE32794_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	CTTAGTGGCATTAATTCGDAATCC	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32794_Sspa_Oph2007_COI	DRWB-021	1	BE32794_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	GAAGGCCTTCCGATGATC	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32794_Upin_Bot2011_COI	DRWB-021	1	BE32794_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	CTTTAATTACAGCGTTTTTATTGTTGT	130.90562
BE32794	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50651	WGS84	BE32794	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32794_Vgib_Wil2025_COI	DRWB-021	1	BE32794_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32794	Sample	BE32794	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-021	COI	45	BE32794	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-021	CCCCTCTTTACAAGTTCTTTAGGGT	130.90562
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32795_Asen_Wil2025_COI	DRWB-022	1	BE32795_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	GTTGTTGTTACAACACATGCTCTTTTG	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32795_Cjap_Sim2018_COI	DRWB-022	1	BE32795_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32795_Esin_And2018_Cyb	DRWB-022	1	BE32795_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-022	Cytb	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	ACCCCTCCTCATATCCAACCA	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32795_Mgig_Bot2012_COI	DRWB-022	1	BE32795_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	TCTTATTCGTTGGAGACTTTATAACCCT	130.90676
BE32795	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50658	WGS84	BE32795	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32795_Mjap_Gib2022_COI	DRWB-022	1	BE32795_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32795	Sample	BE32795	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-022	COI	45	BE32795	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-022	AATTTAATTGTGACTAGGCATGGTCTG	130.90676
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32796_Cmae_Bot2011_COI	DRWB-023	1	BE32796_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	ATGAACAGTCTATCCTCCTTTAG	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32796_Hsan_Wil2021_COI	DRWB-023	1	BE32796_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	CCTGGGCCGGTATAGTAGGT	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32796_Mros_Gib2022_COI	DRWB-023	1	BE32796_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32796_Msal_Bot2012_COI	DRWB-023	1	BE32796_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	GYTAGTTCCRATGATGTTAGCTG	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32796_Mstr_Wil2021_COI	DRWB-023	1	BE32796_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	GTAGTGGTAACTACTCATGCTTTA	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32796_Pvir_Dia2013_COI	DRWB-023	1	BE32796_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	CTTAGTGGCATTAATTCGDAATCC	130.90799
BE32796	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50652	WGS84	BE32796	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32796_Rhar_Sim2018_COI	DRWB-023	1	BE32796_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32796	Sample	BE32796	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-023	COI	45	BE32796	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-023	CTGCTGCTATTGCTCATG	130.90799
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32798_Aamu_Bax2006_COI	DRWB-025	1	BE32798_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	GCACAACCGGGATCTTTACTTC	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32798_Pcan_Bot2011_IGS	DRWB-025	1	BE32798_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-025	IGS	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	CGTAATCCTCAGTACTGGCTA	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32798_Pper_Dia2013_COI	DRWB-025	1	BE32798_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	CTTAGTGGCATTAATTCGDAATCC	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32798_Sspa_Oph2007_COI	DRWB-025	1	BE32798_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	GAAGGCCTTCCGATGATC	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32798_Upin_Bot2011_COI	DRWB-025	1	BE32798_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	CTTTAATTACAGCGTTTTTATTGTTGT	130.91038
BE32798	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50664	WGS84	BE32798	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32798_Vgib_Wil2025_COI	DRWB-025	1	BE32798_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32798	Sample	BE32798	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-025	COI	45	BE32798	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-025	CCCCTCTTTACAAGTTCTTTAGGGT	130.91038
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32799_Asen_Wil2025_COI	DRWB-026	1	BE32799_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	GTTGTTGTTACAACACATGCTCTTTTG	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32799_Cjap_Sim2018_COI	DRWB-026	1	BE32799_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32799_Esin_And2018_Cyb	DRWB-026	1	BE32799_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-026	Cytb	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	ACCCCTCCTCATATCCAACCA	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32799_Mgig_Bot2012_COI	DRWB-026	1	BE32799_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	TCTTATTCGTTGGAGACTTTATAACCCT	130.91155
BE32799	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50659	WGS84	BE32799	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32799_Mjap_Gib2022_COI	DRWB-026	1	BE32799_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32799	Sample	BE32799	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-026	COI	45	BE32799	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-026	AATTTAATTGTGACTAGGCATGGTCTG	130.91155
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32800_Cmae_Bot2011_COI	DRWB-027	1	BE32800_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	ATGAACAGTCTATCCTCCTTTAG	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32800_Hsan_Wil2021_COI	DRWB-027	1	BE32800_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	CCTGGGCCGGTATAGTAGGT	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32800_Mros_Gib2022_COI	DRWB-027	1	BE32800_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32800_Msal_Bot2012_COI	DRWB-027	1	BE32800_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	GYTAGTTCCRATGATGTTAGCTG	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32800_Mstr_Wil2021_COI	DRWB-027	1	BE32800_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	GTAGTGGTAACTACTCATGCTTTA	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32800_Pvir_Dia2013_COI	DRWB-027	1	BE32800_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	CTTAGTGGCATTAATTCGDAATCC	130.91277
BE32800	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50666	WGS84	BE32800	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32800_Rhar_Sim2018_COI	DRWB-027	1	BE32800_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32800	Sample	BE32800	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-027	COI	45	BE32800	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-027	CTGCTGCTATTGCTCATG	130.91277
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32802_Aamu_Bax2006_COI	DRWB-029	1	BE32802_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	GCACAACCGGGATCTTTACTTC	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32802_Pcan_Bot2011_IGS	DRWB-029	1	BE32802_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-029	IGS	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	CGTAATCCTCAGTACTGGCTA	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32802_Pper_Dia2013_COI	DRWB-029	1	BE32802_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	CTTAGTGGCATTAATTCGDAATCC	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32802_Sspa_Oph2007_COI	DRWB-029	1	BE32802_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	GAAGGCCTTCCGATGATC	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32802_Upin_Bot2011_COI	DRWB-029	1	BE32802_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	CTTTAATTACAGCGTTTTTATTGTTGT	130.91506
BE32802	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50681	WGS84	BE32802	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32802_Vgib_Wil2025_COI	DRWB-029	1	BE32802_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32802	Sample	BE32802	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-029	COI	45	BE32802	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-029	CCCCTCTTTACAAGTTCTTTAGGGT	130.91506
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32803_Asen_Wil2025_COI	DRWB-030	1	BE32803_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	GTTGTTGTTACAACACATGCTCTTTTG	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32803_Cjap_Sim2018_COI	DRWB-030	1	BE32803_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32803_Esin_And2018_Cyb	DRWB-030	1	BE32803_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-030	Cytb	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	ACCCCTCCTCATATCCAACCA	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32803_Mgig_Bot2012_COI	DRWB-030	1	BE32803_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	TCTTATTCGTTGGAGACTTTATAACCCT	130.90862
BE32803	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32803	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32803_Mjap_Gib2022_COI	DRWB-030	1	BE32803_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32803	Sample	BE32803	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-030	COI	45	BE32803	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-030	AATTTAATTGTGACTAGGCATGGTCTG	130.90862
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32804_Cmae_Bot2011_COI	DRWB-031	1	BE32804_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	ATGAACAGTCTATCCTCCTTTAG	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32804_Hsan_Wil2021_COI	DRWB-031	1	BE32804_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	CCTGGGCCGGTATAGTAGGT	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32804_Mros_Gib2022_COI	DRWB-031	1	BE32804_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32804_Msal_Bot2012_COI	DRWB-031	1	BE32804_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	GYTAGTTCCRATGATGTTAGCTG	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32804_Mstr_Wil2021_COI	DRWB-031	1	BE32804_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	GTAGTGGTAACTACTCATGCTTTA	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32804_Pvir_Dia2013_COI	DRWB-031	1	BE32804_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	CTTAGTGGCATTAATTCGDAATCC	130.90982
BE32804	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50556	WGS84	BE32804	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32804_Rhar_Sim2018_COI	DRWB-031	1	BE32804_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32804	Sample	BE32804	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-031	COI	45	BE32804	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-031	CTGCTGCTATTGCTCATG	130.90982
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32806_Aamu_Bax2006_COI	DRWB-033	1	BE32806_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	GCACAACCGGGATCTTTACTTC	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32806_Pcan_Bot2011_IGS	DRWB-033	1	BE32806_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-033	IGS	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	CGTAATCCTCAGTACTGGCTA	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32806_Pper_Dia2013_COI	DRWB-033	1	BE32806_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	CTTAGTGGCATTAATTCGDAATCC	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32806_Sspa_Oph2007_COI	DRWB-033	1	BE32806_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	GAAGGCCTTCCGATGATC	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32806_Upin_Bot2011_COI	DRWB-033	1	BE32806_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	CTTTAATTACAGCGTTTTTATTGTTGT	130.91223
BE32806	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50563	WGS84	BE32806	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32806_Vgib_Wil2025_COI	DRWB-033	1	BE32806_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32806	Sample	BE32806	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-033	COI	45	BE32806	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-033	CCCCTCTTTACAAGTTCTTTAGGGT	130.91223
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32807_Asen_Wil2025_COI	DRWB-034	1	BE32807_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	GTTGTTGTTACAACACATGCTCTTTTG	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32807_Cjap_Sim2018_COI	DRWB-034	1	BE32807_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32807_Esin_And2018_Cyb	DRWB-034	1	BE32807_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-034	Cytb	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	ACCCCTCCTCATATCCAACCA	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32807_Mgig_Bot2012_COI	DRWB-034	1	BE32807_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	TCTTATTCGTTGGAGACTTTATAACCCT	130.91346
BE32807	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50561	WGS84	BE32807	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32807_Mjap_Gib2022_COI	DRWB-034	1	BE32807_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32807	Sample	BE32807	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-034	COI	45	BE32807	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-034	AATTTAATTGTGACTAGGCATGGTCTG	130.91346
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32808_Cmae_Bot2011_COI	DRWB-035	1	BE32808_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	ATGAACAGTCTATCCTCCTTTAG	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32808_Hsan_Wil2021_COI	DRWB-035	1	BE32808_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	CCTGGGCCGGTATAGTAGGT	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32808_Mros_Gib2022_COI	DRWB-035	1	BE32808_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32808_Msal_Bot2012_COI	DRWB-035	1	BE32808_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	GYTAGTTCCRATGATGTTAGCTG	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32808_Mstr_Wil2021_COI	DRWB-035	1	BE32808_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	GTAGTGGTAACTACTCATGCTTTA	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32808_Pvir_Dia2013_COI	DRWB-035	1	BE32808_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	CTTAGTGGCATTAATTCGDAATCC	130.91296
BE32808	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.50462	WGS84	BE32808	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW02_23-24_B	3/04/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32808_Rhar_Sim2018_COI	DRWB-035	1	BE32808_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32808	Sample	BE32808	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-035	COI	45	BE32808	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-035	CTGCTGCTATTGCTCATG	130.91296
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32815_Cmae_Bot2011_COI	DRWB-036	1	BE32815_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	ATGAACAGTCTATCCTCCTTTAG	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32815_Hsan_Wil2021_COI	DRWB-036	1	BE32815_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	CCTGGGCCGGTATAGTAGGT	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32815_Mros_Gib2022_COI	DRWB-036	1	BE32815_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32815_Msal_Bot2012_COI	DRWB-036	1	BE32815_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	GYTAGTTCCRATGATGTTAGCTG	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32815_Mstr_Wil2021_COI	DRWB-036	1	BE32815_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	GTAGTGGTAACTACTCATGCTTTA	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32815_Pvir_Dia2013_COI	DRWB-036	1	BE32815_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	CTTAGTGGCATTAATTCGDAATCC	130.84724
BE32815	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52773	WGS84	BE32815	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32815_Rhar_Sim2018_COI	DRWB-036	1	BE32815_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32815	Sample	BE32815	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-036	COI	45	BE32815	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-036	CTGCTGCTATTGCTCATG	130.84724
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32817_Aamu_Bax2006_COI	DRWB-038	1	BE32817_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	GCACAACCGGGATCTTTACTTC	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32817_Pcan_Bot2011_IGS	DRWB-038	1	BE32817_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-038	IGS	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	CGTAATCCTCAGTACTGGCTA	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32817_Pper_Dia2013_COI	DRWB-038	1	BE32817_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	CTTAGTGGCATTAATTCGDAATCC	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32817_Sspa_Oph2007_COI	DRWB-038	1	BE32817_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	GAAGGCCTTCCGATGATC	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32817_Upin_Bot2011_COI	DRWB-038	1	BE32817_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	CTTTAATTACAGCGTTTTTATTGTTGT	130.84991
BE32817	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52792	WGS84	BE32817	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32817_Vgib_Wil2025_COI	DRWB-038	1	BE32817_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32817	Sample	BE32817	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-038	COI	45	BE32817	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-038	CCCCTCTTTACAAGTTCTTTAGGGT	130.84991
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32818_Asen_Wil2025_COI	DRWB-039	1	BE32818_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	GTTGTTGTTACAACACATGCTCTTTTG	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32818_Cjap_Sim2018_COI	DRWB-039	1	BE32818_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32818_Esin_And2018_Cyb	DRWB-039	1	BE32818_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-039	Cytb	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	ACCCCTCCTCATATCCAACCA	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32818_Mgig_Bot2012_COI	DRWB-039	1	BE32818_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	TCTTATTCGTTGGAGACTTTATAACCCT	130.85119
BE32818	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52793	WGS84	BE32818	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32818_Mjap_Gib2022_COI	DRWB-039	1	BE32818_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32818	Sample	BE32818	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-039	COI	45	BE32818	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-039	AATTTAATTGTGACTAGGCATGGTCTG	130.85119
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32819_Cmae_Bot2011_COI	DRWB-040	1	BE32819_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	ATGAACAGTCTATCCTCCTTTAG	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32819_Hsan_Wil2021_COI	DRWB-040	1	BE32819_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	CCTGGGCCGGTATAGTAGGT	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32819_Mros_Gib2022_COI	DRWB-040	1	BE32819_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32819_Msal_Bot2012_COI	DRWB-040	1	BE32819_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	GYTAGTTCCRATGATGTTAGCTG	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32819_Mstr_Wil2021_COI	DRWB-040	1	BE32819_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	GTAGTGGTAACTACTCATGCTTTA	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32819_Pvir_Dia2013_COI	DRWB-040	1	BE32819_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	CTTAGTGGCATTAATTCGDAATCC	130.85255
BE32819	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52808	WGS84	BE32819	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32819_Rhar_Sim2018_COI	DRWB-040	1	BE32819_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32819	Sample	BE32819	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-040	COI	45	BE32819	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-040	CTGCTGCTATTGCTCATG	130.85255
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32821_Asen_Wil2025_COI	DRWB-042	1	BE32821_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	GTTGTTGTTACAACACATGCTCTTTTG	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32821_Cjap_Sim2018_COI	DRWB-042	1	BE32821_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32821_Esin_And2018_Cyb	DRWB-042	1	BE32821_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-042	Cytb	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	ACCCCTCCTCATATCCAACCA	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32821_Mgig_Bot2012_COI	DRWB-042	1	BE32821_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	TCTTATTCGTTGGAGACTTTATAACCCT	130.84791
BE32821	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52663	WGS84	BE32821	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32821_Mjap_Gib2022_COI	DRWB-042	1	BE32821_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32821	Sample	BE32821	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-042	COI	45	BE32821	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-042	AATTTAATTGTGACTAGGCATGGTCTG	130.84791
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32822_Cmae_Bot2011_COI	DRWB-043	1	BE32822_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	ATGAACAGTCTATCCTCCTTTAG	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32822_Hsan_Wil2021_COI	DRWB-043	1	BE32822_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	CCTGGGCCGGTATAGTAGGT	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32822_Mros_Gib2022_COI	DRWB-043	1	BE32822_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32822_Msal_Bot2012_COI	DRWB-043	1	BE32822_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	GYTAGTTCCRATGATGTTAGCTG	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32822_Mstr_Wil2021_COI	DRWB-043	1	BE32822_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	GTAGTGGTAACTACTCATGCTTTA	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32822_Pvir_Dia2013_COI	DRWB-043	1	BE32822_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	CTTAGTGGCATTAATTCGDAATCC	130.84921
BE32822	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52668	WGS84	BE32822	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32822_Rhar_Sim2018_COI	DRWB-043	1	BE32822_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32822	Sample	BE32822	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-043	COI	45	BE32822	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-043	CTGCTGCTATTGCTCATG	130.84921
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32824_Aamu_Bax2006_COI	DRWB-045	1	BE32824_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	GCACAACCGGGATCTTTACTTC	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32824_Pcan_Bot2011_IGS	DRWB-045	1	BE32824_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-045	IGS	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	CGTAATCCTCAGTACTGGCTA	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32824_Pper_Dia2013_COI	DRWB-045	1	BE32824_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	CTTAGTGGCATTAATTCGDAATCC	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32824_Sspa_Oph2007_COI	DRWB-045	1	BE32824_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	GAAGGCCTTCCGATGATC	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32824_Upin_Bot2011_COI	DRWB-045	1	BE32824_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	CTTTAATTACAGCGTTTTTATTGTTGT	130.8519
BE32824	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52678	WGS84	BE32824	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32824_Vgib_Wil2025_COI	DRWB-045	1	BE32824_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32824	Sample	BE32824	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-045	COI	45	BE32824	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-045	CCCCTCTTTACAAGTTCTTTAGGGT	130.8519
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32825_Asen_Wil2025_COI	DRWB-046	1	BE32825_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	GTTGTTGTTACAACACATGCTCTTTTG	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32825_Cjap_Sim2018_COI	DRWB-046	1	BE32825_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32825_Esin_And2018_Cyb	DRWB-046	1	BE32825_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-046	Cytb	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	ACCCCTCCTCATATCCAACCA	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32825_Mgig_Bot2012_COI	DRWB-046	1	BE32825_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	TCTTATTCGTTGGAGACTTTATAACCCT	130.85325
BE32825	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52672	WGS84	BE32825	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32825_Mjap_Gib2022_COI	DRWB-046	1	BE32825_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32825	Sample	BE32825	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-046	COI	45	BE32825	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-046	AATTTAATTGTGACTAGGCATGGTCTG	130.85325
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32826_Cmae_Bot2011_COI	DRWB-047	1	BE32826_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	ATGAACAGTCTATCCTCCTTTAG	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32826_Hsan_Wil2021_COI	DRWB-047	1	BE32826_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	CCTGGGCCGGTATAGTAGGT	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32826_Mros_Gib2022_COI	DRWB-047	1	BE32826_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32826_Msal_Bot2012_COI	DRWB-047	1	BE32826_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	GYTAGTTCCRATGATGTTAGCTG	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32826_Mstr_Wil2021_COI	DRWB-047	1	BE32826_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	GTAGTGGTAACTACTCATGCTTTA	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32826_Pvir_Dia2013_COI	DRWB-047	1	BE32826_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	CTTAGTGGCATTAATTCGDAATCC	130.84593
BE32826	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52541	WGS84	BE32826	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32826_Rhar_Sim2018_COI	DRWB-047	1	BE32826_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32826	Sample	BE32826	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-047	COI	45	BE32826	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-047	CTGCTGCTATTGCTCATG	130.84593
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32828_Aamu_Bax2006_COI	DRWB-049	1	BE32828_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	GCACAACCGGGATCTTTACTTC	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32828_Pcan_Bot2011_IGS	DRWB-049	1	BE32828_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-049	IGS	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	CGTAATCCTCAGTACTGGCTA	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32828_Pper_Dia2013_COI	DRWB-049	1	BE32828_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	CTTAGTGGCATTAATTCGDAATCC	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32828_Sspa_Oph2007_COI	DRWB-049	1	BE32828_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	GAAGGCCTTCCGATGATC	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32828_Upin_Bot2011_COI	DRWB-049	1	BE32828_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	CTTTAATTACAGCGTTTTTATTGTTGT	130.84536
BE32828	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32828	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32828_Vgib_Wil2025_COI	DRWB-049	1	BE32828_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32828	Sample	BE32828	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-049	COI	45	BE32828	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-049	CCCCTCTTTACAAGTTCTTTAGGGT	130.84536
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32829_Asen_Wil2025_COI	DRWB-050	1	BE32829_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	GTTGTTGTTACAACACATGCTCTTTTG	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32829_Cjap_Sim2018_COI	DRWB-050	1	BE32829_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32829_Esin_And2018_Cyb	DRWB-050	1	BE32829_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-050	Cytb	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	ACCCCTCCTCATATCCAACCA	130.84662
BE32829	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52419	WGS84	BE32829	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32829_Mjap_Gib2022_COI	DRWB-050	1	BE32829_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32829	Sample	BE32829	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-050	COI	45	BE32829	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-050	AATTTAATTGTGACTAGGCATGGTCTG	130.84662
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32831_Aamu_Bax2006_COI	DRWB-052	1	BE32831_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	GCACAACCGGGATCTTTACTTC	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32831_Pcan_Bot2011_IGS	DRWB-052	1	BE32831_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-052	IGS	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	CGTAATCCTCAGTACTGGCTA	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32831_Pper_Dia2013_COI	DRWB-052	1	BE32831_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	CTTAGTGGCATTAATTCGDAATCC	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32831_Sspa_Oph2007_COI	DRWB-052	1	BE32831_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	GAAGGCCTTCCGATGATC	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32831_Upin_Bot2011_COI	DRWB-052	1	BE32831_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	CTTTAATTACAGCGTTTTTATTGTTGT	130.85133
BE32831	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52487	WGS84	BE32831	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32831_Vgib_Wil2025_COI	DRWB-052	1	BE32831_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32831	Sample	BE32831	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-052	COI	45	BE32831	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-052	CCCCTCTTTACAAGTTCTTTAGGGT	130.85133
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32832_Asen_Wil2025_COI	DRWB-053	1	BE32832_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	GTTGTTGTTACAACACATGCTCTTTTG	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32832_Cjap_Sim2018_COI	DRWB-053	1	BE32832_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32832_Esin_And2018_Cyb	DRWB-053	1	BE32832_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-053	Cytb	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	ACCCCTCCTCATATCCAACCA	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32832_Mgig_Bot2012_COI	DRWB-053	1	BE32832_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	TCTTATTCGTTGGAGACTTTATAACCCT	130.84465
BE32832	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52322	WGS84	BE32832	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32832_Mjap_Gib2022_COI	DRWB-053	1	BE32832_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32832	Sample	BE32832	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-053	COI	45	BE32832	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-053	AATTTAATTGTGACTAGGCATGGTCTG	130.84465
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32833_Cmae_Bot2011_COI	DRWB-054	1	BE32833_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	ATGAACAGTCTATCCTCCTTTAG	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32833_Hsan_Wil2021_COI	DRWB-054	1	BE32833_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	CCTGGGCCGGTATAGTAGGT	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32833_Mros_Gib2022_COI	DRWB-054	1	BE32833_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32833_Msal_Bot2012_COI	DRWB-054	1	BE32833_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	GYTAGTTCCRATGATGTTAGCTG	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32833_Mstr_Wil2021_COI	DRWB-054	1	BE32833_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	GTAGTGGTAACTACTCATGCTTTA	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32833_Pvir_Dia2013_COI	DRWB-054	1	BE32833_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	CTTAGTGGCATTAATTCGDAATCC	130.84601
BE32833	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52324	WGS84	BE32833	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32833_Rhar_Sim2018_COI	DRWB-054	1	BE32833_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32833	Sample	BE32833	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-054	COI	45	BE32833	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-054	CTGCTGCTATTGCTCATG	130.84601
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32835_Aamu_Bax2006_COI	DRWB-056	1	BE32835_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	GCACAACCGGGATCTTTACTTC	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32835_Pcan_Bot2011_IGS	DRWB-056	1	BE32835_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-056	IGS	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	CGTAATCCTCAGTACTGGCTA	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32835_Pper_Dia2013_COI	DRWB-056	1	BE32835_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	CTTAGTGGCATTAATTCGDAATCC	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32835_Sspa_Oph2007_COI	DRWB-056	1	BE32835_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	GAAGGCCTTCCGATGATC	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32835_Upin_Bot2011_COI	DRWB-056	1	BE32835_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	CTTTAATTACAGCGTTTTTATTGTTGT	130.84535
BE32835	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.5221	WGS84	BE32835	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32835_Vgib_Wil2025_COI	DRWB-056	1	BE32835_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32835	Sample	BE32835	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-056	COI	45	BE32835	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-056	CCCCTCTTTACAAGTTCTTTAGGGT	130.84535
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32836_Asen_Wil2025_COI	DRWB-057	1	BE32836_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	GTTGTTGTTACAACACATGCTCTTTTG	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32836_Cjap_Sim2018_COI	DRWB-057	1	BE32836_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32836_Esin_And2018_Cyb	DRWB-057	1	BE32836_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-057	Cytb	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	ACCCCTCCTCATATCCAACCA	130.84667
BE32836	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52208	WGS84	BE32836	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32836_Mjap_Gib2022_COI	DRWB-057	1	BE32836_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32836	Sample	BE32836	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-057	COI	45	BE32836	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-057	AATTTAATTGTGACTAGGCATGGTCTG	130.84667
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32837_Cmae_Bot2011_COI	DRWB-058	1	BE32837_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	ATGAACAGTCTATCCTCCTTTAG	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32837_Hsan_Wil2021_COI	DRWB-058	1	BE32837_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	CCTGGGCCGGTATAGTAGGT	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32837_Mros_Gib2022_COI	DRWB-058	1	BE32837_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32837_Msal_Bot2012_COI	DRWB-058	1	BE32837_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	GYTAGTTCCRATGATGTTAGCTG	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32837_Mstr_Wil2021_COI	DRWB-058	1	BE32837_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	GTAGTGGTAACTACTCATGCTTTA	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32837_Pvir_Dia2013_COI	DRWB-058	1	BE32837_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	CTTAGTGGCATTAATTCGDAATCC	130.85111
BE32837	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52176	WGS84	BE32837	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32837_Rhar_Sim2018_COI	DRWB-058	1	BE32837_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32837	Sample	BE32837	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-058	COI	45	BE32837	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-058	CTGCTGCTATTGCTCATG	130.85111
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32839_Aamu_Bax2006_COI	DRWB-060	1	BE32839_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	GCACAACCGGGATCTTTACTTC	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32839_Pcan_Bot2011_IGS	DRWB-060	1	BE32839_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-060	IGS	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	CGTAATCCTCAGTACTGGCTA	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32839_Pper_Dia2013_COI	DRWB-060	1	BE32839_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	CTTAGTGGCATTAATTCGDAATCC	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32839_Sspa_Oph2007_COI	DRWB-060	1	BE32839_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	GAAGGCCTTCCGATGATC	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32839_Upin_Bot2011_COI	DRWB-060	1	BE32839_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	CTTTAATTACAGCGTTTTTATTGTTGT	130.84601
BE32839	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52097	WGS84	BE32839	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.3)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32839_Vgib_Wil2025_COI	DRWB-060	1	BE32839_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32839	Sample	BE32839	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-060	COI	45	BE32839	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-060	CCCCTCTTTACAAGTTCTTTAGGGT	130.84601
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32840_Cmae_Bot2011_COI	DRWB-061	1	BE32840_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	ATGAACAGTCTATCCTCCTTTAG	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32840_Hsan_Wil2021_COI	DRWB-061	1	BE32840_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	CCTGGGCCGGTATAGTAGGT	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32840_Mros_Gib2022_COI	DRWB-061	1	BE32840_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32840_Msal_Bot2012_COI	DRWB-061	1	BE32840_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	GYTAGTTCCRATGATGTTAGCTG	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32840_Mstr_Wil2021_COI	DRWB-061	1	BE32840_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	GTAGTGGTAACTACTCATGCTTTA	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32840_Pvir_Dia2013_COI	DRWB-061	1	BE32840_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	CTTAGTGGCATTAATTCGDAATCC	130.84739
BE32840	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52098	WGS84	BE32840	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32840_Rhar_Sim2018_COI	DRWB-061	1	BE32840_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32840	Sample	BE32840	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-061	COI	45	BE32840	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-061	CTGCTGCTATTGCTCATG	130.84739
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32842_Aamu_Bax2006_COI	DRWB-063	1	BE32842_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	GCACAACCGGGATCTTTACTTC	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32842_Pcan_Bot2011_IGS	DRWB-063	1	BE32842_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-063	IGS	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	CGTAATCCTCAGTACTGGCTA	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32842_Pper_Dia2013_COI	DRWB-063	1	BE32842_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	CTTAGTGGCATTAATTCGDAATCC	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32842_Sspa_Oph2007_COI	DRWB-063	1	BE32842_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	GAAGGCCTTCCGATGATC	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32842_Upin_Bot2011_COI	DRWB-063	1	BE32842_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	CTTTAATTACAGCGTTTTTATTGTTGT	130.85008
BE32842	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.52101	WGS84	BE32842	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Nil (Scale factor = 1)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32842_Vgib_Wil2025_COI	DRWB-063	1	BE32842_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32842	Sample	BE32842	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-063	COI	45	BE32842	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-063	CCCCTCTTTACAAGTTCTTTAGGGT	130.85008
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32843_Asen_Wil2025_COI	DRWB-064	1	BE32843_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	GTTGTTGTTACAACACATGCTCTTTTG	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32843_Cjap_Sim2018_COI	DRWB-064	1	BE32843_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32843_Esin_And2018_Cyb	DRWB-064	1	BE32843_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-064	Cytb	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	ACCCCTCCTCATATCCAACCA	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32843_Mgig_Bot2012_COI	DRWB-064	1	BE32843_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	TCTTATTCGTTGGAGACTTTATAACCCT	130.84544
BE32843	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51983	WGS84	BE32843	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32843_Mjap_Gib2022_COI	DRWB-064	1	BE32843_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32843	Sample	BE32843	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-064	COI	45	BE32843	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-064	AATTTAATTGTGACTAGGCATGGTCTG	130.84544
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32844_Cmae_Bot2011_COI	DRWB-065	1	BE32844_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	ATGAACAGTCTATCCTCCTTTAG	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32844_Hsan_Wil2021_COI	DRWB-065	1	BE32844_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	CCTGGGCCGGTATAGTAGGT	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32844_Mros_Gib2022_COI	DRWB-065	1	BE32844_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32844_Msal_Bot2012_COI	DRWB-065	1	BE32844_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	GYTAGTTCCRATGATGTTAGCTG	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32844_Mstr_Wil2021_COI	DRWB-065	1	BE32844_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	GTAGTGGTAACTACTCATGCTTTA	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32844_Pvir_Dia2013_COI	DRWB-065	1	BE32844_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	CTTAGTGGCATTAATTCGDAATCC	130.84676
BE32844	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51986	WGS84	BE32844	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.5)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32844_Rhar_Sim2018_COI	DRWB-065	1	BE32844_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32844	Sample	BE32844	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-065	COI	45	BE32844	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-065	CTGCTGCTATTGCTCATG	130.84676
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	TCATGCTCTTGTAATGATAT	FAM	qPCR	Material Sample	""	50	Asterias amurensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTCATTTACCAAATCCTCCTATCATAATA	marine	Asterias amurensis	92.3	122	BE32846_Aamu_Bax2006_COI	DRWB-067	1	BE32846_Aamu_Bax2006_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Aamu_Bax2006_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	GCACAACCGGGATCTTTACTTC	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	ATAGAGTAGAGCTATTTAGGG	FAM	qPCR	Material Sample	""	50	Perna canaliculus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CTCTAACATAAGGGCTCTAAC	marine	Perna canaliculus	95.2	162	BE32846_Pcan_Bot2011_IGS	DRWB-067	1	BE32846_Pcan_Bot2011_IGS	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Pcan_Bot2011_IGS	DRWB-067	IGS	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	CGTAATCCTCAGTACTGGCTA	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	AACCATCGACTCAATTAA	FAM	qPCR	Material Sample	""	50	Perna perna	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna perna	93.5	281	BE32846_Pper_Dia2013_COI	DRWB-067	1	BE32846_Pper_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Pper_Dia2013_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	CTTAGTGGCATTAATTCGDAATCC	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	CTCGCTCCGCCTCG	FAM	qPCR	Material Sample	""	50	Sabella spallanzanii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAGGCATAGTTCACCATCTTTCG	marine	Sabella spallanzanii	95.7	139	BE32846_Sspa_Oph2007_COI	DRWB-067	1	BE32846_Sspa_Oph2007_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Sspa_Oph2007_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	GAAGGCCTTCCGATGATC	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	CGGTTTTAGCAGGTGCT	FAM	qPCR	Material Sample	""	50	Undaria pinnatifida	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AGTAGTATTAAAATTACGATCTGTTAGT	marine	Undaria pinnatifida	95.3	84	BE32846_Upin_Bot2011_COI	DRWB-067	1	BE32846_Upin_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Upin_Bot2011_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	CTTTAATTACAGCGTTTTTATTGTTGT	130.84942
BE32846	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51991	WGS84	BE32846	TACTAGGGCTTTACTGATTG	FAM	qPCR	Material Sample	""	50	Varicorbula gibba	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.4)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TCCTGCTAGAACTGGCATTGC	marine	Varicorbula gibba	97.8	69	BE32846_Vgib_Wil2025_COI	DRWB-067	1	BE32846_Vgib_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32846	Sample	BE32846	targeted	Absent	Sample	Vgib_Wil2025_COI	DRWB-067	COI	45	BE32846	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-067	CCCCTCTTTACAAGTTCTTTAGGGT	130.84942
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	TTTGGTAATTGGCTGATTC	FAM	qPCR	Material Sample	""	50	Arcuatula senhousia	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	TTTAGACGAGGGAACACTAAGTCACC	marine	Arcuatula senhousia	91.8	126	BE32847_Asen_Wil2025_COI	DRWB-068	1	BE32847_Asen_Wil2025_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Asen_Wil2025_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	GTTGTTGTTACAACACATGCTCTTTTG	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	TATTACTGCCATTCTTCTAC	FAM	qPCR	Material Sample	""	50	Charybdis japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGTTTCGGTCTGTTAATAATATAGTAATAGCT	marine	Charybdis japonica	97.7	139	BE32847_Cjap_Sim2018_COI	DRWB-068	1	BE32847_Cjap_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Cjap_Sim2018_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	TTAATATACGGTCATTTGGTATGAGTATAGATC	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	TTTGCTTACGCTATTTTACGATCAATTCCT	FAM	qPCR	Material Sample	""	50	Eriocheir sinensis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	BHQ	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AAGAATGGCCACTGAAGCGG	marine	Eriocheir sinensis	98.8	114	BE32847_Esin_And2018_Cyb	DRWB-068	1	BE32847_Esin_And2018_Cyb	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Esin_And2018_Cyb	DRWB-068	Cytb	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	ACCCCTCCTCATATCCAACCA	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	CCCCGTGACTTATAATG	FAM	qPCR	Material Sample	""	50	Magallana gigas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ATAACCAACGCATGCCTAGTTAC	marine	Magallana gigas	94.8	90	BE32847_Mgig_Bot2012_COI	DRWB-068	1	BE32847_Mgig_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Mgig_Bot2012_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	TCTTATTCGTTGGAGACTTTATAACCCT	130.84744
BE32847	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51879	WGS84	BE32847	CTATAATAATAGGTGGATTTGGG	FAM	qPCR	Material Sample	""	50	Mya japonica	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.9)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CATTCGGGGAAAAGCTATATCG	marine	Mya japonica	97.4	129	BE32847_Mjap_Gib2022_COI	DRWB-068	1	BE32847_Mjap_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32847	Sample	BE32847	targeted	Absent	Sample	Mjap_Gib2022_COI	DRWB-068	COI	45	BE32847	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-068	AATTTAATTGTGACTAGGCATGGTCTG	130.84744
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	AGTTGATTTAGGGATTTTC	FAM	qPCR	Material Sample	""	50	Carcinus maenas	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	GAAAGAACGCATATTGATAATAGTTG	marine	Carcinus maenas	95.1	147	BE32848_Cmae_Bot2011_COI	DRWB-069	1	BE32848_Cmae_Bot2011_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Cmae_Bot2011_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	ATGAACAGTCTATCCTCCTTTAG	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	ATCATTACCAATTAAGCTTC	FAM	qPCR	Material Sample	""	50	Hemigrapsus sanguineus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGAAAGCATGAGCTGTAACAA	marine	Hemigrapsus sanguineus	95.2	120	BE32848_Hsan_Wil2021_COI	DRWB-069	1	BE32848_Hsan_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Hsan_Wil2021_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	CCTGGGCCGGTATAGTAGGT	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	TGGGTGGACCGTGTAT	FAM	qPCR	Material Sample	""	50	Maoricolpus roseus	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CCCCGCATGCGCAAT	marine	Maoricolpus roseus	98.6	112	BE32848_Mros_Gib2022_COI	DRWB-069	1	BE32848_Mros_Gib2022_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Mros_Gib2022_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	ACTTCTACCTCCTGCTCTTCTACTTCTC	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	CCTCGGCTTAATAATGTTAGT	FAM	qPCR	Material Sample	""	50	Mytilopsis sallei	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	ACCTATTGAAACAGGCAACAGGCAACACTC	marine	Mytilopsis sallei	97	88	BE32848_Msal_Bot2012_COI	DRWB-069	1	BE32848_Msal_Bot2012_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Msal_Bot2012_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	GYTAGTTCCRATGATGTTAGCTG	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	CGGTGCATTTGGGAATTGACTTCTTCC	FAM	qPCR	Material Sample	""	50	Mytella strigata	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	AATCACAACCACCGATACATAA	marine	Mytella strigata	93	109	BE32848_Mstr_Wil2021_COI	DRWB-069	1	BE32848_Mstr_Wil2021_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Mstr_Wil2021_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	GTAGTGGTAACTACTCATGCTTTA	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	ACTCAAACAACAAAGTAAAC	FAM	qPCR	Material Sample	""	50	Perna viridis	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CAAAGTACCAATATCTTTATGATTRGTWGA	marine	Perna viridis	95.6	200	BE32848_Pvir_Dia2013_COI	DRWB-069	1	BE32848_Pvir_Dia2013_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Pvir_Dia2013_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	CTTAGTGGCATTAATTCGDAATCC	130.84876
BE32848	single technical replicate positive with normal amplification within 45 cycles	SARDI_MDL_16427	-12.51872	WGS84	BE32848	AGCCTCCGTTGATA	FAM	qPCR	Material Sample	""	50	Rhithropanopeus harrisii	Plankton filtered onto 0.45 micron filter and freeze-dried	Negligible (Scale factor = 1.8)	MGB	Wiltshire KH, Giblot-Ducray D, Hill K, Deveney MR (in prep.) Molecular surveys for priority marine pests in 2023-24 for Gladstone, Townsville and Cairns, Queensland, and Darwin, Northern Territory.  Report to the Department of Agriculture, Fisheries and Forestry South Australian Research and Development Institute (Aquatic Sciences), Adelaide.	2023-24_Molecular_surveys-DRW01_23-24_B	3/05/2024	CGGCACCTAAAATAGAAGAAA	marine	Rhithropanopeus harrisii	99.8	87	BE32848_Rhar_Sim2018_COI	DRWB-069	1	BE32848_Rhar_Sim2018_COI	Plankton tow	100 m surface tow with 150 micron Plankton net	BE32848	Sample	BE32848	targeted	Absent	Sample	Rhar_Sim2018_COI	DRWB-069	COI	45	BE32848	1.00E-05	DNA yield of an internal control was compared between the sample and reference samples. A scale factor calculated as the ratio of internal control DNA in the sample to the reference	Australia	DRWB-069	CTGCTGCTATTGCTCATG	130.84876
